LOCUS       DAXWOO010000001        55987 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.001, whole genome shotgun sequence.
ACCESSION   DAXWOO010000001 DAXWOO010000000
VERSION     DAXWOO010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.001"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            29..1660
                     /locus_tag="ACGE70_000001"
     CDS             29..1660
                     /locus_tag="ACGE70_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFF8691676.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(1722..2363)
                     /locus_tag="ACGE70_000002"
     CDS             complement(1722..2363)
                     /locus_tag="ACGE70_000002"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFF8691677.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVPPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    2549..2625
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            2645..2953
                     /gene="rplU"
                     /locus_tag="ACGE70_000003"
     CDS             2645..2953
                     /gene="rplU"
                     /locus_tag="ACGE70_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFF8691678.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            2980..3324
                     /locus_tag="ACGE70_000004"
     CDS             2980..3324
                     /locus_tag="ACGE70_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFF8691679.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            3337..3630
                     /gene="rpmA"
                     /locus_tag="ACGE70_000005"
     CDS             3337..3630
                     /gene="rpmA"
                     /locus_tag="ACGE70_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFF8691680.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            4064..6871
                     /locus_tag="ACGE70_000006"
     CDS             4064..6871
                     /locus_tag="ACGE70_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFF8691681.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            7062..7553
                     /locus_tag="ACGE70_000007"
     CDS             7062..7553
                     /locus_tag="ACGE70_000007"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFF8691682.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            7656..8645
                     /locus_tag="ACGE70_000008"
     CDS             7656..8645
                     /locus_tag="ACGE70_000008"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFF8691683.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            8678..9481
                     /locus_tag="ACGE70_000009"
     CDS             8678..9481
                     /locus_tag="ACGE70_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFF8691684.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            9502..10416
                     /locus_tag="ACGE70_000010"
     CDS             9502..10416
                     /locus_tag="ACGE70_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFF8691685.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            10499..10873
                     /locus_tag="ACGE70_000011"
     CDS             10499..10873
                     /locus_tag="ACGE70_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFF8691686.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            11012..11257
                     /locus_tag="ACGE70_000012"
     CDS             11012..11257
                     /locus_tag="ACGE70_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFF8691687.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            11429..12493
                     /locus_tag="ACGE70_000013"
     CDS             11429..12493
                     /locus_tag="ACGE70_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFF8691688.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            12739..13164
                     /locus_tag="ACGE70_000014"
     CDS             12739..13164
                     /locus_tag="ACGE70_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFF8691689.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            13157..13606
                     /gene="nusB"
                     /locus_tag="ACGE70_000015"
     CDS             13157..13606
                     /gene="nusB"
                     /locus_tag="ACGE70_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFF8691690.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            13703..14557
                     /locus_tag="ACGE70_000016"
     CDS             13703..14557
                     /locus_tag="ACGE70_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFF8691691.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            14558..15895
                     /gene="xseA"
                     /locus_tag="ACGE70_000017"
     CDS             14558..15895
                     /gene="xseA"
                     /locus_tag="ACGE70_000017"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFF8691692.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            15900..16124
                     /locus_tag="ACGE70_000018"
     CDS             15900..16124
                     /locus_tag="ACGE70_000018"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFF8691693.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            16126..17016
                     /locus_tag="ACGE70_000019"
     CDS             16126..17016
                     /locus_tag="ACGE70_000019"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFF8691694.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            17025..17843
                     /locus_tag="ACGE70_000020"
     CDS             17025..17843
                     /locus_tag="ACGE70_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFF8691695.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVASNAHEQFDHK"
     gene            17950..18399
                     /locus_tag="ACGE70_000021"
     CDS             17950..18399
                     /locus_tag="ACGE70_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFF8691696.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            18417..20099
                     /gene="recN"
                     /locus_tag="ACGE70_000022"
     CDS             18417..20099
                     /gene="recN"
                     /locus_tag="ACGE70_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFF8691697.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(20154..20270)
                     /locus_tag="ACGE70_000023"
     CDS             complement(20154..20270)
                     /locus_tag="ACGE70_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFF8691698.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(20343..21299)
                     /locus_tag="ACGE70_000024"
     CDS             complement(20343..21299)
                     /locus_tag="ACGE70_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFF8691699.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            21470..21838
                     /locus_tag="ACGE70_000025"
     CDS             21470..21838
                     /locus_tag="ACGE70_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFF8691700.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            complement(22041..23354)
                     /locus_tag="ACGE70_000026"
     CDS             complement(22041..23354)
                     /locus_tag="ACGE70_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004271296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HFF8691701.1"
                     /translation="MATLHEKKVQFNSKLTISNTGGNLSTDSGLVLVKEFMESLNFSD
                     LSKQYLGIEDKRLYHIHDNFSLMEQLIYQNIAGYSTDSSANILKQDPIFKVILDKSRL
                     ASQSSISRFWDRISEENISQLQDLNQAMIDKVRSARNTTEMIFDLDSTHSDTYGDQEN
                     TDYNAHYRTNGYHPLVAFDGLTGDFLKAELRSGNVYTSNGVGTFMEPLFEHYNQVVPV
                     SNILVRGDSGFATPELYDLCEAYDSFFVIRLKANRNLSKIAESFIQIDDNHPWDKKEV
                     VYSSTSYQAKSWSKERRVCIKSTREADELLFRHEYIVTNYSNNVSAETVFRTYCKRGT
                     MENFIKEAKNGFYFDKTDSPSFLENHARMMVSLLAYNIVNFMRTLCFTQGSASMQVDT
                     IRLKLFKVAGKLVRTGRRLLLKLSSYHLHQGLFYQVLGNIQQLCW"
     gene            23672..24103
                     /gene="mraZ"
                     /locus_tag="ACGE70_000027"
     CDS             23672..24103
                     /gene="mraZ"
                     /locus_tag="ACGE70_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFF8691702.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            24124..25083
                     /gene="rsmH"
                     /locus_tag="ACGE70_000028"
     CDS             24124..25083
                     /gene="rsmH"
                     /locus_tag="ACGE70_000028"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFF8691703.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            25088..25489
                     /gene="ftsL"
                     /locus_tag="ACGE70_000029"
     CDS             25088..25489
                     /gene="ftsL"
                     /locus_tag="ACGE70_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFF8691704.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            25490..27682
                     /locus_tag="ACGE70_000030"
     CDS             25490..27682
                     /locus_tag="ACGE70_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFF8691705.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGIAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            27708..28670
                     /gene="mraY"
                     /locus_tag="ACGE70_000031"
     CDS             27708..28670
                     /gene="mraY"
                     /locus_tag="ACGE70_000031"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFF8691706.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            28684..30045
                     /gene="murD"
                     /locus_tag="ACGE70_000032"
     CDS             28684..30045
                     /gene="murD"
                     /locus_tag="ACGE70_000032"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFF8691707.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            30071..31159
                     /gene="murG"
                     /locus_tag="ACGE70_000033"
     CDS             30071..31159
                     /gene="murG"
                     /locus_tag="ACGE70_000033"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFF8691708.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            31174..32394
                     /locus_tag="ACGE70_000034"
     CDS             31174..32394
                     /locus_tag="ACGE70_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFF8691709.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            32550..33875
                     /gene="ftsA"
                     /locus_tag="ACGE70_000035"
     CDS             32550..33875
                     /gene="ftsA"
                     /locus_tag="ACGE70_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFF8691710.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            33898..35139
                     /gene="ftsZ"
                     /locus_tag="ACGE70_000036"
     CDS             33898..35139
                     /gene="ftsZ"
                     /locus_tag="ACGE70_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFF8691711.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            35156..35833
                     /locus_tag="ACGE70_000037"
     CDS             35156..35833
                     /locus_tag="ACGE70_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFF8691712.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            35847..36446
                     /locus_tag="ACGE70_000038"
     CDS             35847..36446
                     /locus_tag="ACGE70_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFF8691713.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            36527..36748
                     /locus_tag="ACGE70_000039"
     CDS             36527..36748
                     /locus_tag="ACGE70_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFF8691714.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            36797..37579
                     /locus_tag="ACGE70_000040"
     CDS             36797..37579
                     /locus_tag="ACGE70_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFF8691715.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            37600..38307
                     /locus_tag="ACGE70_000041"
     CDS             37600..38307
                     /locus_tag="ACGE70_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFF8691716.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            38569..41367
                     /gene="ileS"
                     /locus_tag="ACGE70_000042"
     CDS             38569..41367
                     /gene="ileS"
                     /locus_tag="ACGE70_000042"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFF8691717.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKSYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(41416..42942)
                     /gene="zwf"
                     /locus_tag="ACGE70_000043"
     CDS             complement(41416..42942)
                     /gene="zwf"
                     /locus_tag="ACGE70_000043"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFF8691718.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(42957..43604)
                     /locus_tag="ACGE70_000044"
     CDS             complement(42957..43604)
                     /locus_tag="ACGE70_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFF8691719.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(43788..44117)
                     /locus_tag="ACGE70_000045"
     CDS             complement(43788..44117)
                     /locus_tag="ACGE70_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFF8691720.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            44294..45022
                     /gene="sfsA"
                     /locus_tag="ACGE70_000046"
     CDS             44294..45022
                     /gene="sfsA"
                     /locus_tag="ACGE70_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFF8691721.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            45038..46051
                     /locus_tag="ACGE70_000047"
     CDS             45038..46051
                     /locus_tag="ACGE70_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFF8691722.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            46051..47328
                     /locus_tag="ACGE70_000048"
     CDS             46051..47328
                     /locus_tag="ACGE70_000048"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFF8691723.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            47391..50093
                     /locus_tag="ACGE70_000049"
     CDS             47391..50093
                     /locus_tag="ACGE70_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFF8691724.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            50245..50562
                     /locus_tag="ACGE70_000050"
     CDS             50245..50562
                     /locus_tag="ACGE70_000050"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFF8691725.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            50592..50912
                     /locus_tag="ACGE70_000051"
     CDS             50592..50912
                     /locus_tag="ACGE70_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFF8691726.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            51020..52480
                     /locus_tag="ACGE70_000052"
     CDS             51020..52480
                     /locus_tag="ACGE70_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFF8691727.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            52548..52769
                     /locus_tag="ACGE70_000053"
     CDS             52548..52769
                     /locus_tag="ACGE70_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8691728.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            52800..52982
                     /locus_tag="ACGE70_000054"
     CDS             52800..52982
                     /locus_tag="ACGE70_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFF8691729.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            52982..53395
                     /locus_tag="ACGE70_000055"
     CDS             52982..53395
                     /locus_tag="ACGE70_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFF8691730.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            53518..54699
                     /locus_tag="ACGE70_000056"
     CDS             53518..54699
                     /locus_tag="ACGE70_000056"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8691731.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(54770..54943)
                     /locus_tag="ACGE70_000057"
     CDS             complement(54770..54943)
                     /locus_tag="ACGE70_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8691732.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTPKTKE
                     NKKFKFTKKEDLL"
     gene            55097..55170
                     /locus_tag="ACGE70_000058"
     tRNA            55097..55170
                     /locus_tag="ACGE70_000058"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:55131..55133,aa:Arg,seq:cct)
     gene            complement(55239..>55987)
                     /locus_tag="ACGE70_000059"
     CDS             complement(55239..>55987)
                     /locus_tag="ACGE70_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFF8691733.1"
                     /translation="HIRASLKDAVEEGLIPFDPTRKAVIKGKDSLKPKEDKYLDYDQF
                     KSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDIDFEDQIITINKTWNYK
                     LNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIKNPHNFVFFNLKNGLV
                     SSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNILSVSKRLGHSSLETTM
                     STYLHIVRELEDQDKEKINTVFDSLYKNDN"
BASE COUNT        18396 a         9537 c        12051 g        16003 t
ORIGIN      
        1 aaaatttcat gaaaaaggag ttagataggt gttcaaaaat tatgtttcct cactaaaaaa
       61 agaattttca ggttatgatc tttcaagatt tcagaaagat cttttggctg ggatcacagt
      121 agctgctgta gcattgcctt tagccttggc ttttggggta tcaagcggag ctgatgcggc
      181 cgcaggtttg attacggcta tcattgccgg tatcgttatt ggcggtttat caggcggttt
      241 ttatcaaata tcaggtccga caggtgcgat ggcagctatc ttgatgtcta ttgcggcaac
      301 aaagggaatg caaggtgtat tgcttgctac ttttttagcg ggtctatttt tacttgcagc
      361 aggacttttc cgattaggaa cactgacttc ttttatccca gcacccgtaa ttacggggtt
      421 tacttcaggt attgctatca ttattgcatt aggtcagatc gataatttat ttggtgttca
      481 ctcggaaggt acaaacgtta ttgaaaaact aagtagttat caaacgatag gatttccgat
      541 aaacacagcc tctttgatca tgggagggct agtcatttta gggatgttta tctatccaaa
      601 aaaatgggcg aaacgaatgc ctagttcgct gctggctatc attttggcaa cagcagttat
      661 gctattgttc catttaccag tagcaactgt gggtaagatt ccacaaacat tgatcagtag
      721 taatcgtttg aatatgggag attttagctt ttctgccttg caacaagtgg cagttccagc
      781 tattagcatt gctttattag gaatgattga aagcttgcta tgtggtgctt cggctggccg
      841 aatggcaaat aagcccttag atagcaatca agaattaatg gcgcaaggaa ttggaaatct
      901 gttgcttcct ttctttggcg ggattcctgc tactgcggcc atcgcccgta caagcgtggc
      961 catcaaatct ggtgcacaaa ctagattagc tggtatgata catgcagttg tcttactgct
     1021 ctccatgctt gtttttgcgc cagtgatgtc acagattcca atgccagcac ttgcaggtgt
     1081 tttgattgtg acggcttggc gtatgaatga atgggaaacg atcaaggagc tatttactaa
     1141 taaatattgg tcagcagttc tattgtttat ccttacgatg atttgtacag ttatctttga
     1201 tttaagtatt gctatcgtga tcggggtgat cggcggctgt gtattttttg ttgtcaaaag
     1261 cgctgctatc acgatttcat tagaaacgat cgattggcaa agaatggggc tgccagaaac
     1321 ggaaaagctg aataactgga cagtagtgta tattagtggt ccactgtttt ttatgtcttc
     1381 agaaaaactc aaaaataaat tggaagaact aaatcatcac gatggtgtca ttttctccat
     1441 gcgaggcgta ccaagtattg atttgacggc acgtgcagtt tttgatgaat ttcaagaaaa
     1501 agcagagcaa agaggacaaa cgattatcta tacttccttg cagccagaag tagaaaaaca
     1561 actttctcac ttgtgggaac atacattcac gcatcaacat caaacagttg ctcatgcgtt
     1621 gacagcttta catgcgaaat atactgcaat tagccattaa aacaatgaaa aagaacagac
     1681 aagactgtaa actgaagatt ttatggtctt gtctgttctt tttaatattg ctgtaaaagt
     1741 gaagctagta cttgccagtc ttgatggata acaggtacaa gggagcgatt gtagaaaata
     1801 gctgctggat gaaaaagtgg aagaattcga tagttttttt ctgaccattc gtaggcatcc
     1861 ttttgttctg ttaattgtaa gacaggccct gtatagagtt gaccatgatt tttggtaacc
     1921 gtatattctt tgccaagtaa acgttgaaga ccaatgtttc caagtgtcgc aattaatttt
     1981 ggcgggacct tcatcagttc gtaatccaat attggtgcat gcgcaaatac ttcggaacga
     2041 gtaggtgtcc gatttggata aacagtttct gactgttgtg tccgtttatt gatccgatga
     2101 gtcacgcgat acgggcggct ccttacagca ctggtgatat acacctcttc tcgtgttaag
     2161 ccagttgaag aaagtgcctg catcaattct tttccagctt gaccgctaaa aggaacatgc
     2221 gtatcaatct ctgttttccc tggggcttct cccacgagca tcagtttagg atgaagaggt
     2281 ccttgcccag cgataaatcc ttctacagga cggccgttca ttcttaaaga agcttcttgt
     2341 atcagttctt ttgggtattc catttatgct cctccttttt cttttattat aagaataaaa
     2401 gaaacacaat gcgactattt tatttggtag aactgtgagg atagaaaagc agaagtattg
     2461 cttgaatttt tgttttactt tttgatttta aatttagttg actaacggtt gctgttctgg
     2521 taagctagta ctgttgtaat tgtggactcc acagctacaa ccgctcagaa caagtaataa
     2581 gcacagcata tgctgtcact tcggatggcg agtctaagta tatcagaagg aggtgcttta
     2641 tagcatgtac gcaattatta aaactggtgg taaacaagta aaagttgaag tgggacaagc
     2701 aatctatgtt gaaaaacttg acgttgaagc aggagaaaaa gttgtttttg acgaagttat
     2761 cttagtaggc ggagaatcta caaaagtagg cgctccaact gttgcaggtg caactgttga
     2821 aggtactgtt gaaaaacacg gcaaacaaaa gaaagtcgtt actttcaaat acaaacctaa
     2881 aaaacattca caccgtaaac aaggtcatcg tcaaccatat acaaaagtgg ttatcgacgc
     2941 gatcaacgct taatagcgtt tgagaaagaa ggcctatata tgatcaaagg aacatttaag
     3001 cgaaatgacg caggtcagat cgtttcattc actttgaccg ggcatgctga tgctggtcct
     3061 tatggaagtg atatcgtttg tgcgggtgtt tctgcgttag cgatcagcac tgtcaacggg
     3121 attgcctcat tagccggttt tgagccgatt gttgaaatga atgaagaaga aggcggttat
     3181 ctttataccg aagtcacttc aggaatgaca caagaacaaa acaatattgc tgagattcta
     3241 ttagaaaatc ttttattagg attacaatcg atagaagctg aaaactcaga atatatccaa
     3301 attaaaacaa ttaatgataa ataggaggtg cagaccatgt tgttatctat gaatctacaa
     3361 ttatttgccc acaaaaaagg tggcggttct acatctaacg gccgtgactc agaatcaaaa
     3421 cgtctaggtg ctaaacgtgc tgatggacaa acagttacag gtggatcaat tctttaccgt
     3481 caacgcggta caaaaatcta tccaggtgta aacgtgggta tcggtggaga cgacacttta
     3541 tttgcaaaag ttgatggcgt agtacgtttc gaacgtaaag gccgtaacaa aaaacaagtg
     3601 tctgtttatc cagtagctca agaagcttaa ttgaatctta tgattaaaga ggttgtgaca
     3661 agacttttgt cacagccttt tttgtttata aaaataaaag ttgtagtgga ggctacgaag
     3721 tttactttgt ctttttacac ttaatatttt tttgccaatt tggaatcagg atgttttgat
     3781 aactatcgtt ttcaattgat ggaagctata ttgtttgcta tcagtgaaat aattatatgg
     3841 taaacagtgt tctttttctt gtttccatta taaaaatcgt atcgggattt ttcgttcttt
     3901 taaattgaac atttctatct atccgcgtgt tatcaacaaa acaaaatcag gtaaatagtt
     3961 gacgaaacag aaataaatac atagtattat gttttgaagc ctaagtacaa tagtctatag
     4021 aaaaggagcg ataccattgg caaaacgcat tgaccgaatt tatatgtatg tcaaagaaaa
     4081 aaccgctcat ttaacaaagg cagaatatga tcaaggagta acaacacagg aaattgctga
     4141 agtgttaggg attcagcgaa caaatagcag taaagatctg aatcagctag tgcgcgaagg
     4201 aaaactgata aagacagacg gacgaccagt acgttacata tatcaaacac tcgttacgca
     4261 cgtaaagcca gttacaaaac atgtagtcag ttacaaagaa acgccggtct ctgaagaaaa
     4321 aaatacactc caaatagata ctaaggatat ctttgcaaaa atcattggag ctaacggcag
     4381 catgaaaaat tcagtggaac aggcaaaagc tgcgattttg tatccgccaa gaggattaaa
     4441 ttgtttgatt acaggaccta ccggatcagg aaagacttat tttgcacacg cgatgtatca
     4501 ttttgcaaaa gcgcatcatg taatagatga ggaaacggaa ctgattgttt tcaattgtgc
     4561 tgattatgcc aataaccctg agcttttgat gagtcattta tttgggtacg caaaaggtgc
     4621 ttttacaggc gcagatgaag aaaaaatggg gattatcgat caagcggatg gcggtatgct
     4681 gtttttggat gagattcacc ggttgccgcc tgaaggtcaa gaaatgatct tttactttat
     4741 ggatcatgga acttacagtc gtcttgggga aacaacgaaa tcccatgaag caaatgtacg
     4801 gatcgtaggt gcgaccacag aagacccagg ttcttctcta ttagaaactt ttgttcggcg
     4861 tatcccaatc aatatcaaac ttccggcttt tgaaaaacgt cctgcaaatg agaaagtcga
     4921 tttagtaaaa atcatgattg cccacgaagc aaaccgcacg cagcgaaaga tttctttgac
     4981 agaagatgta gcaaaagcat tgatcggcag tgtgacctat ggaaatatag gacaattgaa
     5041 gtcaaatatc cagttagtat gtgcacgagg atttctaaat catatgcaat cacccgaaat
     5101 ctcgatcaca atcgatgatt tgacagaagg gattcgtagc ggattgattc aattagcaag
     5161 caaccgcagt gcaatggcag agctttctaa aatattggag ccaaagataa ctgtagcacc
     5221 aaatgatacg atcatgaaaa tccaatcgga ctcctatgag ttaccttata atctatatga
     5281 tatcatcgga gataaagcag ctttactgaa gtcggatggt ttggatcaag aagcaatcaa
     5341 tcatttcatt tctacagata tcaacatcca tctgaagtct ttctataaag accatggttt
     5401 ttcttttaat gcagacagca aattagcaga atttgtagat ccgaaaatca tagaagtgac
     5461 caatcagatt tatgtaatgg tcaaaagatc tttaccatat gagtttcagc aaaactttat
     5521 ttatgctatg agcttgcata tcagttcttt tttgaaaaaa atcaacttag gagaagaacg
     5581 gcatacgaat gataacatcc gtgagatggc ttttgatttt ccagatgaat atgctgttgc
     5641 gaaagaagtg cgtagttata tcgaaacgta ttttcaagtg gagattccag aaagtgaaga
     5701 ctattacttg accgttctac tcgtctcgtt acgggcaaat caagcaagcg gcagaatcgg
     5761 tgtagtcgtt gcagctcacg gaaacagcac agcaagcagt atggtccaag ttgtccaaca
     5821 gttgttagat gcagagaacg tacgagcagt agacatgcct ttggatatgg atccgaaaac
     5881 agcattggac cgaattgaac gcaatgtaca agaagtagat gaaggaagcg gggtcatact
     5941 cttagtagat atggggtccc ttgcgtcgtt taattcgcaa attcaaagag acactggaat
     6001 tccagtacgt actgttgata tggtaacaac ttctctagtt ttggaaactg tacgaaaggt
     6061 ctcagtatta gggacagatc tagatatgtt gcatgaatca ttgaaaaatt ttcgaggtta
     6121 cgcagaaata tcttctgtag aatctgttca ggaagcaaaa cctattcata agaaaaaagc
     6181 tgttttggct atctgcgcat ctggcgaagg tacagcacaa agaatcaaag aattgatcga
     6241 acgagcggta aacaagcgtc aagaaacaga attagaagtt ctagctttgt cgattgtgga
     6301 agtgaaagaa gaactgccag ttatccaaaa agaatatcag atcatagcta ctactgggat
     6361 tactgatttg aatatccaag ccccatttat tcctttagaa cgttttattg atcaaaatat
     6421 cgaagtcatt ttggatcagt tgctgatgga atcagaattg gaggaaactg aattcatctc
     6481 cctagatgaa gagtcagcta aaaatacgtg tgttgagttt atctctgata acttcatctt
     6541 tattaatggt agtaagttaa tcgatccaat gtggcaattt tctacacaaa tctctcagac
     6601 gacgggaatc ggcgatgaag aatatggatt caaaatcaac ctagtgatgc acacagcagg
     6661 aatgatcgaa cgtattattc ggaatgaacc attgactgtg gaagaaaacg aattgacaaa
     6721 tacaacgaat gatcctcttt atagccaatt ggcagcctca gttgttctgt tggaagatca
     6781 gataaaagta aaagtgccga tagaagaaat gtattatctg ctacgtctag tccataatca
     6841 gttagataaa aaagaataca ctgtgccatg aatacacaaa aacgatacac ttttatagtg
     6901 tatcgttttt gtgtattggt taagaatcct ttaaattcaa ttagtgtatc gtaaaaaagt
     6961 tggcacgctt tttgcttata gtagtgtgtg ctaggaggtg gttgttcaca cagaaatgat
     7021 aggggtttct gtgaagtagt gaggaagaag gagttttgca gatggatatt cgattggcac
     7081 gtatagatga tcgtttaatc cacgggcaag tcgcaacagt ttggtcgaag caaacaggtg
     7141 ttgaacgtat cattgtgatc agcgatgtag tcgctcaaga taaattgaga aagtttctac
     7201 taaaagaagc tgcaccgccg gggatcacag caaatgtaat aaccgtgcag aaaatgattc
     7261 aaatctatca tagtggatta ttatcacgag caaaagtgat gcttttattc accaatcccc
     7321 aagatgttga agcggttgtc cgctgcggtg tacagttgaa gtcgttgaat atcggcggta
     7381 tgagttttac tgaaggcaaa tcaatgatta ccaattttgt ttcagtaaat caacaagata
     7441 ttgattcctt tcgttacttg aacagtcaag aagtggagtt agagattcga aaagtacctg
     7501 ctgacaggaa aatccttttg atggaactac tagcgaaaat caaaaaggtg tagcaagaat
     7561 ggttgttgga aaaggtcaaa taattctgtt gtaatggtta gcaattatta atatttttga
     7621 atttgttaca acaaaagtaa ataggaggta taaatatggt agggattatc ctagcaagtc
     7681 atggacaatt tgctgaagga atcttacaat ccggttcaat gatctttggc gaacaagaaa
     7741 acgtcaaagc cgtgattttg aaaccaagtg aaggtccaga tgacttgaga gccaaattgg
     7801 aagaagcggt tgcttctttt gataatcaag acgaagtgtt attcttagtt gatttatggg
     7861 gcggaacacc gttcaatcaa tcaaacacat tattcgaaga gcacaaagat aaatgggcaa
     7921 tcgtcagcgg tttgaattta ccaatgttga ttgaagccta cgcatcacgc ttttcaatgg
     7981 aatctgctca tgaaattgca gcacacatca ttgaaacagc aaaagacggt gtaaaagtaa
     8041 aaccggaaga attagaaccg gcagaagcac cgaaagcagc agttgaagac gcacaaccta
     8101 aaggagcact tccagaagga actgtggtcg gagacggaaa aatcaaatat gtattagcac
     8161 gtgttgactc acgtctgtta catggacaag ttgcgacagc ttggacaaaa gcagtgcaac
     8221 caaaccgtat tatcgttgtt tcagatgcag tatcaaagga tgatctgcgt aagagactaa
     8281 tcgaacaagc tgctcctcca ggtgtaaaag caaatgttat tcctattagc aaaatgatcg
     8341 aagtagcaaa agaccctcgt tttggaaata ccaaagcatt gcttttattt gaaaatcctg
     8401 aagacgtatt gacagctgtt gaaggtggcg tagacatcaa agaattaaat gttgggtcaa
     8461 tggcgcattc ggtcggtaaa gtcgttgtaa gtaaagtatt gtctatggga caagaagacg
     8521 ttgaagcgtt tgagaagctt gaacaaaaag gtgtgaaatt cgatgtacgt aaagtaccta
     8581 atgattcacg cgataacatg gatgatattt tgaaaaaagc aaaagctgaa ttagcaaaag
     8641 cgtaataaat aatattaaaa tttataggag gcttatcatg tctattattt caataatttt
     8701 agtcgtatta gttgcctttc tagctggtat ggaaggtatt cttgatgaat ttcaatttca
     8761 tcaaccacta gtggcatgta cattgatcgg tttagttacc ggtaacctcg aagctggtat
     8821 cgtacttggc ggtacgcttc agatgatcgc tttaggttgg gcaaatatcg gtgccgctgt
     8881 agcaccagat gctgcattag catcagtagc atcagcaatt attttagttt taggtggaca
     8941 aggtgttaaa ggtgttcctt ctgcgatcgc gattgccgtt cctttggctg ttgcagggtt
     9001 gttcttaaca atgatcgtac gtacaatcgc tgttccaatc gttcatttga tggatgcggc
     9061 tgctgaagaa ggaaacatcc gtaaggttga aatgtggcac atcattgctg tatgtttaca
     9121 aggggttcgt atcgcggtcc cagcagcagc gttgctattt atcccagcac aaactgtgca
     9181 atcattcctt gaatcaatgc cagcatggtt aacagatggt atggctatcg gtgggggaat
     9241 ggtcgtagct gtaggttacg ctttggtaat caacatgatg gctacaaaag aagtatggcc
     9301 attcttcgtt attggtttcg ttgtagctgc aatttctcaa ttaacattga ttgctctagg
     9361 tgctttaggt gttgcattag ctcttattta cttgaacctt tctaaaatgg gtggttcatc
     9421 aaataatgga ggcggaagca actctggtga ccctttaggc gacattttga atgactatta
     9481 atcttgaagg aggaaaataa aatggcagaa gaaaaaatca aattatcaaa aaaagatcgt
     9541 ttagctgttg cgtggcgctc taccttcatt caaggttcat ggaactacga acgtatgcaa
     9601 aacggtggct gggcattttc tatgattcca gcaatcaaaa aattatacaa aacaaaagaa
     9661 gaacgttctg cagcattaaa acgtcacttg gaattcttta atacacaccc atacattgct
     9721 tctccaattt taggggttac attggctctg gaagaagaac gtgcgaatgg cgcgcctgtt
     9781 gatgatgtag cgattcaagg ggttaaagta ggtatgatgg gtcctttagc tggtgttggt
     9841 gacccagtgt tctggtttac agtacgtcca atgctaggtg cactaggtgc ttcattagct
     9901 atgggaggaa atatccttgg accaatcatc ttcttcttag gttggaacat tattcgttgg
     9961 gcattcatgt ggtatacaca agaatttggc tacaaagctg gttctaaaat tactgatgac
    10021 ctttctggcg gattgttaca agacgtaaca aaaggtgctt caattttagg gatgttcgta
    10081 ttagctgccc ttgtacaaag atgggtatca atcaaattct taccagttgt atcaactgta
    10141 aaattagata aaggtgctta catcgaatgg gataaattgc cttcaggtgg tgaaggaatc
    10201 aagagtgcat ttgaacaagt taataatggc ttagcccttt ctcctgaaaa ggtaacaaca
    10261 ttacaagaca acttagacca attgattcct ggtttagctg cattaggatt aacattgtta
    10321 tgtatgtggt tgcttaagaa aaaggtttct ccaatcgtta tcatccttgg tttattcgta
    10381 gtcggtgttg ttggacacgt catcggactt ttataatcca taaaaatata gctcgggcgt
    10441 tgtcccgagc tttttttata tagtatactg ttcataaaca aatatctgga gggagaaaat
    10501 ggttcaatca atcaatacaa aagtagactt ggtaattgat gctacttcgt ttacgggtct
    10561 gacagattac gggcaaatca tgataggtga taatggtttt gagttttacc atgcgcgaga
    10621 tagacgcaaa tatatccaaa ttccatggga agaagtggat tatgtactag catcagtcat
    10681 gctaaaggga aaatggattc ctcgatatgc cattcaaacg aaaaaaaatg gtacatacac
    10741 attttcttcc aaagaaccta aaaaagtgct tcgtgcgatc agagaatatg tagatgaaga
    10801 tagaatggta caatcattga gtttctttga tgtagtgaaa cgtggtattc gtagtatgtt
    10861 taaacgcaag taaaaaataa agaaaacttc taagaggagg ttttctttat ttttttactt
    10921 tgcagaagaa aatatgaaat agcaaaacca attatttgtc ccctttaaat cccatatgct
    10981 aaaattgatt agcaaagaag gaggttatat tatgagttgg ttatggacat tgattgttgg
    11041 agccgtgatc ggagcaattg caggtgcgat caccagtaaa ggaaattcga tgggctgtgt
    11101 aacgaatatc attgctggac tagtcggttc aagtattgga caagcgattt tcggcgaatg
    11161 gggaccaaaa gctgcaggta tggcacttat tccatctatt ttaggcgcag tgattttggt
    11221 agcagttgtc tcatttttct ttggaaaacg atcataagtt tttacaaaat agtaaactaa
    11281 aaggagttgc agcgagactg tgactccttt ttggctttat ttatttaaaa taaaaagaag
    11341 ttttccggaa atttttattt aactgtcgtg tacgaagaaa agtttgctat aatggaaaaa
    11401 agcgtgcaaa gaaagaggga gtttcattat gatgctacgt gtagaaaaat tgcgcaaaaa
    11461 aatgcaagaa gaaaatttgg attcattttt ggttaccagt ccgtacaatc ttcgttactt
    11521 aacgaacttt acaggtacga caggattagc tgttattact ttggaaaaag cattttttat
    11581 tactgatttt cgttataccg agcaggcggc agcgcaggct caagggtttg aaattattaa
    11641 aaatgtcgga cctatttttg atgaggtagc aaacttagta cgcaaagaag aactgcactc
    11701 attagctttt gaggaaacaa cagtctcatt tttagaatat tcggttttag aagaaatcat
    11761 cgatgcggaa ttagtacctg tttcacagat gatcgaagaa ctcagagaag tgaaagatga
    11821 agaagaaatc gctattatcg aaaaagcatg tcatattgcg gatatggcat atgatcacat
    11881 cctgaaaatg atccaaccgg gcatgactga gattgaagtc gcaaatcaac tagactttta
    11941 tatgcgttct cttggcgctt caggtgtttc ttttgatacg atcgttgcta gcggattgcg
    12001 ttcggctatg ccgcatggtg tagcaagcaa aaaaatcatc gaacaaggtg acttgatcac
    12061 attggacttt ggttgctatt acgaagggta cgtttccgat atgacacgta cattcgctat
    12121 tggtgatcct ggggaaaaat taaaagaaat ttatcaaata gtcttagatg ctcaattagc
    12181 agttattgat gctgcaaaac caggaatgac tggtgtccaa ttagatgcag ttgctagaga
    12241 ttatatttca aaacatggtt acggggaagc atttggacat tctacaggac atgggatcgg
    12301 tttagagatc cacgaaggac caaacgtttc ttctcgtgca gaaaagcaat ttgtaccagg
    12361 aaatgtgatc actgatgaac cagggatcta cttaccagga atcggtggag tacgtattga
    12421 ggatgacttg ttgatcacag aagatggaaa tcgtgtgttg actcgttcac caaaagaatt
    12481 gatcatctta tagtttcatc aatagaagaa gtaagcgtaa aaatcagtgt tcacagactt
    12541 tgttatagaa aatcactcga tttaatgata cactatagaa gaacttttaa gctgtaagag
    12601 ataataaagt tgggtgtttt ctccaattca tagcagtttg cagctgatcg gcggcagttt
    12661 tcttaccacg tgatcactat gccgcatcat agagaattcg gatgtatctc gtgcttaggc
    12721 aaagggagga ataaataaat ggcagatgaa aaaaatttga ttttaaacgc aaaccaagat
    12781 ttaggagaga tcgtgatcgc tcctgaagtc atcgaagtaa ttatcgggat tgcagcttct
    12841 aaagtagaag gcgtatacgg aatgcgcgga acttttgcca gcaatgtcac tgaattatta
    12901 ggacgtgcag ctcacggaaa aggtgtttac ttagcaaacg aggaagatgg cttgaaagta
    12961 gatctttact gttatctaga atatggtgtt tcagttccta aagtagcaat ggaaatgcag
    13021 gaacgggtca gccagcaagt ccttttcatg acggatatcg atttatctga agtcaatatc
    13081 catgtagtag ctgttgttcc tgaaaaatta gaacaaccaa caatcgaaga actttttgaa
    13141 gacgaagagg aagagaatga ataaagaatt atcaagacac gagattcggg aaatggcatt
    13201 gcaggcttta tttccattag acttcaatgc agacctaaca aaagaagatg cgattttcaa
    13261 cgcaatcgaa ttagaccacc gcgacatgat caacgaagat gaatcagagt ttgttcctgt
    13321 ttatcttgat acgctagttg gcggtgtctg tgcaaaaaaa gatgaattgg ataaagtgat
    13381 tgaaaaacat ttaaaaaaca attggcacat caatcggatc tcaaaaatgg atttggtgat
    13441 cttgcgtatc gcgatttttg aaatgactta tgtgacagat gtaccagcgg ctgttgcttt
    13501 aaatgaagca atcgagttgg caaaaacatt tagtgatgac cgttcacgta aattcgtcaa
    13561 cggtgtttta tccaatgtat tgaaagaatt agaagctggg gcgtaagtcc tagctttttt
    13621 ctatgaatca tcataaacct gttataaatc gggaaaattc tatggtagaa tgaaaacaca
    13681 aaaaatatct gggagtggtt agatggcaga attgattaac ggaaaagaat tagctgaaaa
    13741 aatgcaggca gagattgctg aaaaaatcca aaaattgaaa gataatggta tccatcccgg
    13801 attagtcgtt ttattggtag gggaaaatcc agccagccaa atctatgtaa gaaataaaga
    13861 acgtgtagca aaagaaatcg gaatccattc attggtagag cgttatcctg agacgatttc
    13921 tgaagacgaa ctacttgcag aaattgaaaa atacaatcaa gatcctagtt ttcatggtat
    13981 attggtccaa ttaccattgc caaagcatat agatgaagaa aagatcctgt tagctatccg
    14041 ccctgaaaaa gacgtagacg gttttcatcc gatgaattta ggcagactcc ttgcgggtaa
    14101 accggacaag attccttgta ctccatacgg catcatgaaa atgtttgaag cttattctat
    14161 cgatccggcg ggtaaacgag cagtagttat cggacgaagc aatatcgtcg gtaaaccgat
    14221 ggctcagcta ctcttgatgg cagatgcaac tgttactgtt gctcattcta aaaccgaaaa
    14281 tctggctgaa ctggcacaag aagcagatat tttagtcgta gcaataggac gtggacattt
    14341 tgtcacaaaa gaattcgtaa aaccaggtgc tgtagtgata gatgtaggga tgaatcgtga
    14401 tgctaacggt aagctgatcg gtgatgtgaa atttgatgaa gtggagccta ttgcaggctg
    14461 gatcactcct gtgccaaagg gtgtagggcc aatgactatc acgatgctga tgtatcaaac
    14521 cgtaaaatct gccgaagctt ttggagcagg tgaatagatg gcacaagaat atttaactgt
    14581 cactacatta acgaagtatt tgaaaagaaa atttgatgcc gatccttatt tagaacgcgt
    14641 atatttaaca ggcgaaatat cgaatttccg tttacgtgcc aatgcccatc aatatttcag
    14701 cttaaaagat gaccatgcaa aaatatctgc tattatgttc aaaggagcat tccaaaaatt
    14761 acgattccag ccaaaagaag gaatgaaagt ccttgtcgtt gggcgtattt cattatatga
    14821 agcgggaggt tcttatcaaa tctatgtaga gcacatggag cctgatggaa taggtgcttt
    14881 atatcaagaa ttggaagaac gaaaagaaaa gctgatgaag gaaggccttt ttcaagggcc
    14941 taaaaaaatg ctgccacgtt tcccaaagcg aatcgcggtc cttaccagtc caagcggagc
    15001 ggttatccgc gatatcatca cgacggtgaa aagaagatat ccaattgtcc aattggtttt
    15061 gttcccaaca gttgtacaag gaaaccaagc agcggacaat gttgtcagga atatccagag
    15121 agtagaagag atgggcaatt tcgatacaat gatcatcggt cgtgggggtg gttcgattga
    15181 ggatttatgg ccgtttaatg aagagcgggt tgctcgggcg atttatcaag ctaatacgcc
    15241 ggtgatttca tctgtaggac atgaaacaga taccacgatc gctgatttag tagcagatgt
    15301 tcgggctgcc actccaacag cagctgcaga attagctgtt cctgttttgt cagaagagtt
    15361 gatgcgcatt caagaacggc aagctagatt gcagcaggcg tttgtacgcc agatccagcg
    15421 caaacaagaa cggttcgagc gtgctcgacg ttcctatgta tttcggcagc ctgaaagatt
    15481 gtatgaaggt cagaccatca aattagacca gttgaaacag cgattatatc aagctacaca
    15541 gcaaatttat catgcgaaag aaaaacaaac gatcgcatta agccaccaat tggaacaagt
    15601 ggctccgctt tatcgagtta aagcagctcg ccaagaaact gcttatctag aaaaacgctt
    15661 agaagagaaa atccaacaat atatgaaaca acagcagcag cgttttgctc aagctgttca
    15721 atctcttgat ttgttgagtc cattgaaaat catgggaaga ggctacagct atacgacgtt
    15781 aaatgatcaa gtagtaaaaa gtgtcaccga gattcatcaa ggggatttgc tgacgatcca
    15841 ttatcaagat ggaatcgtta aaggacaagt acaagagata gacaaaggag aataaaaata
    15901 tggaaaatcc aacttttgaa gagtcactgc aagaattaga aaaaattgtc atgcagttag
    15961 aacaaggaga cgttccatta gaatcagctc tagattcgtt taaacgagga atggaattaa
    16021 gcaaacattg ccaagataca ttagctaaag cagaagagac cttgacgaaa atgatgacag
    16081 aatcaaatga agaagttgat tttgacggaa acgaggaaag ctgagatgag attaactgac
    16141 ttttcagcta ttcatctacc tgctgtagaa aaagaaatct tgtctttttt agatgagcat
    16201 acaacggata gaggactcta tgatgcgatg agttattcag tgaaagcagg cgggaaaaga
    16261 atacgtccct tattactttt aacggccgtt gcttcctttg atgagcctat tgatgttccc
    16321 gtctatcaag tagcagctgc tttagaaatg gtgcatacgt attccttgat ccacgatgat
    16381 ttgcctgcga tggacaatga tgatcttcgt agaggaaaac ctaccaatca taaagtattc
    16441 ggcgaagcat tagctatatt ggccggagat ggtttgctta caggagcatt ccagctaatc
    16501 agtatggcac acttaggtaa ttctcctaag cttctgctgc ttcagcaatt agctgtttgt
    16561 gcgggatcac aaggtatggt tgctgggcaa gctgcagata tcgagggcga atcgaaaaag
    16621 ctttctttag aagaattagc atttattcat gaaagaaaga cagggcattt gattcgttat
    16681 gccttgctgg ctggtggcat attagcgaag caacctgaag aaatcttatt gctacttcaa
    16741 cgattagccg aacatttagg tttagctttt cagataagag atgatctact cgatgtgatt
    16801 ggtacaacga aaacattagg gaaaacagct ggtaaagatg aacgtatgga aaaaaatact
    16861 tacccgcgtt tattaggcct agaaaagaca cgtgaggcgt tggagattga gctgttatcc
    16921 gccaacaaga tcatagacaa attagaagag gacgtttttt cttttgatgg cgggctgatg
    16981 cgccagatga tcaagcaatt tgatgtggag aaatgaggga aagaatggaa aaagaacgcg
    17041 tagatgtatt agcggtcaaa caaggattat ttgaaacaag agaacaagcc aaacgttcag
    17101 tgatggctgg tttgatttac aacgaaaaaa acgagcggtt tgataaaccg ggagaaaaaa
    17161 ttccagtgag tagtgaatta agagtcaaag gtaaaaaact tccttatgtt tctcgagggg
    17221 gattaaaatt agaaaaagca ctcaaagcat ttgatctatc tgttgaagga aaaacattgt
    17281 tagatattgg tgcatccact ggcggattta ctgatgcagc tttgcaaaat ggcgcaaaaa
    17341 tgagttatgc tttagacgtt ggctacaatc agctagcttg gaaaataaga caagatccta
    17401 gagtcgtcgt aatggaaaga gtgaattttc gttatgcaaa acccgaagac tttaccttag
    17461 gaattccaga agttgctgtt atcgatgttt cttttatttc actaaaactg atgctgcctc
    17521 cattacatgc tattttaaaa gaagagggag aagtgattgc tttaatcaaa ccccaattcg
    17581 aagcaggcag agaagctgta gggaaaaacg gaattgttcg tgaccctcag acccatcaaa
    17641 aagttttaga agacattttg tcatttgcgg cacaaggtgg atatgatgtg ctggaattaa
    17701 gttattctcc gatcacgggg ggagaaggaa atatcgagtt cttggctcac ttgaggaaag
    17761 tacccgaaag cggaacaatc aattcagcta ttaatatggc agaagtggct tcaaatgcac
    17821 acgaacagtt tgaccataaa taaattgtaa ctgtgaaaat caagtgagaa gtatgcggat
    17881 tcttctttat tataccaagg aaataagcta tcattgatta agaagaatat gctaaggagc
    17941 gaagacgtaa tgagaaaaaa agatcggcat cgtttgatta ctcgattgtt gagtgagcaa
    18001 gatatccgaa agcaggaaga atttgttgaa atcctgaaaa gcaaaggaat atctgttact
    18061 caagctacga tttccagaga tataaaggaa ctgaaactga tcaaggtacc ggcttcagat
    18121 ggcggctacc gttatagcat accagctgaa acaaacgaag atgtaaatac taaattggaa
    18181 aaactattaa aagacgcttt cgtggcagtt gaccaaatgg aaaaatttgt tattttgaaa
    18241 acattgccgg gaaatgcttc ggcagcagca aatttgattg ataaacgtta caagaaagaa
    18301 ttattttcga ttatcaatga tgacgataat gttttgatga ttacacgaac tgaagaccag
    18361 gcagttttat tgaagaaaga cttcctgcat tacctttaag ttgaggagcg aatgtgatgc
    18421 tacaagaaat cagtattact aactttgcga ttattccgga attgcggctt tcttttcacg
    18481 aaggaatgac cgcgctgacc ggagagactg gtgcgggtaa atccattatt atcgatgctt
    18541 taggtctgtt agcaggagga agaggttcaa gcgattatat acgtcaagga gcagaaaaat
    18601 gtgtgcttga agggttgttt gaactaccaa aacaagaagg attctctgaa ctgatggttg
    18661 aattagggat cgaaacagat gaggacaatc tgattgttcg acgggatatg tccttaactg
    18721 gaaaaaatgt ttgtcgagtg aatggtcata ttatcacatt agctaatctg agaagaatcg
    18781 gcagttattt agtagatatc caaggacaaa acgaacatca ggaactgctc cagcctgaat
    18841 cacatcttgc tttattggat cgttttggag atgtagcttt ccaacagaag aaaaagagtt
    18901 atcagcaaga atatatctct tatcgtgagc tggaaaaaag ggtacgaaaa gtgcagcaaa
    18961 atgaaaaatc ctatgttcag agaatagata tgctccattt ccagcaagaa gaaatagctg
    19021 ctgctgattt gcaagtcgga gaagaagaga aattaaaaga agaaagagaa aaacttagca
    19081 attatcaaaa aattgctgat ggattagctg caggttacgg agcactgacg aataatgaac
    19141 aaaacagtgt agatggtgtt ggcttggccg tttcagaact gcaagggatc gcccatctag
    19201 atgtggagta tgaagcaatt tatgaaaata tccaaagtgc ttactatttg ctgcaagatg
    19261 caatcggcga tatgagtcgt cagattgatt tattggagtt agatgagagt cgtttagaag
    19321 aagttacgca acgtttagaa ttaatccgac agttgaaacg taaatacggc gaatccattg
    19381 agtcaatatt ggcttactat gacgaaatca ccgaagagct agcttcttct gatttttcag
    19441 aaggtcaatt ggacaaaatg aaagaggaac tagagcaaaa agagcttcta cttcagcaac
    19501 aagccgcaga tcttcatgaa gcgcgtaaaa aaatcgcaaa agaattggaa aaatccattc
    19561 tgcatgaatt gaaaagtctg tatatggaaa acactgagtt tgaggtacgt ttcttaaaag
    19621 aagaaaatag acaattgaat catgatggat ttgaccaaat cgaattttac attacgacca
    19681 atccaggaga gccactgaag cctttggtga aagtcgcttc tggtggcgaa ctgtctcgga
    19741 tgttgcttgc tttgaaaact attttttctt ctgagcaagg tgtgacaagt attatttttg
    19801 atgaagtgga tacaggtgtg agcggaagag ttgcacaggc catcgcagat aaaatttctc
    19861 aaatatcgaa atactctcaa gttttgtgca tcactcatct gcctcaagtt gctgcagtcg
    19921 ccgactatca atattatatt gtgaaagcag tgattgatgg acgtacgcaa acttctgttt
    19981 cagagttgaa aacaaaagaa agagaagaag aaattgcacg tatgctggct ggaagtgaga
    20041 ttacaaaact cacactcgaa catgcaaaag aactattgaa actcgcaaaa aggacctgag
    20101 ccacctcgca gtggttcagg tccttttcag cagctgatag ctatatttta tttttacatg
    20161 acactcatta cagcagtcaa tacaacagat ccaatcgtta aaatcagcat tgtccaaatg
    20221 acaaattttg ttactttagc aaaagtactt gtttgttttt tttcacgcat tgttttcaac
    20281 ctcgtcatta attagttgat tcaagtgtac aatctttttt ccatttccac aagagggctt
    20341 ttttataaaa acttcccttt ttttaaataa taggtagtta cgatacaaat aaataatgtt
    20401 aatagaacta gccatataaa ggcatcatcc ctttcagcaa taggtaactt aatgttcatt
    20461 ccatagatac tgccgacgat tgtcggtata gttaaaataa tcgttaaaga agttaaaatt
    20521 ttcataatat tattcagatt attcgatacg attgctgaaa aagtctgatt catttgttct
    20581 actagtttta attgaatgcg agaagtagtc attgcctgct ctgtttccac caatacatca
    20641 cgtaaatgat ttttcaaagc atgattttct tggaaaagtt tattttgcgc tagttctttc
    20701 aatgcatcta aattagcaga agtcgcttct ctgaaataaa cgaggctttt ttggatatcc
    20761 atgatttgat aaagttgttt gttttcagtg gatacttgaa gtttggcttc caaagtatcg
    20821 cattgagatt gaagcgtagc taaataactg ttgtaagaga gtgtgacctg ccaaatgaat
    20881 tgaaagaaga tattgagatt aggcgaaata tcactttttg gaaaggtctg gctaaagact
    20941 ttctttaaaa acagtggttc atgattcgta attgtgataa gcttcttttt gggcgtcaaa
    21001 ataatagaga gcgggtatgt atccacttga aaataaccgc taggacttgt tttagcaaaa
    21061 ggatattgta acaataataa cgctggcttt gtcaactttg attgattgaa ttcttctgta
    21121 cgggaatttt ctgcatcgtc caatatggaa gtgatgtaat ctgacggaat ttcaaattga
    21181 tgtgtcagct gatgaatctc ttcttgatta ggacgttcta cacaatacca gatcgtttgg
    21241 tcagtttcct ttccggtatt acgcagtttc tcattatcta atgtaaaata tttgatcatg
    21301 attttcctcc tgttgcctgt ttcttttatt ataacggata gggaattgga atacatggaa
    21361 tgtgtttgta agatatagaa tataactgca taaatttaaa taaaatgaaa cagttgtgta
    21421 aagtatatac agtatacttg aagcaacgat aaaaaaggag tttacgttca tgggcacatt
    21481 cacaccactt atcttgtttt ggtatattta cccggtaatc gttttgtttg gttgccaatt
    21541 tcttgtaaaa ttattttcgt taaatcgtcg ttttaaaata aaagcccctg atttggcgat
    21601 tccttttttg tggggcggta tgcatgcatt gtctagaaat acagggacaa tttctatctt
    21661 accgtttttc tttatttctg ttttgctaat gggaatttgt attgctgttt tccaagcata
    21721 ttattatgaa gaaattattt atccgcgtta tttcaagatg acttggcgtt tggtttttct
    21781 gttgacaatc gtcttgtacg ctgtcttgat cattgtgaac atactttctt acctatgaaa
    21841 aaaatagaaa cctgaataat tcataccgat aaaaaaatga tctgttgctc taaattttac
    21901 cggaatttat attttttatt ccgtctcaga acccaacaga ttaaaaaatc cgaactgttt
    21961 aggtaaacag ctgcggttca gccaaatttg ggcgcactcg tcccttgaag tacaatataa
    22021 tttttaaaat tgggatttat ttaccagcag agctgctgga tatttccgag gacttgataa
    22081 aataagccct gatgtaaatg ataagaactg agtttcagca gcagtctacg tcctgttcga
    22141 acgagtttac ctgcgacctt gaatagtttt aatcggattg tgtccacctg catgctggcc
    22201 gatccctgtg tgaaacaaag cgtacgcata aagttaacga tgttgtaagc caacaggctt
    22261 accatcatgc gagcgtgatt ttctaaaaag gaagggctat cggtcttatc gaaataaaag
    22321 ccattcttcg cttctttaat gaagttttcc atggtgccgc gtttacagta agtccgaaag
    22381 actgtttccg cagagacatt attggagtaa ttggtgacga tgtattcatg tcggaataga
    22441 agttcatcag cttcccgtgt tgatttaatg cagactcggc gttctttgga ccaacttttt
    22501 gcttgatagg atgttgaaga ataaacaact tcttttttat cccaagggtg gttatcgtca
    22561 atctgaataa agctttccgc aatttttgaa aggttacggt tcgcctttaa gcggatcaca
    22621 aagaagctgt cataagcttc acagagatcg taaagttccg gtgtagcgaa cccgctatct
    22681 ccacggacga gaatattgct gacaggaacc acttggttat aatgctcaaa aagaggctcc
    22741 ataaatgtcc caacgccatt agacgtgtag acattgccag aacgaagttc tgcttttaag
    22801 aaatctcccg tcaaaccatc aaatgcgact aacggatggt atccattggt tcgataatga
    22861 gcattgtaat cagtgttttc ttgatctccg taagtatcag aatgggttga atccaagtca
    22921 aagatcattt ctgtcgtatt tcttgccgac cgtactttat caatcatggc ttggttaaga
    22981 tcttgcaact gagaaatatt ttcttcgctt atgcgatccc agaatcggga aatcgaagac
    23041 tgcgaggcaa gcctggactt atccaaaatc accttgaata tagggtcttg cttcaatata
    23101 tttgcggagg aatcggtcga atagccagcg atattttgat aaatcaactg ctccatcaaa
    23161 gaaaaattgt catggatatg atagagacgt ttgtcctcta ttcccaagta ttgcttagaa
    23221 agatcggaga aattaagaga ctccataaat tctttgacga gaaccaatcc agaatcggtg
    23281 gacaaatttc cacctgtgtt tgaaatcgtc agtttagagt tgaattgtac ctttttttcg
    23341 tgtaatgtag ccattgagag agcccctttc tatggtttta gtgtggtaac ttaaccatat
    23401 cagattgggg ttctttttgt acacccaaag ggtgcttatc gaacaagtga aaaatgctat
    23461 tgcagcaagg ttatttgtag tttcctgaaa ttttatgaat tattcaggta gaaaagctgt
    23521 aaaaagtctt ttaaaaggct ttttgcagct tgatttttaa aaaaatgttt atgaataaaa
    23581 aaagttgttc ccggatttcc cagaaaaggt ggtagaaagt gggggattgt ggtagactaa
    23641 ttttagttag tggaggaatg gggggctttc aatgttgatg ggtgaattcc aacataatat
    23701 tgatgcaaaa ggacgtttaa tcgtgccctc aaaacttcgt gaagaacttg gcgaaaagtt
    23761 tgttctgaca cgcggattgg atggctgtct tttcggctat ccgatgagcg aatgggaaaa
    23821 tctggaagct aaactgaacg aaatgccgtt ggctaaaaaa gacgcacgta cttttgtgcg
    23881 attcttctat tcggctgcca ctgaatgcga acttgataaa caaggacgaa tcaatatacc
    23941 cagcacgtta agaaattatg cagctttgac aaaaggttgc gtagtcatcg gtgtatctaa
    24001 ccgaattgaa atttgggatg agacacgatg gcaagatttt tctgcagcag cagaagagaa
    24061 ctttgacgaa attgctgaaa gtatgattga tttcggacta taagaatagg aagaggctga
    24121 ctaatgactg aaacatttca acactataca gtcatgttga aagaaacggt agatggattg
    24181 aacatcaaac cggatggcgt ttatgtagac tgtacactcg gcggtgccgg acatagtgaa
    24241 tatttattaa gccaactaag ttctaaaggg catctgtatg cgtttgacca agatcaaaaa
    24301 gcaatcgatc acgccaagct gcgtttagct tcttatgtcg aacaaggtat ggtcacattt
    24361 atcaaagcga acttcagaga attagaagaa cgcttgagtg agcatgtaga taaggtagac
    24421 ggcattttgt atgatctagg ggtttcctct ccccagctag atgaagcaga acgtggattt
    24481 agttatcatc aagatgcacc gttggatatg cgcatggatc agtcagctcc tttatccgct
    24541 tatcacgtaa ttaatgaata ttcctaccat gaattagtaa agattttctt ccgctatggc
    24601 gaggagaaat tttccaaaca aattgctcgt gaaatcgaac gagtacgaaa aacaaaacca
    24661 atccaaacaa ctggtgagct tgtcgaaatc attaaacaag tcatcccagc tcccgcaaga
    24721 agaaaaggcg ggcatcctgc aaaaagaatt tttcaagctg tgcggattgc agtaaatgac
    24781 gaactcggtg ccgaagaggc ctctttagaa caggcaatcc ggttattgaa agtcaatgga
    24841 agaatcagcg taatcacatt ccattcattg gaagacagaa ttgtcaaaag tatgtttaaa
    24901 gaatatagta cggtacaaga tctaccaccg ggacttcctg tcatacctga cgaattccaa
    24961 ccagaactga aagtcattac tcgtaaacca atccttcctt cagaggaaga gctaacagaa
    25021 aacaatcgat cacgcagtgc aaagttacgt attgcagaaa aaataagaat gaaagaggag
    25081 tagaatgatg gcagaattaa agaaaatgca agattacgaa tatgatttac ctgaattggt
    25141 ggatcagccg gaacaacaaa tgcctgattc ttcttcgaat caagaaatca ttcttccaca
    25201 atcaccggca aaacgattaa agcagatttc tgctttggaa aaaattgctg tggcaagcgt
    25261 ggcgattgct attgttgctt tatgtgtttt aacagttatg ctgcgcacaa atatcagcgg
    25321 agctgaaaaa gaaatcacgt caatacaaac agaaatcaat gataaaaagc aagaaaaaac
    25381 gagtttagaa caagaaaaaa acgaactttc tcgtactgaa cggattaaac aaatcgcaga
    25441 agaaaaagga ttgtctatta atgacgacaa tttaaggaaa gtgaagtaaa tgagtcttaa
    25501 aaataaaatt cggcggttta tggaaaagaa aaatctaaat ccgatgaaca atcgcaaaaa
    25561 agtaggtatc attttatttg ctacgagtat tggattgttc tttttatttg ccgtccgttt
    25621 ctcgtatatc gttattggag gtcatgttgc tggtacctca ttagctgaaa aaacaaaaca
    25681 gttgtaccaa ggaagtgaag tagtaaaagc aaaaagagga acgatttatg atcgtaacgg
    25741 tgtggcgctc gcagaagatg cctcctctta ttcaattaaa gcgatactct caaaaacata
    25801 tacctctggg gataaaaaac tgtatgttga agaaaagaat tttgataaaa tagcagaaat
    25861 cctgcataag aatctttcta tagacaaaaa agatgcactg aatattttag aggacggcgc
    25921 aaaaaaagag ctgtatcaag ttgaatttgg tagctatggg aaaaatatct cccaagaaac
    25981 caagcaaaac atcgaagcgg acatgaaaaa agaaggtgtc gctggtcttt attttgtcga
    26041 ccatcaggct cggatgtatc caaatggcgt cttttcttct cattttattg gatacgcagt
    26101 tccagacaaa gatgagaatg gcttagttgg taaactaggt ttggagtctg cttacaatga
    26161 tatcttgagc ggtaaagatg gtaagattat ttatcaaaaa gataattttc aaaatcctct
    26221 gccaggtaca gtggcagaag aagaaaaagc tgtcgacgga caagatatct acacgacttt
    26281 agacagcaga ctacaaagtt atttagaaac actgatggat caagtaaatg aagaatatca
    26341 gccagaagaa ttgacagcag tcctgatgaa agcaaaaacc ggagaaatct tggcaatggg
    26401 acaacgacct actttcaatc ctgaaacaat ggaaggattg actggaaaag atgctatctg
    26461 gcgtaatttc ttggtacaag atagttatga accaggatca accatgaaag ttttcactac
    26521 tgcagctgct atcgaagaag gagaattcaa cgagaatgaa accttccaat ctggaaaaat
    26581 ccaagtagaa gatgctacga tcaatgacca tgactttggt gaaaaaggtg tgctgaccat
    26641 gcgtcaagct ctttcatggt caagtaacgt cgggatggtc atactggaac agcgattagg
    26701 cggacgatgg tataactatt tacaaaaact gggatttgga caaagtactc attctggatt
    26761 agatgatgaa gtaaacggtg ctttacctac attaaatatt gtcgaccgag caatgagtgc
    26821 ttatggacaa gcggtaggtg tgacgaattt ccaaatgatg aaagctttca cttctattgc
    26881 gaataacggt acaatgattc aacctcgata tatcagcaaa gtggtggatc cccaaactgg
    26941 agaagagcga acaactcaaa cagaagttct agggcaacct ttttcaaaag aaacaacaga
    27001 aaaagtccgt gaatatatga gagatgttgt cgaaagcgaa aactacggta ttgcttatgg
    27061 tgtatatagt gtacctggat acaatgtatc ggcaaaaacc ggaacagccc aaatcgcttc
    27121 tgatacaggg ggatatcaaa caggagatac agcctacctg tattcgattg tggagatggt
    27181 tccttctgaa gatccagact atgtcttgta tctaacaatg aaacacccta agacatatga
    27241 tcgaatggca ttggctaaaa tcgcaaatcc attgatgaaa cgggcaatgg attttaaaga
    27301 aactgaagaa gattcagata cagaaacaaa aacagaaaaa gtatccgtag ccgattatcg
    27361 taatcttgaa gcggacgttg cagcagctga tgctcaaaaa agtggactgc aacctgtagt
    27421 gatcggtaat ggtaaaaaag tacaaaaaca atctactgcc aacggtgatc agttgatctc
    27481 tggagaaaaa ctgatcctat atactggtgg tgataagctg atgcctgacg taacaggatg
    27541 gtcaaaagca gatatcatga agctggggaa aatcctcggt attgaagtta gttttgatgg
    27601 agatggttat tgtgtcaaac aagaacttgc tccatatgaa aaaatcactg aagacaaact
    27661 tagttttact ttagaagaat aagaagagaa ataggagaga aaagagcatg gaatggacac
    27721 aagcattgat tcccattgtc agcagttgtg caatgacaat tgccgcaatg ccgctattta
    27781 ttggttattt tcaaatgaaa aagcaaggtc aggcgattcg tgaggaggga ccaaagtggc
    27841 acaatagcaa agccggaact cctacaatgg gcggcttagt gtttctgatc ggctctattt
    27901 tgacaggaat ttgggttgga gcttggcaaa aacagctaac tcctactctt ttcatcttgc
    27961 tttttgtatt agcgttatac ggagtaatcg gctttcttga tgattttata aaaatattca
    28021 agaaaagaaa catgggactg aactcgaagc agaaattgat tggacagatt ctagggggaa
    28081 tcatctttta tcttgtttac cgttctgaag gttatccagg tgtattgaat ttcttcggac
    28141 tagatctacc tttaggaatc atttatggta ttttcgctct cttctggctt gttggatttt
    28201 caaatgcagt aaatctgaca gatggcatcg atggattagt tgctggctta ggaagtatct
    28261 cttttgcagc ttacggtcta attgcttggc atcagcatca gtatgatgta ttaatcattt
    28321 gcttaagtgt tttaggaggt ttactgggtt tcttcgtcta caacagaaaa cctgccaaga
    28381 tttttatggg agatgtcggt tcattagcgt tgggcggact tttagcggca atttctataa
    28441 tgctaaacca agaatggaca ttgcttttga ttggtttgat ctatgttatg gaaacagcaa
    28501 gtgtgatgct tcaagttact tcctttaagc ttacaggaaa acgaattttt aaaatgtctc
    28561 caattcatca tcactttgaa atgtgcggat ggtcggagtg gaagattgat acagtttttt
    28621 ggttgatcag cattgtgact tcgttgatca cattatggat cgtttggtag gagatgacgg
    28681 gaaatgaaaa aaatatcaac atatgaaaat aagaaagtgc ttgtcttagg actcgcaaaa
    28741 agcggagtaa gtgcggccaa attattacat gaattgggcg cacttgtcac cgttaatgat
    28801 ggaaaacctt ttgacgaaaa tccggaagca caagagctgc tttctttagg gataaaagta
    28861 ataacaggta gtcatccaat cgaattgttg gatgaagaat tttcattgat ggtcaaaaat
    28921 ccagggattc cctacagtca tcctttagta gcaaaagccc aagaaatggg tattccggta
    28981 attacggaag ttgagctagc ttacgaagta gcggaatgcc caattattgg gattacagga
    29041 acgaacggaa aaacaacgac aacaacgatg acaggactac tactgaatgc aggagcagat
    29101 caaggaattg cacgtttagc gggtaatatt gggtatccag caagtggtgt agcgcaagaa
    29161 gcaaaatcag aagataaaat tgtgatggaa ttgtctagct tccaactcat gggaatcaca
    29221 gatttccgcc ctcatatagc ggtgattacc aatatctatg aagcacatat tgattatcat
    29281 ggtacaagaa aagaatatgt caaagctaaa tggaaccttc aaaaaaacat gacagaaaaa
    29341 gattatctga tccttaactg gaatcagtca gaactacaag aattagcgca aagaacaaaa
    29401 gcacgcgtac tgcctttctc gacaaaagaa gtgttggaag acggtgtgta tgctgacgat
    29461 tactcaatat actataaaaa agaaaaaata atggaaatat ctgaacttgg cgtaccaggt
    29521 aagcataatg tagaaaatgc actagctgct atttctgtag caaaattgta tggtatatca
    29581 aatgaagcta tacgagaaac gttgcatttt ttccatggcg taccacatcg tacgcaatat
    29641 gtaggagaaa tccaaggcag aaaattttat aatgattcaa aagcaaccaa tattttagcg
    29701 actaaaatgg ctctaagtgg atttgaaacg tctaaggtcg tgttattggc tggcggatta
    29761 gacagaggaa atacatttga tgaattaatt ccttcattaa aaggaatcaa agcaatggtc
    29821 gtatttggcc aaacaaaaga aaagttgatg gatgctggaa aaaaagcagg catcgagaca
    29881 atagtaacag ccgattctgt agaacaagca gttcctttag cattagaaaa cagtacagat
    29941 ggtgatgtag ttttattatc accagcgaat gccagctggg atcaataccc aaattttgaa
    30001 actagaggaa acagattcat ggaagctgtg aaccgattaa aatagaaaaa gtagaagcga
    30061 gcgataaaaa atgaaaattt tggttacagg cggaggaaca gggggacata tttatccagc
    30121 tcttgccttt gtcaattatg taaagactca agaaccgaat gctgaattca tgtatgtggg
    30181 agcaaaacga ggtctagaaa acaaaatcgt ccctgataca ggaatgcctt tccatacctt
    30241 ggagattcaa ggatttaaac gaaaaatttc catgcataac atcaagacgg tccgattgtt
    30301 tcttaagagt atccgtcaag caaaaaaaat cctcagtgat tttcaaccag atatcgttat
    30361 cggtactgga ggatatgttt ccggagcggt agtgtacgct gcagccaaaa tgggtattcc
    30421 aacagttatc catgaacaaa acagtgtacc aggtgttacc aataaatttt taagccgata
    30481 tgtggatcgg atcgctcttt catttaaaga tgcggcttcc ttttttcctg aaaacaaagc
    30541 agttcttgta ggtaatcctc gagcacaaga agtagcagat actaaaaaat cagatatttt
    30601 gaaaacattt gatttggatc cagaaaagaa aacagtcttg atttttggcg gtagtcaagg
    30661 agcacttaaa atcaatcaag cagtatcagt ttttttaagt gaatatattc cttcttctta
    30721 ccaagttttg tatgcttcag gagatagata ttatgaagag atcctaaata aaataaatcc
    30781 tgaaatacaa aatattagtg tccagcctta tatcaaaaat atggcagagg tcatggcttc
    30841 cagtgatctg ctggtggggc gtgctggtgc aacttcgatc gcagaattca cagctctggg
    30901 gttaccggca gtgttggttc ccagcccata tgttacgaat gatcatcaga ctaaaaatgc
    30961 aatgagtctt gttcacgcag gagcagcaaa aatgattgca gataatgaac taacaggtga
    31021 atcactaagt cagacagtca acgaaatcat gggtgatgaa gaattgcaaa agcaaatgtg
    31081 ccgagcttct aaagaacaag gaatccctga tgcttcaaaa cgtctttatg acttagtaaa
    31141 acaaataatc aaaaaataaa ggcggtggtc ttagtgagta agaaaaaaga accaaaaaaa
    31201 ttgtctcatc cgtcttctga tgaaaacaat ttgactcctt ggcaaaaggc taaccgaaaa
    31261 tatctggcag aacaagagat gaaaaaccaa gagccaaaaa aagaagacat aacattagat
    31321 acaccccttt cagattctga agaagaaaaa acggttcaag aggaacagca agaagaaagc
    31381 cagtccgaga aaaaaatcga attttttgaa gagatcaagc aaaagaaaaa cggtggacca
    31441 tataacggtt cttttttgaa ccggctgccc aatttaaaaa gtcaacggca caaagtacta
    31501 tttcgtcggt tagctttgat catcactgta ttgggtatac cgctgatttt tttgatctat
    31561 tatgtttctc cttatagcaa attacaagca gttgcagtat caggcaataa gacagtcaac
    31621 agccaagaaa tcatctctga tacaaaattg tcattaggag aaaatgtttg gggacagtat
    31681 tttcatcgat ctacttatat cgatcgcctt aaaaaagcac agccaagaat cgaaacagca
    31741 aatatacatt tcaaagggat gaatgaattc gatttggatg taacagaata taaagagatt
    31801 gctttgatcg caaaaaacaa tcaatactac ccagtgattg aaaatggaac agtattagat
    31861 gaaaaagtag caaatcctac aaaaaatctc cctatcttgg aagagttcaa agactctgca
    31921 aaaatcaaag aattaacaaa gcaatacaat cagttatcat cagaattaca aaaagcaatt
    31981 tctgaaatca agtacacgcc aagagcaagc aataaaaatc tgatccaatt gaatatgaat
    32041 gacggcaatc aagttattgt aaatatcaat aatcttgcga accaaatgaa atattattcg
    32101 caagtagcca aagatatgga tgaaaaaggt gtgatcgaca tggaagttgg tatcttctcg
    32161 catccttacg gtagcactca agaaacggaa gaatcgggcc aatcggaaga aacaactgaa
    32221 agtgaagaaa atgcggatca aacaaacgaa tcaagtgaag aggcagaaac gcaaaaagca
    32281 caaacacctg gatcgtctag cacaaacgga tcatctgatt caaatgattc aagtgaaaca
    32341 agtcaaatag aagataatcc aagtacaact ccgtcaagtt taaatgaaaa ttgataaaac
    32401 aactttaaaa agtaagaatg tactttctaa aaaccagtaa tttatggtag aattggaagc
    32461 agtgaatata tctattaata agtatatttt aaaacggacg ctagttgttc gcttgtatat
    32521 ggaatagtaa gtaggaggga atcccattca tggcaaaaac aggaatgtat gtaggccttg
    32581 atattggaac gacatctgtt aaagttgtcg tggctgaata tatcgacagc caaatgaata
    32641 ttattggagt aggaaacgca aaatcagaag ggattaaccg aggcattatc gttgatatcg
    32701 ataaaacggt ccaagcgata caacgagcag tacgacaagc agaagaaaaa gcaggtatcc
    32761 agatcaaagg cgtaagtgtc ggtttgccag caaacatgct tgaagtagaa aattgccagg
    32821 gaatgattgc agttaacgga gattcaaagg aaatcaccga tgaagatgtc agaaatgtgg
    32881 cttcagctgc ccttgttcgt tcaatacctc ctgaacgtca aatcgtgtca atattgccac
    32941 aagattttac tgtcgatggg ttcgagggaa tcaaagatcc tcgtggaatg atcggcgtaa
    33001 gattagaaat gtacggtctg ttgtttacag gacctaaaac gatcatccat aacatacgta
    33061 aatgcgtgga aaatgccgga ttaatcgtca acgaaatggt gattactcct ttagcgctga
    33121 ctgaatcaat tctttctgac ggagaaaaag attttggtac gatcgtcatt gatatgggtg
    33181 gcggccaaac aacaacggct gttatgcatg ataagcaatt gaagtttacc aatcttgacc
    33241 aagaaggtgg cgaatttgtc actaaagata tctctattgt attaaatacg tcatttaaca
    33301 atgcagaagc gttgaaaata aactacgggg atgcataccc agaacggaca tcaccagatg
    33361 aagaatttcc agtagatgtg attggtcagt cagaaccagt gaaagtcgat gaacgctact
    33421 tgtcagaaat tatttcagct cgaatggaac agattttcaa caaagcaaaa gatgcattgg
    33481 accaaatcga agcattagag ttgccaggtg gcgtcgtatt gacaggcgga gctgctagtc
    33541 ttcctggtgt tgtggacttg gcacaagaaa tcttcggcgt aaatgtaaaa ttgtatgttc
    33601 caaatcacat ggggctgaga aacccggtat ttacaaatgt aataagtatc gtggattatt
    33661 cagcaaactt gagtgaagtg tatcaattgg caaaaattgc tgttacgggt gaaacctcag
    33721 cagctcgtca aatggtagtt gaacaagaaa caacaaacac atatgagagc tatgaagcac
    33781 ctgaagaaac aatctacgat gagccggaac caaaagagtc cggagaaaat gtaaaaaata
    33841 aaatcaaggg tttctttaca aatatttttg attaattcat cctgggagga ataagatatg
    33901 gaattctcaa ttgataataa tatcaatgac ggcgcagtaa tcaaagtcat cggagtcggt
    33961 ggtggcggtg gaaatgctgt aaaccgaatg attgaagaaa acgttaaagg tgttgaattc
    34021 attacagcca acacagacgt gcaagcatta aaaaattcaa aagctgaaac agttatccaa
    34081 ttaggcccca aatatactcg tggtttaggt gctggttctc agccagaagt tggacaaaaa
    34141 gcggcagaag aaagtgaaca atccttacgc gaagctttag acggtgcaga tatgattttc
    34201 atcactgcag gaatgggtgg aggaaccggt acaggtgctg caccaatcgt tgcaggtatc
    34261 gcgaaagaat tgggcgcatt gactgttggt gtcgtgacac gtccattcac ttttgaagga
    34321 ccgaaacgtg gccgttttgc tgcagaaggt atcgctcgtt tgaaagaaaa tgtggataca
    34381 ttattaatta tctcaaataa ccgtttatta gaagttgtag ataaaaagac accgatgctt
    34441 gaagctttcc gtgaagcaga taatgtttta cgacaaggtg tacaaggtat ctctgatttg
    34501 atcacagcgc caggttacgt taaccttgac tttgctgacg tgaaaacagt gatggaaaac
    34561 caaggtacag cattgatggg gatcggtgtg gctagtggag aagatcgcgt agtagaagca
    34621 acgaaaaaag caatctcctc tccattgctt gaaacatcta ttgatggagc tgaacaagta
    34681 cttcttaaca tcacaggcgg tttggatatg acattgttcg aagcacaaga tgcttcagat
    34741 atcgtagcaa atgcagcaac tggcgatgta aatatcattc taggtacatc tatcaatgaa
    34801 gaaatgggcg atgaaatccg tgtgacggtc atcgcaacag gtatcgatga atcaaaaaag
    34861 gaacgcaagt ctagccgacc agctagacaa gcacaaatgc aatcacctgc tcaaaagact
    34921 gtattagata tggatcaagc taaaccaact tcttcagaag aagaaaacag ttttggcgat
    34981 tgggatatca gacgtgaaca aaatgttcgt cctagagttg atgattccaa ttttgacaat
    35041 atcgaaaaaa aagaatttga tacattcaac cgcgaagaaa caaaatctca tggcgatgat
    35101 gaattaaata cgccaccgtt tttccgtcgt aaaaaataaa ggagaggaag agcacatgat
    35161 tgctgacaac ttgcagaaaa tcaagcaaga aattcaggat tcgtgtgctc ttgttcatcg
    35221 tgatccgaaa gaagttacat tagtagctgt aacaaaatct gttgaatccg ctcaagctga
    35281 agaattggtt agagctggcg ttatgaattg tgcagaaaac cgagtagata aattattaga
    35341 aaagaaacaa gatttatttg attttcctga aataaagtgg catttaattg gtaatttaca
    35401 gcgcagaaag gtaaaattga ttgtaaatga aattgattac tttcatgcgt tggacagttt
    35461 aagattagca gaagaaatcc agaagcggtt agagaaagaa ttatcctgtt ttattgaagt
    35521 aaatgtttct ggtgaggaat caaaacatgg aatttcacca gatgagcttt taccgtttgt
    35581 ggaatcttta gctgcctgca caaaaatcaa ggttgttggg ttgatgacta tggcgcctaa
    35641 agaagcttct gatgagcaaa tccataaagt gtttggtacg ctgaaagaat tgcgtgatct
    35701 tgttcaagca aaaggttatg cccatgcacc atgtagtgaa ttaagtatgg ggatgagtca
    35761 agactttcaa attgcgattg aagaaggtgc aacatttatc cgtgtaggaa cagcgctatt
    35821 caaagacgaa taaaggagga acaaaaatgc cgcttttaaa cagaggagca attgctaatt
    35881 ttttcggatt agctgatgag gaagagtacg aataccaaga ttatcctgaa acaacaaaaa
    35941 aggctgtaca gccaactaaa aaaaatacaa cacagaatca atcaactcat tctgtatctg
    36001 ctgcgaatac tcatgaaaaa caaaaaacgg cgacttcacg tcctgtaacg catgaaagta
    36061 ttcagccggc tgaaaagaaa aaagtaattg aactacatca atcacctaca aaaactgtag
    36121 cgaaagaaag aacagcttca agaacctcta cacaacctaa agcaaatctt gctggaaaaa
    36181 tcactgtaat ggaaccaaga gcttattctg aagctatggc gattgctaaa cgaatcattg
    36241 ctggagaagc aaccttagtg aattttcatt tagtagaaga aaaacaagcg agaagaatcg
    36301 ttgattttct aacaggaaca gtttatgcac tagatggaga tatcaaacgt gttggagatg
    36361 agatttttct ttgtacaccg actgggatgg agattgacag ttcaaccgct caatcttttg
    36421 cagatgcgaa tttgtttgat ctgtaagagg aggaactacg atttatacta ttgcactatt
    36481 acttcattta cttcaacggg cagcctattt ctatagcatt atcttgatgg tatatgcatt
    36541 gctctcttgg tttccaggcg ggtatcaatc agggctagga cgattattag ctaagatctg
    36601 cgaaccgtat atcagtttgt tcgatcgtct gcctttgagg attggaccct tggatttcac
    36661 aattttaggg gcaatcctga tcttgaactt agccgtacaa ggtttagcac agatcgttat
    36721 atggataact gtccatttag tttattgatt gaagcaacaa aatctttatt ctcagaatag
    36781 ataggagggg aaaattttgg atgcaaatgt ataccagcac tttcgggcag atgaacgtac
    36841 atttatcgat tcggtaggcg attggattga acaggttcaa ggacaatatg caccctatct
    36901 gaccgaattt cttgacccaa gacaatccta tattttagaa acactgattc gacaaagcag
    36961 tgatttgcgt ttcatgtttt atggcggata tgaacaggca gaaagaaaaa gatgtttgat
    37021 tttccccgat tattacgaac ctgtagagga agattttgaa acagcattgt atgaaatcca
    37081 ttatcctagc aaatttgcca gcctaagtca tgggaaaatc cttggaactt tagtgggaac
    37141 ggggatcaaa cgctcttact ttggcgatat catctcggac ggtgatcgct ggcaagtatt
    37201 tattgcaaat gaggtggatc attttgttgc tgcacaagta acaaagattg gaaaggttgc
    37261 tgtacgcttg gaaccaatca aatacacaga aatgattatg ccgaaagata gctggacaca
    37321 agaaagagga acagttagtt ccatgcgact agacagtgtg atctctgaga tttataatat
    37381 ttccagacag cggtccaagc agctgatcga agctggtaaa gtaaaagtga actggacaga
    37441 aaatacaaga cctgattttg tacttgaact attagatatt atttcagtca gaggttatgg
    37501 cagagtccag atccaagaac ttgaggggaa gacgaaaaaa gaaaagtaca gggtactgtt
    37561 cggtgtatta cgtaaataaa ataaaaaggt ggtttactca tggctttaac tccattagat
    37621 atccaaaata agacattccc gacaaaaatg cggggatata atcaagacga agtagatgat
    37681 ttcttagatt tagttgttcg tgactatgag gaattgactc aaagaaatcg agaattagaa
    37741 aaagcagtca aacattctga agaaaaactt gagtacttca atgaattaaa agatgctttg
    37801 aaccaatcaa tcatcgtagc tcaagatacg gctgataaag taaaaacaag tgcaagcaag
    37861 gaatccgaag tgatcgttac ttctgcacaa aacaaagcag atgagttagt tgcgaacgct
    37921 gaaaaacgtg cacatcaatt aacaactgat gcagaagaaa aagcaagaaa aatcttgaca
    37981 gatgcaacag aaaaagcaag acagcttgcg acagaaactg aagatcttaa gaaaaaaaca
    38041 cgagtattcc atcaacgtat cagcttgatg cttgagtctc aattagaaca agtgaaaagt
    38101 ccggaatggg atgaaatttt acaaccattc tccagctacg tgactgacag ccatgaagtg
    38161 atcaaagaag ttttagcaaa acagcttgac aatgaaaatg atactgatgt aaactcagac
    38221 acaggagtca tagaagcacc tatgacggca tttgatacac aggctattga tatctcgatc
    38281 gtgcctggtg aaaatgaaaa cgagtagaac agaaaagcac tttgttattc ttaaagcgag
    38341 ccgatgagag tggaagttcg gtaagacact gcatggtgaa agatcctcta cttgagacct
    38401 gcttttccga aattagagta agaagcagcg gctgacctcg ttattggtca cagagagttt
    38461 gcactagata gatgcaacta aatttgggtg gtaccacggc atgttcgtcc cttgcggctg
    38521 aacatgcttt tttgtgtccg gaaaacaaat aaaggagtgt atggttgaat gaaaatgaaa
    38581 gaaaccttgc aattaggaaa aacaaaattc cctatgcgtg gtaatttgcc aaaccgagaa
    38641 ggcgagtggc aaaaagaatg ggaagaaaat cgaatctacc aaaaaagaca agagttgaac
    38701 gaaggaaaac ctagctttgt tcttcatgat ggacctccat atgccaatgg taatatccat
    38761 ttaggacatt cattgaataa aatcagtaaa gacattatca ttcgttcaaa atcaatgtct
    38821 ggctatcgtg caccatatgt tccaggatgg gatacacatg gattgccgat tgaacaagta
    38881 ttggctaata aaggcgtgaa acgtaaagaa atgtccatgg cagaatactt ggaaaaatgt
    38941 cgtgaatatg cactttccca agtagataag caacgagcag attttaaacg cctaggagtt
    39001 gcaggagatt gggaacatcc ttatgttaca ttagatcctt cttatgaagc tgcacaagta
    39061 cgcgtatttg gtaaaatggc tgaaaaaagc tatatttaca aaggattgaa accaatttac
    39121 tggtctccgt caagtgagtc ttctttagcg gaagctgaaa ttgaatataa agatgtaaaa
    39181 tctccatcta tttttgtagc tttcaatgtc aaagacggca aagatattct tgatgaagat
    39241 acagcttttg tcatttggac gacaacacca tggacattgc cagcaaacca aggtattgct
    39301 gtaaatccaa aatttacgta tgtattggtg gaagccgatg gccgtaagtt tgtcgtagcg
    39361 aaagatttgc tagaaacagt ccaatcagaa atcggttggg aaaatgtaac agttctaaaa
    39421 gaatttgctg gacaagaaat ggaatatatg actgcaagtc atccattcta tgaacgtgaa
    39481 tcacttgtga ttttaggcga tcatgttact ttagacgctg gtactggatt agtccatact
    39541 gctccaggac atggggaaga tgactatatc gctggtagca aatataaact atctgtagtg
    39601 tcacctgtag actcaaaagg attgtttaca gaagaagcac ctggatttga aggtatcttt
    39661 tatgacaaag caaatcctat gatcactgat ctattaaaag aaaaaggagc tttgctgaag
    39721 ctagatttct ttactcatag ttatcctcac gattggcgga cgaaaaagcc tgtgatctat
    39781 cgggcaactc cacaatggtt tgcatctatc gataaattcc gtcagaatat tttggatgaa
    39841 gtagaaaaag tagagtgggt tatcccatgg ggtaaaacac gtttgtataa tatgatccgt
    39901 gaccgcggcg attgggtaat ttcccgtcag cgtgcatggg gagtaccact tccgattttc
    39961 tatgctgaaa atggagaagc aatcatcaca ccagaaacaa tcgaacatgt ggcaaaatta
    40021 tttgctgaac atggatcaat tatctggttt gaaaaagaag cgaaagaact tcttcctgaa
    40081 ggatttaccc acccaggttc tcctaatggc gaatttacaa aagaaaaaga tatcatggat
    40141 gtctggtttg attctggttc ttctcatgaa gctgttttac gtcagcgacc agaattatca
    40201 ttcccagcag atatgtattt agaaggatca gaccaatatc gtggctggtt caactcaagt
    40261 atcacgacta gtgtggctat caatggtgta gcaccataca aatcagttat ttctcaagga
    40321 tttgctttag atggtgaagg ccgtaagatg agtaaatctt taggaaacat cattgcacca
    40381 gaaaaagtga tcaaacaatt tggtgcagat atccttcgct tatgggtatc tagtgtagat
    40441 tatgaagcag atgttcgtgt ttctatggat atcttaagtc aagtggcaga agtatatcgt
    40501 aaaatcagaa atacgatgcg tttcttatta gcgaatacag aagatttcga tcctaaaaaa
    40561 gatactgttt cgtacaatga tttacgttct gtagacaaat atatgaccgt tcgattgaac
    40621 caagtaatca aagaaatccg agaagaaggg tacgacaaat ataacttcat gcatatttac
    40681 cgtacagtta tgaacttctt gacggttgat ctatcgtcat tctacctgga ttttgctaaa
    40741 gatgtagttt atattgaagc agaagatgat tatcaacgtc gttgcatgca aactgttttc
    40801 tatcaaacag ctgtagcttt gacaaaatta ttgactccta tcattccgca tacagcagaa
    40861 gaaatctggt cattcctgaa agaagaagag gagtacgttc agttaagcga gtttcctggt
    40921 tatgaagagt ttgcaaacca agaagaattg atggatacct ggactgcttt catggacttc
    40981 cgtgataatg tcttaaaagc attggaagaa gcaagaaaca gcaaattgat tggtaaatct
    41041 ttggaagcaa aactaactgt ttatccaaaa caacaagttc gtgaaatgtt aaaagctttg
    41101 gatgcagata ttgcacaatt attgatcgtt tcaccagact actttgaagt tatggcagca
    41161 gatgaacaag tgccagataa cgctatggta tttgatgatg ttgctattac agtggaaaaa
    41221 gcagacgggg aaacttgcga tcgctgtcgc caagttcgta aagatgtagg agtagatgaa
    41281 aaacttcctc atctatgcgg tcgttgtgca aaaatcgttg aagacttcta tcctgaagct
    41341 gtagctgaag gattcgaaga aaagtaaaag ggaaaagggg gtgtgacatg ttctttgtca
    41401 cacccccttt tgttatcaat cgtttaattt tccacgttca cgataccagt tatctggttg
    41461 ccagatccat tcgaatccgt ctttttcaag tagttcgaaa gctgctttag gtcccattga
    41521 tccagctttg tagttgggaa aatccggttg tgtttggtcc caagcttttc gaatgacatc
    41581 cacgatatgc caagattgag cgacttcttc ccaatgagag aagttcgttc catcaccatt
    41641 caacgcatct aatagtaatt tttcgtaagc ttctgggcta ttttcaacca tttctgcact
    41701 atttctaaat tccaatttga ttggttccgt ttcaaatcct tgccctactt ctttcccatt
    41761 caaggataaa gaaaatcctt cagttggctg aatatagatc gttagaacat taggtggaag
    41821 tgtcgtatcg tcacatggtt catcgacaga tgttttgaat acattaacag gaacttgttt
    41881 gaatacgatg ttgatccgag tccctttttc agtcaaacgt ttgcctgtac gtacatagaa
    41941 aggaacacct gaccaacgga agttatcgat agtgaatttg cctgcgacga atgtttcagt
    42001 ctctgattga gcatccactt tgtcttcttc gcgatagccc ttgaattgta caccatccaa
    42061 ttcaccttga gcatattgcc cgcgaacgaa gttttctaac acttcttctt cgttataaat
    42121 gcggatagct ttcaatgctt tgatcttttc tgtacggatt tctttttcgg aaaatgctac
    42181 tggcggttcc attgcaagta atgctacaac ctgtaaaata tgattttgca ccatgtcttt
    42241 aagtgcgcca ctatgatcgt agtagccccc gcgatcttct acaccgaggt tttcgccaaa
    42301 agtaatttgg atattgtcga tatagcgatt gttccattga gcctcaaaaa tattattggc
    42361 aaaacggata gctgagatgt tttggatcat ttcttttcct aaataatgat caatcctgaa
    42421 aatatcttgt tctgggaata cttctctgat ttgctggttc aattcaaaag cagactggta
    42481 atcagaaccg aacggttttt ctatgatcaa acgattgaag ccattttttg tcataatatt
    42541 ttgtgatttc aaatgagaaa cgatcgttcc aaaaaattga ggtgccatag ccaaataata
    42601 gacacggtta ccgtttaatt gatagtgtcc atctaatcgg tcagacaatt cttttaatgt
    42661 ctgataatgc gttgaatcat ttacatcatg ggattgatag taaaaatggc ttgcaaaggt
    42721 agttgcttct tcatctgaag gattcaatgc acggattgtt tctttgacaa cttcacggta
    42781 gtgctcatcg ctccagggac ggcgagcggt tccgattaca gcaaaatgct cgcctaaatt
    42841 tccttttctg taaaggcgga acaaagacgg gtacaattta cgctgggcta ggtcacctgt
    42901 accgccgaat atcgtaaaca atacattttt ttcattagac atgtggatac actcctttat
    42961 attttatcga caaaaatcgt gctggccgcc ttaaagctga cggggatttt ctttctatca
    43021 ttttggatag tgatcggtcc ttcataagct gcgatgtcaa cgatctcata ttcttcgttg
    43081 atattcaggt caatagtgac taagtaatcc aacaattctt tttcatcaag cactcgtgcg
    43141 atacgcactt ttgtaccgac aggataggac tccaatgttt gtcggcgttc ttcatggaca
    43201 acttcctctt ttttaggaat cgatcctcca tgaggacaat gttcaggatg attcaaatag
    43261 ttttctaaat gatctgcaag atgttccgaa gtcacatgtt ccaatacttc agcatcatca
    43321 tggacttcat tccagctata gtctaaatgt tcaactaaaa aaacttccca taagcgatgt
    43381 ttgcgaatca gactgctagc tttttgcagt ccgcttgcag tcaattgtac accttgataa
    43441 ggggaatgtt ctacaagctc ttctttcaca agtttagaaa tcatttcact gacagatgca
    43501 gctgagacat ctagactaga tacgatttgt ttattgttga ctttcgttgt atctccacct
    43561 agttctaaaa tgattttcaa ataatcttct cgatttggtg tcatattcga tcatcctttc
    43621 atggtaaaaa accatcatta gtaagtgtaa caaaaaaaac gttaattgac tatttttcta
    43681 aataaaaaac aaaggatttt tagacgtttt tacaacaaaa aacgaaagat gatagagaag
    43741 actcattctt tcgtttataa aggatatata tatacattgg gagggatcta tttttcagat
    43801 aacaacttat atgaagtgtc tgcagattcg tcataaagag tttgttcggg ataagaaaag
    43861 ataagataaa aaccatactg atcacgtacg actaaacgat attcatctat ttctaccaca
    43921 gatgtgccaa gagactgacc gttgatcaaa aacgtgtaat cagatgtaat ctgtaattgt
    43981 tgtttttgct ggctatcact aaagaaccat gtaccttgat actttgagag tccttctgac
    44041 ttttccgctt gttttttctt tttcaagtaa ataaaacttg agagaacggt aatgagtgaa
    44101 ccaagaaaat agatcatttt ttttatcacc aaatcactcc tttatgagaa aataatcgca
    44161 tagacatatt ctttcttctt tatttcattg taccgaataa aagctaaaat ttctctaaga
    44221 ctttcgactg gtttttttac tgtttttcta caaattatga taggatacat agtaggaaga
    44281 aaggtgacat aatttgaagt atcagcaagt ggaaatagct ttttttattg accgcccgaa
    44341 tcggtttata gcacattgta tgaaaaaaga cggagaaata attattgcgc atgtcaaaaa
    44401 tacaggtaga ggaaaagagg tcctgatacc aggcgctgaa gtggctattg tatttgctcc
    44461 tggacctaaa cggaaaaccg cctatgatct gattgctgta aaaaagaagg gtgactggtt
    44521 taatatcgat agtcaattgc ctaatcggct ggcaattgat ggtattttag acggcacgat
    44581 ccttttacca ggattaactg gtccatttac ggtatacaag cgtgaagtaa cttatcttca
    44641 ctccaaattt gatatttatt tagagacggt ggtcggacaa aaagcatttg ttgaagtcaa
    44701 aggtatgaca cttgaaaata aagcgattgg tgcttttcct gatgcgccca cactcagagg
    44761 attgaaacat ataggagaat taacgtatgc cgcacaagat ggttatgccg cttatgtctt
    44821 gtttattgct cagttcgaac atcttcatct ggcaacgatc catgaagaaa tgcaaccagc
    44881 actagctgat atggttcgac atgcgcagca aagcggagtt caaatcctcg cctataattg
    44941 tcaagtatcg gcagatgagg taactgtcaa gcaggccatt ccatttgatg tagatatccc
    45001 ttttgaagat cctatacaat agaaagaagg taaaataatg atcaacttag gtattatcgg
    45061 aacaaattgg attactcatc aatttgtcca agcagcttta agcacaaaaa aatatgaatt
    45121 gacagcggta tattctagac atttggaaaa agcacaggaa tttggttctc attatgaagg
    45181 ggatatcgaa ttcgcaacag atttgaagac ttttttcaat attactcata tggacaccat
    45241 ttatattgct tctccgaata gtcttcactt tagtcaagct aagcaagcaa ttttggcagg
    45301 aaaaaatgtg atagttgaaa agccagcttt ttcgacgcca aaagaaatgg atgaaatcat
    45361 ccatttggca aatgaacaca atgtgtattt ttttgaagca gcaagaaata tccatgaatc
    45421 aggttttaaa aagattgccg attacttacc attaaaagac gagataatcg gtgcaaattt
    45481 cagctatatg aaatattctt cgcgctatga ccaagtatta gatggagaag aaccgaatat
    45541 cttctcgact catttctcag gtggtgcatt gatggattta ggtgtttacc ttgtatatgc
    45601 agctgttgct tggtttggaa tgccaaaaga agtccattat ttcccaagaa agatttctac
    45661 tggtgtagat ggtcttggat gggggatctt acgttatgag aattttgatg tatcgatcca
    45721 gccaggtaaa atcggtgatt cttatttacc gtcagagatt tattttgact caggaacgct
    45781 agtgatgaat ggagtcaatg caattgaaca agcacaattt catgatcgcg cgcataaaga
    45841 aatcattgat ttggcggtaa cagctgcaga aaatccaatg gcagatgaag cagaggattt
    45901 tgccaatgtc atggctcatc cgaaagatcc agcttgggga ttgttgtatg aagagtggat
    45961 cgagctagct agaaatgtga accaagtgat ttatgattta agaaaaaatg gcggcatcaa
    46021 attcgatgca gataatgagg aagattttta atgaacgaat ttgtacaagt atttcccgaa
    46081 aattggcaga aaaaatatga ggataaaggg tttatctcac cttctcttat tcaacgagca
    46141 gtttttcagc cactaagtaa taaacaaagt atagtagcgg tttctcctac aggttcaggt
    46201 aaaacactgg cttatctttg gcctctacta ctaaacgtgg aaccaggtga agcaagtgcg
    46261 ttggtcattt ttgcttcttc tcaagaacta gctattcaag tagcagatgt tgcacgtgaa
    46321 tggggaaaag acaaagagtt gaaagtccaa tctttggtgg gcggagccaa cgtcaaacgt
    46381 cagattgagg gtttgaagga aaaaccagaa atcttgattg gtacaccagg aagaatattg
    46441 gaattgatga aagcaaaaaa aataaaagca catcaagtga aaacaatggt atttgatgaa
    46501 gcagaccagc tatttgacgc tgggaacagc caaatcattg atcaaattct tcatcaagct
    46561 ccaacagagt atcagctagc ttttttttca gcgacagcag atcgttccct tgaaagcatt
    46621 gaaaaaatca ctgggaaaac gtttgagaca attgacgtga cagcagagga cgattctcga
    46681 aaaggactga gacattactt tttacgtgta ccgatacgaa agaaagatga ctacttgaga
    46741 cgtctcacgc atatagaaga ttttcatggt ctgattttct ttaatcaact gactgaatta
    46801 ggcgtaatgg aagaaaaatt gctgtataga ggtgtaccgg taggaagtct ggcctctgat
    46861 cagaacaagc tactccgaaa agcagcgcta gaacaattca aaaagaaaat ggtcagagca
    46921 cttttgacca cagatgtggc ggctagaggt ttagatatta caggtcttcc ttatgtagtg
    46981 aatgcagaag ttccgttaag tgaagaagct tatttgcatc gttcgggaag aactggtcga
    47041 atgggtaatg aaggaatagt aattacactc gtacaagata ataatctaag agatttaaaa
    47101 cggcttttac gtccatcaaa tataacactt gaagagattt ttttatacgg aggtaagctg
    47161 caaactgagc agccaaccaa agaagatgca gctcctgcag taaataaaaa gtatgcgaaa
    47221 actttttctg gaaaggagca gaacccagaa tataacaaag aaaaaataag aaaaaaagtt
    47281 aaaaataaat caaaaaaaga gagaaataaa ggagcaaggc gcaaataagc gcctttttat
    47341 gctatgatta tgaaaacggg aacaaaattg gttgccagag gaggggttat gtgaaagaac
    47401 gtcaagaaga gatcttgcag ctattgacag aaaaccaaca aggattgaca gcaagtgatg
    47461 tagctgagcg cttaactatc gatcgaagca atgcaagccg atatttaagt gaactataca
    47521 aggcccatca tattgtaaaa accgctggcc gaccagtcgt ttactctttg ccaacggaaa
    47581 agtcaaaatc tgatgaagtg catgtcgact cttctacgca agtgactttt gaaacactcg
    47641 ttggagaaaa tgattcttta aaagtcagca tacagcaggc aaaagctgcg attctttatc
    47701 caccgcgggg gttgcatacg attatttttg gagaaactgg tacagggaag tcgatgtttg
    47761 ctgaatgtat gtatcatttt gcaatcgatt cagagatgct ttctgcagat gcgccatttg
    47821 tttctttcaa ttgtgcggat tatgctcaaa atccgcaact gctctttgga cacatctttg
    47881 ggatcaaaaa aggggcttac acaggagcag ctcaagatag tcccggtcta atagccaaag
    47941 cagacggcgg tatattgttt ttagatgaga tccaccgttt accgccggaa gggcaggaaa
    48001 tgctgtttac tttcattgat aaaggtattt accgtccgct tggagaaagt gcacaagtac
    48061 atgaagcatc tgttcagatc attggggcga cgactgaatc ttcagaaagt ttcttatcta
    48121 cgttcaatcg acggattcct atggcaatca ccttgccgaa tcttgcttca cgttcactgg
    48181 atgagcgtta tgaaatcatc tctcttttta tcaaacaaga agcgaatcgt ttgaatcaac
    48241 gtatcgatat cgaaaaagaa gcgattcttg ccttcatgct ttatgatgcg gaaggaaata
    48301 tcggtcaagt gaaacgagac ttgaaactag tctgtgcaaa atctttttta cattaccgta
    48361 ctcatcatga gaaaaagcta atcattcgaa aagaagaact ttcccttcaa gtacaaaaag
    48421 gattactgaa aatcaaggaa ataccggaac gtctcgaccg attcatggac agcaaaagtc
    48481 agtatttgac atttgaacca ggatttgctg atgttgtctg gtcacaagat ccagaacgaa
    48541 acatgcaggt ctataacgat attgaggaaa aagtcacttc tctttccgaa accggtgtgg
    48601 agaacattga tctagagacc ctcatttcaa aagatgtgga tgcttatttc cagacatatg
    48661 tcaaagaatt gactaaaagt tcgattccca aagatctttt gccagatgaa atctggcggt
    48721 taaccaatca attgtatgat gtggctgaaa aacggttaga ccgtatttat aatgaaaaag
    48781 ctcgttttgc ttttgctttg catctgcaaa gtacattgga tcgtgtgaga gaaggtcata
    48841 tgatcgtcca cccagatttg aataacgtac ggaaaaattt taagagggaa ttccaggtag
    48901 caatcgatct ttcaacgatc attgaagaag aagaacaagt tgagattcct tttgatgaaa
    48961 tcggctttat tagtatgttt ctttctatca atctaggaga aagtgagcct gttaaagaaa
    49021 acaaagtaga tgttctcata ttgatgcacg gcagacagac tgcttcaagc atgttggaga
    49081 tggcacaaga actattagga acaaaagtag ggaaagcttt taatatgcca ctggttatgg
    49141 aagttcaaac aatgtatgaa gaagtgttac aatatgtcaa aaactatcaa gagcatttga
    49201 cgaatggcct actcttgtta acagatatgg gatcattaaa caccttcagt gatttgattt
    49261 atgaagagac tggcattcgg acgaaagcta tcacgatgac aagtaccatg attgtgatag
    49321 aagcgatacg gatggcaagt gttggaagaa gcttagaaga tatttatcaa aatatccagc
    49381 tttcctttga atcgatcgta cgtgatcagt tcaaagcgga aaagtctgaa actttacagc
    49441 gtaaaaaagc cgtaatcgtg acatgcttta ctggtgaagg agtagctgcc aaattatatc
    49501 agcgtattat gccagtgatt aatcaaaata aggtagaact gatccagatg cagtttttag
    49561 aacgtgaatc attcaaaaaa cacatcgatg ccttattgga agaatatgag atcaaagcaa
    49621 ttgcgggaac agttgatgtt gattatcaaa atatcccatt tttttcagcc tatgacgttt
    49681 ttgatgatga aaaactaaat attttaaagc gtatagctga tgatgagatc ccgacagcta
    49741 aaattgtcga ttcacttgct ggatcattaa gcgatgtctt ttctattgat gaactgatcc
    49801 gttcattgca aaagacagtt cagcagatcc agaccgattt gtcgattatc gtggaacctg
    49861 gagtagatac tggaatcgtt atccatttag ccttcatgtt agacggattg atcaaacaag
    49921 aaagccatcg gcaatttgag aaactgtcta ttttctcaaa aatttatcgt ttggaaatgg
    49981 atatcgttcg ggcaaacttg atggcactag aacgaaaata tcaggtaaaa attccagaag
    50041 atgatatcgc atttcttaca caaatgtttc ttgaaaataa aataaaaaaa tgaacacact
    50101 aatcatactg tttacacact aaacatctgt gtgcgcacta taaaaaagag ctaaaaagca
    50161 tgaaaacgct atttcagctc ttttttttat tggcatgctt cttgcgttat ataaaagtgt
    50221 aaaaaaataa aaggagtgtt aaaaatggca aaaaaaacaa tcatgttagt ttgttcagca
    50281 gggatgagta cgagtttatt ggtcacaaaa atgcaaaaag cagcagaaga aaaagggatg
    50341 gaagccgata tttttgcggt ctctgcctca gatgcagata ataatttaga atctaagaat
    50401 gtcgatgtat tattattagg tcctcaagta cgtttcatga aagctcaatt tgaacaaaaa
    50461 cttgcgccta aaggtattcc attagatgtc atcaatatgc aagactatgg catgatgaac
    50521 ggagaaaaag ttcttgcaca agcagaaaat ctaatgaaat aaatcaagta tgcagttggg
    50581 ggtataacaa cgtggaagat caacaaaatc tagaagccat catggggtta atcatgtttg
    50641 gcggtaatgc aaagagcgat gcaatggaag cgattgcggc tgctaaaatc ggtgattttg
    50701 agttagcgga taggaaaatc gctgatgcag aagaatcact tatacaagcg catcatgcac
    50761 aaacagacat gttgacacaa gaagcacaag gaaatcatat gcaagtgaca ttattgactg
    50821 ttcacagtca agatcactta atgacatcta ttgcgtttac tgatttagca aaagaaatca
    50881 tcgaacttta tcgtcgcgta tacagcgaat aaatacaact tcttttttga acggaagaag
    50941 aaaaattttt gaacggattt tttattttgt tataaggaga actctcttta tataaaaaac
    51001 ttataggagg aagtaaaaaa tgaacggact atcaagttgg atggagaaat acatcctgcc
    51061 ggttgccgca aaaatcggag cgcaaaaaca tttagttgct ttgcgtgatg cttttatcgg
    51121 cacactgccc gcaacaatgg cgggatcagt cgctgtaatg atcaatgcaa ttattcgtga
    51181 tttaccgcaa cagtttaaca gtacctatgc tgacagttta gcagcagaca aaggaatctt
    51241 tgccgtattg aatgtcatca tcggaatcaa tggttttgtc tggaacggta cactagcaat
    51301 cgccggattg atctttgcct tttcttgggg gtataatctt gcccgagctt atggagtaaa
    51361 tgatctagct ggggggatcg ttggtttagc taccttgatt caaggtattt cctttggtcc
    51421 agcattatca gcagcattag atgtgaatgt tccttcaaat gtcgcagatg caatcaatag
    51481 ttctgagatt ggtgcaacga ttgcagatgg cacactgtca gttggattat ggggttggct
    51541 aaaactagat catttgaatg gaaacgctta ttttacagtt atgatcatgg gagctatttc
    51601 agtcattatt ttttgtaaat tgatgcttgc caacattacg atcaaaatgc ctgattcagt
    51661 accaccagca gtatctaaag catttgcagc cattttacca gcgacgatcg ctttatacgt
    51721 cattgctatt atcaattata tcgtaggtcg tgtagctgat gggcaactga tcattgattt
    51781 ggtgcaaaaa tatattgcag aaccattcct aggattatct caagggttgg gagcagtatt
    51841 gatcgtcaca gtatttgttc aagtattctg gttctttggt attcatggac caaatgtatt
    51901 ggcaccagtg ctagagggaa tctggggaca agcacaacta atcaatattg atattttcca
    51961 aaaaggttat aacggaaaaa ctggtacacc agccgtgtta gatgcaatcg aagatggaaa
    52021 agcttatatg tgggtccgcg gatcgttcga tgcttttgca tggtttggcg gatcaggcgg
    52081 aacgatcgtc ttactgatag ctatcttgat tttctctaaa cgtgcagatt atctaacagt
    52141 agctaaatta tccattgggc ctggtatctt caatatcaac gaaccagtga tgtttggtct
    52201 gccaatcgta ttgaatgcaa tcatgtttat cccattcttg attgctccag tagtagcaac
    52261 agcaattgga tggacagcta cttatcttgg cctagtcgct ccggtatcgc agcaagtcac
    52321 atgggtagta ccgccgttct tattgtcatt cttagcaaca ggagcggatt ggcgggcacc
    52381 gatcgtaaca ttagtatgta tggtcgtgac tttcttgatt tgggcaccat ttgtcatctc
    52441 tgcaaacaaa atggaccctg ccgcaggcga acaacaataa gatgaacgtg aggagcggga
    52501 cgctttaagt ttcgttcctc ataatgaatt ttcgaggagg aactgaaatg attacaattg
    52561 ccagtgcaat gcaacaagat gaaatcaaag aattgttaga aggatacaat gaagaaggaa
    52621 tgacttttac atttaaagaa aaacaaggaa tcaagctgtt ctttgaaaca agtgcagaag
    52681 atccggaagt tgcggctaaa aaagcaaagg aactaatcaa agcacagcct tggggaacag
    52741 tcctttattt tcaatcaaca gctgtttaga aaatggttta aaaagatagg atggttgaga
    52801 tgataaaaaa cggtttattt ttatgtagta ttggttcttt gatcgtgttg tatagtttga
    52861 atccacattc gcaaaggtat gatttgtata gtatggtcac aggtcttttt ctaacaggat
    52921 taggaatcta tttcttcatg aaggggaaaa aaagagaaga agcaaaggag aaaaataact
    52981 aatggaaatc acgaaaaaat taaatattcc agcatcgttt ttttacgata aagttatgga
    53041 ctctgtcctt ttcgatgtac gtaaagcgac cggcaaatca gtaactagaa aacagctgaa
    53101 aaatttggag tacgtcaaac aattctcgca aaataaccgg gctcgcataa aaatcgaaga
    53161 agtcattgaa aaccagtctt ataagtttcg tacatcgaca acgaagaatg aattcgtcgt
    53221 tcattatcaa atcaaaccat tggacgattc gacctgtgag gttcattaca cagaaaaaat
    53281 ggagtcttat ggcatgcttc aaaagttgaa tgatatgctt ttaggaacaa tcttgatgta
    53341 ttttaaacgg agacgtttca aaaaaatgct agatatgatc gaagcaacgt attaaatcaa
    53401 tgaaacatgg tactatttag ttattgaaaa atgaagtaag ctagaaggaa tttttcatag
    53461 gaagatagat ctttcctgcg aatcctcact agcttttttg taggaagggg cagcaaaatg
    53521 acaaacaaag taagagagct aggtgtatct tcgattgagg aaatgtttga tttagccact
    53581 tatgcattta atggggcaga tacacctgaa aggcgagaac gttttcgtac acttgcggag
    53641 aattcttgga attacggatt ttttgatgag gaggatcggc taacaagtca agtcatggcg
    53701 accccttttc cagtgaattt ttacggacaa gagtacctaa tggccggcat tggctatgtt
    53761 gcttcttatc cagaagcaag gggacaaggc gggatcaatc agatcatgga aaagatcctt
    53821 gaagattgcc gtgatcgtaa agtaagctta tcttatctgg cgccattctc ctatcccttc
    53881 tatcggcgct atggctatga acaatcattt gataaaatca gttatcaact aaacagtcgt
    53941 gatgtgccgt ttatcaaaaa aaaatctgga aaaataaagc gtatggattt tgaagatgca
    54001 aaagctgcaa tcagaagaat ttatgaacag atgccggaaa accaacgagc tggtctgaag
    54061 cgcgaagact ggtggtatac gtataaattc aagcaaaaag gcaattacca gtttgctttg
    54121 cattttgatg ataatgagga agcaacggga tatatcgttt atcagcttgc aacgtcttcc
    54181 tttattattg cggagtgggg atttttggat catgatgctt tttgcagcct tgttcgcttc
    54241 gtaagttctc acaatggtgc ttttgaaaca tttgaatatt catgcggcta cggcggtaat
    54301 gatcaaaatt atctattaga aaatccattt gcttctgtaa tgatcacacc ttatatgatg
    54361 gcgaggatcg tagatatttc tttgtttttg gaaaattatc catttaaaaa gagacaagct
    54421 gcatttgccc tacaaataga agaagatcat tatgcgaaat ggaatgaagg aatcgtggaa
    54481 gtaacaatcg aaaatggaaa aaatcatctc tcaaaagtag aacaaacaaa actcccaatc
    54541 gttcgaggaa gtatccaaca tatcacgcag ctattattag ggtatcgtaa agtggaagag
    54601 ttggtattcc aagaaagaat acaggtgaca aaagaatatg aagaaacgtt aagctcaatc
    54661 ttgccacagc aacagccaat tcttaatgac tatttttgat aaaaaacggg tgatgacaat
    54721 atttttgtca ccaccctttt gtttttcatt tggcatttga aggaaaatgt taaagtaaat
    54781 cctctttttt cgtaaatttg aattttttgt tttcttttgt tttaggtgtt ttagggtttt
    54841 cttttttgtg gtcttggatt gcttctttcg taatcttttc ccgatttttt ttagatgaat
    54901 ctaagcgttc tgcgtatact ttttcttttg gcttatcatt cattggaaag gacctcccta
    54961 cattatttca ttctacctct agtttatcat ctgttaagga ctagtggaaa cgttttgcct
    55021 aacttataag ctgatttttt tagcagataa gcttgcaagt aaaaaacaaa caagctacaa
    55081 ttgatttatt gaaattgttc ctgtagctca gtaggataga gcaatcgcct cctaagcgat
    55141 aggtcgctgg ttcaattcca gtcaggaacg tataaataac ccctgtatcc tttgcggtac
    55201 aggggtttgt tttgtatttg tgccaaaaat gtgccaaact agttatcatt tttatataaa
    55261 ctatcgaaca cagtattgat tttttcttta tcctgatctt caagctctcg aacaatatga
    55321 agataagtag acattgtagt ttctaaactg ctatgtccta aacgtttcga tacgctaagt
    55381 atatttactc cttgataaag taaaatagat gcatgtgtat gccttagtcc atgcaaagta
    55441 aattgctttt caatccctaa ttcttttaat tttttgcgca aatatttgct gacggcgttt
    55501 gatgaaacta atccattttt taagttgaaa aaaacaaaat tatgagggtt tttgatttca
    55561 aaattctcga ataattcttt ttgattgatt ttaaactttt ttaagagatc aatcgtatgt
    55621 ttatcaatgg aaattttcct atttgaagtt tcgttttttg tttttcccca ttcatttaat
    55681 ttataattcc atgttttatt aatagtgatg atttgatctt cgaaatcgat atcttcccat
    55741 gttaatccta gaagttcagc aaatcgcatt ccagtggcac cagcaactaa cacgagcata
    55801 ggagaagagt actgcgcaga aaggttttct tctacgagtt tcattaaaga tttaaattga
    55861 tcataatcta aatatttatc ttcttttggc ttcaatgaat cttttccttt gattactgct
    55921 tttctagttg gatcaaacgg aattaatcct tcttctacgg catctttcaa cgacgctcta
    55981 atatggt
//
LOCUS       DAXWOO010000002        54044 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.002, whole genome shotgun sequence.
ACCESSION   DAXWOO010000002 DAXWOO010000000
VERSION     DAXWOO010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54044
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.002"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(<1..1299)
                     /gene="ltrA"
                     /locus_tag="ACGE70_000060"
     CDS             complement(<1..1299)
                     /gene="ltrA"
                     /locus_tag="ACGE70_000060"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFF8691734.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHI"
     gene            complement(1868..2245)
                     /locus_tag="ACGE70_000061"
     CDS             complement(1868..2245)
                     /locus_tag="ACGE70_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8691735.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            2501..3229
                     /gene="purC"
                     /locus_tag="ACGE70_000062"
     CDS             2501..3229
                     /gene="purC"
                     /locus_tag="ACGE70_000062"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFF8691736.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            3229..3483
                     /gene="purS"
                     /locus_tag="ACGE70_000063"
     CDS             3229..3483
                     /gene="purS"
                     /locus_tag="ACGE70_000063"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFF8691737.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            3485..4156
                     /gene="purQ"
                     /locus_tag="ACGE70_000064"
     CDS             3485..4156
                     /gene="purQ"
                     /locus_tag="ACGE70_000064"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFF8691738.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            4157..6379
                     /gene="purL"
                     /locus_tag="ACGE70_000065"
     CDS             4157..6379
                     /gene="purL"
                     /locus_tag="ACGE70_000065"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFF8691739.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            6364..7803
                     /gene="purF"
                     /locus_tag="ACGE70_000066"
     CDS             6364..7803
                     /gene="purF"
                     /locus_tag="ACGE70_000066"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFF8691740.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            7826..8878
                     /gene="purM"
                     /locus_tag="ACGE70_000067"
     CDS             7826..8878
                     /gene="purM"
                     /locus_tag="ACGE70_000067"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFF8691741.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            8875..9453
                     /gene="purN"
                     /locus_tag="ACGE70_000068"
     CDS             8875..9453
                     /gene="purN"
                     /locus_tag="ACGE70_000068"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFF8691742.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            9450..10985
                     /gene="purH"
                     /locus_tag="ACGE70_000069"
     CDS             9450..10985
                     /gene="purH"
                     /locus_tag="ACGE70_000069"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFF8691743.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            11001..12275
                     /gene="purD"
                     /locus_tag="ACGE70_000070"
     CDS             11001..12275
                     /gene="purD"
                     /locus_tag="ACGE70_000070"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFF8691744.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            12397..13311
                     /locus_tag="ACGE70_000071"
     CDS             12397..13311
                     /locus_tag="ACGE70_000071"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFF8691745.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(13444..15009)
                     /locus_tag="ACGE70_000072"
     CDS             complement(13444..15009)
                     /locus_tag="ACGE70_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8691746.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(15002..16453)
                     /locus_tag="ACGE70_000073"
     CDS             complement(15002..16453)
                     /locus_tag="ACGE70_000073"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFF8691747.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(16467..17483)
                     /locus_tag="ACGE70_000074"
     CDS             complement(16467..17483)
                     /locus_tag="ACGE70_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFF8691748.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            17703..18791
                     /gene="chvE"
                     /locus_tag="ACGE70_000075"
     CDS             17703..18791
                     /gene="chvE"
                     /locus_tag="ACGE70_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFF8691749.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            18845..20371
                     /gene="mmsA"
                     /locus_tag="ACGE70_000076"
     CDS             18845..20371
                     /gene="mmsA"
                     /locus_tag="ACGE70_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFF8691750.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            20373..21578
                     /gene="mmsB"
                     /locus_tag="ACGE70_000077"
     CDS             20373..21578
                     /gene="mmsB"
                     /locus_tag="ACGE70_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFF8691751.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAVRLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            21835..22812
                     /gene="guaC"
                     /locus_tag="ACGE70_000078"
     CDS             21835..22812
                     /gene="guaC"
                     /locus_tag="ACGE70_000078"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFF8691752.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            23076..24500
                     /locus_tag="ACGE70_000079"
     CDS             23076..24500
                     /locus_tag="ACGE70_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFF8691753.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(24542..24799)
                     /locus_tag="ACGE70_000080"
     CDS             complement(24542..24799)
                     /locus_tag="ACGE70_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8691754.1"
                     /translation="MPIRILTALVNKITIRKAFYISSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            24961..26070
                     /gene="pdhA"
                     /locus_tag="ACGE70_000081"
     CDS             24961..26070
                     /gene="pdhA"
                     /locus_tag="ACGE70_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFF8691755.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            26073..27050
                     /locus_tag="ACGE70_000082"
     CDS             26073..27050
                     /locus_tag="ACGE70_000082"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFF8691756.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            27075..28718
                     /locus_tag="ACGE70_000083"
     CDS             27075..28718
                     /locus_tag="ACGE70_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFF8691757.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEIQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            28726..30132
                     /gene="lpdA"
                     /locus_tag="ACGE70_000084"
     CDS             28726..30132
                     /gene="lpdA"
                     /locus_tag="ACGE70_000084"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFF8691758.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            30294..30569
                     /locus_tag="ACGE70_000085"
     CDS             30294..30569
                     /locus_tag="ACGE70_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFF8691759.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            30575..31342
                     /locus_tag="ACGE70_000086"
     CDS             30575..31342
                     /locus_tag="ACGE70_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFF8691760.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            31510..33339
                     /gene="typA"
                     /locus_tag="ACGE70_000087"
     CDS             31510..33339
                     /gene="typA"
                     /locus_tag="ACGE70_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFF8691761.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVRCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            33600..35372
                     /gene="recQ"
                     /locus_tag="ACGE70_000088"
     CDS             33600..35372
                     /gene="recQ"
                     /locus_tag="ACGE70_000088"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFF8691762.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            35486..35788
                     /locus_tag="ACGE70_000089"
     CDS             35486..35788
                     /locus_tag="ACGE70_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFF8691763.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            35791..36954
                     /locus_tag="ACGE70_000090"
     CDS             35791..36954
                     /locus_tag="ACGE70_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFF8691764.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            36980..40408
                     /locus_tag="ACGE70_000091"
     CDS             36980..40408
                     /locus_tag="ACGE70_000091"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFF8691765.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            40429..41553
                     /locus_tag="ACGE70_000092"
     CDS             40429..41553
                     /locus_tag="ACGE70_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFF8691766.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            41534..41980
                     /locus_tag="ACGE70_000093"
     CDS             41534..41980
                     /locus_tag="ACGE70_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFF8691767.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            41964..42302
                     /locus_tag="ACGE70_000094"
     CDS             41964..42302
                     /locus_tag="ACGE70_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFF8691768.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            42421..42981
                     /gene="rsmD"
                     /locus_tag="ACGE70_000095"
     CDS             42421..42981
                     /gene="rsmD"
                     /locus_tag="ACGE70_000095"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFF8691769.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTIDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            42978..43469
                     /gene="coaD"
                     /locus_tag="ACGE70_000096"
     CDS             42978..43469
                     /gene="coaD"
                     /locus_tag="ACGE70_000096"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFF8691770.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            43459..44502
                     /locus_tag="ACGE70_000097"
     CDS             43459..44502
                     /locus_tag="ACGE70_000097"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFF8691771.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            44661..45389
                     /locus_tag="ACGE70_000098"
     CDS             44661..45389
                     /locus_tag="ACGE70_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFF8691772.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            45505..45750
                     /locus_tag="ACGE70_000099"
     CDS             45505..45750
                     /locus_tag="ACGE70_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFF8691773.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            45747..45965
                     /locus_tag="ACGE70_000100"
     CDS             45747..45965
                     /locus_tag="ACGE70_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFF8691774.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            46101..46640
                     /locus_tag="ACGE70_000101"
     CDS             46101..46640
                     /locus_tag="ACGE70_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFF8691775.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            46633..48906
                     /locus_tag="ACGE70_000102"
     CDS             46633..48906
                     /locus_tag="ACGE70_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFF8691776.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            48944..49990
                     /gene="holA"
                     /locus_tag="ACGE70_000103"
     CDS             48944..49990
                     /gene="holA"
                     /locus_tag="ACGE70_000103"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFF8691777.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(50079..50333)
                     /gene="rpsT"
                     /locus_tag="ACGE70_000104"
     CDS             complement(50079..50333)
                     /gene="rpsT"
                     /locus_tag="ACGE70_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFF8691778.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(50733..51002)
                     /locus_tag="ACGE70_000105"
     CDS             complement(50733..51002)
                     /locus_tag="ACGE70_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8691779.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(51264..51392)
                     /locus_tag="ACGE70_000106"
     CDS             complement(51264..51392)
                     /locus_tag="ACGE70_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8691780.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            complement(51472..51927)
                     /locus_tag="ACGE70_000107"
     CDS             complement(51472..51927)
                     /locus_tag="ACGE70_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFF8691781.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(52065..53993)
                     /locus_tag="ACGE70_000108"
     CDS             complement(52065..53993)
                     /locus_tag="ACGE70_000108"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFF8691782.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
BASE COUNT        17571 a         9413 c        11722 g        15338 t
ORIGIN      
        1 aatatgactt ttggagactg accaaatacc ttttcttgta tttgaccatt catacgcttt
       61 cgctttatcg atccctaaac gaatcagatt ttttcttttg gttcttggat tcttccattg
      121 tttccaaata tattgtctga ttcttcgctt caaccaaccg tttaactccc tcatgaattc
      181 tttcatctca ccaattccat agtagttgat ccaaccagtc attagttgct tgatttcttt
      241 taaaataagt tggatacttc ttccacggtt tcttttagtg agttgtttga gcttgctttt
      301 cacacgctgt tttgcttttg cgtgagggcg aattttaata cctttctttg ttggcagaat
      361 gcagaatcca agaaactttc gtttcaatgg acttcctacc gcactctttt ctcgatttac
      421 cgtcaacttt agttgttctt cgagaaattg aatggatttc ttcatcacac gctgtcctgc
      481 tcgtttgctt ctaacataga tattgcagtc atcggcgtat cgaacaaatt tatgtcctag
      541 attttctagc acttgatcaa attcgttcag gtagatgtta cttaacaggg gagagagatt
      601 gcctccttgg ggcgttcctt cttcagttgt tgtgatgagt ccattttcca ttacaccact
      661 cattaagtat tgacggatca gttttaggac ttgtttgtcc tttactttcc gctcaatgaa
      721 atacatcagc ttatcgtgat ttacggtgtc aaagtaggct ttcatatcaa tgtccaccac
      781 atatcggtaa ccttgttcat aataagattt tgctttcttc actgcgtcat gtgcgctgcg
      841 atttgggcga aacccataac tattatctga gaattctggt tcaaatagcg gttgtaacac
      901 ttgattgatt gcctgttgaa gcatacgatc aataaccgtg ggaattccta gttttcttac
      961 accgccgttt ggtttcggta tttctactcg caaaacaggc tgaggtttat agttcccttg
     1021 gcgtaaattg gatagtaatt cctcgcggtg ctctttcaag taaggaagca gttgatcgac
     1081 ggtcatccca tctgcaccag ccgccccttt atttctgact acttgaagat acgcttgatt
     1141 gagattattc cgatctagaa ttctcccaaa cagattttct acaccatctt gtttcctctg
     1201 ttcaccgcaa gaagtgctgc gcacttcatc atacccttcg tgttccacac tagctctttg
     1261 atgatagcca agttttcttg ttttctgcgg tcgtcgcatc ttctcacacc tcctaaatta
     1321 ttcaagattc ttattgttca gtccttcgta acggtcagtg tcgttactac tatgacctcg
     1381 gctgacttct catgattcac catcatgtcg ccatgatcgt tgtgctggtt tgttttcatt
     1441 caacgcccac aacaccctca tgagatctcc cccggtaaga gtgaaaactt tcctcccatg
     1501 taactgctag atttactgta taggtttcgg gcagtattgg actttgtctt gtgatgaaga
     1561 cttatccgac ctaattcagc cttgtatcta gtttctgttc gtcagttcag gagtttgcgt
     1621 ccgacttcct tcagccacaa cctcacgatt gccgccttgt ctttcgctaa cagttcctac
     1681 tgccaagcct gtagtggact ttcaccacca agttttcacc catgcagggc gcacaccaaa
     1741 aaagaagcag atccgttagc ggttctgctt cttttagttt attatatttt cttatcaata
     1801 ataggaacct gattttaaaa gcggctacct ctttaccgtg cctctttaca tacaaaaaat
     1861 agaggaatta agcgtaaatg gcttggctat tataactaaa atgctggtta tggagatgtt
     1921 gcagcaagtt ttcgagaaaa gcgtattcca tctcttgtcc attgaataag atctggatac
     1981 gataatgatt tttgaaacgc ttctgttcat gaatctgact gattgaaagt tcgcctttgt
     2041 gttctgtctg ataaagaaaa gtccttttac ggcattcagc acagcgcttg gttgctcggc
     2101 ggatttttcg gaatgtataa aaactagtat ctttattgca taaataaacg tctttgatcg
     2161 acagcaaatc attatataga caggaacgtt cttcagctgt taattctgat aaaaaaagct
     2221 gaccttgttt tattgcatct tccatcggca agtgcctcct cgaacccatt atccaaattt
     2281 ttagttaatt ctaatatacc atcattaagt ataaatgtaa acgcttgttg atggtataag
     2341 attttcgttt ctgataaaac acgaacatta aaagaatcta atgccttaaa tgtacatatt
     2401 tttgttgatt tataaaaaga tgattgctaa aatttaaagt gaaaaaacga acaaaaaacg
     2461 atgtatttgc ttttaatgta aataaaaagg agagacgtac atggagaaga aagaactttt
     2521 gtatgaagga aaagccaaaa aaatatatct tacttctgat gagaacttga tttggataga
     2581 atatttagat caagcaactg cgttgaatgg cgcgaggaaa gaccagataa gtggaaaagg
     2641 agaattgaat aatcaaatca caagtgcaat tttccgttat ctgactcaaa gaggaataaa
     2701 aaatcatttt attgaacaat tgtctaaaac cgaacaatta gcaaaaaaag tacaaattat
     2761 ccctctggaa gtggttatcc gaaatattgc agcaggaagt ttctcaaaac gtctaggtgt
     2821 tcccgaaggt aaagaattca tcaaaccaat cattgaattc tactataagg atgaccagtt
     2881 ggatgatcca tttatcaatg aagagcatat ctatttctta caacttgcaa agccggaaga
     2941 aatcagtgag ctgaaagccc aagcgttggc tattaatgaa attttgaccg aattatttgc
     3001 taaggtcggt ttggttttag tagatttcaa gctagaattt ggccggtcga atggacaaat
     3061 tttgcttgcg gatgaagtaa caccggatac ttgccgatta tgggatgaac gtacgaatga
     3121 acatatggac aaagacgttt accgcagaga tctaggtgat ttgatcccag tctatcaaga
     3181 agtatataac cgtctatcgg aactaactga agaaaaggag cgagtatgat gtatttcgta
     3241 aaagtatatg taacctataa agattcagtg ttagatcctc aaggggaggc agtaaaaaaa
     3301 gcagtccatc gtatgggata tgaaacaatc gaagatatcc gaataggaaa atactttgag
     3361 atacaacttg ctgatgcaga agaaccaatc gatacagtga ttgaaacgat ttgtgacaag
     3421 ctgcttgcaa atgtaaatat ggaaacatac cgatatgaaa tcaaaaaaat ggaggaagta
     3481 taacatgaaa tttgcggtca tcgtttttcc aggttcaaat tgtgacgcag atatgttatg
     3541 ggcagttcgc gagatcatgg gcgctgatgc cgaatttgta cggcatgatg agtcctcttt
     3601 agaaggattt gatggggttt tactgccggg aggattctcc tatggtgact atttgagatg
     3661 cggtgcgatt gctcggtttt ctacaatcat ggaagaagtg attcgttttg ctgaagaagg
     3721 gaaaccagta ttcggtacat gcaatggatt ccagatattg acagaagcag gacttttgcc
     3781 aggtgtactg agacgaaatg agtctttgca ttttatttgc aaaactgtac cattgaaagt
     3841 caaccccaag acggccttta cttcagagta tgaagccgac gaaataatct atcttccagt
     3901 tgctcatggg gaaggaaatt atttctgtga cgaagaaaca cttcaagaac taaaaaaaaa
     3961 taatcagatt gcctttactt ataatgacaa catcaacggc agcgtggaaa acattgcagg
     4021 gattacgaac aaagcaggga atgtgttagg aatgatgcct catccagaac gtgcagtaga
     4081 agacttgcta ggatcagaag atgggaaacg gttctttgct tccattttaa aaaacttcgg
     4141 aaaggtgacc gtatagatga aaacattgaa agaaccgaca gctgaagaga tcaagaaaaa
     4201 aagaatctat gccgaatggg gattgactga tgaagaatat cgaatgatcg aagaagatat
     4261 cttaggaaga atgcctaatt atacggaaac tggactgttt tctgttatgt ggagcgaaca
     4321 ttgttcctat aaaaattcta aaccggttct acgcaaattt cctactagtg gacctcatgt
     4381 gttgcaaggc cctggagaag gcgcaggcat cgtcgacatc ggtgatggac tagcagttgt
     4441 cttcaaagca gaaagtcaca atcatccttc agccgtggag ccttatgaag gagctgcgac
     4501 aggagttggc ggaatcatca gagatatttt cagcatgggg gctcgtccaa ttgcgttgct
     4561 agattcattg aggttcggcg aattagataa tccaagaacg aaatatttac tagaagaagt
     4621 agttgcaggt atcggcggat acggcaattg tatcggcata ccgactgtag gcggcgaaat
     4681 tgcatttgat ccttgttatg aaggaaaccc attagtcaat gcaatgtgcg taggtttgat
     4741 tgaacacaaa catatccaga aagggcaagc cgctggcgtc ggtaattcga tcatgtatgt
     4801 aggtgcaaaa acaggtcgcg acggtatcca cggagcaact tttgcctcag aagaatttaa
     4861 tcaagaagaa gaacagcaac gttctgctgt acaagtaggc gatcctttta tggaaaagct
     4921 gctgcttgaa gcgtgtttgg aattaatctt gcatcattcg gattctctaa taggtataca
     4981 ggatatggga gcagcaggac ttgtttcatc tagtgcggaa atggcttcaa aagctggctc
     5041 aggacttata ttgacattag atgaagtccc acaaagagaa actgggatga caccttatga
     5101 aatgatgtta tcggaatcac aagaacggat gttgatttgt gttaaatcag gagaggaaga
     5161 aaaaatccaa gaactatttc agaagtatga tctagatgca gtcactatcg gtaaagtaac
     5221 agacgacggg caatatcggc tatatcatca aggagaagaa gtagcaaatc tgccagtcga
     5281 tgcattagct gaagatgcac cagtttatca taaagaaatg aaagaaccag ctcgaatcgt
     5341 agaatttcag cagttagctc cttatcagcc agttatcgaa gaacctggac aagtattgct
     5401 tgatctgctg cagcaaccga cgattgcttc aaaacgttca atctacgaaa cttatgattc
     5461 acaagtacaa accaatacag ttgtccgtcc aggaagcgat gctgctgtga tgagggttag
     5521 agggacgaat aaagcgttag ctatgaccac agactgcaac gcgcgctatc tgtatttgga
     5581 tccaaaaatc ggaggacaaa tagcagtggc agaagctgct agaaatatta ttgctagcgg
     5641 tggtaagccc ttggcgatca ccgactgcct gaactatggt tctcctgata aaccagaagt
     5701 tttttgggaa ttatcgactt cagctgacgg aattgctgct gcgtgtgaaa cgttaggaac
     5761 tcctgttatt tcaggaaatg tctctttata taatgaaacg gatggtcaag caatctatcc
     5821 aacaccaatg attggtatgg tcggattgat tgaggatcat aaacatatca ctactcagga
     5881 attcaaaaaa tcaggggatc taatctatat attaggtaag acgtttgcag attttgatgg
     5941 cagtgaatta caaaagatgc agcttggaag aatcgaaggt gtgatccgaa actttgatct
     6001 atccattgaa aagagaaacc aagagttagt attgacagcc atccaaaacg gattgattga
     6061 aagtgctcat gactgttcag aaggtggctt ggcaattgca ctggcagaat cagctttcaa
     6121 acatcagcta gggatatctg tacagtttga attgtcctct gctcaattat ttgcagaaac
     6181 tcaatcaaga tttgtgttga cagtcgcacc ggaaaataag acacgattcg aagaaatgat
     6241 gggagatgct gctgttttag caggtaaggt gacggatgaa gcgataatcg aaatttcagc
     6301 aactgacggt cagatcaaaa tagaaactgc agtagcaaga aagtgctggg aggatgcaat
     6361 cgtatgtctt atgaagtgaa gagtttaaat gaagaatgcg ggatcttcgg tgtttggggt
     6421 catccagacg ccgctagtgt ggcatacttc gggctccata gtcttcagca tcgaggacaa
     6481 gaaggtgcag gtatcgtgtc caatgatcga ggaaagctta aggggcatcg agatttgggg
     6541 ctgctttctg aggtattcaa agatcaaaga aaattgcagc agttaacagg agaagctgca
     6601 atcggacatg tacgttatgc aacagcaggc aatggaagtg tggacaatat tcagcctttt
     6661 ctattcaaat ttcatgatca gtcgatcggc ttagcgcata atggtaactt gatcaatgct
     6721 cgttccctta gaaaagagtt agaggaaaat ggggcgatct tccattctaa ctccgatacg
     6781 gagatattga tgcatttgat ccgacgaagt gaagaaacag attttttaaa taaattaaaa
     6841 agtgctttga atgaagtcaa aggtggcttt gcctatttat taatgacaga aacggcaatg
     6901 attgccgctt tggacccaaa tggttttcgt ccattagcaa tcggacaaat gaaaaacgga
     6961 gcatacgtga ttgcctcgga aacttgtgca ttagaagtta ttggggcacg ttttataaga
     7021 gatgtccagc ctggtgagat agtgatcatc aatgacgaag gaatccagat tgattgcttt
     7081 acgaaggaca cgcagctttc tatttgttcg atggaatata tctattttgc cagaccagat
     7141 tcaaacatcg cagggatcaa tgtgcatact gcacggaaaa atatgggacg gaatttagcg
     7201 aaagaatcgc cagtaaaagc agatatggtc gttggcgtac ctaattcttc tttgtcagcc
     7261 gcaagcggtt atgcggaagc tagtggaatc ccttatgaaa tcggattagt aaaaaatcaa
     7321 tacattgccc gaacatttat ccagccaacg cctgagctaa gagagcaagg tgttcgaatg
     7381 aaattatctg ctgtacgagg agtagtggaa ggtaaaaagg tgattatggt cgatgattca
     7441 attgttcggg gtacgacaag cagacggatc gtccatcttt tgaaagaagc aggggcaaaa
     7501 gaagtccatg tgaggattgc ttctcctcct ttgaaatatc catgttttta cgggattgat
     7561 attcaaacgc gtaaagaact cattgcggca aatcacacga ttgatgaaat caaagaatgt
     7621 atcggtgcag attctttagg ttttttaagt gaagagggtc tgatcgaatc gatcggtctg
     7681 gaaaaagatg ctccgtattc aggattgtgt atggcttatt tcaacggaga ttatccgacg
     7741 ccgttatatg actatgaaga aaaatatcag gaatcactaa aagaaaaagt atcgttcttt
     7801 taaaagcaga ccaagaaggg agtatatgaa ttcaatggca aatgcatacg caaaagctgg
     7861 cgtagatgtc gaagctggat atgaagtagt agaacggatc aagaagcatg tcaaaaaaac
     7921 ggaacgttta ggtatgatgg gcgctatagg aggattcggc ggttgttttg atttatcggt
     7981 ctttcaatta aaagagccag tccttgtctc ggggaccgat ggcgtgggga cgaaattagc
     8041 agtggcaatc gaagcagaca aacacgatac aatcgggatc gactgcgtag cgatgtgtgt
     8101 caatgatatc ttagcacaag gcgcagagcc gttatatttc ttagactact tagcaacggg
     8161 gaaaaatgat ccgttacgtt tggaaaatgt agtaaaaggt gtagcagatg gatgtgttca
     8221 aggaaatatg gcgctgattg gcggagaaac agctgaaatg cctggtatgt actcaggtaa
     8281 tgattatgat ctagccggat ttgctgtagg gatagctgaa aaatctcaat tattgacagg
     8341 agaaacgatc aacccaggag atgtgttgct tggaatagca tctagcggga ttcattcaaa
     8401 tgggtactct cttgttcgca aaattttctt tgagcagcag cagttttctt tatcatcaaa
     8461 actgccagaa ttgaaaaaaa cacttggcga agaattgctt gagccaacga aaatctatgt
     8521 ttcttccttg cttccgctga tcaaagaaaa gaaaataaaa ggagctgcac atatcactgg
     8581 tggagggttt gtagaaaata tcccgcgaat gttaccagaa tctatggctg cacaaatcaa
     8641 acttggctca tggccgattc ttccaatatt cggctgttta caaaaatatg gtgaaattcc
     8701 tgagatggag atgtatgaaa tcttcaatat gggcatcggt atggtcctgg cagtccaaga
     8761 atcgaaagta aaagaaatag tacacgaaat tgagcaagct ggcgaaaaag tttatgaaat
     8821 cgggaaagtg atcgccaaag aaaaacatcg tgtggtgttt ataggagaag aacgatgaga
     8881 atcgctgttt ttgcttctgg aaacggaagc aactttcaag cgctagccga ttatctatcg
     8941 aaaaaaggat tggaatcttc gattgactgg ttgttttgtg atcagcctga agcatatgtt
     9001 ttgaaacgtg caactgcgtt gagtgtaccg gcagattgtt tttcacctaa agaatttgat
     9061 tcaaaaaaag agtatgaaga agcaattttg cataaattga aagaaaaaaa gatcgatttg
     9121 atcgtattag ctggatatat gcgaatcatt ggaccggtat tgctgaaaaa ttatgataaa
     9181 agaatcatca atatccatcc gtctctttta cctgccttcc ctggtttgca tggcatacgt
     9241 gatgcctttg aagctggagt aaaagaaacg ggtgtcacaa ttcattatat cgaccaaggt
     9301 gtagatactg gaccaatcat ccggcaggaa aaagtgagaa ttgaacagga ggacacgttc
     9361 gattctttag aagaaaaaat ccatagagta gagcatcgaa tctatccaga agtgattagt
     9421 gaaattattg aaaatggagg ctatctgaaa tgacaagagc attgatcagt gtatcagata
     9481 aaactgggat tgtagcattc gcccaaggtt tgattgaaca aggagtagaa atcatttcaa
     9541 caggaggaac aaagcatgcg ttggataaag ctgggatcaa aacgctttcg atcgaagaag
     9601 tgacagggtt tcctgaaatg atggatggcc gtgtcaaaac acttcatcct aaaatccatg
     9661 gtggtctttt aggaagacgc gatctcccta gccatatgga ggcaatggaa acggaaaata
     9721 tccaaccaat cgactttgtt tgtgtcaatt tatatccttt caaagaaacg attatgaaac
     9781 cagatgtcga aatagcagaa gctattgaaa acattgatat cggtggtcct agtatgctcc
     9841 gtagtgcagc aaaaaaccat gaatttgtca cctctgttgt tgatccatct gactatgaag
     9901 aagtactttc agaattacgt cagtatgggc atacttcact ttctttcaga caaaaactag
     9961 cggcaaaagt ctttcgtcat actgcggcat atgatgcact gatcgctcaa tacttaacgg
    10021 attgggtgga agaaaaagaa ccggaaaaat tgacacttac gtatgaacga aaacaaacgc
    10081 ttcgttacgg tgaaaatagc catcaagccg ctgcctttta tcagtccgct cttctggaaa
    10141 aatattcgat cgcagcagca aaacaactcc atgggaaaga actttcttat aataatatcc
    10201 gagatgcaga tgctgcgcta cggatcatga aggaatatga cgagccaacg gtagtcgcct
    10261 tgaaacacat gaatccttgt ggtattggga cagcatctac gattttagaa gcgtgggaag
    10321 cagcgtatga agcagatcct gtttcaatct ttggtgggat catcgttttg aatcgagaag
    10381 tagatctgcc aacagcagaa cagatgcata agctgtttct agaaatcatc attgcccctt
    10441 cttatacaca agaagctttg gaattactgc aaaaaaagaa aaacatccga ttactgacag
    10501 tagactttgc gcaggaagaa aatcaaaatc gagaagaaac ggtctcagtc ttaggtggac
    10561 ttcttgtaca agatcaagat ctggcaatag aaaatgaaga agagtggaaa gtcgtgacaa
    10621 aacgtaagcc gactccagca gaagcaaaag cattagcgtt tgcttggaga gctgtcaaac
    10681 atgtgaaatc taatgcgatc gtactggcaa acgaacgtca gacagtagga atcggagccg
    10741 ggcagatgaa tcgagtcggt tcagtaaaaa ttgcgattga acaagcgcaa gagaaaatgg
    10801 aaggtgcagt tttagctagc gatgcttatt ttccaatgga tgattcggtt gaatatgcag
    10861 ccaaacatgg tataaaagca atcattcagc caggaggaag catcaaagac caagattcta
    10921 ttgatatggc gaataaatac gatctagcaa tggtctttac tgatgttcgg cattttagac
    10981 attagagagg agtcggataa atgaacattt tggtgatcgg tgcaggtggt cgtgaacatg
    11041 caataagtaa aaaactttta gccagtccga aggtggatac ggtgtattgt gcgccaggaa
    11101 atccggggat gacaaaagat ggtatcaatt gtgtcgctat cagtgagcga gatcattcag
    11161 cgttaatcca ttttgttaag aaagaggcta ttgattggag tatcgttggt cctgaagttc
    11221 ctttgttaaa tgggatcgtc gatgatttta tggcggaagg attgaaaatc ttcggtccga
    11281 caaaagcagc agctctgatc gaaggttcga aaagttttgc taaacaatta atggatactt
    11341 atcaaatccc gacagctaaa tccagaagtt ttgaatgtta cgaaaaagca gcagcgtata
    11401 tcaaagaaaa tggtgcgcca atcgtagtga aagcggacgg cttagcagct gggaaaggtg
    11461 tagtcgtggc taccacagtg aaggaagcgc tggatgcggc agaacaaatg ttgaaccagc
    11521 atcgatttgg ggatagtagc aaaaaaatcg tcgtagaaga gtttttggca ggagaagaat
    11581 tctccctttt ggcgtttgtc cgtaatacca aggtgtatcc gatggtcatt tcgcaagatc
    11641 ataagcgggc atttgataac gataaagggc caaatacagg cggaatgggt gcttattcac
    11701 cagtaccgca aatttcaaaa aagatgattg aaaaagctgt ggaagaagtt ttaaaaccag
    11761 cggctttggg aatgaaagaa ctaggacgct ctttcacggg tgttttatat gcgggtttga
    11821 ttgctacgaa ggaaggacca aaagtgatcg aattcaatgc gcgtttcggt gatcctgaaa
    11881 cgcaagtcgt tctttctcgg ttgaagagtg actttgcgca agtaatcgat gatttattag
    11941 agaatcgtac agtagagtta aagtggcagc aagaaggata caatgtaggc gttgtggttg
    12001 cagctgccgg ttaccccgaa gaatatgaga caggaatggt tctaccagat tttctggaag
    12061 aagagctttc tgtttactat gctggagttt cctcaaataa cggcaaacta gttggagcag
    12121 gaggacgtct ttatttagta gaagcatacg gtgagacgat caaagaagca cagaaaaaaa
    12181 tttacaccgc gttaatggat aaaacgacag atggaacatt ttatcgtaca gatattggag
    12241 aaaaagctgc caatagcgaa ctgatcgtga agtaaatcag ctgcttccat ttataaaaaa
    12301 tacaacaaac ataatcggaa gcgccgattt tagtgaagcc ttttcataaa gagtgtggta
    12361 cacttttgtt gtgtatataa ataaggagga gttcagatga aaaaaattca attaggtact
    12421 tctgatcaga aagcagctgc agtgatctta ggctgtatgc gtatcaacaa tgcggaagat
    12481 ccagtaaaag tgattgagac agcttatgaa aacggcattg atttttttga ccatgcagat
    12541 atttatggaa acggtgaatg tgaagaaatc tttgcagatg ctctagcaaa aacatcaatc
    12601 aaacgagaag atctttttat tcaaacaaaa tgtggaatcg ttccaggtgt gatgtttgat
    12661 ttttctaaaa aacatatcat tgaatctgta gaaaaaagtt tgacccgtct gaaaatggat
    12721 tatgtggatg ctttacttct tcatcgtcca gatacattgg tagaaccaga ggaagtagct
    12781 gaagcgtttg atgaattgga acggtcagga aaagttcgtt attttggcgt cagcaatcaa
    12841 aaaccaatgc aaatcgaatt gctgaaaaaa tatgttcgtc aaccattagt agctaatcag
    12901 ctgcaatttg gcttgaaaca tactggaatg atcgatcaag ggattcatgt caatatgaca
    12961 gatgaagcaa gtatcgatca tgacggtagt gtacttgatt attcccgatt gcatgatatg
    13021 acgatccaag catggtcacc ataccagtat ggattttttg aaggtgtatt tatcgggaac
    13081 gaaaaattcc ctgaattaaa ccaaacatta gaagaactag cagctaaata tcaaacgaca
    13141 ccaactggct tggcttctgc ttggatttta cgccatccag ccaatatgca agtgatcgcc
    13201 ggtacgatga acgtagggcg aatcaaggaa atcgctcaag cttcagaaat taatatgtct
    13261 cgggaagatt ggtacgaatt gtaccgtgct gctggaaaca ttttacctta aagattgtca
    13321 taagcaggtc aaaagggcta taaattaaaa agacagaata acttgaaagc tgttttcagc
    13381 ttcgagatat tctgtctttt tttattgcaa atggaaagag tatcattgta cggatttcgt
    13441 ttattagtta tttttgtttt taaggaaaag ctgtgtccga tattcttttg gagaaatccc
    13501 ttgttttttc ttaaataaat aactgaaata gttagggtca ttataaccaa tttcaaaagc
    13561 aatatctgaa atacgatagt ctgattgctg caataatttt tttgctaatt cgattctttg
    13621 ttctgtcaga taatcgatga atgtttggtt aaacgattga gaaaaaatcg tactgaaatg
    13681 agagggactt aatgcaactt gttgagcgac ccaagttaag gacatatctg gatcggtaaa
    13741 attttctttg ataaaatcca atgcatggtt caaaattggc tgatatttgt tccttgaagg
    13801 ctgtgagggc agagcagtta agaaagaaat gaacatataa actgtcgaac ggtaaagcga
    13861 gtcgtcggcg ctgattgcta agcgttgact ggtggatgtg caatgaacca tttcttgcgg
    13921 gattttcttt tctttcaggc agacatgctg gagtagctta gtcaagacaa ataaacgata
    13981 taatcgtgtt cgagttggtg tgccttgtgg tttcgtcaag tgttcgatgt acttagatac
    14041 ttcgtttttc gataatgaag acaaatcaag tgagaggtca aagtaaaaga cagaatcaac
    14101 tggaagtttc gcttcttgaa tcaaatcctc atagctgata atttgttctt taagggaact
    14161 ggcggtttca agtatcatct gcgtgatctc aaaagatgca ggtaattcgc tgattctttc
    14221 tgcgacagga ccaatggcga cagaaaaatt atttttttgt cccttttcta attcatggac
    14281 caacgtttgg gctacctgat aactggtctc gatcacattt tcttcaagag aatcaactat
    14341 taaaaatttg atgaatcgag aggaaacagc agagaagatc acttgttgat tctccctaaa
    14401 taaaaacttc aaataatcac tgagatgagc gtaatcttca aagtctgtac tgacttggtt
    14461 tgctgctaat aatacggtca tttttttccc tgctaaagag cgattgaaag attcagcaag
    14521 tttcaatgtt tcactgatcg taagtgagcc tttgaacaaa ccgttcaaga aatgattttt
    14581 tttcaattcg aaaacaagat cagaagaagt gtgcgtttct ttttgctgca aatctaaagc
    14641 agttactgct ttttctattg cttgtttcaa ttcgctactt ttaatcggtt taagcagata
    14701 atcatctgcc tgtacctgga ttgctccttt catataatcg aaatcgtcga atccactaat
    14761 aaatatgaga cgagtccaag gaagtagttt ccttgcttct tttgaaaaag tcaacccgtc
    14821 catgaaaggc atttggatat cagtcagaat gatgtcgggc tgtaagtcga taattgaaga
    14881 cagtgccatc tcaccatctc cagcttcgcc gacataagtg attggataat aagaaccaag
    14941 gacagttatt tgttcccgca agttattgcg aatgaggtat tcgtcttcga tgataaaaac
    15001 tttatacatg tttgtttcct ccaattgttt tgggagatag agggatcatc agtgtgacag
    15061 ttgttccttc tcggtatttg ctgtcaatcg tgataggttt ttgatcattt ccgtaataaa
    15121 gaaggagacg cttgtttacg ttgtaaagtc cgtagccgat actgaaatct gaatcgatcc
    15181 ctttggctaa ctctttacgt atttctgcca gtttttctgc agggatgcca atcccgttgt
    15241 ctataacttt gaattggatc atctcatcaa ttttgcatgt ggtaatacga ataaatcctt
    15301 tgtgacggac gtacttcgtt ccatggtata cagcattttc aatcaatggc tgcaagatca
    15361 tttttaaaat agaaacagga aaaagtgttt ctggtatatc gatcttatag tcaagcatat
    15421 ggccgtatct tatttttaag atgactagat aatcattgat atggctaatt tctttttcaa
    15481 gaggaatcca gtcttgtcct ttgcttaaag acgtacgaaa aaagccggaa agtgcatggg
    15541 tcatagtcag agcttcttca aagcagtctt gttcgatcaa agaaataatg gcatccaaac
    15601 tgttataaat aaaatgggga gtaatctgag cttgcaatac acgaacttca ctttgtgcta
    15661 aatggtattg tttcaaagcg ttctcccgta aagactggtt tagatcttcc gccattttat
    15721 tcatgctagc ggtaagtgtt tgtaattcca gtgtttccgg cagggttaaa cgaaagccaa
    15781 ggttccctga agaaagttgt tctgacatct cgatcagctg attgatcggt tcttggacct
    15841 ttctctctaa aaagcgacga ttctttttcg catagaaaaa aatggtacta agaactccaa
    15901 gttccactat tgttaataga atgagcgagc ggacaagtgt cgtatttttt ttgcttgcta
    15961 agtcgatctc cacacgaaca aaatcttgta agatatctga cagcagttga gtgacagaat
    16021 ctacctgatt gatgatctct tcattggtat ccataaaatc ttttgccaag atattattga
    16081 tcagctcgtt ttgataatct tccagtgtat cgatcgtacg tagcgataca tctaacgtac
    16141 taatttcttt aagtgtattt gtattatttt tgatctctcg aatatcattc ctaagttctt
    16201 ctaggatatt ttcctcattg aactcatcta cggttacttg gccagaaatc aagtcccaca
    16261 tactttccaa aactttttga tcaaccttca aagcaattgc atctgctttt tggatatttg
    16321 cgatcgtctg ttgataaatc aaaagctgtt gcgaatataa cagtatgctc agtagcaaag
    16381 ggataacagc aaatagaagc atggctttgt ttgcttgggc aaaaatctgt tttatgctta
    16441 aattttttgt catgatactt ctccagtcaa taatttctgg ttgggatgtt agctatattc
    16501 tgctgggaaa agacagtctc ggaaataaaa atatctttag gtaatgtttt tcctgtttga
    16561 atatttccaa aataacgagt aacggtattg gctacatacc agccgacatc aggtttacac
    16621 tcgactacac aattgacgat tcctgagcgt agcttctcga tcatatcttc ttgtccatca
    16681 atagttacaa tgatcaaatc ttctccaggt ttgattgatg tttcttttat cgcagctaaa
    16741 gcaccgtgag tcatttcatc actatggctg tataatacat cgatttcgga taaatcattt
    16801 gtttctaaat atcgtttcat ctggtctttt cctttcatcc gaataaaatc accgacaatt
    16861 cggtgaacaa tagtcatcgg aggtttatgg tcgatggttc catctttttt tagggttggt
    16921 ttacgattga ttgattccaa aaatccttcg gagcgtagct tggtcggtgt ggagttctct
    16981 gaaccagcaa gttctaggat tctgatttcc tgtttctttg aatttgtgaa gtgattgctg
    17041 acaaataaac ccgcccgatt tccttctgct ttgaaactgg ggccaatgtg ggtgacgaaa
    17101 agctcgggat cattcgtttg aatatgtctg tcgacaagta tgacagggat ccctgctcgc
    17161 tttgcttctt ttaatacaga gtcccacccg gattcttgca aaggagtaaa aatgatcata
    17221 tccactttat aggcaatgaa cgtccgtata tcttggattt gccgttcctg attcatgtag
    17281 ccatttcggt agagaacttg gtaatcttct ttttcaagcg cagtgataat tgattcagta
    17341 tgctttttcc gccagctact ctccgttcct gattgcgaaa aaccaacgac tagtgggtca
    17401 ggtgcttcct ttgtattagg tgtacacccc atcatttggt aagcgattac aacaagggct
    17461 aaaaaaaata aaacacgttt cactttaaat cactccatct ggatttgtta gtactatctt
    17521 atcagataaa agggaaaatc aaagtcaaaa ggaatcaatc gaaaaaaatc caagaaactt
    17581 aaaaaaatcc aagacaaaaa aagtacacct aaaaaattta aagtaaatcc cataaaaaaa
    17641 tcaatgttaa cgctttcttt attagctata ctcggtgtat caattgaaat gggggataaa
    17701 aaatgaaaaa caaatttaga aagattgttg ggactatcgc tattttgacc gcaagcattc
    17761 tattaagtgc gtgtggcaat tcaggatctg cagatgatag tacggggtat gtaggaatcg
    17821 ctatgccgac caaatcagca gaaagatgga tcgctgatgg gaacaacatg gtctcggaat
    17881 tagaaaaact gggttataaa acagatcttc aatatggaga agataaagta gaaaatcaag
    17941 tagcacaaat agaaaatatg attacaaaag gggtagatac acttgtgatt gcttctatcg
    18001 atggttcggc tttaactgat gtgttagcta aagcaaaaga agcagatatc aaggtgatcg
    18061 cctatgatcg tttactgatg aattcagaaa atgtcgatta ttacgcgact tttgataatt
    18121 ttggagtagg tgtatcgcaa gcagcttata ttgaagagca cttaggattg aaagaaggaa
    18181 aaggaccatt cacgattgaa ttatttggtg gctcgcctga cgacaacaac gcactaatca
    18241 attacaacgg agtgatgtct gttttacaac catatatgga caacggtcag ttggtcgtgc
    18301 catctggaca gacgagtttc agtcaaatcg ctacgcttcg ctgggatggt tctacggcgc
    18361 aagcaaggat ggataatctg ttaagcgcaa attatactga ccaaacattg gatgctgttt
    18421 tgtcgccata tgatccaata agtttaggaa tcatttcgtc attaaaaggc gtggggtatg
    18481 ggagtgaaag taaaccttta cctgtgatta ctggacaaga tgcgacagtt gctggcgtta
    18541 aatcgatcat tgccggggaa caaacacaaa cgatctttaa agacacacgt atcttagcaa
    18601 aaaatacgat tgaaatgatc aaagcaatca gtgatggaga agaggttccg gtcaatgata
    18661 ctgaaactta tgataatggg gtgaaaactg tccctacgta tttagcaaat acagtatctg
    18721 tcgataaaga taattatcaa gcagagctga tcgacaccga ttattataaa gaatccgatc
    18781 tgaaaaatta agaggaacca aacaaggaac taaagagatc taagtcagag agaggataag
    18841 aattatggcg gattgtattt taaaaatggc agatatcacc aaaaaatttt ctggaatccg
    18901 agcgttagat tgcgtgaatc tgatgattga acgtggggaa atccatgcat tgtgcggaga
    18961 aaacggagcg ggaaaatcaa cattgatgaa tgtcttgagc ggcttgtatc catatggctc
    19021 ctatgacggg gaaatctttt atgatggaaa gctgtgtcaa tttaaagatt tgaaagatag
    19081 tgaagaaaaa ggcatcgtca tcattcatca agaattagca ctaagtcctt atctttcaat
    19141 aaaagagaat atttttctcg gaaatgaaca agcagaaaga ggcgtgattg attgggaact
    19201 gacagaaaag aaaacatatg aactgctgaa taaagtcggt ttgtctatcg acccgaatct
    19261 tctagtatcc cagattggcg tagggcaaca gcaactagtt gaaatagcta aagcattatc
    19321 aaaatccgtc cgtctgttga ttttggatga gcctaccgct gcattgaatg aagaagaaag
    19381 cgcgaatttg ctgagattga tccgagagtt gaaagagcaa ggtgtaacct ccatcattat
    19441 ttcacataag ctgaatgaaa tcgtggatat cgcagatcga atcaccattt tacgggatgg
    19501 acagacgatt gagacactag aaaatgaaat gatcagcgaa gagcatatta ttcgcggaat
    19561 ggtgggcaga gatttagttg atcgttatcc tgaaagaaaa ccggatattg gagaagttta
    19621 ttttaaagta gaagactgga cggtctatca ccctcttgat cacgagcgaa tcataaacga
    19681 ccacatcaac ttgcatttga aaaaaggaga gattgtagga atagctggac tgatgggagc
    19741 gggcagaact gaattcgcta tgagtctatt tggacattct tatggaagca agatatccgg
    19801 acgtatatta aaggaagggg cagagatatc agttaagacc gttccttcag ccattgcaaa
    19861 cggactcgct tatgtctcgg aggaccgaaa atctttagga ctgaatctgc tgatggatat
    19921 tcgggaaaac gtgacgctga gcagtttgga taaaataagt caaaaaggag tattagataa
    19981 agaaaaagaa gtaactgagg cagaaaattt ccggaaaaag atgcgtataa aaacaaactc
    20041 catccatcaa attgtcagta gtcttagtgg tgggaatcaa caaaaagtcg ttttatccaa
    20101 gtggctgatg acacaacccg atattctttt tttggatgag ccaacccgtg ggatagatgt
    20161 tggggcaaaa tatgagatct acacgattat cgaagaaatg gcagcaaatg gcaaagctgt
    20221 ctgtcttatc tcttctgaac ttcctgaaat attagggatg tgtgatcgaa tctatacgat
    20281 gaacgaagga cgtattacag gagaagtctc gcgagaagat gcagatcagg aagtcttgat
    20341 gaaattaatg acgaaagaag aggtgggtta agttggaaca ggcaaaaaat cttacggata
    20401 aaaaagagaa aaaactaaat ttgaaaagta gagcagctga tctttttggt cgctacagca
    20461 tggtgatcat tttagcggga ctacttattg cgtttcaatt gatgacggat ggtattttct
    20521 ggcgtccatt gaatataact aaccttgttc tgcaaaatag ccatatatta gtactagctg
    20581 cagggatgtt attagttgtg ctcttaggtc atgtggattt gtcggtaggg tcagtgatgg
    20641 cgtttgttgg tgcaattgcg ggcatgatga tggtcaatcg ccaaatgaac ccgtggatga
    20701 cagttatcgc atgtcttgca gtaggcgggc tgatcggcgc atggcaaggt ttttgggtag
    20761 cttatgcagg gataccggcg tttatcgtga ctttagcagg attgctgatg tttcgtggac
    20821 tgacacaagt tgttctagga ggccaatctc tagcaccttt tccagctgtt ttccaaaaaa
    20881 tgtctacggg ttatttacca gacttaagcg aaggaagcag ccttcacttc ttgacattga
    20941 ttttaggaat gatcatcacg atcgtgttag ttgttggtca atggcgaaca agaaatcgtc
    21001 ggaaaaatca tttatttgaa gtggaatctc caacagcttt cttggtaaag gcggtattta
    21061 cagcagtcgt tgtactagga ataacttata tctttgcctc ttatcaaggg tatccgatta
    21121 ttttgatcat tcttggtttg atcgttgcag catatgggtt tttgacaaat aaaacagtag
    21181 caggtcgaca gatttatgcg acgggcggaa atcgaaaagc ggctgaacta tcagggatca
    21241 aaacaaagcg aatcacgttc tgggtattcg tcaacatggg agtcatggca gctttagctg
    21301 gattagtttt agctgttcga ttgaatgcgg caacacctca agcaggtaca tcgatggaac
    21361 tggatgcaat ggcggcagta tattttggtg gtgcttcgac ttccggcggc attggaacaa
    21421 tcatgggtgc gattgtcggt ggcttggtca tgggtgtatt gaacaatggc atgtcgattt
    21481 taggcgtcgg ggtagactgg cagcaggcaa tcaaaggatt gatcttgctt ttagccgttg
    21541 tgctagactt gtataataag aaaaaacgaa caacgtagga gaacgccggt tattcagaaa
    21601 aaagactgtg acaagacatt tgtcacagtc tttttttcgc taaaagcgaa cattcattgt
    21661 ttcgtttgta tagactaaaa ataatatatg tttttttacc attcgtataa attctctttt
    21721 tgctcttgat ttttttgctt aattgagtta aattacatag ggcgctaaaa aaagccttga
    21781 ttaaattaat aatgttcgtg ttttgctgga tgtgaacatc aggaggataa tgtaatgaaa
    21841 gtatttgatt acgaagatat tcaattaatt cctaataaat gtatcgtaaa tagccgttct
    21901 gaatgtgata caacggtcac attaggtaaa catacattca aaatgccagt agtgcctgcc
    21961 aacatgcaga cgattattga tgaaacaatc gcagaattct tagcggaaaa tggttatttc
    22021 tatatcatgc atcggtttga tgaagcagct cgaattccgt ttatcaaaaa aatgaaaaag
    22081 cgtggattga ttacctcgat cagtgttggc gtcaagaaag aggaatattc ctttatagag
    22141 aagttggcag aagaatcgct aaatcctgat tatatcacta tcgacatcgc gcatggacat
    22201 gctaactcag ttattgatat gattcagcat attaaaaaat atttgcctga aacttttgtg
    22261 attgccggta atgttggtac accggaagcc gtccgtgaat tggaaaatgc gggcgcagat
    22321 gcaacaaaag tcgggattgg tccaggaaaa gtatgtatta caaaaatcaa gacaggcttt
    22381 gggactggag gctggcaact ggcagctctt agatggtgtg cgaaagcagc tcgcaagccg
    22441 atcatcgctg acggtgggat cagaacacat ggcgatattg caaaatctgt ccgttttggt
    22501 gcgacaatgg tcatgattgg ttcattgttt gccggtcacg aggaatctcc tggcgaaaca
    22561 aaagtcgaaa atggaatcgt gtataaagaa tactttggtt ctgcttcaga attccaaaaa
    22621 ggcgaaaaac gaaatgttga aggaaagaaa atctggatcc aacacaaagg atctttgaaa
    22681 gatacactag tggaaatgca acaagacctt caatcatcta tttcttatgc tggaggaaga
    22741 gatctggaag ccattcgaaa agttgactat gtcatcgtga aaaattcgat ttttaatgga
    22801 gacacaatct aaaaaataaa taaaatatta ggatgttgtg aaaaagttgt cctgcattaa
    22861 gtaataagaa cagccaaaca aaaatagttc tttatatgtt ttgtttggct ggaattatta
    22921 tcgcagatac aagcttttga acatcattat tcagaataag ggactgtgat aagatttttg
    22981 tcacagtccc ttatgttttg caaaaaaagt ttttcttttt tctcgtaaat tcggtaaaat
    23041 caaaagaaaa aatagacaat agaaaagaga agcttatgtg ggaaaaagta gagaaaaaac
    23101 aaggaccgat ttatcgtcaa attatggaac agattatgag agaaatcgaa aacggaagga
    23161 tcaatcctgg aagtcaactt gaatcagaac gaaaactagg gcaacttttt ggtgtgaatc
    23221 gttccaccgt tgtccatgca ttggaagagc ttagggagct tggggtacta acgagcaaac
    23281 gtggcagcgg atggtttgtt aatcagactg aatgggggaa attcgctgtt ccaagaatcg
    23341 attggcggca aatgatctct cctcgttatg agcagacgga ctggtatgag caaaaaataa
    23401 gagaagcaaa gcagatccaa aaaacatcgt ttcttgattt atacgctagc gagatgccag
    23461 atgagctttt gccgaatttt gagtttcctt cttattctct tgaagaaatc ttagcggaag
    23521 aaaaagaaat ggatctttta ggctatactc cgttacgtaa caaaatcaaa cggtatttgg
    23581 aaagtaaatt tgaattggca ttttccccca accagcttct tgtgactggt ggcggtcagc
    23641 aagcaatctt tttgatttta caaacgattc tttcagctgg agaggggata gctgtagagt
    23701 caccttcttt tttttaccga ttacctcttt tcaaagcttc aggaacgcgt ttatttggga
    23761 taccgatgga tgaagaaggg atcgacttag aaaaactgaa agaatcgatc cataaaaata
    23821 aattgaaagc agtgcttatc aacccgaatt ttcaaaaccc tacaggaaaa gtgatgagtc
    23881 aaaaacgacg agaaaagcta gtagaattgt gtcgacagta tcggcttcca attattgaag
    23941 acgatgtatt tacggatctt tcatttctaa accaagaaga agtccttcct atacgaacga
    24001 ttgatccgga taatgtgtta tacgtcggtt cgttatctag ggttttagga aaaacaacga
    24061 aaatcggttg ggtgatagga cctgagactt ttatctcgca actagctcaa gcgcaagaaa
    24121 tgatggagtt ttcgatgaat atccttacac aaatcacagt ggctaatgta tttaatgaag
    24181 caattgattt gaaaatgcaa gatgtaagaa aaaagttgaa agaaaaaagc cgactgataa
    24241 gcgcttgggg agattcacaa tcgtttttca agcttcattc aattttaggt ggttactatg
    24301 cctggattac ttgggatggg aaaaaactag accccgaact agcagaaaaa gtagtagata
    24361 tgggtcttgg aattgcacca agtctacttt ttgggcaaga tataaatgga atcagaatca
    24421 attacagtag gctggatgaa aaccaaatac cgttgtttta ttcaaagatg aaaattctag
    24481 aaaagtggtt aacagaataa tttcacaaag taaatagatt aaagaatata acagcttgga
    24541 tttaaatttc aagctgtttc ttttttttcg gaaaaagaat acgatataaa gaaactaaga
    24601 aaaaaccatt atttgtacgt ccgttcacga tgtgtaaata aaataaatgt ttgcaacaaa
    24661 actgtataac accaacgttt ttaaatagaa acagatggtt gtaagtttga ttagaaaata
    24721 gaaaaaatgc agatgaaata taaaacgctt tccttattgt gattttattc actaatgctg
    24781 ttaatatgcg gattggcata atttcctttc atcgtgaaaa tttgtgcttt tagcttgtat
    24841 ttctcataaa tccatgataa tttattaatg aagacggtta aatagtgata cagttttatt
    24901 cactgtttca atcgcagaat gaagagattt tcttatagat gagagaggaa tgtgtgacaa
    24961 atggcaaaca aaaaagcacc gatcgacttc caagcactgc tagatgaagt caatgcagat
    25021 ttcccagtat atcaagcact tgatcaagat ggaaacgtag ttaacaaaga tttagtacct
    25081 gatttatccg atgatgaact cgttgaatta atgactcata tggtatggtc tcgtgtatta
    25141 gaccaacgtt caacagcgct gaaccgccaa ggacgcctag gcttttttgc accaactgct
    25201 ggacaagagg caagtcagct ggctagtgct tacgcatttg ataaagaaga tgttcttctt
    25261 ccaggttatc gggatgtacc tcaactagta aaacatggtt tacctttatc tcaagctttt
    25321 ttatggtctc gaggacatgc tgcaggtaat tcttatccgg aagaattgaa agctctacca
    25381 ccacaaatta tcatcggtgc acaatatgta caagcagccg gtgttgcatt aggtttgaag
    25441 aaacgaaaca agaaaaatgt cgtcttcact tatactggtg atggcggttc atcacaaggt
    25501 gatttttacg aagcaatcaa tttcgctggc gcttaccatg caaatgccgt attttatatc
    25561 caaaacaacg ggtatgcgat ctctacacct cgtgaaaaac aaactgcagc aaaaacactt
    25621 gcacaaaaag ctgtcgcagc tgggattcct ggtattcaag tcgatggaat ggatccgctt
    25681 gcagtatata ctgtatctaa aatggcacgg gaatgggcga tcagtggaaa tggaccagtc
    25741 ctaatcgaaa cactgactta tcgttatgga ccacatacac tatctggtga cgatccaacc
    25801 cgctaccgta caaaagacac agatgacgat tggcagaaaa aagatccatt gatccgcttc
    25861 cgtaaatatt tgacggaaaa aggactttgg tctgaagaaa aagaagaaca agtcatcgaa
    25921 gcaactaaag aagaaatcaa agcagctatt gctgaagccg acaaagtacc aaaacaaaag
    25981 gtttcagatt tcttgaaaaa catgttcgaa gtatctccac aatcaatcaa agaacaaatt
    26041 gcaatttatg aagcgaagga gtcgaaataa tcatggcaca aaaaacaatg atccaagcaa
    26101 tcacagatgc cttagcactt gaactagaaa atgacgagaa tgtcgttgtt tttggtgaag
    26161 atgttgggaa aaacggagga gttttccggg caactgaagg attgcaggaa aagttcggag
    26221 aagaccgagt attcgatact cctttagctg aatctggaat cgcaggattg agtttcggtc
    26281 ttgcgttaga aggatttcgt ccagttcctg aaatccaatt ctttggtttt attttcgaag
    26341 caatggacga agtagtagct caaatggctc gtacaagata tcgaatgagc ggcactagaa
    26401 atctacccat cacaattcgt tcgccatttg gtggcggtgt tcatacacca gaattgcatt
    26461 cggataattt agaaggtttg atcgctcagt ctcctggtat tcgagtagtg attccttcca
    26521 acccttatga tgcaaaagga ctattgattt cttctattag aagtaacgat ccggttgttt
    26581 tcctagaaca catgaaacta tatcgctcat tccgagaaga agtgccggat gaagcttacg
    26641 aagttccttt ggataaagca gcagttactc gtgagggaac agatgtatct attatcacat
    26701 acggagcaat ggtgcgtgaa gcaatcaaag cagcagacaa tcttgcaaaa gaaaatatat
    26761 ctgttgaaat cattgatctt cgtacagttg cgccattaga tgtagaaacg atcattcaat
    26821 ccgtggaaaa aactggtcgc gtagttgttg tccaagaagc acaacgacaa gcgggcgttg
    26881 ccgcgcaagt agtttctgag atctcagaac gcgctatcct ttcattagaa gccccaatcg
    26941 gacgagtttc tgcacctgat acagtcttcc catttggaca agcagaaaat gtctggttgc
    27001 caaatgcaaa agatatcgaa gacaaagtaa aagaaatcgc ggaattttaa gaaaagaagg
    27061 gacgaaacaa agtcatggct tatcaattta aattgccgga tatcggtgaa ggaatcgcag
    27121 aaggtgaaat cgtcaaatgg ttcgtcaaac caggagatac gatcaatgaa gatgatacat
    27181 tactagaaat tcaaaatgac aaatcagtag aagaaatccc gtcacctgtt acaggaacgg
    27241 ttaaaaatgt gattgttcca gaaggtaccg tagcaaatgt tggggatgta ttagtagaaa
    27301 tcgatgcacc aggacacgaa gacaatgaag gggacagtgg tgtagccgca gaatcccaaa
    27361 ctccagctaa accggctgct gaacccactg ttgatacaga atcagctgga tcgtcaagcg
    27421 aaggagtctt ccaatttaag ttaccggata tcggtgaagg aatcgcagaa ggtgaaatcg
    27481 tcaaatggtt cgtcaaacca ggagatacga tcaatgaaga tgatacatta ctagaagttc
    27541 aaaatgacaa atcagtagaa gaaattccgt cacctgtcac agggacagtt aaaaacgtga
    27601 ttgttccaga aggcactgta gcaaatgttg gggatgtatt agtagaaatc gatgctcctg
    27661 gacacaatag tgcaccgtca actagtgcgc catcagctga agcaccaaaa gaaaaagtgg
    27721 aaacatcagg ttcagcaagt gtcgttgaag ctgccgatcc aaataaacgt gtattggcta
    27781 tgccatctgt tcgtcaattt gcccgtgaaa aagatgtaga tatctcacaa gtaactgcaa
    27841 ctggaaaagg tggcagagta acaaaagagg acatcgaaaa cttcttggca ggtggtccaa
    27901 gcagtgcacc agcgaaatca gaagcgccag aagcggcagc accgaaagaa gcagctcctg
    27961 cagcagaaag caaaccagct gcaccagcaa aaccattcaa gtccaactta ggagacttgg
    28021 aagaacgtgt agcaatgacg ccaacacgta aagctatcgc taaagcaatg gtcaacagta
    28081 agcatacagc tccacacgtg acattgcatg atgaagtaga agtgtctaaa ctatgggata
    28141 atcggaaacg atttaaagaa gttgctgctg caaatggtac gaaacttact ttcttgccat
    28201 atgtcgtgaa agctttaaca gcgactgtta agaaataccc agtattgaat gcatctatcg
    28261 acgatgcaaa ccaagaaatc gtttataaac attactacaa tatcggtatt gcaactgata
    28321 cggatcacgg actatatgta ccaaatgtga aagatgcaga ccgcaaagga atgtttgcta
    28381 ttgcagacga aatcaatgag aaagcaaaat tagctcatga cggcaaacta tctgcagaag
    28441 atatgcgcaa tggaacaatt acgatcagca atatcggatc tgtaggtggc ggttggttta
    28501 caccagtcat caactatcca gaagtagcta ttttaggtgt aggaacgatt gcacaacaac
    28561 caatcgtaaa tgctgaaggc gaaatcgttg tcggtcgtgt gatgaaactg tcattaagtt
    28621 tcgatcaccg tatcgtagat ggagcaactg cgcaacaagc aatgaataat atcaaacgtc
    28681 ttttagctga tccagagcta ttaatgatgg aaggatgata aacaaatggt agttggagat
    28741 tttgctgtag aattagatac agttgtaatt ggtgcgggac ctggtggcta tgtagctgcc
    28801 atccgcgctg ctgaaatggg acaaaaagta gcaatcattg aaagagagta tattggcggt
    28861 gtttgtttga atgtcggctg tattccttca aaagcactga tagctgcagg acaccattat
    28921 caagagtcac tagattctga aatgtttgga gtaactagtg aaaacgtcaa actagatttt
    28981 gcaaaaacac aagaatggaa agaaaacaaa gttgtccata cgttgacttc tggtgttggt
    29041 ttcctattaa aaaaacataa ggttgaaaca atcgaaggag aagcgttctt cgtagatgat
    29101 cacacactac gcgtcattca tccagattca gctcaaactt attcgttcaa taacgcaatc
    29161 atcgcaacag gttcccgtcc aatcgagatt ccaggattta aattcggagg acgtgtgtta
    29221 gattctactg gcggattagc attgaaagaa gtacctaaga aatttgtgat catcggtggc
    29281 ggtgtgattg gtgccgaatt gggcggagcg tacgcaaact taggagcaga agtaacgatt
    29341 cttgaaggct caccacaaat tttaccaact tacgaaaaag atctagtaaa attagtagaa
    29401 gatgatttca agaaaaaagg cgtaacagtc gtaacgaatg caatggcgaa agaagcagtc
    29461 gataacggtg acagcgtgac agtgaaatat gcagtggacg ggaaagaaga atccgttact
    29521 gctgattatg tgatggtcac tgtaggtcgt cgtccgaata cagatgatat gggcttagaa
    29581 caagcgggtg tcgaagtagg agaacgagga ttgatcacgg tggataaaca aggacgtacg
    29641 aacgtaccta acatctatgc catcggtgat atcgttccag gggcggcatt agcacacaaa
    29701 gccagttatg aagcaaaaat cgctgctgaa gcaatctctg gcaaaaaagt agctgtagat
    29761 tataaagcaa tgccggcagt agccttcact gatcctgaat tggcttctgt aggtatgacg
    29821 atcaaagaag caaaagatgc tggtatcgaa gcaaaagcat ataaattccc attttcaggg
    29881 aacggtcggg cattatcgct tggtaaaaca gaaggattca tccgtctagt tacgacgatc
    29941 gaagataacg tgcttattgg cgcgcaaatc ggtggagtcg gcgcaagtga tatggtctct
    30001 gaattagctt tagcaatcga atcaggaatg aatgctgaag atatcgcctt gacgattcat
    30061 cctcatccat ctcttggcga aatcgtcatg gatgcttcgg aattagcatt aggtttacca
    30121 atccatattt gatttgaata atacaggcga aacgcaactg aaaaatcagt tgcgttttgt
    30181 tttttagaat tatcaataat attattatgt aaacttaaaa atatgacaac ttcttggttt
    30241 attaccactt cctatgctac actgaagagg aatatgaaat aggagagttg aagatgcaag
    30301 aatatcagta tccacttgat ttagattgga caacagacga aatggtcatt gtaatgaaga
    30361 tgtgggaaga tctggaacgt gcgaatgaaa aagggataaa caatgaagct tttttgaaga
    30421 cttatcagca gtttaaaact gttgtcaaat ccattggtga ggaaaaacga ttgggacggg
    30481 aatttgaaaa agcttccggt tattctttat atcgtacagt gcagcaagca aaaaaaaatc
    30541 caaacaaaac tttgagaatg aacgggtgag ataaatggaa cagatgattg atcatatcaa
    30601 gtcctggctt gaagaagctg gtgagaaaat caaatcttct caacaaacat taacaatctc
    30661 gcaaaaatca aatcgaacgg atctagtaac aaatgtcgat cgaaacatcc agaattttct
    30721 aatcgaaaaa atccattcct ttgatccaga ggcgaaaata ttggcagaag aagaagggtg
    30781 taatcaatta tcggatctcg atggaagagt ctttatcatc gatcctattg atggcacctt
    30841 gaattttgtg attgaagggg aaaatttttg tattatgctc gctgtttatg aatccggcgt
    30901 tggaaagctt ggtttcattt atgacgtgat gagaaatgaa cttttttggg gtggaaaagg
    30961 aataggcgtg tataaaaatg atgagccatt gcctagacca gcaaataaat ccttatcgga
    31021 aggcttactt ggtgtgaata gttatctttt cggtcataat aaatatggaa tccgagaaat
    31081 tgcggaaagc agcatgggga cacgtatgta cggatgcgct ggtcttgaac tagtggcaat
    31141 cttgaaagga aaccacattg cttatatttc aaaccttagc ccttgggatt atgcagcggg
    31201 aaatgtctta ttggaagagt ttggtatggt aacaagcggt ctttttggta ctcctttgac
    31261 atttgaaaaa agggaatact atttagcagg gacttcttgt gcatatgcag aaatcgcaga
    31321 ccttttgaaa aaagaaggct gaaaaaaagg tagaaatcat tcacaaaaat gataaaaacg
    31381 agcaaaagat taattttctc gaaaaaatga aaacatttta ctatatttca tgaaaaaagt
    31441 ctgctataat tagcagtaga gtaaaatttg ccctgtcata aagaaccaat aaaaaaggag
    31501 ttagttacat tgaattatag aaacgatatc cgtaacgtcg caattattgc ccacgttgac
    31561 cacggaaaaa caacattagt tgatgaacta ttaaaacaat cagatacatt agacgcacat
    31621 acgcaattgc aagaacgtgc gatggactca aatgcgctag aaaaagaacg tggtatcacg
    31681 attttagcca aaaacacagc tgtagactat aaaggtattc gcgtaaacat catggatact
    31741 ccaggacacg cggacttcgg tggcgaagta gaacggatca tgaaaatggt tgacggcgtt
    31801 gtgttggtcg tagatgcgta tgaaggaaca atgcctcaaa cacgttttgt attgaaaaaa
    31861 gcactggaac aacatatcac accaatcgtt gtcgtaaaca agattgacaa accttctgct
    31921 cgtcctgagc atgtcgtaga tgaagttctt gaactattca tcgaattagg agcagatgat
    31981 gatcagttag actttcctgt gatttatgca tctgcactaa atggtacgtc aagcttgtca
    32041 gatgatccag cagatcaaga accaaccatg gcacctattt ttgatacaat tattgaaaaa
    32101 atccctgctc ctgtcgataa cagcgacgaa ccattacaat tccaagtttc attacttgac
    32161 tacaacgatt atgtaggtcg tatcggaatc ggacgtgtat ttagaggaac aatcaaagta
    32221 ggagaccaag tagcattaat taaattagac ggaacagtta agaaattccg tgtaacgaaa
    32281 ttatttggtt tctttggact aaaacgtcta gagattcaag aagcaaaagc tggagacttg
    32341 attgctgtct caggtatgga agatatcttt gtcggtgaaa cagttactcc tgtggatcat
    32401 caagatgcgt tacctatcct tcacattgac gaaccaacat tgcaaatgac tttcttagtt
    32461 aataattcac catttgccgg tcgagaaggt aaattcgtaa ccgctcgtaa gattgaagaa
    32521 cgtttgatgg cagaacttca aacagatgta tccttacgag ttgaaccaac aaactctcca
    32581 gatgcatgga cagtttctgg tcgtggagaa ttgcatttgt ctatcttgat tgaaaacatg
    32641 cgccgtgagg gatacgaatt gcaagtatct cgtccagaag ttattgaaaa agaaatcgac
    32701 ggtgtaagat gcgaaccgtt tgaacgcgtg caaatcgata caccagaaga atatatgggt
    32761 agtgttatcg agtccttgag cttacgtaaa ggtgaaatgc aagatatggt ccatacagga
    32821 aacggacaaa tccgtttgac attcttaaca cctgcccgcg gtttgattgg ttattcaact
    32881 gaattccttt caatgacacg tggttacgga atcatgaacc atacttttga ccaatatctg
    32941 ccaatgcttc ctggtcaaat tggcggacgt caccaagggg cgcttgtttc gatcgataca
    33001 ggaaaagcaa cgacttattc tatcatgagt atcgaagaac gcggtacagt cttcgtagaa
    33061 ccaggtacag aagtatatga aggaatgatt atcggggaaa acagccgtga taatgatttg
    33121 actgtcaaca tcacgaaagc aaaacaaatg acaaacgtac ggtctgctac taaagaccaa
    33181 acatctgtca tcaaaaaacc aaaacaattg acgttggaag aatctttgga attcttgaat
    33241 gatgatgaat attgcgaagt aacaccagaa agcattcgct tgagaaaaca aattttagag
    33301 aaaaatgctc gtgaaaaagc atctaaaaag aaaaaataat ctgtaaaagg atgaagagag
    33361 aaaccagctt gttataaaaa atagctggtt tctttttttg tccaactctt ttctgtggag
    33421 aagtcatctt tgtcaatgga cataaattca gaaaatacgc cgaaagacgt ttaaaagact
    33481 tttaaaaaac ttatttctag agagtttttg tttcataata agataatgtt tcacgaaagg
    33541 agatcaactt gtccttcgaa gaaaatcgaa gtaaaatggc aagagaaagg agcgggaaga
    33601 tgaacgactt attaaaacaa tactttggtt atgatgagtt tagacctggt caaaaagaaa
    33661 ttatccagaa agtcattgat caggaaaatg tattaggaat catgcctacc ggaagtggga
    33721 aatcaatctg ttaccagctt ccagctttac tattagatgg gcttactgtg gttgtgtccc
    33781 ctttaatctc tttgatgaag gatcaggtgg acgcggcgaa tcaattaggt atacccgcca
    33841 cttttatcaa tagctcgtta gatggttacg aaacagctag acgcttccaa gaaatcgata
    33901 gacagcagta ccgtttactt tatatcgctc ctgaacgttt tatcatgccg gattttatcc
    33961 aagcaatgaa gcgatggaac gtccgtatga tcgctattga cgaggcacat tgtatctctc
    34021 agtgggggca tgatttccgc ccaagttatt tacagatggc gaaccagcta gatcaactgc
    34081 caaatcgtcc tgtaattgtt gctttgacag ctacagcgac tgtgcaagta gctgctgaca
    34141 taaaaagact tttaaaaatt ccagaaaaca accatattca gactggtttt gaacgagaaa
    34201 atctacgatt tcaagtaatt aaagatcaaa aaaaagaaca atatctaatc gaatatttaa
    34261 agatcaacaa aaatcaatct ggtatcattt atgcagcaac gagaaaagaa gtagaccgtc
    34321 tctatcattt gcttaaaaaa ttcgattttt ccgtgggtag atatcatggt ggtctaaatg
    34381 agaatgagcg aacagaaatg caagaagcct ttttatacga tcgcttgcag ctgattgtag
    34441 cgacaaatgc atttggtatg ggtataaata aaagcaatgt ccgttttgtt atccattatc
    34501 aaattcctgg ctctctagaa gcatactatc aggaagctgg tcgggcggga agagatggtc
    34561 tttccagtga agccatcctg cttttctctc ctcaagatat ccaagtccaa aagtttttta
    34621 tccagcagtc ccaaagagaa gaagggcaaa agcaaaaaga atatgaaaaa ttaaaagcga
    34681 tgacagaata cgtgtatatt gagtcctgtc ttcagcaata catcctaaac tattttggcg
    34741 agacaagcag tccgtgcaac cgttgtggta actgtttaga tgacagagaa atagtagaag
    34801 taacgacaga agcacagatg gttctttctt gcttaaaacg tatgggtgag aactatggta
    34861 aacaaatgct gatgaaagtc ttagcaggat cgaaagaaca aaagctgcaa gctttaggtt
    34921 ttggacattt atctacctat ggactgatga aaaaccaatc gcaaaaagaa acgatgcagt
    34981 taattgaata tttgatatcg aatggctatc ttctgaccat caatggtgag tatcctgttt
    35041 tgaaagtaac agaaagaggg atacaagtat tgaaaggaca agaatctgtt tatcgaaagg
    35101 aacccaagaa agtccagcag ctatcggatg aagaaaccga tacactattt gaagtgctgc
    35161 gggaactgcg aacggatcta gcatcagaag caggcgttcc cccgtatgtc gtattttcag
    35221 attccacatt gaaagaaatg agccgaatac gtccatcaag cagattggag atgcttcaga
    35281 taaaaggagt tggccaaagc aagcttgata aatatggcga agccttttta tctcggataa
    35341 aaaatgcgga ctctaacgtt ctggataagt agaaagaacg aatacgtaaa aatgaataaa
    35401 aacaactggt taaatctgtt ttttttgctc cttatgaaaa tcaaatatgt tataattaag
    35461 gaattagaat gggaggtaag cacagatgga ttctttgtcg aaaaaacttg cgatccaagt
    35521 attaaatgat gaagcagaac ggatcaaacg attaatccga aatcaaaaca acagtctctg
    35581 tatctcacaa tgcaaagcct tcgaagaagt ggtagataca cagatgtacg gcttttcccg
    35641 gcaagtcggt tttgctgtcc gtataggagt aatcggaaag aacgagggcc agctgatgtt
    35701 aagtgagcta gaacgagaat tgaatcagtt atatagtgag gtttaccaag aaatttatga
    35761 taaaaaagaa gttggcaagg aggaatagac ttgcctaaga aagtgaaaaa aaggcatcta
    35821 ttagactata gcatcttgat tccctattta attttatgtg tattgggatt gatcatggta
    35881 tatagttcta cgtcgtatct attattagaa aacggacaaa atccgtccgc atcagtcatt
    35941 aaccagagta ttttttgggt gcttagcttg attgctatcg cactacttta taaaatgaaa
    36001 acagatgttt tgaaaaacca gcgtttgatc atggcagcca tcgcagtttt gacgattttg
    36061 ctgctgattg ttgtgttttt tggtaaggag atcaatgggg cgaaaggttg gctgcaaatt
    36121 gcaggattct cgatccaacc tgccgaatac ctaaaaatca tttctatctg gtacctgtct
    36181 ctgactttat ctaaaagaca aaacagtgta caaaaagatt ttcttgggac tgtgaaacgt
    36241 ccacttgcaa tggttatcgg acttactgca ttagtcgcta ttcttcccga ttttggtaat
    36301 gcggcagtta tctttttgat tattttggtt ctgcttttag ccagcggagt caattatgtg
    36361 tatactttga ttgtaggagt aggaggcttt tgtctaagta cttttacgat ctggctgatc
    36421 aatatcacaa acggaaagat cttacccgga cgtttacagt atatctataa tcgatttgct
    36481 atttaccaaa atcctttctc agacgaatta aataaagggc atcaactagt caacggctac
    36541 tatgccatgt tcaacggagg actatttggc aggggattag gtaacagtat ccagaaaaaa
    36601 ggatttttac aagaagcaca aaccgatttt atttatgcaa tcgtagtgga agaactggga
    36661 gtgattatgg ggattttgat attagcgttg ctattcttta tgattgtgcg cattgtcctt
    36721 gtaggtatcc gttcaaaaga tccgttcaat tcgcttttgt gcattgggat cggcgcaatg
    36781 tttttgatcc aagtatttgt caatcttgga ggaattactg gcgttattcc tttgacaggg
    36841 atcacgttcc catttttaag tcaaggaggc tccagtttgc tgatgctttc gatttgtgtc
    36901 ggctttgtcc tcaacatcag tgcggatgaa aaacgcaaga gcttaggttt ataataaaaa
    36961 aagatgtagg agtggttcga tgaaaaaagt tttagttgct aacaggggtg aaatcgctgt
    37021 acgtgtattt cgtgcttgta cagaattagg aattaaaact gttggaatct atgcagaaga
    37081 agatgaatat tccgtacacc gtttcaaagc ggatgaagca tatttagtag gacaaggaaa
    37141 aaaaccgatt gatgcgtatc tagatattga agggatcatt tctatcgcga aagaatgcgg
    37201 agctgacgct attcatccgg gttacggtct tttgtcggaa aacttgaatt ttgcgcaacg
    37261 gtgcgaagaa gaaggcatca tttttgttgg acccaaactg catcatttag atatttttgg
    37321 agataaaatc aaagcaaaag ctgcagctat cgaagcgggg attgcttcga ttccaggcac
    37381 agatggacca atcgcttcta tagacgatgc cttggagttt gcgaaacaat acggttatcc
    37441 gattatgatc aaggcagctc taggcggcgg cggacgcggc atgcgtgtag ctcacgatga
    37501 aaagagtgca agagagggct acgaacgtgc gaaaagcgaa gcaaaagcag cctttggtag
    37561 tgatgaagtg tatgttgaaa aatacatcgc taatcctaag catatcgaag tacagattct
    37621 cggggatacg catggaaacg tcatccatct ttttgaaaga gattgttctg ttcagcgccg
    37681 tcaccaaaaa gtagtagaag tagcgccttg tgtctctatg aatgaacagc aacgtcaaaa
    37741 aatctgccag gcagctgtcc aattgatgaa atatgtcggg tatgtcaacg caggtacagt
    37801 agaatttctt gttgaaggag atgattttta ctttatcgaa gtcaaccctc gcgtccaagt
    37861 ggaacatacg atcacagaaa tgattacaga tattgatatc gtaacaacgc aactgttgat
    37921 tgctcaaggt ttggatctgc ataaagaaat cggattgcct cagcaagaag gaattaaatt
    37981 aaatggttca gccattcaat gccgaatcac tacagaagac ccgttgaata actttttacc
    38041 agacacaggg aaaatcgata cgtatcgttc acctggtggt tttggcgtac gtctggatgt
    38101 cggaaatgct tacgcgggct atgtagtgac accgtatttt gattcattgc tagtaaaagt
    38161 atgtacgcat ggtgctacct ttgaaacagc cattcagaaa atggaacgtt gcttgaaaga
    38221 atttagaatc cgtggagtga aaacaaacat tccatttatg ctcaatgtca tcacacatcc
    38281 tgaattccaa tcaggaaatg caaaaacaac cttcattgat agtacagcaa cattatttga
    38341 atttcctcga ctacgagacc gcggaaacaa aacgatgaaa tatatcggtg aaattactgt
    38401 gaatggtttt ccaggtatcg aaagtggcga aaaaccattt tatgaagaac cacgtatgcc
    38461 taaagatcta atcacaagaa ctgattacgt aacggctaag aatgtattag atgcgaaggg
    38521 tgccgatgct ttggtcgaat ggatcaaagg acaggaaaat ttgttgttga cggatacgac
    38581 atttagagac gcccatcaaa gtctgctagc aactcgtgtt cggacaaagg atttcaagca
    38641 gattgcacgt ttgactggag aaggtcttcc tgaactcttt tcaagtgaga tgtggggggg
    38701 agcaacattt gatgtggctt accgcttttt gaatgaagat ccttggcaac ggttgagaaa
    38761 aattcgttct ttaatgccta atacgttgct gcaaatgctt ttcagaggtt caaatgccgt
    38821 tgggtattct aattatcctg acaatgtatt ggtggaattt gtcaaagaag cagcagcgca
    38881 aggaatcgat gtatttcgaa tctttgatag tttgaactgg actccacaaa tggaaaaaag
    38941 tatccaagct gtacgggata ctggtaaaat cgcagaggca gctatttgct atactggcga
    39001 tatcaacgat ccaagtcggg caaaatacaa tgttcagtat tataaagaca tggcaaagga
    39061 actagaacaa ctgggtgcgc atatcattgc aatcaaagat atggctgggc tgcttaaacc
    39121 gcaggcagct tatcgtttga ttagtgaatt gaaagaaacg acagatctgc caatccatct
    39181 gcatactcat gataccagcg ggaatggaat catcacttat tctgctgctt caaaagcagg
    39241 agtagacatc gttgacgtgg caatgagtgc aatgagtggg aatactagtc agcctagcat
    39301 gagcagcttg tattatgcac tggtcaatgg tccacggctg ccagaaatca cgattgaaaa
    39361 tgcacagaaa ttaaaccatt attgggaaga tgtgcgcatg tattacaaac cgtttgaaaa
    39421 cggcttgaat gctcctgaaa cagaagtata tatgcatgaa atgccaggag gacagtattc
    39481 gaatctacag caacaggcaa aagctgtcgg attaggtcat cgctgggatg aaatcaaaca
    39541 aatgtatcat actgtcaact tgatgtttgg cgatatcgtt aaagtaactc catcatctaa
    39601 agtagttgga gatatggcac tatttatggt ccaaaatgat cttacagaag aagatattta
    39661 tgaaaaaggc gaaacactaa gtttccctga atctgttgtg actttcttcc aaggagaact
    39721 agggcaaccc gttggcggat ttcctaaaaa attgcaaaag attattctta aaggcagacc
    39781 tgcattgaat gaacgtccag gtcttttggc tgaatcagtc gacttcaacg aagtaaaaaa
    39841 agaattggca gaaaaaattg gctatgaacc aaaacaagac gaagtattaa gctatttgat
    39901 gtatccgcaa gtctttttgg actatcaaaa agcatataat caatttggcg atgttacatt
    39961 actggacaca ccaacatttt tccaaggtat acgtttgggt gaaacaatca atgtacaaat
    40021 cgaacgcgga aaaacgctta tcattcgttt agatgaaatc ggagaacccg atatcgaagg
    40081 gaatcgcgta ctgttcttca atttgaatgg acaaagacgt gaaatcacta tcaaagataa
    40141 ttcgatcatc agtacggtcc aaacccgatt gaaagctgaa ccgacaaacc gagaacaaat
    40201 tggagcaacg atgtctggtt ctgttttgga tgtattagtg aaaaaaggcg ataacgttaa
    40261 aaaaggtgat acgttgatga ttactgaagc gatgaaaatg gaaacggcca tcgaagcacg
    40321 ttttgacgga gaagtcgcac atgtatatgt gtcttcagga gacactattt cgtctggtga
    40381 ccttttgatt gaagtgactg agaaataata agcgaaaggt ggggaaacat gaaaagactg
    40441 ggtttgtttt ggctgattct gctattagtt attgtggcat tctacatgga accagtcttg
    40501 tttccgccaa aacttgaagt gaaggaaaac tctgatcaac aaatatcaaa ccaaatgaca
    40561 acgtggaaat accaggagct taaagcaact ggtttttcta cttatatcgg aaaaccgatc
    40621 gatcagctgg aaaaggattt tggccaagcc tatgatcgat taactagcgg atttggtttt
    40681 gaaacaagat attatgaaaa taaagctagt catctcacat ttgaagcgaa catccaagac
    40741 agcagagtca caactgttaa agtactgcaa acagggcctg ctgatatcgc gccgttttat
    40801 ttagggatga cgatgcaaga tcttacgaaa atcactacta tctatactaa tttcactttt
    40861 gagtaccatg atacagaagt tggaatcgaa ctaatggaag aggatatgaa ttatcgtccg
    40921 ctaattgctt ttgataacga cacttttgct attttatttt ttgatcaaac aacaaacggc
    40981 ctattttcag ttgtgtattt agataaagat agtcttttga aactattgcc atatcaagtg
    41041 tttggtgaag gccttccaca ttatcaagca gataaatcgg ctgattggga atcaatcaat
    41101 cgcgttaaag aacgaaaaag tacgacttta ttaaatatgc ttagaagaga agacgaactt
    41161 cccatataca atcaaacact tgcctttcaa aaccacagtc agctgctact acatgatttt
    41221 ttgacaaggc ctgaggaaat attgtcagag gaacgtatgg ctgattggca aaaatcgcta
    41281 ggcagtgctc aaacgacagt gaaattctca ctgacgaatg atgaattgga gaagttaatc
    41341 agtaaacaaa aattagaaaa tactagtggg ctatttattc atccggcgat tgacgctact
    41401 ttttcgtttt tatattggta cagcgatcct tacaaccatg accgtttcat ggacccggga
    41461 acggattctg taggcattgc tttttctaaa gaaaatatgt tagtcttatt gcaagaagaa
    41521 aacggagaaa gtagtgatag cagtgatcgt taacgaagag ctgtttcaac tggaggatca
    41581 atgcctccaa gtcgctgaaa tgataaaaaa gagtaaaacg atggcttctt atcttgaaca
    41641 taaacagtca atggaagaat ctgcaaaagt ttgccagttg aaagaagcgt tcttgtctaa
    41701 gaaagaagca tttgaaaatg tagcggcata tggtgagtat gcacctgatt atcgtgaaaa
    41761 aaaacgagct gttcgaaaag caaaaagaca gcttgatatg gatgagcatg ttgcagcttt
    41821 ccgatatagt gaaacacagc tgcaaagtct gttggatcaa attgtccaag tacttgccga
    41881 agaagtttct aatgaaatca aagtagatgc aggtagcccg ttttttgaaa cgaaaaaaca
    41941 ctttggatgc ggaggtaatt gtcatgcaat tagacaatag tgaatttgaa ataacaaaac
    42001 gacgcgggct gatcgtatgg gtctacagct taaaacaatt aaagaatctg aaaaagttcg
    42061 gcttgttgca ttatgtctca agaagaatga aatatgtcgt gctttactta aatgaagaga
    42121 cattggaaga aacagaagaa cgaatccgta aattacattt tgttcgaaac gtagaacgtt
    42181 cttaccgacc ggatatcgaa atgaattttg ccgaaaaaat aggaaacaaa acagtattaa
    42241 aagacgaagg tttcgaaatt gaagagctaa atacagaaat ccgattagct gaacatcttt
    42301 aaaaatctct tatgagcagt ttttaaataa aaaataactt ggataacgat cgatttttga
    42361 tcattgtcca agtatttttt tggtatacta gtaccagtac catttctaag gagtgagtag
    42421 atgcggataa tatcaggaaa ttatggcggg cgccgtttaa aaagtttagc aggggcgaac
    42481 accagaccaa ctatagataa agtaaaagaa tccatattca atatgattgg accatatttt
    42541 gatggagaga cagtattgga tctgtttgca ggaagcggtg gtttagctat cgaagcagta
    42601 tctagaggct gcagtcatgc ggtatgcgta gacaaaaatt accaagcgct aaaaatcatc
    42661 aaagaaaaca tcgaaattac gaaagaacca gagaaattta ccacaataaa acttgatgcg
    42721 gacaaagcaa tcgaacggct agcagcggag aaacaacaat tcgactactt gtttcttgat
    42781 ccgccatatg caaaacaaaa aataatcgat caaatccata aaatggaaga actgggactt
    42841 ttttctaaaa ccgcagttat cgtatgcgaa acggataaaa cagtcgaatt gccagaaact
    42901 gtggctggat tccatcaaat aagaaaacag atttatggca taacggccgt aactatctat
    42961 agaaaagagg gaaatcaatg aaacgtgcat tattcccagg aagttttgat ccttttacaa
    43021 aaggtcactt agatacggtg gaacgtgcgg ctaaattatt cgatgaggta gtaattggtg
    43081 tttttatcaa tacttcaaag aaaagtttgt ttcccccaga agaaagaatg acattgatca
    43141 caaaagctgt ttctcatctt cctaatgtaa aagtcatgca tcaagaaaat caattgacgg
    43201 tagaaacagc aaaagagatt ggtgcagatg cgttgatcag aggcatacga agtatcaaag
    43261 actttgaata tgaacgagag attgctcaga tgaatcatca tctatatgat gaattggaaa
    43321 ctgtcttctt attagcaaaa ccagaatata gtcacgtgag ttccagtatt ttaaaagaag
    43381 tactgcactt tggcggagat gtgagcagct acttaccgcc agtgatcaac gaagctttag
    43441 ctggaaagcg tgaatcaaat gaaagctaaa caatggttga aaacagtact gattatcata
    43501 tttggaatgt tgatcattgg atttgtagta gttcccttgc cgtattatgt agaatcaccg
    43561 ggagccacga tcgatctgaa agaactaatt acggtcaatg ataaggaaga taaaggttct
    43621 gggtctttct ctcttacctc tgttggtatc aggcaagcta caggtttctt agctttaaaa
    43681 gctaaaatga ccccttttga agaattgatc agtgaagaag aattgactgg tggggcgaca
    43741 aatgaagaat ataatcaaat gcaacagtat tatatggatt cttcccaaaa tgcagcaatc
    43801 gaacaagcgt taaaacttgc agacaagcct tataaaatgg aattcaaagg cgtatatgtc
    43861 atgagtgtag agaaaacatc gaatttcttt ggcaagcttt cagttggaga tacagtaaca
    43921 aaagtagacg ggaaaagctt tcaaagtaca gaagcattta tggattatgt aaaaagccaa
    43981 aaagttggac aaacaatcga aatcagttat ttgcataatg gtgaagaaaa aacagcttct
    44041 ggtgatctga tcgagttacc aacagataaa aaggcaggaa taggtatcac gcttactgac
    44101 cataccgaga tcgaatcaga tacagatgtt cgtattaatt ctggctctat cggtgggcca
    44161 tcagcgggat tgatgttcac tttagaaatc tatgaacaag tcactggtaa aaacttgcga
    44221 catggtaagc aaattgcagg aactggaacg atcaacagtg aaggagaagt tggccgaatc
    44281 ggaggaatcg ataaaaaagt ggcaagtgcg gataaagctg gcgtagagat tttctttgca
    44341 ccagatgata cgatttcaga cgatgttaag aaagaatatc ctgaaataaa aaccaactac
    44401 gaagaagcaa aagctgccgc taaaaaattg aattcctcaa tgaaaatcgt accagtaaaa
    44461 acagtacaag aagcgcttga ctatctagag aaaatgaaat gaaactaaat aattaaagag
    44521 gggttgtgac acgagttctg tcacaacccc tctttttcgt caaaaatctt cacgataaaa
    44581 ataaaaacaa aagaaaaaat tgacgtaatt agatgttgaa gggacgattt tgccagagct
    44641 cactgtttct tcaattttaa atgaaaggag taatgaatga tttttactgg tcattctttg
    44701 aaaaaagaac aatgatagaa aaaatcagca agaaaatact agcttcaccc aaattgatca
    44761 tttggatgtc tatgatagtt cttatctttt gtatcgcaag tggtttttta ttatcaaatt
    44821 tgctatttta tagagaaaca gaagagactg ctgcaaaaga aatactttct tcgtttagtt
    44881 ccagtggaga agatccatca gctgaaggaa acgatgtaga agaggatgct cgtccaaagg
    44941 tcatgtacac cgatatcaaa ggaagtgtga aggagcctgg tatctattcc ttttcttcag
    45001 aagaacgggt ctatgatgta ctaaagcgtg caggtggatt attagaagaa gcggatagcg
    45061 atcgaatcaa cttttctgcg aagatagaag atcagcaagt cctatatatc cctgctgtag
    45121 gtgaagagct gccagaacat cttgatcagt tggcatcctc ggaaggaaaa cagcctacag
    45181 cagattccga gcctcccaaa ataaacatca atacagccac tccatcagaa cttcagcaga
    45241 ttcctgggat cggatcagtc aaagcgcaag aaatcattcg atttagagaa gaaaacggat
    45301 ccttccagaa agtggaagac ctgcaggaga tttctggtat cggtgaaaag acagttgaaa
    45361 aactaaaaaa cttcgttaca attaaataga atgattcaaa atcgactggc acgagcaata
    45421 aatggacgct aatggatact aagaataatc caattctaag agcttggcag gacgaggagg
    45481 tagtattttt ggataggaaa tagcttgatt gttcgcaaag aaacattaaa aaaaccaatg
    45541 ctgaatgtct atctccaaaa taaaatatct gggattcata ttatgaatac ggctgtcagc
    45601 ggcaataaca gtcaagctct acgagaacgc tttgccaaag atgtacttag ctatacagca
    45661 gataaagtat ttattctcat tgggacgaat gatctagcag agcataaaca actttctaaa
    45721 gaaacgtacc aaaaaatctg cagtggatga tagttcagct gaaaaaagac tatagttctg
    45781 atcatgcttt ctttatcacg ccaccagcag tcgatgagac aaaacaaaaa tatcggaata
    45841 acaagttgat caaagaatat atctctgttc tcacagaagt agcaaaacgc catgattgtg
    45901 aagtatcgat ttttatacgg aatttcttga acaaatggat tcgaagaatt tttataatgg
    45961 gctaatggat gacggacttc atttcggcag tgaagggtag gatcttttgt cagacaggat
    46021 gattcgttat atttaaagaa tattttgttt aagcaatgag tactttttaa tcaacttgaa
    46081 aaaacggagg aatataaaac atgttagaaa aatcttcaaa agcatgcgat acgaataaag
    46141 aaaaagttac tgataagtca cataatgaac gcattccttg ggaccagtac tttatggctc
    46201 aggcagtact gttatcttta cgtagtacgt gtacacgttt agaagtagga gcgacactcg
    46261 ttcgagaaaa aaggatcatt gctggcggct ataatggagc agtcagtgga gatgtccact
    46321 gcatagatga agggtgttac gtcgtggatg gacactgctt gcgaacgatc catgcagaga
    46381 tgaatgcact cctacagtgt gcaaaacttg gtatcccgac ggaaggttca gaaatctatg
    46441 tcacacattt tccatgtttg gcctgtacaa aggctttgtt gcaagctggt gtgaaaggga
    46501 tcaattatct ccatgactat cgaaatgatc cttatgccca agcgttgatc gaacaattag
    46561 gtgtatctgt acaccaagtt gctttagact cacagcattt ttcaaaactt caaaatgaat
    46621 taacaggaaa acatgtctag cgttttagaa aggcatgttc gaaatcggtt gatttttccg
    46681 gcaattacgt cgatgattat ctgtttgttg tattacactg acaattgggg ttatcgtttg
    46741 cttcttgccg gaattttttt atggttttcg ctaaaagaag gacgttttat cgcgtacttg
    46801 tgtattttgt gtagcataac ggtatccatt tcttataggc tacaagagta ttcattttta
    46861 gaggaaaaaa caacatctgc gcaaattact gtatttacag atacgattcg tgcaaatggt
    46921 aattttttga caatggaagg aaggttatca gagaataaac aaaagctcca gctttcttat
    46981 caagtaaaca cagaagcaga attgaaaaca tggcttaact tccgaggaaa gggaatagaa
    47041 ttgactgctg aaggcgagtt catctctcca gcaaaaaagc ggaatcttta taattttgac
    47101 caagaagctt acttcaggaa ccatagaatt tctggacggt tccaaattca aaagattgaa
    47161 cagatcacca tgcaaaatca ctggcgaaac tggcttcaga gaaaaagagg atcttttatc
    47221 tcctttgtcc aatctgaatt tccgcaaaaa acaagtgtat atattaattc tttattatgg
    47281 ggctataaag atgcaggatt cagagaacca gaagaaattt ttagggagag tggtttgctg
    47341 catctgttca gtctatcggg tctacacatc caattttatt tagggtggct gtattattta
    47401 tttcgacgta tcggatggcc actacacgtc agtattattc cgttgagtct actaactatc
    47461 tgttatgttt ctattgcagg aagttctatt tctgttttgc gtgctagttt gctgttttta
    47521 ttgcgattgc tagtcaaatt gctgaataag cattattcca ccatggactt atttgcagtt
    47581 gtcttttggc ttcttttact atttgatcct cgatttcttt ttcatgcggg tggacagctt
    47641 agttttttca tgagtttttt gtttatcctg atttcttttg atggagattg gttgaaaaaa
    47701 acggcctctc acgtcctgtt tactatgatc acgatgccgt tgattgtatg gcatttttat
    47761 gagtggccta ttttaggtag tctttttaca ttactgattg caccggtatt caagtttctt
    47821 tttcttcctg gtgtgttatt tctcacactc ttcaatcgct ggctgccgga aactttactt
    47881 ttgctgatag aagagggatt aatcttattt gagcgaataa tcgatttttc ttcagggtcg
    47941 tctctcatta taggacgttt gccccttgtg ctatctggga tgtatatttt cggattactg
    48001 atttgtatgg ataggttgaa agaacaacct gtgaaacact tgttttatgt tggtcttttt
    48061 tcttttattt ttttgacttt gccattttta cgcttttctt caacgatcgc ttttatcgat
    48121 gtcggtcaag gagacagtat ctttttgcaa agtccttttc gaaaagaaac gattttgatc
    48181 gatactggag gtcagttaca ctttgaaaga gaaaagtggg cgcaaggtat gatacgtcct
    48241 cctgctgaaa gaaatatcgt tccatatcta aaaggtcagg ggataagtgt aatcgataaa
    48301 ctcatcttga cacacgacga tacggatcat gtgggagaat tacccacgtt atctcagcat
    48361 tttactatca aaaaaattta tatcggttgg ggcgctggac gaaatgagcc attacaaagg
    48421 catttatctg aagctcaaaa aaatggtact gagatcattg aaatcaaaca tggggacagg
    48481 atcagcggtt attatgatct ttatgtattg actccttttg aaaagggaga gggaagaaat
    48541 gaagattcga ttggtctatg gatggaatat aacaataggc gctttttatt tttaggagat
    48601 ttgaatcaag caatggaaaa acaattgctt ttgatttatc ctaatctgaa agccgatgta
    48661 gtcaaattag gtcatcacgg tagccgtacg tcaagtaaca cagattttct ttcccatatt
    48721 gaagcaaaac atgggattat atcctgcggc gtaaataatc gttatggtca cccgcacagc
    48781 gaagtattgg agacactaga agaaaatcag ataaaaacat ggcgaacgga tcaacaagga
    48841 atgatcacct atcgctggca tccagttttt catcctcatg ggctagtgga aacaatgata
    48901 gactagtaat tctcatctgc tcatggtagg ataatttttg aaagtggtga gtaatttgag
    48961 tatacaagca gagttacaaa aaatacgtaa agaaaaactt gcatcttgtt acttgatttt
    49021 aggaacagaa aagtttctcc aagatcaagt tcggacagaa attgtcaaaa aaatccaatt
    49081 agagggcgaa gatgacttaa attttttaag ttttgacatg gaaaatgcaa gtttagatga
    49141 ggtagtagct gaagcagaga cgttgccgtt tttcggagat caacggttag tattcgtaga
    49201 gaatccttat tttttaacag gtgaaaaagt aaacaatgga atcgaacaaa atactgattt
    49261 attgacggct tatttgaaag aaccattaga atcgactgta ttagtattct ttgcgccata
    49321 tgaaaagttg gatgaacgga aaaaagtaac gaaacaattg aaaaaaacag caattatcat
    49381 caatgtccaa cagttgaatg aaaaagaagt acgtcaatat ctgatgaata ccttagagaa
    49441 ttcctcaatc aaaatggaac gaagtgcaat tgatttattc ttgaggttga ccgacttaga
    49501 tttatccaaa atgatgagag agttacagaa attggtcctt tatggacaaa atcaacagca
    49561 aatcacggta agagaagtag aagaattagt tcctaaaacg ttagaacata atatttttga
    49621 catgacccaa tatattttaa aagggaagac ggaacaagct ttgcgtttat acgaagattt
    49681 agttatgcaa ggggaagaaa caatcaaaat taatgcgatt ttgctttctc aattacgttt
    49741 atttttacag acaaaatttc ttgtaaagat tggttatcag caggcaaata ttgctgaaac
    49801 attgaaaatc catccatatc gtgtaaaact tgccatgcag gaagtaaaaa aattcgatga
    49861 acatttgctg gtacagttgt ttgatcaatt agttgagatg gattatcaaa tcaaaacggg
    49921 acaaatcgaa aaagaattaa gttttcaact attcgttttg cgcacagggc agttgttaag
    49981 aaatcgataa gaagaaagac gagggcaaag catgcgggaa ggtcgtttgt ttttgtcctt
    50041 tttttgacaa ataaaaaaat cggccgaagc cgattctttt atttagcgat ttttttgctt
    50101 aaacgagatt tgtcgcggtt agctttgttt ttgtggatta gaccttttga ttcagccata
    50161 tcaatagctt taacagcttc tttatataac gcatctacgt tgtcagcacc tgaagctaca
    50221 gcatcttcaa attttttgat tgcagtacgc atagcgcttg tttgagatga atttttcaca
    50281 ttagcgtttt cgcttgtacg aacacgtttg attgcagatt caatgtttgg cattagtttc
    50341 acctccgata acttaagttc ttacgcgtgt taccgcataa ttcaacattc attattatac
    50401 ctaaaggcag cgtgctttgc aatatgaaca tgacaagttt ttttccttaa cagtctttta
    50461 ttaggatttt ttttcagtaa aaacggatag tacaagctga aaaaaatcaa aaagacgcta
    50521 ttgtgggagg caatagcgtc aaaccaaact agatttttat ttatctattt aaacaaagtt
    50581 tgaatgggaa tgtttggtac taagttatta tataacaaag agaaccgttt gcaaagtttt
    50641 ttttgaaaag ggatacgatt ccctgttgtt ctttgttttt ctattaagaa actgcagtta
    50701 ttttataacc agtatccttt gatcagtaaa ggctattctg gtgtaccgat tattttaatg
    50761 tattcatcga tattttttat caacattttt tttatgacca actggttccg acggttccat
    50821 ttgccactta cttccagcac atatttattt aggggcaaac gtaagatata ttttgccaaa
    50881 tagcgatctt taagcaggcc attgatcaca cggtcctcaa gctgcaagtt gatcccagtc
    50941 agcatcattg gctgttcttg taaaacgtac gtatcaatta aaagaccttc aaaagtttcc
    51001 acaagcgtct tcctttcttt tcaaagtaac ctgaaaacag gcactaattc ttccattttg
    51061 atattcgttg aagtggatgc gatcattctt tcttttgtcc actgtaatct agtataacaa
    51121 aaaagagcag aagagccaaa aagaaaaaag agcatcgaat tctataggaa gtctcattgg
    51181 cacggcctaa attattacaa aacattgatt aaaaaaagcc cctgcatcgc aggagcatcc
    51241 cctatatcgt tttaataggt caatcaaaaa gcagtatcaa acacattttg tatcaaatca
    51301 ggaaataata gataagctaa gcccagaatc aaaagtaccg ctagaagaaa ccagccgatt
    51361 gttcctatga aatactttaa tttttcgaac attgtatcac ctcgttgtca gtatatcacg
    51421 gatcagtaaa ttgggggtgt taagaatctc ctaaaataga caaaatttgt tctattttcc
    51481 taaagcaaat acttgtaaga tacagtaagt gataccagaa gcaatgaccg gtccggcggc
    51541 gattcctctt aagaagacaa cacctaaaat cgtgccgaaa acaagtgcaa cagtgatttg
    51601 tggatcagaa gcaatcaatc ctacaccctt ggaagataag acagccacta gcccaccgca
    51661 aaaaacagct acccagccaa taggggattt aaaggcttct aatagatcac gtagaccgat
    51721 ttctcctgtt gcgataggga ttagaatagc cgctgtgatg atcgtcaccc caatattgat
    51781 tcctttgcct tgaagccaag ggaaaaagga acgggaggct ggtatgagct tcataatcaa
    51841 aacaaaagct gccgctatca tcagactttg atttttagct aaaattgcta caagtaaaat
    51901 aattcctaaa aaaagccaac tttccatgaa atatattcct cactttcttc gaaacaatca
    51961 taacatgttt ttagaaagtt acaaaagtag gaattcatcc ttcaagacct attcataagc
    52021 tgtttttttc ataaaataaa caaaatcata aaaaaacact ttgcttattt gacagaagac
    52081 agccgcgctt tgtatagtgg acaagactta catttttttg aagaatcaga aagatcaggt
    52141 aaaggtctta gtaaacgcaa gccattcaga ataaccaata ttgtgcttcc ttcgtgtcct
    52201 acaactccaa gaggtagatt taaaaattgc aggaagttag aagcaatcaa caagagaata
    52261 acaagaatcg aaaaaataat attttgtttg atgatccgcc gcatttttaa agatagccga
    52321 tgactgtaga caaatttatt taagtcgctt ttcattaaga cgatgtctgc gatatccata
    52381 gcggcatctg tcccttgccc catagcgaca cctaaagaag cggctgctaa agcaggcgca
    52441 tcattgactc catcaccaac catcgcattt acttggtaaa gttcattctc ttttttgact
    52501 agttgtgttt tttcttccgg agtacagctt gcatagtaat gttcaatccc tacttgttca
    52561 gcaatggcct ttgctgtgcc agaatggtcg ccggttatca tacttgtatg gatgttttgg
    52621 gatttaaagt aggaaattac ttgttgggta tttgcttttg gaatatctag cagacctaaa
    52681 acagcaatta actgctgatc tttagaaaga taaatcacgg ttttgccttg attttccaaa
    52741 cgttcgatca tttccgcaat ctctttgctg attatcatcg tctcaggatc atatgcgtgc
    52801 ttgccaactt tccattgcgc acctaaatag gtagtttgta atccaaaacc agtaatttct
    52861 tctattggta agtattggat ttcttcaggt attgtgcaat cgaaatattg caaaatcgca
    52921 tgagcaagtg gatgcgtcgt tttttgttcc atagcaacaa gaagttgttg tgccaaatcc
    52981 ttttcatcaa agaaaaagct gtcagttaca actggttttc ctttagtgag cgttcctgtt
    53041 ttatcaaagg caatcgcttt taattcagaa agcttttcta aatggatacc gcctttgatt
    53101 aaaatcccat tttttgcaga atttgaaaga gcagctagag aagctggtgt agcagatgcg
    53161 acaagcgcac aaggagaagc cacaactaat aataccattc ctcgataaaa gctttcatcc
    53221 caactccaac ctaggaaaaa gtgggggaga aggatcatta gaggaacagc aagcaaaatc
    53281 aattttacat aattattttc gattcgttcg ataaatgaag ctgtttgagt tggcgtattc
    53341 tgcgcttctt ctactaattg gataatctta gcaaagacag tttcggaact cgtttttgtt
    53401 actcgaatcg tgatgggatt tccttgattc agcgttcctg catacatctc atcgcctaaa
    53461 tatttttcta caggcacaga ttcaccgctt aatgcagact catcgatcgt tgcctgtgtg
    53521 ctttctaaaa agccatctat cggaacagct gctcctttag cgacaaatac caagttatcc
    53581 acctttagtt gagaaacatc tacttcttgc atttgtccat tttcttttaa taattgggct
    53641 ttttgtggtt gaagattcat caaagcagta atttcttttt gacttttatt agttgtgtat
    53701 tcctctaatg caccgcttaa acaaaagatg aatgtcaaca ttgctccttc aaagtaatta
    53761 ccgatcaaac aggcaccaat agcagctaaa gccatcaata aatccacatt cagtgtccga
    53821 tcattccaca attctttcaa gccttccgat gtttgtttat atccaccgat gataatggca
    53881 ctgatataaa aaagtggggt gatggtaaga gagaatcggt cgaagataaa accgaagatc
    53941 ataaataaaa ggcagccgat tgttgctgca aaagcttttt tctgattagt catcgttatt
    54001 agacctcctt gtcaaaacaa ggatagcata ataaaaatgt aatt
//
LOCUS       DAXWOO010000003        51083 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.003, whole genome shotgun sequence.
ACCESSION   DAXWOO010000003 DAXWOO010000000
VERSION     DAXWOO010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51083
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.003"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            290..1135
                     /locus_tag="ACGE70_000109"
     CDS             290..1135
                     /locus_tag="ACGE70_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFF8691783.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1416..2636
                     /locus_tag="ACGE70_000110"
     CDS             1416..2636
                     /locus_tag="ACGE70_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFF8691784.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2633..5353
                     /locus_tag="ACGE70_000111"
     CDS             2633..5353
                     /locus_tag="ACGE70_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFF8691785.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5337..6281
                     /locus_tag="ACGE70_000112"
     CDS             5337..6281
                     /locus_tag="ACGE70_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFF8691786.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6350..7360)
                     /locus_tag="ACGE70_000113"
     CDS             complement(6350..7360)
                     /locus_tag="ACGE70_000113"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFF8691787.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7540..7884)
                     /locus_tag="ACGE70_000114"
     CDS             complement(7540..7884)
                     /locus_tag="ACGE70_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFF8691788.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7877..8311)
                     /locus_tag="ACGE70_000115"
     CDS             complement(7877..8311)
                     /locus_tag="ACGE70_000115"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFF8691789.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8449..9186
                     /locus_tag="ACGE70_000116"
     CDS             8449..9186
                     /locus_tag="ACGE70_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8691790.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9179..10390
                     /locus_tag="ACGE70_000117"
     CDS             9179..10390
                     /locus_tag="ACGE70_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8691791.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10594..11376
                     /locus_tag="ACGE70_000118"
     CDS             10594..11376
                     /locus_tag="ACGE70_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFF8691792.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11446..12108
                     /gene="trmB"
                     /locus_tag="ACGE70_000119"
     CDS             11446..12108
                     /gene="trmB"
                     /locus_tag="ACGE70_000119"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFF8691793.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12206..13960)
                     /gene="ade"
                     /locus_tag="ACGE70_000120"
     CDS             complement(12206..13960)
                     /gene="ade"
                     /locus_tag="ACGE70_000120"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFF8691794.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14092..15021
                     /gene="comGA"
                     /locus_tag="ACGE70_000121"
     CDS             14092..15021
                     /gene="comGA"
                     /locus_tag="ACGE70_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFF8691795.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            14978..16027
                     /gene="comGB"
                     /locus_tag="ACGE70_000122"
     CDS             14978..16027
                     /gene="comGB"
                     /locus_tag="ACGE70_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFF8691796.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16024..16302
                     /gene="comGC"
                     /locus_tag="ACGE70_000123"
     CDS             16024..16302
                     /gene="comGC"
                     /locus_tag="ACGE70_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFF8691797.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16277..16747
                     /gene="comGD"
                     /locus_tag="ACGE70_000124"
     CDS             16277..16747
                     /gene="comGD"
                     /locus_tag="ACGE70_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFF8691798.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16719..17066
                     /locus_tag="ACGE70_000125"
     CDS             16719..17066
                     /locus_tag="ACGE70_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8691799.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            17029..17454
                     /gene="comGF"
                     /locus_tag="ACGE70_000126"
     CDS             17029..17454
                     /gene="comGF"
                     /locus_tag="ACGE70_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFF8691800.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            17426..17785
                     /gene="comGG"
                     /locus_tag="ACGE70_000127"
     CDS             17426..17785
                     /gene="comGG"
                     /locus_tag="ACGE70_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFF8691801.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17957..18964
                     /locus_tag="ACGE70_000128"
     CDS             17957..18964
                     /locus_tag="ACGE70_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFF8691802.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            18989..20173
                     /locus_tag="ACGE70_000129"
     CDS             18989..20173
                     /locus_tag="ACGE70_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFF8691803.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20610..22193)
                     /gene="abc-f"
                     /locus_tag="ACGE70_000130"
     CDS             complement(20610..22193)
                     /gene="abc-f"
                     /locus_tag="ACGE70_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFF8691804.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22298..22426)
                     /locus_tag="ACGE70_000131"
     CDS             complement(22298..22426)
                     /locus_tag="ACGE70_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8691805.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            complement(22771..22914)
                     /locus_tag="ACGE70_000132"
     CDS             complement(22771..22914)
                     /locus_tag="ACGE70_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFF8691806.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23137..23616)
                     /locus_tag="ACGE70_000133"
     CDS             complement(23137..23616)
                     /locus_tag="ACGE70_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFF8691807.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23689..23973)
                     /locus_tag="ACGE70_000134"
     CDS             complement(23689..23973)
                     /locus_tag="ACGE70_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8691808.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24051..24245)
                     /gene="ssrS"
                     /locus_tag="ACGE70_000135"
     ncRNA           complement(24051..24245)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACGE70_000135"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24317..25603)
                     /locus_tag="ACGE70_000136"
     CDS             complement(24317..25603)
                     /locus_tag="ACGE70_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFF8691809.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25741..26412
                     /locus_tag="ACGE70_000137"
     CDS             25741..26412
                     /locus_tag="ACGE70_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFF8691810.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26378..26866
                     /locus_tag="ACGE70_000138"
     CDS             26378..26866
                     /locus_tag="ACGE70_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFF8691811.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26879..27826
                     /gene="prmA"
                     /locus_tag="ACGE70_000139"
     CDS             26879..27826
                     /gene="prmA"
                     /locus_tag="ACGE70_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFF8691812.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27827..28591
                     /locus_tag="ACGE70_000140"
     CDS             27827..28591
                     /locus_tag="ACGE70_000140"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFF8691813.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28824..31037
                     /locus_tag="ACGE70_000141"
     CDS             28824..31037
                     /locus_tag="ACGE70_000141"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFF8691814.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSNLKSIVDKIKAVPDVYSVRRTNG"
     gene            31048..31494
                     /gene="dtd"
                     /locus_tag="ACGE70_000142"
     CDS             31048..31494
                     /gene="dtd"
                     /locus_tag="ACGE70_000142"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFF8691815.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31665..32924
                     /locus_tag="ACGE70_000143"
     CDS             31665..32924
                     /locus_tag="ACGE70_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFF8691816.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33013..33960)
                     /locus_tag="ACGE70_000144"
     CDS             complement(33013..33960)
                     /locus_tag="ACGE70_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFF8691817.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34354..35646
                     /gene="hisS"
                     /locus_tag="ACGE70_000145"
     CDS             34354..35646
                     /gene="hisS"
                     /locus_tag="ACGE70_000145"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFF8691818.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35661..37430
                     /gene="aspS"
                     /locus_tag="ACGE70_000146"
     CDS             35661..37430
                     /gene="aspS"
                     /locus_tag="ACGE70_000146"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFF8691819.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37539..38414
                     /locus_tag="ACGE70_000147"
     CDS             37539..38414
                     /locus_tag="ACGE70_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFF8691820.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38680..40563
                     /locus_tag="ACGE70_000148"
     CDS             38680..40563
                     /locus_tag="ACGE70_000148"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFF8691821.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40638..40922)
                     /locus_tag="ACGE70_000149"
     CDS             complement(40638..40922)
                     /locus_tag="ACGE70_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFF8691822.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEDAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40912..41646)
                     /locus_tag="ACGE70_000150"
     CDS             complement(40912..41646)
                     /locus_tag="ACGE70_000150"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFF8691823.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41775..42398
                     /locus_tag="ACGE70_000151"
     CDS             41775..42398
                     /locus_tag="ACGE70_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFF8691824.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42590..43711
                     /locus_tag="ACGE70_000152"
     CDS             42590..43711
                     /locus_tag="ACGE70_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFF8691825.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43718..46846
                     /locus_tag="ACGE70_000153"
     CDS             43718..46846
                     /locus_tag="ACGE70_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFF8691826.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46865..50059
                     /locus_tag="ACGE70_000154"
     CDS             46865..50059
                     /locus_tag="ACGE70_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFF8691827.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50205..50912)
                     /locus_tag="ACGE70_000155"
     CDS             complement(50205..50912)
                     /locus_tag="ACGE70_000155"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFF8691828.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16714 a         8934 c        10613 g        14822 t
ORIGIN      
        1 tttaaatctt ttgctaagtt gaactaatag agtcttttat gaatatcgaa gttagttttt
       61 cacctaactt cgttttttat agttggttat tataaaatag taatatagct gatttggatt
      121 caattcatat ttatttataa atcaaaaata cagagaatag tatataaatg tcctttccta
      181 ttcttaaaat gatgtttgta cttacttaaa ttaggtataa ttagtatatg ttttgtgaga
      241 aatttagact tacaataagc agtgaattta taatcaacgt ataggagaca tggatgaaat
      301 gaaactaatt ggtatagatt tagacggtac tcttttgaat tctgaccaga gcattagtca
      361 aaaaaatgca agaacaatga aacatttacc ggaagactgt tttccattca tttgttctgg
      421 aagagaagta gaagacatta ataatattct caaaaaatct ggtttaagga taccagcagt
      481 agggttaaat ggttctgttg gatttgatgg agaaaaaaag atatttgagt cttccttcga
      541 taaaaatgat gtaaaaaaaa ttgattctgt cctatcgaaa tttccaacca aaatatatac
      601 aaatctcgga agttatgaat ctcagaatta taaaaataaa atgaaaaaaa tttttcacga
      661 ggtagggagt gaattttcta tcaaagagct aaactatgag cttgagtatg aaaaatctat
      721 ttgttcaatc ccttataata atattgagga aattatagaa aaggatgcta tcagcattta
      781 caaatttttt atttttattc ctaatcgtca attgaaagct aatatattta accatttgaa
      841 aaagatgcta aatgtgtctg ttactgaatc ggcacctgta aatattgaaa tcgtacctca
      901 aaatgtatct aaaggtgtgg tgtttgatcg tttggaatca atctacaatt taaagaaacc
      961 tttgagaatc gcgatcggag atagtttaaa tgacttatct atgtttgaaa gtgcagatat
     1021 tagttttgca atgggaaata gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt
     1081 ttcaaatgat aaagacggag tatctcaagc atttgaaaga attcttacca tataaactaa
     1141 ttaaggtagt atagaacata gaattctaaa tacggaaaaa tggagcataa attccagtac
     1201 aaatagtact tcttcagtgt caaacgaagc gaatataaca tagatattta ggattgtgac
     1261 aaaaatcatg tcacaatctt tttttcgaat aaacttattt tttgaagtcg gccatttatc
     1321 gtaatttctt tgcataatac gaacaaattt tcggttattg atgaaaaaaa acattgaaaa
     1381 tggtatgata aaagaaaaaa aggaggaaat ttttaatggt gaaatttatc catgcggcag
     1441 atttacatat ggatcggtca tttgaaggac tgacaactct tgataaaact gtacaggaaa
     1501 aactattgaa gacgaatctg accgttttat caaatatcat tgaacaggca ataataaata
     1561 acgttgattt tgttttactt gcaggagata attttcatca aaatcgtcct tcattaaaaa
     1621 tacagaaaca tttttcagaa cagatgaaac ggttagaaaa gaaccatata cctgtattta
     1681 taatattcgg aaatcatgat ttctatcaaa aagagcggta ttggttctcc tttcctaaaa
     1741 atgtccattt atttacatcc gaaacggtag agacaaaaaa aataacgata aaaagtggag
     1801 aaactgtctc tctcagtgga tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga
     1861 tggaatttcc ttctagagaa ctcacagatt accatattgg gttgtaccac ggggaacctg
     1921 gcgtctctca aaaaggaaac tatgctcctt tccaaccctc aaagatgcaa gaaaagggat
     1981 acgattattg ggctttaggg catatccatg tgccaacggt cttaaatgaa aagcaaacaa
     2041 ttgtttatcc aggtgcgcct caaggtcata ctaaaaagga acaagcttct acatctattc
     2101 tactagttga attaagtaaa ggaagttgcc aaatacatcc gattaaagta gcagaggtct
     2161 attggaagac aaaggaaata tcattgagga aagcaagaac gacaaaagag gtcattgttc
     2221 acattcgtca gcagctttca aaagtagagg atggatttag tttaattgag ataaaattaa
     2281 aagattatga ccatctcaat acagatgttt tggaacggat acaatcagga gagctattag
     2341 actatttgtt agaggaattc tcagatagga taaatgattt ctttatttgg cgtatttctc
     2401 tcatagagaa tactttttat acgaagaccc cacttgctgc ttctgctaaa ttgatggaac
     2461 agttatttca atactatcag acacctcaag atttccaaca aattttaaat gaagtatata
     2521 gtcatcaaga agcggctaga atattgagcg aactgccgga atatcaagaa gaaacacttg
     2581 aaaaagccaa gcagctgctt aatcaagatt ttttatttga ggaggatcag gaatgaagat
     2641 tttaaggatc gagattgctg cttttggaaa atggcgtcaa aaaagttttg acttttattc
     2701 aggcaatcag ctgatctatg ggggaaacga agcaggaaaa tcaacgattt atcaatttat
     2761 tcaagcaata ttatttggtt tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa
     2821 agacggctca gcctatggcg gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc
     2881 tgtcgagcgc tacaagcagc aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt
     2941 gggaagtgat gagttactag aaaaactgat cgcacctttg acgaaagagt tattccagca
     3001 ggtatttact ttccagcaag agcagttgat ggaactagat catttgagag aaaaagagct
     3061 acatgatgcg ctgatttctt taggcatcac gggcagttct caagtattcc aaaagcggca
     3121 agaatattat cagaaagcac aacaattatt caagaaaaaa ggccaaaaat tactaatcaa
     3181 tcaaaaatta aatgaatgga aagagctgca agaaaaaatt catcaaaaac aagaacagga
     3241 agcttatttt ggcaagttga tccaacaaga acaggaatac gagtcgcaac agcagcagtt
     3301 attggcacaa ttaaaagagg cgaacaaaca attttcatta gccagacagc aagaacttaa
     3361 tttttcttta tatgaagaat ggcaatctct caaaaaaaac agcactacag gtcttcctga
     3421 tgagcaactt cttttagatc tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg
     3481 tttggaagaa ttagaagcaa cactggaaaa gcatagcggg atggatcagc agtcagccag
     3541 atattatttt tatttagagc aggagcagga actaaaagat ctccagcaac ttcaatttac
     3601 agcggaacaa ttaaacgaag aatggcaaag attacaaatt gagactagag aaacaaattc
     3661 agaattgaag attttggaac aaaggtggca ttggcagaaa aatccgttac cggaagaatt
     3721 tttatatgaa gaagaatgga agcatcttct ggctgaagaa agaaggattg ctgatctaga
     3781 aagccagatt tctgtgcgtc tccaaatagc taaagaacag caagagatga ttgaagcaga
     3841 agtgaatgac ttggaagaga cctatccagc attattacaa aatcagtcag agatagcacc
     3901 agtctcttca tctaaaccgt atatgctgat ggctggcgga gcaataatca ttgcaggtat
     3961 atttgtacca atgcccttga aagtgatcct tttactaatt ggtgcaggca tgattggcta
     4021 tttcttctac aaaaggcaac caaaaattaa aactaaaaat gatttctctg agataaaagg
     4081 aatgtggcaa gaaaaactag gacaacttga tctagcctta gctcaattag ctgaagcaga
     4141 agaaaaacaa aagagaactg gagaagaaaa acagattctt cagcaagaag tatatcgttt
     4201 tgcggatcat cggcacttag gaaagatgga tacgctcaaa ctcatgaaag aatatggaca
     4261 ggaagtcata gattatcaga aattatatgg cgtatttgag cggaaacata aaagagaaca
     4321 ggaaatacaa gaaaaactac gacaaatcga ccaaaaattt gagtttgtta aagaatggtt
     4381 accgatacaa gataagacaa tttcagaaaa aatggtattg ctttcccgtt ttatacaaga
     4441 gatggaacag atgaaatttg ccagaagcta tcagcaaaat actttattga agcaagaaat
     4501 caatcaattg aaaaagaaac aaaaagtatg gatacaagaa tacagcgaac aattgactca
     4561 agcaggctta tcttatcctt cggaagtacc tgtcttttta caaaaagcca aacaagccga
     4621 acagcagatg aaaagataca atgaattatc ggaaatgctt actccactat ttcctgaaga
     4681 gctgacaaaa gagaaattat ctgtaagact gcgccaactg aaagaaaagc aatcccaact
     4741 ccagcaagaa gaaaaagaag caagtgaaaa tcggcaacga ctccaacttc aagtagaaga
     4801 gttgcaaaga gacggcacat tggacgaact ttatcaacaa gctagccggc aaaaagcaaa
     4861 attacaggaa ttactggagg aatggggagc acttgaaatc gccgggacat tcttaggtga
     4921 tctttcaaca gagatgagtg aaagacaatt accccagttg ttaagagcag caggtgatta
     4981 tttaggtatt ttaacaaaag gaaattatca gaagatatta ctgaaaaatg atacactgac
     5041 tttaacagac ggatatacag aatttcccat ctatgagtta tctacaggta cgaaagatca
     5101 gctgattatg gcgatgagat ttgcttacct ctcattagaa gctaaccgtt cgatatgccc
     5161 gatcatcatt gatgatggct ggcttcatta tgatcatcag cgaaagtatc aattagctga
     5221 acttttagct aaatttggag aaaagtatca aataatttgt ttttcttctg ataaagaaat
     5281 ggtaagctat taccatgaga tgaaccaaat cgtaaaacaa ttagaaggag caaataatga
     5341 aaaaaattcg tgatttagta atcgatgagc agtttgaaca atttgtcctg atcaaaagtg
     5401 cagatgtaag aatcgctaaa aacggaaaaa aatttattgc ttttactttc caagatactt
     5461 caggtactat cgatggtaaa tactgggatg cttcggagga ggaaatcaat aaatttacag
     5521 ccggcacagt cgtttttcta aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa
     5581 agattcttca tatgcgctta acaagggtag atgagccagg agagcctagt ttatacatgg
     5641 aaagagcccc tttgaagaaa gaagaaatgg aagaagaaat caatcaattg ttgtttgaga
     5701 tcaccaatgc gcattggaat cgtattgttc gctatctact taataaatat caaaaacaat
     5761 tttttgagtt tccggcagca aaaagaaacc atcatgcatt tgccagtgga ttagcttatc
     5821 atactttgac aatgcttcac ttagggaaat caatcgccga tgaatatcct gagctgaatc
     5881 gagcattgtt gtatgctggt atcattttgc atgatttagg aaaggtgatt gaactttccg
     5941 gtcctatgtc gacagaatat acgcttgcag gaaatttgat cggtcatctg gtattgattg
     6001 acgaagaaat aactaaagcc tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc
     6061 tcgtattacg tcatatggtt ttagcacacc atggcttatt agaatatggc tcacctgtac
     6121 gtccaagaat aatggaagca gaaatattac atcaaatcga caatatcgat gcttcgatgc
     6181 agatgatgtt gacttcgatt cgtcaaacag aaccaggaaa atacaccgat cggattttcg
     6241 gtatggacaa ccgaagtttc tatgtaccaa aagatttata gcaaaaattt atttttttca
     6301 ttcaaaacaa aaaaagcgag acataagtct tcgctttttt tgttttcttt tattttgatg
     6361 aatcagttgt ttcttcagtt gaagattctt ttgttgaatc agttgatttt gtgtcagaac
     6421 tctttgttga agctgtagtt tcacttgagt ctttcgtact tgatgaagtt gttgtgaatg
     6481 cactcaacac attttcaaag gcatcatctt tgatttttac atttgcatct tttaattctt
     6541 caccgattac tttagttgtg aatgcattgt cacttagttt agtttcagta gcgatatctt
     6601 ttagttgatc tttgtattta tccatgtcat tgcctttatt ttggtttttc accattttga
     6661 caacgtagta ttctgtcgca taagaagtag gatttgttgt agtaataaca tcggagatct
     6721 caccgtcttt caatttgaaa gctgcttctt ttacttctgc aggaatagtt gttgtcgttg
     6781 agtcgaattt aactttaccg ccatcttctt ttgtctctgt atcagttgat ttgtctttgg
     6841 ctaatttaga gaaatcatct ccatcatccg ctgatttttt cacatctttt gcttcatctt
     6901 cacttgataa tttgatgatt tgtgcttcta cttctggatg gaaagatttc caagcagttt
     6961 tcaaatcgtc atctgtaata tctacgtgag ctttcaagcc agcttcaaaa gctaaattgt
     7021 tacgaatata ttctttataa gtatctttcg tatatccagc agcttctaat tgtgattcaa
     7081 aagtatcacc taatgatttc gcttgtttgt cgtattctgc atccacttgc ttatcggtta
     7141 ctttatcgcc atatttgtta ttgaatactt tataaatgat catccgttgt actaaggatt
     7201 ggttcgatga ttcaagtttt gcttcatcat agaaatcaga tacagtgatc gttccgcctt
     7261 tcatcgttgc gatatcttta ttagtatcgc ctgaacaagc tgctagagtt aaaacagcca
     7321 acgcactagt agctgccaaa ataattgatt tttttttcat taaaatttgc actcctgtaa
     7381 ttagttatta tttttatata ttaactatac catatcccat ttcctttaaa aacgttgtcg
     7441 tggtagtttc aaacaaaatt caaaatttca tcacattcct aggaaatacg caaacttttc
     7501 tcacttatat agttaaatct gttgagaaaa taatcattat tatatttgtt caggatactc
     7561 tggcagatca gcttttagtt tttctacttg ttcattgatt tgtgtgatac gtggttcggc
     7621 ttggaattta aaatcttcga tatctttttt gattgcttct tggaaagcag gaatcacaga
     7681 ttcagctgtt tcttttgttt cgatcaatgc gcttttaaaa tgattcaggc tgttatttaa
     7741 atcatttgtc agagttgttg cttcttccaa ttcttcgatc agtttctttc ttgtttcatt
     7801 cccgctcctg ggcgccaata acagacctcc gacagtgcca gcagcagctc caaataacag
     7861 tcctttgaaa aagtttttaa tcatcgcagg acacctgctt tacgattgtt tcttgaatag
     7921 cttttagttc ttccgatcca taactatctt gatgattgac aaagtgaata gaaaaatcat
     7981 cctctttgct gtagcgaggg actaaatgga tatgtgaatg aaaaacagat tgataggcta
     8041 gttcacggtt gttattcaag atattaagac ctttcatttc tggaaaagct ttttccaatg
     8101 cttgagcaat cttaggcaca cgcgaaaaaa cctctcctgc aagttcggga tcatattcaa
     8161 aaatatctgc tacatgacgt ttaggcacaa ctaatgtgtg tcctttcgtt gcttgagaga
     8221 tgtctaaaaa agcatagacc ttgtcgtctt catatatttt atagcttggg atttcttgat
     8281 tgatgatttt gcaaaaaata cagttttcca tataaaacat cctttctata aagaataaat
     8341 acttcagctt aactttacca taagttaaag ctattgtaag gaaaaaaaca aaataccgtc
     8401 attttcttcg tggtataata aataaaaata aatggcggag gaaaaagaat gagtttaatt
     8461 atcgatcatg tcacaggggg gtacggacat ctccctgtgt taaaagatat cagtttcgat
     8521 gtacagcctg gagagatagt agggttgatt ggactgaacg gagcagggaa aagtacgacc
     8581 atcaagcaca ttatcggtct gctggaagct tcaaaaggaa cgatagaaat caatcatcta
     8641 tccttaaaga aaaatacgga agaatatcgc aaacaaattg ggtatattcc agagactcct
     8701 tctttatatg aagaattgac attgagagaa catatcgaag tcactgcgat ggcctacgat
     8761 atacctatgg atatagcaat gaaaagagca gaaaagttgt tggagacttt tcggttatct
     8821 aatcgtcttg actggttccc agctaatttt tcaaagggaa tgaaacagaa agtgatgatc
     8881 ttatgcgcct ttttgatcga gccttcttta tatatcatcg acgaaccatt tttaggcttg
     8941 gatccactag ctatcaatgc attgcttgaa ttgatggtag aaatgaaaaa agaaggagcc
     9001 ggtattttga tgtctacgca tattttagct actgcagaaa aatattgtga tcgttttgtg
     9061 gtcttacata acggagagat acgagcagac ggttctcttg agatgttgag aaaagaattt
     9121 cagctgccgg attcttcttt ggacgaaatc tatattgctt taacaaagga gcaggcacat
     9181 gagtagtttt tttcaaaaac gtttagcaag acatcaaaag aaaatgatga aatatatgcg
     9241 ttatgtattg aacgatcatt ttgttttagt ttgtcttttc ttgattggcg gtgtgggatt
     9301 atattattct aattggttga aaaccctgca gccaccgttt gcaattggag ggctcataat
     9361 ctctgtattt tggatgctct gtttattcgt gggtaagatg gctacttttg cagaggcagc
     9421 agatattgtt tttttacttc ctaaagaaaa agaaatgcaa acctatttgg aacgagcttt
     9481 tcgctattct tgcatttttc cgttgatctt tttgatattg gcatgcggat tggccatgcc
     9541 attagtggtg gttagtacgg agcaatcctt ttcgatgttt ttcgtctatc tgatcatatt
     9601 atggagtttg aaattcagtc atctgcaaat caagagagca gaattgtttc gactagatca
     9661 acaagttatt cgttattgga aattaggctg gtttttttct tctctgatct ttttgtttct
     9721 atcattttat tggctaatat gggcaggaat agcaggtgca ttagtacagg caagtctata
     9781 ttactggttt ttgtggaagc aacaagaaaa aggattggat tgggagaaaa tggtgcatgt
     9841 agaagaacat aggttgcatc agatctatca atttatcaat ttattcacag atgttcctga
     9901 aattacggca aagatcaaaa gaagacgcta tttggatcct atattgactc atattaaaag
     9961 agaatcaaaa aacacttatc tttacttgtt tgcaagaaga ttggcaagag gttctgattt
    10021 tagtggtctt tatttccgat tagtaattat aggtgggtta ttgattgccg gagtagtcga
    10081 cttttatttt agtctgatta tcggtttctt atttttatat ttgatcggtt tccaactgtt
    10141 acctttagcg aaccagttcc gctatatggt tctcgttcag ctttatccaa tccataagaa
    10201 tcagaagaag caggcgattc agtatctact cacatgggta ttaattatcg cgtcggttat
    10261 ttttggtttg attgctgcag tcgtattaga tgggcagcag cgatgggttc cgctagcggc
    10321 ttatttgatt gttactatgc tctttgttaa actatatgta ccaactcgtt tgaagaagat
    10381 gtcagactga aaacaaagct gtgtagggta aaaatgagaa tgaaaaatag agcgttgaca
    10441 gtaagaatgc tgttcgttct attttttttg agaaaagctg gttttttgaa tggcaattaa
    10501 atgaaaaaga acgtatatgt gcttgacgtg tagtgttcag gcgactagaa taataagtac
    10561 tgcaaaacaa agagtaagag gacgggagaa tgcatgatgg agtttcagtt agaccaagag
    10621 tggcggttac gtcccatcaa aggcgacaca ggaaaaacat acataggcat gcgtgatgag
    10681 gacaaagtct ttattaaacg aaacacgact ccgatgttgg ctgctctttc aagggaagga
    10741 attacaccaa aacttgtttg gacaaaacga acggggaatg gcgatacttt gacagcacaa
    10801 gagtggctgg aaggtcgctt gctgactcct aatgagattg gtcaaagaaa cgacgtgatt
    10861 gatgttttgt accatttaca tcattcaaat tctcttaaaa ccatgctgac ccgtatcggt
    10921 ggtcgtgtca tgacgccgga gaagatgctg caaaaatatg aaaaagaatt accggaagaa
    10981 ctccagaaaa atcaatttct gcaaattgtc catcgttttc ttcgtgagca gatcccgtca
    11041 atagataagg aaaacttatc agtagtacac ggagatgtaa accatcgtaa ttggttagta
    11101 tgtaaaaatt atttatatct agtcgattgg gattcaatca tgtttgcaga ccctgcggtg
    11161 gatattgcaa cgattttagg aaattatgtg ccactgtcta gctggaatca gtggctgatc
    11221 agttatggca tacgcccgac tgacgaaatg cttgaaaaat ttcattggta tgcggccatg
    11281 aatttgctcc aagaaatcac cagatattgc aaacgtgaag atcatcgacg gatgaacgaa
    11341 gaaattttac aattaaaacg aattttcagt ggctgaggtt tggcccaaac tctgcggaga
    11401 tttggccaga cctttttaat tagaggaaag gaatggagat aaataatgcg tgtccgaaat
    11461 cgtccgggag cagcggaaat gttagcggct cacccgaatt ttgtaattag tgatccaaca
    11521 ttgtggaagg gaaaatggaa tgaattgttt gaaaacgatc atccaatcca cattgagatt
    11581 ggtatgggga aaggccagtt catcactgga atggcaaaag cccatcctga aatcaattat
    11641 attggagtag aaatgcaggt cagtgttgtt tcgattgcac tagacaagtt gatcgaacaa
    11701 ccgcttccta acctgaaact tctacatgtg gatggatcag cgttgacaga gtatttcgct
    11761 gacagcgaag tagatcaaat ctatctgaat ttttccgatc catggccaaa aaaacgtcat
    11821 gaaaaacggc gattgacata taagactttt cttgctgtag atgaacagat tcttcgtcca
    11881 aatggagaaa tccatttcaa aacagataat caaggattgt ttgagtattc tctggcaagt
    11941 ttttctcaat atggcatgat cctcaaacag gtatggttag atttacatca aagtcaattt
    12001 gaaggcaata ttatgacgga gtatgaagaa aaattttctt caaaaggtca acgtatttat
    12061 cgagtagaag ctcaatttca agataagacc aaaaagaaaa gtctgtgaaa ctaactttct
    12121 gctttcataa aaacattaat atccataaat aaatcccaga aaatgaacat tacagatgta
    12181 atgtattttc tgggatttat ttgatttaaa atgtatttat ttcatcggtt tttccacaag
    12241 gtggaatcaa ttggaagcga gaaaatgaaa ataatccttt gtcggtgatc ttcaattcgg
    12301 gtatgacaga taatgttaag aaagaaagtg tcaaaaaagg atcaaaggtt ttagctgctc
    12361 ctagttgatg tgctttagtt attaattcat tcaactgttc atagacgatc gaaaaaggtt
    12421 cctgtgtcat gattccgcct ataggaagag aaacacaagc tatttcctgc tgtttgttga
    12481 caacggtcat cccgccacct ttttgaatca aaagattagc agcatacagc atatcctcgt
    12541 cgtttgtccc aacgacaact agattatgcg agtcatgggc aatcgttgtc gctatcgctc
    12601 cttgtttcag ctgaaatcct ttcactatgc ctaatccaat gtttcccgtt tgatggtgac
    12661 gctcgatcac cgcaatcttt aattggtctt tttcgattga aggaatgaat cgttcgcctt
    12721 gtcggtccac tttttctacc aaatctttag tcactagact attcgggata atttcgatca
    12781 catgggcttg tgaagaagaa agaagcagct ctaacgaatg tggatttaat ggttgagcgt
    12841 ttagttttgg cagacaacac gtatatgaat ttgaatgatc agcaaataaa tggtcttgga
    12901 gttgcccaga ttgaacaact aatgttccct tatagaaaac ttcatgaatt ggcagcttgg
    12961 taagatcgtc tgttaagaag aaatcagctt gatacccagg ggcaatcgca cctagtgttt
    13021 tcaaatcaaa acattctgct gcattcaatg ttgccatctg aatggcttgt aatggatcga
    13081 ttccaagtgc aatagcttga cggacgatat gatcgattga tccttctttt actaaatcat
    13141 tgatcagttt atcatcggtg acaaagaagc atcttcttga attttcaggt gtgatcgccg
    13201 gtagtagcga tgccagatct tttgcaacgg tcccttctcg aaccattaaa tacataccaa
    13261 gatccaaacg ttctttagct tcttttgcag ttgtagcttc atgatccgtc cgaatacctg
    13321 ctgcaggata aacatttaac tcttccatgc cgataccagc tgcatgcccg tcaatttttt
    13381 tcttatgctg gtgcatcaat tggatctttt ctaggatgtc tgtttcatta tttgctactg
    13441 cttgatagtt catgacttca gctaagccta aaacttcggg tttttgtaaa aaaggggcta
    13501 atttttttgc atttaaattt gcaccattgg tctcaaaagg ggttacaggt acacaagaag
    13561 gtagcataaa aaagatattc atgagtgttt gtcttgcatc ttctatcata aactcaagac
    13621 ctgtactccc agcaatgttt cctatttcat ggggatctgt cactacagta gtaactccat
    13681 gaagcaagga tacttttgaa aattcgcttg gtgataaaag tgagctttcg atatgcacat
    13741 ggccatcgat gaatcctggc actagatatt gcccattagc attatgtacg ctttttcctt
    13801 cgtattcacc gattcctacg atcattcctt cgcagattgc tacatctttt tccatgattt
    13861 ctttcgtaaa gacgttgacg atcttagcat gcttgatcac taggtcagca ggcttttttt
    13921 tacttgctac ttcgatatat ttccttaatg ttgtttccaa tggagtgcct cccaagataa
    13981 atttttataa gtataacata aattcggttt ttgccaatat ttttttgtaa attcatttta
    14041 tttgaaacaa aaagatcaaa aagacgtatg ttttttgtgg aggggagaat aatggaaata
    14101 aaacgattat cggatcaaat gataagtgaa agttttgcca aacatgccca agacgtatat
    14161 atattgccag cttcagaagt attttacatc tattttcgct atggaaaaaa acgaacattc
    14221 tggaagaaag tctcttcggc agatggacag caactgatct caagatttaa gtatttggga
    14281 cagatggata tcggagaaaa aagaaaagct cagttaggtg catttactta ttctttagaa
    14341 aaacaggaag ttcgtctgcg gttgtcttct gtaggagatt accttcaacg agagagttta
    14401 gtgattcggc tattatacgg tacttttgaa acaccccatc gttgctttag ttctcgagat
    14461 tttgcaacta ttttacagaa aaccgtcaga cgtggattat accttttcag cggaccagtc
    14521 ggatcaggca agacttctct aatgtatcgg ttggctttgg aagaaaaatt gcaagtgatt
    14581 actatcgaag atcctgtaga gatagaagaa ccgttttttc tgcagctaca gatcaatccg
    14641 aaaatagcac agacttatga tacacttttg agactcgcat tacggcatcg tccagacttg
    14701 ctaatcattg gcgagataag agatgaatca actgcacatg ctgcggtaag agcagcacta
    14761 acagggcacc gggtttttgc aaccgtacat gcacgcaatc ttgaaggtac attggtcaga
    14821 atccatgaac tgacaaagaa aaaagaagag ctatctgaat gtttagctgg tgtcatttat
    14881 caagaacttt tggaagatgc caatcaccat ccttctgttt tatggagtta tcaatttatg
    14941 gaggaagaat cagtaaaaaa atcatgggag gaaagttatg aagaagctca ggaaaatatt
    15001 tggcagtcag aactattcta aaggattaac aagaagccag caaaaccagt ttgcacaaac
    15061 catgggagaa ctgcttttat ctggttttag tctgcaagaa tccctttatt tattactcac
    15121 gatccgttcg tttccacctg ttattttggc aaatatccaa catcagctaa aggaagggca
    15181 agcgtttagt gaggtacttt cgcaaatagg atatggagca gagcaactgg tacagatcga
    15241 gttagctgaa cgtcatggga atctgccgac aactttgttg gaaattgcca aacagtttcg
    15301 gcttgttgaa ggatttcgca atgatttgaa aaagctgctt gcatatccga tgctattgct
    15361 cacctttttg ctaggtatct tgtttagtat gcggttgttc gttttgcctc agcttattgg
    15421 ttcaggaatg gtcatgcaag agcattgggg tattcagctg atacaagtct ttcattggta
    15481 tgttctacta ttcatcctag cggttactct attggcttta ctagtaaggt atcgtctgat
    15541 gaagttgtct cctgttgatc agagtgaatg gataagcaga caattattga ttggaccgat
    15601 atattccttg tatcaatcgg cttatttgtc actagagatt ggaaagcttt ttcatgaagg
    15661 tttcgaatta aggcaaatca tagtctgctt aaaggaaacg aaacaagaaa gtctgattca
    15721 gcatcttgcc tctaagttga ttcaaggatt ggaggcaggt atgtcattgg ctgagcagtt
    15781 ccagaattat cgttttctat cagaagaatt cagtaaaatt gttttacagg gagaggcaaa
    15841 gggaagttta ggtaaagaac tgatttttta cagcgaatgg acaagaaaac agcttttcaa
    15901 acgtcttcat cactggatgc attttattca gccaatcatt tttttgggcg tagcggcttt
    15961 gattttatta gtttatgccg ctgttttatt acctgtttac ggaagcatag aggaggtttt
    16021 gccatgaaaa agagaaaaat atcttattat agcggtttta cattgatcga aatgctaatc
    16081 gtcttactga tcatcagtgt attagtgttg ttgttcgtcc ctaatttgtc gcgttatagg
    16141 aatcatgtag accaagaaag tcgtgaagcg atcatccagt tggtagacac acaaaaagag
    16201 ctctatgcgc ttcaaaataa tggaagagtt cctactgtgg aggaattgtt gaatgaaggc
    16261 tatatcaaac gagaacatgc agaaatctat cagcggcctt gaaggtaagg ttttagcatc
    16321 aggatatacg ttgtttgaat cactcttact gattttatta cttacaacga tcctttcttt
    16381 tccagttctc gcattttctg catggaagaa tgaattggca gtctatcaat ttttcggcca
    16441 atttgaaaaa cgaatctatt ctacgcaaaa aatcgctatt gtgaatcaga tccaaacagg
    16501 cttttattgg aatagtgaag agaatcaaat catttttcat gttccgaatc cagataaaat
    16561 aaattgggaa gtattagata ttcctgatga gattaccttg aaacgccatg cttctattac
    16621 ttttgctgct agaaccggga atgaaagttc actaaaagct tatcaatttt attgggaaga
    16681 aaaagagcgg acgattacgt atcaattcca gatagggagt ggtcgctata caaaaagaat
    16741 tgaataaaac aggatttgtt ttgttggagt cccttacagc gcttatgatc gcttctgtct
    16801 gtgtctactt agtgagcttt ggccagctgt ttttgcttca ggcagaaaag aaaagtgggg
    16861 aggaactgct atttattcga atgatttatg aagatatcaa gacgcagcgg atgtatcaca
    16921 aaagaccaga gcgaagggaa gaaatgatcc catacgatat ccaaattgtc gataataatg
    16981 gagtaatcga aaaagtgttt gtcaatatgg agaaagaagc gatcagtatt gaaaaaacaa
    17041 gtaattccag catttacgct gattgaatgt ctaattggac tgatgatatt gagcagtcta
    17101 ttgctcacat tcaatctttt gattcagcaa accatccaag taagtgaatt tttgcagcgt
    17161 aaagatcaaa aagagtggct aatattttta gcacaattag aagaagaact caagagcagt
    17221 cacaatgtct ctgtgaaaaa tcagcaactg cactacacga tcgaggataa acagtatatc
    17281 atcgaaagat atcaggcaat gatacgtaaa agaagaactt ccggcggaca tcaacctatg
    17341 ttaacaagta tcactgagct tcaattattg gaaaaagaac acacaatttc tttttatgta
    17401 cactttgaaa atggagaaga tggatatgcc atatggacaa aaaacgatca gtagaaaatg
    17461 ttataagggc tctattttat tttctgtctt gcttttattt cttttatgta cgtttttatt
    17521 gatggcagca ttagaaagct accgtttgtc aatggatctt aatatacgaa tcaaaaatta
    17581 ttatatagca aaaataatga agcagatggc tttatcggaa cagtctacgc ttgccgaaag
    17641 ttcggaagga gttttttact acacaactgg atctgtaaca tatcagtggg tacaacagtc
    17701 cctattctta gaggtggaag tctcaccgtt tatctttcgg ttcacagaag aagtaaaaaa
    17761 tgatacagat actagtacag aatgatggaa tgttcgtgaa aacgccctct tttctgttat
    17821 aacactgttt ttaataaaaa aatgtttgcc gtttcacaaa taaaaaacag tgaaaacgat
    17881 tgaaattttg tcaattgttc gatagaatta aacaggtgaa aaaattgtta taatatatct
    17941 agaaatgagg tgcaccatgt taccagaaaa aatggaacaa gggtttaatc aaaacctgga
    18001 agctattcag cttttacaga atgcattggg aacttccttt ttagaagcct atgtcgaaaa
    18061 tgcagaaaat ttaatagatg attatcaagt acgtgtggtg gatggagtgc cgacaaaaga
    18121 gacaacgcaa cggatcacag ctctttatga agagttgaag aaactttctt tcgagccaga
    18181 agaatggcgc agactgtctc aattactttt attgaagggc agccagacag aacatttgca
    18241 ggcgaaccat caactcactc ctgatagtat cggtttttta tttgtttttc ttattgaaca
    18301 actatacaca aataaaaaag caccggttaa gattctggat attgcggcag gaatgggcaa
    18361 cttgttatta acagttcttt taaacctatc gaatgcgggg taccaaacag aaggaattgg
    18421 tgtggatatt gatgatacat tgttggctgt cgcagcttct acaagtgatt tgacacaagc
    18481 aaatgttcaa tactttcatc aagacgggtt gcaggaatta ttaatagatc ctgtcgattt
    18541 tgcaatcagc gatttgccaa tcggttatta tccgaatgac gaaaaagcaa aagagttttt
    18601 aacaagtacc gaagaaggcc atagttatgc acatcattta ttgctagagc aatcaatgaa
    18661 atatgtgaag ccggatggtt ttggtttgtt tttgatgccg agtggatttt tagaaacgga
    18721 tcaaagtgaa gaaatcaaaa aatggtttaa agaagaaggt tatcttcaag gaatgattca
    18781 attgccagat gaattgttcc gtaataaaca atcgcaaaaa agtattttga tcttacagaa
    18841 aaaaggacca caagcacaac aagtaaaaga agtcttgctt gtgaaacttg cttcattaaa
    18901 agaaccagag aaggtgacgg aatttttcaa cgagtttaaa aactggaaat catctgctct
    18961 gtaatcattc aacgagaagg agagtcatat gtctaaaaca attgcaatca atgctgggag
    19021 ttcaagtttg aaatggcaat tatatcaaat gccagaagaa accgtagttg cgaaagggat
    19081 cgtcgaacgt atcggtttac aagattctat ttttaccatc aagtacggcg ataatcaaaa
    19141 attcgaacaa atcatggata tcgaaaacca cgaagtagca gtaaaaatgc tgttagatca
    19201 gttgattgaa ctgcaaattt taagctctta tgatgaaatc accggagtag ggcaccgtgt
    19261 tgttcacgga ggagaaactt atggggattc agtagtgatc gatgagaaag taatggaccg
    19321 cattgctgaa ttagcagaat ttgctccttt gcataatcct gcaaacttaa tggggatcaa
    19381 agcgtttaaa aagattttac ctgacatttt gagtgtggct gtattcgata catctttcca
    19441 ctcaacaatg ccaaagcaaa attatttata tagcttaccg gtgtcttatt atgaagattt
    19501 caaagtaaga aaatatggct tccatggcac aagtcatcgt tatgtgtcgg aacgtgcggc
    19561 aaaattattg aacaaaccga tcgaagaact aaaaatcatc acttgccact taggaaatgg
    19621 tgtatcgatc acagcagtcg atggaggtaa atccatggat acttctatgg gattcacacc
    19681 attagctggg gtgacaatgg gtacacgttc aggtgatatc gatccagctg ttctcccata
    19741 tttaatggaa aaattagaga ttgatattga tgaaatgatc aatgttttga acaagaaatc
    19801 tggtctgcta ggcctaacag gattgtctag tgatatgcgt gatcttgaaa agaactatga
    19861 aaaagaacat attcgtttag cttatgacat tttcgtggac cgcatccgaa aatacatcgg
    19921 cggttatgtg acaacgatga atggtgttga tgcaattgtc tttactgctg gtatcggcga
    19981 gaatgacgga catgtacgta gtgaaatcat caaaggtatg tcttggtttg gttgtgaaat
    20041 cgatccagcg ttgaatgaat tgcgtggtga agaagtagat atctctacaa aagaatcaaa
    20101 agtaagagta ttagtgatcc caacagatga ggaactaatg attgcccgtg atgtggagcg
    20161 tctgcgcggc taagccaaag cattcgttag gataaaagat gaaacgcatg caagagggaa
    20221 ttcttgcatg tgtttttttt gcggagaaga ccgcaaaatg gtaatttaac ttcaatttcc
    20281 cttctttttt acaggatctt cttagggaat tttgcttgga aaagtggtca aaagggaaaa
    20341 gactggcatt ttatttattg attttcagga aaatcccaat attttatttt cattcatctt
    20401 tataagggac attacatatg taaaaagaaa aaacattttt agatcagcct cgtgggctgt
    20461 cgactttatt cttcgcttta tgtttagcta gaaaaccgat ccgaaacatt cattgatcga
    20521 ataataaaaa cttgattttc cacctgtact ttgcctgaat cgtgaaagca caggtatgaa
    20581 aatcaagttt tttgttgcga aaaaacgtcc tagtcaatca gttggccgag cggctggcgg
    20641 ctgatctgct cctgatcaat aacgagtgct tcttggaagt gttgctgcaa aaagtagcgg
    20701 tcatggctga ctgctaggag cgttccttga tagtcttgca gcatttcttc aatttcttct
    20761 cgagcatagc tgtcgaggtg attggttggt tcatccaaaa tcaggacgtt gattttttct
    20821 aaaaagagtt tcagcaacgc caaacggatt ttttcaccac cggataaatc tttgatccgt
    20881 ttacccacat cgctttgata aaagccactt ttggctaatt gccgtcgagc gacttcttcg
    20941 tttccagtca atgtgatggc gtaagccagc aaacggctat cgggatcggc aaactgcagc
    21001 tgctgcggca aataaccgat ttgcaagcta ctacctaatt tgatggtgcc accgtaaggc
    21061 ttttcctcac cgagtaataa tttgagtaag gttgattttc ctgcaccatt gtcgccgatg
    21121 atggctaaat gatctccacg atagactgca aaactgctgt ctttgaatag tacttgatca
    21181 gcgtactgct ggtgcaagtt ttgaatgatg aaaacttctt tgccagattt tccaccatta
    21241 gacaagcttt gcagccgatt tttcggggga ggtggcggtt tgacgagtgt cagtttcgcc
    21301 aggcggcgtt ccagttcttt ggcttttttg aagaaggact cattgtctcc ttcatgtgcc
    21361 cactgacgaa aacgacgaat catgactttc aaccgctgga cttctttttg ctgcagttcg
    21421 taattttttg tcagctcagc caagcgagct tctttcaaga ctcgaaaacg gctgtagttt
    21481 ccgggatact caatcaatgc accttcttcc agttcccatg tttttgtggt gacttgatcg
    21541 agaaattgcc gatcatgaga aacgaccaga accgattttt ttgtattctg taaatagttt
    21601 tccaaccagc gaatactgtt aagatctaaa tgattggtcg gttcatcgag taaaagtaaa
    21661 tcggcatcgg ctgctaaaat tttggctaaa gcgactttaa ctcgttcgcc tccgctaagt
    21721 tctgttaacc gtgcttcctg acggtgtgga atccctaagc cttgcaagat cgctgcaatc
    21781 cggtcttcta atacgtaacc gcctagttct tcaaagcgat gctgcagctc accgtaacgg
    21841 gccagacact tctctaagtt tgtatctggt tctgtcatca atgcttcgta gcgactcatt
    21901 tgctgtttga ggtcctgtat ctcagcggca gaatggagca agtagtggtt gacggtcgct
    21961 tcggaaactg taagttcttg ttcgaccgtc ccgatcttta agcctttttt gcgactgata
    22021 acaccgctgt cgacaccttc tctgccggtc aagatttgta aaaaggttga tttgccagtt
    22081 ccgttgatac cgactaaggc aattttttca ccagcttcaa tttgacagga taaatcctca
    22141 aataatggat tcccgccgat tgttttttta atagaaattg cttgtatgat catgatctct
    22201 ctccttgaga ccactcaaaa aggccatggt tatccatggc ctgtgttatt taaactcaga
    22261 ttatcatgag aaaaatacct tgagatggcc attgatttta cgtgaattca actgttttgg
    22321 acaaacttgt cccgtcaaca gcaactgtaa aaccaattgc atggctaaat gcgatatggt
    22381 atcgttggta attttcatta cttgttattt tagtcatgcg catcatagta ttttccctcc
    22441 ttcgtgtagt tgtcttttcc agtgtacctg aaagtgtcag aaagttcaac tagcgctgac
    22501 atttacttaa tatttatcga tgtgtaattc tgtcagagag ctttttttat ctgactgagg
    22561 gtaatgtctc gtggaccaca tcattgtatt ctgagctaaa ccagcgttca ggcgaaatca
    22621 tatggtgttt gataaaaaac tttgtctaca tgacttcctt ttgccatttg cttaataccg
    22681 ttgcattaaa cagctcttct ctttggaaat ctcaccaaca tcgcgttcag gagtatcgtt
    22741 gtggttgcaa tggcaagcgg tagcctttca tcaattcttg aatgatttac cgaatgtagc
    22801 acttgccttc tcgcggtacc cgaacccgaa ataaaatttt agtttggcga taaaatcggt
    22861 atccgccatc gatacttcat attttttcgt atcagattca tcggaaactt ccacattgac
    22921 gaaccaataa gctttggcat gcttaggtcg ttttaaacca caccagggcg actaatcgcc
    22981 ctggtgttca ctttttttta ttttacttga actcattgac tgttttccct ttattttaaa
    23041 cattataaag accgatgagt gcttgccgcg tcttttgttt ttatcacggt tttttgtgca
    23101 aaaaaacctg ccgatagttg gcaggtcgtc tacgtgttaa gctttggtgt ggtctttagc
    23161 tggcaattgg ttttctaaat cagttcggac aacaagcaca tcgcaagctg cgtttctaat
    23221 aacatattca gaaacagaac caataaataa acgttctaca gcattcaatc ctgtggctcc
    23281 taacataatc aaatctactt gattatcttc tgggatttct ctggcaataa tttgttttgg
    23341 tgagccatat tctacgacag aggttacatt attcaagcca gctttcttcg cattgctttc
    23401 ataatctgcc aatgtttgct ttgccatttc agtagcttgt tctgcaagca ttccatcaaa
    23461 ggatgaaacg gtttggaaag aacgtgtgtc aatgacatgt gctaatagaa gttctccgtt
    23521 atttcggata gctacattga ccgccttttt aaaagctaat tcagcttcat cagaaccatc
    23581 tacggcaacc atgatttttt tgtattgttg taacataagc attcactcct caagtcaatc
    23641 gtattgtact cttttattgt agaacacttt agggaaaaag agaattattt actgctttgg
    23701 ttcagcaatt ttttttgcaa atcggatact gaaggtggca gaaacaataa ttacgatcag
    23761 taaaaagacc aaggcaataa agcgttgatt gaaaaaagct gttaaaatac ccattacgcc
    23821 taaaacagca tatgcagctc cgtaaaattg taggaatcct tgatttttat catttttgtt
    23881 gatcaataca aaaaagatat cctgtttatt aaataaataa tagctaacga ataataatac
    23941 taaagtggcg ataatcatta aaatcctaat catgaacaaa tcctccaaaa taagcaacaa
    24001 atagaaaaag caatttgcca agagcaaatt gctttattct ttaaaagatg gtaatctccg
    24061 ttaatgccgt tgtttgtcct gtagtattga tcccgcgaat tcaagcaggt gggtcccatg
    24121 gtcgtagctt cgaactacca aatgataagg catgttacca gcagtgacac aagacgggtc
    24181 ccaagatatc aataaaaatg ttcggtcaac acacaaagag gacaattcga acatcacaga
    24241 tttcccacct ctatagtagc atatgaattt aaaaagagca atgagagagc tcgcagaaac
    24301 agaaccgttt tcttgtctag ttttttggag gatgcttttt ccactctttt atacggtaat
    24361 actgttgacc cagagcctgt tcatatttcc ctgtatcttt tggctgataa tactgagcat
    24421 tcttcagttt atctggtaaa tattgctgat tgacccaagc ctgatcaaaa tgatgaggat
    24481 actgataacc cacgccacgg ttgagtgatt tcgcaccttt gtaatggcta tctctcaaat
    24541 gatcaggaac atctccagct ttgccctctc gaatatcagc aagtgctgca tctaatgcca
    24601 tgtaggcaga attggatttt ggcgacaagc aaagatcgac gacgacgtct gctagtggga
    24661 tacgtgcctc aggcaatccc aatttttcag cagctaatac agcgttcact gtacgagccg
    24721 cagcagcagg attacctaaa cctatatctt cataaccgat aaccatcaga cgccggcaaa
    24781 tacttgccag atcacctgcc tcaactaaac gggcgagata atgaagtgca gcatccacat
    24841 cacttccacg aatcgatttt tgaaaagccg aaataacgtc ataatgagca tctccatttt
    24901 tgtcatgggt aagagctttt ctttggacac attcttcaat gatcgacagt gtaagatgaa
    24961 tccttccttc tttatctgaa ggagtcgata gtgtagccag ttccagccca ttcaatgcac
    25021 tccgcaaatc gccgttcgtt gcacgcgaaa ggtgaagaag agcatcttcg tctaactgga
    25081 ttgcttgttg tccaaggccg cgttctttat cccttaaagc atgttcgact gccaactgga
    25141 tgtcttgttc tgttaatggt ttgacttcaa agatctgtgt acggctgcgg atcgctggat
    25201 tgatggtaat ataaggattc tcagtagtcg ctccgatcaa gatgattcgg ccactttcta
    25261 aatgaggaag cagaaaatcc tgttttgttt tatctaatcg gtgtacttca tccaataaga
    25321 ggatcactgt accactcatt tttgcttctt ccgctacaac ttgtagatcc ttttttgaat
    25381 ctgtagcggc gtttagcatg cgaaatgcat aattagtaga gccggcgatc gcacttgcga
    25441 tactcgtttt tccagtacca ggaggtccat atagaatcat ggaagagagc atgcgagcat
    25501 cgaccatgcg gcgtatgatt tttccttccc cgactagatg ctgctgacca acgacttcat
    25561 cgatcgttcg aggtctcatg cgataagcca atggctgttg cataggattt tcctccttct
    25621 tgtttttacg tcccttaatt atatcatatt tagcaagatg cctagaaaga acagccttct
    25681 gttgttttgt tttcatagct tatccaacta atattgtgta tgataaagta ggtgagaaaa
    25741 atgacggata aacaaaagat agatgaattt ttttggaata aaacgacgcc agaagtggca
    25801 cgggatttat tggggatgta tttggagcat gacacaccag aaggaagatt agctggatat
    25861 attgtagacg cggaagcata cctaggtcca gaagacgagg ccgcccatag ctatggttta
    25921 agaagaacac cgcgtgtgcg ggcaatgtat gaaaaaccag gtacgatcta cctttacacg
    25981 atgcatactc atcggatttt gaacatcatt actcagccag aagggattcc tcaaggagta
    26041 atgatacgtg caatcgaacc cgccgcaatg attgatcaaa tgagtaaaaa tcgtggcgga
    26101 aaaactggtc cagatatcag caacggtccg ggaaaattag tggaagcatt agcaattcct
    26161 caagaacttt atggtcagtc tatagcagat agttcgttac gcttagtttt tgagaaaaaa
    26221 aagacaccaa aaaagatcaa tgctttgcct agaatcggta tcccaaacaa aggtgtatgg
    26281 acagaaaagc cgttgcgttt tgttgtgtcg ggaaatcctt atatctcttt acaaagaaaa
    26341 aaccaaatcg agaaagattg gggttggagg aaagaaaatg aaaaagaagg atctattaac
    26401 atatttagat gaaataattg aaaaacaagt gacagactat gatgtagctt tagattggga
    26461 tacaaaaaat catacgatcg agatcgttgt tcgcttgttt gcagaaaata aagcacaatt
    26521 tgagattgat gatgcagaag ggatcgtttc gcaagaacca attatcgaat ttgaagacgg
    26581 cgtacttctt tttaatcctc agaaatctgt ttttgatgaa cgcaattatc tagcagtaat
    26641 cccttatgaa ggaaaaaaag ggttggcaaa atcagtagct gattctttag tggaatatct
    26701 aaatgaagta ttagctcaag ggcaaagcga tttgcttgat tttttggatg aagacgatga
    26761 cgaagctgta ttcgagcttc actgggataa tcagcaacta gcagaattag tagaagtaaa
    26821 acagcaaaat gaagcagata cttatttacc gtacccaagt tattaaggag tgacagatat
    26881 gaaatggaat gaagtaaaag tagaaactga aagtgaagca gtcgaagcag ttgctaatat
    26941 tttgatggag gcaggggcga gtggtgtcgc gattgaagat tctttagatg ttgaacattt
    27001 tcagtcggat caatatggtg agattttaga taaaaatgat tttacatcgc tgaaagaagg
    27061 cgcactagtc atggcttatt ttccggaaac gactttctta ccggaaatct tgccttttat
    27121 caaagatcgt atcacacaat tacctgagtt tggtctagct attggaaaaa ataaagtaac
    27181 agttagtgaa gtagaggaaa gcaactgggc aacagcctgg aagaaatact atcatccggt
    27241 gcgtattacg cgaatgctga cggttgcgcc aagctgggaa aactaccaga cagaagatcc
    27301 tctagaaaaa attatcacac tagatcctgg tatggcattt ggcacaggca ctcatcccac
    27361 gacttccttg acattgacag cactagaaac agtcttgcgt ggtggtgaaa cattgctaga
    27421 tgtgggaact gggtcgggcg tgctaagtat tgcagcaaaa tatcttggtg caaaagatgt
    27481 ctatgccttt gatttagacg aagtagccgt tcgttcggcg aaagaaaata tggatatgaa
    27541 tgaaatagcc aaagatgtcc atgtatcagc taacgatctt ttgacaggaa tcgagattga
    27601 aagtgatgta atcgttgcga acattttagc agatatcatc ttattgatga tcccagatgc
    27661 ttggcgttta ctgaagcaaa caggcacgct gatcgtttca ggaatcattg aagagaagaa
    27721 gcaaatggtt ctggatgcca tgttagaaca agggtttatc gtggatcaaa ttttccagca
    27781 aaaagactgg tatgcaatta ttttgaaaaa accagaggtg gaatagatgc agcggtattt
    27841 tttaaaagaa tcatatgaat caaaagacta ttttgtttta gaaggggaaa actatcacca
    27901 cattgtacgg gttatgagga tggaacccga ggatgaagtt tttttggcat tcaatgatca
    27961 aatagccatt gttgcagaaa tcacggagat tactgaagag gctgtttatc tgaaagaaat
    28021 taaaaaagaa gaaaatcaaa aggaactgcc aagttcgatt accatagcga gtggctatcc
    28081 taaaggggaa aagctggatt ggatcgttca gaaatccact gaattaggag cgcaagcttt
    28141 tcttggtttc ccggcatcag cctctgtcgt taaatgggat gcaaaaaaac gtatgaaacg
    28201 acaacaacgg cttgaaaaga ttgctcaaga ggcagcggaa caatcccatc ggcagatcgt
    28261 gccatcagta gagttattgg aaacgaaaca agatttattt gagcggctag atgattatga
    28321 cgtggtcttg gttgcttatg aggaatcagc aaagcaaggc gaaaaatcaa atgcagccaa
    28381 aattcttagt gaaacaaaac cagatagtcg aatcttagct gtctttggac cagaaggtgg
    28441 attatcccgc gatgaagtcg attattttgt agaacacaaa gggatacttt gtggattagg
    28501 accaagaatc cttcgtacag aaacagcacc tctttattta ttaagtgctg ctagctatca
    28561 atgggaatta atgaatcctt ctttcagcta acgttcttca ttaataaaag agtatcgaca
    28621 gaaagaattg aaaaaatagt caacaatttg tataatagaa acaattattt atacaatgga
    28681 tacgaaaaat cgtttgaaaa agcttttaaa aagcgaacat ttccgtaaaa aaacgtataa
    28741 ctgcacggat tttgggacaa ttatcaaaca gatatttgtc ccgattcccg ttttaccgaa
    28801 aaaagaaagg gggatcgatt cctgtgacaa aagaagaaat tttaacggga cccggtgtca
    28861 taaagctcgt tagtcaatac atgggtcccg aacatgtagc atttgttgag aaagcatgcg
    28921 aatacgcgac tgctgcacat gacggacagt ttagaaaatc aggcgaaccc tacattattc
    28981 atcctatcca agtcgcaggt atattagcag atttaaaaat ggatccccat acagtggcta
    29041 caggcttctt acatgatgtt gttgaagata cagaaatcac tttagaagat ctgagagaag
    29101 aatttggcga tgacgttgct atgttagtgg acggcgtaac caaattaggg aaaataaaat
    29161 ataaatccca cgaagagcag ctagcagaaa accaccgaaa gatgctgcta gcaatggctc
    29221 aagatttacg agttatcatg gtcaaattag ctgatagact acataacatg cgtacgttga
    29281 agcacctgcg agaagataag caaagaagga tcgctcagga aactttagaa atctatgcgc
    29341 ctcttgcaca tcgtctaggg atcagccgga tcaaatggga attagaagat acagcacttc
    29401 gttatctaaa tccaaaacag tattaccgta tcgtccactt gatgcagacg aagagagaag
    29461 aacgtgaaaa atacgtaagt ggtactgttg aagatattcg aatagcgaca gaagagctgg
    29521 ggatttttgc agaaatctat ggacggccaa aacacattta ttcaatctat cgtaaaatga
    29581 aagatcagaa aaagcagttc aacgaaattt atgacttgct agctattcga gtgatcgtag
    29641 attcaatcaa ggattgttat gctgtattag gagcaatcca cacaaaatgg aaaccaatgc
    29701 ccggcagatt taaggattat attgccatgc caaaagctaa tatgtaccaa tctttgcata
    29761 ctaccgtgat cggtccggca ggaaatccgg tagaaattca aatccgaaca caggaaatgc
    29821 atgaaatcgc tgaattcggg gttgctgcac actgggccta taaagaagga aaaaatgaaa
    29881 aagtagaacc agatggtatg acgaaacaat taagctggtt ccatgagata ctcgaacttc
    29941 aagacgaaag ctatgatgct tctgaattta tggaaggcgt aaaaggagat atctttagtg
    30001 ataaagtcta cgtcttcaca ccaaaaggag acgttactga gttaccaaaa ggatccggac
    30061 cattggactt tgcatacagt atccatacag atatcggtaa caaaaccact ggtgcaaaag
    30121 taaatggcaa aatggtgcag cttgattaca aattgaaaaa cggagatatc attgagatca
    30181 tgacttctcc aaattcattt ggcccaagtc gcgactggtt gaaattagtt gctactagca
    30241 aagcaagaaa taagatcaaa cgtttcttca aagcccaaga tcgagaagaa aatgtgatca
    30301 aaggccacga atccgtggtc aaatgtatta cagatctagg atttacgcct aaagatattt
    30361 tgacgaagaa caaactgcaa gaagcactcg atcgttttaa ttatcaaaca gaagatgatc
    30421 tctatgcagc tgtagggtat ggagaagtta gccccttgac gatggccaat cgtctgactg
    30481 aaaaagaacg taaagaacaa aaaatcgagc agcaaaagca agaagcagaa gaaatcatga
    30541 atcagccgaa aaaagaacct gacaaaatga aagtacgtca tgaaggtggc gttgtcattc
    30601 aaggggtaga aaacttacta attcgtatca gtcgctgctg taatccgatt cctggtgatg
    30661 atatcgttgg ttatatcact aaaggcagag ggatatccat tcatcgtcga gattgtccga
    30721 atgttcagcc tgacaaacca aatgtagcag aacgtttgat tgaagtcgaa tgggaagata
    30781 catcgaatac acgaaaagag tatgatgcag atttggaaat ttacggctat aatcgttcag
    30841 gcttattgaa tgatgtactt caaacagtca atgcgctaac gaaaaatctc aacagcgttg
    30901 aagcacggac gaataaagat aaaatggcga cgatccattt gacggttggt atccagaatt
    30961 tatccaacct aaagagtatc gtggataaaa tcaaagcagt acctgatgtc tacagtgtac
    31021 gccggacgaa tggataggag gacagatatg agagcagttg ttcaacgggt aagtaaagct
    31081 gctgtgtcag tcgatcagca agaaattgga aaaattggct acggcctact tgttttatta
    31141 ggcattcatg aaaccgatac gcaaaaagat gtggactatc tggtaaaaaa aattgcacaa
    31201 atgcgtattt ttgaagatga acaaggaaaa atgaatatta gtgtagaaga tgtgggagga
    31261 caaattttaa gtatctctca attcacactg tttgcggata cgaaaaaagg aaatcgacca
    31321 agctttgtgg aagccgcacg accagaaaca gctattcctt tatacgaatc attcaatgaa
    31381 ggaatcagaa gtcgcggcat cacagtcgct accggacaat tcggagcaga tatgtcggtt
    31441 tctctcgtca atgatggacc tgtaacgatc attattgatt ctaaaaacaa ataaagtaat
    31501 ctggttttca ctagtaaaaa ctatgaaaca cgtcgcttaa gttagactga taaactctaa
    31561 cttaagtggc ttttttgtct gcaaaaagcg cttcttttgt cagcagtcgg aaaaaaagtt
    31621 aagcttaaat tatacaagtt attttttata ggaggttgct gaagatgaaa gaagtaccag
    31681 tagtagatat caaaatcaca gatttaaaaa aattgtatca aaaaggatac aacatgttgg
    31741 aggagttacg ccatgaagcg gacgctcccg tcgtcaaagc aaagattttc aataaagaag
    31801 ctatcacgat ctacggatca tcagcagcaa aagtatttta tgatccaagg aatttcaaac
    31861 gaaaaggtgc tatgccaaaa ttagtattga agacactttt tggtcaaggt ggagtacaga
    31921 ctttggatgg agctgctcat caccatcgga aaaatatttt tatggatcta atgacaccgg
    31981 aaagaatgga agattatcat cgtatcttag ataagaatct gacacaagca cttgaagcac
    32041 agcatggtca atttgaacta tttgatttaa gtaagatggt tttctttaca tctatttgcg
    32101 agtgggcagg tatcaattta tcagcgattt ctaaagatga ggtcgaaaaa ttagcagaat
    32161 atcaaatctc tatgatcagc ggaacgttta cttctccgat tgatcacata aaaggtgtcg
    32221 aaaatcgcaa aaaatcagaa aaatgggcgc aaggcttgat tgaagaagct cgacaaaatc
    32281 ctgtggctgg aaaagaaaat gtggcattat atgcttttgc caatgccaca gatttagatg
    32341 gtcagctttt acctctggaa gtggcagctg ttgaattatt gaacatcatc cgtccaacgg
    32401 ttgctctaac tgtgtgggcg gcattgatgg gacatgcact tttcagtaga ccagatttgt
    32461 atcagcaatt aaaaaatgac ttttcgacgt tacaagatcc gtttattcaa gaaatgagac
    32521 gctattatcc tttcttccca atgctacctg cgatttcgct gaaagaagta gaagtagatg
    32581 gttatcgtat accagaaggc agttgggtta ttttagattt atatggaaca gaccatgatg
    32641 aacgaacagt tgaagcgcca gattccttta tgatcaaacg gtatgtaggg aaagcaaaag
    32701 atatttccta taaagaagaa tatgagatga ttgcacaagg cgggggaaat tttcgacaaa
    32761 tgcatcgctg tgcaggagaa tggatcactc ttcatagtct acgtgtgttt tccgatcagc
    32821 tagtaaataa gtttgagttc tctgtacctg aacaagattg gacgattcca ttcaatcagt
    32881 ttccgactta tccgaatagc cgagcgctac tttacaagaa ttagattagc ataaacttat
    32941 cgaaaaaaag gaagatcaaa cagtgctgat cttccttttt tcatattact ataaattgat
    33001 aaggtcaatt gatcaaaatc cacgaaatgc ttcatcaatg gtttgataat cttcattgga
    33061 taaatgcaaa tctaaagctt ggacattgtc agatacttgc tctggacgct tagctccagg
    33121 tataactaca gacactcgtg gattcttcat gtaccaagct aaaacaagtt gcgcaggagt
    33181 ggcttggtat cttttagcaa tggggcgaag tacatcgaca gcggttaaaa tttctctgaa
    33241 acgttctcct tggaaatctg gatttctttt tcgaggatct ccttcaccaa attgtttttc
    33301 ctcaccaagt tcatattttc ctgtcagcaa accggaagca agaggaaaat agggaacaaa
    33361 actgattttg tttttttcaa gataagggaa taattctttt tcagcctgcc gatggatcaa
    33421 actgtattta tcttctacta catcgacata tccatctgca ttcgcttctt tcaattgttc
    33481 taacgtgaag tttgacacac cgactgctcg gatttttcct gcttctttta attcgtgcaa
    33541 tgtagcaact gattcatttt ttggtgtgct ttcatccgga aagtggatat aaaaaatgtc
    33601 gatgtaatca gtttgcagac gttttagtgc atcttccacc gattgtttga gaaactcagg
    33661 agaattatca aatgttcgcc ctttatttgg tacatgagca gcttttgtag cgatgacgac
    33721 acgtgagcga tcatattctt tttccttcag tacctcgcca atcagttctt cggaacggcc
    33781 gtttccgtaa gcataagcgg tatcgatcaa attgatcccg ctattcaaag ctgtacgaac
    33841 tacttgtttt cctgtctcat cttccaaacc gtcaaaaaga ttatgtcctc caacggcatt
    33901 tgctcctaat ccaagctgtt ctgcgtagac ttgtgaatgt ccgattctta tttctgccat
    33961 agctgattcc tcctttttgt ctagtctaaa acaaaatacc aaaaagctga actaaaacgt
    34021 ttttgtgagg ggaaatagtc agatatctct tgactttgag actggttttt agtaggttat
    34081 aagtaatatc gaatgtcaat gaaagagcga gtaagttttt gaaactttac aagagaggat
    34141 catcgaggct gaaaatgatc taaagtttca ttagcctaaa gacactctgg agatggacga
    34201 gtgaaaagcc gtcccgcgta cgagcgttat tcgtaagagg aaagagaaat ctttcaaaat
    34261 taggtggtac cgtgtatgaa tttcattcac ccttgttcat gatgaacaag ggtgattttt
    34321 ttatttacat agctaagaaa gggagagaaa actatgagtt tccaaagacc aaaaggaaca
    34381 aatgatattt tgccaggcga atcagaaaaa tggcagtttg tcgaagaaac agcacgttta
    34441 ttattcagag attatcagta taatgaaatg cgtacaccga tttttgaaca ctatgaagtg
    34501 atatcccgaa gtgtagggga tacaacggat atcgtttcaa aagagatgta tgatttttat
    34561 gacaaagggg aacgccatgt gacgcttcga ccagaaggta cagcaccaat cgttcgttct
    34621 tttgttgagc ataaattgtt cggcccagag tatgcgaaac cgttcaaaac tttttatatg
    34681 ggaccaatgt ttcgctacga acgccctcaa aaaggacggt tacgtcaatt ccatcaaatc
    34741 ggagtagaag catttggatc agaaaaccct gcaactgatg tggagagcat ggtgatggca
    34801 cttgatttct tcaaacagtt ggggatcacg caaatccggc tagtcatcaa ttcattaggt
    34861 gataaagaaa cgcgtcaagc ctatcgtcaa gcattgatcg actatctttt accttttgaa
    34921 tcagagctga gtgaagactc taaacgccgc cttcatgaaa atccattacg cgtattggac
    34981 agtaaagaca aacgagatca aaaatttgtg gcagaagctc catctatctt agattatcta
    35041 agcgagcctg ctcaaaaaca ttttgatacc gttgtttcca tgctggatgc attggctgtt
    35101 ccttatgaaa tcgacagtaa tatggttcgc ggactagatt attacacaca tacgattttt
    35161 gaaatcatga gtgacgcacc aaaaatgggg gcacaagcaa cgatttgcgc aggcggacgt
    35221 tatgacaatc tagtggaaga actaggtggt ccttcaacac ctggcttcgg atttgcaatg
    35281 ggtatcgagc gattactctt gactatggag gcagaggaag tagttatccc tgcatttaat
    35341 gagttggatg cttacgtggt cgcactcgga gacgaaacaa atatcgaagc attgaaagtg
    35401 gtccaagcga ttcgaaactt tggtttttca gctgaccgcg acttcatgaa ccgtaaagca
    35461 aaagcgcaat ttaagacagc agataaagcg aatgccaaat tagtattggt gatcggtgaa
    35521 gatgagcttg ctagtcaaac gattaatgtc aaagcaatgg caagccgaaa agaaaaaagt
    35581 tttccattag aagagatata tgaacatttt gatgaagtct atgatgaaat gactttattc
    35641 gagtaggagg aaaaaaataa atggcacaaa gaacgatcta ttgcggatta gtttccgaac
    35701 aacaattaga acaaacagtt acattaaaag gatgggtaca aaaaagacga gaccttgggg
    35761 gagtaatctt tatcgaccta cgtgatcgtg aaggaatcgt tcaaatcgtc tttaatcctg
    35821 aaatcaacaa agaagcatgg gaaatcgcgg ataaatgtcg cagtgaatac gtgatcgaag
    35881 tgactgggga agttagaaga cgcggagaaa atgcagtaaa tcctaaaatg gccactggtg
    35941 aattcgaagt gattgcaagt gaaatcgtga ttttgaatca agcaaaaacc ccgccatttc
    36001 aaattgaaga tgatcaagca atcagtgacg aaatacgttt gaaatatcgt tatttggatc
    36061 tgcgtcgtcc aaaaatgact aaaaacatca aattaagaaa tgcgacaaca aaagcgattc
    36121 gtcattattt agatgataat gactttctcg atatcgaaac accttaccta gggaaatcta
    36181 caccagaagg cgcaagagac tacctagttc cttcacgtgt ccatgctgga catttctatg
    36241 cgttgccgca atctcctcaa ttgtttaaac aattgctgat gaatgctgga ttcgaccgtt
    36301 attatcaaat cgttcgttgt ttccgcgatg aagatttacg tggagaccgt cagcctgaat
    36361 ttacgcaagt agatattgaa acaacgttct taacagcaga agaaatccaa acttacacag
    36421 agggattgat tgcgaaggta atgaaagaag ttcgcggtat cgaagtcaca ttgccatttc
    36481 ctcgtatgac ctatgatgaa gcaatggctc gctacggcag cgacaaaccg gatacacgtt
    36541 ttgatatgga attgatcgat ctaagcgata cagtcaaaga agtagaattc aaagtcttcc
    36601 aaatggctct tgaaaacggc ggtgtcgtga aagcattgaa tgctaaaggc gctgcagatc
    36661 gctactcacg aaaagacatg gatcagttag gtcaatatgt aggacaattc ggagctaagg
    36721 ggcttgcatg gctgaaagta gaagaagacg gattgaaagg tccaattgct aaattcatgg
    36781 gagaagcaac agaagcaatc attaaagcaa cagatgcaaa accaggtgac ttgctgatgt
    36841 ttggcgcaga taaaagtgaa atcgtcgctg ctgcattagg tgctatccgg acacgtttag
    36901 gaaaagaatt aggactaatt gatgaatcga aattcaattt cttatgggta actgattggc
    36961 cacaatttga attttcagaa gaagaaggtc gatatgtctc tgctcaccat ccattcacga
    37021 tgccaaaaga agaagatatc ccgttattgg ctgaagaccc tgcaaaagtc tatgcagaag
    37081 cttatgatat cgtcttgaac ggttatgaac taggcggagg ttctatccgt attcatactc
    37141 gtgagcttca agaaaaagta ctagaaactc taggctttac aaaagaacaa atggaagaac
    37201 aatttggttt cttgttaaca gctttagatt acggattccc tccacatggc ggtattgcat
    37261 taggattgga ccgttttgca atgttattag ccggagaaga taatattcgt gaagtcattg
    37321 cctttcctaa aaacggaaaa gcagcagatg tgatgagtga tgcaccaagt atggtttcgc
    37381 ctttacaatt gttcgaattg aatattgatg taacgaatat agaagaatag ccgataaaaa
    37441 ggtaaaaagg atttgtcgaa tacgcttctt ttgacaacct tgatttaatt gaaaaacact
    37501 ttataatgaa gccataacat ataaagcggg gcttttccat gaaaaaaaca ttcgaaaatt
    37561 gggcttatca tgattacacg accaagcttt cggtatctat cgtttatgcg ttgatcgcat
    37621 cagtcgcatt gaatttcttt tatcagccag gacatattta ttcaagtggt attactggtc
    37681 tagctcagat tttgacgact ttgtcaaaat cactgatagg attcgaagta ccggtatcaa
    37741 caactttgta tttactgaat atacctttgt ttgtattggc gtggttaaaa atcgggaaga
    37801 agtttacaat tttcactttt atcacagtca ctttgacctc cctttgtatc catattttac
    37861 cggaaacagt cctatctacc gatccaatca tctgtgcgtt gtttgggggg gctgtgatgg
    37921 gactaggtat tgggctagcg ttgagaaacg gcctatcatc gggcggtcta gatatcgtga
    37981 gtatcacgat tcgtaaaaaa acaggaaagt cgattggttc tatttcgatc tacttcaatg
    38041 cattgatcgt ttttgttgcc ggaattctat ttggctggca atatatgttt tacagtacgt
    38101 tgtctatttt cgtcagtgga aaagtaactg atgtcgttta tactaaacaa aagaagatgc
    38161 aggtaatgat cgtaacgaaa catccagaaa aagtagtaaa tgaaatccaa aactgtatgc
    38221 gtagagggat cacgatcatc aatgaagcag aaggtgctta taagcatgat aagcagacag
    38281 tattgatgac gatcgttaca cgatacgaac tgcctctact tagagaagca atgaaagctt
    38341 ctgatccaca agcttttgta agtattgcag aaaacgtcca gatacttggt cgtttctatg
    38401 aggatggcat ttagatcgag tttgacaaaa attcatgaaa tactgctgta gacagacgtt
    38461 cgtttgtctg cagttttttt ctaccatagt agaatcagca aagaaaaact gcttttttat
    38521 ttgaaatttt cttaaagttg aaaaaaagca tgtttatttt tttaaattta tgggatgata
    38581 gaaaaaaata gtagagaaag gacacgattc tacttgctat tttcacttaa tacccgtata
    38641 ttacgaatga aaaaagttta tgaaaagttg gaatattgaa tggaaaaaca aaaaaggtta
    38701 aaaaacagcc gctacatcac tgggtttgat gggctccgtt cattagctgt ggtgggtgtt
    38761 atcttctatc accttctgcc aactagtatg aaaggcggct atttgggcgt tccgattttt
    38821 ttcgtcgtat ctggttattt gatcacggat ctgctgcggc aggaatggga acaaaatgga
    38881 aaaatcaata tttggcaatt ttatgtcaga agaatgaaga gactctatcc ggcacttgtt
    38941 tttatgcttg tgacagcttc ggcttatatc actttgtttc agcgagggct gttaaacaat
    39001 cttcgaggag ttgtggtcag cagcttactg tatgtcaaca actggtggca aatcaataac
    39061 ggtttatctt atttcgatcg atttgctaat gagtcaccgt ttacgcatat ctggtcttta
    39121 gcagtagaag gtcaaaacta tttggtttgg ccgatcttgt ttgtattgtt gatggtattt
    39181 gttcgtaaaa gaaaatggat cttttatact gtgttaggta catccttcct ctccgccatc
    39241 ttgatgatga tcttgtatac accaggaggc gatccaaccc gtgtttatta cggtaccgac
    39301 actcgtctgt tttctatatg gttgggaagt gcgcttgcgt ttgtttggcc aagtacgcgt
    39361 ttaaagaaaa acatcccgaa acaagcgaaa aatattctga atatcgctgg aggaatctcg
    39421 cttttggcac tgattctttg cttcttcttt ttagatgatc attacaattt catctattac
    39481 ggaggaatgc tcctagtgag tctgttatgc gtagtgctag ttgcagtgac agcacacccc
    39541 ggagcaagtt tgaatcgatg gctgacaaat ccagtattta cttggattgg caaacgaagc
    39601 tatggcattt atctgtatca gtttccagtt atgatttttt atgaagcaaa ggtgactgat
    39661 attgccgatc atgttttgtt gcatacttta gtggaaatca cattgattct aatcatcagt
    39721 gaactttctt atcgatatgt ggagcgtccg ctagcacgtt ttgattatgg tcgtacattc
    39781 caagtgatga aaggatggtt cagcaaaccg atcatcagta agagcaaacc atgggttgtt
    39841 ccaggcacat tacttgtagc aattgcactc gttggtttcg ttacggcacc gaaaaacgct
    39901 gtaacagccg atcaaaaaca actgcaggca aaaatcgctg aaagtaaaaa attagcagaa
    39961 gaaaccagaa acaattcaga aaacaccact agttcggttg accaagcgat ccttgataag
    40021 tacgagttgt ccaaggcaca agggaataag gcacaaaaat tagaactaac ggcatttggt
    40081 gattcagtta tgttggatgc tgcaacggat ctacaagaac tttatccaaa agtagtggta
    40141 gatggagatg tcggacgaca attatatgcc agtgtaccgt acattgaaaa attaaaggaa
    40201 caagatttgt tgaaagacac tgttctgatc ggactaggaa cgaatggctc attctcggaa
    40261 gctcagtttg atagtgtgat ggatgcaatc ggtgatcgaa aagtatactg ggtcaatgtc
    40321 cgagtaccga cgaagcgctg gcaaaatgac gtgaatgcga tgcttgaaca gatgacgaaa
    40381 aaatacgaca atatgacgct gattgattgg tacagctaca gtaatgacca tagtgactgg
    40441 ttttatgatg atcaagtcca tccaaatcct gaagggatga cacattacat tcacttagtc
    40501 agcgaagcct tgctaggagg aagttcaagt acgaacagca gtgagacaaa cgtgacagaa
    40561 tgatagctac aaaaaacttc aggcaaaaac atttgttttt gcctgaagtt tttttatatt
    40621 taccgtgttt ttaacattca tatagattca ggaagcaaaa cggttgagtg tgtctgtaaa
    40681 taacgttctt tttgttttcg ggtgagtttt ttgaatgcat cttctgcttt tgttgcttcg
    40741 cttcttgaag aaaatacttc tgcatggatc atcttaacag gtcttctttt ttgaagacga
    40801 gtatatttag caccagtacc attgttatgt tcctctaatc gtctagaaag atcggttgta
    40861 tatccaccat aaaatgtctg atctttacag aataatacgt aaaaataatg ttcatttgcc
    40921 atagagcatc tcacgaatct ctgcagtgta ttctccatcc atattgtaag tgatcaatgg
    40981 tggagcgact ttgaatccgt ctaatttgcc ttctttgatt ccttcaacta acaaaatatt
    41041 tgcctctttt ccaggttttg ggtaaacaaa ttggatcctt ttaggtgcaa ttcgatagtc
    41101 ttccatgacc tgaatgattt ctaagaaccg atcaggacga tggaccatag taaagcgtcc
    41161 atttgttttt agtagcttac ttgaaatatc gatcacttct tctaacgtcg tatgaatctc
    41221 atgacgggcg attgccaggt gtttattagg gttcgtttta tttgttggca gccctttgaa
    41281 ataaggtgga ttacagacga ctaagtcaca agaattatgt cgaacgacag ataagctgtc
    41341 tttcagatcg atggtgtgga cagtcatttg ttcatttaaa tggttaagcg cgatactacg
    41401 tcttgccatg tctgctagac gaggttgaag ttcgatagcg tcgatagccg cctgggttcg
    41461 tttacttaaa aaaagtccaa ctgcgccatt tcctgcacaa aggtcaacaa tcttcccttt
    41521 tttaggtaca cgggaaaagt gagcaagcag cacagcatct aaagaaaaag aaaacacttt
    41581 ggaactttgg ataatcttga tatcttctgc gtataattgg tcgattcgtt cgtcttcgtg
    41641 aagcatggat tccctccaaa atgaaaaaaa tagtcgtctg tattatacta tttttattaa
    41701 aaagaaccaa ggtggcttgt caaacctcta ttttttatgc atactatgac cataagaagg
    41761 gaaaggaaga gactatgttt tatcgattta tgcgcggggt agttcgagta ctattagcct
    41821 tagttaatgg aaatgcccgc tatgagaata aagaagtttt accgcaagat gagaattaca
    41881 tactagttgc tccccatcgt acttggtggg acccattata tctggcggta gctgcagcac
    41941 ccaaggaatt cagctttatg gcaaaagaag aactgttcaa aaatccagtt ctgcgattta
    42001 tattaactcg atccaatgct tttccagtta aaagagaaaa tccaggacca agcgtcatta
    42061 aaacacctgt aaagattttg aaatcgacaa atttgagttt gatcatgttt cccagtggga
    42121 caagacatgc tacagaactt aaaggcggaa tggcattgat ttccagaatg tctaaagcga
    42181 aaatcgtgcc agctgtatat caaggtccgt tgacacttgg ggatctgttc aagcgtcaga
    42241 gagtgacagt gcgtttcggc gaaccaatcg acttatcaga tatcaagaaa acggataaag
    42301 aaggtatggc ggagatcgaa cgacgaacac aagcagcttt tgacaaattg gacaaagaag
    42361 tgaaccctga tttcaaatat gaagtcaaaa aaaaataaaa tgataagttt atgcgtgggc
    42421 ggtcttttga atctactgtc ctgttcagtt cagacttttt caatgaaaac gagggagaga
    42481 cgtcaagtct gccctctttt tgcgttccaa tatgtaaaat agaagacagg tgtcatgtaa
    42541 tggatcaaag tgttataata attagaagga cattttttga ggtgattgaa tgcgtttttt
    42601 acatacagcc gattggcata tcggaaaaaa actacaaggt tatgacttga ttgaagacca
    42661 gcgtcatgta ctatcttcta tactagatat cgcgaaagaa gaaaaagtag aggcaatcgt
    42721 gattgccgga gacttatatg ataggagtgt cccagctgtt gatgccgtag aattatacaa
    42781 tgaattgatg gtcgattgga atttaaagga aaagatcccg atttttgcta tatcaggaaa
    42841 tcacgatagc agtacgagac tttcagcggg tacgccatgg ttcagttatt cgaactattt
    42901 tttgcgaaca cagctagcag aagcatttga accagtcgag tttaaaaacg tacaattttt
    42961 ctttcttcct tactttgaac caattgcagc gagattatat tttgaagatg acaatatccg
    43021 aacgatcaag caagcggtag ccaaggtgat agataaaatg caggagctat ttgattcaga
    43081 aaaaaaacaa gtattgatta gccatttttt tgtaatggga agtgtaaaat ctgattcgga
    43141 aacgaaaatc gaagtaggcg ggttggattc gatccctggt gaactactta caccgtttga
    43201 ttatgtagca ctaggacatt tgcataatca agctgctttg agacaggata atgctcgcta
    43261 tagcggttcc ccattgaaat tctctttatc tgagatcaac caaaaaaaag gcgtatggat
    43321 cgttgacctg acaacaggcc tggagttgaa ttttagggag atcgaacccc tgcatgatat
    43381 tgaagaaata acagcgagtt tcaaagaatt gctggatcct gccttttatc agtccgtcaa
    43441 tcgggagaat tacatctata tctatctcac tgatagaacg atcatcccga atatgatgaa
    43501 ccagcttcgg caaatatatc ctcgtatttt ggggattgaa cgggcaaacg gtcgagcaga
    43561 acaaacaaag cgcaaacgaa aagtactaga aatgaaagat cctcaaaaat tagcagaagc
    43621 attttttagt gaaatgacgg aagagcagct aactgcaaac caagaaaaat ggctcggaga
    43681 aacgttgcaa gaaatcatgg aaatggaatg atcaagaatg aaaccgaaaa aattacaact
    43741 taaaaatttt ggtcctttta tcaatgagac cgtagatttt tcacgtttga cagaagcacc
    43801 gctttttttg atcagcggga agacaggagc tggcaaaacg acgatttttg atgggatcac
    43861 cttcgcactt tttggtgaaa cttccggaag acttcgctct ggaaaagaga tgcgttcttt
    43921 gtttgctaca ccagacgaag aaacaagcgt gactttttct tttgaacatc agcagatgag
    43981 ctatgaaatc gaacgaaaac cagaacaagt tttagcaaaa cgaaaaggaa acggaacaaa
    44041 aaaacaggca gccaaagtaa cgttgaccat ttttgatggg aaaggaaaag aaataaaaca
    44101 aatcacaaaa cgtacagaag cagatcagtt gatcagagaa ctgatgaatc tcgatgcgaa
    44161 acagttttct caaatcgttt tactacccca aggagaattc cgtaattttt tggtttcatc
    44221 aagcagtgat aaagagactg ttttacgtca tttattcggt actcaatttt tccaacagtt
    44281 caatgagcga ttaaaagaaa aagcaaaaca acagcaacaa aaattggatc atttagagca
    44341 agagctgcat cttctgcaaa aaaggtttgt tttagtcaaa gaagaggaac cagcagcagg
    44401 gtttgaagcc gttttagccc aatggacaaa ccatcaggca atactcgaaa aacaaatcac
    44461 ggaagaaaaa gaagctctaa cggaacttca ggaaaaacaa aagcaactag aaaattccta
    44521 ctatcgtttt gaaagcttga aaaaaagtta tgaagaaaaa acgatattaa taaacaagca
    44581 aaaggaatta gctgctcaaa aagacgaaat agaggaaaag aaacgatgga ttgggtatta
    44641 tgaatttacg caacgattag tcgaaccaat ccagcgccta caagaaataa aagttgaaaa
    44701 aatagaactg tcacaaaaaa gtagtgagca aacaaagaaa ctagcgatga ctacgcagaa
    44761 ttatgaagag tggcaaaaaa atgaagcaaa ccgcgtacgc cgacaaacaa aaatcaaaga
    44821 agctgctcaa aaactgcaaa acaaacagat gatggtgcca attgtcgaat ccttacgtag
    44881 taaaaaacaa gacgcagaac aattaacaat ttcattggat aaagaagaat tgaatgccga
    44941 gaagctgtat caaaatctcg ctatctatca ggaaagaact cgaattcttc aagaaaattt
    45001 actggatcag gaaaaactgc aagaagatcg tcttgctttt ggacagttaa aatatctaga
    45061 tgaaaagcag caagaaatag aaaaacaaaa agcaaaagac actgggtcgc tcgcacagat
    45121 aagagaagaa ctcaaaaaag caactgaaga aataacaaaa aatagtcagc tattgaaaga
    45181 acagacagaa caagcagaac agctgaaaag ccaatgggca aagcagcaaa ttgctcgtct
    45241 tcagcttttt ttgcgtccag gggagccatg tcttgtttgt ggatcaacgg aacaccccat
    45301 acaacaatta aaacacgaac agccaaataa ggaagatatc ttaaaagtag aacaacaact
    45361 ggcagaagct gagtctgctg tagaaagaac tcagcaaatc attgccaaaa gtgaagcaca
    45421 tgaaaataat cttcaacaac aattatcaga tttagaacaa aaaatcatgg caacgaatat
    45481 acaattggct gaacaagaag aacacttgca acaggctttg aaaaaggcat ttcctgagat
    45541 aacagaacaa aaaacaaaaa caactcttgt agtaatcgaa caatctttag atagacgaaa
    45601 aagcaagatg gccaatgcaa gaatagagct ggaagaaata agacaacaag cagaaaagct
    45661 cgaacaacaa ttagctgtct acaaacagca gcagacagaa aaaaataatc agctccagca
    45721 actgcaagga gaaatcaaaa gtttagaaca acaattgcaa gatacggata gtaactgttt
    45781 agaagaggag atacgacgac ttgaaaaaca gttagaagaa gactcgaatc aactagctgt
    45841 tgagcagaaa gcaggagtgc aattgttgca agatcttcat acgcttcgga cacagcttga
    45901 tcttcaacat gaacaaatag aaaaaataga tgaaaaaata gaaaaggaac aagaaagaat
    45961 agctaaaaag ttagaaagcc agtcgtattt ttcaacaatt gaagaaatct gtgcatttgc
    46021 acaacagcaa gcacaatata aagaaaacca acgtacagtt acttcttatc atgaagagca
    46081 attagtgaca gctgaccggc tgcgtcaatt agaaaatatc cagctaactg agacattccc
    46141 agatacagaa gagatcagcc aacagttaga agaaataaga cgtatgatcg aaataaagca
    46201 agaaaagata tacggtttac aagaacaaaa aattagtaat caaaaaatct acgatgaatg
    46261 tctttcgatt tatcacacaa gccagacgaa attagatgag cttacgcaat tacagcagct
    46321 ttcgcaaaca ttcaacggag aaaatcctaa gaagactagt ttggaacggt atgtgcttca
    46381 ggtttatctt caagaagtgt tacaagtagc aaatgatcat ctccaacgtt taacgaaaaa
    46441 tcgttatcaa tttgagctag cggataccat tggcagttat cgagggaaaa ccggattaga
    46501 aatcaatatc tatgatgatc aggcaggtat gtctcgaagt gcgcataccc tgtctggcgg
    46561 tgagagtttt attgcagcac tttctttagc attagctctt gccgatgtga tccagacaca
    46621 agctggtggt gtagcaatcg atgcattgtt tattgatgaa ggatttgggt cattagatga
    46681 agaagctttg gaaatggcaa tggaagcttt agaaacgatc gaaagtgaag gaagaatgat
    46741 cggcatcatt agtcatgtaa gagaattgaa agaacgagta ttacaacaaa taagaattga
    46801 tacagaaggt agcggacaaa gcaaaatacg ctatcaactt ggataaaaag ggagattggt
    46861 aactatgaaa tggagtattc cagaaaaagt cattgcacgt ggacgtcaat acttgcaaga
    46921 aggccgagtg ttatcggttg tccctgacca agaagaaaac gtgtggcatg cagaagtctt
    46981 gggcagtgaa ttgtatatgg ttgatttgga tggtacagca aaagagatag attactgcca
    47041 atgtccctat tgggaggaac ataaatattg taagcataca gttgctgtgg aactttattt
    47101 acggtcgaaa aatctatctc gtatcatgaa aaaagaccag ccaatgctag aaccggtaaa
    47161 agcaacaagt gagggagaaa tcattgcaaa aggtttttct cgtctaaaaa cacaaaacaa
    47221 tcctcaagcg gtgcaacctt tgattattga ataccaagta gaaacgattg agactaatca
    47281 gtatcaccca gagctttcta ttttggcact ttacttgcgt atcggttcat tggcatcacc
    47341 gaaaaaaaca tatatcgtaa aaaacatcta tgatttttta caagcttaca cagaaaaaga
    47401 aacttacact gttaataaac agtaccagtt tcgtctagat aaacgggcgt ttcaaaaaga
    47461 agatcaaaaa atactcacgc atcttgcagg aagtgctcag acgcatcaat tattaggtac
    47521 gaacggctta caagtcaaag gaaaactaga taaaagatat ctgcttctcc cagtagaaca
    47581 agcccgcttc ctgttagagc agatgaatca gactacacgt ttgcatctgc agataaatga
    47641 acaaaacgat caaggaatac aattttccga tcaaaacaaa cctttatcat tcaaagtgga
    47701 aaaatttgga gaagattatt tgctgcaagc attgaatgat tttgattttt tctttatgca
    47761 ttatcagtgg ggagttatcc aagggacatt ttattctctg acgaaatatc aacaaacggt
    47821 ttatcaaact tggaaacaat tgattcgccg attggagaaa ccagaagtag tgttcaagaa
    47881 aaaagaatta ccgtctttgt ttaaagagat tatccctctg ctgtcagaga tcggcgacgt
    47941 agctattgcc cctgcagtag aagaggaagt agcagatatc cccgtcgaat ttattttctt
    48001 tttccgaaaa gcaaaaggaa aaatccaagc tcgagtcgat ttcgtctatg gcgaagtcat
    48061 ctattcgact gacccgaagc atgaggtctc gacagaacag gcttttcagg tgttacgtga
    48121 cacagtagaa gagaaacgag tacttgatct attcagaatg tatcgctacc agaaaaatga
    48181 aactggttat gaacgtccat tgccaagcgg ggaagaacta tatgcctttt tccgcacaga
    48241 actatctgtc ttccgtcagt ttggtgaagt cagacttgga agaaaattgc gagagttgta
    48301 tttagatgca cataaacatc aaccgaaaat cgaagtaacc gagaccggtt catggctcga
    48361 tattcgattc gatgtgacag ggatagagga gcaagaaatc gatcatgtcc tgcaaagctt
    48421 gctgagaaat gatgcttttt atacattaga aaatggtcag gttctttctt ttgattcaga
    48481 agaatttcaa caaacaagcc aagtgttgca acaattacgg gaatcgatac gtacagaaga
    48541 aggaacgatc catgtaccta aaaatcaagg attgatcatt caaaatcaat tggaaaaaag
    48601 caatgctact tttagtgaat cgttccaaac gatggtacaa gatctcattc atccagaaag
    48661 ataccaggca cagcttccca aaggattgaa tgcaacgatg agagattatc aaaagcaagg
    48721 ttttcgatgg ttgaagatgt taggtcatta tcagtttggc ggtattttag ccgatgaaat
    48781 gggacttggg aaaacactac agacaattgc ctttttactt tcggaaaaag aagaacggaa
    48841 aagcttctct gctttgatcg tagcgcctgc gagcttaatt tacaactggc aggcagaggt
    48901 gagaaaattc gccccttctt taagtatcca agtaatcaat gggaataaaa aagaacgtga
    48961 agaattactg gctaaagaca cagatatccg agtgacttct tatgccagct taagacagga
    49021 tctagctaat tatcagtcgc aaaaaattga ttatttgatt cttgatgagg cacaaatggt
    49081 caaaaatagc agtacgaaaa ctgcacaagc attgagagaa ttagctgtac ctcagcggtt
    49141 tgcactgagt gggacaccaa tcgagaataa tttagaagaa ttgtggtcat tgtttgcaac
    49201 gattatgcca ggatttttcc cgactaaaac aaaattcagg gaacttgccg cagaagaaat
    49261 tgcgcaaatg attcgaccgt ttattttacg gcgagataaa caaactgtgt tgaaagactt
    49321 gccggaaaaa acagagatga atctatattc tgcacttaca gaagaacaaa aaactgttta
    49381 tttagcttac ttgcgtcaaa tgcgtgaaga aatcacatca atggattctg aagcattcaa
    49441 gaaaaaccgg atcgggatac ttgctggtct gacaagactg cgacaaattt gctgcgatcc
    49501 tcgtctgttt attgaagatt accaaggcgg ttccggcaag ctcgaacaag taaaagatct
    49561 actggtagct gccaaagaga acaaacgaag agttttgctg ttttctcaat ttacagggat
    49621 gctgactatc ttgcaagaag agttagccga gctagggatc tcgacatttt atttaagagg
    49681 cagtacgaaa ccacaagatc ggttaagtat ggtagacgca tttaatgctg gcgaaaaaga
    49741 tgtttttctg atttccttaa aggctggggg aactggattg aatctcacgg gagcagatac
    49801 ggtcatcttg tatgaccttt ggtggaatcc tgccattgaa gaacaagctg ctggacgtgc
    49861 acatcggatc ggacaaaaaa atgtggtaga agtgtggcgt atgatctctg aaggaacgat
    49921 tgaggaacgc atggattcac tccagcaaga aaaacgcgaa ctgttccaaa aagtgatcca
    49981 aggtaatgaa gaacagttga cgaaactgac tgaagaagac atccgcatga ttttaagtgt
    50041 aggagaaatc gaggagtaag agactcgaaa aagcgttcag taatgggtaa gaaagccttt
    50101 ctaaagaagc gattgtttat ttgatttaaa aaatagcaag gaggctggga ttccatttta
    50161 tcccagcttc cttgctatca tttttttatt tctattttta aaggttatac aaagattgtc
    50221 ttcattgtct ctaacagctg atcaaaagaa gaaatctcga tatcgcaaat tggagtctgt
    50281 tcaattttgc gttctcgatg gttgaaccaa agcgcttgcc agttagcact ttttgcacct
    50341 agaacatcat tgtcgtaatt atcgccaacg tataaggtct cttctggaag catatgaaat
    50401 tctttctcag ctagctgaaa aatctctttt tctggttttt ggaaacctgt tgcttgagaa
    50461 atgagcatat tgcgggaagg aatccaattg ttcaattgaa gttgcttcag tttttttgtc
    50521 tgatggtcag tcggcccatt tgtgataatt cctaatggga cattcttttc cttcaagaaa
    50581 tcgagggttt ttttgacctc ctcatgcaag cagatattat caagttcttc ctcgtaaatt
    50641 ttttgaaata agaggccatc ttcttccgta atatgagggt agtctaaatc ttttagtgat
    50701 tggctgatcc gatgtgctcg catatattct agtgtccaat ctcctgccat tacttttggg
    50761 aagttttcat cactatgatg gcggaaccgg atatatagtg gatgcatatc tgcatctgaa
    50821 acaagcggaa cgactctttt gaccgcgttt cgaaatggct gctgctgatc atacatcgta
    50881 tcatcaacat caaacacaac tgatttaatc aaaattttac acgcctttcg gttttcattg
    50941 attttttcac aaatccttga ctattgtaac agaaaaaatt gtctaaaaaa gctgataaat
    51001 aaaggttttt cttactatat ttctatttcc ttgacagaat aaggttttta tatttcagtt
    51061 tttttcacta aaaatcgtga aac
//
LOCUS       DAXWOO010000004        49288 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.004, whole genome shotgun sequence.
ACCESSION   DAXWOO010000004 DAXWOO010000000
VERSION     DAXWOO010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.004"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            136..507
                     /locus_tag="ACGE70_000156"
     CDS             136..507
                     /locus_tag="ACGE70_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFF8691829.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(781..1473)
                     /locus_tag="ACGE70_000157"
     CDS             complement(781..1473)
                     /locus_tag="ACGE70_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFF8691830.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(1476..2261)
                     /locus_tag="ACGE70_000158"
     CDS             complement(1476..2261)
                     /locus_tag="ACGE70_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFF8691831.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            2498..3898
                     /gene="lacG"
                     /locus_tag="ACGE70_000159"
     CDS             2498..3898
                     /gene="lacG"
                     /locus_tag="ACGE70_000159"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFF8691832.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            3923..4240
                     /locus_tag="ACGE70_000160"
     CDS             3923..4240
                     /locus_tag="ACGE70_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFF8691833.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            4253..5926
                     /locus_tag="ACGE70_000161"
     CDS             4253..5926
                     /locus_tag="ACGE70_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFF8691834.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            5940..7571
                     /locus_tag="ACGE70_000162"
     CDS             5940..7571
                     /locus_tag="ACGE70_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFF8691835.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(8062..9627)
                     /gene="guaA"
                     /locus_tag="ACGE70_000163"
     CDS             complement(8062..9627)
                     /gene="guaA"
                     /locus_tag="ACGE70_000163"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFF8691836.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(9686..10609)
                     /gene="coaA"
                     /locus_tag="ACGE70_000164"
     CDS             complement(9686..10609)
                     /gene="coaA"
                     /locus_tag="ACGE70_000164"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFF8691837.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            10805..11410
                     /locus_tag="ACGE70_000165"
     CDS             10805..11410
                     /locus_tag="ACGE70_000165"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFF8691838.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            11718..12734
                     /locus_tag="ACGE70_000166"
     CDS             11718..12734
                     /locus_tag="ACGE70_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8691839.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            12875..14176
                     /locus_tag="ACGE70_000167"
     CDS             12875..14176
                     /locus_tag="ACGE70_000167"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFF8691840.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            14222..14884
                     /gene="deoC"
                     /locus_tag="ACGE70_000168"
     CDS             14222..14884
                     /gene="deoC"
                     /locus_tag="ACGE70_000168"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFF8691841.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            14905..15303
                     /locus_tag="ACGE70_000169"
     CDS             14905..15303
                     /locus_tag="ACGE70_000169"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFF8691842.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            15369..16451
                     /locus_tag="ACGE70_000170"
     CDS             15369..16451
                     /locus_tag="ACGE70_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFF8691843.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            16663..18225
                     /locus_tag="ACGE70_000171"
     CDS             16663..18225
                     /locus_tag="ACGE70_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8691844.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            18218..19351
                     /locus_tag="ACGE70_000172"
     CDS             18218..19351
                     /locus_tag="ACGE70_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8691845.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            19351..20307
                     /locus_tag="ACGE70_000173"
     CDS             19351..20307
                     /locus_tag="ACGE70_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8691846.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            20406..21572
                     /gene="deoB"
                     /locus_tag="ACGE70_000174"
     CDS             20406..21572
                     /gene="deoB"
                     /locus_tag="ACGE70_000174"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFF8691847.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            21596..22414
                     /locus_tag="ACGE70_000175"
     CDS             21596..22414
                     /locus_tag="ACGE70_000175"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFF8691848.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            22431..23138
                     /gene="deoD"
                     /locus_tag="ACGE70_000176"
     CDS             22431..23138
                     /gene="deoD"
                     /locus_tag="ACGE70_000176"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFF8691849.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(23436..24122)
                     /gene="gpmA"
                     /locus_tag="ACGE70_000177"
     CDS             complement(23436..24122)
                     /gene="gpmA"
                     /locus_tag="ACGE70_000177"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFF8691850.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(24294..24875)
                     /locus_tag="ACGE70_000178"
     CDS             complement(24294..24875)
                     /locus_tag="ACGE70_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFF8691851.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(25008..25691)
                     /gene="rpiA"
                     /locus_tag="ACGE70_000179"
     CDS             complement(25008..25691)
                     /gene="rpiA"
                     /locus_tag="ACGE70_000179"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFF8691852.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            26010..26423
                     /gene="rpsL"
                     /locus_tag="ACGE70_000180"
     CDS             26010..26423
                     /gene="rpsL"
                     /locus_tag="ACGE70_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFF8691853.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            26507..26977
                     /gene="rpsG"
                     /locus_tag="ACGE70_000181"
     CDS             26507..26977
                     /gene="rpsG"
                     /locus_tag="ACGE70_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFF8691854.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            27071..29158
                     /gene="fusA"
                     /locus_tag="ACGE70_000182"
     CDS             27071..29158
                     /gene="fusA"
                     /locus_tag="ACGE70_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFF8691855.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            29330..30517
                     /gene="tuf"
                     /locus_tag="ACGE70_000183"
     CDS             29330..30517
                     /gene="tuf"
                     /locus_tag="ACGE70_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFF8691856.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            30924..31232
                     /gene="rpsJ"
                     /locus_tag="ACGE70_000184"
     CDS             30924..31232
                     /gene="rpsJ"
                     /locus_tag="ACGE70_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFF8691857.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            31263..31892
                     /gene="rplC"
                     /locus_tag="ACGE70_000185"
     CDS             31263..31892
                     /gene="rplC"
                     /locus_tag="ACGE70_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFF8691858.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            31920..32543
                     /gene="rplD"
                     /locus_tag="ACGE70_000186"
     CDS             31920..32543
                     /gene="rplD"
                     /locus_tag="ACGE70_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFF8691859.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            32543..32833
                     /gene="rplW"
                     /locus_tag="ACGE70_000187"
     CDS             32543..32833
                     /gene="rplW"
                     /locus_tag="ACGE70_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFF8691860.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            32872..33705
                     /gene="rplB"
                     /locus_tag="ACGE70_000188"
     CDS             32872..33705
                     /gene="rplB"
                     /locus_tag="ACGE70_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFF8691861.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            33748..34026
                     /gene="rpsS"
                     /locus_tag="ACGE70_000189"
     CDS             33748..34026
                     /gene="rpsS"
                     /locus_tag="ACGE70_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFF8691862.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            34049..34396
                     /gene="rplV"
                     /locus_tag="ACGE70_000190"
     CDS             34049..34396
                     /gene="rplV"
                     /locus_tag="ACGE70_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFF8691863.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            34410..35066
                     /gene="rpsC"
                     /locus_tag="ACGE70_000191"
     CDS             34410..35066
                     /gene="rpsC"
                     /locus_tag="ACGE70_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFF8691864.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            35069..35503
                     /gene="rplP"
                     /locus_tag="ACGE70_000192"
     CDS             35069..35503
                     /gene="rplP"
                     /locus_tag="ACGE70_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFF8691865.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            35493..35681
                     /gene="rpmC"
                     /locus_tag="ACGE70_000193"
     CDS             35493..35681
                     /gene="rpmC"
                     /locus_tag="ACGE70_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFF8691866.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            35706..35972
                     /gene="rpsQ"
                     /locus_tag="ACGE70_000194"
     CDS             35706..35972
                     /gene="rpsQ"
                     /locus_tag="ACGE70_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFF8691867.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            36030..36398
                     /gene="rplN"
                     /locus_tag="ACGE70_000195"
     CDS             36030..36398
                     /gene="rplN"
                     /locus_tag="ACGE70_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFF8691868.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            36435..36743
                     /gene="rplX"
                     /locus_tag="ACGE70_000196"
     CDS             36435..36743
                     /gene="rplX"
                     /locus_tag="ACGE70_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFF8691869.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            36771..37310
                     /gene="rplE"
                     /locus_tag="ACGE70_000197"
     CDS             36771..37310
                     /gene="rplE"
                     /locus_tag="ACGE70_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFF8691870.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            37329..37514
                     /locus_tag="ACGE70_000198"
     CDS             37329..37514
                     /locus_tag="ACGE70_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFF8691871.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            37550..37948
                     /gene="rpsH"
                     /locus_tag="ACGE70_000199"
     CDS             37550..37948
                     /gene="rpsH"
                     /locus_tag="ACGE70_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFF8691872.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            37980..38516
                     /gene="rplF"
                     /locus_tag="ACGE70_000200"
     CDS             37980..38516
                     /gene="rplF"
                     /locus_tag="ACGE70_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFF8691873.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            38677..39033
                     /gene="rplR"
                     /locus_tag="ACGE70_000201"
     CDS             38677..39033
                     /gene="rplR"
                     /locus_tag="ACGE70_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFF8691874.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            39054..39554
                     /gene="rpsE"
                     /locus_tag="ACGE70_000202"
     CDS             39054..39554
                     /gene="rpsE"
                     /locus_tag="ACGE70_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFF8691875.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            39569..39748
                     /gene="rpmD"
                     /locus_tag="ACGE70_000203"
     CDS             39569..39748
                     /gene="rpmD"
                     /locus_tag="ACGE70_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFF8691876.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            39792..40232
                     /gene="rplO"
                     /locus_tag="ACGE70_000204"
     CDS             39792..40232
                     /gene="rplO"
                     /locus_tag="ACGE70_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFF8691877.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            40232..41527
                     /gene="secY"
                     /locus_tag="ACGE70_000205"
     CDS             40232..41527
                     /gene="secY"
                     /locus_tag="ACGE70_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFF8691878.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            41589..42236
                     /locus_tag="ACGE70_000206"
     CDS             41589..42236
                     /locus_tag="ACGE70_000206"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFF8691879.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            42427..42645
                     /gene="infA"
                     /locus_tag="ACGE70_000207"
     CDS             42427..42645
                     /gene="infA"
                     /locus_tag="ACGE70_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFF8691880.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            42678..42794
                     /gene="rpmJ"
                     /locus_tag="ACGE70_000208"
     CDS             42678..42794
                     /gene="rpmJ"
                     /locus_tag="ACGE70_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFF8691881.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            42812..43177
                     /gene="rpsM"
                     /locus_tag="ACGE70_000209"
     CDS             42812..43177
                     /gene="rpsM"
                     /locus_tag="ACGE70_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFF8691882.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            43205..43594
                     /gene="rpsK"
                     /locus_tag="ACGE70_000210"
     CDS             43205..43594
                     /gene="rpsK"
                     /locus_tag="ACGE70_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFF8691883.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            43674..44612
                     /locus_tag="ACGE70_000211"
     CDS             43674..44612
                     /locus_tag="ACGE70_000211"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFF8691884.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            44640..45020
                     /gene="rplQ"
                     /locus_tag="ACGE70_000212"
     CDS             44640..45020
                     /gene="rplQ"
                     /locus_tag="ACGE70_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFF8691885.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            45292..46131
                     /locus_tag="ACGE70_000213"
     CDS             45292..46131
                     /locus_tag="ACGE70_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFF8691886.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            46107..46976
                     /locus_tag="ACGE70_000214"
     CDS             46107..46976
                     /locus_tag="ACGE70_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFF8691887.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            46966..47763
                     /locus_tag="ACGE70_000215"
     CDS             46966..47763
                     /locus_tag="ACGE70_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFF8691888.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            47966..48712
                     /gene="truA"
                     /locus_tag="ACGE70_000216"
     CDS             47966..48712
                     /gene="truA"
                     /locus_tag="ACGE70_000216"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFF8691889.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(49041..>49288)
                     /locus_tag="ACGE70_000217"
     CDS             complement(49041..>49288)
                     /locus_tag="ACGE70_000217"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8691890.1"
                     /translation="KMTNLFRLSSLRAIKTTFHRFLVTEKIGNKKMIHLSTPRPQVTI
                     FLKDEKLFFEIVTCDSELNDRLTTSFLNGVQSADLRY"
BASE COUNT        15603 a         8996 c        10814 g        13875 t
ORIGIN      
        1 actcagagaa cgggttggtt tttaatagat ttaataagta ctgataatga aatttctcct
       61 ggttgggaaa acgaagttgg ttaccctgta gaagaatttt ttcaagatta ctttgcggag
      121 tatgccgata ggataatgag agattacgta acagataaaa ctcaagaagt catcaatcaa
      181 tcccagtcat taaatgaatt atctgataag gcgaaagaag aagcagttgc cattatttct
      241 tcagggatta gtgactatat tgatatgagt ggtgaaatca attatggcga taaagacaaa
      301 attgtacgca tgataaaagc ttttatcaaa acttttgatg tgaaggcaga tcttaagttt
      361 aatgatactt acctagtagg aaaattaatt aatatccttg ggacagagaa agatacggaa
      421 acgtttattt cctctttgtc tatgttgttt acagataagc atttttcaac tcagcttggt
      481 ggagaggaac tagtggaaat cttttaacca ttacgcccag cattaataaa agtatatcaa
      541 aaaagtactc ctgatgattt ttaagattgg tttgagaaat aatgtgaaaa cactattttt
      601 aatgttaatt atagatagag atgaacgcgt catatggtta aaagaatgaa aaagtcaaag
      661 atacactgct atatttcact aaggatatag cagtgtatct ttcatgtcgt ttcactggca
      721 gttttttata aaaggaggtg tgcatttcag ttgcttttct ttggtagtta gttgaatcgt
      781 ttaagaatta acggatgtgg cagacaggtg ctctgtccaa aaaccagtaa aatctttgat
      841 ggttccatct ttatttagct gtgcctgttc aaatactgtt ttgtttttac cgaatggatc
      901 aattgcaata attgttccga tccccgcacg tttggcagca agtaatcctg agtaagcatc
      961 ttcgataaca agacaatcag ccggagctaa gctcagtttg tctgccgctt taagaaaaat
     1021 atccggttgc ggtttcccag ggaaagagcc gtcgtcatag acaatcttgt cccagtcaaa
     1081 ccagcgggct aaatcgaaaa tatcaaaata aaaagcaaca ttctctttga cagtagccgt
     1141 ggcaatcgtt aatggaatct gttggtcagt cagctggtca agagtcgctt caagcccttt
     1201 tgtcaactga agctcgtttt cattttgcaa gcaaagcata cgataatagc tttctttttc
     1261 ttctgacaat acggcgatct cagaggcggt caaatgctct gaaatgaaat gtttcaaaat
     1321 ctccgtattc gtccgaccat gaatattttt taaaatatcc tcgtctgata aaccatcttt
     1381 tgtatgatca tgtatcatat gcagccaagc agcttcatgc agatgagagt ccaaaaacat
     1441 tgttccgttg aaatcaaata taatcccttt catagttacg ctcctatagt ggtattttcg
     1501 gttgaatcag caatttgcag atcgacatat tgtgcatagt gctcttgaac ctcccgcgag
     1561 atctggcgat ccgtaatgag cagatcgaaa tcatgcaaat tataaaacac ataaaaatca
     1621 tctcgattga attttttatt atcaacgagt agatatttct tacgggaatt atttagagcg
     1681 atctgctgtg cttctccttc ttcggtactg taagtagaga tcgcttcgtt gtgaacgcca
     1741 tttgaactga taaatgcttt tgtaaacttt agtcttctta aattgtcgtt ggtgatcggt
     1801 ccgacaaatg cgccggtatt tttccggaaa tcaccgccaa tcagtagaat ttctggtcca
     1861 tctgcttgag caagaatatc aaaaactgga taattatttg taatgatccg aattttcttg
     1921 ttcagtaaat gcgtcgccaa tagttcgatt gttgtacctg gtcctaagaa aatcgtttct
     1981 ccctcgtgaa tcaaggaggc tgctgtggca gcaatttctt ttttttcttg aatctgcaga
     2041 gtggcttttt cagtgtgaga aagttcttga tctaaagtgt aggaaagact ttgagcccct
     2101 ccatggacac gcagcagctt gcccgatttt tctaattcat ctaaatctct gcgaacggtc
     2161 atatcagaaa catctaattg attgatgatt tcattaacgg taaggattcc ttttttattg
     2221 accatttcta cgatagtaag caatctttct tttttcaaca ttgtcttcac ctctaattta
     2281 aggataatct atcatataaa cggacaacag tcaatctaat tcgaacaaaa gtaaacattt
     2341 agggtggttt ttatgtttgt tacaatagat tttattgctg ttttctggtt tttatgatta
     2401 tttgtgtttg tttttgttga caaatgtttt ggatgcgttt acaatgcaat cagttagaaa
     2461 gagcgaaaac tgattataga tttgaggaga gatgtacatg cttaaattac cagaagattt
     2521 tatttttggc ggagccacag cagcctatca ggttgaagga gccactaagg aaggtggcaa
     2581 aggcgctgtt gcttgggatg actttttaga ggaacaaggg cgattcagtc ccgatccggc
     2641 aagtgatttt tatcatcaat atgcaaagga cattgaattg tgtgaacgtt ttggtgtaaa
     2701 tggtctaaga ttatccattg cttggagccg catttttcct gatggagctg ggaaaccaaa
     2761 tccagaagga atcgcttttt atcatcgtgt gttcgaagaa tgtaagaaaa gaaacgtaac
     2821 gccgtttgtg acgttgcatc attttgatac ccctaaacgt ttgtttgacc atggagattt
     2881 tttgaatcga gaaacaatag aagcatttgt ttcctatgcc attttttgtt ttcatgaatt
     2941 caaagaagtc aaggtgtgga gtacctttaa tgaaatctat ccagttgcaa ccaatcaata
     3001 tttgttaggt gttttcccac caggaatcaa atatgatttt acaaagatcg tcgcctgtct
     3061 tcataatatg atggttgctc atgcccgtgt agtgaactat ttcaaagaaa acgaacttcc
     3121 gggtgaaatt ggtgtagttc actcattaga gacgaaatat gcggcaacag acgcgccgga
     3181 agataagcat gctgcctttt tagacgatgc attgtcgatc cgtttcttat tagatgctac
     3241 ctatttaggc tattattcaa ctgaaacctt gactgctctt gatgagatat gtgaagcaaa
     3301 tcaggccagc tatcattttc ctgaagaaga tttcgttgaa ttgaaaaaag catccacgcg
     3361 taatgactac ttagggatca atcactacca atgccatttt gtcaaagctt acgatggaga
     3421 aaatgcaatc catcataacg gtacgggtga aaaagggacc tctgtttata aagtgaaggg
     3481 aatcggagaa cgtatttata aagaaggaat tcctcgaact gattgggact ggctgattta
     3541 tccagaaggt ctatatgact tgttgctgcg aattaaaagc gattacccac attacaacaa
     3601 aatctatatc actgagaacg ggatggggta taaagatcag tttgaagatg gcattattat
     3661 ggatcagccg agaatcgact atttgagagt ctatttggaa tccttaagca aggcgataac
     3721 agccggggta aatgtgaagg ggtatttttt gtggtcgctt atggatcttt tctcatggac
     3781 aaatgggtat aacaaaaggt atggcttgtt ttatgttgat tttgaaaccc agaagcgtta
     3841 cccaaaagaa tcagcttact ggtacaagtt ggtcagtgaa accaaaacga ttatttaata
     3901 agtgataatg gaggaagaaa agatgaataa agaggaatta caaatgcttg gttttgagat
     3961 cgttgcatat tcaggcgacg ctcgcagtac attattgaaa ttattgaaag aagtaagaca
     4021 aggaaacttt gaacatgttg attcggcgtt gaaagaagcc gacgaaaatt taacccttgc
     4081 acataattcg caaacaaaaa ttttagcgga agaagcatct gggaaagaga tggagatggg
     4141 attcattttt atccatgggc aggatcattt aatgaccact ttattgctga gagatctgat
     4201 tcaagacttt attgtactgt acagacagaa tcaaggatag gagaagaacg ctatggatgg
     4261 aatcattaaa caaatcgaaa aagggaaacc attttttgaa aaaatctcac gtaatagtta
     4321 tttaggagcc gttcgtgacg gatttttaac agcgatgcca gctattttat tttcaagcat
     4381 tttcattttg attgctgcgg ttcctgagat ttttggttac caatggcctg aatctgtctc
     4441 cacatggcta tggaaagtct ataattattc gatgggtgtt gtagggttac tcgtttcagc
     4501 aaccactgct cgctgcttgg ctgattcgat gaatcgtcgc ttaccgaaaa acaaagtaat
     4561 caacaccacc tctgtgatgc tggcctctgt ggtaagtttc ctgttgttaa gcgtctctcc
     4621 gattgaaggt ggattttcaa cggaatatat ggggacaaag ggactactgg cttcctttgt
     4681 cgctgcattt ttaacagtta atttatataa gttttgtgtg ctgaaagata ttaccattaa
     4741 aatgcctaaa gaagttcctg gaacgatttc gcaaaccttc cgagatattt ttcctttctc
     4801 tttttcagta tttgcggccg ttttaattga tttaggcatt cgacaaatct tccaaatctc
     4861 atttgcagaa gcaatcatta cggtgctgca gccgctattt acagcagctg acgggtactt
     4921 aggaatcgct attatctggg gagcgatggc gctgttttgg tttgtcggtg ttcatggacc
     4981 gtcgatcgtg gagccggcaa ttgcggcaat tatctatgcc aatgtggaaa ccaatctgca
     5041 attgttcaat tccggtgaac aagcttctaa agtactgacg gtgggtttag ggaattttgt
     5101 aggaactatg ggaggaactg gggcaacatt ggttgttcct tttatcttcc tactctttgc
     5161 taagtctaag cagttgaagg cagttgggaa agcttccttt attccagtaa gtttcgctgt
     5221 caacgaacca ttgctatttg cggcacccat gattttgaac ccttattttt ttattccatt
     5281 tttgttaaca cctgttgtaa acgtttgcct gttcaagttt tttgtcgatg tattgggcat
     5341 gaacagcttt atgtacgttt tgccttgggc aactccagca ccgatcggtt tggttctagg
     5401 aacgggcatg ggtatcttag cttttgttct agcagcggtt ctaatcgtcg ttgacttcct
     5461 gatctatcta ccattttgca aagcatacga tgctgtctta gtagagaaag aagcacaaaa
     5521 agcagaggtg atcgcaaccg aggaaggtgc ttctgccgat gtacgtgagg aagtacctgt
     5581 ggcagcgagt caagttatca gcgatgcaaa taaagaaatc aatgtgttgg tactttgtgc
     5641 tggcgcaggg acaagtgcga tgttagctaa tgctttaaaa aaaggtgcat ctgaatcgaa
     5701 agcaccgatc agtgcaatgg cgggtgctta tggttctcat tacgaaatgc tgcaggattt
     5761 tgatatggtg gtcttagcac cgcaagtcaa ttcttactat gatgatatca aagaagacac
     5821 cgatcgctta ggaatcaagc tggttgcgac aaaaggagca gaatatatcg gattaacgag
     5881 agatcctgaa aaagctgttc agtttattct gaaccaattt caataaagga ggagccataa
     5941 tgaaaatggg aatttggttg cgtaaagctg ttttatttgc agctgtgtta ggggctttgg
     6001 gggctgtttc aaaaactgtt gaagcatctg ccagcgaatt tattcgcgga gcagatattt
     6061 cgatcctgtc agatatggaa aaatctggtg caaaatatta tgaagacgat atttcaaaag
     6121 atgccttaga gattttaaaa aataatggtg tcaactatgt tcgactgcgt ttgtggcagg
     6181 atccttataa tagtaatgga gaaagttatg gagcagggac aaatgatctg gaaacgacga
     6241 ttgaccttgc aaaacgagca aaaaatctgg gacttaaggt gctgctggat tttcactaca
     6301 gtgatttttg ggttgatcct gggaaacaga atcttcctaa agcatggcaa ggtctgacct
     6361 ttgaagaaat gaatacggca ttgtacgact atacaaagaa cgttctgtcg gaaatgaagc
     6421 agctggacgt ttatcctgat atggttcaga tcggcaatga actgaatagc ggaatgctct
     6481 ggccttatgg caagtcttgg ggagaaggcg ggggagaatt tgatcgattg gctgcttttt
     6541 tgaagtctgg tatccaagct gttcgagaca ctcaacctaa aacaactcca gtaatgctcc
     6601 acttagctga tggtggcgat acaggagcat ttacttggtg gtttgatgaa atcacgagtc
     6661 gaggtgtgtc atttgattta attggagtgt cgtattatcc gtattggcac ggttcaatga
     6721 atgaacttca aaccaatatg aatgcgatca gccaacggta tggtaaagaa gtgatcgtag
     6781 ttgaaacagc ttatgggcat acaacagcga atgccgatac tatgccaaat gcttttggtg
     6841 aagccgaagc agctgcgggg ggatatgaac caacaccgac aggacaagca gagtatttgc
     6901 tggatcttgc cgatcggatt caggcagtac cgaataatcg cggggcgggc ttcttctatt
     6961 gggaacctct ttggtacaac ggaaatgtga gttgggcaac acaagcaggt atgaattatc
     7021 tcagtgttca atccactatg ggaaacgaat gggacaacca agcgatgttt gattttgctg
     7081 gaaacgcctt gccaagcttg cgtgcgttta aacaagcagg ggcgcaaata aatctagtta
     7141 aaaatgcaag tttcgaaagc gatggaccaa cgaccagtcc ttccaattgg caaacctggt
     7201 ttcaacaagg aaccgctaag ggtacgatca aaacggaagc gggcactttt tttggggact
     7261 acaaactaac tttttggcag gatcaagcat acgaggcatc agcttatcaa aatgttagtg
     7321 gcataagcaa aggaacttac acattgagtg cctatgtaat gtctggggga gagtttgata
     7381 ctaatcagtt atatatcaaa aattatggtg gagcggaact aaatactgcg gttgttaaaa
     7441 caagtgaatg gacaaaaatt acaatcaaag acattccaat tacaacaggt gtttgtgaaa
     7501 ttggggttta tagcaaggct tcaggaaatg cttggacgaa cgttgatttt gtttcattgg
     7561 tgaaacaata gactgcggat cgtttcgatt caagatcgga ggttcccttc agccacatcg
     7621 ttagcgtaga aggcaggaat cgaacctgca cctcgccttt gccaaaaata accagaaacg
     7681 atttccagtc atttttacca aaacgagtaa agcaccagac ttcatctaca ctttgtatta
     7741 tgctaaagag agttcaacga tttcgtaaga agcaatgaaa aaattgcaaa aagttgtgca
     7801 gataacataa agtttgttta agaaagggta accttagaat tccgtcgtga atatgatttg
     7861 agcacttaga tggatagaaa aaagttctcg gaaaacagtt cgtttttcga gaacttcttt
     7921 ttatgataag aatgaaacaa attaacttat attatagtct cttttggtag ggaaccatct
     7981 tatttttaat gaaaatcaag cattttagct attgaagaat gttgatttat agggattccc
     8041 cctccatatt atgttggctc tttattccca ctccactgtt gctggtggtt tagaagtgat
     8101 gtcatacacg atacggttga cgtgggctac ttcgtttacg atacgtacag agattttttg
     8161 caagacttcc catgggatac gggcaaagtc agcggtcatt ccgtcgattg aagttacagc
     8221 acggataccg actgtgtagt cgtaagtacg tccgtcaccc ataacaccga cagagcggat
     8281 ccctggaaga acagtgaaat attgccagat atcacggtca agtccagcag ctgcgatttc
     8341 ttcacgcaag atagcatcag attcgcgtac gatttctagc ttgtcttcag tgatttcgcc
     8401 aagaacacga ataccaagac caggaccagg gaatggctga cgccaaacga ttgaatcagg
     8461 catgcctaac tgagtcccta attcacggac ttcgtctttg aataatgtgt ttaatggttc
     8521 gatcaattca aattgcatat cttctggcag tccacctaca ttatggtgag atttgatcgt
     8581 ctgtgctgtt tctgtaccag attcgataac gtcagtatac aacgttcctt gtgctaggaa
     8641 agagacgcct tcttcaccag caagttttgt tgcttcgtca tcaaaaacat atacgaattc
     8701 attcccaatg attttacgtt tttgttctgg gtcagataca cctttcaact tgtctaagaa
     8761 gcgatctctt gcattgactt tgatgatgtt caagccgaat tttccgccta gactttccat
     8821 tacttgttcc gcttcgcctt tgcgtagcag accgtggtca acaaagatag atgtcaattg
     8881 gtcaccgatc gctttttgta aaagaacccc aacgacacta gaatcaacgc cacctgaaag
     8941 accaagcaag acttttttgt caccaacttg ttcacggatt ttttctactt gcatatcgat
     9001 aaagttatcc attgtccagt taccttcaca gtgacaaaca tcaaaggcaa agtggcgcag
     9061 caattcgatt ccgtattcgg aatgacgtac ttctgcatgg aattggatac cataaaaatt
     9121 acgtgttgtg tcttggatag aagcaatcgg acaatctttg cttgttccga ctgtttcaaa
     9181 gccttcagga actttagtta ccaagtcacc atggctcatc catactgttt gttgttttgg
     9241 tgtttcttta aacaagacag cttgatcgct tgttacttct aacaaggctt tcccatattc
     9301 gcggttttta gcaggctcta cggaaccgcc aagtttgtac gtgatcaatt gcataccata
     9361 gcaaatccct aatacaggaa tacctaaatt gaagatctca ggatcgatcc caaatgcatt
     9421 ctcatcatat acactgtttg gtccgccaga aagaatgatt ccctttggat tcatcgcttt
     9481 tacttcttct gcagtaatgc ggtggctcaa taattcggaa aagactccaa gatcacgaat
     9541 acgtcttgtg atcaattggt tatactgact accgtagtct aatacgataa tcttttcaac
     9601 gttcgtcata tcggcaacgt ttgtcaaatt tattacccct atccctttaa attttatctc
     9661 aaaatagtca aactatttta aaatgctaat attttcgcat gaaaatcgaa tcgatgatat
     9721 gattttcagt tttatggaga attatatcgg ctcttcctcg tgtaggcaag atatattcct
     9781 gtaaattttt caaattcaca tttttccata catcttttgc cattttaaag gcttcttcac
     9841 gatctccgat agcaaaacga taataataat tctctggatt ttggaaagcc gtatccagta
     9901 acgcaccaaa acgatccaaa taccattttt cgatcagcac gggatcggca tcgacataga
     9961 cagaaaaatc aaaaaagtcg ctgacgtaga tttgctgatt cgcaggtaat tgaagcgtat
    10021 tgatcccctc aacgatcaag atatcaggct gctggatcaa ttcgtacttg tctggaatga
    10081 tatcatacac atcatgtgag taaacaggta cttttatctc ttcctgacca gattttacac
    10141 ggttcaagaa atcgatcagc atctccatat cataactttc aggaaatcct ttacggtcca
    10201 tgattccacg ctcttttaat atacgatttg gataaagaaa tccgtcagtc gtgatcagct
    10261 gaacgtttct tcgtttgaag gtacgagaca aaatcgtctg cagcagtctg gctgttgtac
    10321 tttttccaac ggctacactt ccggcgatcc cgataataaa aggcggtact tctacgtatt
    10381 tatgaagaaa taaaccttta ctcaagaaca aagattcaaa ttctttcata taaagataaa
    10441 tcagatgagc aagaggtaca tagatatctc gaacatcctg catggaaatg cggtcattca
    10501 aacttttgat attatctagt tcatcttgtg tcaaaggtgc ttgcccatta cgataaaatt
    10561 ctcgccattc ttctcttgcc acacggtagt agttcattgg atcattcata ttagcctcct
    10621 gcgttctccc atcaatggaa ctattttaac acaaaataaa cgcaaacccc accacttaag
    10681 agagattgcc gatctttttg tataatcttt ataatagaaa gaaacggagt cttattcttg
    10741 acttgcgcac ggatacataa aaggatagac tgttgtcatg aaagaaatga gaaggatgga
    10801 agacatgaca aatcattact attctgaacg cccagatacg gcgcacgatt ttgaacaatg
    10861 ggcttttgag ttaaaaggca gaacctttca atttgtaaca gattctggtg tattttcacg
    10921 tgaaacagtg gattatggtt cacgtgtcct gattgatgct ttcgattggg aagagttacc
    10981 tgatgaagga aagatcttag atgtgggatg cgggtatgga ccgattgggt tagctttggc
    11041 atttgcgaca cagcggtttg ttgaaatggt cgacattaat gaacgagcgg taagtctagc
    11101 gcaaggaaac gcaaagcgta accaaatcga acatgttgat atccatcaat cgaatatcta
    11161 tgaagcagtt catgaagaaa cctatgcagc tatcgtaagc aatccgccga tccgtgctgg
    11221 aaaaaaagtt gtacatgaaa ttttgacagg cgcatatcct cggttgaaga agggcggtac
    11281 actgacaatt gtgatccaga agaaacaagg agcgccgagt gcgcaaaaga aaatggaaga
    11341 aacatttggt aatgcagaaa tcgtcacaaa agataaagga tattatatca tcaaaagtgt
    11401 gaaagaataa ctagctgaaa aatagtggag cgacgaaaga gtggaactgg gcgtgcatgt
    11461 acgggtacgt atcagttctt tttttgttga gaagttttta aaaacaaaga actatttttt
    11521 cttcaaaaag aaatagtagg aacagagtcg ttgtttttta cgataaaata aataagatac
    11581 agaacttgta tagaaggctg ccaagtcggt agataaagga ggtttttatc aaaaaacata
    11641 ctttcctttt ttctctattc ttttcttggg ggttttggta cactgatagg gaatttagat
    11701 agaagggagc caataggatg aagctgacca tcagagatat cgcagaaatg gcaggcgtat
    11761 cagtaacaac tgtctcacag attttgaata acaaaggaag cagatttagt gataagacaa
    11821 gaaaaaaagt gttgtcgatc gtgaatgaac atcattataa gcccgattat ttcgcttcaa
    11881 atgtgatcaa tcgacattca aagaccatcg gaatgatcgt ccctgatgtc actgatttct
    11941 tcttctccaa actgatcgaa ggtgtagaga gctatttgaa tcctttgggc tatgtgatca
    12001 tgttatgcaa ttctcgtcat agccaagaaa atgagatcaa gtatttacaa gagctttccc
    12061 accgctcagt ggacggaatc ttgctggcca caccaaatat tttacctgag aaatatgcat
    12121 tagacagcgg tttttatcaa aaaatgccaa tcattttaat cgatcgcgga ttgaatcgtc
    12181 gggataacgg acggcttatc gtaaaggaat atgagggcgc ctatcaagct gtttcgctgt
    12241 ttattgaaaa tggccatacg aagatcggca tgctgaagga aaccactgga tactatcaat
    12301 tagaagaacg tttcaacgga tatcgtcatg cgctgaaaga tcatggcctg ccttttaatg
    12361 ggaaatttgt tgaacatgga gagttgactg tccaaggtgg atatactgca tcaaaaaaat
    12421 tactcaaaca taaagatatc acggctattt tttgcgggaa tgatgccatg gcgatcggat
    12481 gctatcaggc gatcgatgaa ttgggcaaaa aaatcccaga agatatctca gtcataggat
    12541 tcgatggatt aaagctgtca gaatacatga ttccgcaatt gacgacagtg cagcagccaa
    12601 gttttgatat tggattttat gctgcacgtt tcctgatcga tacgatcgaa tttcctcaaa
    12661 gaaaagttcc gaacaaagtg ttcgaaacaa aattaattat tcgtgagagc gtaaaagtat
    12721 tgacaaaggg ctaactcggc ggtatattgc ttatgtaagc atttacaaag gctagttagt
    12781 gcttttttta tttttagtaa accagtttac taaagtggtt tacctttcct aaaaataatg
    12841 tataataccg aatgaagaca aggaggatca ggcaatgcga atggtggatc tgattgaaaa
    12901 aaagagggat ggaaacgagc tgtcgaaaga ggaaatcgag tacatcgtca cgaactatac
    12961 gaatggaaag attccagatt atcaagtcag tgcattattg atggcaattt tttatcaaga
    13021 catgacaaat gaagaaatca cgaacttaac attggcgatt gctaattctg gtgatgtcat
    13081 cgatttgagt tctcttgaag gaataaaggt agataagcat tcgacaggtg gcgttggtga
    13141 cacgaccaca ttgattttag cccccctagt agctagtgtc ggagtgacag tggctaaaat
    13201 gtctggacgc ggtctaggtt acacaggtgg tacactagac aagctagaag caattcccgg
    13261 atttcagatc gagctttcgg atgaagaatt tgtacgcatt gttaatgaaa gtaaagtagc
    13321 ggtaattggt caatcaggaa acttggcacc ggcggacaaa aaattgtatg ccttgcgtga
    13381 cgtcacagcg accgttgatt ctcttccgct gattgccagt tcgattatga gtaaaaaaat
    13441 cgcggcagga gcagatgcaa tcgttttgga tgtgaccact ggagacggtg ctttcatgaa
    13501 gaacatcgaa gatgcaagac gtctggcaaa gaccatgacg agcattggga aactggctaa
    13561 ccgagaaaca gtggcggtta tctcagatat gtcagaacct cttggcgaag ctatcggaaa
    13621 tagtctagaa gttgtcgagg caatcgaaac attacaagga aatggtccag aagatctagt
    13681 agaaatgtgc tacgccttag gaagccaaat ggttgttctt gctggcaaag ctaagacaac
    13741 cgatgaagca agaacgttgc ttcaagaagc tctggaatca ggtaaagcgt tagcaaaatt
    13801 caaagaaatg atccaaaatc aagggggaga cccagcgatc gtggaacatc cagaacgaat
    13861 tttgactgca cgctacacaa tggagcttcc tgctaaacaa agcggagttg tgtcaaaaat
    13921 cgtggcaaat gaattaggaa ttgctgcaat gatgctaggt gctgggcgta aaaccaaaga
    13981 agaagatatt gatcacgctg ttggattgaa actgcataaa aaaattggtg acactgtgac
    14041 aaaaggtgag tcactattga cgatctatag taacgacgaa gaaatcactt ctgtcattga
    14101 attattatat aaaaatattg aaattggtga atcggcaatg aagccaacgt tgattcatga
    14161 catcatcaca gaataaagga aaaaagcgga ctttgccgtt ttaaagaaaa ggggaaaaga
    14221 aatggaacta aaccgaatga tcgaccatac tattttgaaa gcagacgcat ctaaagaagc
    14281 tgtgatgcaa attatcgaag aagcaaaaaa ataccatttt tattctgtct gcatcaatcc
    14341 agcatgggtt gcgctagcga aagaacaatt acaaggtgag ccagtagccg tatgtacagt
    14401 aatcgggttt cctctaggag caaacacacc cgagacaaaa gcatttgaaa caacagatgc
    14461 catcaataac ggtgcagatg aagtcgatat ggtcatcaat atcggtgctt tgaaatcaaa
    14521 ggatgatcaa caagtccaaa aagatatcga agcagttgtt gaagcagcaa aagatcgtgc
    14581 gcttgtcaaa gttattattg aaacatcgtt attagataga gaagaaatca tccgagcttg
    14641 cgaaatagca aaagctgcag gcgctgattt tgtcaaaaca tcaactggtt tctcaacagg
    14701 cggagcaaag gtagaggatg tccgtttgat gcgtgaaact gtaggaccag aaatgggtgt
    14761 caaagcttcc ggcggtatcc ataacgaaga agaagcaatg gcaatgatcg aagctggtgc
    14821 tacaagaatc ggaacaagcg caggagtagc aatcgtatct ggttcaactg gtgaaggcta
    14881 ttaatttttt caaaggagaa gctcatggca gaactcaaac aagaatggat caacacagct
    14941 attgaagcgt tagataaagc ctatgttcct tattcccatt ttccggtagg cgcttgtctc
    15001 gtaacggaat caggcaagat ttatcaaggc atcaacattg agaatgcatc atttggatta
    15061 acgaattgtg ccgagcgcac agcttttttc aaagcagtat ctgaaggaga aaaaacattt
    15121 acgcatctag tcgtagcggg gcacaccccc gatcctatct ctccttgtgg tgcatgtcga
    15181 caagtaatgg cagaattctg ttcccctgat atgccggtta cgctagtagg agacaatggt
    15241 gtgacgaagg caacgaccgt tcgtgcactg ctgccttatg catttactga aaaagatcta
    15301 taactcatgt tcattcagtc agctgactgt tgaaaaataa aacaattatt ttaggaggct
    15361 ttacagaaat gaaaaaagca aaattatttg gtttaggtgc tgtcgcacta gcagcagggt
    15421 tatttttagg tgcatgcggc aacaacggct ctactgattc atccggtggc aaaagctcat
    15481 cagacacaac aactgcagca ttgatcacag acacaggtgg tgtagacgac cgttcattca
    15541 accagtcagc ttgggaagga ctagaaaaat gggggaaaga tcaaggactt tcacgaggaa
    15601 atgatggctt ccaatatttc caatcttcaa atgaatcaga ttacatccca aatattgacc
    15661 aagcattgaa tgctggattc aaaacgattt ttggtattgg ttataaacta aaaccagcga
    15721 tccaagaaca agcaacaaac aatacaggta cgaactttgt tattatcgat gatgtcatcg
    15781 atggattaga taacgttgta tctgcaactt tcaaagacaa cgaagcttct tatcttgcag
    15841 gtgttgcagc agcttatacg actgaaacaa acgtagtcgg ctttatcggc ggtgtaaaag
    15901 gtgaagtaat cgatcgtttt gatgctggat tcaaagccgg tgttgatgca ggtgcgaaag
    15961 aattaggaaa agaaatcaaa gtattgaatc aatatgctgg tgacttctct gctccagata
    16021 aaggacgttc aattgctcaa ggtatgtatg cacaaaacgc agatatcatc ttccatgctt
    16081 caggcggtac aggtaacggt gtattccaag aagcaaaatc attgaacgaa tcaggcgaca
    16141 aaaaagtttg ggttatcggt gttgaccgcg accaatcaga cgaaggcgaa tacacattaa
    16201 acggcgaaaa gaaaaacttc acattgacat ctacactgaa agcagttgga acagtagtag
    16261 aagatttggc acaaaaatca gcggacggta aattccctgg cggcgaacac actgtttatg
    16321 gattgaaaga agacggtgtt ggtctaacag aaggacaact ttctgacgaa gcgaaaaaag
    16381 cagttgatga agcaaaagaa aaaatcattt caggtgacgt aaaagttcca gaaactcctg
    16441 aagaaaacta aaaccatttc tatagccttg ttagacacat gtttgacaag gctatggttt
    16501 gattagaaca agaattgttc atttatccaa gctgccttgt gacagtttgg ataagggaac
    16561 aactccctga agtaatgttt gtttcaaaag aagaacgctc actaagcgtg aatcttagtc
    16621 aatcgttgcc ctagatagaa atcgttagga tgtgaaaaaa aggtggttca agaaaatatt
    16681 gtcatagaaa tgcagaatat taccaaagaa tttggaacat tcaaggcaaa tgacaacatc
    16741 aatttacaag taaaggcagg agaaattcat gcgttgcttg gagaaaacgg tgctggcaaa
    16801 tctacattga tgaacgtgct ttccggatta ttagagccga catcagggaa aattttgatg
    16861 cgtgggaaag aagtaaagat cacaagtccg accaaagcca accaattagg gattgggatg
    16921 gtccatcagc actttatgct tgttgatgcc tttactgtaa cagaaaacat cgtgttggga
    16981 agcgagccta gtcgcgcagg gatgcttgac cataaaaaag cacgaaaaga gatccaaaaa
    17041 gtttctgaac agtatggatt gtcagtcaac ccagatgctt acgttcgtga tatttcagtt
    17101 ggaatggaac aacgggtaga aattttaaaa acactttacc gaggagcaga tgtactgatt
    17161 tttgatgagc cgacagctgt attgacccct caagaaattg atgaattaat cgtgatcatg
    17221 aaggaattag tcaaagaagg caagtcgatc attttgatta cgcataagtt agatgaaatc
    17281 aaagcagtag ctgaccgctg tacagttatc cgccgtggaa aaggaatcgg tacagtcaac
    17341 gttaaagacg taacctcaca acaattagcc gatatgatgg ttggaagaac ggtttcattc
    17401 aaaacgatga aaaaagaagc gaagcctcaa gaagtcgttt tgtctattga aaatctagtg
    17461 gtgaaagaaa atcgtggatt agaagccgtg aaaaacttga acttagaggt tcgtgctggc
    17521 gaagtacttg gtatcgctgg aatcgatgga aacgggcagt cggagttgat ccaagctttg
    17581 actggtttgc gaaaggcaga aagcggacat atcaagctaa aaggggaaga catcactaat
    17641 aaaaaacccc gaaaaatcac tgaacatggt gtaggacacg tgccagaaga ccgtcataaa
    17701 tacgggttag tcctagatat gacattatct gaaaatatcg cactacaaac ctatcatcaa
    17761 aaaccttaca gtaaaaacgg catgctgaat tattcagtaa tgaatgaaca tgccagagaa
    17821 ttgatcgaag aatatgatgt tcgaacaacg aatgaacttg ttcccgcaaa agctttatca
    17881 ggcggaaatc agcaaaaagc aattatcgct cgtgaagtcg accgagatcc tgatctgttg
    17941 attgttgcaa atccaactcg tggactggat gtaggtgcga tcgaatttat tcataaacgt
    18001 ctgatcgaac aaagggacaa atacaaagca gtgttactga tcagtttcga attagaagaa
    18061 attttaaatg tttcggatcg tattgctgtt atccatgaag gagaaattgt cggtatcgtt
    18121 gatccgaaag aaacatctga aaatgaatta ggtctattga tggcgggcta taccttagaa
    18181 gaagcacgaa aagagttaga aaagggggct ggcgcacatg aatgatcgca gcgaaaaatt
    18241 ccgtaattta ttagtgcctg tactttcagt tgttttagga ttgatcttag gcgcgatatt
    18301 gatggctgct tttggttttc agcccatcca aggttattct tctatgctag atgcagccct
    18361 tggttcgcaa agagggatag gtgaaacgtt aaggcaagca acgccgttga tttttacagc
    18421 attaggtttt tctgtagcta actcagctgg gtttttcaat attgggcttt ctggacaagc
    18481 actttgcggg tggatcgtca gtgtctgggt ggcacttgcg tttccagatc tgccaaaagt
    18541 tgttttgctt ccgttatgcg tgatcttagg tgccttagct ggtgcgatgg cagcggctgt
    18601 tccaggttta cttcgagcat tttttggaac aagtgaagtg atcgtaacaa tcatgatgaa
    18661 ctatatctta ttatacgtaa gcacatttgc tttacatgaa gtgatgcctg aaagctatcg
    18721 ctcaagtttg gactcttcca atatgatcag tcaaaatgcc tcactgcggt taccatggtt
    18781 aacacaagct tttggcggct ctcgcgtaaa ctcaggatta tttttagcaa tcatcgcgct
    18841 tattttcgtt tggctgctga tgaagaaaac gactttagga tttgaaatcc gttctgttgg
    18901 attgaatcct tttgcttctg agtatgcagg aatgagtagt aaacgtacga tcgttttgtc
    18961 gatggtcatt tctggcgctt tggcagggct tggcggtgtg gttgaaggat taggaacgta
    19021 tcaaaacttc tttgttcaaa cgacttcgct atctattgga tttgacggaa tggctgtttc
    19081 cttgcttggt tctggatcag caatcggtat tcttttatca gcgatcttgt tcagtatttt
    19141 gaagatcggt ggacttggca tgcagacagg agcaggtgtt ccgtttgaga tcgtcaatgt
    19201 atcgattgct ttgattatct tctttgtcgg gatcaacttc ctgattcgtt tcgtcatggc
    19261 taaagtcttc caagggaaaa aacaagaaga gatcgttgcg acaatagaat cgaaaccaac
    19321 gaatcaaaac catgaagggg gcgaactata atgtcgacta tcgaattaat tgccttgatc
    19381 cttacttcta cactggttta ttcaacaccg ctaattctaa cgtcactagg cggaactttt
    19441 tctgaacgaa gcgggatcgt aaacgtaggg ctagagggaa tcatggtcat gggtgctttt
    19501 agttccatcg ttttcaactt gacgatgagt ccatcacttg gttctatgac accatggatc
    19561 gctattttag tgggtggcgt cgtaggtatc ctattttctc tgattcacgc agtagcaaca
    19621 gtcagtttgc gtgctgacca tattatcagc gggacagtaa ttaatctgat ggcacctgct
    19681 ttaggtgttt tcttgatcaa agcatggtat ggtaaaggtc aaactgataa catcacgcag
    19741 aatctggggt atttttcttt cccaggatta gcagatattc cggtcattgg accaattttc
    19801 tttaaaaata cattcttacc tgcttattgt gctattttga ttgctgtcct agcttgggtg
    19861 atcatcttca agacgaaatt cggcttgcgc ctccgttcag ttggtgaaaa tcctcaagct
    19921 gccgacacat taggtatcaa tgtatatgct atgcgttatt caggcgtact gatttctggc
    19981 ttgttaggcg gtatgggcgg tgctgttttt gctcagtcga tttccggtaa cttttcagct
    20041 ggtacgatcg ttggacaagg gttcatctcc atggctgcta tgatctttgg taagtggaat
    20101 cctttagggg caatgggtgc ctcattgttc tttggttttg ctcaaagctt gagtatcatt
    20161 ggcgcacaat taccagttat ttcttcgatt ccgccagtat tgctgcaaat tgctccatat
    20221 ttgctgacaa tcattgtgct ggttgttttc ttagggaaag cttctggtcc aaaagcaaac
    20281 gggaagaact atatcaaatc aaaataatct tttagcgaat aaaaagggtg ggttacggga
    20341 agtgtttttc gtctcgccct ttttgtttta aaggcaataa aaataaaaag aaagaaggaa
    20401 taatcatgtt taaacgcgta catctgatcg tcatggattc agtaggaatt ggagaagcac
    20461 cagatgccga taagttcggt gatgttggtt ctgatacatt aggacacatt gcgaaagaag
    20521 caggattgaa cgttccacat ttagagcagc ttggtttagg aacgatccgt cctcttgaag
    20581 gtgtggaatc agtgaaagat catcaaggct atgcgacgaa attagaagaa gtttctgtag
    20641 ggaaagatac gatgacaggc cactgggaaa tcatgggatt gaatatcaaa actccatttc
    20701 gtgtgtttcc taatggattc cctgatgatc tgatcaaaca aatcgaagaa ttttctggcc
    20761 gtaaagtcgt atgtaacaaa ccttatagcg gaacagctgt tattgacgat tacggtgaac
    20821 accaaatgaa aacaggcgat ttaattgtat atacttctgc cgatcctgtc cttcagatcg
    20881 cggcacatga agaaatcatt cctttagaag aattatatcg gatttgccaa ttcgttcgtg
    20941 atataacaaa agatgatccg tatatgattg gccgaatcat tgcacgtccg tacgtgggtg
    21001 aaccaggaga cttcacacga acaagcaatc gccatgatta tgcgttgaat ccattcggac
    21061 acacagtcct tgattctttg aaagaagcag gaaaggacgt gattgctgtt ggaaagatca
    21121 acgatatttt caacggacaa ggaatcactg aatctgtccg cacgaaaagc aatatggatg
    21181 gcgttgacca gctgctgaaa gttatgaaaa acgatttttc aggaatcagc tttaccaatt
    21241 tagtagattt tgatgcatta tacggtcatc gtcgggatgt agtcggctat gcgcatgcga
    21301 ttgaagaatt tgacttacga attccagaat tgcttgaagc gatggaagaa gacgacttgc
    21361 taatgattac agccgatcac ggaaacgatc cgacgtttac tggtaccgat catacgcgag
    21421 aatatgtacc gttattggtt tacagtaaaa aaatgaaaga acaaggggca ttgcctcaag
    21481 gatattattc agatatttca gcaacgattg ctgagaactt tagtgtttct tcgactgaaa
    21541 atggcgaaag cttcttgaaa ttattaaaat aatcttttta aataggagaa agcaaatgac
    21601 caagttaagc gagatgttac aagaaacgac ccagtttatc cttgataaag gagtaaaaga
    21661 aattgatttt ggtttgattc taggttcagg attaggtgaa ttagcagatg aaatcgaaga
    21721 tgctgtaatg atttcttatg atgagatccc gttctttcct gtttcgacag tagttgggca
    21781 tgcaggaaag ctagtttatg ggtctctggc aggcaagaaa gtgttagcca tgcaagggag
    21841 attccactac tatgaaggtc attccatgca aacagtgacc tatccagtac gtgtgatggc
    21901 cggactaggg gcccattcag tcattgtgac taatgcatgt ggtggtgtca acgaatcatt
    21961 tgtaccagga gatttgatgc tgattaccga tcatatcaat tttactggtg aaaatccttt
    22021 gatcggtccg aatgaagatg caatgggccc tagatttcca gatatgagtc aagcttatac
    22081 gcctgactat caagcaacag caaaagcagc agcagaaaaa ctaggtttgg atcttaaaga
    22141 aggcgtatat atgggctatt ctggaccaac ttatgaaacg cctgcggaga tccgaatgtc
    22201 tcggacaata ggagccgatg cagtagggat gtcgactgtt ccagaagtca tcgtagcagc
    22261 tcacagcgga ttaaaagtgt taggaatctc ttgtattacc aacttagcag caggtatgca
    22321 agcgaatctg agccatgaag aagttgtaga aacaactcag agagtcaaac aatcttttaa
    22381 agcattggtt aaagaaacat tgacattatt gtaataggag gaacaataaa atgagtgtgc
    22441 atattgaagc gaaagaaggc gagatcgccg ataagatttt acttccggga gatcctttgc
    22501 gagctaaata tattgcggag acttttttgg aagatccagt ttgttacaat caggtacgcg
    22561 gtatgttagg ctttaccggc aaatataaag gagaacgtat ttctgttcaa ggaacaggaa
    22621 tgggaatgcc atcagccacc atctatgccc atgaattgat ccagtcgtat ggtgtaaaga
    22681 aattgattcg agtaggtaca tgcggtgcat tgtcaaaaga cgtccatgtt cgtgatttag
    22741 tactagcaca aggtgcagcg accagttctt cgatgatcga aaaaaatttc caagcctttc
    22801 attttcctcc aatcagtgat ttcaatctat tactgaaagc atatgagata gctaaagaaa
    22861 agggctatac ggttcatgta ggaaatgttc tttcggaaga ttctttctac aaagatgatt
    22921 tgacagagac gttccaatta gctgaacttg gcgttttagg agtggaaatg gaagcagcgg
    22981 ctctttatta cttaggcgcg aaataccatg tgcagacatt aagtttgatg accgttagcg
    23041 accatttgat caccggtgaa gaaacaacag ctgcagaacg tcaatctacg ttcaatgaaa
    23101 tgatcgaagt aggattggaa acagcgatag cagaataaga aggaaatgaa aaagctgccc
    23161 tgcattaagt aataagaaca tccaagcaaa aatagctcct catgtttttg acttggctgg
    23221 acttattatt gcaaatgtaa gcttttgaac accgtttacc aagaggttgt gaatctgccg
    23281 tttaactctg agtcacaagg aacaatttca aaaaacagct tttcatgttt gatgaatatt
    23341 gtgacttgtg accgagaagt tggcaggaga aaaccgttta tacgaaaaaa gggactgtga
    23401 caaatcgtgt cacagtccct tttttatagt gttttttata ggtagtattt tttagcaact
    23461 gttaagtcat catttaattc ataaacaagt ggttgacctg ttggaatttc aagatccatg
    23521 atgtcttcgt cagagatacc ttcgatatgt ttagctaggg cgcgtaatga gttaccatgt
    23581 gcagctacta aaacagtttt gttgtctagt aatgctggag caatttcatc ttgccagaat
    23641 ggcaaagcgc gttctaatgt cactttcaag ttttctccac caggaatatc gcgtttgtct
    23701 aacattgcgt aacgacggtc atttgctgca gaaccttcgt cagtttcttc cattaatgga
    23761 ggaagagtat cgtaagaacg acgccagatg tgcacttgtt cgtcgccgta tttttcagct
    23821 gtttcttttt tgttcaaacc ttgtaattta ccatagtgac gttcgtttaa gcgccatgat
    23881 ttgatttgtg gtacccaaag ttgatctgaa tattctaaga tcaagttaca tgttttgatt
    23941 gcacgagtta aaacagaagt gtaagcaaca tcaaattcga ttccagcttc tttgattttg
    24001 cgtccgcctt cttttgcttc ttcgattcct tctggtgcta gatttacatc agcccaacca
    24061 gtaaattggt tcaatgcatt ccattcacta agaccgtgac gagaaaaaac taattttggc
    24121 atgtttagct ctccttttaa gataggttta tatttctgaa attttcagtt ctgttttatt
    24181 gtacaatttt ttggaagaga tttccatcga aaaattagat aagattctat gagaaaacaa
    24241 gtagagaata taaaaaaacg aacagtgccc acgcaactgt tcgtttttag gtattagatg
    24301 cataattgct cgatttcttt ttcaggtaaa ttgatatatt tataacatgt tccaactgaa
    24361 acaccgcatt ttgaagaaat aaattggatc gtttcttttt tttcataata gagacgtcgg
    24421 atttttttga tcgtttttgc atcgattttt ggtcttccgc cgatttttcc ttttttacga
    24481 gcttcatcta atccaactaa tgtacgttct ttgatcaatg cgcactccat gtttgctagg
    24541 ttttccatta attggaaata gatttttccc atttcgcgcg tatcgattcc ttcatcaaga
    24601 ctaatcaagt gcagcccgcg atctttgaat aattgcgtga gttctgttaa ttgccgcgta
    24661 gatttgccta atcgatgaag ttggcaaatg actagtgcat cgccttgttg catgtcagcg
    24721 atcactgact ccaaatgaac agcagtagaa tcattttgtt ccacttcata tgtctcatgg
    24781 aaaatttctt gacaaccaaa gtcagataat tgatcaacct gagttgttaa atcaacgtca
    24841 tttatggttg tgcgtgcata accaataatt ttcatggtga ttcctttcta gcagtgattt
    24901 tctaactgga tataacagta aaaatgtagc ataaagtacg tcaaaaagat agttttttcg
    24961 caaattttcc gctgtctttt tttattggat gatcaagcag tttttcttta tttacgacgg
    25021 attgcgtcaa gaatctcaac gcctttttct gagccgacaa ctactttaga aaccatgttt
    25081 aggaaaagcc catgttctac taccccaaca agttcatcta aataggtagc taagctttct
    25141 ggctgttcga tgacttctag atgcagatca atgatgtaat gaccaccatc tgtggttaag
    25201 acttctccgt tttcgtctgt acgcaagacg ggttggaatc ttttttcttc aaaaatatga
    25261 agtagctgtt gggagccata agggatcact tcaactggga gtgggaactt cccaagttta
    25321 tcgactaatt tagaagaatc aacgatccaa atcgtttctt ttgagtacgt agcgactatt
    25381 ttctcgaaaa gcaaagcagc gccgccgcct ttgatccctt gaaaatcagc actgatttca
    25441 tcagcaccgt ctattgtcaa atcgactacg gggacttcat caatactctt taacggtatt
    25501 cctaattttt ccgcttgttc ttttgtcgca tttgaagtag taactcctgt gatacgtaaa
    25561 ccttcttcac gcatacggcg accaatctct tctaccatat aataggcggt tgaaccggtc
    25621 ccgagaccta cgatcatccc gtcctttacg aatttcgcgg cttcaatgcc agctaattgt
    25681 tttaaattca tctttgcgtc cacctttttt tagtttgagc atcgtgcttt cctcactatt
    25741 ttagcgtgat ttacggtaaa aagataccat tcttttttat tccaataaag ctttttcaaa
    25801 aaaaattgca tctcttgttg acatgttttt tcattcgtga tattctaaca aaggtgcttt
    25861 atctacaggt ttctgtgaag aaacgtgctg actgtttatc aaagcatatt tgataaggat
    25921 gcagacattg catttatttt ttatcacaaa aaaagaacca cctggatgtg tggaactaga
    25981 cgcaaatcgt gggaaggagg aaacaaggaa tgcctacaat taaccaatta gtacgtaaac
    26041 ctcgtaaatc aaaggtggaa aaatctaatt cacctgcttt gaacaaagga tataacagct
    26101 ttaagaaaac tcaaactaac gtaaactcac ctcaaaaacg tggggtagca actcgtgtgg
    26161 gtacaatgac acctaaaaaa ccgaactcag ctttacgtaa atatgcacgt gttcgtttgt
    26221 caaacttaat tgaagttact gcttatatcc cagggattgg tcataactta caagaacaca
    26281 gtgttgtatt attacgcggt ggtcgtgtaa aagacttacc aggggtacgt taccatatcg
    26341 ttcgcggtgc tttagatact gctggtgtta acgaccgtaa acaaagccgt tctaaatacg
    26401 gaactaaaaa acctaaagct taattttaat tattaaacaa atgaaacttg cttctactat
    26461 atagaagtaa ttactatgaa caatatttcg gaaggaggag ttacggatgc cacgtaaagg
    26521 tcctgttgca aaacgtgatg ttttaccaga tcctatttat aactcaaaat tagtaactcg
    26581 cttgatcaac cgtgtaatgg ttgacggaaa acgcgggatt gcagctaaca ttatctataa
    26641 tgcctttgac ttgatcaaag aatctacagg taacgatcca ttagaagtgt ttgaacaagc
    26701 aatgaaaaac gttcaacctg ttcttgaagt taaagcacgt cgtgttgggg gttcaaacta
    26761 ccaagtacca gttgaagttc gccctgaacg ccgcgttact ctaggtcttc gttgggtagt
    26821 aaactacgct cgcttgcgcg gtgaacacac aatggaagaa cgcttagcaa aagaaatcat
    26881 ggatgctgca aacaacacag gtgcttctgt taaaaaacgt gaagacactc ataaaatggc
    26941 tgatgccaac cgtgcgtttg ctcactatcg ctggtaagat tctctctgtt tggtcggctt
    27001 ttataagtcg acctcagata gtgtccttat ttttagcaac tagatagtaa agagaggaga
    27061 aacaaggaga atgaccagag aattttcttt acaaaacact cgtaatatcg gtatcatggc
    27121 ccacatcgat gctggtaaaa cgactgctac tgaacgtatc ttgtactata ctggtaaaat
    27181 ccataaaatt ggtgaaactc atgaaggagc ttcacaaatg gactggatgg aacaagaaca
    27241 agaacgtggt attacgatca cttctgctgc tactacagca caatggaagg gtcaccgaat
    27301 caacatcatc gacacaccag gacacgtaga cttcacagtt gaagttgaac gttcattgcg
    27361 tgtacttgat ggtgctgtaa ctgttcttga tgcacaatca ggtgttgaac ctcaaactga
    27421 aacagtttgg cgtcaagcaa caacttatgg tgtacctcgt atcgtatttg caaacaaaat
    27481 ggacaagatc ggtgctgact tcttatattc agtatcaact ttacatgacc gtttgcaagc
    27541 aaacgctcac ccaatccaat taccaattgg tgcggaagac gacttcacag gaattatcga
    27601 ccttgtaaca atgaaggctg aaatgtatac aaacgatctt ggaacagaga tcgaagaaac
    27661 agaaattcct gaagaatacc gcgaattagc tgaagaatgg cgcgaaaaat tggttgaagc
    27721 tgtagctgaa actgatgaag aacttacttt gaaatactta gaaggtgaag aaatcactga
    27781 agctgagtta aaagaaggta tccgccgcgc aacagttaac gtagaattct acccagtact
    27841 atgtggttca gcatttaaga acaaaggggt tcaattacta ttggacgctg ttcttgatta
    27901 cttgccatca ccgttagata tcccagctat caaagggatt gaccctaaaa ctgatgaaga
    27961 agtagaacgt cctgcagacg acagcgctcc tttctcagct ttagcattta aagttatgac
    28021 tgacccattc gtaggtcgtt taactttctt ccgtgtgtac tctggtgttt tacaatcagg
    28081 ttcatacgta caaaacgcaa caaaaggcaa acgtgaacgt gttggtcgta tcctacagat
    28141 gcatgccaac tctcgttcag aaatctctga agtttacgct ggagatatcg ctgctgctgt
    28201 tggtttgaaa gacacaacta ctggggacac actatgtgat gaaaagagtc tagttattct
    28261 tgaatccatg gaattcccag aaccagttat ccaagtcgct attgaaccta aatcaaaagc
    28321 tgaccaagat aaaatgggtg tggctttaca aaaactatct gaagaagatc ctactttccg
    28381 tgctgaaaca aacgtggaaa caggcgaaac aatcatcgct ggtatgggtg aacttcactt
    28441 agatatcatc gttgaccgta tgcgtcgtga attcaaagtt gaagctaacg taggtgcgcc
    28501 tcaagtatct taccgtgaaa ctttccgtgc tggcactcaa gccgaaggta agtttgtacg
    28561 tcagtctggt ggtaaaggtc aatacggtca cgtatggatt gaatttacac caaatgaaga
    28621 aggtgctggt ttcgaatttg aaaatgcgat cgtcggtggg gttgttcctc gtgaatacat
    28681 tccagctgtt gaaactggtt tgaaagatgc tatggaaaat ggtgtcttag ctggctatcc
    28741 attagtagat atcaaagcta aactttacga tggttcatac catgatgtcg attcaaatga
    28801 aactgccttc cgtgttgctg cttctatggc attacgtgca gcagctaaga aagctaatcc
    28861 agtaatcctt gaaccaatca tggctgtaga agttgttatc cctgaagatt atttaggtga
    28921 tgttatgggt cacgtaactg ctcgtcgtgg acgtgttgaa ggtatggaag ctcgcgctgg
    28981 cggccaacaa gttgttcgtg cgatggttcc acttgctgaa atgtttggtt atgctacaac
    29041 tcttcgttct gctactcaag gtcgtggtac atttacaatg acatttgacc actacgaaga
    29101 tgtaccaaaa tcagtacaag aagaaatcat taagaaaaac ggcggaaaag ctgaataatt
    29161 tcttaaaacc ggatagtcct gcaaagagat tatccaaaga gactatattt ttaatatagt
    29221 ttcgtgtaaa ataattatcg atgatatggg catgtggtgt accttgtgta catcatatac
    29281 ctatcagaca aataaaaaat atattgtatt tttaggagga cattttaaaa tggcaaaaga
    29341 aaaatttgac cgttctaaac cacacgttaa cattggtact atcggacacg ttgaccatgg
    29401 taaaactaca ttaacagctg caatcacaac tgtactatct aagaaaaacg gcggccaagc
    29461 tatggcttac gatcaaatcg atggtgctcc agaagaacgc gaacgcggaa tcacaatctc
    29521 aacagctcac gttgagtatg aaacagatac acgccactac gctcacgttg actgcccagg
    29581 acacgcggat tacgttaaaa acatgatcac tggtgctgct caaatggacg gagctatctt
    29641 ggtagtttct gctgctgacg gcccaatgcc tcaaactcgt gaacacatcc tattgtctcg
    29701 tcaagttggt gttccttaca tcgttgtatt cttgaacaaa gtagacatgg ttgatgacga
    29761 agaattacta gaattagttg aaatggaagt tcgtgaccta ttaacagaat acgaattccc
    29821 tggtgacgat gttcctgtag ttgctggatc agctttgaaa gctctagaag gcgacgcttc
    29881 atacgaagaa aaaattcttg aattaatggc tgcagttgac gaatacatcc caactccaga
    29941 acgtgacaac gacaaaccat tcatgatgcc agttgaagac gtgttctcaa ttactggacg
    30001 tggtactgtt gctacaggtc gtgttgaacg tggacaagtt cgcgttggtg acgaagttga
    30061 agttgttggt attgctgaag aaacttcaaa aacaacagtt actggtgttg aaatgttccg
    30121 taaattgtta gactacgctg aagctggaga caacattggt gctttactac gtggtgttgc
    30181 acgtgaagac atccaacgtg gacaagtttt agctaaacca ggtacaatca cacctcatac
    30241 aaaattctct gcagaagtat acgtgttgac aaaagaagaa ggtggacgtc atactccatt
    30301 cttcactaac taccgtccac aattctactt ccgtacaact gacgtaacag gtgttgttga
    30361 attaccagaa ggaactgaaa tggttatgcc tggcgacaac gtaacaatgg aagttgaatt
    30421 aatccaccca atcgctatcg aaaacggtac taaattctca atccgtgaag gcggacgtac
    30481 agttggtgcc ggtgtcgtta cagaaatcaa agcttaattc acttaagttt taaagtaaag
    30541 caacatttat tcggacgtaa tcgctcggaa cgaaggtctt aggatctttg ttccgagctt
    30601 ttttatttat tgcaggatca ggaaaaaaga caggagtatt ataaatagaa gaaacacgaa
    30661 ataaaagagt cagtattttc taaatgaaac tttatgaaaa caaaatgttt tttacaagaa
    30721 aaagccttgc tttgtgagtt tatatatatt ataatgacaa aggtgcgttt actttagtga
    30781 attgcacaga cgtaatcaga atgatttcgt cggctaagaa acgagaggtt gcgacacgcc
    30841 cggacgcttt gccacgaacg agcgtgacgg aaattttcgt ggagctatgt ctacttttca
    30901 aaataggcga aggagggaac aagatggcaa aacaaaaaat tcgtatccgt ttaaaagcgt
    30961 atgaacaccg tattttagat caatcagcgg ataaaatcgt ggaaactgca aaaagaactg
    31021 gagctagcgt gtctggtcca attccattac caactgaacg cagtctttac acaatcatcc
    31081 gtgcgactca taaatacaaa gattcacgcg aacaattcga aatgcgtaca cacaaacgtc
    31141 ttatcgacat tgtgaaccca acaccaaaaa ctgttgatgc tttgatgaag ctagacttac
    31201 catctggtgt aaacatcgaa atcaaattat aaaactaaaa caaaaataat ggaggtgtac
    31261 tcatgaccaa aggaatctta gggaaaaaag tgggaatgac acaaatcttc actgaatctg
    31321 gtgaattaat tccagtaact gttgtagaag ctactccaaa cgtagtacta caagtgaaaa
    31381 caatggaaac ggatggctac gaagctattc aagttggtta ccaagatatg cgtgaagttt
    31441 tatcaaacaa acctgcgaaa ggtcatgttg caaaagcaaa cacggctcct aagcgcttca
    31501 ttcgtgaatt caaaaatgtt gagctaggag aatatgaagt aggaaaagaa attacagtag
    31561 atgttttcca agcaggagac attattgatg taacaggtac tacgaaaggt aaaggattcc
    31621 aaggggttat caaacgccac ggacaaagcc gcggaccaat ggctcacggt tctcgttacc
    31681 atcgtcgtcc tgggtcaatg ggtccagttg cacctaaccg tgtatttaaa aacaaacgtc
    31741 ttgctggccg tatgggtggc aaccgcgtaa ctatccaaaa cttggaagtt gtacgtgtcg
    31801 atgcagaaag aaatgttatc ttgatcaaag gtaatgttcc tggagctaaa aaatctttaa
    31861 ttactatcaa atcagctgtg aaagctaaat aattgtagga agaaaggagg aactaaggaa
    31921 tgccgaatgt agcattattc aaacaagatg gaagccaaaa cggtgaaatc acacttaacg
    31981 aagaaatctt cggaattgaa cctaatgaaa gtgttgttta cgatgcaatc gtaatgcaac
    32041 gtgcgtcatt aagacaagga acacacgcgg ttaaaaaccg tagcgctgtt cgcggcggcg
    32101 gacgtaaacc atggcgtcaa aaaggaacag gtcgtgcacg tcaaggatct atccgctcac
    32161 cacaatggcg tggaggtggc gtagtctttg gaccaacacc acgttcttac agctacaaac
    32221 ttcctaaaaa agttcgtcgt ttagcaatga aatctgtatt gtcagaaaaa gttgctgaaa
    32281 acaacttggt agcaatcgaa ggcttgaact tcgacgcacc aaaaacaaaa gaattcaaac
    32341 aagttcttgc taacttatct atcgactcta aagtattggt tgttctagaa aaaggtaacg
    32401 actttgctgc tttatcagca cgtaacttac caaacgtttc tgtagtaact tctgataatg
    32461 tgagtgtgct agacgttgta tcaaatacaa aagtgttagc aacacaaact gctcttactc
    32521 aaattgagga ggtgcttgca taatgaactt actagacgta atcaaacgcc cagtgatcac
    32581 tgaaaaatca atgcttgcca tggatgacaa gaaatacact tttgaagtgg acactcgcgc
    32641 aaacaaaact ttagtaaaac aagcggtaga agctgctttt gacgttaaag tcaaaaacgt
    32701 gaacatcgtg aacgtacgtg cgaaattcaa acgtatgggc aaatatgcag gatatacaaa
    32761 aaaacgtcgc aaagcgatcg ttacattgac tgaagattca aaagaaatcc aattattcga
    32821 agctgctgaa taagcactaa tagataactc attaaattag gagggaaaaa cgtggcgatt
    32881 aaaaagtaca aacctacctc aaatggtcgt cgtaacatga caggttctga ttttgctgaa
    32941 atcacaacat caacacctga aaaatcattg ttacaaccat tgaaaaacca tgctggacgt
    33001 aacaacaacg gccgcatcac tgtacgtcat caaggtggcg gacacaaacg ccaatatcgc
    33061 gtgatcgact tcaaacgtaa caaagataac gttgttgcaa ctgttcaaac gatcgaatat
    33121 gatccaaacc gttcagctaa catcgcgtta gtacactatg aagatggagt gaaagcttac
    33181 atcttagcac ctaaaggact agaagtgggt atgaaacttg tttcaggtcc agaagcagat
    33241 atcaaagtcg gaaatgctct tccattagaa aacattccag ttggtactgt tatccacaac
    33301 attgaaatga aacctggtaa aggtggacaa ttgatccgtt ctgctggtac aagtgctcaa
    33361 gtacttggta aagaaggcaa atacgtattg atccgcttga actctggtga agttcgtatg
    33421 atcttagctg catgccgtgc tacaatcggt tctgtaggta acgaacaaca cgaattaatc
    33481 aacatcggta aagctggacg ctctcgttgg atgcgtaaac gcccaactgt acgtggtagc
    33541 gtaatgaacc ctaatgatca cccacacggt ggtggtgaag gtaaagctcc tatcggacgt
    33601 aaagcaccag tttctccttg gggtcaacca gctcttggtt acaaaactcg taacaaaaaa
    33661 gctaaatcag acaaacttat tgttcgtcgt cgcaaaaata aataattact ttgaccatgt
    33721 gtcgcacatt ttgagaggag gttcaccatg ggtcgtagtt taaagaaagg accttttgtc
    33781 gatgagcatt taatgaaaaa agttgaagct caagcagggg ttgaaaagaa aaaagttatc
    33841 aaaacttggt cacgccgttc aacagttttc ccaagttttg ttggatatac catcgcagtt
    33901 tatgatggac gtaaacatgt accagtttac atccaagaag acatggtagg acacaaatta
    33961 ggtgaatttg cacctacaag aacttatcgt ggacatgctg ctgacgataa gaaaacgaaa
    34021 cgctaatttg aggggaggat aaatccaaat ggcagaacaa atcacatcag ctaaagcaac
    34081 tgcaaaaaca gttcgcactt cacctcgaaa agcccgttta gtaattgacc ttatcagagg
    34141 taaaagtgtc gcagatgcaa tttcaatctt gaaattcaca ccaaacaaat ctgctggaat
    34201 cattgaaaaa gtattgatgt cagcagttgc gaatgcagaa aataactttg acttagatgt
    34261 tgaaaacttg gtagtaagcg aagcttttgt taacgaagga cctacaatga aacgtttccg
    34321 tcctcgtgca aaaggatcag cttctccaat caacaaacgt acaagtcata tcacggtagt
    34381 cgtatcagaa aaataaggag ggataatcag tgggtcaaaa agtacatcca attggaatgc
    34441 gtgtaggcat catccgcgac tgggatgcta aatggtatgc tgaaaaagag tatgctgagt
    34501 tcttacacga agatttaaga atccgtaaat ttatttcaac taaacttgct gatgctgctg
    34561 tatcaacagt tgaaatcgaa cgcgcaacag gtcgcgtaaa catttcaatt cacacagcta
    34621 aaccaggtat ggttattggt aaaggcggtt ctgaagtcga aagcctaaga aaagaattaa
    34681 acaaattaac tggtaaaaga atccatatca atatcgtgga aatcaaaaaa ccagatctag
    34741 atgcaaaatt agtaggtgaa ggaatcgcac gtcaattaga aaaccgtgtt gctttccgtc
    34801 gcgctcaaaa acaagccatc caacgctcaa tgcgtgctgg cgctcaaggg atcaaaactc
    34861 aagtatctgg tcgtctaaac ggagcagata tcgctcgttc agaaggatac tcagaaggaa
    34921 ctgttcctct tcatacttta cgtgcggaca ttgattacgc atgggaagaa gcagatacaa
    34981 catacggaaa actaggagtt aaagtgtgga tctatcgtgg agaaattctt ccaactaaaa
    35041 aaaacactga gaaaggaggg aaataatcat gttagtacct aaacgtgtaa aacaccgtcg
    35101 tgaattccgc ggaaaaatgc gcggggaagc taaaggcgga aaagaagtag cattcggtga
    35161 atacggtttg caagctgttg attcacattg gatcacaaac cgccaaatcg aagctgctcg
    35221 tatcgcaatg actcgttaca tgaaacgtgg tgggaaagta tggattaaaa ttttccctca
    35281 caaatcttat actgccaaag caattggggt acgtatgggt tctggtaaag gggcacctga
    35341 aggatgggtt gcaccagtaa aacgtggtaa aatcatgttt gaaatcgcag gcgttcctga
    35401 agaagtagct cgtgaagcgt tacgtctagc ttctcacaaa ttaccaatga aaactaagat
    35461 cgtaaaacgt gaggaaatgg gtggtgaatc gaatgaaggt taaagaaatc agagaattaa
    35521 ccactgccga aatgcttgat caagaaaaac aattaaaaga agaattgttt aatcttagat
    35581 tccaattagc aacaggtcaa ttagaaaaca ctgcacgtat taaagaagta cgtaaatcga
    35641 ttgcacgcat caaaactgtg ttgcgtgaac aagctaagta attatggaag gaggccatta
    35701 agcgtatgac tgaaggaaga aatcaacgca aagtttatca aggtcgcgtg gtttcagaca
    35761 aaatggataa aacaattacc gttgctgtcg aaacaaagaa aaaccaccct atctatggca
    35821 aacgcatgaa ctattctaag aaatacaaag tgcatgatga aaacaacaca gcaaaagttg
    35881 gcgacatcgt gagaattatg gaaactcgtc cattatcagc tacaaaacgt ttccgtttgt
    35941 tggaagtagt cgaagaagca gttattatct aataacacga gattttccta tatagattga
    36001 atgcaaaatc tgaaaggagg atacacaacg tgatccaagc agaaagtcgt ttaaaagtcg
    36061 ctgataactc aggcgcacgt gaaattttga cgatcaaagt ccttggtgga tctggtcgta
    36121 aaactgctaa catcggtgat gtgatcgttg ctacggttaa acaagcaacg ccaggtgggg
    36181 ttgtcaaaaa aggtgacgtc gttaaagccg ttatcgttcg aacaaaatca ggagctcgtc
    36241 gtacagacgg ttcatatatt aaatttgatg aaaatgctgc agtgattatt cgtgatgata
    36301 aaagccctcg tggaacacgt atctttgggc cagttgcacg tgaactacgt gaaaacaact
    36361 tcatgaagat cgtttcacta gcaccagaag ttttataatc ttgagacact tatcaaagga
    36421 ggtgcgaaac agttatgttt gttaaaaaag gcgataaagt taaagttatc actggaaaag
    36481 acaaaaacaa agaaggcgtt gtactagcag cgttcccgaa aaaagataaa gttattgtcg
    36541 aaggtgtaaa tgtcgttaaa aaacaccaaa aaccttctca agctgctcct caaggtggaa
    36601 ttgttgagat ggaagcgcca attcatgttt ctaacgtgat ggtggttgac tctactggtg
    36661 tagctggccg agttggctac aaagaagtag acggcaaaaa agtccgtgtt tctaaaaaaa
    36721 ccggtgaagt tttagataaa taaaagttta aggaaggagg gtcttactga atgaaccgcc
    36781 taaaagaaaa atatcttaaa gaagtaactc catcattgat ggaaaaattt aactatagct
    36841 ctgttatgca aacacctaaa gttgaaaaaa tcgtcatcaa catgggtgtt ggtgatgcgg
    36901 tatcaaacgc taaaaactta gataaagcag ttgaagaatt aactttgatc actggtcaaa
    36961 aaccaatgat tacaaaagct aaaaaatcaa tcgctggatt ccgtttacgt gaaggaatgc
    37021 caattggtgc aaaagttaca ttgcgcggag aaagaatgta cgaattctta gacaaattgg
    37081 tttctgtttc actaccacgt gtacgtgact tccatggtgt aagtaaaaaa gcctttgacg
    37141 gtcgtggaaa ctatacttta ggtattaaag aacaattgat cttcccagaa gtagattatg
    37201 atttagtaga taaagtacgt ggtatggaca tcgttattgt aacaacagca aactctgatg
    37261 aagaatctcg tgagttgttg acacaattag gcatgccatt ccaaaaataa ttaaaggagg
    37321 cgaactacgt ggctaaaaaa tcaatgattg ctaaaaacaa acgtcctgca aaattttcaa
    37381 cacaagctta tactcgttgc gaacgttgcg gacgtccaca ttcagtttat cgtaagttcc
    37441 atctttgccg tatttgcttc cgcgaacttg cctataaagg tcaaattccc ggcgtgaaga
    37501 aagctagctg gtaaaaagta aattcgcata agaaggaggt aaaagatcaa tggtcatgac
    37561 agatccaatt gcagattttc taactcgtat ccgtaacgca aacatggtta aacatgagtc
    37621 tttagaagta cctgcatcaa aaattaaacg tgacatcgct gaaatcttga aacgtgaagg
    37681 tttcgttcgt gatgttgaat acatcgaaga tgacaaacaa ggcgtgatcc gtgttttcct
    37741 aaaatttggt aaaaacggcg aacgtgttat cacaaactta aaacgtatct ctaaaccagg
    37801 tttgcgtgct tatgtcaaag ctgacgaagt accaaaagta ttgaacggtt taggtatcgc
    37861 aatcatttcg acttctgaag gtgttgtcac tgataaagaa gctcgtgaaa aaaatatcgg
    37921 cggcgaagtt atcgcttacg tatggtaatc aagtagaatc tacaaggagg tgtctttaag
    37981 tgagccgtat cggaaataaa gtcgttgtta tccctgaagg agtaacagtt actcaagacg
    38041 gaaacaacgt tacagttaaa ggaccaaaag gtgaattgac tcgtactttt tcagcagata
    38101 tcaaaatgaa tatcgaagga aacgaagtaa ctttcactcg tccaaacgac agtaaagaaa
    38161 tgaaaacaat tcatggaaca actcgtgcga acttcaacaa catggttgta ggtgtcagcg
    38221 aaggtttcca aaaagcgtta gaactaatcg gggttgggta ccgtgcacaa ttacaaggat
    38281 caaaacttgt attgaacgtt ggttactcac atccagtaga aatcacacca ccagctggag
    38341 taactgttga agtaccagct aacacacaag taattgttaa aggtgcgaac aaagaagaag
    38401 ttggcgaatt agctgcaaac attcgtggaa ctcgtcctcc agaaccttat aaaggtaaag
    38461 gtattcgtta tgttggcgaa tatgtacgcc gtaaagaagg taaaactggt aaataatcgt
    38521 ttagaaaaat tgcatagaag ccagcagcag gaaaaaggca tttggctgaa gtttaccttc
    38581 aaaaaaatac atttttactt gtgccgcagc ttctattttt cgaaacgtat ttactgcttg
    38641 aaatagtagt acaataaaaa taaagaggtg acaattgtga ttacaaaacc agataagaac
    38701 aaaacacgtc aaaagagaca tcgtcgtgta cgtaacaata tctctggtac tgctgagcgc
    38761 ccacgcttga acgtttttcg ttctaacaaa aacatctacg ctcaaattat tgatgacgta
    38821 gcgggtgtaa cgctagcaag tgcctctacc ttggataaag aaatttcagg tggaacaaaa
    38881 acagaagcag cacaagctgt cggtaaacta gttgctgaac gtgcagctga aaaaggcatt
    38941 aaagtagtag tctttgaccg tggtggatac ctttaccatg gccgtgtgca agctttagct
    39001 gaagctgctc gcgaaaatgg actagaattt tagaagaagg aggaatacca ttcatggttt
    39061 atatcgatcc aaaacatttg gaattagaag accgcgttgt tgcaattaac cgtgtaacaa
    39121 aagttgttaa aggtggacgt cgtctacgtt ttgctgcttt agttgttgtc ggtgacaaaa
    39181 acggacacgt agggtttggt acaggtaaag ctcaagaagt tcctgaagca atccgtaaag
    39241 caattgaaga tgcgaagaaa aacttagttg aagtacctat ggttggttca acaatcccac
    39301 acgaagtaat cggtgctttt agtggtggta gaatcctaat gaaacctgct gtagaaggtt
    39361 ctggggttgc tgctggtgga ccagttcgtg ccgtattaga attagctggg gtagcagata
    39421 tcacttctaa atctttaggc tcaaatacac caatcaacgt tgttcgcgca acagttgaag
    39481 gtttgaaaca attgaaacgt gcagaagaag ttgcagaact tcgcggcaaa tcagttgaag
    39541 aattaatcgg ataaggagga caacaataat ggctgaatta aaagttactt taaaacgcag
    39601 tattatcgga cgtcctcaaa accaacgcga tacagttaaa gcgttaggtc ttacaaagat
    39661 taacagttca gtggttaagc cttctaatga agcaattaaa ggcatgatca atactgtttc
    39721 tcacctagtg gacgttgaag aaatttaatt tagaaaaatc ataactagaa cattaaggag
    39781 gtgccaaacc tatgaaactt catgaattaa aacctgctga aggttcacgc caagtacgta
    39841 accgtgttgg tcgtggtact tcatctggta acggaaaaac tgccggacgc ggacaaaaag
    39901 gacaaaaagc tcgttcaggc ggcggtgtgc gtttaggatt tgaaggggga caaacaccat
    39961 tgttccgtcg tttaccaaaa cgaggattca caaacgtgaa ccgtaaagac tatgcagtca
    40021 ttaatcttga tgtattgaac cgctttgaag acggagctga agtaactcct gttgctttag
    40081 ttgaagctgg aatcgtgaaa aacgaaaaag ctggaatcaa agtattagct aatggggaac
    40141 taaacaagaa attaactgtg aaagcagcaa aattctctaa agcagctcaa gaagcaattg
    40201 aagcggctgg tggatcaatc gaggtgatct aatgtttaga ctattaaaag atgcttttaa
    40261 agtaaaagac attagatcga aaatcctatt tactgtattt gtgttgtttg tatttcgcct
    40321 aggtgcacat attactgtac caggcgtaaa tgctgcttca ttgcaaaacc tttcttcttt
    40381 gccatttttc aacatgttga acttggtaag tggtagtgcg atgcaaaact tttccatttt
    40441 ctcaatgggg gtttcaccat acattactgc ttctatcgtc attcagcttc ttcaaatgga
    40501 catcgtgcct cgatttgttg aatggtcgaa acaaggggaa gttggtcgta aaaaactgaa
    40561 tcaagcgact cgatatctga cattagtatt ggcatttatc caatctgtcg gtatcactgc
    40621 tggattcaat caatacgctc aattaggttt cgtgaataat cctaacgtag caacttacgt
    40681 aacgatcggt ttgattctaa cagccggtac gatgtttgtt acttggctag gagaacagat
    40741 cacagaaaaa ggtatcggaa atggcgtatc aatgatcatt tttgcaggta tcatttctag
    40801 attgcctgaa agtatcagag aaatttatga agattacttt ataaatattg atccttcaga
    40861 catatggaaa tctgctattt ttgtggccat tttactagta gcggtcttgt tgatcgtgac
    40921 attcgtaaca tttttccaac aagctgaacg caagatcccg atccaatata cgaaacgcgt
    40981 agctggcgcg cctacaagca gctatctgcc gttgaaagta aatgcagctg gagttattcc
    41041 agttatcttt gctagttcgt ttattgctac gccaaatgct gttttacaag cattgagcgg
    41101 tagattcggt tctgaaaact ggtacaaagt gatgatggag atctttaact ataatacagc
    41161 acctggtgct acgatttata cgattctgat cgttgccttc actttcttct acgcctttgt
    41221 tcaagtaaac cctgagaaat tagcggaaaa cttgcaaaag caaggaagtt atataccaag
    41281 cgtacgccct ggtaagggga ctgaagagta tgtctcaaga ttactgatgc gtttaagtac
    41341 agttggtgca gtcttccttg gtttagtggc attgcttcca atcattgctc aaatggtttg
    41401 ggatcttcca caatcaatcg gattaggcgg tacaagctta ctgatcgtta tcggtgtagc
    41461 tttagaaact gcgaaacagt tagaaggctt aatgctgaaa cgtaaatatg ttggattcat
    41521 tgagtaagaa agcgtgttga ggtttcccct caacacacgt tctcacacat ttattaggag
    41581 ggaaaacaat gaacctcatt ttaatggggc tgccaggcgc aggtaaagga acacaagctg
    41641 aaaaaatcat tgatgcttat ggtattccgc acatctcaac aggagatatg ttccgcgcag
    41701 cgatgcaaaa cgaaacagct cttggcctgg aagcaaagtc ttatatggat aaaggtgcat
    41761 tagttcctga tgaagtaaca aatggaatcg tgaaagagcg actagctgaa ccagatacag
    41821 agaaaggctt cttattagat ggctttccac gtacgctaga tcaagcaaaa gctctggacg
    41881 caatgctaaa agatttgaat aaaaaaattg atgctgtcat cgatatccat gttggtgaag
    41941 aaatcttggt cgaacgtcta gccggccgat ttatctgcag caattgtgga gcaacttacc
    42001 ataaaatttt caaccctaca aaagttgaag acacatgtga tcgttgcggt gggcatgaat
    42061 tctatcaaag agaagatgat aaacctgaga cggttaaaaa tcgtctggct atcaacatca
    42121 aaaatagtga accaatcttg gcttattata aagaacaagg tttgctaaac actattgatg
    42181 gtgctcgtga aatcgatgct gtatttgcag atgtacaaaa aatcattgaa aaatagcacg
    42241 attgcccgta gccgacttgc attatgcagt tactcttgtc aagttcaata aattatgata
    42301 gaatatgtca gtccgacttc tagagaatcc tctagatgat ccattttgag aattttgagg
    42361 tgggaactgc agtttccgcc attttatcgt gtgaaaatgc gaaacaagta caaggaggta
    42421 ctgtgcgtgg caaaagaaga tatgattgaa gtcgaaggta cagtcgtcga aactttgccg
    42481 aatgcaatgt ttaaagttga actagaaaac ggacaccaag ttcttgctac tgtttcaggt
    42541 aaaattcgta tgcattacat ccgtatctta cctggtgaca aagtaacggt tgaattgtct
    42601 ccatacgatc ttaaccgtgg ccgtattact tatcgcttta aataattgta ctccgtaaaa
    42661 ctacaggagg tattatcatg aaagtaagac catcagtaaa accaatgtgc gaacattgta
    42721 aagtaattcg tcgtaaagga cgcgttatgg tgatttgccc agcaaatcca aaacataaac
    42781 aacgtcaagg ataattggag gtgctataaa tatggctcgt attgcaggag tagatattcc
    42841 tcgtgataaa cgtgtagtag tttcacttac ttatatctat ggtattggta acactactgc
    42901 taaaaagatc ttagctgatg ctggtgtatc tgaagatgtt cgcgttcgtg aattaacgaa
    42961 tgaacaaaca gatgctatcc gtgcagaagt agataaatta aaagttgaag gggatcttcg
    43021 tcgtgaagtg aacttgaaca tcaaacgttt gatggaaatc ggttcttacc gtggaatccg
    43081 tcatcgccgt ggattgcctg ttcgtggaca aaacacgaaa aacaatgcac gtactcgtaa
    43141 aggcccagct cgcgctatcg ctggcaagaa aaaataattc ttaagtgaag gaggttaaaa
    43201 cttcatggca gcaaaaaaag tgaatcgtaa acgccgtgtg aagaagaata tagaagcagg
    43261 tattgcacat atccattcta cattcaataa cactatcatc atgatcactg atactcacgg
    43321 aaatgcatta gcgtggtcat cagctggttc attaggtttc aaaggaagca aaaaatcaac
    43381 tccttttgcc gctcaaatgg cagcagaaac tgctacaaaa gcagcaatgg aacacggatt
    43441 aaagactgtt gaagtaacag ttaaaggacc tggttctgga cgtgaagcag caattcgttc
    43501 attacaagct acaggtttag aagtgactgc aattcgtgac gtgactccag ttcctcataa
    43561 tggtgcccgc cctccaaaac gccgtcgtgt ttaatgagtg ccgctcattt tgagttttat
    43621 cagtaacgtc attgaacaag acacttttcg ttttgaaagg ggtaaagata agaatgattg
    43681 agttcgaaaa accaagaatc gcaaaaattg atgaagaaaa agattatggc aagttcatcg
    43741 ttgaacctct tgaaagaggg tatgggacta ctttagggaa ttccctacgt cgtattttat
    43801 tatcttcttt gccaggtgct gccatcacta atattcaaat cgatggtgta ctgcatgaat
    43861 tctctaccat caaaggtgtt agagaagacg tcactcaaat catcttgaac attaaaggct
    43921 tagcgctgaa aatgtatggc caagaagaaa aaacccttga aatcgatatt accggacctg
    43981 ctacagtaac tgctggcgat attatcgttg atagtgatgt agaaattctt aacaaagata
    44041 tgtacatctg tagtgtctct gaaggagcta cgttccatgc tcgcttaaca gtgaaacctg
    44101 gacgtggtta tgttcaagca gatgaaaata aaaaagaaga tatgccaatt ggcgttcttc
    44161 cagtcgattc aatctacaca cctgttcgtc gtgtgaacta tcaagttgag aacacacgtg
    44221 ttggacaccg tgaagatttt gataaattga cgatggagat ttggacagat ggttcaattg
    44281 aaccgttgga agcaatgagt ttgtctgcga aaatcatgac tgaacatttg gatatttttg
    44341 tgaacttaac tgatgaagca aaaaatgctg aaatcatggt cgaaaaagaa gaaacacaaa
    44401 aagaaaaaat gttggaaatg acgatcgaag agttagactt gtctgttcgc tcttacaatt
    44461 gtttgaaacg tgcaggaatc aatactgtac aagaacttac aaataaatct gagcctgaaa
    44521 tgatcaaagt ccgtaactta ggacgtaaat cattagaaga agttaaagca aaattgcacg
    44581 acttaggttt aggtttacgt aaagaagatt aaaacctttt acgaaggagg gaaaccaacg
    44641 tgagttatcg taaactagga cgcacatcta gccaacgtaa agcgatgttg cgtgatttaa
    44701 caactgattt attaatcaac gaacgtattg tgactactga agctcgtgcg aaagaagttc
    44761 gctcaactgc agaaaaaatg atcactttag gtaaacgcgg agatttgcat gcacgtcgtc
    44821 aagctgcggc tttcgtacgt aatgaagttg ccagcgtgcg tgaagaaaat gaggacatcg
    44881 tcattgaatc agctttacaa aaactgttca acgatattgc acctcgttac gcagaacgtc
    44941 aaggcggata cacacgcatt ttgaagacag aaccaagacg cggtgatggt gcaccaatgg
    45001 ttgtcttaga acttgtctaa aatcacacta ataacatcac tttatagatg attcttttag
    45061 agtgttatga tgttggaaga tatttttccg agtctagctc agcgctgctc ctcaagcgaa
    45121 tatcgcttgg ggagtaagca ctcatcataa agtgtttttt tataccttca attacctgtc
    45181 atgacagata tgtttgaagg cataaaaaaa cattttaagc gagaaataat tgaaaatttt
    45241 gtcttttttt gatagtgtta gtaggaactt taggatgaag aaggagtaat catggagcca
    45301 attattgaat tagaaaagat aaattataaa taccagccag atgatcttcg ccccgcattg
    45361 aaagatgtct cctttacaat tgataaaggt gagtggatcg ctattattgg acacaatggt
    45421 tcaggaaaat cgacacttgc caaaacgatc aatggattgc ttctgccaga atcagggatc
    45481 gtgaaagtgg gaaatcaaat attggatgaa gaaaatattt ggacgatccg acagatggtt
    45541 ggaatggttt ttcaaaatcc agacaatcag tttgttggtt cgacagtaga agatgacgtt
    45601 gcttttggtc ttgaaaatca agggattccc agagaagaaa tgctagtgcg tgtgaaagat
    45661 gcacttgaga aagttagaat ggctgagttt gcttcacgcg agcctgctcg tttatcagga
    45721 gggcaaaaac aacgtgttgc cattgccggc gtggttgcct tacgacctga tatcattatt
    45781 ttagatgaag caaccagtat gctagatcca gaaggtcgtg aagaagtgat ctctacgatc
    45841 aagaagatca aagaagaaag tcaattgacc gttatatcta tcactcatga tattgatgaa
    45901 gcagctaatg ccaatcggat actagtgatg agacaaggcg aacttgtccg tgaaggtaca
    45961 ccaaaagaaa ttttttctgc tggtcctgaa ctgatcgact taggtctaga tctaccattt
    46021 cctgaaaaac tgaagagcgc actgaaagaa cgtggggttg atgtgcccag tgaatatatg
    46081 acagaagaaa ggatggtgga ttggttatgg acatccgttt tgaacaagta gacttcactt
    46141 atcagccgaa tacaccattt gaacaaagag ccttatttga catcaatatg acgatcaaag
    46201 aaaacagtta tactgcatta gtagggcata ccggaagcgg gaaatcaacc ttacttcagc
    46261 atttgaatgc gcttgttaag ccaacaagcg gtacagtcca tattggagaa cgagatattc
    46321 agccggatac ggataataag aatttaaagc ccatccgaaa aaaagttggc atcgtctttc
    46381 agtttccaga agcacagcta ttcgaagaaa cggtagcaaa agacattgct ttcggtccta
    46441 aaaactttgg tgtcagcgaa gaagaagcac tagtcctagc aaaagaaaca ttagaacaag
    46501 ttgggctgga tgaaagctat ttggaacgtt cgccatttga actttcagga gggcaaatgc
    46561 gtagagtagc aatcgctggt gtgcttgcca tgaggccgga agtacttgta ttggatgaac
    46621 caacggcagg acttgatccc cagggtagaa aagaaatgat ggagatgttt tggcgtcttc
    46681 ataaggaaca gcagatcact attgtattag ttacgcattt aatggatgat gtagccaatt
    46741 ttgcagatta tgtctatgtg ttggaaaagg gacgaatcgt taatagcggg gagccgcaag
    46801 aggtctttca aaacattgaa tggctaaaag aaaaacaact aggcgtacct actgccaccg
    46861 agtttgcaga agaattaatg gctaaaggga tgaactttgc taccttgcct ttgacagctg
    46921 aagaattagc agacgctatc gtccattatg ctggaggaaa tgcccatgat gaataaattg
    46981 atatttggtc gttatatgcc tggagattcc ttgatccatc gattggatcc ccgagctaaa
    47041 ttattagcca gtttttattt tatcggaatc atttttttag caaataattg ggaaacgtac
    47101 cttttgttgg catttttcac attactttct gtcttcttgt caaaagtaga tttgaaattt
    47161 tttatccgag gcattcgccc attgatctgg ctaatcctct tcactgtagc tttgcaggtc
    47221 ttgtttacaa gtggcggaga aacttattgg agttggggtg ttttccatat cactgaattt
    47281 ggtgtgatga acggcctctt tattttctgt cgattcgtgt tgatcatttt tatgtcgact
    47341 ctactcacgc ttactacacc gcctttggaa ttatcggatg cgattgagta cttgcttcgt
    47401 ccattgaaag ccgtacggtt cccagtccac gaaatttcat tgatgctgtc gattgctctt
    47461 cgttttgtgc ctacattgat ggacgaaaca gaaaagatca tgaacgctca gcgcgctcgc
    47521 ggtgttgatt ttggtgaagg aaacctagtt cagaaaatga aagcagtcat tcctttgctg
    47581 attcctttat ttgtcagcag cttcaaccgt gcggaagatc ttgcgactgc tatggaagca
    47641 agaggctaca aaggtgggga aggaagaacg aaatatcgtg tattgcattg gcataataga
    47701 gacacgatgg ttatcattgc ttttgtatta ttaactgtgc tactgatctt tttgagagga
    47761 taaacgttca gtgggccgct atctattctt ttcttgctga tctgtttgca agaaaggatg
    47821 cgagcagtgt atgacaagaa atattagggt ctgtggcaga ctaaggtcac aggcctcttt
    47881 cttttatctg aaaagaaaga acgaaaaaat tttataaaaa aatcattatt ccagataacc
    47941 ttttaggaac aaaggagaga aaaacatggt acgttacaaa gcaatcattg catacgacgg
    48001 aacaaatttt aatggattcc aaaaacagcc aaatgggcgt actgttcaag aagaagtaga
    48061 aaaaacatta caaaaaatgg caaatggaaa agaaatcacg gtctttggtt cgggaaggac
    48121 agatgccgga gtacatgcga ttgggcaagt aatccatttt gattatccag aggagcgtcc
    48181 gttagaacga atgcgtttcg cgctagatac ccagtcacca gaggatattg ctgtaagaca
    48241 agtagaaatt gtttcagaag acttccacgc aagatatttg gtcaaagaaa agacgtatca
    48301 atttcgagtg gatataggga aaccacgcag tccttttcga cgccattatg caagttattt
    48361 tccttaccct cttgatttgg aaaaaattca gcgggcgctt cctgatttat tagggactca
    48421 cgatttcacc tctttttgtg catcaggaag ctcgatagag gataaagtac gtacgatcta
    48481 cgaggctaag atggaggtaa atgaaacaaa agacgaattg ttgtttacat ttaggggaaa
    48541 tggcttcttg tataagatga tacggatctt agtaggaacg cttttgaaaa ttggcaatgg
    48601 ccgattgcct gaagatagta tcccagatat tattgcgaaa aaagatcgta atgcagcagg
    48661 acctacagca catcctgaag gactttattt gtatgaagtg aaatatgaat aagaggttgt
    48721 gacagaagtg gtcagcttca agaaataaga aggaatttac gaaaattact tctcaaaatt
    48781 ttgcaaaatt tcggcttatt ttcgaaggag ctacttctgg aacaccgtga atcagaggtt
    48841 gtgaaaaagc tgccctgcat caagtaataa gaacaaccaa acaaaaatag ctcgtcatat
    48901 ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt ttaaggaaga
    48961 ggttgtgaaa tcagcgtttt gacctgagta ttagagaaac aaacggaaaa atagttcctc
    49021 atattttgcc gtttgttgag ctaataccga aggtcagctg attgaacacc gtttaagaaa
    49081 gaggtcgtga gcctgtcatt taactctgag tcacaagtaa caatttcaaa aaacagcttc
    49141 tcatctttga ggaatatcgt gacttgtgga cgaggcgttg ataagtgaat catctttttg
    49201 ttacctattt tttctgtaac caaaaaacga tggaacgttg ttttgattgc ccttaatgag
    49261 gacagtcgga acaggtttgt cattttta
//
LOCUS       DAXWOO010000005        42319 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.005, whole genome shotgun sequence.
ACCESSION   DAXWOO010000005 DAXWOO010000000
VERSION     DAXWOO010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42319
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.005"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(<1..322)
                     /locus_tag="ACGE70_000218"
     CDS             complement(<1..322)
                     /locus_tag="ACGE70_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFF8691891.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQ"
     gene            complement(322..552)
                     /locus_tag="ACGE70_000219"
     CDS             complement(322..552)
                     /locus_tag="ACGE70_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFF8691892.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            839..1177
                     /locus_tag="ACGE70_000220"
     CDS             839..1177
                     /locus_tag="ACGE70_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8691893.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(1382..1944)
                     /locus_tag="ACGE70_000221"
                     /pseudo
     CDS             complement(1382..1944)
                     /locus_tag="ACGE70_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - obsolete protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(2676..3257)
                     /locus_tag="ACGE70_000222"
     CDS             complement(2676..3257)
                     /locus_tag="ACGE70_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFF8691894.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(3440..4066)
                     /locus_tag="ACGE70_000223"
     CDS             complement(3440..4066)
                     /locus_tag="ACGE70_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFF8691895.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(4088..5419)
                     /locus_tag="ACGE70_000224"
     CDS             complement(4088..5419)
                     /locus_tag="ACGE70_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFF8691896.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(5574..5975)
                     /locus_tag="ACGE70_000225"
     CDS             complement(5574..5975)
                     /locus_tag="ACGE70_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFF8691897.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(6061..6267)
                     /locus_tag="ACGE70_000226"
     CDS             complement(6061..6267)
                     /locus_tag="ACGE70_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8691898.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(6362..7864)
                     /locus_tag="ACGE70_000227"
     CDS             complement(6362..7864)
                     /locus_tag="ACGE70_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFF8691899.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7882..8337)
                     /gene="argR"
                     /locus_tag="ACGE70_000228"
     CDS             complement(7882..8337)
                     /gene="argR"
                     /locus_tag="ACGE70_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFF8691900.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(8435..9052)
                     /locus_tag="ACGE70_000229"
     CDS             complement(8435..9052)
                     /locus_tag="ACGE70_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFF8691901.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            9273..10646
                     /locus_tag="ACGE70_000230"
     CDS             9273..10646
                     /locus_tag="ACGE70_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFF8691902.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10721..11833
                     /locus_tag="ACGE70_000231"
     CDS             10721..11833
                     /locus_tag="ACGE70_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFF8691903.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(11905..13542)
                     /locus_tag="ACGE70_000232"
     CDS             complement(11905..13542)
                     /locus_tag="ACGE70_000232"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFF8691904.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAVVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(13710..14318)
                     /locus_tag="ACGE70_000233"
     CDS             complement(13710..14318)
                     /locus_tag="ACGE70_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFF8691905.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(14702..15853)
                     /gene="queG"
                     /locus_tag="ACGE70_000234"
     CDS             complement(14702..15853)
                     /gene="queG"
                     /locus_tag="ACGE70_000234"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFF8691906.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(15872..16624)
                     /locus_tag="ACGE70_000235"
     CDS             complement(15872..16624)
                     /locus_tag="ACGE70_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8691907.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(16765..17715)
                     /locus_tag="ACGE70_000236"
     CDS             complement(16765..17715)
                     /locus_tag="ACGE70_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFF8691908.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(17716..18555)
                     /locus_tag="ACGE70_000237"
     CDS             complement(17716..18555)
                     /locus_tag="ACGE70_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFF8691909.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(18552..19298)
                     /locus_tag="ACGE70_000238"
     CDS             complement(18552..19298)
                     /locus_tag="ACGE70_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFF8691910.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            19536..20906
                     /locus_tag="ACGE70_000239"
     CDS             19536..20906
                     /locus_tag="ACGE70_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFF8691911.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(20961..21857)
                     /locus_tag="ACGE70_000240"
     CDS             complement(20961..21857)
                     /locus_tag="ACGE70_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFF8691912.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(21971..22141)
                     /locus_tag="ACGE70_000241"
     CDS             complement(21971..22141)
                     /locus_tag="ACGE70_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8691913.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            complement(22138..22815)
                     /locus_tag="ACGE70_000242"
     CDS             complement(22138..22815)
                     /locus_tag="ACGE70_000242"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFF8691914.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(22906..23811)
                     /locus_tag="ACGE70_000243"
     CDS             complement(22906..23811)
                     /locus_tag="ACGE70_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFF8691915.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            23986..25440
                     /locus_tag="ACGE70_000244"
     CDS             23986..25440
                     /locus_tag="ACGE70_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFF8691916.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPSH"
     gene            25456..26487
                     /locus_tag="ACGE70_000245"
     CDS             25456..26487
                     /locus_tag="ACGE70_000245"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFF8691917.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGHFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            26494..27681
                     /locus_tag="ACGE70_000246"
     CDS             26494..27681
                     /locus_tag="ACGE70_000246"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFF8691918.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            27837..29219
                     /locus_tag="ACGE70_000247"
     CDS             27837..29219
                     /locus_tag="ACGE70_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFF8691919.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            29191..29577
                     /locus_tag="ACGE70_000248"
     CDS             29191..29577
                     /locus_tag="ACGE70_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8691920.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(29777..30445)
                     /locus_tag="ACGE70_000249"
     CDS             complement(29777..30445)
                     /locus_tag="ACGE70_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFF8691921.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(30634..31431)
                     /locus_tag="ACGE70_000250"
                     /pseudo
     CDS             complement(30634..31431)
                     /locus_tag="ACGE70_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(31712..33091)
                     /locus_tag="ACGE70_000251"
     CDS             complement(31712..33091)
                     /locus_tag="ACGE70_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFF8691922.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            complement(33266..34435)
                     /locus_tag="ACGE70_000252"
     CDS             complement(33266..34435)
                     /locus_tag="ACGE70_000252"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFF8691923.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            complement(34410..35561)
                     /locus_tag="ACGE70_000253"
     CDS             complement(34410..35561)
                     /locus_tag="ACGE70_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFF8691924.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            complement(35521..36882)
                     /locus_tag="ACGE70_000254"
     CDS             complement(35521..36882)
                     /locus_tag="ACGE70_000254"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFF8691925.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            complement(36879..37880)
                     /locus_tag="ACGE70_000255"
     CDS             complement(36879..37880)
                     /locus_tag="ACGE70_000255"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFF8691926.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            complement(37896..38591)
                     /gene="dapD"
                     /locus_tag="ACGE70_000256"
     CDS             complement(37896..38591)
                     /gene="dapD"
                     /locus_tag="ACGE70_000256"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFF8691927.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            complement(38597..39313)
                     /gene="dapB"
                     /locus_tag="ACGE70_000257"
     CDS             complement(38597..39313)
                     /gene="dapB"
                     /locus_tag="ACGE70_000257"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFF8691928.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            complement(39310..40191)
                     /gene="dapA"
                     /locus_tag="ACGE70_000258"
     CDS             complement(39310..40191)
                     /gene="dapA"
                     /locus_tag="ACGE70_000258"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFF8691929.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            complement(40194..40994)
                     /gene="dapF"
                     /locus_tag="ACGE70_000259"
     CDS             complement(40194..40994)
                     /gene="dapF"
                     /locus_tag="ACGE70_000259"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFF8691930.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            complement(40981..42240)
                     /gene="lysA"
                     /locus_tag="ACGE70_000260"
     CDS             complement(40981..42240)
                     /gene="lysA"
                     /locus_tag="ACGE70_000260"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFF8691931.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
BASE COUNT        11749 a         8858 c         7604 g        14108 t
ORIGIN      
        1 tctgatcaat tttagccatg tcttgcaatg gtaagttttc tatttttttg agttttcgtt
       61 ccttaaaatc aagagatttt aattgtgtaa taattacttg tccttttata tctagctctt
      121 caggtaatga gtaacgtgtc ggtaaattct ttatagtgga agtaattgga catatgaccg
      181 aaaacattgt tttacggtta aattcataac gagagacgac tagtcctggt cgtctttttt
      241 gaatttcttt tcctacggag ggatcaaaat caatccatac aatatcacct tttttaggaa
      301 tataaattct ttcttctgac actataaaat ttctctccct tcaggagtta aatcttccca
      361 ttcgtctttc tcataatact cggcctcttc cccaccacta aatggatctt caatttttgg
      421 aactaacgta attgttccat catcagaata cacaacgata tattcttggt tttctgaagg
      481 tttctttccg ttatcagaag gtaaagttac cactacagaa cttccttgta atctagattt
      541 tgtaacaagc attatcatca ctcccttaat aaaagtatac tatataacac taagtaacgc
      601 aaaattcccc tttgtaaact atctagaaaa ttgttttgtt taaagtatat cttattggat
      661 ataaatagaa ataaattgta gccagttcat tttttcttta actaaaaaaa taatccaaga
      721 aacattgaac ctatggatcc aaaagctacc caaatactat agctaacccc ccgctacctt
      781 caattttgca gataaaacgc tttgggagaa aatttcaaag acaacaggcc atcctatcat
      841 gcccgattat aaacaaaaag ctggctattc cttttgctct gacaaatttc aattgggaaa
      901 gttcacgaaa ctagcaggtg aagaagtttc aactgttcgg atcaaagaat gcccgcttca
      961 aatagaaaca ttggttcaac gaatttcaac tagacaagac tttgctattg tagaacgtga
     1021 gatcgtagct atttttgtag aagaagggat tttacatgat ccaactcata tcaatgtgga
     1081 taaatggcaa cctcttatct ataaattcag agaatataca actgccaacc agtcgttaac
     1141 tttgaatttt agattccaag aattcaaaga gtcataatga taaacatcgt taggtataaa
     1201 taaaaaagac tcgcagcacg agtaaaaaac aaatagaagc tatacattag aaaggtcgac
     1261 tgtgacccta tagatgcagt atatgcagct tatgagtatt tcttctgttc tctttgtttt
     1321 tgggatctgt gctgaagtct ttttcgtttt ttgccgctct ttctttgaaa aatcgattat
     1381 gttatacctt ctccattact aattcaggca tctgctgagt aatgattttt cgcgcaaaag
     1441 aaatatctgt tacccaaggt tcgatttgtt cttcagtcaa gacaagcggc attcggtcat
     1501 ggattggtgc gactgtgcgg ttaggttcag tagtcattat gattgattct gccatttttt
     1561 catcctgatg gctacgataa aaaccgccaa tatacactat tttttcctta tttgaaaaca
     1621 taaatttgtc tttttcttca ttccactcat tgggaaaaca caacgatttt gcaaaaatgg
     1681 ttgatgaaag gtcttttttt cttctactgt ctctgcacgg gcattaatca tcaattgtcc
     1741 ttttttaaat ccggtaaatc cccacttcat gatttccgga gtgaggatgt tttcttcctc
     1801 ttttcttaac gtcacaacaa gatttgacgg aaaaacttct ccagctgcta attctgctgc
     1861 ctgcttcggt tgtttcgtct gggcttctcg ataataattc tgaaggagct ctgactctag
     1921 atcaaaaaaa taacgaccac acatcctaat ccctcccaat ctttttttct atcatacact
     1981 gcaacaagaa gttcgcacca acaaaacgct ttcaattttt gtagaactaa aaaagttgtg
     2041 acaagtgaca aactctccag tgaatggtat tctcctgcca actcctcggt cacaagtcac
     2101 aatattcaac aaacatgaga agctgctttt tgaaattgtt ccttgtgact cggagtgaaa
     2161 cggcaggctt acgacctctg ggtaaacggt gttcaaaagc cagtttctcg gtattaagcc
     2221 aaatttatgc aaaaatgtag aaagctattt tcacaaaatt tttcttaata ctcgaaacta
     2281 agcgactttt tcacaacctc ttccttaaac ggtgttcaaa agcttgcatc tgcggtaata
     2341 agcccaacca aacgaaaaat atgagcagct atttttattt ggctgttctt attacttgat
     2401 gcagggcagc tttttcacaa cctctccact aaacgatgtt caatcagcta accttcggta
     2461 ttagctcaaa aaacggcaaa atatgaggaa ctattttgcc gtttgtttct ctaatactca
     2521 ggtcaaagcg ctgatttcac aacctctaat acacggtgtt ccagaagtag cttcttcgaa
     2581 aaataagccg aaatttcaca aaaatttgag aagcaatttt cgtaaattcc ttcttatttc
     2641 ttgaagctga ccacttctgt cacaacctct aattattaaa gaaccttttg cgaagttaac
     2701 aatctttctt tgatcaattg aaaattctgc ctaagtagtg atagatcctg tgtgactttc
     2761 aacaataaga tacttccctg gatttcagca atcaacgctt tggcttgacg gtctgcttct
     2821 tcatcggaca gtttttcttc agaaagtgcc aggatatttt gtttcgtata cttgacccat
     2881 tgattgacta attctagtag caacagacgg tattcttcat ccactgtact caactcaata
     2941 atcaaattac ctacaggaca gccatgataa gtgatctgtg tctcatgatc agcgaaaatc
     3001 cagtccaaca tttcttctaa tgcttcttct ggctgttttc cagcatttaa gatgtcttcg
     3061 aacaattctg tctgccatac gtccatatgc gcatggatca ccgctagaca aacttctttt
     3121 tttgaagaaa agtaataata aaattgacct tttcctgtat cagaagctgc tagaatatct
     3181 cggattgacg tttcatgata gcctttttta taaataagat ctttggtcgc ttccaagatc
     3241 ttttcctttg ttttcaatga tattctcctt tttcatttct catagtccta tattatacag
     3301 taaattcatc attttttctt cttaatcgtt taaaaaagac tgtgccaaat tatctgacac
     3361 agccctcact ataaaactgt tctcatatag ctagatgttc attgattcaa gaactgcgag
     3421 gacaaaagca attcctatct tatgatagtt gctctaatac ttcctctgtt gtcatcactt
     3481 gatctgcaat aaagccaaag ttcgtcaacg ctgcatccat tgcttcttgg ccaggagcac
     3541 caattgcatc tgtcgctacc acaacattga atccactctc taccaattca cgcatatggg
     3601 aatcgctaca caagtttccg ttcattccac caagaattac tgtgtcgatt ccattttttc
     3661 tcaattggag cgccaaatca ttgctttctg gtccaaagat tttatgagga gagctgatga
     3721 tcgtattttc atccaaataa ggtttcaatt cttcgataaa atcggctcct gaaccttctt
     3781 ccactgattc atattgactt tttctagcaa acatctgctg ctcaagcatc atatgttctc
     3841 ctgcaccgcc aaattgccat ttatggtcat gtgggtaaaa ataatgagga gaaataaaga
     3901 gtttgtatcc tttttctttt gcttgtttta ccatttcaat catattttcg atcgtattgt
     3961 atttttctaa gatatctttt gtcaaaccaa agccaccgcc tgctggtttc aagaactcga
     4021 cttgcgggtc agtgagaact actgctgtat ttttattatt gaacatggtg gattctccat
     4081 tcgttattta ttttaataca tcatttagag cttcactcat tgtcggatga gaatagattt
     4141 gatctctcaa taacgtataa ggaagttttc cattcatagc tagcgaaatc aagttgatga
     4201 cttcatgaga atcttccgca tagatacttg cccctagaat caagtcagtt tcagggtcga
     4261 ctaaaacttt gaatacacct ttaggatctt ctaatacttg tgctttaggg acaccgcttg
     4321 tcatatactt gaataatttg taattgacgc cttgtgcttt ggcttgtttt tctgttaaac
     4381 ctacattgga taaagtcgga gtgataaata cactgtaagg cacagctttc cggttattcg
     4441 ttgttcgttt attctcgccc ttcaactgat ccataatgat tcgataatca tctagagaga
     4501 tataagtgaa ctgcaatccg cctttgacgt ctccaatcgc ccaaatgttg tctgcatctg
     4561 tacgaaggta ttcatccaca gcgattgctc cgcgatctgt tagtttaaca tctgtatttt
     4621 ctaatccaag tgatgctgta cttggttttc ttccagttgc agccaaaact tttgacgctt
     4681 ggattgttac ttcttggcca tcttctgtaa aggtgacggc agctgttgat tcttggtctg
     4741 ctacttgatc tacagataca cctagatgga attcgattcc cgcatttgtc atgtcttcta
     4801 aaatcatttg cgcgatatct tcatcttcac gtggaagaaa ttcagaatgg gcatcaagaa
     4861 cgacaacttt agatccgtat tcattgaaca ttgatgcaaa ttccaagcca atatatccag
     4921 ccccgatgat cactaatttt tccggtaatt gttcctgatc catcgcttgc gtagaatcaa
     4981 tcaagtagcg acttgtattc aatcctttga ttggtaaaat gattggttca gaaccagtat
     5041 tgatgaaaat ccgttcgcct tcaacaatca ctttttcacc attattcgtc acttcgattt
     5101 gatggttaga aagaaaacgt gctgtaccgt ccatcactgt cccagttgcc tcatcgctga
     5161 tcatatgata atttttggcg cgaagcatgc ccgtcaattt ttctttccga gcaacagcct
     5221 ctgtaaagtc gattcctctt tctccattga aaatcaatga tttagaagga atacagccga
     5281 tgttgataca tgttccgcca tacatacgtg ttgacttttc aacgactaaa accgattcac
     5341 cttttgaagc taacgccttc gctaaagtct taccgccctt tccaaaaccg atcacaatat
     5401 tttgatattt ttccatcatt tttccctcgc ttctttttta cctaccgatt ggtacaaaaa
     5461 tatgataacc gataattgtc tattctccaa atcatctgct ttatttgtca aaaaaaacca
     5521 gacaaaaagc ggtcctttca cgtttttagt ctggttcgat ttcctttatt cttctaaaca
     5581 atcgttactt ctacatcgat atttcctcta gttgctttgg agtaagggca gaattcatgc
     5641 gcatcttctg caattttttg ggcttcttct tcgctgatgc catcgatatg cacttctaat
     5701 actacaccga tctgatagcc gccatcatct aaattataaa gagagacacg tgcttttact
     5761 gtggactctg ctgtcattcc tgcttctttg atcatatgtt gtaccgcccc attgaaacaa
     5821 gaagcataac ctgctgcaaa taattgttct ggatttgttc cttctgcatg gattcctgga
     5881 ggagtgattt tcatatgcat cgagccattt ggtgcctcga cttctccatt gcgcccgcca
     5941 tggttgatca ttgctgtttc gtatgctttt ttcattgtac tgctcctttc gattgaccat
     6001 ttgctacttg gctataatta tagtgtatga caagtaaggc gctttctaca aaaattctgc
     6061 ttattcgaat accagaatgc cgtcttctac tatttttcca aaaaacaaaa aaatactact
     6121 cgtgaacaac agcgtcatat ttgttccaaa ataaatagca atcttttcta cataatcttt
     6181 tatatgattg attttaagat acatatatac cagaaagttg accccataaa ccaatactgt
     6241 taaaaaaata accatattcg ttaccatttt ttcctccttt tgctggtctc cctaccagca
     6301 ctcgatttaa ccataccact ttttttgccg tccaagcaac tagcctttca aaaagactgg
     6361 tttattctgc tgttaatgat tcaagatcag aaaaatgtcc gaataatttt gctaatttta
     6421 ctaacgccaa cttgttcttt ccgaattcat cattttctat ctgtaatact agtaacggct
     6481 catgtaagct taaacggatc aaaaaccagc cgtttccgta tggctcttct agatcatatc
     6541 gaataccttc ttcattttcc gggtcgaagt tcatcccttt ttcttcttcc aacagtcgct
     6601 tcatttcttc taatatcttg tttccttgtt cgcgtggttc atcgctgaac aattttaatc
     6661 ggatttcttg tacttctacg ggctgtttca actctttgat ttgttcatct aatgtgatac
     6721 cttttctttt taattctggc aaaagcatca ggatctttgc gatgacatat gcaccatcat
     6781 ccagaaaata gttttccttg aatgcagcgt gtccgcttgt ttcgatggct agctgtgtat
     6841 ctttcccaag acggttaagt tctaatgctt tattgattac atttcgatag ccggaaatat
     6901 aacgaacttg gatgcctcct ttttcttcaa tgaatctttg tagatgagaa gaagtagggg
     6961 aattcgtcac gattcttgtt cctggatgct cttttaatac gattgtactt aatactgcga
     7021 tcaaattatt tcgattgatc actgttccag aagcactgac aactgcagaa cgatccacat
     7081 ctgtatcgaa aatgatccca agatctgctt catttgctaa aacagcctgc ttgatacttt
     7141 ccatcgcttc tttattatca ggatttggga tatgattagg aaatcggcca tctggttcca
     7201 aaaattggga acctgttgaa tccgcaccta agctattcaa tactttttca gcaaagaatc
     7261 caccagcacc atttccggca tcaacaatga ttttccaacc atttagtggt ttgctttcta
     7321 gtgttccaat ccccttttta atttttgtga ctaaatcttg cgcataaggt gtcaaaaggt
     7381 ctgctttttc tacgcttcca gtttgttcgc tttttatttt ttgctgctgt gataggataa
     7441 aagcaatatc ttctttttct gctcctcctt ctgaggtaaa gaattttatt ccattataat
     7501 aatagggaag atggctagca gtcagcataa ccgcagcatc gcacgagaac tgttcaaatt
     7561 gagtggacat aaacatagca ggcgtagtag ctaattcgaa atcgatgaca tgtatccctt
     7621 gttctttcag actttggatc aacgcctctt tcagtgctgg tccgcttatc ctactgtcat
     7681 gaccaatccc aatcgtccat tttttatttg gatcggttga tttcttcttt ttctttatcc
     7741 agtttgctag tccccagccg atctttttta ccatgtctgt cgtcaagttt acttcatatc
     7801 cttcaactgg taatgcgatt cctcgaatat ccgatccatt ttgcagcgct ttcatatctt
     7861 tcatataaaa gtcctcctca tttattccct cattttttcc agcttttctg caaatcgctg
     7921 tgcttcttct tctgagcggc aaataataat gaatgtatct acaccggcca tcgttccgac
     7981 aacttcaggg taagccactt catctagcca attcgttacc acatcggcac catttcgttc
     8041 agtcaatacg ataaccatga actgaacaga ctggatacgc aaaacctcgc gtctaattgc
     8101 tgtacgcaaa cgttcttcat ttaaagaaac agatggctgg ttaaataatg tatagcgtac
     8161 tattttgttt tcattttgac tttttactaa cttcatttct cgaatgtctc gagaaatcgt
     8221 tgcttgagtc acatcggcgc ctgcttcatg cagccgcgca agcagctctt cctgtgtccc
     8281 aataggttgt tctgtaatga tctggcggat cagtgcttgt cgctgtttct ttttcatatt
     8341 agacacctct caatgttgat tatagcaaaa gataagtaat atttcttcat attttggtat
     8401 tttttacttt tattttttgt tttcattcag gttgctaacc tttttctatt ttaacacgca
     8461 aaatcgtctt cattttttca ggtgctgatt ttcttggatc gctgatgtaa atctctttgt
     8521 gttctttaga agctctttca tagccttctt ttgcgatgaa tgcttctaat ttctcaaacg
     8581 ttgctggttc ttcggcatag ggcccgacgt gtaagatttg ggcacaaaga ccttcttcca
     8641 tctttttgaa tactacttgt tctttcaagt cttccggaag tttgctagaa cgctgaatgg
     8701 cttcttgtgc tatcgcttct gtaacaaagt caggctgtcg gatcatcaat tgataagaaa
     8761 aatgttcttt tttcatcact ggttcatgca aatacttttc ttgcagtccc cattgtcctt
     8821 ccagtggata gacagtatac atctgataat caggaatcag ccaatttttt ttaggcgaca
     8881 tgcgtattgc atagctgact gcatatagac aggctactct tcgtttaaag tcttcctgat
     8941 tcgggtcgcc gatccctctg atgctaaaga atgatttttt aggaacctct agtatttgcg
     9001 ggcttttttt catactatag ctcttgtctt tacgccattc aattttttcc atttttcttc
     9061 ctccgcgaaa ttccatttag ttatttatta tcatacctct ttttgagtga cttggctaat
     9121 cgtgttgaca ctatttttca gtttgtgcta gaataagtga agtaaataaa tcgtacagat
     9181 aacacgtttc ggcgtgtcga gagagaggcg gtttctattt ttataataga aatcactctc
     9241 tttttttacg aaatttttta gaggtgatgt aaatgacaaa acaaaaacag ctgcttttag
     9301 tacctgcagt aattgcaaca gggatgatgt cttttgcggg cgtattgatc gagacggcca
     9361 tgaatgtaac tttcccaact cttatcagac agtttgattt aacgactgca caagtccaat
     9421 gggtaacaac gatttatctt ttgatgattt cgatcattgt tccgctgtct acttatttga
     9481 atcgcaattt tagtctcaaa aaactctttt tagcttcaaa tctgcttttc ttggcaggta
     9541 taatgatcga tttcttctct cctaccttca tggtcctatt gtttggtcga ttgctccaag
     9601 gtgcagcgac aggtatcgct cttccgttga tgttccatat tatcttgaca tttgcccccc
     9661 tcaatcgacg aggcgcgatg atgggtgtcg gtactttgac aacggccatt gctccagcaa
     9721 ttggccccac ttatggaggc attatgactt cacattttac atggaatcat atcttcctgt
     9781 ttcttgtgcc cgttttgatt ctttctttga ttatcggctg gtttgctatt cccgagatgg
     9841 cagtacaaaa aagtggttat ttggattgga taagcgtttt aggaatttgt cttttgttta
     9901 gtggaagtct aacatttttg agtacgttgg gaacagtttc cagctggatt gctttaatca
     9961 tcggcggtat tggctggttc cttttttact atcgaagtaa aaaagtggag catccactga
    10021 tccgactgtc tattctttca aataaaacat tccgcttgtt tttgattggc ttcttagttt
    10081 atcagttttt attacttggt gtatcttttg tcttgccgaa tttccttcag atcgttcaag
    10141 gaagtagtcc ttttgtcgct ggattagcga tgcttcccgg cgcagcggtt ggtgctttgc
    10201 tttctccttt ttccgggaaa atgctggatc aatatggtcc gaagaagcca atttttgctg
    10261 ggctgattct ttctttgatt ggttggctag cattattcct actcatagga cacgcttcag
    10321 tgggtgtatt gattgcttgt catgtgtttt atatgatcgg tatcggatta tcttacagca
    10381 atatgatgac caccggaatg aatgcactga atgaagaact acaaggagac gggaacgcaa
    10441 tgttcaatac cttgcagcag ttttctggcg ctgtggctac ttcattagta gcggtcatta
    10501 tcaattacgt ccagcatcat acttcccata actatgaagt gtctactaca ttaggatcaa
    10561 aagcagcatt aggcgtcttg ttgctgttgc ttctcgtaag ctttgctcga ttcgcctatt
    10621 atctgttctt tgctaaaaaa gcgtagattc catatacggc aggtgttgca tctgccgttt
    10681 ttttgtgtga aaatggttgt ttgtaaaagg aggctttttt atggaaaaaa ttgccattat
    10741 cggtggcggg atcattggta tgaccctagc cagttactta gatacatcaa aattcaatgt
    10801 tgctttattt gatgaaggga caggacaggc aactaaagct agcgctggga ttatctcccc
    10861 ttggttgtcg aaacgcagaa acaaacaatg gtatcgtctg gctaaagacg gcgctgcttt
    10921 tttccctaaa cttataaaag atttccaact tgatcaaacg atttacgcac aatcagggac
    10981 acttttgctt cgacctgaaa aagatttagc tgaattggct gttcttgccg agaaacgaaa
    11041 agaagaagcg ccagaaattg gaaacatcga atggctttca cctgaacaga catctcaagc
    11101 acttccgtta ttgaaaccag cagtttcact gcgtatcagt ggtggtggtc gtctggatgg
    11161 aaaagcatat ttagaacatc ttggtcagcg tttagcacaa aaacaaatgg agcagatcca
    11221 ggcaaaagtc actttgcata agaagagcga ttcttggctc atcaagggac cgtttggaga
    11281 atacacagct gatcgtgtaa ttttgacacc tggcccggca cttaagcctt tattacaaga
    11341 attaggctat gctgcagata tacgtccgca aaaagggcag ctcttagcat ttcagacatc
    11401 ttataagatg agtcaagact ggcctgttgc tatgcttgac ggagaagctg atttcattcc
    11461 tttcatggat ggaaagatcc ttttaggtgc tacccatgaa aacgatcaag gctgggattt
    11521 ggctcctact agcgaagctt ataagggatt gactaagagt acagaacagt tcttgactga
    11581 tccagatcag ataaaaaact ggccatacga gttccgtgta ggtacccgtg cttatacgtc
    11641 agatttttcc ccatttttcg gcgtactgcc agatgatcca agcttactgg ctgctagcgg
    11701 tctaggctct tctggcttga ccacaggtcc ttatatcggc tatctattag ccaacttctt
    11761 aaatactggt cattggccta cggaagatta tcaaaaggac atctccacct atattacgaa
    11821 aaatccatca tagaaaaaca gctttttcaa gtaaggatcc cgagcgatat tgcgcatgga
    11881 ttacttcttg aaaaagctgt tattttagtt caatttctga atcaaattct ccaaactttc
    11941 ttttgcttca tctggcaaga gatcttttcc tatctgagct tcaatgaatc gtaaacgaat
    12001 atcaagtcgt tgtttcacca gatttttata ggacgtatcc tcaaaagaaa caggaaaatc
    12061 tggtataggt aaatagtgga tgtcttcaaa tggaccaaac gacttgaatt ctgccgtttg
    12121 ttgcacgatt ctttcaacga ttgacaatgt cgtttcaggt ttgatttttt tatcatcgaa
    12181 atatccagta ttcaggacat agcaggctgt ttgctgtttc aaaaacaact ctcgaaaagc
    12241 ttgatagtct tccctaagag gatagacacg gaaaggatta gcaaatggtt caatgactag
    12301 ctcatctagc tgtctttttg tagtgatatt ttccgcgtct gatcgttttg ttgctaatgt
    12361 tgcaccaaaa gacgctgcta aaactgggtc attgatcttg ataattggcg gaagcgaatc
    12421 atctttcatg atccaaaaaa cagcatcgat cttttcgcta agatgatcga ctcggtcggg
    12481 tgtcgcataa cgtgatttga ctgtccgccc atttccgttt cgtatatctc ctgttactag
    12541 gactttttct cctttacttg tacatgtcac gcccacattc tgacaagtaa ggaaagaaga
    12601 aatggccgga tcatcagacg gatagtcttg tgttttatca aagtaagaag gttccaaggc
    12661 agtagcaaaa ccattttttc gagaaataac gaaagcatcg tcatgcaaga tcgaaacaga
    12721 gtgaccttcc gtatgttttg ctaaggtaat cgttgatttt ccagaaccgg acaacccaaa
    12781 tacagccatc gtataggtct ggtcattact agcatattgt ttcatcccac cgtggcaggc
    12841 aataaagcca ttgcgatttc ccaatgccca agcaagcgtc aaagtcgctt tttttaattc
    12901 tccaaagtaa cgtaatccta gcacaaccgc tgtattatgg agagcatcaa acaaaatgag
    12961 tccatttgga aatgtttcat gtgtccattc gggatcacaa aagatataaa gatctccctc
    13021 atcaaaggaa acagactttt gataagtctc taagatcttc gaattcgctg gctgaaaatt
    13081 cagcaaataa gaatacaaat tgttctcaaa tccttctgga atcagcaaat gactttgcac
    13141 agaaaattct tcagataggc caacgattac ttttgtatgg taaaactggc gcttgcgact
    13201 ttgatacaca gcttctctta aaattttctc gtatttgctt tgttccttgt ctaagtcctc
    13261 aaagatccgc cgagcacttg ctgttcttcc cacaatgctt ccatcattga atactagtgt
    13321 cctaacatca gctggtaaat ctagttcttc agcatgtgag attgttaaat ctgtgatgat
    13381 cgttcctgga ctcccttttg ctagttgata agctgtcttc ctatcaaaaa cttcatgaac
    13441 attattaccg taaaatgtcg tttctacaat cgatcgaata ctggataaca gtggatttcc
    13501 acttgtgatt tcgtttaatt cgagactctc ttttgtactc atggctcctc ctcattccct
    13561 aaacaaaaaa acgttttctt tattatacat tattcgagtt tttatttgta ctgctgcgct
    13621 gtgggaagca aacgagttat ttttcacaca aaaaaaccga gaacttgaat aaacaagttt
    13681 ctcggtttca tcattttcct tttatcggct tagtaccagc cgtttgctaa ccagaatgtt
    13741 ttagcagcat cccatgaacc ataacgagat gttacatatt gttctgctac acgttcttga
    13801 ttggcagctg aatagtcgcc gtttaagtat gaagcatcta attgataacg gccgatgtaa
    13861 cgtccgttag tagctgtgta tgaaccgcct gattcttttt gtgcgatcca ttcttttgca
    13921 gagcttgaag ttggcgctgt tgcagcagct ggggcagcag gagctacttc aactgtttca
    13981 acaggtgcaa cgtatgtatg ttcttgcgtt gctgtttcag ttgtagatgc tgtattttct
    14041 gttgttttgt tttctgttgt tgctgaagaa tcatttgaag ttgggattgt taattgttca
    14101 ccaacataaa tgcggttgat gtttgcgatc gaattttttt cagcaattgc atcgatcatt
    14161 gagttgtctc ctgcaaattt ttgtgagatc ttagataatg aatcacctga ttttactgta
    14221 taaacttcat cagcgtgagc atttgtagtt cctacaaaca ttgcagtagc accagcagca
    14281 aaagttgtac ctaaaacaat tttcttaaat gaattcatat taaactcctt tttttctccg
    14341 ttttttattc tttcgacgct attaacttta tcatttagaa atatttcata ggtaaaagaa
    14401 acatgacatt taatgacaaa ataacgaatt atttaatatt tgtaacattt aaacaaatat
    14461 ttttttgata caaaaccata aaagccttgt tgtataagca aagtaatcgt ttttctatgt
    14521 ttttttattt ttgaatgaaa aacgcagatt ctgtaacatt cactgcaata atccataaaa
    14581 aaaagcaaaa aaatttttga tttttatcaa attctcttaa aaaaatgtaa aaaatacaaa
    14641 aaaaccagta attgttacaa caattactgg acaaaaactt tgaataatgt taactatttt
    14701 tttattttaa ctttctccgc aataatttct gggcatgttc caattcgttt ataacgactt
    14761 cttctgtttc tttagctaat atttcatcta atacatcaag ccatttttcc ggttcttttg
    14821 ttcctagacg tccaatagcc caagctgctg ttcctctgat gacagggcgg acatctgtta
    14881 aacattctga aatctcaggc aatgccgttc gatcacctag attagctaaa gcaatcaatg
    14941 cgtttctctg caaaggtttc ttcccacgcc atgaaccagc taaatgccca aattgctgtt
    15001 tgaagtcttt attagacatc gaaagcattg gctttaattt tggatatatt tcgtctactt
    15061 ttggttccat ctcttcatga aaatgaaaat cttttccttg attgtaaggg cagactaact
    15121 ggcagatatc acatccataa atgacatttc gcattttttt acgatattct tccggcatca
    15181 tcccttttgt ctgagtttga taagacaaac atcgctgggc attcattctt ccgtcaccaa
    15241 gcaatgcttt tgtcggacaa gcagtcacgc agcgcataca gtcaccgcaa ccaaattctc
    15301 ctggtttatc aggctcaaat acaatattag taacgatctc accaagatag acaaatgaac
    15361 cgaactcttc agtgatcaac aatccgtttc gtccaataaa tcctaaacca gctcgctgtg
    15421 ctacagcgac atcgatcagt tctccagtat ctacttgcgg agctagacgc cattcctctt
    15481 ctttttgcca tttttctgct cttgtttgga tgaattcgat caatcggttc aaacggtctc
    15541 tgagtatatc atggtaatcg atcccccaag aagcacgagc aaactgcccg cgtttttcat
    15601 ccctaggcac ttcttcatgg atcctagtag gatacgcaag tgcaatggaa atgatcgatt
    15661 tcggctgatc aaatgtcatt tctggataaa gacgctcatc gatatttgga tgttcaaagc
    15721 cagaagtatg gccagctgct ttttgttctt ccaacgattc ttttaatgaa tcaaaaggtt
    15781 ccgccgtggt gaacccgatt ttgtcaattc ctaatcgttt gctttcttgt atgatttctt
    15841 cttttaatgt catggtcttc ctcgcttgct gttaacggct gtataaccca taatatagtt
    15901 tttctttttt cagctggaca aactggatct gctgatcggc aatcttcttt tcttccatcc
    15961 atttttttct tgcagcaaat acttcccaat aatttgttga aattaatttt cttttgttat
    16021 ctaatgcatt aaaaaaattc aatgaaactg gttttgtact taacagttca tggagcctta
    16081 cctcttccca tggcgaaaac tcagacagat cgcgggaata caatacttct tcatagagat
    16141 ttttttgctg tgtgtacgtg aatgtttgcg ggattttccc aggcctagca atttctacta
    16201 gacgattata aaacatattc attaatttgc tgttggcaat aaattgactt ttataatagg
    16261 ccgactggat ctttttgatt actccttctg ctttgatgac tgaaaataat gcaggccatg
    16321 ctttgacggt tatttcgtcg ttttgtttaa ctaatacgcc aggtgtgaag ccgactggag
    16381 aaaggaacac aaaataaccg ataccggtat tttcatctaa aggctgaaat tcatcaaaca
    16441 accatttgcc tatgtacagc tcttcatctg gttctgttgt ctcattttga tttgctatga
    16501 ataatttagc taattctgct tcaagaaatt gaaggtcttg atccttgtcg gtattttcct
    16561 gctggctgat caatcgaaag acttcttctt taatcgtata tttatcttta cgtgtaaaat
    16621 tcatagccac accttccttc cttactctaa ctataacgga atttcttcaa aaaaggtatc
    16681 tgtacgacga aaaacagcat aatttttttt catatctctt ataaaaaatg caaggtctgc
    16741 tcctcaagct agaccttgca cttttcagct ttcttttgtt tgtgacaaac cttcatggat
    16801 cgtttcaata ttccatttca tcatctgata gtaagaatct ccttgttctc cttcttttgc
    16861 tatggaatca gtgaacagtt tatcatagat tttcaaaccc gtttcttttg ctacccgttc
    16921 catgcttctt gagtctacac tggtttccac gaataaaacc ggtacttctt ttgctcttat
    16981 ctgatcaatg atcgctttca tttgatcagg cgtaccttga ctttctgtat tgatctccca
    17041 tatataagct gctggcaaat catatgctcg tgaaaaatac ttgaatgccc cttcacttgt
    17101 tactaataat tttttgttct cttcgataga agcaaaactt tccttcgctt gtgtatctaa
    17161 ttcttttagt ttttccacat atgcttctgc atttttttta tagagtgtcg cattttctgc
    17221 atctttttca gagaatatac gtgcgatttc ctccacatat tttattccgt tagataggtc
    17281 tagccaagca tggggatctg cttttgtatg ttcttcaccg ctagttaaat aaagaggttc
    17341 tacatttttg ctaactgcaa aataatcttt tccttcttct tttttagccg tttccatcaa
    17401 gttatcgaac cagctgttac ctgtttcaag attcaaaccg ttgtataaaa taacatctgc
    17461 atcgcttgcc tttttgatgt cttctggtag tacttcatat tcatgcggat ctgttccgac
    17521 tggtacgata ctgtggatat ctatttgatc tgttccgacc tcttttgcca tgtccgccaa
    17581 gatcgaattg gtagccacga ctgacaactt ttctttactt ccttcatccg cttggtcaga
    17641 agcctgcttt ccgcaaccgg ctaataccaa gaaactaact gccaagatta aaaagagtga
    17701 tttttttgtt ttcatctatc tgcttccttt ctgaaataaa attccttttg ttggagaaaa
    17761 taaaaaagca aggataaaaa atgcggtaca tgctaacacg atcgtagctc ctgatggcca
    17821 attgtaacta taactgaaaa acacaccgac aactgcactt agcataccga ttcctgcagc
    17881 tgtaatgatc attttcagta aatggttcgt tagcaagtaa gccgtggcgg ctggtgtgat
    17941 caacatggca atcaccaaga ttgttcctac tgtctgtaaa ctgactacag ccactaatgt
    18001 caaaaagaac atcaaaagat aatggatcag cgatgtgttc agcccataag cttttgccat
    18061 tgtcggatcg aatgatgtga tcttcaattc tttataaaag aaaaagacaa atatcaagac
    18121 gattccacta acgagtgctg tcagcaacaa atcgctttcc ctaacagcta agacattccc
    18181 aaataaaata tgatacaaat ctgtcgcact ctgggcatat gaaatcaaga taatccctaa
    18241 tgcaaaaaag gagctaaaga caattccaat cgctgtatca tttttcaatc ggctccgctg
    18301 agtcacgaaa ccaatcactg ctgctgttag catcccaaaa gcagtggctc caaagatgta
    18361 actaaaacca aacatataag aaatggctac acctggaaga actgcgtgag agatagcgtc
    18421 tcccatcaaa gacatccctc gtaaaataat aaaagaaccg atgactccag aagcaagtcc
    18481 tacaagcatg gaagtgacca aagcgtattg caggaattga taattcatta atccatcaat
    18541 aaaggattgg atcatttttt ctctcctcct tttactaaat gtctgatgat ctcaccatag
    18601 gccaattgaa ggttcttcga tgtaaagact tcttgtactg gaccggaagc gatcagctgt
    18661 ttatttaaaa gaataacctc atcgaaatat tcttctactt tatgaagatc atgatggacg
    18721 atcaaaatcg tttttcctga attcttcaat tcctgcaaga tgtcaaagat ctttcgttca
    18781 cttaacgcat caatccctac gaatggttca tctaaaaaga tccattctgc tccttgggct
    18841 agagctcttg caataaaaac tctctgaagc tgtccacccg atagttcgct gatctgtctt
    18901 ttggcatatt cttccatccc tacttttttc aaagcttgtc ttgcacgttc tttttctttt
    18961 ttcccaggtc tttgtccaat tcgtaaagat ggatatgttc ctaaaagtac aacgcctaat
    19021 acatcaattg gaaaggaaag atccaattca cttcgctgtt ccacgtaggc tattttttta
    19081 cggactgagt ttaccgactg tccagaaaag gtgactttac cagtagtagc tggtatcaac
    19141 tctagcaaag atttcataaa tgttgatttt cctgcaccgt ttggtccgat aataccggta
    19201 attttttgtg gatttatcgt caagttgata gttttcaaaa cagttttccc ttgataagcg
    19261 gctgaaaggt cttgaattac aatggccgta cgttccaaat cttacactcc tttttaggtt
    19321 tacctaactt tttatttaaa tatagtttaa ctaaaatgga aagcattttc aagtagaatg
    19381 atgaaaaagt ttttttcgaa ttttcctcgc attttatgaa aattttctgt agaatcacat
    19441 caagagttca ataaggctaa cctaataatg aaaaacatgt gttgaaacgt ttgattattt
    19501 tttcttgttg actcaacaac gaaaggagtt tttgtatgga agaagaaaag ttaactcgaa
    19561 gtcttagtgc ccgtcatatc caaatgatcg cactaggtgg aacgatcggt gtcggtctgt
    19621 ttatgggtgc ctcctcaacc atacgttgga cagggccctc agtgatggga gcctatgcga
    19681 ttgcaggatt ctttttgtat ttgattatgc gtgctttagg cgaaatgctt tacgttgatc
    19741 cttcgactgg ttcttttgca aattacgcga gcgagtacat ccatcctgtg gctggctact
    19801 tgaccgcttg gagtaatatt tttcaatata ttgtcgtagg aataagtgaa gtgattgctg
    19861 taggatctta tatgaattat tggtggcctg atttgccggc aattattccc ggaatcattg
    19921 tcgtcttgtt tcttatgctg gcaaatctta tttctgtcaa ggcgtttggc gaattagaat
    19981 tttggttttc gatcatcaaa gtcataacga tcattttgat gatcattgca ggtcttgggg
    20041 taatcttatt tggatttggt aatcatggtc aggcagtagg gatttcaaat ctttggaaaa
    20101 atggtggatt cttcactgga ggcgtcaaag gatttttctt tgcattatcg atcgttgttg
    20161 cttcctatca aggaatcgaa ctgatcggaa tgactgctgg tgaagcagag aatcctaaaa
    20221 agacgatcat tgaagctgtc cagtccacta tcggtcgaat cttgattttt tatattggtg
    20281 ctatttttat cattgtgagt atctatccgt ggaatcaatt gagtgagatt ggttcacctt
    20341 ttgtacaaac tttttctaaa gtcggcatta catttgctgc aggactgatt aattttgtcg
    20401 tgattacagc ggcattatca ggttgcaatt cagggatttt cagcgcaagt cgtatggtct
    20461 atacacttgc cataaatgga aagatgtcta aaaaatttct gaaactatct cgtcacggtg
    20521 cgccttttta tcctgtcgta gcaatctcag gtggaatctt aattggtctg atcttaaatt
    20581 atcttctacc aatgttcatc aaccagtcag aaaatatttt cgttttcgta tacagctcaa
    20641 gtattttgcc tggtatggtt ccttgggtag tgattttgat cagcgagatt cgcttcaaaa
    20701 agaagcacgc agataaaatg catgatcatc cttttaaaat gccactatct ccttatacga
    20761 attatgtgac acttgctttt ttagctatcg tattgatttt catgtttatc aatcctgaaa
    20821 cgagaatctc attgattatt ggactcgttt tcttgcttta catgacgatc cattactttg
    20881 tacgcgaaaa aagaaccgga aaataaaaaa agaacgaagc ttcaccttag tggaagcttc
    20941 gttctttttt attgctttta tcaattcaat tttagcgtct caccgggagt caggaaataa
    21001 ttctccatgc tcatcccatt cagcgacaag atcgtttcta ctggtacgcc agttcgttcg
    21061 gctatttgat ttggaccttc tccttccaaa actacggttg tcgcagcctg tgaagtcgcg
    21121 ctgttggtat tagtttcaga ggaatctgtt gtgtcttgtg ttgttcgagc ttgcatttca
    21181 gaagcttcta ccgactctgc tgtctcagaa tcagaagatg ttgtgtcttg tgtgcttgtt
    21241 gactctgctt gaagagaggc gctagtctgc aaataaatat cggaaatctt agcgacatga
    21301 ttcgtataat tagccgataa ttgattgatc aaagtatcta agtctacacc gtctttattt
    21361 tctgctgcct gatacgctgc acgtaatttt ctgatccctt cgtcggagat tgcttgaagc
    21421 tcacgtgctt tttggttagc aactgctgat aaaccagcta ttccctcttg atttccctga
    21481 atttctgtct ggtattcttc aatcaatgca ggcgtctttt cttcgatctt tttcgaataa
    21541 tcatttacga ttgcttgaag ttgctctggc aaaccatttg ctgttttttc ttcagaagtt
    21601 tgtgtctctt ttgttttagt tgatgactca cttgtttgtt tcgtctctgt tttctttgtc
    21661 gttgtttccg ttatggtcgt acttttcgct tctgacgaat tttttccatg aaaatataga
    21721 cccgcaactc ctcctgctat gatcaaaaac acggcaactg ctgccccaat catgatcatc
    21781 cgcttacgag ctgcttgttt ttgctgtctt cttcgggtca tccgaacatt ttctccccac
    21841 tcattttcat catacatttt tttcaccacc aatttttatg tataatagcg ttatcaaacg
    21901 cttcatattc ttattgtagc aaataattgt cgctgtgcac atttttatct ttgttattta
    21961 taagaaaatt tcaccagttg agatgattcc aagattgtcc gtcgatttct ccttgcagct
    22021 gcattgttcg ttcgtctatc tcagccatca gtttctttat ttcatataag agcgcacggt
    22081 cttgatattc tgatttttcg cttaattcat ctaattgttg gatcagccaa tcatttgtca
    22141 taatgaagtt cctttcattt cttgcagtgt aaaggtcaag tcttcgatct gctcttttgc
    22201 ctcagtcagt gcttctacga tatatgcttg tttttcagat tcttcttcct gcattttctg
    22261 attttcctgt actctttgaa taaaccagtc aatccgctga gtcagcgatg gtttgccttc
    22321 caatgagaga tactgctgat aagcaattag cttctcccaa cgtttttcct gggataaata
    22381 cataaattgt gctaaaacga atggcggaag ataggtcata tggaatttat ttgcgattcg
    22441 ctgatacggc ctcaaaaatt cggaaatcgt atattcttct tttcctccag cttggtattc
    22501 tttttccgcc acacctgttg tcaatacgat tcctagttct ttccctttta atacatgtgc
    22561 tgctcgttcc aagacattat cttgccaaat ttttaaatga gcaggagaac tataccagta
    22621 aaacggaaac tggaaaatga tccgatcatg ctgggccaag agctgtagtt cagtctctat
    22681 atctattttt ccgtctggat aacttgattc caaatgatgc caagtagctg gtaaaccagc
    22741 agaagcttct ttgaaaaatt gctgatttaa agattcttcg atcgttggat ggctaatgat
    22801 gataagtgta cgcatccctt ttcctccttt ttagtcttcg ctttacagtg tagcataacc
    22861 gccacaagtt tcttttgccg atgcttgtca ttttaaccag actaatcagg ttgtccaaga
    22921 aactgcggtt tgtttttcca actgtttttt caaggtatcc aaagcaatat tcttaaaatt
    22981 ttcgtttttc atacgtttac gatagttcgt agccgtttct ccggtactct ttttgaacat
    23041 tcggctaaat acgactagac tattgaaccc tacttgatag gcaatacttt ccacggattc
    23101 atccgtcaga gaaagaagaa acgcccctcg attgatccgt accccgatca aatattcctt
    23161 gatactggta cccatatgat ttttgaatac tcgcgacaaa tggctccgat tgaccgctaa
    23221 atgctgggcg atttcctcaa cagacaattt ttcagcataa tgtgcctcga tgtatttgac
    23281 tgtttcaaca gctaaagagg aatgaacctt ttgtacacca ccgctgacct ttccgccatc
    23341 ttttaaaagg actgataaaa aacggtaggt taatgctgtt aactgtaatt cattttccaa
    23401 ggtttcttcc gtatctttca tgcattctaa aataatgtcg atatattttt gtatgtcaga
    23461 agagaccatc gtgtattggt cttctctgaa aagcgactgc cgaatgattt cttctgcagc
    23521 actcccacca aatgaaagcc agcaatacat ccacggatct tctccatctg ataagtaaaa
    23581 cgtcgagtct cctggacgaa tcaagaacag atcacctttt ttcaattgga attgttgatt
    23641 tttcacatga taagatcctt tgccttccat cacgacatgc aggatgtaat cattgcgaat
    23701 agcaggacca aaagaatgaa acggcatcgt tttagagaaa ccacaaaaat caaaatacaa
    23761 ttctttttct acttgttgat gttccttttt taaaaaaatc gattgctcca cgtctatccc
    23821 tccttactcc tttagtataa tgcaacaaaa agttaaataa aagtcacaga aatatattaa
    23881 aaaggaagcg atttcttgtt aagtttaagt cattaaaaga tctttgttat tcgttgtaac
    23941 cggtttactt agtattatat attgaacagg agaggaatac agattatgaa aagcaaaatg
    24001 atccaacgtg tgtcctacgc tttaggcgct tttggacacg acacattcta tgttacatta
    24061 agcacttatt ttatggtatt cgtgacaagc cagatgttcg caggaacaga taaggcaacg
    24121 aatgcaaaaa tgattgctac agttacaagc ttagttgtag gtatccgctt agttgaaatt
    24181 atttttgatc ctttgattgg tggcatcatc gataatactc gtacaaaata cggtaaattc
    24241 aaaccatggt tagttatcgg cggcgttgtt agttccgtac tcttggtagc aatctttacc
    24301 aatttctttg gcttagctac tcataatacg actttattta tcatattatt tgcaatcgtt
    24361 ttcatcttat tggatagttt ttattcattt aaggatatcg ctttttggtc aatgattcca
    24421 gctttgagta cggaaacaaa ggaacgtgaa aaatttgcta cgtttgctcg tttcggctct
    24481 tctctaggtg caaatggtac tactctagtc gttgtcccta tcgttacttt ctttacctat
    24541 cttgttactc ataaacatat tgaaggcgct tcaggctggt tctggttcgg tgtgattgct
    24601 gctgttattt caggcggtac tgcgattatc actgccttgg gcacaaaaga agtcgatact
    24661 gcgatccgtc agcaaaatga aaaagctggg atcaaagatg tgttcaaagc agttgctcaa
    24721 aatgatcagt tgatgtggct tgcccttgct tatatcgctt atgcgattgc caatgtagca
    24781 acaactgccg tattattcta cttcttcaaa tatgtacttg gccaaccaac tgaattttgg
    24841 atcgttggtc tagttgctgc cttacttggt ttagtagctg ttccactatt cccaatgctt
    24901 acacgcgtca tctctcgtcg ctacgtttac cttggaggga tcgcaatgat gatttcttct
    24961 tactttttct ttacgatcgc aggttcaagt ttgccaatcg ttatcatcgg gctagtattg
    25021 ttctacttcc ctcaacaatt gattttctta tccgctttga tgacgatcac agactctgtt
    25081 gaatatggtc aatggaaaaa tggtatccgt aacgaagctg ttactctatc aattcgccct
    25141 ttactagata aaatcgctgg tgctttgtct aacgggatcg tagggtttgt cgctatcgct
    25201 tctgggatga ctggtaatgc tactgctgct gatatcactg ctcatggcgt aacgactttc
    25261 aaagcttatg cattctatgc tcctgctgct ttgatgattt tctccgctat tatttttgct
    25321 tggaagatta cattgacaga gaaaaaacat gcacaaatcg ttgagaaatt ggaaaacaaa
    25381 ttggctccag ctgatacaat ggaagatgaa cttcacgatg cagtagttcc ttcccattaa
    25441 gaaaggatct tgaaaatgaa gattacagaa aaagattttg gccaaggtta tcacttgata
    25501 accttggaga acaaaaacaa gctagcactt tctatctctg atttaggtgc tagaatcgtc
    25561 agtttaaaat caaatgatcg agaattagtt ttgggatttg acaccgctga agaatatatc
    25621 gaaaaagatc cgtatatcgg tgcttctatc ggtcgcaccg cagggagaat cgaaaatggc
    25681 cacttttctc tcaatggaaa aacttaccaa ttagccacag atccaaaaac tggacataat
    25741 ctgcatggtg gtgctcctgg atttgaatta aaaaaatggt cttatgtcat tttgaatggc
    25801 gaaaatgaag cttctgtcat ttttactact acaagtcccg acggagaaca tggttttcca
    25861 ggtacaatgg atgtggaaat ccgctatact ttgacaaaag ataatatctg gcgagtgacc
    25921 tctcgcggca ccagtgacca agatacttta ttcaatccga caaatcatgt ttacttcaac
    25981 ttgacgggag atgcttctca gtcaatcgat caacatgagc tttggctcaa tagcgaagct
    26041 tatgctcctt tgcgaacaga tagtattcct ataggggtaa aagaaaatgc tgcaggaagt
    26101 gctttcgatt ttcaaatacc taaaaaatta gcgtccgttt ttgctagtga tttggatcaa
    26161 aaaaatcttg ttgacggaat cgatcatcca tttttcttaa aagaaaccgg cttgggtaaa
    26221 gaagccgctc gcttgactag ccccgataaa agaatccaag tagatattgc tacagatgct
    26281 tctagtgtgg ttattttcac tgcaaatttc ggcacagaaa caccagaaat gcgcaaccga
    26341 aaactggcac atcacggtgg catcacattt gagactcaaa cagctccagg tgcagagcgc
    26401 ttctcagcat ttggttcgat tcatttaaaa gccggatcag tatttgaaac agtgacagaa
    26461 ttcaaaatca aaacaagaaa ggaataactc atcatggaaa tcaaaagaag cttaaacaca
    26521 aaatttgccg aaacattcgg acctaaaaaa actacacaat acttctcacc aggacgcatc
    26581 aatttgattg gtgaacacac agattacaat ggaggccatg tcttccccgc ttccatcact
    26641 tatggaacgc aaggtgtagc agcacctcga gacgataata aaatattagt ttattccaca
    26701 aactttgacg atatcggtgt cattagtttt actttagatg agctagaata tgataaaaaa
    26761 gcaaactggg caaattacgt aaaaggaatg gtccttaaat taaaagaagc tggacactca
    26821 atcgatcatg ggtttgagtt attagtggaa ggaacaatcc ctaatggtgc aggactttcc
    26881 agtagcgctt cattagaatt attagtaggt gttgtattag aagatctgtt tgatttaaaa
    26941 acagatcgct tggaactagt ccaaacaggt aaaaaagtag aaaatgaatt catcggtgtc
    27001 aattcaggta tcatggatca gtttgccatc ggcttcggtg agctagatca tgcaatctta
    27061 ttagatacaa atacgctgaa atacgaaatg gttccagtga aactagatgg ttatgcaatc
    27121 gtcatcatga atacgaataa gcggcgggaa ttggcagatt caaaatacaa tgaacgccgt
    27181 agcgaatgcg aagaagcctt ggcacgtttg caaaaaactt tgcctatctc ttctttagga
    27241 gacttagatg aagaagaatt tgaatcaact attgaccaaa tcggcgatga tacattgata
    27301 cgcagagctc gccatgccgt ttctgaaaat cagcggactt tgaaggcaaa agctgaatta
    27361 gaagctggga acttagaagc atttggtcaa ctgttaaatg attctcacca ctctcttcgc
    27421 tatgattatg aagtaactgg gatcgaatta gatacactag tagatgctgc tcaaaaacaa
    27481 gaaggtgtct taggtgcacg aatgacagga gctggatttg gtggctgtgc gattgctctt
    27541 gtcaaagaag aaaatatacc tgccttcaaa aacaatgtct atgatgaata catgaaagtt
    27601 gtcggctatg ctccggaatt ttatgttgca cacattggaa atgggacaac aaagatcgat
    27661 ccagaagctg ttcatgtttg ataaaaaaat caacgatctc agctaaaaac aaatagataa
    27721 aaataacctc tcagcaattt ccaaatgtga gccttttgct cgtatttgaa aattgctgtt
    27781 ttttattgac acggaaagag aacgtgtgtt ctaattgtgt tgaagaggtg attgttatgt
    27841 gggcgaatca aaatccggta tttgtttatg aaaaagaacc ttcaagagat attttatgta
    27901 ttgactgcaa atctttttac gcctccgtag agtgcgttga aaaggatctt gatcccctta
    27961 caactaaatt agtagtcatg tcctatccgt ccgattcgac agaaacacgg ggtagcggat
    28021 taatcttggc ttccagtcct gcagcaaaaa aagcttacgg catcaccaat atcagccgtg
    28081 ccagagatct tcccttcccc tatccaagtg atttatatat tgttgcccca aggatggcct
    28141 attatatgga aaagaacaga gaaatcaatt ctatctataa aaaatatgca gacgaagcga
    28201 accatcatgt ctattccgtc gatgaaagct tccttgatgt aacaaatagc atggcactct
    28261 ttggtaaaac aaatgcgtac gacatggcta aactgatcca taaagatgtg tttgagcaga
    28321 ccggaatcta cacgactata ggaattggcg ataacccgct acttgctaaa ctagccttag
    28381 acaatggctc aaaagacgga aagcaaatgt ttgctgaatg gcgttatgaa gatgtaccag
    28441 aaaagttatg gaaaatctca gatatcactg acttttgggg aatcggcaaa cgaacagcaa
    28501 aacgtctgaa acaaatgggt atacattctg tttatgattt agctcacgcg gattattatc
    28561 acttgaaaag caagctaggg gtcatcggta ctcagcttta tgcccatgct tggggcatcg
    28621 atcgaagttt tttgggacaa gaatatacac caaaatcaaa aagtatcgga aatagtcaaa
    28681 tcctagatag agattataca agacgagaag aaatcgagat cgttatcaaa gaaatggccg
    28741 accaagtcgg cactcgcttg agacgatctg cagcaaaaac acaagtagtc agcttatggg
    28801 taggatactc tttaggctat cttgatccaa atggcaagac cgggtttcac cagcaaatga
    28861 aaatcgaagc gacaaatgct agcagagatc tagcagatgc tttattgacc attttccacc
    28921 agcattacca ctaccaggat attcggcgtg taggcgtgaa ctgttccgac ctcgtatacg
    28981 cgacaggttt acagcttaac ctttttgaag atcctgaaaa gcagataaat gaagcgaaag
    29041 ttgattttgt tgtcgatacg attagaaaaa aatacggctt tcaatcaatc atccatgcgc
    29101 atagtctctt gcaaggagga cgcgccatcg cacgtagttc tctcgttggc gggcatgcag
    29161 gtggtatgag tggattagaa ggtggaaaag atgagacgga cagcaaaaaa attcattgac
    29221 tataacgagt atcgagatcg gccatttgga ttaaaatggg ggactgcttt tgcgatggaa
    29281 gaactagtca acggtatcaa aaaaaatgaa gaagaagcac gcaaacagcc tcttcctaaa
    29341 caacaaatga cattgcaaga aatcgatagt atactgacag aagccttttt atctcgtaaa
    29401 gcagtaatca ttcagctcaa tttaaaagat gaatttggaa gactcttgga tgaggtcgaa
    29461 gggcacttca ttggtgaagc ctatgaagat tactttatga ttgaccatca gccgatattg
    29521 tgggaagacg tacggtatat tgagttaaag gaaacaaaaa aatggtttgt gacatgagag
    29581 gttgtgacag aagtgatcaa cttcaaaaaa taagaaagga tttttcgaaa attgcttctt
    29641 aaatttttgt ggaattttgg cttatttttc gaagaagcaa cttctggaac aacgtatatt
    29701 agatatcaga ggttgtgaaa aagtcgctta gttttgagta acaagccaag gcgctgattt
    29761 cacaacctct gtctatctat ccatctattc ggtaagattc atgcatgacg tcttctttat
    29821 ttagtatgta gagccctttt tcttctgacc attcaacata aatcagctgt tttacatctg
    29881 catagccttc tttcttcaag ttctccataa gccatgcttc agtcaattga ttggactgaa
    29941 gcactttatg ctgaatttgc ccttcatcta caagcaggat cgataaagct ttgtcttcat
    30001 ctgatttttt caatacactc atttgaccgg cattctctag gataacatgt ttggcatttt
    30061 caagaaaaaa gcattcttgc tgtcttaaga ttgctcgcaa ctgctccatc tcgatatggt
    30121 tatttgtcaa ctctgtcatg ttgatgacgc cgtctttgat tagaacagat ggctcgcctt
    30181 tgacccaacg attgaccttt tcattttttt ggacgatacg ttcgatacca taaatcatta
    30241 ctgcccaaag cgcaatggca aataggacat gccctacatg gacattgtca tcataggttg
    30301 attcttctaa gatcccacct aaaacaagcg tatagactaa atcaaacggt gtgatttctg
    30361 acatggattt cttcccaagc aaacgtacca ctaataataa accgatcaac ccgaacacca
    30421 gtttcattgt aattgaaaaa atcatttttt ctccccctaa ctatctgtta attaaattat
    30481 atatgctttc tcacgaacac gcatggaata tacaaggaag aaatgtatcc ttttactaaa
    30541 aaaaggactg tgacactgta gtgaccccca aaagttagac ttttttggag tggagaaatc
    30601 cactcctttt ttttatgctc taaaatgata tttttacaca accgttacgg ttgttttacg
    30661 aaattggacg agactttgcc agtttaattt ctgttttatc cgttcattat tgtagtaata
    30721 gatatagcta tcgatggctg attttaattc ctcgaaacag ttataaactt tcccgtgaaa
    30781 aatctcttgt tttaataagc caaaaaaatt ctccataagt gaattatcta agcagttccc
    30841 tttccgagac atgctttgaa agattttgta ctccttcaat ttgtttctat aagctttcat
    30901 ttgatacgcc caaccttgat ctgagtgaaa cgtacgacga tagggacaat cgttagtggt
    30961 ctggatagct tcctccaaac cttccattac cgctaatgca ttgggctttt cagagatacg
    31021 ataggataaa atctcactat tgaacatatc cataaaagga tctagataca atttcttttg
    31081 acggataatg cctttggcat ccacttcatg gtatttaaat tcagttgtat ccgtcgtgat
    31141 tttctgatgg cagatactgg tgtagaaacg acgatggatc cgatttttgg ctactgctcc
    31201 aacttttcct cgataagaat tgtagcgacg tgatttacgc gtgtatgccg ttacttttat
    31261 tcctagcttc ttgatcaagc gctgaacttt ctttttattg atagagaaac cgcggtttct
    31321 tagttcattg gtcatccgta aacagccata atccttgtgc ttctcccgaa ttttaagcat
    31381 ttccgcttca atctcttgat ctggattttt acggtccaat cgcctttgcc atccaaataa
    31441 tcatgaacga tctttaattt aagttcaaaa gtatatttaa ccataaaaaa taccgacctc
    31501 caaaagttag attttttggt ctaacttttg ggggtcggta cacaaatcgt gtcacagtcc
    31561 cttttttgaa aataaacggt gttcaaaagc ttgcatctgc ggtaataagt ccagccaagt
    31621 taaaaacata agaagctgtt tttacttggc tgttcttatt acttgatgca aggcagcttt
    31681 ttcacaacct cttttcaaat atttcgtttt cttattctac aaatggttct tctcctaacg
    31741 cttgttgagc ggctagattc aacatgctcc attgtcggtc aaagcctggc tggaagaaga
    31801 agtcagcttc tgctagttct tctaaagtca gtttgttttg gatcgctagt gccaatacat
    31861 ttccttgacc tgtaacatca taggtagata atactgctcc tcctagcaat tgatgggtca
    31921 ttggatccga gaataattgg atggacactt ttggatttcc ttgttcttct ggtacatatt
    31981 ttggtcgtaa cgtatcttca taataagaca ctcgtacatc tactcctgct tttgctgctg
    32041 taaagctatt caatccgctt gctgcaaaat gatagtcaaa gacacttaat gcagaggaac
    32101 ctacgactcc gccgaacggt ttgtttggta cttcttcaaa aagatgtttt actacataac
    32161 gtgcttctct acgtgcaaca gtcgctaaag cgatcggcat ttttttgcgt gctgggatag
    32221 aataagcaag tgtagcatct cctactgcat acacatctgg aagattcgtt tgcaagtatt
    32281 catttgtttg gatccagcca cgttcatcta agtcgacgat cccttttaac cattctgtat
    32341 ttgccttcac accggcagct tgaatgacta gatctgctgg gatttctttt tcacttgttt
    32401 tgattgcctc aacttttccg ttaccagtaa aagcttcgat ttttgcattt gtcaaaactt
    32461 caataccgtt ttcatttagg tgttgttcta aaatatctgt catctcagca tctaaataag
    32521 tgcctaaagg tctatcgatc acatcaagca atacgacttc tttcccagct tttcggcttg
    32581 cttctgctgc ttcgatccca atgtaacccg cacctacgac agcgattttt ttaatagccg
    32641 gatcatttaa tttttctttg attttggttg cccaattgta tccacgcatc aagtagacat
    32701 tttctagatc tgctcctggt actggcagat tgtttggttt gacaccagaa ctcaaaatca
    32761 atttgtcata aggatattct tttgtttcgt tatctgatgc gctagtaacc gttactgttt
    32821 ttttatcggc atcgatcgtt tttacttcgt gattatttaa aatatgaaca ttggattgcg
    32881 gaaagttttc tggtctgaaa ttacgtacat catttacatc tgttaccttg tcttctaaat
    32941 acagctccat accacatgac atgaaggaaa tatagtctcc tgcttcaaat agcgtgatat
    33001 ctacattttc accgtatcgt gtcaatagtt ccaagatcga ttgatgccct ccatgagatg
    33061 cacctacaat gacaactttg attttttcca ttgatgaaac ctccttgaat attcttttaa
    33121 aatcattcta aacaatttaa acttaacatt acattagtta tgaatcaaaa gaaacgcgtt
    33181 catttgaact aaatttaaaa aacaatcgtc tccttgcaga atataccgca aaattaacga
    33241 ttgttttttt gataaaatct atgaatcact gttctgttaa aaagtctttg atccgtttgc
    33301 aggcctcatg taatttttcc atgctgctag cataagaaat ccggatataa ccttctcctt
    33361 ctgggccaaa tgcaattcct gggatacacg caacttttgc ctgttccgct aattctacac
    33421 aaaactgcca agagtcagtt tgaatggcag ctgggagttt gcaaaacaag taaaatgcac
    33481 cgtttggctg ggtcacttca aatcctaaag gcgccaattg ctggaccaga tagtctcttc
    33541 gtgtttgata ttgctctttc atattctcgc tatctttcac cccatttatt agcgcttcag
    33601 ccgcagcttt ttgcgacatc gttgtagcgg cagtcaccag atattgatgg actttaatta
    33661 gttcgtcaat aagggctttt tgagcaaaaa taaagcccac tcgccaacca gtcatcgcat
    33721 gtgacttgga taacccgctt atcacgattg tttgttctgg aagataagta gctatggaca
    33781 tatgcgttcc actataagtc aattcagcat atacttcatc acttataaca aataccggat
    33841 atttttttaa aacctcagcg attgctttca attgttttgc agacaatgtc gtcccagtag
    33901 gattgttagg atagtttaaa atgactgctt tgacagctga tccatattgt tcaaatgctc
    33961 gttcaaattg ttccggctga cagacaaacc ctgtgtcaga agtatctatc gggatcaatt
    34021 cagcaccagc tagttccact aatggctgat atcctgaata agcaggcgct ggaataagaa
    34081 ccttgtctcc ttcttccaaa acagataaca acgcagaggc aatcgcttcc gttgcaccaa
    34141 ctgtcgtcaa aacttccgtt tgcggatcat aagttaatcc ataacgctgt tgctgaaata
    34201 tacaagccgc ttcccgcagt tcctctaatc ctctcattcc ggtatagtga gaaaagtctt
    34261 cttcaatcgc tgctattcct gcttgcttga catgttcagg agttggaaaa tccggttctc
    34321 ccagggtgag tttgatcaca tctggtatcg acgatatttg ctgatcgaat tgtcgtattt
    34381 ttgatacttc tattctagaa agcctaggac taaatcgatt ggataaattc atcattattt
    34441 actcgctcct cttttgaact gctatcgatt tttacaagaa gattttctgt aaaaagaatc
    34501 gttttgtacc attctacacc attttctaaa acatccatat caaaatcgaa cgtcggatca
    34561 tgtaaagcag gggtatcacc gacacctaaa aagaaaaata cacatggata ttgttgcgta
    34621 tatatgccaa aatcttcggc ttgaagaact ggagcatcta attcatgtag tttgtttgaa
    34681 tctaacagtg ctgcatataa gcctctatgg ttgattacag ctggatagcc gtcattatag
    34741 cgaaaagaaa tagtagctgt agagtgaaac tgttctgcta aatggtctaa tcccctcttc
    34801 aaataatttt gcatatctga actgtaagaa cggatacttc cggatacaat aacttctcca
    34861 gctaaaacat ttcggatcgt tccaccttgg atttggccaa atttcattag atgattccac
    34921 tctgccggta attgccggtc aaaatcataa acattcttca aaaatcgtac agctgcctcc
    34981 agactatcga ttcctttttc actagaagca atatgtgcag actggccttt aatgataatg
    35041 tctgtttcac tgctttgtgc catcaaaggt cctggtcgag agaaaacttc tcccttagct
    35101 agtccaggcc atagatgaaa gccaaatatt gcttgtactt catatttatc caacaatcct
    35161 gactgaataa cgctttgtgc gccaccggaa atttcttctg atggctgata gatcagtact
    35221 acattataaa taagatcctc aatgtgttta gttataaagt cagtcaaccc taaaagcatc
    35281 gccatatgtc cgtcatgacc acatgcatgc atataacctt catgcttaga agcaaaagag
    35341 agattcgttt cttctttgac tggcagtgca tcgatatctg ttcgaaaagc aatcgtttct
    35401 tcttgctgat tatcaaaata aaggagtagt cctgtttcgc cgatttcatg gataacggcc
    35461 tcgctgtttt ttacttgttc atataaatat ttttttgtct tgaattcatg aaacccgatt
    35521 tcaggaattt gatggagtgc cattcgatat tctttcagca tccgtattca ccatcccttc
    35581 atagataccc ttcactactg tttcatattg attattcggt acaccgatga tcagattcaa
    35641 ctcctgtgct ccttgtgata agatcgatgt gcgaatttct aatttgttca atatcgataa
    35701 cactttccca gataatccaa tcaattcttt atgcggaccc ccaacgacag agatcaaagc
    35761 taagtcttcg atcacgacga tttcttccac gccaagtgtt tcttttaatt ctttcgtgat
    35821 gagaaataac ttatcggcaa tcgcttctgc tgagacgatc actccgatat tgtcgatccc
    35881 tgaaggaata tgttcgattg atatctcatg tttagcaaaa atgctcattg ctttccagat
    35941 aaacccgatt tcatcggaca tatgacgttt gaataaagtg atggaaagaa aatccttgcg
    36001 tccagctatt cctgtcagtc cattttcctt ttctatttcg tgattcgata tttttgtccc
    36061 ttgagcgaaa ggatcattgg tattttttat ttggatgggg atatcagcct cttgaactgg
    36121 gaaaacagct gcttcatgaa gaacgttagc ccccatataa gccatctctc tcagttctct
    36181 aaaagagatt tcctcgatga ttttaggatg atcgatgatt cgcggatcag ccatcatgat
    36241 tcctgaaaca tccgtccaat tctcataact ttccgcttgc aacatcttcg ccagtatcgc
    36301 gccggtaatg tcgcttcccc cacggcccaa taatttttct tttcctgcag ggttcgcccc
    36361 gtaaaaccct ggaacaacca ttttcggata tcttttaaat gcttttgtga tttcctgtgc
    36421 tgttttttcc tgatcgattt tcccatcata gtcaaagaaa atcaggtctt ttgcgtcaat
    36481 aaactggtaa ccaagatact cagccatcag ttgagcagtc agatattcac ctctacttac
    36541 taaataatct tcagttatct tttcgtcttc cagttgtttc tgtatgcgtg ctagttcatc
    36601 ttcaataggt aactgcaggg caagaacatc tcttacttga atgaaacgat cacaaatcct
    36661 tttccaaagg ggtgtccaat caacatgatg ctgaaggtaa gcataaatca aataaagtaa
    36721 gtctgttact ttgtcatcat ctgacgtacg tttgcctaaa gcagaaacaa cgatcacttg
    36781 gcgattggga tctgaaagaa caattgtacg gacttttttg aattgctctt catgagacat
    36841 cgatgttccg ccgaatttcg ctactttcag cgtattactc atcttgccac ctccgctatt
    36901 ttctgcatta aagctgcagc aatctgtacg gcattcgcag cggctccttt gcgtatgtta
    36961 tcggcagtcg tatagaacaa caatccgttc tctaaagaag tgtcttttct gatcctccca
    37021 acataaacca tatctgttcc tctagcaaga atgctcgtcg gatatttatg tgctgctaga
    37081 tcatcttcta attgaatccc tggaaactgg ctgagacact ctctaatttc ttccgtcgta
    37141 aactcttttt gcagttgaac tgccacagag actccatgtc cattttcgat tggcacacgt
    37201 acgcaggttg ctgatactgg caaagcaggc tgatgcagaa tcttacgggt ctcatttatc
    37261 attttctgtt cttcttttgt atacccattt tccaaaggct gatcgatttc cggaatcacc
    37321 gtttggctaa tattataagg atacaaaact ggttcctcgt cattttgcgt acgatgaaga
    37381 tcttcaatgc ctttttgccc ggaaccagaa actgcttgat aggtcgaata attgactcgc
    37441 tggataccaa aggcttcttg taaagcttta agcggaagta ccgcttgaat ggttgaacaa
    37501 tttggatttg caatcagccg attcaatgta tagtcttcca gattgacctc tggaacgatt
    37561 aatggaatat ccgaatcttc ccgccatgct gaggaattat ctatgacaaa tagtccctga
    37621 cttcgtgcga ttggcgcaaa ctctttggat acagaagcac ctgcagaaaa tagcgctaaa
    37681 tcatatcctt caaatgaagt ttctgtcaat tcctgtacag tgtgtttctt tttcttgaat
    37741 acacaaactt taccggctga acgttttgaa gctaataatt tcaatcgtcc tactggaaaa
    37801 tcatattctt ctaacacttt gagcatcgtt tggccgacta atccggttgc ccccacaata
    37861 caggtatcta tttcgcgcat ttttctccat ctcctctata attcgcgtaa cgcagcaatc
    37921 aatgctgtat tttcttttgt ctgatcatca cttattttta atacacgtgc aggtattcca
    37981 ccgacaacag tttctgctgc cacatcttct aaaaccactg ctcctgctgc cacgactgca
    38041 tcttttccga tatggacacc ttcaacaatc actgcattcg cacctaccag tacgccgtct
    38101 tctacgataa ccggtttggc cgatgctggt tcgatcacac ctgctaagac agctcctgca
    38161 ccaatatggc agtttttgcc cactgtcgct cgtccaccta agacagcacc catatcaatc
    38221 atcgtgtttt caccgattac tgcaccaata ttgataatcg ctcccatcat gatcacggca
    38281 ttgttgccga ttgacacttg atctctgata atagcacctg gttcgatacg agcattaatt
    38341 tttttaatat ctagcaaagg aactgcgctg tatcttgcag cagtttcgat ttcgatttca
    38401 atgatttttt ctgattcagc ttctaaaacc ggagcaatcg tctcatagtc accaaaaaca
    38461 acgaagctgc ttccttcacc aaaaacttta ctttctttaa attcgattgg ctcggttaca
    38521 ttcagataga ctttcacagg cgtcttttta gatgccgttt gaatgtagcg gatgatttct
    38581 tgagcttcca cgttttttac acccctttgt ccattaataa atcttctaat ttgtaataac
    38641 ctggtgactg atctttcaac cagtccgctg cttctactgc gcctgcagca aagattcgat
    38701 tagaaaaaga ttcatgtttg atcgtaaata tttcatcagt agcagcaaac aatacatcat
    38761 gttcaccagg taaagaacct gcacgaatac tgtgtacacc aatttggttt tcttctcttt
    38821 ttttcccttg ccagttgtat acgggctgca gcttttgcac ttcttggatt gtttgaagca
    38881 gcatatttgc agtaccagat ggtgcatctt ttttacgact atgatgtttt tcaatcagtt
    38941 cgatctgcca gccttgcaac attgcagctg cttgcttgat tagttgattc atcaccatga
    39001 tgccgaacga aaaattagca ctatacatca caggtacatg tttggcatgt tcttttattt
    39061 cttggaactg tgattgtgta taacctgtcg tgccaatgac taacggaagt tgatatgata
    39121 agctatacga aaggattttc tctaaattat ctggatgaga aaaatcaata agcagttctg
    39181 gcagttccgt aaaagcaagc gtagttgctt ctttaggtgc cgtccgtaaa agaaggaccg
    39241 tatctcctct ttcttgtgca acttcttcga ctctatttcc catctgtcca ctgccaatca
    39301 gtccgatttt catttttcaa ccgtcctttc ttttaacaaa agctgagcta attgtttctt
    39361 tttctctttt gaaagagaaa ctaaaggcag gcgtagaggt ccggaaggta agtctaataa
    39421 tgacatggct gctttgacag gaataggatt ggtctcactg aacaaggcat ggatcagttc
    39481 aagtgcattt aattgctttt cttttgcttg ctgccatctt ccgtcctgcg tatcttttac
    39541 taattcatgg actattttag gttggatatt cgcccataca cttattacac cgcttcctcc
    39601 tacactcatt actggtagga tcaaatcatc atttccactg taaaggaaga attcttctcc
    39661 aatcaaccga gcggcatcca tcacataagc catatctcct gaggcttctt tcaaaccaat
    39721 gatattagga tgttctgcta aatttactaa tacatggatt ggaatcgaca tgcctgttct
    39781 agaaggaaca ttatacaaaa taagcggaat cggtgaggct tctgcaattg ctgtaaaatg
    39841 agcagctagt cctgcatcac ttgttttgtt gtagtaaggg gtgatgacca aaagtgcgtc
    39901 tgcccccaaa gaagcaaaat gcttcgcttt ttcaatcgtt tctttcgtat tattcgttcc
    39961 agctcctgca atcaccggca cacggccagc cactttcttt actgtcagtt gaaggatctg
    40021 ctcctcctca gattgtgtga gcgtagaaga ttctcctgtt gtacctaaaa ctaataatcc
    40081 atctgtttgt tcatctaaat ggaattggat caatttttcc aaaccagcat aatctactga
    40141 tccctcttca ttcatcggcg tgatcagcgc cacgatcgaa ccttttatca tcttcattcc
    40201 tccaatattc ttgtccattc atgaactgct gggccagcca tttgtatcgc atcctcacca
    40261 gagatcatca agtctccttg ttccaagtga acaactacct gatctgctga aattttccca
    40321 gtttccttag caaccacata agaggcacag caacctgtac cgcaagcaag tgtccaacct
    40381 actcctcttt cataggtacg gacgatcaat tcttgttcat tgactacttc tacaaagttg
    40441 acattggttt tttctttgaa caaaggatga tgacagatca gttcacctct ttttctccag
    40501 acttcctcac gcgcattttc aacaaaaaca acggtatgaa tcgttcctaa aaataaacta
    40561 gtgatcgtta ctttttcacc attgatctct atttcctggt tttttattgg ataagtttga
    40621 ttagctttga ttttttccgg agaatcatct ggcactccca tatctacgct gcaataaaaa
    40681 ggtgagggat ccaacaccgt aatttttctt gtgcctgcta atgtctctac gttgaattcc
    40741 tgttcattca tcatgttctg atcatttacg tatcgagcaa aacagcgaat gccatttccg
    40801 cacatcggcg cgcggctgcc atctttgttg taatagatca tttccaacgg atcgattttt
    40861 acggcgatca atccgtctat atcatatttt tcttttgtgc ataaagaagc ggctaattga
    40921 ctataatctg tgtcttctgc gtaatctaat aagataaaat catttccgca gccattatat
    40981 ttatgaagga tcataggaaa cttcccccct tagtagatca gaaaatgctt gtctgcgaac
    41041 aatcagtctt gattggccat tattgacaaa aacaactgct ggtcgtgtcg ctcgattgta
    41101 gttgctgctc atcgagtatc cataagctcc cgtggaataa acagctaaaa gatcacccga
    41161 ttctgctttc ggcaaataag cttctttaat taccacatcg cctgattcac acatttttcc
    41221 tgcaatcgtc actttctctg ttttttcttc cgacagtttt gctgctaaat cacaatcgta
    41281 ttctgcctga tacagagctg gacggatatt atctgtcatc ccgccatcta caaagtaata
    41341 ttttttatgt ggtgtctttt tgatgaaacc aatcgtgtat agtgttgtac cagcttctcc
    41401 aacaatactt ctacccggtt cgatcagtaa ttgacgaatg gtcagttttc gtttttccaa
    41461 agcttcttcc gtatattcaa tcagtttttt tagtgcctct tcgactggga tcggctgatc
    41521 attttgtgta taacgaatcc caaaccctcc gccaagattc aaggaaagag gttggtcaaa
    41581 atcggttaag taattcgtca atttgtcgat ttcagctaac caagcagtaa catcaaaaat
    41641 ttgtgacccg atatgagcat gaaatccttc taactctaga tagctgcttt cttttatcaa
    41701 ctgaagggct tgttggcact ctttactttc atagctcatg ccgaattttg aatcaatatg
    41761 cgcagtgacg atatattcat gagtatgtgc ttctactccg acattcaacc gaagcatgat
    41821 acaaattttt tttaaatagc gttggcttaa ttctgctaat aattcaactt ccatcaaatt
    41881 atcggcaaca atatgtaaca aatcattttc aagtgcatag atcagttcct ctggcgtctt
    41941 gttgttacca tggaagtaga tagtatctct aggaaaatca gcttgcaaag cggtatagat
    42001 ttccccacca ctgacaacat ctagcccgaa tccatattca cgaactaaat gcagcatctc
    42061 aatcgtttgg aatgctttag aggcgtaaag cacttttgta tcaaaacgtt tcgatacaaa
    42121 gcctcgcttg aatgacagca tcgtttgtct catcttttct tcatcataca catacagagg
    42181 tgtcttatac ttttctgcca atgtgcttgc tgtgactccg ccaattatca attctgtcat
    42241 tttttcattc ctcttttcac tttttttccg tttttattgg aatttcattt gataaagatt
    42301 tgaattttat tggaataat
//
LOCUS       DAXWOO010000006        38229 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.006, whole genome shotgun sequence.
ACCESSION   DAXWOO010000006 DAXWOO010000000
VERSION     DAXWOO010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38229
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.006"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            13..861
                     /locus_tag="ACGE70_000261"
     CDS             13..861
                     /locus_tag="ACGE70_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFF8691932.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(1032..1373)
                     /locus_tag="ACGE70_000262"
     CDS             complement(1032..1373)
                     /locus_tag="ACGE70_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFF8691933.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SMEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            1459..2166
                     /locus_tag="ACGE70_000263"
     CDS             1459..2166
                     /locus_tag="ACGE70_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFF8691934.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            2153..2476
                     /locus_tag="ACGE70_000264"
     CDS             2153..2476
                     /locus_tag="ACGE70_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFF8691935.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(2565..3602)
                     /locus_tag="ACGE70_000265"
     CDS             complement(2565..3602)
                     /locus_tag="ACGE70_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFF8691936.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(4049..5032)
                     /locus_tag="ACGE70_000266"
     CDS             complement(4049..5032)
                     /locus_tag="ACGE70_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFF8691937.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            complement(5448..6218)
                     /locus_tag="ACGE70_000267"
     CDS             complement(5448..6218)
                     /locus_tag="ACGE70_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8691938.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(6211..7146)
                     /locus_tag="ACGE70_000268"
     CDS             complement(6211..7146)
                     /locus_tag="ACGE70_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8691939.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(7740..9389)
                     /locus_tag="ACGE70_000269"
     CDS             complement(7740..9389)
                     /locus_tag="ACGE70_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFF8691940.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(9584..10480)
                     /locus_tag="ACGE70_000270"
     CDS             complement(9584..10480)
                     /locus_tag="ACGE70_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFF8691941.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(10473..11153)
                     /locus_tag="ACGE70_000271"
     CDS             complement(10473..11153)
                     /locus_tag="ACGE70_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFF8691942.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(11154..11936)
                     /locus_tag="ACGE70_000272"
     CDS             complement(11154..11936)
                     /locus_tag="ACGE70_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFF8691943.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(11929..12846)
                     /locus_tag="ACGE70_000273"
     CDS             complement(11929..12846)
                     /locus_tag="ACGE70_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFF8691944.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(12849..13133)
                     /locus_tag="ACGE70_000274"
     CDS             complement(12849..13133)
                     /locus_tag="ACGE70_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8691945.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            13980..17555
                     /locus_tag="ACGE70_000275"
     CDS             13980..17555
                     /locus_tag="ACGE70_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFF8691946.1"
                     /translation="MISNKKRWLKVALLVAFCQIAFLASMTTQAEEIVQETTVSQVSP
                     TSNEVEVQSTLPKEAVKEETPSEVVPETEAPSTNEKTVLDETIGKSQQPGDSPVVAEE
                     TPVEAHEAQAIPEKDKSLEESGSTEINEGTEPVQLEDSKEVTTPIESTKEEMDSNTSL
                     SSEKEEKTVLLTEPREPELKKVEEFVDTKKEAVSPTSSKEDTLIVQQTARSARVRRAT
                     NTAVLGGRKIDWGENIGVISSANNPNFTLNYMVRFEINGQVAFCIEPGVAAAEGQVYT
                     ATALENYLKDLNIRKKTSLISYLGYVTSKDKSDEQYIAAQFMIWEVLGTKIPIKNIRI
                     DYDRRKAEIQRLLNNFDVRATFHNQTHTVKIGETLKVKSGNDFLQRVKDIQKPKGVNV
                     SIHDNEVWITITKESPEKAKIHFNQIEALGVPLAYRRPGAQTIGVLNPFVPGDSVVNL
                     TILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQANGKKIKEITLKNINGKVQAEI
                     KDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNTIAKDQVIKGTIDLVKVANKD
                     LVDSTNPDNKPKLAGIEISLTSKTTGKVVKTVVTDNDGYATFGKNTVVFDTYILSETK
                     GKEGYKLFEPFEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKVIALAGTQFKIW
                     DKYANNGKGGYVSMALPNDTEVSDIFTTNEKGYFVTTDTLKYGKNRYELREIKAPEKY
                     VLNKTPYVFSVTDDTNTIQLIYFANRLAKANVEVFKYDTSTNFDQKTAIAGVEFDLFK
                     KDETGTTFVGTFKTDKKGKINVKDLLVGDYFFKEVKPLAGYLALDKDVPFTVTVDKDG
                     TVIALEVENKRIPPKIGTTAIGKYTNQQKVTPLKKVTITDTVKYSNLIIGKTYTVNGV
                     LMDKEESITAGKDVPLLVNGKQITASKVFVAKNPNGMISLDFVFDASALAGKEIVVFE
                     NVYRDGRLVASHTDIHDKGQTVRIVNPMIHTKATGQNGEKQLQALPNQKVKELVQLND
                     LIVGQTYKVLVQGYITPDGVKLDGTSAEKIFKADKTTLEFLFEFLVDGRKLAGKGLSF
                     AEWLEAQSEPKDENDYEVRFEQIANHNVDLKSEDQTVYFVEVPKVPEPKIPEKSQPFK
                     EVKQASVVPMLPKTGSEDTVLWMIIGVLFVLAAGLTFYELRKVKD"
     gene            17655..18593
                     /locus_tag="ACGE70_000276"
     CDS             17655..18593
                     /locus_tag="ACGE70_000276"
                     /inference="COORDINATES: protein motif:HMM:NF012948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFF8691947.1"
                     /translation="MKKKERKKRMKKLFFACLFSSVVLAGGINVFADEIGITPVTPPV
                     IEETTPVVPIPDISKIDESVPDQPTPVDPDISSEVTEPTTPVVPDITENSENETPVVS
                     PSTGETTNDKDTTPTTSESYSGTSSVDNDSKIDDFSSSSTTIDTPKETEKKNDLGAPK
                     NLTPENHIKTVSEKEMTVSVSSDGKIVQGTGTEKSIPIVTSNLEEINHVPTSETPLQV
                     ATGQAIVGVQDGVPLVQDNQGNLVEDLSIPVKKLPSGNIEIKTIDGKTKVLPKTGEEL
                     QISLTILGSLLTAVTGGIFYGKKQWRHLRHMFKKTI"
     gene            18612..18962
                     /locus_tag="ACGE70_000277"
     CDS             18612..18962
                     /locus_tag="ACGE70_000277"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8691948.1"
                     /translation="MGLSYVAISQEEVLGKCFNQKHVGSTGERDRRGNMKKVEWEIFQ
                     MNSEKMGEIEVRVAVGGAPKAMRYNAEVLLANPRISRRADVRNIGNDGETNKIARNRF
                     YLLVDRVEVKGGNN"
     gene            18962..19393
                     /locus_tag="ACGE70_000278"
     CDS             18962..19393
                     /locus_tag="ACGE70_000278"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF961 family protein"
                     /protein_id="HFF8691949.1"
                     /translation="MTLEFDQGIVPGLVKETLGEIYFVKAQRPRMLYEDNENTGEIKE
                     RPVEFSSATQETMFVVNFPPKTNLDGLSFGDVVELEEVEFRFWISIDRESMSNRAEND
                     LKVSALRYKKTGKNVFQTSSSTRATYVKEKQDSNKESAKKE"
     gene            19661..20980
                     /locus_tag="ACGE70_000279"
     CDS             19661..20980
                     /locus_tag="ACGE70_000279"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFF8691950.1"
                     /translation="MFQYKGIRIRNRHKYASERFIFYLMLPVLLLMSGFFSLFYTTIT
                     WEWCCIYWIIAFGLAGLFAYMCLYFYFYNVYQRLVHKGKLAKMIHSRRLILERVLKNG
                     RRKVVYYPKIFYHYKNGLIHVRLPLDLGPFQDRFNEMAGQLEEAFFCDLVEEQREKNY
                     ICYTFLYDVEKNRIGIRDITVKDGIITLMKNVQWAFNKAPHAIIIGSTGGGKTYFIMS
                     LIYALLPYGTFDICDPKRADLASLEKIPIFKDQVFYGGGILSCLINLAKETANRYDFL
                     REQPEYELGYDYTYYHLTPHFLVIDEWAAYYASLDTKEKKKAMGCLNQIALMGRQCGV
                     FLILGTQRPDTKYLDGAIRDNFGLRVTLGRVSKAGYKMIFEHTVKSLFNKPIKGRGYI
                     DSGTDQVREFYAPFIDITRFNFFAEFKQLSDQLMAEKIKETKEGKNV"
     gene            20973..21137
                     /locus_tag="ACGE70_000280"
     CDS             20973..21137
                     /locus_tag="ACGE70_000280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8691951.1"
                     /translation="MYEEIPATCGTRLEIKDLMIDSFLAIVFSEKRLSFFINKKVENR
                     WRLERMYPTS"
     gene            21676..22851
                     /locus_tag="ACGE70_000281"
     CDS             21676..22851
                     /locus_tag="ACGE70_000281"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFF8691952.1"
                     /translation="MDGTQLKQMRLSRNLTQKLVADTLGWPQSFLSMIENDQRSCSKE
                     QEEALCEFYANFSSFTSLEIKFDYVRVRVPTNDTKTVVEEILGMDFNSFFYAETRLYG
                     YIEMYQKGMIRVLNSKKGDDKGVLIELSGQGCRNYESVLDERNDSWYQFFTRCVSFNG
                     VPRRIDIAVDDFEELFSLHEFAQKLNRGEFDSKFRTWRFQEEKQLLDNLSSGLSVYLG
                     SSQSLMYFCFYQKNYEIAKKQKLPLEEVEVKNRYEIRLRDAKAVSFIEQYLNKVDFSD
                     LTMGILANQLIMFEGEKNANVWKPWGKLIQNVGKVNLTITPEKPNYNRKLMYLKTQPA
                     RTMKIVKTVDEILGLNRFGEILEETELREQDEKIVEDEVRAVYEYIQENPDAIHYLL"
     gene            23356..23553
                     /locus_tag="ACGE70_000282"
     CDS             23356..23553
                     /locus_tag="ACGE70_000282"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8691953.1"
                     /translation="MGQYFLTGVWYVLKLVIILGIAYICFRILLSGEGMPTPKDFIIS
                     VLNFVADQIQVIIHWAQQRFG"
     gene            23595..23723
                     /locus_tag="ACGE70_000283"
     CDS             23595..23723
                     /locus_tag="ACGE70_000283"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8691954.1"
                     /translation="MIENFEYKLSRLPKKKGATQKELANKLGVNVQSVSNVEEDKS"
     gene            complement(23949..24497)
                     /locus_tag="ACGE70_000284"
     CDS             complement(23949..24497)
                     /locus_tag="ACGE70_000284"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8691955.1"
                     /translation="MAILQPFGEPIERTEFIQHYMKLFVQVIKHTHQIDEFYSKEIEY
                     LLAEKQKIALLYDYFVEMYDRAPDYFYLSDTLTTNFLAKEYLFASHTKNFMCVEHFVN
                     TYLHLLKTQKICTFEAFQTDYLFILDREAYHAKQAFEKQNQAIEGYPELRIQNNSFLQ
                     QRLLKQLINGFHQRNKGYQKDQ"
     gene            complement(24552..24845)
                     /locus_tag="ACGE70_000285"
     CDS             complement(24552..24845)
                     /locus_tag="ACGE70_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8691956.1"
                     /translation="MLRTNAIPIVDSYDRNTNNYLGSFEQTDENILNYVAGLSPFQSV
                     RLVEHTTDTLILTTIGYFFDQVSDQQWLQQILPKLIAKQTGKKTIEAVKIFFY"
     gene            complement(25045..25386)
                     /locus_tag="ACGE70_000286"
     CDS             complement(25045..25386)
                     /locus_tag="ACGE70_000286"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8691957.1"
                     /translation="MKANTPYKVGDIFYSSWGYEQTNVNFWQIIKLTEKTAWFRPIKK
                     QTVKTYTSMSGETMPIPNEFIDYPLVRTKDSIVRKRINPALYPNELYGNRSWDTFYRY
                     DNQPVYESSYY"
     gene            25887..26036
                     /locus_tag="ACGE70_000287"
     CDS             25887..26036
                     /locus_tag="ACGE70_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8691958.1"
                     /translation="MKKKVIPYCPKPKNAFERGLFKYFEGKKGQDHKEENQKKKSKKK
                     SEEFF"
     gene            26311..27111
                     /locus_tag="ACGE70_000288"
     CDS             26311..27111
                     /locus_tag="ACGE70_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFF8691959.1"
                     /translation="METIIQIMKKYDFSEMETRVYTTLLEKGNLTGYEVSKISGVPRS
                     KVYNILEKLLKKNLIVVNKSEPKLYHAISANEFLEKLEKSVKNDLSFLTKNLGMIKEK
                     DEEEMLWKVDGIEYVLDKVEHLVKNAKESLLIQVWHENLTDSLLKTLQQAEKRVDKFV
                     LILFSSTHEYDLPLEKYYIHGFETDKLADFGARWINIVADEQEVVFGTINEELQSTDV
                     TWTKNHAMVNLAKEYVKHDAYTLKVIAESSEELKAKYGEDFEGIRKIY"
     gene            27130..28005
                     /locus_tag="ACGE70_000289"
     CDS             27130..28005
                     /locus_tag="ACGE70_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFF8691960.1"
                     /translation="MRNILLTLIVAINLYFIIYFLRDLFKNKQNFKEEPGDMRLLPFT
                     SFIIFFLSTFGVSDFAIGTVLYPKLKWVSMKKLPGTLNTQCVVPVAVMALSYITAINV
                     GIKTLAVCIICQIIGAYLGPRFVVKLPEKTIKVFVGIGLVIAALLIFAGQMNWIPSNG
                     TASELYGGKLILAGFLLFVYGALNNIGIGSYALTMVTVYLLGLNPVAAFPIMMGACTF
                     SVPIGSVQFIKFNEYSRKITLFTSTFGVLGVLVAVFLVKSLDTYVLKWIVILVLLYSA
                     YTMLGSQLKKVSAAN"
     gene            28018..28998
                     /locus_tag="ACGE70_000290"
     CDS             28018..28998
                     /locus_tag="ACGE70_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFF8691961.1"
                     /translation="MLFLKKEDIIKSFSMREAIDADKKALSLYSAGKASVPLRTNIDV
                     PKSNGQSLYMPAYVEGGEGALGVKIVSVYPENIKKNLPSVPATMIVLDPETGMVSACL
                     DGTYLTQLRTGAVQGAATELLAREDAKIGALIGTGGQAQSQLEAMLTVRKLEEVRIFD
                     IDFDRASRFAEEMMQQFSVTMRPTKTSQECVEGADIITSVTTSKRATFSAEWVKKGAH
                     INGVGAYTPEMCEIPREIIKAADVVIFDTMDGVLKEAGDFISPLQDGYIQRDSYHGEL
                     GQLINEELIGRTSGEQITIFKTVGSAVLDVVVATEIVNKAKENNLGKLIY"
     gene            29520..29738
                     /locus_tag="ACGE70_000291"
     CDS             29520..29738
                     /locus_tag="ACGE70_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8691962.1"
                     /translation="MNFFQNVFTDFVGDFKYLIMLAMLGGAAYIAFERKVSKAIPLIL
                     VLAVAVWLVGDTSGVFNWILDRMRSWGR"
     gene            29740..30099
                     /locus_tag="ACGE70_000292"
     CDS             29740..30099
                     /locus_tag="ACGE70_000292"
                     /inference="COORDINATES: protein motif:HMM:NF024062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFF8691963.1"
                     /translation="MNKIRNWTKIWYIERVFYKLWDGVALPRPVTQTFAIWFGIGFFG
                     GIFRIFPFVMEHTITRQFFCPWLFAFLMTRKQFQGRKPYNYLASFLLFQCRSKESFHG
                     QWVKPIEKKKSNEEFVV"
     gene            30121..32658
                     /locus_tag="ACGE70_000293"
     CDS             30121..32658
                     /locus_tag="ACGE70_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFF8691964.1"
                     /translation="MRLPKLVPKIPFYYLEDNLVFGEEDRVYAYYEFPMYSYSFVGED
                     QAYQLQQAIMRLIRQSYSSHVRFFFCTNEEKIEKTIKASKELVRSEGELKTIAYQHLE
                     GVQHILEELHGKYIQNVCWYVGIELVLEESAPKEAHWMKEFRIATKDFFHRSNARLFG
                     DFETIPNEKIQRYKRLENLLYNRISKYFRLRKTTPEDMVFLVEHLEGKKEQSLDEVMY
                     RPNSFKEETNTYTYHYDVLNLASAGITEKKNYLILEQGTEKEYITYLSLSRMTRDLVF
                     PFQSEIYYFLQSELDFPIDSWLDVNLLDNRLALAKVRGKKSDLSDIYESGMSVGKDVG
                     NNVREAYQSANELEQKLEDTREDLYVLSLNVRVSGKTYDEMLKRVSDVRNFFDVYSMY
                     LEVPCNVQLFLHFGSYPAGRRVIEDYRQYVGAEFIASLGIGATQQLGDGCGIPIGFNA
                     ETGIPVFIQPWLAAQGIKGTVTNTLAKALIGSLGGGKSLTENLLLFWSVLFGALAFCI
                     DPKSERSNWSEDLPYFAPYLKTVNITSEKENIGLLDPFQLMRRKKDQESLALDVLTYI
                     TGVSIRDEDRFPLLQRAVKTVSDFEGTKGLLLVIQELYAQEAAIAQRLAKHIESFKEL
                     SIAGLLFGDGTNQQGLNLDAKCNLALVQDLTLPDSETAPEDYNSSEILSIAIMMSLAT
                     YALDFIKQNRDIYKAVGLDESWAWLNVAQGKILGNKLVREGRSRNAGIDFSTQNTDDL
                     GDEKMRSKIGLKFIFRSNDREEITKALNFCGISVTEENIQRVKNLENGECLFCDIHGN
                     IGILYVYYWFEDLFHAFDTRPPMEKNYEEEEYKDEILEKLVEEIEVN"
     gene            32659..34680
                     /locus_tag="ACGE70_000294"
     CDS             32659..34680
                     /locus_tag="ACGE70_000294"
                     /inference="COORDINATES: protein motif:HMM:NF046089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFF8691965.1"
                     /translation="MKQWYSKVSFSITRRKLVFISLVIMMGVFFFHPIFVEAAGWIKE
                     TLGGKNAVYDFETYPLDNYNLDFFVDTSWNWLPWKWGEGISNTVIYGIFAVTNILWML
                     NVYLCYFLGFLVQEAFELDFLSELIGSLSHSIQNIAGMNRAGTLSHGLVPFFGGLLIA
                     LAGCSVVYQGIVKQHPTQAIQQAVTFLFTFICCGIFFMNTHTYLEKANEVQKEINSEM
                     LTIAKDLLPEQKGKPDAIKAIRENLFAIQIKQPWLVLQFGDSDIKTVGEERVINLLKE
                     SPYDEGEQRNKLVEEEVTKQKNGNLSVQKVYLRFGMVFLTLIVNIVISMGVLALVVIL
                     IASQVLFLLFIGFLPVAMIFSLFPNSGSLLGNALQKVFQLLFTKMGILLILTITFSIS
                     HELNHLSKNKGMIWTSFLQVTLWFSVSTRVNELLGWMKIGGAQTRAGDRTGRFLRGMM
                     LGTMGQKMIRGIGENFVGGAAGGVADQMISKSMSGQRSITTTPLIERVGKKVSAMTEL
                     PATIESRAEQWKRTIQYVPTNAQYKAQKMKYDYFSGRMNESERQTRARNLANERQKNF
                     YEARKDALQLEQQERKQQAMRSMAHQTGKTQVTSGKLQTRKSAMIGRTEQFPTIEKRS
                     NISNVAKNKKLSKQEHPIHERSTNRKIETPVRWTSTSCQNARKGRTKKE"
     gene            34827..35615
                     /locus_tag="ACGE70_000295"
     CDS             34827..35615
                     /locus_tag="ACGE70_000295"
                     /inference="COORDINATES: protein motif:HMM:NF024056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFF8691966.1"
                     /translation="MVFSVCFGVYNNLTSQDTHTIHETKVIDKQLKDMTGIESYVKNF
                     AFLYFTVSLESDQQINRSKAVESYLADGLQMDNEIDKDIETNVKVKNVSIWAIEEHES
                     KKHTFVVIFHVLLEIEKEFKERVYKVEVYRNELSYAIVKLPIVASNVKKATIKVDTRF
                     ETTSISRKEQEELEKFLYTFFRIYPKANAEELKYYGTTLLPIQQPLAFEELSNLQVAK
                     KEGRYEIHCNILYQDQETHLWFTMYYEMSMKQLENGNFRIEAIH"
     gene            35651..36706
                     /locus_tag="ACGE70_000296"
     CDS             35651..36706
                     /locus_tag="ACGE70_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002386462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HFF8691967.1"
                     /translation="MSCLFLLFAIISMSSGESKHMDSAVGVTHTGEYIETLPIFSEIK
                     GRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRNDTNFFGITWFPGC
                     PYPQGSARGIGGSEGGFYMKFPNLNACYDYYGFMVASQSNFNQCVGNKDPGSCLLILG
                     RGGYAAAGITEGSPYYQGAMSIIKSYNLTEYDAFAIKHWNSLPTKKTSLIAGKSKGNA
                     KVLDRIIGRPVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGEDYNWATYGWQVIM
                     NPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSNSGQSFSTVEQNAEQGRIMARY
                     QRSYNQAKIRSLVRKVK"
     gene            36757..37269
                     /locus_tag="ACGE70_000297"
     CDS             36757..37269
                     /locus_tag="ACGE70_000297"
                     /inference="COORDINATES: protein motif:HMM:NF042930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF0163 family protein"
                     /protein_id="HFF8691968.1"
                     /translation="MKNYYFFFLSCFCLFGLTSCGWIFSEEKTEVSLISKEKMIQAES
                     SSKESKRNQEDKTECNISEEIQYLLKNFGRKWVNYTDIYERNQSVKNYLTDRCIKENG
                     IDNDPHVQYKTIGRIHTITQDVIQSDQYLLFGEETTNEMTRFVLLQIKVDQEVQKIDK
                     IKIYYVRSAY"
BASE COUNT        12509 a         6513 c         7437 g        11770 t
ORIGIN      
        1 aaggagaact agatgaataa ttggcttgag tattttcctg aaaacgtttt ggaacgcggt
       61 tacagctatc atctgcacgg ttttgtccgt catttgaact atacaagcaa gtacttgtcc
      121 gcaacagttt ctggcacgga agattataag gtagtgatca cttgggatga aaagaccaac
      181 atgacttgtg attgtctata tgccatagaa ggaaaaaaat gcaaacacat ggctgctgtc
      241 ttattcgctt acgaagaacg tccaataaaa aaatccaact actcactttc cgaactctcg
      301 tcccttgttt cctccgctag ttcctctctt gtacgggagc ttttaacaga gattcttata
      361 gaacatccgc actttatcga acgcttcaaa gttaagatgc catttcatgc aataaattat
      421 tcagataaat taacaactat catccataaa tacgatcata ttatcaagaa aaacaaaaac
      481 aggaaaacag cgaagttcat catggagatg cgaaagttca tccaagaagc agttgaatcg
      541 cttattcagc aaaatgccta tcttcccgca ttcgaattga tcaatgaagt gattgctact
      601 ctcgaaacat tctattggga gcctgaggat gaacgaaccc ttcttctgat agaggactgt
      661 tattatttat ggaaagaatt attagcagaa gcacctcatg cagaaaaaag gcaaatgttc
      721 tcttggttcg tctgccaagt cgatcatact gacgcctctt attcaaaacg ctactctata
      781 aaaatcctga aagaagactt tcgggaaaaa gaattttcta accaaaagaa aaaaatagat
      841 aaaaaactaa agaaacggta aagaaagacg atttcaatga aaaatagcaa gtaaacgata
      901 acaaccatcc taaatccaca agaaatacag cagaccttgc tttaaaaaca cgaatcatgc
      961 aaaggatgaa gtaaagaaga cttaacgcgt acccaatatt agtcatttga ctattaggta
     1021 cgcgttttgt tttataagtc atgacaaatt tccttcgttg ctggatagat tttccggcat
     1081 tgttgataga ttttatgata cttttctaca ttttgtaaga gaggttccgc tgcatctgta
     1141 tattggataa aattctgaca acaactttca ggtgtgtcga accaccctaa ccctagtgca
     1201 gcaatcattg ttgcgcctaa tgcaggtcct tcctccatac ttaaacaata gaccgttgca
     1261 tcgaaaatat cagcttggat ttgtagccat gcaagatttt tcgcgcctcc tcccaccgaa
     1321 acaatacgtg taaatctgcg acccgctagt tctttacgtt tcaatcccac cattgacaaa
     1381 tgctcattaa aaatttagaa tattcacaag tttaacgaca attatgaaaa tttatgtcgt
     1441 gctaaaggag ttttgttatt ggatgaaaag ttagatataa aaactgcgat caaagacacg
     1501 ttacctaccg ttttcggtta tatcggtatt ggacttgcat ttggtatcgt tgggaaagct
     1561 gccggatttc atccactagt cgtcacgttg atgtccctgc tggtctatgc tggttctgcc
     1621 caatttatca cagtcagcat gcttgctagt cacagcccat tgctttccat cgttttctcg
     1681 acctttctag tcaattcccg aatgattctg atgagtatga cgattgctcc ttatttcaag
     1741 aaaaatcggc tgcttcaaaa tctgctgatt ggcacattgc tgacagatga aagtttcgca
     1801 ctgggaatga ataaattgaa ctatactgga caaaagttaa actttcgttg gatgaataca
     1861 gctaattgga tttcttattt gacgtgggtg ggttcttctc ttgttggcgc acttttaggg
     1921 aattttatta ctgatccgaa aaagtttggt ttggatttcg ccatcgtcgc tatgtttatc
     1981 gggttacttt atcttcaagt catttctgat agaaacacct ctaaacgatt acagttgatc
     2041 ctgattggtc tcactttgat tctggtatat gtcggactga tttttatccc aagcaactta
     2101 gtgattgtag ttgtaacact gattgggtgc ggattggggg tgtggatcaa acatgccttc
     2161 ttttgagttt acattgctga ctattttagg ttgtacttta gcaacttggc tttctagggt
     2221 cctaccattt gtcttactga aaaaattcga tttaccgcaa ccgttgcttg agtatttaag
     2281 ttttgtcccg atcgtcatta tgtctgcttt atggttcagc agtctattta cccaaaatat
     2341 cggacatctg ccgcagatca atctcgaaaa tgctttggct tcagttccta cacttttagc
     2401 tgctattctt tcaaaaagct tattggtgat cgtcttggca ggaattcttt ccctttcgtt
     2461 gattcgctta ttataaataa aaaaacaaga gcttatggca aattactgtc atgcgctctt
     2521 gtttttatca aagtcaaata agaaaaaaat cggcgtattc ttgcttatcc gttgaacgga
     2581 ccactaacaa cgacttttcc taacatatgt cctgtttcta cgtctttggt tgcttgtttc
     2641 aagttcttcg cattcatccc atctgaatag accttgctca atgtactgcg gataatccct
     2701 tcatttgcta aagcagcaat gtgcgctaaa gcttctcctt gcgtttcgat ttcatagtta
     2761 tagtctgttt tagcgaacat atactcccag tcgaagctga tagattggtt tttccaaaca
     2821 cctatatcta acggctcttt gatccctacg atcgttccca catgtccaaa tggtcggaca
     2881 aggtgcttga tttgatcaaa gtagcgggta atatcgaata atacagcgac atactctact
     2941 tgatcgattc ctaattcacg aaaactttta cttaagtcgt gatgataatc gattggatag
     3001 tctacacctg tttttttcag ccattcaaaa ttcttcggac ttgcagtcgc gtagacttct
     3061 aggccagccc aatgcgctag ttgattcaaa atcgaaccga cgcccccgga tccgttgatc
     3121 actaagattt ttttcccgcg attggcattt tcttctggag ttagaccaaa tttttcaaat
     3181 aacaattcat aagcggtcaa tgaggtcaac ggcaaggctg cagcttcttc atctgaaaga
     3241 ttttttggtg caagagcgac aatacgttcg tcaactagtt ggtattcttg attgcttcct
     3301 gctcttgttg ttgtacctgc gtaaaaaacg cgatcgccga cagaaaattt ttgcacttcg
     3361 tcacctacag caacaacttt cccgactcca tcaaaaccta agatacgtaa agcatttctt
     3421 atcccgttat cttggcgaag tttcgtatcg acaggattaa cagatactgc gttgactttt
     3481 accagaagat cccgtgaact gggtacaggt atctgttctt tttcatcaat aaagcttttt
     3541 ggatcatcga tcggcaaccc ttcataaaaa ccgactacct tcattaattc cttttctgac
     3601 atacgaattc ctccttagct tcatcaagtt ggagcatcca tacagtgttc atttcaatat
     3661 atatagtatg ctatttgttc cacctttttt attcatcata attattctct tacttaaagt
     3721 aaactatttg cacttttttg gttaaaaaag aggttgcgac aagacttttg tcacaacctc
     3781 ttataaacgg tgttctccta ccaactcttc ggtcacaagt caaaatattc atcaaacatg
     3841 agaagctgtt ttttgaaatt gctccttgtg actcagagtt aaacggcagg ctcacaacct
     3901 ctgatataaa cggtgttcaa tcaactgacc ctcggtatta gctcaaaaaa cgacaaaata
     3961 tgaagagcta tttttgtttg gctgttctta ttacttgata caggacagct ttttcacgac
     4021 ctctaattca cggtgttcaa gcaagaaatc attgttcact gaaaagcgtt ggatgttctt
     4081 tttcaaaggt ctctaagatc tctcgtactt catcagtaga tttcgtattc atcaactgaa
     4141 ctcgcaagtc atttgctcct ggaaatcctt tgatatagat tttaaagaaa cgatgtagtc
     4201 ctgtgatgga acgcgggatg atttctgaat aatgatcttg cagatctagt tgcagacgaa
     4261 gtagtccgat cagttctttt tctgtatgtt ccctcgggtc tttttcaaat gcataaggat
     4321 tcttgaaaat ccctcggcca atcatgatgc catcaacgcc atatttctcg gccaattctt
     4381 ctcctgtctg gcgatcgggg atatctccat tgatcgtgat tagtgtttgt ggagcgattt
     4441 ggtcacgtaa agcaatgatt ttaggaatga cttcccaatg tgcatctact ttactcatct
     4501 ctttacgcgt acgcaaatgg acagaaagat tggctatatc ttgatgaagt aggtgactga
     4561 tccattcttc catctcagac atttccgtat agccgatacg cgtcttgacg ctgacaggca
     4621 aaccgccagc tttagctgct tcaatcaatt gtgccgctac ttctggacga aggatcaagc
     4681 cactgccttt tccacgttct gctacattag ggactgggca gcccatgttg atatcgattc
     4741 cttggaagcc catttctgcc atagcaatgc tcatttctct aaaaaattct ggcttgtctc
     4801 cccaaatatg ggcaaccatc ggctgttcat cttctgtaaa tgttaatctt cctcgtacac
     4861 tgtcttttcc atctggatga cagaaactgt ctgagtttgt aaactcagta aagaagacat
     4921 ccggtgctgc tgcatgcttt acgacatggc gaaatacaac atctgttaca tcttccattg
     4981 gtgctaaaac aaaaaaaggc tttggcagct ctttccaaaa attagtagtc atttatttgt
     5041 tactctccat tctatcaagc agtgaagtcg caatctttct caaaaacatt gtcattatac
     5101 taggaaacaa aaaaagaagc aaagtgaaca gcttaaaaga tgtttctgat tgagcaaaaa
     5161 aaagactgtg acaaaaatct tatcacagtc cattttccgg aataaatggt gttcaaaagc
     5221 ttgcatttgc ggtaataagt ccaaccaaac gaaaaatagg aggagctatt tttgtttggc
     5281 tgttcttatt acttgatgca gaacagcttt ttcacaacct ctccactaaa cggtgttcaa
     5341 tcagctgacc ctcggtattc gctcaaaaaa caacaaaata tgaggaacta ttttcccgtt
     5401 tgtttctcta atactcaggt caaaacgctg atttcacaac ctcttttcta acttctcaat
     5461 cctcttcctt tattgatcaa ataccaagta acggcgtaca aaacaacgat gaataacaca
     5521 agtacgatca tcgatgttgc tactggcaca tcgatcgttc caaggaatcc gtaacggaaa
     5581 ccggagatca tataaacgat cggattgact tttgaaacag cttgccaaaa gggcggtaac
     5641 atagagatgg catagaaaac gccacctaga taagttaacg gctgcaagac aaatgtaggc
     5701 acgatcgata catcatcata agactgagca aaaatcccgt tgagcagtcc tgctaatgaa
     5761 aacaagattg ccgtcatcaa taatgtaatg atgaccatga accatgaata cacatgaagt
     5821 ggtacaaaga acaaggaaat gatcgttacg agagagccga cgagaatact gcgacccaca
     5881 ccaccaatca caaagcccca aataatcaca tgcgtaggaa caggtgctac caagatttct
     5941 tcgatatttt tctgaaattt ttgagagaag aaggacgaag aaacattggc atacgaacta
     6001 gtgatagcag acatcataat caaaccagga acgatgaatt ccatatatga aaaaccaccc
     6061 atgtcaccga tccgcccacc gatcattttt ccgaaaataa caaaatacaa ggatgtcgtg
     6121 atcactggcg gaaccagtgt ttggacccat atacgcaaat agcggttcgt ttccttgata
     6181 gctaaacttt ttaatgcagt aaaataaaga ttaaacattt ttctcctccg tatgattatt
     6241 ttcttctgtg attttcaaga acagttcttc taaacgattc gacttattcc tcatagaaag
     6301 aactttgatt ccttgtcttg taagctgatc gaagattccg ttcacccctt gatttcgttc
     6361 aacttcgaca gcgattgtct gctcatcttc aaatgtacaa gcatatcctt caatcacagg
     6421 tttggtatca tatggcgcta aatcaaagat aaatgtttca aattgcagtt ttgacagaag
     6481 ctctttcata cttgtattct cgatcagttc acccgattgg atgattccga tgttgcgaca
     6541 aagcatttca gcttcttcta gataatgcgt cgtcaaaatg atcgttgtac cattttcgtt
     6601 caattctcgt agaaactccc acatctcgcg tctcaactcg atgtcaactc ctgctgttgg
     6661 ttcatccaag atcagcaaac gtggttcatg catcaatgcc ctagcaatca tcagtcttcg
     6721 cttcattccc ccggaaagca tacgtgcacg gacatttctc ttttcccaaa gattggactg
     6781 cttcaaatat ttttcgctgc gtttcatcgc ttctttgcga gagacaccgt agtagcctgc
     6841 ttgattcacg acgatttgct ggaccgtttc aaaagggtta aaattgaact cttgtggaac
     6901 caatccaatc tgttgctttg ctcgaactag atccttgtcc agatcataac cgaagacatt
     6961 cacttttcca gatgttttat tgactaaaga agtaataatc ccgatcgttg tagacttccc
     7021 agctccattt ggccctaata gcgcatagaa atctccttct ttcaccgaaa gatcgattcc
     7081 ccgcaatgct tcgaccccac ttccatagat ctttttcaaa ttatttattt caagcgcatt
     7141 tgccatttat ttcctatctc cttatagaca tgattcttaa ttgtatcatg gaatacagca
     7201 gatgaaaaaa caacttggct tccacacaaa aacacacctg ctaacaaaga ggagatgtgt
     7261 tttctagttc atgatcatct tcgatagaat aactatctaa acaactctgg aacttttaaa
     7321 atcataaaag taccgacagc aactactacg atgacatata gaagatcctt ataactgagg
     7381 ttcttgtctt ttttattcat gttaagcttt cctccgtcct tttatcgaaa gcttttttct
     7441 tttattttaa ccgaaataaa taaaatggaa atagttattt tcaatttctg ttttcgctgt
     7501 ttgttttacg gagaactttg ccatggaacg tgcgtctcgt cgctgatgct aagttttttc
     7561 gataaaagct gctagaagga agacagcgtg gtttattttg tatcgtcttt tgctaaatct
     7621 tccaagatca tctggacagc agactcgttc ccattgaatt ctgccacttt tttggcatca
     7681 atgtacttct tttgataatc gtctttttgg acccacgcat catgcttcgt ttgataaagt
     7741 taatcaaacg gataccactg acgctgttca tcgaacaaca aactttcttt ttgggcctgt
     7801 tcaactatgg atttaggata attcaaaact ttcagcaagg caatcgcatt tcgggtcctt
     7861 gaaggtcctt cttttaattt gaaatcaaaa ctgattcctt ttccttctgt tacttgttct
     7921 tcaaagtgga cattctcaca ataattcttt aaaatctctg tcaattcgat atcatgtgtg
     7981 gcgacaaatg ctaaagatgg atactccgca agccaccgaa cgactgaagc ggaagaagca
     8041 atccgttcga tcgtatttgt tccttttaaa atctcatcta cgaagcagta gcagcgttct
     8101 ttggaagcca cttggtccag caaacgtttg atcgatttga tttctgaaac aaaataactg
     8161 tcgccttcaa ataaatcatc ttcgattgcc atggaagtca acacatgtcc aggttgcagg
     8221 gtaaactgtt ctgcaatcgc tgtttggatc gtctgtgcta gaatacagct gatggctata
     8281 cttttgacat acgtagattt tcctgatgca ttggaacccg tgactaatgt attcttctgc
     8341 cagttgactg gattaactac cgcttctttt aacaatggat gatagatatc tttcgctgtc
     8401 acaccgccct cagtaaactc tggtcggcaa gtgatcggca tataggtgcg ataattcaaa
     8461 acggctgctg caacttccaa ttctcccagc aattcccaca gcttgatggc ttcagttgaa
     8521 tgcttttcta atttagtgag aacgaaatga taggagataa atggcagcat gaacatgatg
     8581 ttgacatatt caaaaatcaa ttctgcttca ctcccgctct tcattcgaaa tgaaaaacct
     8641 aatttaggga tgtctcttaa tggtttagtt tgctgtttca gctctttttg aagtggtgtc
     8701 gtcatttttg ctaactgatt ggcggctgaa atcgttgaaa ccaaataact catgctattc
     8761 agctctgttt ctagtgtttg tttcttcact agataataga ccgtattgaa tactaagcca
     8821 ccgattgcta gatagattcc tagtacttgc cctaaaagaa gcaaacctac acccaccagc
     8881 ggaagcaagc caagaaaaat aaataaacct acgcgtccaa tctttttact ttttccatcc
     8941 gctagatatt gttttgcaaa attatgatct ttttttccta aacgagcgaa ttgatattgg
     9001 attctttctc tcagttgagg attttcctga taaaaagcaa ttagtttttc taactgataa
     9061 tcttctccaa aatcaaagct acgtaaacgc tgatacaacg cttctgaacc tacactagaa
     9121 taggtactgt tgatcccttc aaatacttcg aacatatcca agtcatacca agtaagatcg
     9181 tcgatttcac tatcccatga acggaatttt ttctccgtga gccacgcatc cttcaaactt
     9241 tcttctttat caaatctcgg ttgataagga atttttcccc aattactacg aactgtttcc
     9301 tttactttca aacgtgaaaa aatgtctaaa acaactaata aaataacaac tgcaataata
     9361 attaaaacga taaattctct ttggcccata tccctctccg ttccttgatt tgttagtttt
     9421 agtatagcac tgttgcgcta tttgaaaagg gggtacaaaa ataactgtct tcgttaccta
     9481 attcgtatca atcacaccaa aataaaaaca ccttccaaaa aagaagatgt ctttaaaacg
     9541 agatacaatt ttttgtcatt cacttcaggt aaattagagc caattaaacc ctccgcaact
     9601 tgacggagat ggagaagatg cccccgtcta atctagcggt catctctcct cccattcctt
     9661 ccaccaattt ttttgacagg taaagcccga ttccgctaga ttccgtattt gtgcggctgg
     9721 tattttcggt ataaaaacgc tggaagactt tttcaacaga ggtttgcgct cctggtttga
     9781 taggattggc cactgttaat cgaatgaatg aatcctcttt tgaaaatgtc acctttgctt
     9841 gtccatctgc ataacgcaat aaattctgta tgatattttg cagcactcgc agcaacatga
     9901 gcggatctgc taatacagtt gtatttatcg agtgttcagc gaacgctacc tcgatatttt
     9961 tctcagaaaa ttgttcataa aaagagaaaa atacttcttc cacctcttta gaaaaagaaa
    10021 gcgtatggag atccatttct ttttgcttcg tctctattcg agcaagatca taaaactgat
    10081 ccgtcatact gagtaatctt gcaacagatt gtttgatgat cgttaaatac ttctcccgtt
    10141 tctccgtatc ctctacctgt tccagtaatt gtacataacc gttaatagca gtcaaaggag
    10201 ttctgagatc atgagaaagg ccgacgatcg atagtctgat gttttcttcc atttcttgaa
    10261 atgcttgtcc tttttgttca ttttcatcca cccatgtatt cagtgcatct actaggtcca
    10321 ttagattctt ttctcgaaat tcgactgaaa gacgaccacc atattcttgt cgttcagaaa
    10381 gagtggcgat tttcttagtt aatctcttga tttccctttg caggaagaaa ataaaacttc
    10441 ccataaagat aacaacgaga agcaacagcc acttaatcaa atctaaaccc cactccccac
    10501 accgtatgaa tatacacagc atgtgccttt gctagtttcc ctcgaagatt ggaaatatgc
    10561 atattcactg ttttctcact atcaaaatat ggttcttgcc aaactgtttc ataaagattt
    10621 gctcgcgaaa atactttttt cggatgttct aaaaaaagct tcagcagatc gaattctctt
    10681 cctgtcaaat ggacagccac accattcaca tttacatccc tcgactcttt gtccagtaag
    10741 atttccttgt aatagatgcg ttgcgacaga tcttttactt ggacagaagt ctgatgacga
    10801 agttggatgc cgattcgtaa caacagttct tcgatatcaa atggtttcgt cacatagtca
    10861 tccgctcctg ctgccaacaa ggaaacttta tctctttgat cgctttttgc tgaaagaacg
    10921 atgatcggaa cattcgatgt ctgtcggatt tctgctaaca atgcttcacc tgaaagccct
    10981 ggaagcatga gatctagaag gatcaaatcg acaggatatg tgcttaacaa taattttcct
    11041 tccgtacctg aataagcatc aagaagtttg tatctctgtt caagaacctc ctccagtaaa
    11101 gaatgaacag cttcatcgtc ttcgataatc aaaatagttt ccagcaggaa cacctactta
    11161 atgtcagatt tttggaaaat aaacattcct agagctgatg ataccacgat tgttactaga
    11221 ctgaccagaa tcattttcgg tgcttgcgaa taagttgctt gttgcgcaaa gtccgtatac
    11281 atgagccacg tttccgtaaa ccactccgtt agaaattcca agcctttgat ttttgcgaaa
    11341 acaccgggaa cgaaggccgt tcccatgata taaagaaaag aaagaagcaa actgacactg
    11401 gtacttctag tgatgaatgt caaagccact ggaacagata atgtcgctaa tatcagcaga
    11461 agcaatacag gaaatacttt tagaacataa tttccatagt ttttgattcc ctcaacttca
    11521 ccaaatccga aagcaagtgt gtaagcaatc atggctagcc ccgtaatcaa aaccacgcca
    11581 agtgttgtga tcaaaagcgt aacgagaagt ttcgacacat aataagtcat tcgattccgt
    11641 cctaaagata acgtattgcg aatcgtaccg ttttgaaatt cttccccaat ataaaagcac
    11701 gcaaatgctg cgaaagccag aagaatccat gcagccattt tttccaccat taaaatccct
    11761 gttgcaccgt tgatgactgt atccgttgct tggtttaatt cattgccgta ttttgtatgc
    11821 atataccatt cgttccaaga ataaaaaagc ggaagtacga ccatcagtcc tagtgagacc
    11881 cacattttac ggctcttcca tacttttatt ttttcagcag ttaatagatt aaacatgtgg
    11941 attgcctcca gttaattgta agaaataatc ttccagtttt tgcccaacta ggtggatacc
    12001 atccacagga acttttgcct ctaccaatgc ttgattgatg gctgctactt ggtcaattcc
    12061 ttcaaaaagc tggataacac ggctagattg gacaaaataa tccctataac caagagagtc
    12121 gagaaccgtt actgcttgtt ccgtcgccga agtgtcaatt tttataaatt gacgagattc
    12181 ttgtgccaat tcttctttcg ataattcttt gatcaagtgt ccttcatgca aaataccgta
    12241 gtgagtggcg atctgtgaca attcatctag caagtggcta gaaagcaaga cggtgatgcc
    12301 ccgttctttt accaaacgtt ggatgatttc tcgcatttct gccatccctg ctggatccat
    12361 cccattcgtc ggttcatcca aaatcaaaaa ttctggattc gtgatcagtg catttgcaat
    12421 agctaaacgc tggcgcatac ctaaagagaa attctttgct ttctttttcc ctgtattttc
    12481 caatcgcatc attttcagca ggtcttcgat ttctctatcc gacacaccgc cgttcgctgc
    12541 ttggatacgt aggttatctc ttgcagttaa ttctggataa agagcagggg tttcaatcga
    12601 ttgacccata cgtcgtcttg ctgcctgcaa atccgtcgtt ccaaataatt cgatcgaacc
    12661 ttcatcaatc gagatcaaac ccataatagt tctcataaag gtagacttac ccgctccatt
    12721 ctccccaatc aaaccataga tcattccacg tttgatttcg atcgatactt tatctaatgc
    12781 tttggcagca ccgtattttt tggttatacc agttgctttc aagaccgttt catttccaaa
    12841 atccatactt atccttcttt cttattgata agtgaagtat agttgacggg tataaagaaa
    12901 gcagccagaa atgtataaag aaagtgtaaa ttttccaatt gaatttcaag aaaaatcaga
    12961 ctgtctcgca gacgtggttg agttgtctgc caatcaaaga gcgcaccaaa cagtgctaat
    13021 ccaagtggac tggccaccat ggtaatcgtt gttatcgttg acatcagacg tccttgtata
    13081 tgcgttggaa cttctcgctg aagccagacc atataaaaag caaaagaaaa cattaatcct
    13141 taaaaacgga cttatgaaaa ataatacatg ccgtttggga tttacaatta gctaccaaat
    13201 aagaggttat gacaaaagac ttttcacaat cccttcttcc gaataaacgg tgttctcctg
    13261 ccaactcctc ggtcacaagt cacaatattt atcaaacatg agaagcaatt tttgtaaatt
    13321 ccttcttatt tcttgaagct gaccacttct gtcacaacct cagaagcgga taaataatat
    13381 tgtttgatga tactttatct ttttcccgtt tgtttataga tcgaatgata ttatagacca
    13441 agaatcctac ctattaacat agctaaaaac acaaaaaagg cactttccaa attgtggaaa
    13501 gtgccagaaa agttaggatt aaggtaactg cgctcagcat tgcttcgccc agtacgattc
    13561 tcgtaactcg tgaactcgaa cgataatcgc aattaacgtt ttgagaactg tgaagcttta
    13621 cgggctttct taagacctgg ttttttcttt atatcattga atagccttga aaaaccttga
    13681 tataaaaagg ttttagtaaa aatgatttta tcaaatttga ataactttga aatatttgtg
    13741 ttctaaaatg tgttctaatt taaaggtagt atatttcgct tttttgaatt aaattttaga
    13801 aacaaataat ttatcagcat ataaatactg ataaaagttt tatagacaaa gatgttcata
    13861 gcgaacatct tttttattta ttttgagaag aaacagagca tgaaatgatg tcttatttcc
    13921 atccatatag tctatttcaa acagaagaga acaattaaaa agaaaggaga tttcttgtta
    13981 tgataagtaa caaaaaacgt tggttaaaag ttgctttact ggtagctttt tgtcaaattg
    14041 ctttcttggc ttcaatgaca acacaagcgg aagaaatagt tcaagaaaca actgtttctc
    14101 aagtttctcc tacttcaaat gaagtagaag tgcaatctac tttaccaaaa gaggccgtta
    14161 aagaagagac accatcagaa gttgttccag agaccgaagc accttcaacg aacgaaaaaa
    14221 ctgtactcga tgaaactatc gggaaaagcc aacaaccagg agatagtcca gttgtggcag
    14281 aagaaacccc agtagaagca catgaagctc aagcaattcc tgaaaaggac aaatcattag
    14341 aagaaagtgg ttctactgaa ataaacgaag gaacagaacc ggttcaatta gaagattcaa
    14401 aggaggtaac gactccgatt gaatcaacga aagaagaaat ggattctaat acttctcttt
    14461 cttctgagaa agaagagaaa acagttcttt tgacagaacc tcgtgaacct gaattgaaaa
    14521 aagtcgagga atttgttgat acaaaaaaag aagctgtttc tcctacctct tcaaaagaag
    14581 atacattgat tgtacaacaa accgctcgaa gtgcacgggt gcgacgtgca accaatacag
    14641 cagttttggg tggtcggaaa attgattggg gcgaaaatat tggtgttatt tctagtgcca
    14701 ataatcccaa tttcacttta aattacatgg ttcgttttga gattaatgga caagtagcat
    14761 tttgtattga acctggtgtc gcagccgcag aaggtcaagt ttatacagct actgctttag
    14821 aaaactattt gaaagattta aatatacgaa aaaaaacaag cttgatctct tatttaggat
    14881 atgtcacaag caaagacaaa tccgatgaac aatacattgc ggcacaattt atgatttggg
    14941 aagttttggg cacaaaaatt ccaattaaaa atatccgtat tgactatgat cgtcgaaaag
    15001 cagaaattca acgtttgctt aataattttg atgtaagagc tacttttcat aatcaaacac
    15061 atacggtaaa aattggcgaa acgttaaaag taaaaagtgg caatgacttt ctacaacgtg
    15121 taaaagacat tcaaaagcca aaaggtgtta atgtatctat tcatgataat gaagtatgga
    15181 ttacaatcac aaaagagtca ccagaaaagg caaaaattca tttcaatcaa attgaagcat
    15241 taggcgtgcc attagcgtat cgtcgtccag gtgctcaaac aattggggtg ctgaatcctt
    15301 ttgtacctgg tgactctgtt gtcaatttga ccattttgaa acaaggaaac attcgtgtct
    15361 ttaaagaaga tgcagaaacc ggtgtgaaag cacaaggtgc agcttcttta gaaaatgcag
    15421 tgtatggctt gtatcaagca aatgggaaga aaatcaaaga aattacattg aaaaatatca
    15481 atggcaaagt acaggcagaa atcaaagatc ttgatcctgg taaatatgtt ttaaaagaga
    15541 tcaaagcacc aaaaggttat gtactttctg atgaagcaat tgaaatcgtc gttgaacctg
    15601 gaaaaactgt caatacgatc gcaaaagatc aagtgatcaa aggaaccatt gatttagtca
    15661 aagtcgcaaa taaagattta gtcgattcaa ccaatccaga caacaaaccc aaattagctg
    15721 gtattgaaat ctctttgacg tcaaaaacaa ctggaaaagt ggtgaaaaca gttgtgacag
    15781 ataatgatgg ttacgctact tttggtaaaa ataccgttgt ctttgatacg tacattcttt
    15841 ctgaaaccaa aggaaaagaa ggatataaac tatttgaacc attcgaagtc acaatttctg
    15901 aaaatggaca aaccttccat tatgttttag aagacaaagt aatcgaacaa cgtcttaaag
    15961 tggtgaaagt ggatcaagaa acagggaaag tcattgctct agctggtacc caattcaaaa
    16021 tttgggataa atacgcaaac aatggtaaag gtggctatgt atcgatggca ttaccgaatg
    16081 atacagaggt ttcagacata tttacaacaa acgagaaagg gtactttgtc acaacggata
    16141 cattgaaata cggaaaaaat cgttacgagt tgcgagaaat caaagcacct gaaaaatatg
    16201 ttctaaataa aacgccttat gtcttttcag taacagacga caccaatacg attcaattaa
    16261 tctacttcgc taaccgttta gcgaaagcca acgtagaggt tttcaaatat gatactagta
    16321 cgaattttga tcaaaaaacg gcgattgctg gtgttgaatt tgacctattc aaaaaagatg
    16381 aaacaggcac aacctttgtt ggtacattca aaacagacaa aaaaggtaaa atcaatgtga
    16441 aagacttatt ggttggcgat tacttcttta aagaagtgaa accattagca gggtatctgg
    16501 ccttagataa agacgtacca tttactgtaa cggttgataa agacggtaca gtgattgcac
    16561 tagaagtgga aaacaaacgg attccaccta aaattggtac tacagctatc ggtaagtata
    16621 caaaccaaca aaaagtgacc ccattgaaaa aagtaacgat tacagacact gtaaaataca
    16681 gtaatctaat catcggaaaa acttataccg tcaatggtgt tttgatggat aaggaagaat
    16741 ctatcacagc aggaaaagac gtaccgttgt tagtaaatgg taaacaaatt accgcaagta
    16801 aagtgtttgt cgcaaaaaat cctaacggaa tgatttccct tgactttgtc tttgatgcta
    16861 gtgcattagc tggaaaagag atagtcgtat ttgaaaatgt ctatcgtgac ggacgcttag
    16921 ttgcgtctca tacagatatt cacgataaag gacaaacggt tcgtattgtg aaccctatga
    16981 ttcatacaaa agcaacaggt caaaacggcg aaaaacaatt acaagcttta ccaaatcaaa
    17041 aagtgaaaga attggtacag ttgaacgact taattgttgg tcaaacgtac aaagtgctcg
    17101 tacaaggtta tattacacct gatggtgtga aattagacgg tacaagtgct gaaaaaattt
    17161 tcaaagcaga taagacaact ttagagttct tatttgaatt tttggtagat ggacgtaagc
    17221 tagcaggcaa aggattatct tttgcagaat ggttagaagc ccaaagtgaa ccaaaagatg
    17281 aaaacgatta tgaagttcga tttgaacaaa tcgcaaacca caacgtagac ttaaaatcag
    17341 aagatcaaac ggtttatttt gtagaagttc caaaagtacc ggaaccaaaa attcctgaaa
    17401 aatcacaacc ttttaaagag gtgaaacaag cttcagtagt accaatgttg ccaaaaacag
    17461 gatcagaaga tactgtatta tggatgatca ttggtgtgct atttgtttta gcagcaggtc
    17521 ttacatttta cgagttacgc aaagtcaaag actaagtagg acaaacaagc ggttagatgc
    17581 ctaatcgctt gtttggtttt aaaaaagtta ttgcataagt aacctttgaa aagataagaa
    17641 atgatctttg ttttatgaag aaaaaagaaa ggaaaaagag aatgaagaag ctatttttcg
    17701 catgtttatt tagttccgta gttcttgctg gtggaataaa cgtttttgca gatgaaatag
    17761 gtattacacc agttacgcca cctgtgatag aagaaacaac acctgttgtt ccaattccag
    17821 atatttctaa aatagatgag tctgttccgg atcaaccaac tcctgttgat ccggatatat
    17881 cttcagaagt aactgaacca acaacacctg ttgttcctga tataacggaa aattcagaaa
    17941 atgaaacacc agttgtatcg ccttctactg gagaaacaac gaatgataaa gatactacac
    18001 caactaccag tgagtcttat tcgggaacta gttcggtaga taatgattca aaaatagacg
    18061 attttagttc ttcatctact acaatagata caccaaaaga aacagaaaag aaaaatgatt
    18121 taggagctcc caaaaattta acacctgaaa atcatataaa gactgtatcg gaaaaggaaa
    18181 tgactgtttc agtcagttca gatggaaaaa ttgtacaagg aacgggtacc gaaaaatcaa
    18241 ttcctattgt gacaagtaat ttagaagaaa ttaatcatgt cccaacatct gaaactcctt
    18301 tacaagtagc gactggtcaa gcgattgttg gggtacaaga tggtgtacca cttgtacaag
    18361 ataatcaagg gaatttggta gaagatttat ccattcctgt aaaaaaacta cctagcggca
    18421 atattgaaat caaaacaatc gatgggaaaa caaaagtatt gccaaaaaca ggtgaagaac
    18481 ttcaaattag tttaactatc cttggttctc ttttaacagc agtaaccggg ggcatttttt
    18541 atggtaaaaa acaatggcgt catttgcgtc atatgtttaa gaaaactatt tagtaaggag
    18601 agagaataaa aatgggttta tcttatgttg caattagtca ggaagaagta ttgggaaagt
    18661 gttttaatca aaaacacgtt ggctcgacag gtgaacgaga tcgtcgagga aatatgaaga
    18721 aagtagagtg ggagattttt cagatgaact ctgaaaagat gggagaaatt gaagtgcgtg
    18781 tagctgtcgg tggtgcgcct aaagccatgc gatacaatgc agaagtgtta ctggcaaatc
    18841 ctagaatttc tcgtcgagca gatgtacgaa atattggaaa tgacggtgaa acaaacaaaa
    18901 tagcacgtaa taggttttat ttgttagtcg atcgtgtaga agtaaaagga ggaaataact
    18961 aatgacacta gaatttgatc aaggaattgt cccagggttg gtaaaagaaa cactaggaga
    19021 aatctatttt gtcaaggcac aacgtccacg tatgctgtat gaagataatg aaaacacagg
    19081 agaaattaaa gagcgtccag ttgaattttc aagtgctacc caagaaacaa tgttcgtggt
    19141 gaattttcca ccaaaaacaa atttggatgg attaagtttt ggcgatgtgg tggaattaga
    19201 agaggttgaa tttcgatttt ggatttctat tgatcgcgaa tcaatgagta accgagcaga
    19261 gaatgatctg aaagtatctg cgcttcgtta taaaaaaacg ggaaaaaacg tttttcaaac
    19321 gtcttcctct actcgtgcaa catatgtaaa agaaaaacaa gactcaaata aagaaagtgc
    19381 aaagaaagag taatggtttc cattgctctt tcttatttta aagctagaag aagtagctga
    19441 tgtggagaaa attgaattgg ctggctatat tattgggata aatttttgaa tttttaagaa
    19501 tttagatagt ggttagatac gattgattct ttgtgtattt tgtattattt aagtattgtg
    19561 tggtatttac attaagttaa tattgatgtt gttagtgtta atgaaggttt tcttttatcc
    19621 aatacattag tcactctatg atgcgaggag atgagaataa gtgtttcaat ataaaggtat
    19681 tcgtattcga aatcgacata aatatgcttc agaacgattt attttttatc ttatgttgcc
    19741 ggttctgttg ctaatgagtg gatttttttc tctcttttat acaacgatca catgggaatg
    19801 gtgctgtatt tattggataa tcgcatttgg tttagctggt ctatttgcgt atatgtgttt
    19861 gtatttttac ttttataatg tttatcagcg attggttcat aaaggaaaat tagcaaagat
    19921 gatacatagt cgtcgattga ttcttgaaag agtattgaaa aatggacggc ggaaagtggt
    19981 gtattatcca aaaatttttt atcactataa aaatggctta attcatgttc gattgccatt
    20041 agatttaggt ccctttcaag atcggtttaa tgagatggct gggcaactag aagaagcctt
    20101 tttttgtgat ttagtagaag agcaacgaga gaaaaactat atttgttata cctttttata
    20161 cgatgtggaa aaaaatcgta ttgggattcg agatataacc gtaaaagatg gcataatcac
    20221 cttaatgaag aatgttcaat gggcatttaa taaagcacca catgccataa ttataggaag
    20281 tactggcggt gggaaaacgt attttataat gtctttgatc tatgcattat taccgtatgg
    20341 tacgtttgat atttgtgatc caaaacgtgc agatttggct agtttagaaa aaataccaat
    20401 ttttaaagat caagtttttt atggtggtgg tattttatct tgtttaataa atctagctaa
    20461 agaaactgct aatcgatacg attttttaag agaacagccc gagtatgaat tagggtacga
    20521 ttatacttat tatcatctaa ctcctcattt tttggtgatt gatgaatggg cagcatatta
    20581 tgcatcgtta gatactaaag aaaaaaagaa ggcaatggga tgtttgaacc aaattgcttt
    20641 aatgggtcgt caatgtggcg tgtttttaat tttaggtacc caacgtccag atacaaagta
    20701 tttggacggc gcgatacgag ataattttgg tttacgtgta acactgggaa gagtatccaa
    20761 agctgggtat aagatgatct ttgaacatac agtaaaatca ttatttaata agccgattaa
    20821 aggtcgtggt tatattgatt cgggaaccga tcaagtacga gaattttatg cgccatttat
    20881 cgatataaca agatttaact tttttgcgga atttaaacaa ctatctgatc aattaatggc
    20941 tgaaaaaatc aaggagacga aagagggaaa aaatgtatga agaaatccca gctacatgtg
    21001 gaactcgatt agaaataaaa gatttaatga ttgactcttt tttggcaatt gttttcagtg
    21061 agaagagatt aagcttcttt atcaataaaa aagtagagaa tcgttggaga ttagaacgta
    21121 tgtatccaac gagttaactg attggttaca gttgccttat gacaattatc gaaggcgaat
    21181 atgacgggtt tttacttcaa ataaaagtgc tcgatcctat agtgaccaaa ttgtcgaaaa
    21241 agcagcggaa cagcctgttt tttatggacg attcaaagga ctttcctgtt cttatgttaa
    21301 aagtaaagac cggtctcgac tgtacctaca agtagaggat attaacttgt cctttgcgtt
    21361 gtcagaaaat taaatttgtc ctcattagaa tacggaagat gctgtgcgtg tgctccacaa
    21421 gcaaacaagc ctcagggcgg aaggacgcgg aagcgtccta gcgttagcga aagccctgag
    21481 gtgcagggtg cgttgtgggg cgtgcgcgta gcgcacgcct aaaaccccca tattctaaca
    21541 ggggggtaga atttatattc ggtggcaaag atgggtgatt gaaacattga tataacacta
    21601 ttttgcttca aaaattttgt gatatttttc ttcgctttgt aacataaaat atcacaaaag
    21661 aaaggaaaga tatatttgga tgggacacaa ttaaagcaaa tgcggttaag tcggaatttg
    21721 acgcagaaat tagtggcaga tacattagga tggccacaat cttttttatc aatgatagaa
    21781 aatgaccaac gaagttgctc gaaagaacaa gaagaagcac tttgtgaatt ttatgcgaat
    21841 ttttcaagtt ttacgagttt agaaattaaa tttgattatg tccgtgtacg agttccgaca
    21901 aatgatacga aaacagttgt ggaagaaatt ttgggaatgg attttaactc ttttttttat
    21961 gcagaaaccc gcttatatgg ttatattgaa atgtatcaaa aaggcatgat ccgtgtatta
    22021 aattctaaaa aaggtgacga taaaggagtg ttgatcgaat tatctggtca aggctgtcgg
    22081 aattatgaaa gtgtactgga tgaacgaaat gatagttggt atcaattctt tacacgatgt
    22141 gtctctttca atggtgtgcc tcgaagaatt gatattgcgg tagatgattt tgaagaacta
    22201 ttttctttac atgaatttgc acaaaaacta aatcgtggcg aatttgattc aaaatttcga
    22261 acctggcggt ttcaagaaga aaaacagttg ttagataatc taagtagtgg cttatctgtt
    22321 tatttaggta gttcacaaag tttgatgtat ttttgttttt atcagaagaa ttatgaaatt
    22381 gcaaaaaagc agaaacttcc gttggaagag gtcgaggtaa aaaatcgtta tgaaattcgc
    22441 ttacgtgatg caaaagcggt cagttttatt gaacaatact taaataaagt agatttttca
    22501 gatttaacga tggggatttt agccaatcag ttaataatgt ttgagggaga gaaaaacgca
    22561 aatgtgtgga aaccatgggg aaaattgatt cagaacgttg gaaaagtcaa tttgacaata
    22621 acaccagaaa agccaaatta taatcggaaa ttgatgtatt taaagacaca acctgctcgt
    22681 acaatgaaga ttgtaaaaac tgtggatgaa attttaggat tgaatcgatt tggagaaatt
    22741 ttagaagaaa cagaattacg ggaacaagat gaaaaaattg tagaagatga ggtgcgtgct
    22801 gtatatgaat atatacaaga aaatccagac gcgattcatt atttgctata aagcttgcga
    22861 taagatgggg gaaacatgat taaaacagaa gaccaatgat ggggagagta tatgattgct
    22921 tgttaacaat taataaaaga aacgatccta tgtgttgtaa atgatctaca gttatttaaa
    22981 gaatatttag attatcaaca atcaatcaaa atgttacatg tagaaatagg ggaagtatta
    23041 agctaccgta ttagtgattc gttaaaatat tcgttgacaa agatttattt atatgcaaca
    23101 caagtatgtg gatatacgaa aaagcagtaa aaacaatgat gaaatatgac gatttgccaa
    23161 gcaagatatc ctagatttac aacaagtatt ggaagaaaaa ctgtatctat atatgaaaaa
    23221 tagaggggaa aagaaatgat tcgattttta ttttctgtag gttggcattt tttgaaatta
    23281 ctgtttcttt tagtttgtat tgtctgtagt attatttggt tattaacact ttaaagaaag
    23341 gaatatgttg gaaaaatggg acagtatttt cttaccggtg tttggtatgt attgaaatta
    23401 gtgattattt taggtattgc ctatatttgt tttagaattt tattatcggg agaaggaatg
    23461 ccaacgccaa aagattttat cataagcgtg ttgaattttg tggcggatca aatacaagtg
    23521 ataattcatt gggcgcagca acgttttgga taaaaaaata tatttaaaaa atttaatgtt
    23581 tagaagtggg ctatgtgata gaaaatttcg agtataagtt atctagattg ccaaagaaaa
    23641 agggagctac acaaaaagag ctggctaata aattaggtgt caatgtacaa agcgtttcta
    23701 atgttgagga agataagagc taactacatt tagtaattta gaaaaaaaca gtttctttct
    23761 ttgatgcttc atcgattgaa ttatttggta cgaataggga tcgaataatt gcagatgttc
    23821 cattaatatt agaagatatg gataatttgt ttgaaaaaat tcgaatgatc tttatgactg
    23881 aaaaagtaat caaacaatta aaaaaaagtt tctgtttttg atggaaaaaa actatcaaaa
    23941 agaatcaatt attgatcttt ttgatagcct ttatttcgtt gatggaatcc atttattagc
    24001 tgtttcaaca atcgttgttg taaaaaagaa ttgttttgga tgcgtaattc tggatagcct
    24061 tcaatggctt gattttgctt ttcaaatgct tgttttgcat gatatgcttc acgatctaaa
    24121 ataaatagat aatctgtttg aaatgcttca aatgtacaga ttttttgagt ttttaacaaa
    24181 tgaagatagg tattgacaaa gtgttctaca cacataaagt tttttgtgtg tgaggcaaaa
    24241 agatattctt tagctaaaaa gttagtagta agtgtatcag ataaataaaa gtaatctggt
    24301 gcgcgatcat acatttcgac aaaatagtca taaagtaaag caattttttg tttctctgct
    24361 agaagatact ctatttcttt ggaataaaac tcatcaattt gatgagtgtg tttaataact
    24421 tgaacaaata atttcatata gtgttgaatg aattctgttc gttcgattgg ttcaccaaag
    24481 ggttgaagta tggccatcat tctttctcct ttcactcaag ggaaaaatcc cttgagaatg
    24541 aatacatgta tttagtaaaa aaatattttg actgcttcga tggttttttt tccagtttgt
    24601 tttgcgataa gtttaggtaa aatctgttgt aaccattgtt ggtcagatac ctgatcgaag
    24661 aagtagccga ttgttgttaa aatgagtgta tctgttgtat gttcaactaa acgtacagat
    24721 tgaaaaggac ttaacccagc aacatagttc aaaatatttt catctgtttg ttcaaaagat
    24781 cctaaatagt tattggtatt ccgatcgtaa gaatcaacga taggaatagc atttgttctt
    24841 agcattgcaa tctctccttg attagaaata ctcagttatc attttttatt gatagattta
    24901 gaagcaagaa ctcttaattt atgcgttgtt ggtaaattgt cagaccgttc ttcacctaag
    24961 atttcatata gagttattgg tttcattgtt cttgctcctt ttatttttgt tatttttaac
    25021 attgactaag agggaaattc cctcttagta ataactgctt tcataaaccg gttgattatc
    25081 atagcggtaa aatgtatccc aactgcgatt accgtataat tcattgggat ataaggctgg
    25141 attaattcgt tttctaacaa tgctgtcttt ggttcgtaca agcggataat cgataaattc
    25201 atttggtatt ggcatagttt ctccactcat agaagtatag gtttttacag tttgcttttt
    25261 gatgggtcga aaccatgctg ttttttcggt taatttaata atttgccaaa aattaacgtt
    25321 ggtttgttca tagccccaac tactataaaa aatgtctcct actttgtatg gtgtatttgc
    25381 tttcatgatc tattcctcca ataatgtttg atttgcctta tttgatactt agccgaatca
    25441 accaaccatt ttcggcagca agttgtctcc gaggtgggcg accttttggg agatttcaat
    25501 ttctttttgt ttctattcac ctcacttttt gtttttggtt ggtcattttc gtatagctaa
    25561 gcgttcgtcg gcgtgctttg ggttcacaac gtgtggtaag agaaaacaag ggaatgctgt
    25621 aggaaaaagt tttttatgaa atgatcaaaa aagtttttcc gccccttgtt ttgtcttacc
    25681 atccgttgta aaagcaccgt cgccgatgat cgcttagatt gggtatatga aagtgaccaa
    25741 caaaaaaagt atgggaataa aaagaaatcg cattgaaatt tctcaaaagg tcaagatggt
    25801 tttaataaaa actttctttt aatacatttg ttattttgat aatgtggtga tataaaatag
    25861 atcaaatagt taaatgaggt gtttatatga agaaaaaagt tattccttat tgtccaaaac
    25921 ctaaaaatgc ttttgaaaga ggtttgttta aatattttga aggcaaaaaa ggacaagacc
    25981 ataaagagga gaatcaaaaa aagaagtcta agaaaaaaag tgaggagttt ttttagaaag
    26041 aataaaactg gaacgtaaag atttacaagt aatcagtaac ccgtaccttt tatttttaaa
    26101 gaggtcgttt ttctgtaaat cgttgtaaag aatgactaag ttgtattatt ttttcacaaa
    26161 gatttatatt ataatgtttc tttttaataa aaagcttgtt tttaagctaa ttattatatg
    26221 tgttttattg aactaactct tgacagatca tcaaaaagaa tttatagttg ttaccgtagt
    26281 agctactaaa aataaagtga ggattaatca atggaaacga ttattcaaat catgaaaaaa
    26341 tatgattttt cagagatgga aacaagagtg tataccacac tattagaaaa aggtaatctt
    26401 acaggatatg aagtaagtaa aatctcaggt gtgccacgct cgaaagtata taacattttg
    26461 gaaaaactat taaaaaagaa tttgattgtc gttaataaat cagaacctaa actttatcat
    26521 gccatctcgg caaatgagtt tttagagaaa ttagaaaaaa gtgttaaaaa tgatctttct
    26581 tttcttacca agaatctagg gatgatcaaa gaaaaagatg aagaagaaat gctgtggaaa
    26641 gttgatggca tcgagtatgt tctggataaa gtagagcatt tggtgaaaaa cgcaaaagaa
    26701 agtttgctca ttcaagtttg gcatgagaac ctcacggatt cactgctaaa gacattgcag
    26761 caagctgaaa aaagagttga taagtttgta ctaattttat tcagttctac ccatgaatat
    26821 gatcttccct tagaaaaata ttatattcat ggctttgaaa cagacaaact tgctgacttt
    26881 ggagcaagat ggataaacat cgtcgccgat gagcaagaag tcgtgtttgg caccattaac
    26941 gaagaactgc aatcaaccga tgtcacttgg acaaaaaacc acgccatggt caatctagca
    27001 aaagaatatg tcaaacatga tgcatataca ctaaaagtga ttgcagaatc ttctgaagaa
    27061 cttaaagcaa aatatggtga ggactttgag ggaatcagaa agatttacta gtaggaggaa
    27121 atttttagga ttcggaatat tttgctgaca ctaattgtcg caatcaatct atattttatt
    27181 atttactttc taagagacct tttcaaaaac aaacaaaatt tcaaagaaga acctggggat
    27241 atgcgtctgt tgcccttcac gtcatttatc atcttctttc tttcaacatt tggcgtttcc
    27301 gactttgcta tcggaaccgt tctttatcca aagttgaaat gggtcagcat gaaaaaacta
    27361 cccggaacgt tgaataccca atgtgtcgta ccggtagccg tcatggcatt gtcttatatc
    27421 actgccatca acgtagggat caagacacta gctgtttgta ttatctgtca aattatcggt
    27481 gcatatttag gaccaagatt tgtcgtgaag ttaccagaga aaacgatcaa agtgtttgtc
    27541 ggcataggat tggtcattgc tgcattattg atttttgcag gacaaatgaa ctggattcca
    27601 tcaaacggaa cagccagtga attgtatggc ggaaaattaa tcttggcagg atttttactc
    27661 tttgtctatg gcgcattgaa caatatcggc attggttctt atgcgttaac gatggtaact
    27721 gtctacttgc taggcttgaa tccagtggca gcctttccta tcatgatggg tgcttgtaca
    27781 ttctcagtac cgatcggtag tgttcaattc attaaattta atgaatacag cagaaaaatc
    27841 acacttttca cttcaacctt cggtgtattg ggggtattgg tcgctgtatt cctagtcaaa
    27901 tctttagata cctatgtctt gaaatggatc gtaattttgg tcttactata cagtgcatat
    27961 acaatgttgg gcagtcaatt aaaaaaagtt tcagcagcta attaaggagc gattcaaatg
    28021 ttatttctta aaaaagaaga tattatcaaa agtttctcaa tgagagaagc catcgatgca
    28081 gacaaaaaag cattgtcatt gtattcagca ggaaaggcaa gtgtgcctct tcgcaccaat
    28141 atcgatgtgc caaaaagcaa cggacaaagc ttatatatgc cagcttatgt agaaggtggc
    28201 gaaggtgcgt taggtgtcaa aatcgtttcc gtgtatcctg aaaacatcaa gaaaaactta
    28261 ccaagtgtac cagcaacgat gatcgtcctt gatccagaaa cgggtatggt atccgcttgt
    28321 ttggatggta cctatttgac acaattaaga accggcgctg ttcaaggtgc agcaacagaa
    28381 cttcttgcaa gagaagatgc taaaatcggc gcactgatcg gaaccggcgg acaagcacag
    28441 tcgcaattag aagcgatgtt gaccgttcgt aaattagaag aagttcggat ttttgatatt
    28501 gattttgacc gtgccagccg atttgctgaa gaaatgatgc aacagttttc tgtaacgatg
    28561 cgcccaacaa aaaccagtca agaatgcgtt gaaggagcag atatcattac aagtgtgacc
    28621 acttccaaac gtgcgacatt ctctgctgag tgggtcaaaa aaggtgcgca catcaacgga
    28681 gtcggtgctt acacaccgga aatgtgtgaa attccacgag aaataatcaa agccgcagat
    28741 gttgttattt ttgataccat ggacggtgtt ctaaaagaag ctggggactt catttcaccg
    28801 ctgcaagatg ggtatattca aagagacagt tatcatgggg aactaggcca actgatcaat
    28861 gaagaattga tcggtcggac aagtggagaa caaatcacaa tcttcaaaac agttggttcg
    28921 gctgtattag atgtggtagt tgcgacagaa atcgttaata aagctaaaga aaataatttg
    28981 ggaaaattga tttattaaat aaaaagaccg gaagtaaacc tttaattagt acatttataa
    29041 aaagagaaca aaaaagactg taatcaaaat gcgattacag cctttttttg ttttatttgg
    29101 gaggagatag atcgtgttta tgactgtatc agatcattta aaggtacttg gttttatccc
    29161 tttatctagt agagggaagt atcaattgta tttattagat ttaccaccct agcttttcac
    29221 tggtgattga aggaacatac gataaaaaaa gtggtaagta tttttatgat ttctattcta
    29281 tgaaacttga tcctaaaaca aagaaaagta cgcagcaccg gatttattgt acgcatgttc
    29341 ctgtaaaagt aatgtatcgt cgtgtgagaa attatttata cttcttacgt aaaaaacagc
    29401 aatcaaggaa cggaaaaaat gcataatgag gaatatcaag tgaaattttt atcagttttg
    29461 ctctgtggta ttgtgtatcg tcattagttc aatgagtatt aacagggagg aaaaagcaaa
    29521 tgaatttttt tcaaaatgtg tttactgatt ttgttgggga ttttaagtat ttgattatgt
    29581 tggcgatgct tggtggtgcg gcatatattg cctttgaacg gaaagtaagc aaagccattc
    29641 cattgattct tgtgctagca gtagctgttt ggttagttgg agatacgtct ggcgtattca
    29701 attggatttt ggatcgtatg cggtcatggg ggcgataaaa tgaacaaaat acgcaattgg
    29761 acaaagattt ggtatataga gcgagtgttt tataaattgt gggatggggt cgcgttacct
    29821 cgtcccgtaa cacaaacatt tgcgatatgg tttgggattg gcttttttgg ggggattttt
    29881 cgaatttttc catttgttat ggaacatacg attactcgac aatttttttg tccgtggtta
    29941 tttgcttttt taatgacacg aaaacaattt caaggaagga agccttataa ttatttggct
    30001 tcttttcttt tatttcagtg tcgttcaaaa gaatcctttc atggccaatg ggtaaagcca
    30061 atagaaaaaa agaaatcaaa cgaggaattc gttgtgtagt acgttatgga ggtgaaaagg
    30121 atgcgactac caaaactagt gcctaagatt cctttttatt atttagaaga caatttagtt
    30181 tttggagaag aagatcgtgt gtacgcttat tatgaattcc caatgtattc atatagcttc
    30241 gtgggagaag atcaggcgta tcaactccaa caagcaatca tgcgtctgat tcgtcaaagt
    30301 tattcttcgc atgttcggtt ctttttttgt acaaacgaag aaaaaattga aaaaacgatt
    30361 aaagcctcaa aagaattggt gcgttctgaa ggggaattaa aaacgattgc ctaccaacat
    30421 ttagaaggtg ttcagcacat tttagaagag ctacatggaa aatatattca aaatgtttgt
    30481 tggtatgttg ggattgagct ggtattagaa gaaagtgctc ccaaagaagc tcattggatg
    30541 aaagaatttc gtattgcgac aaaagatttt tttcaccgat ccaatgcccg tttgtttgga
    30601 gactttgaaa ccatcccaaa tgaaaaaatt caacgatata agcgattgga gaatttattg
    30661 tataaccgga ttagtaaata ctttcggtta cgaaaaacaa caccagaaga catggtgttt
    30721 ttagtggaac atttagaagg aaaaaaagaa caatccttag atgaagtgat gtatcgaccc
    30781 aatagcttta aagaagaaac gaatacgtat acgtatcact atgatgtatt gaatttggcc
    30841 agtgcaggaa tcacagaaaa gaaaaattat ctaattttag aacaagggac agaaaaagaa
    30901 tatatcacgt atttatctct ttcacgtatg acccgtgatt tggtatttcc atttcaatca
    30961 gagatttatt attttttaca atcggaattg gattttccaa tcgattcttg gttagatgtg
    31021 aatcttttag acaatcgttt ggccttggcg aaagtacgtg gcaaaaagag tgatttatct
    31081 gatatttatg aaagtggtat gtcggttgga aaagatgtag gaaacaatgt acgcgaagcg
    31141 taccaatcag ccaatgagtt ggaacaaaaa ttagaagata cgcgagaaga tttgtatgta
    31201 ttatcactaa acgttcgcgt gtcagggaag acctatgatg aaatgttaaa acgtgtgagt
    31261 gatgttcgca atttttttga tgtctattct atgtatttag aagtaccgtg caatgtacag
    31321 ctgtttttac attttggaag ttatccagca ggaaggcgag tgattgagga ttatcgtcaa
    31381 tatgtaggcg cagaatttat tgctagtctt ggcattggtg cgacacaaca actaggagat
    31441 ggctgtggta ttccgattgg atttaatgca gaaacaggaa tcccagtgtt tattcaacca
    31501 tggttagcgg cacaaggaat aaaaggaacg gttaccaata ctcttgccaa agcattaatt
    31561 ggttcgctag gtggtgggaa atccttaact gaaaatctct tgttattttg gtcggtgttg
    31621 tttggcgcct tggctttttg tatcgatcca aaatcggaac gttcaaattg gtccgaagat
    31681 ttgccatatt ttgcccccta cttaaaaaca gttaatatta ccagtgagaa agaaaatatt
    31741 ggtttacttg atccatttca attgatgaga agaaaaaaag atcaagaaag tttagccttg
    31801 gatgtgctca cctatatcac gggtgtaagt attcgggatg aagaccggtt tcctttgtta
    31861 caacgggcag taaaaactgt ttctgatttt gagggaacaa aaggattact tctagtgatt
    31921 caggaattat atgcacaaga agcagcgatc gcacaacgcc tggcaaagca tattgaaagc
    31981 ttcaaagaac tatctattgc aggcctatta tttggagatg gcacgaatca acaagggtta
    32041 aatttggatg ccaaatgtaa tctcgcactg gttcaggatt taacgttacc agatagtgaa
    32101 actgcaccag aggactataa ctcttctgaa attttaagta tagcaattat gatgagttta
    32161 gctacatatg cgctagattt tattaaacaa aatcgagata tttataaagc ggttggctta
    32221 gatgaaagtt gggcgtggtt gaatgttgcg caaggaaaaa ttttaggaaa taaattggtt
    32281 cgtgaaggaa gatctcgcaa tgcagggatt gatttttcaa cacaaaacac agatgattta
    32341 ggcgatgaaa agatgagaag taaaattggg ttgaaattca tttttcgttc gaatgatcgc
    32401 gaagaaatta ccaaagcatt aaacttttgt ggaatctctg tcacagaaga aaatatccag
    32461 cgtgtaaaaa atctagaaaa tggagaatgt ctgttttgtg acatccatgg taatattggc
    32521 attttgtatg tgtattattg gtttgaagat ttattccatg catttgatac acgaccgcca
    32581 atggaaaaaa actacgaaga ggaagaatat aaagacgaga tattagaaaa actggttgaa
    32641 gaaattgagg tgaattagat gaaacagtgg tattcaaagg tttcattttc aattactcgc
    32701 cgaaaactgg ttttcataag tctagtcata atgatgggtg tttttttctt tcacccaata
    32761 ttcgtagaag cagctggttg gataaaagaa accctaggcg gcaaaaatgc cgtatatgat
    32821 tttgaaacat atcctttaga taattataat ttagattttt ttgtggatac gtcgtggaat
    32881 tggttgccgt ggaaatgggg tgaaggaatt tctaatacag ttatttatgg gatatttgcc
    32941 gttaccaata ttttatggat gttaaatgtc tatctgtgtt actttttagg gtttcttgtc
    33001 caagaagcct ttgaattaga ttttctttca gagttgattg gttcgttaag tcattcaatt
    33061 cagaatattg ctggaatgaa ccgtgcaggg acattgagtc atggattagt ccctttcttt
    33121 ggtggattgt taattgcttt agcaggttgt tcagtagttt atcaagggat agtgaaacaa
    33181 caccctacgc aagcgattca acaagcagtg acatttttat ttacgtttat ctgttgcggc
    33241 attttcttta tgaatacgca cacatattta gagaaagcca atgaggtaca aaaagaaatc
    33301 aatagtgaaa tgttaaccat tgccaaagat ctgttacctg aacaaaaagg aaaaccagat
    33361 gcaattaaag ccattcgtga aaatctattt gccattcaaa tcaagcaacc ctggttagta
    33421 ttgcaatttg gggatagcga tataaaaaca gttggtgaag aacgtgtaat caatttatta
    33481 aaagaatctc cttatgatga aggagagcag cgaaataagc ttgttgaaga agaagtaacc
    33541 aaacaaaaaa atggcaatct ttcggtacaa aaagtgtatt tgcgttttgg tatggtgttt
    33601 ttaacgctca ttgtcaacat tgtcattagt atgggtgtat tggcattagt cgtcatctta
    33661 attgcgtcgc aagtattgtt cttgttattt ataggatttt tacctgtagc gatgattttc
    33721 tcgttgtttc caaattcagg atcgttatta ggtaatgcct tacagaaagt ctttcaatta
    33781 ttgtttacaa aaatgggcat tttactcatt ttaaccatta ctttttctat tagtcacgag
    33841 ttaaaccact tatccaagaa caaaggaatg atttggacca gtttcttaca ggtgacgtta
    33901 tggttttcag tctcgacacg tgtcaatgaa ttattaggtt ggatgaaaat tggtggcgca
    33961 caaacacgag ctggggatcg aacgggacgt tttttacgtg gcatgatgtt aggtacaatg
    34021 ggtcaaaaaa tgatacgagg aattggtgaa aatttcgttg gtggtgctgc cggaggtgta
    34081 gctgaccaaa tgatttccaa atctatgagt ggtcaacgaa gtataactac tacacctctt
    34141 atagagcgag taggaaaaaa agtttcagct atgacagaat taccggcaac gattgaaagt
    34201 agggcagaac aatggaaacg gacaattcag tatgtcccaa ccaatgccca atacaaagca
    34261 caaaaaatga aatacgatta tttctctggg cgaatgaatg agtcggaacg acaaacacga
    34321 gcgcgtaacc ttgcgaatga gcgacagaag aatttttatg aagcacgaaa agatgcattg
    34381 caattagagc aacaagagag aaaacaacaa gcgatgcgtt caatggcgca tcaaacagga
    34441 aaaacacaag tgacttctgg taaattacaa acacggaaaa gtgcaatgat tggtcgaaca
    34501 gaacaatttc ctacgataga aaaaagaagt aacatttcaa atgtagctaa aaataagaaa
    34561 ttgtcgaagc aggaacatcc gattcatgaa cgaagtacga atcgaaagat tgaaactccg
    34621 gttcgttgga cttcgacttc ttgtcaaaac gcgagaaaag gtagaactaa aaaagaataa
    34681 ggaggagggc tatgtttcct cggaagaaaa aactgtctat tctctctgaa caagaaacaa
    34741 gactaaaagg aaagaaagac aagaaaaaag aaagctataa gggagcgcga aaaaggcgct
    34801 tccttttttt tgtaggctgg agctttttgg tttttagtgt ttgttttgga gtttataata
    34861 atttaacttc tcaagatacg catacaatcc atgaaacaaa agtcattgat aaacaattaa
    34921 aagatatgac tggaatagaa agttatgtga aaaattttgc atttttgtat tttacagtga
    34981 gtcttgaaag cgaccaacaa ataaatcgat caaaagcagt agaaagttat ttagctgatg
    35041 ggctgcaaat ggataatgaa atagataaag acatagaaac gaatgtaaaa gttaaaaatg
    35101 tctcgatttg ggcaatagag gaacatgaat caaaaaaaca tacatttgtg gttatatttc
    35161 atgtcttatt agagatagaa aaagaattca aagagcgtgt gtataaggtg gaggtatatc
    35221 gaaacgaatt atcatatgcc atagtgaaat tgcctattgt agcgagtaat gttaaaaaag
    35281 caacgattaa agtagacact cggttcgaaa caacgagcat ttctcgaaaa gaacaggaag
    35341 aattagagaa attcttatat accttctttc gtatttatcc taaagcaaac gcagaagaat
    35401 taaaatatta tgggacaact cttttaccaa ttcaacagcc tttagccttt gaagaactat
    35461 cgaatttgca agtcgcaaaa aaagagggta gatatgagat tcattgtaat attttgtatc
    35521 aagatcaaga aacgcacctt tggtttacca tgtactatga aatgtctatg aaacaattag
    35581 agaatgggaa ctttcgaatt gaggcgattc actagtgaaa aagatttttt tatttagtgg
    35641 catctgtatg atgagttgcc tgtttttatt atttgcgata attagcatgt ctagtggtga
    35701 atcgaagcac atggattcag cagttggtgt tactcatacg ggagaatata tcgaaacctt
    35761 acctattttt tcagagatca aagggcgtgg tccgataaca gatgaacatg cccaagcagc
    35821 agttggtgca gctgtaaagt atcagttatt accgagtgtg attctttctc aattaggata
    35881 tgaatcagct tatgggcagt cacaatctgg aagaaacgat acgaactttt ttgggattac
    35941 gtggtttcct ggttgtccct atccacaagg ttctgcacgt gggattggtg gctctgaagg
    36001 tggtttctat atgaagtttc cgaacctgaa tgcttgttat gactattatg gatttatggt
    36061 ggcgagtcaa agcaacttta atcaatgtgt gggcaataaa gatcctggaa gttgtttatt
    36121 gattctaggt cgtggaggtt atgcggcggc aggtatcacc gaaggcagtc cgtattatca
    36181 aggcgcaatg agtatcataa agtcctataa cttaacggaa tatgatgcct ttgcgatcaa
    36241 gcattggaat agcctaccaa ccaaaaaaac ttcgcttatt gcaggaaaaa gtaaaggcaa
    36301 tgcgaaggtt ttggatcgga tcattggacg acccgtaaat aatggggaat gttatggtgg
    36361 tacggcatgg tatgtatctc aactaggtgg cccacagtta atgggttctg gacacatgtt
    36421 tgcgatgtta ataggggaag attataactg ggcaacctat ggttggcaag tgatcatgaa
    36481 tccaaagccc agtgacttac aagcaggaga tgtggtcaat tggcaagcag gtggtgcatt
    36541 gtctcctgga atctatggcc acacgggtat cataacagat gtctcaaata gtggacaaag
    36601 tttctccacc gtagaacaga acgccgagca ggggcgaatt atggcacggt atcaacgcag
    36661 ctataaccaa gcgaaaattc gaagtctggt tcggaaagtg aaataaaaga attctttcga
    36721 tgaaaatttt tagtataaaa gggagaagga tcgattgtga aaaattatta ttttttcttt
    36781 ctaagttgtt tttgtttgtt tggacttact agttgtgggt ggattttttc tgaagaaaaa
    36841 acagaagttt ccttaatatc aaaagaaaag atgatacaag cagaaagttc aagtaaagaa
    36901 agcaaaagaa atcaggaaga caaaacagaa tgcaatattt ctgaagaaat ccaatatttg
    36961 ttaaagaatt ttggaagaaa atgggtgaat tacacggata tatacgaacg aaatcaatct
    37021 gtaaaaaact atttaaccga tcgttgtata aaagaaaatg ggattgacaa cgatccacat
    37081 gtacaatata aaacaattgg ccgtattcat actatcacac aagatgtaat ccaatcggat
    37141 caatatttat tatttggaga agaaacaaca aatgagatga ctcggtttgt gttacttcaa
    37201 atcaaggtgg atcaagaagt gcagaagatt gataaaatca agatttatta tgtaagaagt
    37261 gcatattaaa agaaaaatga cgttgtttta tttactagaa attatagttt aacgaaaagg
    37321 aagaagctga actatttaag atgtttaaag aatatgaaga attttgggaa taaatcaatt
    37381 aaaaggaaag agataagaaa ataatgtaca aaaaatagga ggaggaatga gatagaaacg
    37441 gaaaaagtag aagtactttg gttaaaacaa gtactagagt tgaaaaatgt ctttttgaac
    37501 catgatttag tgaatcatgg gatgaatttg tgaaaaaatg atcctaagta gatacacgga
    37561 cgcaaatttc tatcgctaca tacaaaagcg gatgagcttg tagagcaaat ttataatcag
    37621 ttagatgtaa tttcagaaca attgattatg atcgatgggt tcctgtataa actctatagg
    37681 aaacacagtt gaattggata aatagattca gaatttcaaa gcacctggtt atcttgatta
    37741 ttctaatata catttaaagg ggcaactcct tcccctgagc tacccctaga gagtgatata
    37801 ggaatattcc caagtctatt ataagaatac ctacacgtta tctcaacaaa tttgctctta
    37861 cactcatagc ttttacctta taaaattaac gttcattacc attaaaactt tcagaaaaat
    37921 aatttatact tttatttttt atcgaaaaaa attctaaata gagtattttt catttccgaa
    37981 caaattatga tcctttcttg acaaatatag aagctacaat gtttactagt tttgaataag
    38041 aaagatagtg ctgtgtcttt tataattgat tgtttgattt ttcatgaaaa aatgctcgag
    38101 ttaaaaaaac aaattgaacg ttcattttat ttgacttagt agctccttct tgttaacatt
    38161 gtttcataaa ccatttaatt ttataaatta agtatgtctt tatctttctg aaatgtgaga
    38221 tgctacttt
//
LOCUS       DAXWOO010000007        37620 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.007, whole genome shotgun sequence.
ACCESSION   DAXWOO010000007 DAXWOO010000000
VERSION     DAXWOO010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37620
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.007"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            64..660
                     /locus_tag="ACGE70_000298"
     CDS             64..660
                     /locus_tag="ACGE70_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFF8691969.1"
                     /translation="MNPTLITKKELLKKLDISTGVLANLIRNGMPKEGEMFNLDKIIT
                     WRENWSKNILGELEVGRVYTNKEISEKFKCSKQGGMRRSHQTNTLVLFSDQTGSNVYK
                     DKWLNGILQYTGMGLKGDQVLDKNQNKVLANSKSNFVKIHLFETFKPKEHTYLGEVYL
                     AGQIYTVNEKDSSGNSRKVYKFPLALINQEQLIEDKDI"
     gene            641..1087
                     /locus_tag="ACGE70_000299"
     CDS             641..1087
                     /locus_tag="ACGE70_000299"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFF8691970.1"
                     /translation="MKIKIYNQEENQTRHIRNLSNAKLEEEARKVSNYNKKMAAKYVN
                     EEEFCFSKTKIYKRNPFVSEYVKRLAKGICQLCQEKGPFIKDGVPYLHCHHIEYLSQG
                     GKDVIENCIALCPNCHARIHELELPDDKEKLLKIVEKRENSFSKIP"
     gene            1430..1753
                     /gene="relB"
                     /locus_tag="ACGE70_000300"
     CDS             1430..1753
                     /gene="relB"
                     /locus_tag="ACGE70_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFF8691971.1"
                     /translation="MRFFKRGNYRAMITVRVSDAKKEWLSYMAEFYGITLSELLKNYS
                     MEQLEDEYNRQTAEIAHKHWIRSGKTTVFMEESLMNLVGWNDLPFKIHARNSEAALIG
                     KYVYW"
     gene            2073..2300
                     /locus_tag="ACGE70_000301"
     CDS             2073..2300
                     /locus_tag="ACGE70_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFF8691972.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            2316..3482
                     /gene="hemW"
                     /locus_tag="ACGE70_000302"
     CDS             2316..3482
                     /gene="hemW"
                     /locus_tag="ACGE70_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFF8691973.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            3717..4760
                     /gene="hrcA"
                     /locus_tag="ACGE70_000303"
     CDS             3717..4760
                     /gene="hrcA"
                     /locus_tag="ACGE70_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFF8691974.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            4854..5417
                     /gene="grpE"
                     /locus_tag="ACGE70_000304"
     CDS             4854..5417
                     /gene="grpE"
                     /locus_tag="ACGE70_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFF8691975.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            5468..7297
                     /gene="dnaK"
                     /locus_tag="ACGE70_000305"
     CDS             5468..7297
                     /gene="dnaK"
                     /locus_tag="ACGE70_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFF8691976.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            7448..8614
                     /gene="dnaJ"
                     /locus_tag="ACGE70_000306"
     CDS             7448..8614
                     /gene="dnaJ"
                     /locus_tag="ACGE70_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFF8691977.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(8982..9719)
                     /locus_tag="ACGE70_000307"
     CDS             complement(8982..9719)
                     /locus_tag="ACGE70_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFF8691978.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(9721..11223)
                     /locus_tag="ACGE70_000308"
     CDS             complement(9721..11223)
                     /locus_tag="ACGE70_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFF8691979.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            11482..13476
                     /gene="uvrB"
                     /locus_tag="ACGE70_000309"
     CDS             11482..13476
                     /gene="uvrB"
                     /locus_tag="ACGE70_000309"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFF8691980.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            13486..16302
                     /gene="uvrA"
                     /locus_tag="ACGE70_000310"
     CDS             13486..16302
                     /gene="uvrA"
                     /locus_tag="ACGE70_000310"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFF8691981.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            16565..17830
                     /locus_tag="ACGE70_000311"
     CDS             16565..17830
                     /locus_tag="ACGE70_000311"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFF8691982.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            17834..18565
                     /locus_tag="ACGE70_000312"
     CDS             17834..18565
                     /locus_tag="ACGE70_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFF8691983.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            18700..19584
                     /gene="rapZ"
                     /locus_tag="ACGE70_000313"
     CDS             18700..19584
                     /gene="rapZ"
                     /locus_tag="ACGE70_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFF8691984.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            19581..20579
                     /gene="yvcK"
                     /locus_tag="ACGE70_000314"
     CDS             19581..20579
                     /gene="yvcK"
                     /locus_tag="ACGE70_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFF8691985.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            20613..21548
                     /gene="whiA"
                     /locus_tag="ACGE70_000315"
     CDS             20613..21548
                     /gene="whiA"
                     /locus_tag="ACGE70_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFF8691986.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            22116..22772
                     /locus_tag="ACGE70_000316"
     CDS             22116..22772
                     /locus_tag="ACGE70_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8691987.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            22951..23516
                     /locus_tag="ACGE70_000317"
                     /pseudo
     CDS             22951..23516
                     /locus_tag="ACGE70_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(24215..24736)
                     /locus_tag="ACGE70_000318"
     CDS             complement(24215..24736)
                     /locus_tag="ACGE70_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFF8691988.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            24934..28614
                     /gene="nifJ"
                     /locus_tag="ACGE70_000319"
     CDS             24934..28614
                     /gene="nifJ"
                     /locus_tag="ACGE70_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFF8691989.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            28803..29690
                     /gene="cdaA"
                     /locus_tag="ACGE70_000320"
     CDS             28803..29690
                     /gene="cdaA"
                     /locus_tag="ACGE70_000320"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFF8691990.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            29687..30856
                     /locus_tag="ACGE70_000321"
     CDS             29687..30856
                     /locus_tag="ACGE70_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFF8691991.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            30880..32235
                     /gene="glmM"
                     /locus_tag="ACGE70_000322"
     CDS             30880..32235
                     /gene="glmM"
                     /locus_tag="ACGE70_000322"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFF8691992.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(32307..34112)
                     /gene="glmS"
                     /locus_tag="ACGE70_000323"
     CDS             complement(32307..34112)
                     /gene="glmS"
                     /locus_tag="ACGE70_000323"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFF8691993.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            34522..34812
                     /locus_tag="ACGE70_000324"
     CDS             34522..34812
                     /locus_tag="ACGE70_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8691994.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            34825..35850
                     /gene="sppA"
                     /locus_tag="ACGE70_000325"
     CDS             34825..35850
                     /gene="sppA"
                     /locus_tag="ACGE70_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFF8691995.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            35853..36512
                     /locus_tag="ACGE70_000326"
     CDS             35853..36512
                     /locus_tag="ACGE70_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFF8691996.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            36669..37601
                     /locus_tag="ACGE70_000327"
     CDS             36669..37601
                     /locus_tag="ACGE70_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8691997.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
BASE COUNT        12361 a         6456 c         8191 g        10612 t
ORIGIN      
        1 tttttttcga tttttcaagt tatctaatgc aattatattg atatcattag gagctgcatt
       61 gttatgaatc caacattgat tactaagaaa gaattactaa aaaaattaga tatttctact
      121 ggtgtattag ctaatttaat tagaaatggc atgccaaaag agggagaaat gtttaatctt
      181 gataagatta ttacatggcg agaaaattgg tctaaaaaca tattaggtga gttagaagtt
      241 ggtagagtat atactaacaa agaaatttca gagaagttta aatgttccaa acagggtgga
      301 atgagaagat ctcatcaaac taatactctt gtactgttta gtgatcaaac aggaagtaat
      361 gtttataaag ataagtggtt aaatggtatt ttacaatata ccggtatggg gctaaaaggg
      421 gatcaagtat tggataagaa tcaaaataaa gttttagcca attcaaaaag taattttgta
      481 aagattcatt tatttgaaac atttaaacca aaagaacaca catatctggg agaagtatat
      541 ttagcaggac aaatatatac tgttaatgaa aaagatagtt cgggcaacag cagaaaagta
      601 tataaatttc cattagcttt aataaaccaa gagcaattga ttgaagataa agatatataa
      661 ccaggaagaa aatcaaacta gacatattcg taatttatct aatgctaaat tagaggaaga
      721 agcaagaaaa gttagtaatt ataataaaaa aatggctgca aaatatgtta acgaagagga
      781 gttttgcttc tcaaaaacaa aaatatataa acgtaatccc ttcgtatctg aatatgttaa
      841 acgacttgca aaaggtattt gtcaactttg ccaagaaaaa gggccattta taaaagacgg
      901 tgtaccttat ttacattgtc atcatataga atatttgtcg caaggtggaa aagatgtgat
      961 tgaaaactgt atcgcgttat gtcccaattg tcatgctaga attcatgaac ttgagttacc
     1021 tgacgataaa gaaaaattgc tgaagatcgt tgaaaaaaga gaaaatagct tttctaaaat
     1081 accgtaattt atttttatag gacaaataag ctaatgtgag ctatgccttc tatataagac
     1141 taagttctat tataagtaac gaagtaattc tcgttccttc ctgtagtaat attttttaaa
     1201 taacctatag gcttcttcaa aagtacatcc tccagtaaat tctaagtact ggaggattct
     1261 ttttctagtg ttgctttact catgaaaaag tagttggcta cttcatcaaa atttctgaac
     1321 ttttttaatg tataataaag agctcgatca tttactacaa ggatgctagc tcctacattt
     1381 tcacagtttt ttagcaagta aatttttcta aaatcacatc aggaaatgca tgagattctt
     1441 taagagaggc aattataggg ctatgattac ggttcgtgta agtgacgcca aaaaagaatg
     1501 gctttcttat atggctgaat tttatgggat tactttatct gaacttctaa aaaactattc
     1561 gatggaacaa ctagaagatg aatacaatcg tcaaacggct gaaattgccc ataaacattg
     1621 gataagaagt gggaaaacaa cagtattcat ggaagaatcc ttaatgaatt tggtgggctg
     1681 gaatgacctg ccgtttaaaa ttcacgcccg aaattcagaa gcagctctga ttggcaaata
     1741 cgtgtattgg tagagataga agataatcaa ttcgttgtta tcgctattca agtagggcac
     1801 cgaagaaatg tctatgatta agagactgtt tatttttaca aaaaaataat aattggaatc
     1861 tagttaaaac tcttgaagtg agagatttga ctagattttt ttatgaataa aataaggaca
     1921 aaattgttta ggaagaatca tgagtgtaaa aaaatcatcc aagaagtcga ttcttggatt
     1981 ttttagtgga tttgttcgcc aaatacgttt agtatgcgta tatgagaggt tgtaagcaaa
     2041 gcgttgtggt ctgagcactt aaggaggaat ctgtgaaaat agcttttcat atttttgcag
     2101 attttgactt aatgccgaag gtcagctttg cgattaccgt ttatcgagag gctgtaaaag
     2161 caccgtgtag ctccgatgac aataagcaag catttttaga gctaggtttc cttgattcta
     2221 aatatgcggc ttatttcaat gagttggtgc ttgaacgcga ttttaagaag gtcgaaaaca
     2281 ggcacgactt ttcgacgtaa ggagaaagaa tttcaatgat agaaacaata aatcccgcag
     2341 aaaacaaggt ttctgcgtac attcatatcc cattttgcga gcatatctgc tattactgtg
     2401 actttaataa agttttttta gaagggcagc cagtagatga gtatgtggaa atgctgctga
     2461 atgaaatgag attaatgctg gaaagtcatc ctacagcgaa tttggaaaca ctttatgtag
     2521 gaggcggaac accaacttct ttatctgcta aacaacttga tcgactatta tcaggtgcta
     2581 gagagatcct gccttttcaa aatggtaatg agtttactgt agaagcgaat cctggtgatt
     2641 tgacccgtga aaaacttcaa gtgatgaaaa actatggcgt caatcgttta tccatgggag
     2701 tgcagacatt tgacaatcgc ttattgaaaa aaattggacg taaacatact gcagaagatg
     2761 tgtatcaaac aatgcaattt ttagaagcag aaaattttac caatgtaagc atcgatttga
     2821 tttatgcatt accgggacaa acactagaag gatatcggga tactttggaa aaagctttgg
     2881 ctttggatct gcctcactat tctttgtact cattgatcct cgaaaacaaa acaatgttca
     2941 tgaactgggt acgacaagga agactcgaac tgcctgatca ggaaacggaa accagaatgt
     3001 ttgaagagac gattgaagca atggaaaagc atggacgtca ccaatatgaa ataagtaact
     3061 ttgggctctc cggacatgaa tctcaacata acctcatgta ctggaacaat gaccactatt
     3121 ttgggtttgg agctggtgct agcggttatc ttggagatat tcgttaccgc aacaaaggac
     3181 caatccaaca ctatttacgc ccattacgag caaatgaacg cccggtattg gaagaagaaa
     3241 aattgacacg aaaaaatcaa atagaagaag aaatgttctt aggactaaga aaaaaaacag
     3301 ggattactaa aaaacatttc tatgatcgct atcaaaaaac aattgaatcg gtgtacggtg
     3361 atgtgctaag ggaattagag caagaagggt tacttgttaa tgaagaagac cgtgtttatt
     3421 tgacaccaag agggactttt ttggggaaca atgtgtttga gcgcttttta tttgaaaaat
     3481 agtttgaaga atatttgttt tttatcgaaa cttgaaaaga aaaagtttag aaagtttgac
     3541 aatgaggtat tgaagtattg atataactgg atttacatca attatttaga attagcactc
     3601 ttgtgtttag agtgctaaaa aatggagtct ttctcttgac aggagaaggg cttcttgcta
     3661 tatttataag tgaattagca cttagaatat atgagtgcta atgagaggtg aggaagatgt
     3721 taacaaagcg acaaaacgac attttacgtc tgatcatcca acactatacg agtagtggcg
     3781 tgcccgttgg ttccaaaaca ttaatgtctg agggagtcaa ggccagctca gcaacgattc
     3841 gcaatgatat gaaagcatta gaagatgaag gattgctgtt gaagactcac tcttcttctg
     3901 ggcggattcc atctgcttta ggctatcgtt attacgtgga tcatttgtta aaacctgctc
     3961 gcgtagcaga agatgatttg ctgctgattc gtcagtctct aggcagagaa tttcatgaaa
     4021 tcaatgagat tattaagcaa tctgctgaga tattatctga gctgacaagc tatacgactt
     4081 tttcattggg acctgaaata aaagaccgcc gactgactgg ctttcgtatc gtacccttga
     4141 ataatcgaca gattattgct atcgtcgtaa cagatatggg gaatgtagaa agccaagtgt
     4201 tcactcttcc cgaatatatg ggaagtcagg aactagaaaa aatggtacag attgtaaacg
     4261 accgtctagt cggagatcct ctgatgacgg tgtaccataa acttcgtacg gagattccga
     4321 tgattcttca taaatatttc cagacgacag aagggatgtc tcagttattt gacaccgttc
     4381 ttggtcatgt ttttgaagaa aaggtttatg tcagtggaca aatgaatctc ttggattatg
     4441 agccccgtca agatcttgat cagttcaaat cgatgttttc ttttatgaaa gactcagacg
     4501 gacttacaca aatgatcact ccgatggata gcaatattca tatcaagatt ggtgctgaat
     4561 taggcaatga tttattgcag aatatgagta tgattcaagc aagttacgag atagatggtc
     4621 atggaagagg tacaatagcc ttgttagggc ctactagtat gccttattct cggatgttcg
     4681 gtctagtcga tgtttatcga agagaactag cagctaaact tgctgattac taccgctcgc
     4741 ttgacttgtc aaactcgtaa tgtgaataac gaagctattt gcagctgatc gttctctctt
     4801 tcttagtaag agcaatcaaa cagctgactg aataatgaaa ggaagactgt atcgtgaaag
     4861 aaaatcaaga agaactagat aaagaaatga cagatgctca gccagaaccg gaaatcgatg
     4921 tcgaagctgc agaagattcg ggtatttcag aagctgaagc ggaagaattc gaaacagcga
     4981 aattaaaagc tgagttagaa gaaatggaag ataaatactt gcgggctcgt gctgaaatcg
     5041 caaatatggc aaatcgagga aaaaatgagc gtgagcaatt acaaaaatac cgttcacaag
     5101 atttagccaa aaaattactt ccatcaattg acaacctgga acgtgcatta gccacagaag
     5161 tgtcagatga tcaaggggca ggtttgaaaa aaggcgtgga aatggtcttg gaaagtttgc
     5221 gtaacgcact ggaagaagaa ggaattgaaa aaatccctgc taaaggagaa gcctttgatc
     5281 ctaatcttca tcaagcagta caaactgttc cagcgactga agatacacca gccgatacga
     5341 ttgtagaagt actacaagaa ggctacaaat tacatgatcg cgtgttaaga ccaactatgg
     5401 tcattgttgc acaataattg tgcggatata aaacttacac tttcaattaa agaaggagac
     5461 gttaattatg agtaaaatta tcggtattga cttaggtacg actaactctg cagtagcagt
     5521 attggaagga aacgaagcaa aaatcatcgc taacccagaa ggaaaccgta caacaccttc
     5581 tgttgtatca ttcaaaaatg gagaaatcca agtaggggaa gttgcaaaac gtcaagctgt
     5641 gacaaaccca aatacgattt catcaatcaa acgccatatc ggtgaagcag gttacaaagt
     5701 agatgtagaa ggtaaatcat atacaccaca agaaatctca gcaatgatcc ttcaatatat
     5761 caaaggattc gcagaagact atttaggtga aaaagtcgaa aaagcagtta tcactgtgcc
     5821 agcttacttt aatgatgcac aacgtcaagc aacaaaagat gctggtaaga tcgctggtct
     5881 agaagtagaa cgtatcgtca atgaaccaac tgcagctgct cttgcctatg gtttagataa
     5941 aacagatcgc gacgaaaaaa tcttagtatt cgaccttggt ggcggtactt ttgacgtatc
     6001 tatccttgaa ctaggagacg gtgtctttga tgtattgtca acagctggcg acaaccatct
     6061 aggtggggac gactttgata acaaaatcat cgactacatg gtagcagaat tcaaaaaaga
     6121 aaacagcatt gacttgtctc aagacaaaat ggctttacaa cgcttgaaag acgctgctga
     6181 aaaagcgaaa aaagatttat ctggcgtatc aagcacacaa atcagcttgc catttatcac
     6241 agcaggagaa tctggaccat tgcatttgga aatgacattg acacgtgcaa aatttgatga
     6301 attaacagct gacttagtag aacgtacaaa agtaccagta cgtcaagcgt taaaagatgc
     6361 aggtctgtca caatcagaaa tcgacgaagt tattttagtc ggtggatcta ctcgtatccc
     6421 tgcagttgta gaagcagtac gtaaagaaac aggaaaagaa ccaaacaaat cagttaaccc
     6481 tgatgaagta gttgctatgg gtgctgctat ccaaggtggc gtgatcactg gtgatgtgaa
     6541 agatgttgtt ttacttgacg ttacaccatt gtcattaggt atcgaaacaa tgggtggagt
     6601 gttcactaaa ttgattgacc gcaatacaac gattccaaca agtaaatcac aagtattctc
     6661 aactgcggca gataatcaac cagcagtaga catccatgta ttacaaggtg aacgcccaat
     6721 ggctgctgac aacaaaactc taggaagatt ccaattgaca gatattccag cagctcctcg
     6781 tggtattcca caaatcgaag taacattcga tattgataaa aacgggattg taaatgtatc
     6841 tgcgaaagat ctaggtacac aaaaagaaca aaaaatcacg atcaaatctt cttcaggtct
     6901 aacagacgaa gaaatcgaac gtatggtgaa agatgcagaa gcaaacgctg aagcagataa
     6961 agctcgtaaa gaggaagttg acctacgtaa tgatgtagac gcattgctat tctctgtgga
     7021 caaaacattg aaagaattag aaggcaaagt ggacgaagaa gaagtgaaaa aagcagaaac
     7081 agctcgtgac gaattaaaag ccgctgttga agcaaacaac atcgaggaaa tgaaaactaa
     7141 acgcgatgca ttgaacgaaa tcgttcaaaa cttgactgtt aaactatacg aacaagctgc
     7201 acaacaacaa gctcaagaaa atccagaagc agctcaaggc ggagcagatg atgtcgttga
     7261 tgctgatttt gaagaagtcg atggcgacga taaataatca gttcactgaa tttcgagaga
     7321 aggccaaagt tgctactttg gctttttctt ttgaaaaata gtttgaagta tcggtttttg
     7381 tttcaaacaa aatatgatat gataacaagc gacgtgaaaa tgatgagaac caataggagg
     7441 aaagcctatg gcaacaaaac gtgattatta tgaagtctta gggctctcaa aaggagcatc
     7501 agaagatgaa ataaagaaag cttatcgtaa gctttcgaaa aaataccatc ctgacatcaa
     7561 taaagaacct gatgcggaag aaaaattcaa agaagtatca gaagcatatg agattttaag
     7621 tgacccgcaa aaacgcgctg catacgacca atacgggcat gcaggcacag atccaaatta
     7681 cggtgctggt ggagctggtg gcggatttgg tggattcggc ggcttctctg gtggcggctt
     7741 cggtggtttt gaagatatct tcgactcttt ctttggagga ggcggtcggt cagtcgatcc
     7801 aaatgcgcct cgtcaaggtg ctgatttgca atatacggtc gacttgtctt ttgaagaagc
     7861 gatttttggt gtagaaaaag aaatcaagta taatcgggaa gaaatctgtc atacttgtgg
     7921 tggaaacggt gcaaaaccag gcacacaacc aactacttgt cataaatgtc atggtgcagg
     7981 tacgatcaat gtagaacgcc aaacgccact tggacgagtg atgagccgtc aaacttgtga
     8041 tgtttgtcat ggcacaggga aagaaatcaa agaaccttgt ccaacctgtc atggctcagg
     8101 acatgagaag aaagcacatt cagttaaagt gaatgtacca gctggtgtag aagaaggaca
     8161 acaaatgcgt ttagctggtc aaggagaagc tggagaaaac ggcggaccat ttggtgattt
     8221 gtatgtggtt tttcgtgtag aagaaagtga tatctttgat cgtgaaggtt ctgaaattta
     8281 ctatgaattg cctttaagtt ttgtacaagc agctttgggg gatgaagtcc aagtaccaac
     8341 tgtccatgga gatgtgaaat tgaagatccc tgctggaaca cagacaggta cgaacttccg
     8401 tttacgtgga aaaggcgctc cgaagttacg tggtggtgca acaggcgacc aacatgtcaa
     8461 agtaaaactg atcacaccga aaaatctaaa tgaacaacaa agagaagcat tacgtgcatt
     8521 cgctgaagct ggcggcatca aagttgaaga acagcaagaa gacggtttct tcgataagtt
     8581 taaagatgct tttggcggga aaaagcggaa gtagtttttc tagtaaaaat ataaagtaga
     8641 cttgcccaaa ttttctattt ctagtaaaaa cgctaagtca ttaataatta aaaaccagac
     8701 gaacaatcat acgttcgcct ggtttttttg tagttgtcat gtttgtacca acaaaaaagc
     8761 cagctttaga ataagataat catcatagtt tctgaaggtg gtatcgacta tgtacttgat
     8821 atcgtcaatg aaccgatggt actttcttat gcgatggaac aggtagagcg attgcttgtt
     8881 tgtgcaggag aacaagcgat gcggctggta cagttaagtg aaaaaacaag aaactaaaaa
     8941 tgcccggtcg tcatgaagcg accgggcatt tttggttttt attaaatatt taatacttta
     9001 ttcaagaaat cttttgtccg ttcattttgc ggattttcaa agatttgttg aggcgttccg
     9061 tcttctaaaa agtttccgtc gtcaatgaac atgacacgat tggccacttc tttagcgaag
     9121 cccatttcgt gcgtaacgat aaccatcgtc ataccttgtt ctgctaactt tttcatgacg
     9181 ttcaagacat caccgaccat ttcggggtcg agtgcagatg taggctcatc aaaaagcatg
     9241 atgtcagggt tcatcgctag agcacgagca atagccacac gctgcttttg tccaccggaa
     9301 agcatttcag gatacatatc ttttttatct tctagaccaa ctgtctgcag caatttgatt
     9361 gcttttttct ctgcttcttc tctagattct ttcttcaggt ccatcggtgc taaaatgatg
     9421 ttttccaaaa tagaaagatg agggaatagg ttaaagtgct ggaataccat tccgatatgt
     9481 tgtctcacca agttgatatc tgtgctttta tccatcaagt gggcgccgtc aataataatt
     9541 tcaccgtcag aaggttcttc taaacgattc aagcaacgca aaaatgtact ttttcctgaa
     9601 cctgaagggc caatgataca gacgacatct ccttcgttga tggacacatt gatgtcgttt
     9661 aatactgtgt tatcgccgta ttttttgact aaatgctcaa ctaagatttt ttctgccatc
     9721 ttacttcacc ttcttttcta aaacttttgc taattttgtc aatgctgtga tcaaaatcaa
     9781 gtacatgatc gcaatgatca agtaaacata cgtactttgt gttgtacgtg caacaatgat
     9841 cttaccagtt tgcagcaatt cgactacacc gattgcggaa atgatcgttg tgtcttttaa
     9901 tgagataacg aactgattga cgaatgatgg gatcatgatg cgtatcgctt gcggcaaaat
     9961 gattttttgc attgtccgat tataagaaag acctaaactt ctggaagctt ccatttgtcc
    10021 gacaggaacg gcgttgatcc cgcctcgaac aatctctgcg atataagcac ttgcgttaag
    10081 agtcaaggtg ataacaccag caaggaagtc aggaatcgta aatccaacga taccaggcaa
    10141 cccaaagaaa ataaagaacg caagaaccat cattggaatt cctcggatga tatcgacata
    10201 aatactagca attgtgcgaa gagcgcgtat tggtgctaca ctgaataatc caaaaatcac
    10261 accgattact atagccaaag caaaggagat caaagttagg agaattgttc tccaaagtcc
    10321 tttcaacaat gcttgatagt tgttttggat aagtccaaga aatgttgatt catcagcagt
    10381 ggaagactca ctggaagttt ctgaaccatc cgctacatat tctttaataa tcttatcgta
    10441 ttcgcctgtt ctttttaatt ctttcaatcc ttcattgaac atattaagaa gctcaggatt
    10501 ttgtcctttt ttaacggcaa atgcatattt tccgccagat tcttttttga tcggtgtttc
    10561 caattcttgt ccttgtttga tcgcataacc gattaccgga taatcatcca tcattgcatc
    10621 gattgcgcca acttttaacc cgtcgtacaa ttgatcagca gcatcaaaat atttgataga
    10681 atagccatat tcagattcat ggtcgcttaa aaattcagca ctttcggtac caacttttgc
    10741 tccgactgtt tttcctttca aatcttcata agacttgatc gaagtatctc cttttttgac
    10801 agctaattgg atcccgctat caaagtaaga atcagaaaaa tcatagcttt tctttcgttc
    10861 atctgttaca gtcattccgg caatcattcc gtctgcttgt cctgattcca aagcttgcaa
    10921 agcagcagag aaaccacggt agtccattcg gatgttaaat ccttgcagtt tggcgatgtt
    10981 ttccatcaat tttacatcga tgccttcgta ctcgcccttt gcgttttgaa attcaaatgg
    11041 agcaaaagca gagtcactgg caattacgta tacgtctttt ttgggctcta ttttttgcat
    11101 tgtctcgctt gtgcttgact ctgccgttgt agtgttgtca gcttgtgcaa catgtccaaa
    11161 cgcaagtgtc acaagtcctg tcattaagaa aacaaatgag tgaaataaat gctttctttt
    11221 cataatcaac ctcctaaatt tccaatattt tattattata ggcgaaaagt gctgaatcag
    11281 acaagactgt aagctcgaaa aaatgaaaaa aatgcgagtt tctaacattt tatgttataa
    11341 aacgtaacat gcataataag ataaaaaagt ggcgaaagta tgttcggtgt ggtataattt
    11401 tttagtaagg aatgcctttg ctttcctttt ctttttattg gtcgtaaaat gaaaaggaat
    11461 gaatgaaaag gaggacgaag aatgattgag agagatacct cccgacactt tgagcttgtc
    11521 tcgaaatacc aaccggctgg tgatcagcct gaagcgatta accaattagt ggatggcgta
    11581 gtcggcggga aaaaagcaca aatcttatta ggtgcgactg gaacagggaa aacatatacg
    11641 atttccaatc ttattgagaa agtgaataaa ccaacactaa ttattgccca taacaaaaca
    11701 ttggctggtc agctgtacgg ggaattcaag gaatttttcc ctaacaatgc tgttgaatat
    11761 tttgtttctt attatgatta ttatcagcct gaagcttatg tgccttcaag cgatacgtat
    11821 atcgaaaaag attcaagtgt aaatgatgag atcgacaaat tacgacattc ggctactagt
    11881 tcattattag aaagaaatga tgtgatcgtc attgcatccg tttcatgtat ttttggatta
    11941 ggttcgcctt ttgagtacca aaaacaagta gtttcgattc gacagggagc agaactagat
    12001 cgcaatcagt tgattcgtga cttagtcagt atccaattcg aaagaaatga tatagatttt
    12061 cagcgcggtc gttttcgagt gcgcggtgat gtcgtggaga tttttccggc ttcaagagat
    12121 gaacgtgcct tacgtgtgga gttttttgga gatgaggtcg aacgtatccg agaagtcaat
    12181 gcactgacag gtgaagtttt aggagaaacg gaacacgtgg caatcttccc ggcgactcac
    12241 tttgtgacga atgatgaaca tatggagcat gccgtagcga atatcaaagc ggaattagaa
    12301 cagcgtttaa ctgtcttgcg aaatgaaaac aaacttctgg aagctcaacg tttagaacag
    12361 cgaaccaatt acgatattga aatgatgtta gaaatgggct acacgtcggg aattgaaaat
    12421 tattctcggc atatggacgg acgtaaagaa ggtgaaccgc cttatacgct attggatttc
    12481 tttcctgaag attttttgat cgtggcggac gaatcgcacg taaccatgcc tcaagtcaga
    12541 ggaatgtata atggtgaccg agcgagaaaa cagatgctag tagattacgg tttccgtttg
    12601 ccatctgctt tagacaatcg gccacttcgg ttagaagagt ttgaaaaaca tgtcaatcag
    12661 atcatttatg tctctgcgac gccaggacca tatgaacatg aacaaacaga taccgttatc
    12721 caacaaatca tccgtccgac agggctgctt gacccagtga tcgaagttcg tccaatcatg
    12781 ggacaaatcg atgatctagt aggcgaaata aatgaacgag tcgaaaaaga ccagcgggtg
    12841 tttgtaacga cattgacgaa aaaaatggca gaagacctca cagattattt caaagaatta
    12901 gggatcaaag tcaaatattt gcatagtgat atcaaaacgc tggaacgaac agaaatcatt
    12961 cgagatcttc gattaggtga atttgatgtg ttagttggaa tcaatttgct tcgtgaagga
    13021 ttagacgtac ctgaagtttc ccttgttgct atcttggatg cggataaaga aggcttttta
    13081 cgtagtgaac gttccttagt ccaaacgatt ggacgagctg ctcggaatga agaaggtaaa
    13141 gtaatcatgt acgcagacaa agtaacagat tctatgcgtc tagcgatgga tgaaacatca
    13201 agacgccgta cgattcagca gaagtacaat gaagaacatg ggatcgttcc aaaaacgatc
    13261 attaaagaaa tccgagattt gatttctatt acgaaagaat cggaagatga tacaaaagaa
    13321 gcggtacaag tctcgtatga agaaatgacg aaagaagaaa aagatacgtt attgatgaaa
    13381 ttagaaaaag aaatgaaaaa tgcggcgaaa gcattggatt ttgaaacagc cgctaatgtg
    13441 cgagatatga ttttagaatt aaaagcttca aagtaggagg acaacatggc taacgataaa
    13501 attacgattc acggtgcacg tgcccataac ttgaaaaata tagatgtaac gattccacga
    13561 gataaattcg tggtcgttac aggtttgtcc ggttctggaa aaagttccct agcatttgat
    13621 acgctgtacg ctgaagggca gcggagatac gtagagagcc tttctgctta tgctcgtcag
    13681 tttttaggac aaatggataa gccggacgta gatagcatcg acggattgag tccggcgatt
    13741 tctattgatc agaaaacaac aagtaaaaat cctcggtcga cagtaggtac tgtgacggaa
    13801 atcaatgact atttacgctt gttgtttgca cgtgtaggac atccgatttg tcccaacgat
    13861 catatcgaga ttaccagtca atcagtagat caaatggcag atcaagtaat ggctttgcct
    13921 gagcgcacaa agatccaagt gttggcaccg atcgtggtaa agaaaaaagg acaacataaa
    13981 aaaatattcg aacgaatcca aaaagaggga tacgtccgcg ttcgagtcga tggtgagatc
    14041 tatgatttaa gtgaagtgcc tgagctggag aaaaacaaaa aacatgatat cgcgatcgtc
    14101 atcgaccgga tcattgtgaa agaagggatt cgttctcgtc tatttgattc tttagaggcg
    14161 gctctgcgat tggcagacgg ctatgctctg gttgacgtga tcggtgaaga agaaatgttg
    14221 tttagcgaac actatgcctg tccttattgc ggatttactg ttggcgaact agaaccaaga
    14281 ctgttctcat tcaatgcgcc ttttggagct tgtccagact gtgatggttt aggtgtcaaa
    14341 ttagaagtcg atcaagattt agtgattcca gatagaacga agacacttag tcaaggtgct
    14401 atcgttccat ggaatccaat cagctctcag tattatcctc aaatgttagc gcaggcttgt
    14461 gaaagtttcg gtatcgattt agatacacca ttcgaagaat taccggaaga gcatcaggat
    14521 atcatcttga atgggacaga tgatcttttc catttccatt atgaaaatga ttttggtggc
    14581 gttcgcgatg tagaaacgac tttcgaaggg gtattgaaga atatcgagcg tcgttatcat
    14641 gatacgaaca gtgatttcac gcgtgagcaa atgcgtcttt atatgacaga attaacttgt
    14701 cagacatgta aaggttatcg attgaatccc caagctctat ctgttcaaat caatggaacg
    14761 aatatcgggc aaacaaatga gttgtctatt caaaaagcag tagatttctt ctcaaactta
    14821 acgttatctg aacaagagaa agtaattgcc aaaccgattt taaaagaagt aaatgacaga
    14881 ctttcattct tggaaaatgt gggactagac tatctaactt tgagtcgcgc ttccggaaca
    14941 ttatctgggg gagaagcgca gcgaatcaga ttggctacac aaattggatc gaatctttct
    15001 ggagttttgt atattttaga tgaaccatcg atcggattgc atcaacgcga caacgatcga
    15061 ctgattggct cattaaagaa aatgcgtgat cttggcaaca ccctcgttgt agtcgaacat
    15121 gatgaagata cgatgtgggc agcagattat ctgattgacg tcggacctgg ggccggagaa
    15181 caaggcggac aaatcgtggc tgcaggcact cctgaagaag tagctaatga tgaaaattct
    15241 ttgacaggga aatatctttc agggaaaaaa tcaatccctg taccaaaaga acgccgtaaa
    15301 ggtaatggga aagcaatcaa gatcactggt gcttcagaaa acaatctgaa aaatattagt
    15361 gttgaatttc cattaggaga atttgtagca gtcacaggtg tgtctggttc tggaaagagt
    15421 acattggtca atagtatctt aaagaaatcg ttagcgcaaa aattaaataa gaattctgct
    15481 aagccaggta aattcaagac aatttccggc tacgaaagta tcgaaaagat catcgatatc
    15541 gatcaaagcc caatcggccg gacgccgaga agtaatccag cgacttatac aagtgtattt
    15601 gatgatatcc gtgggttatt tgctcaaacg aacgaggcaa aaatgcgggg ttataagaaa
    15661 gggcgtttta gtttcaacgt aaaaggcggt cgttgtgaag cttgtcgcgg ggatggaatt
    15721 attaagatcg aaatgcactt tttgcctgat gtctatgttc cttgtgaagt atgtcatggc
    15781 aaacgatata actctgaaac attagaagtg cattacaaag gaaaaagcat tgctgatatt
    15841 ttggaaatga cagtagaaga tgctgtagaa ttcttcaagc acattccaaa gattcatcgc
    15901 aaactgcaaa cgattgttga tgttggctta ggttatgtga cgatggggca accagcaacg
    15961 acattgtccg gtggtgaggc acaacggatg aaacttgcca gtgaattgca caaaatctct
    16021 aatggaaaga atttctatat actagatgaa ccaacgacag gacttcatag cgatgacatc
    16081 gcccgcttgt tgcatgtatt acaaagatta gtagatgctg gtaacacagt tttagtgatt
    16141 gaacacaatc tagatgtaat caaaacagca gattatatca ttgatttagg accagaaggt
    16201 ggagaaggtg gaggaacgat ccttgcgact ggaacaccag aagaaatcat taacgtaaaa
    16261 gaaagttata caggtcacta tttgaaaaaa ataatggtat aaagaggata gatagctgag
    16321 atagttaaaa aaacataaaa aaatgaattt ttgcttgttt tattaatatc agtgttctag
    16381 aatcattaca tggtagttct ttctacactg attggtatag acaaaaactt cctataagtt
    16441 caatccgttc aaaaaagaat tgagcaatcg gaggagtttc ctttgattgc ttttttcttt
    16501 atggtatgat attccttgtg tagttggaaa agaaaaacaa gagattatga gtaagtgaga
    16561 gataatgatg aacagtattg aaaatgaaga atttatccct atattattag gaagcgacat
    16621 gaacgtttat ggtatggcac gttcttttaa tgaagcatac ggcaaaatct gccaagcata
    16681 tgcttcggat cagttagccc ctacccgcta cagtaaaatt gtcaatgtag aagttattcc
    16741 aggatttgat aaagaccctg tgtttatcga gacgatgctt cgtttggcaa aagaaagata
    16801 ttctgataag agtaaaaaat atcttttgat tgcttgtggt gatggatatg cagaattgat
    16861 ctcccaacat aaacaagagc tgtcagaata ttttatctgt ccatatattg attattcttt
    16921 atttgaaaga ctgatcaata aagtaagctt ttatgaagtt tgtgaagaat atgatcttcc
    16981 gtatcctaaa acattgatcg tcagagaaga aatgttagta aatggacatt tggaacagga
    17041 attgcctttt gaattcccag ttgctttgaa acctgcaaac agtgtggagt atctttctgt
    17101 ccaatttgaa ggaagaaaaa aagcttttat tttagagact agagaagagt tcgatttaat
    17161 tttaggccgg atatacgaag ccggttataa aagtgaaatg attgtacaag attttatccc
    17221 gggtgatgat agtaatatgc gtgtgctaaa tgcgtatgta gatgaagatc atcaagttcg
    17281 gatgatgtgt cttggtcatc cactgttaga agatcctaca ccagcatcga ttggtaatta
    17341 cgttgtgatc atgccagatt ataacgaaaa aatctatcaa accattaagg catttttgga
    17401 aaaaatcgaa tatacaggct ttgccaattt cgatatgaag tatgatccta gagatggaga
    17461 atataaatta tttgaaatca atttgagaca aggtcgtagc agtttctttg tgacattgaa
    17521 cggcttgaat ctggctcgtt tcgtcacaga agatcgagtg tttaacaaac cctttgttga
    17581 aacgacctat ggaacgaatc aatcagataa agcaagatta tggatgggtg tgcccaaaaa
    17641 aatcttttta gaatatgctc gggaaaatga agacaaaaaa ctagctgagc aaatgatcaa
    17701 agaaaatcgt tacggtacta ctgtgtttta tgaaaaagac cggtcaatca agcgctggtt
    17761 gttgatgaaa tatatgttcc ataactatat tccaagattc aaaaagtatt ttcatgtgaa
    17821 agaaggctag ttcatggaga attttttcag tattttaggc ggaatgggca cgatggcgac
    17881 agaaagtttt gttcgcttga tcaaccaccg aacgaaagct acaaaagatc aagaatattt
    17941 gaattatgtc ttattcaatc atgcaacagt tcctgatcgt acagcttata ttttagatcg
    18001 atcagaagaa aatccaatgc cgtttctgct ggatgatatt gagaaacaaa atttattgca
    18061 gccgaacttc attgtgttga cttgtaacac cgcacactac ttttttgaag aattacaagc
    18121 ggcaacggat attcctatcc ttcatatgcc aagagaagca gcaaatgaac ttgtccgtca
    18181 gcatacgaca gggagagtag cgattctagg aacagaagga agtatgaaag ctggaatcta
    18241 tgaacgcgaa gtcaaaaatc ttggattcga aacagtgatt cctgatactg ttcttcaaga
    18301 aaaaatcaat tatctgatct atcatgagat caaggaatcg gattatttga atcaagagct
    18361 atattacgaa atcttagaag aagcggtaga gcgcctaaac tgtgaaaaag ttatcttagg
    18421 ttgcacagaa ttgtcattga tgcatgaatt tgctgaagat aaccattatc cagtgatcga
    18481 tgcccaatct attttagcag atcggacgat tgaacgagcg ctagctgaaa gaagcaaggc
    18541 attggataca gcatcggaaa agtaaaaaaa ttggatttgg ggcaaatatc ctaaaagata
    18601 tttggtccaa atctttttgt aaaagaaaaa gaaaactggt aatgatacgc ccggaatgat
    18661 ataattttac tatgcacaat gtgagaggag ttcgtaatta tgacagataa tttgcaatta
    18721 gtcatcataa caggtatgag cggtgcaggg aaaacagtag ctatccaaag ttttgaagat
    18781 atgggctatt tttgtatcga taatatgccg cctcgattga ttccaaaatt ctgggaatta
    18841 atcaaagaat caggcaaagt cacaaaaatg gctttaggag tggatttacg ttctcgttcc
    18901 ttctttgaag aaatccaaaa catgctgatc gaaatcgaaa atacgaactt catcgacact
    18961 tctatcttat ttttagatgc ttcagacgaa gaactagtgg ctcgctacaa agaaacaaga
    19021 cgagcacatc caatggcgat ggacggtttg atcactgaag ggatacgtaa agaacgggcg
    19081 atactagacg acttgaaagc ccaagcttcc gtgatcattg atacgacgac attgacacca
    19141 cgacaattac gtgaaaaaat caatgagtca ttcaaatcaa agggagacca aggatttcgg
    19201 atcgaatttg tatcatttgg ttttaaatac gggttgccga tcgattcgga tattgtgatg
    19261 gatgtgcgtt ttttgcccaa ccctcattat atcccggaac taagaccatt gaccggaaaa
    19321 gatcagccag tctatgatta tgtgatgtcc tttgatgaaa cgcaatcatt ctaccataaa
    19381 tttttggatc ttctggaaac tgtattgcct ggatacatta aagaaggaaa aagcagcttg
    19441 acagttgcaa tcggctgtac aggcggtcaa caccgttctg tggcattgac tgaacgtttg
    19501 gcaaatgctt tagggaaaaa ctataaagtc aatgtgacac atcgggacaa ggataaaagg
    19561 aaagagacgg tgaaccggtc gtgaaaatga aaacctaccg gataagaaaa ccaaagatcg
    19621 tagtggtcgg cggtggcact ggtctgccag ttatcctgaa aagtttgcga aatcaaggag
    19681 cagatatcac agcagtagtg actgttgcag atgatggggg aagcagcggt gcaatcagag
    19741 aatcgatcgc tatggctcct cctggtgact tgagaaatgt cttagtggcg ttatcggata
    19801 tgccacaatt ctatgaagat attttccaat accgtttcaa aaaggaagat cagtttttag
    19861 ccaatcatac gatcggtaat ctgatcattg ccgcagtttc cgagatgcgg ggaagcactt
    19921 atgaggcgat tcaattatta gcaaaaatga tgcatgtaga tggacatgtt tatccttctt
    19981 cagaaactcc tttgaccttg catgctgtct ttaaagacgg aacgactgca gtaggagaat
    20041 ctaaaatcgc gatcgaccgt aaaaccattg accgtgtgta tgtctgcaac acaaatgata
    20101 aaagtgaagc caaagcggct cgaaaagtcg tttcagcaat tatggacgcc gacatggtcg
    20161 ttttgggacc cggaagttta ttcaccagta tccttccaaa tttagtgata ccagaaatcg
    20221 gcgaagcaat gctcaagaca actgctgaaa ccgtctatat ctgtaatatc atgacccaaa
    20281 aaggggaaac ggaacatttc acagatgccg atcatatacg tgttttacac aagcatttga
    20341 atcagccttt tatcgataca gtattggtca atacggaaaa agtgcctgaa gattatatgg
    20401 acccagagat ctatgatgag tatctagtac aagtcgcaca cgacttcaac ggattgagaa
    20461 atgaaggatg tcgagtgatt tccaccgact ttttggaatt gaaagatggt ggtgtcttcc
    20521 acgatggaga caaagtagta gaagaattgt tccgtttagt tttcggttca aaatattaag
    20581 caatttattg ttggagaaag gaggagaata ggatgtcatt tgctgcagat gtgaagaaag
    20641 aactaactgg tttggaagtt catcgagaac atgcgaaagc ggaattagct gctttgctgc
    20701 gaatgaacgg ctctttaggt attgtgaacc agcagtttat tttgaacgtc caaacagaaa
    20761 atgctgcaat tgccagacgt atctattctc ttttgaaaga tcattatcag gtacgaagtg
    20821 agctactcgt tcgtaaaaaa atgaaattaa aaaagaacaa tgtctatatc gtccggttga
    20881 agcaaggcac aaaagatgtg ttgatggatc ttgatatcat ggatggtatg atgttcaatg
    20941 gacacgtttc ggacgagatt atgggaaatt cgcaaaaaat gcgttcttac ttacgtggtg
    21001 cttttatggc ctcaggttca gtcaataatc cggaaacgag ccgttatcat ttggaaatat
    21061 tttctattta tgaacagcat aaccaagaca tttgcgatat gttaaattat tacggtctac
    21121 atgctcgaac gcttgaacgg cgcaatggct atatttctta tttaaaagga gcagaaaaaa
    21181 ttgctgattt cttaacattg attggtgcaa ccaactcaat gctccgattt gaagatgttc
    21241 gaatcgtacg ggatatgaga aattcggtca atcgtctagt caattgtgag acagcaaatc
    21301 tgaacaagac gattgatgcg gcttctaaac aaatcgaaaa catcgaattg atcgaagcaa
    21361 aggtcggtct acatgccctt cctgaaaaac ttcaggaaat cgcagaactt cggcttcaac
    21421 atccagaggt cagcttgaaa gaattagggg aaatgattcc ctcaggtgcg atatcaaaat
    21481 cgggaattaa tcaccgaatt cgcaaaataa atgaatttgc ggaaaaactg cgtgaagaag
    21541 cagtttaatc caaagaaaaa actgatacat gaagcaggag atgcgaatgt atcagttttt
    21601 tttaaaagtt atacttgctt ttcttccgtc aaactataat tccatcttta ctggagtaac
    21661 ataatttgga aaggcttcta ttgtttttaa tacaatcgta taaaaagcgg ggagacaact
    21721 gaatgaagat tttagttttg tacataaatt tttgcttaat atagaagtcc tgatatagat
    21781 aagatcaagt atttatctat aactggcctg attaattaaa aaactggaag gaactgctcg
    21841 atcatccttg agaactgctc ttcttacttc tggtcaagat tctcgtggtt ggtggataag
    21901 gatggttgat aagttcattg gattagctag tcgaaaacaa ccaggtgtaa ttaatgtaga
    21961 agttttaaca aggggatgga gtgatttatt cagtgataat cataagttca taaatagtaa
    22021 ttgagatgta tggttggaat acgttatacc tgaacttgaa ctaccaagat ttggtttaga
    22081 atttgagaat cgatgtttat aaagggaggg caaaaatgcg tttttttaca acttttacta
    22141 tcattatgat tgcagtactt tttatttttc tagatatagc aaagcgaaat acagcatttt
    22201 tattatatcg ggttttactt cgagcaggtc tcataacatt tatatcgatc gttggatttt
    22261 ttctattcac agttattgta tttatttgga gaactccagc accaccattg ccagagatta
    22321 catatggaga atttcctttt agacttgagt atgaattgaa tgaagaatta catgtgatag
    22381 aagacacgtt aatagttgaa tttgatggat ttggaatgaa cgaaggcata gggagatatc
    22441 gtagatggac aagtcgttta gccagcggag aagatttagt gttgctgtta gaagttagtg
    22501 ataataagca aatattttat tttccaggac ctgctaatta ttacatggga gataggttga
    22561 atggttataa tcatactttt cctagtgctt ctttcattga aagggaaaga ggcattacta
    22621 gaagggatat tttacatgac aaagaattat tagaacaatt tggtccgctt gatcaaaata
    22681 caataaatga agaagaattg ttaaatcaat ataatataag gctagtgaat tgggaaatat
    22741 ctgaaccgat tgttaacaat tttggtgatt agaaaacaat tacgataaat agaaacaagc
    22801 caaatcatca gcaagtaaaa gctgatgatt tggcctttct tatacgagta gtaggaattc
    22861 atagcgaata ttattacgat cagtagatat tttgatatta aaggagtatt acgaactagt
    22921 atgtgagaat gtagaggtga tttgcgaata atgagaaatt tatcaaatag aaacaaaata
    22981 ttaatcatta ttgttgttat agctgtgttt catctgggaa cgaatgcggt tttaagtaga
    23041 ataatattgg gtcctaagcc accaagacct gcgattaccc gaggggagtt tgattttaga
    23101 ttagaatatg aagtagatgg ggaaaggatt gtgatagaag atacaattgt tgcattattt
    23161 gatggatttt ctgcagatgc aggtagcatg gcatggtatc gcacgtggcg tttgcatttg
    23221 gcgagtgaca agagggcaca acaattattg ttacatgagt tagatgatgg tcgaaaaata
    23281 tattatcatt ttggaggagc tggattttat atggggaagt tagtagcagt gtaactgtaa
    23341 caaggaaaga tttgccaggt cttggcattc gaaactccat attttatatc gagcgtggga
    23401 gagatacatc tattgggaac aggaaagggt taactcttga agagttatat aatgactttg
    23461 gaatccgtct tgttagttgg gaacacaatc caccaataga aaatcgcttt gaataatcga
    23521 aatctattaa tccatttcat cagccagtga atgttgtaat ggaacaattt ggtttagtgt
    23581 tcaaaaccat tgaaatacta ggctgtgatc agcaaaaatg tatctgaata aaaaagagag
    23641 caactattaa atgagaggag ttaggagttg ttagctttat acgaatcaaa gtggcaaata
    23701 cagatgcgta tgcatttgtg tttgccatct tgcgtttggt agacgagata ggtataagct
    23761 aaagaaacaa cttgtttaaa ataaacgacc tagctcaaca aacgaccatc taatcaacta
    23821 acagcataca taaaagcaac tatttttgaa gtagaattga aatcgggata acttaaaaag
    23881 tgattgggat actagtgtga atcaccgtac ataaatatta ctcgttaagg gaatttgaga
    23941 aattttgtat gaaaatttaa gaattagtta gtatgcgcaa gtcaaaaata tgaataaatt
    24001 atttggtgga gataatttgc tcagcttgtc ataagaaatg agataatctg gcagatgcaa
    24061 gcttttggac accatttatt cggaataagg aggctgggac agaagcgttt aactccaaga
    24121 aataagaagg agttgcttct gcttccgccg tttataggtg tttagagcgt gagacaaaag
    24181 tgttctttac ttttgtccta cgctccttat ttttttattt tgtttggatc aattctttta
    24241 attcctctat tgaaatagat tcatcaaaga tttctttacc caagatgaca gtcggaacaa
    24301 atcgaatatg tgcttgctca gcttcgttga tgacagcttg gctcagctgt tcgtcttttt
    24361 gttctgtcag ctttagttta tctactgcaa aatccgctac ttcttggaga gaaagatgtt
    24421 tccactcgtc ttgagtatcg aatatttgtt tgatctcttt gatcgctttt tgaccatcgc
    24481 tgattgtgag ataccggtgc atcacgtttc ctcgctgcaa gctttctttt tctttatcga
    24541 acaatttgat cactcgctgc aattgcccgc tttggactgc ttcttctaat agctcataag
    24601 attcttcaaa ccattgtcgg caatacggac aagataggtt gatgaattcg accatttttt
    24661 taggagcatt cgatgaacca taaaggattc ctttttgtgt atttgttttc gttgcatcaa
    24721 tcactgatat atccatgtta aaacacctct ttcgcttata gtttatacat gtctgacaaa
    24781 aaaaggcaac gatttagact gtttgtctaa aaattagatg accaattcaa atagacatat
    24841 gtgaaatatt tttataaata aaagaaaaaa tggaatgttt tacgttataa tggaacagat
    24901 aataagagag aatatgaaca aggatgatga atcatgcgaa aaatgaaaac aatggatggc
    24961 aatacggcag ccgcatatat ttcttatgca tttaccgaga ttgctgcaat atatccaata
    25021 acacccagtt cgaccatggc tgaattagta gatcaatggt cagcagcagg aaaagaaaat
    25081 gtattcggtc aaccagtaaa agtagtagaa atgcaatccg aagcaggagc agctggtgtg
    25141 gtccatggat cattgaaaac tggtgcactg acaactacat ataccgcttc tcaagggctg
    25201 cttttgatga taccgaatat gtacaagatt gctggggaac tactgccaag tgttttccat
    25261 gttgctagtc gggcagtgac gacaaatgcg ttgaatattt tcggagatca cggagacgtg
    25321 atggctgcaa gacaaacagg atttgctatg ttagctgaaa gcagtgtaca agaagtgatg
    25381 gatttagcac ctgttgccca tcttgcttct attgaaggag aagtaccttt tttgaatttc
    25441 tttgatggat tccgtacgag tcacgagatt caaaaaatcg aagttttgga atatgaagaa
    25501 ttagctcctc tgatcaatca ggagaaatta gcttcatttc gtaaacgagg gatgaatccc
    25561 aatcatccga ctgttagtgg tacgaaccag aatccagaca tccattttca acaacgggaa
    25621 acgatcaacc cctattatga taaattacct gaaatcgttc aaaaatatat gaaagagatc
    25681 aaccgattac gaggaacttc ttatgacttg gtgacttatt acggagcaga cgatgcggaa
    25741 gaagtcattg tttctatggg atcagttgca cagacgattg aacaaacgat tgattacttg
    25801 aatgcaaacg gacggaaaac aggttttttg aatatccatc tttatcgtcc ttttccagtg
    25861 gaaacattcg tgcaaaagtt gcctcataca gtaaaaacaa ttgctgttct tgatcgaagt
    25921 aaagaaccag gggcaggtgg agaacctctc ttattagacg ttcaaagtgc gttatatgat
    25981 tctgaacttc gcccggctgt tattggtggt cgctacggat tggggtctaa agatgtgact
    26041 cccgaccaaa tctcagcagt ttttgatgaa ttgaaaaaag acccaagcat acgcaaaaaa
    26101 cggtttacca tagggatcgt ggatgatgtg acttatcaat ctttggaacc aaaggaaagt
    26161 ctggatttga cagaaccgca aacattccag gccaagtttt ggggatttgg atctgacgga
    26221 acagttggag caaataaatc agctatcaag attattggag atcacaccga taaatatgcg
    26281 caaggctatt tttattatga ttcgaaaaaa tctggcggtc tgactgtctc tcatcttcgt
    26341 tttggagata cgccgatacg ttcagcgtat cttgtcgagc atgcggacct tgttgcctgt
    26401 cacacaccag cttacctgca tagctatgac ttggtcaaag gattgaagcc aggtggcatt
    26461 tttttattga atactctttg gagcgacgaa caattagaaa cgcatctgcc cttaaaatta
    26521 aaacgttact tggctgaaaa taacattaga ttctatacga tcaatgccat gagattggcc
    26581 caagaagtcg gtttgggacg ccggatcaat acggcaatgg aaacggcttt ttttaaatta
    26641 gctgacatca ttccttttga tgaggtgctt ccgctgctaa aagaagaagc cctgaaaagc
    26701 tatggacata aatcaatggc tatcgtagaa aaaaacatac aagcaatcga tcggacgacc
    26761 gaactgcttc atcaagtcgc gatcccagac cattggagaa cgttggagat gcctgaaaaa
    26821 atcaataaaa ggaacactgg ttatatacaa gaaatacttg aaccagtgaa tgcacaggaa
    26881 ggaaatcaac tctctgttgg tacacttgta gcaaatggaa tgacaactgg cgaaatgcct
    26941 ttaggaacgg cttctatgga aaagcggggc gtcgctttag aagtacctga gtggattagt
    27001 gatcgctgta cgatgtgtaa tgaatgtgcc tttgtttgtc ctcatgctgc catccgtcca
    27061 tttttagcag atgaagagga aatggaagaa gcgccagaag gatttatcgt gagagaaatg
    27121 cgaggcgcag acggcgtaaa ataccgtatc caagtttcag tggaagactg tacaggttgt
    27181 ggtctgtgtg tagaggcttg tcccgcaaaa gaaaaagctt tagtgatgaa gccttatgaa
    27241 gaacagaaag aacaagctgt gaattgggct tttgcaatga cattaagaca aaaagcaaat
    27301 ccagtcaaag gaaagaatac tgtcttgtct acacaattca atcaaccgct tcttgaattc
    27361 tcgggcgctt gctcaggctg tggcgaaaca ccttatgtaa aattattgac acaaatgttc
    27421 ggtgatcgaa tgatgatcgc gaatgcgaca ggctgttctt ctatttgggg cggtgcttct
    27481 cctgcttctc cttatacgac caatgaatgt ggtcaagggc ctgcatggtc aaattcttta
    27541 ttggaagata atgctgaatt tggttatggt atgcttctag cggcacagac tcgtcgcgag
    27601 gctttggcaa gttcaatcaa agaagtattg ccaaaagtat cagaagaact acgagaattg
    27661 atggaagact ggttggtaca catgtttgag agcgaaggaa cgcagcaaag agcagcaaaa
    27721 ctgcaagctg cgatcgaaag cgaacgatca attgtccctg aactggagga tatatacaag
    27781 agaaaggatc tattcgtaaa aaacagccaa tggatgattg gtggagacgg atgggcttac
    27841 gacattggtt ttggcgggat cgatcatgta cttgccagcg gagcagacgt gaatattctt
    27901 gttttggata acgaagtcta ttccaatact ggcggtcaaa cctcaaaagc aacaccagct
    27961 tcggcaatcg ctaaattttc ggctagtgga aagtatgctt caaagaaaga tctaggaatg
    28021 atggcgatga catatggaaa tgtttatgtg gcgcaaattg cttcaggtgc caatcaaatg
    28081 cagacgataa aggcttttga agaagcagaa aagtatcctg gaccttcttt aatcattgcc
    28141 tatacgcctt gtatcacaca tggtttgctt ggtggaatga aagaatcgct taaagaagca
    28201 aaagaagctg tttcttctgg ctattggtca ttatatcgat ataaccctgc aatggtcgaa
    28261 aaaaacaaga acccaatgac tttggactac aaaaaacctg atttctctgc tatgcctgat
    28321 ttcatgagga aacaagttcg cttttcctcg ctagagaacg caaatccaaa tcttgcaaaa
    28381 gctttatttg aaaagactat ccaagacgct aaaacacgtt tttacaacta tgctgcactg
    28441 acgggagaat tagaaaaaat caagaaacga cttgaaccag aggaatctat cgatgtaccc
    28501 gttaagacta gaaaagaaaa aacgacggac cttgaagcag aagaaagaag agctgccaga
    28561 gcagaacgac gtaaaaaaat ggaacaagct acaaaagaaa aaaaggaaaa ataacagaaa
    28621 aaatataatg aataattctc tgaagtttaa aaatatttat tgcagagaaa aataaaacgc
    28681 atacttgcaa ttttcctgaa aattatgcgt ttttctcaag aaaaatacga gcagatcagc
    28741 ctctaaaatt aaaacatatg atatactaga gaggaaatct caaaagagaa gagaggcttt
    28801 ttatgtcatt tcaaatcggg gaacttttta atttggacta ttggcagcaa atgttttccg
    28861 gtgacttgtt ctctctgaat tttttggtga acatacttga tattctggtt gtctggtatc
    28921 ttgtatacaa actgatccaa ctgctgcgag gcacaaaagc gattcagctt ctgaaaggtg
    28981 tagctatttt tattgttatc cgaatcgtag cggaaatcat tggtctgcac accttgtctt
    29041 ggctgatgaa ccaagtcatt acgtacgggg tgatagctgc tatcgtgatt tttcagccgg
    29101 aaatccggag aggactggaa catcttggac gcagttcgct tttcaaacaa tcaaaaagcg
    29161 aacagcagga agatgagaag atgatcttgt cttttgacaa agccatccaa tatatgtcca
    29221 agcgaaaaat cggtgcgcta attactatcg aacggcatac tggattagat gagtatattg
    29281 agacaggaat tgcattagat gcagatatca ctggagagct gctgatcaat atctttattc
    29341 ctaatacacc gctgcatgac ggtgcagtta ttgtaaaaga aggaaagatc gcagtagcaa
    29401 gcgcctatct tcctctatca gaaagtatgt tgattccaaa agaattcggg actcgacacc
    29461 gggcagctgt cggtataagc gaagtcagcg acgcgattac catcgttgtc tctgaagaaa
    29521 ctggggatgt cagcattaca ttagataatg aactgatggc tggcttatcc cagcaagaat
    29581 atttggcaat tttgcgtaga gaactgattg ctgaagagaa aacaaaaaac aagaaaaacg
    29641 tgattcaatc tttcctagac ggtatgacta agggagggga tcgcaaatga tctcaaaaga
    29701 gaaacggaca agcattcttt acagttttat tgcattgctg ttcagtattg cgctgtactt
    29761 caatgcaaat ggccagtccg ttccaaatac gctttctgga aatgaagcgt ataatcaaac
    29821 agttactggg gtgccaatca agatttctta tgattcaaag cgctactata tccatggata
    29881 tgaaaatgca gtgactgtca aactttccag tgcaaaccga gttcagttga atgcagaggc
    29941 aaacgaagat acacggatgt tccgcgtcac agcagatatc actaagctgg gcgaaggaac
    30001 acatgaggtt ccgctaaaaa ttcaaaattt gagcagtgct gtcacagcaa agatcgaacc
    30061 atcgactatt acagtaacgg ttgagaaaaa ggtaataaaa gatttcgatg tggaaacgtt
    30121 gattccttca acgttcacac catctggata tgaacttgat gatacttccg ttagtcctaa
    30181 aaaagtctct atcactacag gtgacaaaac attggcagaa atcaaacgag tagtagctga
    30241 tgtggacgct tcgatggtca cagatgatgg gattaatagt gaggtcccaa tacaggcgct
    30301 gaatgcagca ggggaagtat taagtatcgt ctctgatcca gtgcaagtaa cagtgagagc
    30361 tgattcgatc aagccaagca agtcggtacg actttatggg atacagcaag gaacaccagc
    30421 tgccggggta gaaagctatg atttcagttt ttcaaaactg gaagcagaag taagcggctc
    30481 tagcgatttg cttgcatcga tcggtgacag tattgctgta ccgatcaatg tctcaggtat
    30541 caatcatcga acaactcgta aaattgaaat accagtagaa gaggggttaa gtatcaagcc
    30601 cgaatcagtt agtgtggaga ttacacccgt gttgcaaacg tcaagcagta gctctgcttc
    30661 gacgagcagt cggacacaag aaaatacaag tacgagtaga agtatttcat ctgctgattc
    30721 aggaacggaa ccatcgacaa caaccatttc ttccagttcg gaatctggga caagcagctc
    30781 gaccacaagt acagaagaaa catcagaaac aagcgaatca acacaaatga gcagaacaaa
    30841 ttcctcagaa aattaagtta aaggagtaat tttacaacta tgggtaaata ttttggaaca
    30901 gacggcgtac gcggtgaagc caacaaggaa cttacgccag aacttgcatt taagctggga
    30961 cggtgcggag gttatgtcct aagccagcat gaagaagaag gaaaacgccc acgcgtgtta
    31021 gtagggcggg atacaagaat ctcaggtcaa atgttagaag aagcgctgat ttcaggactt
    31081 ttatcggtgg gtatcgaagt gttccagcta ggtgtgatct ctacaccagg tgttgcttat
    31141 ctgacacgtt tgcaaaaagc aagcgcaggg gtcatgattt ctgcttctca taatccagca
    31201 caagataatg gaatcaaatt tttcggtgca gatggtttta aactagtaga tgaccaagag
    31261 ctagaaattg aagcattatt agatgctgaa gtggatgaat tgcctcgtcc atcagctgaa
    31321 ggattgggaa ctgtagaaga atttccagaa ggcttattga aatattctca attccttgta
    31381 caaacgatca acggtgacct ttccggtttg accatttgtg tcgatgcagc aaacggagca
    31441 acttcaacaa gtgttaatcg tttgtttgcc gatcttgaaa cagattttta taccatgggc
    31501 acaaatccag acgggttaaa tatcaatgat ggcgtaggct ctacccatcc agaacgttta
    31561 gctgagatgg tcgttgaaaa aggagcagat gctggactag ctttcgatgg cgatggcgat
    31621 cgtattattg cggtggatga acttggaaac attgtcgacg gtgataaaat catgtttatc
    31681 tgtgctaaat acttggcaga aaaaaatcga ttgaaaaaag atacgatcgt tacgactgtc
    31741 atgagtaact taggattcca caaagcggtg gaaggaatcg gtttgaaaga tgtcgttaca
    31801 caagttggtg atcgttacgt tgtagaagaa atgcgcaaaa acgattataa ctttggcggc
    31861 gagcagtctg ggcacatgat tttcttagat tttaacacaa ctggagatgg gatgctttca
    31921 ggaattcagt tactgaatat catgaaacag acaggtaaga aattatcaga gcttgcagca
    31981 gaggtaacaa tctatccgca aaaattagtg aatatccgag taagtgataa aaacggtgcg
    32041 atggatgttc cggcaatcaa aaaagtgatt gaagaggcag aagctgaaat gggatcagaa
    32101 ggaagaatcc tagttcgtcc ttccggtaca gaaccattac ttcgagtgat ggcagaagcg
    32161 cctactgatg agaaagttaa ttactatgtc gataaaatcg cggcagtagt caaagaagaa
    32221 atcggactgg attaagagat attttcataa aaaataaaag aacaaggacc agtgtcagat
    32281 gtctgacact ggtccttgtt cttttattat tctactgtca ctgatttcgc taaattacgt
    32341 ggtttgtcaa cgtcgaatcc ccgtaatagc gttgcgtaat atgcaagtaa ttgagtcggt
    32401 acgactgtgg ttagagtagt tagataaggg tggacagttg gtagcaccag ctggtcactt
    32461 tccttttcta atccctctga gacaataact agtgttttag cgccacggct ttcgacttct
    32521 ttcaggtttc ctcgagtatg cgcaccagtc acttcttcac tgatgattcc aatcactggt
    32581 gttccttctt cgatcaaagc aatcgttcca tgtttcaatt cgccagcggc aaaaccttct
    32641 gcctggatat aactgatttc tttcaatttc aaagcagctt caagagaaac gttgtagtca
    32701 gcgccacgtc caatgtaaaa ggcatttctt gtatcgctga gatatacttc tgccaactct
    32761 tctaaataat ctttttgggc gatgaccgat tccatcgcat tagcaactaa actcaattca
    32821 tgagtgatat cgaatacaag agaagtttca aattcttttt tatcaccgat agctttggct
    32881 aataaagtta gtacagctat ttgtgcagta taagctttcg ttgatgctac tgcgatttcg
    32941 ggtccagcat gcaatagcag tgtaaaggat gcttcccgcg aaagagtaga accagcaaca
    33001 tttgtaatcg taagtgaagg gtagccgaga cggttgattt ttaccagaac ttggcggcta
    33061 tctgctgttt cgccactttg actcaagaaa ataaagaatg gtttttttga aagaagaggt
    33121 gtgttatagc caaattcact ggctaaatga acctcaacag ggatctgtgt caatttttcc
    33181 agagtctgtt tacctgctaa ccccgcatgg taactagttc cgcaagcaac gatatagatt
    33241 cgatcgctat cagacaatgt atcgaccagc ttttgatcaa gagagacacg attgtcttct
    33301 gttacgtatt tttgagcgat acgacgcatg ataataggct gttcgtcgat ttctttcagc
    33361 atataaaaag gataggtacc tttctcgata tctgaagaat ctacttgtgc ttcgaatgaa
    33421 gaacgttcta cgatgtcacc tgcaaaattt tgaataataa ctttttctga tgtgacggta
    33481 accatctctt catcttcaat ctcaacgaat tccttcgtat gagcaagcat agccattgca
    33541 tcgctggcaa tcacattgaa tccatcgccg agtccgatta atagtggact tttattttta
    33601 gcgacgtaaa taacatcagg tgctgtccga tcaatcaaag cgaaagcgta agaacctttg
    33661 atgacccgca aagcttttaa aaacgcctct tgcgttgtag tatccttcgc gaaagtttca
    33721 attagatgag cgataatctc agtatcggtc tcaccaataa agtggtcatt tgctaaataa
    33781 gcaattttaa gttcttcaaa gttttcgatt acgccattgt gaacgagcac gaagcgtcca
    33841 ctttgggaag tatgtggatg ggcgttttct tcagaaggtt gcccgtgagt tgcccagcgt
    33901 gtatgaccaa tacctatatt tcctgtagta tcaatattta ttttgttttt caaattttga
    33961 attctgcctt gtgctttgac cagataatca gttgtgccat ctgagacaaa gataccagct
    34021 gaatcataac cacggtattc taatttttct agaccgtcaa tcaatcctgg tgttacattt
    34081 tttaatccaa tcattccaac aattccgcac ataaaaaaca tcctactttc tataatgaat
    34141 tcgtatgaat gcttccagct gcaggaaaat ccttttgttt tatctttgca gatatttttt
    34201 gtggattttc tgtagagtgc agtccctagg gccatccgcc gaatctttcg ataaaccctc
    34261 tcctcgtcat ctgtttacac agacctggcg ctatcctaaa aggtataact atcctcctct
    34321 tttgtttttt atctggaaac atttaaaatc ataacctttt gcgaatgttc ttgtcaacaa
    34381 aaaaagaata aaaaataaat tggtatatat caaatgcttt gtcaaatgct ctttttatgt
    34441 caaaaaataa tgggttttta tctatcatct ttttcaggag ttcggtacaa tagaaagtaa
    34501 tcggatagag gaggagtaaa aatggattca aaaattgaga tcatgacact gggaatgtta
    34561 aaaaaacaat tgtccaaatt cgaagcatca gctggtgtat cagatgatac gaaaatattt
    34621 ttagatactg gctgggatag catccaagaa atcgcacctg atgccttgga agttgctgaa
    34681 gctcgtgagt ttacggtaga agatgaattg acaaaagagt cgttttccgg gtatgcgcgg
    34741 gaagaaaaag cagagcggtt cgacacttct gagcagtcag aaactgtgat cgtcataaaa
    34801 aacttgtatt agaaaggaga aagaatgaat aagagacgtt gggcagcagt tctgattgct
    34861 gcaggattat ttgtggtttc gttagtttca gctagtttga ctaagcctca ggaaaacgat
    34921 caaattgagg gattgaacag ttggttatat ggggatgaag aactagcttc aaatgtgttg
    34981 gaagagggag acagtacgca aaaaatcgta aaattatcag tagatggaac gattgctgcg
    35041 ggagggtctt caggtttatt tgcttctgaa ggttacaatc ataaaaattt tatggaacaa
    35101 ctgaaggcga ttgaagaaga tccaacagtc aaaggtatct ttttagaagt aaattctcct
    35161 ggtggcggtg tctacgaaag tgcggaaatc gctaagaaat tggatacgat tcgtaaagaa
    35221 catgatatcc caatgtatgt cagcatgaaa aacatggcag ctagcggtgg ctactatatt
    35281 tctgctcaag cagataaaat ctttgcaaca gaagaaacag tgaccggctc aattggtgtg
    35341 atcatgtcag gattgaatta ttcaggactt ctagaaaaac tgggtgtgga agatacaaca
    35401 gttaaaagcg gagcgttgaa agatatgggt tcaggaacgc gaccagagac aaaagaagaa
    35461 gaagcggtgc ttcaagcata tatcgacagc gcatatagtc gttttgtagc aatcgtcagc
    35521 gagggacgga ataaatctga agaagaagtc aaaaaaattg ctgatggaag gatttatgat
    35581 ggtgtccaag caaaagaagt aggtttagtc gatgaattgg gatttcctga agatgcttta
    35641 aaagctatgc gaaaggaaca acagcttgaa gatgcggaac tagtagaata ttcttccacg
    35701 ccatctgggt ttgctagtac atggttagga agtaaactag cagaattcca gggattgaag
    35761 ccttctgaga ctagtcagat cttatccgtt cttgagaatc tagggacagc ggaatcacca
    35821 aaagcaatgt attattacgg aggtgagtaa gcatgtcaga gctttcaaat gaaaataagg
    35881 aacaagaaaa agataagcaa cagcagcctg ctgtaggacc tgactccaac ttttcccaaa
    35941 aggtactgag ctcttttgaa agagagcctt tgacgcccga agaaatcaaa aagaaacaac
    36001 gtaaatggaa aaagtatacg gacacaagaa atgaaccgcc aaaagcaaat gattttccta
    36061 agtatttcta tgcaggtttt tttatccgaa tgtttgcttt cttggtcgat cttttgtgta
    36121 tcggagctat tacgcgttta acacttggta ttgccaaaaa tctaggttgg cttaattttt
    36181 cagattctta tctgagtatc tacggattca cagccttact tatttatcta gcgtatttta
    36241 ttttattgac gaaattcaat catggtcaaa cgattgggaa aatgatcttt ggtattcgag
    36301 tcatcagctt tgatgaagaa gaacttagct ggtcaaccgt tttagttaga gaaggtgctt
    36361 gccggttcat tctaaaattt cctttgctga tgttagggta tcttccagca atctttaatc
    36421 agaaaaagca acatctaggc gattttttca ctaatacaag tgttgttacg atcaatctga
    36481 tcaaagcatt caaccaggaa gtcaacagct gatgagcaat aaaaagcagt aacagttgaa
    36541 agcaccttct aaaagtcaga tctaaaatct agcttttaga gggtgcttca gttattttta
    36601 attgtcatgc tttttttgtt ttgctactat tgaacatgag ctgaacatga gcaagaggag
    36661 gacactgcgt gaagccgatt gattttccag aaaaatatga gaatttaatg cgagtagcac
    36721 aacaagcact agcaaaccag caatatcagc aggcaaaaga attatttcaa cgagcttacg
    36781 agttgaaaga aagctttgag gctaatagcc tgcttgtttt ttgtctttac gagttggacg
    36841 agaaaaaaga agcattgaag caagcactct tgcatgaaaa acagtatctt caaaatgagg
    36901 agtttgctga attttatttt gacttgttaa tcagtacaca agatttttta tatgctagaa
    36961 aattgatagc aagtacagat ttttatgaag ctttcgagca gcgggtcatt gaaaaaatcc
    37021 aatttgcaga agaattatcc ggacaaatgg agaggcagaa gatcaaggca ctccacaata
    37081 aatcggagga acttccttcg ttggaacctg cacgtcagct tgcgtctatt gatgagatcg
    37141 aacagcttcc ttatcatgag tttatccaaa cagcgagtaa acttattgta tcgccagagg
    37201 tccatatttt agcaagggca aaattactgg agacacttag gcaattgaac gaatgccatc
    37261 ctgttttcta tttgacgatt gaagaaaagt tagtcaaagt cataccaaaa gatctgccaa
    37321 agccgcagca gcaggcaagt taccagcaat tatgtgtatt ttcagatcgt tacggtaatg
    37381 aagatgcgct gcttagttcc gtgttaaatg aagaattcac gttgcaatct gcaattgttt
    37441 atcctgtcta tgatacatac atcgaagacc cgaagcgctg gtttcaactg actgtagagg
    37501 cgtgtacagg aaaaacgatg tatgaaggta cagaagatga aaaacaagat tttttaaaaa
    37561 aaagagaaaa aattttgcag caaatgatct tcttccatta gattttatca gtgaaaaaga
//
LOCUS       DAXWOO010000008        35797 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.008, whole genome shotgun sequence.
ACCESSION   DAXWOO010000008 DAXWOO010000000
VERSION     DAXWOO010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35797
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.008"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..884
                     /locus_tag="ACGE70_000328"
     CDS             <1..884
                     /locus_tag="ACGE70_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFF8691998.1"
                     /translation="ISVPVALFSFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTY
                     NDYFQIGMIGMIVSLVVVLTVANISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVL
                     GVVLLDLQIILGFILAGIWALLFTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMT
                     LAMFNNSAAYASPYFSAIFGDWIPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILA
                     VVLAVNPTMSPAFLFPLFAITTIAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGW
                     IIGAILCLITFLLYGNA"
     gene            932..3061
                     /locus_tag="ACGE70_000329"
     CDS             932..3061
                     /locus_tag="ACGE70_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFF8691999.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKNLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKAIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPHTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3036..3725
                     /locus_tag="ACGE70_000330"
     CDS             3036..3725
                     /locus_tag="ACGE70_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692000.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEIYEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKEIE
                     KRCTEYDLDYK"
     gene            complement(4133..4819)
                     /locus_tag="ACGE70_000331"
     CDS             complement(4133..4819)
                     /locus_tag="ACGE70_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFF8692001.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(4955..5149)
                     /locus_tag="ACGE70_000332"
     CDS             complement(4955..5149)
                     /locus_tag="ACGE70_000332"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692002.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5199..5639)
                     /locus_tag="ACGE70_000333"
     CDS             complement(5199..5639)
                     /locus_tag="ACGE70_000333"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFF8692003.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELEQLFQAYASLDDVNS"
     gene            complement(5643..6488)
                     /locus_tag="ACGE70_000334"
     CDS             complement(5643..6488)
                     /locus_tag="ACGE70_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFF8692004.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLEKQSKEQWLVIDGSALSLLVANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            6637..8055
                     /gene="pepV"
                     /locus_tag="ACGE70_000335"
     CDS             6637..8055
                     /gene="pepV"
                     /locus_tag="ACGE70_000335"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFF8692005.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(8131..9333)
                     /locus_tag="ACGE70_000336"
     CDS             complement(8131..9333)
                     /locus_tag="ACGE70_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692006.1"
                     /translation="MKLFKKIFFAFVLLIASYCVLLTITFFIPQHSIESNAEKSLQMV
                     EKEGMYPSINQGNMLGTRLDNFTDHLMIRKTKADPELNSLENAMSMADYPRYWHGYQL
                     FLRPLLLGMSLGSIRMIYAAILFLLIGLTSYFLTKCSDIYLTTALLISLAIGNAATFF
                     FSMQFSNIWILTLLSVLLFLTKPQLSNTNQQLFLYFFVIGSLANFFDLLTTPVISWGI
                     PVIIIYYVTNKYLMDKRSSLSKQVGSFVFTGIFWGLGYGLTWVAKWILSSIILNKNIV
                     KDAINQILFRTEGNDKYPLHRLEMLKSNIRLMYPKVAILLLLITCLVFLYFAYRKRNS
                     WRPFQLLVLFVLAAVTPYIWYNILANHSQIHYWFTYRTQIITSFAILSAFAFLIPPEK
                     SKDELNYF"
     gene            complement(9320..11107)
                     /locus_tag="ACGE70_000337"
     CDS             complement(9320..11107)
                     /locus_tag="ACGE70_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6020 family protein"
                     /protein_id="HFF8692007.1"
                     /translation="MHKKKENLFPTFFATLFASGFAFFTSYGLGTLNPTVIIGLAMVL
                     LLAFKQYGIELFSIKNKVAAIARLFFSAAFALTLSIGRQIQFQYVSANYTKTTIADGS
                     IFYFLVYFSIFVYSFLFLQSIYLSMPKLETFFTAQNTQAFSSRKYFLFFTVFLVLCWL
                     PYLLALYPGVVLPDSLSSVAQSMGDVPISNHHPVLFTLLVKLFVHDLPTDTINHGVFL
                     FSIFQTLITASAISYSLCWLLKRNIHFFPVLISLCYFAFAPVFPIYALNMQKDTLFSI
                     ACFLFSLAVFDFIFQREKPTILNQIYVIILALLTTYLRNNGIYVVTGTGLICGLIILK
                     QKRYQAFFQVVLGYTVIAGLLIHPLMARYAIPTETAEQLGVPLQQISRTIVMDGDLSK
                     KDKAFMNQLLPIKDYEAYSPSLADSIKWHKNFNNEFLNTHKKEFLSLWARNLPSNIKI
                     YTDAYILETFGFWVPGTKNSYGFLDTRVNTNNYGMKQVDLIEHLTGLSGMKKIIDARD
                     FFGSGTLLWLAIGSALLAIFKKKYRYLLLFLPGMLVFATIMIATPVAFSLRYVFVLAL
                     GLPVYILSPFLLDKREKMKTDIGDTHETI"
     gene            complement(11120..12073)
                     /locus_tag="ACGE70_000338"
     CDS             complement(11120..12073)
                     /locus_tag="ACGE70_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFF8692008.1"
                     /translation="MESREKIAVLIPCYNEEATISTVIEDFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAAHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVKNTITYLYKTKIRDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMCKDYKPLLFFGIWTIFFFLFGLVMGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYQNKEMK"
     gene            complement(12258..12608)
                     /locus_tag="ACGE70_000339"
     CDS             complement(12258..12608)
                     /locus_tag="ACGE70_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFF8692009.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKTSFLKEGAIEGAWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLDISRKKGAID"
     gene            12808..13887
                     /gene="pepA"
                     /locus_tag="ACGE70_000340"
     CDS             12808..13887
                     /gene="pepA"
                     /locus_tag="ACGE70_000340"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFF8692010.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            14030..14350
                     /locus_tag="ACGE70_000341"
     CDS             14030..14350
                     /locus_tag="ACGE70_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFF8692011.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VIKG"
     gene            14372..14836
                     /locus_tag="ACGE70_000342"
     CDS             14372..14836
                     /locus_tag="ACGE70_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFF8692012.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKALEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKQMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYAIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            15032..15637
                     /gene="ytpR"
                     /locus_tag="ACGE70_000343"
     CDS             15032..15637
                     /gene="ytpR"
                     /locus_tag="ACGE70_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFF8692013.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVASYGMICSARELHLPNAPEKKGILVLPEDAVVGEVFPK"
     gene            complement(15676..16965)
                     /locus_tag="ACGE70_000344"
     CDS             complement(15676..16965)
                     /locus_tag="ACGE70_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFF8692014.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVMMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            17393..17872
                     /gene="rlmH"
                     /locus_tag="ACGE70_000345"
     CDS             17393..17872
                     /gene="rlmH"
                     /locus_tag="ACGE70_000345"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFF8692015.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            18225..18539
                     /locus_tag="ACGE70_000346"
     CDS             18225..18539
                     /locus_tag="ACGE70_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFF8692016.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            18555..18941
                     /locus_tag="ACGE70_000347"
     CDS             18555..18941
                     /locus_tag="ACGE70_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFF8692017.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            18970..20355
                     /locus_tag="ACGE70_000348"
     CDS             18970..20355
                     /locus_tag="ACGE70_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFF8692018.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            20358..20511
                     /locus_tag="ACGE70_000349"
                     /pseudo
     CDS             20358..20511
                     /locus_tag="ACGE70_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            20534..21739
                     /gene="mobT"
                     /locus_tag="ACGE70_000350"
     CDS             20534..21739
                     /gene="mobT"
                     /locus_tag="ACGE70_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFF8692019.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            22523..24433
                     /gene="ltrA"
                     /locus_tag="ACGE70_000351"
     CDS             22523..24433
                     /gene="ltrA"
                     /locus_tag="ACGE70_000351"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFF8692020.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKRNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            24537..24761
                     /locus_tag="ACGE70_000352"
     CDS             24537..24761
                     /locus_tag="ACGE70_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692021.1"
                     /translation="MSGTYLSIYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFL
                     IIAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            24878..25375
                     /locus_tag="ACGE70_000353"
     CDS             24878..25375
                     /locus_tag="ACGE70_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFF8692022.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSISELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMSDVARYYIEETGALGEVPASLQNYIDYESYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            25462..25854
                     /locus_tag="ACGE70_000354"
     CDS             25462..25854
                     /locus_tag="ACGE70_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFF8692023.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAV
                     MILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRRKLTY
                     AGKKVTLGRNQPQEAITAVRSEFYGISN"
     gene            25838..28285
                     /locus_tag="ACGE70_000355"
     CDS             25838..28285
                     /locus_tag="ACGE70_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFF8692024.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSILSAQERSKNEVTGKLKTVAYDKIDQQTDALISM
                     IGENQVDYRFFIGFKLLLNDQEFSMKSLTVEAKNAFTDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYPLSKKKLEH
                     ETLIKTYDLIKPTRCLVEEKQRYLKIQQEDETAYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVNLTSDEKNKGLLDPYVIMKNTKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSDGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            28288..30471
                     /locus_tag="ACGE70_000356"
     CDS             28288..30471
                     /locus_tag="ACGE70_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFF8692025.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            30468..31469
                     /locus_tag="ACGE70_000357"
     CDS             30468..31469
                     /locus_tag="ACGE70_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFF8692026.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            31484..32398
                     /locus_tag="ACGE70_000358"
     CDS             31484..32398
                     /locus_tag="ACGE70_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFF8692027.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            32712..32759
                     /locus_tag="ACGE70_000359"
                     /pseudo
     CDS             32712..32759
                     /locus_tag="ACGE70_000359"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            32775..34694
                     /gene="tet(M)"
                     /locus_tag="ACGE70_000360"
     CDS             32775..34694
                     /gene="tet(M)"
                     /locus_tag="ACGE70_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFF8692028.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            34792..>35797
                     /gene="tet"
                     /locus_tag="ACGE70_000361"
     CDS             34792..>35797
                     /gene="tet"
                     /locus_tag="ACGE70_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tet(L)/Tet(K)/Tet(45) family tetracycline efflux
                     MFS transporter"
                     /protein_id="HFF8692029.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIKKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     S"
BASE COUNT        11676 a         6492 c         7334 g        10295 t
ORIGIN      
        1 gtatttcagt accggttgca ctgttctcat ttatgggctc gattatggct ggaatctttg
       61 ctaatatcgt caatttcaaa caatatcaaa cgatttatgc tggtttcaat ccagcggctg
      121 aaagctatac ttataacgac tatttccaaa ttggtatgat tgggatgatt gtaagcttag
      181 ttgtggtact aacggtcgct aatatatcaa tgaacaaaaa gaaacgctat gcaatggcag
      241 caaacgtacc agctgaaggt ggcgacgcac caatgatctc atggttagcc gtattattac
      301 cagttttagg ggtcgttctt ttagatcttc aaatcatctt aggttttatt ctagcaggta
      361 tctgggcatt gctgtttact ggaaaattgc gcggaggtta taaagaaatc tgtcgtcagt
      421 ttgcgaaatt atttacagat ggcgcagtcg atgttgcacc aatggttggt ttcttgatga
      481 ccttagcgat gttcaataat tcagctgcgt atgcttctcc ttatttttca gctatttttg
      541 gagattggat accgcaatca ccattagtct tggcaatcgt atttgctata ttgacaccac
      601 taggattttt ccgcggtcca atgaatctag tcggttcagg ctcagctatt ttagctgtcg
      661 tgttagctgt taatccaacg atgtcgccag cctttttgtt cccgttattt gcaattacaa
      721 cgattgctcc tcagcattta gatattacac aatcttgggt tgcttgggga ttaggttata
      781 caaaagtaac ttctcgagaa tatatgaaga aatccatccc aactggttgg atcatcggtg
      841 cgatcttatg cctgatcacg ttcttattgt acgggaacgc ctagtaaaca aaacgtcgat
      901 aaaaacgatt agataaatgg aggaaaagaa aatgaaacgt tttgtgcgaa tgggaattga
      961 tgttggcggt acacatacga aagcagtcgc aatcgataat gcgacgcacg aaatcatcgg
     1021 aaaatcttca gtgaaaacaa cccacgatga tgtccgtgga gtagccgcag gtgtcgttca
     1081 gtcttttcaa aattgtttgc gggaaaataa tatcagtcca gaagatgttg tgtttgttgc
     1141 acattctacc acacaagcga ctaatgcact gattgaagga gatgtagcaa aagtcggcgt
     1201 aatcgggatg gcaaaaggtg gattagaagg ttttttagca aaacgacaaa ctagattgaa
     1261 cgatatcgat ttagggaata agaaaaaaat agagattgtc aatgcattct tacctgtcaa
     1321 aaacttaaat gtagaccgtg tttcagaaac gatttcttct ttagaaagag aaagagcgga
     1381 ggtcttggtt tcttctatgg catttggagt agataatggg gaaccagaac gagttgttta
     1441 tgaagcagca tctgtgaaag cgatccctac aacgatggct tctgacatca caaaattata
     1501 tggattgaca agaagaactc ggacagcagc aatcaacgca tctattttac caaaaatgct
     1561 agatacggca acctcgacag aagactccgt acgagaagca ggggtcaatg tttctttgat
     1621 gattatgcgc ggagacggcg gagtaatgga aatcaatgaa atgaaaaaac gacctgtttt
     1681 aaccatgctt tccggtcctg ctgcatcagt catgggctct ttgatgtact tgagagcgtc
     1741 taatggtgtt tattttgaag taggcggaac aacaaccaat atcggggtca ttaaaaatgg
     1801 acgtccagca atcgattatt caattgtagg tggccaccca acatatatct cttcattaga
     1861 tgttcgggtt ctaggtgtgg caggcggttc gatggtacgg gctaatcagt caggaatcat
     1921 cgatgtcggt ccacgttctg cacatattgc cggtctagat tatgcagtat ttacggagac
     1981 agaaaaaatc aaaggtccta aggttgaatt tttctctcct aaagaaggag accctgctga
     2041 ttacgtcaag gtagtgatgg aagacggaga agaagtgact attacaaata catgtgcagc
     2101 caatgttctt ggattagtgc aagaagaaca tttttcttat ggaaatgtcc catctgcacg
     2161 aaaagccata caggcattag cagactattg tcatacgacg gttgaagaca tcgcagagca
     2221 gatcatggaa aaatcctatg ctaagattga accggtgatt ttagaattgg cagacaagta
     2281 tcacttggag aaagatcaga tttctcttgt aggtgtaggg ggcggcgctg cctcattgat
     2341 tacttacttt agtaataaaa tgggtgtgaa atattcgatt cctgaaaacg ccgaagtgat
     2401 ttcttctatt ggtgtagcac ttgcaatggt ccgagatgtt gtagaacgta tcatcccttc
     2461 gccaagtaaa gaagatatac gatcgttgaa aaatgaagcg atgaataaag cgattgaatc
     2521 aggcgctaca ccagaatcga tcgaagtcca cgtagaaatc gatccacata cttcaaaagt
     2581 aacggctatc gctactggtt ctacggaagt gaaagcaacc gatctaacca aggagatcac
     2641 aacagaagaa gcactagagc ttgctgcaga ggatatgcga ttaaataaaa atgaagtttg
     2701 cttattggaa aatacacctt tcttttatgt atgcggcgaa cagaatcgtt caaaaaatgc
     2761 tggaagtctt cggatcatcg atcaaaaagg atttatcaag gtccaacgtg gacatgcttc
     2821 atgtatgaaa acaactgcag ccaattatat gacagctgtc gaacaacttt gggaagatat
     2881 ggcagtttat caaactgaat tgattgcccg accagaattt tatctctgct taggagcaag
     2941 agtctctgat tttacagcga cggatctgga acaactacag cttttgatgg atctggaagt
     3001 atctactatg gaaccggagg aagaagtgat cgtcgttgcc ggaaatatca aacaaacata
     3061 agttagaaga gttactaaac gaattacaac agatccccga agaaatttgg ggattctacc
     3121 agtttcggcg agatttattt tggaaaaaga ttcctttgtc caagcagaaa attcttatac
     3181 aacaatcaat cgattgtggt atcgaaacgg catgtagcat caaaaaaaag tacccttttg
     3241 ccgatgtagg agaaatctat gaacaaatgg ctgttccaat cgtttcctgt gaatcagagc
     3301 aaataaacga acgaatcact tttgcaactt atgcagaaga tgaagggatc cgattgatga
     3361 cggagccgtt agaaaaatta aaatgttcag gactagcttc gatctctaaa gaaacagcgc
     3421 aagctttgat tattggacat gaactatttc accacatcga agcttctgtt aagggaattt
     3481 atacgcaaag cgaaaaaatt gtgctgtggc ggttgccttt ttatacacat caatcaaaca
     3541 ttcgtgcatt gagcgagata gcagcgatga gtttttcaaa agagatgaat caaagcgatt
     3601 tttcgcctta tgtattagag gcagtcttac tttggccata taatgaaatt cgtagccaag
     3661 ggatcttaga agaaataaaa gaaatagaaa aaagatgtac ggagtatgat ttagattaca
     3721 aataaagaga ctgtgacaaa agtcgtgtca cagtcctttt tttccgaata aacggtgttc
     3781 tcctgtcaac tcctcggtca caagtcaaaa tattcaacaa acatgagaag ctgttttttg
     3841 aaattgttcc ttgtgactcg gagatgaacg gcaggctcac gacctcttgg aaaaaggtgt
     3901 tcaaaagctt gcatctgcgg taataagaac agccaagtta aaaacataag aagctatttt
     3961 tacttggctg ttcttattac ttgatgcagg gcagcttttt cacaacctct caataaacgg
     4021 tgtgcaatca gctgaccctc ggtattagct caaaaaaaac ggcaaaatat gaggaactat
     4081 tttgccgttt atttctctaa tactcaggtc aaaacgctga ttccaaaacc tcttatttga
     4141 tgggtctgtc ctgaatatct tcaaaataat cggggtaatt atgaatcacg tattcttgct
     4201 cctcgataat catatgtaaa tgcatcgtgc aaagaaatcc gacttcatca aagttatgat
     4261 caatcattga ttgaagcatt gtctgatgtt catctaatac tcgaccccag tctaattctg
     4321 aaatcgtcaa acgtaaccat ctgaaacggt tcaagtgcgt actataactt tctagccaat
     4381 cccaaatttc tcgttttcca gcaatttcat aacaagttcg gtgaaaatca gtatccaaat
     4441 caaaaaaagc ttttttattt tgagcgatca ctgcctgatc ggtttgttct agtatttttt
     4501 ctagtttagc ttgatcttgc ttggtcattt tcgcactgca ttcttgcagg atcggttttt
     4561 ccaacttttc ccgcgtataa cgggctaatg aagcagatcg gatatcgatt tttgaaacat
     4621 aagtgccgga ctgtggaatg gtttttacaa gctcttgatt gcgcagttgg atcagtgctt
     4681 ctcgaatcgg tgtacgcccg atagctagtt gttcttctaa cactttatcg gagattttac
     4741 ttccaggtgc taactcggaa taaatgattt gtttacgtat agattcataa gcttgctgtt
     4801 gcaaattatt gataaccatt taaccttcct ttctatgcag atataatcta ttataacata
     4861 aatcgcttac aaatcgtgtg ggcataaaaa cacagtctgc aaaaaaagcc ctgataggca
     4921 tgcctattag agcctcgaaa agaaaaatat tgaatcaatc tggtcgtacc tcttggatcg
     4981 gtttgacacg accgccgatt cttttagcaa aagcttcgga ttcttttttg tctttgaaaa
     5041 tgagcagatt gtctaagttt ttttcccgag tgcgacagcc agttttatcg atgtaaccgt
     5101 gcattacttt aacgacatac atggggatca cctgcttttt tcttttcatt atacgaacta
     5161 ctcgacacaa tacaagaaaa atgcttagac ggtatttatt aagaatttac atcatccaaa
     5221 gaagcgtacg cttggaataa ctgttctaac tccggattga ctttgctcag cttcactaat
     5281 cgttgcgaat cagaaagtaa aaaacagtgt ttactactgc cagtagtttt taattttttg
     5341 tcttcagttc ccacaatttc gtagctaaaa tccaaccgtg ttcccgtata cttttggata
     5401 gttgggtgga tgctcacttt ttctccgtaa tggatcattt ctttatagct gcaatttact
     5461 tccagaacgg ggatgatgat cccagattct tcgatttttt tatagggaaa ccctagatgt
     5521 accaataagg ctacccgagc ctcttcaaac cagcggatat aattgctgtg atgaataatc
     5581 cccatttgat ctgtttcata ataaaaaggt tctcgcacat aacctgtaaa tttctccatg
     5641 actcatgcct cgttttccat tgatttttta caaaaataat tcatccactt cggaagtgct
     5701 tcgctgattt gtgtcggcaa aacaatatat tgttcttttg ccagctggtc tccaatgaaa
     5761 ctatgaagat aaacagctgc tgcaatcgtt tggtcatttt gaggaaactg ggctaaaaat
     5821 cctgtgatca tcccagctaa agtgtcaccc atgccgcctg ttgccatagc agcagaacct
     5881 aaaggattgg tgtagaagtg ctggtgacca taaatttttg tctcatgact tttcaaaacg
     5941 agcacagcac cgatttcctg ttgcttcttt ttgttttctt tatcccgttg ttcttgtatc
     6001 ggtagagaac tcaaccgttg ccactccatt tgatgaggtg taagtacagt ttgttcggga
     6061 tagggtagca aaagatgatt agcaacaagt aaacttaaag cagagccatc aatcaccagc
     6121 cattgttctt ttgattgttt ttctagtact tgtttgaaac gttccaaact cttagaagaa
     6181 gtacccaatc cgggaccaat caagagtaca tctgcttgct ttattacgga aagaaactgg
     6241 tcagtatccg tccaatctac aatcattgct tctggcagat gactgtgaag agcactccga
     6301 tttttttcat cagttactac agtcgtgaga cccgctccac tgtgtacggc agctaaagca
     6361 ctcataatga ttgctccgcc atattgttca ttgccgccaa tcaaaacgat acgtccaaat
     6421 gttcctttat gtgaaatctt tggacgaggt gtgatgactc ttttcactag ttgttcgttt
     6481 atcagcatcg aaaaacctcc ttttactcat tatagcgtat atttttttta gagaaaaaca
     6541 gataagaaaa ggaattgtca gattttttga actatttttt tctgtaattt gtggtatcat
     6601 gaacacgaag acttaaataa gtggaggatt aactttatga caattgattg gcaaaaagaa
     6661 gtagaagcac gtaaagaaga cttgcttgaa gacttgaaaa atcttttacg tgtcaacagc
     6721 gagcgcgatg attcaaaagt aacaccagat gcaccatttg gacctggacc tcgcgacgca
     6781 ttgaaacata tgttagctta tggcgaacgt gatggattta aagtaaaaaa tgtcgataac
     6841 tatgcagggc atattgatct tggcgaagga gacgaaacgc tagggatatt tggacacatg
     6901 gatgtcgttc ctgcaggaga tggctgggac acagatcctt atgaaccagt aatcaaggat
     6961 ggaaagatct ttgcacgcgg atcaagcgat gacaaaggtc caagtatggc agcttactat
     7021 gcgatgaaga tcattaaaga acttgacttg aaactatcaa aaaaagttcg cttcgtagtc
     7081 ggcagcgatg aagaaagcgg ctggggcgat atggcttatt atttcgaaca tgaagaagaa
     7141 ccagattttg gtttctcacc agatgctgaa ttcccaatca tcaatggtga aaaaggaaat
     7201 gtttctttag ctttacgctt caaaggcgac aatgcaggag attatgtctt gaaatctttt
     7261 gtttccggac ttcgcgaaaa tatggtgcca ggaacagcaa ctgcagcact tcaagtacca
     7321 agtgcagatg cagctatcgc gatggaagaa gcattttacc aattcatcga agcaaatcca
     7381 gtaagcggaa cgattgaagc ggacaatacg tatgtaaaaa tcgaattagt tggtaaaggc
     7441 gcacatggtg caagcccaca atctggtatc aatgcaggtt ctttcttggc attgttctta
     7501 gataactatg aattcctagg atctgctaaa caatttatcc atgtagcagc tgcttatgtc
     7561 catgaagatt tttatggaga aaaactagga gtcgcttatg aagatgaaaa aatgggcaaa
     7621 ttgacaatga atgctggact atttgctttt gaagaaaatg gtacagagga agcgaacttc
     7681 atcaatatga atttccgttt tccaaaaggt gtaacagtag acggattgca aagtgacatc
     7741 gaacaaacag tggggcaaga aggcgcaaca gttactcgag gtgctcgcgt aatggaacca
     7801 cattacgtac caatggatga tccattggtg gctacattat tgcaagtcta tgaagaccac
     7861 actggagaaa aaggctacga acaaatcatc ggtggcggaa cttacggacg tttgttgaaa
     7921 cgtggcgttg cttatggagc gatgttccca ggatatactg atacaatgca tcaagcaaac
     7981 gaatttatga gtcttgatga cttgttcaga gcaacagcaa tctatgcaga tgccatctat
     8041 cgtttggcga aataaaaata ctaaaaaact ccggctatct ctgacagctg gagtttttat
     8101 gtttaaaaat gaattagtac atcggtattc ttaaaagtaa tttagttcgt cttttgattt
     8161 ctcaggaggg atcaaaaagg cgaaagcact aagtatagca aaacttgtga tgatttgcgt
     8221 acgatacgta aaccaataat gaatctgtga gtgattagct aaaatattgt accaaatata
     8281 cggcgttaca gcagctaaaa caaacaaaac tagaagttgg aacggacgcc agctatttcg
     8341 tttacgataa gcaaaataga gaaaaacaag acaagtaatc aatagcaata atattgctac
     8401 ttttggatac atcaatctaa tattgctttt cagcatctct aatcgatgca acgggtattt
     8461 gtcgtttcct tccgttcgaa agaggatttg gttgattgca tcttttacaa tgtttttgtt
     8521 caatatgatc gatgatagga tccatttagc cacccatgtc aaaccatagc ctaatcccca
     8581 aaagataccg gtaaaaacaa aactgcctac ttgtttgctc aatgaactgc gtttatccat
     8641 taaatactta tttgttacat aataaataat gatcacaggt atcccccaag agatgaccgg
     8701 tgttgtcaac aagtcaaaaa agttagctaa agaaccaatg acaaagaaat acaagaataa
     8761 ttgttggttg gtgttggaga gttgtggttt tgttaaaaat agtaatacag acaataaagt
     8821 caaaatccaa atatttgaaa attgcatact gaagaaaaag gttgcagcat ttcctatagc
     8881 aagcgaaatc aacaaggctg tcgttaaata gatgtcgcta cacttggtta aaaagtagct
     8941 cgtcaatcca atcagcagaa aaagaatggc tgcgtaaatc attcgaatac tccccaaact
     9001 catccctagc agtaaaggtc gtaaaaaaag ctgataacca tgccaataac ggggataatc
     9061 cgccatagac atcgcgttct ccaaggagtt caattcagga tctgcttttg tttttcgtat
     9121 catcaaatga tcagtaaaat tatctaaccg agtgcctagc atatttcctt gattgataga
     9181 aggatacatt ccttcttttt caaccatctg tagagatttt tctgcattcg attcaataga
     9241 gtgctgcgga atgaaaaaag taattgtcag tagtacgcaa tagctagcga tcaagagaac
     9301 aaaagcaaaa aatatctttt taaatagttt catgagtatc tcctatatct gttttcattt
     9361 tttctctttt atctagtaaa aaaggagaga ggatataaac aggtagacct aatgctaaaa
     9421 caaacacata ccgtaaacta aaagccacag gagtagcaat cataatagta gcaaaaacaa
     9481 gcataccggg aagaaaaagg agtaaatacc gatatttttt cttaaagatt gctaaaagtg
     9541 cactgccgat agctaaccac agtaatgtgc cggaaccaaa gaaatcacga gcatcgatga
     9601 tttttttcat tcctgacaat ccagtaagat gttcaatcaa atctacttgc ttcataccat
     9661 aattattcgt gtttacacga gtatctaaaa agccgtaact attttttgta ccaggtaccc
     9721 agaacccgaa cgtttctaaa atataagcat ctgtatagat ttttatatta cttggtaaat
     9781 ttcttgccca tagtgataga aattcttttt tatgtgtatt caaaaactca ttgttaaaat
     9841 ttttatgcca cttgattgag tctgcaagac taggagaata cgcttcataa tcttttatcg
     9901 ggagtagctg attcatgaat gctttatctt tcttagacaa gtcaccatcc ataacaatag
     9961 ttctagaaat ttgctgcaaa ggaacaccta attgttcagc agtttctgta ggaatagcat
    10021 aacgagccat caacgggtgg atgagtagtc cagcaatcac tgtataaccc agcaccactt
    10081 gaaagaatgc ttgatacctt ttttgtttca aaataatcaa gccgcaaatc aaacctgtgc
    10141 cggttactac ataaatccca ttatttctta gataggtggt cagtaaagca aggatgatta
    10201 cgtaaatctg attgagaatc gttggctttt ctcgttgaaa aataaagtca aaaaccgcta
    10261 aggaaaataa aaagcaagcg atagagaaca aagtatcttt ttgcatgttc aaggcgtaga
    10321 ttggaaaaac cggtgcaaat gcaaaatagc ataaactaat gagtaccgga aaaaaatgga
    10381 tgtttctttt taacagccag caaagagaat aacttatcgc tgaagctgtt atcaatgttt
    10441 gaaaaatcga aaataaaaat acaccgtgat tgatcgtatc agttggtaaa tcatgaacaa
    10501 atagcttgac cagtaaggtg aataagactg gatgatgatt gcttatagga acatcaccca
    10561 tagattgggc gaccgaactt aacgaatcag gcaaaaccac tccaggatac aatgccaaca
    10621 gataaggaag ccaacacaga actaaaaaga cagtaaagaa taaaaaatat tttcttgaag
    10681 agaaagcctg tgtgttttga gcagtaaaaa atgtttccag ttttggcatt gataaataaa
    10741 tcgattgtaa aaataaaaaa gaatacacaa aaatggagaa ataaacaaga aagtaaaaaa
    10801 tacttccatc agcaatcgtt gtctttgtat agtttgctga gacatactga aattgtatct
    10861 gtctgccaat agaaagtgta agagcaaaag ctgcagaaaa aaacaatcgg gcaatagctg
    10921 caactttgtt ttttattgaa aacaactcta ttccatattg cttaaaagca agtagcaaaa
    10981 ccatggctaa accaatgatt actgttggat tcaaagttcc tagaccataa gaagtaaaaa
    11041 aagcaaaacc tgacgcaaat aaagttgcaa aaaaagtagg aaatagattc tcctttttct
    11101 tatgcataaa aaaggcctcc tacttcattt ctttattttg atattcgtgg tagacatggt
    11161 ataaattcaa ttcatattct ttcttggcat ttgtgacgac cgtatccaaa atcaaccctg
    11221 ttactaatga aagaagggct aaagtcatca atccagtact taaaatcgca gaagggatgc
    11281 gtgtaataaa gcttgtcagc atatattcac gaatcacagg aatccccatg accaatccaa
    11341 ataaaaagaa gaagatggtc cagatcccaa agaaaagcaa aggtttataa tctttgcaca
    11401 ttttgatgat catcatgatc actttgaatc cgtcagagaa tgtattcaat tttgattcgc
    11461 taccttcagg acgatcgcga taatcgattg gaatctcaac gaacttgaat tttttatcta
    11521 aagcatgaat cgtcaattct gtttcgattt gaaaaccaga actcataata gggaagcttt
    11581 tgacaaaaat tcgattgaat cctcgatagc cggtcatgac atcgcgaatt tttgttttat
    11641 ataagtaagt aattgtattt ttaaccagat tattcccaaa atcatggaaa ggacgcttat
    11701 tttcatcaaa ataggttcca tttgatagac gatcgccaat gaccatatct gcttcgccag
    11761 aacgtaattt ctcaagcaaa ccatgtgcag cttctgcagg ataagtatca tcaccatcta
    11821 ccatcaaata ataatcggca tcaatatcaa aaaacatttg acggattaca ttcccttttc
    11881 cttgacgtgg ttctttcttt acgattgctc cgccttgaac ggccaattcg tatgttcgat
    11941 cggtcgaatt attatcatag acataaatat ctgcttctgg taattccctt ttaaaatctt
    12001 caataacagt tgaaattgtc gcctcttcgt tataacaagg aatgagcacg gcaatttttt
    12061 cacgtgattc catttgtcac tctccttata taataaaact aatcttattt caataatacc
    12121 gtataataac tacaaatact agctattcca agaggaaata gccctatttt tataaaaatc
    12181 acccactata aaagaaggat aggatacctc tcttttagat agtgggtgcg tattcgttat
    12241 ttcatttttt tgacgtatta atctattgcg cccttttttc ttgaaatgtc taatacggtt
    12301 cctgtatgag catctgctaa aaattcataa taaacagttt cgccatcttc tatccgggaa
    12361 agcccacccc gataagcact cgaccgaacg gcgaatttcc gaataggttc tttttcaaaa
    12421 gagatccaag caccttcgat tgcgccttct tttaaaaaag atgttttgat attttcaagc
    12481 acttggtctg ctgataacac ttttttgcga tgataccaaa tcgttgaagc taccccactc
    12541 aataatccta gaccgatccc aacggctaac ccgcctttaa aatagcttaa ttcgctttct
    12601 tcatacatgt ggatatacct ccttcttctc acttgctact ctttgtgttt attttaccac
    12661 actctcatat tttctttgaa ataaataacc gctaccagaa aaaataagct ataataaata
    12721 cgatcggtct gcagaaaaaa tcatatttta tccgcagatc aatataagtc tgagaggagt
    12781 ttagtctgaa atttagacga atagcatatg gaagaaaaaa catttcaacg aatcaaagag
    12841 ttaacagaat tacaaggaac aagcggtttt gaacaagacg ttcgcgccta tatggaaagt
    12901 catatggaac cgctggtaga tgagttacag ttagatggtt tgggcggcat ttttgggctg
    12961 cgccaccatg aagaagcgga tgcacctcgt gtaatggtcg cagcacatat ggatgaagtc
    13021 ggattcatgc tgacacaaat ccaagaaaac gggcttttta aagtcgtacc tctaggcgga
    13081 tggaaccctt acgttgtttc tgctcaacgt tttacactta aaacctctac tggtaagaat
    13141 tatccatgta tttcttcttc cgttccccca catttattac gaggaacaag cggacaaaaa
    13201 tcagtagaag taacagatat tttgttcgat gctgggtttg aatcaagaga agaggcaatg
    13261 agttacggtg ttcttccagg agacacaatc gttccttacg ctgaaacgat caaaacagcc
    13321 aacgggaaga atatcatcag caaatcgtgg gacaaccgct atggctgtac aatggtatta
    13381 gaagctttag aagcgttaca aaatgaaaca ttaggtcata cattgattgc aggcgcgaat
    13441 gtccaagaag aagtaggcct gcgtggttct aaagcttctg tcaataaatt caagccagat
    13501 ctgttctttg cagtagactg ctctgcagca gatgacacag tgacgaaaaa tggcacattc
    13561 ggacatttag gagaaggtac attgatgcgt atccaagatc caggtttgat catgcttcct
    13621 cgtttgcgtg agtacttgct agatatcgct gaaacaaaca acatccctta tcaatacttt
    13681 gtttcaaaag gcggtacaga tgcaggtgct gctcatacac aaaatgaagg aatcccaagt
    13741 acagtgattg gcgtagtggg acgttatatc catacgcatc aaacgatgtt cagtattcgc
    13801 gattttgaag ctgcacgaga aatgttgatt caaacattga aaggtttaga taaatcaaca
    13861 gtgaacacga tcgtatacgg gaaataacgc gatagtgaaa ctataaaatc tggcaaaagc
    13921 aaccttttgt cagatttttt ttataaaaag aggatcaatg tttgttagtt cgaaagaaag
    13981 gtgtataatt taattgaaaa atacataaac agaaagtaga ggaaaaagaa tgattatccc
    14041 aaatagttta gaagaattag caggctacgt agaaactgga aaaagtgtat ttttctttac
    14101 tgccggctgg tgcggagact gccgttttat caaaccgcaa atgcctgaaa tcgaaaatga
    14161 cttttctgat tggcaattta ttgaagtaga ccgtgacaag tacatagatg tagccgctga
    14221 atggaacatt tttggtatcc caagttttgt cgtgatccaa gacggaaaag aattaggtcg
    14281 tctagtgaat aaagatcgta aaacaaaaca ggaaatcgaa tcgttcctag aaagtgtaat
    14341 aaaaggctga catctgttag gaggggtcaa gatgaatcaa gaatataaga acattttagt
    14401 aggtattgac ggtagtgaac aagcacttgc tgctttcaaa aaagcgttag aagtggctcg
    14461 ccgtaataat gggaaagtat atgtagcaaa cgtaatcgat cagcaatttt ataattttat
    14521 gggctatgct cctattgatc aaaatctagt cgatcagcaa actgaaacag ccaagcaaat
    14581 gatcgaagaa tgtaaagaat atggaaaatc tgttgattac acaaatatag aaggaattat
    14641 tgcatacggc tctgttcgtg aatctatggc agtcaaattg ccaaaaaaat atgcaatcga
    14701 tctaatcatg gttgggcagt ctggactaaa tgcagtcgaa cgctttatga caggaagtgt
    14761 agccagctac gtcatacgtc attcaccttg tgatgtcttg atcgtttctg acgaagaaga
    14821 acagccgagt aattaagcaa gtggaacttg tataaccttt ttaatcaaac agtaaaggga
    14881 ctgtgacaaa agtcttgtca cagtcccttg ttacgaataa atggtgttcc agaagaagct
    14941 tcttcggaaa taaagcatga aaaaaatctc atgcagtgaa tgcaattttt tgttaagata
    15001 aatctcagca aaggataatg gaggaaagaa catgattttt gcttataaca aagcacacgt
    15061 cggagacact ttgatggtta tcatagccga cgataaagga acagaaaaca ctgtcgaacg
    15121 taaatggaat gttgctcgga tcaaagatga acaaggtcaa gttgttgctt ggaacttttt
    15181 ccatatctcc gatcatctaa caattgaagg gaacggccaa gtaagcttaa cagatgaaga
    15241 attagcgaaa ttgaatcgat tgattaaaga agcaggattc gaagaacagc ttgaagctga
    15301 tcatgaaccg aagatcgttg tcgggtatgt aaaaacatgc gtcccgcatc ctgactctga
    15361 ccatttatcg attactgaga cagaagtgga caatgggaca gtattgcaaa ttgtttgcgg
    15421 tgcaccaaat atcgaagctg gacaaaaagt tgtcgttgca aaaccaggag ccatgatgcc
    15481 agatggaatg atgatctggc caggaacttt gcgaggggta gccagctatg gaatgatttg
    15541 ttctgctcgt gaattgcatt tgccaaatgc tccagaaaaa aaaggaatct tagtattgcc
    15601 tgaggatgcc gtagtcggag aagtatttcc aaaataaaga ggttgtgaca agtctttgtc
    15661 acaacctctt ttttattaat tttcactatt ttgattttgt tttaatgccg attgatcgat
    15721 cgtcaattca acagtagttg ttttttcttt cgagccttca tagtaagtga ttttgacaga
    15781 atctcccact tttttcttat acaaagcaga ttgaagttct acaccagaag aaacttcttt
    15841 gtcatcgatc ttagtaatga cgtcgtattg tttcaaccca gctttttctg ccggtgtagc
    15901 tgtttggaca tttgtcacga tgacaccgtt agttacagat gaaggaatct tcaagatttg
    15961 ttcttgttgc tgagtagaaa cggcagataa gtcgaccatt gtgataccta gagctggacg
    16021 tgtcactttt ccgtcttttt ccagttggtt gatgatattc actacatcgt tacttgggat
    16081 ggcaaatccc attccttcta cgcttacatt agaagtcgat tcagaagtac ttgcgatctt
    16141 acttgagttg ataccgataa cttgtccttc gatattcact aaaggaccac cagaatttcc
    16201 cgggttgatc gctgcatctg tttggatcgc attgatattg actgtttcgt tcgattcatt
    16261 cgtacttgtt acttgtcggt tcaaggaaga aatgattcct gaagtgacag aattcgcata
    16321 ttcagatcct aatggggaac caatcgcaat cgcaggttca ccaaccttca aagcacttga
    16381 gtccccaaaa gaggcaaccg tttcgacttt gtctgaattg atcttcaaca ccgctaaatc
    16441 tgaataggca tctgttccaa ctaattcagc ttttaccttc gtaccgtctt tcatcatgac
    16501 ttccaaacct tgctgtccat ctactacgtg attgtttgtg actacataag cagtattccc
    16561 gtcttttttg tagattacgc cgcttccttc actataagct tccaaattac tatcatctga
    16621 gctgctactt tcttcttgct gcccgaataa tccgccaaaa ccgcttgatt ggttttgact
    16681 ttgtaagttg ataacagaaa cgacagcgtc ttgaacttta tctaccgctt tggtgatatc
    16741 agaatctaca ttgactttga cattttccac aactgtttca ccggcagaat tttgattgcc
    16801 gcttgtagca gtagaactat tgttgccagt gcccatcgct gcataaaaaa tgccagctgt
    16861 cagtagtcct ccaacgatcc ctccaacaag ccccagaccc aacttacgcc agaggctatt
    16921 atttttattt tttttcatct ttggtgtcac attttttcta tccatgtgtt cctcctccaa
    16981 ttacattcat ttatctactg ttagtatagt cttatttgtt gactttagtc tactttgaga
    17041 ctttgaactt tttatgaaaa actttaggaa aaatcattgt tttcagatga aaatatttga
    17101 ttttcttttt ccacagatat tgtgtaaaac tacgcggata ggattggaaa aataagggat
    17161 aaaacatatc cacagcctgt gtataaagtg gataaattct tataaaacgt atgtttgcct
    17221 aatgaatgtg atgaaataag gtaaaacaac gctgttttct gtggataaac ctgtggatgt
    17281 tgtgcataac tgttggggaa atctttttaa caaaaatagt caaaataaac atatagacga
    17341 aaaaattgat tataataaac aaagtgagcg tgaagatcaa aggagagaaa acatgaatat
    17401 caaaattatt tcagtaggaa aattaaaaga aaaatacttg atccaaggta tcaatgagta
    17461 tgtcaaacgg ctgaatgcct atgcgaaaat cgaattgatc gaagttcctg acgaaaaagc
    17521 cccggagaat ttaagcgaag cgcaaatgag acaagtaaaa gaaaaagaag gagaacggat
    17581 cttagcgaaa atcaaagaac aagaatacgt ctatggctta gcaattgaag ggaaaaatcc
    17641 cacaagcgaa tcttttgcta aacaaatcga ccaattaggt atccaaggaa aaagccacct
    17701 tgtttttgtt atcggcggtt ctttaggact cagcgaagcc gtcatgaaaa gaagcaatgc
    17761 tcagatctct tttggcaaaa tgacgtatcc acatcaattg atgcgcctga tcctcgtcga
    17821 gcaaatctac cgtgccttcc ggatcaacgc gggggctcca taccacaaat aatgatattt
    17881 tttaagagaa aaatagcata aaaatctagt tatccgcata aaaactggac ttatcacact
    17941 ttatcaaggt caaaaccact caatttacta ctaatttact acttatgaat gagctttgat
    18001 acgacgattt atccttgaaa agtgaagata taaagatact tccaataaaa tttgaatatt
    18061 taataggtag acacttcaaa aaatgaggtg tctatttttt tacccgattt tgaaaggaag
    18121 tgaacttatg aaaacaaaaa atcaagaatc aaaaggtcgt tccccactct ttaagaccat
    18181 caaacattca ttcagccaat aaaaaagaaa ggataggtaa aaatatggaa cttaaatttg
    18241 tgattcccaa catggaaaaa acattcggca atttagaatt tgctggcgag gataaagtcg
    18301 ttcagcgaag aatcaacgga cggctaactg tcttatcaag aagctataat ctctattctg
    18361 atgttcaaag agcagatgat attgtggtgg tgcttcctgc tgaagctggc gaaaaacatt
    18421 tcggctttga ggaacgtgtg aagttagtca atccacgtat taccgcagag ggctacaaaa
    18481 tcggcactcg tggttttaca aattaccttt tacatgctga cgacatgata aaagaataaa
    18541 gaaagagagg aaaaatgatg agattagcaa atggcattgt attagataaa gacacgactt
    18601 ttggagaatt gaaattctct gctctacgtc gtgaagtgag aatccaaaat gaagacgggt
    18661 cggtttcaga tgaaatcaag gaacgtacct atgacttaaa atccaaagga caaggacgca
    18721 tgattcaagt aagtattcct gccagcgtgc ctttgaaaga gtttgattat aacgcacggg
    18781 tggaacttat caatcccatt gcggacaccg ttgctactgc cacctatcaa ggagcagatg
    18841 ttgactggta tatcaaggca gacgatattg tgctgacaaa ggattctagt tcattcaaag
    18901 ctcaaccaca agcaaagaaa gaaccgacac aagacaaata gtcgctaggt agaaaggaga
    18961 ctttttcgca tgaaacagcg tggtaaaagg attcgcccat ctggtaaaga tttagtcttt
    19021 cattttacga tagcgtcact cctgcctgtt ttcctgctgg ttgtcggact gtttcatgtg
    19081 aagacaatcc agcagatcaa ctggcaggat tttaacctat cacaagcaga taagattgac
    19141 attccctatt taattatcag tttcagtgtc gcaattctta tctgcttgct ggtagcgttt
    19201 gtattcaaac gggttcgcta tgatacggtt aaacaacttt accaccgtca aaaactggca
    19261 aagatgatac ttgaaaacaa gtggtatgaa tctgaacagg tcaaaacaga gggtttcttt
    19321 aaagatagtg ctggtcgtac aaaggaaaag ataacctact tccctaaaat gtattatcga
    19381 cttaaaaatg gcttgataca gatacgggtg gaaatcacgc tgggaaaata tcaagaccaa
    19441 ctcttacact tggaaaagaa attagagagt ggcttgtact gtgagctgac ggataaagag
    19501 ttaaaggatt cctatgtgga atatactttg ctctatgaca ccatagccag tcgtatttct
    19561 attgatgaag tagaagctaa agatggtaaa cttcgcttaa tgaaaaacgt atggtgggaa
    19621 tatgataagc tccctcatat gttgattgct ggtggtacag gtggcggtaa aacttacttt
    19681 atactgacac tgattgaagc cttgcttcat acagattcaa aactgtatat tcttgacccg
    19741 aaaaatgctg accttgcgga cttaggttct gtgatggcaa atgtctacta tagaaaagaa
    19801 gacttgcttt cttgcattga aacattctat gaagaaatga tgaaacgtag tgaggaaatg
    19861 aagcagatga agaactataa gactggcaaa aattatgctt acttaggtct cccggcacac
    19921 ttcttaatct ttgatgaata cgtcgctttc atggaaatgc tgggaacaaa agaaaacacc
    19981 gcagttatga ataagctgaa acagattgtc atgttaggtc gtcaagctgg cttctttcta
    20041 atactggctt gtcaacgtcc agacgcaaaa tatttaggcg acggaatccg tgatcagttt
    20101 aatttcagag tggctttagg tcgtatgtct gaaatgggct atggcatgat gtttggcagt
    20161 gacgtacaaa aggatttctt cttaaagcga atcaaaggtc gtggctatgt tgatgtagga
    20221 acaagtgtca tatcagagtt ttatactccc cttgtaccaa aaggatatga tttcttggag
    20281 gaaattaaaa agttatccaa cagcagacag tccacgcagg cgacgtgcga agcggaagtc
    20341 gcaggtgtgg actgatcttg ctggctggtg tggcaatagc cacgccagca cttaacccct
    20401 cggtatctaa caggggggta caaatcgaca ggaaacagtc aaaaaaacat tagaaaatcc
    20461 tttggttaca agggatttac aaaatttcag cgtatgtcaa atgggcttta aaagttgaca
    20521 tacgcctttt tgattggagg gatttttact gaatgaacaa acttggttac agcatttaaa
    20581 agaaaaacgc ttggcttatg gactatctca aaaccgttta gctgttgcga ctggtattac
    20641 aaggcagtat ctaagcgata ttgaaacagg aaaagtcaag ccatcagagg atttacagca
    20701 gtccctttgg gaagctctgg aacgcttcaa tcccgacgct ccccttgaaa tgctgtttga
    20761 ttatgtaaga attcgctttc cgacaacaga cgtacagcag gtggtcgaaa acatcttaca
    20821 actgaaactg tcctattttc ttcatgagga ctatggtttc tattcttatt cagagcatta
    20881 tgctttaggc gacatattcg tcctttgctc ccatgaactg gacaaaggag ttctggtgga
    20941 attgaaaggt cgtgggtgca gacaatttga aagctatctt ctggcacaac aaagaagctg
    21001 gtatgagttc tttatggacg ttttggtggc tggcggtgtg atgaaacgcc ttgaccttgc
    21061 cattaacgat aagacaggga ttttaaatat ccctgtactc actgaaaagt gccaacagga
    21121 agaatgtatc tccgtcttcc gcagttttaa aagctatcgc agtggcgaac tggtacgcaa
    21181 agaggaaaag gaatgtatgg gaaacaccct ctatatcggt tcattacaaa gtgaagttta
    21241 tttctgtatc tatgaaaagg actacgagca gtacaagaaa aatgatattc ccattgaaga
    21301 cgcagaagta aaaaaccgtt ttgagattcg attgaaaaat gagcgtgcct attatgcagt
    21361 ccgtgattta ctcgtctatg acaatccaga gcataccgcc tttaaaatta tcaatcggta
    21421 tatccgtttt gtagataaag acgattccaa acctcgttct gattggaaac tgaatgaaga
    21481 atgggcttgg tttattggga acaatcgtga acgattaaaa ctaaccacaa aaccagagcc
    21541 ttactccttc caaaggacgc tgaactggct atctcatcaa gttgccccga ccttaaaggt
    21601 tgcgattaaa cttgatgaaa tcaaccagac gcaggttgta aaagacattc tcgaccatgc
    21661 gaaactgaca gaccgacaca agcagatttt gaagcaacag tcagtaaaag aacaggacgt
    21721 gataacaaca aaaaaataac tcaaatacaa attcattgaa tatagagagg agaacatttt
    21781 tatgaatttt ggacaaaacc tttgtgcgac tcgttcctta gtgaaaagct aaggcactaa
    21841 taaaagaacg aaaaagtttg gttattagga gaactgataa tctgacaggt ggaatgatga
    21901 agtaacgctc tgaaacgcct gccctgagcc tccgacaagc attgtatgag gaaactcaga
    21961 atgtttgaag ctcggtaaag acggctgaaa gataccgtaa tagttgatga acatatatct
    22021 tctatcgaaa actatccaga ggtgggtagc gtatccaaca ggtcgggggt ctataaaata
    22081 tctatggtta gaatgttcta taaggaactg acgaaacagc gaatgtacgg gtctaaattt
    22141 gaactttgta gaaatgcaaa ggtacgagaa atcgtagctg gtgtggcaaa gtaagaatat
    22201 aggttatgaa atgccatata acgttacagg cggtatcaag ccagcaggct tatagctagt
    22261 gacctaagga tatatatgta tagataagat tatcggaacg aggaaaggta ttgggttctc
    22321 atcaagagaa taaactgacg aagaacaata agcagttgaa ctaatgctga aaagcagagg
    22381 gcgaacctaa gaaattcttg taatgagaat ggaggaatgc cctcaagtcg tgtatatatt
    22441 agaaaagtat tatgttgttt caataaggat tacgagtaag ataaaaaagc tcactcaatt
    22501 tcaagtaaag gagtgaagcc ttatgcctaa aaataaaaat gaaacattgt gtgtagaaga
    22561 tttaagacac gctgaatatt acgaaatgca aaacaccttt gatgatttat atgccaaaag
    22621 taagaatgga gatattttca ctcatcttat ggatattatc ctatcgagag aaaatatatt
    22681 actggcatat cgaaatatca aagcaaatgc aggcagtaaa acagcaggaa cagacggtac
    22741 aataattaaa gatattggca aattgccagc agaaacagtt gtcaaaaaag taagatacat
    22801 tgttgcaggc agtccacatg ggtatcgacc taagccagtg aggcgaaaag aaataccaaa
    22861 accaaatggt aaaaccagac ctctaggtat tccttgtatg tgggacaggc ttatacaaca
    22921 atgtataaaa caagtattag aaccaatctg cgaagctaaa tttagtgaaa acagctatgg
    22981 ttttagacct aataggtcag ttgaaaatgc aataaaagca acctataacc gcctacaaat
    23041 cagccagcta cattatgtga ttgaatttga tattaagggt ttctttgaca acgtgaacca
    23101 ttccaagctg ataaaacaga tatgggctat gggaatacgg gacaagcact tgatattcat
    23161 tttaaaaagg atattaaaag ctccaatcaa aatgactaac ggtacaataa catatccaga
    23221 gaaaggaact ccgcaagggg gaataatatc cccactacta gcaaacattg ttcttaatga
    23281 gttagaccat tgggtagaaa gccaatggca ggaaaatcct gttacaaaaa actatgtggt
    23341 tcatatcaat aaaagcggta gcccatgtaa aagcaacgct tacaaagaaa tgaagaaaac
    23401 aaagttaaag gaaatgtata tggttcgtta tgcagatgat tttagagttt tctgccgcta
    23461 caaggaaagt gctgaaaaag ccaaaatagc aatcactcaa tggattgaac aacgattaaa
    23521 attggaagta tcacaagaaa aaacaagaat tgtaaatgtt agaaaaagat actccgactt
    23581 tcttggtttc aagattaaaa tgataccgag aagaaagaag ttagttgtta aatcacatat
    23641 aagtgataaa caattcaaaa accaaaaaga caagctggta tcacaggcaa agaaaattgc
    23701 ttctccctca aaagggaaaa cggaattaga agaaacaaga ctttataatt caatggtatt
    23761 aggtatgcaa aattattatc agattgcaac gaatgtaaat ctggattgta tgaaactgaa
    23821 tagagctgta atggctatct tcactaacag attaggtaca aacaaaaggg gcagattgag
    23881 aaaaacaggc agacaactta cgacaaagga ggcaacaaga tatggtaaat ctaaaatgct
    23941 tcggtatgtt gctggcgagg gcgaacctat tttccctgtt ggatatatcc aacacagaaa
    24001 accaatggca aaggtgtatt tagcgaattg ctacactcta gagggaagaa aatttatcca
    24061 caccaatcta agtgttgata aattactgat gtaccagtta atgaaattat ctttggaaaa
    24121 tcgcacaata gaatacgcag acaatcgaat ttcgctattt tctgctcaac atggaaaatg
    24181 tgctataact ctggaagaat ttcaacaagc taatgaaata cattgtcatc atattgtacc
    24241 aactggagct ggtggaaatg acgattataa aaatcttatt ttggtaaaag aggcggtaca
    24301 ccgtctaata cacgctaaaa cagaagaaac aatacaaaaa tatatagctc tattgaagcg
    24361 aaataaagtc cagctcataa gactaaataa atatagagta ttagctggca atcaagaatt
    24421 aaacttgata taggtctact acataaatac ttttcaatat aaacacgatg gaacgcggag
    24481 tgcggtgaaa gtcgcacgct ccgtgtgaat ggggggaaaa gtttgagata tttataatat
    24541 caggaactta cctatccata tataactggt ttctatcaaa cgctcaatca ctggtgcttt
    24601 tagcaatcgt tgtgattggc ttgtatcttg gcttcaagcg tgagtttagc aaactgattg
    24661 gctttttaat tattgcgatt attgcggttg gcttagtctt caacgctgct ggagtaaaag
    24721 acattttact agagctattc aatcgcatta ttggtgctta aataaaaccg ttcttttgtg
    24781 gaatataagt ggttttctta tgttccgcaa aggaatggta caccaaacga agtgcggtag
    24841 ggatttttga atctctacaa agaaaggacg tgaatatatg gacgatatgc aagtctatat
    24901 tgcgaattta ggcaaataca atgagggcga attggtcggt gcgtggttta cctttcccat
    24961 tgactttgag gaagtcaaag agaaaatcgg cttgaatgat gaatatgagg aatacgccat
    25021 tcatgattac gagttaccct ttacggttga cgaatacact tctattagcg aactcaatcg
    25081 cctatgggaa atggtatcgg agttgccaga agaactacaa tcggagctat ctgctctgct
    25141 cactcatttt tcaagtattg aagaactaag cgaacatcaa gaagatatta tcattcattc
    25201 cgattgtgat gatatgtctg acgtggcacg ctactacatt gaagaaacgg gtgctttagg
    25261 cgaagtgcca gccagtcttc aaaactatat tgattatgaa tcctatggtc gggatttaga
    25321 cctttcggga acgtttatct caaccaatca cgggattttt gaaatcgtct attaaatctg
    25381 tcggtacatt cttactggca gattttcttg tttacggggt agtcaaaaga ctatccccat
    25441 ttttatgaaa ggattgatta catgaagaaa atacgaagct ataccagtat ctggtctgtt
    25501 gagaaagtgt tgtactccat caatgacttt agacttccgt ttcccataac ctttacgcaa
    25561 atgacatggt ttgtcgtgtc actctttgca gttatgatac ttggcaactt gccccctctt
    25621 tctatgatag agggagcatt tctcaaatac tttgggattc ctgtggcttt cacatggttt
    25681 atgtctacaa aaacctttga tggtaaaaag ccttatggat ttttgaagtc tgtcattgct
    25741 tatgcactgc gacgaaagct gacctatgca ggaaaaaaag taacgcttgg cagaaaccag
    25801 ccacaagaag ccattacagc agttaggagt gaattttatg gcatatccaa ttaaatatat
    25861 tgaaaacaat ctggtctgga ataaagatgg ggaatgttac gcttactatg agcttgttcc
    25921 ctacaattac tcatttctaa gtccggaaca gaaaatacaa gtacatgatt ctttcagaca
    25981 gcttatcgca caaaatcgtg atggcaagat tcatgcttta caaatcagta cagaatccag
    26041 catactttct gcacaagaac gttccaaaaa tgaagtcact ggaaagctca aaacggttgc
    26101 ctatgacaaa atcgaccaac agacagacgc tttaatatcc atgattggcg aaaatcaagt
    26161 ggactaccgt ttctttatcg gttttaagtt gcttctcaac gatcaggagt tttctatgaa
    26221 aagtcttacc gttgaagcaa aaaatgcttt tactgatttt gtctatgatg tgaaccataa
    26281 gctgatgggc gattttgtta gtatgagtaa tgatgaaatc ctgcgttttc agaagatgga
    26341 aaagctttta gaaaataaaa tctcccgtcg tttcaaaatc cgaagattag ataaggatga
    26401 cttcggctat ctgattgaac acctttacgg acagacaggc actgcctatg aagagtatga
    26461 gtatcctcta tcaaagaaaa agctggaaca tgaaacactg attaaaacct atgacctcat
    26521 taagcctact cgctgtctgg tggaagaaaa acagcgatat ttgaaaatcc agcaggaaga
    26581 cgaaaccgcc tatgtagctt actttaccat caacagtatt gtcggcgaac tggacttccc
    26641 gtcctctgaa atcttctact accagcaaca gcaatttacc tttccgattg atacgtcaat
    26701 gaatgtggaa attgtagcga atcgtaaagc cctatctact gtccgcaata aaaagaaaga
    26761 actgaaagac ttggataacc acgcttggca aagtgataat gaaaccagct ctaatgtggc
    26821 ggaagctctg gaaagtgtga acgagctgga aaccaattta gaccaaagca aggaatctat
    26881 gtataagctg tcctatgttg taagggtatc agcaaatgac cttgacgaac ttaaacgtcg
    26941 ttgtaatgaa gtgaaagatt tctatgatga tttgagcgtg aaactggtac gaccttttgg
    27001 ggatatgctg ggcttacatg aagaattttt acctgccagc aaaagatata tgaatgacta
    27061 tattcaatac gtgacctctg atttcctcgc tggtttaggt tttggtgcta ctcaaatgct
    27121 gggtgaaaat gaggggattt atgttggcta cagcttagat actggacgca atgtctatct
    27181 gaaacctgct cttgccagtc aaggggttaa gggttcagta accaatgcgt tagcgtctgc
    27241 ctttgtcggt tcgctgggtg gtggtaaatc ctttgcgaat aaccttatcg tctattatgc
    27301 agtgctttat ggggcacaag cagtgattgt agacccaaaa gcagaacgtg gcagatggaa
    27361 agaaaccttg ccagagattt cccatgaaat caatatcgtc aatctgactt ctgatgagaa
    27421 aaacaaaggc ttacttgacc cttatgtaat tatgaaaaat accaaagatt ctgaatcact
    27481 ggctattgat attttgacat tccttacggg gatttcctct cgtgatgggg aacgcttccc
    27541 aatccttaga aaagccattc gtgcagtaac caatagtgaa gtgcgagggt tgatgaaagt
    27601 gattgaagaa ttacgggttg agaatacgcc actaagtacc agcatagccg accatatcga
    27661 aagttttaca gactatgact ttgcccatct gctttttagt gatggttatg tagagcagtc
    27721 tatcagcctt gaaaaacaac tgaacattat acaggttgcc gacttggtac ttcctgacaa
    27781 ggaaacttcc tttgaggaat ataccacaat ggagttacta tcggtagcaa tgctgattgt
    27841 cattagtacc tttgcgttgg actttatcca tacagaccga agtattttca agattgtaga
    27901 cttagacgaa gcatggagct tcttacaggt ggcacaaggg aaaacactat ctatgaagct
    27961 ggttcgtgct ggtcgtgcta tgaacgctgg ggtatatttc gtgacccaaa atacagatga
    28021 cctcttagat gaaaaactga aaaataacct cggcttaaaa tttgcctttc gttccactga
    28081 ccttaacgag attaaaaaaa cccttgcctt ttttggtgta gacccagagg acgaaaacaa
    28141 tcagaagcga ttgcgtgatt tggaaaacgg gcaatgcctt atcagtgatt tatatggtcg
    28201 tgtcggtgtg atacagttcc accctgtatt tgaagaactg ctccatgcct ttgataccag
    28261 accacctgtg cgaaaagagg tgtaaatgtg aaaccatcaa tattaaacag aataaaatca
    28321 aactggacgc tgaaacgtct aggtaaagtg gcaatgacag tggctttcac acttgtgatt
    28381 gccatttttc ttttagccat gctgggaacg gtggttcaag ctgcgggctt ggtagatgat
    28441 acggtcaatg tggcaaatga atacagccga tacccacttg aaaactatca actggatttt
    28501 tatgtggata atagctgggg ctggctaccg tggaactggt cggacggaat tggaaaacaa
    28561 gtcatgtatg gactatatgc cattaccaat tttatttgga caatcagtct ttatgtttcc
    28621 aatgcgacgg gttacttagt acaggaagcc tattccttag acttcatttc cgctacagca
    28681 gattccattg gcaagaatat gcagaccctt gcaggggtta cgcctagtgg actatcaaca
    28741 gagggtttct atgttggatt ccttttactc ttgattttgg ttcttggggt ttatgttgcc
    28801 tatacaggac tgataaagag agaaaccaca aaggcaattc atgccctcat gaattttgtg
    28861 ctggttttta tcctatcggc ttcctttatt gcctacgctc ccgactacat taaaaaaatc
    28921 aatgactttt catcagacat cagcaacgct agtttgtcac tgggtacaaa aattgtcatg
    28981 ccccattcag atagtcaagg caaggacagt gtggacttga tacgtgacag cctattttcc
    29041 atacaggttc agcaaccgtg gctactgctt caatataaca gttcagacat tgaaagtatt
    29101 ggtattgacc gtgtggaaag cctgctctca accagtccag attccaacaa tggggaagac
    29161 agagaaaaaa ttgttgcgga agaaattgaa gatagaagca ataccaatat gacgattaca
    29221 aagacgatta accgtttagg tacagtcttc ttcctatttg tcttcaatat tgggatttcc
    29281 atatttgtat tcctattaac aggaatcatg attttctcgc aggtactttt tatcatctat
    29341 gctatgtttc tgcctgtgag ctttatttta agcatgattc catcatttga tggtatgtca
    29401 aaacgagcca taacaaagct ctttaatacc attttgacac gagctggaat cacattgatt
    29461 attacgacag catttagtat ttcaaccatg ctctatacct tatcggctgg ttatccgttc
    29521 tttttgattg cttttctaca gattgtgacc tttgcaggaa tctactttaa gctgggcgat
    29581 ttaatgagta tgttttctct acagagtaac gattctcaaa gtgtgggaag tcgtatgatg
    29641 agaaaacctc gtatgcttat gcacgctcac atgcaccgtc tacagcggaa acttggacgc
    29701 tccgtgactg ctttaggggc tgggtctgcc attgccagtg ctacgggtaa aaaaggacag
    29761 tcgggttcgg gaagttctgc gagtacacaa gcagatcact ctcgaccaaa cgggcaggaa
    29821 aaatcaacac taggaaagcg tatcggtcaa accatcggta cagtagctga taccaaagac
    29881 agaatggtag atactgctgg taatttgaaa gaacaggtta aggatttgcc gaccaatgca
    29941 agatatgcag tatatcaagg aaaatctaaa gtgacggata atgtccatga tttaaccaga
    30001 agtatttctc aaaccaaagc ggataaagcc agtggacgca agaaacagca ggaacaaaga
    30061 cggaaaacca ttgcggagcg tcgctctgaa atggaacagg tcaaacagaa aaaacagcca
    30121 gcttcttccg ttcatgaaag accagccact aagcaagaac aatctcatga tggacagact
    30181 tcaaggcaaa caactgtaca ggcttcctat agggaatctc aacaagccaa acaagagcgt
    30241 ccaacagtta agtccgattc ttcaagttta aaaacggaac gtcaaagtaa tacagttcaa
    30301 gaaagaacgg ttcaaaagcc agtaacttca actgcaccaa cagatagagc ttcacaacgt
    30361 tcaatcacaa aagaacgtca gtctactgtt caaagagtac cactacaaaa tacaaaaagt
    30421 agaccaccaa tcaaaaccgc caccattaag aaaggcagta agaaaccatg aagctgaaaa
    30481 ctttagtgat tggtggttcg ggattattct tgatggtctt ctcactgctt ctgtttgtcg
    30541 ccattttatt ttcagatgaa caagatggcg gtttttccaa cattcactat ggcggtgtgg
    30601 atgtttccgc agaagtgctg gctcataagc ctatggtaga aaaagttgcc aaagaatatg
    30661 gaattgaaga atacgtcaat atacttcttg cgattataca ggtggaatcg ggtggtacag
    30721 cagaagatgt tatgcagtcc tcggaatccc tcggtcttcc acctaattca ttgagtacag
    30781 aagaatccat taagcaaggt gtgaagtatt tcagtgagtt attagccagt agcgaaaggc
    30841 ttggtgtaga tttagaatcg gttatccagt cctacaatta tggtggtggt ttcttaggat
    30901 atgtggctaa tcgtgggaat aaatatacct ttgaactggc tcaaagtttc tcaaaagagt
    30961 attcgggtga cgaaaaagtg tcttacccca atcccatagc catacctatc aatgggggct
    31021 ggcgatacaa ttacgggaat atgttttatg tgcaactggt aacgcagtat cttgtcacaa
    31081 cagagtttga tgatgataca gtacaagcca tcatggacga agctctgaaa tttgaaggct
    31141 ggaagtatgt ctatggcgga gcttccccga ctacctcttt tgattgtagc ggactgacac
    31201 aatggacgta tggaaaagct ggaatcaact taccacgaac agcacaacag caatatgatg
    31261 taacccggca tatcccatta ttggaagcaa aagccggcga tttggttttc tttcattcta
    31321 cctataacgc tggctcttat attactcata tcgggattta tttaggcgat aaccgtatgt
    31381 ttcatgcagg cgaccctatc ggttatgccg acttaacaag ctcttactgg caacagcatt
    31441 tagtgggagc aggacgaatc aaacaataag aaaggacgat tcaatgatga aattcggaaa
    31501 aaatcagaat acagaaaaac agacaccaaa ggaaaagaaa cctcgtgtct ataaggtcaa
    31561 tcctcgtaaa aaggttgtga ttgtcctatg ggtactttta ggacttagtt tcagctttgc
    31621 gatattcaag cactttacag ccattgatac ccataccatt catgaaacca ccatcataga
    31681 aaaggaatat gtcgatactc atcatgtgga aaattttgta gagaactttg cgaaagtcta
    31741 ctattcatgg gaactaaacg acaagtccat tgataatcgc atagaaaacc tgaaagcata
    31801 tctgacagag gaacttcaag ctctgaatgt cgatacagtt cgtaaagata ttcctgtatc
    31861 gtcttctgta aaaggatttc agatatggac ggtagaagca gatggcgaca atcaatttga
    31921 tgtaacctac agtgtagacc aactcattac agagggggaa aatacaaaga ccgtccactc
    31981 tgcttatata gtgagtgtct atgtagacag tactggaaat atggtcctca ttaagaatcc
    32041 gaccattact agcataccaa agaaatcaga ctataaacca aaagctattg aaagtgaggg
    32101 tacggttgat tccattacaa ccaatgaaat caatgagttt ttaacgacgt tcttcaagct
    32161 ctatcctaca gcgacagcca gtgaactttc ctactatgtg aatgacggga tattaaaacc
    32221 aatcggaaaa gagtacatct ttcaagaact ggtaaatcct atttacaatc gtaaggataa
    32281 tcaagtcacg gtatcgctga cagtggagta tatcgaccag cagaccaaag caacgcaggt
    32341 atctcaattt gatttggtac ttgaaaagaa cgggagtaat tggaagatta tagaataaca
    32401 aatattggta cattattaca gctattttgt aatcacgtac tctctttgat aaaaaattgg
    32461 agattccttt acaaatatgc tcttacgtgc tattatttaa gtatctattt aaaaggagtt
    32521 aataaatatg cggcaaggta ttcttaaata aactgtcaat ttgatagtgg gaacaaataa
    32581 ttggatgtcc ttttttagga gggcttagtt ttttgcaccc agtttaagaa tacctttatc
    32641 atgtgattct aaagtatccg gagaatatct gtatgctttg tatgcctatg gttatgcata
    32701 aaaatcccag tgataagagt atttatcact gggattttta tgcccttttg ggcttttgaa
    32761 tggaggaaaa tcacatgaaa attattaata ttggagtttt agctcatgtt gatgcgggaa
    32821 aaactacctt aacagaaagc ttattatata acagtggagc gattacagaa ttaggaagcg
    32881 tggacaaagg tacaacgagg acggataata cgcttttaga acgtcagaga ggaattacaa
    32941 ttcagacagg aataacctct tttcagtggg aaaatacgaa ggtgaacatc atagacacgc
    33001 caggacatat ggatttctta gcagaagtat atcgttcatt atcagtttta gatggggcaa
    33061 ttctactgat ttctgcaaaa gatggcgtac aagcacaaac tcgtatatta tttcatgcac
    33121 ttaggaaaat ggggattccc acaatctttt ttatcaataa gattgaccaa aatggaattg
    33181 atttatcaac ggtttatcag gatattaaag agaaactttc tgccgaaatt gtaatcaaac
    33241 agaaggtaga actgtatcct aatatgtgtg tgacgaactt taccgaatct gaacaatggg
    33301 atacggtaat agagggaaac gatgaccttt tagagaaata tatgtccggt aaatcattag
    33361 aagcattgga actcgaacaa gaggaaagca taagatttca gaattgctcc ttgtaccctg
    33421 tttatcatgg aagcgcaaaa agcaacatag ggattgagca gcttatagaa gtgataacga
    33481 ataaatttta ttcatcaaca tacagaaaga agtctgaact ttgcggaaat gtcttcaaaa
    33541 ttgaatattc ggaagaaaga caacgtcttg catatgtacg cctttatggc ggaatcctgc
    33601 atttgcggga ttcggttaga atatcggaaa aggaaaaaat aaaaattaca gaaatgtata
    33661 cttcaataaa tggtgaatta tgtaaaattg ataaggctta ttccggggaa attgttattt
    33721 tgcaaaatga gtttttgaag ctaaatagtg ttcttggaga tacaaagcta ttgccacaga
    33781 gagagagaat tgaaaatccg ctccctctgc tgcaaacaac tgttgaaccg agcaaacctc
    33841 aacaaaggga aatgttactt gatgcacttt tagaaatctc cgacagtgac ccgcttctac
    33901 aatattatgt ggattctacg acacatgaaa ttatactttc tttcttaggg aaagtacaaa
    33961 tggaagtgat tagtgcactg ttgcaagaaa agtatcatgt ggagatagaa ctaaaagagc
    34021 ctacagtcat ttatatggag agaccgttaa aaaatgcaga atataccatt cacatcgaag
    34081 tgccgccaaa tcctttctgg gcttccattg gtttatctgt atcaccgctt ccgttgggaa
    34141 gtggaatgca gtatgagagc tcggtttctc ttggatactt aaatcaatca tttcaaaatg
    34201 cagttatgga agggatacgc tatggttgcg aacaaggatt atatggttgg aatgtgacgg
    34261 attgtaaaat ctgttttaag tatggcttat actatagccc tgttagtacc ccagcagatt
    34321 ttcggatgct tgctcctatt gtattggaac aagtcttaaa aaaagctgga acagaattgt
    34381 tagagccata tcttagtttt aaaatttatg cgccacagga atatctttca cgagcataca
    34441 acgatgctcc taaatattgt gcgaacatcg tagacactca attgaaaaat aatgaggtca
    34501 ttcttagtgg agaaatccct gctcggtgta ttcaagaata tcgtagtgat ttaactttct
    34561 ttacaaatgg acgtagtgtt tgtttaacag agttaaaagg gtaccatgtt actaccggtg
    34621 aacctgtttg ccagccccgt cgtccaaata gtcggataga taaagtacga tatatgttca
    34681 ataaaataac ttagtgtatt ttatgttgtt atataaatat ggtttcttgt taaataagat
    34741 gaaatatttt ttaataaaga tttgaattaa agtgtaaagg aggaattaat tatgaagtgt
    34801 aatgaatgta acagggttaa atacaaccaa attttaattt ggctttgcgt tttatctttt
    34861 tttagcgtat taaatgaaat ggttttgaac gtctcattac ctgatattgc aaatgatttt
    34921 aataaaccac ctgcgagtac aaactgggtg aacacagcct ttatgttaac cttttccatt
    34981 ggaacagctg tatatggaaa gctatctgat caattaggca tcaaaaggtt actcctattt
    35041 ggaattataa taaattgttt cgggtcggta attgggtttg ttggccattc tttcttttcc
    35101 ttacttatta tggctcgttt tattcaaggg gctggtgcag ctgcatttcc agcactcgta
    35161 atggttgtag ttgcgcgcta tattccaaag gaaaataggg gtaaagcatt tggtcttatt
    35221 ggatcgatag tagccatggg agaaggagtc ggtccagcga ttggtggaat gatagcccat
    35281 tatattcatt ggtcctatct tctactcatt cctatgataa caattatcac tgttccgttt
    35341 cttatgaaat tattaaagaa agaagtaagg ataaaaggtc attttgatat caaaggaatt
    35401 atactaatgt ctgtaggcat tgtatttttt atgttgttta caacatcata tagcatttct
    35461 tttcttatcg ttagcgtgct gtcattcctg atatttgtaa aacatatcaa gaaagtaaca
    35521 gatccttttg ttgatcccgg attagggaaa aatatacctt ttatgattgg agttctttgt
    35581 gggggaatta tatttggaac agtagcaggg tttgtctcta tggttcctta tatgatgaaa
    35641 gatgttcacc agctaagtac tgccgaaatc ggaagtgtaa ttattttccc tggaacaatg
    35701 agtgtcatta ttttcggcta cattggtggg atacttgttg atagaagagg tcctttatac
    35761 gtgttaaaca tcggagttac atttctttct gttagct
//
LOCUS       DAXWOO010000009        35450 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.009, whole genome shotgun sequence.
ACCESSION   DAXWOO010000009 DAXWOO010000000
VERSION     DAXWOO010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.009"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            25..696
                     /locus_tag="ACGE70_000362"
     CDS             25..696
                     /locus_tag="ACGE70_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFF8692030.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            755..1195
                     /locus_tag="ACGE70_000363"
     CDS             755..1195
                     /locus_tag="ACGE70_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692031.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1207..1875
                     /locus_tag="ACGE70_000364"
     CDS             1207..1875
                     /locus_tag="ACGE70_000364"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFF8692032.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1939..2562
                     /locus_tag="ACGE70_000365"
     CDS             1939..2562
                     /locus_tag="ACGE70_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFF8692033.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2618..3004
                     /locus_tag="ACGE70_000366"
     CDS             2618..3004
                     /locus_tag="ACGE70_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFF8692034.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3217..4608
                     /locus_tag="ACGE70_000367"
     CDS             3217..4608
                     /locus_tag="ACGE70_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFF8692035.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            5219..5587
                     /locus_tag="ACGE70_000368"
     CDS             5219..5587
                     /locus_tag="ACGE70_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFF8692036.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            5767..6708
                     /locus_tag="ACGE70_000369"
     CDS             5767..6708
                     /locus_tag="ACGE70_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFF8692037.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFIALIK"
     gene            6777..7508
                     /locus_tag="ACGE70_000370"
     CDS             6777..7508
                     /locus_tag="ACGE70_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFF8692038.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            7652..9832
                     /locus_tag="ACGE70_000371"
     CDS             7652..9832
                     /locus_tag="ACGE70_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFF8692039.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            9852..10334
                     /locus_tag="ACGE70_000372"
     CDS             9852..10334
                     /locus_tag="ACGE70_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFF8692040.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            10350..11111
                     /locus_tag="ACGE70_000373"
     CDS             10350..11111
                     /locus_tag="ACGE70_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFF8692041.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            11242..12576
                     /gene="murC"
                     /locus_tag="ACGE70_000374"
     CDS             11242..12576
                     /gene="murC"
                     /locus_tag="ACGE70_000374"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFF8692042.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            12883..13368
                     /locus_tag="ACGE70_000375"
     CDS             12883..13368
                     /locus_tag="ACGE70_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFF8692043.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            13361..14044
                     /locus_tag="ACGE70_000376"
     CDS             13361..14044
                     /locus_tag="ACGE70_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFF8692044.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            14170..16815
                     /gene="polA"
                     /locus_tag="ACGE70_000377"
     CDS             14170..16815
                     /gene="polA"
                     /locus_tag="ACGE70_000377"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFF8692045.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            16860..17696
                     /gene="mutM"
                     /locus_tag="ACGE70_000378"
     CDS             16860..17696
                     /gene="mutM"
                     /locus_tag="ACGE70_000378"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFF8692046.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            17660..18289
                     /gene="coaE"
                     /locus_tag="ACGE70_000379"
     CDS             17660..18289
                     /gene="coaE"
                     /locus_tag="ACGE70_000379"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFF8692047.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            18341..18673
                     /locus_tag="ACGE70_000380"
     CDS             18341..18673
                     /locus_tag="ACGE70_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFF8692048.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            18985..19476
                     /gene="nrdR"
                     /locus_tag="ACGE70_000381"
     CDS             18985..19476
                     /gene="nrdR"
                     /locus_tag="ACGE70_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFF8692049.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            19499..20872
                     /locus_tag="ACGE70_000382"
     CDS             19499..20872
                     /locus_tag="ACGE70_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFF8692050.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            20872..21798
                     /gene="dnaI"
                     /locus_tag="ACGE70_000383"
     CDS             20872..21798
                     /gene="dnaI"
                     /locus_tag="ACGE70_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFF8692051.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            21927..22367
                     /locus_tag="ACGE70_000384"
     CDS             21927..22367
                     /locus_tag="ACGE70_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFF8692052.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            22511..23212
                     /locus_tag="ACGE70_000385"
     CDS             22511..23212
                     /locus_tag="ACGE70_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFF8692053.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            23209..24330
                     /locus_tag="ACGE70_000386"
     CDS             23209..24330
                     /locus_tag="ACGE70_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFF8692054.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            24345..25577
                     /gene="pepT"
                     /locus_tag="ACGE70_000387"
     CDS             24345..25577
                     /gene="pepT"
                     /locus_tag="ACGE70_000387"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFF8692055.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            25709..26617
                     /locus_tag="ACGE70_000388"
     CDS             25709..26617
                     /locus_tag="ACGE70_000388"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFF8692056.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            26652..26924
                     /locus_tag="ACGE70_000389"
     CDS             26652..26924
                     /locus_tag="ACGE70_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFF8692057.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            26935..27981
                     /locus_tag="ACGE70_000390"
     CDS             26935..27981
                     /locus_tag="ACGE70_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFF8692058.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            28232..30841
                     /gene="clpB"
                     /locus_tag="ACGE70_000391"
     CDS             28232..30841
                     /gene="clpB"
                     /locus_tag="ACGE70_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFF8692059.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(30992..31678)
                     /locus_tag="ACGE70_000392"
     CDS             complement(30992..31678)
                     /locus_tag="ACGE70_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692060.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(31656..32150)
                     /locus_tag="ACGE70_000393"
     CDS             complement(31656..32150)
                     /locus_tag="ACGE70_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFF8692061.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(32170..33390)
                     /locus_tag="ACGE70_000394"
     CDS             complement(32170..33390)
                     /locus_tag="ACGE70_000394"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8692062.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            33537..35372
                     /gene="lepA"
                     /locus_tag="ACGE70_000395"
     CDS             33537..35372
                     /gene="lepA"
                     /locus_tag="ACGE70_000395"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFF8692063.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
BASE COUNT        11869 a         6249 c         7624 g         9708 t
ORIGIN      
        1 aatgatgaaa aaagggggag aaaaatgaat tttttagttg ttttaaaaga tactctgatt
       61 gagcgatcta catttctata tgttcaattg caggaaaata aaacattgct gcatttttca
      121 ccagagttcc atctagaggg gctaacatta ggagaaatct atcaagcaga agggctgtcg
      181 gctgttttgc acttttttaa ggtagaattg gaattaccag taaaagatta tatcgagctt
      241 tctcttgaat cttgggatcg agcagtggtt gaactgtttc cagaaggttt aatgatcgat
      301 acaaacgatg ggaagatcca tctgactgca gcagaactgc aaaaacaaat gcgttatcaa
      361 atagatgacg aaaattcttt ttctattttt agacgtcagc aaaagattct aaaaagcttt
      421 ttaaaagtga tcagtaaaaa acggaatctg atcaaaacga cacagctgtt gaaaaaatat
      481 ccaggtctgc ttcacacgtc tattcaattt cctcaattga ttacgatgat ccatcgtttt
      541 atacgtatgc aacagctacc atctaagaag ctgtttcttc cattgacgga cacatttcga
      601 gtagtcaaca gagaagataa aaagaaagtg attgtgattg actttaccag aaatcggaat
      661 gtccttcacc aagttgcaat ggaaaaggcc aattaaggct ttttcttttt ggattttttt
      721 gtcataataa atattagatg atggaggaga taatatgatg caaacatggt tagaaaggct
      781 aatacgattt cagcagaact atcacaaact tatgcaggga agatatgcga aatttgatca
      841 gttgaatcgc ggtctattga tcgctgcgct tgtctttagt ttgctaagtc gttggttgcc
      901 ctatagaatc ggacaattgt tgttcatcgt ctttttcgga tggatgatat ttcgtttcct
      961 atcaaaaaaa atctacccca gactaaatga gaatcaacgt tatttgaaat atcttgatag
     1021 agtcaaagaa aaatatcatc agataaaaaa caaaaagcaa gtcaaaatgg atcgaaaaag
     1081 ttatactttt tttgagtgcc cgaattgtca tcaaaagcag cgtgcaccta aaggaaaagg
     1141 acgaatacgt gtgacatgca aaacatgtgg tataaaattc gaaacgaatg tttaagggag
     1201 aaaagaatga aattagtatt attacgtcat ggcgaaagcg aagcaaattt tgaaaattac
     1261 tggacaggat ggctagatgt cgccttaaca gaaaaaggaa aagaacaagc agcagttgcc
     1321 ggtcaaaaaa tgcttgaagc cggacttcaa ttcgatgccg tttatacctc tgttttgaaa
     1381 cgtgcgattc ttaccggaca agtcgcacta gaagaaatgg gtcagttatg gcttcctata
     1441 ataaaaacat ggcgtctaaa tgaacggcat tatggggatc ttgtgggtaa aaacaaagat
     1501 aagatgaaag aaatatacgg aaaagatcaa gtaaaaacat ggcgcagagg atatacggaa
     1561 gttccgccgt taaccgcaga aaatagattt gaccgcagat atgatcactt ggatccgcat
     1621 ttgatcccat acggagaaag tctggaaatg acagtgaatc ggatcattcc cttatggcaa
     1681 gatcatctag caccaaaact aaaagatcgg caagatttac tagttgtggg tcatggtaat
     1741 agcatccgag cactgacaaa atatttagaa gatgttcctg aagatcaaat ggatacgatc
     1801 gatatcccaa atgcacaacc aatccagtat acattagcag atgatttgac aattctggac
     1861 aaaaaaatac tttaaagaac acctttatct aaaagtgaga gatagaaaaa agaagcaaag
     1921 gaatggaaca aaaggaacat gaaaaattat atatgggact ttgatggtac attgtttgat
     1981 acgtatcccg caatggtaga tggtgcatgg caagcactaa aagattttgg tatcagtatg
     2041 gacaaaaaag aaatctactt caaaatgaaa aagtattcta ctagttattt gatcaatgaa
     2101 tcaaacttga atgccagaga gtttaatgaa ttatttcacc gatatgaaaa agaaagtacg
     2161 gaagtttccc gtccatttcc agaaacgaaa caagtactgg aaatgctaaa agacaacggc
     2221 ggtcgtcatt tcatcctgac ccaccgtttg acagaatcaa catggggcct gttaaaagaa
     2281 catcgattgg cacatctgat tgaggaagta gtagggatcg atcaggactt tcctcgtaaa
     2341 cctgatccag catctttaaa ttatttgatc gatacttttc atttagaaag aacagacacg
     2401 atgatgatcg gcgatcgtcg tttagatatc gaagcaggga aaaatgcagg agtcgctact
     2461 tgtctttacg acatcgatca ttttctgggt gagatcccgg cagattatgt ggtaggaaat
     2521 ctgaatgaaa tccttacctt gcagagcatc attaaaaagt agtatcccct ttcaaaggag
     2581 atatgttata attttcataa aggagtgata aaattatatg gaaatcagaa gacaaaaaga
     2641 agttgtagaa gcatttcatt atgatatgag attgccgaac caagaagtag aaacagattt
     2701 gaaagtaggc ttctcgcctt taaaagaaac gatcgaaaac tatccgaaag aacatagcat
     2761 cgtaggagca cgcttagagt tccgtttagt gtttgataca tatgttttat ctggaagtgt
     2821 gagtcagatc aatcatatcc tcaatcggaa aattgaaggt caaaatgatg tcacacaaga
     2881 agaagtggac gagctagtag caccgttatt cgatatcgta cgccgaatgt cttatgaggt
     2941 gacagagatc gccttagatc agcctggggt aaaattgaat tttcaatcag aaaaacaaga
     3001 atagacagta aaaacatacc tgcgatagat taaagctatc acaggtatgt ttttttatta
     3061 ctaattgtaa gcaaaaagaa tgtcatttcg ataaaaatcg tctaaaatat aaaagtcact
     3121 gaaaaaaatt ttttcaggag gatggttgaa gaatcattct cgggaacctt tacttccttc
     3181 gatggacttg tcattaataa tatgaggagg agaaaaatga caacactcat cacagtttta
     3241 gtggttctag tttttgcggc attattatat gtgttctatc gtatgcaaaa gcgccataca
     3301 aaattttcaa cacgcgtctt tacagcatta ggagttggta tcgtctttgg cggtctgctt
     3361 caattgatct ttggtgtaga cgacaaagtc gttactcaat caatggaatg gatcgggatt
     3421 gtaggtcaag gatatgtatc attcttgcaa atgctagtta tgcctttagt gtttatttcg
     3481 atcattggtg cattcacaaa aatgaaagtt tctgaaaagc tagggaaaat tagtgcgact
     3541 gtattgactt ctttacttgg aacaacagct attgcagcat tgatcggaat tggttcaaca
     3601 atgctgttcg gactccaagg agctaaattc acacaaggag ctgcagaaac agcacgaatc
     3661 tctgaactgg caacacgtca ggaaacagtt gaaaacttat cgattccaca gcagatttta
     3721 tcctttatcc ctaaaaacgt ttttgctgat ttagcaggta gtcgtgcaac aagtacgatt
     3781 ggagtcgtta ttttcgcggc atttgtcgga atcgcttatc taggcgtgaa aagaaaagcg
     3841 ccaaaagaag cagcattctt ttcagattta gtcgacagtc tgtataaaat cattatgcgg
     3901 atcgtgacgc ttgttttgcg tttgacacct tatggagtat ttgctttgat gacaaacgtt
     3961 ttggcaacaa gtgattttgc agctatcgtc aatttaggta aatttgtcgt tgcttcttac
     4021 gtagcattgc ttgctgtttt gattgtccac tcactgattt tgattggtgc aaaagtcaat
     4081 ccaatcaact atttcaaaaa agcctttccg gtattgagct ttgcgtttac ttcacgttca
     4141 agtgctggag ctttgccatt gaatatcgag actcagacaa aagctttagg agtagatgat
     4201 gcaacagcta atttctcagc aagttttggt ttgtctattg gacaaaacgg ctgtgctggc
     4261 gtttatccag ctatgttggc aacgatcgtc gcaccagcag ctggagtgaa tgtctttagt
     4321 ttggaattta ttttgatgct tgtggcaatt gtaacaatca gttcttttgg tgtagctgga
     4381 gtcggtgggg gcgcaacatt tgcttcactg atcgttttag gggcaatgaa cttgccagta
     4441 gcaatcgttg ggttagttat ctctgttgaa ccattaatcg atatggcacg tacagcagta
     4501 aacgtcagcg gtagcatggt agctggtatc gtgacaagtg cacgcatcaa agatttagat
     4561 cgcgatgtca tcaatgatga ttctaaagtg atcgatgtga atgcatagtc aaaaagaggt
     4621 tgtgatagaa gtggtcagct ttaagaaata agaagaagtt tcctaaaaat agtttctcaa
     4681 atttttgtga aatttcggct tatttccgag aagctacttc tgaaacaccg tgaataagag
     4741 gttgtgaaaa agctgccctg catcaagtaa taagaacagc caaacaaaaa tagttcctca
     4801 tattttacgt ttggttgagc ttattaccgc agatgcaagc ttttgaacac cgtttaagaa
     4861 agaggtcgtg agcctgccgt ttcactctga gtcacaaaga gcaatttcaa aaaatagctt
     4921 ctcatgtttg ttgaatattg tgtcttgtga ccgaggagtt gtcaggagaa caccgtttac
     4981 cagaggttgt gacttgtttt tagtcacgac ctcttttttt atttattcca tataaataca
     5041 cattatttat tccgaaaagt gaaagcgcaa ataacaaaaa caaccaactt atttattatt
     5101 aaagaaaagc gtatttaagt aggacgattc tgttgagagc tttcgtagat tttcccattt
     5161 tcctatggta aactgacaaa gttgagaatg ttagaacaga tcgtttagga ggaaacagat
     5221 gtttacgaat gaaataaaaa tcatgcttta tgtggacgat gtagaaaaaa atgcaacgtt
     5281 ttggagagca gttggctttg cggaaaaaga tcgtcaagaa atggatggga cgcttataat
     5341 tgaagtagga gttgaggaca gtcaaacctc atttctgtta tatgatcgag catttgttga
     5401 gtcccattca cctggcacag ctactagtgc cccatctttg atgttttaca gtaaagatat
     5461 ctttggacta tataaaaaaa tgcagacgca atcagtcaca ttaggtgata tggtacagct
     5521 tggagaagaa tatgtcttta actttgctga tcccgatggc aactactttg cggttacagg
     5581 taaataatag aaataaagac tttgtttttc tgtgcgaaac aggaaagcga agtcttttta
     5641 attcaaataa taatattatg taaacaaaaa actttccatg cgtaattata cacaagcaaa
     5701 taaatcgtca aatgcgaacg ccttctttat actgtttagg aacgaaaaga aggaaggaag
     5761 aaatacatgg attatcaagt actgctttat tacaattata caaaaatcga agaaccagag
     5821 gaatttgcca aaaatcattt agccttttgc aaatctttgg atcttaaagg tcgtattcta
     5881 gtagctgaag aaggaataaa tggtactgtg tctggaaccg ttgaaaacac gactgcctat
     5941 atgaaagcaa tgaaagctga tcctcgattt gctgatacga tgttcaagat cgatccaagc
     6001 gaaggacatg cctttaagaa gatgtttgta agataccgtc cagagcttgt aagtttaaag
     6061 ctagaagagg acattgatcc aaaccaagtg acaggtgcct atctttcacc gcaagaattt
     6121 aaagaagcta ttttagatga acagacagtg gtgattgatg cacggaatga ttatgaatat
     6181 gacttgggac attttcgagg ggctgtacgc ccagatatac gcagttttcg agagcttcca
     6241 gactggatcc gcgagcacaa agaagaattt atggaaaaac gggtagtgac ctactgtact
     6301 ggtggcattc gttgcgagaa gttttctggt tggcttgtcc gtgaaggatt caaagatgtc
     6361 ggacagctgc atggcggtat tgctacttat ggtaaggatc ctgaagtcca aggagaatta
     6421 tgggacggca agatgtatgt gtttgatgaa cggatcgctg tcgatatcaa ccaagtagat
     6481 aaacaaatcg tcggtcgcga ctggtttgac ggaacaccat gtgaacgata tatcaattgt
     6541 agcaatcctg agtgcaatcg tcagattctt gcttccattg aaaatgaaga aaaacattta
     6601 ggagcatgta gcatagagtg tgcgaaacat ccgcgcaatc gctatgttca agaccatgga
     6661 atctctccag aagaagcaga actgcggttt atagctttaa ttaaatagaa aacaatagtt
     6721 ttgattgtcg accatttcca atatgcgcta tactagcgtt gaggatggtg atccacatga
     6781 aatcattgat ttatgctatt ggggatgtcc atggcaaaca tgagcttttt gaaaaactta
     6841 ttcactatta tgatccaaag atccatcagc ttgttttatt aggggatctc aatgatcgag
     6901 gtcctcgtac aaaagaatgt tttttattag gaaagcaatt agtcgaagaa acaggagcca
     6961 tttacctccg gggaaaccat gaagaatatt ttttgcaatt tctgcaatca ccggaggact
     7021 ggtatgcgcc ttatatgaga aatggtggaa aagaaacgtt tgaaagcctg attcacccag
     7081 gggcatcgga ggaatactcc ccaactgaaa tctcgatgat gatccaatcg agatattcag
     7141 aactgattaa atttttagtc cagcgcccct tgtattatga atgggaaaat tatctttttg
     7201 tccatgccgg tgtggatttg acaaagaaag actggcgtaa gacaagtcta agagacttta
     7261 tttggataag ggaaccattt catgaaggga aaaacaatac aggtaagaca attgtatttg
     7321 gacatacgat tacaccaatg ctccatggag atatgcagac aacagacctc tggattcagg
     7381 atcataaaat cggaatggac ggtggggctg tattcggagg gtcattgcat ggggttgttt
     7441 ttgacaagga aggtattctt caagatattg aatatcagaa caacgaagga ccttggcatg
     7501 gaatctgact aatggaaaga atgaaggagc tgtgactttg ttgcggctct ttttattttt
     7561 atagagaata ttgtttcttt cagtagttta acccaggata aaaagtatga tagaataaag
     7621 aagcaatttt tagcagaaaa gaggcatttt aatgacggaa aatttgaacc agacgatcat
     7681 tcagcttgta caaaaagaac tttcagacta tcgtcctaag cagttgacta ctgtacttaa
     7741 tttgttaaat gaaggaaaca ctgttccttt tatcgcgcgt taccgaaaag aaatgactgg
     7801 aagtcttgat gaagtccaga ttcgtgaaat cgaagagcgg tatgcctatc ttgaaaactt
     7861 agaaaaacgt aaaactgaag tgattcggct aatcgatgaa caagggaaac tgactcccga
     7921 gttagaagca gaaatcaccc aagccgtgaa aatgcaacaa gtggaagatt tgtatcgtcc
     7981 atacaaacaa aaacgacgga caaaagcaac gattgctaaa gaaaaaggac ttgaaccact
     8041 cgcgaagtgg ctgatgcagc ttacagatgg agaggtgcaa tctgaagcag aaaaatatat
     8101 tgacaaagaa aaagaagtct cttctgccga agaagcgttg cacggtgctc atgagattat
     8161 tgctgaacaa gtcagcgata atgcaaaatt tcggacttgg attcggtctt atacgtataa
     8221 caaaggtatg tatgtcagca atgtcaagga tgagcaagca gatgaaaaag gcgtatatga
     8281 aatgtattat gactttgcag aacccgtcca taaaatggtt tctcatcgaa tattagctac
     8341 taatcgtgga gaaaaagaag atattctgaa agtattcttt caagtagatg aagcagcaat
     8401 tttagcttac ttggatcgcc aaatagtcaa aaatcctgcc tctccttctt cttcattcgt
     8461 cagagaagcc tatcaggata gttataaacg ctttattcag ccagcaatcg agcgagaact
     8521 gcgcaatgaa ttgactgaaa aggcagatga gcaagcaatc gctattttcg gcgaaaacct
     8581 ccgtaatctt ttgttgcaac cgccactaaa aggtaaagtt gtcctgggat ttgatccggc
     8641 ttatcgtact ggatgtaaac tagcagtcgt agatgcgaca gggaaagttt tagcaatcga
     8701 agttatttat cctcataaac ctgcagcgca agcaaaaaga gaagctgctg gaccagcttt
     8761 tatccaatta attaataaat atcaagtaga tatggtagct atcggaaatg gtacagccag
     8821 tcgggaatct gaactttttg tggctgagca gttgaaatcc gccgaccata aaacctatta
     8881 cgctatcgtc aacgaagcag gcgcttctgt ttactccgct agcgaaattg cacgaaaaga
     8941 atttccgcat ctacaagttg aagaaagaag tgctgtttct attgcccgtc gcctgcaaga
     9001 tcctttagct gaattagtca aaatcgatcc taaagctgtc ggagtaggcc agtaccaaca
     9061 tgacgtttct caaaaacgtt tggctgaaca attggatttt gttgtggaaa cagcggttaa
     9121 ccaagtaggt gtggatgtga atacagcaag cccgcagctc ttgcagcata tatcaggatt
     9181 gaataaaaca actgcccaaa acattgtcat ttatcgtgaa gaaaacggag aattcaccgc
     9241 acgaactcaa ttgaaaaaag tgccacgctt aggaccaaaa gcatatgaac aagcaatcgg
     9301 attcttgcga gtccctggtg ggaaaaacat tttggacaac acaggtatcc atcccgaaag
     9361 ttattctatt gcaaaagaaa tattaacttc agtccaactt tcagaaaaag agcttggtac
     9421 agaagaagca gtagaaaaat tgacacgatt atccgtagag aaactggctg aaagcctatc
     9481 agtcggagaa gaaacgttag ccgatatttt agctgggctg acacaacctg gacgagatat
     9541 gcgtgatgag atgccagcgc ccttattacg tacagatgta ttgagtatgg aagacttgaa
     9601 accagggatg gaattgactg gaacggtccg taatgtcatt gacttcggcg catttgttga
     9661 tatcggtgtg aagcaagacg gacttgtcca tatatcgaaa ctcagcaaaa aattcgtcaa
     9721 gcatcctaca gatgtcgtgt cagtaggaga tatcgtaaca gtttggatag aacaagtcga
     9781 tacgaaaaaa ggcagaatca gtttgacgat gctttcccct tatgaagagt aacaatgagg
     9841 agtaacaaaa attgacacaa gaagaactgc agcagttagt agaagagata tcattaaaaa
     9901 gcttccagct tcctttcaag caccaagcaa tgttcaatcc acgcttacgt acaactggcg
     9961 ggcgctatca tttaaacgat catcatttag attttaatgc gaagctattt gaagagtcag
    10021 acgaagagac gattgcagga atcatcaaac atgaactttg ccattatcat cttcatctag
    10081 ctggaagagg gtatcagcat aaagatatgg atttcaagta tcttttgaaa aaaacaggag
    10141 ggttgcgcta tgcaccagcc ttgacgaaga aacagataaa gatggagtat tatcgatgcc
    10201 aagattgttc tgccacgatc tgccgcaaga gaagaatcga tgtacgcaaa tatcgttgtg
    10261 gaagatgtca tggagaaatc aaatggattg agacaaaaag agaagacttt aatgaagcac
    10321 aaaaaatcag atgaaaggat agaaggcctt tggaaaaaag tgagcagagg aatctaaaag
    10381 actggaatca gctattaaat tttttagccg cacctgaaga gggaaatttt gaggaactga
    10441 cggcacctac acttatttta gctggaaact gtcttcctat cttagctgat aaagcagcac
    10501 aaatgtattt aaatggccaa gtagatcaat tgtttttggt cggaggggtg gggcatgcga
    10561 ctagaatcct ttatgagaat tttgaaaagc aaggatttca ttttgaagat ggcatgagtg
    10621 aaagtgaaat ctgtcgtcag tatctaaaag aagtctatga tttgcccgat aaggcttttc
    10681 tgatagaatc gaagtctaca aattctggag agaacgccac tttttcactg gagattcttc
    10741 attccttagg agctgtacca gaaaaaatct tattgatgaa tgatccgaca ctgcaacgca
    10801 ggactcgagc aacttttgag aaagtatggc aaaatgaaca aactattttc gcgaatgctg
    10861 tgccatttgt gccggaaatc cttcactttt ctgaagagat catctttaca gcaaaagaac
    10921 tgaatcatca atggcctaag gaatatttcc atgcgctagt attaggagaa atggaacgtc
    10981 tccatgacga tgagaacggc tatggtccga aaggaaaaga ttttattcct cacattgata
    11041 tatcagagga agtttggtct tcttacacca gaattaaaga gtcaataaaa acggattttt
    11101 ctcgtacttg accattttag caagcgaatc ctcctttcca aataaatttg gttgaaattt
    11161 tcaatcgttt ataagataat gaggagcgtg ttgaatgtaa tagctaaaag aatggacgct
    11221 agataagaga agaggattca aatggaaaat caaaataaat tgtatcattt tgttggtatc
    11281 aaaggttcag gaatgagctc cttggctctt gttttacatg aacaaggtct aaatgtccaa
    11341 ggatcagaca ttgaaaaata tttctttaca caaagagatt tagaaaaagc aaatatcacg
    11401 attctgccat ttaacgcaga taatgtaaaa ccagggatga cgatcattgc aggaaatgca
    11461 tttcctgatt cacatgaaga aatccagcga gcaaaagaat taggtttgga agtgattcgc
    11521 tatcacgact ttattggtca ttttatccaa aattatacga gtatcgctgt aacaggctct
    11581 cacggaaaaa caagtacgac tggactgctc tctcatgtat tgagtggtgt ccgtccaaca
    11641 agttatctaa ttggagatgg aacaggacat ggtgatccgc aagcggaatt cttttcattt
    11701 gaggcctgtg aatatcgccg tcatttcctt gcctattcac cagactatgc aatcatgacc
    11761 aatatcgatt ttgatcatcc ggattattat acaagtatcg acgatgtatt tacagctttc
    11821 caaacaatgg ctggacaagt gaaaaaagct atttttgcgt atggtgacga tgcttattta
    11881 cgcaaattaa aagcaaatgt gccgatttat tattatggtg tcacagaaaa cgatgatatc
    11941 caagcacgaa acatcgaacg cacaacttct ggttctgctt ttgatgtcta tcatggggat
    12001 gaatttgttg ggcacttcac tgtaccagca ttcggtaaac acaatatctt aaatgcttta
    12061 ggtgttattg cagtagctta tttcgaaaaa ctggatctga aagaagttgc agaagaaatg
    12121 ctgactttcc caggagtaaa acgtcgattc agtgaaaaga tcgtagcaga catgaccgta
    12181 gtcgatgatt atgcgcatca ccctgcagag atcaaagcaa cgattgatgg cgcacgccaa
    12241 aaatatccag ataaagaaat aattgcagtt ttccaacccc atacgtttac gcgtacgatt
    12301 gcattgatgg atgaatttgc agaagcactt gatttagctg ataaagttta cttatgcgat
    12361 atttttggtt cggctcggga agaacaagga aacgttaaga ttgaagacct tggagcgaaa
    12421 atcaaaaaag gtggagaagt catcaaagaa aataatgtat ctccattgtt agattatcat
    12481 gatgcagtag taatcttcat gggtgctgga gatgttcaaa aatttgaaca agcgtatgaa
    12541 aaacttttaa gcagcacgac gaaaaatgta ctgtaataca attactgcag tgtccttata
    12601 aaaatagaaa gagggggagt ggcaaaaatc ttgccaagcc cccttttttg caacttatga
    12661 taaacggtgt tcaaaagctt gcatctgcgg taataagccc aatcaaatca aaaacatgag
    12721 aagctgtttt ttcttggctg ttcttattat ttgatgcagg acagcttttt cacgacctct
    12781 gtaaactata ggaatctgtt aaatcaaggg agcagaactt gccttttacg ctacttttgg
    12841 tacaataatg tatgattaaa aataaactgg agggtttcta tcttgaacat aggtaagccg
    12901 agaaaactag gaaaaacaat aaatgaaatc gaggaaggcg actcgttatc cttaacagaa
    12961 tctattgaag ataaccagct tttgctgtat ttgggattga cgaatgatgc aaatcctttg
    13021 tatatccaac atgactttgc acaaaaaaca gaatatcaac agccaatcgt tccatcagtg
    13081 atgctgatgg ggatcatcac aagtgcaatt tcaaagcatc ttcctggacc aggatcacat
    13141 gttgtcaatt tttctgtcaa ttttattgaa ccggttttcc attatgaaac actgactttt
    13201 cagtttgagg tcataaaagt agataagatg aaggatgtgg tgacgatttc tgtagaagcg
    13261 gagaatacga acgaagatcg tgtgttggat gctgtcgtta tggtccaacc gccacaagtt
    13321 cctgaattgg aaatagcaat cgaaagtgaa ggtgaattga atgaataatc caagtacatc
    13381 aatgtcaggt atcaatcgtt tttatgcaaa agtttatgga tttttaggta tgggacttgc
    13441 ccttagtgca gtcacttctt acttggctct ttgggtattt cctttgcaat tagcaacttt
    13501 tatcaataat tttccactag gctttgtggg tctgtggatc gttgaactga ttttagtaat
    13561 ggttctaggc gtaaaagcac aaaagaatcc gagcttagca atcggtggat ttattgccta
    13621 ctctcttttg aatggcataa cattagcggt tactcttgca tactacgata ttggaacagt
    13681 aacgcaagct ttcttgacag ctactttgat gttcatgggg atgtctgttg tcggagtaac
    13741 aacaaaaaga gatcttagcg gggttggaag agcaggacag gcagctttaa tcggagtgat
    13801 cattgcacta gtcttgaaca tcttcatttt gagaagtagc gtagttgagc tgttcatctc
    13861 cctactgtta gtcgtcatct ttgcagggat tacagcttat gataaccaaa gcatccgtaa
    13921 tatctatgtc cagacaaacg gaacagcaca tagtggaatt gctgtattca tggcattgaa
    13981 gctgtatttg gactttatca acttgttctt ggcgtttctg agaatctttg gacggaacaa
    14041 ctaaaaaaga aaaaaattca tcagttgggg ctataccgaa ttatttggta tggtcccgct
    14101 ttttcttttc gcattccgca ggattctttg ttagaatgaa taagaacttt acagtaagga
    14161 gttaagaaga tgacaaaaaa taaattatta ctcgttgatg ggaacagtgt ggcttttcgc
    14221 gcattctttg cgcttcacaa ttctcttgaa cgtttcaaaa atagaaacgg tcttcataca
    14281 aatgcaatct atgcattcaa tacgatgttt gaaaatgtta tgcaaaaaga gcaaccgact
    14341 catgttctgg ttgcgttcga tgcaggaaaa acgacttttc ggacagaaat gtatgcggag
    14401 tacaaaggtg gaagatccaa aacacctggc gaattcaaag aacaaatgcc atatatacgt
    14461 gatttgttga caggactagg ggtacagtat tacgaattgc ccaattatga agcagatgat
    14521 attatcggta ctttggcaga aaaagtagac aaagatcaat ttgatgtcgt cgtgctttca
    14581 ggtgatcggg atttgactca attagctagt aaagaagtaa aagtggatat cactgtaaaa
    14641 ggtgtcagcg atatcgaaag ctatacacca gaacatgtag cggaaaaata tgatgggttg
    14701 acgccaaaac agattattga catgaaggga ctagccggag atgcctctga caatatcccc
    14761 ggtgtgacta aaatcggtga aaaaacagct atcaagctat tgaaacaata tggctcagtc
    14821 gaaggaattt atgaacatat cgatgaaatg aagcaaagca aaatgaaaga aaatctgata
    14881 aatgacaaag agcaggcttt cttatcgaaa aaattggcga ccatcaatac ttcagcgcct
    14941 gtggatgtct ctatcgactc attgaagtat gaagggaaaa atctcgataa actcgttccg
    15001 ttttataaag aaatggattt caatcagttc ctttctaaat tgaatatcgt agaagaagaa
    15061 gtaaaaatgg atgatatttt gtttgaagtc gttcatgaat tcaaagagga aatgtttact
    15121 acggatatgg cgttgtacgt ggaaatgatg ggcgacaatt atcatacaga ggaaatcgtc
    15181 ggagtagcgt gggggacaga aaagaaaatt tatgtcacga acgatctatc tgtattcgaa
    15241 aaccgagcgt tccattcttg gatcactgat ccgactcggc tgaaaaaagt atatgatgcg
    15301 aaacgaactt atgttgcttt aaaccgctat actggaaaaa cgaaaggaat cgattttgat
    15361 gtcttgctag gtgcatacct tctcgatacg aatgaaaaaa gcacagatat cgaaggaata
    15421 gctgctcatt acggatacaa cgatatccaa tcagatgaag ctgtatacgg caaaggggcg
    15481 aaaaaaggtt taccagaaga agaggaactc ttttttgctc atttagcgcg aaaagtagct
    15541 gcaatcaatg cgttgacacc aaaattgagc caagaactgg cagaaaaaaa ccaagaagat
    15601 cttttccgga aaatggaact gccattgtct atcattttgg cagagatgga aataagcggg
    15661 atcaaagtag atgcgactcg cttacaagaa atgaagggcg agttctctgc tcgcttaaga
    15721 gagatcgaac aaaaaatcta cgaagaagcc ggcgaagaat tcaatttgaa ttcaccaaag
    15781 caattaggag tgatcttatt tgaaaaaatg ggattacccg ttatcaaaaa aacaaaaact
    15841 ggatattcca cagccgtaga tgtactggaa cagcttcgtg aacaagcacc aatcgtagag
    15901 gatatcctca cctatcgtca aattgcaaaa attcaatcta cctatgtcga gggactgctg
    15961 aaagtgatcc aatcagatgg aaaagtgcat acccgttatg tccagacatt gacccaaaca
    16021 ggacgattaa gctctgttga tcctaatttg cagaacatcc cgattcgatt agatgaaggg
    16081 agaaagatac gtcaggcatt tgttcctaga gaaaaagact ggctgattta ttcttctgac
    16141 tattcacaaa tcgagttacg cgtacttgcg catatctcaa atgatgaaca tttgaaagct
    16201 gccttcctag aaggtcaaga tatccatgcc agcacagcga tgcgtgtatt tggaatcgaa
    16261 aaggcagagg atgtcacacc aaatatgcgt aggcaggcaa aagcagtgaa ttttggtatc
    16321 gtgtatggca tctctgatta cggactttca caaaatctag gaatcagccg aaaagcagca
    16381 cagcaataca tcgatacgta ctttgaaaaa tacccaggtg tcaaagaata tatggaaaga
    16441 atcgtacgcg aagcaaaaga ccaaggctac gtcgaaacgc tgtatcaccg ccgtcgatat
    16501 ctgccagaca tcaattcaag aaattacaat atccgttctt ttgcggaacg aacagctatc
    16561 aatacaccaa tccaaggaag tgctgctgat attttgaaaa tcgcgatgat cgaattggat
    16621 aaacgattaa aagaaacagg tcttcaagct acgatgctgt tacaagttca tgatgaactt
    16681 gtcttcgagg taccggaaaa agaacttgaa tcattagata aattggtgaa ggaagttatg
    16741 gaacaagctg tttctcttca tgtaccactg atcactgaca gcagttgggg aaaaacgtgg
    16801 tacgaagcaa aataaacatt gtttaagaca gagcaatata atgaaggtga aaagaaaaaa
    16861 tgccagaatt accagaagta gaaaccgttc gtaaaggatt ggaacggtta gtagtaggaa
    16921 aaacgattca aaaagttcag gtcttgtggc ccaagatcat cgagcaacca gagacaccca
    16981 tctttgaggc ttctcttgtt ggcgaaacca tccaatccat tggacgtaga gggaagtttt
    17041 taatttttca tctcgatcat tgtgaactga tctcacactt gcgaatggaa ggaaaatatc
    17101 aatttacgaa agaaaataca ccaatcgata aacatacgca tgtacttttc ttttttgagg
    17161 atggaagcca acttcgctac aacgatgtac gaaaatttgg tcgtatgaca atcgtcgaaa
    17221 aaggagcaag tgcaacttac cgaggaatca tgaaattggg acctgagcct ttgccagata
    17281 gcttcttatt agcagatttc gcaaatggac tgaaaaagtc ccacaaagca atcaagccat
    17341 tgctgctaga ccaacgtcta gtgactggtc taggtaatat ttatgtagat gaagcattat
    17401 gggaagcgaa gatccatcca gaacaaccgg cgaacacact gcgccccaaa gaggtagaac
    17461 aattacgtct atcgatcatt gatgttttgg atcgtgcaat agaagccgga ggtaccacga
    17521 ttcgcagtta tttgaatgca ttaggagaat caggcggatt tcaagtagca ctgcatgtct
    17581 atcagcaaac tggcaaacca tgtatacgtt gtggaacgcc gatcgtgaaa acaaaagtgg
    17641 cacaaagggg gacgcactat tgtccgaatt gtcaaaggct aaaaatcgca aagtaggatt
    17701 tgttttaggt ttgacgggtg gaatagctac cggtaaaagt acggcagcca aagtctttca
    17761 gtctcatgga tttcctttga tcgatggcga tgtgattgcc agagaaactg ttgagcctgg
    17821 aacgcttgct ctgaggaaaa ttgtatcagt gtttggccaa gaggtattgc agcctgatgg
    17881 gcaactagat cgagggaaac ttgggatgat tgtttttcca tcaaaggaaa aacggcaaaa
    17941 acttgatcag ctattagatc cgtttatccg aaaagcgata aaagatcaaa tcgcctctct
    18001 aagttccaat catcctttag taattgtaga tatcccattg ctctacgaag gtcattatga
    18061 ccactatatg gatgcagtag ctgtcgttta tactacgcca gaaactcagc ttcagcgttt
    18121 gatgaaacgt aatcaactga cgctagaaca agcgcagcag agaattagta gtcaacttcc
    18181 aatcgaagaa aaaaagcagc gtgcggatat tttgttcgac aacaatggaa caaaagaaaa
    18241 acttgtggga caaatcgaaa attggctttt tgctaatcgc tttctttgag tatctgtgtc
    18301 aattttgcta tcctgagtat atagaaagtg agggatagcg atgggatcat taaaaaatca
    18361 aaaggataaa gtcgtaggta acgcaaaaga aaaaatcggc gagatgctca ataaagaaaa
    18421 tctaatcgat gaaggtcgta cgcaatcaga aacagcagaa gaaaaagaaa agcagtacca
    18481 agcaagaatc gatgaaatag aagaccagca ggcagaagaa gaagcgaagc ggcaagattt
    18541 gactcaagaa cgaaaaaatc cgacacctga tcgtagtaaa acaccgctcc cagaaagtga
    18601 ccacattccc gatgagcaaa aaatcgagga acagcgaatg aaacactata caggaatcga
    18661 agagaagtta taaaatagta aaaaatcgag atgtgacttg aagcagatga gaaggtgttc
    18721 atctgcttca agttttttgt gctagatgac taaatcagct tttgtgtaag ttatttctca
    18781 gtaatcaagg tgatcagtat tggaaaattg aaatttagta aggacagggg acacaattac
    18841 gcatgaaagt agaaaaaatt gttcttatat ccgtgttttt tgggtaggac agtcgcgttt
    18901 gtgatttgtt tcgtgttata atacaactag ttgtaaagaa agacacatcc tttatataaa
    18961 atgcgatgca agaggtgaga caaaatgaga tgtcccagat gtcaacataa taattcacga
    19021 gtaatcgaca gccgtcaagc agatgatgga cgtgcgatcc gtcgccgtcg cgaatgtgag
    19081 aattgttcgt tccgattcac tacatttgaa cgaattgaag ctgctccgtt attagtaatc
    19141 aagaaaaacg gggaacgcga agagttcaat cgcgataaaa ttttaagagg actcatccgt
    19201 tcagcagaaa aaagacctgt agctatggaa caaatggaac aaatcgtcaa ccatgtcgaa
    19261 aaccgcgtac gcagtctcgg agaaaacgaa gtatcgacga atctaattgg tgaatatgtg
    19321 atggaagatt tagttgattt agacgaaatc gcttatattc gttttgcaag tgtttatcgc
    19381 cagttcaaag atatgtctgt tttcttaaaa gagttgcaag atatcgtgga taaagcaaaa
    19441 gaaaataata aagagaataa taaagaaaac aattaagaaa caaaaaggag gcatttcctt
    19501 ggaaaatgct tggaaagagc ttcaaccaaa aaatatttac caaggaagaa aagcggctcc
    19561 gctgagagaa gacagcaaga acgccttgct gtatctttat cagccactaa tcggcggaga
    19621 agccttgagt atctatttca ccctgttatc agaaatctct caagaatctg gcaaaggtcc
    19681 tgaagggatg catgcagatc tgcttagtag tctaggctgc ggtatcccgc aattttatga
    19741 tgcaagaaaa aagctagaag gaatcggctt actggatgtt tattttaaag aagatagtga
    19801 tctgggacct cgttttattt atgagctgtt agaaccactc attcctaaag catttttcaa
    19861 agactctgtt ctggcttttc ttcttctgga aaaagttggt gaacgacgat tccagaagct
    19921 tacccaattc tttgaaccta aaaggcatac agcagaggga tatcaaaaaa tcacaaaaaa
    19981 atttttagat gtttatcgct tcaatgaagc agtgtacgct gctaatcaag aaaacatcga
    20041 atcgctgaat aaatcttttg aagaatctga atcagtagca ttacttgaag cagattcgac
    20101 attcgattgg cagtttttga atgattggct ggcaaaaaaa catattgcaa gtcttgatga
    20161 tccaatcatc aaacaagtcg acatgtatca tcagctttac ggtttcgatg aattgacact
    20221 tggcgaactg atcagtcaat cttatgactt tagtgaacaa aaagtatctg aaaaagaact
    20281 gcaaaaagcc attttggcaa tgcagagacg tccggtgaag caaacaaaag aaacagttgc
    20341 ggtttctgaa aacagtaatg aaccctcgtc aaacgaacat ttgacaaacg cagcgcttca
    20401 gctgataaaa gaagcacaga gcattccacc gatgaaatat ctggaagcaa tcaagtcaga
    20461 aaaaggcgga tatgtttcta aacaagaaaa ctggttactg caagatcttg tttctcaatc
    20521 aggtctttcc agtagcgtga tcaatgtttt gctgaattat gtactagtga tcaagaataa
    20581 tgctagctta aatgccagtt atgtaaatac agtggcaaat gaatgggcac aaaagaaaat
    20641 cgccacagca gaagaagcga tccagcacat tcggcaaata agtaatcaag caaaacaatc
    20701 aaaacaaaag aagcaaacaa attatcaaac tggcaagcga aatgttcgac gggaaaagct
    20761 tcctgattgg gtcaaccagc caaaagatga aaaacagatc agcagcgaga aaaaagcaga
    20821 gattgaccgc cgttttaaag aatacctggc aaaaaaggag ggtgatatct agtggaagac
    20881 gtaggcaaag aattgaagag gatcatcagc aaacgcaatt atcaagaaaa gtatgctgaa
    20941 atgatcgctg aagtcttaaa tgatgcggat gttcaagctt ttctaagcgc acaccaagaa
    21001 gaattgacac aagcggatat tgaaaaaagt tacgctaaat tgtatgaatt cgtgcaagaa
    21061 aagcgtaaat atcaaaaaaa cgatccaacg atgatcgctc ccggctatga gccgaagctg
    21121 acgttgaatt ttcattatgt ggatgtcact tatgtaccaa cggaagcatt gttagcacgg
    21181 caaagacaag aagaaatcag aagccgagta aaagcaatgg atatgccaaa agatattcaa
    21241 tcggcttctt ttgaaaactt tgaacgtaca gatggacgag gatatgcgag cttagaagcc
    21301 ctagattttg tagagaaata tactgctgat ccaaaagggt atcataaagg actgtatcta
    21361 gtaggaagtt ttggtattgg aaaaacatat ttattaggag caacagcgag agaattagcg
    21421 attgctggat tcaccacgac gctagtccat tttccgacat ttgccgttga gatgaaacag
    21481 gcaatcggaa aagatcaagt ggcagaaaag ttagatgcag tgaaacgttc acctattttg
    21541 atgatcgatg acattggtgc agatgcgatg agtagttgga tacgagacga agtattcggt
    21601 gttatccttc aatatcggat gcaggaacag ttaccaacct tcttctcatc gaattttaca
    21661 atggatgaat tggaacagca tttgtccgtc actcaacgtg gagatgaaga gccgctaaaa
    21721 gcgaaacgga taatggaacg catacgttat ttgacaaaag aaatcgaaat gactggtcgc
    21781 aaccgacgca atggttgaac agttgacaag tccaactgac atttgaccta gtagtaaaag
    21841 caggtcagcg aatcattata tggacagaaa tgaccggaga caatcaccgc aacaagtaat
    21901 gcagaaactt aaacggagga ttttttatga gggctatttt ttctagtata tctgtagttg
    21961 gtctattttt tcttttagca ggctgcggta gcgcagatag caacacaggt tctgttgatt
    22021 cgattgatgc gggtcaaaca gtaaaaacaa gcagtacgaa aacggagaga caaagcatat
    22081 ttgtcggaac gattgaaagt atcactcaag tagaagcaga agaagaaacg cttcagttga
    22141 aactcgtcga tgtcatggcc aaagaagatc cagcgaatat gggaacgtca ttcagcaatg
    22201 acggagtgat cttaaatgct actgctgaaa aaataagcgt ggacataaat gaattgaaaa
    22261 tcggtgacaa agtccaattt acattagttg agatgccgat catgacgatg tctatcccac
    22321 cgcaagttcc tggagattcg attattgatg tttcattgaa caaataacca ataacatttt
    22381 ttcaaaaata agaaaatgtt gttttataga acgaaaaggt atatttttta aggagatatg
    22441 ggaatttttt tattcctagt ctccttattt tatttggaaa atgctataat tatctagaag
    22501 gagtgaagct atgaaccatt tagatttatc tcagcgctta gccgcagtgg gtgactttgt
    22561 ccctgaatcc gctcgtttag cagacattgg atcagatcac gcttacttac ccgttgcatt
    22621 gatattaaga ggaaaaatcg aatatgcagt tgctggtgaa gtcgttcaag gtccttatga
    22681 atcagcacgt aagcaagtca aaaaagatgg attagaagac cgtatcaccg tacgccttgc
    22741 aaatggtcta gatgctatcc agccacaaga tggtattaca gctgtcacga ttgcgggtat
    22801 gggaggttca ctgatccgag atatcctaga agctggcaag ttgaatcaac gcttaagcgg
    22861 acaagaacgt ttgatccttc agccaaacat cggagagcgt acattacgat cttggctgca
    22921 agaaaatcag tataaaatcc aatcagaaaa aatacttgaa gaaaataaaa aaatatatga
    22981 aatcattgta gccgaaaaag aacaaaaacc agttacttac aatgaaatgg agctgatgtt
    23041 cgggccattt ctgcttgaag agaaaagtga gatcttcaaa tcaaaatgga caagagagct
    23101 tcaacaaaga aaagctatcc gtgaacagct caaacaagca gctaatcaag aggcacgtca
    23161 aaaacaaatc gagcaggaaa tccaaatgat tgaggaggtt ttaaagccat gacaatcagc
    23221 ggacgcactt tcatccaaaa gtttgaaagc tattgtcctc agtggctagc tgaagaagga
    23281 gacccagtcg gactgcatat cggaacattg gataagccaa tccaacgagt aatgatgaca
    23341 ttagacgttc gtccagaagt ggtcgaagaa gcaatcaaga aaaaaatcga tttgctgatt
    23401 gctaaacatc ctccaatctt tcgtcctgta aaacggctgg ttactgatca gccacaagaa
    23461 aaaatgtatg cagatttgtt gaaacatgat attgccgtct atgcagcaca cacgaacatg
    23521 gatattattt gggatggact gaatgactgg ttttgtgaac ttctcggtat tgaagtggag
    23581 agttatttag taaagactca tgagattcat tataaaaaac tggccgttta tgtacctgtg
    23641 gatcatgcac agaaaatgcg tgaagtatta gctgcagctg gtgctggtac acaaggtgat
    23701 tataccggta caagttttac ctcgattgga cacggtcgat ttactccgga agctggtgca
    23761 caaccagcga ttgggaaagt agggaagaca gaacaagttc aagaggcaaa agttgaggtt
    23821 attttgccgg aaactatcga aaaacaagtg attcaagcca tgcggtcagc tcatccttat
    23881 gaggaacctg cttatgatct tttcgctata gatgaaccag tcgaaatgtt tgggttagga
    23941 cgagtaggtg aattgccgca agaaatttct atagaggcat ttgtagaaca agtaaaagaa
    24001 gcgttccaac tagatggact gagaatagtc cagccaaaaa atgcaaaatc ctctgtgaag
    24061 cgcattgcga tttgcggtgg aagcggtgaa aaattttatc cgcaagcaat cgcccaaaga
    24121 gcagatgtct atatcacggg agatatctat tatcatacgg ctcatgatat gcaaagcgct
    24181 gggttgattg caattgatcc cggtcattat attgaatcgt tatgcaagca acgatttatc
    24241 gagaaatttg aatcttggaa acaagaagaa aattgggata ttaacttttt cgtttctgaa
    24301 acaaatacaa acccatttca atttaattaa aaggagagaa tactatgtac gaaaatcttt
    24361 tgccacgttt tttacgatat gtcaaaacag aaacacgctc cgatgcaaca agcaaaacga
    24421 ctccatcgac tcagacacaa gttgcttttg cgcaagaatt gaaaaaagaa ttagaagagt
    24481 taggcttagc tgatgttttc tataatgaag ataacggatt cgttatggga acattaccaa
    24541 gcaatgttga tcatgaagtg cgttccattg gatttatttc ccacatggat acagcagatt
    24601 ttaatgcagt aggagtcaat ccacaaatca ttgaaaacta tgatggcgag tctacgatcg
    24661 tactagatgc ggaagaacgc tatacattga atacaaaaga ttttccaaat ttgaaaaatt
    24721 atgaaggcca cacattgatc acaacagatg gcagtacatt actgggagca gatgacaaat
    24781 cagggattgc ggaaatcatg actgcaatgg agatcttgat caataatcct tctatccctc
    24841 acggtaccat caaagtggct tttggtcctg atgaagaaat cggtgtcgga gcggataaat
    24901 tcgatgttga gcaattcgat gtagattttg cttatacagt cgatggtggt cctttaggtg
    24961 agttgcagta tgaaacgttt agtgctgctc aagcaaatat tacgatccaa gggaaaaatg
    25021 tacatccagg gacggcaaaa gatacaatga tcaatgctct acagttagca atcgattttc
    25081 ataatcagct tccagccgat gaagttccag aaaaaacgga aggatacgaa ggtttcttcc
    25141 atttgatggc attggatggg aatcctgaag aagcttctat gagttatatc attcgcgatc
    25201 atgatcgtga aaaattcgaa gcacgcaaag cacaaatcac acatatccaa gaagagttga
    25261 atcagcgatt tgatcaagaa cgaatcaaag tagatatgta tgatcaatat tacaacatga
    25321 aagaaatcat tgaaaaagat atgagcatcg tggaattagc gaaacaagca atgatcgagc
    25381 ttggtatctc tccagtgatc gaaccagttc gtggaggaac agatggttca aaaatctctt
    25441 acatgggtat tccgacgcca aatctatttg ccggaggaga aaatatgcac ggacgattcg
    25501 aatttgtttc tttacaagta atggaaaaag caacagacgt gattgtaaaa attgcagaat
    25561 tgaacacaaa gagttaaaat cgaaaaagaa cagcttatga caaggcgttc gtcccaatga
    25621 tggaatgtca tctgtctaag gctaaagggc aggacaatgg aattggttgt cccgccctct
    25681 tttttgaaga gtaaatagga ggtaaagaat ggaaaagctg ctagtttttt ataacaaaag
    25741 ttcagggaaa gatgagggtg agaaattagc acgctggttt caagattatg taaaagaaaa
    25801 gcagccagaa ttatatgtag aactagctga aactggccca tcgattgatg atgcggaatt
    25861 agtcaagaaa gcagaagcca tagaagctga tactttagta attataggtg gagacggcac
    25921 gattcatcat atcgtccaag cgttccaaaa aacattatct caataccatg tcggattact
    25981 tccaggtgga actgtcaaca accttgcacg tgttcttgag attcccctca aaaaagaaga
    26041 agctgctgac gtgatcctgg aaggcaatgt acggaaaata gactatggtc aagtcaatga
    26101 tgaagtcatt atctctacac tgacaatcgg aatcttagca gatacagcag tttggatcag
    26161 tcaagaagaa aaacagcgtt acggctcgtg gatctttatc aagaagtttt ttaagttatt
    26221 gctgaagaag aaaaaatatc atttggattt agaaacagac gaggagcatt ggaaaggaaa
    26281 atcccaactg ttgacagtca cgatgtctaa ctccgtgggc ggttttacaa attttgacga
    26341 ctcagcgacc ctagatgatg gaaaatttca tgtaacgatc gtaccttcct tagatatttt
    26401 ccgatttatt ttttatttgc ctagaatcat gagaggcaaa ttctatacga tccccggcat
    26461 tacttatttc acagccaaac gcatgaaaat ccaagcgcag gaaaaatctg tgggcactcg
    26521 aacggatgga gacacgacag atgatcttcc agttgaatta tctgttgtat caaaaggatt
    26581 aacagtttat tcaccaaaga aagaagaaaa agcttagaaa tagtagttaa cagaaagttg
    26641 gaagaagagt aatggatgta ttgttacaca atcctttgtg gttttacctt ttcattgtga
    26701 atttttactt attttgtctg atggggtatg ataaacatca ggctagaact agcggctggc
    26761 gtgttcctga gaataatttg atatttgtca gcctgttagg cggtggtttg ggaggattga
    26821 tctctcgaaa agttttccat cataaaactc ggaaaaagaa gtttactata ggattcgctg
    26881 caggtgtact tgttgatttg attctcattt attttttcca ttaaggagca ggagatgtcg
    26941 tctaaaggaa cgaaaactaa aatcattctg aatctgcttt tgctgattgt gatttttgcc
    27001 attatttatt atttaatcaa tcaatcattt gcagatatct ttgccgaatt gctgtctacc
    27061 agtgttgttg ttttggcagc ggctgttata ttagggacag tttaccaaat cgctgaaggg
    27121 cgctcaatca aagaaattgc tgaacctttc gcgccgaatt ttacaacgaa agatgggttc
    27181 ttgacctcta gctatattgc gttttaccga atcgtttcgt ttggtacagg aacgctgatc
    27241 tcggagattt atttttataa caaaaaagga atgaagctat cccagggagc aggggtgaca
    27301 gcactccata tgattatgta caaactggca gtgatcactt atgcattagc tggcttggtc
    27361 atccaattct ctctttttta ttctaatggt ccatcaatga tctggtttat catcgctggt
    27421 atccttttga cgatcgtgat tgttgccttt ttattggcga tgtccgtcag cttgaatgtt
    27481 caaatatttt ttgtcagctt atcgaataag ttatttaaaa gtgagcgttt gcggaaatgg
    27541 gtggatacat gcaatcagca gatctactct ttaagggaaa cggtccaaac cattatccaa
    27601 gacaggactg cgctattgcg gatttatcta tggaatatgt taaaacttgt attttggtat
    27661 gtacttcctt atttgattct gattgaaaac catcctaaaa tagattttct actgaccatt
    27721 tcgtttatca gctttgcagt agtgctttca ggggttattc ctacgcctgc tggtattggt
    27781 tcctttgaat ttgtctattt gttattattc agaccagttg ttggaacagt tgacgcagtt
    27841 tcttcgcttt tactgtatcg tttcagttcc tttatcttgc catttctcta tggatttgtg
    27901 tatgtgattg ctgaacggcg aagagtgatc aaagaagaaa taatagaagt aaaaaaagag
    27961 aataaagaaa atcaagggta ggtcttttat aagatctacc ctttttatca aatcaaagaa
    28021 aaacgttatg gataagataa ttttacgttt gtttggctct ttttttatat acatacaatc
    28081 aattttgttt ttttaagatt tatataagtc gagaaaaaaa gtcaaaaaac tatgtgtata
    28141 ttttgaccaa aattgaccaa agcgatataa tagtttttgt aaagatcaaa gaaataagtg
    28201 atcttacctt taattaggag ggtatacctg tatgaatatt gagaaaatga ctacgacgct
    28261 tcaagaagcg attgctgaag ctcaacagat tgcggtcact cgaaaacatc aagatatcga
    28321 cattgcccat gtatggaaaa tctttttaca atctaatcat tttggtcgaa atttctatac
    28381 ggatgccgga ttggatgtcg aatcttttga acatgaaatt gatcgtctac tggatgagta
    28441 tccagttgtt tctggtggaa acgtccaata tggacaaaac ctcagccaga atttctttcg
    28501 attattgaat gaagcagacc aaatacgtga aagcttcggc gacgagtttc tttcaacgga
    28561 agtcgttatt ttagcgttga tgaaattaaa aaattatccg ttaactgttt atttgaataa
    28621 aaacggatta tctgaaaaag aattgcgtaa aaacatcgaa gagatgagag gaggagaaag
    28681 agtgacctca caaaaccaag aagaacaata caaagcatta gaaaaatacg gcgttgattt
    28741 agtacagcaa gtcaaaagcg gaaaaatgga tccaatcatc ggtcgtgatg aagaaatccg
    28801 tgatgtcatc cgtattcttt cacgaaaaac aaaaaataat cctgttctga taggagaacc
    28861 cggtgtaggg aaaacggcaa tcgttgaagg gcttgctcaa cgtatcgtac gcaaagatgt
    28921 gcctgaaaat ttaaaagata aaacgatttt ttcattagat atgggtgcat tgatcgctgg
    28981 agcaaaattc cgtggtgaat ttgaagaaag actaaaagca gtcttgaaag aagtcaaaaa
    29041 aagtgatgga cgtatcctct tgttcatcga tgaaatccac aatatcgtag gggcaggaaa
    29101 aacagaagga agtatggatg ccggaaatct gttgaaacca atgcttgctc gcggtgaatt
    29161 acatttgatc ggtgccacaa ctttagatga gtaccgtcaa tatatggaaa aagacaaagc
    29221 gttggaacgt cgctttcaaa aggtattagt caaagaacca acagtggaag atacgatcag
    29281 tattctgcgt ggattaaaag aacgttttga gatccaccat ggtgttaaca tccatgacaa
    29341 cgcattagta gcagcagcta ctttatctga ccgttatatt actgatcggt tcttaccgga
    29401 caaagcaatc gatttgatcg atgaagccag tgcaacgatt cgggtagaaa tgaactctat
    29461 gccaacggaa ttagatcaag ttactagacg tttgatgcag ttggaaatcg aagaagctgc
    29521 actgaaaaaa gaatcagatg atgcaagtaa aaaacgcttg aaaaatttgc aagaagaatt
    29581 agctgaactt cgtgaagaag caaatgcgat gaagatgcag tgggaaacag aaaaagaaga
    29641 agtcaattct gtttcagcaa aacgtgctga aatcgataaa gcaaaacacg aattagaaga
    29701 tgcagaaaat aactatgatt tagaacgcgc tgctgtattg cgtcatggta cgatacctca
    29761 attagaaaaa gaattgaagg aattagaagc taaagcaaaa gacagcgaga ttaagatggt
    29821 tcaagagtcc gttaccgaaa atgagattgc tcaagtcgtt ggcagattaa cgggtatccc
    29881 cgtaacgaaa cttgtagaag gtgaacgaga aaaactaatt aaattgaatg aaacacttca
    29941 taaacgagtg attggtcaag atgaagcagt tgatgcggtc agtgacgcgg taattcgttc
    30001 acgggctgga ttgcaagacc caaatcgtcc tctaggttct ttccttttcc taggaccaac
    30061 tggtgtcgga aaaaccgaat tagcgaaagc gttagctgag aacttgtttg actcagaaga
    30121 tcatatggta cggatcgata tgagtgaata tatggaaaaa catgctgtgt ctcgtctagt
    30181 cggagctcct ccaggctatg taggttacga agaaggtggt caattaacgg aagctgtcag
    30241 acgaaatcct tatacaatcg tcttgttgga tgaaatcgaa aaagcgcatc ccgatgtctt
    30301 caatatcttg ttacaagtcc tagatgacgg acgcttgact gattcaaaag ggcgagtagt
    30361 agatttcaaa aatacggttc tgatcatgac cagcaatatc ggttcacagt tactgctgga
    30421 aggcgtgact gctgatggaa cgattccaga agcagtggct gaacaagtca acacgctatt
    30481 acgtggaaac ttcaaaccag aattcttgaa ccgaatcgac gacacgattt tatttacacc
    30541 attgagccta gacaacgtga aaggaatcgt tgacaaaatg gtggcgcaat tggcacaacg
    30601 tctggaacat caagaaatcc tgctgacaat cagtgatgaa gccaaaacgt ggattgctga
    30661 aaatgcttat gaaccagcat atggtgcaag accattgaaa cgatttatca ctaaggaagt
    30721 agaaacacca ctagctaaag aaatcgttgc aggacatgtt atgcctaaat caaaagtaac
    30781 gattacttta cttgatggac aacttcattt caaaacagaa gagctagaag aaatcgtcta
    30841 atcttccatt gagactataa aagaaaaaag gggtctgaca caactagtgt cagacctcct
    30901 tttttcaaat aaacagtgtt ccagaagtag tttcttcgaa aaataagccg aaatttcttg
    30961 aagctgcctg cttctgtcac aacctctttt attaggtatt tttcactttt tctaatttat
    31021 aactttcaaa gtaacgctca tcttttttac tttgagacca tgcaaacaat cccattaagg
    31081 cagcacctat cgtatttgtc agcaagtcgc ccatcgtatc catcaatgct gctcgtccga
    31141 caaacagtgt gccatcagaa gcagcaaaac gttgaagatt catgcctaag aactggtcgc
    31201 ataagaattc ccagaactcc caaaacacgc cgcaaagtcc agcaaacgca aagccgaata
    31261 agaggaacaa ccaaggactg gtttttgata tctcagcatc tttcatcaag taaccaatca
    31321 atccatagcc aagagctgtc aggaccattg gactcaccgc atgtaagatt ttgtcccaaa
    31381 aagagatgat actgatcaaa tgcatccctg tgcctaaaaa gacagagatg aacaagaaaa
    31441 accagtaaaa caaaacgatt gctgctggta ttttaagttt gaaaactttc aaaatgactt
    31501 gtggcatgaa aatcaatgca atccctaata aagattccac gataaatgtg attccttgag
    31561 aagtcgaata tttttgtgtg ataaattcat aaaaattata gcagacaacg aatacaccaa
    31621 aagcaagtaa agctatttgc acctttttga tttctttaat cactgaaagt ccctccatct
    31681 aaaaatcttt ttacttctga aagcaactca atttctgaag aaaccacttt tccgttcaat
    31741 ttgatcaaac ctattgtata taagttcagg tagtgaaatt gattttcagc tgtttcttgt
    31801 agtgcagcga tttttttcgg gtgggttccg ccttgctgtc gagtatcagt atataagccg
    31861 atcactggga tgcctttcgc ataagccaca ccgatttccg atgctacccc ggcatcgatc
    31921 gtcagcccat ctagtaaagc aatcattaaa tcactagcca acactttttc tgtgtcagca
    31981 agagcaatca ttcgactgtc tgcataagca gttttgtcat tgattgcttc attttcttga
    32041 ggaagataga tcgaaagctc agaagagagc tctctgattt ctttaactaa ctgttggtta
    32101 tataacaaat cacttttagc aaacattggt gcggcaaaat aaattttcat ctaaacatca
    32161 tcctttttat caaaaataat cgctaaagta agcttccatg tttggaatga tacgctctag
    32221 tgacttcagt gtttcttcat ctgtttctaa ccgttcgatt cttgccaaat aagaagcacg
    32281 gcgataattc atcatcaagc aagtcaatgt ttggatatcc agtcgaacgg attttcctat
    32341 aggttcattt aagacacgga cttgtccctg ttcatcccat gttaaagcga agacaccatt
    32401 attccaagga gcgacaggat cttcaatgat gaaatgaaaa gcatcagctg ttccaacaaa
    32461 cggaaaccgt tgaaggaatt cttttacatc aacgatccga gccataaaat atggttcgat
    32521 ctgctctttg atctgactat cttcaatcaa aaatgctaaa ggctcatttt tataaatatc
    32581 tccatgtacc cagtagatca tggaaaaatg tgcggaaata aagttccata agccatttcg
    32641 agcttcttga tcaagataga acatttcttt tacgtgaaat atttcttcag ctacccagta
    32701 gaacagaaca cctcttggtt catggttgct atcgtagtag acagcggctg tgcgctcatc
    32761 ttcattttcg aaacgccaat actcttccca atgaaaatca ctgcggaata aagcaccatg
    32821 gttttgttgg gcaaatttgt cataaacgga aaatacgtct ggatgatcaa ctggtaatcg
    32881 ctcaaccata ccacttactg ggatttgttt aggtagctgt gtatctctga ttttaaagga
    32941 aagtttatca gacatgattt cccatccttt acgccgatag taggggatac tgtaaggata
    33001 caaataagag atccattgct tattcttgcg catattatcc aatgcaacga taattagatc
    33061 ttgcatcaat ccatggttag catattctgg ataagttcca actccagtca caccacccat
    33121 tttataacgt gcaccgtgaa tattgacttc acaaggatag atagcgattt gagagatcag
    33181 tttattttcg tggaaccatc cgaacacttt cgatacttcc aaaatcggtt ttttggaacg
    33241 gataaaagct cgtttattct caaacccgct ttcctcgatg tctgcttcag tcacttggaa
    33301 cacataggaa agcaattcat tgaattgatc gatatgttct tgagtgaccg gttttaattc
    33361 aagattttct ctaaaatctt tttgatccat tttttcatct ccatttatta gcttgataac
    33421 agtatagcaa aattctacaa aaaaaacttc tatagctgac taattaaaaa ctctttaaat
    33481 tactgctttt cgttggtata atggaaagtg ctgattatat gaaaagagga gacgctatgg
    33541 atataaagaa aatgaaagaa cgccaagagc agattcgtaa tttttcgatc atcgctcata
    33601 tcgaccatgg gaaatctaca ttggccgatc gtattttaga aatgacgcac actgtgacgt
    33661 ctcgcgaaat gcaagatcag ctgttagact cgatggattt ggaacgtgaa cgtggaatca
    33721 cgattaaatt aaatgctgta gaactcaatt acacagcgaa aaatggagaa acatatatct
    33781 ttcacttgat tgacacccca gggcacgtcg acttcaccta tgaagtctct cgaagccttg
    33841 ctgcctgtga gggtgccgtt ttagtggtcg atgcagcaca aggaatcgaa gcacaaacat
    33901 tggccaatgt ttatttagcg ttagacaatg atttggagat cctgccagtc atcaataaaa
    33961 tcgacctacc cgcagcagat cccgaacgtg tccgtcaaga aatcgaagac gtaatcggaa
    34021 tcgatgctag tgaagctgta ttagccagtg ccaaatcagg gattgggatt gaagatattc
    34081 tcgagcagat cgtggaatat gttcctgcac caagtggaga cttagatgca ccgctaaaag
    34141 ctttgatctt tgactctatt tatgatagtt accgcggtgt tgttctaaat gtccggatca
    34201 cggacggtat ggtcaaaccc ggagacaaaa tcaaactgat gagtaatggc aaaacatttg
    34261 atgttacaga ggtcggggtc ttctcgccaa aagcagttgc tagagacttt ttgatggtcg
    34321 gtgatgtcgg ttatatcaca gcaagtatca aaactgtcca agatacacga gtaggggata
    34381 cagtaacgtt agcagacaat cctgccgctg aagctttacc aggttaccga aaaatgaatc
    34441 cgatggttta ttgtggtctt tatccaattg atacttctcg ttacaacgat ttgcgggaag
    34501 ctttagaaaa actgcaattg aatgatgctg ctttacaatt cgaaccagaa acctctcaag
    34561 cattaggttt tgggttccgc tgtggattcc tcggacttct tcatatggat gtcgtccaag
    34621 aacgcttgga acgggaattc aatctagaat tgatcacgac tgcaccatcc gttatttacc
    34681 atgttaataa aacggatggt acgactgcta cagtagataa cccggcagat tttcctgaac
    34741 ctgtaacgat tcaggatgta gaagaaccat tcgtcaaagc acagatcatg gtcccaaatg
    34801 attttgtcgg tgcagtaatg gaactttctc aacgaaaacg gggagaattc attacgatgg
    34861 attatttaga tgattaccga gtaaatgtcg tctataatat tccgctttct gaaattgtct
    34921 ttgacttttt cgacaaattg aaatcaagca cgaaaggcta tgcctcattg gattatgaaa
    34981 tgtctggtta tcaaaaaagc aagttagtga agatggacat cttgctgaat ggagaaaaag
    35041 tcgatgcatt aagctttatc gttcaccgag actttgccta tgaacgtgga aaagcaatcg
    35101 ttgaaaaact gaaaaaacta atcccacgcc aacaatttga ggtgccaatc caagcagcta
    35161 ttggacaaaa aatcgttgcc cgttcagaca tcaaagcctt gcgtaaaaac gtcttggcta
    35221 aatgttatgg tggagatgtt tctcgtaaac gtaaactctt agagaaacaa aaagaaggga
    35281 agaagcggat gaaacaaatc ggctccgtcg aagttccgca agaagccttc atggccgttc
    35341 tgaaaatgga cgaggacgaa ccgaagaaat agcaagggtt tgtttgaaaa cagtgtaaaa
    35401 aataaaccat gtttacatgg atttgccacc ggtttgccac cggtggtttt
//
LOCUS       DAXWOO010000010        32971 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.010, whole genome shotgun sequence.
ACCESSION   DAXWOO010000010 DAXWOO010000000
VERSION     DAXWOO010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32971
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.010"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            212..1123
                     /locus_tag="ACGE70_000396"
     CDS             212..1123
                     /locus_tag="ACGE70_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFF8692064.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(1379..2296)
                     /gene="rnhC"
                     /locus_tag="ACGE70_000397"
     CDS             complement(1379..2296)
                     /gene="rnhC"
                     /locus_tag="ACGE70_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFF8692065.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            2498..2935
                     /gene="zapA"
                     /locus_tag="ACGE70_000398"
     CDS             2498..2935
                     /gene="zapA"
                     /locus_tag="ACGE70_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFF8692066.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            2993..3540
                     /locus_tag="ACGE70_000399"
                     /pseudo
     CDS             2993..3540
                     /locus_tag="ACGE70_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
     gene            3655..6015
                     /locus_tag="ACGE70_000400"
     CDS             3655..6015
                     /locus_tag="ACGE70_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFF8692067.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            6102..6416
                     /gene="trxA"
                     /locus_tag="ACGE70_000401"
     CDS             6102..6416
                     /gene="trxA"
                     /locus_tag="ACGE70_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFF8692068.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            6598..8376
                     /gene="uvrC"
                     /locus_tag="ACGE70_000402"
     CDS             6598..8376
                     /gene="uvrC"
                     /locus_tag="ACGE70_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFF8692069.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            8521..9420
                     /locus_tag="ACGE70_000403"
     CDS             8521..9420
                     /locus_tag="ACGE70_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8692070.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            9413..10675
                     /locus_tag="ACGE70_000404"
     CDS             9413..10675
                     /locus_tag="ACGE70_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8692071.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            10838..12736
                     /locus_tag="ACGE70_000405"
     CDS             10838..12736
                     /locus_tag="ACGE70_000405"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFF8692072.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(13035..13754)
                     /locus_tag="ACGE70_000406"
     CDS             complement(13035..13754)
                     /locus_tag="ACGE70_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFF8692073.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     misc_feature    13944..13991
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            14091..14423
                     /locus_tag="ACGE70_000407"
     CDS             14091..14423
                     /locus_tag="ACGE70_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692074.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            14410..16413
                     /locus_tag="ACGE70_000408"
     CDS             14410..16413
                     /locus_tag="ACGE70_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFF8692075.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            16432..16902
                     /locus_tag="ACGE70_000409"
     CDS             16432..16902
                     /locus_tag="ACGE70_000409"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFF8692076.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            16923..17507
                     /locus_tag="ACGE70_000410"
     CDS             16923..17507
                     /locus_tag="ACGE70_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HFF8692077.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            17522..18508
                     /locus_tag="ACGE70_000411"
     CDS             17522..18508
                     /locus_tag="ACGE70_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFF8692078.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            18498..18809
                     /locus_tag="ACGE70_000412"
     CDS             18498..18809
                     /locus_tag="ACGE70_000412"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFF8692079.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            18831..20612
                     /locus_tag="ACGE70_000413"
     CDS             18831..20612
                     /locus_tag="ACGE70_000413"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFF8692080.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            20605..21981
                     /locus_tag="ACGE70_000414"
     CDS             20605..21981
                     /locus_tag="ACGE70_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFF8692081.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            21988..22623
                     /locus_tag="ACGE70_000415"
     CDS             21988..22623
                     /locus_tag="ACGE70_000415"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFF8692082.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            22653..24008
                     /locus_tag="ACGE70_000416"
     CDS             22653..24008
                     /locus_tag="ACGE70_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFF8692083.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(24200..25594)
                     /locus_tag="ACGE70_000417"
     CDS             complement(24200..25594)
                     /locus_tag="ACGE70_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFF8692084.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            25686..26657
                     /locus_tag="ACGE70_000418"
     CDS             25686..26657
                     /locus_tag="ACGE70_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFF8692085.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            26654..27436
                     /locus_tag="ACGE70_000419"
     CDS             26654..27436
                     /locus_tag="ACGE70_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFF8692086.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(27605..27976)
                     /locus_tag="ACGE70_000420"
     CDS             complement(27605..27976)
                     /locus_tag="ACGE70_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFF8692087.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            28110..28553
                     /locus_tag="ACGE70_000421"
     CDS             28110..28553
                     /locus_tag="ACGE70_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFF8692088.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(28596..29405)
                     /gene="yidA"
                     /locus_tag="ACGE70_000422"
     CDS             complement(28596..29405)
                     /gene="yidA"
                     /locus_tag="ACGE70_000422"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFF8692089.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(29424..30791)
                     /locus_tag="ACGE70_000423"
     CDS             complement(29424..30791)
                     /locus_tag="ACGE70_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFF8692090.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(30775..31605)
                     /locus_tag="ACGE70_000424"
     CDS             complement(30775..31605)
                     /locus_tag="ACGE70_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFF8692091.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            31768..32205
                     /locus_tag="ACGE70_000425"
     CDS             31768..32205
                     /locus_tag="ACGE70_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFF8692092.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            32281..32901
                     /gene="rpoE"
                     /locus_tag="ACGE70_000426"
     CDS             32281..32901
                     /gene="rpoE"
                     /locus_tag="ACGE70_000426"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFF8692093.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
BASE COUNT        10739 a         5658 c         7084 g         9490 t
ORIGIN      
        1 attgtttgta gaatgaagga aactatttat tgggtagatg aatcaccacg aatagtcaaa
       61 aggagatttc aggatatgcc aaaaatgaaa aaaatcattt tagcagtaat gttattttct
      121 acggttatat cttttacagc cccttttttg cgtacttaag ctgattttaa gttttgaccc
      181 ttagtctgga tctacgaggt gagagtcaag aatgaaaaaa ataattggag cttttgtatt
      241 tgcaatcatt atcattatta tctgttttca aatagatgtt tttggtcaaa aaaagatagt
      301 tggacaaacg gtcagcagta cgaacagtac ggttacaaag acaaaggaat catttgatga
      361 tacagaacaa gagacattaa tcaaaatcgg aaaaaatgac cgtacactat atacagatga
      421 ctcgctaact accccacttg caactataaa cggcggagag ctggcggagt ttctttctga
      481 aacaaaaaat gcttatcaga tcaagacaaa tgatggctat actggatatt tagccctcga
      541 agatggaacc aaagtcacaa agaatataca aacaaaaccg gaagagctat ctgatgcagt
      601 gattgtatta gatcctgggc atggcggaaa tgatactggg gcattaagca atgatgaaca
      661 aacagaagaa aaagatataa cattaagtac agccataaaa gtaaaaaagg cgctagagga
      721 tgctggagca acggtttatt tgacccatac tacagatgaa ttagtacagc ttggagatat
      781 atgcgactac agtgaagaaa aaaaggcaga tgtctttatc agtttacacg cagactctac
      841 ggaatatgcg aatgaagcaa ctggaatcac gacatattat tactatggac aagaggaaac
      901 attagcacag acaattgctg actccttcac tgatctgcct ctggattcaa gaggaatctc
      961 cactgggaac tatcaagttc ttcgagaaaa tcttcaacct tctattttaa tcgagatggg
     1021 ttatatgaat aatgatagtg atttaaagga attagtaaca gacagttatc agcagcagat
     1081 tgctgcaagt ttgacacaag cattgaccac ttattttcag taataaataa gcccagccaa
     1141 acaaaaatag ctcctcatat ttttcgtttg gccgggctta ttatcgcaga tgcaagcttt
     1201 tgaacaccgt ttaaggaaga ggttgtgagc ctgccgttta actccgcgtc acaaggagca
     1261 atttcaaaaa acagcttctc atgtttgatg actattgtga cttgtgaccg aggagttggc
     1321 atgagaacac cgtttattcg gaacaaggga ctgtgacatg acttttgtca cagttccatt
     1381 atttttttag cagcttcaaa gctttttctg tattggcaaa atgaagtttg gcatattttt
     1441 caagcatagg catgccgcct ttctccaata gttttgccgc tacttggtca gatttcgttc
     1501 cagctcctga tggaagggtg attccagctt cagctgatgc tttgtgtaac tcttccaaaa
     1561 agctggcacg actgatgata gaagcagcag caacagctaa atgatattgt tctcctttag
     1621 taacgaagta cagtttttct tgtaattgat ttttttctaa acgcgcatat tttttaaaat
     1681 ttgcttccgg cgtaaactga tcgatcaaaa tcgcttctgg tttttctggc tcgatttttt
     1741 ttagcagcaa atggatcgct tgattgtgca aagcgacttt catccgaacg gcattgtatt
     1801 ttggttggat ctcattgtat ttttctggag ataagacaag cagtttatag gggatggttt
     1861 tttttaagat gtcagcaagc tggatgattt ggctatcttt taactctttt gaatctctaa
     1921 caccaagttt acgtaattct gcaagctgat cacgtttcac ataagcagca catactgtta
     1981 gcggaccaaa atagctgcca tttccgacct catcactacc taaaacggat aattgactga
     2041 tattttttgg caaagaagag gctgtttttg ctggcttggc ttgtttatcc tgtgcctccc
     2101 cccatttatc agcttctatt gctgcattcg taccttgaaa catcactttt ccggatgtat
     2161 aagcggtaat ggtcgtcccg tctttttttg cagcaaagct agtataagga atctgtttat
     2221 ttaacaaata agagcgatag tatgctttca tttgactaag ttggtcgttt gttgctttga
     2281 taatgacgtt gctcataaaa ttctcctcgt ttttatacat aaaactatac cgcaactcac
     2341 gacttttcgt tttctaagag attgcgagac atagcatttt tttaaagttt atcatataat
     2401 agttaagtat gtgaaaaata tttgtggaga acggtgaaag aaccaacttc atggtacact
     2461 aaataaaact ttcatcaaat aggaggaaat gattcgtatg gcgcacgaaa aaacacgcta
     2521 caaagcagtg attgccaacc aaacatatac tattatcgga cgggaaacca agcatcatat
     2581 ggatatcgta acgaagctga tcaatgaaca gttagcagaa ttgaaacaat tatcaccaca
     2641 aatggataat gaacaagcag caatcttgat ggctgtcaat gcgctttcgg atcaattgaa
     2701 gaaacaagaa agaatcttgg agctggaaga agaaacagca gaattaaaga aaaaaatgat
     2761 caaatttaca gagcttgaaa atcgtgtgaa acgcattgaa gcaatcgaga acgaagctcg
     2821 ggaagtattg aaagaaaatg gtcaagcaga tcataccatt aaaaatcatg tggaagcaca
     2881 acagatttta aacgagaaac gaaaaggaca aattaaacaa aaatcttcta attaagatcc
     2941 ttttatcaaa ataagaaaaa tttaaaaaag ttagaagaga aaggaacgta gcatgctgag
     3001 tttgctgatt ttatttttat tgctaattgc gtttttttct ggagcacgtc gcgggtttgc
     3061 tttgcaaatc atattcgcga ttggatatgt attgtcattt attgctgccc agcattttta
     3121 caaaccatta gcaagtcatt tggaattata cattccatac ccagctgtca ccccaacatc
     3181 aaagttagca ttttttgatc aggtatttgc ttttcaccta gatgaagctt tttatgcagg
     3241 aactgctttt ctgattattt tgctaattgg ctggctgctt acccgatttg tgggggtttt
     3301 tgtccatgga ttgacgtatg tgccgatctt gagacaggta gactggattg caggaggcat
     3361 cctgtcacta atcatggctt acgttactat atttttgatt ttgcagttac ttgcctttgt
     3421 tccattagat gtggtccaaa atcaattcgg cgagcatagt ttcgctcgtt ttatcgttga
     3481 gagaacgcca tttcttacca acaagatgta tgagctttgg taacgaatat tttgaattaa
     3541 ggaaaatagt gctggaaaat atttcgtttt ttgtacagag tagtttattt aagaaggctg
     3601 cgtagcgtat gtccggcaca gctctttttt catagtgaaa gataaggtga taaaatgaat
     3661 aaccgaatcc ttgaaacatt ggaatttgaa aaagtcaaac agatggtcag acaatttgta
     3721 gtgactgcac aaggaaaaga agaactggca gaattggtac cagtgagtga gaaacaaacg
     3781 atcacaaact ggcttcaaga aactgaggat ggattgaaag tgcaacgtct gcgaggcggg
     3841 atccctatac ctaaacttga aaatatccgt ccccatatga aacggatcga gatcggagca
     3901 gacttgaatg gagtggagct tgcacaagtt tccagagtat tgtctacgac aagcgaattg
     3961 aatcgtttca ttgatgatct ttctgacagt gaaatcgaat ttgcacgttt gtatatgtgg
     4021 gcagatcagt tagtcacgat ccctgttttg agccgccgtt taaaggaagc aatcgatgaa
     4081 gacggacgcg tgactgatga cgcttctcct gagttaaaga gcataagaca gaacattcgg
     4141 cgaagtgaac aagctgttcg tgaacagtta gatgggatcg tccgtgggaa aaatgcaaag
     4201 tatttaagtg atgcgatcat caccatgcga aatgaccgct acgttatccc agtcaaacaa
     4261 gaataccgcg gtgtatttgg cggtgtagtc cacgatcaaa gtgcttcagg acagacttta
     4321 tttattgaac caaaacaagt ggtggatttg aataatcgtt tgcgtcaata ccaaatcgct
     4381 gaacgaaatg agattcaacg tattttaagt gaactttctg ctgaacttgt tccccatcgt
     4441 caagaaatca tccacaatgc ttatgtgatt ggtaaaatgg atctgatgaa tgcaaaagca
     4501 cgatttggta aagaagtaaa agccatcgtg ccgggaatca gtgaagacaa tcatgtcgtc
     4561 ttgaaacaag caagacatcc actgatcgat caggaaaaag tagtttctaa tgacatcaca
     4621 atcggaaaag actatcaagc catcgtcatt acaggaccta acacaggtgg gaaaacgatc
     4681 acgttgaaga ctttaggtct gttacaactg atggggcaag caggattacc gattcctgca
     4741 ggtgaagaaa gtcaaatcgg tatttttgag gaagtatttg cggatatagg agacgaacag
     4801 tcgattgagc aaagtctatc gaccttttct tctcatatga cgaacactgt agatatttta
     4861 tcaaaagtca atgaaaaaag tctggtatta tttgacgagc tcggtgcagg aacagatccg
     4921 caagaaggtg ctgctctagc aatcgcaatc ttagatgatc ttggtaaaaa gtctgcttat
     4981 gtgatggcaa caacgcatta tcctgaattg aaagtttatg gatacaatcg ggcaaatacg
     5041 attaatgcca gcatggagtt cgatgtggat acactaagtc caacgtatcg tttgctgatt
     5101 ggcgttcctg gacgaagcaa tgcgtttgaa atctctagcc gattaggtct tgatacagaa
     5161 gttattgatg aagcgaagca gttgatgaat gatgagagcc aagatttaaa cgaaatgatc
     5221 acagatttag agaatcgccg gaaaatggca gagaccgaat atctggaaat gcgtcatttt
     5281 gtttctgagg cacaagaact tcacgatgat ttgaaagaag cttacagcta tttctttgaa
     5341 gaacgtgaaa aagaaatgga aaaagcaaag aaaaaagcaa acgaagtcgt gtcagaggcg
     5401 gaagaaaaag cagaaaaaat cattgctgat attcgtaaaa tgcaacagca gattgggcaa
     5461 ggaaatgtga aagagcatca gttgatcgat gcaaagaccc aattagcaaa tctacatcaa
     5521 gaagaaactt tgaagaaaaa taaagtattg aagaaggcga aagaacaaaa aacgttgaaa
     5581 ccgggagacg aagtgttggt tacgacttac ggccaaagag ggacgctttt acgtaaaaac
     5641 gggaatcagt ggcaagtaga aatcggtata ttaaaaatga atgtgtccga agacgagctg
     5701 acgcctgttg ctcctcaaaa agagccgaca caacgagtta tccatgctgt acgatctgaa
     5761 tctagtagtc acgtgccgaa tcaattggac cttcgaggaa aacgatacga agaagcgcta
     5821 agtgaggtag atcaatattt ggattctgcc attctagctg gttatcctca agtgactatt
     5881 gtccacggaa aaggaacagg cgcacttcgc aagggaatta ctgattatct taaaaaccat
     5941 cggagcgtca aaagctttga gtttgcaccg gcaaaccaag gcggaaatgg tgcgacaatt
     6001 gttaaattta agtaaagctt actaaaggta agcagatggt tcgctatcca agtatagaaa
     6061 tgctatacta atcatagatt ttaaaaatgg aggtctttga tatgtcacaa gtaattacag
     6121 ataagacatt taatgaagaa acagataaag gacttgtatt gattgatttt tgggcaactt
     6181 ggtgtggtcc atgtcgtatg caagcaccga ttttggatca gttggaacaa gaatatgatg
     6241 aagaagaatt cagaattgct aaaatggatg tggatgaaaa tccagaaact cctcaacaat
     6301 tcggtatcat gagcattcca acattaatgt tgaaaaaaga tggtcaagta gtagaaaaag
     6361 ctgttggtgt tcattcaaaa gaacaactac gtcaaatgat cgatcaatat ttataaaaac
     6421 ttacagcaaa aataagctga gggtgggact gaatccagtt ccgtcctcgg tttttttgcg
     6481 gttgagttaa ggttattcaa attaatattt tttgagtata attacgttgt caggctggat
     6541 tgtttttttg acagacatgc tataataact ttaattgaat atgaagggga gtaccagatg
     6601 aacgaacgaa tcaaaaacaa attagcttta cttcctgatc agcccggctg ttacttgatg
     6661 aaagataaaa atggaacgat catttatgtc ggcaaagcca aaatattaaa aaatcgtgtc
     6721 cgctcatatt ttactggcag ccataatacg aaaacggaac ggcttgttag tgaaatcgaa
     6781 gactttgaat acatcgttac agaatcgaat atcgaagcat tgctcttaga gatcaatctg
     6841 ataaagaaaa atgatcctaa atacaacatt atgttaaagg atgataaaac ctatcctttc
     6901 cttaagataa cgaatgaaaa atatccccgt ttggtgatta ctcgtaaagt actaaaagac
     6961 aaagcactgt attttggacc atatcccgat gtcggtgcgg cgaatgagac aaagaagatc
     7021 ctcgatcgat tatttcctct gagaaaatgc aaacctagtc agacgaagga gccttgtctt
     7081 tactatcatt tgggacaatg tctgtgtcct tattatttcg acgtagatcc gaaagtctat
     7141 acgaaaatcg ttgatgaagt gaaaaagttt ttaaacggag gacacgaaaa aatcgagtca
     7201 gagatccatc agaaaatgga aaaagctgct gaaaacatgg agtttgaaaa agctgcagaa
     7261 taccgagacc agatccgtgc cattgagaca atcatgactc gccaaaaaat gacaaatact
     7321 gatttactgg atcgagatgt atttggttat gcgattgaca aaggatggat gtgtgtccaa
     7381 gtgtttttcg taagacaagg aaagctaatt gaacgagatg tttcaatgtt tcctttctat
     7441 aacgaagcag aagaagattt cttgacttac ctcggtcagt tttatcagga aaatgagcat
     7501 ttcattccta aagaagtctt gattcctgat acgatcgaca agcccagtgt agaagctttg
     7561 ttagctacga aagtgctgca accaaaacga ggagaaaaga aaaaattagt tgaattggcg
     7621 aataagaatg cctcagttgc tttgaatgaa cggtttgatc tgattgccag aaaacaggaa
     7681 cggacgatcg gagcagttga acgtttagga aacgcgatga atatcccgac cccaatacgt
     7741 atcgagtctt ttgataactc taatatcatg gggacgaatc ctgtggctgc gatggtcgta
     7801 ttcattgatg gaaaaccggc aagaaaagaa tatcggaaat ataaaataaa aacagtcaac
     7861 ggaccggatg attatgcatc aatgagagaa gtcatctatc gcagatattc acgagtgatt
     7921 cgcgaagggt tgcctttacc tgatttgatc ttgatcgatg gaggaaaagg acaagtagat
     7981 gtggcaaaag atgtcttaga aaatcaatta ggaatcgata ttcctattgc tggcatggca
     8041 aaaaacgata aacataaaac aaatgagttg ctattcggta tagatttggc tgtagtaccg
     8101 cttgagcgaa attcaccaga gtttttctta ctccagcgaa tccaagatga agtccatcga
     8161 ttcgcaatca ctttccatcg gcagctacgc agtaaaaaca gttttgcttc ccgattagat
     8221 gaaattgaag ggttaggacc aaaacgaaaa aaaatgctgc ttaaagagtt caaatcgttg
     8281 aaaaatatca cagcagccac gacagaagaa ttacaagaaa tcggactgcc gaaaaatgtc
     8341 gcacaaaacg tatttgataa attacatcag gaatgaggaa agggcacttt acaaagaaag
     8401 taaagtgtcc tatcatttta tgtacaacaa aaagagcgtt tacgttttgt tggtaaataa
     8461 taaatcgctt acgtctggtg tctaaggata tatgtgattt cttgaagaag gagtgaagaa
     8521 atgttagaag tcaaggatct tgttaaaact tttgggaact acacagctgt cgatcatgtc
     8581 tctttccaaa tccccgatgg aaaaatcatg gggctaattg gccaaaacgg ggctgggaag
     8641 acaacgactt ttcgtttgat cttggatttt ttgacacaag accaaggaga agtgttatgg
     8701 aatggccatc atcttgggga gaaagattat gatgtcatcg gttatcttcc ggaggaacgg
     8761 ggactttatc cgaaagtaac gattcaagac caactgcttt attttgcaga acttagaggg
     8821 aaatcaagaa aagagattga accaaaaatc gatttttgga tggaaaaatt tcaggtaaaa
     8881 gggaaaaaga cggataaagt aaaatctttg tctaaaggaa atcagcaaaa agtccagctg
     8941 atcgccacgt taatccatga acctaaattg atcattttgg atgagccatt cagcgggtta
     9001 gatccggtaa atgctgaatt gctaaaagat ggaatattgg aattgaaaaa acatggttca
     9061 tgtgtcattt tttctagcca caatatggat aatgtagaaa aaatctgcga tcatttgatc
     9121 atgttgcgaa acggaaaaat ggttttgaat ggaaaagtac atgagattcg tgaatcattt
     9181 ggccgtacga agttattctt ggaatctggt ttaagccaac aggaagtaga agaaatcgaa
     9241 ggcgtgaaaa aagtgatcct tcatgaagac aatacgttag agattacctt agaagatcct
     9301 gaagctggga aagaaatctt tactcgtgcg actcagttcg gctacattcc tatgtttaac
     9361 cagcaaccgc caactttgga agagattttc aaattgaaag caggtgaaat cgatgcgtaa
     9421 gttttggatt attaccaaag atgtctattt aaaaaatgtt aagtcgattt ctttcttgat
     9481 catgatatta gtccctttta tcatgctggg tatcatttat gtagcaggaa actttgctca
     9541 acaaaatagt gatgtcaata aaatcggcgt attggctgat aatgagcaaa tcagtcagca
     9601 aatgagccaa gtcaaaacag atgattttga gttcaaagca gttgattctg aagaaaaagc
     9661 acaaaaagag ctttcggaag aaaaaatcga tgcgtattta gtagtctcaa ctgagaatga
     9721 taagataaat gggatgcttt atagcgaaaa ttcattagga caaagtacac aactactgat
     9781 acaacaacaa ctgactggct tgcaatcgat gcttcgagca agtcaattag gcatttcacc
     9841 agaagaagta gctagcttga gccaacaagc aagtttgact cgacaaaaag tcaattttga
     9901 ttcaaaagga aaaatgacag ttggagaaga taactcgaca gtccaatatg tcgtaagtta
     9961 tgtagcaacg attgtcttgt tcattattat tttgacgtat gcgcagatca ttgcacaaga
    10021 gatcgcttct gaaaaaggca cgcggatcat ggaagtgatc ctgtccagta cgactgcaca
    10081 aaagcatttt tacggaaaac taacaggtgt tttacttgtt gcattaacgc aaatggcatt
    10141 atacggtatt atttttggta taggatacag tcaattcaaa gatatggaaa tggtcaaaaa
    10201 tatattagac ggtatctcat tggataactt gattggacca ttcttgtggt attctcttct
    10261 attcatgatc tttggcattt tgattttttc tgttttagca gctttatgtg gctctttggt
    10321 caacaaagca gaagatacag caaaagcaat tttgccagtg acttatctat ctctaggcgg
    10381 atatatgctt ggattgatac taggagcttc agatccaaac aatatcgtta ttcggatcac
    10441 ttcgtatatt ccattcttat cttcatatat tatgccggtt cgcttagcaa atgagacagt
    10501 cggtatgggt ggagcattga tttccttgtt ggtacttgta gccgtaacat tcttattgat
    10561 gatcttgtct gcaaacatgt acaaatcgaa tgtactcgtg tatagcgaag gcggattatg
    10621 gagttcattg aaacaatcga tttcaattat gagaaatgaa cgaaaaaaag cataaaatat
    10681 agaaaaatat agaaggaagc tggacattta ttgttcccag cttctttttt gtatagacct
    10741 atttctattt ttttattata ttggatatat atattcattg aaaatagagc ataacaactt
    10801 tataatgttg agtataccaa tttttggagg cgttttaatg gaccgctatt tagatttact
    10861 ttcagagaag ttccctacaa cggaagatgt catcacggaa atcatcaatc tcgaagcaat
    10921 tttgcagctt ccaaaaggaa cagagttatt tttgagcgat atacatggcg aatttcctgc
    10981 ttttgatcat attctgcgta tcggttcagg aaatcttaaa gaaaagatca aagacctttt
    11041 ctctgaacaa atgacggaaa cggagatcag ccaattcact atttttacgg cttatccaga
    11101 atatgtgttg acgactgatt ggtacaaaaa acaagataaa tcccagctga tccatcgttt
    11161 gatcgatctt ttacggttta cgacagtcaa atacactcga tcaaaagttc gaaaaggatt
    11221 gccgaaagaa tatagctata tcattgagga actcttgtat atcgatgatc gtatcaacgg
    11281 gaaaaaagac tatgtaaaaa aaatcattga acaacttgtc ttgatgaagg aagaagaaaa
    11341 atttttaaca aaacttgcgc agacgatcca aaaatctgtc atcgatcatt tgcatatcgt
    11401 gggtgatatt tttgatcgag gaactcaagc agccaaagta atggatcgaa tcatggattt
    11461 ttcctctgta gatatccagt gggggaatca tgatattttg tggattggtg cgtattgcgg
    11521 ctctgaggca tgtttattga cgcttttacg gattgccgca cgctacaact atttatttga
    11581 attagagaaa gaatatggat taaatttacg tccattattt tcttttgccc atcaaaccta
    11641 tcagaagaat ccagtattta cccctaaaaa caggaaagat ctatctgaaa gagagcaaga
    11701 agaattagaa aagatccatc aagcactttc tatcctccaa ttcaaatcag aacatcagtt
    11761 gttgaatcga cgacctgaat ttaaaatgga cgatcgtaaa ttgttggaaa aaatcaacta
    11821 tgaggaatca acgatcgttt tagaaggtca gctctatgag ttgaaaggga cttgttttca
    11881 aacgatccag ccagatgatc ctaaaaaatt cttggaagag gaacaaaaag tgatcgatag
    11941 tttgatgtat tcattccagc actcgttaag aatgcaaaaa cacatgacgt ttttgatcga
    12001 aaaaggggga atgtatctca cgaacaatca gcatgtactg ttccatggct gtatacctgt
    12061 agatgaaaag gggcagctct tagcatttga attagaccaa tcttatcgcg gaaaagaact
    12121 gctgggattt tttgaaaaac agataactga aagcgcaaaa gatttgactg caaatgaaga
    12181 tttggcgacc gatttgattt ggtatgcgtg gagcgggcca ttttcgcctt tattcggcaa
    12241 gagtaaaatg gcaacttttg agcgctattt tctcacagag cctcatacgc acgtcgaaaa
    12301 aagcaatccc tattatcact tgcgagatga agagtggttc agtgaaaaaa ttttggcaga
    12361 attcgatgca aaagaagaag gttcagcaat catcaatgga cacacgccag taaaagtaaa
    12421 aaaaggagag tcaccgatca aagcaaacgg aaaagcattt gttattgacg gaggtctatc
    12481 caaagcttat caaaaaacaa ccgggattgc tggctactca ttactatgca attcttacgg
    12541 tttccaaatc gtgacacatt atccgttctt gcctattgaa caacaatttg caaaaaaatc
    12601 tgatcaaacg aatgtaaaaa aagtgatcga gcagcagctc ccacgcaaac ttaatcggaa
    12661 tacgacaaaa ggaatggaat tacagcaaca gattactcaa ctccaaaggc tgttggatta
    12721 tcgaaaggac gattgagaaa aatgctagcc aacattgctc aatagactaa aaagcgactg
    12781 caacaaaagg cgtgtcgcag tctccttcgg aattagctca aaaaacggta aaatattaga
    12841 agctattttt ctgtttattt ctctaatact caggtcaggg cgctgatttc acgacctctt
    12901 ccttaaacgg tgttctcctg ccaactcctc ggtcacaagt cacaatatcc aacaaacatg
    12961 agaagctgtt ttttgaaatt gctccttgtg actcagagtt gaacggcagg ctcacgacct
    13021 aatcagaaaa acgattaaaa ttcattcaca aagaaatcga ataaacgctg ttcagatgaa
    13081 tctacacgat gagaaagttc cggatgccat tggacaccaa ggattttgtg catattatct
    13141 ttactttgga ctgcttcaat gagtccatcg ttggaaaagg cgatggcttt taacaaagga
    13201 gaaagatctt tgactgcctg atgatgatag gaattcacaa agtatgaatc agggagaagg
    13261 tcacttaata aactgtccga aacgattttg acttcgtgag ttgcaaactg cggggcagtg
    13321 ggctgttgtt cgtgttttac ggaccatttt ggatataatg aaagatcttg ataaagtgtt
    13381 ccctccaaca cgacattcaa tagttgaagt cctcggcaga ctgcaaaaat cggttttctt
    13441 tgtttcaatg cttccaagat caatgcctgt tcgaattggt ctcgttgaat atttgtttcc
    13501 gtcagttttg gatgagggtc ttccatgtaa agttgcggac taacatcctg accgcctgca
    13561 agaatgagtt tgtcgatttt ctcaattagt tgaggcgctg aatctggaga catgactggc
    13621 agaataagag gtaatccttg agcatctaaa actgcatcca caaatccctg cggtgtatag
    13681 cttacttgat ttccttggaa agtatctgtc gctcgaatca gttgattccc ggcaatgccg
    13741 atgattggtt tcatttttat tccccctaaa gtagactaat tgtaacaagg atttaaaaaa
    13801 atttaaacta tgtaagactt gcccctatac ttatctgttc ttgctgtgtt actatctgta
    13861 aagagaattt tcgtgtgaaa ttcatgacga tagttgaaaa tattgcttaa tttgttataa
    13921 taaaaagtaa gcagaaataa aaaggttggg cgtaagcttc aaggatctag ttcttcaagg
    13981 ggtgagaagg acattttatt gaaggggcct gcctatacta agttatcttt agaggtacat
    14041 actttcaatg aaaaaagaaa gcatgtacct cttttttaaa tcgaaaggaa gtgaagcaag
    14101 tgttagaagc tttggaacaa atccgaaaag cagaagaaga aaatgagaag cgtgctcgaa
    14161 ccctacggca gcagttgaag acctatgcag aagaaaaaga gcaagaattg gagataatcc
    14221 aaacgaacag tcggcaacag ctgaaacagt tggtagaaga aaaagaacaa ttcgaaaagc
    14281 aagaattaga aaaagaaaaa atgaaattga aagcagaagc agacaaggtg caagcaactt
    14341 tgaaagacca atatcaaaag catcagagtc aagcaatcgg tgcaatcatt gaaagggtga
    14401 aagaaactta tggccgtcac taagatggaa aaagtgaccc tcatttcaga taaaagaaat
    14461 caggagagga ttttacaagc agtacaagga atccaaaatg ttgaaatccg ggatctgtat
    14521 caaaatagaa caaacaacca gtgggtagaa aaatattttc cagataccac gattattgat
    14581 aaagaagcaa aattaagcgc tttaggcaat cggctgaaag acatccaaga agctatccaa
    14641 ttcatacaac accacggcga tagccgtcaa aagaaactcc atttgaaacg tcaagaactt
    14701 tccttacaag acttggaagc aaattattcc gaagaaaatt tcatgaataa attagaagaa
    14761 gtccttgatt taaaagaaaa gtgggaacag ctaactgaaa aacgtgatcg tttagaagaa
    14821 gaggaagaat ggcttcttca ttggcaacag ctggatattg ctccaagtat gtatgcaagt
    14881 aagacgacca tttttgagat ggggactgtg agtgcagcaa attttgaaag tttcaaggaa
    14941 gaactaagta agcttgatga cgtttatatg gaagaaatag attatacgcc aaaacaagtt
    15001 tatctttcgt atgttgcttt aaaaaaatca gttgctgcta tagaagaaat tgctgggaaa
    15061 tacgggttca ataaagaaac gtatccttat gaacaaaatc ctaaagaaaa attgcaagaa
    15121 accaaacagc aattatccat tgttcacgag cagcaaaaaa agataacgac agcgttaggt
    15181 cagtgcagcg gctatatcca agaatttgaa tgggtagaag aagtcacact tgccgttgca
    15241 gaacgggaaa aagtaaaaga acggtttatc catgccactt atctgatcgt tttgcaaggt
    15301 tgggtagatg cagaagaaaa acagaatttg atgcatgtgt taaacgaaac tgtatcagaa
    15361 gaagatatat acgttagttt cgaaacacca aactcagaag agattcagca ggaagtccct
    15421 actaaattga aaaaccatcc gcttatcgaa ccttttgaat tgctgactga aatgtacagt
    15481 ctgccaaaat atgaagaagt agatccaacg ccttggatga caccatttta tcttgtcttc
    15541 ttcgggatga tggtcgcaga tgttggttac ggattgctga tgctcattgg gacaatacta
    15601 gctcaaaaac tatttgtttt accccgcggg atgaaacgtt tcgtaaaatt ctttgagatt
    15661 ctctcggtac catcgatcat ctggggattg atctacagtt catttttcgg acaggcatta
    15721 ccaaaagaac tatttggtat ccgcttgcct ttcccaatcc tatctacgac ggatgacgtc
    15781 aatacgattt tgattttatc cgtgattttc ggtctgatac agatcttggt tggattattc
    15841 gtggcagcaa aggaaaatat caggaggaaa gattacttga gtgctgtcag tgatggtttt
    15901 gcctggcaag ggatcctgat tgggatcgtt attgcattga ttggtgtcat gttgataaaa
    15961 aatacagcat ttgtttatct tggcgcgggg ctatcgatca ttgctgcttt atgtatctta
    16021 atcgtgccga ttatccaatc gccgtctaaa gtcaagggtg ctgccaaagg agcgtataat
    16081 ctttacggat tgactggtta tatcggtgat ttagtcagtt atacacgttt gatggcacta
    16141 ggtatttcag gcggaagtat tggcgcggca ttcaatatgc tagttgcttt catgccgcca
    16201 gccgctcgat tcagtgtagg gatccttttg atcatcgctt tacacgcact gaatatgttt
    16261 ttgacactgt taagtgccta tgtccatggt gcacgattgc agtatgtcga attttttgga
    16321 aaattttata caggaggcgg acgtgccttt gacccgctga aaacggcgga aaaatacgtc
    16381 aacatcaatc ataagaaaaa aaacaataaa taattggagg atttttatac aatgatggat
    16441 tacttaatta gtcaaaatgg tggaatggta tttgcagtat tagcgatggc aacagcgacg
    16501 attttttcag ggattggttc agctaaaggt gttggaatga caggtgaggc agcagcagcg
    16561 ttaacaacaa gtcaacccga aaaattcgga caggctttga ttctgcaatt gcttccaggt
    16621 acgcaagggt tgtatggatt cgttatcgcc tttttgattt ttatcaactt aggaaatgat
    16681 atgtcagttg tacaaggatt gaactttcta ggcgcttctc tgccgattgc ttttacaggt
    16741 ttgttttcag gtatcgccca agggaaagtt gcagcagcag gtatccaaat tttagcgaaa
    16801 aaacctgaac atgctacaaa aggaatcatt tttgctgcga tggttgaaac atatgcgatt
    16861 ttaggtttcg ttatttcctt cctattagta ttgaatgcct aagcataaag gaggaaaagc
    16921 agatgaacgc cattgaaaaa atcatctctc agatgaatga agcagcagag caagaaagag
    16981 cggcgttaga gcaagaagag cgaatgaaga tcgatcaaaa ctttgaacaa aaacgtacgc
    17041 aaatcgaaac agaacatcaa aaacaaaaag aaaaacaaat cgaattactt gaaaaaaaat
    17101 accgtcagct taggaatcgc caacaagtag aagtacgaca agaaaatttg aatgcgaagc
    17161 aggaattttt acgacgattg tttgcagatg caatagctga aatggagaat tgggatgaat
    17221 ctgaacaaat ccagtttatc aaaaattcat tgtattcatt acctttgaca ggcaaagtaa
    17281 cattcattgc tggagaaaaa tcagcagctt atctttctca gacattgctg gatgaatgga
    17341 acaatgaact tccctttatg atggttttaa gtgatgaaac tgttgctgat caagcgggtt
    17401 ttctgataaa cgatcaagga gttcagtata attttctttt cagcagcctt gtacaagata
    17461 tccaaggaac gatgagtttt gagattgcca atcaattatt cgaataagga gggatcagtg
    17521 tatggaatac cacgaactaa atcccttgat tcgcggaaga gagcttgaac ttttgtccaa
    17581 tgagacattt gaacggatga ttcaaacaaa ttctgtcgaa gcacttggtg aaatattgaa
    17641 atccacgatt tacagtccat atatttatga cggatatgaa tatgacttcg aacagaatct
    17701 tttaaaggag cagagccgtc tatttcgctg gttaaaggaa tcagcaccgg agcctgaaat
    17761 cgtttggatc tatacgatgc gctatacttt ccataatctg aaggttttga caaaagccga
    17821 gatgacaaat caaaatctgg accatttgta tatcgacgat ggattttatt cattagaaac
    17881 cttgaaggat gccattcata cgcaagtttc aaatgaactc cctgaaagca ttatggacag
    17941 tatacgagaa gtacgtgatt attttgaaga gtctaccatt cttcagggaa ttgacgtgat
    18001 ttatgatcga cagtttttga cagagcagcg acggcttgcc gataagctag gatatcaaga
    18061 attgctgaca gaagtcattg cttttattga cttgaccaat atcacaacga tggctcgagg
    18121 aatattgcag aaaagaacat atgggttcat gacgacggta ctttccagct cgggaagtat
    18181 cgaaaaagaa gtatttttga actttgtaga taaagatatg gaagattaca cgaattttct
    18241 attagagaca gattatgctg attttctgcg ccctgtcatc cgtgagggaa atattgatct
    18301 cgtaaagctt gaacagttga aagatgatta tctaagttca ttgtatcaag aagcacaaac
    18361 ccaggctttc ggaccactgc cactactagc ctttttgaac gcaaaagagg tagaaagcaa
    18421 aaaccttcgt ttactggtta tcggcaaaag aagccatttc gaatcagaaa caatcagaaa
    18481 gcgggtgaga caagtctatg acgcataaga tcggtgtagt tggcgacaaa gattctgttt
    18541 tgccattcaa attgtttggc tttgatgttc gttatggtac aaccaagcaa accgttcgtc
    18601 aggcaattga agaaatggca aagaatgagt atggcgtgat ttatgtcaca gagcaatgtg
    18661 cagcattgag cccagaaacg atcgaacgtt acaaggaaca aatgatacct gctatcgtat
    18721 tgattcccaa tcatcaagga tcattaggga tagggctgga agaaattcaa aaaaacgtag
    18781 aaaaagcagt aggtcaaaat atcttatagt atgaaaagga gatagaaatt ttgcaaattg
    18841 gaaaaatcat aaaagtctcc ggacctcttg taatggctga gaacatgtct gatgccagta
    18901 tccaagacat gtgtctagtg ggagatctag gagttatcgg agaaattatc gagatgcgtg
    18961 gcgatgtggc gtctattcaa gtatatgaag agacttctgg aattggtcca ggcgaaccgg
    19021 ttcgctctac tggtgaagct ttatctgttg aattaggacc agggatcatt tcacaaatgt
    19081 ttgacggaat ccaacggcca ttagatacct ttatggaaat cacacaaagt aatttcttag
    19141 ggcgcggagt tcaattgccc gcattggacc atgagaaaaa atggtggttc gaaccaacag
    19201 tagaagccgg agaaacggtg agtgctggag atgttatcgg gatcgtagaa gaaacaaaag
    19261 ttataaaaca taaaatcatg gtgccaaatg ggataaaagg aacgatcaaa acaatcgaaa
    19321 aaggttcatt cactattgat gaaacagtat gtgtcattga aaccgaaaat ggtgacaaaa
    19381 atttgacgat gcttcaaaaa tggccagtaa gacgtagtcg gccaatcaaa gaaaaattaa
    19441 acccggatgc acccatgatc actggacagc gagtgattga tacattcttc ccagtgacaa
    19501 aaggaggagc agcagctgta cctggtcctt ttggtgcagg gaaaacagtc gttcagcatc
    19561 aaattgcaaa atgggccgat gtggacatgg ttgtctatgt gggatgtggc gagcgtggaa
    19621 atgagatgac ggatgtttta aatgaattcc ctgaattgat tgaccctaat acaggagaat
    19681 cgttgatgga acgtacggta ttgattgcta atacatcaaa catgccagta gcagctcgag
    19741 aagcttctat ctatacggga atcacaattg cggaatattt ccgtgacatg ggttatgacg
    19801 ttgctatcat ggcagactcc acttcacgat gggcagaagc acttcgtgag atgagcggac
    19861 gtttggaaga aatgcctggt gatgaaggtt atccagctta tcttggttca cggcttgcgg
    19921 aatactatga acgatctgga agagtcattg cattaggatc agaaggtcgc gaaggaagta
    19981 taaccgcaat cagtgctgta tccccgtctg gaggggatat ttcggaacca gtcacacaaa
    20041 atacattgcg ggtcgtaaaa gtattctggg gtcttgattc cagtttagcc caaaaaagac
    20101 attttccttc gatcgattgg atacaaagct attctcttta ttcaacagaa gtagggaagt
    20161 atatggatca tatccttcaa gctgactggg catccatggt caccgagggc atgcgtattt
    20221 tgcaggaaga acagcagctg aatgaaatcg tgcgtttggt agggattgat tccctttcag
    20281 ataatgaccg tctgacatta gaagtggcaa aatccattcg tgaagattat ttgcagcaaa
    20341 atgcttttga tgatgtcgat acattcactt caagagagaa acaattcaaa atgttgaaga
    20401 tgatcttgac ctttggtgaa gaaggacgcc gtgcgttgtc gttaggtgct tatttaagtg
    20461 agatcatgga cggtaccgta tcgattcgtg aaaggatcgg acgaagcaaa tatatcccgg
    20521 aaacagaatt agaaaaacta gatcaaatta ttggtgaaat caagcaaacg atgcaaacga
    20581 tcgtttcaga aggaggcatg agcaatgatt aaagaatatc gtacgatcgg tgaagtcgtc
    20641 ggtccattga tggcggttga aaaagttgct ggtgtcaaat atgaagaatt aatcgaagtc
    20701 cgtatgcaaa atggcgagat tcgtcgtgga caagtattgg aagtccaaga agataaagca
    20761 atggtccaaa tattcgaagg aacaagtggt atcaatttac gtgactcttc tgtacgattt
    20821 ttaggccatc ctttagaact aggtgtatcg gaagacatga tcggtcgagt attcgatggt
    20881 ttgggacgac cgaaagataa cggaccagag atcctgcctg aaaagcattt ggatattaac
    20941 ggagaagtaa tcaatccagt agctcgggat tatccagatg agtttatcca aacaggtatc
    21001 tctgcaatcg atcatttgaa tacattagtt cgtgggcaaa aattaccagt attttctggc
    21061 tctggtcttc ctcataaaga attagctgct cagatcgcgc gacaagcaac ggtactcgat
    21121 tcatcagatg atttcgccgt agtctttgct gctattggaa tcacttttga agaagctgaa
    21181 tttttcatgg aagattttcg tcaaacgggc gcaatcgacc gttctgtcat gtttatgaat
    21241 ttagcaaacg accctgcaat cgaacgtatc gctactcctc gtatggcttt gactgctgca
    21301 gaatatcttg cttatgaaaa gggaatgcat gttttggtta tcatgacaga catgaccaac
    21361 tatgcagagg cattgcgaga gatttctgca gcacggcgag aagttccagg aagaagagga
    21421 tatcctgggt atctttacac gaacttagct actttattcg aacgagcagg aagaatccgc
    21481 ggactaaaag gatcggttac tcaaattccg attctaacaa tgccagaaga cgataaaact
    21541 catccaatcc ctgacttgac tggttatatt acagaaggac agatcatctt gactagagag
    21601 ctttacaaga gtgggattca gccaccaatt gatgtactcc cgtctctttc gcggttaaaa
    21661 gataagggaa ctggacccgg aaagacaaga gaagaccatg cagctacgat gaatcagctt
    21721 tttgcagcct atgcacaagg aaaacaggca aaagaattag ccgtagtttt aggtgaatct
    21781 gctttatctg atgtagacaa gatctatgcg aagtttgctg agcggtttga aaaagaatat
    21841 gtcaatcaag gattctacac gaaccgttcg attactgaaa cacttgatct agggtgggaa
    21901 ctgctatcga tgttgccaag aaccgaatta aaacgaatca aagacgacat gctggacaaa
    21961 tatctgccag aagggaagtg agttctgatg gcacgattga atgtgaatcc gacccgcatg
    22021 gaattgactc gattgaaaaa acagttgaca actgccacaa gagggcataa attattaaaa
    22081 gataagcaag atgaactgat gcgtcaattc atcctgttga ttcgaaagaa caaccagctg
    22141 cgtcaagaaa tggaagagtc tatgacagaa gcaatgtctg attttgtatt ggcaaatgct
    22201 tcgatcacgg aacctttcat cgaagaatta tttgttttgc cggcggaaaa cgttgatttg
    22261 tctatcgtag acaaaaatat catgagtgta aaagtacctg tgatgaattt tgaatatgat
    22321 gaacagctga cagacacacc tttggaatac gggtatttga attccagtgc cgaactggat
    22381 cggtcaatcg atcggtttac taccctgctt ccaaaacttt tggaactgac agaaatcgaa
    22441 aaaacttgtc aactgatggc gagtgaaatc gaaaagacac gtcggcgagt caatgcatta
    22501 gaatatatga cgatccctca attagaagaa acgatctatt atattcgtat gaagctggaa
    22561 gaaaacgagc gcgcagaagt aacaaggttg atcaaagtaa aaaatatggg taccactgac
    22621 taagtggtat ctttttttaa aagaggaaaa cgatgattaa acaaagcaaa caaataaaga
    22681 aacgtttgcc ggctgttcag cttctcgcat taggattttt tctcctgatt ttagttggcg
    22741 gcggcctact taccctacca ttttttagta atagtgggca tgggacaaat tttatcgatg
    22801 cgcttttcac tgcaacttct gctgtctgtg taacgggatt gacaacattg aatacagcag
    22861 agcattggaa cagtgctggt caatttttga tcatgctttt gattgagatc ggcggtcttg
    22921 gttttatgat ggttccgatt ctatgctttg cattgataaa gaagaaagtc agtctcagca
    22981 cacgtattat attaaaagaa gcgctgaatc tagaagaagt atcaggtgtg atgaagctca
    23041 tgctttacat cctaaaattg gcctttgtga tccaattgat tggtacggtt gctttatctt
    23101 ttgtatttgt gcctgaattt ggctggttga aaggtatctg gtacagtttg ttccatgcaa
    23161 tctctagttt ttgtaatgca gggtttgatt tgttaggaga cagtttagcc aatcaccagc
    23221 aaaatgttta tttgatcatg gtggtatctg ctttgatcat ttctggtggg ttaggcttca
    23281 tcgtatggcg tgatttactg gaatatcatc agaaaaagaa gatgacgatt cattcaaaaa
    23341 tcgcattaac cgttacaggt acccttttat taggcggatt cgttgtgttc tttttcacag
    23401 agcgcaatgg ggcaacgcta gtggaaggga atatgtttca acgattggca aacacctttt
    23461 ttatgagtgt gacacctaga acggctggat attattcagt tgattacctc catatgagcc
    23521 atgcaggtct gattttaacg atgtttttga tgtacatcgg cggaacatct gggtcgacgg
    23581 ccggcggatt gaaaacaact acgttaggtg tgttgctgat ccagatgcgt tcgatgttga
    23641 aaggacgtac gagagcggaa gcatttggcc gtaccatccg acaaggcgca gtgttgcggg
    23701 cacttacttt attttttgtt acgttgacgt tgtgtgtagt cgccatcatg attttatcgg
    23761 tgactgaaac gatacctaaa acttctggca tcgaatatat tgcttttgag gtcttctctg
    23821 cgtttggtac agtaggtctt acgatgggat tgacgccaga cttgactgca atagggaaat
    23881 tgatcattat agcgcttatg tacattggac gagttggtgt attgactgtg gtcttctctc
    23941 tgatgacaaa agcacaaaga acacaatcaa aatacaaata tccagaagaa agtgtcatga
    24001 ttggataatt tcatcttctg acagaaacct agtgatttag taaaataaga aaataaaata
    24061 atttttcttg cttcttggat actttaagtg caaaatgttc gtttattttt tgaggcacta
    24121 cccgttgttg aacctcgggt agtgcctctt ttttattgaa aggaatcaat aagatttgat
    24181 tcctttattt atcaataggt tataattcat atttttttat tttttctttt acgatactta
    24241 gtccagaagc taaaatttca aggttattag aggataggga aattcgaacg aatgcatctg
    24301 gttgcgcacg tgtagtaaag cgtctggaat gataaagtcg gataccgtta gcaagaaagt
    24361 cattctcaat tatcttaggg tcaatctcag gatcaactgg aagaattcga tagtaaggga
    24421 cacgattcgt tggtttttct actttgaaaa gagaatcaaa aagctgattg gctctttttg
    24481 taagacttaa tttagtttct aagatcctat ttgcttcatt tgaatggagg atttgagtga
    24541 tgacttccac atctaatcca gaagttttca catttatatt aaaaattgct tgttctatct
    24601 tgctttggac atgtactgga actacaagat aagcaactcg caatccagaa caaataaatt
    24661 ttgtcattcc agctaagtag attgtttggt taggtaataa attttggaaa ggaggaatga
    24721 tcgtatgttg atagtaagtt gttaaaaatg aatggatatc atcttcaata acccatagac
    24781 cttctttttc aatcacttcg gctaattgct ctcgtctttt gtgagagatt tgaaacccta
    24841 taggattatt acaagaaggc ataagaaaga caccatgaat cttctttttt ctacattcct
    24901 gaatcaatat atcaggtcgc attccttgct cgtcaaaatc aattggaacg atctctaaat
    24961 gatataactg agccaattca atgaaattag cgtatgtata tcggtctgtt gctattctat
    25021 cacctggaga gaaaagtgca gaaagtgtga tagccaatcc attttgaaca ccggaaacaa
    25081 tagatagaga ttctaaggac ggaatgactc cttgagtttt taaccaattt gctgcagtag
    25141 tcaattgaaa atttgtgcct ttgggatctt ggtaattaag caaaccaaca agatttgggt
    25201 tattactagt tgattgaata aacgggacaa cgaatttatt acattcttca aaagaactaa
    25261 ccagaccaaa atcaatgata tgttcggtta cttcattggt agagatggtg atggactcaa
    25321 agacattggg agaaataaat gtgccacttc caatattcgt atacagcaaa cctttctcaa
    25381 ttccatattt ataagccttg ccaacggtag taaaatttaa atctaaataa tcagctagct
    25441 ctctttggga gggtaactga gtgtttttct gtaatttatt ctttaaaata tcttgttcta
    25501 attgttgaat caacgatcga taataaggac gcttcaattt ttgtttgtct ggtcgccaag
    25561 ttaacgggta attgtcataa gaattaatag gcattttctc cactccttaa aattgtatac
    25621 catacaattt taccattgat tgtattgatt ttagaagtta atcttttgtg aggagggaat
    25681 tgtctatgaa aattattaat tttgaggaag tagtagcttc ttttagtttt gaagaagcga
    25741 ttcaagtaat gaaaaaatgt ttttttgatt atgaaagtgg taaaatcagc caaaatcctc
    25801 gattggtgga aattttacct gacggaaaag gcaaaaatat gtttgcgatg atgcctgctt
    25861 atttggggga gaacagatat tttggcacaa aagtcattac cgcttttccg gataatcatc
    25921 agtatcattt accttctcat ttaggagaga ttttcttgtt tgatgctcaa aacggactac
    25981 ccaaagcatt gataaacgca aatgcagtca cctggattcg aacagctgct gtatctgcgc
    26041 tggcaacaga ttacttagca aatccgcatg cagaaacact tgctttaatt ggagctggtc
    26101 aacaagcatt gtcgcatttg actgcgattt ctaccattcg tcccatcact acagtgtttg
    26161 tttacgatat tttagaagaa agacgaaata cttttatcgc taaagctaaa aaacagtatc
    26221 ctaaaataac gtttataaat gcaatatcag tagaagatgc agtacgtcca gcggaaatca
    26281 tttgtacgtt gacctctagt aaagaggcat ttcttcaaaa agagtgggta agggaagacg
    26341 cacatatcaa tgctgtgggc acatttactc ctgatacaag agagataagt acatcgttga
    26401 tggaaacttc tcaaatctat gtagatgatt actcagctgc cttaacagaa agcggggacc
    26461 tgcttatagc catggcagaa ggagatttag aggaatcttg tattgttggt acattaggcg
    26521 aattagtttc tgagaaaaaa acattttata aaggagaatc gaggccgacg attttcgacg
    26581 cagttggtct agctattgaa gatctttgtt gtgcagaata tatttataac aaattagaga
    26641 aaggggcttt aaaatgaact tagattatta cgtagttgat gcatttgcag atgaggtgtt
    26701 caaaggaaat ccagcagcgg tttatgtgct agaagaatgg ctgccagaag gtacgatgca
    26761 aaaaattgcc attgaaaaca atttatctga aacagcattt acagtgaaga aaaaccaaga
    26821 atttgagctt agatggttta cgccagatcg agaaatcgat ctttgtggtc acgcgacatt
    26881 agcaacagca tttgttttat ttaactatta caaaatacct gatgaaacga ttaaattttc
    26941 tactcaaagc ggtaatctat ttgtgacaag aattaaagat tattattaca tggattttcc
    27001 tagtattatg ccgaaaaaag tgccgatatt gtctgaatat gaagaagcga ttggggctaa
    27061 gattaaagaa gcctacttag cacgtgattt atttttcgta ttagaagatg aaaaaacggt
    27121 ggctaaattg cagccagatt ttacagctat caaaaatttt gaattaggaa taggagtcat
    27181 cgtgacagca gaaagtgtgc aaaaagactt tgtttctaga accttctttc caaaattaac
    27241 aatcaatgaa gatccagtat gtggatcagc acattctaat cttattcctt attgggcaga
    27301 aagaatgaat aaaacaaaat tgtcagctta tcaaatgtca tcaaggggcg gggatctaga
    27361 atgtgaatta aaagaagatc gtgtgataat aggaggaaaa gcagttttgt tttctaaagg
    27421 tacctcgttt atttgatttc tttcaaatga attaaaaata aattggtgga gagtggcaac
    27481 cttatagcta caatggttgt taaaaaaaga cgcaaacttt ttcaatttaa tgaaaagttt
    27541 gcgtctcatt ttaattattg gtgtttgttt tttattcaat caagagtaaa atgccccata
    27601 ctaactatcc tcggatgact tcgattttat aaccatcagg gtcaatgaca aagtagtaag
    27661 aaggcgcagt gccaggtaat ccttttaagt cagtaatctc aagaccagca gctttatgtt
    27721 gctcgtgtaa tttttctaga tcatcagctg ctatagcgat atgaccatag ccgtctccta
    27781 gatcatacgc accatgatca tagttgtaag tcaactctaa ttcatagtca tccccaggaa
    27841 gtgtcaaata aacaagtgta aatttgtttt ctggaaaatc acggcggcgg ctttcttcaa
    27901 aaccaaatgc cttttgataa aattcgattg atgcttcaag atctttcact cgaacacatg
    27961 tgtgagccat tttcatagat tcatctctcc ttttcctttt actcaatcat aacaaaaaga
    28021 agtaaggaaa tagttgattt gtgcttgttc atgagaaatt tctcatgtaa aatggataga
    28081 aaataaaact gttaaggtgg aaactattca tggataaacc aatttttggt gaaaaaaagc
    28141 aatttgctga ttatcaaacc cgttatgcag catatattgt catagaaaga gatcagcaga
    28201 ttgcagtaat cgaggctcca aatggtgctt tttttcttcc tggcggggaa attgaaggag
    28261 aagagacgaa agaagaagcg attgtccgtg agctgataga agaaatggga attgtggcag
    28321 aaatttcttc ttatctggga gaagctgatg agtattttta ctcgaattat cgtgcaactt
    28381 attattataa tccaggatat ttctttgttg ctgattcttg gaagcgtata ggagaaccaa
    28441 ctgaaaagac caataaaatt tggtgggtga gccctcaaga agcgcttgtg aaattgaaac
    28501 gtggtagcca ccgatgggca gtcgagagat ggatcgaaaa aaataaaaac tgaaagaaag
    28561 cgagtagatc agctttctct cagttttttt atttattaca gtacatattt tttgattgct
    28621 tcagctacac cgtgctcgtc gtttgatgaa gtgatgacat ttgctgcttc tttgacaaga
    28681 gaaacggcat ttcccattgc tactcctaga ccagcatatt caatcatgga taagtcgttc
    28741 tcgttatctc cgatagccat gatttcatct tgatcaatac caaggtgctg tgcgagattc
    28801 gcaactgccg cacctttact tgcttcttta tttagtactt cgaagtaata gggcgcactt
    28861 ttgaccgttg tgtatttatc acggaaagtt tgcggtaaac gcgcaattgc tgcatctaaa
    28921 atttctggtt cgtcgatcat catcattttg acgatgttca tgtctggtgt catttcttca
    28981 ggtgttcgat attttagcgg catcttgact aaacttgctt cgtggatcgt ataagcgctg
    29041 atatctcgat taggcgtgta gatcgctttg tcatcgatcg aatggagatg actgccgaca
    29101 cggcgtgcca ttacttcgat ctctaggaaa tcttcatgtg tcagtccgtg acgggcgatg
    29161 attttacctg atttcgtttg ttgtacgagt gccccgttat aagtaataac gtaatcatta
    29221 tcttgaaaaa ggttcaactc gtttaactgt tcctggacac ctggtaaggg acggccagtg
    29281 cataaaacga tcgaaacacc ttttgcaaca gcagcggcaa tcgcttcttt tacttctttt
    29341 gttacttctc ttttctcatt taataaagta ccatcgatgt cgatagcgac tagtttgata
    29401 gacataattt cctccaatat attttaattt atttcaatca atgcgccatt acggatatgg
    29461 ctggcaaatt cttgataagt ttcgtcaaat aaatcataat gatcacgcaa ggacggatca
    29521 accatttctt ttggaaagaa aaatcgctca tctccttgtt cttgtcccgc taatgcagca
    29581 acaagctgac taacctgtga taattctatc aaagttccgt catttcgcaa aatctcgatt
    29641 tgtgtgcgat gtctgccttg ttctgggcga taaaaatcat aaggtagatc gtagctagaa
    29701 ttcacagcag tgtaataaac aggattatat ccgacctttt ctactaacaa agtgagttct
    29761 tggactaatt ttgaatcccg gttgccagaa aaagtagcag atttcagtgg tttacgattc
    29821 aaaaaacgtt tagctaaatc atttaaaata ggatcagcag attcagacca ttgagtaaag
    29881 taagtgccca agaccccatc atcaagtttg aggtattctt ctaaagtaaa gcttccgtca
    29941 agaaatggca gaagtaattg agaatggagt tcaaaactgc tggcatcttc ttgatacaac
    30001 tcgtgtgcgc gatgaagcag atgctccaaa attacttcca taccacggga aacaggatgg
    30061 aagtacactt gcacatacat ttgatatcgg ctaacgatat aatcttccac tgcatgcatg
    30121 ccactcatgg aaaaagcgat cccgccttca taaggacgaa tcacacgtaa tattcgggta
    30181 agatcaaatg ttccatattc tgttccagtg aaataagcat cccgaagcaa atagtccatt
    30241 cgatctgcat cgatctgact agaaatcatt tggacaactt gtgggttggg gtaagttttt
    30301 tggatcacac tagctacctt ttcaggaaaa ccttcttcta ctcggttaag aatctgatac
    30361 acttctgttt ctggagaagt gatgatctct acagtgatgg cttcatgatt cgtatggaag
    30421 atatgttcaa atgtatgaga gtaaggacca tgaccgacat catgcaacag tgccgcacat
    30481 aaagtcacca gccgttcctg atcatcccag cctccgttgc ctattttttc ttttgaaaaa
    30541 tttcgagaaa agatatcgca gattcgtctt gaaatctcat aaacgcctaa tgaatgagta
    30601 aaacggctat gttctgctcc gtgaaaagtg aaagaagaag tgcctaattg tttaatgcgt
    30661 cgtagacgtt ggacttcttt cgaattgatc aaatctaaga ttacttgatg ctggacatgg
    30721 atatagttat ggactggatc acgaaaaact ttttccatag gcagcatttg atatttataa
    30781 ggggttgtca taatgtagct cctttatgtt attacgttgt tccattctct ggatttggtt
    30841 gttccattgc tccagctgga tttctttttt tagccagtcg gtagaatcct gtaaatcact
    30901 tgcagaatac agcttttcaa aagcttttgc aaatgcttct ttgacttctt ttacaaaaag
    30961 gggttggtca agcagttctt caagtgttgc cattgtagca ggatccactg caggatagcc
    31021 atttgtacca aagtcttctt ttaaagcaga ttgataaaat cgcttcatga cttgcccacg
    31081 tttttcctga ttaccgttga cacttaagta catcataaca gaaatcccat ttttgatccg
    31141 acgctgagcg ataccggcaa attttttgcc atggatgctt aaatcataag ttccaggaca
    31201 ataagaatgg gtgatttcga atgcttgtat cggtgccttt ggaaaagcgt atttagtcaa
    31261 taggagcatt tgctcgtaag cgtcatctat ggttattttt tttgtgtcac tttgggggaa
    31321 aatcaaagaa atattcaaaa ttccttcatc tgatacgatc cctaatccac ctgaatttct
    31381 gatcactgga gcgtaacctg catcttccag tgtttggatc ccgtcactta gaaaaggagt
    31441 tcgaacatcc ttcataccta agatgactgt gcagtcatat tgccagaaat gtaaaaagat
    31501 ttgctggtgt tctttagaat attccgttaa tatatctgtt aatgcaaaag gggaaaagtc
    31561 gtttttttga taaatgtgct gatctagtcg aatcgcaggg ttcattcaac cggctccttt
    31621 gtataaattc atattttcta caatattata ctctaaaatc aaagagttgt tttggaaaaa
    31681 aggttttctg taaaaatgga caagttggtt gacaaaacaa aagaaactag gaacaatgta
    31741 gctatgagaa gtaaaggaga atgaataatg gaattatcac aaggaacacc gatttcaatc
    31801 aaattgcgta caaaagtaaa acagcaaggg gaagtgcaag atttttattt tgatttgaaa
    31861 gggcaaatgg tgacaattgg cgatacgctg tacatacgtt ataaagagat tcaggaggag
    31921 acgggcgatg agattcctgt aacgatcaaa ttagttccag atggtcatgt acagctgatc
    31981 cgtgcaggag aaatgcggat gcgtttgaaa tttggctata aagaacggtt agaaacatcc
    32041 tatcgaacac catacggtat gatccagatt gctactttta cgaaagaact gcatgttagt
    32101 ttgaaagacc gtcccacagc agggaaggtt cgaatcgatt atgatctgtt catgggaccg
    32161 gaaaaaatcg gcgaatatta tttgaccttg gattttacag cgtaaatata aaagaaaagt
    32221 ttgatttttt gtgtctgatt ttgtatgata aggagtagac tgtgaaagga cgtgtcctga
    32281 ttggaaatta gtgtatttga aggtgcaaac aaaagcgaat tatcaatgat cgaagtagca
    32341 cacgcgatct tagaacaacg tggcgatgtc atggattttt ctgatttagc taaccagatc
    32401 caaaactatc ttgggaaatc agacagtgaa attcgtgatt cactcgcaca attttatact
    32461 gacttgaata ttgacggcag ctttatctca ttaggggata atcgttgggg cttgcgttca
    32521 tggtatgcta tcgattcgat cgatgaagaa gtaaaccatg gaatggacga agaggatgaa
    32581 gatactccac gtcgccgtaa acgcaagaaa gtcaacgcct ttatcaatga tgatgaagac
    32641 gcgatcgatt acaatgacga tgacccagaa gatacagatc tgacagaaga agatgacgat
    32701 gatctgtttg atgacgatga tgatgaagat gaagaaatcg ctgcttataa ttctgacttg
    32761 caagaaatcg gagcagatga cccgacagat gatgaagaag acttgccagg aaatatcgaa
    32821 gaagatttaa gtatcataga cgaagacgat gatgatgaag attatgaaga ggaagaattt
    32881 tctgactctg aaaaagaata aacattattt tcttacgaga ggttgtgaca gagacgatca
    32941 gcctatcaga ataaggaggt gtgacacgat t
//
LOCUS       DAXWOO010000011        31371 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.011, whole genome shotgun sequence.
ACCESSION   DAXWOO010000011 DAXWOO010000000
VERSION     DAXWOO010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.011"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(338..2230)
                     /locus_tag="ACGE70_000427"
     CDS             complement(338..2230)
                     /locus_tag="ACGE70_000427"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8692094.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2227..3954)
                     /locus_tag="ACGE70_000428"
     CDS             complement(2227..3954)
                     /locus_tag="ACGE70_000428"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8692095.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3970..4620)
                     /locus_tag="ACGE70_000429"
     CDS             complement(3970..4620)
                     /locus_tag="ACGE70_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFF8692096.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4824..5120
                     /locus_tag="ACGE70_000430"
     CDS             4824..5120
                     /locus_tag="ACGE70_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFF8692097.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5312..5932
                     /locus_tag="ACGE70_000431"
     CDS             5312..5932
                     /locus_tag="ACGE70_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFF8692098.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(6024..7190)
                     /locus_tag="ACGE70_000432"
     CDS             complement(6024..7190)
                     /locus_tag="ACGE70_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFF8692099.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7202..7555)
                     /locus_tag="ACGE70_000433"
     CDS             complement(7202..7555)
                     /locus_tag="ACGE70_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFF8692100.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7683..8705
                     /locus_tag="ACGE70_000434"
     CDS             7683..8705
                     /locus_tag="ACGE70_000434"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFF8692101.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8724..8885
                     /locus_tag="ACGE70_000435"
     CDS             8724..8885
                     /locus_tag="ACGE70_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692102.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(9144..9578)
                     /locus_tag="ACGE70_000436"
     CDS             complement(9144..9578)
                     /locus_tag="ACGE70_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692103.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9688..11547)
                     /locus_tag="ACGE70_000437"
     CDS             complement(9688..11547)
                     /locus_tag="ACGE70_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFF8692104.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMVLFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            11548..11688
                     /locus_tag="ACGE70_000438"
     CDS             11548..11688
                     /locus_tag="ACGE70_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFF8692105.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(11665..12824)
                     /locus_tag="ACGE70_000439"
                     /pseudo
     CDS             complement(11665..12824)
                     /locus_tag="ACGE70_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
     gene            complement(13131..14555)
                     /gene="araA"
                     /locus_tag="ACGE70_000440"
     CDS             complement(13131..14555)
                     /gene="araA"
                     /locus_tag="ACGE70_000440"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFF8692106.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14571..15269)
                     /locus_tag="ACGE70_000441"
     CDS             complement(14571..15269)
                     /locus_tag="ACGE70_000441"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFF8692107.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15226..16887)
                     /locus_tag="ACGE70_000442"
     CDS             complement(15226..16887)
                     /locus_tag="ACGE70_000442"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFF8692108.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            17105..18505
                     /locus_tag="ACGE70_000443"
     CDS             17105..18505
                     /locus_tag="ACGE70_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFF8692109.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18757..19836)
                     /locus_tag="ACGE70_000444"
     CDS             complement(18757..19836)
                     /locus_tag="ACGE70_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFF8692110.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19922..20881)
                     /locus_tag="ACGE70_000445"
     CDS             complement(19922..20881)
                     /locus_tag="ACGE70_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFF8692111.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDTLIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20893..22404)
                     /locus_tag="ACGE70_000446"
     CDS             complement(20893..22404)
                     /locus_tag="ACGE70_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFF8692112.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22426..22989)
                     /locus_tag="ACGE70_000447"
     CDS             complement(22426..22989)
                     /locus_tag="ACGE70_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFF8692113.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22995..23858)
                     /locus_tag="ACGE70_000448"
     CDS             complement(22995..23858)
                     /locus_tag="ACGE70_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8692114.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23872..24768)
                     /locus_tag="ACGE70_000449"
     CDS             complement(23872..24768)
                     /locus_tag="ACGE70_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8692115.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24943..26232)
                     /locus_tag="ACGE70_000450"
     CDS             complement(24943..26232)
                     /locus_tag="ACGE70_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFF8692116.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26204..27721)
                     /locus_tag="ACGE70_000451"
     CDS             complement(26204..27721)
                     /locus_tag="ACGE70_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFF8692117.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            27919..28809
                     /locus_tag="ACGE70_000452"
     CDS             27919..28809
                     /locus_tag="ACGE70_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFF8692118.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(28882..29706)
                     /gene="nadE"
                     /locus_tag="ACGE70_000453"
     CDS             complement(28882..29706)
                     /gene="nadE"
                     /locus_tag="ACGE70_000453"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFF8692119.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29718..31190)
                     /locus_tag="ACGE70_000454"
     CDS             complement(29718..31190)
                     /locus_tag="ACGE70_000454"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFF8692120.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
BASE COUNT         9150 a         6596 c         5447 g        10178 t
ORIGIN      
        1 aaactggctt ttgaacaccg tttaccaaga ggttgtgagc ctgccgttta actccgagtc
       61 acaaggagca atttcaaaaa acagcttctc aggtttgttg actattgtga cttgtgaccg
      121 aggagttggc aggagaacac cgtttaagga agaggttgta aaatcagcgg tttgacctga
      181 gtattagaga tacaaaacgg aaaaatagct tctcatattt tgccgttttt tgagctaata
      241 ccgaaggaca gctgattgaa caccgtttat tcggaaaaag ggactgtgac acgacttttg
      301 tcacagcccc taaaaaaaat aataaatttg tactattcta tgaagcttct tcttcagaaa
      361 actgactgtt ataaagatct gcatagaatc cgtctttttc catcaatgta tcgtggtttc
      421 ctgtttctac gatcgaacca tgattcatca caataatgtt gtctgcatcg cgtattgtag
      481 acaagcggtg agcaacaacg aaacttgtcc ggttttccaa taggcggttc atcgcttttt
      541 gaatcagtat ttctgttcgt gtatctacac tagatgttgc ttcatcaagg atcaggacat
      601 ctggatttgc taagaatgcc cgcgcaattg tgattagctg acgctgccct tgcgaaatat
      661 tgcttgcatc ctcgtttaat acagtgttat atccttctgg caatttacga acaaaatcgt
      721 ctacgtgagc agctttagca gcctcaatga tttgttcgtc tgttgcatcg tcgttcccat
      781 atttgatatt gtcatagata cttcctgtaa acagccaagt atcttgcagt accattgaaa
      841 actgtgcgcg caattcatca cgtgaaagat cacgggtatc tacaccatcg taacgaatac
      901 tacctccaga aacatcgtag aaccgttcaa gaaggttgat cagtgtcgtc ttccctgcac
      961 cagtcggacc tacaatagcg accatctcac caggtttgac atccaagtta aaatcagtca
     1021 ttagcaagtt atctggtgtg tagccgaatt gaacattttc aaaacgaact ttgtatggtg
     1081 tatctttttc ttctggaagg ccagattttt cattggacat ctcttcttca tccaacactt
     1141 caaatacccg ttcggctgaa gcaactgttg attggatagt gttcatcaaa cttgcaagtt
     1201 gagtgattgg ttgagagaac tgtgttgtat attgcaagaa cgcttgaaca tcccctaatg
     1261 tcatgttccc atttgcaact tttactccgc ctagtacagc gacgaacaca tagcctagat
     1321 ttttaacgaa attcatcaac ggcatgatga ttgccgaaat aaattgagct ttccaaccag
     1381 cggcatacag attctgattt tctttttcaa atacttcctg gtctttttca gtatgattga
     1441 atgttttaac aatggtatga ccaccatatg tttcttctac ttggttattc aataaaccta
     1501 aacttttttg ctgatctgcg aaaaacactt gtgatttcgg cgctacgatc atcacgatga
     1561 tcaagcttaa agggatcatc aataacgcga ctaacgtcaa ttgccagctg attgaaagca
     1621 tcatcacgat gaccccaatc aatgtgactg cactagttac aaattgagtc aaattttgct
     1681 gcagcgtgct tgcgatgtta tccatatcat tgattgctcg agacatgata tccccatttg
     1741 tatgtgtatc ataataggaa atcggtacac gattcatttt ggcttccaaa tcatgacgta
     1801 attcataaac tgttcgttgg gaaacacgtg tcataataaa ttgctgtaaa aagttgaata
     1861 cagcagaaat caaatacatc acgattactg tcgcaatgat ctgcgcgatt ttatcaaagt
     1921 ctatcggaaa ttttgttact tgttggcctt gttgcatctg ttgagcgcca gccattacac
     1981 ctttgaatat ttctgtggtt gctttcccca agacctttgg tgtttggatt tggaagatca
     2041 cggctgctat tgctagtaca aatacagcga tgatcgcaac catacgttta gacatatagc
     2101 caaacaatcg gcgtaccgtt ccccagaaat ttttaggctt ttcacctttt acaccgagat
     2161 ttctgccagg tccaccatgt cctggaccgc ctgctggtcc atgagaggga cgtgtgtttg
     2221 ttttactcat gctaagtcct cctctcttag ttgtgaatga acgatttctt gataagtttc
     2281 gtttgttgct aatagttctt catgtgttcc tttgccaacc agttttcctt catctagcac
     2341 aaggatcata tctgcatcca ttactgtact tactcgttga gcaactaaaa cggtgatact
     2401 ttctttcata ttcgttttca atgcttggcg aagattggca tccgttttga aatccaacgc
     2461 tgagaaggaa tcatcaaaca catagatatc tgctgttttg actaatgccc gtgcaattgc
     2521 cagacgctgt ctttgtccac cagagaagtt tcctcctcct tgttccacat ggctatctaa
     2581 gccatcttcc atctctgaaa caaaatcttt agcttgtgcg atttctaatg ctttccaaat
     2641 catttcatct gttgcatccg gcttaccata ctgcatattg tctcggatcg ttccagagaa
     2701 cagaactgct ttttgaggaa caaagcccat taattctcgc aaattatatt gagtcatgtc
     2761 actaatgtcc gttccattta tttggatcga accagattcg atgtcatata atcgagggat
     2821 caaattgact aaggtcgttt tacctgaacc agtcccccct atgattgcga cgatctcgcc
     2881 cgacttggct tggaaatcaa tatcttccaa tgcaagtttt tctgctccat gatatcgata
     2941 attgacatga ttgaaggcca atgttgcgtt ttttccagca aaagaaactg ttttaggatt
     3001 ttcagggtca tggataccga ttttttcatc caacacttca ttaatacggt cagctgaagc
     3061 ttgtgcacga ggcaccatga taaaaatagc ggaaagcatc ataaagctga tcaagatctg
     3121 catcgcataa gtcataaatg caatcagatt accgacttcc aaagtcatat ctgctatgta
     3181 gtgtccacca aaccaagtga ttgctacgtt tgtaccgctc atgatcaatg tcatcattgg
     3241 catcatcaat gcgacgatcg tattaacttt gattgccgta tttgtataat ctttattcgc
     3301 taaatcaaaa cgattttctt caaagcgtgt cttatcgaat gctcgaatca ctctgacccc
     3361 tgtcaatcct tctcgaaata ccaggtttaa acggtcagtt ttcttttgca tacttttgaa
     3421 taatggaaca gcaaagtaca tgattccgcc gatcaaaata atcattactg ggatgacaac
     3481 taagaagatt tttgtcaact gatgatcttt ttgataagca agtatgctcg cgccaatcaa
     3541 cgtgattggt gcattgatca tcatacgtaa aaacatttgt gtaaccattt ggatttgagt
     3601 cacatcattg gtcgttcttg tgatcaaaga ggccgttcca tatttatcaa acgcatcttt
     3661 tgtcagactc tctgattttt tatagacatc cgtacgcaat tgcttaccca gtttctgtga
     3721 ttcacgagta gcaaaaaatg tatttgcgat agcagctaag atactgatca aagaaaagcc
     3781 gatcatcaca aaacctgtat gccaaatata atcgacatct ccttgcgcta cccctttatc
     3841 aataatattg gaggtaagtg tcggcaaata taaatctgcc agtacttgga tcaccatgaa
     3901 aactgcagct gcaattgctg aagtcaagga gatccgtttt acgattttta gcatctagtt
     3961 cccttctttc tatttatata ctccgttttt cagccaagaa agtttcaaat tgaattcact
     4021 gattgcttgc tctactgagt aatcttcaga tgttgaaaat aagcgatacg aatgcgcaat
     4081 tgttgtaaat acagtattca tcactaattg gatcagtaat tccaattcat gatcattttt
     4141 tactttcagc ttcgtccgat cgatcaattc aagcaactct tttgcatctt gtttgtgttc
     4201 tttatgtgga tcgtgaggcg ttttttggcg actcgtatga ggagcgactt ttcttgaggc
     4261 acggtaatcc atgttcataa acaagttacg ataaaaagaa gcattctcgc cttccaaaat
     4321 ctcataaatc attttagaaa aataggattc gaatcctttg aatagatcac catcggattc
     4381 tttcattagt tgtaacatat ctttagagct gtcacgtctc attgtctcaa agtaataaaa
     4441 ataaagatcc tctttatctt caaaatactg atagaaactg cctctaggga tatccgctaa
     4501 tttgacgata ttagcaatcg aagcttcttt taagggtact ctagaaaact cgattctcgc
     4561 agcctcgatt agtctttttt gtttttcttt aggcaaatgg aaaaatgtct gtgtcggcat
     4621 atggattctc ctttctttca agatgacaca ttgtcatatg acgctatgtc attataacac
     4681 ctcctacctg ttttgcaaga catttgcata gtttttttca ttatcatccg atacattaaa
     4741 aaagtgataa gaggaatcct tccatttgaa ttaatgagag ttgcccatta aaatagccac
     4801 atactaaaca aaggatgaga taaatggccc aatcaattag tgtccaagac ttcaataaat
     4861 tgcctctcga tgctgacagc atcgttttag atacaagaaa taccttagat tacacaaaaa
     4921 accacttaag ccactcgctt tctatccccg ctgctatctt gccaagacaa ttaaaaaatc
     4981 tagatccaaa gaaactttat tatattctaa gctatacagg caggcgatca gaagcattag
     5041 ccgatcaact tactgctaat ggttttcgtg ctgtttatat cattggtggg atggaagctc
     5101 ttcagtcgag tgttgcttga atattgcttg ttagattcaa aagaggctgt gacaaaaaat
     5161 cgtgtcacag cctcttttat accctgtctt tttttagtta tttattcttc aaccttatca
     5221 atatgacttg atagcctttt ctttttcgag taaactgatt ttgttagtca tagttataac
     5281 aatcatagca ataaaaggag aaggtaagca tatggcatct gttttagtag tcaaaggtca
     5341 tcccctaact gcagaagaat cgcgcacagt taaggcattg acgtcttttt taacaagtta
     5401 taaagaaaat catccagatg atgaagtgac tgttttggaa ctttatcgcg atgatattcc
     5461 ggaaatcgat gaagaattat tatcaggttg ggaagcatta cgtgctggtg cagaattcac
     5521 ttccttatca gaaagtcaac aacaaaaaat tgctcgtttc aatgaattga ctgatcagtt
     5581 ccttgctgct gataaaatcg ttatcgcaaa cgccttatgg aatctaaata tcccaacaaa
     5641 attaaaagct tggttcgata ccgtcaatgt agctggaaaa acgttccgct atacagaaaa
     5701 cgggccagaa ggattagtga caggtaaaaa agcactgcac atccaatcta atggcggtgt
     5761 atacaacgga caagactttg cctctcaata tgtaaaaggt attttaaact ttgtaggcat
     5821 cgatcaagta gatcagctat tcatcgaagg aatcgattat gaccctgacc gtgcagatga
     5881 gttgatgcaa aatgcattag ataaagctgc tgctttaggt aaatcatttt aaaaattgag
     5941 ataaatgcat acaaaagaga cagaggaaaa aatcttcaaa gcagcatttg tgccttggat
     6001 attttcccat ctgtctcttt ttgttattct ttctgctttt tcaacggatt ttcttcataa
     6061 agagaagaga ttgttgtgta ccattcaaaa acatgagtaa tcactacttt agctgctgca
     6121 taaacaggaa tccccaagat cacaccgatg attccgaaca atttcccaga agtcagcaga
     6181 actaataaaa tcgttactgg atggattgct aactgattcc ctagtactaa tggagagatc
     6241 aagcgtcctt ctattgtctg ctctactgca aaaacgatca gtactttgat gatcatgaat
     6301 ggacctgcta caatggctaa aaagatagcc ggaatcatcg ctaaaaatga tcctaagtaa
     6361 gggatcaggt ttaaaaaacc cgcaataatc cccaacgtga cagcatactc taaaccaatg
     6421 accgcaaaac caatcataaa catgattgcc acagcaaaag ccacagttag ttgtcctctt
     6481 atataagaag aaacttgact attcatttca ctcagcacat tcaacgtcgg tttttgcatg
     6541 cgtgtcggta aaaatttgac aaaataaggt gctaattgtt taccgtcttt caatagataa
     6601 aataagataa acggcatcgt cagcagagcc actaaaatag tcgctaccgc gccgacaaaa
     6661 cttccaagac tctgaactgt actcttagat atatcttgga tcatatcgcc tgccgaattc
     6721 atgaacttat cacctgctgt ttccaactgt tcgcgaaatt ggttgaacag cgggtcccgc
     6781 aaaatctcgg ttaatttatt atcaatcgta tctacatatt gagggaagtt actgatgaag
     6841 ctcactgttt gttcctgaat cttagggatg atcactacgc tcccccatgc gattagtgca
     6901 acaaccaaaa caaacagccc aatgatactg taaagccttg gtacttttcg cttttcaaag
     6961 taatcaacta ccggattcat caagtaatac aagattcctg ctaaaatgat aggcagacct
     7021 acgattgcta aaaattgcca gattggtgca aacaaatatg aaacttttgt aaataaaaag
     7081 acgatcagca atattaacaa tacaacaagt aaggctgtga ctacttgatt atttaaaaac
     7141 cagcgccaaa accacgatac cggtctgttc ttcttatttt ttccttccat aattcctcct
     7201 gttagttata aaaaatttgg cttcccaaac caatagcagg cagtctatgc ggatgaagat
     7261 agattccatt tgcaatttgt ccttcttctg gacaagcatc aaaaatcaat gatacgtgag
     7321 ctactgtctt taaattccca ttggccatcg gaccgacata atcgatcgta tatacctgat
     7381 catcaaaagc aattgttccg cctgctttca aagaaaaagg ttcgtctttt gatacttctt
     7441 gaatcaccga atattttcgt aaggcagctg ttgctgtttt atcgaacaaa atcagcatgg
     7501 gttcttcctt gtttattgct tctgtcccaa tttctgtaat ctttgcctga atcattgcac
     7561 tctctccttt aattgctttc aaaaacagta taacacattc tcattcttta gccgtgtgga
     7621 ataattatgt gatagaataa aaacgaaaac tatttcatta caattaggag gaatcagctc
     7681 atatgatcga aaagataatt ttaggaacct atacgcgtcg cgaaagtaaa ggaatctata
     7741 cgatcgacct tgatacagaa aaagaagaat tagccaacct ttcattagct gttgaagaaa
     7801 atagccctac ctatgtagca aaaagtaaag ctggcaatct ttacacagtt actagtgtgg
     7861 atggattagg tggtgccggt gcgtacgata aggattttca ttttttgaat gcggtaacag
     7921 agtctggcgc tcctttgtgt tatgtagcgg ttgatgaagc acgtcaatta ctatacgggg
     7981 cgaactatca taaaggtgaa gtaaatgtct accatatatt aggagatggc gaactgaaag
     8041 cagcagattc aatattccat caagaagcga caggtcctca taaaaaccaa gatcatgcgc
     8101 atgtccacta tacggatctg actcctgacc aacgattagt cgtatgtgat cttggtacag
     8161 atcgtgtgta tacttatgat gtgtccgaaa acggcaaatt gaatcttgta actaccttca
     8221 ctgcagagcc aggaacaggc cctcgccatc ttgtctttca tccaaatgga caattcgctt
     8281 atttatttgg tgaattagac agcacagtcc gcgtgctttc ttataataaa gaaacaggtg
     8341 cttttgaaga attacagaaa atatcgacct tgccagaaga attcgacggt gaaaatggag
     8401 gggcagctat ccgcatttca aatgatggca agttccttta tgcttctaac cgaggacata
     8461 actcgattgc tgtattcgca gtttttgaag atggttccca cattcaaaac aagcaaatca
     8521 tctcaacaga aggggatttt ccccgcgatt tcgcactaga tccaagcaat aattttgtcg
     8581 tgtgtgccaa tcagaacacc gataatctga cattatatcg ccgtcatcct gaaacaggat
     8641 tactggaatt actgaaaaaa gatgtctatg taccagaatg tgtctgtgtt tattttgatc
     8701 gttagtaaga aaaggagaat gaaatgaatc agcaagacgt attagacaga ctacgggaag
     8761 aactaaatat tcctttcttt gatgggaaaa tcgaagataa agagtatagc gaagaggaat
     8821 atcagaaact caaagccgat ttacagaact actttgaaca atatgtacag aatgtagaaa
     8881 actaatcagc tttccatact atctgaaaag aacatcagga caagactttt ccgtctatcc
     8941 tgatgttctt ttctacaaat aaaaaaccgc cgagatggtt agactcgacg gaaaaggagt
     9001 atttactcga ttttgagtaa gtacaaggtt gttgttggtt ataggtatgt cattatcata
     9061 aaggattttg atcactctat cataaaggta atattacatt tatattagat aactctagtt
     9121 tttattgtta tcaaagattt cttttatttt ttcaacaaca aattagtcat tttttggatg
     9181 gcagctgtca attggtctgt cgcttggtct tgttttgctt cagatgtatt gaatagatct
     9241 tcatctaccc ctgattcaag atcgatactt tggatcattt cttcacagcc tgctacatat
     9301 gaaagataag ctttttcaaa atttttatga atgcccattg cttttgctgg tggacgcaaa
     9361 gtactgactt tcataagcat tgcccgatat ttttttgttc cttcttcaaa taacaggatg
     9421 atctcttcaa gagtcgattt gtccagttca cttgtttttc cttcatcaat tgcgtttctg
     9481 atcacttcat atttcggatg cattccttct ccgattgctt ctgtttcctt gacgatttga
     9541 tcgatggact gcacataata gccaatattc ggtaacatgt tcgttttcct actttctcca
     9601 ttgttgttat tatttattct acttgttttt atcctcaaat gacaagtact tgaaaaaatt
     9661 taatggtttc tatacagctc gccattctta ttcgtgctca aagaaaccat ctgctaaact
     9721 atattgtttc cctgcgacaa tgtcatattg gatcaattga tattcgtcgt aaatctcttg
     9781 ttgcgagcga ttgaattgcg cttctttata ccactgtccg ttttgatagt acagttctcg
     9841 ttcaaacgcc ggaagttcct gttctaatga taaaagcagc tgataaaagc catttgttgt
     9901 ctgattcgtc tgttccatta gattcgctgc aaaataaaac ggactggtga ttgcatcgtg
     9961 agtcgtttgc ttttctaatt ctcctttact gtcgaacata agaaattgtg tttcatgcaa
    10021 tgtttgtttg tcattttttg cttggatcgt atctgaatag atccctggaa gatgatcccc
    10081 ccaaaaaaca actaaagtcc ttctagaaag attcttcaat tcttccgtga actgcttcag
    10141 tgccgtgctg ctataggaaa tatcttgcag ataattctct aagtcaagtg tcccacttcc
    10201 atctgaagtt tttgcaggat agtctagatt atcatacttt ccatcgtagg gcatatgtgt
    10261 ctgcatcgtg actaaatgaa tgaactgagg tgttttttct tccttgagaa gtgtcattac
    10321 ttctttgtaa gctgactcat cagaaatata tggattattc tctatcgtat ccgtataggt
    10381 catcgtacgc tcgctgatga actggtcaaa tcctaatgtc tgataaacat cctctctttt
    10441 atacatcgaa gtattgtatg gatggatcgc tgtcgtctgg tatgattgtg cgtttaacgc
    10501 tgaaacgata gaaggaagct gatccatttt tggtacaagc atcgtgtaag gggtggtgag
    10561 ttgcgcatta aataacacca tagaaaaacc agtcaatgct tcaaattcga tatttgctgt
    10621 tccaccgcca tagttttgag aaagcatatt gccactatat gtttgatcag ctacttcata
    10681 ataatctgcc aatggctcac cagatacttc tacgccgttt aatcgactag gatctgagaa
    10741 actttcactc atcacgaaca cgatgttcgg cgactcttcc tctacagttt tatttttttc
    10801 atcggctagc ttctggtatt tttcagtgat ttctttgatc ttttctttcg aatatccttc
    10861 aggttcatcc attggttcta ccttcagatt gtacaaaaac ccaccgataa aaccagtgtt
    10921 atagtagttc attttctggc tgtaaggtat ccatagtgcc gttttattat atgcttttcg
    10981 caacaaatta tcgggattgt tgaaattact gatataccct agcagtccaa ttgccacaag
    11041 cagtgtcgtt aatcggatca attggaatcg ccttcctttt ttcaggcttc gaaacatgta
    11101 ccagcaaaac aatcctatca cggttcctgc ggcagctaag atcactgtaa acagcatagt
    11161 gccggtcata tctttcagca atccgatttc agtgatcatc ttcaaatcgt ctggataaat
    11221 cggttcttgc cgataactca ttttcatata atttgcaaaa cctaatacac cgatggctac
    11281 gacatacgta agggaaccta aagggacaga tcctgctagt gcaatcagaa aaataagaat
    11341 aatcagcaag acgagacagg ctaagaagaa tttttccgta tgccaagaaa aagcaaattt
    11401 caacgcaagg tctacggata gatcattttg acaccactgc aaaaataaat tactaaaaac
    11461 aacaagaaaa aagatactga tgattgctgc tgttcctttt aaaatctttt tctgtttggt
    11521 cgaaagtctg cttatatatc ttttcaaatg cttgctcctt catttctatc aataaaagaa
    11581 tgttgcttct cttattttag ttgtacgaaa ttcgaaagta aagaacggaa agaagaatta
    11641 atgaaatcca aatattctct ttctttatgg cctttctggt ttctttaata agcggacctt
    11701 tcgtactcca gaaatttttt ctagctgttg tttcacttga ttgagctgtt caaaatcagt
    11761 actgtctatg tcaatcaata tgtagacatg ctcattctta tggttacgcg atatatctgc
    11821 gatcgctagc tcgttctcat tcagcagaga aaatatctct gctaatatat tggttttctt
    11881 ttgataaaat atagtgaatc gaaacggcgc ttggaaaaac attcgtgcgg atggataatt
    11941 gaccgcttct ctaaccgtac cgaacaaaag ataatctttt aaggcttcaa ctgccaaacg
    12001 tccgctgtct gccaaagctt cctgagtcga tcccctagat gaggcaacat aagtatacgg
    12061 tcattttcta agaactcttc ttctggaaaa tcagtcaagt atttagccag atgttgtttt
    12121 tccaatgctt gcataacagc atatttatca acaatttcac ttcttcctac attgataagt
    12181 acagcatctt ttttcatttt ttctatattc gcctgatcga tcaatccttt tgtatcttct
    12241 gtcaacggca gcatgactac gatgtaatcc gaggtagaaa gaactgtttc taaatctgct
    12301 tgccgaacat aatccaactg ggcatcgcgg atagaataac ccaagacatc catccctaag
    12361 ctatagcaac ttagcgctac ttttgtgcct atcgctccaa gaccaagaag accgatggtt
    12421 ttgccttgca attctcgtcc cacataagca cttcttttgt tttctgcctg ttctaaaata
    12481 ttaggacctg ttagtgtttg taccatacga cttgcttcga ttatcggacg ggaagataag
    12541 agcaaacagc acaaaacaag ctcttttact gcattagcat ttacacctgg tgtattcatg
    12601 acgatcgtcc cgttttcaga agctttttca acgttgatcg tgttcacccc aacaccagct
    12661 cttgaaattg ctagcaaatc tggtgtaagc cattcatctt tcactttcgt actacgaata
    12721 actattcctt cggctggcaa atcatctatt attttgaaat attccccttg taacttttct
    12781 atctcttgtt ctggaatagc atcaatcaaa tgaacgtgat acataaaacc ccctccttac
    12841 taacaagtat ctcgaaattt ctgcaattag acaactgttt accatttgac ttcctagaag
    12901 caaaaagagc agttagagca gagtaggctg agtgataaaa ataaggctat gacaagttac
    12961 tgccataagc cttattttcg agagcaaaat attgagggtg agatatcttg ataaatggtg
    13021 ttccgttagc tgacattgat aataagacta aatttcaaaa aagttgaaaa gcaaatttcg
    13081 tcaatctctt cttatttctt aaaactaagt ggctttttca caacctcttt ttatctaata
    13141 accactgttt ccaactcaac taattttgcc cacgtttcaa tttgatctgt tgttagattt
    13201 agcgagacaa cggtatgatg accgccacct gcctgaatcc atgaatggac accttcatgg
    13261 aaattcggtt tagttttcca taacacacgt gcaacaggta aattaggtgc ttcagtcgtt
    13321 ggttcaaagg cttctacttc attgatcagc agtttataat gcgtaccgaa atctgccatc
    13381 gatacgacta ccccttctcc tgctttccca tcaaatacaa gacgcgccgg atcttctcta
    13441 tctcccattg ataaaggaga aacaacgatt cttggtttcg tcgcagcaag cgtcggatct
    13501 acttccatca tatgggattc caagattgct tcttggccac tagctagctc gtaagtataa
    13561 tcttccatga atcctgtatc tttccctcga gccatgattt tcagcaaccg atcaatcgct
    13621 gcagttttcc agtcgccttc cccagcaaaa ccgtatcctt ctgccatcaa acgttgtaca
    13681 gctagtccag gtagttgttt cattccatac aggtcttcaa aattagtcgt aaaagcgtta
    13741 tagcctcctt cttctaagaa acggcgaata ccgatttcct gctgagcttg tacttttaca
    13801 tgttcctccc atgtcttttc ttcataatca ccatagtcga aggtatacaa ttccttgtac
    13861 tcttcaaata attgatcgac ttcttcgtct gatacgcgat cgattacttg tacaagatct
    13921 ccgataccaa aataatcaac agtccaacca aattgaatct gtgcttccac tttgtctcct
    13981 tctgtgacac ccacgtttct catgttgtca ccaaaacgag ccacttttat tccaaaactt
    14041 tcattatagg ctactgcaac atccatccaa tcagcaatct ctttttggat ctcaggtcgt
    14101 ttccagtagc caacgacgat tttattttgt ttatttaatc gagcattgat gaacccatat
    14161 tcgcggtcac catgagcgga ttgatttaaa ttcatgaaat ccatatcgat cgttttccaa
    14221 ggaatgcttt cgttatattg cgtagctaaa tgaagcaatg gtttttgtag aagctttgtc
    14281 ccgcggatcc acatttttgc tggtgagaaa gtatgcatcc aagtgatcac acctgctact
    14341 tcttctcgat aattgacctc tttcatgatt tttgtgatcg tatcagcagt aactgctaat
    14401 tcttgaaggc ggattggata aggcaattgg ccattttcat tcagttcatc gaccatttct
    14461 tgtgcatgtt tctttacttc tcgcagtgct tcttccccat ataaatgctg tgatcctaca
    14521 acaaaccaaa attctttttc tccaatattc aacataatta gccactcctt ttatttgttt
    14581 tgtccgtaat acgcattttt tccgtgtttt cgtaaataat gtttatctaa aatccgttga
    14641 ggcagctctt ctgaaaatga attgagctgt ctggtaaata aattcatctt gcatacttca
    14701 tctagtacga ctgcgttcat taccgcactt tgcgcatctt tcccccaagt aaatggaccg
    14761 tgtccgtgta aaagtacccc tggaattgct aagggatcaa ttttgcgttc tttgaatgtt
    14821 tctatgataa cttttcctgt ttcatattcg tacccgctgt cgatttcctg ttgtgtcaaa
    14881 aatcgagcac aaggtaccga tccataaaaa gtatctgcat gcgtcgtccc catcgcagga
    14941 acatccaatc ctgcttgtgc ccagatcgtc gcccatgtcg agtgagtatg gacgattcct
    15001 ccgatttgag gaaatgaacg atataaaaca gcatgagtag gcgtatccga agagggattg
    15061 agtttacctt caatgatatg attgtccaag tccacaacga ccatgtcgtc tgctgtcatc
    15121 tcttcataac tgactccgct aggtttgata acgaaatatc gtgtttcagg atcaaaagcg
    15181 ctcacatttc cccatgtata tttgacgagt ccgtgtttag gcaagtcaag atttgcttgg
    15241 tatacttctt ctttcagttg ttccagcatt ttctatcact cctttttctt gcaattgatg
    15301 aatcgctgtt ttttcaatgt cgatcccctc acggtaacgt ctcataaata tctgatagcc
    15361 tgctatttct tctgctgaag gtgtgatggt gattccttgg tcttgtccaa acacgtcttc
    15421 tgccaagaag ttttctaaag acttcccatg tcgatccatc atataggctg ctaataacgc
    15481 gattccccac gctccacctt ctccggctgt gtccatcact gtaactggtg cctccatagc
    15541 agatgctaga atccgctgtc caacttctgg tgttttaaaa atccccccat gccccactaa
    15601 acgatcaatt tggacattct cagatttcaa tatatccatt ccgattctca ttgcaccaaa
    15661 agcactggct aaatggatcc gcatgaaatt cgctaaatca aaacggctac ctggtgtacg
    15721 tacaaataat ggccttcctt catttactcc tgtaatgttt tcaccggaaa aataaccata
    15781 agaaagtaat cccccgcaat ccgtatcgcc ttctagcgct ttgttgaata ataccgtgaa
    15841 taatttatct ctgcttatct ccatacccaa agaattggta aattcttcaa aaatactgac
    15901 ccaagcattg atatccgaag agcagttatt cgtatgaacc atgcctacta gttctcctga
    15961 aggtgtagtg accatgtcga tttccggatg gacattttct agttcttttt ctaatacgat
    16021 catagcgaag gcggaagtac cagccgaaat gttccctgtc cctttggcta cactgttcgt
    16081 agcgaccata ccggttcctg catccccttc tggcggacaa accgggattc ctggtctcaa
    16141 atttcccgta gggtcgatca gataagcccc tgattcgctt aacttgccag cttcttctcc
    16201 tgctactaga actttaggca aaatgtgatc caagtcaatc gctacgtgcg ctttttcgat
    16261 taactgacca aaaatatttt tcatttcttg atggtaggtt ttcgttgaag gatcgatagg
    16321 aaacatgcct gatgcatctc ctacgcctag tactttctca ccagtaagct gccaatggac
    16381 ataacctgca agcgttgtca aaaaattaat ttctttcaca tgttcttctt tattcaaaat
    16441 agattgatac aaatgagcga tactccatct ctgcggaata ttgtattgaa atttttccgt
    16501 taatattcgt tctgcttctt cagtgattgt atttcgccaa gtacgaaatg gtactagtaa
    16561 ctgttccttg ctatcaaatg ctagataacc gtgcatcatt gcactgaacc caatcgaccc
    16621 gattgtcgta agattctcgc catagatttc gaatacttct gctgccattt ttcggtaact
    16681 gacgcgcagt cctttccaaa tatcttctaa tgaataggtc cagataccat tttctagttt
    16741 gttttcccac tcataactac cagcggcaat aggagtaaaa gatgaatcaa tcaatacagc
    16801 tttgatccta gttgatccaa actcaatccc taaagatgta cgaccatcta tgacatctaa
    16861 catactttgc gcatttactt ccttcataat gatttaactc cctccttctc gtcaaacctg
    16921 atcaaaccgt ctttttagta gcgcttacaa tgattattgt atacttttgt acgtacatgt
    16981 caagcagtta tttacaagaa tacaataaaa aacacttgtt ttgtacgtac ataagttata
    17041 aaatgaaatt gcttacaaaa tctgatcaac cgccttctcg aaattctaga aggagagaat
    17101 acttatgaaa aaagaaaaaa aagtttcctc atcgtttatt tatttttttg gcgcatttgg
    17161 cggcattcta tttggatatg atattggtgt tatgaccggc gccttacctt ttttacagca
    17221 tgattggggt ttggcaggta aagcttcttt gataggatgg atcacgtctt cggtcatgtt
    17281 aggtgcgatc cttggcggct ctttatctgg tcttctttct gataagcttg gacgacgaaa
    17341 aatgattctt ctttctgcac ttatttttat ggctggttct gtcctttctg catctgcccc
    17401 tcacaatggt tcttattttc tgattgcagc aaggattctt ctaggtttag ctgttggcgc
    17461 tgcatctgcc ctagttcctg cttatatgtc tgaaatggct cctgctagac taaggggaag
    17521 attgtcaggg atcaatcaag tgatgattgc ttctggcatg ctcctttcct atgttgcaga
    17581 ttatttgcta aaagggttgc ctgaaacgat ggcttggcgc gtgatgttag ggctcgctgc
    17641 tgtccccgcg ctgattttat ttttcggtgt tttagctcta ccagagtcac cgcgttttct
    17701 tatgcaatct ggccgcttgg aagaggcaaa aagagtattg aactatattc ggacaccaaa
    17761 agaagctgaa caggaattcg aacaaatcca gctgaatgtc aaacaagaaa agacaaccgg
    17821 aacttcttgg catactttgt ttttagaaaa atatcgttcc ttagtcattg ccggtattgg
    17881 tgtagcagta ttccagcaat ttcaaggagc taatgctatt ttttactata ttccgctgat
    17941 cgtagaaaaa gcgactgggc atgcagcgag cgatgcattg atgtggccga tcatccaagg
    18001 aatcatttta gttgctggtt ctttgctctt tttggttatt gcagacaagt tcaatcggcg
    18061 aactttattg aaaataggcg gtagcgtgat gggactttct ttcatcctac cagctgtttt
    18121 aggaactgta ctggatgctc atacaaattc gttgttgatt ctcttgtttt tatgtattta
    18181 tgtcgcattt tattcttgca catgggctcc actgacatgg gtaatcgtag gcgaaatatt
    18241 cccactagct gtccgcgggc gagcatctgg cctagcctct tcttttaatt ggattggttc
    18301 tttcctagtt ggcttgcttt ttcctgtgat gactgcttct atgtctcaag aaatcgtttt
    18361 cggcattttt gggatcatct gctttttagg ggttttattt attcaagaga tcgtaccaga
    18421 aactcgtggg aaatctttag aggaaatcga acaaagtgca tctaaaaaaa cttatcctaa
    18481 acgtatttct atccatcatt cttaaagagg ttgtgacaga agtggtcagc ttcaagaaat
    18541 aagaaggaac cgcttagttt ccagtcacaa ggaacaattt caaaaaacag cttctcatgt
    18601 ttgatgaata ttgtgacttg tgaccagttg gtaggagaac accgtttagt ggagaggtgg
    18661 tgaaagaaac gttcagcctt gagcgatgac aaaagctctc acaaacattg cgacgagtaa
    18721 ccgtaggagc aataagaggc ggtaaaaacc cgtctcttat ttttcgtttt ctttcactgt
    18781 ttcccgttca actagtacag gttcatagag aatatttggc tgtgcctgtc ctttttcaat
    18841 cgttgtgacg atccaccgtg cggcatcttg tcccaacttt tcttttggat gcgtcaatgt
    18901 tgtcaatttt ttattcccta aatggcttaa agaagaatcg tctgttccta cgatcgatag
    18961 gtcatctgga atatgatagc cattttctgt caacttatct agcattaaac tagctatttg
    19021 atcgttatag caaatgatgc catctaaagc ttgttcttct ttcaaccgtt gtactgtttt
    19081 ttcagcgatt gtttctcgtg tttccgtggt gaaagtaatg atatcttccg tcaaaaaacg
    19141 cacattatac tgttcacacg cgctgataaa acctttcatc ctgtattttc cttgaagatc
    19201 gtcgattttt gtgatgaaca acagacgctg atgtcctttt tttatcaaat attctgctgc
    19261 cataaaaccg actttcacat catctaaaca aatataagag gcatttaact cttcataagc
    19321 agcattgatc atcaccatcg gaatgccttg ttctctcaaa gcaacataaa gtgctaagtt
    19381 tgggttatac tgattacttt tcgtaggctc aacgatcaaa ccatctaccc cataagcaat
    19441 cattttcttc aaacactctt tctccaattc gtgatcgtta ttcgtactgg ctaaaagcaa
    19501 tgaataccca gattctcgta attcattttc gattcctcgg ataatcgacg gaaaaatata
    19561 atcagagaga tatgtggtga tgacaccgat cgtttttgtt ctgttcaatg gtgttttttc
    19621 tttcttatag ctgtcatcta catatgtgcc cgaccctttt tctttacgca gatagccctc
    19681 atttaccaac acagcgattg cttgccgaat cgtatgtctg ctgacgtcat atatttcttg
    19741 cagcttgaat tctgagggaa tggttgtatt tacttcgtat tcctcacgaa ggatcttttg
    19801 ttttatctct tctgcaatat cttgatattt tgccatttgc cttgcccctt tttatttgta
    19861 cgtacatata tcataacgag tctgcaaggt ttattcaagc aaaaatcaag gtttcttgtg
    19921 ctcataaggc atacggaaca ggttcaccaa attctggaaa tccttgctca ttccacgtaa
    19981 agcattgcgc gttcgcgtgc cgattaggat tgtctaacgg atcccctttt tggttcttct
    20041 ctggtcgagc atgatagatc aaagtatcaa agcacccgtc ttctgaaacc gtaaacgagt
    20101 tgtgaccagg accaaattgc ttattttttt cagaagaaac gaatactggc tccgataatt
    20161 tatgccatga ttcttttaat aaaagattct tgtgctcatc agcccacagc aggcccatgg
    20221 catagttttc atctgttgca ctaccagaat acgtaatgaa tattttccca ttgcgagtaa
    20281 ggactgctgg accttcattt actgaaaaac cccgtttttc ccattcatac tcaggtatag
    20341 aaaggaggat ctgcttcccc tttaacgtcc aaggattatc catttcggat agataaagat
    20401 tagaattccc agtaatttca ggatcttgtt gggcccacac ataatatagc tttccttgag
    20461 aagagaaagt tgttccgtct aaactgaaag tttccctttt cgtttttatt tgtccctttt
    20521 ctatccaatc tgtattcatt ggatctatat gctgattttc aagtacgaac atgcggtgat
    20581 gatgagattc atcacgtata gttttatgat tgcttgcagc aaagtaaata taccattttc
    20641 cattgatata atggacttca ggtgcccaga tcaattcact catcggtcct tcttcatgag
    20701 cctgccatac aatcaggctt gcagcatgtt ctaattcatt cagtgacttt gcccttctta
    20761 attcaatcac ttggtagcct ggaaccgatc ctgtaaaata ataataacca tctgtatgtt
    20821 tatataccca aggatctgcc cgttgccgaa cgataggatt attataaagt gtcttcatca
    20881 taacaaattt cctcacattt tctttattct aattacattc caagagtatt tttctatccc
    20941 tacatttaat tcgtcatgtt tttctattga aaaatagttt atttcaggtt ttattttttc
    21001 tggttcttca cgtgtatttt tgtccaacag atcgctactg gttaatgtgt agtgctctcc
    21061 ttgatctagc aaattgaaat catgttcaat cgttatcttt tgttgttcat ttgtgcgatt
    21121 gatcaagaaa atagtcagct gattgccgtt atctacgatc acactgtcca cataaggaac
    21181 attttttatc ttttcacaat cataaacagg gcaaatgatt tgttgtttca atacttgacc
    21241 tttagcatat ttcgataagt gttgcatcac ataataagtt ggattcctcc atgcttgtcc
    21301 tttagcatct gtcagtatta aaggtattgt gtttactaac aatgattgac aactaatctt
    21361 tacgcgatct gcgtgtcgta aaatagccag ccctaatgaa ccaaataata atgtatcttc
    21421 catagtaaag taacaccaat cgatgggact accgacttca aaatccctat gtcttttttc
    21481 cggttgggca tgtacattcc attcatcaaa cgctagatac attgttttct cacttctcaa
    21541 atatcctttt acataatcac aagtagcaac gatttcttca atttgacgat cgaatcgata
    21601 agcttttgca aggtatgtcg ctgtatcatc acgctcattt ttaggaggtt tagtcaagtc
    21661 ttcttcatca tacttatcaa tatagttatg caaagctaaa taatctacat tctcataagc
    21721 ttcttctaat gcgatacgat cccattcagg gtacgatggt aaacgtggcg tagagcttcc
    21781 aactaagatt gtttcaattg aatcatccac taacttcatg acttttgagg cttcatttgc
    21841 taacctacca tattcataag cactcttagc ccccacttgc catggaccgt ctagctcatt
    21901 ccctaaacac cataatttaa cgccaaaagg ctttttttgc ccatttttta ttcttaaatc
    21961 actccagaag gttccttttg gaaaattaca atattctact aaattttttg cttcatcaat
    22021 tccttttgtt cctaaattca ccgtgaatat cggtttcgct tgtacatgac tgatccattt
    22081 aaaaaattcg tggagtccaa aagaatttgg ttctatacct cgccatgcta aatcaatttt
    22141 ttttggtcgt tgttctttcc tacctacagt atccacccaa tcataaccag atgtaaagtt
    22201 tcctccagga tagcgaatga cacctaattc taattccttt acataagaga ggacatcttg
    22261 tctaaacccg tcaccatcag ctgtttcatg gcccggttca taaatccctg tatagacgac
    22321 acttcccata tgctcaacaa atgagccaaa cagccgagaa ctgatctctc ctaccgtaaa
    22381 atcctctctg atttttactt tcacttcttc gtccaacagc tttccctacc tttcatttcc
    22441 tatagtcaag ataatagata caagtttcca tacgccacca gcaaagataa aaataaaagg
    22501 agggaaaaga aatatgagca gtacaaatcc taataacaat acgcaacaaa gaatatttct
    22561 caaaaaatca aaaaatactt tccatgcact ttgttcaaaa atttgtaaaa atgtgatatc
    22621 tttgttttca gaaatcacat atatccagga taaaagaaac atggaataaa taagtaaaac
    22681 tacatatcca ctaattgaaa gtacgattgt ctgttcttct aataattgat taacaagaaa
    22741 ataaataatg atcgttaaca gactaaggaa caagttctta ccaaaatgtt gctttattcc
    22801 ttgaaagaaa aaataaatga tatttcctga tccctcacct ttccattgcg ccattacata
    22861 aaaaacagca catgtagcag gcacgatagt aataacaggc aaacacacga ttacccagag
    22921 aatacctaat aagatgtaat cagaaaccac aatcaactgt ccataaagtt tagtctcaac
    22981 tattttcatc ttcctcactc tttcattcca gacatagctg ttccttgaac aaaatatttc
    23041 tgtgcaaaaa tataaaccac tagtaatggc agcattgaaa taaccgttcc agccattaca
    23101 ggtccataaa aagaaatgtt ctgccctgta aataaagcta accctgctgg taaagttctc
    23161 atttttgaac tacttgtcaa tatcaatgga taaagcaagt cattccaact attcatcagc
    23221 gtgaaaatcc ctaatgataa aagagtaggt ttgctcaatg gcaacatgat tttcaaaaaa
    23281 atattaaact cacttaatcc atcaatgcgt gctgcctctt ccaaagaatc tggtaaagta
    23341 ataaaaaagg aacgcatcat gaagatacca aatgcagttg tcatacgagg aatgatcaaa
    23401 cctgcataag tatcaagcat gccaagcaaa ttgacttcaa taaatagagg aatcatgaag
    23461 acttggaatg ggatcatcat tgtcagcaaa acgaaataga acaaaactga ttttcctttg
    23521 aaacgcatac gagcaaatgc ataccccgca agagaatcaa agaatacaga agtgatcact
    23581 gtccctccag caaaaatcac tgtatttttg atgtaatcta ataaagggat cgttgtccaa
    23641 accttaatat agttatctag ggtatagcta tctgcaaata ttctaggcgg aaatgagata
    23701 atgtcttttt cgtgtttaaa agaagaaata atcagccaca aaaaaggaaa cgcaataacc
    23761 aacaatataa ctagcatact aatcgttcga atgatttttt taccactaac tgcttttcga
    23821 ctattcacag taactgtaat cgagctttct ggtgccatac aggtttccct cctatttcat
    23881 ttgtttttct ttccgtgaca tgtacacatt agtggagata gctatagttg caacaatgat
    23941 aaacaaaacc gaagacagtg ctgatgcata gcccaattca taaggtgctg taaatcctcg
    24001 actgtaaata tattgaacag ctgtttctgt cttaaattgt gggccaccag cggttgcaac
    24061 atatacttgg tcaaacactt gcatcgaacc aatcagattc gttaaaatac aaaaacctaa
    24121 ggagggaacg atctgaggta gcgtaatgtt aaaaaactgc tgtcgatttg ttgcaccatc
    24181 catttgagca gattcataca aagagggaga gattccctga atagcagtca agagaatcgt
    24241 catagtaaca ccaaaattct tccaaacagt catgatggct accgtcggca tagcaagggt
    24301 tggatcgcga aaaaattgag ggatcggcaa gccgacttgt gaaaacaagt aaggaatcgc
    24361 ccctacattc ggatcaagca acatagaaaa aataatcgca attgacgtca atgaacaaat
    24421 aactggaatg taaaatgtcg aacgacaaaa acgattgaat ctggtatttt tagataatgc
    24481 tactgctgct aataaaccaa cgataatttg tgttggcgtt tcgagtacgg tgaaatataa
    24541 agaatgaatc aacgaattaa ctgctcgttc atcactaaat atacgtgtga aattttcaaa
    24601 acttacaaaa tttcttgatg taaagaaaat attcaaatct gtaaaactaa tagcaaatgt
    24661 tcctaaaagc ggtatgacag taaataataa taaaactaaa aaagatggag ttaaaaacaa
    24721 atacgcagca cgatcaggac gattccaata tttggtgaat ccgttcatat agtccacctc
    24781 atctttcatt ttgataaaat atacaatctt ggtttaaacg aatctgaata agatatccga
    24841 acaatcgctg tgatttttgc aataacacct atctgcaaat actaggtcga tcattcagat
    24901 atctgtatct tgccaacagt ctatcctgta taaagttctg tactattttt gcagtaattg
    24961 attgatggca tcatctgcat cgttcatgag tgtctcagga tcttctcctg ctaataattt
    25021 ttcaatcagt ggattgatga tatcgttatt gatcgcagag atttgggtaa aacctggtaa
    25081 atatggctct gcatagtcca tttgtttcga taattcagat acaataggat cattttttac
    25141 ttcttcatct tttgctactg aatccaagta ggcaggaaaa ccattttcga tactccattt
    25201 tttaccaatt tcagttgtat tccaatattt caaaaactca tagacagctt cttttttatt
    25261 attatctgta cttgccggaa tggcatatcc tacgccatct aaaatagctg tttttttgtc
    25321 tgaatcactc ccttgtggta aggtagtcac attataatca atctcgtttg ctttcaatcc
    25381 gttatttaac catggcccat tgatttcaat ggctagttga ccagcattca ttaaattgtc
    25441 catttcggcg ccagaaatcg attctggacc agtcttgtct acatggatca ttttttggat
    25501 cgcttttaac gttttaaggt tctcaggaga cgcaaattca gacttagtat aatcatcgtt
    25561 cactaattta ccgccatttg ctaacatcca gttatataac ggtgcagttc catccttatt
    25621 gaagacaaaa cctgatacat ttttactact atcagttaat tttggtgcca tttctgtcaa
    25681 ttcttcccaa gtagtaggtg gttgttcagt atctaatcca gcttgtttaa acaaatcatt
    25741 attccaatac atatacatgc cttgtacctg catggggaca aaatactgtt taccttctac
    25801 ttcacctaat tcaatagccg tttctttaaa atcagattta tctactccat catattccca
    25861 gaaatcgtct aatggttgta ccgctccgtt ttctacatac tgtgcaaaat ctggataatt
    25921 taatgcaata aagtcaggtg cttttttaga agaaatcgct gtaggaagtt tttcattcaa
    25981 attggcccaa gtcatgatat ccatttcaat tttgatgttt ttatcatttg ttttattaaa
    26041 ttcattaacg atttcttgca atatttcacc atcagatgca gtaaacccat tccaaaaagt
    26101 caaagtcact tctttatcag aatcagattc tgctgaactt ccgccaccgc aagctcctaa
    26161 aatacctgtt gcagctacca acaaagagcc aattaaccat tttttacctt tcattttttg
    26221 ttcctccttc atctgataaa cgacatctga ccacattcca agatagcggt ttcaataaac
    26281 aattgatttt cccatcttca gcttttacat ttgttgaatg acttggtttt acttgttcac
    26341 ttttaggaag attagttgct tttgtatcaa atccactcat ttcagaaaaa tcaatcactc
    26401 cctctaatga taattcagac aagtgaacag ttagattcat ttcttcttct ccacggttaa
    26461 ctgcaaatat gacaagttct ttgtcattta ttacggctat actttctaaa tacggcactt
    26521 cttcaaaatc ttccgtcgca tagtttgctg aacgaatttg tgggcttaac acctctcctt
    26581 gtccatacac agcagcttgc ataaatggat aatagatcgt ctgtctccaa gcttctccgt
    26641 ttgtttctgt cctgattggt gcaataacat ttaccaattg agcaagacaa gcaatcttta
    26701 ctctatctgc atgtttcaat aaagtgatta atagacagcc taatagtaat gcatcttcaa
    26761 aattatagtt atcttctagt aaaggaggag caatctgcca aggcgctgct tgctcatctt
    26821 gttccgctga atgataccaa atattccatt catcaaagga caggttgatc tgcttcttac
    26881 tatgtttctt cgccttgact gtatcgcaca tcgctgtcac ttcagaaatg aaccgatcca
    26941 tgtctaagga acgtgcaaga taatgagaaa gatcattttt ttggttgcca taatattgat
    27001 gcagagacaa ataatctatt tgatcgtacg cttgatcaag aactgttaac tcccaatcac
    27061 caaatgtagg catttgccga ttcgaacttc cgcataaaac cacctcaatt gaatcatcta
    27121 cgagtttcat cgccttagcc gtttcctctg ctaaccgtcc atattcttcc gctgttttat
    27181 gtccaatctg ccaaggaccg tccatttcat tccctaaaca ccatgtctta attccaaaag
    27241 ggctctcttg accattcgaa cgccttaaat cactccaata agttccttta ggaaaattac
    27301 aatattcgac taaattacga gctgcatcta ctccacgagt acccagattg acagccatat
    27361 tgatttcggc ccccacatgc tgacaccagt tataaaattc atgaatccca acttcattcg
    27421 tttcaattgt ccgccaagct aaatccaacc ttctaggtct atttgctatg ggcccaatac
    27481 tgtcttccca acgaaatcct gacacaaaat ttccaccagg atatctaatc atgggaatgt
    27541 tcaattcttt taccaagtga atgacgtctt ggcgaaatcc acttttatta gctgatggat
    27601 gatctggctg ataaatccct tcatatactg ctcgtcccat atgttcaata aatgaactgt
    27661 acaaccgcgg atcaactttg cctacacgct tgacactact aatcaacatt gtggcttcca
    27721 taactgacct cctttaattt atacatgaat aatacacctc gttgaaagcg ttgtcaataa
    27781 tataaatgtt aaacttactt attttttatc gattttaatt aaccaaaata ttaataatga
    27841 ttaaaacaaa taatttaata taacttttat taacaaatat gttaatatat aagaaaaacc
    27901 ttttaagagg tgatatatat gcaactacaa ataaacgaag aatccttacc ggtatatgaa
    27961 gcattagcta gtaaaacaag gatcaagatc atccagctac tttccaaaaa aaagatgaat
    28021 gtcaaagatt tggcaaaaga acttggagtg agcagtgcta tcacgacgat gcacgtgaaa
    28081 aagctagagg aagcaaacat catcaaaaca gaaaaagtag gacaacaaaa aatttcgagc
    28141 cttcgtgtcg ataaaatcga tatcagtttt ccagaaaaaa tattcaatgc ttttgatacg
    28201 aaagaaacat cgatacctat tggtcactat acaaattatg caattgaacc aacatgcggt
    28261 ttagctacga tccatgattt tatcgggaaa gtggatgaac cacgctattt tatggatcct
    28321 cgaaggatgg atgcccgcat tctctggttt accagcggat ttgtcgagta tcaggcacct
    28381 aattttttaa atacagagga tacattggag atgttggaag tttctgtgga aatttcttct
    28441 gaatttcctt tttcaaatga caattggcct tcagatatca ctttcagcct aaatggtgtg
    28501 gagctaggaa catggacgag ccctggagat tttgctgata tccgcggtaa atatacgcct
    28561 gattggtatc cagataatct taatcaatac ggcttgctga agacgatccg aattaccaaa
    28621 cacttgacga atatgaatgg agaacccttg tcaaacatca cgatcaacga catccctaaa
    28681 gagcaagaca cttggcattt gcgaatcgaa gtgaaagacg atgccaaaca tgtggggggc
    28741 tgtactttat ttggtaaagg attcggtaat tacgatcagg atatcaaatt aaaagtttac
    28801 tacagctgat atttttcttt tctattttat aaaacaaaaa cagccaatcc ctatgaaggg
    28861 acggctgttt ttgctttatt gttatttcca gaaatcatcc aaaacggaaa ttggcaaatg
    28921 tcgtttatgt tgtgtcttgt tccaccatgc ttcgattttt tcttgtgctt gagcgggaac
    28981 ttcttttcct tctagataat catcaatttc attatatgtg acacctagtg cgacttcatc
    29041 tgcaatcatt ggtttcccat cttcaagatc tgcagtcgga atttttgtgt ataatttttc
    29101 cggtgcattc aatgcttgaa gcaattgttt cccttgtcgt ttgtccaaac ggaataatgg
    29161 caaaatgtcg gctccaccat caccaaattt cgtgaagaat ccagtaatgt tttctgcagc
    29221 atgatctgtc cctaatacgg ctcctgctct ttctccagct acggcatatt gcgtaatcat
    29281 acgttgacgt gctttgatat ttcctttgtt aaaatcgctg attcccacac ctgcttcgct
    29341 taatacttgt acttgtgcat caacagctgg tttgatattt actcgcagac taacatcggg
    29401 ctggataaag tcaagagcag cttttgcatc ttcttcatct gcttgttcac cataaggaag
    29461 acgaacagcg ataaattgat agttttcatc ttttgtttct tcacgcattt cttccatcgt
    29521 tagttgcgcc aaacggcctg ctaaagtaga atcttggccg ccgctgatac ctagtacaaa
    29581 agttttaaga aacggatatt ttttcaaata tgctttcata aagtcaatac ttttacgtat
    29641 ttcttctttt ggatcaattg ttggtttgac acctaattca ttgataattt ctgtctgcag
    29701 gctcatttat tctcctccta tgcttctgta tgaagttctg ctacgctttt tttcattcgt
    29761 tccatgatag ccattttatg gttccagcat tcagtagaca gatcgactgg gtatttttgc
    29821 ggattcaagt ctcgtttata ctcttcccat agtgaatcta gattttcttt tgtatattct
    29881 ttgatttcgt ttagtgcagg taattcgtaa actctttttc cttcgataaa gatatcttgt
    29941 aaaactgggc gagctgtaaa gtctcgaaca gttttattga taaacgtatg cactggatgg
    30001 aacatgaaga tagcttcttc ttctcgaggg tcttcatccc ataatgtcac gtagtctcct
    30061 tcagatttcc catcaaaatt tcgagtgatg cgccacactt gttttttccc tggagtagtt
    30121 actttttctg cattgcttga taacttgatc gtatccatca tttttccttc atcatcttcg
    30181 atcgatacta gcttgaatac tgcaccaagg gctggctgat cataggctgt gatcaatttt
    30241 gtcccaacac cccatacatc aattttagct ttttgcatct ttaaattcaa aatcgttgct
    30301 tcatccaagt cattcgaagc atagattttt gcttccgtaa atccggctgc atctaattgt
    30361 tcccgaacac gcttagatat ataggccata tctccactgt cgatgcgtac gccttggaag
    30421 ttgattttgt cacctaattc tttcgccacg cgaatcgcac ttggcacacc tgatttcaac
    30481 gtatcataag tgtcgactaa gaacacgcag tctttatggg ttttagcgta ggccataaat
    30541 gcttcataat catttccata agattggact aacgaatgag catgggtccc gctagctggt
    30601 atcccaaata tcttcccagc acgtacattg cttgttgcat ctgctccgcc gatatatgcc
    30661 gctctagttc cccagactgc tgcatccagt tcttgtgccc gtctagttcc aaattccaat
    30721 agtggatctt ctccgatgac agatttgata cgagctgctt ttgtcgcgat caacgtttgg
    30781 aaattgacca tattcaacag tgctgtttct actaattggc agtgcgctaa aggcccttcc
    30841 acttggatta atggttcatt tgcaaaaact agttctcctt cacgagcaga acgaactgtt
    30901 gctttaaatt caaaattttg taaatactcc aaaaaacctt caggatagac ttctaatcca
    30961 cgcaagtaag caatatcgga atcattgaac gtcagattct ctaaataatt gactaaccgt
    31021 tccaatcctg caaaaaccgc gtatccgtgg ttaaaaggca tttctcgaaa gtaacattca
    31081 aaaacagcat gtaaatcagc tcgacctaat tcccagtatg tctgcatcat attgatttga
    31141 tatagatccg tatgcagcgt caaactgtcg tctgggtaag tattattcat cttgtattac
    31201 tcctcttggc aagttttcat ttcctaccca ttataacaaa aaaaattaat aggaaaagaa
    31261 atcttccatt ttttctgaaa aagcacatgt tttacggata tttcttgcta aaaatgaact
    31321 ccatctgcta aaactatcct ttcttattgg tatttaacct aaacgagaaa g
//
LOCUS       DAXWOO010000012        29881 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.012, whole genome shotgun sequence.
ACCESSION   DAXWOO010000012 DAXWOO010000000
VERSION     DAXWOO010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29881
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.012"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            316..1185
                     /locus_tag="ACGE70_000455"
     CDS             316..1185
                     /locus_tag="ACGE70_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFF8692121.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1394..2419
                     /locus_tag="ACGE70_000456"
     CDS             1394..2419
                     /locus_tag="ACGE70_000456"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFF8692122.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2458..3408
                     /gene="manA"
                     /locus_tag="ACGE70_000457"
     CDS             2458..3408
                     /gene="manA"
                     /locus_tag="ACGE70_000457"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFF8692123.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            3502..5205
                     /locus_tag="ACGE70_000458"
     CDS             3502..5205
                     /locus_tag="ACGE70_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFF8692124.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5207..7240
                     /locus_tag="ACGE70_000459"
     CDS             5207..7240
                     /locus_tag="ACGE70_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFF8692125.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7251..7691
                     /locus_tag="ACGE70_000460"
     CDS             7251..7691
                     /locus_tag="ACGE70_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFF8692126.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(7891..9018)
                     /locus_tag="ACGE70_000461"
     CDS             complement(7891..9018)
                     /locus_tag="ACGE70_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8692127.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(9021..9698)
                     /locus_tag="ACGE70_000462"
     CDS             complement(9021..9698)
                     /locus_tag="ACGE70_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFF8692128.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9962..11728
                     /locus_tag="ACGE70_000463"
     CDS             9962..11728
                     /locus_tag="ACGE70_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFF8692129.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11743..12180
                     /locus_tag="ACGE70_000464"
     CDS             11743..12180
                     /locus_tag="ACGE70_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFF8692130.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12193..13344
                     /locus_tag="ACGE70_000465"
     CDS             12193..13344
                     /locus_tag="ACGE70_000465"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFF8692131.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(13406..14104)
                     /locus_tag="ACGE70_000466"
     CDS             complement(13406..14104)
                     /locus_tag="ACGE70_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFF8692132.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(14097..15449)
                     /locus_tag="ACGE70_000467"
     CDS             complement(14097..15449)
                     /locus_tag="ACGE70_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFF8692133.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(15672..17072)
                     /locus_tag="ACGE70_000468"
     CDS             complement(15672..17072)
                     /locus_tag="ACGE70_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFF8692134.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            17233..17970
                     /locus_tag="ACGE70_000469"
     CDS             17233..17970
                     /locus_tag="ACGE70_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFF8692135.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            18095..18574
                     /locus_tag="ACGE70_000470"
     CDS             18095..18574
                     /locus_tag="ACGE70_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFF8692136.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            18571..19338
                     /locus_tag="ACGE70_000471"
     CDS             18571..19338
                     /locus_tag="ACGE70_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFF8692137.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            19568..20137
                     /gene="lepB"
                     /locus_tag="ACGE70_000472"
     CDS             19568..20137
                     /gene="lepB"
                     /locus_tag="ACGE70_000472"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFF8692138.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(20241..20843)
                     /locus_tag="ACGE70_000473"
     CDS             complement(20241..20843)
                     /locus_tag="ACGE70_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFF8692139.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            20949..21179
                     /locus_tag="ACGE70_000474"
     CDS             20949..21179
                     /locus_tag="ACGE70_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692140.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            21170..21418
                     /locus_tag="ACGE70_000475"
     CDS             21170..21418
                     /locus_tag="ACGE70_000475"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8692141.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            21524..22108
                     /locus_tag="ACGE70_000476"
     CDS             21524..22108
                     /locus_tag="ACGE70_000476"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFF8692142.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            22111..23184
                     /gene="prfA"
                     /locus_tag="ACGE70_000477"
     CDS             22111..23184
                     /gene="prfA"
                     /locus_tag="ACGE70_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFF8692143.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            23177..24016
                     /gene="prmC"
                     /locus_tag="ACGE70_000478"
     CDS             23177..24016
                     /gene="prmC"
                     /locus_tag="ACGE70_000478"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFF8692144.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            24009..24188
                     /locus_tag="ACGE70_000479"
     CDS             24009..24188
                     /locus_tag="ACGE70_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692145.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            24210..25235
                     /locus_tag="ACGE70_000480"
     CDS             24210..25235
                     /locus_tag="ACGE70_000480"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFF8692146.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            25410..26654
                     /gene="glyA"
                     /locus_tag="ACGE70_000481"
     CDS             25410..26654
                     /gene="glyA"
                     /locus_tag="ACGE70_000481"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFF8692147.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            26807..27754
                     /locus_tag="ACGE70_000482"
     CDS             26807..27754
                     /locus_tag="ACGE70_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFF8692148.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            27888..28517
                     /gene="upp"
                     /locus_tag="ACGE70_000483"
     CDS             27888..28517
                     /gene="upp"
                     /locus_tag="ACGE70_000483"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFF8692149.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(28656..28898)
                     /locus_tag="ACGE70_000484"
     CDS             complement(28656..28898)
                     /locus_tag="ACGE70_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692150.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            29019..29091
                     /locus_tag="ACGE70_000485"
     tRNA            29019..29091
                     /locus_tag="ACGE70_000485"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29052..29054,aa:Thr,seq:cgt)
     gene            complement(29210..29848)
                     /locus_tag="ACGE70_000486"
     CDS             complement(29210..29848)
                     /locus_tag="ACGE70_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFF8692151.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT        10157 a         5282 c         6075 g         8367 t
ORIGIN      
        1 atttttctta ctactcgaaa ctaagcgact ttttcacaac ctcttcctta aacggtgttc
       61 aaaagcttgc atctgcggta ataagcccaa ccaaacgaaa aataggagga gctatttttg
      121 tttggctgtt cttattactt gatgcagaac agctttttca caacctcttt ttataagagt
      181 ttttactcat taatttacaa aaaacaagca aaatgcgaat tttctcacat tttttttaac
      241 aaaatcaaag ttattgcttg aaagaagaag aatccatcgt taaaataaag cttgacgtag
      301 aggggagcaa gaaatatgaa agattttcaa ttgggggctg ccacctattt aaaaagtgta
      361 gccattcggg tgaaagaccg agataaaatg attgattttt acaagaacgt tgttggcttt
      421 gatttgaaaa gagaagaaaa cgaattagcc attttaggaa cagaagaagc tggaagtgaa
      481 atgttgctgc tggaagaaag tcctagagcg aatgatcaca tgggggtgat caaaaagctg
      541 aaccgttttt cattgattat ccctagcaca gaagaaatgg cagatatcct ttgtcgtatc
      601 cgtaaaatga attatccaat caaagaagct ttagaagatc atggacgtat ggggattttg
      661 ctcgtagacc cagaagagaa tgaattggaa atttaccatg aagagaataa gaacgaccaa
      721 agagaaattc cagaaacaat ggatcaagaa gcattattaa gaaaatctga agaaaaattc
      781 agtaaactat caacaggttc ttattttgaa aaaatccatt taaatgttac taacttaaca
      841 aaagaacgcc aatttcttaa tcatgtactt ggtttgaagg tacataatga agaagagggc
      901 atccatgtat tgaatgaagg agatttccat gttggtttga cagaagctag cggaggcgca
      961 attgatttgc ctacagatga agtgattgga cttgattttc tgaaattcat gatcgatact
     1021 gaaagcatgg aaggactgat tgctcacttg cagcaactat cagttgattt cttcttagac
     1081 aaaaagcaca cagtactcac aatctatgat gctataggaa tcgaatggtg gttcgtcagt
     1141 gagccaaaag aaaagaataa aactgacatt cgatgcgacg cttaactttt tctttcttaa
     1201 cgattgtcgc atgtctcgtc attttatgaa cggtcatact atcgcttatt ctagagatag
     1261 aaagagaaaa tagtataccg ggggaaactg cgcggttttt cgcgcagttt ttttgtgtaa
     1321 aaaaggttga ttcctcatag attaagcttt aagcttggac tgtaagaatc tgatagaaaa
     1381 gcaggagaaa aagatgtatc cacacacgaa acagcttatt ttgaaaaaga tggaagaagg
     1441 attttatcca ggtgtagcag tcgccttcat aaaagaaaat caagtagaaa catttacagc
     1501 cggctttgcc caagtcgttc ctgaaaagca gctgatgaca gaagatctgt tgtttgatgc
     1561 agcctcactg acgaaagtcg ttgcaaccac ttcattagtt ttacgtctat tagaagatgg
     1621 aaagatagaa ctcgatcaac cgcttcataa atatcttcct gaatttgaaa atcagacaat
     1681 cactttgcgt catttactga cacatacttc tgatatcaaa acatggatac caaacagaga
     1741 taagctatct caggaagaat tgatcgccgc ttatttgaag ctagaaccag gtgagcaact
     1801 aggaaaagaa gtaaaatata cagatgccgg tacgattctt cttgggttga tgctagagga
     1861 aatttaccaa gaaactgcag tggaaatctt tcaagaacaa gtattggaac cattaggaat
     1921 gatgaacagc ttattccttc cttttccttc caaaaaaatc gttcccacag aacaactgcc
     1981 ttcgggtgag gttttgcgtg gacaaactca tgatccgaaa gcaagagttt tggctgaaca
     2041 tgcaggtaac gcgggtctct ttattgattt gaaagacagc attacttttg tccaaatgta
     2101 tttgaacaag ggaaaaatcc gtaatggtcg ttttttagaa gaagagacga ttgattcttt
     2161 actgcaagat caaacaccga aaaaaaatgg ctatcgttct ttaggatggg acttaaaaaa
     2221 ggatgtagaa gacggtcatg cgattctttt tcatactggt tatacaggta cgtttttctt
     2281 gattgatata aaaacacaag aagcgttgat atttttgtca aatcgcgtac atcctgtcga
     2341 tcatcgtgcg gagtatctca tatgtcgaga tgaaattgtt gaaaactttt tgaaagagaa
     2401 agcggtatta ccgaaataat gctataatag ctatgaaaaa tgagaggagt ttgaattatg
     2461 caggaaccta tgtttttaaa accagttttt caagaaaaaa tttggggagg aagtcgttta
     2521 cgttcagtct ttggttttga tatccctaac gataaaatcg gagaagattg ggcgatcagt
     2581 gcgcatcctc atggtgtcag tgtagtagaa aatggcgaat tcaaagggaa aagattagat
     2641 gaactatggt cagaacacaa ggaattgttt ggccatcctt cagaaccagt gtttccatta
     2701 ttgatcaaga tcttagacgc agaagatgat ctatctgtac aagtacatcc agatgatgcc
     2761 tatgggatga aacatgaagg tgaattagga aaaactgaat gctggtatat catcgatgct
     2821 gaacctggtg cagaaatcat ctatggtcat catgcaaaga caagagaaga actagctgag
     2881 atgatcaaag atgggcgctg ggatgacctt ttgaagaaag taccagttaa aaaaggtgat
     2941 ttcttttatg taccaagcgg aacgatccat gctattggta aaggaatcat gattttagaa
     3001 acacagcaaa gttctgacac gacttaccgc gtgtatgact acgacagaaa agatgcgcaa
     3061 ggacaaacaa gagagctgca tatccaacaa tcgatcgatg taacaactgt ccctgcgaaa
     3121 acaccagaat tacaaatcaa agaagtccga aaagggaatt catcgatcgt cacttattta
     3181 gaaactgagt ttttcaatgt atatgagtgg gatattaaag gaatcacatc tttcaaaaaa
     3241 caagcccctt atacattgat gacggtaatc gaaggagcag gagaccttgt cgttgacgga
     3301 aaaacatatc cattagaaaa aggaacaagc tgtattattc caaatggcgt ttctgagtgg
     3361 gcggtccaag gtgaactagc aatcattgcg tctgaaccag gcaaatagtt cccagatagc
     3421 tgaaagcagc agacggtttt tcgtttgctg ctttttctac ataaaatccg tccatcaata
     3481 aatagaagtc tggtgaaaaa gatggaatac aaatggaaag caaacaacag cagtcgcgtg
     3541 tctatgaaac aactgagaaa agctggacat cacttaaatg aaatgatcat gccaaatcta
     3601 agtctgttta tcgcttgggg aatcttgtca ttagcaacaa aaggtgtgtc tggcgatttg
     3661 cacaccacat tagaagaggt atgccattgg atgatccagc tgcttttacc catcttgatc
     3721 agttattcag gcgggaaatc ggtaggggga caaaaagggg cagtcgccgg tgcaattgct
     3781 tctttagggt tgatcgctcg ttccaatgct ccacaaatcg taggagcgat gattatcggg
     3841 ccgcttgccg gcatcgtata tgaacaattt gtttctagat tccatgtgaa attcaaggct
     3901 ggatatgaaa tgcttgtcag caacctacta gtaggactga ctggtagcgt tatttgtata
     3961 attggaatcg tttttattga accagtttta aatagtgttc acttgttgct cgcctccgtt
     4021 gtgtcttggc tagtcacaat gaatctgtta ccacttgtca gtctgatctt agaacctcta
     4081 aaagtactat ttttcaataa tgcaatcaat catggtgtat tgacaccttt aggaatcgaa
     4141 ttctcacaaa cagtcgggta ctcttcctta tttttgatgg aggcaaatcc tggacctggt
     4201 ttagggatct tactttctat cctttgtttt gcagaaaaac aggaaaaagt aaatgcgagt
     4261 ggagcattga tgatccatgc gattggcggg attcacgaaa tctattttcc atttgtcttg
     4321 ctgcgaccgt atctattttt agctgtaatg gctggtgggg caagcggcac gttgatattt
     4381 caatggttag atacgggatt agctgcacct gtatcgcctg ggtcactact cgtgattttt
     4441 gccaatgcgc cttatccaca actagctggg gtaagtatag ggatagcaat cagtacattt
     4501 gtgaccttca tttgtgcagc gatactattg aaattaccaa ataaagaaaa agagaacaaa
     4561 acggtcaaag aaaggggaga aatcttggaa ccatctatca ttcattccgt catttttgct
     4621 tgcgactcag gtatgggatc aagtgctatg ggagccgccc tgctgaaaaa gaaattagca
     4681 gcagaaaaaa tcgatctttc tgtcggatac agctctatct atgaattgaa taaccaagta
     4741 aatcagctgg ttatcgtgca acatgaacta gcgggtcttg caagagaaaa agtacctcaa
     4801 agtcaaatct atgtactgga tcactttttg gaaacagata tcttgctgtc acgaataaaa
     4861 gaagcgatcc aatttccaaa gcgggaagaa aaaaaagaga tagcagatat cacagaacaa
     4921 aaaacgtcgc ctgctaatat tgtatttttg tataaaaaca acagacgcgg ctcacaaact
     4981 atgggcatga cgattttaag tcaattagca aaaaaagcga aaaaacagtt tgttatctca
     5041 aaagaatcaa tagaaaaaat caaaatccaa gaagaaacga tttatatcgc acaaagagaa
     5101 ctagctgtaa ctgaacagct agaagaaaag atccctcggc tagttctagt tgaacattgg
     5161 gtagctacga atgaatacga aaaactcgtg aagggagaaa tataagatgt atttatcgcc
     5221 cagagaaaaa caattattag cagaatttct cagtagcccg tcacctgtat ctatccaaaa
     5281 aatgatgaat ttattgaagg tcagtaaacg gactatttac cgcgaattag ataatcttga
     5341 agtctcattg aaatcggtaa atgcttcttt gaaaaaagca gctagaggct catttgtcat
     5401 tgaggcatca gaagaagtat tagagcaatt aaaagaaaat acagatggga aaagtaatga
     5461 cttatccact gctaaacgac aacacgcgat tttagccgag ttgttactga cgacagagcc
     5521 gttgagtctt gctcatttca tggaatcata tcgcatcagt aataccactt tttatgcaga
     5581 tatcaaacag ttggaagaaa gtattcgcca actgcctttg gaaatcattc ggaaccaggg
     5641 atacgagatc gaaggaccgg aaaaataccg ccggcttctg acagcgaatg tattagaatt
     5701 agaaatcaat gaatatgaac tttttcatag tatttctttc gatgcatcac tgaattattt
     5761 ttttcgattc gttgatccgc aacatctatc tcttgcacgc aaagtaatcg gggaagaatt
     5821 gatccaaaaa aagaccaatc taagtgatag aaagcttgaa catttggttt tgatgctgac
     5881 attgacgatg gagcgtgtga cgaaaaatca tttactgaca aatgagacat ataatagtct
     5941 ggcaaacaaa gaattactac agacagctaa acgattgttt tcacggatcg cttctgagac
     6001 aaaacaactt tatccagtaa atgagatcgt cttttttgca ggattattaa atgacttcac
     6061 gaactcattt gatcagaatt tctttgaaga gacgttcgat acacagcttg cttatttagt
     6121 aaaacaactg attcaagaag taagtgaaga gacgaatgtc catttttatg aagatgaaac
     6181 tttgtacaaa atgctgttga ctcatttatc tggtgtgttt tcaagagccg ttttacaaga
     6241 agagacactg accaatccga ttttagaaaa aatcatggtt caatacgagg aagttgcgaa
     6301 tgcaatccgt aaagcagtag agcggatttt tcaagaaaaa aaattatccg aagaagagat
     6361 cgcttatatg gtccttcatt ttgcgaattc attggaacga agtccgaaag aaagcaatat
     6421 cagtattgca ggattttcgc caagtggatt agcttctact agcatgttgg aactacgatt
     6481 gaaaaggtat tttcccttca tccaacaaat tcatttctat cggattgctg atttaaaaaa
     6541 aatcgattta caagaagaat atgatgttgt tatttctaca tctattttac caggatacgg
     6601 tggaaaatat attttagtct ccccgctact gctagaagat gaagtcaagc agctaaaaga
     6661 agctttttcg catattgaga agaaaaaaag gcaaaaaatt acgtataaca gcaataacga
     6721 gtggatgata gaagatactt atgaggaaac aatgcatttc atggatcaaa tcaatcaatt
     6781 actcggtcgt ttttttatcc aagaactgga taatcctgag acgatttccg aaacgattcg
     6841 cagcttattt ccttattttc ctgaaggact agtaacggat gaagaggtgg ttcatcgccg
     6901 gctgcttaag cgatatgaac aagcacctat cggtattcct catacaaata tggggttatt
     6961 ccatacatcc agctcattga tttctaaacc gatcttttgt attttcaatt tgaaacatcc
     7021 gttgaccatt gaagggatgg atagacagcc agtcgatttg tcccgtatgt tagttatgct
     7081 ggcgccttcg ccaatccaag aaaaagacgg gattctttta ggaaagatca gtggagcaat
     7141 catcatgaat gacttgaaca ccgagatctt ccgctcagga aataaagaaa tcgtttatca
     7201 gttgttagcg aaaatcctga ttgaagaaat aaagaaatga aggtgaaaag atgatacagc
     7261 taccagcaga aaaaatattg ctcgatcaat cctatcaaaa ttggaaagaa gcgttgaccg
     7321 agatcggcca gtggatgacc caacaagggg gaatcaaacc ggcatatatc caatcaatga
     7381 tcgaaagaca agaaaaagcg agtgtctata tcgggaattt cgtagccttg ccacatggga
     7441 ctgacaaaga tgaagaagta gtagaagaag gaatctattt agtccaagtg cctgatggtg
     7501 tggactttgg aacaaaagag gaaccaaaaa tcgcgacact cttatttgta gtggctttaa
     7561 aaaaagaaca tcagttagcg gtgctccaag atttagcctt tttctgttca gacatcgacc
     7621 aagtaatggc gctcagtgat gcggaaacca ttgaagaagt ccaaatgatt tttgaacatg
     7681 gacatatata accaatgttc acagtaaaaa aagctgcctt gcatcaagta ataacttcaa
     7741 ccaagataaa aataagagga gctatttttg ttgggaggtt cttattactg cggatgcaag
     7801 cttttgaaaa ctatttattc tagaaaaccg tttatttgaa ataggggacg gtgacaaggt
     7861 ttttgtcacc gcccctatta tttttgtgtt ttaatagctc ttttttatat agatagtgcc
     7921 catacctatg aataataaaa cgatactcca tataccaagt gtcaagatac cattcatgaa
     7981 agtaacctgt tctgaagcta gtggcgagaa aatatctcct cctatgatca cattttgaaa
     8041 atcaacataa gaagttggca agtaatgact gattgattct atccatccaa tcgaagaaaa
     8101 caagcgatac tctgctaaga aaagacaaag aagtgtacct aaaagattga ctagagaatg
     8161 ttcaaataaa gcagatagaa gaaaactgat tgtacttaaa aagagcaccc acaacaacag
     8221 gaacagaaag ctctggaaaa gatactcata catcggcagg attcgtatct cctgaccaat
     8281 gatcgttcct acaggataac ggaagtctcc caaaccattt ttgattatta ctagtataga
     8341 gacgatcaaa aatggcatca cgagacttag catagctgca ccataataaa tggtaaaacg
     8401 gatgctatgt ttctttacca tactatccgg taatagattg actagagaga ttacctgctt
     8461 tcttttttca taagagatga tggaagaaag aaacaaggcg gttatacata aaatcaaaaa
     8521 actagaaatc attcctgaaa aaatattttc gtagtaattt gctaaagaga gatgcgctgg
     8581 caagtcaaaa atagaatatc tttggatctg atgttctttc atataacgga gcaactcgac
     8641 acgtttttgt tgttcaatga ttggaattcc ttgtctttga ccagaaatca gtcgatccag
     8701 tatatctttt tcataatgat aagttgcttc gaccatttgc tggacattcc ctgtgttttt
     8761 tgcatgtaag attgcttcga tcagtttgtt gcttttctct aaactttcca caaattcttt
     8821 ctcattctca tcttctttca acgtttcaat caaacgttgc atctcttcat agttatgatt
     8881 cagttcatcg aattctgtat aagcaagttt atgatcaaaa aaaagtggga atgcaaagag
     8941 cacgaccaac aatagaggaa ataaaaagtt tactttgttt tttagtaaga gattgcgttc
     9001 aaatgtaagt aaagacatag ttacacctca tataatttac gataaatagc taaaatctct
     9061 tccatatttg aggattctgt gacgatttct ccatttttta gaaaacatgc tttagaacaa
     9121 atcgattgga tattcgcgat agcgtgggaa gaaagaagaa tcgtcttccc ctctttctgc
     9181 cattgtctga tgaggctatt caagatatca atacttcctg ggtctaatcc gttcaacggt
     9241 tcatcgaata acatgacaga tgaatcactg atcaagtaag cagcaagcaa gacatgttgt
     9301 ttcattccta gagacatttt tccaatcggc aaagtaaggt actcagtcat cttcaatcgt
     9361 tggaaaacag tttggcattc tactttgctc tgccaatagt atttgatcaa agtcaaataa
     9421 tcaacagccg atagatagtc ggataactga ttcgttgatt ctagaaaaaa gaaagaccgg
     9481 cgataatctg tataagcagt aggagagatg ttgtgaaggg acaattgatc ataagttgtt
     9541 ggcagcagtt gggcaatgcc attcagcaaa gttgttttgc caaatccgtt cggggcaatc
     9601 aaagcagcta cttctcctga ttctactaaa agattcaatt gtcgaatcac acgtttttta
     9661 ccacgtttca tgttcatgtt tttgatcagt aatgtcatag ataggcctcc aatataaaat
     9721 ttaataacat aatagaatgg aaaacatcta aattaaatgc taacaaaagt tattgtttgc
     9781 atattataaa aaaattaaaa taacagtttt atttaaaatt gaagttttct gataccgaaa
     9841 tctttttatt ttgctgacat actcaaacct ttggcacaaa gtttcgtgcc aaagtacgac
     9901 tatttctctt tttagtaagc ggatacaatt agagtatcaa aaggagagag ggagagacaa
     9961 aatggaacgg acgattccca aaaacaacat ttcgctaaaa gcgagggtac aaaaattagg
    10021 cagtacttta tcaagcatgg tcatgccaaa tattggagca ctgattgcct ggggtgtatt
    10081 aacggctcta tttatcccag atggttattt accaaatgag gctttcgcta ccatggtaag
    10141 cccaatgcta acttatctga ttcctttatt gatcggttat actggcggaa aagtaattgc
    10201 tggtgaccgc ggatcagttg ttggggcaat tgcaacgatg ggtgtgattg tcggaacaga
    10261 tattccaatg atgctaggcg cgatgatcat gggacctctt ggtggatatg ccatcaagaa
    10321 atttgatcag ctattccaaa aacggatcaa atccggtttt gaaatgctag tcaataattt
    10381 ttcagcaggt cttatcggtt ttggattagc gttattaggt ttttctgcga ttggtccagt
    10441 tgtcgatgca ttgactcaag caatggcaaa aggtgtagag atcattttat ctgcccattt
    10501 gattccattg acaagtattt ttattgaacc agcaaaaatc ttatttttga ataatgcaat
    10561 caatcatggg attttgacac cattgggaac tgaacaagta gcagaggctg gaaaatctat
    10621 tctattcttg cttgaagcaa atcctggtcc aggacttggc gtattacttg cttttacttt
    10681 gtttggcaaa ggagcagcaa aatcttcagc accaggagcc atgatcattc actttttagg
    10741 cgggatccat gaaatctatt ttccttacgt tatgatgaaa ccgttattat tcttatcagt
    10801 tataggcgga ggaatgtcag gaagtttcat tttccaattg atggatgctg gactaagagc
    10861 gccagcttct ccagggtcga tcattgcgat tctagcgatg acaccgttca attcttacct
    10921 tcctgttatt ttgggcgtga ttgttgccgc agctgtttcg tttggtattt ctgcagtaat
    10981 tttgaaagcg gatgcgaaag atacaaatga agacttcgaa aaatctgttc aagagacaaa
    11041 ggcagctaag caagcttcca aaggtgccca aggtattcct caaggagcta cattctctgg
    11101 cgtacaaaaa atcatttttg cttgtgatgc aggaatggga tcaagtgcga tgggcgcttc
    11161 gatcttgaga aaaaaagtac aggaagcaga cttgcctcaa acggtgacca atcaagcaat
    11221 cagccaactg acagatgact cgaatacatt gatcgttact caggtcgaac tacaagaaag
    11281 agcaaaacaa aaagcaccga gcgcgcgttt tgtctcagta gaaaactttt tgaattcacc
    11341 tcgatatgat gaaatcattc aagaattaag cagcaagcag aaaacatcag tttctgaaat
    11401 tgagaatgag ccagcagaag agtttctttc agcaaacatc aaacaaatca aagagattct
    11461 acttgtccac gatgatcgtt ccggatcagc aacaatgggg atgaccgtgc tcaaagatat
    11521 attagacaag aaccatttaa ctatcccgtt aagaaaagtc aatatccaag atttaaaaga
    11581 tgaacctgat tcactgatcg tagccaaaga aagtttagcg gaaaaagtac agatggctgc
    11641 acctcaatcc attcatttat cagtcaacag catggtcaat ccgcaaaaat acgaatcgat
    11701 tgcacaatat ttgaaacaga cagcttaagg gaggaaaaca gcatgcaaag tttgccattc
    11761 gacatgatcg agctcaatcg atcttttaaa accaaagaag aagcgatccg ctattgcggt
    11821 cagatgcttg tagaagcagg gtgcgttgat gaggaatata tcgatgccat ggtagataga
    11881 gacaaaatgt tatctgttta tatggggaat tttatcgcga ttcctcatgg aacagatgaa
    11941 gcaaaaaaat atgtgaaaaa atccggcatc tgtgtcgttc aagtaccaga tggtgtagat
    12001 tttggtaccg aagaagaaga aaagattgcg actgttttgt tcggtattgc aggagtcgga
    12061 gatgagcatt tgcaacttgt acagcaaatc gcactttatt gcagtgatat ggacaatgtt
    12121 gtccagctag cagacgcgct cagcaaagaa gaagtaacgg aaaatctagc aattgcttaa
    12181 ggagagagaa aaatgagagc agtacatttt ggtgcaggaa atatcggtcg aggatttatt
    12241 ggagaggttt tggcagctaa tggatttgag attactttcg tagatgtgaa tcacacgatc
    12301 attgatgctt taaatgaaag aaaagaatac gaaatcgaat tagcagatga atcaaaagaa
    12361 aaaatcactg taaaaaatgt caaaggaatc aacaatcaaa caaatccaga aaaagtaatc
    12421 caagaaatcg ctgaagcaga tttagttact accgccattg gcccgaatat cttacctttt
    12481 attgctgagt tgattgcaaa aggtatccaa gaaagagaaa aggaaggaag cacaacatct
    12541 ttagatatca ttgcctgtga aaatatgatt ggcggttcag catttctaga aaaagaagtg
    12601 tataaatatc ttccggaaac atcatttact gacaaatata tcggctttcc aaatgcagca
    12661 gtcgatcgaa tcgtcccatt acaacaccat gaagatcctt tattcgtcca agtggaacct
    12721 ttcaaagaat gggtcattga cgatagccaa agaaaaaata aaaaaatcca attaaaaggg
    12781 gttttatatg tagatgatct agaaccatat atcgaacgca aactatttag tgtcaatact
    12841 ggtcatgcga ctgtcgctta tacaggtgcc ttgttaggtt accagaccat tgatgaagca
    12901 atgcaagatg cgttagttgt ggcacaatta aaatcagttt tgcaggaaac aggaagtttg
    12961 ctgattgcca aatgggggtt tgatgcagag cagcatcatg cgtatattga aaaaatcatt
    13021 catcgcttcc aaaacaaaaa tatctctgat gcgatcacac gtgtcgctcg gacaccatta
    13081 cgtaagttag gttatcaaga aagatttaca cgtccagtga gagaactaca agaacataac
    13141 ttgacttgcc cacatttgac ggcaacaatg gggattattt ttaattacta tgatcctgaa
    13201 gatgaacaaa gccgtcaatt gcatgaaatg aagatccatg aaaacttgga acaattgatt
    13261 caagaagtca caggaatcaa tgatccgaaa acaatcggga atatcaagca aaacgtgaac
    13321 cgttatgcca agcaagtcgc ttaaaatagg atacgaaaaa ggggaatgtg acgaaatttt
    13381 tgtcacattc ccctttttcg aataactagg ctgtttgtcc ttcaagatag ggcagatcca
    13441 attctttacc gtaacgttgt tctaaagcta ggcggatcaa acgagctgcc aggttctcat
    13501 ttcttgcaag gataggtcca tggaaataag aaccgaagac atttcggtaa atgacacctt
    13561 cagattggtc ttcaccatta tttccttgac cttgtaccac tttccccaaa ggtcgttcac
    13621 cttcacctaa gaaagtccga ccattgtggt tttcaaatcc ataataagtt tcattgaatt
    13681 cttcgttatg gataacgata tcaccgataa accggttatt ttcctgactt aacgtgtagt
    13741 gatccagtgc actgattcct tggatctttt ctccattagc tcccatgtag tagtgaccta
    13801 gcaattggta gccaccacaa atggctaaca tgacgccatc attttcgata tagttgatca
    13861 aagaatcttt ctttttctgg atgtcttttg aaatgattaa ttgctcaaag tcttgaccac
    13921 cgccaaagaa aaccaagtca tatttatcag catcaaaagg ttcatgtata ctaacgatct
    13981 ccgtatggca agaaaccccc attttttctg ctatatattt caacatcaaa aggtttccgt
    14041 tatcaccata tgtgttcatc aaatttccat aaaggtgggc gatattgagt tcataattag
    14101 ccacggttca ttcctccttt gataaatcct tgagcagcta gttctttacg aagctgcaat
    14161 acagcagtgt aagtagctaa aatatagaca tgctcagttg gcaattcttt gattttttcg
    14221 attacttctg gtaattcttt tgtttctgtc aatttatttt ctggaatccc ggcaactttt
    14281 agacgtaaag tcatatctgt atgacggtct cctccagaaa taacagctgg gatatccata
    14341 tcggcaaagt tttcaaaatt accatcccaa atccagctga catcgattcc gtctgcgtaa
    14401 ttggcattta ataaagccac taaagaaaat ggataaggag ctagaccaat catatcgatt
    14461 acttgattga tacctacagg atttttgaca agtactaatg tacatttttt cccattgata
    14521 ttgatgactt cttgtcggcc gaatactttc tcgtcatatc ctaaaccttg tttgatttta
    14581 tcaggatcga cattgaagta ctctgccact gcagtagctg ctaaagcatt gtagacatta
    14641 tacataccgc caacttcgat tccgtattca tgaccatcga tcacgaaatc tgctgacaca
    14701 ttgtccatcc gtaccatatc agttaatcga taatccaatt caggacgttt gaagtcacaa
    14761 tttggacagt aatatttccc taagtttgca taagtgatca ttttataatg caagatgtga
    14821 tgacaatttg gacataagac accgtctgta ttgtaatgag ccatttgttc acgatcttct
    14881 tcatggtcaa agccataata ttttctagga ttgactgttt cgatagaatt gaagattggt
    14941 gaatcaccgt tactgataat cgttgcattt ggtgactttg cggcaccgtc tacaatcatt
    15001 ttataggtcg tatagatttc tccataccga tccatttgat cacggaaaat atttgtaaat
    15061 aagaacagtt ctggtttcat atattctgtg actttgctca aacttgcttc atctatttct
    15121 aggacagcaa actttttctg tccttttttt ggttttgcct gcaagaaagt cgatacaatt
    15181 ccctgtacca tattcgcacc agtagggttg gttagaactt catcgaattg ctggcgcaaa
    15241 atattgactg ttaaagcggt agtcaatgtt ttaccgtttg ttccggtgac aacgacgact
    15301 tggtagtctt ttgccaaact atctagtata tgtggatcga ctttcagcgc tagtttacct
    15361 ggtaaacttg atccgccttt aaaaaaagtt tttaatgccc attgcgacgt ttttccaact
    15421 gcaatcgcaa aatgactgcg tattcccatt atgtaacctc caaaaacgat ttgctgacga
    15481 ataaattcaa ctaatcatac catatgttga aaaaaaggtt aaattgcttt tcttattctt
    15541 aagaaaaggt tacggctgaa agtgcatgaa atcggacaaa atcgcatgat ttaacaaaaa
    15601 aacagcttga aacaaaatga ttgtttcaag ctgctgtgat tttattattc cctcttacct
    15661 atattcccat tttaaccgat aataattttc tcgcttggat attcatagcc tagatctcgt
    15721 gtttcttttg gcacgaataa catcaatgta tatagcattc cgatccgccc aataaacatc
    15781 agtgtagcaa tcgtgatttt gcccacaaca gacaagtctc cggtaatccc taaagataaa
    15841 ccagttgttc caaacgcaga agttacttcg atgatgatcg agatcagcgt ttgttcttct
    15901 gtagcagata aaaaaacgat acaaaagaaa cacataccaa gagaaagcat aaagacaacg
    15961 actgattttc gcacatcatc ttgatcgatt ctccgtccaa aaatattgat gttatcttca
    16021 cttttgagga aggaatatag atacaaaccg atgattgcta cagtagttgt tcgaatcccg
    16081 ccgcctacag aactaggact gcatccgata aacatcagta aagaaaaaat gatcagtgtt
    16141 gtgatttgaa aatcacccaa atcgtgaatc tgcagaccag cattacgagt cgtaatcgaa
    16201 taaaacatcg aattgatcca tttgacactc atattggaat cttggaataa atggtccttt
    16261 tccaatagat aaatcaagac agttcccgaa acaaacaaga taacaaaagc taacacagct
    16321 aatttggtaa acaaggaaaa acgaaacgga agcttcgcat tggaacgttt gtataatagc
    16381 cattcccggc actccatcaa tacaggaaaa ccaattcctc cgacaaaaat caaaaacatg
    16441 atcaggatca agaaaaaata gtcatgtgca aagggcttga tggaatcccc agtgacgtca
    16501 aacccagagt tcgtgacagc cgatatcgcc tgataaaaac cgtaaaaaac ggcatcacgc
    16561 caacgatcaa aatagcctct gtaataaaaa taaatagaaa agaatgtccc aaaaagtaat
    16621 tgaaaccaga ttaaaatagc aaaagtgatc cgaatcagtc ggacgatacc agacaaacgc
    16681 ggttgattca tatccgtcat gatcagctgt cgctgtttca gtgtgatccg tcgtttagaa
    16741 aaaatgacaa aagcagtaga aatcatcatg atccccaaac caccaacttg aaataatatt
    16801 tccagcagta taataccgtt atcattgaac acagaattga tatcaaaagt cgatagtcca
    16861 gtcacactca ctgtactgat tgccatgaac agcatgtcca caaaagaaac atggctatct
    16921 ggttcgcgaa aaatcggcat ataaaataga acgagtgata agaccgtcat caatatataa
    16981 taaaaaacga tgatttggat cgatgagaaa tgattggcag caaacttctg tcctcgccgc
    17041 tgcagccttc gaaagaataa atcaacatgc acagtactat cctcctgaca atagtcgttt
    17101 ctaccagtat acaaggggaa ttaggaatac tcaaagaaat tatcaaacag ataacaatac
    17161 agctttcgat taaaatagaa atggcttata ataaggaaaa tcctagaaaa aagaaggagg
    17221 aggaataaca aattgattaa tgctcaacaa atcgtagaaa aaatgaaaaa tcaaaaaata
    17281 aattatgata cagttctgaa aaaaatgatc atgcaatggg agaaagaaga agtaaagcct
    17341 aagatattaa tacatacatg ctgtgctcct tgcagtacgc acacattgga attcatgtct
    17401 caatatgcag agatcacgtt atttttttca aattcgaata ttcatcctaa aagtgagtat
    17461 ttgcgaagat tacatgaaca aaaacgattt gtagaccaat ttaatgagaa aactgaccat
    17521 catgttgcgc taatcgttga tgaataccgt ccaaatgaat tcaccaaaat ggttttaaca
    17581 aaccatcttg aaaacgaaaa agaaggtggt gctcgctgca gtgcatgctt caatatgcga
    17641 ttagatcttg ttgcgcaaaa agcgcaagaa ctaggatacg attactttgg aagtgccctg
    17701 accatctcgc caaagaaaaa tgcagctttg atcaatcaga tcggaatgga tatccaaaag
    17761 atttacggga cacaatactt gccaagtgat ttcaagaaaa acaaaggcta tgaacgctct
    17821 ttgcaaatgt gcaaagagta tgatatttat cgccaatgtt attgtggttg cgtgttcgct
    17881 gccaagcagc aaggatgcga tctgaaagaa atcaatcggg aagcaaaagc ttacttaaaa
    17941 acatcaaccg tagtttttga ggaaaattaa agaaatcttt cggaaaaacg taagaaaaaa
    18001 ataaagaatt tatggtagaa ataaggtaga ctgtataatt tgtccgtagt aaagtaagga
    18061 aacaagaata aatttcaatg aaggaagaga taagatgcga aaagtagcac ctattttgaa
    18121 gccaatcgct gtattaacaa tctcgacgat tgtcgcgact actacaggta ttgtgaatag
    18181 ttccagctac cgtgcaccaa gttcaagttc tagtactgta gaagaaacaa caccaactac
    18241 ttcagtctcg acatctgaac caacaacaac ggaaagtttg aatgaaacca ctacgacaag
    18301 tacatgggac gatacaacaa ctagttcgtc gtatgatgag acatatacaa ctgacagctc
    18361 aagcgaaaca agtaatacgg taccagatgg cactccatcg gattcaacca atccaagtac
    18421 aagtccggat acaagtgcta gctccgaaac gataccaagc agtacgactg tagatgataa
    18481 cacgcaaaat gatgatgcag cacgtacagc acagcaaaac caagattcag gaaataccca
    18541 aacgtataac cagcaagcag gtgattctca atgacatttt tactagacct tatcaacggg
    18601 gtgcataaat tccttggcta tttagacatc agtcctaaat atcttaaccg tggatatacg
    18661 attttaagtg tgattcccac gctttattta ttgagaattg tttatggact ttggcaaaac
    18721 caaaactatc ttcaattttt cttatatgga cttgcatttc tagtgcttgt ttattttact
    18781 gttttaaatg tattttatta ctttttagat aaaaacacga aagccgatgt cactcagctt
    18841 tttgtgaagt atttacctga tgaagcgttc aacatccaga cagaagaatc gatagataat
    18901 ggaaattcga tcgataaagt gaatacacaa gaggtcatgg tttcttatga cgaagattat
    18961 caattgaaat tagccgagaa tatgcggtat ttgattggaa atggtgagat caaaacaaat
    19021 gaactcggtc gcatggatgg atttctggtc gatagaaata cattgtatcc ttattactat
    19081 gtcaagaaaa caggagagaa agagtatgtt ctaaatattg gacgaagcta ctcggacatg
    19141 gaaaaagtag ggaccattcg acttgactca acagaagaca cattggaacc tgtcggattg
    19201 ttcatcgtcg gcggtgattt tgtcaaagac gggttacgct atcatgagcc ttatcgtctg
    19261 aaattgattg caaaaaaggc acagtcgcag gaagaaccag tggtttatag tcgtagccaa
    19321 agaagaagac attcataatg tgatgaaacg ccgtgtatca gtggttgcga taaaaacgtt
    19381 tattcggaat aagggggatg tgacacgtct tttgtcacgt tcctttttta tataacgaga
    19441 aagataagac gaacacatca cttattcttt cctttcattt ataaacattg tagaataaag
    19501 aaagtacaac gaagtattgt gaaaaaacgt aataaataac gaggaaaata caagtaggag
    19561 gtaagctgtg aaagaatttt tgaaaaattg ggggattttg atccttgtat tagcggctat
    19621 tttattagct agagtgtacg tgtttacacc agttactgta aacgggcatt cgatggaccc
    19681 tacattaagt gatggacaac gattgatttc atctaagatc tcaaactatg aacgaatgga
    19741 tattattact acaaaggaac cgggggatga agaacggatg attgttaaac ggatcatcgg
    19801 tatgcctgga gatacagtca agatggaaaa tgaccagttg accattaatg gcaaaaagta
    19861 tgatgaacct tatctggatg agttcaaaaa agaattttca gaagataagt tacaaggaga
    19921 atatgcttat agctcaggat tccaagcaca agcagaaagt tcatccacct ttacgagtga
    19981 ctttgaatat actgtgccaa aaggaaagta tcttgttcta ggcgataacc gcttgatttc
    20041 aaaagacagc cgaatgtttg ggttagtcga caaagatatg atccaaggaa aagttgtttt
    20101 ccgttattgg cctctttcag aaattaaaat catgtgatga aaaaataaaa agaagctgtg
    20161 acatgttttt tgtcacagct tctttttccg aaaattcctt cttatttctt ggagttgctc
    20221 actttttttg caatctcatt ttaagctttt tggctgatgg ataagaaaag cgaagcgttt
    20281 ttgttcataa atagcttttc cccacgatgg aaggtatgac tagtagcaga gacaaagccc
    20341 gttttttcca atcgattatt caattcttct tggctaaagc cattatggac tttcggatga
    20401 ctaatttgtt cattcttgtc aaagtctacg atgatcaatt gaccgccagg agctaaaatt
    20461 tcataaagtt ttgtaagaat attctccgta tcaggaatgt gtaaaagtac aagtgataga
    20521 aggatgagat tagcttggac agcaggaaat tcgacagaag catcagcgtg gatcgttcgg
    20581 acatttttca gatctgctgc ttggatcttt tcttctgcca ttttcagcat agtttctgaa
    20641 gcatcggcaa tgatcagttc tttgaatctt tccgcaagcg gtaaactaac aagcccagtt
    20701 ccgcctccgt aatccagcag gactttttgg tggctgtcgc gcggtagata agtagttagt
    20761 tcttcgcgaa taatcttggc taatgcttga cggtcagggg aatcataatg gttagcgatg
    20821 gaatcaaaaa tattttttgt catcaaatag ctccttacgt aaaactttga aattttaact
    20881 tattatagca cgtgaaaaga aagaaagcat gctatactca attcaaaaag ggagaatcta
    20941 gaaagaatat gccgatatcg tttgtaaaag atagagagga aaaaggaaag tgtgtccgag
    21001 aaatattact agacttgcct gagtggtttg ggcttcctga atcaacggaa aaatatatcg
    21061 aggaatcaag taaactacct ttatggtgtg aaaaaagaaa agaagaatat ttaggcttta
    21121 tcactctttc acagacaagt gaagatactg ccgaaatcta ttctattgta tgggaataaa
    21181 aaaagactat catcaccaag gattaggaac aaaacttttt aaagaagcag aaggttacgc
    21241 agcaaaacat tacaaatatt tgcaagtgaa gactgtagac gaggggcact actctatata
    21301 tgatcaaact atttgtttct acgaatcatt aggattttca aggttggaag tttttccaaa
    21361 tttatgggat gaatggaacc catgtttagt tcttgtcaaa aaattggaac aaaagtgagc
    21421 aatagattta atactaatct gtgcttgatg aaaattctca ctgtaaaaaa tgctgaaaat
    21481 caagtataat accataggaa caaactggaa ggtgaaaaag agcatggcac aactattttt
    21541 taagtatggc gcaatgaata gcggcaagac gatcgagatc ctaaaagtcg cacataatta
    21601 tgaagaacaa aataagccag ttgtcttaat gacaagcggt attgatacaa gagatggttt
    21661 aggcgttgtt tcaagccgaa tcggactgaa acgtaatgcg atgccgatat ttgaagaaac
    21721 agacgtattt gacgtaattc agaaaatgga aagcaaaccc tattgtgtgc tgatcgatga
    21781 agcgcaattc ttaaaaaaga aacatgtcat tgcttttacg agaattgtgg acgaattgga
    21841 tattccggtt atggcatttg gtttgaaaaa tgattttcga aacgagttat ttgaaggatc
    21901 aaaatattta ttgctttatg cagacaaaat cgaagaaatg aaaacaatct gttggttctg
    21961 ccacaaaaaa gcaatcatga atttacatta tattgatgga aaccctgttt atgaaggcga
    22021 tcaagtgcaa attggcggaa atgaagctta ttaccccgtt tgtcgaaaac attactttca
    22081 cccatcaatg attacagaag gagaataagc atgtacgatc aattacaagc aattgaagac
    22141 cgttatgaag agcttggaga acttttaagt gacccagaag tgatttctga tacaaagcgt
    22201 tttatgcagc tttcaaaaga agaagcaaac acgcgcgaaa cagtcgaagt ttatcgtcgt
    22261 tataaagaag tagtacaagg aatcaccgat acagaagaac tgcttggtga aaaactggat
    22321 gatgaaatgg ctgaactagc aaaagaagaa ttatctgaat taaaaaaaga aaaagaagtc
    22381 ttagaagaaa aaatcaagat cttgctttta ccaaaagatc caaatgacga taaaaatatc
    22441 atcatggaaa tccgtggagc agcaggcgga gacgaagcag cattattcgc tggcgatctt
    22501 ttcaatatgt accaaaagta tgctgaagca caaggctgga aaacagaagt gatggaagct
    22561 aacgttaccg gaatcggtgg atataaagaa gtgatcatga tgatcaccgg agacaacgta
    22621 tattcgaaat taaaatatga aagtggtgcg caccgcgtcc agcgtgttcc ttcaactgaa
    22681 tcgcaagggc gtatccatac ttccaccgca acagttgtcg tgatgccaga agctgaagaa
    22741 gtagagatcg atattgaaga caaagatatc cgtacagata tctatcatgc ttcaggtgct
    22801 ggcggacagc acgtcaataa aacagcatct gctgtacgtt tgactcactt accaacaggg
    22861 atcgttgttg ctatgcaaga tgaacgttct caaatcaaga accgtgaaaa agcaatgaaa
    22921 atcttgcgtg ctcgtgtgta cgataagatc cagcaagaag cacaaagcga gtatgatgcc
    22981 aatcgaaaat cagcagtcgg aactggggat cgttcagaac ggatccggac gtataatttc
    23041 ccgcaaaatc gtgtgactga tcatcgtatc ggattaacta tccaaaaatt agatcagatt
    23101 ttagcaggga aattagatga gatcgttgat gcattgatca tgtatgatca aacatcaaaa
    23161 ctggaagcta tgcaaaatgg gtaaaatgac ctatcgagaa gtcctgtcac gggcttcttc
    23221 ttttttagag gatcatggga aagaagcata tagtatccaa tttttatttt tggaacgaaa
    23281 acaatggaaa aaacttgatt ggctccttca tatgaacgaa gagatttcag aagaagaaca
    23341 gcggttgatc gaaaccgatc tgcgacttct gcttgcagat catccacctc agtatttgct
    23401 aggatatgca gatttttatg atcatcggtt aaaggtaact gaagcaacac tgatccctcg
    23461 accagaaaca gaggagttag tcgaatggtg tttagacgag acgcctggtg taccgttaga
    23521 agtgatagat attgggacag ggactggagc aattgctatt agtttgaaag cagcaagaaa
    23581 aaattggcat atatctgctg tggatctttc agaagaagca ttagaagtag cgaaagaaaa
    23641 tgctcagaaa gaaggaacga aaatcagttt ttatcatgga gatacattag gaccagtaat
    23701 ggatcagcaa tttgatgtga ttatttctaa tccgccgtac atcagtcgga atgaatggga
    23761 actgatggat gaaagtgtcc ggtcattcga acctaaaatg gccctttttg ctgagaacga
    23821 tggattagct atttatgaaa aaatagcagt tgaagcacca agcgtactaa aatcaaatgg
    23881 aaagattttt ttagaaatcg gatttcgcca aggtgaagca gtaaaaaaaa tatttcagca
    23941 agcatttcca gataaaaaag tggcgataaa aaaggatctt tttggcaatg aacgaatgat
    24001 ccaagtttat ggttgaagtt gtggatgaaa aattttttat ttgttttttt attataaaaa
    24061 aacacttatt tgacaaggat gtaaacgact ttttggttta tcaacaagtt tatccactat
    24121 tgacaaatag attatccaca aacagtggaa aacttgagga taaactcaga caaaataaga
    24181 atgtatgaaa aaggggttgt ggataaactg tggaaacgaa acgatatgat gaaacagaat
    24241 tgcaagaggc agccgaagca ctgaaagaag gagaacttgt tgcttttcct accgaaacag
    24301 tatacggatt gggcgccaac gctcttttac caaacgctgt caagaaagtg ttttctgtaa
    24361 aagggagacc gcaagataat ccattaatcg tccatgtcgc ctcttttgaa caagtaaaag
    24421 agtatgtgga caactttcat ccggcagcag agaagattgt gaagaacttc tggcctggac
    24481 cattaacttt gatatttaag atcaaaaaag acactttgcc ctcagttgtg acgggaggat
    24541 tatccacagc tgcatttcgt ataccggata acaaaaaaac actagaagta atcgaacttt
    24601 ccggtgttcc gcttgtcggt ccaagtgcaa atacgtcggg aaaaccaagt ccaacgactg
    24661 cagatcatgt atatcatgat ctacaaggaa aaattaccgg gattattgat gatggagcta
    24721 cgcgaattgg tgtagaatcc actgttcttg atttaagtga tccaacagcc atgcctatga
    24781 ttttacgacc aggtgcagta acgaaagaac aaatcgaagc agtcatcgaa tctcctgttg
    24841 ctattgatca gcatcttgta aaagaaaatg aaacaccaaa agcccctggg atgaaataca
    24901 aacactactc acccgataca cgtgttctga tggtcagaga tggcgattgg tcaactgcag
    24961 ttcaatgggc aaaaaataag aagatccgtg caggtgtcat agcaagcccc gaaattgcgg
    25021 atcaagtgcg gacagatact gcagctgttt atatgtataa cgacaactca gtagaagcgg
    25081 cggctaaagg attgtttgca ggtctaagag gattagatga accaacactt ggattagatt
    25141 tgatttttgt acaagtgtat ccagaaactg gattggggaa tgcttacatg aatcgcttga
    25201 aaaaagcagc aggacaaaat tattttgaaa aatgatcaat tggttattgt cgcattcttt
    25261 tagtatgata caatgctatc aagtaaatct tctatcagtg aacgaaaaac taagcgtaac
    25321 taatcgtaat agaagagata cgaatatttg gcaatttgtt tttgatggga aaaataaaat
    25381 tgtaggaaaa taaaaaggag ctgttgtttg tggtagatta caaaacgttt gaccctgatc
    25441 tttgggctgc gatcgccaaa gaagaagaaa gacaagaaca taatttggag ctgattgcct
    25501 ctgaaaactt tgtctccgaa gcagtgatgg ctgcacaagg aagtatcttg acaaataaat
    25561 acgcagaagg ttatccaggg catcgctatt atggtgggtg tgaatttgtc gatatcgttg
    25621 aaaatttagc gattgaccgt gcaaaagaat tattcggagc gaaatttgcg aacgtccagc
    25681 ctcactccgg ctctcaagcc aacacggctg cttatctcgc tttggtagaa ccaggggaca
    25741 cgatcttagg aatggatctt tccgcaggcg gacatctaac acatggatca ccagtcaact
    25801 tcagtggcaa aacctatcac tttgttgctt acggtgttga tccgacaaca gaagttattg
    25861 attataatgt ggtacgtatt ttagctagaa aacatcaacc aaaattgatc gtagcaggtg
    25921 ccagtgctta cggacgaacg atcgactttg cgaaattcag agaaatcgca gatgaagtag
    25981 gcgcaaaact gatggtagat atggcacata tcgcaggatt agtagcagca ggacttcatc
    26041 ctaatccagt tccttatgca gatatcacaa cgactaccac tcataaaaca ttgcgtggcc
    26101 ctcgaggcgg catgattttg acaaacgatg aagcattagc aaaaaaaata aatagtgcgg
    26161 tatttcctgg tattcaaggc ggccctctag agcatgtgat tgcgggtaaa gcagtagcat
    26221 tcaaagaagc attagatcct gcatttaaag aatatagtga acaaatcatc gccaatgcaa
    26281 aagcaatggt caaagttttc aatcaagcaa ttggaaccag agtcatttca ggggcgacag
    26341 ataatcatct gatgttgatc gatgttcgcg agttaggtat aaatggaaaa gaagcagaga
    26401 gcattttgga cagtgtgaat atcactgtaa ataaaaactc gattcctttt gaaacactaa
    26461 gtccattcaa aacaagcggt attcgaatcg gcacgccagc aatcactact cgcgggttca
    26521 aagaagaaga tgctgtaaaa gtggctgaac tagttgtcaa agcattacaa gcaaaagatg
    26581 ataacgctca actagacgaa gtcaaaacag gtgtacgcga attaactgaa aaatttccgt
    26641 tgcataaaaa ataaaaattt aaattccatt agtaaacaga aaagttcaat gttgggcttt
    26701 tctgtttatt ttttgggaaa acggattctt tgacaatatc ggtgatgctc tctagtatat
    26761 gaggtgtaat tttagtcttc acactaaatc tcagggagga aaagtcatga ctgatcgtat
    26821 ctcgttagaa aaggaagcag tagcattcag tgatgcaaat gcgcctcatc cgcgtatcta
    26881 tgaactagca ccggaagaag gaagagtttt actagaagaa gtacaagaat cacctgtgaa
    26941 caaatattcc gtcgatattg aagacttgat ggtggatact ggcgaatggg gaaaactcaa
    27001 tattcgtctc gtacgaccag aagggaatat ggataaatta ccagttattt tttatattca
    27061 tggagcaggc tgggtttttg gcagtgctca aacccatgat aaattgatca gagaacttgc
    27121 ggttcgtacg aattccgtcg ttgtctttcc agaatatagt cgatcaccag aagcaaaata
    27181 tccaacagca attgaacaaa attatgctgt gttgcaacag ctgaataaat tagccaagtc
    27241 aaaaaatctg gatctcgaga atctaatcgt tgcaggagat tcagtaggtg gtaacatggc
    27301 tacagtcatg acgatcttaa caaaagaacg aaagggcctc ccaatatcca agcaattgct
    27361 gtattatcca gtcacaaacg ctgattttga tacagattcc tatcatgaat tcggagaaaa
    27421 ctattttttg acaagagaag gaatgatgtg gttttgggac caatatacga cgaatgaaga
    27481 agagcgtgct gaaatcacag cttcgccttt gagagcaaat ataaatgaac taaatgatct
    27541 tccttcagca atgattttaa cgggagaagc ggatgtatta agagatgaag gtgaggctta
    27601 tgcgcgcaaa cttcgtgatg caggggttcc tgttacacaa gtacgcttcc aaggaatcat
    27661 ccatgatttt gtcatggtaa atgctatgga tcagacaaag gcaacgcgtg cagcaatggc
    27721 attatctact tcttggatca agaaaaaaga ataagctttg cttgtccgca ggaaaacttc
    27781 ttttcaaaaa cggattttct taggaaaaat ggcgagtttg actagtcaat cacttgattt
    27841 tgttttacaa ttaaaaagaa acgacagaac aaaggagagc ttaaaaaatg ggcaaatttc
    27901 aagtgatcga tcatccattg attcaacaca aactaactat tattcgggac aaaaactgcg
    27961 gaacaaaggt ttttcgtgaa gtagtcgatg aaatcgctat gttgatggca tatgaagtat
    28021 cacgggatat gccattggaa gacatcgtga tcgaaacacc gatcactgaa acaacacaaa
    28081 aaacgttgtc tggtaaaaaa gtagcgatta ttccaatctt gcgcgcaggt attggaatgg
    28141 ttgacggtat cttggaattg attccagctg ctaaagtggg acatatcggt ctttatcgag
    28201 atgaagaaac attggaacca catgaatatt tcgtcaaact tccagaagac attgatgcac
    28261 gtcagttgtt tgtagtagat ccaatgttgg caacaggcgg atcagcaatc atggcaatcg
    28321 atgcattgaa agaacgtggc gcgtcgaaca ttaaatttgt ttgtttggta gcagcacctg
    28381 agggagtaaa agctttgcaa gaagcgcatc ctgatattga catctataca gcttctctag
    28441 atgaaaaatt gaatgaacat ggttatatcg ttcctggact aggggatgcg ggtgaccgtt
    28501 tgtttggtac aaaataagta aatacgaaaa agggtgtgac acaacttttg tcacatcctt
    28561 ttttggtcat ttttaacgtt tgatgcagaa cagctttttc acagctttcg tttgcatata
    28621 aaaaaaccat cccaaaaatg cagagatggt caaattcaaa tcttttttag ttctttgtag
    28681 ttaacgatta ctacaccctt actttgcgtc atcaagttcg cattttctgc agtttcatca
    28741 atctcaacaa cagcggaatt tttcaattgc tttgaaatcg ttccagtttg cgacttgccg
    28801 tgaattgtga acattacgtg agtattgatc ttgaattttt caccaatttc cgatggtacg
    28861 atagttgtat caaattttcc aaaagttgcc attttcataa aacctgcctt tcacatcaat
    28921 tataccacgt tttatgaaaa atatcatgaa attaaactaa ttttccatcg gactctttac
    28981 aatcagccaa atagagtata taatttcttt tggataaggc cagcttagct cagttggtag
    29041 agcaacgcac tcgtaacgcg taggtcgtag gttcgatccc tatagctggc attaaaagag
    29101 aagacagata tatcaaggat ttttaatcca ttgatacatc tgtctttttg tttaaaggaa
    29161 ataaagtatg ctttcttagc aatagagcat gtattatttc tataaaaatt taggagaata
    29221 aaacgactag caatatagct gtgaaatcaa atatcacatg aactgcccaa cttgtaatga
    29281 tcgagttgct tttaatggca gcttggttga ataacaatct tgccacccct tgtatcaaaa
    29341 ggatgtgtgc tacagtgtgt aaaatatttc cattatcata agtactgtaa tggataaatc
    29401 caaagatgat tgctgacaag aaactagcga caggaatgct gaacttacta gaaaaaatag
    29461 cgagaaaata cactgagaac aattcttctc caagtatcat gataggcaaa ataataaata
    29521 ataacgggct gaaagcctca tttattggat tgcctgttaa accccatttc aaaatttgtt
    29581 gcaaaaatac cgagactgcg gcactgatta ctacattgat aaaaaaatat ttggctatat
    29641 ttttgacagg agcgacaggt ttcgagaaca tttttttcgt atctttttta ccaaaaacaa
    29701 gtgtaaatgc tataaatcca gtgatagtta aagaaaaaat gctaataatg gatggcgcaa
    29761 atagcaaccc taacaaaccg agtacaacga caatagtgcc caatatccct tttttaagtt
    29821 ctattttttt tcctaaaatg ttattcatga taataactcc tactattcag tttttgaaga
    29881 t
//
LOCUS       DAXWOO010000013        29208 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.013, whole genome shotgun sequence.
ACCESSION   DAXWOO010000013 DAXWOO010000000
VERSION     DAXWOO010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29208
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.013"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..132
                     /locus_tag="ACGE70_000487"
     CDS             <1..132
                     /locus_tag="ACGE70_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HFF8692152.1"
                     /translation="NITSISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            308..1052
                     /locus_tag="ACGE70_000488"
                     /pseudo
     CDS             308..1052
                     /locus_tag="ACGE70_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            1045..2625
                     /locus_tag="ACGE70_000489"
     CDS             1045..2625
                     /locus_tag="ACGE70_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFF8692153.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(2876..3355)
                     /locus_tag="ACGE70_000490"
     CDS             complement(2876..3355)
                     /locus_tag="ACGE70_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFF8692154.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(3371..4123)
                     /locus_tag="ACGE70_000491"
     CDS             complement(3371..4123)
                     /locus_tag="ACGE70_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFF8692155.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            4240..4310
                     /locus_tag="ACGE70_000492"
     tRNA            4240..4310
                     /locus_tag="ACGE70_000492"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4272..4274,aa:Gly,seq:ccc)
     gene            4671..5396
                     /locus_tag="ACGE70_000493"
     CDS             4671..5396
                     /locus_tag="ACGE70_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFF8692156.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            5651..5917
                     /locus_tag="ACGE70_000494"
     CDS             5651..5917
                     /locus_tag="ACGE70_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFF8692157.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            5914..6345
                     /gene="ruvX"
                     /locus_tag="ACGE70_000495"
     CDS             5914..6345
                     /gene="ruvX"
                     /locus_tag="ACGE70_000495"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFF8692158.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            6370..6675
                     /locus_tag="ACGE70_000496"
     CDS             6370..6675
                     /locus_tag="ACGE70_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFF8692159.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(6756..7901)
                     /locus_tag="ACGE70_000497"
     CDS             complement(6756..7901)
                     /locus_tag="ACGE70_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFF8692160.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(7962..8609)
                     /locus_tag="ACGE70_000498"
     CDS             complement(7962..8609)
                     /locus_tag="ACGE70_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFF8692161.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            8803..9096
                     /locus_tag="ACGE70_000499"
     CDS             8803..9096
                     /locus_tag="ACGE70_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692162.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            9140..9451
                     /locus_tag="ACGE70_000500"
     CDS             9140..9451
                     /locus_tag="ACGE70_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692163.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            9448..10072
                     /locus_tag="ACGE70_000501"
                     /pseudo
     CDS             9448..10072
                     /locus_tag="ACGE70_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            10213..10371
                     /locus_tag="ACGE70_000502"
     CDS             10213..10371
                     /locus_tag="ACGE70_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692164.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            10372..10734
                     /locus_tag="ACGE70_000503"
     CDS             10372..10734
                     /locus_tag="ACGE70_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692165.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            10771..11619
                     /locus_tag="ACGE70_000504"
     CDS             10771..11619
                     /locus_tag="ACGE70_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HFF8692166.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            11609..13084
                     /locus_tag="ACGE70_000505"
     CDS             11609..13084
                     /locus_tag="ACGE70_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HFF8692167.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            13350..13757
                     /locus_tag="ACGE70_000506"
     CDS             13350..13757
                     /locus_tag="ACGE70_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HFF8692168.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            13760..13975
                     /locus_tag="ACGE70_000507"
     CDS             13760..13975
                     /locus_tag="ACGE70_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692169.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            13979..14359
                     /locus_tag="ACGE70_000508"
     CDS             13979..14359
                     /locus_tag="ACGE70_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFF8692170.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            14493..14648
                     /locus_tag="ACGE70_000509"
     CDS             14493..14648
                     /locus_tag="ACGE70_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HFF8692171.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            14717..15190
                     /locus_tag="ACGE70_000510"
     CDS             14717..15190
                     /locus_tag="ACGE70_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFF8692172.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            15187..16881
                     /locus_tag="ACGE70_000511"
     CDS             15187..16881
                     /locus_tag="ACGE70_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFF8692173.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            16847..17032
                     /locus_tag="ACGE70_000512"
     CDS             16847..17032
                     /locus_tag="ACGE70_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692174.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            17036..18211
                     /locus_tag="ACGE70_000513"
     CDS             17036..18211
                     /locus_tag="ACGE70_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFF8692175.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            18204..19727
                     /locus_tag="ACGE70_000514"
     CDS             18204..19727
                     /locus_tag="ACGE70_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFF8692176.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            19783..20067
                     /locus_tag="ACGE70_000515"
     CDS             19783..20067
                     /locus_tag="ACGE70_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFF8692177.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            20054..20389
                     /locus_tag="ACGE70_000516"
     CDS             20054..20389
                     /locus_tag="ACGE70_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFF8692178.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            20684..21310
                     /locus_tag="ACGE70_000517"
     CDS             20684..21310
                     /locus_tag="ACGE70_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692179.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            22052..22888
                     /locus_tag="ACGE70_000518"
     CDS             22052..22888
                     /locus_tag="ACGE70_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFF8692180.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            22953..23387
                     /locus_tag="ACGE70_000519"
     CDS             22953..23387
                     /locus_tag="ACGE70_000519"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8692181.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(23455..24777)
                     /locus_tag="ACGE70_000520"
     CDS             complement(23455..24777)
                     /locus_tag="ACGE70_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFF8692182.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(24931..26571)
                     /locus_tag="ACGE70_000521"
     CDS             complement(24931..26571)
                     /locus_tag="ACGE70_000521"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFF8692183.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            26901..27371
                     /locus_tag="ACGE70_000522"
     CDS             26901..27371
                     /locus_tag="ACGE70_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFF8692184.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            28000..29103
                     /locus_tag="ACGE70_000523"
     CDS             28000..29103
                     /locus_tag="ACGE70_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFF8692185.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
BASE COUNT         9802 a         4891 c         5942 g         8573 t
ORIGIN      
        1 aacattacat ctattagcag agtaaacgaa atagtaacat ttgcaaatgg agaacaacta
       61 gagatgggaa agagaagtat tgataaacta aagaatttta tgcaagaaaa gatagaaaat
      121 gaatcatttt aaagaaaaca aagactcata tcttacctgt tagaagagtg ttttcctgta
      181 gaagagaaca acccaagtag cagcctagtc gtttctatcc ttttttgtta tgcactcttt
      241 ttgacattta ctccaccaat aacgaagggt acaataaaaa gtaataacaa acaaaaggag
      301 tacagaaatg attgaattca tcaatgtaag taaaagttac gggaaaaaac aagcattaaa
      361 agacttgtct ctctcgataa gacaagggga aatttttgga tttttaggac ataatggtgc
      421 cggaaaatca actacgatta aaagtttagt tagtattatt gaaccatcat cgggaacgat
      481 tttagtagat ggaatgaaat tgacggaaaa tcgattgagt atcaaacaaa aaatcggtta
      541 tgtgcctgat tccccagata tttttttaca attgacggct ggagaatatt gggacttgat
      601 ttcagctgcc tatgaattag acacacaaaa aaaaagaaaa gcgtttagca gaattgacag
      661 cgctttttga tatgtatagt catcaaaatg aaacaatcgc aagtttttca catggaatgc
      721 gtcaaaaaac gattttgatc ggcacgttgc tgccagatcc agatatttgg gtattggatg
      781 aacctttgca aggacttgat cctcaagcag catttgattt gaaagaaatg atgaaggctc
      841 atgccgcaaa aggaaaaaca gtcatttttt ctacacatgt attggatacc gcacaacagt
      901 tatgcgataa actggctatt ttgaagaaag gtgaattgat ttatcagggg gcagttagtg
      961 aattattgaa tggctcacct gatgaaacgc tagaaaagat ttatttgaaa atggctggaa
     1021 ggcaagcgac aggagatgga ccgtatgtat aagcgacaat tattagaatt aattcgagtt
     1081 aatcttttat atgttaatcc acagctcacg aataaagcaa gagagaaagg aaaaaaggga
     1141 aagcgattaa ctatctatct tttgtctcaa aatctttttt cagggctgat ttttttactg
     1201 atttacggat ttgctatgtt tgctatgaac tttgttaaat tgccaggttt ctttacttat
     1261 tacgtggcac tttttggtat gttaggttta actcaaagta tatcagtcat ttataatatc
     1321 ttttttgaag gaaatgacct gcaagtattt ttgcctctgc cttttggaca agaacaggtt
     1381 ttttcagcaa agattttggt agttgctttg accatcattc cttttgtttt tccgcttcta
     1441 gtcatgttta ttttaaccgg atggcgagct gatctaggga ttttgcttac tatcattatc
     1501 tcaatccttt tgttttctct atttttgatt attctttttt gtctggctag tttgattgtt
     1561 tttggtttgg ctcgaacagc agtgtttaaa aaacatagaa aaacagtttc tagtatcttg
     1621 cttggcagct cgatgctgat cgcatttgct ggaatcatgt ggatgaatca tcaaacgggg
     1681 actattgaaa atgagttaac agatcggcat ccgatttcga tattattgcc attctatgat
     1741 gctgccagtc agccactttc tcaacaaggt ttattgggtt ggggaatact agctatcacg
     1801 gctctgggat ttttccttgc tgtaaaacag ctgatagtac caaagcttac agagcaactg
     1861 actgattcaa acagtgagtt caaaataaaa agaacccata aaaagaacca aaatgtgcat
     1921 cagcttttat ttagttacaa tgctcaatta gtaaaagaac ctaatttgat tatgcaagtt
     1981 ctttcttctt ccttgttaac tccaatcatt tttattcttg cttttgccct aggaggcgaa
     2041 ataaaattaa ctgatttaga taataagtta atcggtgttg ttttcctggt agggatcgca
     2101 ttggctttct taacagttaa tcagacttct tttatcagca atcttatttc tttggatcaa
     2161 gaaaattttc tgtttatccg ttctttgcca atctctatga accaatattt aaaagaaaag
     2221 ttcagattcg gatggatatt gcagagtttt ttaactggtg ggatcgcatt attaagcgga
     2281 ctgtcttttc agctgccttt gttttttatc cctagcttgc tatttggttc tttattcggc
     2341 tgctacttat tgtcactacg ttatttcgct agagattatc ggttgctgct gctgaactgg
     2401 accaatatta accaattatt taacagaggt tcaggaagtt tgggtttagt cgcaactatg
     2461 atgggttcat tgatcatcag tattattctt atcgtcttat acggaatgtt ggctttgttt
     2521 tatccatttt ggttagtgaa tattcctgtg atcggtttag ttctaatact ttccgctcta
     2581 tggatcgttt attatcaacg aacgttttgg aaaaaatttg aataaagaag aggggccgtg
     2641 acaaaaatca tgtcgcagcc ccttttttac agatagaggg tgttcaaaag tcagtttctc
     2701 agtattaagt caattttatg taaaaatgtg agaagctatt tttacaaaac tttttttaat
     2761 actcataatt acttcttatt gctcctgcgg ttacttgtcg caatgtttgt gagatatttt
     2821 gtcatcgctc aaggctgaac gtctctttca caaccttttc tttgttacac ttcgtttatt
     2881 taaaaagccg tttttttcgt ttcatcatga ttgcgtcacg ctttactttg aaattacgga
     2941 tgttatggtt aaagacttct gataatacaa gacctaaagc gattgctcca gcaatcataa
     3001 ttgcttggac cgcataatga gccgcttctc gataggattc agtaacgaga tttcgaaccg
     3061 cttgataagc taaaccacca gggactagag gaaccatccc gggaatattg aaaatcgtta
     3121 cgggcatttt caaacgttta gagaaaagat cgctgatgaa tgctactcct aaagaaccaa
     3181 gcaatgaacc aaaagcagca gatccgccta tttgaacaca gatccagtag atcatccagc
     3241 caaaagcacc agaaagacca caacaaatca atgaacgcct aggtacattt gtaataacgg
     3301 caaatgccgc agtcgctaaa aaactaaaag aaaactgaat aaatatcgtc cccatacttg
     3361 tgttcctttc ctaataaaaa agctggaaaa taaaagcaat cgcaaaaccg atcatcgtag
     3421 cagtcatcat cgcttctgtt ccgcgagaaa ctcctgagac ataatgtcca gctagcaaat
     3481 cacggatagc gttcgtaatt tgaactccag gaactaaagg catcacacat ccgataatga
     3541 ttagatcctg attgcttcct aagccaaaac gtacgctgta gatagcggct atgccaatca
     3601 ataaagaaga taaaaattcc gataaaaatt tgattctaaa aaatttcaaa ctatatagat
     3661 aagctgcgta tcccaatcct ccaatgacta aagtcacagg taaatcggtc atctttccgc
     3721 caaataacac cataattgtt ccactgatca ctgccgcgct aacgatttgc aaccagacag
     3781 gaaagaaaga acggtctcgg tcgattgctt gcagttcgca gtaaagttct tctaatgtta
     3841 gctctgaagc aacatattta cgtgaaagat catttacttt tactactttt tctaaattaa
     3901 tcgaacggtt ggtaatttgt tccattcgga tggtcgaggt acgatcaaga ccaataaaga
     3961 gaccggtttg agtgacatag ctgaccaaac ggaaatttcc agaagctaaa gcaattcgac
     4021 tcatcgtatc ttctacccga tacatctccg cattgctttc catcatgatt ttcccagcga
     4081 gtaaacaagt gtccagcaac agatctaatt cttttcccgt catagcatct tctccaataa
     4141 ttgattgatt agttttattt tactacaaaa cataccagaa gcgtaaagaa taaaaaaact
     4201 tgaggaagga ataaaagagg cgtatactgt ttttgtattg cgggtgtagt ttagtggtaa
     4261 aatcccagct tcccaagctg atgtcgcggg ttcgattccc gtcacccgct taaaataggt
     4321 tgtgaaaaag ctgctctgca acaagtaata agaacagcca agcaaaaaca acttcttatg
     4381 tttttaactt ggatggactt attaccgcag atgcaagctt ttgcgcaccg tttaccaagc
     4441 ggttgtgaaa tcagtgtatt gtcttgagta tgaagaaaat ttttgaataa taataaatac
     4501 cttacagata gagtggtgac tgattagaga cgaggaatcc gtctcttttt tgtattttta
     4561 agccatctta aaacaggttt tattttttgc tgtttctatt tctttctagt atttatttat
     4621 aggggaacat atgttaagat ttagggagct gttagtaaag gagtaaagaa atgagcaaaa
     4681 aaacaaccaa ggcgatcgtg gggatcgtcg ctgctgtgat tgcaggggca ttcggtgttt
     4741 ccaccacaca aaaagattca gatatctttc agcagattac aactattttt gattccacac
     4801 cacaagcttc tcaagtagcg gatgtgccag aatatgatgg aaaacatcaa gaaatagaaa
     4861 tcaatgggaa tcagccggaa tttacaaaag aggatcttag cctttccaaa ggaacatggg
     4921 aaagctattc aaatattgat aaattgaaca gagtaggaca agccaatgct atgcttggaa
     4981 aagatttgtt ccctactgaa aagagagagc ctttgtatat tgacccgaca ggttggaaac
     5041 aaaagaaatt aagtgacggg caatggttat ataatcgcag ccatttaatt ggtttccagc
     5101 tgactggtca aaataacaac attaaaaact tgatgacagg gacacgttcg ttgaatgctc
     5161 catacatgct tgctcacgaa aatgatattg cggcatatat caaagaaaca aatcatcatg
     5221 tacgctaccg tgtcactcct cattttgagg ggaatgaact ggtagccagg ggagtgcaat
     5281 tggaagcaga aagcattgaa gacaaaaaaa ttgaattcaa tgtctttatc tacaatgttc
     5341 aagatggcta cacgatcaat tatgagacag gacaagcaac aaaaacaaaa aattagtagc
     5401 agctattcga attttttatg aatattatga aaaaattgtg taaaaacata cattcaaaga
     5461 aatatagcgt atactttatg aaaagagtac ttttgataaa gctacagaaa aaaggaaaaa
     5521 aacatacaaa tagtgtttct taatttattc ggaagtttgg aatatttgtt tgtagttttc
     5581 cctttcttcg agtaaaataa ggttgtagaa ggtaagctgg aattaccttg cgggaaagag
     5641 gtgtagaatc atgggtttta cagatgaaac cgtacgtttt ggttttgacg atagtcgcaa
     5701 aaaagaagtg agcgaaacat tagcgattgt ttatcgggct ttggaagaaa aaggctacaa
     5761 tccaatcaat cagattgtcg gctacttgct ttctggagac ccggcctaca ttcctcgtta
     5821 tcgtgatgct cgaaatttga ttcgccgtca cgaacgcgat gagatcatgg aagtaattgt
     5881 caaggactac ctctcaaatc atggagtgaa tttatgagag taatgggctt ggatgtcggt
     5941 tctaagacag taggtgttgc tatcagtgac ccctttggct ggacagctca aggagtagag
     6001 atcattcgga tcaatgaagc agaagaagag tttggctttg atcggttgaa ggaattagta
     6061 gcacaatatg aagtagaccg gtttgtagtt ggattaccta aaaatatgaa caattccatc
     6121 ggaccaaggg cagaagcttc catgtcatac ggagaaaaga tcaaagaact attctccttg
     6181 ccagttgatt atcaagatga acgattaacc actgttcagg cagaacgtat gctagtagag
     6241 caggcaaata cttcaagagc caaacgaaaa aaagtcatcg ataaattagc agctgtgatg
     6301 atcttacaaa attatttaga cggctcaggt aagcaattat tttaataata aaagaaaaga
     6361 ggtaatctta tgacagaaca taaccattcc cacgaccatg aaggacacga acacatcaca
     6421 ttggtggatg accaaggaaa tgaaacatta tatgaaatcc tactaacagt tgacggacaa
     6481 gaagaatttg gacgtaacta tgttctttta tatcctgctg gtgtttcaga agatgaagat
     6541 gtagaattac aagcttatgc atacatcgaa aacgaagatg gaacagaagg cgagttagaa
     6601 caaatcgaaa cagacgcaga atgggacatg attgaagaag tattcaatac attcatggcg
     6661 gaagaagaag aatagactat aaagctagga gaaaccttga tatgataggg tttctccttt
     6721 ttttattttt tactgtatgc catattgtat gccatctagt caatatttaa gtgttgtact
     6781 aatttttcaa cagtcttttc tttcgtttct tttgccaagt gaccgtaaca atcgatcgtc
     6841 tgactaattt ttgtatgccg taatctagtt tgaacttcta ccatagtagc tccagcgtcc
     6901 aagagcattg aagcatgagt gtgtcttgct acatgcacac cgagaaatgg aaccatagct
     6961 ttttcacaaa tagtaatcaa tcttgcataa atagcgtggt gtgtgattag ttctttatag
     7021 ataccacaaa aaatcatagt cggatcagtt acaccaagtg tcagcatata tttccgctga
     7081 ttaatctgcc atttttttaa tatagaaatt gttctttcat ctagtggaag aaccccagca
     7141 ctacaatcag ttttaggatc actgatttta tagccatttt tggattggct tagtgtttta
     7201 ttgatggtga ccgtattttc ttgaaaatca atgtctgacc aattcaatgc aagggcttca
     7261 ctgatcctaa ccccgctata aaagagaaag cgtagtaacg ttttatcaaa gtcgtttcga
     7321 taagtttcag gtttgctgtt taggtaatca aacaaggtga tcagttgttc tttactgtaa
     7381 actttgacct tttctttatg cttagtggtg gttcttttag gcattaatac gttgtctagt
     7441 ggattagtct caataatccc aagattgata gcgtagctaa aaatacgctt agaagttgaa
     7501 tgaagtacct tgtattggtc agtagtttca gcccagctat ttacaatttg ttgggctttt
     7561 tttaccgtga cttcattcac acgaagattt ccatattttg gaagaatcca ctttctaaac
     7621 ttgatttcag ttttcatgaa ggtggcttct cggacagtgg ttttatatgt ttctagccac
     7681 aagtcataaa cttctttaaa cgtttcgttg ttcgcttttt taataccgtt tttatcgtag
     7741 tccacttgca agcgtgacaa ggcaagctgg gcttcttttt ttgttttgta acctcgtcta
     7801 cgaaccttat caggtttgcc agtcattttg ttcattccaa gataggcttc atagagccaa
     7861 aatttacctt ttttagtttc atattgcttg aatgttgcca taatagtttt ccctccatag
     7921 tgtagcaagc cgtgggggcg gttggaggag gcagtttatt attattttga atagtgttta
     7981 tttagctcag taacaagagc tgtgaactct tttagaagat cattttcata aaattcttga
     8041 tactcttttt tctcatctgt atttttttct gaatcattaa ttaatgagtc atatcgtaaa
     8101 agcgtattta gaagagctgc gagataagca attggggtac tcccgtcagg aaattcaatt
     8161 cgagatcctg ttttaagata ttttattgcg tttgttaaat aaatcttctg tgagacagtc
     8221 aggttatctt cttcgatgtt attgatagca ttagaaagat tagaatcaat tatagagtta
     8281 tttttttcaa tagttttttt caattgcttt tcaagcacat ttcgcttaat ttcgggcatc
     8341 atatgcatat ctcttaatgt ataagatccg tttaatagat aggtagtgga tacatctaat
     8401 acctttgaaa gttgtttaag cctggtgtta ttgggaagat tatagccatt ttcccaatta
     8461 ctaacggcac ctttactggc cggtggggat aatttacctc caagttgttc cattgagagt
     8521 cccttttcaa ttctagccag tctaattctt tgtcctgtct ccactgcttc ggggtatttt
     8581 tttctagcca cattttcacc accattcata tattttctct actatatcac gatttaaata
     8641 tgtgtgaaac ataaaagttc aaaaaagtat gttaaaattc ttgcaaatga aaaggtatga
     8701 tggtatattg agtttataag aggtatagaa atgtattcaa gagcctcttg cattttttta
     8761 aaatctttgt atagaaatgt atgctaacga ggggggataa atatggaatt aaaaattgac
     8821 aaactcacgc ttgatgatcc agcttttaaa attattgacg atcgagttgt tcaacaagta
     8881 gagcaaactc aaggaacgtt actttcatta ttagaaaaaa agtaccagtt tccaaaatat
     8941 atgaacaagg cacaagctgc taagtatatg aacgtttcat acaatactat gatgcagaaa
     9001 tatgttccta acggtttaaa gttgattata gttgatggag tcgtaagaat ttcacaagat
     9061 gaatgtgatc gctttatgga agaataccaa aaataaacgc aagccgtggg ggcaaaggga
     9121 ttaataggag gaaataataa tggaatcgac acaaaataaa gcaatcgaaa aagtattatc
     9181 aacaattctt acagaagaca cagcggcaga attagcaaac ttggaaggca aagcgctaga
     9241 agaaacgttt gaatgtttgt acgaacaaat ggactatcaa aaattgctac cacaagaacc
     9301 aacggtaagc ggagtattgc gtggcttgaa cgatttagta caagcagagt ttaaagaacg
     9361 actttctatt gaagagtatc aagaaatttt gtatcagcaa gttgagttgt tagcgagctt
     9421 gctagggctt gaactggagg acgttgaatg aaaactgaaa tttggaacgg acacattata
     9481 agatttgtcg atattaatga tgaatggtgg gcagtggcga aagatgttgc ggaagcgtta
     9541 ggtcttaaac aggttactag agccattcac tctttgccta aagatggggt tactactagt
     9601 aaggtcatcg attcattggg cagaacacaa gatgtaaaca tcatcaacga aaaaaaatat
     9661 ttaccgcatg gcattcaaaa gccgtaaaaa agaagccgag gcatttcaag actggatttt
     9721 cgacatcatc aaagaactac gccagtccac aggacttgaa ggctttcaag tattccgcat
     9781 gctagacaaa gaacaccaaa aagaagcaat gactaaatta agccacgcta ttactgaacc
     9841 aaaaccagta gactacatta aagcgaatgt gatagccaat aaagctgtat caactattta
     9901 tggtcattcc aaaatggtaa aaaagaaaga tatgactcct gaaatgttgg ttgatcgtga
     9961 acctatttta gatgagacag tagaattaat gactgttaaa gaaaaatatg ggttgcagtt
    10021 ttcagtgagt gagaaaattt ataatcgttc tgctgaattg cagacaactt aggagcatta
    10081 acatgatcga gaacaaagaa aaagccttat ccataacagt cttggcggac aggataaagg
    10141 caacttaatt gggataaaac ccgatattct ttgttccgat tataccatat taaaaaatcg
    10201 gagggaacca ttatgaaacg aaacttaatt ataggaagca ttttaatcgg cggaattttc
    10261 ggcttattac cattaattgc aaaaatagct atctttgcag gattagcgtt atatctcttc
    10321 actgcttatg acgagtacga ctatcaatta agaactggag ggcataagta gatgtacaaa
    10381 acaaaactat taaaccaact cgatagcctt gaattagagg aaattaatca agggatcgct
    10441 gaactagaaa ataatatcgg gaaaacctac tttggaaatt cattcaatga aaaactaaca
    10501 gttttgtatg tactcaaaaa acatgcagaa cacaaaataa tttgtcgaga aatcaacgag
    10561 ctaaaaaatc agattttaac cgcatggtta aacattactg atatgcaaga agcaagggtg
    10621 aaaacattta atacatgggt aaagtatcag aatcaattaa aaggtgctga atttgttcgg
    10681 gatgggttga aatacgaact tgagcagttg aagctcatgg aggtgtctga atgatttata
    10741 ccagtatttt gaaaaaggag gagaggttta ttggcaaata attatcaagc agcaacggaa
    10801 ttacaaaagt cgggatggtc aatttatccc ttatctcctg gaactaaaat tccaataaaa
    10861 ggctctagtg gttggaatga tgcaactaac gattctaatc aaattaatga atggtggacg
    10921 gaagatgcaa ctagaaatat tgggttaatg cttgaagatc atcaaatggt ggtagttgac
    10981 ctagatcaac attctgaaac acgaaacggc gttgagaatt ataaaaaact tgcttcaatg
    11041 tatgatccat ttccttctac ttatacggaa atcacaccag gtaaaggaat ccatttcttc
    11101 tttaaaattc ctgatggtgt aattatccaa caagaaacag gtgcatttgc tgatatcttc
    11161 gggcgtgatg ataaaggtaa aagtttaagc gggattgata ttatcactaa aggagttcca
    11221 atagcgccaa ctatgattat ttcaagaagt gttgggagag catatgaacc tattgaaaac
    11281 gtatctctaa ataatattgc accagctccc gattggctag tgaacctact gctaaaaaaa
    11341 ccgcaagaaa taaagactca ataccgaccc gtagcaaaaa cagggacagc aaaaaaatta
    11401 gatatgattg ttcaaggcgc tggcgaaggt agtagaaacg atcatttaac taagttatgc
    11461 ggttggttac tatggcacgg tgtagataat caaactttag cagaattgat ttatacagca
    11521 aatgcgtaca atgaaccacc gctaaaagat aaagaagtga atcaaattat tcgatcgatg
    11581 attaaaaaag atttgagggg gaaagctcat ggctcataag cataaattag atagtatttt
    11641 agactttccc gaagccagcg agcgtgaaga caatatcatt gaattgaaaa cctggatgtc
    11701 acgattaaga tgcaataaag acgatcagat taaaagtaac agtgttgtga atgcagaatt
    11761 gattttaacc aatgatagca atttagctgg aacaattgct tataacgaat ttagcggata
    11821 cattcaccta ttaaaagatt ctccttggat caatcgtacc gctggagaat gggaagacag
    11881 ttttgaggat gccttaacgg catatattga agaaaactac aatgtagttt ttgatgacaa
    11941 caaaatacac aaagcagtag taaatgtggc tagaaagaat gttttcaacc cagtaaaaga
    12001 acgtatcgaa aaagttaaat gggatcaaca gcctagactg gaaacactgt ttattgacct
    12061 tttaggggta gacgacaacc tttacacacg agaagtaaca aaacgttgga tagtcggatc
    12121 agtcgcacgt atctataaac caggaataaa gtttgagatc gttccagttt tggatggacc
    12181 gcaagggatt ggtaagagta ccgttccagc attactttac acggatgatt ttttcaccga
    12241 ttcattagat tcgctaggtg aaaagaaaga tgattatatg cagcttcaag gcaatgtaat
    12301 tattgaactt ggagaattat cttcaatgaa caaaactaaa attgaacaga taaaaaactt
    12361 catcagtgcc aagatcgata aaattagacc tccttatggt cggaatgtca tagcttgggc
    12421 aaggcaatgt gtatttattg gaacgtcgaa tgatgggcag tatttgaaag atgatacagg
    12481 aaaccgccgt ttttatcctt tgccttgtaa gaataagcca aagatgaatc cgtttaaaac
    12541 aaaggatgaa tattttcttc aagtgttagc tgaagcaaag gtgctttttg ataaaggaca
    12601 acgaatctat tttagtcccg atgaagataa agaagtctta gaaattgcaa aagactatca
    12661 agaggatgcc aagatggaga atccaattaa agaagcaatc actaaatatt tggaaatgga
    12721 aatcccgtat aaatgggaag aagctccagc gtgggcgaga cgttcttatt atcagaacta
    12781 tcctgacagt aaatgtaatg agaaaaattt acagcatttc tataatggag ctactatgca
    12841 acaagagttt tatttagttg atagcgtact caccgctgat attttggaag ctgtttttga
    12901 taaacaagca aaggatttat tgaatggtcg atcagatgca gaaactaaaa agattgcctt
    12961 gattattagc agtattcctg gttgggaacg gaagcaactg ccaaaaagaa ataaacgacg
    13021 aggattttac aataagtcaa atgcctatga aaataaaaat cgagctggaa agtacaaaaa
    13081 gtaacgggta gtaacgggtc agtaacacct agcccgttac tagtttaccc ttactgcccc
    13141 aagggataca gcaatttggt aacggggtaa caccttattt ccttaaaact tttgaaatag
    13201 ttagctaata tgctggggaa taatgttact ggattttacc cgttacctgt taccaaaacg
    13261 ctagaaccct tgtccccgtt gggattgagt ggtaacgggt acctgttacg taggtgttac
    13321 cacgtgttac gaatcaagga ggaaagtaaa tgctcaatat tatttcaaca aacaaagcac
    13381 cgaactttca atatacagat gaaatggatc ggtttctaat gaacacgctg gcatttagtg
    13441 tgggattggt aacagaagat tattccacat ttgatccaga agtattaaag ataatggaag
    13501 aagaacctga ttggctgcag gagtcagtag cgtggtgtca atcgctagta gtgggatcat
    13561 tggtagatag tggcaactat gatgatacag gtgagctaat ggatgaattt aattgtctgc
    13621 ttaacctata tgatcgagcg agacaacgag agcttacatc gaatgaggat aatttgtttt
    13681 taaacatcca tgataagttc ttggctttgc tactaacaga tgatgaattg ataactaatt
    13741 tattggaggt agaataatta tgcatatgga aagtattaga tacttcgatg gtgagaaaca
    13801 cattgagtat tcaaacactc aagaagatgt agactttatt agttttgagg aagataaaat
    13861 cactaaagtt aattttaaag acggaacgtg cttgaagata gtttcgcctt atattgaata
    13921 caaatcaaaa tgggaacaag atgattctga cgaacgagat cgctttggtt ggtaatctat
    13981 ggcagttaaa aaacaatgta atcatgctgg atgtaaaaca ttgattgatt ataggcagaa
    14041 gtattgtggg aagcataaag caaagcaaac ggcaattaag cgtgaagaaa gaaagcaaag
    14101 cgaaggtaaa tattttcaat tctatcaaag tagaacatgg cgaaaggctt catacttgta
    14161 tcggttaaat catccagtat gtgaggattg cctagaagaa ggcttgataa ggaaagctga
    14221 tgttgtagat cacaaaattg aactaaaaga tgattggaat agacggttag atgaaactaa
    14281 ttttcgttct ttatgccatg cacaccataa ttccaagaca gccaatgaaa gacaaagacg
    14341 agaaaagagt accctttgag ttaagggggc atagtgtgaa acttactgac aatcgatgcc
    14401 tactcatttt ggtacaaata accgttgtga aaaggcataa gggtaattac aaataacgat
    14461 tacacttgta atcacaagtg aaaaagtata taatgaaagt aggagattta ttgaaaaata
    14521 caattgttat tactgataaa acagggaaac agcgcaccat tgagttacct cattttggtc
    14581 aagtgaccat tcaaatgcag aacggaaaaa tcatttatat agataaatta gataaagaaa
    14641 aattctgatc gaaaaacgaa ggaatgtgag cttaattgct tacgttcctt tttcttttgt
    14701 ctgaaaggag gacaacatgg gacaccctaa aatattacac gatacaaaag gcaatatatc
    14761 aagtgaagaa aaagcaatcc gtgtggacgc tagagaagaa ttgttcaagc agcaaccatt
    14821 aataaatatc acgccccctg actggatggc agcgagcgct agaagtgaat ggaatcgtat
    14881 cgtaccaaca ttaaaaaaag attatccatt gagtgaagca gattatggtt cattggtagc
    14941 ctattgttta gcctttgctc gaatgaaaac agccgaagcc gagataagaa aatcaggtac
    15001 gtttatcaca tgcgaaaacg gagtaaagaa agccaatcca gcggttcggg ttcaatcaca
    15061 agctatgagt gatttgaaaa aacaagccac ctcactaggt atgaccttag aatcacgatc
    15121 aaaactagct ttgaacaagg ttaaaaatga tgaacccgaa gacccgttca aagagttgat
    15181 gaattcatga atgattacat tgagaaagtc ctatcaggta agctgattgc acccaagaaa
    15241 attatccaag cgtgtgagcg tcatataagc gatttggagc gttctaagtc agatagcttc
    15301 ccttatgtgt tcgatgaaga acaagccacc aaagcgatta aattcattga gttgctacca
    15361 tctacggacg gtaaagcaat caagatgtta ggatttcaaa aattcattct aggcagtctt
    15421 tatggctggc gtactaaaga agggaattac aggcgattca atcgagcgtt taccagtatg
    15481 agccgtaaga atgggaaaac gtatatcgca agtggcatgg ctgccaatgc gttgattatg
    15541 gaacaagaac cagcagaagc aaggcaagta ttgtttgtaa gtaacgcttt gaaacaagct
    15601 aaattgggct atgatatgct gtctaattca cttagaaacg tggtcaagtc tagtaagttt
    15661 ttaagaccac aactgaaaat tatgaactct aagattcagc acttgccctc taattcgttc
    15721 gctatggcac tggctagtga aaccagcacg ctagatgggt ttgcaccaac aaccgcaatt
    15781 cttgacgagt ggcacgaagc aaaaactcgt aaaacgtaca acgtcattaa gtcaggaatg
    15841 acccaacaaa agaacggctt attgtgtgtt attagtaccg ctgggcttga tttaaacgtt
    15901 cctatgtacg aggaatactt attgttagat cgtgtgctaa aaggcgaaga acaagccgac
    15961 aggtacttta tagcgatatg ggaattggac gatcccgaag aaattcacga tcaagagaaa
    16021 tggatcaaag ccaatccgat ttttgaaagt gaagaaatca aaaaagtaat gattccaacc
    16081 attcaagatg atgtgaacct tgctttgaaa caaaataacc ttaattctgt attggtgaaa
    16141 aacttcaatt tatggagaca agcgagtgag gacagctata tgattgctga agactggcag
    16201 gcaaccgaag tagaaccaca agatattaca ggcaagcccg tttatatcgg agtggattta
    16261 tctaaaacag atgatttaac tagcgtttca tggatcgtac cgctagataa cggcgaactt
    16321 tattgtgatt cacatagctt tgtagccacc aaatatgggc ttcaagacaa agaaaagcgc
    16381 gacggtttgc cttatcgaga actagaaaaa gctggtgagt gttccattac tcaattagaa
    16441 agtggaatcg tggactatga ccaagtattt cagtttattc aagatttgat tcaagaaaat
    16501 gatttggaat gtatggggat ttgttatgac ccgtataacg ctaattcgct tatcagtaaa
    16561 gctgaaaaag ccaactaccc aatgttagaa gtaagacaag gaacgattac tctaaacgtt
    16621 ccgacccgaa cttttagaga acaagtttat gaaggcaacg ttattcacaa taaaaatacg
    16681 attctcaccc atgcagtgaa caacgctatt ttaaaaacgg ataacaacgg cattcagatt
    16741 aataaatcaa agaacagtaa caaaattgat ccaatagctg cattaatcaa tgcctatgtg
    16801 tttgcaatgg attacttcac cacaacggaa ggagcgaaag cagacaatga attttataca
    16861 agtgaagaat tttctttcta attacattca taccgttctt ttacttcttg gattggtgtg
    16921 tgtgttggtt gctatcacct tactaacaaa tgtctactat ggcttgttag cgctgggcat
    16981 agtgcttatt ggggtagcgg tcatgctaaa tacagaacag aagggaggtt aaaagatggc
    17041 attttttaaa gcgagacaaa atacaacggg agatcctttc ctacaacatg tggtatcaat
    17101 ccaatcggat gattacacca ccagctttac aagcgttcgt gcattaagaa atagtgatgt
    17161 gtttgcagcc gttcgaatta ttgctagtga tattgcttca agtccgattc aattggtcaa
    17221 aaacaatatg ccacaagctg ataatgaact ggtgaagtta ctaaacgaga aacctaattc
    17281 agaaatggat ggttggcatt taaagtttgc tttggcggtc aatatgttgc taaacggtaa
    17341 tagctttgca gagattaaac gcaacggtga aaaggtagaa gaaattcact tattacctaa
    17401 ctcaagtgta acggttactc aattagataa tggtacgttg tcttatcaga ttggtgataa
    17461 aaaaagacgt gtgaagtcta gcgatatttt gcacttcaaa tatttcaccc aagatggttt
    17521 aacaggatta ccaccacttt atgcgttacg tgatgaactg aaaattcaac aagctggcaa
    17581 ccgcacattg cacaatttct ttactcgtgg tgtcagtggg tcaggtattt tgaaggttca
    17641 taagtcagat ttagacggat cagcaaagaa cgcaattcgt gaaaagtttg aagaagccaa
    17701 cggttcaagt agtggagata atgctcttag aacgatcatt cttgatgaaa caatggacta
    17761 caaaacatta gaaataaaca ccgatgtttt gaagcttatc aattccaatg attggaacac
    17821 aaaacaaatt gccaaggcgt ttggtgtacc gattgagcgt ttaggcgttg aaaatgaaca
    17881 ttctagcacc gttcaaagta atctccagta tatccaaagt acgctgattc attactttaa
    17941 tgtgtttgtg agtgaattag atacgaaact tgaaaccaat atccgcttta attccgatca
    18001 gttactagag actgatcctg aaacaaaagt aaaaaatgta ttggatcagg tcaaagggtc
    18061 acttctcacg attaatgagg ggcggtcgaa aatggggcta ccccccatgg atggtggcga
    18121 tcgtttacta gcaagtttga actttacgta tttagatacg ttggagaaat atcaattaaa
    18181 agaacaggaa ggagttacac cagttgaata atgaagaaga aaaggaaaaa cggctgacag
    18241 aagaagctga gctaacagcc gattctccca aagtagagaa agaaaatgaa aaacaaccaa
    18301 cagacggcaa aactatttca ggctatgcgt tgaaattcgg gcaaccgtca aaagatttag
    18361 gcggctttgt agaagtgatt acacccgaag cattaaaaga ggtggattta tcaaatgtgt
    18421 tcttattata tggacacgat tattcaaagc cactagctag tgtcaaagca ggtacgttga
    18481 aattgaatgt cgatgataca ggattacatt ttgaagcaga gctaaccgac acgacctatt
    18541 caaatgatgt gtatgagaat atctcaaaag gggttgttga ttctatgtcc tttgggttcg
    18601 tgcttggatt agattcattc gacaaaaaag aagatggcac aattgaacga tcaatagata
    18661 aaatcaaagc acttaatgaa attagtgtcg tgaccgttcc cgcttatgat tcatcaaatg
    18721 tccaagtaaa taagcgttct tacgaatcgt ttatgagtaa caaccaagca aaacaaacaa
    18781 acaatagctt agaatccact tctaaagcac aaaaggagag taataacatg gaaaaaactt
    18841 taatcgataa cgaaaaaaca gaattgcgtg ggtatgaaga atatatccgt tcacaaggcg
    18901 aagtgcgtga tggagtcact actgtaaatg cagcggcagt tgttcccgaa gaagtaatcg
    18961 gtgaagtctt tgatttgaaa cgttcaaatt ataacttagc tcaatatgca acagtaaaaa
    19021 cagtatcaaa tggacaaggt aaatatccag tagcaactaa ccaacaagca gtgttagcaa
    19081 caaaagctga acttgctgaa attggtgata ttgacgctga aatgtttact tcagttgatt
    19141 ataaagtaga aactcgtgct ggtaagattg ccttatcaaa tgaggttgtg gaagattcag
    19201 cagtgaatat tgtacaagag gtcaaagatc agttagcgaa attggtagaa aacactgaca
    19261 ataagcatat catggattta ttaaaaacat tcactaagaa aacggctgct acgttggacg
    19321 atttgaaaca actatacaat gtggcattag acccagcatt aaataaaatg gtaattctaa
    19381 accaaagcgg atattaccac ctagatacat tgaaagattc agatggacgt tacattttac
    19441 aacccgatgt gacagcacct agtggtaaat cattattcgg tatgccagta gtattgattg
    19501 cagatacatt gtttgccaat cctaaagctg gaacattccc tatgattatg ggggatattg
    19561 cacaatctat ctttgttgct cgtagaaatc aagtaacgac tcaatgggaa aaattcgatt
    19621 actactcaca aggacttgca gtgatcgttc gcaacgatta taagaaaatt gatcaaaatg
    19681 cttcagtgta tattgagttt acgccagttg taacaccaaa agtatagaaa aatattgggc
    19741 ggcggtttat cccactgcct ttttttcatt aggagagata aaatggtaat tttggatagc
    19801 ataaaaaaaa gtatgcggat cgatcacact attgatgatg actttattca acaattgatt
    19861 gatacagcag gcgaatatat aaaaagtgct attgatagta gcgcaacgga taaagatatg
    19921 gataattatc agcaatttga tttggcggtg tcattactta ctcaacattg gtatttgaac
    19981 cgtcaagaag ccagcagcga acggatacca gtaacggtac aagcattagt acaacaaatg
    20041 agaggtgctt attatgccaa tcattaagaa tgtaaatgaa ttgactgaga gagtcaagtt
    20101 taaaaagaca aaacgggtaa aagatgaaga tgggcagatg gtggatagtg aagaaactgt
    20161 atttgaatgt tgggctaatg tgcgttcaca aatgctaaaa gacgtgcttg ctagtgtagg
    20221 taccattctt gaaggaacgt tgacgtttat tattcgatac gatcaagatt atgaactaac
    20281 aaacgatatg aaagtagctt ggaaaaacaa gagttataaa attatttcaa tcaatgaagg
    20341 aacggcgttt aaagattata caacgatcat agctaaacag atttcttaga atgattacac
    20401 ttgtaaacgt tgtgggtatt tagtataatt aaggtagtaa atgaagggtt agctgcctga
    20461 attttgcaag acttagctag tcgaaatcta ttgttggact gaaaattgta ggtgtggttg
    20521 caaacatgct ggactaagaa agatgataga tgatgaacgt tcaattttaa tacaggcatg
    20581 ctaggactga ttggatggtt ttggcaagct gattgtgtag aaatcaaaga acacgtctgt
    20641 tgtggacgtg ttttttttgt tataattcaa aagaggtgat ttaatggaca aagcagagtt
    20701 tacaaatttt tattggaagt attatctaca tttagaaaat gagtttatta ataccacaga
    20761 ttttgtgatg ctcgatgaga ataatttcaa aacattctct attgaatatc aaaaactact
    20821 actagcgatt ggttcggagt gtgagattat tttcaaagaa ctttgcggat ttgatagcag
    20881 tagcaataaa agcataactg attttaaagc tgttattaaa tctagcgaat taatgtgttt
    20941 ggataatggt gttagggtta tgaattcttt aacattgact tcactaaaac cttttggaga
    21001 tgggtggcct gaagatacac cagattggtg gaaaatatat aacagagtaa aacatggtcg
    21061 ttcttcaaat tataaggaag caaatttgga aaatgtgttg tatgctttag caagtctata
    21121 tttattagag gaatatttgc ataagaaagt aatactagaa ggtgaagtag actttataag
    21181 tccagagtca aatctattta cgttatcttg ggaaagaaaa cattcgtcga tgcaaaaagt
    21241 gacgtttgaa aaagtggatt caaattatgt agctccactc attgatttta aagaacaaga
    21301 agaaatttaa aaacccccac ggcttattga agtttttgta tgccatattg tatgccattt
    21361 ggtttaaatc gcttgaaaaa tagtgataga taactctaat tcatttagtc ataaacgctg
    21421 ttttgataac ctttatagtt ctttgatatg ggtttctcat tcatggcgga agaagaagaa
    21481 tagaacaaaa gaatgaatta ataagatata aaaaaaggga ttgtgacaaa cgtcttgtca
    21541 caatcccttt ttccaaataa acgatgttca atcagctgcc cctcggcatt cgctcgaaaa
    21601 acggcgaaat atgaagaact attttgccat ttgtttctct aataatcagg tcaaaacgtt
    21661 gacttcacag tctcttcctt aaacggtgtt ctcctgccaa ctcctcggtc acaagtcaca
    21721 atattcaaca aacatgagaa gctgtttttt gaaattgttc cttgtgactc ggagctgaac
    21781 ggcaggccca cgacctcttt cttaaacggt gttcaaaagc ttgcatctgc ggtaataagt
    21841 tcaaccaaac gaaaattatg aggagctatt tttgtttggc tgttcttatt acttgatgca
    21901 ggacagcttt ttcacaacct cttttcgttt agcactaaat ttccttaaaa agtagaaaag
    21961 ctttcgaagc ctcgtttgtt tcttttactg tataataaaa gaaaagagta acaatcgcta
    22021 aaagatataa acaagagaaa ggaaatacaa gatgattggt ctgatttatt ttgtggtgat
    22081 cgttctggcg aacacagttg gcgctatttc cgggatgggt ggaggcgtgt tgatcaaacc
    22141 gattcttgat cttatcggcg cccattctgt tgcagggatt tctttttact caacagttgc
    22201 ggtattcacg atgtccattg tttcgactgt gcgacaagta tcaagcggga aatcactgaa
    22261 ttggcagatt gtcggttggg tatctagtgg agctgttgtt ggaggagtag tcggcaacat
    22321 tgtattcgaa gtttttttac agttatttga aaacgagaag catgtgcagc tgatccaaat
    22381 ttttttgacg gtattgacct tggtttttgc gttcttctac accaagcatc atcagccaaa
    22441 atttcatttg acgtcatgga cttggtacct tatatgtggg ggggttctgg gctttttagc
    22501 tagtttttta ggtattggtg gtggaccagt caatgtttct ttgttgatgc tgatgtttgc
    22561 attgccaata aaagaagcaa cactatattc tctatctacg attttctttt ctcaactggc
    22621 aaaattggta acgattgctt taacaagtag ctttatgcgc tttgatttat ctatgttgtt
    22681 ttatgttatc cccgctgctg ttattggagg tttgtgggga gcaagattta gcagggtttt
    22741 gtctccaaaa aaagtgacct ttatttttca agtaattgtc ctcgtggttc ttttggtcaa
    22801 ttgttacaat gcctttgtca tcttacaaac attaggagaa tctagactaa cgcaagtcaa
    22861 aatcactgaa acatattgtt ctaaatgaaa aaaagagtat actatgataa aaaaattcat
    22921 tttaaaaaat cagaaattgg ctggtgattt ggatgttacg ggaaatgcgt ttagaagatg
    22981 cagaagcact acgtgaaatc aatgcggatc aattagggta tgatatacca ttaaccgtta
    23041 cggtgagaca aatgaagaaa ctgctgaagg agccagagaa aaattttttc ttggtttatg
    23101 aagatcctac ttctaaaaaa gcagctggct atgtacaagc ggagtcctat gaatcgattt
    23161 ttagcgatcc aatgttcaat attatggcac ttgctgtgtc taaatatgct gaaaaaagag
    23221 gcatcggaaa ggctttgatg actggcgtag aagaagaagc gaaaaaacga ggaataacga
    23281 caataaggct taattctgca gaatacagag tagaagcaca ccaattttat gaacacatcg
    23341 gttatcattc cgataaaatg caaaaaagat ttttgaaaat cctgtaaatc tttatcaaaa
    23401 aaatacccga tcaccaagcc gatacttgat gatcaggtat ttttgttttt ttgactaaga
    23461 aagtaattgc atcaagaaac cagagatgat caaaacgatg gcaccaccaa gtcgattgcc
    23521 catttgagca aaagcaataa gttccatccg attagctgca gataagacgg ctacatttcc
    23581 tgttccgccc atcgagttgt tgcataagcc agcagtaata gttgattcaa ttggatataa
    23641 accaaacaaa cgaccgacaa atcctgaaac aactgaaatc gcgataacac ttgtcagaca
    23701 taagacaaca aattgccatg tcaaagcaga agctaataca ttcagattca gcaacgcaat
    23761 accgataccg gcaagaacag ctgctgtcaa gtttttgaca atcaagttat tgaacatgat
    23821 agctgcatct tcataatact ctggtaatag gttggcgatt ttacagatga caacgataat
    23881 aatcataaat gcgtaagcat ggactttagg aacaaaatag ttaccaattt gtcctaagat
    23941 gaagaaagtc aatgatacca tcaaaccaac acctaactgt ggaatggaag gtttcacagt
    24001 tcgaggtttt ccatcgccaa gatcgccttt ttccattaat ttaccattgc cgttgaattt
    24061 tggcattgat tgtccagcac gagcaactaa tgctgcaccg atgatagcta agacatttgt
    24121 cattgctgtc gcaggaatca ggcgggaaat gatagaagaa gcatccgtac ctaaaacatt
    24181 tgcataaatc gttgataaag gaacggctcc ggctccaaca ccaccagcca tcgcaggtaa
    24241 tgagatgtat aaaacggaat ttgcaaaacc atttccgata agcatgccca cgagtcctac
    24301 ggcaaaaaag gagacgacca ttgaaataag tgcaacggga ataaaacgaa cagaagcttt
    24361 catcaataga ttacggttca taccaaggat gctaccagta atcaaggcag caatataaaa
    24421 gtcccaaaag cccatattgc tgacaaagtt ttctgtggat ttcactacat attcaggtaa
    24481 gatcccaaat gtagcaatcg cagcagaagc gaagatcgca aaaacggaac caccacccaa
    24541 ataacttttg acgataggga tttttgtccc gataaaatga aataaattac cgaaaataac
    24601 caaaactgcg attggtccaa tcatattcgt cggtaatttc cctaaagcca ttgcaatgat
    24661 caggacaagg accatgatca aataaagtgg caaacttacg ccggtgattt ttgtggacca
    24721 aaaaccttct tttttctttt cctcaactgc agttgcagtt gttgtttttt gtgccatttt
    24781 tacagatcct ttcttattct gtatcatatg aaaaaagcca aagaaaagcg acaaaagagc
    24841 aggagttggc tggccgctgt tttgccgcag tcgctttttt ctttttagac gtcatacaaa
    24901 gtttctgtat gttcttttac tataacgatc ttaatcttct aaagatttat attcaggaac
    24961 ccatttagca gattcaactg cttctttgat atccgtgatt tcttcacgat tcaagttttg
    25021 tgcgacaact gattttgcga cagtttcagc aatcgtttga gaaaattcag tgatttttgc
    25081 tactggaggc aagatagctg ctcctggttt tgttacatca acgattccgc ctaatgctcg
    25141 actagcttga gaaatgattt cagcatttac tcgtgtagct gttgaagcaa tcaaaccaag
    25201 tcctaaacct gggtacatca aagcattatt tgcttgaccg atttggtaag ttacaccatt
    25261 atattcaaca tcagcagcag ggatacctgt tccaactaat gcttttccgt ccgtccattc
    25321 tattaaatct tttgcttttg cttctgccaa ttttgttgga ttagacaaag gcatgatgat
    25381 cggacgtggt gtatgagcag ccatttcttt cacgatttct tcagtgaatg ctccaggttg
    25441 ggtagaagta ccaatcatga ttgtcggatg gatcgctttt acaactgctt ctaagtttgt
    25501 caattcatca gcattagtga aatctgagcg tttgcgagcg aatgggattt gaccaggagt
    25561 caatccttct gtatcttcaa acaataaacc ttgtttatct acttgataga aatgttggcg
    25621 tgcttcttct tcaggtacac cttgacggat catctcatct aaaaggatgt ttgcgatacc
    25681 cacaccagca gtaccagcac caaatgtcaa aattatttga tctttcaatt gttcaccgga
    25741 tatattcaat ccgcctaata cacctgctag gacaacaatt cctgtacctt ggatatcgtc
    25801 gttgaaagta gtgatttgat cttcgtattt ttctaagatc gctgcagcat ttcctcgtcc
    25861 aaaatcttcc caatgcaata acaattctgg gaataattcg gtagctgatt gaacgaattg
    25921 atcgatgaat tcatagtaag tttcgccttc tacacgagca tgacgattgc ctaagtataa
    25981 gggattattc aataattctt cattattagt accagcgtcg atcgatacag gcaaaacttg
    26041 tgctggatta ataccagcag cagcagtata gaccatcaat ttgccgatag cgatgtcaac
    26101 accgttcaca ccccagtcgc ccatacctaa gattccttct gcatcagtca caacaatcaa
    26161 acgaatgtct cgtccatctg ccgcgttctt caaacttgct ttgatgtttt ctggttcgtc
    26221 gattgaaaga aatgcagcat cttgcggttt caagaaaatt tcgttgtatt gttcgattga
    26281 atccgcaacg acaggatcat aaacgacagg catgaattca actaaatgtt cgcccatcaa
    26341 tttataaaac aatgtccgat tcgtattgaa tagattcatt aagaagatac gtttttccaa
    26401 atctgtttgt ttgcttaagt attgaccata agcttgaaca gattgttgtt ccagtgtttg
    26461 gacagtactt ggaagcatac cagtgattcc atattttgcc cgttcttctt ttgtaaaagc
    26521 tgttccttta tttagaaaag gattatttaa taagtttaac cctgtaagca tgaatgattc
    26581 ctcctaagtt ttaatacaaa cgaagtatag aagtcagatg atgatatagt caaaaagagc
    26641 aggtatgata aattttattt atatgtaata tagccaaaag aaatcaaaat agcagtattt
    26701 gttcttttta tatcgacaat ccttgcttga atactatgcg tatctcttat atgctagaga
    26761 aagaaaagta gaagaataag ggatagatgg aatggataaa taggatttct gaggaaaaaa
    26821 gcacatgata gcgttgatag gagtcaccta tgtcaggtga taaaaataca taagtagaaa
    26881 attaagagga ggaagagaaa atgaaagaag ttcaattgat tagaaaaagc gaactatcag
    26941 aaggcggttg caatgcatgt ggcgtagtag aagcgacaag ctatacatta aaattagatt
    27001 ccaatcaagc aataatctct gaactgacag taggcggtct tgtggacagt ctggcgcttg
    27061 ctgaaggttt tactggagaa gatatttatg agatgttttc agaagtccgt cagttaaaaa
    27121 aaggagaaaa atgcatagag gttcatcatg agagcccgaa tgtgaggttc aaacgtggtg
    27181 ataatgagat gatcttcaag aatcatgtgt ctgatcacac tgaactatat gaaatcgtga
    27241 atcaaatact gtctgggatt tttgaattag gaccttatga gttcaaggaa gaaaacggta
    27301 atccaaaatt aaatgaagaa tggcaagaaa cgattgaaac acaaagaaac aatccacact
    27361 tatttcaata gtcttccaca gtatttcgta aaaatgttga tgaaaaagaa acaccgtgaa
    27421 tcagaggtcg tgaaaaacct gccctgcatc aagtaataag aacagccaaa caaaaatagc
    27481 tcctcctatt tttcgtttgg ttgggcttat taccgcagat gcaagttttt gaacaccgtt
    27541 taaggaagag actgtgaagt caacgttctg acctgagtat tagagagaca aatggcaaaa
    27601 tagttcctca tattttgcca ttttttgagc taataccgag ggtcagctga ttgaacaccg
    27661 ttttatcgag aggttgtgaa aaagtcgctt agtttcgagt agtaagaaaa attttgtgaa
    27721 aatggctttc cacatttttg cataaatttg acttaatacc gagaaaatgg cttttgaaca
    27781 ccgtttattc aaaaaaaggg actttgacac tacttttgtc aaagtcccat cggtttttta
    27841 tctattttta ttacaatcat ttcttccaga gtagtgacat ctggaatggc tgaaatgtaa
    27901 acagattatt tgcctgtatc aatgtcgaag agtaatataa aaagtgtaaa cgctatcata
    27961 ttgttcatca ttcaaatttt atagagagaa ggcattgaga tggaaaaaac atatcaagca
    28021 ggtacaaatg aaggaatagt agattttatc aatatggaag atttagaaat cgcggcttca
    28081 caagtgattc cagctggtgg ttatggttac atcagcagtg gtgctggcga cttatttact
    28141 tatcaagaaa atgaacgggc attcaatcat cgtttgatta ttcctcatgt attaagagat
    28201 gtagaattac ccgatacaac tacacatttt gacgaagaaa tgctgactgc accaatcatc
    28261 atggcaccag ttgctgctca tggattagct catgtgaagg cggagaaagc ttctgcaaaa
    28321 ggtgtagcag attttgggac gatctataca gcaagttctt acgcttcttg tactttagaa
    28381 gagataagag aagcaggcgg ggagaaagca ccgcaatggt ttcaatttta tatgagcaaa
    28441 gataatggaa tcaatttgga tattcttgaa gtggccaagc gtaatggagc aaaagcaatc
    28501 gtattgacag cggatgcaac agttggcgga aaccgtgaaa cagaccgcag aaacgggttt
    28561 accttcccgc tgccaatgcc gatcgttcag gcttatcaat caggagtagg ccaaacgatg
    28621 gatgctgtat ataaatcttc caaacaaaaa cttagtccaa aagatgtaga atttatcgct
    28681 gctcattctg atttgccagt ctacgtgaaa ggtgttcaat cagaagaaga tgtctatcga
    28741 tcattagaat ctggtgctgg tggaatttgg gtatctaacc atggtggccg tcaattagat
    28801 ggggggcctg ctgcgtttga ttcgttgcaa tatgtggcag aagcagtaga taaacgtgta
    28861 ccaatcgtgt ttgacagcgg tgtacgacgt ggacaacatg ttttcaaagc aatcgcatca
    28921 ggtgccgatc ttgttgctat tggcagaccc gttatttatg gattgtcttt aggaggaagt
    28981 acaggagtcc atcaagtgtt tgatttcttt aaaacagaat tggaaatggt catgcaatta
    29041 gctggaacac aaacagtaga agatatcaaa aaaataaaat tgcgtgagaa tcgttttatt
    29101 taagaaataa atgacgaaaa aaataggagt gcggcagatg atgccgtact cctattttta
    29161 tagttcaagg atgtcactaa tagaataatt tgaaacgaaa aaataaaa
//
LOCUS       DAXWOO010000014        29194 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.014, whole genome shotgun sequence.
ACCESSION   DAXWOO010000014 DAXWOO010000000
VERSION     DAXWOO010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.014"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(188..2323)
                     /locus_tag="ACGE70_000524"
     CDS             complement(188..2323)
                     /locus_tag="ACGE70_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFF8692186.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(2406..4061)
                     /locus_tag="ACGE70_000525"
     CDS             complement(2406..4061)
                     /locus_tag="ACGE70_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFF8692187.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(4063..4377)
                     /locus_tag="ACGE70_000526"
     CDS             complement(4063..4377)
                     /locus_tag="ACGE70_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFF8692188.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            4547..4807
                     /locus_tag="ACGE70_000527"
     CDS             4547..4807
                     /locus_tag="ACGE70_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFF8692189.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            4821..5135
                     /locus_tag="ACGE70_000528"
     CDS             4821..5135
                     /locus_tag="ACGE70_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFF8692190.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(5865..6419)
                     /locus_tag="ACGE70_000529"
     CDS             complement(5865..6419)
                     /locus_tag="ACGE70_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFF8692191.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(6545..8474)
                     /locus_tag="ACGE70_000530"
                     /pseudo
     CDS             complement(6545..8474)
                     /locus_tag="ACGE70_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(8780..9496)
                     /locus_tag="ACGE70_000531"
     CDS             complement(8780..9496)
                     /locus_tag="ACGE70_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFF8692192.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(9497..11320)
                     /gene="glpO"
                     /locus_tag="ACGE70_000532"
     CDS             complement(9497..11320)
                     /gene="glpO"
                     /locus_tag="ACGE70_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFF8692193.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(11334..12830)
                     /gene="glpK"
                     /locus_tag="ACGE70_000533"
     CDS             complement(11334..12830)
                     /gene="glpK"
                     /locus_tag="ACGE70_000533"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFF8692194.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(12966..14426)
                     /locus_tag="ACGE70_000534"
     CDS             complement(12966..14426)
                     /locus_tag="ACGE70_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8692195.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(14416..15711)
                     /locus_tag="ACGE70_000535"
     CDS             complement(14416..15711)
                     /locus_tag="ACGE70_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFF8692196.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(16043..16720)
                     /locus_tag="ACGE70_000536"
     CDS             complement(16043..16720)
                     /locus_tag="ACGE70_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFF8692197.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(16750..17841)
                     /locus_tag="ACGE70_000537"
     CDS             complement(16750..17841)
                     /locus_tag="ACGE70_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFF8692198.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(17942..18376)
                     /locus_tag="ACGE70_000538"
     CDS             complement(17942..18376)
                     /locus_tag="ACGE70_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFF8692199.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(18960..20417)
                     /locus_tag="ACGE70_000539"
     CDS             complement(18960..20417)
                     /locus_tag="ACGE70_000539"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFF8692200.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(20414..22309)
                     /locus_tag="ACGE70_000540"
     CDS             complement(20414..22309)
                     /locus_tag="ACGE70_000540"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFF8692201.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(22367..24142)
                     /locus_tag="ACGE70_000541"
     CDS             complement(22367..24142)
                     /locus_tag="ACGE70_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFF8692202.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(24192..25688)
                     /locus_tag="ACGE70_000542"
     CDS             complement(24192..25688)
                     /locus_tag="ACGE70_000542"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFF8692203.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(25882..27504)
                     /locus_tag="ACGE70_000543"
     CDS             complement(25882..27504)
                     /locus_tag="ACGE70_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFF8692204.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(27832..29145)
                     /locus_tag="ACGE70_000544"
     CDS             complement(27832..29145)
                     /locus_tag="ACGE70_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFF8692205.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
BASE COUNT         8343 a         6294 c         4885 g         9672 t
ORIGIN      
        1 ttactttttt catatcctca cctcacgttt ttttgttacg acgtaggagt ttaccataca
       61 tttcatttct tttcacataa agtgttccta aatttttcct aagaaaaaga acaaaacatt
      121 tctcatcagc aaatgaaaaa tattttgttc tttttatcag ttgttctttt tattttttat
      181 ctcttaatta tgttgctctt tccaatacga caatgctttt cattcctcta gatatcgccg
      241 tgtacatttg tctgcgtctt ctttgctcat ctccatcgtt ttcaaatgga tataagatta
      301 catgatcaaa ttccagtcct tttgctagac tcaaaggaat gatttggcag ttttctaatc
      361 catatgaatt ttttttaatc gattcttgta gcgtttgggt ttcctcttct gtttgggtga
      421 taactgccgc tttttctcct tttagagaaa ccaaaatctc agctagtatt ttcaacaatt
      481 cttcagagga tcgaaaagag cggtattcgg gttcttttcc ttgcggtcgg atcgatacga
      541 tgtcgatctt ctcttgatcc acagcatagg ttttgaaaag ctctgtgatt gctccgctag
      601 aacggtaact atttctaaga tcataccgat ggatcggcag atggtagtcg tcgaaacaac
      661 gcataatatc agcaaaagta gtggaagaat taaagattgc ctgattctcg tcacctacta
      721 aagtaaagtg agtttttgga aataattcga tcaatagacc taattgtgcc agggtataat
      781 cttgtacttc atcaatcaat agatagcgaa atgctggtac atttaccttt tcaacaagta
      841 aatgacgaat agctaatata atgactgctt catcaacagt acgtgtgggt tgccaagcat
      901 aagctgagcc atatcgtttt tcataaattc tttcaaacaa ctgatgctca tccacccatg
      961 ccatttcttc gatttgtctg gttatttttc gatatttttt ttgtaaaagt ttccttgcat
     1021 aagctacaat actttgttcc gaatcatcag aaatcaattt accgaataat tccaactgcc
     1081 tatcttctgt caacgagagt acctgattct gcacagcagg actttttgct tgtttcaaaa
     1141 gatgattctt ccattcgctc atcagtgcct ttttcgttgc ttgaagccta tcgatcaatt
     1201 taggatgagc aggaactttc tcatatattt tttcgatcgt ttttttagaa aggactgttt
     1261 ttccattttt agtaagtcca cggataaaat tcggatgatc taaaaacaaa ggatctgatt
     1321 gttttagatt atcgataaac tttttgctac gcaaacgttc tgtctgctcc gatacatttt
     1381 ctcccgtgat acgtttgaaa taagcctctt caccttctat ttcctctgct gaaagctgtg
     1441 aaaccagctg tatgatggtt agattcagtg gatttctttc acctaatgat ggtaggacat
     1501 tcgaaatata gtcgataaac cgatgattag gtgatagaat cagcaagtca tcggatgtca
     1561 tcgtatctcg atataaatag agcagatacg caatacgctg catgatagca gaggttttac
     1621 cacttcctgc tacgccattt accaagatat tttgactttt cacatcccga atgattgtgt
     1681 tttgttccga ttggatcgta gcagtgatag ccttcatttc attggtctca tcttggccta
     1741 agacttctaa taatacgtca tcttgaatag ctacagacgt atcaaaaaac ttgaccaaac
     1801 gatctctttc caaaagaaat tgtcgtcttt ctttaataac cgtatcgatt ttattttgac
     1861 gaaccgtata agaagatgga ccaatcatgt tattgtaaaa aagttcggca attggtgaac
     1921 gccaatcata aacaagattt tcatctgcat catctgtgaa atttgctgta ccgatataaa
     1981 aggattcttt tggctcatta tccagaaagt ctacaacgac ttttccaaag tagggacttt
     2041 tcaataaacg ttcgatcttt gctagctgat tttctgcgtt tttctgtttg atattcatct
     2101 gatcgatttc tctatttttc atttcaatca tcgaaaaagt atccaggtta tctaagtaac
     2161 tgtcaaaatt caatcgaagc tcgccagcca tttctttcaa cgactggttt ccttctattt
     2221 ttgccgtatc catcgattcc cgcagttcgt tttttttatc aattagcttt tgatacacgt
     2281 tcttcagata tgcttcttct aactgttttt gtgtgttgac catacacatt ctcctctttt
     2341 tttaaagtgg tggaaccgat tagtttacct cttttaagag tcgaatgcaa gctttttaac
     2401 gagtatcatt tgttaggata tatcagttct tcaggtcgga cagtttgata taaagaaaat
     2461 gcaccatcaa ggttttttac gtggtagcca gactgcttca agattctttc cgctagatag
     2521 ctacgtaatc cgctatgaca gctgacgata tattcttgtt ggctgtctaa ttcatttaat
     2581 cggtcccgaa gttcattcaa agggatatgt ttggcttcct tgaatttgcc cttttccaat
     2641 tcttctgctg ttcgcacatc taataaaatt tttccttttg ctagttctgt cggcaattca
     2701 tgccattgta cggtttcact aagcccttct gcaatgttca ttgctgcata tcctagcatg
     2761 tttactggat ctttggctga gccaaatggc ggtgcatacg tcaattctaa ttccggtaaa
     2821 tcgaagatcg tcaaatgccc tttgattgct gttgctaaaa tatcgatccg tttatccaca
     2881 ccttttgccc caacaccttg tgcaccataa atctcgccag tctctggatg gaagatcaac
     2941 ttcagtaaaa tatctgttgc ttctggataa tagcttgcat gatcttttcc tgatacatga
     3001 atgacttcaa aagacagttc gttttctttg gctgttcttt cacttaaacc ggtggatgcg
     3061 gcagtcattc caaacgttcg tataattgct gttccaatac cgccttgatt tcttcgagaa
     3121 attccggaaa tcgtgtccgc aacttgtcta ccttgacgat tggctggtga agcaagagaa
     3181 ataagtgcat cttttccagt gatttgctgt tttacaacga tagcatcccc caccgcataa
     3241 atatcaggaa tattcgtttg ataacgttcg tctactagga tacccccgcg tatgcctaat
     3301 tcgattccag cggtcactgc tagctggtta tcaggacgaa caccaatgga taaaataact
     3361 aaatcagcag ggatggtacg cccatcatcc agtcgcaatc gtttcccgtg gtcttcaaat
     3421 ccagcgacag catgagaagt aatcacttct actccttgat gagacaattc tttttctata
     3481 aaagctgcca tttcttcatc cagtgttgga agaatatgtg gtgcttgttc gatcaccatg
     3541 acttctaaac ctcttctttt taggttttct gccatttcca atccgatgaa tcctgcacca
     3601 acgatcacgg cacgctttgg ctgtttttct aatgcatgca tcactttatc gatatctggt
     3661 acatttctaa tagaaaaaac attgtcggct tctgttatcc ctggaatcga tggaacaact
     3721 ggttttgcac caggagataa aaccaatgca tcgtactgtt caatgtgttt ttgaccctca
     3781 tgtacgactt ctaccgtttt gttttcggga aagatggctg tcacttcatg atttggacgc
     3841 acatctaaac aaaaacgagc ggataatgat tctggcgttt ggacaagaag attttcccgt
     3901 tcactgattt ctcccgaaag ataataaggc aagccgcaat ttgcaaaaga aacatacggt
     3961 cctttttcaa aaacaacgat ttctgcatct tccattaatc ggcgaagccg tgtagcagca
     4021 gacattccac ctgctacacc tccgacaata acgattttca tcttatttcc ctcctactgt
     4081 tcgatcaaac cattgattca tgccaccacg tacattcact acatcataac cagatttctt
     4141 taattcttta gctgcttgtt tactcctcat ccctgattga caaatcacat agacggtctt
     4201 ttctttgtct ccatcatatc tgtttatact ttgtaatgga acatttttag ctccttttat
     4261 atgcccgccc cggtattctg caggtgttct aacatcaagt aacgctggat tttccaaaag
     4321 cttttccttt aattctggga ctgttatcga ttttattgat tgaaatagca aattcatttt
     4381 ctgcacctca tttacatttt tataaatacc tcatggggta tattaaacag gagctttatt
     4441 aatatgtcaa ccagaatgac tcttcatttt tttcactact ttttatattt gcataaaata
     4501 ccgataaggg gtattatcta attataaaaa tagaggagaa tgtgttatgg agattgataa
     4561 taaaaatatc ctgaaccgtt tgaaacgtac agagggacaa attcgtggta tccaaaaaat
     4621 gatcgaagac gaaaaagaat gtatggatgt catcactcaa ttaagcgctg ttcgttctag
     4681 tattgatcgc gtaatgggca tgatcgttgc agataatctg aaaaattgct ttgagaaacc
     4741 tgatagtaat ccagaagaac aagcggctaa actagaacaa gcgatcaaga tgattattaa
     4801 aaagtaaagg aatgatttca atgaaagtcg ttttccatat tgatgaatta gaaaaatggt
     4861 cagaaacagg gaaaaacgta aaaaacctga taaaagcttc tccagaaacg acgattgtcg
     4921 tatctgtaaa tggcatcgcg attaccggtt atctagactc tgccaatgca gaattcttag
     4981 atttgcaagg agtcgttttt cacgcttgcg ccaatgctat gcgtgctaat catatttcag
     5041 aaagttcttt acctgaacaa gtaatcgttg taccagcagg tgtccttgat ttagttgaac
     5101 tgcaatccca aggttatgct tatatcaagc cttagtcagt agaggttgcg acagaagtga
     5161 tcagcttcaa gaaatttcgg cttatttttc gaagaagcta cttctggaaa accgtgaatc
     5221 aaaggttgtg aaaacgctgt cctgcatcaa gtaataagaa cagccaaaca aaaatagctt
     5281 ctcatatttt tcgtttggtt gggcttatta ccgcagatgc aagcttttga acaccgttta
     5341 aggaagaggt tgtgaaatca gcggtttgac ctaagtatta gagaaaaaaa cgggaaaata
     5401 gctcctcata ttttgccgtt ttttgagcta ataccgaagg tcagctgatt gaacaccgtt
     5461 taaggaagag attgtgaaaa agtcgcttag tttcgagtat taagaaaaat tttgtgaaaa
     5521 tggatttcta catttttgca taaatttggc ttaataccga gaaactggct tttgaatacc
     5581 gtttaaggaa gaggttgtaa aatcagcggt ttgacctgag tattagagaa acaaacggaa
     5641 aaatagctgc tcatattttg ccgttttttg agctaatacc gaaggtcagc tgattgaaca
     5701 ccgtttacca agaggttgtg agcctaccat ttaactccga atcacaagaa ataatttcaa
     5761 aaacaacttc tcctgttttt ggttcggttg gcttattatc gagaagtagc tactgggagt
     5821 ctatttatca aaaaaaggaa tgcgacatag gtcacacccc ttttttattt tctttgaaac
     5881 atctttcggt actcgacagc atacaagaca aaagattgta aaagaccgta agtgatgacg
     5941 gcaaaaatga ctctcaccca cagcgttggt aattctggga aaagcagcaa aaggatcatg
     6001 ccaacataga tgacagcagt cgctactttg ccataccact tcgctccact taatttgata
     6061 tttttagata gcccgattaa tccagctaca aacataaagc cttcttttat caagaataga
     6121 ccaaacaaca gccaaagcca cggtcgatac catgccatag acaaaatcag aaaaagttgt
     6181 gtcaatttat ctgcaaacgg atcaagcacc ttccctaagt ctgttacctg attatatctc
     6241 cgagcaatga ccccgtctag ataatcagac aaacctgaga caacaatgac cgctcctgct
     6301 atataaaatg actgtctcag cacaaaataa agataaatcg gcagcaaaaa aatacggaat
     6361 atcgataaaa gattcggaat cgtttgtatt tctttacgcc agtctgtgct taatttcatt
     6421 ttatcgccct acctcctcag cactcttttc tttttatttt agcttacacg gggaataaaa
     6481 ccaatgattt cactgttttt atttttcttt acagacactg atattatgag ctaatttgag
     6541 ataatcactt gctgttttca tcgtttactc ttctttcttc ctgtcgtttg cgcatttctt
     6601 ttactagtac tttatgagct ttttttgatc ctcgctctag gtcacgctgc cgtttacgtg
     6661 ttttataatc gataaacaga ctgtctaaat cgtaacctgt cgggtacaga tcttctgctg
     6721 aacgcaccag acgaacacgc tgacgaggca cgtggattat ttttttgtct aaatatacat
     6781 caatcgtatc ttcacctata tcttcataaa ctaacgcttc tttttggtat tcattgacaa
     6841 atatccgatc accttttgca aagagctgac gttctttttt attctccttc ctatttgtaa
     6901 ctgctgtctt aggaagaaat tcttttttct gtgtccgata ggctttttcc gccaagtaac
     6961 gctctgcttg atggatcaat ttcatcgaca tatccatttt atgagcaatc cacaaagctt
     7021 ggctttctcc tgtttcttcc aacccgcaac tgatatttag gacgaagtgc ttcccgatcg
     7081 aaagccatag cagccggcac aaaatcttca tgatctccgg cgaatttctt gatttctcca
     7141 taatgggtcg tggcaatgat caatgcccct tgctcataca tagattccat gattgcgatc
     7201 gctagtgcag ccccttcatt tggctctgtt ccactgccaa tctcatctaa aagtaccaac
     7261 gtattccttt ttatcttttt caaaatagca gcgatgtttt gcatatgacc agaaaatgta
     7321 ctcaacgcat tttctaaatt ttggtggtca ccgacatcga cgaaaatctc atcaaagaca
     7381 gcaagctctg tcccttcttt tgccggaact tgcataccga acatcgccat caaagtcaat
     7441 aaaccgactg tcttcaacac gactgttttg cctccggcat ttgctccggt gataatcaag
     7501 cctcgatagt ctttaccgat ttcaacatcc aatggtaccg catgctcagg taaaaatgga
     7561 tgtctgcctt gcttgataat gatatgttcc gatttattga ttctaggtgt gcttccattg
     7621 atttctcggc taaattttgc ccgagcaaag atgatatcta acaccgtgat cgtttcgatc
     7681 aataaatcga tcacttcttc gttttctgcg atagcaccag tcaatgccgc taggacttga
     7741 tattcttcac tgatttcttc agcttttaac aactgatagt gttcattcaa tttagaaatc
     7801 acagttggtt caataaaaac cgtcgttctt ttatttgatt catcgataat cgttccatct
     7861 attttatttt tatagctggc tttgatagga atcgtatagt attcgccttt ttgaacaatc
     7921 attgcttctt ggatcatttc tttgtttttc ggatgacgca agaatttcag taattttgac
     7981 tggatttctt tttcgattag ttgtaattgt ttgcgtactt tacgcaaatt acgtgacgcg
     8041 tcgtcactga ctttattatt ttttattttc tggtcaatca actcttctac atttatcaaa
     8101 tcaggaagat gtttgctata agcaaacaac aagggtgtct ggtattgatt cttatcaaaa
     8161 aactttgtga tcatccgact gcttctaaga aaatccgcat attcgatcaa atcggttggc
     8221 tgcagaatca accctttttt gacttgttct gtcagtgcat cgatcctcgg caaccccata
     8281 aatggaacat gctgactgct ttctaagatc aagcgtgctt ctcttgtttc ttcttgacga
     8341 tccaagacag tttgtaggtt tgtcgagacc gtcatatcgc tgattcgttt tttgctatag
     8401 tttccaattg ctcttgcttg gatttccttt ttgatccgat caaattgtaa ttgactaatc
     8461 atttcgttgt ccatattctc tccttttctt gaagagatac ctgtcccaaa ataaataagg
     8521 gcctagagat aaataaaagg atagaacaca gcttaaaaaa gtcatgtcct atccttatta
     8581 gaatacaaag atattgtttt cctgacaggt accgtttcat aagcacacca aataaacttt
     8641 gtttattggc tattcgactt actgattaga taccagctac actcacaaaa cataccccat
     8701 tcttctaggt cttatttatt ttccacttaa ctatagcaag acacattttg gttgtcaaac
     8761 tttagtatgt tttatagatt tacaccatta atgaatagac acctaccgcg ataattgctc
     8821 cgatcattgg acctgcgatt ggtacccatg aatatgccca atctgaagtt cctttattag
     8881 ctattggcaa taccgcatgt gctaatcgag gaccaaagtc ccgagcaggg ttgattgcat
     8941 atcctgttgg ccctcctaat gaaagaccga tggctaagat caatccacct acagcgaata
     9001 cgttcgttcc aggtgcaaat tcgttttgtc caaaagctag caacccaaga actaatacaa
     9061 atgtaccaat taattctgtg atcacatttg ctggataatt ccgtatagca ggacctgttg
     9121 caaacgttcc taaaatcgcg ctttcatctt tcgttgcatt ccaatgtggc aaataagaaa
     9181 gccaaacaag aatggcccct gcaaaagctc ctaaaacttg cgcaatgatg aaaggaacga
     9241 caagattcca actaattgcc cctgtcatcg ccatagctag agagactgca ggattcaaat
     9301 gagctgggcc catgaatcca gaaatataga cagccattgt tacagcgagt ccccaaccaa
     9361 atgcgatgac aatccagcca gaagcctgtg ctttactttt gtttaaattc acagccgcac
     9421 acacaccatc tccaagcaaa actaggatca gtgtcccaat aaactccccc atgatttgag
     9481 tcatatcact gttcatttat ttctccccct ttagttcccg cagatctgat tctgcaatca
     9541 atgcttctaa acgtttagtc tgttgcgcct tctcctcttc tgtccattca aaatatccag
     9601 caattgcatc gatcacaggt tgcttgatct catcaagctg atctcgttca aacaacagat
     9661 gatttgtacg acgaatgaga taatcgcctg gggccaaaac catttcttct tctaaaccat
     9721 aacgtaaccg agctgactca gcgagactca aaccaggata aggtgccatt tcttttgcta
     9781 attcaaagat acgtcgggca ttagtcccgt aaaaatcagc gatataagta gcatcttctt
     9841 cctctaaacc ggctgctact ccttcttttg ctaattctgt cactgtttct tctaatttag
     9901 ttggatcgaa gtttccacct gaaatctgat attttttaga gtcgatttct ttcgtctcta
     9961 ttccgtattc ttcttttaat aattggcgta tcaatcgcaa agctccttct gccatcttac
    10021 ggtaatcggt gattttccca ccagataaag tcagcaaacc atctggttct ctttctaaag
    10081 aactacctct ggaaatcgta gaaggtgctt tttcatcacg gctgttttcc aaatggttca
    10141 acacgtcttc tacttcagca cgagaaactt tgttttcctt atattcactt actgtatcga
    10201 caactttatt gaagctcttg tctgaaatcg atccattatc tccaccattg taatcagaac
    10261 cagaattacc aatcaacagt ggacgaagtc ctgcccagct tgcttcgata tcattcaatg
    10321 tgatatttgc ttctggatag cgatgattga tcacatccaa tagataatct acatcttctt
    10381 gtgtgacttt cggatccgta aaatctcctt ggtaatccgt atctgtcgta ccaaagtaag
    10441 tcttgttttc tcttggaata gcaaaaacca tccgcccatc ttgctttcct gtatcgaagt
    10501 atgtgggttg cggtacaggc agttttttcg catctacaac taaatggatc cctttagttg
    10561 gacgcatttt aggagagact ggacgcgtaa aattcaagtt tcgtacttta tctacccaag
    10621 gaccactcgt attgatcact aattttgctt tgatttcgat cacttcatct gtcagcaggt
    10681 cacgagcttt gacgccaacg atttgatcgc catcatataa aaagccaacc gctttcattt
    10741 tacttactag ataagcccca tcttcggcag cctttttgat attatcaatc actaaacggg
    10801 catcattgtt gcggaaatct agatagacac ccgcgccttt tagcccttct tttttcaaaa
    10861 acggttctct ttccaatact tcttctggtg tcagcgtata attctcatat ttggttcctg
    10921 tcacatttgc cagtttgtca taaaggtcca ttgctacttt gaccgagaac atattgaagg
    10981 ttgttgctcc ttcgccctca tagattggca aaagcattgg atctggtttt gggatgtgtg
    11041 gggcaatact ttgtacgact gcacgttcac caactgtgtc agctactact tccacatcaa
    11101 atgtcttcag ataacgaata ccgccatgaa caagtttggt cgagcgagag gaagtccctt
    11161 ctgcaaaatc ctgcatttcg atcaatcctg ttttgattcc tgatgctgct gactggactg
    11221 ccacaccggc accagtgatt ccgccaccga tgatcaacag atccaattct tcttgctgca
    11281 ttttttgaat gctatcttgt cttgtcttgt ttgaaaacat atggatttcc tccttattca
    11341 aatgcttggg tagcttttac tgcttttttc caaccgttgt aaagtttttc tcggcgctct
    11401 tcctccattt gaacttcaaa tagcttgcca gcttcataaa attcgcggat ctcgtctgta
    11461 tctttccaat aaccgacagc taatcctgct aaaaatgctg cccctagcgc cgttgtttcc
    11521 aagttttctg ctcgttggat cggtacattc aagatatctg tttggaattg catcagatat
    11581 tcattgtttg ctgcaccgcc atctactttc aatacaggaa tcttgattcc agtgtcatct
    11641 tgcatcgtat cgatgatatc tctcacttga taagcaatcg actgtaaagt agctttaatg
    11701 atatcttctt ttgtcgtacc acgtgtcaag ccaaacatag cccctcgtgc tgcctgatcc
    11761 caataaggag cacctagccc aacgaatgca ggaacaacat aaacctcatc ttcgcttgta
    11821 gagcgtttcg ctgcgtcttc tgaatcgcta gctttttgca acatttgaag tccatctctc
    11881 agccattgaa tcgaagaacc agccacaaaa atacttcctt ctaaagcata gtaaaccttt
    11941 ccatttatgc cgtaaccaat tgtcgtcaat aaattatttt tggataattg cggtttttca
    12001 ccggtattca tgacaataaa tgaacctgta ccataagtat tcttgaccat gcctggttcg
    12061 aatgccattt gtccgaataa tgcggcttgc tggtctcctg ccatccctga aattggtgct
    12121 tcgcttccat aaaaatgata accttgcgta taaccataaa cttctgagtt agaagtgact
    12181 tttggtaaca tcactcgagg aatatttagt aaatctaaaa tctcctgatc ccaatctagc
    12241 tgatggatat tgaacaacat cgtccgactg gcattcgagt aatcagtaac atgtgcttgt
    12301 ccatcggtca gtttccatac taaccaagaa tcgatcgttc caaacatcaa ttcgcctttt
    12361 tcagctcgtt cttgcgcgcc ttctacatga tcaagaatcc agcgtatctt cgttgctgag
    12421 aaataggcat cgatcactaa ccctgttttt tcgtggaaaa attcttgata gccttcattt
    12481 tttaagttat ctgcaattcc agctgactgt ctggattgcc aaacaactgc atgatagatt
    12541 ggacgtcctg tatctttttc ccatattact gttgtttcac gctggttcgt tatgccaatc
    12601 ccagcaatct gatttggttt gatccctgat tcgatgaacg ctccggcaat aactgactgt
    12661 acagaattcc agatttcatt tgcatcatgt tccacccaac tttcttgcgg gaaatattgc
    12721 gtgaactctt tttgagaact gccgatgtgt cttcctttct tgtcaaagat aattgctcgt
    12781 gagctagttg taccttggtc aatcgccatg atatacgttg attctgtcat tgtgattccc
    12841 tccattatat gcaaacgctt tctttaacta ctcttatcat actactgttt taaaaaaaga
    12901 aactgtcaaa attttattct ttttttcttt ttttgacagt tttttattat gaagctattt
    12961 tttaatcaat tcttttcttt tttcagcgtc tctattcttc ttttcaatcg aataatgtcg
    13021 taaggtgacg caaattctga cagataatat tcacactgtt ttttgtttct ctggcaaaat
    13081 gtgatcacca aatcatattc tttaccttct tcataacttc caatctcaat accgccgatt
    13141 cccctcagtt ctctgatcag atattgttga aaagcaatac gataaatcgg cagactttct
    13201 aaatcgattc caatcactac tggttttgca atataaaaat cgatcatcaa aagaacattg
    13261 gcatactgga tcatcaggta actaagctca ctatcttcag ctcttttcct cggcaattgc
    13321 tgcaccgctg tttcctgtaa tttcgccagt aactgtgtta aggaatgccc gagcattttc
    13381 tgctgctgtt ccaaaagccg atcacgctca tagacttcga tctttccaac aaagaaaaac
    13441 cattcattgt tgatctggga aatttggtag cctactgcct tttcttcttt gattttcaaa
    13501 cgacgaggac gataatgcca taacatcgtt tcccgaatgt aagtatctaa aacagcggtt
    13561 ggtaactttt tgctccgtgt caaatcatac tgataaaata tttcttcgtc tactatcgaa
    13621 aaagaaacaa aaaagctgta aaacatcatt gtttcgtata cattcagttc tgctgctgtt
    13681 cgactaagat gcatcacgat gattggatcg attgcttgat aaagccggtc cgactgatag
    13741 agtaatttct tttctttcaa agttgcgtac gtactttttt cattcaatag tctttttttc
    13801 gtaatcccga gccagcaagc aatctgtgct tctgctgatg ccgtaaacgt tgtttctaat
    13861 acacggtcca atcctagaat gaacggtctt ttttctggtg tattttgcaa aaaaagcggt
    13921 cgctgatctt ccgggatata ttggaatagt tcataataaa aatatcgtat ctgtagctct
    13981 tctccgtata attgaccgtt tttgatttgt aatccaaatt cctctaagag ctggttcaat
    14041 tctttgatct ttcgaaaaac agaagactca ctgatattaa acgctgtcgc taatcgaacg
    14101 atggatactt gctgatgttc taataaatag acgagcatct taaacttcaa gctttcctgc
    14161 aataaaaaca atagaatttt ttccaaactt acatcttcgt ccaacacaag cagcgcctga
    14221 tactcatttc gctgtacttc tacttttttt tccatcattc ggccaatcaa ttcaatatct
    14281 tccaaatatt gatcaaacga ggcttttgac agttggacat gctctctcaa accattgata
    14341 gaggctgttc ccccttgcag aacaaggttt cgcaaaatca ggatctgtaa ctgctctttt
    14401 ctttctaata atgcttcaat cttcataggc agtattcgcc acctttgacc acaccatatc
    14461 tggcacacgg tattctggct ggaaaaatag ctcatgttct tctaactgtt caaacgtcca
    14521 tccttcttct actgcaattg tcagttggtc aagaaggtgg aacatctctt ttttgctttg
    14581 gagctgtgct ccaagaatct ttttttcaga atcaaataga aatttgagtt taactttgct
    14641 ttgatttatt gctgtaagct tgtagtctac gtatcctgtt tcaatttttt ggggataaaa
    14701 aatgctctct tcttctgtca aacctacgct tcctaagaaa tagccaaacc actgattgcc
    14761 gactggacga taggttccct gatcctttgt ctgtgagcca ctaattgtct tgctgacatt
    14821 tcgtgctgta cggacagcgt tatttactag agaaacatac attttttcat ttgttggacg
    14881 aaaagttact tggatcgcat cgccaatcgc ataaacgtct ttttgagacg tttgcaagta
    14941 gtcatctacc cagatcgttc catcatcatt caatgttaat tgttcttccc acatctgatt
    15001 gtcgggacga gaatgattag ccagcaaaac aagatcatag ttctcctttc ttttttccgt
    15061 ctctacaata gcggtttcat tttcggtcaa agacacaact tgttcttcta aaaaaagtga
    15121 aagcccttga tttttcattt cctttactaa cggttctgtc atttcaggat ctagataacg
    15181 aaaaagaatt gactttcggc tttcgtaaag atggatttgt ttttttcttg atgccaaccc
    15241 ttcagctatt tcgattcctg cttgtcctgc accaataatt gcaattttct ttgcattcat
    15301 gatcttttgc tgaagagccg ttacgttttg tatcttttta actgggtgaa taagcgttga
    15361 ttctgcagaa atatttcgaa acttttgatt tgaaccagtc gctagaatca aacgatcaaa
    15421 atccaatttt tttcctcctg ctaaaacgac ttttttttct ttcaatccta ttactgtctt
    15481 ttctgtgtaa acatggattc gatagcggtc agtcagttct tctttcgtga tccaagaaag
    15541 ttgtttttca ctggtaacct ttccttttaa tatcaaattg aacccgctgg gaatccatcc
    15601 taacttcgat tgcttttcaa tcaaatatat ttcacttgtt ggcatcaatt ggcgaagata
    15661 aatagccgca tgaatccctg caaacgaccc gccgacaatt acaatgttca tctgcattcc
    15721 tctttcttat aaggtgttct actgctaact tctcaatcag aaaacacagt attcataact
    15781 cgctttcaac cttatgatac atagcgatgc agcagaatca accctctcca tcataccgtg
    15841 ttctaggtct gcttctcaat aatgtactag tggacctaat tgaagagtat catttctgaa
    15901 gagatgtttc aaaaaatgta ctcttataaa agaaaaaaac tcgcctttta cgtacctctg
    15961 tccaaatagg aaacagaaaa gaacgaaaag acgagtttta gcgtgtgtaa tgtaatagtt
    16021 agaatagaca catcatttat ttttaattct ttaatgcttc aacaactgct tcgcctaatt
    16081 gatgagcaga ttgaggattt tgccctgtaa gaagccgccc atcaatttga accacttctg
    16141 aaaaagctgc tccagactga tagtttgctc ctttttcttt caaagcatct tctgctaaga
    16201 atggtacttc attcgttgta ccatttgctt cctcttcttc attactgaac ccagtcactg
    16261 tacgtccagc taaaatagat tttccagact catctgtaac tggcaacaat cctacaacac
    16321 cgtgacaaac agctgagacc agcccttcat ttcgatagat ttgtgaagcg actttcccaa
    16381 gatctattgc ttctggaaaa tcccacattg ttccgtggcc accagcataa taaaccgctg
    16441 cataatcatt tgcatccaca tcatcaggac gtaaggtatt tcccaaggcc cgatttctga
    16501 aatcttcatc ttcataatag gcccaatcga tttcatccat attcaaaaca ctttgcggtt
    16561 ctaaaggtac atatccgcct ttaggactga caaaatctgc atcgatccca ttttttacta
    16621 atacatcata aaaatgagtc agttcactta accacaaacc tgtaggacgc tcgatctttt
    16681 cgtacttttc tacatttgtt aatacgatca atactttcat tttcccatcc tctttctata
    16741 aaaattgcgt tattgataac gtgctttcaa gaaattatat aatgggcttg gcaaataaag
    16801 ctctttatat gcttcttcaa aaggtttcgt tacaaattca tcctcacggt tttccaataa
    16861 tttctctgcc cagtcaggat cgacgatcat ttgctgtcca attgctgcaa gttctgcata
    16921 tttcaatacc tgctctacat cttcgcgatt gcggatccca ccgacaccaa ctaaagggat
    16981 ctttccttga atcgtgttat ggaccacttc aagaatcgat ttatcgttgt atttatcaga
    17041 acgagctatt cgatcataca tatttaatga aacatgcaga taatctagtt ttgactcacg
    17101 taatcgttca agaagccaga tcgtgtcttc aaaacggata cctggtgttt catattcttc
    17161 tggtgagaaa cgatacccta cgatgaatgg ttttttagca tattgttcaa ccgctgagaa
    17221 aaccgttgtt aataattctt caataaaacg ataacgattt tccaagctac cgccccattg
    17281 atcctctcgt ctattcgaat gcggagagaa aaattgttgg atcaaatacg tatttgctcc
    17341 atgcaactcg attccatcaa atcctgattc aatcgctcta cgtgtggctt tgccaaatgc
    17401 ttggattgtt tcaacgattt cttcttcact cattgctcgt ggtgtttccg catcaggacg
    17461 ttttgctgca actgcacttg cagatacagg ctgttctcca gataaagttg ctcgacttgt
    17521 cattcgtcca ccgtggaaaa tctgaataat cgcttttgcg ccttctcctt ggattgcttt
    17581 cgctagctca cttaatcgag gaatcatttc atcatgagcg atgctgagtt cacctggcca
    17641 gccttttccg atatcttgga catttgcagc accagtaata acagcaccaa gacctttaga
    17701 acgttgttta taataagtga tctcatcttc ggtgatcact ccattgaaaa agctgagttg
    17761 tgtcgtcata ggcgacagca tcaatcggtt cttcaattga actcctgaag gtaaagtaat
    17821 tgattgatca aattgtgaca ttgtcattcc tcccataatt cgctctgatt aacctatcga
    17881 gaatcatcta tcattaaacc actcgattag ttgtacatgc aactaatttg cttaaaaaat
    17941 tttatttttt ttgtaactta tctgttgcat cattcattgt ctgggctaat tctttacttg
    18001 cttcatttcc caaaactgct ttgtatgact gatgaagttc ttcccagcta ccatcgactt
    18061 ccttagcaaa ttggcgtcct tgttctgtaa gataaactaa tgacaagcga ccttcgctaa
    18121 ctgttttgac gtatccttta tattctaatt tttcaacaaa acgagcaatc gttgatgggg
    18181 cggaatagag catttgtgca atatcttttt gtggtattcc atctttttct tttaatatca
    18241 ataaaataaa accataagtt ggtgccagac ctgtccttct aaacgcttca tcagctaatt
    18301 tattcaacat gcgatctagc tttttcacgc taaagaaaag acattcatct aacgcttttt
    18361 caattggctt ttccatttca tcccctccat tggcgtacat atcatagtat gatttttatt
    18421 ttttgtcaaa aaacacggaa taatgtccgc ctcctctgtc tagatttgaa aaaaagaagt
    18481 agccatcttc aaagaatttc ggcttgtttt cgacaaaact ccttctggaa caccgtgaat
    18541 cagaggttgt gaaaaagtcg tttagttttg agtatgaaga aaaattttgt gaaaatggct
    18601 ttccacattt ttgcataaat ttggcttaat accgaaaaac tggcttttga acaccgttta
    18661 tatcagaggt tgtgaaaaag ccgtcctgca tcaagtaata agaacagcca aacaaaaata
    18721 gttcctcata ttttgtcgtt ttttgagcta atgccgatgg tcagctgatt gtacaccgtt
    18781 tattgagagg tcgtgagcct gccgttgaac tccgagtcac aaggagcaat ttcaaaaaac
    18841 agcttctcat gttttatgac tatggtgact tgtgatcgag gagttggcag gagaatactg
    18901 tttattcgta aagaaggggc tgtgacaaga cttttgtcac agtcccttat ttccttttat
    18961 tataggattt cgccatttga ttggatgact tgtctgtacc aatcaaatga ttttttcttt
    19021 aaccgtttct ttgtgccatt cccttcgtca tcttgatcaa catagatata tccataacgt
    19081 tttgacattt gtgaagtaga agcgctgacg atatcaatgc agccccacga cgtataacca
    19141 aataaatcta ctccatcttt gatagcttcc ttcatctgct caatatgctg cttcaaatag
    19201 tcaatgcggt aatcgtcttg aacggttcca tcttcttcta ctttgtctat agctccgagt
    19261 ccgttttcag ctatgaacag tggtttttga tacctatcat ataattgatt caaagcaatt
    19321 ctcaaaccct taggatcaat ctgccagccc cattcacttg atggaagata cggattttta
    19381 attcctccag ttattaagtt tccttggacc ccttcccaat tttctggatg agcactgact
    19441 gcactagaca tgtagtagct aaaagaaaca aaatcaacag ggtaggctgc taagatttct
    19501 tcatcaccta cttctatttt taagttgaca cctttttctt caaacattcg ttgcgtatac
    19561 gctggataat atcctcttgc ttgaacatca ctaaagaaca aagaagttct ttgctgctca
    19621 attgtttttt gaatatcatc cggatcacaa gaatacggaa caagaagctg atagctgatc
    19681 atacaaccga cttgtgaacc tttgataatt tcatgacaag cttttgtagc taaagcactt
    19741 gctataaact gatggtgtgc accttggtat ttcgcttgtt ccaaatgggg atggttttct
    19801 tcaattactc ctacgctaac aaaagggtga tgttttacac agttgatctc attgaaagtc
    19861 atccaatatt ttactttatt tttatagcgt ttgaatactg tttcagcgaa acgaacgtaa
    19921 aactgaataa gctttcgatt aggccatcca ccatattctg taaccaaata taacggcatt
    19981 tcataatggg aaagagaaac gactggctca atcccatatt ttagacattc gtcaaacact
    20041 tgatcataaa aaaataatcc cgcttcattg ggtgtttctt catcaccatg aggaaaaatt
    20101 cttgtccatg cgatagacat ccgatagcat ttaaaaccca tttcagcaaa taaacggatg
    20161 tcttctttaa agtgatgata gaaatcattt ccgtgtcttt taggataata atacttgctg
    20221 ttgggatcct gcgccgcttt tacttgctga tgcgtcatca tatgccattg atctgtccat
    20281 ttttctttag gtaaatcgct gtcgaatgta tacacatcag acacagataa tcctttttca
    20341 tctacatccc atgcaccttc acattgattg gccgctgtcg ctccgcccca taaaaatcct
    20401 tctggaaatt ttttcatatc gatcccccta aacttgctag ttttcttgtt tgattatttt
    20461 tcttatgact agtaatgata tttttgaatc tactactatt tgtaacaata accattgtgg
    20521 tcggatcata tccctctttg attaattctt gcaaatctac ttccatcaat aaatcgcctt
    20581 tacgtacttt ttttccatca gatactgcac tttgaaagta tttgccattt aaattcactg
    20641 tatcaatacc gatatggatc agtatctctg ctccttcttc actgactacc ccaattgcat
    20701 gtttcgttgg aaaaactgtt gaaacttccc cgtttacaga actgtagatt tttccgtccg
    20761 ttggaatgat cgcaaaccct tctcctaaag atccactagc aaatgttgga tcgtttactt
    20821 cagataaatc aattatttca cctgctgcag gagtacttaa cactatatcc tttgaggatt
    20881 cttcttcgat ttcatccttg tagccgaaaa gaaaagttag catacaggaa ctgatcatcg
    20941 ctaagaaata actagcaact agccaaacaa atccttcacc aatataaatc gggaaagaca
    21001 aaatacttct tgtagcaact gccaaagcac cggctcctgc ggcaccagct actccaccaa
    21061 atatagctcc acatatacaa gcaatgtaaa atggtcgttt atatttaaga gtcacaccat
    21121 aaacagcagg ttcagtgatt ccaaataatg ctgtgataaa tgcagataaa gataaacttt
    21181 ttactttctt atttttcgtt ttcaaaaata ccccaagtac tgctcctgct tgcgcagcta
    21241 cggcagtcat acatgctgga cctagcgtat cacgaccgta gaaagaaaga ttgttggtca
    21301 ttactggtac aagtccccaa tgtaacccaa aaatcactaa cacttgccaa aatccgccga
    21361 taacagcacc agataaaaca gggctaaagt tataaacagc taaatatcca gctgcaatca
    21421 gctgacttgc ataatctgca attggaccta caactaataa aataattggg accatcacga
    21481 tgatacaaat gaatggtgtc aataaatttc gaatactttc gtgcaagctt ttgcgaatct
    21541 taggctcaat ataagaaaga acccatacag ctaaaattgc tggaataacc gtacttgtat
    21601 accttgctag aatcacgggc atccctagaa atcgcaaggt tatgctatca ctatatgcac
    21661 tgacaatcgt aggataaacg agagctgctg caataaccat agcgatgaaa cgatctgtat
    21721 tgaacttttt tgcagcagta tatgccaaaa atataggcaa gaaatagaaa aatgcatcag
    21781 ctgctgcatt caaaatgata taagttcctg attcagtagt taataaattc aaactcgtac
    21841 ataaggcgag tactcctttg atcaaaccac ttcctgccaa gacgcctaaa gtcggggcaa
    21901 aaacagcggc gatcatgtct ataaaagagt ttaaaatgcc ttttttctca gttcctctct
    21961 ttccactaga tgcatcatct tctagtttca tttgtttgga taatacgtca tacacttcat
    22021 ttactgcatt tcctactacg acttgaaaca ttcctccagc ttcgactaca gtgatcacac
    22081 cttctagttg ctctaccttc tccttatctg ccttcttatt atcttttagg gtaaagcgta
    22141 accttgttgc acaatggtaa acattgctga cattttcttc gcctccgaca aatcggacaa
    22201 tatctttcgc taattgtgaa tagtccattt ttacttcccc cttgttaatt aatgccgtag
    22261 caatccttct tacaagataa gtatagttct tagaaaacgg tttctccatt caattagtac
    22321 cccttctaat agcgaacgaa actgtacaat agtgtgtatg gtttacttag ccgagcaaat
    22381 cactcactgc ttgaaagaaa acaagtatat cgtttctctt atagtcaaag gagacaaaaa
    22441 aaacacgctt gatcttcctt tgctgatgta taagtggtat ctcaagctga tactctgtga
    22501 tttttgtttc tacttggctt ttgataagac agcacaacaa agtctcggca tttaacggaa
    22561 gcataactag gtcagaacta ggagagatta gttgctgaat ggaatctatc tttttatgga
    22621 tttttatttg attttcttct ttgtagtaat gatcaagcag ttttgttttc tttttttctt
    22681 caactatggt cagcttttca cgcaaaatct gataaatctc ccaagattca aactcatcta
    22741 gaacactagg tagataaatc aaatcatccc actcaaaaat aagctcttgc tcagtagtca
    22801 aaattgcatc atattccgtt attttgtttg attgattttt gaaaagatac actggttcta
    22861 tccggcattc tttgatccac atgttcatac tttgttgaat ctgttttttt agtgtattca
    22921 aaatactaat tggctggtta ctaacaatca gtatctcccc ttgttttcga aaaggattca
    22981 acgcttcggc aagatacaaa acaaaataag cgctttcagc taaattggga cgtaattgaa
    23041 aatgtattgg gaagaaacgc cgaattaaat aaatttctaa aggataacgg gagatgatct
    23101 tttgtacaaa atgattcatg gtattatgtc cggcatccat cctcgttttc atttgtttga
    23161 tatgggttag caaaaattct gtttcatgga acaataacga aacaggaaga tctaaaaaac
    23221 gaataatcgt ttgctcaaat gctcttagat tcttctctat ttctttgttt ttttcatcat
    23281 taatcatcaa ataatttaac tctaatagtc gattttcaat tgcttgtttt tcttgattgg
    23341 aaaaagaata gggtagttct gatgataact tttttgtgag ggtctctacc atcggaataa
    23401 ataatgtata cctgtttgtt ttttctaaaa gttcttctct tcgaagaatg gtcaaaacga
    23461 gataccgaga aaatcgtatg aagtcctctc ctgaaacaat ataattatat gatataagaa
    23521 cctcttttaa tgtcttttgg acagtcggca tgatattgga aaaaattgta ctgatttgtt
    23581 gaggaaacct tgcgtgttgg atcaccgttt ctgttccaac aagagataag aaaattcttt
    23641 ttgaattttc ttcagcaatg atttttagtc cttttgtcga cgaaacttcc agttctaatt
    23701 taggattgcg ctcgatccaa cgcaaaatcg tttttatttg taatgatacg gctgtttttg
    23761 aaagaaagaa aatgtctgct atcttttgca tactgacata ccctttttga aacaataagt
    23821 acgtcacaat agttgaagct cggaaatcca tgctggaata gattttatgt ttcgtcacct
    23881 cttgtatgat ttgctgttta gtttcagatt caattgattc taaaaaataa cctgaacgtt
    23941 tatttgactg gataatgatt ttcttctcgt agatagcaag catatttttg atttcagttt
    24001 ggatcgtttt cttgttggta cctaaagctt cagctaattc ttcgcttgtt atccaatgat
    24061 tcttatttct taatactaca agaatttgga gttgtcgttt atttaagtcc atttgcttac
    24121 acctcctctt tgttataaac atacaggaaa aacctctcaa atgacaatag tcacttgcag
    24181 aggtttaaaa atcatttgaa gaaagatcta tattcaaggt ttttaccatt agactggata
    24241 atttgtttat aatattcaaa actatctttt ttcaatctcc taaagcttcc gctcccatca
    24301 ttttgtctat ccacataaat aaagccatat cgcttcctca tttcccctgt tcctgccgat
    24361 acgatatcga ttggtcccca ccataagtaa ccaatgattt ggcaaccatc ttttattgcc
    24421 tcagcaattt cctgaagatg tatttttaga tactcacaac gttttggatc gtggattcga
    24481 ttcaattcat ctggtgcttc atctagtcca atgccatttt caactatgaa taatggtaat
    24541 tcataacggt ctgtcaattc attcatgaca taacgaaacc ctagaggatc aattggccaa
    24601 ctccaaggtt cgggagattt ttccttcaaa aagggattat cgaaaccatt tgctccgcca
    24661 gtatctacgc gcatttctgc ttctttttta tagacagcgc tacgataata actaaatgca
    24721 atataatctg ccgtgttagc ttgcaagcac tgttcgtctc catctaacat tactggtgca
    24781 cagtcttctt ccttccatgt cctgtaaata taagctggat aatgccctcg gcacatgaca
    24841 tcggagaaaa accatgaatt gcgtttaaat tgcagtgaac caaatacatt ctctggatca
    24901 cagtcatatg cataagtcgc taatgcagaa aagctacaca taatacccat cttagcattt
    24961 ggatcgattt tctttaataa ttgagtagca cgggcgttcg ctagaaacat atgatgacag
    25021 gcttgataaa tcactttttt tgttagtgta gcaatgggtt caagtttatc tctagcaggg
    25081 aatgtaacca atccagcagc tgcataaggc atacgaaaag cattattgat ctcattaaag
    25141 gtcatccaat atttcacttt cccttggtag cgttcaaaga cggtctgcgc ataaaattca
    25201 aaaaagtcga ttaattttcg attttgccac cccccatact ctcctaccaa tgctaagggg
    25261 gtttcataat gactaagagt aattactggc tcgatatcat atcgatttaa tgtattgatt
    25321 agtctgtcat aaaatacgag accagcctca tttggcttct gttcgtctcc tcttggaaat
    25381 attcgtgccc atgagatgct tgttcgaaaa gcttttaaac ctaactcctt cagtaaacga
    25441 atatcctctt caaatcgatg ataaaaatca atcgcttcat gagaaagata atggagtgat
    25501 tcgtctaaag caatctccca aatctttttt tcttcattcc actgaatact aggatgctgg
    25561 ttcatgatcc cgataagcgt atctgtcaca ttaggtaatt tgccgtcttc caaccatgca
    25621 ccttcacatt gattagcagc tactgctccg ccccataaaa aattttctgg aaatatttcc
    25681 tgatccataa ctatctctcc tcgctatcaa caatttctta cttgaagtat agctcgattc
    25741 atcaaaacaa gatattctaa ctatttcggc agaagagaag aaaaaaattg aatgctactc
    25801 ttcattttta atatttatat ataacaaata cataagacga tccccgaaag aaaacactcg
    25861 ttaaccatct aagcaaacga tttattttac ttccaacaca aatgcttcat aaggtcgtaa
    25921 atcaactttg ccgtctggta actgtttcag aggataattt gacacaataa cccttaaagc
    25981 ttcacgtgga atagtgaacg tctgcttttc actggacata ttacaaataa cgagccattg
    26041 ctggtcttca tatatgcgag aataggcaaa gacatgctca ggcgtctttt ccactaattc
    26101 atagtctccc cagacgatga tgtcatgttt tctacgcaaa tcgatcaatt tcttgtacat
    26161 ataaaaaaca gaatcttgat ctgctaacgc ttcttctgca ttgatttctt tataatttgg
    26221 attgactcga agccatggaa ttccagtagt aaacccagca tgttcacggg cgttccattg
    26281 cataggcgtc ctagcattgt ctcttccttt cgccttgatt gattccaaga tttcctcttt
    26341 tgtaaatcca gaagaaatcc tttcgttata catattgatt gtctcaatat cttttgcttc
    26401 cgcgatatca tcgattgggc aattcgtcat gcctaactct tctccttgat agatataagg
    26461 ggtgcctttc atcatatgga gcaagatagc aaacatcttg gcactttctt ttcgatacac
    26521 atcgtcattt ccccaacgag aaacaattcg cggaagatcg tgattattcc aaaataaact
    26581 attccatcct tcatcaccta aagatgtttg ccatttggat aatacttttt tcagttcaga
    26641 gatactcaag ggtttcaaat cccatttgtc tttgccttct tgctgatcta atcctatgtg
    26701 ttcgaattgg aagaccatgg ataattcatt tcttttagga ttggaataaa gtttagcgat
    26761 ttcggtagtt gctccccatg tttcgccaac agtcatcaga tttttgtcac caaaagttgc
    26821 ttgattcatt tcctgaagat actcatgcag tttaggtcca ttacttgtga tttctttatc
    26881 cggttctttg ccaatcatat cgatcacgtc catacgaaag cccccaatac ctttatcgat
    26941 ccagaaattc atcatttgat aaatctcttg gcgaacctgt ttatttttcc aattcaaatc
    27001 aggttgacgc ttgctataca agtgcagaaa atattgcccc gttttttcat ccaactgcca
    27061 agcagaacca ctgaatattg agcgcaatct attaggctct tttccattga ccggatcacg
    27121 ccaaatataa tagtcccgat aaggattttc tttattttta cgggcctcaa tgaaccacgg
    27181 atgttcatca gaagtatgat tcaccaccag atccatgatg atacggatat ttcttttctt
    27241 tgcttcttca atcagttctt ccatttcttc catcgtaccg aattctgcca tgattgcttg
    27301 ataattactg atatcatacc cattgtcatc attcggagac tcgtatactg gagaaagcca
    27361 aattgcatca atacctaaat acgccagata atccaacctg gaaataatcc cccgcaaatc
    27421 tccaatacca tcttgatttg aatcttgaaa acttcttgga tagatttgat aaacaactga
    27481 atggtgccac catttttgtt ccatgatttt cctcctataa atggatgttt ctattttttg
    27541 cttaatcatt tattttagtc tctctcacta aaaattgtaa cgctttcaaa tagctattgc
    27601 accttgttat cggtaacaga ataaaacaaa aaaacagctg aggaaattcc ctcaactgtt
    27661 ttttgaaagg attgatttat gcagggaaga atgctgattt cacaacttct ttataaacgg
    27721 tgttcactca gctgtccctt ggtattagct caaaaaacgg caaaatataa gaaactattt
    27781 tttcgttttt ttctctaata ctcaggccag aacgctgatt tcacaacctc tttatttcac
    27841 attcaatatc cacatgaatc ccacaaatac aaagaacaga ccatacatga ttggagatac
    27901 ttcttttcct cttttagctg ctaacatggt aattggatag aagataaaac ctaatgcaat
    27961 cccatctgaa atactataag tcaaaggcat gccgatcaag atcaagaatg aagggatcgc
    28021 aatctctaag tttccccatt cgatttgtcg caaagattgt gccatcaata ctcctaccat
    28081 aatcaatgct ggtgcagtta cttgtgaagt aacaactgat agtaatggag aaaagaacaa
    28141 gcctaacaag aagaaaatcc ctgtagtaat agctgtgata cctgaacgtc ctccaacggc
    28201 gataccagct gatgattcta caaatgcacc aacaggagaa gtaccaaata aggaacccgc
    28261 aagcatagct gttgaatcag aagctaaagc tttccctaca cgaggcatct tgttgtcttt
    28321 catgaaacct gcttggtttg ctaatccaac taaagttcct gctgtatcaa agaaagtcac
    28381 aagcaagaat gtcagcacta cgacccacat ttgaagagaa ttgatgtccc ctacatgttt
    28441 taatgcaact aaaaatgttg gtttcaaact tggtgctgcg gcaacaattt cagaaggcat
    28501 tggaatcaaa cctgtgacca gacctaaaat cgttgtagct gccatcccga tgaagatgcc
    28561 acctggtaca cgccgaacaa gcaagatagc agttacgact aatccaaaaa tcgtcaacca
    28621 agtagaacca acgttgagtg atcctaatcc tactaatgta gattcattcg aaacgatgat
    28681 cccaccttcg ctcagaccaa ggaaagcgat aaatagaccg ataccgccag aaatcgcata
    28741 tttcaaatct gctgggatcg catcaatgat catttcccgc agcttgaaga ttgtgatcaa
    28801 aataaaaatc aaagaagcaa cgaatacgcc ggctaatgct gtttcccatg gaatccccat
    28861 tcctaaacaa acagaataag caaaaaaagc attgatccca agagcaggtg ccgttccaat
    28921 tggataacgg gctaataccc ccattaaaac acagccgatc gcactggcta atgcagtcgc
    28981 agtaaacact gctccttcat ccattccaga agcacctaat acagttggat ttacaaataa
    29041 gatataagcc attgagataa atgttgtaaa tcctgccagt acctcacgtt ttaccgtggt
    29101 gttcaatttt tctatttcga agtaggaaag tattttttcc ttcatcttcc atccccctaa
    29161 atccgaacat tataaataaa gtccgttaaa attt
//
LOCUS       DAXWOO010000015        29108 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.015, whole genome shotgun sequence.
ACCESSION   DAXWOO010000015 DAXWOO010000000
VERSION     DAXWOO010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29108
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.015"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(<1..1031)
                     /locus_tag="ACGE70_000545"
     CDS             complement(<1..1031)
                     /locus_tag="ACGE70_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFF8692206.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1113..1802)
                     /locus_tag="ACGE70_000546"
     CDS             complement(1113..1802)
                     /locus_tag="ACGE70_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFF8692207.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="ACGE70_000547"
     CDS             complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="ACGE70_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFF8692208.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2329..2832)
                     /gene="rplJ"
                     /locus_tag="ACGE70_000548"
     CDS             complement(2329..2832)
                     /gene="rplJ"
                     /locus_tag="ACGE70_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFF8692209.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3036..3163)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="ACGE70_000549"
     CDS             complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="ACGE70_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFF8692210.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="ACGE70_000550"
     CDS             complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="ACGE70_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFF8692211.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4800..5468
                     /gene="sdaAB"
                     /locus_tag="ACGE70_000551"
     CDS             4800..5468
                     /gene="sdaAB"
                     /locus_tag="ACGE70_000551"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFF8692212.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5481..6353
                     /gene="sdaAA"
                     /locus_tag="ACGE70_000552"
     CDS             5481..6353
                     /gene="sdaAA"
                     /locus_tag="ACGE70_000552"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFF8692213.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6444..7544)
                     /locus_tag="ACGE70_000553"
                     /pseudo
     CDS             complement(6444..7544)
                     /locus_tag="ACGE70_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(7636..8523)
                     /locus_tag="ACGE70_000554"
     CDS             complement(7636..8523)
                     /locus_tag="ACGE70_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFF8692214.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="ACGE70_000555"
     CDS             complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="ACGE70_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFF8692215.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9246..9416)
                     /gene="secE"
                     /locus_tag="ACGE70_000556"
     CDS             complement(9246..9416)
                     /gene="secE"
                     /locus_tag="ACGE70_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFF8692216.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="ACGE70_000557"
     CDS             complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="ACGE70_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFF8692217.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9754..10398
                     /gene="cbpA"
                     /locus_tag="ACGE70_000558"
     CDS             9754..10398
                     /gene="cbpA"
                     /locus_tag="ACGE70_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFF8692218.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10454..10957)
                     /locus_tag="ACGE70_000559"
     CDS             complement(10454..10957)
                     /locus_tag="ACGE70_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFF8692219.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10970..11016)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11554..12519
                     /locus_tag="ACGE70_000560"
     CDS             11554..12519
                     /locus_tag="ACGE70_000560"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFF8692220.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12618..13790)
                     /locus_tag="ACGE70_000561"
     CDS             complement(12618..13790)
                     /locus_tag="ACGE70_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8692221.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13777..14922)
                     /locus_tag="ACGE70_000562"
     CDS             complement(13777..14922)
                     /locus_tag="ACGE70_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8692222.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14936..15868)
                     /locus_tag="ACGE70_000563"
     CDS             complement(14936..15868)
                     /locus_tag="ACGE70_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8692223.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15985..17064
                     /locus_tag="ACGE70_000564"
     CDS             15985..17064
                     /locus_tag="ACGE70_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFF8692224.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17061..17690
                     /locus_tag="ACGE70_000565"
     CDS             17061..17690
                     /locus_tag="ACGE70_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFF8692225.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(17800..18027)
                     /locus_tag="ACGE70_000566"
     CDS             complement(17800..18027)
                     /locus_tag="ACGE70_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFF8692226.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18130..20718)
                     /locus_tag="ACGE70_000567"
     CDS             complement(18130..20718)
                     /locus_tag="ACGE70_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFF8692227.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20738..21397)
                     /locus_tag="ACGE70_000568"
     CDS             complement(20738..21397)
                     /locus_tag="ACGE70_000568"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFF8692228.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21446..21949)
                     /gene="trmL"
                     /locus_tag="ACGE70_000569"
     CDS             complement(21446..21949)
                     /gene="trmL"
                     /locus_tag="ACGE70_000569"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFF8692229.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22170..23036)
                     /locus_tag="ACGE70_000570"
     CDS             complement(22170..23036)
                     /locus_tag="ACGE70_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFF8692230.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23145..23786
                     /locus_tag="ACGE70_000571"
     CDS             23145..23786
                     /locus_tag="ACGE70_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFF8692231.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23848..25209)
                     /gene="mgtE"
                     /locus_tag="ACGE70_000572"
     CDS             complement(23848..25209)
                     /gene="mgtE"
                     /locus_tag="ACGE70_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFF8692232.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25227..26126)
                     /locus_tag="ACGE70_000573"
     CDS             complement(25227..26126)
                     /locus_tag="ACGE70_000573"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFF8692233.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKSYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26128..26925)
                     /locus_tag="ACGE70_000574"
     CDS             complement(26128..26925)
                     /locus_tag="ACGE70_000574"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFF8692234.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26903..27607)
                     /locus_tag="ACGE70_000575"
     CDS             complement(26903..27607)
                     /locus_tag="ACGE70_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFF8692235.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27738..28313
                     /locus_tag="ACGE70_000576"
     CDS             27738..28313
                     /locus_tag="ACGE70_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFF8692236.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28431..29108
                     /locus_tag="ACGE70_000577"
     CDS             28431..29108
                     /locus_tag="ACGE70_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFF8692237.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8565 a         6168 c         5232 g         9143 t
ORIGIN      
        1 aatacagtat tatttcggtt gcttgctgct tcacctagcc cttgtactcg tgtttgattg
       61 gcgacttgac caaattgatt cccgacgtct aataaaataa tagctaaaag caaaatggcc
      121 aaatggcttc cgccaaccaa taaagcaata aagcttattg tttgcataac aagtcccata
      181 tacacgattt ttctttcaga ataagaatct gccagtcgtc ccacgatagg tgcagcaact
      241 gctccagaca aaccaaaaat cgctaatatc cctgcttcga atgttcccca attgtaggcg
      301 ggactgctga tataaaaaat caacgtagac caaaagatcg aaaatgttcc aaacatgaaa
      361 aatccactga ctgccgcttc tcttaataga cgttgagaag caactaaatg cggcaaactt
      421 ttaagtgaat caaggtatga aacagctgta ctactttgct ggacagaacg cggcattttc
      481 cagtaaagca gaagtgtaag aattccgact gcacataaag caaataaata gatcatccgc
      541 caagaaaaaa gactggcaac aataccagat accgtccgag aaagcaaaat ccccacgagt
      601 aaaccgctaa gcaatgtacc catgaccttc cctcttgctt ttggtcccgc tagtaccgca
      661 ccgtaaggaa tgatgatttg cggcacgatc gaaagtaatc ctattaaaaa agaagcgcat
      721 gcaaataacg taaacgaagg cgcaaaaaaa gcggccaaca gagagacggc agataacgct
      781 accattcgaa tgatcaattt tggacgatcc accacgtccc ctaaaggaac taaaaacagt
      841 aaccctaacg catagcctaa ttgggtcaac atcgtcaata tcccgactgc acttgtgcta
      901 acagataaac tagctgctac ttttgttcca atcggctgga tgtaatacat attggcaaca
      961 acaacgccac aagtgattga taatagtaaa ataagcgaag ttgttaaagg tgttttttca
     1021 ttactcttca ttgatttccc ccatttccta tcttagttta caacttatgg aaaagattgg
     1081 aaatagaaaa atacaactat tcgccacact tattatttgt gcttttgttt tccttgtttt
     1141 ttcttgcgat ctttataaat gaagacaact aaccctacta gcacagtaac acttgcaacc
     1201 aacgtgactg cagttttgtt atatccgtga aacagagctt ctccaccaca cgcatacaac
     1261 acagacgaag ggatgactcc aatcacgatc gacaatatga tttgctgtag aggcaatttc
     1321 atcgcatctg cagcaaaatt gaccatagaa gatggaataa ccggtatcat atagcctaac
     1381 attacaccta tttttggatg tttcatttgt cggattgctt gcacccaatg attcgtttcg
     1441 gttttcttat ctaaaaattt taagcgatgc ataaggaaaa ttgcagtgat gttaccaaga
     1501 gcattaccca aaatgttgat gatcgcacct gtcagtggtc caaagctcaa accagcaatg
     1561 actccaacta ctgaagtagg aatacctgga acagcacaca tcaccgctgt caatacgatc
     1621 aagagtatcg atgcaaaagt cccatgcgaa cgaatatgtg cgagtagctc tgccttactc
     1681 gctctcggat ttaaaaataa ttggatgtct gtctgatatt ctaaatacaa ccgatagccc
     1741 actaccagta tcagaatgat tcccaacgag atcaacatca atcggattat tttctttttc
     1801 atatttcctc ctgaaaaaaa tctatattca ttaaataata gtttaacata aaaacaaaaa
     1861 agcaccgaaa tccaacatag gatctcagtg cttttaaata gtaaaaatta tttaacagtt
     1921 acagatgcgc caacttcttc aagtttagct ttcaattctt cagcttcttc tttagatacg
     1981 ccttctttaa ctggtgcagg agcgccatca actacagctt tagcttcttt caagcctaaa
     2041 ccagttgctt cacgaactgc tttgataact ttaacttttt ggtctccagc tgcagttaat
     2101 tctacagtga attctgtttg ttcttcagca gcagcagcgc caccagcagc agctacagct
     2161 acaggagcag cagcagatac gccaaattct tcttcaatag ctttaactag gtcgttaagt
     2221 tctaggatag ttgcttcttt taactcagca acgatgtttt caatgttcaa tgccattata
     2281 atttcctccg tttaattgtt cttttttatt taatgcttta tgcggcaatt aagctgcatc
     2341 tccatcttct ttgtctgcca ctgctttgac agcgtaagcc acgttgcgga ctggcgcttg
     2401 tagtacagat agcaacatag atagaagtcc ttcgcgattt ggcaattttg ccaatgctgt
     2461 gatttcttcc aatgaagaga ctttaccttc gatgataccg cctttgattt ctaatgcttt
     2521 tgcttcttta gcaaattcgt ccatgatttt cgctggtgcg actacatctt cgttactgaa
     2581 tgcaacagct gtaggtccag tgaatacttc gtccatgcct tcaaggccag cttttttagc
     2641 tgcacgtgaa agaatagaat ttttgataac tttcatttct acgttagcat cacgaagttg
     2701 tttacgtaaa cgtgttactt catcaactgt taatccgcgg tagtcaacga ctactactga
     2761 tgcagctgct gtaaattttt cgcttacttc atcaacgatt gctgcttttt ttgcgataat
     2821 tgcttcactc atttttgagt ttcacctcct gaattttgat ggaagagttt tttgcactga
     2881 aaaggcaagt cacttttata gttactttgc tcaaccagta ttattcatca cctattcact
     2941 tcgttcatag tgattctact cgctttgctc gaaaggaaac tcctttcgct ttgctcaacc
     3001 agtattgttc atcatctctt cacttcgttc atagtgattc acaataaaaa actctatgcc
     3061 accgcagaca tagagggact attgaatata ttcaataaac gtcctcggta ggaaattaag
     3121 gcttgatgcc acctactgtc ttcggtacaa gttaattatt aacctcaact agcttagcat
     3181 gctaaactag ttgagtcaag aattattttt cagttggatc aatcaacatt ctaatattta
     3241 attagaaaga agcttggtca acatggatac caggtccaaa tgttgttgtg atcgttacat
     3301 ttttcatgta ttgaccttta gctgctgatg gtttagcttt tagcaataca tcatggattg
     3361 ctgcaaagtt ttcaactaat ttttcatcgt caaatgaaac ttttccgatt ggtacatgga
     3421 tatttccagc tttgtccaca cggtaagtta ctttaccagc tttcacttcg tttactgctt
     3481 ttgttacgtc cattgtaact gtaccagttt tagggtttgg cattaagcct tttggtccta
     3541 atactcgtcc tagacgacct acagtagcca tcatatcagg tgttgcaaca actacatcaa
     3601 agtcaaacca tccgttttgg attttttgaa ccatgtcgtc gtcacctacg aaatctgcac
     3661 cagctgcttc agcttctttt gctttgtctc ctttagcaaa aactaaaaca gtttgtgttt
     3721 taccagttcc gtttggtaat acaacagcac cacggatttg ttggtctgct tttttagggt
     3781 ctacgttcaa acggtatgca acttcaactg ttgcatcaaa ttttgcaatg tttgtttctt
     3841 ttgctaaagc tactgcttcg gcaacaggat aaactttact tgaatcaact ttttttaatg
     3901 catcttgcat ttttttgctc tttttagcca ttttggttcc tccttgattg tggttataac
     3961 ggttgtacct cccacgtgtt ctatatcttt caatatagag ccaactgaga agctgctgtc
     4021 ttagggtgta tagattattc tacagtgatc cccatgcttc gtgcagttcc ttcaaccatg
     4081 cgcattgctg cttcaacgtt agctgcgttt aggtcttcca tttttaattc agcgatttct
     4141 cttacttgat cgctagaaac tttcgcaact ttatttttgt ttggttcgcc tgaacctttt
     4201 tcgattttag ctgctttttt caatagaaca gcagctggcg gtgtttttgt aacgaatgtg
     4261 aatgaacggt cttcgtatac agaaataaca accggaatga tcaaacctgc ttgatcagct
     4321 gtacgagcgt tgaattcttt tgtgaatccc atgatattga tacccgcttg acctagtgca
     4381 ggacctactg gtggagctgg agttgcttta cctgcaggaa tttgcaattt aacgattttt
     4441 tctactttct ttgccacgag acatacctcc ttaagtccgt gatgtggtta attggagatg
     4501 catatttctc ctcccacgtt ttatctgatc tgaaaaaatc cagacaaata atgtgcaaaa
     4561 caatgcacac tggtacagta tagcaaattt atttgttttt tcaagatttt tttctgctat
     4621 ttttacattt tgcttgaata aacgctaaat ttattctaaa aaaacaacaa tttttcgttc
     4681 tcatttcttc ttttatgtta cacttgcgct agcatggttc aacagatgaa agcaaacaat
     4741 aacacggttt tttagaccct tgtacaaaga ttcctacata acaaaatgag gtggagaaaa
     4801 tgaaagattt aaggtataga agtgtctttg atattattgg acctgtgatg attggtccaa
     4861 gtagctctca cactgccgga gctgctagaa tcgggaaaat catacgtagt atatttggcg
     4921 aacagcctga ttctgtagat atttatctat atgaatcatt tgcaaaaacc tatcgggggc
     4981 acggaacaga tattgcatta gttggcggcc tgttagggat ggaacccgat gatgaacgtt
     5041 tagctgattc tttgaaattg gcatatgaag ccggtatgga agtcttgttt gttcctaaga
     5101 gtgaaaaagc cgaacatcca aactctgtaa agatgcttct aaaaaaagga acacgaaagc
     5161 tttcagtcac tggcatttcg atcggcggtg gcaatatcca gatttctgaa ttgaatggat
     5221 tcaaaatttc tttaagcatg ggtactccta ccttgatcgt tgtccaccaa gatgttccag
     5281 gtatgattgc taaagtgact agtatacttt ctgagaccaa tacgaatatc ggcactatga
     5341 cagtgacacg ggaatcaaaa ggtgaaaatg ccattatgat aattgaagta gatgatcctc
     5401 aagtagaaga aacggtaaaa agactcaaac agctgccaaa tatcgacagc gtaaattatt
     5461 tcgattaagg agctaaaaaa atgtttttat cgattgaaga attagtagat caagcaaaag
     5521 cttatccaag tgtagcagaa ttgatgattg cagttgaaat ggaaatgagt ggccgttccc
     5581 gtagtcacat catcgagatc atggaaaaaa acctctcagt aatggaacaa tcaatcaacg
     5641 agggaaaaga aggtgttacc tccgtcacag gaattaccgg tggtgatgca aaaaaattag
     5701 atgattattt aaatgaggca gactttttaa gtggagaaac aatcttaaaa gctgtacgca
     5761 atgctgtagc tgttaatgaa gtcaatgcaa aaatggggtt gatttgtgct acaccgactg
     5821 ccggaagcgc gggtgttgtt cctggtgtgc tgatggctgt ccgagatcgg cttgatttga
     5881 cacatgaaca gcaattggaa ttcttattta ccgctggcgc tttcgggtta gtcatcgcaa
     5941 acaacgcttc tatcagtggt gcagaagggg gatgtcaagc agaagttggt tctgcaagtg
     6001 caatggcagc agcagctttg gtttgtgcaa aaaatgggac tcctcatcaa gcagcacaag
     6061 cagtagccat tacattgaaa aatatgatgg gactgatttg cgatcccgtt gcaggcttag
     6121 ttgaagtccc gtgtgtcaaa cgtaatgctt taggctcttc tcaagctttt atctctgctg
     6181 atatggcatt agcgggtatc gaaagtgtca ttccgccgga tgaagtcgtt gctgcaatgt
     6241 accaagtcgg acgtcaaatg ccgtcgattt tcaaagaaac agcagaaggt ggcttagctg
     6301 ttacgccaac tgctaaacga cttgcggaag agattttaaa taagagggcg tgataaagct
     6361 ccttaatttc gagtattaag agattagtta tcttcataac aaaaaacgaa gagaggacta
     6421 attgcccttc tcttcgtttt ttattaatcc atcaatttcg tattttttac ttcatcgatc
     6481 gtttttgttc ctgcaagttg catcgtgatc gacaattctt tgttcaggtg ttcaaaaaca
     6541 gatgttactc cttctgcccc acctagattc aacccataaa tcactggtcg gccaatcgca
     6601 actaagtcag caccacttgc cagagctttg aagacatgct ctcctcgacg gacaccagag
     6661 tcgaaaatca ctggtacacg tttgttgact acttcggcaa tttttggcaa tacttcaaag
     6721 gacgcaggac ctccatctaa ttgacgtcca ccatgattgg atacccagat accatctgcc
     6781 cctgcagaga tagcgattac tgcatcttct ggcgattgta ttccttttac gataactgga
     6841 agatgggtga tttctttgat tgttttgatg tcatctggtg tcaagccttg ttttgcagct
     6901 gcataaattt ctgcgattcc ttttccttcg ccattaccac ttgcactttg ttcgctatac
     6961 gcagctaagt taggcattgg taatggaaat tggaattgat tgatgacgtc ttcttcacgg
     7021 taaccaccga gcgttgaatc tgccgttaaa atgatagctt tagcccctgc ttctactgct
     7081 ttgttcaaaa tgaactcatt gaatctgtcg tctttactca tatataattg gaaaaactgc
     7141 ggtgcgcctg gcgctgcatc ggctgcgtct ttgattgtcg tatttgcata agtgctgatg
     7201 gaaaagattg atccagcagc agctactcct ttagctgtat cagcttcccc tttttcatgt
     7261 gccaatcctt gggccgctga tggcgcttgg ataatcggtg tatctaattc aattccgaaa
     7321 acagatgtat gcaaatcggc tgagtcgatt ccgcgtaata tccgtggcat gattttcttt
     7381 gtattaaaag aagtcgtatt ttctttcatc gtccactcat cttcagaacc accgcgaata
     7441 tagccaaaag ctcctgcttc catgcgctct tttacacgac cttctagtta ggcaatgttt
     7501 acaatatcga ttggatgttc ttcattactt gtatgataag tcatagtaga tccttctttc
     7561 atcttcttga ttacatataa atcatactaa acttacaaaa agtaagcaaa catttactct
     7621 cagatacttt tatcgctatt ctttccaaat aaatcgactt acaagctgat ctacttcttg
     7681 gttttcatga agcatactat gagaggcatt ttcacctgtc actacttttt cctctatttc
     7741 atttccgcgt tgcttcaaca acgcatagac cgctaatgca ctgtttaacg gtactgttcc
     7801 atcactcaag tctgtttcat ctagttgacc agcaatcaag aaaaatcgag tggttatagg
     7861 aatattaccg atcatttgct ggtaatcttg atagcggtta gaaacgacag caggcccctt
     7921 tgctaactca tctgtcagag actgcgcact atcatcgaca aaatcattaa aaggcgcgcc
     7981 aattgctgtt aatgtctgaa cttgtggcaa tgacgtatcc tgtccatacg ttcctaaata
     8041 gcgtagtgca ctaactcctc ccatggaatg ccccactatg ttgactttat ctatgccgta
     8101 gttttctttc agatacaaaa gacaagtttt gatccattct gcttgattcc actcgttatt
     8161 tttattatct tcaaacaata cttggaccat cggattgtct gcttttccag acaaatctcc
     8221 tgttgcttgt atttgtccat ctgttcctac tgttaaaacc aattctttta ctgcttgctg
     8281 atttttttcc aaacgccgca acatcccttt aaaggaattt tctgtcccgc tgtagccgtg
     8341 gaaaaaaagt gttggaatag tggttttttc tatcacttta ctggattctg ccgttttttc
     8401 tttatcagaa acttcagtag tatttatctt cgtcgtctgg caaccactta acgcaaacaa
     8461 actgataagg actaaagctg ctgcagttgc acgtttgcat aaatttttga ttggttctgt
     8521 catacacctc ttcctttcca tatagaaaaa agaaagactt gaaaggccaa tcatatgatc
     8581 gacatttcaa gtctctttat taatcgattt tatctacttg atcgaaatca agttctgtac
     8641 tggtttcacg tccaaacata tcgatgttga ctttcaattt ttctttttct ccgtcaactt
     8701 ctgttacttg tccttctaat ccagcaaatg cgccttcgat gattttcacg acttcgccaa
     8761 cttctacttc tagatcattt tgtcgcaggc tcataccgat agaacgcaag atatgattga
     8821 cttcttctgg aagtaatggt gctggtttgc ttccggctcc gtgtgaacca acgaatcctg
     8881 tcacaccagg tgtgttgcgg acaacatacc atgaagcgtc tgtcatgatc atttcaacaa
     8941 gtacgtatcc tgggaatgtt ttatgaacga tttctttttc tttcccattt ttgacttcag
     9001 tttctttttc ttcaggaaca acgacacgga aaataaagtc ggccattttc atactttgcg
     9061 cacgagattc gatatttgct tttactttgt tttcatatcc tgaatatgta tgcaagacat
     9121 accagttttt ttcaaaagat tccattgaag gctcctcatt tcattgtaaa ataaaaaaac
     9181 ctcagtacac ccgaagtttt tctttcgctt cattatagca ctaaaagtac gttgtggcta
     9241 gcgagttatt ttaaaatcca gccaaataag ctttggatgg ctgtatccat tacgtaaaac
     9301 atcaatccga aaataataga agtttcgatg actactaacg tatctttacg gagttgtttt
     9361 ctgcttggcc aagttacttg tttgatttca tcttttacgc tttttagaaa tttcatgaga
     9421 caacctccct tgctactgtt tatttcgttt ctttatgtaa tgtatattga ttacaatatt
     9481 tacaaaactt attgatttcc aaacgttcgc cgctttttcc ttcactgact gattttgtgt
     9541 agttgcgaga cccacaaacg gaacaagcga gtgctgcttt tttctttgcc atttacttct
     9601 gcccccttgc tgagaatcag acttatactt cataacagta tcacgtaact ttttacttgt
     9661 caatttgtga aataaactat ctattgtttt ggataaaatt atgatatgat taatttgaaa
     9721 ttaagctaaa atgagacaat tggaggtata gcgatgctat taaaaacact tgtatataag
     9781 aaaaaagatc tgacaaccgt tacagagact agtacattag aagaagcatt aaaaatttta
     9841 gaaacttccg gataccgttg tgtgccgatc ttagattctt caggtaatat tttccgtgga
     9901 aacatttaca aaatgcatat ctatcgtcat aaagcaaatg gcggtgatat gacgcttcct
     9961 gtcactcatc ttttgaaaaa cgctacaaag ttcatttacc tggatacttc tttcttcaaa
    10021 gtattcttta cgatcaaaga gcttccttat attgctgtcc ttgacagtga aaatcatttt
    10081 tatggtattt tgacacatag tacgttgctt aatatgctct ctcaatcttg gagcgtagaa
    10141 caaggaagct atgtactgac aattgcttcc acaggaaagc aaggagattt ggctgctatt
    10201 gcaaaaatca tttcaaaaca cagcagcatt gccagctgta ttactcttga tatcgaaaaa
    10261 gacgaataca ttcgacgtac attgatcacg ctaccggcta acactgaagc gaaagtatgt
    10321 gaaacgatcg tcaaccactt agagaaaaag aattataaag tggtagaaat tgaaaatttg
    10381 caaaaccaag aagcataatc aaaaagcgaa aaaagaaaac agctaatctg ctttcttttt
    10441 tcgcttttta taattagaca cgcttttcaa aatcaagcgc gcgatctaaa taatacatca
    10501 atggtgctga aacagccatg acgataaact cacttaatgc aagcgtgcca taggttgtcc
    10561 aaaatgcttg gccacctgtt ggaactaaca tataagcaat cagtcccatg ctgatggtaa
    10621 agaagatcgt gttcaatact aaacgcatct ttacattttt cactttgttt tgcaataaag
    10681 ctgtcaggcc taaagaaagc aatgtttggc cgccaccaaa aaccacatcc atgataccaa
    10741 aaccaaagaa ggcattatag acgatgaccc cgcctaaaac tccccaaagc aatttacggt
    10801 taaagacaac taaatgattc aaactttctg aaatacgaaa ctggatcggc cctgaggata
    10861 caggcgcaac taaaatagtt aacgctagat aaagagccat gatgatgcca tttacaacga
    10921 ttacggataa ccgttgtctg cttttcgttt tttgcatttt ctctcctctt ttctcctagt
    10981 ttttttaacg cgggatggtt gatgaaccgc ggttatgtga aacaaacaat aattataaca
    11041 gacttttcat ttatttcaat ccgtcttatc tgcttttaaa aaagcaaaag cttaaacaat
    11101 gtaaacgttt gctaaaaatc gtgggatatg gtataaagta agtgtaaggg gtcataagaa
    11161 ttcccagtaa agcagacgaa gtaggatctg caagaacaga attgttgtga aacttaaata
    11221 aataaaaaag gatgtgggaa tttgagcaac ctcttactag agaacggtct agcaagcagc
    11281 tgaagatcta agtggacatg ccgaaccttg gaacttctcc ttacgtaagt aatggatcat
    11341 gcgcttgcta ggcttttttg tgtttttcgg tcttcggacc tttttcattt tcctagactt
    11401 ctgctataat aaccaagaat taattttgta acagattttt ttgaaaaaac aagtttcttt
    11461 cattcaaaaa atagaaaaag cttgctaatt attggttaac cagcaaacag gagtattaaa
    11521 atatcttgtt ttattgaata gcaggaggaa atcatgaaat tccattatca tatattgatc
    11581 aatccatcgg caggcagcgg aaatggctat aaagtagcag aaagaatttt accggtatta
    11641 aaaaataagc atattgacta tacacttcat tatagtgaat acaaaggaca cgatgctgag
    11701 attgctgaaa ctttggcaaa agaaacattg attccttgga tagaagaaga acagaaaaca
    11761 atcgatactt tccccctttt gatcatcgta ggaggtgacg gcaccttaca tcaggtactt
    11821 gatactttct atcagatgga agtagaattc ccagttgctt atattccagc aggttctgga
    11881 aatgattttg ctcgaggggc aggccttcca aaaaatccca aaaaaggact tcagctgatt
    11941 ttagcagctc aatcaccaga aaaagtccac ataatggctt atgaagaaaa aataagtgaa
    12001 aaaaaaggaa tagctgttaa taatttcggt ataggattag atgcagctat cgtccatgcg
    12061 acaaatcatt cttccacaaa aaagcgattg aacaaataca acttaggatc attttcgtat
    12121 ttattctcta ttctccgtgc tttattcact caaaaaggat ttccaattct ggtagatgca
    12181 ggaggaaaac aattaagttt ttcaaacgca tttttatgta cagcaacgaa gcatcctttt
    12241 tttggcggag gaatcgcgat tgttccaact gctgatgcgt ctaaaccagt cattgatttt
    12301 gtcgtggtcg aacgaatcaa tttctttaaa atcttgtggc tcattctttt acttatacag
    12361 aaaaaacaat tgaaatcgaa atatttccat cactacacaa caaacagatt acgtatcgtt
    12421 tcaacaacac cgcaacatgg tcaagaagat ggagaagaaa tggaaaaacg accttttgat
    12481 atcacaataa ccacaactaa acagttgttt tggtgttaat aaaagaggtt ttgtcagaag
    12541 ctacatctgg aacaccgttt attcggaaaa aaggactgtg acacgacttt tgtcacagtc
    12601 ctttttttac tatgcagtta attctttgcc caattgggtt gccatcgata gtttctttct
    12661 ttacccaaaa taaattggat cattgaaaac accgcagcaa aagcaactaa gacacaacca
    12721 ttgaaccaaa aagattctgc aaatgtttga ttgtaactga gtgtttcact gatcaagtta
    12781 ttattttgaa caaaccaata tgttggtgtg aatgccgcta ttttgctaac catttctggc
    12841 aagtattctg ctggtacaaa caccccaccg ataaagcaac tgcctgtgat aaaaatatct
    12901 ttgatcccac cgctggtttc tgagtttttt accaatgaag tgatacaagc cgaaaagcta
    12961 atgatcggca ataaaaacaa gaaagtattt aaaatatgat accataccgt tatatcccac
    13021 gaggtgtgga tcactaacag aaccattaga agtaagaagc cactgaacaa cagcagcgag
    13081 taactaatca aactaaaggt tactttgcga ttcaagtttc gtttggtaat gggcgcactc
    13141 tggtttcgtt tactgatctc tttgcgatta aacgctagat tgatgacgct gaaaccgcta
    13201 aaaatggata gaaacaaccc ataagacaag aaattaaagg ttgtgcccgt caacagcttt
    13261 ttcatccgct gactatatcc cgcacttagc tcaacttctc cagtaaccga gagtgccatt
    13321 tgtgtctgtt ccatagctgc atcgattgtt ttatcttctt cttgaaacag aatcagtgtt
    13381 tgcaaaaagg tgttcacttg ctgtgtaaca actgttttag aaaaggcatc tgggcgactc
    13441 tgtatcttga tcgtcgccat ctttcctact tcgacttgtt cggtaaagtc tgcaggcaga
    13501 tgtaagataa tgtttacacg attaaaataa agagcatcat cgatttcttt ttgtgaggtt
    13561 ccatccaatg taacgactgt ttccttgtca ttcaaatatt tacgaaggcc atcgactaca
    13621 ggcgactctt cttcactaat caaggcgatt ttcgcatctt gcagagaggt attttcaaca
    13681 tcaatatttt gcgcaaaaaa gaatgtggca acaacagtga taattacacc tatcagtaaa
    13741 ttcgatttat ttgctttaag caatgtccaa atcgttttat aaagcgtcat aacgcacccc
    13801 tctttccatc caagcatttc ctaacgcaaa aaggaaaata aatccgatca accatagaag
    13861 attggtataa aaactagcca aatcagtgta gaaataaagt ttatacaaac tttcgctgat
    13921 caagttgacg agatttaatt gaccaacaat cggaatatga agatcaatcc aatacttcac
    13981 ttgttccgac cccatcatac cagcaaaaaa gctcatcgca atcgtcactg aaatcaagat
    14041 gctatccttt tgcaccagat tcatttttgg tatcaaatta ccaaccaatg tacctaacaa
    14101 aatcgcacac aacgacccca gagcggtaac aatcatcaac cattgccaac gttcaccaaa
    14161 gtctacttga tagataaaac gaaagacagc taacataata taaatctctg tcaaaacaat
    14221 cgtaaagctt gctgccaaat tagcgattga aactaacgat tgctttctag gaatcaagga
    14281 caagcgaata ccatttgacg attgatttgc ttgctgatcc tgtacattgc ggacccccca
    14341 catcgtcccg tagagaatcg ccattccaat cagagtgaag aaataaaaac ttttgaaact
    14401 catattacga cctgcatgaa cttcttgaaa agatacttct tgttcaaaaa cagtcatgat
    14461 gggcgcgaga tcaacgttct ccacttctgc cactttactc gaaattatac caatacgctg
    14521 taaaaattga tccatgacgt ttttcatcaa cgtctgactc attcctgctt gacccacatg
    14581 gagcgaaaca gatgaaccat caatgagata aaaaccagcc aaatcatctg catttagctt
    14641 ttcattcgct tctacttccg atagttcctg tacttcaaat aatggtttat cattgacttc
    14701 gacttgttct aagactgtgg taaaattttc cgaaagcgct gtttgatccg ctttgattac
    14761 aattccagta ttaatcgttt caagattgcc accttgatca atctcgccaa aggccgccat
    14821 aaacatcaat cctaaaagaa ttggaaaggc caatgtccaa aacaacatgg atttatttcg
    14881 cagcagtatt tttgtgcgat atttataaaa atgccaaaac attatttccc ctcctctagt
    14941 ctctcaaatc cttgccagta atctctaaaa agacatcatt caaggacgct tctttggttg
    15001 ctatttttcc gtaactgatc ttttcttctt gtaagattgt aagaatcggc aacaatactg
    15061 tatttagttc agtagttgtt acttggagct gacctttttt ataagcgatt ccatcagaag
    15121 ctattgttcg ctgaatcgct gacaacacat tttctgggag taatgggatg tcgatcgtca
    15181 aacgatccgc cgtccgtacc atatctttta gagattcttt ggttccttta gcaatcacct
    15241 gtccttgatc catgatcacg atctggtcgc aaagaatttc tacttcttcc atgtaatgtg
    15301 ttgtatagac aatggttgct ccttgtcgat tcagctcctt gatactttct aaaattttat
    15361 tccgactttg ggggtctaca gctactgtcg gctcatctaa aaaaattaac cgcggcttgt
    15421 gggcaatccc acatgcgatg ttcaaccgac gtttcaagcc accactaagt tctcctggac
    15481 gaaacgaaac atatttttct aatcccacta attgaatcac ctcttcaatc aaggctttcc
    15541 ttttctcttt ttcggaaaca tataaaccgc aaaaataatc gatattttct tgcacactta
    15601 gttcgtcaaa gaccgcaacg tcttgaggaa cgaccccaat cattgccttg acagcataat
    15661 tttcaggagt cattgttgtc ccgaacaatt taatggttcc tttgtcataa tttaacaaag
    15721 aaagcataca gttgatggca gtcgatttcc cactgccgtt tggacctaac agacctaaga
    15781 cttctccttc ttgtacttgc agatcaaaat gattcaatgc tacaaaatct ccatagcgtt
    15841 tgactaattt atcaatttcg ataatcatca tgaaaaactc cttccaaatt tcttgttgat
    15901 ctgactataa taaagtgcaa gaaaagaaac tagtgacgat tggctaaacc acacatgaca
    15961 tttgtcacaa tgaaggagtc tgctatgcat aaatctcggc tactcaacga aactagcttt
    16021 ctactggtta tctcattgct tttgctggta agtcaaggat tcgttgctat gcaagttatc
    16081 tatttattga tcgccgtttt attcactggt gtcagttatt tactgcctac aaagccaaat
    16141 tggattttcc aagttattct atttctttta tgtgcctttt tacctgatct tgtcctgttt
    16201 ctgccagcga cgatgcgagt gatcgcccct gaagaaagcc accttgatta cttgtttttg
    16261 ttaggttttt tgttgatttc gacggttggt aatcatcccc ccatcaaggt cgcctttgtc
    16321 gtaattttgc tgtttttttc cttttactta cgaaaaaaag atcaacgaac gaccgatctt
    16381 gagaaaaagt acattgtctc caaagatact cattgggaaa aacaacaaga gttatcggaa
    16441 caaaataaag cattggccct gtcacaagaa acacaattag ccttgcaaat tgtggaagaa
    16501 cggaaccgaa ttgcccgaga catccatgac aatgtcggtc atctgctgtc aagtgcgatt
    16561 ttacaaattg gtgcgcttga aatgatcaat caagaaccaa aactaaaaga accattagaa
    16621 aatttaagtg ccaccgttca cactgggatg gatcggattc gtcaaagtgt ccatgacctc
    16681 catgagacat cgatctcttt tcaacactcc ttagagtttc tgatcgctga ttttccttct
    16741 tctgttacga ttgaaggaaa cttgtttgaa ggattgaatg aaacgcagca aaactgcttc
    16801 ttaatggtca tcaaagaagc cttaaccaac agtatcaaac atagtcatgc caaaaccgtt
    16861 gttttacatt ttcgaacgtt gccggccttt tatcgcttgc aaatcacgaa tgatggcacc
    16921 agcccacaaa aaaattctgc tcccggtatt ggtttgaaaa cgatgcgtca acgtgttcaa
    16981 gaacaaaagg gacaagtcca tattttccaa gaaaaccagc aatttcagtt aacgatcatt
    17041 ttaccaaggg agggacacca atgataacag tagtcattat tgatgatgat ccatttgtga
    17101 cgaaatcgtt acaaaccatt ttagaaagca cgaaagagat ccgtgtgtta ggcattggtc
    17161 attgtgccaa ggatgcgcta gtcttatacg aaacccatcg tcccgatgtt ttgctgacgg
    17221 acatccgcat gcctaaacaa acgggcatcg atgctgccaa agaaattttg gctaaatttc
    17281 ccaaagccat ccttttgctt ttgacgacat tcaaagatga agaatacatc cgcgaagcat
    17341 tttccatcgg tgtcaaaggg tatttaatca aacagaactt gcaagcgatt attccttctg
    17401 tcaaatccgc ttataatgga caggccgtat ttggtaatga aatcatcgag actttcactc
    17461 aaatgatgaa gaataaaccg acgattgata gtcatgctta tgcttctttt agtgaacgtg
    17521 agcttgcaat catcaaagaa gtagctgccg ggaaaaacaa taaagaaatt gcagatgcct
    17581 tgtacttgag tgacggcact gttcgcaatt acatcagcca attactagaa aagctagatt
    17641 tacgggatcg cacgcaattg gctatctatt tttatcagca tttgcaataa gtcttatcta
    17701 acacaaaaaa acctgaatga tgctccttcg catcattcag gtttttttca catcttctat
    17761 tcaattgtcc cgcaaaccat ttcctcacga ttgaaagaat caaggattcg cagggctgcc
    17821 aggattcgtt ggattgttcg gcgtgttagg cggaaaaata ggcgccttgt ctaacgtttt
    17881 ttctagtttt gattcttcac gatcgtctcg ttctacatca ggactttcat tcaatttgaa
    17941 ttctttttcc atctcatcta actttttaaa gtcattgcca gattcttttg ctaatttcgc
    18001 taatttatct tcggtttctt tagccataaa gtaacgcccc ccttttgatt tcagcttagc
    18061 atatcacact gaaaaaacaa aagaaaacgc taaccaacga tcatttttag ctatttctca
    18121 tctttaaaat caagcatttt ttgccggtat aaaatcgtag ggagaactgt tttccatacc
    18181 aatcatcgga tctactaact ggttatcgat cacttctgct gtaagaacga aatctttaga
    18241 aacagcaggt tctacctctt ctttcttttc ttctccaaca aatgaagaaa acagatctgg
    18301 ttccactgtt tccattactt ggtttttcgt ttcattcgtc ccactgtcat cttctacagg
    18361 tagcttttct tttacaaccc gtggctcttt tgtatagttc ccatcttcaa atggatcatt
    18421 tccgaccaaa gaatcttcat aagctgctag tttggtccgg agagtgatat tcattttatc
    18481 attatccatg atccttgatt tcaacgtagt catccggctt gctgattcat tcgctacaca
    18541 agtgacatag gcttgctctt cccctaacaa aatcaacagt tccttactgc gagtcactgc
    18601 cgtgtagagc aaattacgct gaagcatccg ctgatattga tggaccatgg gcaaaataac
    18661 catcttgaat tcactgcctt gtgatttatg gattgagcaa caataagaaa gagtgatttt
    18721 attccattcg tttcttttat aagtgacctc atttgcatca aactggataa ctaattcatc
    18781 cactttatct tcagaatctt ttgccaagat gatacccgtg atttctccca tatctccatt
    18841 gaatacattc aattcgggag tattgactaa ttggaggact ttgtctccta tccgataaac
    18901 ggtgtcgttc caatgtactt ccttttttcc aggtttccct ggattgaata tctcctgcat
    18961 cattttattt aacgcatcga tccctgcagg tccccgatac attggtgcta atacttggat
    19021 atcttgtgca cggaatccct tatttttcgc tttttccaca attttagaga tcaagggttc
    19081 aatttgataa gcatcacttc ggaaatagga acgatctttt tgattctttg tgaaatcttt
    19141 tggcagacgt ccttctttga tctcatgggc aagaggaata atactagaac catcaccttg
    19201 ccgataaatc tgattgagtt ccattttggg aatttgttcg atttccaaaa gatcatggag
    19261 tacttgacca ggaccgacag agggaagctg atctttgtct cctactaaga taacttgcat
    19321 attcgtcgga atcgctttga ataatgtgtt agccagccat gtatcgacca tagacatctc
    19381 atcaacaatc aaaagtccgc cttctaattc tttcgtactg actgaaggtg ttttttcccg
    19441 tccattcagc cccagaagac gatggatcgt actggcaggc agacctgtcg tttcgttcat
    19501 tcgtttagct gctcggccag ttggtgctgc aagtagaata ggaaaaattt cttgggtata
    19561 ttttgaagga tccaattcca gtccgttcaa ctctgcaaaa agattgacga ttccgttgat
    19621 cacagtagtt ttccccgtac ctggtcctcc tgttaaaata aatagagggg aacggattgc
    19681 ttcagcgata gcttgttctt gcgaaggtcc atactgtatg ccgaatattt tttcgatacg
    19741 ccgaatattc ttctgcactt ctttttcagg ataatggatt tcttttttac gactgagaag
    19801 gcgctggatc gaattaccga tcccccattc agaaaagtac aaactgtttt cgtaaagatt
    19861 agtctcttct tgttgtatct ttccttcttc aacaagttgg ataattacag tagcaacctg
    19921 atcaggctgg atttcaattg ggcgacttgt ttctaaaata cggatcgttt gctgaagcaa
    19981 tgtctccgcc ggtacgtatg tatctcctgt ttgaactgca tgctgaaaaa tctgatgcaa
    20041 gatagctgct cgaattcttc tcggtgaatc tgctgcaata ccgatttgtt cggctagatt
    20101 atctgcacgt ttgaacccga ttccttctat gtcttcgact agctggtacg gattttcttc
    20161 gatgatatcc aaagactcgt tcttataggc ctggtagata ctgaaagcta actgactacc
    20221 gaacccgtaa cgattcagtc ccatgatcac ttgatccatc ccgtggttta gccgaatcgt
    20281 ctcagcaagc atttctcttt ttggcttcgt caaaccggag atttgtttta ataccgaagg
    20341 atcagcaatg attttttcga tagcatcttc tcccagcaat tctacaattt tttctgctgt
    20401 tttcttcccg attcctggga atttttcgct ggaaagatac gctacgatcc catttgcagt
    20461 tgttggcttt tcttgctcat atctttcgag tttaagctgc cttccgtatt taggatgatc
    20521 cactaattct ccataaaaac gataagtctc attttcttga atatcgccga aacttccggt
    20581 gactacaatc tctttttcac ggtaatcagc tgtggtttca ctgatgtgta ccaaaagcac
    20641 tttgtaaaaa ttactaggat tttgaaaaaa aatcgcggca actgtaccaa cgaaataagg
    20701 catttcttct ggctgcatcg gaagccgcct ccttcttcta tagtaaaggt tctggtgctg
    20761 tttcagttcc taaaaaatct gtttggttcc atactttcag ttcatatggc aataatctgc
    20821 aaggctcttc agtctctaaa atcgtcaaac tactattgaa caatccaccc atttcacgca
    20881 actcacttag ctcttttcct gtcatccatt gaatggctgc agttaaagaa gctccatgtc
    20941 ctacgaataa aagtgggcca tctttatgtc gggcaaccgc atctgctaca acagtttcga
    21001 tacgtgtgat tgccgattca atcgtttctc cgtgaaaagg agaaggatca tacagatcta
    21061 aatgattacg caaattcggc agattatccg gatatttctg gtacatttca tcgatcgact
    21121 gaccttctaa agtacccagc cctagttctc ttagcttatc tgtataaaaa atctctaccg
    21181 gacgagcaag ctctttgttg ataccttctg ctgtatcacg tgctcgcttt gctgtactgg
    21241 aataaattgc tttaaaagga acatatttta cctttttccc aaatgctttg atttcttcgt
    21301 aactttgtgg caaaagcgga gaatcgccat tgctcccttg aaaacgtctt tcctgattcc
    21361 attgtgtctt cccatgtcgg gtaaaatata gttccatacg cagcaaactc ctgtctttct
    21421 tttattttac tctataataa actgtctaat ccagcttgtc attttcatag tgatgcgtca
    21481 attctaaatt agggaatcct tgctggcgaa gtgcttcata cactatcatt gcagctgtat
    21541 tcgataaatt caatgagcgg acatgctcat cattcatagg gatgcgtaaa cacttttctt
    21601 cgttttcacg cataaatgtt tctggcaaac cggttgtttc ttttccaaat aaaaagaagt
    21661 gctcttctcc atcagcaaaa ttttcgtcac tatatacttt atgggcaaat tttgagatca
    21721 aatgtagttt acgattacct aaaaatgtta aaaaagcttc taaattactg tggtaagtaa
    21781 tatttacatc attccaataa tccaaccctg cccgttttag atgtttgtca tcagttgaaa
    21841 aacctagtgg ctcgattaaa tgaagcggag tattcgtggc tgcacacgta cgggcaatat
    21901 ttccagtgtt tgcagggatt aatggttcaa ataaaacgat atgattcatc aggatcattc
    21961 ctctcatatt tatgttcgtt tctattttct gacaatttga tttttgcata aaaaaaaacg
    22021 tatcaactcg gtgtcagtca ctgcgagtcg ttacgttagt gaagctcttc tcactaactt
    22081 ttatcagaac aggaacctga taaaaaccaa tgaaaaaaca acttgttgct aatataacac
    22141 cctcaacttt tttttgcaac ctctactcat taaaaactgc tagaaaaacg ttttctctta
    22201 gttcctttta ataattctat gtcaattgta atcttcttca aaggattctt atataagtct
    22261 gctttgattc gctcttctac ctgccattct aaaggtgaca tggccaaaag atctttttga
    22321 ttttcctctg gtatctcaaa ttcataggtt acacgctgac gttcagttaa tttcattgct
    22381 tcagcaaact tggctactac acgttcattg gaataactct gtttttcagg ttgattcgga
    22441 taaaaagcct gacgtagttc ctttaaataa ttttcttgtg gcaccacctt caacacttct
    22501 ccatcttctg agagtacacg ttggaattct tggtaatgag aaggcgaaaa gatatttaaa
    22561 accgcgttga atgtttgatc agcaaacggc aaattggtca aatcagctac acaccaaaat
    22621 gcttcggcag attgttctgt agctgcgtag actccttctt ttgaaatatc aaatccaaca
    22681 gcattttgaa tctttttcaa ttttgaaagt tcattcaaga agctaccttc tccgcagcca
    22741 acatctaata gcgagacttc ttcaggtaaa tgctgcgcaa tagtctcaat aacgggctga
    22801 tacatgccag attggatcat tctacgcctt ggaacgaaca ttccttgatc gtattctgtt
    22861 ttaaccgcat gggataaaaa ataaagtgtt ccttttttgg aaaggtcaaa acggtgatta
    22921 tttttgcata tcaagccatt atcagacttt tttacttctt cttggcaaac cggacagcgg
    22981 aacagctgat gattttctgc caaaaactgg cgtcctcggt caattttttt taacatagtt
    23041 ccccctcatt tctgttttat cttaccgtta gatgctcact ttggcaactg ctaaaaatat
    23101 gatacaatgc ctagaagaaa gtgaaagata aaggagaaga ttagatgatc aaagaatttt
    23161 gcgctgaaaa ctacacatct attcctctag caattgctag aggagccgat cggatcgaat
    23221 tgtgtgacaa cttagcggtc ggtggcacaa ccccgagtac tggtgtgatc gaagaaacgt
    23281 taagttatgc tggggaaaaa gatatccccg taatgacgat tattcgtcct agagggggcg
    23341 acttcgtcta taacgatatc gaattgaaaa tcatgcatac tgatttgatt gaagcaaaga
    23401 aattagggac ggatggcgta gtatttggtt gtcttactcc ttcaggctgg ctagacgaag
    23461 aggccttaga actattgatc gaaacagcag aagggttgca gatcaccttc catatggctt
    23521 ttgacagtat tcctgaagat cggcaattcg aagcaatcga ttggctggta gaacatggtg
    23581 ttgaccgtat cttgactcat ggcggtcctg ctgacaaaac tattgtagaa aatttcccac
    23641 ggcttaaaga attgattgcc tatacagatg gccgcttaat cattttgcct ggtggtggtg
    23701 tcactgcaga gaatgcagca catgtagcta aagaattgaa cgtaagcgaa gtacacggaa
    23761 ccaaaatcgt ggatcttcag ttataatctt ataaacaaaa aaacatttgc aacaggcata
    23821 atgcttattg caaatgtttt tgttttttta cataaaatag cccataaata aagaagcaat
    23881 attgaaataa atcagtacac tcgtcaaatc gctcaaagtc gtgataaagg gaccgcttgc
    23941 tactgctgga tcaaatccta gacgatccat tagcatcggg attaaactac ctgcaagatt
    24001 cgccactgtg attgcaaata gcatagccat ccctatgaca aaccccaaga taaaattatg
    24061 tttccaaaaa cctacgatta gaaaaatcgt gatccccgta acagctcctg tgacaacgcc
    24121 ggtcaagact tcagccaaaa caagtcgaac aaatccatga tctttctcat cagaaatcgc
    24181 aagtcgacga acagccacag ctagcgactg ggttccagca ttaccggcag ttcctgtaat
    24241 cagcgaaatg aagactgcta aaatactcgc ttcacttacc aagtcttcat agtgactgat
    24301 taaagaagcg gtcgacatac ctaaaaacaa cagagtgatc agccaaggca aacgtcgaga
    24361 cgctgccttt actggatttt cattaatttc ttccacattg accccggcaa gtcctgaata
    24421 atcgctagca gcttcatcat caataacgtc gatgatatca tcgactgtga cgattcctaa
    24481 caaatgatca tcataatcgg taacaggaag agctaagaag tcataatctc taaatgtttg
    24541 tgccacatct tcttggtcgt cgcctacatg aactgaaagc actcgttcgc tcatcagatc
    24601 agaaatcatt gcgtcgtcat cattcacgat taggtcgcgc agagaaataa cccctactag
    24661 ctgaccttct tgattaacga catagatgta atagattgtt tcagctacat ctgcttcgtt
    24721 cttcagcacg tacatcgcag agcgaacagt ttgattggct acgatcgaaa caaattcagt
    24781 tgtcatgatc gcgccagccg tttcatcctc gtaatggaga agttccttga tttcactggc
    24841 atcgtctgcc gacatcaaac ttaaatattt agcaatttgc tttttatcca gcgtattaag
    24901 caagtcaaca gcattatctg tatacatctc agaaagcata tcagctgcat agctaggacg
    24961 catttcatct aggtattctt tcatatgttc gttgtcttct tcaatcacat cgaacatatc
    25021 cgctaattct ttaggcgaca gataggaata gatcagctgc ctgttctctt ctgtaagaga
    25081 ttggtagaat tgcccctgct catagatatg catctctaaa aaactgtttc tgaatgcctg
    25141 aatattattt tcccttaaat cttgagtcaa ctgggcaaat ctttcctcta attcttgatt
    25201 ttcatccaaa ccaatctcac ctcgccttat tcttcaaacc gtctcacaac tgcctccatg
    25261 tctgcggcta aaggctgctt gaactccagc aactcttctg taaatggatg gtaaaaacgc
    25321 agatcataac aatgaagggc ttgtcttttt attcccagat ccattcgccc gccatacata
    25381 tcatcaccga gcagggcaca gccaattgcg gaaaaatgaa ctcttatttg atgtgtcctt
    25441 cctgtatgca gctggatatc aactaacgcg atttctgtta gtcgcttctc tatccagtac
    25501 tcagtctgtg ccgaacgtcc ttcatcagag accattcgct tcaatagcga agaataatct
    25561 cgggcgatcg gcaaatcgat atgtccatgt gccgataaat ctgaaagcct tccactgact
    25621 aaggcctgat attttttcac aaacttcttt tcacgaagtt gcatatctaa ttttgcgtgc
    25681 gcaaatccat gctttgcaaa aagcatcaag ccagatgtat cccgatctag tcgcgtcacc
    25741 acatggatta cttggttttc gtaaccttgt tttttataat aagactttac acgattagcc
    25801 attgtcccat ttggatggta ttgggcagga atagacgaaa cgcctgctgg tttatttact
    25861 accagaacgt gttcatcttc atacacgata tcgatcggtg tttcatccaa aagtacggtt
    25921 tcatgttctc cttcagcagg aatcacaatg gttaccttat catccttagc tagagaaaac
    25981 aagacatttt ctacttgatc attaactttg atttgaccgc cttgaaattt tattttcgct
    26041 aacagaccct ttgagatccc tttttctttt aaaaaatatt ttacttgctg agaatgatct
    26101 ttctcataat cccatgtaaa ctgcatatca atcctcacta ataaaggcat ctttcacacg
    26161 atgccaaaaa tgcatatgtc gataagaagc aaaatggatc cgttcttctg ctattcgata
    26221 ataaattgag cggatatttg cttttgcgac attcaattga tcaaccgtta caagataatc
    26281 ttctgtattt tccaatttta tttctagcca ttcatcatgg gcaatcacga tcggagaacc
    26341 caaagtacga aacactcgat tattcagtga agcaatctct gccaactgaa aggcattgat
    26401 actaggatgg atcacagcac cgccgacact tttattatat gctgtcgaac cagtaggcgt
    26461 agaaatcgtc aatccatctc ctctaaaact ttcaaacagc tcgtttttga tgtatacatc
    26521 ggctaccatc gtacgattgg cccgtttaat cgttgattca ttcagtgcta aaaaatgctg
    26581 gtccggtgtt tcatccaaat aactgatccg aacatccagc aagggataac taacactttg
    26641 ttcccggttc gttcgtaagc tgtctactag ttcttccagc tcgtaatccc gccaatccgt
    26701 gtaaaaccct aaatgacctg tatgcacgcc caagaaacgc acatcattca gacaatgact
    26761 gtagtgatgg aacgccgaca ataatgtgcc gtctccgccg actgagatga ccaattctgg
    26821 ctcatgatca tctcgttgaa tccctgcttg ttctaaaagt gcgttcaaac gttctgtcac
    26881 ttctatagtc tttgcttctt tattatgaac gatcgctact ttcatcccct gcctcctctc
    26941 tttcgctttc tttcttcaaa agcgttcgat agtagacatc atctgatttt cctcttccat
    27001 gggagaataa ccgctgtgct tcttggattt cttcacggat actggacatc tcttcatcca
    27061 actgataggc agcttctgcc gcacgctgaa gtcgttcact catttcttct ggaaaaacgc
    27121 cttgatattt ataattcagc gaatgctcaa tcgttgccca gaaattcatc gcaagagtcc
    27181 gaatctggat ttccgccaaa attttcttct caccgctgat cagctgtaca ggatactcga
    27241 tcaccagatg ataagaacgg tagccacttg cttttttatt tgtgatgtaa tctcgttctt
    27301 ggagaacttt catgtctttt cttttacgga tgatctcaac aacttgatgg atatcttcta
    27361 caaactgaca catgatcctt aacccggcaa tatcagacat ttcttcttct aaacgtccca
    27421 ttggaatatg ccgtaattgt gattttgtta caatactatc gactggcttg actctcccag
    27481 tgacaaattc aatcgggaca tgtcgatttt gttcacggaa ctgtttgcgg atcccccgca
    27541 gtttcacttt caattctgat acagtttgtt catatggtgc taaaaatgct tcccaatctc
    27601 gttccatagc tgatacctct acttcattaa cattcattgt ttattctacc atatatgcaa
    27661 agttttttcg tactcttgat ttatgcattg accaaaaaaa gaaaattagg caaaatagac
    27721 aaaaaggagt cgatcaaatg acacaaatgc ttgaaatcga atataaatct atgctgacaa
    27781 agcaagagta cgagatattc atcgctcact atcaattaac agataaagat ttccgtgtac
    27841 agactaatat ttatttcgat accgttgatg aacagctgaa gaaacaaaat tgtggcctac
    27901 ggatccgttt cgttggtcat caagctgaat atacattaaa aacacctgct gaaaaagggc
    27961 gtctcgaaac aacggatacc tttataacag aagaagcaga agcatttatc ttcgaaaaac
    28021 gtctgcctcg ttcaggcgca gtttttcaaa aactttctga tttgaatatc gatcctgctt
    28081 cattaatcca aaccggaaag cttacaacaa aacgagctga attccctatt gaagaaggac
    28141 ttttagctat tgatgaaagc tggggagaca atctacacga ttttgaactg gaactagagg
    28201 taggggatgc atctgtggga aaaattgctt ttataaattt tttaaaacgt ttttctttac
    28261 cttatcgtcc tgctaagaac aagatccaac gtatgcttga agctaaaaat tagaaacctt
    28321 tcattttcac aaggcacttg agttcatttt tttacttttt tctgttaagt tataggatgt
    28381 gaaagaatct agtcactagt tgtatagaat ctattttggg ggagattcat atgatcgaaa
    28441 tctatttatt tgttaatcct ttaggggaat actgtttcga aatggaacaa cagttattac
    28501 aatttatcga agaagagtac gggacaccat caaaagaaaa gatgcaattt cgctttcttc
    28561 ctttggtcaa tttgcagaca attggggatg tcatgcaaca aaaaggaatt tctcagaaca
    28621 atcttgagga acgtaatcac ttattctcaa tgacttattc tgcagcattg gactgcaaag
    28681 cggcgcagtt tcaaggaaag aaaaaaggcc gccgtttttt gatcaaattg caagaagcag
    28741 tcggctgtaa tgacatacca tattctcaag aacttgctga atccatattt gaagaaatcg
    28801 gcggcgattt gaatatgttt aaagaagatc gtcagtctga cttcgttaaa gaagtattct
    28861 tttctgatca aaaaattgct cgggaaatgg gcatcacaaa acacccttct gcagtcgttt
    28921 acaattatgc ctgcgaccgt gattttggtg ttttagtaga agtaaaaggt acaagtagcc
    28981 tttggagaat cacagagctg tgtaaaacaa cagataactc atatcaagtt tttaatggtg
    29041 aacatttaca tacaaatgat tctcagttcc attcaagaac tagccattta cacttgcttt
    29101 ctaattaa
//
LOCUS       DAXWOO010000016        28676 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.016, whole genome shotgun sequence.
ACCESSION   DAXWOO010000016 DAXWOO010000000
VERSION     DAXWOO010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28676
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.016"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(17..715)
                     /locus_tag="ACGE70_000578"
                     /pseudo
     CDS             complement(17..715)
                     /locus_tag="ACGE70_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            794..970
                     /locus_tag="ACGE70_000579"
                     /pseudo
     CDS             794..970
                     /locus_tag="ACGE70_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1146..1715)
                     /locus_tag="ACGE70_000580"
     CDS             complement(1146..1715)
                     /locus_tag="ACGE70_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFF8692238.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYKTLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1789..3078)
                     /locus_tag="ACGE70_000581"
     CDS             complement(1789..3078)
                     /locus_tag="ACGE70_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692239.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFYFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLIKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQALAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            3405..4280
                     /locus_tag="ACGE70_000582"
     CDS             3405..4280
                     /locus_tag="ACGE70_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFF8692240.1"
                     /translation="MGKRSTIQLMIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGT
                     FSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLE
                     AMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDD
                     ELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAI
                     QLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKK
                     QIIHTLVAQIPIPVR"
     gene            4294..4617
                     /locus_tag="ACGE70_000583"
     CDS             4294..4617
                     /locus_tag="ACGE70_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692241.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KNKRF"
     gene            4614..5366
                     /locus_tag="ACGE70_000584"
     CDS             4614..5366
                     /locus_tag="ACGE70_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFF8692242.1"
                     /translation="MILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKR
                     EISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKL
                     SAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPELLILG
                     NRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKD
                     RTAYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5356..7527
                     /locus_tag="ACGE70_000585"
                     /pseudo
     CDS             5356..7527
                     /locus_tag="ACGE70_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7639..9810)
                     /gene="copB"
                     /locus_tag="ACGE70_000586"
     CDS             complement(7639..9810)
                     /gene="copB"
                     /locus_tag="ACGE70_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFF8692243.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(9830..12016)
                     /locus_tag="ACGE70_000587"
     CDS             complement(9830..12016)
                     /locus_tag="ACGE70_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFF8692244.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(12016..12225)
                     /gene="copZ"
                     /locus_tag="ACGE70_000588"
     CDS             complement(12016..12225)
                     /gene="copZ"
                     /locus_tag="ACGE70_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFF8692245.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12238..12678)
                     /locus_tag="ACGE70_000589"
     CDS             complement(12238..12678)
                     /locus_tag="ACGE70_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFF8692246.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQKGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12752..13250)
                     /locus_tag="ACGE70_000590"
                     /pseudo
     CDS             complement(12752..13250)
                     /locus_tag="ACGE70_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
     gene            complement(13535..15001)
                     /locus_tag="ACGE70_000591"
     CDS             complement(13535..15001)
                     /locus_tag="ACGE70_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFF8692247.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(15312..17393)
                     /locus_tag="ACGE70_000592"
     CDS             complement(15312..17393)
                     /locus_tag="ACGE70_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFF8692248.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNELIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            complement(17535..17657)
                     /locus_tag="ACGE70_000593"
     CDS             complement(17535..17657)
                     /locus_tag="ACGE70_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692249.1"
                     /translation="MNLDKLPATGFKLSCYPVKIKKASAGWIRAVAMIEEKKKE"
     gene            complement(17917..18276)
                     /locus_tag="ACGE70_000594"
     CDS             complement(17917..18276)
                     /locus_tag="ACGE70_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFF8692250.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     AFLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(18306..18647)
                     /locus_tag="ACGE70_000595"
     CDS             complement(18306..18647)
                     /locus_tag="ACGE70_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692251.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVGQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(18644..19318)
                     /locus_tag="ACGE70_000596"
     CDS             complement(18644..19318)
                     /locus_tag="ACGE70_000596"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFF8692252.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWREDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQKAGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(20252..21550)
                     /gene="asnS"
                     /locus_tag="ACGE70_000597"
     CDS             complement(20252..21550)
                     /gene="asnS"
                     /locus_tag="ACGE70_000597"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFF8692253.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVPEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(21590..22780)
                     /locus_tag="ACGE70_000598"
     CDS             complement(21590..22780)
                     /locus_tag="ACGE70_000598"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFF8692254.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(22801..23328)
                     /locus_tag="ACGE70_000599"
     CDS             complement(22801..23328)
                     /locus_tag="ACGE70_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFF8692255.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(23375..26164)
                     /locus_tag="ACGE70_000600"
     CDS             complement(23375..26164)
                     /locus_tag="ACGE70_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFF8692256.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIEAGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSSHTGMDTSRFIYHVLEEMKENPEPLDLSLEIIDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIMEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPGQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHAFFADVAHRGTGFLARNQSFYKQDFVRFLQKKIKQSNFLIVNHAFLVQETQRKEP
                     LLPASPYLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICVAEGLMNLVSSKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAMDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLLDFQAAMLPSTVWYPRYDHILYIGGTLKIGP
                     NRQYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLEAALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAIDEI
                     YQDIHEFLKKNEEGS"
     gene            complement(26311..26511)
                     /locus_tag="ACGE70_000601"
     CDS             complement(26311..26511)
                     /locus_tag="ACGE70_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFF8692257.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(27102..28208)
                     /locus_tag="ACGE70_000602"
     CDS             complement(27102..28208)
                     /locus_tag="ACGE70_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692258.1"
                     /translation="MAKKEKELLSQLTSEGTKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKITSLLDDPSFWRSATTSVAVIVSPEQVFIHRLGI
                     PVDDQYYVSDLPYLLAIIKNGHFNYSYYLLGLNRDSMKLYEVTNKQVKEVALPEDAPR
                     DVVTALGSELTGGGLNYASNGSEGTTFHGVNPKDEEVEIDWVNYYQAVDDYLKDTFEN
                     PEKWPIFLFALPENQTMFKKVAKNPYYRSDVAISASPAQLSLKEIEAGAKKLSQELAV
                     KEASDYNKLLDKKFIDQLVDIIPAAKEGKISHLFIATSNLADGFGEDPETEYDRRQVL
                     NELADNVITNGGQVFILEQKDAPDEKSLVAILRY"
BASE COUNT         8057 a         6080 c         5004 g         9535 t
ORIGIN      
        1 aagagggggg gattttttat cgttgtggaa tatttaaata ccaacgcttg tcatgaaaat
       61 cttgtgctcc acccttagta tttccttctg gatcgtttgt cgcacgcatc atgacgtata
      121 ctttcttatt agggaaatca cgcatattga aagatacatg atagccaaca tttcctaaag
      181 tattataagc ttgatttacg tctggtctat aaatcccatc agctcttact ctagctagtt
      241 cttttccagt attgtagtcc ataatgaaaa tatactcgta tttataatta gcaatgtgcc
      301 atccagctac atgcaagttt gcgttttcga tttctccgaa ctgatcaatg tgggcgtaat
      361 ttgttccatc tgtcagcgta gaatttgcag cacctgctcg agttggatca attactggtt
      421 tatcctccga agtagttgga ttatcatccg taaaaccatg agccaaatca taggctagtt
      481 tttctttact tacacccatt tcagaaagat aaccgtaagg atctgtatga tcgccccaaa
      541 tattttgtgt tacccataaa tgcgatttga ttcctggttg gttataagga gtgtctaatg
      601 ttaatggaat accatatttc attgctgaat ctcttgccaa ttcaacgtat actttatagt
      661 tcttttcaaa agtttcttta tcatgtgtgt gttgtaactc gatttgcaca ggacttttgg
      721 ctcacgaggt tgtcctactc tacatcttgc tgatagatat gctgaaagga aaacaccaac
      781 attatgtttt gctttttcag ctagaacgag acaagcagga atacctttag aagatatcaa
      841 agatttttta ggtcataaag atgtttccac tacacaagtc tatgcccata tttctcccga
      901 agtgaaaaaa agatcaatga atcaactaga aaactatata gaagagcaaa taaaaaagca
      961 ctcaaattga gtgctttttt aagctcacta tcactaaaac tatcactaat tttatttata
     1021 agcgaacatg tttctatcac tgatttttta aatctagcga aaaaccctta tcttaaaggc
     1081 gttcgttcaa ttctttggca aggtcttcaa agcctggctt gcctagaagt gcgaacatgg
     1141 ttttcttata gctcatgtag aaaaattttc ttgatgatga ttttatccat caattgagat
     1201 tgactgtttt gctctcctat gatttcagct atgattgtcc gattttgcgg ttttcttctt
     1261 aactggtagt tgattttccc agtttccatc aactcgctaa gcatatccac gactccgcca
     1321 aatccggtct ttttcgtaat aaaacctagt gctaccattc gttcatctag attttttgct
     1381 gtaatcgtca caccagtaat gagcgagtgc tgtaatgttt gggccagctc ttccatccct
     1441 tcagcattgg atacgacgga agctgttttg ttaataggtt cttcgtttat ttcttttggt
     1501 tctttcctct ccactttcac tgtttgacga aggacatcta gttcttgcaa gagcgttttg
     1561 tattgttctt gggtcagcat cacgccagct actttttcac gattaaatat atatacgcca
     1621 gttcccgcgt ctcgcgcttg agcaaataca tccatcggcg atcgtttcac ttctgtgatc
     1681 gaagatgtgg gaacttctaa tttctttaat tccaatggat ttcctccttt actaatcgct
     1741 ttttattata gcacagactg acttgttttg atcttttttc caaaggtttt atcggttttt
     1801 gcctaaccag taattgatat cggtaagaag tctttcgata ccaaatgaag gatggtacag
     1861 atacgttttt tgatcttcgt tatggtctag tggaaaaaaa tgatcggtga tcactagatc
     1921 tgcataccga ggcggcttat ttcgagtaca tacatgtata gagatccgtt ttcgatgtct
     1981 tagcgttttg ttcagtaccc cagcaagtct tcttgcttct tggtgttcat agcgaaaaaa
     2041 aaagacgatt ttcacttcag gagggtggac atctaaatac cctcgttcct ggagtgtttt
     2101 cagtaaaagc atcccccact ctactgcggg gtgtgcaagt gcttgttgga tcgctagccg
     2161 aaattgatat agtttatctt tggaattggg aaatttttct aatgtttcct gataataacg
     2221 aacttcttct atcagccggt catggatacc aattacgtaa cgaagccatt cttggtaaag
     2281 tacttttatc agctttgatt tgctttcgct ctcaaattcg ctattcagcc aaaacagcga
     2341 atcaagcttt tcagtcacaa tcgtcaattt ccgatagtct tgtgtctctt ctatccgttt
     2401 ccaccgcttt tcagtaatct ggttacagct caagcgcaaa attgctagat aatttttttc
     2461 ttttattggc aaaatctgct cctctctaaa tagcaattca ggaaacaatt caaagaatag
     2521 tgttcgactg tctcctaaaa tctcgatttc tgccggatcc tttctttgct tcaataattg
     2581 gatgaacatc tcagaaaagg actgctctcc atcatctgca aaaaaatgcg gaaaatcttt
     2641 caaaaagtaa aacagcagat atcttacttt tctttctgaa ccaaagatct gtgctttttt
     2701 ataagaaaaa gaaaggtggt actcgccaaa ccactttctc aatcgctctt gcgtagaacg
     2761 atcgtacttc tgctcgtaag gttcatttaa tagcagattt ttcagtaaac aaaacggggg
     2821 tgattggatc agaaaaacaa acaagagata aggttcgcta tatccttgat cataaactaa
     2881 ctgaacggtt ttcttatgaa gaatcagctg aatgggacag tcgctttccg ccaaacgttt
     2941 cagatgccga tccactgttt taacatcgat tttcagttct tcagctaaat cagcttttcg
     3001 ccagatcaaa tctttcatta gattgattag tgaaaataaa ttctgtgcat tttttgataa
     3061 tagtttttgc catctcatcg atcatttctc acctgatttc taaacatatt gtttgatcaa
     3121 aatgagttca ctcctaaaaa gataaaaaac tccctttcta tttatttaaa tacgcaaatc
     3181 ttcctcatta ttttttattt tactcaattt aatcgtattc tcatttcaaa acgaatgtac
     3241 tttaaaaaaa cataataaga tcactttgct tgtaggatgt attcccttcc attacaatga
     3301 aataaatagc gattttatga gaaggtgatg tgaaaatgga agaaaaaata gaagaatcca
     3361 aacaaaaaat cgatcagttg atataagaat tagaaaaagc ggtcatcggc aaacgttcaa
     3421 ctatccaatt gatgatcgtg gccctattag caggtggtca tgtcttattc gaagatattc
     3481 ccggtgtagg taaaacgtta ctggtcaaag cactagcaaa agctgtacag ggaaccttca
     3541 gcagagtcca atgtactcct gatctgctgc ctagtgatat tcttggtttt tccgtataca
     3601 attcacaatc aaaagagttt gaatttcgtc cgggaccgat ctttaccacg atcttgttgg
     3661 cagatgagat caatcgtacg actcctcgta ctcagtcagc acttttagaa gcaatggccg
     3721 agaatcatgc aacgatcgac aacaatacat accctctaga caatcatttt tttgtcttag
     3781 ctacgcaaaa tccaatcgag tacgaaggaa catatccttt gccggaagca cagctcgatc
     3841 gttttttatt ccgactacaa atcggttatc cttcttttga tgacgaactt ttgctacttt
     3901 tggataaatt cgaaaaagtg ttggataact taaatgaagt attgaacagt tatgaaatag
     3961 aagaaataaa aacaaatgtg gcgaatgtct ttgtcagtcc cgaagtggca tcttatgcct
     4021 tgcagttagt gtcagcaaca agaagtcatg ctgccatcca gcttggcatc agtccacgag
     4081 gaagcctttc ctttattcag gcagcaaaag cttatgcttt aatccatggg agaaattacg
     4141 tcacgcccaa agatttgcag gatttagttc cttacgtgtt ttcccatcgc ctgatttttt
     4201 acgatcgttc attgaaggaa gacgaaaaga aacaaatcat tcatactctt gttgcacaaa
     4261 ttcctattcc tgtgaggtag attgaggtag acaatggaaa aaatcaaaga ttatttcatt
     4321 aattttgtgt tatttctggt atatttcatc attgtgcttt ttgctcttgt attcaacaat
     4381 tctttaggct ggttcttgtt ggcctttttt actctttatg tatcatggag cttgatttca
     4441 ttaagcagct ttctaaagaa actttcggtt acttgtacat tgcagccact cgtttatcgc
     4501 aaaaaaagta tgcaggttat tttttccata gcaaaaaaga gaactttttc tttacctctc
     4561 cctcgtttac aaatcggctt cccagcaatc tttgcagaaa aaaacaagag attttgattc
     4621 ttacaaataa accgcaagaa gtccaattgc tttggcagcc aaaagaaaga ggatgccatg
     4681 aaacattaac tgtcattttt acagcctatg atagtctagg acttttcaga aaaagtgcaa
     4741 aaagagagat ctcttttcct gtgactgttc tgcctgcttt ttgtaagatt caagcagaaa
     4801 aattacagag agttctggaa aaaaaacagc agctgaacgc gtaccaaaga ggtcttgatt
     4861 ttcgggaaca tcgcgcttat cgtccaggtg attctttcaa ccgaatcgat tggaaattgt
     4921 ctgctcatac tcaagagtgg ttataccgtg aatacgatta tcaagaagaa gaatgttctc
     4981 ctcttttttg cttttggggt tcgcccagtc caaaatttga acctctgcta gacttgtatt
     5041 tttctttatg gcatttgaga aaagaaaagc agccggaact tctgatatta ggcaaccgcg
     5101 cctttcatgg caaagacccc ggtcatacct attttgcttc tttggaagct ggcgatgaga
     5161 aagctttttt cgcacacttg aaaaaatatg caaaaaaaca aatcgtcctc tttgttcctg
     5221 agaattctcc tgaagtctca gaagctattg aaacattgtc aaaagatcga acggcttatc
     5281 ttgtttattt cgtagaaaaa caactcatgg tccaagaaaa agacaagata tgttcattgc
     5341 caggaggtga atggattgac gattaaacgg gaaaaatggc tgcgtggcgt ttactccttt
     5401 gtgatactaa gtgtaggtgc tccgatattt gtagtagcta atcaattgca aaacagctac
     5461 cttcttatcc taactattgg tgtcctatgc cttattgtct gctgtattcc taaaaaatgg
     5521 gtacgtttgc tgctttcttt gccagtaatg ataggttgtt tgtttttata ttttccttcg
     5581 aagacatttt ccatattatg gatcttacag tttttttcgt cagttacaga ggaattcttc
     5641 catctatccc aaggtaaact ttattatttg ccagaaaaga cagctttttt tctcataatg
     5701 ttgctgatct atttatttat tacattgacc gtggattacg actattggta cgcacccttt
     5761 ctttctttga tggcttactt catgatgctt accgtttttc ggaaaaaaga tttactgtac
     5821 gaaatgattg cggtagtaac tgttttgttc gtgtatctgg cagcaaggca atttttctcc
     5881 atccggttgt tgtcgggaaa cactcgattc cagtcattga ctacagttct gcttatttgt
     5941 ttcttaactt ttagcgctat cttgccttta tatcttccga aagtacacca atctctcgag
     6001 gaaacgagtg aaccgatccg agaatatctt aatgatgaag ggctttatga cacactccat
     6061 gagtatcaat taaccggaag tgcaagaaca ggcttcagtg agaatgatga acagcttggt
     6121 ggacctttat atgataatgg cgaagttgtt tttacagcga accaaaaagg aaatggctat
     6181 tggaagatcg aaaataaaaa tcattacacc ggttctggat ggatagaaga tcaggacgca
     6241 cccatccaac caatccaaga attccctgtt gacatcacga gctaccaaac cgttatagaa
     6301 gacaaagaaa acattacatt gaccctaaaa aaacaagctt tcttacctta tccttatgga
     6361 aatactacct tgactgattt ttcattagct agtggacaaa cgctttacta tttgcctgaa
     6421 agccagcgat tcattcctgt cccgaatgaa tcgacacgtt ctgcaacttt acaaacagaa
     6481 aggttagttt ttacgagaga agaattaaaa agcagtcagt taaaagctga tgatttagaa
     6541 aatcagcaga caaatcttca attgccaaaa agtttgccta aacgtattga ggacttggcg
     6601 gtcgaattga ccaaaaatga agcaacttta attcgtaaag tcgaagctgt ctaacagtat
     6661 ttaaaaacaa gcggaaattt ccgctattcc aaaacggata ccgggcatac tccgaaagaa
     6721 cacgattatg tggattattt cctctttgat tcaaaagtcg gctattgtga caacttttca
     6781 acttctatgg tcgtccttct tcgaacactt ggtatcccag ctcgctggac aaaaggtttt
     6841 acaaatgggc tcagaacagg cacagataat caagggaatg aggaatacag cattctcaat
     6901 agtcatgctc attcttggcc agaggtatac tttcctggat ttggctggct tccatttgaa
     6961 ccaacccctt catttgccaa tccaatgggt tcacaaccag ttgctgaaac aaccgaaacc
     7021 gtccagcctg ctatcgaaca accaaatgaa agcgaaaata tggcagaaac tatttccagt
     7081 gagagatctg aggtcattcc atcagaagaa acaacactca caccagctta taaaatcgtc
     7141 ttattcagcc ttggagtgtg tttatccgtc ttgttgttct ttctattgtt attcagaaaa
     7201 tctctgcgct ggacaagtta tcttatctat cttcattttt ttgcttctac taatacgatt
     7261 gcatatcaaa aacttcttaa ccctttggaa acattggtac cgcgcaaacc cgcagaatct
     7321 ttgcaggaat atagcctgcg aatagaacaa gaattcaagc tagatctgcg tttcattcga
     7381 ttaactactc aatatgaagc agaaatatat ggacgacaag cagatccggt aaaattcgat
     7441 aaaaaaacct atttagaaat aacgcgtata ataagaaaaa cggtatacaa aaaaattctg
     7501 acaaaaggga gccgcttcaa aaaataagat aaaatttctg gagaatctac ttctggaatg
     7561 ccgagaacca gctgattgag tatcgattac tcgaaaaaaa ggttgtgaca cgttttttgt
     7621 cacaaccttt tttcattatt atttgatttt caatgtcaaa gcatttagag ctacaactac
     7681 cgtacttaac gacatcaaga cagcaccaac tgctggactt aatatgatac caattggcgc
     7741 aagaattccg gctgctagcg gaattgcaag aatattgtaa cctgcccccc accagaggtt
     7801 ctggatcatt ttctttcgcg tttgcttggc tagatcaaga aaatgcaaaa tatcttttgg
     7861 atcgctgttt gtcaatacga catccgcaga atcgattgcg atatctgtcc ctgcaccgat
     7921 tgccatcccg attgaagcac gggctaaact tggcgcatca ttgatcccat cacccaccat
     7981 gattactttt ttcccttggt caaggtattt ctggacaaca gcttctttat catctggtaa
     8041 taaaccgcca taatattctt cgatccctaa ataatctgct actgcttggg cagcgttttt
     8101 attgtcacca gtaagcatga caggagtgat ttttctttct ttcaatgttc ggataaattc
     8161 ttttgcttct gtttttacta cgtcacctaa agcaacgatt gcagctagat gattttctaa
     8221 aatcaggaag ctgatggtat ttccttgttc ttggtaaggt ttcaataatg cttgttctac
     8281 gtttaatccg agacgctcaa cttctttttc attaactatt ttcacttgtt gttttttgac
     8341 tgttgcctct agcccgactc ctgaaagatt ttttagattt tcagcttgat aaggcttgat
     8401 ttcatgttct ttcaaataat tcataatgcc aacagccaat ggatgattgg cattcgcttc
     8461 caaggctcct agatattgaa ggatttcatt tttattgaac gcatcatcaa gtacttcgac
     8521 acccgttaca gtaaattttc cttctgttag tgttcctgtt ttgtccagca tgatcacatc
     8581 tagatcattg gcttgctcca gtgcattgcg gtttttcaaa agtaatccgt ttttagctgc
     8641 tattgatgtg gagcgagcaa caactaatgg gatagctaaa cctagagcat gaggacaggc
     8701 gatgataaag actgttacca tacgttctaa tgcattagga agatctgtca agaacaacca
     8761 ggcgatgaat gccaggatac ccacaattaa tgcgacataa aacagccatt ttgccacttt
     8821 gtcagataat gactctagct tggatttttc tccttgtgct tttcgcacca tttccataac
     8881 cttcgctaga taaccatttt caccagttcc agttacttcg atttcgatcg ttccatcccc
     8941 attgatagaa ccgccaatca ctttgtcatt ggcttgtttt ttgattcctc ttgattcgcc
     9001 agtcacagct gattcgtcaa caattgtttc acctttcaag attacaccat ctgttggcat
     9061 tttatcacca gaccggacga tcaaacgatc accttcgttt acttctttca atgaaactgt
     9121 ctcttctttt ccatctttcg tcaagcgttt cactgattct ggcagtaatt ctgctagttt
     9181 ttgtaaagca ttgctggcat tcgacacagc attcatttca atccagtgac ctagtagcat
     9241 aataacgatc aaagtagcca attcccagaa gaaatccatg acgtgtgtat gtggattgag
     9301 cagatttgcg ataaacgaat agacactata aatatatgca actgtaatcc ccatcgcgat
     9361 cagtgtcatc atagcaggac ttttttgttt caattccatc ttagctccac ttaaaaatgg
     9421 ctgaccgcca tagataaata gtatagttgc taagatcaat acgagccaat cagaaccagg
     9481 aaacgttact tgaaatggaa attccattcc catcattggt gaaaaaagaa tgattgggat
     9541 agctaggata agcgatagcc aaaatttttg tttgaaattc cccatgtgca tggaatgatc
     9601 catgccgccg tgttcgtgat ccatgtgcat cgaatggtcc atttgtttgc tgtttgctat
     9661 atctccatgg ttatgtccca tgtgcatgga atgatccatc tgactatggt catgtttcat
     9721 ctccatctgt ttttcatgag attctgtact ttctttgttt actacttctt ttttatgctc
     9781 atgttttttc tccatatcgt tcccattcat ttttactcca tcccttctct tactttttca
     9841 tgtgtcgatt taagctcagg gagttcaaca atacactgac ggaactaaac gccatcgctc
     9901 ctcctgcaat gattggattc aaaaagccta gtgcggcaaa aggaatacca atcgtattat
     9961 agacaaaagc ccagaaaaga ttttgtttga tttttctcaa tgtcaaccta gacaactcga
    10021 ttgtttgtgc aatggatgcc aaactgctat tcatcagcgt cacatcggct gtttccatcg
    10081 caatgtccgt tccacttccc attgccatac cgacatcagc taatgctaag gccggtgcat
    10141 cattgattcc atcgcctgcc ataccgactt ttttgccatc tttctgaagt ttttctacgt
    10201 aggctgcttt atcctctgga agtacttcag caaagatatg ctttggatca ataccaactt
    10261 gtttaccaat cgtttcggct gccagtttgt tgtctcctgt cagcataaaa acatctaatc
    10321 ctttattctg gagctgcttg attgcttgtt tggcttctag tttgatttga tcagccacgg
    10381 caatccaccc gatcacttgc ttttcatcag ctaaaaacat gactgttttt ccctgctgtt
    10441 ctaattctaa cgcgtgttct tggtattcat caaatgatag gttcaattca attaatcgtt
    10501 tacgtgtacc cgcaaaatag cgtactccat taatcgtacc gctgatccca gcacctgggt
    10561 gggctgtaaa atcaatcata tcgtatgttg ctgcttcttg cagttttccg tattccacga
    10621 ttgctttacc tagtggatgt tcagaagctt gttctaaagt ataaaaaata gataggactt
    10681 cttttgaacc gactagatct gtaactttgg gctttccttc tgtaatcgtt ccagttttat
    10741 ccaaaacaat gctgtctaag tgagcagctg cttctaatgc ttctccgcct ttaattaaaa
    10801 ttccatttcg tgcaccaaca cccgttccta ccataatagc tgttggggtc gctaaaccta
    10861 atgcacatgg acacgcaatg actaatacag agacactatg gatcaatgcg agttgccaat
    10921 caccagtgat caaacctgta gctatgagcg tgataaacgc gatgactaat acgatcggaa
    10981 caaaaatccc tgagatccga tctgcgattt tttggattgg ggctttgctc ccttgagcat
    11041 cttccaccat ttgaatgatt tgcgctaata ctgtctgctt gccgacttgt gtgacttcca
    11101 tgtgtagcag accattcgta ttgattgtcc cgccaaaaag cgtgtcatcc ggattctttt
    11161 ctacaggaag attttcacca gtaagcatac tttcatctat cgcactgctt cctgaaataa
    11221 ttcgaccatc tgctggaatc tgctcaccag gacggatgac tagttgatca ccaacgacta
    11281 cttcttcaat cgctaacgtt tcttctttcc cgtttcttat cacttgggcg gtttttgttt
    11341 gaagagacat caattgtttg attgcattac ctgttttcgt ttttgctgta tgttccaaat
    11401 atttgccgag caaaatcagc gtgatgatca tactgctgct ttcaaaataa agatcttggg
    11461 ggtgtccacg gaaaaatccg ttgtaaatgc tcaatgcaaa ggcagccgat gttccaattg
    11521 ctaccaatac atccatattt ggtgctttcg tcttgattgc atggtacgca cctttataaa
    11581 aacgagcacc gacataaaat tggacaggag cagacagcac taactggacg attggaaaat
    11641 gaaaaaagtg aacaatcgcc gcatggcttc ctaacatcat agcaatcatt gacaacatca
    11701 acggcaaagt caaaaccgta ctcaatataa gatcgaatag catttttttc aaatacgctt
    11761 gcttttcttc tgcaattttt tgtttgtggg cttcatcgta caaaattgca ccataaccga
    11821 tgttttctac gctttggatt aatttttcag tggtcgtccc ttcatatttg acacttgctt
    11881 tttcagtggc caagttgact gtggcattga ccacaccagg ttgtccgttc agctcttttt
    11941 cgatcctagc agaacaattt gcacaagtca tacctgtaat gacgaacgta tccattttgg
    12001 tttctgctgt tgccatcaaa tcacctctgc tttataaccc aatccattga ttgcttgaca
    12061 aatcttttcg gattgaactt ttgtttcatc aaacttgacc gtaccgtttg cttttttcaa
    12121 atttactttc actttttgca cgccgtcaag tgcgcttact gtttcttcta ctcttgctgc
    12181 acaatgattg cagctcatac cttcgattga aaattgttgt ttcatttttt cttcctccta
    12241 ttgatgttgt ttacattcac attgacctgg gatacagtta cattcgatcg tatctactgg
    12301 ttgcttttgt tccagttgtt tcaaaatctc attgatatct tcttgagtca aagtagcttc
    12361 ttctactaga tccgcaatcg tctctccaat cctttttgca cagatatggg aaaaaagatt
    12421 ttccgtcgca ctgcgaactg tttccatctc cgagacagct ggatgataaa tgaatttttt
    12481 tcctttttgt tctgtccaga tcacttcttt ttttaccaaa cgtcccagca aagttttgat
    12541 cgttgctggt ttccaatcca tcgaatcagc taagatctgc gtgatttctt gggctgtcgt
    12601 ctgtccaagt gtccaaataa cacgcatgat ctcccactct gaatcactga tctttactgg
    12661 cgttttgact ttttccattt atttcacacc tttcgtttac aaatgtaatc attagcttat
    12721 gaatacagat tacgcttgta aacaaaaact gtcaattatt tttgctctct attttcaatt
    12781 tagttaaaaa ttcttgtttc tctcgtcttg ttttaaggat gatcagttta tttcttgcat
    12841 tagctacttg gatcagctgc tgtatttttg gttcattgtt tccttcaaaa tgccagataa
    12901 aagataaaaa ttctaagtat ttcctgttta gtttctccga aaatgattca ggcatatctg
    12961 gccgagattt tttgtttatc tttcttttta gagaacgcca aactactcgg tagatggcga
    13021 gataacgcgg cactttaagc caaatgatct tatctgcttc tttaatcctt tcctctaacg
    13081 tagaagtata atttccttca acgatccatg acggattaga tactataaac gattgctgtt
    13141 cttgcaaaaa ccactttttt gcctgaatgc ggaaagccgg tgatttcttt gatcttcctt
    13201 gcgagggacg attttcccga cccactttgt ccaatcacga tgatcttcat tcttctccct
    13261 cacttctctt tggaatttgg ttcgatcata ccacttataa cgccgcaaaa aaacaaggga
    13321 ctgtgacaaa agtcgtgtca aagtcccttg tttcgaataa atggtgttca atcagctgac
    13381 cttcagcatt agctcaaaaa acggcaaaat atgaggagct atttttgttt ggctgttctt
    13441 attacttgat gcaggacagc tttttcacaa cctctgattc acggtgttcc agaagtagct
    13501 tctgtcacaa cctcttgtaa caagtatttc tattttaagg ctgaaccact gtttctggtt
    13561 cagtaatttc tttgtttttg tctcgacgag caattgctgg caagatcata cctaataaga
    13621 tcaagacaat tggtgtgatg atattcgtga ataattggaa ccaccatgct ttaggatcag
    13681 cagtataatc tacttttggc accattccta ggatacatgc aaacgcagtg aaggcaaagc
    13741 accaagcacc aaagataaaa ccgatttttg gatttttaac aaatttatat tcagaattga
    13801 atttcttata agctttattc aacatcatat atgccaagaa cacccatagg taacgcattg
    13861 gcatgacaac tgagttcaag ttcgtcaacc attttactaa ttcattcata tctttgatac
    13921 caaagatcgg caacaaaata atgattccta ctagtatacc tgtcagaaga taaccattca
    13981 ccaatgtacc tttacgatta cgtttacgta gccagtttgg tacaaattct ggatccgcat
    14041 ctgctaacaa gatttttagt ggtgcatcaa ttgaaaaggc taatgcggag atttgtccaa
    14101 tcatgtttgt caatgcgtaa atgatcatca atgagttacc gataccatag taattaccta
    14161 aaatttgaaa agcactgtat gccccatttg ccatcaagtc tttagggata ttatcgctag
    14221 caaacagcat acccattgcg actgagccta atacagcaca aatccctacc attgctgcta
    14281 agaagatcat ccctttagga aattcctttg caggatttct tgtagagttg acataagggg
    14341 atattttttc cgcccctcca actgcaaata ctagcatcga aacggttgtg aaataactga
    14401 aatcaaatgt tggaatatat gttttcactt gattcatatg tggtgtggca aattccatcg
    14461 ttggattgat gaacggtgcc gttacagcta aaagaataaa taagatcgac atgacgaaca
    14521 tcgctgttcc ggccagtccg ccaatcactt tcaaagtaga aagtcctttt gttgataaat
    14581 ataaaaaggc aagaaatata ataagtgaaa taactgctac catttgtact gacatcgtat
    14641 ttacgatatt tccatttcct tggactgccc aaccaccagc aatcagaatt gcttgaggct
    14701 tttgtgccaa ataaggaata tggacgaccc agtaagtcca agcggcatag tatgccagtc
    14761 gtttattaga tgtcgtattt tcgacccatg aactgacgcc tcctttacta tccttgaagg
    14821 ctgatcctaa ttgtccaacg atcaatgcgt atggaataaa ataaatggcc aaaatcaata
    14881 gccaagaggt gacaactgat actccttgct gagcatagtt attaactaca ttccccatgc
    14941 cccaaaccat gttaaatgca attaacgcaa cagtaaacca gcgtaattgt ttgttgttca
    15001 ttcgttctct cctttatgat aaaaatttgt tatccagatg taatcatatt caatttttaa
    15061 caaacttaca agacagtgtt attttatgtg tgtattattt tatttaatac acatcgtttt
    15121 ttcatatttt ttccatcaaa aaaataagtt attttctttt atttcattga ccttattgaa
    15181 tttcaagcta ttttgaattt tctgcaaaat taaaaacatt agttatttta taatcctatc
    15241 tattttcttt acatttttca ttaagaaaag gatataaaaa aaagaggacg ttcattcgtc
    15301 ctcttcattg atcaacgttt attttttaca tgcaagcttc gcatggcatt caaaacagct
    15361 aatactgtca ccccaacatc tgcaaatact gcatctccca ttgatgcaaa accaaaagca
    15421 cctaaggaaa gtaccaaaat ttttatccca atagcaaaga caatgttttg tttgacgatt
    15481 ttcaatgttt tctttgctaa atggattgct tctgcaatct tcgatggttg atcattcatg
    15541 atgactacat ctgctgcttc aattgcagca tcgcttccca atccacccat tgcgattcct
    15601 acatctgctc ttgctagcac cggtgcatcg ttgattccat ctccgacaaa tgcggtcttt
    15661 ttattgttac tttgtaagat ttcctctaac cgctgcactt tatcttctgg aagcaattcg
    15721 ctatatatct tgtccacgcc tacttgcttc ccgatatgat cagcaatttt tttcgagtcg
    15781 cccgtcaaca tgactgtgtt tttcacacca gcctgtttta attgaaccaa tgcgtctacg
    15841 gcatcttctt tcaaggtatc tgcaataaca agataaccac tgtaactttg atcgattgct
    15901 agataaagca atgtaccgat ttcttgactt gaaggaaaag agatttcaaa tctttccatc
    15961 aatttgtgat tacctactaa tacttgtttg ccatcaactt cagctaagat cccttcacct
    16021 gcaaattcct ggatattagc tgttggtaaa agcggacctg cgtagccttc taaaacagat
    16081 tgagcaatcg gatgtgtaga aaactgttct gcactcgcaa tatattgcat gaatatcttt
    16141 ggatcgattt ttgtatcgat cgtctggatc gaaaagtcgc ctttcgttaa tgtgccggtt
    16201 ttatcgaata ccactgtctc ggtttgcgca agcaactcca agtaatttga accttttact
    16261 aatacaccga ttttacttgc tcctccgata ccgccaaaga aactaagtgg cacggaaatc
    16321 actaatgcac atggacatga gatcactaaa aatgtcaatg ctcgatagat ccattcagaa
    16381 aacgccgctc cagaaacaac aagtggaggt aaaacagcta ataaaacggc caatcctaca
    16441 acgactggtg tgtaatagcg cgcgaattta gtaatgaaat tttcagctgg tgctttttta
    16501 ctgctggcat tttctactaa ttctaataat ttgctgatag tagaattttc ataagatttt
    16561 tctacttgaa ctaaaaatgg tttattttta ttgataaatc cgcttaatac tgtttcacca
    16621 acatatacag atttagggac tgattctcct gtaagtgctg acgtatcgac taatgattct
    16681 ccttcgatga tcgttccatc taacggtaca cgttcaccag gcttcacaag tacaaactga
    16741 ccgatcagca cgtcttcagg gttcacggtc atcaatcctt ccgccgtctg cacgtgagct
    16801 gtttctggac ggatcgccat caattcgcga atcgattttc ttgaatgact gactgccaat
    16861 ccttggaaat actcgccgat ttgataaaat agcatcactg ctacggcttc tggatattca
    16921 ccgatcaaca tagcgccaat cgtagcaaca gacataagaa aattctcatc aaagattttt
    16981 cctttgacga tattcatcac tgccttcttc actacatcat atcctatcag cagataaatc
    17041 aataaatacc ctatcaagcg aattagttca ttaaaatcaa ataatagcaa taaagcgata
    17101 cctgcaaggg aaaataaaat ccggaacagc gtatttctct ctttttcatt tccatgttca
    17161 gatacttttt ccttatctat ttctgttact ttgacatctg gttctaattc cttcactact
    17221 ttttttgttt cttgttcaat cgtagccaaa tgatcttcct ccgcgtcaat cgttagtttt
    17281 ccagaagtga agtttaccgt tgcttccttg actccattga ttttctgaac tcctttttcg
    17341 attttcattg cacagttcgc acaatctaaa ccttctaatc tgtaactttt cattttaatg
    17401 acctcctcgc ctcaacatat gaatatctct tcataactaa ttataaatga atggttattc
    17461 atatgtcaag gtatccgaaa gaaaaaatga aaaacatctg catcggaaaa tttcttccat
    17521 acagatgttt tggcttattc tttctttttc tcttcgatca ttgccactgc tcttatccaa
    17581 cccgcacttg ccttcttgat cttgacagga tagcaactaa gtttgaagcc tgttgctggc
    17641 aatttgtcta aattcattaa tttttcgata ggacaatatc ttttttcaat ccctgcaaaa
    17701 tgtccttccc agatatacga agctccacta cgctttcaaa aatatagaaa gagttcttta
    17761 atgaaaatta tttcaacttt ccataaacag taaccccata taaataaaag acgtaaaagt
    17821 cacatggaag aacttataca ggaaaacgca tagagtcagg tgaaatacat gtatcccacc
    17881 tgactctatg ctttatctaa cctttattat tctcttttat acaatttctg caccgaatgg
    17941 catcaacgcc aatcgagcaa gtttgaaaga ttgcttggca aaaggtatcc ctatgatcgt
    18001 gatacataga aggatgccgc tgacgacatg tgcgagagca attggtaggc ctgaaacgat
    18061 cagccagatg atattaacga tcaatgacat gccagactgg tgataaatta cttctttgcc
    18121 aaaaggcgca aaagaaagga aggcgatctt aaaacattgc ataccgattg ggatacctat
    18181 gatcgtgata caccaaagta tccccgctgc taaccagcta agaccaccga ttagtccgcc
    18241 gaatataaac cagatgacat ttcctaaaca actcattgta tttctccttc tattactgtt
    18301 tttgattaaa actgggcaaa aattgatttt attcttcttt ttttagcttc cagcatcaaa
    18361 tattctgcaa attttttttg gatttcttcc gtattttctt ttgttactaa tgtgtgttgg
    18421 attagttttc ctgatggtcg ctgacccacc cgttcttctg actgttgcac gtccgcttgc
    18481 gcaaaaccga taatccgttt ttcttttgtt acagcaacga gcaatagttt attcttatca
    18541 tttaggtcag ctgccgtttt tgcttttacg aatgattctg gatgatcagt aatcagctga
    18601 ttgatttgag gtagatcttc ttttactgcc caccgtattt ccatcatttc tcacttttct
    18661 ttcgctcatt tttatctcca ctaaaaaaat cgactgcggc aattgcatct atccagcttt
    18721 gtgcaatgat ctcatgtccg ccaatcgttg ggtggacccc atcttctctt gtatatttcg
    18781 aaaaaccata agcaatccct aaagcattga atatgccatc taaaggaacc agcaatgcgc
    18841 catattcctt agcaagttta cgaacaattt ggattttagg gtctaagtct ttacgccagc
    18901 catgacggtc gtcggttata ggaagtacaa acggctccat caagatgatg ttgcagattc
    18961 ccgctttttg aagttcattt agtaattttc gataaacgga ttcaaattgc tgagcagatt
    19021 tctcagttcc gaatatagcc tgatcgctag tattatgcca ggtatcattg attccgatca
    19081 aaatcgatac atagtcaggt tgtaaagcta aacaatcttc tcgccagcgt acttgcagat
    19141 ctgcaatttt gtctcctcct atcccacggt tcatgcattc aaattccatc gttggatatt
    19201 gggagattag ttttccggca atcaaaagtg gataaccttt acctagatca gttgcttctg
    19261 ctcggtctct tcctgcgtct gtaatgctgt caccgataaa taatatacgt tttttcattt
    19321 gtcaaaaact cctttttgaa taaaacgatg attttcttcc ttatattatc ccataagccc
    19381 tatcaagacg aaagagaaaa tatatgtata aaaaatgaca ccagcctagt cccttcaaac
    19441 aattgaatga acgctcatca agtgtctagt tttttatgct atttttttct taacgtgctg
    19501 gattcccctt gattccttca ttgtttggtc cagagagatc gatcacacct aagcttccgt
    19561 caatcagacc acttgatgac gccatcagaa catatattta taggctcatc taagacttcc
    19621 agtaaatgtt cctttactaa tatctcttct agattgcttg ggatagattg gccataactt
    19681 tatctggtcc gatgatttca agggtgaatt gaacttacat atggatcatt gcatgctgaa
    19741 aatacggacc tactccatct tcgaatgtta ctattagttt gttttcaggt gctagatgct
    19801 gctttatttc aacagcagaa gcatcatcaa atcagatttc catttgaata accttctttg
    19861 tatatgtttg attgattgcg atttcgttat agaaccttgt gaatcaaata gcaatttctt
    19921 ttgctttctt ctcttatctg ctggaaactt cctttatccc gtatttaaaa aaaaagaact
    19981 gcgagagttc gcagttcttt tttttaaata aacgttgctc tccggccaac tccttggtca
    20041 caagtcacaa tagtcatcaa acatgaaaaa atgttttttt gaaattactc cttgtgactc
    20101 ggagctgaac ggcagacgca caacctcttg atataaacgg tgttcaatca gctgaccctc
    20161 gatattagct caaaaaacag taaaatatga gaagctattt ttccgttttg tttctctaat
    20221 actcaggtca aggcactgat ttcacaacct cttatggata aattcggttc aacaaacgtg
    20281 ggaatggaat agcttcacgc acatggtcga taccagcaat ccaagtgact gttcgttcaa
    20341 ggcctagacc aaaaccagag tgcggaacag aaccgtattt acgtagatca agataccatg
    20401 aatattcttt ttcatccaaa ccgtgattac ggatttgttc tagcaagaaa tcatagtctg
    20461 ttgcccgttc gcttccacca ataatttcac cgtaaccttc tggtgcgatc atatctgcac
    20521 agatcaccac gtcttctctt gttggatgtg gtttcatgta gaatggcttg atggcttttg
    20581 gatagtttag gataaagaca ggctgttcga atgaattcgc aatgaacgtt tcatgaggtg
    20641 aaccaaagtc ttcgccccat tcgatgtcat caaatccatt tttcttcagt aattcgatcg
    20701 catcatcata agtaatacga gggaatggca attttgtata agtttttaag acttctttgt
    20761 ctcgtcctag tacatctaat gcgtattcgc aatgatctaa aacattttgt actaagaatg
    20821 ctacatattg ttcctgtact tctaaacttt cttcttgatg agtgaaagcc atttctggtt
    20881 cgatcatcca gaattcgatc aaatgacgtc gtgtttttga tttttcagca cggaaagtcg
    20941 gaccgaatga aaagactttt ccgaaagcca tagcagcagc ttccatgtat aattggccac
    21001 tttgagataa ataagctttt tgatcaaagt aatttgtttc gaatagatcc gtagtgcctt
    21061 ctggtgcaga accagtcaag atcggcggat cgattttgac aaaatgatgt tgattaaaga
    21121 attcatacgt tgcacgaatg atttcatttc tgatctgcat aatcgcatgt tgtcttgaag
    21181 aacgcagcca taaatgacga tgatccatca agaagtctgt tccgtgttct tttggtgtga
    21241 tcggataatc gtggctctcg cttaccactt ccacatttgt aattccgatt tcataaccaa
    21301 attttgaacg actgtcttca cggatttctc cagttactaa cacggatgtt tcttggtgta
    21361 gatttttagc tgtttggaaa acttcttccg gcacttcgct ttttacaact acaccttgga
    21421 aaaaggctgt tccgtctctt aattgcaaaa atgcgatttt tccgcttgat cgcttatttg
    21481 cgacccaagc gccgattgtt actgtttctc ccacatggtt tttcgaatcg atgatttgaa
    21541 tttgttccac gtatgactct ccttttatat tccttgaatt tcttcaggat taattttgac
    21601 ttttactctc aataaattgc ttgattctgc gtaccgcttc ttctaacgta gcgcgatcag
    21661 tggcataact catgcggaca ttttctggtg ccccaaagcc ttcgccagtc acaagcgcta
    21721 cccctgtttc ttctaaaaga tcttctaccc attgtgtgac attttcatat ttacacatgc
    21781 gtagcgtttc ttttacatta ggaaaaagat aaaaagctcc ttgtggcttt tcaagagaaa
    21841 cacccggcag ttctgccaca agcggatata acgtattcag gcgttcttca aaggcgttcc
    21901 gcatttcttc cactgtatct tgctcgcctt tcaatgcttc aacagctgca tattgactga
    21961 ctgctgtcgg attactggtt gactgggaag caacagcaat cattccattg atgatctcag
    22021 aattccccac cgcataacca atgcgccagc ctgtcatcgc atatgtttta gacacgccat
    22081 tgatgatgat ggtctgattt ttgattgctt ctgaaatcgt tgcaattgga gtaaactcat
    22141 tgccattata aactaaacgg ccataaatat catcagcaac tatcaaaata tcatttttta
    22201 ctgcccattc accgattgcc tgcagttcat tcttgctata aatcattcca gtcggattcg
    22261 atggagaatt gatgattacg gcttttgttt tttctgaacg tgcttgttct agctgctcaa
    22321 cagtaacttt ccagctattg gattcttccc ctttcacaaa gacaggaagt ccttctgcca
    22381 gttttacttg ctcaccgtaa ctgacccagt aaggcacagg aatgatgact tcatcttgcg
    22441 ggtcaaggat cgtttggaaa agggtataca aagcaaattt cgctccgtct gttacgactg
    22501 tttgtgaagg ttcatagaca agtccatagt ttttcttcag ataatcaacg attgcctgtc
    22561 gaagctctgg gattcctgct gttggtgtat aatagctagc tttcccattg cggattgctt
    22621 cgattgctgc ttcttggatg ttttctggtg tcgcaaaatc cggttctccc acagtcaaac
    22681 tcagcacatc tctgcctttt gcttttaatg ccttggcctt tgccgcagct gccaaagtga
    22741 cagacggttc taatcgtttt gttctttttg ataactccat cccattcatt cctttacctt
    22801 ttaaattccc ttgattgtct tgacttcatc tccagattca aagccaatca ggtaataatt
    22861 cagtttccca tttgtttctt ttcctactac ttcccacaca ggctgatcat catacatccc
    22921 taaagcagct ttttcgattt ggatttcagg atgggccgca gcaattgtct gttttgcttc
    22981 gttttcagtc agtccctttg tttgatccaa tacttttact ttttcaccag atttaggaat
    23041 gataacggcg atttcttttc ctttatcatt cgtacccgtc aatgaaaagt acgttttctt
    23101 tcgagtaaac cagtaaaaat gatcggcttc ctcgagattt gcatatttct ttgcgatttc
    23161 tactgcttct tttttcgcct ggaccgccgg acgactggaa cgcaagtaaa aaatcgagct
    23221 gatcacgatg accgctaata aaaatacgat caagccaagc aacacgcgat taatcgtttt
    23281 ttcctttgtc tgttttttct tcaagggtcg tcgctccttt atcttcattc cttttcatta
    23341 tagcagattt taatgatgaa aaaagacggc acctttaact tccttcttca tttttcttta
    23401 agaattcatg gatatcctga tagatttcat ctatagctag ctcttttatc ggtacttttt
    23461 tcggtaatgc atggatgatt cgtgcaccat attttgcagt gactaatctc cgatccaaaa
    23521 taagtaatac gccacgatct gtctccgtcc ttatcaaacg acctaatgct tggcgtaact
    23581 tcaaagctgc tttaggcacg gattcctgat aaaaaggatt gatcccctct gattccagat
    23641 aagcattcct agctttgacc atcggacgta acgggttgtc gaagggtaaa cgtgtgacta
    23701 caacaatctg caaagcatct ccaggtaaat ctacgccttc ccaaaagctg tcagcaccaa
    23761 ataagacgct tttttctgac aaaatgaagc gtttcaaaag tttttcccga ctcccgccaa
    23821 atccttgagc aagaatttca cgtccttttt ctagcataga caaatgcatt ttctgatata
    23881 ccttttgcaa aacatcatga gaagtgaata aaacaaggat cggccgatct tcttgttcaa
    23941 tcaatcctgt gagtgctgct tctaaataat cgatatatgc ttgattgctg acttgatgga
    24001 ctgcaggtgc ttctttcggc acatagattc ttgcctgttt ctcataatca taaggagaag
    24061 caatcacttt aagaggtgct gcttcaatgc caagtcgttt agagagatac tgtcgattag
    24121 gtccgatctt cagtgttcct ccaatataaa gaatatggtc atatcgagga taccacacag
    24181 ttgacggcaa catagctgct tgaaaatcaa ggagatgaaa aatcatctgc tgtgctgtat
    24241 tcccgggaac gaaccaatgg atcaaatgat ctcgccagtc ttctagccat tggtccatca
    24301 gcaagtattg cttgtctaat tgatcaaata agctatataa acttgctaaa tcgatccgca
    24361 tctggttggt ccaactttct tggataatct caaaagtcaa gcgcatctgt gattgtagtt
    24421 gttggatctc ttgataaaaa agttgcagat gatccattgc tgttccagat tccagtggca
    24481 gctgatccaa catttctttt gtcatcatga cttcttctgg atattgtccg ttaggttgct
    24541 tgctgcttac taagttcatt aatccctctg ccacgcaaat ctgcgcttcg catagtgcag
    24601 agagttcttc tcgataaatc gacaaccagc gcaaggcttc atgttccttc ggcaaataac
    24661 tttgtatgcg gtcaaataac tgattcggct cttccagttg ctgaatttgt ttatggaagt
    24721 agttaatact tagtcgtttg tcatttactt tttcagcaat ttccggcaaa tgatgcactt
    24781 catcaatcaa caaataagga ctagctggaa gcaatggttc ttttcgctgt gtttcttgta
    24841 ctaaaaatgc atggttgact atcaaaaaat tgctttgttt gattttcttt tgcaagaaac
    24901 gcacaaagtc ttgtttgtaa aatgactgat tccttgctaa aaacccagtg cctctgtgcg
    24961 cgacatctgc aaagaatgca tggttcagat tggttaaatg taattcatcc aaatcaccgg
    25021 tcttcgtttg tgttagccaa actaagacag ccatttggta caaagcatat tgtttttgtc
    25081 ccggcggttc cacaagtgta cctttaaaac gctgtaaatc aatatagtgg cgatagcttt
    25141 tgataatcac aggttgtatc ggctgatcta gcaattgatt cagtaatgga atgtcttttt
    25201 ccatgatctg ctgctgcaaa acaagcgaaa cggtgctgat gacagctggt ttttcaggtg
    25261 tagccaaata actcaaaggc aacagataac caatcgtttt gcccattcct gtagctgctt
    25321 cgatcatcaa atcttttgat tcatcttttg tgaaatggtc gtagacagca ttcatcagcc
    25381 tgttctgttc ttttcgatac atcagtgttt taccaaacat tttttctttt gcttgttttt
    25441 ttcttggata tgtccgctcg ccataatgga cggatgtgaa tagctccact tcttttttct
    25501 gcaaagctag cccatcgatg atttctaatg agagatcaag gggctccggg ttttctttca
    25561 tttcttcaag cacgtggtaa atgaaacgac tcgtatccat tcctgtatgg ctacttaatc
    25621 gtgcaatctg ctctaatgtg atgatcggca atttcctcat tcgttcttcg atcagtaaaa
    25681 gaagctgacc tgtcacttcg gcatcactat ccgcctgatg aggattctca tgaataaagc
    25741 caaggctttc tgacaaatca gctaagcgaa aacttggttc cgtcggcaag aaaatttgtg
    25801 caagttctac ggtatcgatg cccggtatct tcaaactagg aagtccgcat cgcatgagtt
    25861 catggttcaa aaaattataa tcaaaataaa tattatgcgc gacaaatatg gtgtctgcca
    25921 aaagattgta aatcgtttgg gcgacatctt caaaataagg cgctttttgg acgcgttgat
    25981 tcgtgatccc tgttaaatgc tggatttgtt ttgaaatctt ccgccctgga tgaatatcga
    26041 tcgaaaaacg tgaagtgatc cgaccagctt caatcatgac gcagccaaat tggatgatcc
    26101 gatcttcttt tggattcgtc ccagtcgtct cgatatcaac gactgcataa gttcttggtt
    26161 tcaaaatatc ttcctcttct cctgcctcta atgttctttt gaaattatac tacaaagtgc
    26221 agaaaaatac tctcatcgct ttttaaggaa aaaaagacaa aaaagcccag acacataaat
    26281 gtctggactt ttatttttaa tataggtaaa ttaagcttta gtaacgtttg ttgcttgagg
    26341 tccgcgttga ccttcttcaa cgtcgaaagt tactgcttgg ccttcttcta aagttttgaa
    26401 tccgtcgcct tggatagctg agaaatgaac gaatacatcg tttccgtttt ctgcagtgat
    26461 gaatccaaat cctttttctg agttaaacca ttttactgta cctgtttcca taataaaaat
    26521 cctccttgtg tatcaacaca tattttgcaa ttacttgaat ggtgtatatc tggaagatgt
    26581 ttatttgaaa caagctacca cagattaccg aacaaaaact acattcttac tatatcatac
    26641 cgtttacaaa attgccagag tttttcctta tttttttgta tttattctct tatttagaag
    26701 atatatttgc aaataaacga acgttttctc gaaatcaccc ctataagatt cagaaaattt
    26761 ctatttgcta ttttcacttt tgcaaaaaat ttcaatttat tatctgaaaa aggatgaacg
    26821 aactttggaa ttctctaatg atccacatca ttttttaact gataaaaatt agtatgcgca
    26881 tctatccccc tctcaactgc aaggttcgtt tttttgagaa aagtatcttc ccctcgcaaa
    26941 gccaatggga ctaaagcaca aaaataatga gcgagagaca aaagctagct tttgtctctc
    27001 gctcaagatg aatccaacgg ctgaaacaga ggaaactcct tctctcggag ctatacactt
    27061 ctatcaacgc ctgaccattc atctactttg gtagttttca ctcaataacg taagatcgca
    27121 actaaactct tttcgtctgg tgcatctttt tgttcaagga taaagacctg accaccgttc
    27181 gtgatcacat tatctgctag ttcattcagc acctgacggc ggtcatattc tgtttctgga
    27241 tcttcgccaa aaccatctgc taaattagat gtagcaatga ataaatggga tattttccct
    27301 tcttttgctg ccggtataat atcaacgagt tgatcaatga actttttatc aagcaactta
    27361 ttgtagtcac ttgcttcttt gacagctaat tcttgcgata gtttttttgc gcctgcttca
    27421 atttctttta atgaaagttg tgcaggtgaa gctgagatcg caacatcgga acgataataa
    27481 gggttcttcg ccactttttt gaacatcgtc tgattttctg gtaatgcaaa taagaagata
    27541 ggccatttct ctggattttc aaatgtatct ttcaaatagt catcgactgc ttgataataa
    27601 ttgacccaat caatttcgac ttcttcatct ttaggattga ctccatggaa cgtcgtacct
    27661 tcactaccat ttgatgcgta gttcaacccg cctcctgtta gttcagatcc taaagcagtg
    27721 acgacatctc ttggtgcatc ttcaggcaaa gccacttctt tgacttgttt attcgttact
    27781 tcgtacaatt tcatcgaatc acgattcaat cctaataaat agtatgaata gttgaaatgt
    27841 ccatttttga tgatcgctaa taaataaggt agatcactca cataatactg gtcatctaca
    27901 ggaataccca aacggtggat aaacacttgt tctggtgata ctatgacagc tacactcgtt
    27961 gtggcactac gccagaaaga cggatcgtcc aacaaactag taattttttc ttggaatgga
    28021 gaccaatcat gctcagagta tttcttttca aagcgctttt tcgcttcctt tgcaaaattc
    28081 ttgaatttga tttgatcttt ttctacgtct tgatgcgcaa catgggtgtt taacataatc
    28141 gtgacaaacg gtcctttggt accttctgaa gtcaattgtg ataataattc tttttctttt
    28201 tttgccataa acgtacccct cctaaaaaaa tcatttcttt gtatctgtaa ataaagtcta
    28261 ccacatccca ttttatcaat acaataaaac gaatccctac cagagaaata cctgatcgat
    28321 ttttagttgt gttgtcctac ttcatatttt tttcatactt tcatcaatta tatgctcatt
    28381 tccttaacag tagcttcaaa ctctcttgct atactataaa aaaacaaaaa agaacgaggt
    28441 gatcttatgc aattattcat gatttttagt ttggtcgcac tctcagcagg cgcccttttt
    28501 gcagcatcac gcctaaccga acccgaagtt cagccagtca aagttcgtgt caatgaaccc
    28561 aaaagataac tttctatgat tcttcattaa taagacttgt tctttataag gtttttttca
    28621 gaaccattga aaccttttgt tctctctgat aatattctaa gttgtaagaa tcaatt
//
LOCUS       DAXWOO010000017        27872 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.017, whole genome shotgun sequence.
ACCESSION   DAXWOO010000017 DAXWOO010000000
VERSION     DAXWOO010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27872
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.017"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(424..1368)
                     /locus_tag="ACGE70_000603"
     CDS             complement(424..1368)
                     /locus_tag="ACGE70_000603"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFF8692259.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1664..2608)
                     /locus_tag="ACGE70_000604"
     CDS             complement(1664..2608)
                     /locus_tag="ACGE70_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFF8692260.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2829..4043
                     /locus_tag="ACGE70_000605"
     CDS             2829..4043
                     /locus_tag="ACGE70_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFF8692261.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASIYGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            4065..6080
                     /locus_tag="ACGE70_000606"
     CDS             4065..6080
                     /locus_tag="ACGE70_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase"
                     /protein_id="HFF8692262.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEETFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6163..7536)
                     /locus_tag="ACGE70_000607"
     CDS             complement(6163..7536)
                     /locus_tag="ACGE70_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFF8692263.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7562..7846)
                     /locus_tag="ACGE70_000608"
     CDS             complement(7562..7846)
                     /locus_tag="ACGE70_000608"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFF8692264.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7848..9863)
                     /locus_tag="ACGE70_000609"
     CDS             complement(7848..9863)
                     /locus_tag="ACGE70_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFF8692265.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELWSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(10042..10347)
                     /locus_tag="ACGE70_000610"
     CDS             complement(10042..10347)
                     /locus_tag="ACGE70_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFF8692266.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10436..11467)
                     /locus_tag="ACGE70_000611"
     CDS             complement(10436..11467)
                     /locus_tag="ACGE70_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFF8692267.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11674..12549
                     /locus_tag="ACGE70_000612"
     CDS             11674..12549
                     /locus_tag="ACGE70_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFF8692268.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12701..13543)
                     /locus_tag="ACGE70_000613"
     CDS             complement(12701..13543)
                     /locus_tag="ACGE70_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFF8692269.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13635..15512)
                     /locus_tag="ACGE70_000614"
     CDS             complement(13635..15512)
                     /locus_tag="ACGE70_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFF8692270.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15509..16930)
                     /locus_tag="ACGE70_000615"
     CDS             complement(15509..16930)
                     /locus_tag="ACGE70_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFF8692271.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16933..20322)
                     /locus_tag="ACGE70_000616"
     CDS             complement(16933..20322)
                     /locus_tag="ACGE70_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFF8692272.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20579..21970
                     /locus_tag="ACGE70_000617"
     CDS             20579..21970
                     /locus_tag="ACGE70_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8692273.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(22043..22690)
                     /locus_tag="ACGE70_000618"
     CDS             complement(22043..22690)
                     /locus_tag="ACGE70_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFF8692274.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22698..23384)
                     /locus_tag="ACGE70_000619"
     CDS             complement(22698..23384)
                     /locus_tag="ACGE70_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8692275.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23390..24463)
                     /locus_tag="ACGE70_000620"
     CDS             complement(23390..24463)
                     /locus_tag="ACGE70_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8692276.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24685..25359
                     /locus_tag="ACGE70_000621"
     CDS             24685..25359
                     /locus_tag="ACGE70_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFF8692277.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25364..26704
                     /locus_tag="ACGE70_000622"
     CDS             25364..26704
                     /locus_tag="ACGE70_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFF8692278.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26853..27839)
                     /locus_tag="ACGE70_000623"
     CDS             complement(26853..27839)
                     /locus_tag="ACGE70_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFF8692279.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8133 a         5268 c         5045 g         9426 t
ORIGIN      
        1 aaaaatgata aacctctctc gaacggtttt ttatcaaaaa tcgtcatttt tccatcaaaa
       61 tctgcttatg caaaattata aaaaaatttt gcagtttttc ttactttttt tgtcgtttaa
      121 ttgaatgata aaaaccagtt tctttattca gttaatcaaa tatgaacgaa ttagatatga
      181 atcattgtat ttgccgttgc ggaaggattt tttaattaac cacagataaa cattgataac
      241 gattcgctta ttgtaatcaa cagattaaat ttcatatgtt catatgtacg gatacatttt
      301 cggaccgatt aattttaacc attctgttta tttgcttacc tattagatat caataaaaaa
      361 ctgcttaaca gctatttcag ctgttaagca gttttaggat ttttagttca tattcattgt
      421 ttcttataat acagtgttca atacttctct taaagcactt gttgatttgc tgaacattgc
      481 tttttcttct tcgttgattg ataattcaac aacttcacga acaccatttt cgtcaacgat
      541 tgatggcaca cctgtaaaga tatctttttc gtcgtattca ccagttaaat aagcagatac
      601 aggcaataca gcttgttcgt tattcaagat agcttttacg atacgtgcag tactcatacc
      661 aataccatag taagttgctt ttttacgatc aatgatctca taagcagcat tacggacttt
      721 atcagcaatc acatctaatt catcttgggc aatacgatgg tctttttcta caatttcaaa
      781 cactggttta ccaccgacag ttgtatgtga ccatgcagca acttctgaat caccatgttc
      841 acctaagata tagccgtgga cgctgcgtgg gtcaactttt aatttcaatg cgatttcttt
      901 acggaaacga gttgtatcca atgttgttcc agtaccgata acacgtgatg ttggcagacc
      961 agattcgttc caagcgatat atgtcaaaat atctactggg tttgaagcaa caacgataat
     1021 accgtcgaat cctgatccca tgatttcttt tacgatttgg cgcatgattg acgcatttgt
     1081 tttaactaaa tctaaacgag attgaccagg tttttggttg ataccagctg tgataacaac
     1141 gatatcagca tctttacatt cttcatagcc acctgaccat acagccacat tttcatcgcc
     1201 ccaagccata ccatctaaaa ggtccaatgc ttccccttct gctttttctt gattcacatc
     1261 gatcaatact aattcattag agattccttg gttgatcatt gcataagcga tacttgttcc
     1321 aacaaaacct gtaccgacga ttactacttt acgacttgtt tttttcatga ataaacagct
     1381 ccttttcata actatttgtt actttcatga aaagtatagt cttgtttatt cattttatca
     1441 acatgttaat tataatagtt gttattataa tttttgatgg aaacgaattc ctgaatacgc
     1501 ataacagata aatgacaaat gtttacacct atatcccctg ataatacaac caaaagttta
     1561 gaactgaaaa taaatccaaa aaaattcgct gcaacgatat aataagacag caatcgcacc
     1621 taaaacaatt ttccttcata tgctttagtg aaagattttc ttcttaatgt ttatttctcc
     1681 attcattggg agtacatgtt gaatacttct tgaataattt gaataaagaa gtcgtttgtt
     1741 tatatccgat acgattagcg atttcttcca acgaaaaact cgtttcttga atcagttcca
     1801 acgcaaagtc gaattttttc tttagaacca gttcttgaaa ggaagcatca aaatacattt
     1861 tcaatttatt actaagatag accggattgt agccaaaatg agctgctgtt tcttttaacg
     1921 tacaagaagt aaaatgctta tcgatataat ctaatactct aagcatctca ttttctgcgc
     1981 atccatctgc tgatgaaaag ctttgttgtt ctaataaatg agtcaattcg atcagtaata
     2041 gtgatgtata gagatttaac gaacggatct tatgactgta tgtatcaaaa taaatcaaca
     2101 gcatattttc aatcaaatgc gtcaaacgac ttgtttttcc gccgcgaaaa tgaatgaatt
     2161 gtgtatgttc ccctttttct tttgatgcat ttatcaaaaa agaagaaagt agattatttg
     2221 tgcttgccaa atgattcaat atctctgtag ataaggtttc ttcttttaat aagatgttga
     2281 ttaaaatatc ctcttgccct gtgtattcga ttctttgagt agcttcttta tcaatgatca
     2341 ccacgtcact ttcttccatc tctatccttt ctccattaat aatttgtaaa catgtaccgc
     2401 tatacatata attgatttca acaaaatcat gtgtatgctc gggaacttcc gcatatcgac
     2461 ggtgtttact gatatgaata tttccctgtt gaaatagatc ccctcggtgg atttttggcg
     2521 gtgtctgaag atggtaagct aagtttaaat cattgatatt cattccttgt tcacgctgca
     2581 gagtttcgat tgattcttcg ttacgcaaaa aaaatgtcta attcagctag ttccaattga
     2641 ctccctcctt tttgtaagcg gtttctttcg cttattagtt cacgctgtac tgctattata
     2701 tgcaaagcta tttttggttt cataaagact ttctatggtt gggacatgaa cttcctgtga
     2761 ttccgctatt gagagaataa ccacttcata ctatattttt gataagcgct tacaaaaatg
     2821 aaaggaacat gcttatgaat aaaaagatga tgaaattttc tctgctgtca gtctctctta
     2881 ttttgacttc tgctggtgca gtcacgtctg caattcccag tatgtcagct acttatgcca
     2941 cgcgttcttt atctgctgtt gaattattaa cgactgctcc ctcttttatg gtcactattt
     3001 ttgttttgat tagtagtttt attgctagga aaatcggaca aaaacaaact gtgatcatcg
     3061 gattagtgat tgcatcgatt tatggtatcc tccccctctt ctcacaaagt tacagtattg
     3121 tcttagcttc aagagctgga ctaggtattg gctttggatt gatcaattct ttggcagtca
     3181 gcatgattgc tgccttttat cacggagatg aacgagcctc tatgattggt tttcaaagtg
     3241 catttcaagg atttggtgca gctttaatga catttgttgc aggacagcta gtacagttcg
     3301 gttggcaata taccttttta gtctacgcca tcagcttacc tattcttgtc ttgtttgttt
     3361 tgtttgttcc tacacctgaa acaactgaac ttcaatcaca tggcacagcc ccaacaagac
     3421 aagtaatcaa aacgaatcct aaaatgatta gttatggtat ttttctcttg ctggtcatcg
     3481 tggtatataa tgctgtatcg attaaattag ccacagtcct gacgacttat tctctaggaa
     3541 acgaaactaa tgcagcgaat atcttaacaa tcatgcaatt ggcaagtatg gctgccggag
     3601 tttgttttgg ctggcttcag acaaaagcac atcgctatat gcttacactt tctttactac
     3661 tgatgggaat cggctttgta ttgattgcca ctgtgataaa cctgtatgtg attgggtttg
     3721 gtgcgatcct aacaggaatt tctttttcgt tattcattcc ttacttattc aaccaagtaa
     3781 gtatccaagc gcccaagtac gcggagagtt tcaatacttc aatcttattg gttggcgcta
     3841 atttaggtac tttcattgca ccatatggtt tgctctttct ctcttttgct tctcttggga
     3901 gtaaaacact tgctgttttc gtcaatggca gtatcttgct gttgattagc gcgcttatta
     3961 gccttctagt agtacacagg acgaatagaa agacagtgat aagagtagat gaagaaataa
     4021 aagaacaatt gacaacaaaa taaatttgat ggaggtcgtg taaaatgaca aatgaaaaac
     4081 ataaagatag atggctttgg tatcctggag actttgagat ccgccatgga ctgctacaaa
     4141 atttccaacg ggaagaacga ggttttgact ggccagcgta ctggtacatg gatgattgcc
     4201 atcggaacgt caagtttaag agatattact ttcttgatca accttccatg tttaaagtga
     4261 cgatccaagg agttgggtat gtagaaatta atggacaaaa acatccatgt ggaaaatggc
     4321 tgacttgtcc agctggtaaa gcaaaaattc gtatctttgt aggacataca tcagggctac
     4381 cagcgatgtt tatcgaggga gacgaagtaa aatcagatat tggctggaca gcaagtaatt
     4441 ttattgaaga atatccagct ggctggagtc cgctttatgt cgatatcgca aaagatccaa
     4501 atcaaatcta ttatcaaaaa gaatgcgtcc atccagtcac tgaaacggag attggcggag
     4561 gaatcttatt cgattttggg cgagcagtga atggctgtgt agaactaata tctctggatg
     4621 accatatcga atctatcact ctatgttatg gagagtccga tgtagaagct atggatatcg
     4681 attattgcta ctataagcaa ataggaatga caagaaaaat gaaagctcgt aaacgcgctt
     4741 ttcgctattt gtttatacca gaggtttcta ctggaatggt tcaacttcaa gtttttcatg
     4801 aatttttatc tctttcgaaa caagctgctt tttcaagcga ttcttcttta ctgaatcaga
     4861 tctggtcagt atcagaagaa accttcaagt tatgtagtga tttgtttttt atcgacggta
     4921 tcaaacgaga tcgctggatt tggtctggag atgcgtatca atcattcatg atcaatcaat
     4981 atcttttttt tgacgaggaa atcaataaac gaacgatatt agcattaaga ggacaaaatg
     5041 aaatcaagca gcatatgaat actatcgttg actattcgat cttgtggttg attggtatcg
     5101 aaaaccacta tatgatgacg aaagactatg aatttttacg catcgtctac cctaagatgc
     5161 aatcattaat gagatacttg atggagcaaa cgaatgaatt agggtttatt tatgggagag
     5221 caaaagattg gatatttatt gattggtctg aaatggataa agaaggaacg cttgctgcag
     5281 aacaagtctt attactgaaa gcctatcatt ccatgatcac ttgcggagat atcttagctg
     5341 aaacatatgg agagaacgaa aattatcata ctcatctcta tcaagaaaaa tatcaactat
     5401 tgaaacaaaa cctatttgac tacttttggg atgaagaact tggcgctttt atcgattgct
     5461 atgaatctgg gagaagaaat gtgacgcgtc atgcgaatat tttcgctgtt ttattcgatt
     5521 ttgctaacaa agaccaacag caatcgatct taaagaatgt cttattgaat gacactatca
     5581 cacagattac tactccatat ttcaaattct atgaacaaga tgctttatgc aaactcggac
     5641 agacgaatat cgtctaccaa acgattcttg attattgggg agggatgtta tctcatgatg
     5701 cggtaacttt ctgggaagaa tacgacccta ctcaaacagg aaatgaaaag tacagtatgt
     5761 atggagatcc ttatggaaaa agcttatgcc atgcatgggg agcaagccca atctatcttt
     5821 taggacgcta ttttatcggt ttgtatccaa caaagccagg ttatgaagaa tttatgatac
     5881 aacctaatct agattcattt gaaacactga attgtacgct acctatcaaa gatggaaaga
     5941 tttcgcttga aaaaacaaaa gataagatcg tgattacttc tgatagaaca ggcggaacag
     6001 tgaaaatgaa tggtaaaagt tatcctcttc tagcaaaaca gccacttgtc ttaacaagct
     6061 attcaaaaat aaatgcataa aacaaaaaac tgcttgcgac atatcagaag actttaaatc
     6121 ttcttctgat attgtccaag caatttttat tttgaagttt gcttaattct gttctttaga
     6181 aacaatgttt gttctttttc cggcaaaaag aaagctagat aatacgacaa gtaaaaagaa
     6241 agcgatgatt ccaactgtca aggctatttg accgtattct cctagtttcc caagtacaag
     6301 tcctggtact agataatcag tatcggcaaa tgtagaagaa gcgattttaa gatctcctaa
     6361 tactggaagg ataaataatg gcagccaact aatcaataag ctattgaaag cagaacctaa
     6421 taccgcccct cttctaccgc cagtcgagtt gccgaagaca ccagaagcag ctccgcagaa
     6481 gaaatgtcct actaccccag gaatgataac tgtagtttgc aagcctatca tagcaaacat
     6541 ggatagtgtt cctacaaaga agctaacaaa aaagccaatt aatacagcat taggtgcata
     6601 tggaaaaacg ataggaacat ctaatgctgg tttagaatcg ggtacgagtt ttttagcaat
     6661 cccttggaat gctggaacga tttctgccaa caccattcga accccttgaa ggacgataac
     6721 aaacccagct gtgaatgttc cagcttggat cagtgcaaag attacagcgt tgtctccatt
     6781 gcttagttct ctttcaacaa atccaggacc agctaacaaa gcaagtgcga catatacgat
     6841 cgacatcaag agcatgatcg atacagtaga atctcgcaaa aagctgaaac ttttaggaat
     6901 ctttatgtcc tctgtagaac ggtctttgtt tccaaaaaac tttccgatcc atccactaga
     6961 agcataaccg atatttccgg tatgccctaa cgcaacttga ttgtttccag tgatgttctt
     7021 cataaacggt tgacaaagag caggcgtaat agttaatagt gttccttcga agaccccgcc
     7081 taataagatc atccacaaat ttgcagtaaa ccccgcgctt actaaaatga ctgctgttag
     7141 acaagaaaca taaagcattg cttgacctgt aagaaaaatg aatttatatc ttgtgaatct
     7201 agcaagtaaa atattcacga tcatccctac tagcatgatc aaagcagtag gtgtaccgta
     7261 ttgttgcaaa gcaatcgcca ctactgcttc attgctagga actactcctt gagtatgcag
     7321 agctgtttgg aacatcgaag caaaaggggt tagtgaatta ctgattaaac cagcaccgcc
     7381 gatcagtact aggaatccga caaatgtttt gatagttcct tggatcactt ctgaaaattt
     7441 cttcttctga agaacgagac caagcagtgc aatcaaagaa acgaggatgg agggctgact
     7501 gacgattgac acaaacaaat tcaacaattt atccattgat agttcctccc ttgaatcatt
     7561 tttattcttt tgacaagatc ttcgttactt tttcttttac ttcttcgctg tcaatgatcg
     7621 attgcaaagg tacgagcttt tcatcaggta tagaagttag cgtatctttc aaagaatgct
     7681 caatgaagaa ataatcagcc atatcgctag atacacttcc aacatccgta tgacttactt
     7741 caaactcgtc cacatctgct ccaagatctg caagtgcttg tttgatgttc atctctacca
     7801 taaaacttgt tcctaatcca ctttgacata ctgcaaaaat tttcatatca ttcgtctcct
     7861 tttcttatga tcgctaatat ttcttctttt gttgatgcgt gtaataaatc ttgaagattt
     7921 tgtttattag aaagtagctt tgttaaattt gccaaagccc gaagatgtgc ctcattatcg
     7981 atagcagcta aacagatgaa caaactaatg ggatgggctg gattatcaag cagatccaca
     8041 ggttgttgaa gtttcagcaa agacatccct acttcgttta cgccatcttc tggacgagca
     8101 tgaggtaatg cgatgtgatc gccaatatgg ataaacgcac catactgctc cacgcgctcg
     8161 atcatagctt tgatataact ttcttctatt ttattttctg ataatagcgg ctgtgcggca
     8221 aatgtgattg cttcttgcca gttttttggt tgatccgata attggatcat ttcttctgtt
     8281 aataattcag atagcatagg acgttgatcc tccttctttt ctaaaagttt gttcatttta
     8341 cgatttaata cttgatagag cttttcttcg tctacgcctt cttttaattc gatataaggc
     8401 agtagcgccg accacaattc ttgggcagaa ggcattgaaa atcctggtat cagccaatct
     8461 ctttgtactt gattgatcag ctgatttttt tccaagtcag tcatgattgg atgcagaaca
     8521 tagagagact ttttcttatc agtaagtgtg agaggtacag tagaaaaaat aatatcgtaa
     8581 gaagtttcgt caacctcgtg taagcggtca gctgaattag tctctgtaaa aaagatcatc
     8641 gggaataatt gctgaagttc actttttaag atcaatgaag agctaatgcc acttggacag
     8701 acgatcacgg ctcttatttt tctatttctt tcttctgcgt ccgcttttct gatctctccg
     8761 ccaaatagta tagtgaaaaa accaatctct tcttcaggga tgctcttccc tattcgtctt
     8821 tctaacggaa gtaatgcttt tcttgtcatt tcgtagatgg aagcatattg atgcttaatt
     8881 tgatcgatca tcacattagg taaataaaaa gaataggaaa tacgaaaata ggcagggacc
     8941 aagtgataaa agacattcat caataattca cgaaacgaag caaattctac ggccatcagg
     9001 aattccatgc gatggatgac ttctgaagaa cattccagca aaaaatcaag tgcaggctcc
     9061 tggacatctc cttcaacgat cgatgctaag caaagagaga aaaatataat ctccgaattt
     9121 tgatgttcgg aatagccaag gccagtcaat acttgtcgac taatgtcagt gactgcaaga
     9181 gaagccaaca gttcagcatc ttttaccagt tccatggatg tttttccaat tcgaatggtt
     9241 gtcaaactca aataataagc taattcatcc attcgatttg gtgcgaatcg aagatctgat
     9301 agtgaaatcg ttttatctag caaatctcta actttggcgt aataatcaat ggaaagggtt
     9361 gcggttaaat acgtgagccc aaatcttcca gcaagtgact gcatcaaacg agcagtgcga
     9421 ttttttgctt ctcttcttaa tattgcttct gaccctagca gcctaaaacc agttttccta
     9481 gtatacgaca gctgacaatc ttccacttct aattcatttc ttatcttttt gatatctgac
     9541 aggactgtcc ctttagaaac ctgaagaaaa tcttgaaaat gaaaaacaga aacagacgtt
     9601 gcagtcgaat aaaccatcaa ataaagaaga tctcttcgct cttgttcggt atacacagct
     9661 tcaaatcgag taatatccaa aaatttttct gcccagtcga ttgctcggaa tttctttata
     9721 cggatacggg tatcatccac atattcggca aaaggagtta attgtttgat cgcctcgctt
     9781 gtatttactt cgatgatctc tgctgctttt tctagactga tttctcttgg tgttttcagg
     9841 agataactaa ttttgtagtt cacagcgctg ttcacctcct acttatattc tagtatggta
     9901 gcgcttttaa atcgaggggt gtttggtaca acttacagtt ggattttttt tacgactgta
     9961 ccgaacgatt tctatcgaat ggatacagaa aaaaagcagg tgaccaaatg tctcctgctt
    10021 ttatgaattg atttcgtccg atcaatgatg atcatgttta ttttttgagg tcagttcttc
    10081 aaatttttct ttgacatctt caaccgcttt ttcgatatct ttttcaaagt gattgggctc
    10141 attaggcttt gtttcctctt ctacagaatt tgaaccagat aaagctgttt ctttattttc
    10201 tgtttcacca gagtttgtgc cagctaatga ttcttctttg tcttctgttt caccagaatt
    10261 agttcctgct aaagccactt cttcttctgt tttaggggcc attgaacctc ttaatcgttt
    10321 gtgtttttca ccgtcataat ttgtcatgtt ggttcctcct agtagttcta tactttttca
    10381 tcgtacaata agacagttta tgcgtcaaaa aacaaacccg attcttacaa atatgtcata
    10441 tttttccttc tgattcttga atgcttttga ttccttcttc caaaattttc atttgtaaga
    10501 ttgtttcttc atctttgatg actttttctg cgccattaac taatgtctcg taaatccctt
    10561 cgtagacacg gctgtaatca ccaacctcgg aaattacttt ttcttcatga taactccctt
    10621 gttcatctat ataggtcaat acaccataat gttcgggcaa atcgatacca aagtcttcat
    10681 gatcaggcat gtaaaacatt ttcagatgtt cttcttgtcg atcttttgtt tgtttcacga
    10741 acatcccttt ttttccgtaa acgacgaaac ttggacgttc tttgatgcgg aaataactgg
    10801 attttatcga aattttcata tttccataga aaaaatccag atcgaagtaa tcattcattc
    10861 gtcctttccc taaaagttga cgtacatcat agctgatatc atctggttcg ccgaaataag
    10921 aaagaacttg atctactgta tgacaagcat gtccatataa atagctgttc gcagtagaaa
    10981 attcatgcgt attttctggt acttccggtc tgaaataatc ataatgcatt tctacttcta
    11041 aaatatcccc taattttccg ctttcgatta ctttttggac tgtcaagaag tcagaatcga
    11101 accgtctgtt ttggtaacat tggataaata gacctttttc ttttgctaat gcaaataact
    11161 cttctgcttc ctttgacgtt tctgcaaaag gtttctctac tagtacattc ttaccatgtt
    11221 cgagtgccat ttttccatat tcataatgaa attgactagg tgtagtgatg acaactaatt
    11281 ggatctcagg atcatcccaa agatcagaca gctgatcagt atattcgatg tcttcgaatg
    11341 ttttccacac tggttcagat ggggaaaaaa tttttttgat attgattttg ttcgtaagtt
    11401 ttcttgaaaa aggcaaatga tagcgatttg tactctttcc attgccaaga taagcgattg
    11461 taagcatgag tggtgcctcc ttttattgat gttttcagta tatcctagaa aagcgcagaa
    11521 agtatttata atagctattc tggtaaaaaa gttcagaaaa acacttgtta tcattcggtt
    11581 tggtgaaatt aatgttcaaa gtaaggataa aaatcaaaaa gatagtagcg gaaccaagtg
    11641 tttatcagta agatagaatt tggaggtcag atcatgctta ttgaagaaaa actaacaaaa
    11701 caagagttgt ttaccacgac agagaaacgg attgtcgatt acatccgtag gaatatcgaa
    11761 gctgcagtct acatgacgat tgaagaatta gcaaaagcta cgtatacttc ccattcagct
    11821 atcattcgtt tatgcaaaaa aatgggattc agcggattta aagaattccg tttcgagctg
    11881 gggcgggaag ttcatcaatt gattgctcag ttcaatcaaa cgattgatcc taattttcct
    11941 ttttcaagtg aagatagcac tcagcaaatt gcaaagaaaa tggcggattt atcgatccag
    12001 tcaataaaaa aagcacaatt gcagattgaa catgaagatt tagatcaaat cggtaaagta
    12061 ttgaacaagg cacagcgaat ctttcttttt gcaaaaggtg attctcaaat cacggcaagg
    12121 aaattccaga ataaaatggt caagttgaat aagtttttga tcatggctga agaatacagt
    12181 gattcttctt ggaatgcagc caatttgact agcgaggatt gcgccatttt tattagctac
    12241 agcggtcgca tccatcatta tgaacgaata atgacctatt tgaagcatgt agaggcccct
    12301 accttgctga ttaccggaaa tcagcattca gaaatggcca aacaggctga tatgtgtctt
    12361 gttatatcgc aggaggaata tgattttgct aaagtagcga ctttctcctc tcaaattgcc
    12421 ttcgattacg tattaaatac gttattctca gtgatatatt ctcaaaattt tgaagagaat
    12481 atccgcaact tgaaaaacaa acaagaattg atgcaaaaag gccttcttaa agaatatcgg
    12541 aaaacttaag aaaagatcct catttagtaa taagaaagat agtgtttgat aggctctctt
    12601 tgatatcatc ataaaaacag gctgaagttt tctttttcaa acctcagcct gtttgtttac
    12661 ggaacgattc gaatagatat attatagaaa tgaggaatag ctaacttctg tttcttattt
    12721 tgaacgattg acgaaaaaga tatataagaa ggaggaaaat cagtatacat gcagctaaca
    12781 atccatacag gaaaaatcgt ttgttctcgg atactttctg tacttctttt tgaacttctt
    12841 ttgcatgata aggaacccgg tgccctctga caagtaagcg atgactgttg accatataag
    12901 gtgtacaagt taggagtgtc acgaaatcct gccctttttc gattctcaga tcttttgtat
    12961 cagtcggttc taccgtcttg atctgatcga cttgataagc gagtgtccgt ccattgattt
    13021 cgatatagaa agaatcacct aaagtcagtt tcggtaagtc tgtgaacaac tttgcttgag
    13081 gcagtcctcg atggctggaa ataacggcat gtgtattttc tccccccgta ggataagacg
    13141 ttccttccaa taatgaggca cctttttcta gcagcacagt attcgtttca tcaaaaattg
    13201 gcaaatggac attgatctca gggatcgtca aaataccgat cgtatgtttc tcaaaatagg
    13261 ttctatcctt tttcgcaggt tcttttttct tagtaaaggg atctgcacct ggatttccac
    13321 cttcttttgc taattgctgg tttttctttg tcatgttttc ttggatttta gccatgactt
    13381 cttcattttc tttgaccgct tgttgctgat aatgagagat gatctgctga tccagataat
    13441 tattcagcgc gtcgctaaca aatggataag ctagtatccc tattcctgag agaagcaaga
    13501 aaagcatcag cagatctaga agtttccttt tctttggttt catttgtgtt ctccttgtga
    13561 tgactgtttg ataataaaag aaaaaggagc atttgcgtaa tccgctgctc ctttttccaa
    13621 ttcttgcaaa aagactaaat ctgactgtgc ttgcgtctag caaagtacag tccagcaatc
    13681 agtagaagga ctactcctgc accgatatac acatagattc ccttaccgcc tgttgaagga
    13741 agtgtacctt tgtgtttatt tggtattttt tcaggagaaa tcaactgtcc tgctgttaca
    13801 taagattgtt catcgatagt aaatgctaca cggtttgtta atggaacata tttttctggc
    13861 gctttcgttt cttccagata gtacgtgcca tattttagac ctgtcacatc gattaaaccg
    13921 ttacttgtgg ttgtaaaaac cgttgctgat tctttcgccg atacccagct gacggctttt
    13981 gtggatgggt cgatcgataa atatttcgct gtgtcactat cttgatcacg aacgacgaat
    14041 tctgctccag caagtgtttg gtctgatgta acgtcaccat ctactttaac aaatcgtttg
    14101 cccccagtaa cgacatcgac tgacggtggt gtttgatcat ttgtatgccc gttatcgaca
    14161 tttgcttggt tagaaaatcc tttggttgga tctgcttttt cgttcaaatg catatagtaa
    14221 acgaatttca atgtaccgcc aggagttaat gaaggaatat aattcggatc aactgaaacc
    14281 gtgaatccgt ctgtttgctc agtgacagaa taatttactg ggtcgatttc tttatttcca
    14341 tcatataagg cataagcata cgttcctgaa gaatcattat caaatgttaa agcagcgtca
    14401 tgagtatcga taagtttgaa tgttgtgtac ttgttttgcg ttccttcttt atcagcgata
    14461 cctaatggga tattgacaga aatctcatat tgaatttttt caccgattgc gacatctttt
    14521 ccattcaatt gaggtgttgt tttatcaact ttagacgtat catttttgat ggtcttttct
    14581 actggtgttt ggctatttgc caggatctca aaaggcgtga ttgtttgctt ttcaatcatt
    14641 tccgcattat caggagcttt tacttcttct aaattatatt ttccaacttc taaatgatta
    14701 acgatcaact ggcctttttc aatagatgcc tcggcaaagt cattgttgcc gatgtcataa
    14761 gaatgaccag taatgaaatg tttggctttg gtttgatcag ttgtccaagt gtacaatcca
    14821 tctgtgacac tttggatgta ttttttgacg cttggtgttc cttcttcttt agaaataata
    14881 aattctgctc catttagtgc ttcgttttcg gcagtaccga tttttgtaac tttcaacgtt
    14941 ccatcattac cgactgtatt tttagggtag agatggatag tatctagttc ttctgtcccg
    15001 tatttataag agccatctgc ttgtttgatc atctcatata cagggaaagc taaaaccatg
    15061 tttgcggcag ctgacactcc gtcttttggt tcttctttga tcgtgtagac tgcatctttc
    15121 ccattttgtt ttttaggtaa agataaagtg acatttccat cagcatctgt cgttcctgaa
    15181 gcaactggtg taccaggagt caaagactgg actgcttgtt ttgctgcatc cacggacgct
    15241 cctttatctc gttgcgcata aaattcttga gtgacgttat aaactgaaaa tgaaatatcg
    15301 gctaatcctt ggtattgatc gaattcgctc atttctttcc cgctgttttg gattaaagga
    15361 tcgggtaaat cagtcatttt ctttttatga aggatgactt gagctgctgt ctcctctgca
    15421 aacacttttt ttgcgccgaa gctgtttgtg agtaatggta aaataaggaa aaggactcct
    15481 aacataacgt ttattttttt atgatttttc attttttcac tcctgttctt tttttgataa
    15541 acaaccagat tgcaaagctt gcgaatacca taccaacaac gcctaaaagt gtatttttcg
    15601 tttcgtttgt ttttggaaga aatggacggc tattttcttt tttattgact actttcaata
    15661 tactgtccgc ctctttatcc tcagaaagaa aagttgaaat ggtaaacgga atttctgttt
    15721 cacttaaaat ataaccagaa ggtgcttcga tctcttttaa tcgataactt cctgttttca
    15781 acccggaaat tgaaaaagcg ccatttttat cggaaatcag gtgaagcgca tctgattcgt
    15841 tctttgtcca cttgtatccg ttggctgttt gctggaggta atttcctgct ttgtttttaa
    15901 taaggaatac tgcgccagct aatcgtttat ctggtttatt tgtctcgatt ttgacaaatt
    15961 gcttactccc tgtatataca gagactttct ttgtactgac accaaaacca aaatccagtg
    16021 ttccgttgtt gttgatttcc ttgttcgcct gtgctgtact gcttagctgc atctgataag
    16081 aaatggtcag tttttttcca gcatattttt ccaacgaagg gatcgaaaat aaaacatcaa
    16141 atccgtgagg tttcttgtgt atgacaaagc cttctgtcag cttttctcca ttcgatgaaa
    16201 tcgttaaact attaggtaaa aacgttaatg cttcatccgc actatctgac aattcgaact
    16261 tttgatagtc aaggatattt acaggaattg tcgtatgcaa ggaataagtg attttttcac
    16321 caatcgtaaa atcatttcta ggctcttcta ataccttctc aaaaggtgga tcagggtagt
    16381 cgttttcttc atttttagga tacaaatgga tagttgaaag tttttgattg ttttgtccat
    16441 gtacaggaag aacaactacc atattctctg ccttttcttt gacgacttct ggtactttgg
    16501 attcaatgaa aagatacgct ttatctcttt tttcttgatc tttagcggcc agagaaaaag
    16561 aagcgatacc atcttcgttg ttaagagttg tagttgttgc ttctgcggta aacataccgg
    16621 aagacgcacc gttttttgcg atctctgctt gtgcttcttc taccgttttg cccttttccc
    16681 gtagatggta aaaagaatct gtgacatcat aaacttggaa tgtgacacca tttaatcctc
    16741 gatacgtttg taataaagct ttttcttgtc cgtcatttgg atgatttttc ggcagttgcc
    16801 cgtttgggaa aagcaatttg tgtaaagtaa tctgaaccat ttctgtttct gtttctgttg
    16861 ctacctgagt aaaagctggt ttaaatagta acaagaagag acacatacta agccaaccaa
    16921 gttttttcat atttagctgc cccctctttc ttttttcaaa aaaagatacc aaatagaaaa
    16981 cgcacaacca atcatcccta ctagatagat tcctaaacgg ccaattcctc ccgtttcagg
    17041 taatggtact tttgcctgat tcgtgatgtc taaagaaatc tggttgtttt tggctcctgg
    17101 tgacagaaca gattcatgat cttgtccatc cacttgaatt gacccatctt cgtgtataac
    17161 tatagtaact ggctctttta gaccttggta tccttctggt gtaaaagttt cggtcagcgt
    17221 ataagttcca ggttttaaat tttcgaatag aaaggtatct gtttcttgtc catcttttgg
    17281 cgattctaag tccatttctg gtccctgcag tctgaatttt gctcctttta atgtctgtcc
    17341 gttgtcttct tttttgtgta ccgttagatc aaacgctttt agtcgatttt gatgagtgag
    17401 cgtccataca ttatttgtca tctcggtatt ttttcccgtg taaatgattt ttccatattt
    17461 atcgatttgg aattcataat ttcccggcga agtatcatat ccggcaggac cagctgtttc
    17521 aacgactcga tatttaccag gtgtgagtga atcaaaactg gcaagacctt tttcattcgt
    17581 tgtttggctg tcaattggct gataagtacc atttgcttct tttttctgta gggaaaatgc
    17641 tgctccttta agatttatct ctttgtccgt cccttgcatc gcatacttac gtactgctac
    17701 aggaacttca acaaaaggat tttcaatagt caactggatc gtatcatcgg aagtagtgac
    17761 tgtttttcca tcaatggtta ccgtaccatc agaagcgatc ttgatttccc aagtagtctt
    17821 tttgcttaat ccatgccctt ctggagcttt tgtttctgtc agcgtatagg tcatttcttt
    17881 ttgcaatttg acattttctg gaagagaata ggttccgtcg ccatgatctg ttagtaaagt
    17941 atcaatagaa tctcctgtta atttgaaaac agcgccaata agatcctttt cacctgtaga
    18001 ggaatttttc gttattttca agtttaacgg tgtgaattgt ttagtatttt tccatgtcat
    18061 tgcatctatt tgttgagaat cgtatcctgg tacaggtaat tctttgattg tttggtaact
    18121 gaatgcttga ccttgattat tgtattgagg tagtgataat atctcttgat aactttctcc
    18181 gctatttgca gataatttgt caatgtctgc acgttcccac gtatttgttg tatctttagc
    18241 tggtttaatg attcgaatat atccgttttt ccaagctgca gcatttgtcg tatgttcccg
    18301 ttgaatctca aaagtaactt gatctggacg atcagctaga ttgttgtcaa attcttccca
    18361 taactttttg atgtgaagac tgactccggg agctttagca gatggtatac cgaattcagc
    18421 taattcattg gtatcaatgt ttggctggaa agttgtcctg ccgttcattt gataccagaa
    18481 atttggatgg aagtcctcat tttctgtttg gattctcact tggtaatgga tttggatttc
    18541 ttggtctttt cctaaataga tctgattgct cttgatggta tttccttcta tggaaataga
    18601 tggagtgacc gttgtaggac ttgtccccac actcttgact gaaagtgtac caggctgata
    18661 aatgaatggt tctgcgattg gatcatttat ttgtccattg cttacagttg ctgagatctg
    18721 tacagctttt ttggctaggt attcagagat atctgttgca tgatcagctg attcatagta
    18781 aagatcgcct ttttcatctg atgaaaccat ttgtcgcata cgagactcta cttctgcttt
    18841 tgagagacca gctgccggat cgctttgaag ttggatgcca agaccatgta tttcgattcc
    18901 tcgttctttg agtgccatcg cttctccgat ggttgcgata aacgtactgt cgattcttct
    18961 ggatagattg ttttggtcag gtgcatcata gattcttgag attagagaag tatttcccgg
    19021 ccgatcttgc gtattagaaa actgagttcc gtaataattg ctgcttgatt gtgcgtgtac
    19081 ccgctgtact ttataggaaa atgttggtac accatccgtc agcaaaacga tcactttttt
    19141 atgtccattt ggaacggata gcatgcttcc tgcatctctt agtgcttttt gtgtaaaagt
    19201 accaccattt gtccgtgaag gtgtaatgga ttttacttga tttttcactg aatcaaatga
    19261 acccatctgt actgcaccgt tactgtagct atatccttcg cttgagtagc cgacatatcc
    19321 catattgatt ttgtctgtga taccgctatc tgctaaagta tcgacaaaac gatcgacacc
    19381 aatctttact tcaccgatcc gattattgtc gttcatactt cctgaccagt ctacgaccaa
    19441 tacgagatca agaggcgtga tatctttttg tacatttcca cgagcattca aataaacatc
    19501 aaacaatccg ggtgtacttg tttcctttgc atactttcgt atcgcatagt ctgcttgatt
    19561 accggttcct ccatattcga tatacgaatg ggtccgatca ttaggatctc cattccaaga
    19621 atttatacca tcccattgat ttgttccgtt tttattgcct tgatgatcaa gaacatttgt
    19681 atttcctgta ggttgccaac tggctttagg ataagtgccc tgatcatctg ttgtatattc
    19741 tgtaggaatc gtttgattag atggcacact cacagctggc aaggataatg taggcaatgt
    19801 aaatgggctg acagaatttt cgttttcttt tgatgattct tcttctattt cgtctggctt
    19861 tggtactgac acatctgtat ctttcttatt ttctgtctca tcaataatgt catattcttt
    19921 attatcgacg atcagcttgt ttgcctggct gtggtaaagt gctggatcga ttggaaaact
    19981 cagatcgata gtttgtttag tgtttgatga gatttccaac gtaattttcc cgctatttac
    20041 ctgataagca tagccatctc cttcttttct ttccacatca cttaagtaat tttcgactag
    20101 accaatttct ttttctatct tttcagcact ggtgttattc gcatttagct tgatgttcag
    20161 tgcttttttt ccagactgat ccgaaatgac ttcagctgaa attgtttcta aaactgttgg
    20221 atcatctgta gtgtctgcta ttgcagataa tggcacaaag tttgataaac tcaatattat
    20281 cagcatgaaa ataactttca gtttcttccc tgtggtcgtc atttttcccc tcctttttct
    20341 ttttttctgt tttgacgtga atagtgaaaa ttattaaaat aaacaggcgt tacataattt
    20401 tatttatttt aatatgataa ttattatacg aagggcgcta aagggaatca caccaatttt
    20461 cataccaatt ggtttcattt atgaagttga ttccttcgat taatgagcgt ttcattgaaa
    20521 gaaaagggtt gagggagaaa aacatatgat caggttgttg gaattaaact atagaaagat
    20581 ggaaaaaatc attcgaattc tgtccatttc tgacaagtgg atgactaaaa aagaacttgc
    20641 caaacgaatt ggtagttcag aatctacatt tgtaaggtat atcgaagaga tcaaaatccg
    20701 ctggggaacg gatgtaacga ttcaatcttc acaaaaattg gggtatcgtc ttgaacattt
    20761 gaatgtacca gcttatttaa atgttctgat ggaaatgtcg caaagttcta caaacagtcg
    20821 tttattaaat gagattattg tcaatccggg taaaacgatt gagtattatt gtgaaaaaat
    20881 ttccatcagc cggtcttctt ttacacgtaa gttgaaacaa tgcaatcaaa ttttagaaag
    20941 ctatttatta aaagtgatcg ttgaccaagg ttttcagtta actagcctta aaagtgagct
    21001 ccagttgcgt atctttgtta cctttttctt tttaagttac tacggaagac gcacgttgcc
    21061 atttccaatc gaaaaaaaga agatcaaaga aattatggtc aagaataatt gtcagatgaa
    21121 tttgtttcag gaagaaagcc catatgagca atctttcttt attatgtatt ttgttgtgca
    21181 tctcatgaga gaatcccaag gaactccatt tgaatggaaa gctggagact cttatgtaca
    21241 gttgataaat atcgataaaa aagacttctt aatcttgaaa aatctcttag gaaaattgaa
    21301 gtacagcagc tatataaaag tagtgaacta ttttgtcaat acgatttttt cagaattttc
    21361 ggagaaagaa aaaaatgaaa tcagtgagca gatacgaaaa aatatgtcct actgttcgtt
    21421 ttcatccgct gattgcaaag aaaaaaacga agaatatgtt gttcaattat taacgcaaat
    21481 ttattttttc tcccgtattt tcccatacga tacgactcat ttgacgatcc gtgcaactta
    21541 ctttgcacag cagatgcaac accgccagat acgtctatat gctggatttc gtgcagaact
    21601 tcaattttat tccactctac tgaatcagaa tctgatttat tatttatcta gctatatctt
    21661 ttggatgctt atcggtaatc ctgaaattca ccattatact tctgataaaa aaatactgat
    21721 tatcagcgac ttaagtcttc gacatagtca atatattgaa gaatatatca gtgatattct
    21781 tgctgtgcat aaaattcact ctgagacaac tgctattact gaagatcagc tgtcgaaata
    21841 caatcttctc gagtatgatc tgatcgttac gaatcaaccc attttgaatg cccatgttcc
    21901 tcatatattg attgatgata gtgtgtcatt cgcaaatgaa gaagaactca tccgcttatt
    21961 cgagttatga ataatcaaaa aacagctatg tataaaccac cctgataaaa atttcaggac
    22021 aaatacacat agctgtttct ttttattgta ttccttcagc aatccaacga gatttatagc
    22081 gaaagtcttt taacaactct tcttttgtcc aattatttgc aatgagatcc gtcaaagtgt
    22141 tcattacgag ggtcatggaa aagtatagga aactaactgc ttcttccgac gattgaatgg
    22201 agaaatgatt gattcttaat aattccagcc atttttcgtt cattggagaa tcagccgata
    22261 gttctctacg tttggttgtc tgctgatcat ttcgctgggt taacaagtat aacctttgcc
    22321 aatagatact ttcgtgatct aagtcgcagc accactctaa atatttttcg atccccaaaa
    22381 agaaatgttg tttgttttcg ctaatgaatt ttaagatatc ttgatggact atgtgagaac
    22441 tgcttttgat cgtatacaaa taagcatctt ccaaatcttg aaaatattta taaaaagcac
    22501 cgcgcgacat ccccatcttg tcaacgattt ctgagacttt gacttggctg atgtgctgtg
    22561 agcaaaatat ctccagtaat aggtcatcga ttctctgttt ctttgcgttc gataaattga
    22621 aaaaagtttc ttttggcatc cacatctctc ctttatctct gattatacgt ggatagccct
    22681 tcattgtcat gaaggattca ttccttcttt ttctcttctt ctgtcaagta cccatcttcc
    22741 attcgataaa cgcgatcact ccattgaatc atacggacat catgagtaac cataatagtc
    22801 gctttttgtt tctcatgagc ttctttaact agtaatttga cgacttcata tgcatgatct
    22861 gtatccaaac tagctgttgg ttcgtctgct aagatcaagc ttggctcatt atataaagcg
    22921 cgagcaattg ccactctctg tctttctcca ccagaaaggt ctttaggata actattttta
    22981 agtgactgga tatctaaaga ttgcagcaag gaatcgattt ttgttgaatc ttcttttttt
    23041 gaagtaactt ggtcaactag ttcaaattgt tcctttaccg ttaaaaaagg aatcaaatta
    23101 gagctttgga gaataaatcc tatttcttta aagcgtaaat ctgctcgttt tttttctgga
    23161 agctcagtta gtgagtgacc gttgatcact atttctccgg aagtcggtgt ttgcaatcct
    23221 cctgaaatgg taaggaaagt acttttacct gaaccagaag gaccaatgat gctgacgaat
    23281 tctccttcct ttacataaaa atcaattgct ttcaaagctg agacctttgt atgaccagaa
    23341 ccaaatgttt tggtaacttt tttcatcgtt aatatttttt tcatttttat taacctccta
    23401 ttgctgtaat tgggtctact ttggttatcg tacgtatcga aaacaatccg cctagcattg
    23461 cgacaatgat caaaatccca ctatatagca accattgtga ccaaaaaaca gcaaaaggca
    23521 tcgcgcttgg taaaatcaga ctagtcaaat aagcaaacaa aagagcaagc agaacgccta
    23581 ccaccccaac aataaaggac tgtgctacta aagatttagc gatgaagaaa tttttgatcc
    23641 cttgtgcttt cattactcca aaaatagcag tcttctgaag tgtcatcacg tacatgaaaa
    23701 taccgaccac ggcagcaacc actaagaaca aaaagtatat catcgcattt aaggtcatat
    23761 tttgggcaga atagccagga atactttcaa tgaatgtccc aatagttaat aactgtagac
    23821 ccttcgatgc tttttctttc gaaattttag ctgaatcctt tattacgatc ccattgatcg
    23881 gctgttcttc ttcagaagca aacggctgtt gtccatactt caaagcagtc cacgtatcta
    23941 gggacgcata gatgacggga gaaactgtgt aataagtttc aggaaagata ccgacaatcg
    24001 tcaaaggatc gtcgtaactg ccaattttga tcgtatctcc aattttatag cctgaatcag
    24061 ccagattttg agagatgatg atttcattct ttgttttaaa ttcatgccct ttcgtgagtt
    24121 ttggtaataa aaatgcttct ttcgttgtgc cgaaaactgt aacattttct ttgttttttc
    24181 ctttgattgc tccgctgtac aatccgattg gtgctttctc tcctccttca atgtatttca
    24241 gatctcctct tgttaattgt gaagctgcaa atacttgatt tgcatcctcc gacaaaacaa
    24301 tctcttgtgc gtcccaatca tcgattgctt ttttaaactc ttctgaaagt ccattcgcta
    24361 gtcctgacaa catgaatacg acatacgcaa tcaagaacat aataccgacg atcaagccat
    24421 atcgtaattt agaatagcga atttctttga tagctagaaa catttgtttt tcctccctat
    24481 gatagattga tagtgacaaa atgtcacttg ttgaatagtg taactctaaa gtgacaattt
    24541 gtcacttgtg ttgttttttt cttatcaata aattgtctaa gacatctttc gataaaacct
    24601 taacatgagt cataaaaaat ttatttaagc cttatttatg ctttccatga taaactaaag
    24661 tgaccaaatc aatgaggtgg gaaaatgatt aaactattag tagtagaaga cgataaagta
    24721 ttatccaata atataaaaga aatactcgct gcaatcggcg agataacaca agtttatgat
    24781 ggtagtgaag gactgttcga gttggaaagt ggagtttatg atcttgcaat cttggatttg
    24841 atgatgccgg aaatgaatgg atatgaagtg ttagcaaaaa tacggaaaga aaaaatacag
    24901 actccagtat tgattttgac tgcaaaagat agtttggaag ataagattga aggatttgaa
    24961 cgaggtgcag atgattatct aacaaaacct ttctatcgag aagaactgat catgcgcgtg
    25021 aaagcattat tgaaacgctc gctaggtctc tttgatgaaa atgttctgaa gtataaggat
    25081 atcagttgtg atttaaaaag caatgaagtc ttgtataaag aacaagtttt gcccatacaa
    25141 ggaaaagagt ttgaattgct cgtttatttt ttacaaaata agggaatcat tttgacaaaa
    25201 gagcagatat ttgaccggat ctggggattt gattctgata cgacgattac agttattgaa
    25261 gtttatatga gcaacttacg aaaacatctg cgaagtactg gattggataa agaaatcaaa
    25321 accttacgta atgtcggtta tatactgcaa agggtgtgat gcaatgaatg gagatattac
    25381 acgaaagcaa cgtagtcgct tttttgcgac aaatgtagca gcatttgctt ttatcttttt
    25441 tcttttggga ttcattactt tacagatatt aaaccaatct gcatacagag aaacagatct
    25501 gtcattagag aaaataaatt ttgattctcg aacagtacaa ttagagatac aaagatatca
    25561 gcaaggcgat ccctttttaa acccgactca aacggaacaa aacagaacaa acatggaaca
    25621 gccattaatg gaaccatcaa atgaccgttt taacactcaa gtgatcttgt ggtctaaaaa
    25681 tggtgagata ttgaataaag cagctattgg tgggcgtttc aaccaaatca aagaattaaa
    25741 aatggatacg aaaaatctga ataccatcca aaatattgaa ttaaatgaag acagtggctc
    25801 atctgccctc tctttccgtt cgattacgaa aaaagcggag acgaatcaag atggcgttgc
    25861 ctacatccaa attttagaaa atacaaatca aatcaagaat tcattagcga cttttcgcac
    25921 gattatgatt ttgtgtatga ttgttttttg gattctctct attggtatca gttattactt
    25981 gtctagtcta agcatgcgcc ctattattct ttcttggaga agacaaaaag aatttgttga
    26041 gaatgcttct catgaattgc gcacaccgtt aacgattatt caaaatagtt tggaacatct
    26101 ttttaccaag cctaatcata ctattattga agaatccgaa agcatcgctc aagcattgtc
    26161 agagacgagg cgcctcactg gattgacttc ggatttgctg acaatcgctc ggaatgattc
    26221 tgatcagcag ctgctttcaa agcaaatgat caatacacaa gaatatatcg aacaacttgt
    26281 taaaccattt caagagatgg ccatcataga tggaaaagag tttattctcc ataacgatgc
    26341 aacattagaa gtaccaattg atgaaaaaaa aatccatcaa gttttggtta ttttgctgga
    26401 taatgcatta aaatatacga atccagaaga taagatcatt ttggattcat ctgttaacca
    26461 taaagaatgg ctgattcacg taaagaatac tggcccttct atcagtgaag aagatcaagc
    26521 aaggattttt gatcgatttt atcgggaaga tcaatctcgg gcgaaagaga ctggcggata
    26581 cgggcttggc ttggcaattg caaaacaggt aatcgaacag catcatggac agatttctgt
    26641 atccaattat cttccacaag gagtcgattt taaagtccaa ttgccgttga agcctaaaaa
    26701 ataatgctta tccctaaaaa agggctgtga caaaaatctt gtcacagccc ttttttgttg
    26761 cgaataaacg gtgttccaga agtagcttct tcggaaataa gccgaaattt ctttaagctg
    26821 accacttctg tcacaacctc tttagcttac gcttacgatt ttttattgcg gtcttcaatg
    26881 tgtacatcac cgcttgtgtc aatatccaat tcttctcggc taacatcttc tgtcactttt
    26941 tcaacatctt catgttcttc tttatgaatc ccaacttctt cacgaataac tgtatgtttg
    27001 cttacatcta cttgctcttc tttgattggg atagtaatcg tgtcatcttc caatttttcg
    27061 tctgttccct gtgaagattg atctgttact ggtttgcgtt cgatgacgat ttcttcacgt
    27121 ttgaccggta cttctaccgt ttcagtatcg ttgaccacat gtttgtggat gtcgacttct
    27181 ccagttgtca catcatgtgt atttacatct aatttttctt cttttagttg aattgtatca
    27241 tcatctagat ttggtttgcg atcgatatct tcagtcttgc ttgtacccat tgtatcagtc
    27301 tcgccaggta cgtccatcgg atcagttcct ggtgtcatat tcaatggatc cgttgtattt
    27361 tgaaagcctg tcgtgccacc tacgtccatt ggatctgttc cagtgccagt ttctaatcca
    27421 gtccccattg tgcttgctgg gctaacagta tcttgtgtat ccattgtatc ttctgttgtt
    27481 tctggctgac gatagccttt tactgtgatg acaaattttc catcagaaat atctcctcta
    27541 tattgatata aaggatcatt ttctggtgta gccgtctcag aatcataggt atcaaaagaa
    27601 aaagtatctt ttattttttc ccataaagaa cggtcttctt caccatccat ccgattttca
    27661 tctgtttcaa catctatacc agacaatccg tcaaatccta gtctagatgc tgcttcatca
    27721 gtagtataaa taacgatatc atctcgttca tacccttcgt tacgcaaccg ttcaattact
    27781 gataatgctt cttccctcgt actatagctt ccgatgaccg ttaaattttt ctcttccatg
    27841 taaattcctc ctagcaaata gatttatgat tg
//
LOCUS       DAXWOO010000018        27611 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.018, whole genome shotgun sequence.
ACCESSION   DAXWOO010000018 DAXWOO010000000
VERSION     DAXWOO010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.018"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            180..1946
                     /locus_tag="ACGE70_000624"
     CDS             180..1946
                     /locus_tag="ACGE70_000624"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFF8692280.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2048..3307
                     /locus_tag="ACGE70_000625"
     CDS             2048..3307
                     /locus_tag="ACGE70_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFF8692281.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3454..4755
                     /locus_tag="ACGE70_000626"
     CDS             3454..4755
                     /locus_tag="ACGE70_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8692282.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4758..5609
                     /locus_tag="ACGE70_000627"
     CDS             4758..5609
                     /locus_tag="ACGE70_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFF8692283.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5622..6437
                     /locus_tag="ACGE70_000628"
     CDS             5622..6437
                     /locus_tag="ACGE70_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFF8692284.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6588..7754
                     /locus_tag="ACGE70_000629"
     CDS             6588..7754
                     /locus_tag="ACGE70_000629"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFF8692285.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            7984..8631
                     /locus_tag="ACGE70_000630"
     CDS             7984..8631
                     /locus_tag="ACGE70_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFF8692286.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            8715..9659
                     /locus_tag="ACGE70_000631"
     CDS             8715..9659
                     /locus_tag="ACGE70_000631"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFF8692287.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            9794..11245
                     /locus_tag="ACGE70_000632"
     CDS             9794..11245
                     /locus_tag="ACGE70_000632"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFF8692288.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            11266..12102
                     /locus_tag="ACGE70_000633"
     CDS             11266..12102
                     /locus_tag="ACGE70_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFF8692289.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            12102..12761
                     /locus_tag="ACGE70_000634"
     CDS             12102..12761
                     /locus_tag="ACGE70_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFF8692290.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            12887..13576
                     /locus_tag="ACGE70_000635"
     CDS             12887..13576
                     /locus_tag="ACGE70_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692291.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            13582..14538
                     /locus_tag="ACGE70_000636"
     CDS             13582..14538
                     /locus_tag="ACGE70_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFF8692292.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            14582..15094
                     /locus_tag="ACGE70_000637"
     CDS             14582..15094
                     /locus_tag="ACGE70_000637"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8692293.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(15350..15898)
                     /locus_tag="ACGE70_000638"
     CDS             complement(15350..15898)
                     /locus_tag="ACGE70_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFF8692294.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(15987..17168)
                     /locus_tag="ACGE70_000639"
     CDS             complement(15987..17168)
                     /locus_tag="ACGE70_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFF8692295.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(17404..18912)
                     /locus_tag="ACGE70_000640"
     CDS             complement(17404..18912)
                     /locus_tag="ACGE70_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFF8692296.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            19071..19262
                     /locus_tag="ACGE70_000641"
     CDS             19071..19262
                     /locus_tag="ACGE70_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692297.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            19465..20382
                     /locus_tag="ACGE70_000642"
     CDS             19465..20382
                     /locus_tag="ACGE70_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFF8692298.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            20375..21448
                     /locus_tag="ACGE70_000643"
     CDS             20375..21448
                     /locus_tag="ACGE70_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692299.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            21596..22114
                     /locus_tag="ACGE70_000644"
     CDS             21596..22114
                     /locus_tag="ACGE70_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692300.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            22095..22487
                     /locus_tag="ACGE70_000645"
     CDS             22095..22487
                     /locus_tag="ACGE70_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFF8692301.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGESATTGAKEIKLNDGQSVIFNLSKL"
     gene            22563..23498
                     /locus_tag="ACGE70_000646"
     CDS             22563..23498
                     /locus_tag="ACGE70_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFF8692302.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            23631..24410
                     /locus_tag="ACGE70_000647"
     CDS             23631..24410
                     /locus_tag="ACGE70_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFF8692303.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            24403..24720
                     /locus_tag="ACGE70_000648"
     CDS             24403..24720
                     /locus_tag="ACGE70_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFF8692304.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            24964..26574
                     /locus_tag="ACGE70_000649"
     CDS             24964..26574
                     /locus_tag="ACGE70_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFF8692305.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            26731..27579
                     /locus_tag="ACGE70_000650"
     CDS             26731..27579
                     /locus_tag="ACGE70_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFF8692306.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT         8935 a         4883 c         5545 g         8248 t
ORIGIN      
        1 aaatagtaca ggaagtagat taaaactttt aaaagtttta agtctatcta tttggcaagt
       61 ttaaaaaata aaaattcggt atcaaaaaac tgttaccgat aacatttttg aaagcgttgt
      121 cttttgtttc tctatcactt atagtgatga cgtcatatta aaacacaagg aggaaatcaa
      181 tggatacagc tgcaatttat catcgaccgg aaagtgaata tgcatttcta tatacgaata
      241 caagatttcg tattcggctt cgtactagga aaaatgatat caaaaaagta tatgtgcttt
      301 gtggagatcc atataccata acaactgaaa aatggtatca aaaacaacga ccaatgaaaa
      361 aatgtctaag taccaatgtt catgattatt gggaaattga agtgacgagc gaaacaaggc
      421 gcttacagta tgcgttccat gttgttgggg aagataacac ggactgtttt tacggtgatc
      481 aaggaatttt tccttatcga gaaaatgtga ttactgaacc aaatttttat tttcgaatac
      541 cttattttca tcaaatcgat cgattcacta ttccagaatg ggtaaaagaa acagtatggt
      601 atcaaatttt tcccgaaaga tttgcaaacg gggacaaagc aaatgacccc gaacacactt
      661 taccttgggg aagtaaagat ccagaccgag aagacttctt cgggggagat ttgcaaggtg
      721 tgagggatca tttggattac ttgacggacc ttggcgtcaa tggcatctat ttttgtccca
      781 tatttaaagc cacatccaat cataaatacg atacgattga ttattatgag gtggatcccg
      841 cttttggcga taaaaaactt ttaaaaaact tgattgatga agcacataaa agagggatac
      901 gtatcatgtt cgatgctgta ttcaatcata tgggtgtcca ttcaccacag tggcaagacg
      961 ttctcaaaaa cggagaaaaa tcgatctata aagactggtt ccacatccgt tttttcccag
     1021 tagattctta tcagatgact gacttaccag aaactgctga aaatatccca tatgatactt
     1081 ttgcttttac tccattcatg ccgaaactaa atacagcaaa tccagaagtg caaaaatatt
     1141 tgctggatat cgcaacttat tggataaaag aattcgatat tgatggatgg cgtcttgatg
     1201 ttgctaatga agtcgatcat catttttgga aaaaattcag agaagcagtt acagaaatca
     1261 aaccagacat ctatatcctt ggagagattt ggcattcctc tcaagcttgg ctgcaagggg
     1321 atgaattcca tgcagtgatg aattatgcat tcacagattc gattaaagat tattttgcaa
     1381 aaaagaaaat cacagcttca caaatggtca gtggaatgaa tcatcagcaa atgctttaca
     1441 gagatcaggt gaacgaagga acattcaact tattagattc tcatgatact gccaggatct
     1501 tgaccctttg ccaaggtaac aaagaattga tgaagtctgt gatggccttt atgtttcttc
     1561 aaaaagggtc tccttgtatc tattacggga cagaaatagg gatgactggt gaagatgacc
     1621 cggattgccg caaatgtatg atatgggaga aggaggaaca agatctagag ctattcggtt
     1681 ttataaaaga acttgtttct ctaaggaaac agttgtctaa gattatttct gaaggttcta
     1741 ctcagtggct aatagtatat gatcgtgaag ataagctcta tttcacacgg gaactagagg
     1801 gacagatcat ctatgtttac tttaatcagt caaaagaacc ttgggtagta gaacaggaaa
     1861 atgaggtgat tcttagtcaa aattgtcagt tgttagaaga cgggaaagca gagatacgac
     1921 aatacggatt tttgatcatg tgttgaaaac tgttaccggt aacaacaaga aagcgtttgc
     1981 aaatgaagat ttgtgcccat tataataagg gtgtaacagc acgttgaata cacaggagga
     2041 aatgaagatg aaaaaaagaa gtgtgaaaca gttgggttta gggttaatct ctttaggaat
     2101 cttaggcgga ttagcggctt gtggcaatgg taactcttca gcagaagggg aagccgatga
     2161 aggaaaaacg ttaacagttt ccgttgattc tggatataaa gattatataa acgaaataaa
     2221 aaacactttc gaaaaagaaa acgatgtgaa aatcaagcta gtagaaaagg acatgttcga
     2281 tcagttggaa tcattagcgt tagatgggcc tgctggaaag ggacctgacg ttatgatggc
     2341 agcatatgac cggatcggtg cgttaggaca gcaaggacat ctggcggaag taaaactggg
     2401 aaatgaagca gcttacgatg aaacagacaa agcccaagtg acttatgatg gaaaaatcta
     2461 cggagaacct gcagtaatcg agacccttgt tttgtactat aacaaagatt tagtcgatac
     2521 agcaccatca acgtttaaag atttggagaa tctatccaaa gacagtcgtt ttgcttttga
     2581 gtcagaagca ggaaaaaata caggattttt agctaaatgg actgactttt attattccta
     2641 tggtttaatt gcaggttacg gtggctatgt atttggtgat gatggcacaa atccttcaga
     2701 tatcggtttg aataatgcag gtgctgtaga aggaatctct tatgcaacag attggttcaa
     2761 aaatgtttgg ccaaagggaa tgcaagacat aaaaagtgca ggagacttcg ccagccagca
     2821 attcatgtcc aataaaactg cagcaatcat tgacggtcca tggcaagcgc aaacatacaa
     2881 agaaaacaat atcaattatg gtgtagccaa aattcctact ctaaataatg gacagcctta
     2941 tcaaccgttt ggcggaggaa aaggatgggt agtcagcaat tatgctaaaa acaaagacct
     3001 ctctcaaaaa tggcttgatt atgtaacgaa tcaggaaaat caagaaaaat tctttgaaat
     3061 ggtaaatgaa atccctgcaa accaacaagc tagggaaaca gcaaaggcta aaaacgatga
     3121 attaacaaca gctgtgattg aacaatatga aacagctcaa gcgatgccaa acatcccaga
     3181 aatgggcgaa gtttggacag gtgcagaaaa cttgatgttc gatgcagcat caggttcaaa
     3241 aacaccaaaa gaatccgcag atgaagcagt aaaaacaatc agtgaagcca tagaacaaaa
     3301 atactgatta aaatgataac cgtccatttt tataaaaaat ggaacaggtg cttcagataa
     3361 aagtggcaag agcgagatgg aaaattatct tgctcttgtt gccattatct taaaagcagt
     3421 ttgcggttta gcaagtttag agggggcatt cagatgcagc aagataaaac aagcaatatc
     3481 aacgaaaaaa aaatcacgct ttgttcactt attccaggga tggggcagtt tgtaaatggc
     3541 caagctttta aaggtatcgt ttttttcgcc atattcgtcc tattcattgg tcagatgctt
     3601 ctgggcggat ttcatgcatt ggtaggattg gttactttgg gaagtatacc aatggaagat
     3661 cattcattat ttattttgat tgaaggcact ttgcaattca tcattacaat cattttcttg
     3721 attttttacg gtttgaatct tttagacgct tatcgagtag cgaagatgcg tcaagagggc
     3781 aaaaaaatca atcggacgat gaaggaagtc atacataata taggtgatga aggttttcct
     3841 tatctattga cacttcccgc ttatcttctc atgattttta cgatcatttt cccagtccta
     3901 gttacattgt tcactgcatt tactaattat gacttcaatc atattccacc agccagttta
     3961 attgattggg taggggctaa aaactttttt agtatcttct ttttaagctc atatcgtaat
     4021 actttttttg cagtcttttc gtggaccgtg atttggactt tatgtgcaac aactttgcaa
     4081 atcacccttg gtattctcac agcggttgtt gctcaccaga aatttattaa attcaaacgt
     4141 attttcggta tcattttctt attaccatgg gcggtcccag cttttattac tatcatgagt
     4201 ttttcgaata tattcaacga tagcgtaggt gccatcaata ctcaagtgat tcctttcata
     4261 aaccagcttc cttttgtaga tatcagtgca atctcttgga aaacggaccc gaactggacg
     4321 aaagtagcaa ttatcttagt gcagggctgg ttaggatttc cctatatcta tgtgatggtt
     4381 acaggaatct tacaatcgat ttcagaagac ttatatgaag cagcaaaaat cgacggagca
     4441 aatgcatggc agcgcttttc aaacatcacg cttcctgctg tcttcctagt ggcagcgcca
     4501 acatttatta cacagtatac ggggaatttc aataatttct ccatgatcta tctgtttaac
     4561 aatggtggtc ccggaagcgt aggcggagga gctggttcta ctgatattct tatttcctgg
     4621 atctataaac ttacgactgg aacttctccg cagtattcta tggcttcagc aatcacgttg
     4681 attatctccg tcatcgttat ctgtgtgtca ttgctggtat ttaaaaagac aaatgcgttc
     4741 aatatggggg attaacaatg aaaaaatttc attcgagtgc tcgattccaa aaacgacttt
     4801 ctcacttttt tacttacttt tttcttttaa tactgtcagt cattatcatc tatccgcttt
     4861 tgatcacagc aagtactgcc ttcaagtcag gaaatattgc agcttttagt ttagatcttt
     4921 caagtgactg gactttagga aactttactc ggctattcag tgaaacactt tacgggactt
     4981 ggtataaaaa tacattgatc atttctgtcc taacaatgat cgtacaagtg actgtagtaa
     5041 ctttggcggg ctatacttat agtcgctatc gttttatcgg ccgcaaatca agtttgatgt
     5101 tctttttagt gattcagatg gtaccgacta tggcagcatt gactgccttt tatgtcatgg
     5161 ctttcttatt agaagcgctc gataagtact ggttcttgac aatgatctat ataggcggtg
     5221 gaattccaat gaatacttgg ctgatgaaag gatactttga cactgtacca atagatttag
     5281 atgaatctgc taaacttgat ggggcagggc atttcaggat ttttttccag attgtattac
     5341 cgcttgtcaa accgatgctc gccgttcaag cattatgggc ttttatggga ccttttgggg
     5401 atttcatgtt agctaagttc ctgcttcgaa cacctgaaaa tcagactgtt gctgtcggct
     5461 tacaaacatt tatttctgat gtgacaaacc aaagagtctc tctttttgct gctggggcaa
     5521 ttttgattgc actaccgatt tgtctattat tcttcttttt acaaaagaac tttgttaccg
     5581 gcctgacatc tggcggaaca aaaggatagg agcagagtat catgcaaaca tttccgttaa
     5641 actatttttc cgcattacgt acgccaactc aagtgtttgc gggacgcaaa ctattatcat
     5701 ggcctaaatt tttcttgata tttgtatttt tagtttcctt gatggtcatg cctgtgactc
     5761 ttttttatgc caatcagatc caagcgattc cattagaaca atttctatcc gttcattcgc
     5821 tgatcgatga acaaggaacc caaaaatttt ctgaacttga attatctgaa actgggttac
     5881 aagcatcgca acaaacgata gctgtgaccc ctgaaatttt ggtaggtgtg tctttatctg
     5941 agaaacaaca atccgatcat ggaactttta ttgattttga gaaggaacaa tgggtgatcc
     6001 aacaaaagga taaatctggt atacgccgct atacgatgaa ttatagccca tctttccaac
     6061 cagattctgt acgtacgccc gaagatttcc aacgattttt agaaagagaa ttttatgcca
     6121 gtaatcgtcc aacaatcatt ttatcttata gtttaagtct tggtcttgtc ctatttgtca
     6181 tgacgagtct cattttattc ggggcttcct ttttcttgtg gatgacaaga aaaagccaac
     6241 tttcatccat tcatacattc aaagagagtg caaatttgat gctgaatgtt atgggaatag
     6301 gttccatggc agcagcagtt gtagggttta tccactttga ctttatcttg atgctaagtg
     6361 tccaaacaac gataactgtc ttgctgcttt tatgggtttt tgtcaaaaca agattcaaag
     6421 ataagcgtgt cgaatgacaa gtttaagcga tttatgcgac aatgaatgtg aaaaaaatga
     6481 aaaataaagt agtttttgaa aaatttctga aaaaattaaa cttcttagtt gattcttttc
     6541 ttttttagta ttaacgtaat agggtagttt ttttaaggag gaaaatcatg ttagagaaaa
     6601 ctttatacaa tcaattattt tcacgttctt tctcgctccc tgtcgaagta aattattggg
     6661 acggtacaac gaaacaatac ggagatacgg acactcctcc ccaaatcaaa atcaatatcc
     6721 atgaagaaat cccaatgaaa gagattacaa gtaacgcttc tttggcacta ggagaagcct
     6781 atatggatca tcgaatcgag atcgagggaa gtatccaagc attgatcaac gatgcttatc
     6841 agcaagcaaa cagcttttta cgaggaaaag attatctaaa atggctgcca aagaaagaaa
     6901 aacatacgaa acaaaaaaac aaagaagata tccatgcgca ttatgactta ggtaacgact
     6961 tttataaact ttggctagat ccaactttga cttattcttg tgcttatttc aatacacctg
     7021 aagatacgtt ggaacaagca caaatcaata aagtccatca tatcttagac aaactcttta
     7081 tcaaagaagg cgaaacgttg cttgatatcg gttgtggctg gggaacattg atgttcactg
     7141 ctaccaaaga gtataatgtc aaggcaactg ggatcacact aagtgaagaa caatacgatt
     7201 tgattaccaa aagaataaaa gaagaacatt tagaagataa atgccgtgta ttattaatgg
     7261 attaccgcga actaaaaggc gaaacgtttg atcatatcac aagtgtaggt atgttcgaac
     7321 atgtaggttc tgaaaatctt gaaggttatt tcaaagtggt aaaagattta ttgaaaccaa
     7381 aaggaacagc tttgatccat ggaatcagcc gacaacaagg cggtgcgaca aatgcttgga
     7441 tcaataaata tattttccct ggcggttatg tccctggtgt tactgaatta gttgataata
     7501 ttacaaaaaa cgatttgcaa ctgattgatt tggaaagctt acgtcgtgac taccaattga
     7561 cattagaaca ttggacaaag aattttcatc aagtccaaga tcaagtcacg aaagaaatgg
     7621 gtgattcctt ctatcgaatg tgggatctat atttacaagc ttgtgcagct tcatttgaat
     7681 caagtaatat cgatgtgatt caatatttgc ttgttcaacc tggaaacaat gacatcccaa
     7741 tgcatcgtgc ataattgagg caaaagtact gtttaaagac tatttaaaaa gaagctaagt
     7801 tttatgatgc tccagaagtg aacttttttg tctaacttct aggattgtct ctaaagctta
     7861 gtttcttttt ctttatattt tcataataaa agtttggttc tttaattttt aaacatgaca
     7921 gaaaattttt tcttcgttat acttgtaata gaatactatt ttataacaaa ggggatcgaa
     7981 tcaatgaaaa aaactgttat ttatactagt ttatttgcag ctttgttttt agctgcttgt
     8041 caatcaaata ccaatcaagg cacatctggg aatacgtctt ccagtagcga acagcgctct
     8101 tcacaggtat cggactcctc aacaaaagca agcacatctt cgtctagcca gacaaaaagt
     8161 tctagtgaag caagtactca gtcagatatc aaagtgtctg cagctcgtgc gatcgaattg
     8221 ttccaagata aatacccaga tactgcaatt actagtttgg aattagatag tgattgggga
     8281 tcttacttct acaagataga aggtgtcgat gatcaaaaag aatacgaagc aagaattgat
     8341 gctcaaaatg aaaaaatgga gacagagaat ccagaaactc tagatcgaga tgaacaaaat
     8401 ggtcaaaaga aaaaagaaga tggtctggat gttgataaat tgatcacgat tgaggaagca
     8461 gcaaaaatcg ccgtcgacaa agtaggaagc ggtacggcaa cagattggga tttggataaa
     8521 gatttaggta ctacttattg ggaagtcaaa gtaaaagacg gcaatcaaag tacatcagtc
     8581 aagatcaata gccaaacagg cgaagtactg tcaactgaac aagatgatta agaatttctt
     8641 cggaaaacaa gagttgattt ttcttttagt catgatacac tagacaaaat aacttgtttg
     8701 aaggagcgat ttacttgagt ttagaaacga cctttgccaa tcatatcttt gccaatcctt
     8761 tgatgaatgc atctggtgtc cactgcatga ctacccagga attagatgaa ctggctcatt
     8821 cagaagcagg agcatttatc actaagagct gtacgatcaa tgaacgaaaa ggaaatcccg
     8881 aaccacgcta ttttgatgtt cctttaggga gtatcaattc tatgggactt ccaaacttag
     8941 gtttttctta ttatctagag tatgctcttg cgtatgaaaa agtgcaggaa aaccaaaatc
     9001 aaccgttatt tttttccatt gccgggatga gtgtccagga aaacttggaa atgcttgaga
     9061 aaatagaaaa aagtgggttt aatgggatca ctgaattaaa tttatcttgt cccaatgtgc
     9121 ctggaaaacc acaactagct tacgattttg aggccactta tgaaacactg aaagaagtat
     9181 tcagcatctt ttctaagccg ctaggaatca aattgcctcc ttattttgat tttgctcatt
     9241 ttgatcagat ggctgatatc ttgaatcagt ttccattgac ctatgtcaat gctatcaaca
     9301 gtgtgggcaa tggtttgtat atcgatacag aacaagaagc agtagtcatc aaaccaaaag
     9361 aagggttcgg cggcattggc ggagagtata tcaagccgac tgctttggcg aatgttcgcg
     9421 ctttttatac acgtttgaaa ccagaaatcc aaatcattgg tacaggtggg attcggacag
     9481 gacaagatgc atttgaacat ctgctatgtg gcgcaagcat gttacagatt ggtacagagc
     9541 ttcataaaga agggccggaa attttttcta gaatcataaa agaattaacg cagattatgt
     9601 cagaaaaagg atatacgtca attgacgaat ttaaaggcaa attacgcacc atttcatagc
     9661 aaagagacga aggtcatgat tgatcttcgt ctttttttat aaaaaacact cgtctgttca
     9721 ttcctgtgca agcgtactca ttgtatgtta gacgaacttg tgggaaaata acagaggcaa
     9781 aaaggaggat aatatgaatg atgcaacaga attagcaaaa gagcttaaag aaaaagaatt
     9841 tagttttcaa gaactagtta cggatataca gaaaaaaata aagaagcaaa atcatgaatt
     9901 gaatgcgttt gtcgcgcttg aaccattcga gagtaattcc cacgagctat tgattcccca
     9961 gtcagaaaaa gggccattgg ctggtatccc atttccttta aaaatgctcg gtcaagaaaa
    10021 aaagggatgg ctagctactt caggatctcg tttatttgaa acacaccgag cttctcatac
    10081 atctaattat gtgcggcaag cagaagcgat aggtctagtt ccttttggac aaaccaatgc
    10141 gccagagttt ggtttcaaaa atattacaga cccagtcatc tatggcccag caagaaaccc
    10201 ttggaacctt gaatgtacac cgggcggatc aagtggagga gctgctgctg cagtttcatc
    10261 aggaatcgtt cctttagctg gtgctagtga tggcggtggt tctattagaa tacccgcttc
    10321 gttttgcgga ttgattgggc tgaaaccttc gcgagggacg atgccagttg gtccatatgc
    10381 ttggagagga tggcaaggag ctgctattga tttcggcttg acagtttcta tgagagacac
    10441 ggaggcttta ttttatggga tgagaagtat ccattccggt gcgccatatc aagtatcgcc
    10501 agttgagtgg caaacacatc caagaaaaaa acggttgaag atagcttttt gtgttgattc
    10561 accgatccac tcacagatta gtgaagaggc agtacttgct gtaaaaaaag ctgtagattt
    10621 ccttggaaaa gaagggcatg atttgacaga gattccttac ccactagatg gtcgtaaact
    10681 gatcacgtct tattatcaga tgaatggcgc agaaacagca gcaatgatcc gctcgattga
    10741 aacaggcatc cagcgagctg tcagaaaaga agaacttgaa cccatttctt ggactttgtt
    10801 ccaatatggt cagaagctct ctgccgctac ttacatcgat tctctgcatg tatgggatga
    10861 agcagcaatt gtcatggaaa acctacatca agaatacgat ctattccttt caccaacgac
    10921 tgcatttacc gcccctaaaa tcagcgaaga tttgcaaagt gatgcgataa gagcacagat
    10981 ggaacacatc agtgattgtt ctgaagtaga aggtttagac gttgtttctt ccatgtttga
    11041 ggagagcttg aagatcacac cttatacaca gttggctaat ctgactggac aaccagcaat
    11101 cagcttgcct actcatttga caaaagaaaa catgcctcta ggaatccaat ttatggctgc
    11161 cagaggaaga gaagatttgc tatttcaaat cggctatcta tttgaagcaa atcatcagtt
    11221 ccatttacca aatccatgga aatagcagaa acaaggagaa gaagaatgaa gaaaaaaaga
    11281 ttgtttgctc taataactag tgcctttttt attttaagcg cttgcggtac agctgccgct
    11341 aatcaggaag ataacagctg gagtaatgta gaaaaaaaga aagaattagt agtagcgact
    11401 tctggcacac tgtttcccgc atcttattat aatgatcaaa atgaattagt tggttatgat
    11461 gtagatttgg caaaagaggt ggctaaacgg ttaaatgtca aaatctcctt taaagaatac
    11521 aacgtggatg gacaaatcac ttctgtttca aaaagagaat cagattttgc tgcgaacgat
    11581 tttggtatca caaaagaaag agaaaaaaaa ttctcccttt cagagacttt caaatattca
    11641 tttgacagta tgatcgtacg aaaaaaagat gattcaggaa tccactcact tgaagatctt
    11701 aaagggaaaa aagcagctgg cgaacccaat actagctata tgcgtttagc ggaaaaacac
    11761 ggagctaaat tagtcacata tgacaatgcc acgaatgatc agtatttaag cgatgtagca
    11821 aatggtcgaa cagatgttat tctaaatgat tattatctgc agaaaatggc cgtatctgct
    11881 ttaccagata tccctgtaaa aatactggag aacgtttatt tcaatgaaaa cgaaagtggt
    11941 tttttattga aagaaggaaa tgatgctctg aaaaagaaaa tcgatggcgt attaaaagag
    12001 atgaaagaag atgggacgac aaagaaaata tctgaaaaat attttcaaac tgatgtttca
    12061 gtcaaaccga aagaaacgat ggctgagtcg gtgagtgagt gatgtacttt cctctaattg
    12121 atttttccct catgtggact tcacttcctt tcgtactgca aggcttggta tacacattag
    12181 ggattggctt cgtgtcgttt cttttaggaa atttggtagg gttgcttttg acagttttag
    12241 gtcttttgga ttggctgcct ctgaatgtat ttattcgttt ttatttatcg ttctttagag
    12301 gcattcccgc actagtcctg ctgtttctgt tatatttcgg gttaccttat caattaagtg
    12361 ccttaacggc ttcagtcatc tgctttacga ttaccagcag tgcatttatt ggtgaaatct
    12421 atcgaggatc actagcaggc gtttcttcag gtcagtggga cgctggttta gcttctggtt
    12481 tttctttctt tggggtattg aaatatatcg ttttgccgca agcttttcga atctcagtac
    12541 cggcactcag taacgtagct atggatctag tgaaaggtac ttcgttagca gcaacaatca
    12601 cagtgccaga aatatttcaa aaagcaaaga tcataggcgg tcggacattc gattattttt
    12661 ctctctatat tttagttgca ctgatttatt gggtcatttg tatcgggatc gaaaaagtgc
    12721 aacagtattt agaaaaacgt tctatccatc agtcaatctg aaaaatagaa atggtgctag
    12781 cgtggctggc accatttcta ttttttgttt cttgtatatt ctgcctagta ttttaaaacc
    12841 atttcatgta aaatgtaaat cgaaaatatg ttcggaggta gaaaacatga agaccccgct
    12901 aaccatcgaa atagaatcct cgttatacca atattgcatc gagcaaggcg gcttggttgt
    12961 agaagaagtc acgatgcctg atgaccaagg aattgtagat acgcttagct gcttgacaaa
    13021 atttgatgga agccgagaat ggcgatgcta tgagctgaaa gtgacaaaag cagattttcg
    13081 ctctacagcc aaactgtcat ttgttggaca ttataattat tttgttttac caagtaaact
    13141 atatgaagaa gtaaaaactg aaatccctac aggaataggt gttttagtgt atcgtccgta
    13201 cacggttgaa gaggagatgc ctgcctctgg tacatttctg attgcgaaaa aagcagcaaa
    13261 gaaagagctt gctgtaccgg aagaagcttt gacaaaccgc ttcatggctt ctttattcag
    13321 ggaagtaaga aaagcaaagc aaatggagcg aggcgtaaaa tattttccaa aagatcaatt
    13381 gtacaaagaa ttgaaaagac gttcagaaaa tcaagatcca ttttctaacg aacgatatta
    13441 tgaacggttt ttagaggaaa tagctagtca ggcaatcaat gagttgcaag aagaagttat
    13501 cgcgcttcag catgattatg aatatttgag gcaacaacag cagatccgtc ggttgcctac
    13561 agagccattg gagtgaaaaa tatgtactat ccttatttaa gaggaaaaca atttgaccta
    13621 ttagcattaa aagaagcact gtcgcgagga ttgctctcca ataaaatcca accagtgatc
    13681 gaaccagtac gtgattccgc tacgcttaaa aatgtgatcg aattatttca aaaaaagcac
    13741 catccaatcg ctgtgatcca aaatccccaa gtaggacaat tcaaattatt cgatcagcat
    13801 gtgcacagtt ggacagtcaa agaaaatagc tctgtcgtcc ctgcacagat cttaacgcca
    13861 gaaaatctca gtgaagtact ggattcacct ccagcgtttc ttgttttcga tggacagcac
    13921 tacccaaaag atacagaggt gtggaaacag ttggcaggag ttgattcgaa atttttgata
    13981 ccagatacta gtcgctttcg catctggctg cccgaaaaca aaattgtgat ccgtgactct
    14041 tttcaaacga gaaagcatgt tgaaagttat gcagataaaa cagatgactt ttttagtgat
    14101 gactatcttt ttttccacgc agacggatat attggatttt ctgatttcac gatcgaaggt
    14161 agccgttatt tcgataaagg gttccctagc cgagcgcttg ctatccatct aacgtatatt
    14221 gatgcttacg gcaatatacg cgtgaaacat tttgtctctg acagtaacga ctctgctaaa
    14281 gaccaagcat tgaagtttct tgaagcagga gataaaatga aaaaatggat catgcgtcac
    14341 catcatcaac tcttgattac ttcaggaatg attgaattat tggctcttta ccagcaacaa
    14401 aaattccctg gactgggagt gttgaaaaag tggtcactta tgcatcattt ggaacttatt
    14461 agtcacctgc tagatcatcc aaaagattgg cttaaatatt attcaaaagt tcaggaacta
    14521 tatgaagtga tccaatgatt agataatgta aatgaaaaag gaatagaaaa gaggaaactg
    14581 gatgtatatt cggcaaacaa ggaaagaaga agtagaagaa gtggtagaaa tcattaatgc
    14641 cgcagcggat ttcattgctt cgcaaggctc tcctcaatgg caaaacgggc agaaaccaac
    14701 aaaagaaacg attttgaacg atattgaaag aaaagaaagc tatgtattaa tagtggaaga
    14761 tcaaattgct ggaacagctg cattagtctc aggcgtggat cccgtttaca caaaaataga
    14821 tggtagttgg aaagaatcaa ctggtcctta tttatcgatt catcgtgtcg ctttagcaga
    14881 aagtactcga ggaaaaaaac tagcaaaacc gttcttagaa catttgttga cgattgcagc
    14941 cagcaaaaat atccaagata tccgtatcga tacttatcca gctaatgaac cgatggaaaa
    15001 gaccatctat tctgctggat tcacttatca agggatgatt gaatttcctt ttgaacacgg
    15061 cgaaagaaaa gcatatcaaa taatcatcaa ttgactataa aatcatctga gcaaagataa
    15121 agacattaac actactgaaa aaaacaatca tatgatagcc aagaggttgt aacagaagtg
    15181 gccagcttca aaaaacaaga aagaatttcc gaaaatgctt ctcaaatttt tgtggaattt
    15241 tagcgtattt tacgaaatag ctgctttctg aaactccctt catcgagagg tcgtgacata
    15301 gcttttgtca cgatctctcg gcttatccta cgagtattat tcgatacatt tatatcaata
    15361 atcctttttt tattttatct gcagcttctt ggaacgctcc atcgtttgac acgactaaaa
    15421 gtgaggggat agctttcaaa cgtttcaact gtgctaaatc ttctacataa accgccagtc
    15481 tggctgaaat ctgttcagat gaatctccac gttttttcag gcgcgattgc aatgtttctt
    15541 tagagatcgt aataaagata ggacggacta ccgtcccgaa atactcatat aaatgccaaa
    15601 aaccatttgc tgtcaaaacg gcgtaacaat ttcctactgt cagtttcttt ttaatttctg
    15661 ctttactgga accatattta tgttgagcat attggtccca ctctgcaaac tcatttccct
    15721 tgatcatctg attgaatgta ttgtcagaaa caaaataata atcctcttga tctttttccc
    15781 caggtcttgg tgatcgattc gtgtaagtaa tgatcttttg ttccggctga aaaatagctt
    15841 ctgcgagtga agtttttcca gaaccagaag gaccgataaa tacatagcaa taattcattg
    15901 tcatttctcc tttgatgatt gaacaagtga aaaagctttg gaaaatattt ccaaagcttt
    15961 taaaagtaac tttgcttttt tgtattttat accagcaagc cagtcacaat agcacttaaa
    16021 aagctgacta aagtcgctgt tagcaaaatt ttcaatccgt gttttgcgac aagatttcct
    16081 ttttcttcat ttaatccctt catcgcacca gaaatgatcc cgatagaaga gaagttggca
    16141 aatgaaacga ggaagacaga tataattgct gttgtacgct cagtaaaatg gaaattacca
    16201 tttgctaatt ctgtcatagc aacaaattca tttgtaacca gtttagtcgc catgatacta
    16261 ccggcatcca ctgcttcttt ccatgggatt ccgctgatga aggcaagagg ggcaaagatg
    16321 taacctagga tctcttggaa agtgatgccg aatatcccgt ggaagatagc attgatcatg
    16381 gcaatcaaag caacaaaacc gataagcata gcagcaaccg tgatcgccac atggaaacca
    16441 tcaagaatgt attctcccaa catttggaaa aatgtctgtt ctttgttttc ttcaatgatc
    16501 agctcatctt ctttttcttc taatacatag ggatttacaa ttgaagccag aatataaccg
    16561 ccaaataagt tcaaaacaag ggcagtgatc acatatttag aatcgatcag tgccatataa
    16621 gatccgacga tcgacataga aactgtagac atagccgata cactcatggt gaacaaacgt
    16681 ttctcagata ggaagggtaa ttcctttttt atggaaataa aaacttccga ttgtcctaaa
    16741 atagcagaag ctactccatt ataagattcc aatttgccca tcccgttgat tttacttaat
    16801 cctagaccaa cagctttcat aaataatgga agaatcttga tatagcgtag aataccgata
    16861 atagcagaaa taaatacaat tggcatcaat acggaaagaa agaaaggtgc acttccttta
    16921 tttgctacac cggcaaaaac aaagtcaaca ccttgcccag caaaagcaag aagatgatca
    16981 aacatttttg ccaatacaga aacaaccgct gtaccaaaag ttgtacgtaa taatacgaaa
    17041 ccaagaacaa gctgtaaaac gaacatcaca attagtggtt tcaatttgat tgcttttttg
    17101 ttactgctga tcaaaaaagc aagccctaat gtgacaaata aaccaataat acctaataaa
    17161 taactcaaat aatcactcct attccaaatt ccgttttatt ttacctgttt tgcaaataaa
    17221 atactaatct ttagtgaaat tttcattact tttttagagt gaaacgcatt cattttccat
    17281 tagaaaataa agcgaacaaa ataaaaggtt taaaaagtgt aacgtcttag taaagtctat
    17341 aaaaaaacga aactatcgat taagctaata gtttcgtttt caacatttat aagtaaactt
    17401 tttctattgg taatatagat tttcggaagg gtagacaggt gcgcaggtaa tatcgattcc
    17461 tagttttttc aatgtttgtt catcattttt attgaccatc acagtagagt gggcttgtgc
    17521 tccctcaagt tcagctagtt tatcataagc aacttgagcg attggattcg ttactgcact
    17581 gattgataaa gcgatcaaga cttcattgat gcttagtgca tccaaagagg aatgaagtgt
    17641 ctttgatttc aatccttgta ttgtttcaag aactaatggt gacaataaag gaatgtcatc
    17701 tgcgatatgg gctaaatgtt ttactgcatt taagataact gccgctgacg catccatcaa
    17761 tgaacttgta cgcccggtaa tgatgacttc atctggtaat tcgattgcaa tcacagcaga
    17821 aggatctttt gtaggacttt gcgcttgaat cttatctgca tagtcatggg cagggactac
    17881 cacgtttcgg tcttcaggac gtaaaccgac ttcttccata atcagtttga ttcgattaac
    17941 tgcttcttcg tcgatcaatc cttttttgaa gtcgcattct gtttggaaat aacgacggat
    18001 gatttcttgt ttagaggctt cttggacaac ggtatcatcc acgatcccaa atcctacacg
    18061 attcacaccc atgtcagtag gagattgata aacagattct ttaccagtaa ttttttcaat
    18121 aatacgtttg attacaggaa atgtctcaac atcccgatta tagttcacag ctactttatt
    18181 gtaggcatca aagtggaagg aatcgatcat attgacatct tttagatcga ctgtggctgc
    18241 ttcataagca atgttcagcg gatgtttcaa cggaacattc catactggga aagtttcgaa
    18301 cttggagtaa ccagctgcat gtcctttttg gctttcatga tacagttggt tcaagcaagt
    18361 agcgagcttt ccacttcctg ggccaggagc tgtcactaca acgattggtt tagttgtttc
    18421 aatatactca ttttttccaa aaccatcttc tgagacgatt ttttctacgt tgactggata
    18481 gtcttcgatt tctgagtgtt tatagacctt gatgtttcgt ctttctagtt tattgataaa
    18541 gactttcgtg gcaggctgtc cactatagcg agtgataacg acactgttga ttaataagcc
    18601 ataaccacgt aattcatcga ttaaccgcaa gatatccata tcataagtaa tcccataatc
    18661 accacggatc ttattacgtt caatatctcc agcatagacg caaatcagta tttcagcttg
    18721 atctttcaat ttttgcagca atttgatttt cgcatcttct tcaaatcctg gaagtacacg
    18781 tttcgcatgc ttgtcgtcaa ctaacttccc accaaattct aaatataatt tgtcatagtg
    18841 gttcactcgt tccaaaatat acgctgactg ctcttcaata tatttttctg aatcaaaacc
    18901 aatttttttc actaggttcc ctccttaagt actaaagcat tttttcattt ttttgaggat
    18961 tcgtcaactg gaagagcgaa attcctcaaa aaaacaaaga aaatggttta aaccaacttg
    19021 tctagatagt tattcattgg tatgatagtt tttgataagg aaggtgctac atgaaaaaaa
    19081 gacagaaaaa gaaaaacgca tataaacact atatccgttc gatttttacc ggttatgaaa
    19141 gaatgcttga agatcccgaa ttggaacaat tgacgtttac ttatctaaac gaagaaacgc
    19201 agctaactcg tgatgagcat cagcgcattc atttcacaac aagagatctg cctagtaaat
    19261 aaaaaataag ataaaggtca tcggattcga ttctgtccta tcagatggaa agactagttg
    19321 atcgaaaaaa aggctgtacc aaattttggt gcagcctgtt ttatggagca ttgcccgctc
    19381 ttttttataa caatttttta cagaaaatgg tactattgaa gtattaataa gtgaaaaaca
    19441 gatgagtaag aggaggcaag tagtatgaaa gagccagata caaatgcaaa ttatgagcca
    19501 gacgaactag aggagacgaa cgcggatcag gatacaggac aagaatcgtc ttctttttac
    19561 gatatcaaag aaaaagcaag catgaatctt tcccaattat tagaagactt acaggctttt
    19621 gaaaaagctg tccgcgaaga agatatccct gagatctacc gtttatacaa tggccgattg
    19681 aatgaagaat taaaaagaag ctcaaatgaa aatcacgaga tcgatcaatt gctgatgcgt
    19741 aagatccatg atcgtttttt acaggaattt ccttttatgg aacaagtaga aaacatctcg
    19801 ccaacgatga gctattacaa aatcggcact tattaccatg atcggcccac ggtcggtatt
    19861 gatgcaagtc tgccagaaat ttttgttttg cctaagatcg atgaagagtg ggagaaatat
    19921 tcccaaccgg agtcaacgga ctatgacaaa aaaatcaatg agctagacgc aaaggtgatc
    19981 actgctcaga cagaaattga acgtttagat gaacaaataa aagaaatcaa tcgacaaatg
    20041 actgatttga acgacaataa aggattcttg aatcgtaaaa aaatcgagga agaaattcaa
    20101 gaattagaga agaagaagca agtactatct aatgaaaaat taggctggct tccttatatt
    20161 gagacgcctg aaacaatcca gcagcaaaaa gaagcattga agcaagaagc aagagcagac
    20221 cagcttcgtg cagccatcgt agaaaaagaa caaaggcaaa tcagacgcta tttcggtagc
    20281 aaagagggat tcggtcaagc aatccatgaa ttcttgatga attatcttgg caatgagaat
    20341 cctaataatc aatctaatga aggaggttcc gagtatgaat gaacacgatg aagcagaatg
    20401 gacaaaaaat gaagaacaaa cagaagatca tgaagaaacg aacacttcat tggaagaaca
    20461 aacaagtaat cctctggatg acacagaaga aatccctatg ctagaggaaa caaaaataca
    20521 tgaaaaagaa aaagtaactc cgacgaatac gaatactgca gaaaacaccc atgcttacga
    20581 aataactgaa aatcccgggt atttagaagg aaaagagcaa gatcatcaag atatcgtcag
    20641 tttgattgaa acagcggaag cagaattagc ggatttacgt atgagaaaag atttgatcga
    20701 atcagatttt ccacaattat tagaatcata tgatcgtttt atattgaatg aagaaccaat
    20761 ctccacgatc ggaaagacaa gcctgatcga atacttcacg tatgaattac tgaaaccgtt
    20821 gaatgatatt ggtctggaac ggtcaaattc agcttatgac acatggaaaa caagacattt
    20881 ttcgatcaat tatacgttga atggacaaaa cgaattagtc ttttcctttg tattaccgac
    20941 catcgatcaa cgataccaag tggaatcgat gagtctccta aacgtatcac cagagacaat
    21001 ggaaatcaat gtccaagatg accgggtgct ttctttgatc cgacactggt cagtggatcg
    21061 agtattctct gcagggcaga tcacgatatt caatcacaaa ttgaatcaat tattagccca
    21121 tgcaagaaca cttggtttta ccgttaatca gacattacta gacaatacga agccacttcg
    21181 tttgaatctt cagagcgaat ttgaattgac gaatgaagta ttggatgata tttttatcgt
    21241 ggcaatgaaa aatccatcct acgatatgga aaagatcaac gaaactactt atcaagtacg
    21301 attagataaa ggacagtctt taacgataac aacaaacgaa aaagatcaaa cagtcttgtc
    21361 gatctcatct ggagactacc cacgatctgt cattgatttc tttatcaatt acgaattctt
    21421 ggtcccatta atggtgagaa agtcctgatt ggataagttg gattaccaga tgtcattcag
    21481 ataattctgt tccaaaggac ttgcggcagt tatccagaat aattgtcaca agtcttttct
    21541 gtatgtacta aatggacgtg aataacttgc tgatgaagga gggatttcat taggaatgtc
    21601 cacaaataaa atagcacagc ttagcttatt gtctgctgct tgtgtagcag gtaggatcgc
    21661 ttttcaattt attccgaatt tccagccaat gactgctatt tttttattta tagtacttta
    21721 cttaagctta aaagatgcgt taattgtcat gagtctttct attgcgatca gcagttttta
    21781 ttttggtgtt ggaccatggg tcatcggcca atggatcagc tatactgttg ttctgagttt
    21841 gttttcaatc gtctgcttga gaaaaacagt gcaaaggaat ctatggttaa agggagtctg
    21901 tttttttctg gcgggcatgg tttacggaat aggtatgacc gtttttgata cgctgttata
    21961 tcgtttgcct caaccttgga tctattacct tcaaggggtg tcgtttgatt tgatgcacag
    22021 tataggcaat gttgtgttct ttcttgtttt tctgccagtc gtcaaacgtt tttacaatca
    22081 ttcaggagga aaaaatgaaa aaaataattt atagtttgct tgcaatgagt gtcagtatac
    22141 tcattttgac aagttgcagt acaacctata cacaatcggc ttccaaccaa aaggaggaag
    22201 taaaaacatc gcaaaaagca acaatcacgt tacaagaaaa tggtcaaaac ttttcggaaa
    22261 aagaagtggt cttcaaaaaa ggcgatgatt tactgagtat tttgaaacga aattttgaag
    22321 tcaaagaaga taacggcttc atcacttcgc ttgaagggca ctcacaggat gagaaaaatc
    22381 aactctattg gatgtttact gtggacgggg aaagtgctac tactggtgcc aaagagatca
    22441 aattgaatga tggacaatcg gtcattttca atttgtctaa actataagtt tcataaaaaa
    22501 ctgttaaagg gcgaatattg ccataattca gtgagttcag ttgatgaaag aaggaaaata
    22561 acatggaaaa ttttaaaaaa agcggaatgt tttccgtaat tgctgctcta ggggctaata
    22621 ttctagttgc tatcagcaaa tttgtcggct atgcaatcag tggcagtgcc gccatgctaa
    22681 acgaaagcat ccacagtatc gttgattgca gcaatcagat tttgctgtta ttcggtgata
    22741 aacaagccaa taaaggacag agcgagttgc atcaatttgg tgaaggacga gcaaaatact
    22801 ttttcagtac aatcgtcgca atgatgcttt tcttcggcgg tggggcactt ggggtgatgg
    22861 aagcttttga aaagttgacg catccatccc atgaagtggg taatccgatc attgttatag
    22921 caatactgtt atttggtttg gtaatcgaag gaagttcgtt acgcgtggca atgaaagaaa
    22981 tcaaagaatt gaatactgaa aaactttcca cgatgcgctt tttgcgcgaa agtcgacaca
    23041 gtgaaatact gattattttt acggaagact tgtgtgctgt aatcggtttg gtcttggctt
    23101 tagcaggaac aatattgact tatgcaaccg ggattgctgc atttgatgcg atcagcggat
    23161 tgctgatcgg tttcttgctg atgggagccg ccattttcct agcaaaagaa ttttatagtc
    23221 tgatcatcgg tgaaagcgtg acagcaagcg atttatcaaa aatcaagtta gcttttgaac
    23281 gtcctgagat cagtcggctg atcgatataa aaacagttca tttaagtcca acggagattt
    23341 tagttgcagc aaaagcagat atcgttggta acaaagagaa tgaagcttac tcggtcatca
    23401 atgatattga aaaaaatatg cgtcagtctc ttcctgacaa gaagatgtat atatacattg
    23461 aaacagataa atatgaccca aattattctc gtaaataaaa cgtttattca tgaattgaag
    23521 caaaaatgag aaaaatataa tcttgttttt aaataaaacg gctacaattt agcgaattta
    23581 tgttacaatg agtccgctgt ttatttgttg taattaaagg agatatttta atgaacaaaa
    23641 agaaaagtgc gccatggctt caagctttgc atgcagtgat gccactttgt ataagctata
    23701 taccagtagg attagcttgc ggagtattgt tgcaaaaagt tggatttgat cctctcttat
    23761 caggactgtt atcgattctg gtcttttccg gtggcgcgca gtttttagtt gcatctatgc
    23821 ttacgacgca agcatcattt gcaacgacat tattgatggt tttcttttta gaattacgct
    23881 atacattatt aggagcaagt cttgccggtt ttatgaaaaa agaaaagcgg acgtttttgg
    23941 ctgtcttttc tcaatcactg aatgatgaaa actatgcggt gaactactta aagttttcaa
    24001 ctgacccttc ctggaataaa agaaaggctt tatatgtcaa ttggttctca atgactgcat
    24061 gggccggtag caatatgatc ggtaattttt tcggctcggt gattcgagta gatgctgacc
    24121 ttgtccattt tgctttgacg gccatgttca ttttcatgtt tatcatgcag atgaaaagcg
    24181 cactactgat ctttacaggt ttattctcag gcgtattagg ggtcgtattc atggtattat
    24241 tccaaaatac tttcggattg attgcagcaa cagtcattgc ttcatttgcc ggatttatgc
    24301 tggagaaggc attcaaaaaa gaaaaagcta agaaactaag agaccgcggt aaagatgtca
    24361 atgagccgct cttcaacttc ccggaacagg aggccaatca ccatgactaa actatatttg
    24421 ctacttttag ttgcttgtct gttcatcgtg gcatatatcc cgcgcttgtt cccgatgctt
    24481 tattttactc atcgaaaagt gccatcctgg ttcagtgact ggatgaaata tgtaccggtg
    24541 gcattatttg cggcgttagc ttttaaagat gttttcatta ctcatgaaca tctggatatt
    24601 gcttggaata tcaaaatagc tgcaatgatt ctagtagcag gcgtggctta caaaacacgt
    24661 tcgatggcgt tgtcagtttt gacaggactt gcgtctgtct ttttgctgtc tatgctttaa
    24721 actgttttat tttattaaaa acagagattg cgacataagt tgttcaaact gatctatccg
    24781 aactgcattt gcaacagtca attctggatg caagtttatt cgtagggata aaaaagatta
    24841 gtgttggctt atgatgcaag ctctttttta ttttaggaat tagagaggga tttgtccttc
    24901 tatgattttt tgctataata gggaagatgt ttattttatg aaagaatagt tgagggaatt
    24961 taaatgaata aaaaattgat gcaaggaaca ttttggctaa cctttgctaa tcttctatgt
    25021 aaagttttag gggtagtcta tttgattccc tggctaagta tgatggggaa caatcaagat
    25081 ggtatgcttg ctacgacatt gtataatgtt ggctatcttc cttatggatt gttcctgatg
    25141 cttggtacag tcggatttcc aaatgcgatt gcaaaaaaag tagctgtagc cacaaaagaa
    25201 ggagataacg ctgcttgtcg gctgattttt agaagtacga tcaatatcat gtttgtcatc
    25261 ggtatcgcct ctgctgcact gatgtattta tttgctcctt tactagccgg aatcagtccg
    25321 attgctaacg ttaataatgg gatactagct atcagaagtt tatgcccatc tttgattgct
    25381 atcccaattc ttagtgcaat gagaggatat ttccaaggaa aaaatgatct gcggccttat
    25441 ggaacttctt tgattatcga acaggctgtg cgagtgatcg ttatcttggc tggtacttat
    25501 tacttgcgcg tattgactga cggaaccata ttagaagctg tattgatcag tacagtcgct
    25561 tctttcttcg gtgggttagc tgcgattatc catatgtata tcgtcggacg cagaaaagac
    25621 ttcttcgaac ttaaagattt tttgatttcc agccgttatt tcgaaagaga aaaccgatct
    25681 gcctctgtct ctatcattcg ggaaacacta ccgtttattt ttgtcggctc agtcattact
    25741 atcgttcaaa tgatcgacca agtaacgatg aaaccactgc ttcacttttt ccgtccagag
    25801 atcgcagatc agcaactgga attcttattc agccgtgctt ctgtcaatcc aaataaattg
    25861 acattgatcc tgatctccat ggtaggaaca gtagctataa gcagcttgcc aatattaagc
    25921 acgatgaaga aaaaagatcg gatacaaatc gaaaaaacag taggagatag tttttcgatt
    25981 gcgttattga ttcttcttcc ttcattgact ggaatgtctt tattagctgg accactatac
    26041 acattgtttt ttggctatga tcctgaaagt gtcggatatt tccaaatggc tttgctagct
    26101 tctttgtttt tctctttatt cacgatccta tctacgatgc ttcaatcttt gaatcaccat
    26161 cgttttgcga tccgattaac tgtagaagca attatcctga aagtcatctt ccaagtaatc
    26221 ggattaggat tagttggcgg atacgggatg agcttatcaa atggcttagc attcggtatt
    26281 gtctttgttc gcggctatca ctttatgtgc aaagaatacc gaatagcacc tttagcaaag
    26341 atcagcaatt ttttcttaaa aactttccgc tccaccctga ttatgcttgc agtatgtttt
    26401 gccgttttct ttttgctgaa tcagcgattg tcaatggaat caaaaagcta tgcaatgatt
    26461 tattgtgtgg ctgtaggaag tattggcggc ttagtatttt tattttctca attcggaaaa
    26521 aatgggtttc gaatgttgaa aaatgttaaa catcatagca gaagcaaaga ataggttaac
    26581 taatcgtttt ttgctgtcac ttgaaaaacg gcaactcgta tgattacgct tgtcgttttt
    26641 ctattttcca attttagttt catatctaat cttacttatg atatagttat aaggtctgga
    26701 aaaattcttt gaaacgagga aattaaacac atgaaacttg cagtagttac agatagttcg
    26761 gcatatctgc cggaacgtat caagaaccat cccgacttat tcgttattcc aatccctttg
    26821 attatggatg ggaaaattta taacgaagga atcgatatcg aagcggatga atattatgga
    26881 cttttaaaaa acagtaaaga ttttcctact acttcacaac ctgtcgtagg ggaagttttg
    26941 ggtttatatg aggaattgaa agagaaaggg tatgacacga ttttaagtat ccatctttct
    27001 tcagggattt ctggctttgt caacacatta tttgcgatga aagatgacat cagcggagta
    27061 acggtcattc cttatgactc taagatcact agtatgccga tgggacacat ggtagaagct
    27121 gccttagact taaatgatga aggtcagtcc cttgatgaaa tacttgcaca tatcgatcga
    27181 atccgagata atacctatgc ttatctgatt gtagatgatt tgaacaattt agtacgagga
    27241 ggacgtttga caaatggtgc agcgctgatt ggcagcttat taaaaatcaa accgatcctg
    27301 acattttctg aagggaaaat tgttttattc gaaaaaatcc gttcaagtaa aaaagcattt
    27361 gctcgagctg aagaaattat tggtagaaga aataacgaaa tacaaccgcc agtcaaactt
    27421 tacgtgatac atgcaaataa tttagaagta gcgattgcgg agaaagagaa attgcagaaa
    27481 aaatatccaa atgcagaaat cgaaattggt catttcggac cagtcattgg tacacattta
    27541 ggtgaaaaag ccatcggcat tgccatttca gcgcaataat tccttttatt caaaaaacat
    27601 accaaaaata t
//
LOCUS       DAXWOO010000019        27262 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.019, whole genome shotgun sequence.
ACCESSION   DAXWOO010000019 DAXWOO010000000
VERSION     DAXWOO010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.019"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(<1..248)
                     /locus_tag="ACGE70_000651"
     CDS             complement(<1..248)
                     /locus_tag="ACGE70_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFF8692307.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFTASHFEYSVLYDTSTTFHSKFETEA"
     gene            complement(266..586)
                     /locus_tag="ACGE70_000652"
     CDS             complement(266..586)
                     /locus_tag="ACGE70_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFF8692308.1"
                     /translation="MITFERIKELAKKQGKSLNKVEEDLGYGKNVLYRLKNSNPSTER
                     LQEIADYFDVSVDYLLGREEKETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            880..1020
                     /locus_tag="ACGE70_000653"
     CDS             880..1020
                     /locus_tag="ACGE70_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692309.1"
                     /translation="MEKTVSAKIEDLKLDILKQAKVAMEHAVDKEDSAMVAAIAEILA
                     HV"
     gene            complement(1017..1196)
                     /locus_tag="ACGE70_000654"
     CDS             complement(1017..1196)
                     /locus_tag="ACGE70_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692310.1"
                     /translation="MNKKTDEIAADLVVAWLNHESQVSTKGESISPKEIAQAYLDIHY
                     AVIYGQLPEDRKNDD"
     gene            1277..2023
                     /locus_tag="ACGE70_000655"
     CDS             1277..2023
                     /locus_tag="ACGE70_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFF8692311.1"
                     /translation="MNTPQIFSFEQHEVRTFLENDIPYFVANDVAKTLGYKNPSKATN
                     DHCKKSIETWGNDSLGRRQKFKVIPESDVYRLIIKSNLPSAEKFEAWVMEEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
     gene            2038..2241
                     /locus_tag="ACGE70_000656"
     CDS             2038..2241
                     /locus_tag="ACGE70_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8692312.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            2257..2595
                     /locus_tag="ACGE70_000657"
     CDS             2257..2595
                     /locus_tag="ACGE70_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HFF8692313.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            2582..2761
                     /locus_tag="ACGE70_000658"
     CDS             2582..2761
                     /locus_tag="ACGE70_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692314.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(2804..3274)
                     /locus_tag="ACGE70_000659"
     CDS             complement(2804..3274)
                     /locus_tag="ACGE70_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692315.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            3361..4059
                     /locus_tag="ACGE70_000660"
     CDS             3361..4059
                     /locus_tag="ACGE70_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFF8692316.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            4237..4578
                     /locus_tag="ACGE70_000661"
     CDS             4237..4578
                     /locus_tag="ACGE70_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692317.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            4571..5242
                     /locus_tag="ACGE70_000662"
     CDS             4571..5242
                     /locus_tag="ACGE70_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFF8692318.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            5248..5934
                     /locus_tag="ACGE70_000663"
     CDS             5248..5934
                     /locus_tag="ACGE70_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFF8692319.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            5937..6686
                     /locus_tag="ACGE70_000664"
     CDS             5937..6686
                     /locus_tag="ACGE70_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8692320.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            6698..6967
                     /locus_tag="ACGE70_000665"
     CDS             6698..6967
                     /locus_tag="ACGE70_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692321.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            6972..7136
                     /locus_tag="ACGE70_000666"
     CDS             6972..7136
                     /locus_tag="ACGE70_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692322.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            7129..7431
                     /locus_tag="ACGE70_000667"
     CDS             7129..7431
                     /locus_tag="ACGE70_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692323.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            7428..7589
                     /locus_tag="ACGE70_000668"
     CDS             7428..7589
                     /locus_tag="ACGE70_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFF8692324.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            7586..7891
                     /locus_tag="ACGE70_000669"
     CDS             7586..7891
                     /locus_tag="ACGE70_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692325.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            7891..8247
                     /locus_tag="ACGE70_000670"
     CDS             7891..8247
                     /locus_tag="ACGE70_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HFF8692326.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            8234..8452
                     /locus_tag="ACGE70_000671"
     CDS             8234..8452
                     /locus_tag="ACGE70_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692327.1"
                     /translation="MDLYDIESGATLSVESDHLIINFGGKRQIILWVVDDVLFPEIVH
                     DFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            8449..8868
                     /locus_tag="ACGE70_000672"
     CDS             8449..8868
                     /locus_tag="ACGE70_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFF8692328.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            8865..9422
                     /locus_tag="ACGE70_000673"
     CDS             8865..9422
                     /locus_tag="ACGE70_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFF8692329.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            9419..9715
                     /locus_tag="ACGE70_000674"
     CDS             9419..9715
                     /locus_tag="ACGE70_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692330.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            9792..10205
                     /locus_tag="ACGE70_000675"
     CDS             9792..10205
                     /locus_tag="ACGE70_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFF8692331.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            10514..10666
                     /locus_tag="ACGE70_000676"
     CDS             10514..10666
                     /locus_tag="ACGE70_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692332.1"
                     /translation="MKLSVEGTPEEIQELLQAIGSSKEQKVSVSNSDIDGICNCLNRE
                     KISNPY"
     gene            complement(10663..10938)
                     /locus_tag="ACGE70_000677"
     CDS             complement(10663..10938)
                     /locus_tag="ACGE70_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692333.1"
                     /translation="MYKPYMISYDLNNPGQKYDKVFEIIKEFGAYIKLQKSFWLVKTN
                     LNPNQMCEKLNTVLDNNDSLFICELQKNYQGRATEENWKFINEHIFS"
     gene            11392..11598
                     /locus_tag="ACGE70_000678"
     CDS             11392..11598
                     /locus_tag="ACGE70_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692334.1"
                     /translation="MLGQCFGNHARFDSLPATLNAYDGYDYRKKIVKKLYGATYGNVV
                     SVLSVHHQASVTVAEVEDAAVNGE"
     gene            11794..11961
                     /locus_tag="ACGE70_000679"
     CDS             11794..11961
                     /locus_tag="ACGE70_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFF8692335.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            11987..12331
                     /locus_tag="ACGE70_000680"
     CDS             11987..12331
                     /locus_tag="ACGE70_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFF8692336.1"
                     /translation="MTEEFYRWLLQLTREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            12336..12617
                     /locus_tag="ACGE70_000681"
     CDS             12336..12617
                     /locus_tag="ACGE70_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692337.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            12720..13034
                     /locus_tag="ACGE70_000682"
     CDS             12720..13034
                     /locus_tag="ACGE70_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFF8692338.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            13012..14706
                     /locus_tag="ACGE70_000683"
     CDS             13012..14706
                     /locus_tag="ACGE70_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFF8692339.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            14726..15904
                     /locus_tag="ACGE70_000684"
     CDS             14726..15904
                     /locus_tag="ACGE70_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFF8692340.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            15867..16553
                     /locus_tag="ACGE70_000685"
     CDS             15867..16553
                     /locus_tag="ACGE70_000685"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFF8692341.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            16553..17713
                     /locus_tag="ACGE70_000686"
     CDS             16553..17713
                     /locus_tag="ACGE70_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFF8692342.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            17723..18598
                     /locus_tag="ACGE70_000687"
     CDS             17723..18598
                     /locus_tag="ACGE70_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692343.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFLEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYRKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            18595..18906
                     /locus_tag="ACGE70_000688"
     CDS             18595..18906
                     /locus_tag="ACGE70_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692344.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            18896..19249
                     /locus_tag="ACGE70_000689"
     CDS             18896..19249
                     /locus_tag="ACGE70_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFF8692345.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            19239..19640
                     /locus_tag="ACGE70_000690"
     CDS             19239..19640
                     /locus_tag="ACGE70_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692346.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            19633..20037
                     /locus_tag="ACGE70_000691"
     CDS             19633..20037
                     /locus_tag="ACGE70_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692347.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            20049..20657
                     /locus_tag="ACGE70_000692"
     CDS             20049..20657
                     /locus_tag="ACGE70_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFF8692348.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPNNMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            20677..21039
                     /locus_tag="ACGE70_000693"
     CDS             20677..21039
                     /locus_tag="ACGE70_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692349.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMSM
                     GKGSMDITVIQGAKAVYVAYRQANMVDYISFDEFIDKWDFDMEVAVAVYSTMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            21042..21224
                     /locus_tag="ACGE70_000694"
     CDS             21042..21224
                     /locus_tag="ACGE70_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692350.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            21242..24562
                     /locus_tag="ACGE70_000695"
     CDS             21242..24562
                     /locus_tag="ACGE70_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFF8692351.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNDIDKTSKALAQSKGEFKTYESTMKDLDNEQQHLKSSASLVESEYKKWQ
                     ATAGQSATESEKLAKAQEYVGKQSDIAEQKIEVLKKQLDATQKEFGETSTEALEMKTK
                     LNDAEREFEELSNAAKNVDTSTVDDIGKKLDMGNLMEATDHLSVIGDKLIDVGSKSIE
                     AAGKAQAMQAQFKQVFGSLEGEAQDAVEGMAEEFGMLPNTIKPVFTQYTSMFKGLGYD
                     TKEAMELAGDSTQLAADAAAFYDKSMDDASESLNSFIKGNYEGGEQIGLFANDTQMAA
                     YAVKHNLIPATEGAKKASEESLLAVEKAQSKYADAIKKHGEGSLEAREAALKLKDAQD
                     KINEELGPQTQKWSDLDEATKQAVRVQYAEDMQKLAGATGQASRESDGLENQMTRAKQ
                     ALEDFYASLGEDILPVFIKGLQAGAKALQGLAEWWSKLDGPMKNFILALGGILALLST
                     LAPVITAVVTIVGTFGSTVLLPIIGIIAGVAAVIAIVITAFQNWGAITDWFSDLWKKF
                     TDWLGDTWESIKESASSVWDGVKETWSGFVDWVQEIWQGVSDWFGELWSGLVEGASNI
                     WQGVQETWQAFVDWVSNIWNGVKEVWSIIWADIVGIVQIPWTLITSLIQAGINIIVGI
                     FDVAGQLLGAAWQAVWTPISDFLKNTWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNG
                     IWTSISTTASNIWNSISATASSIWNSIKNTITSLVQAAATVIQNIWSTVSSWLGGIWN
                     SISSTASNIWSSVTSSISNAINAAKSAIQSVWNSISSWISGIWNGIKNTALNLWNGIT
                     STISSKVNDGKNAISSGWSNLTGIVSDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKI
                     SSVFDNLNISIPHIPLPHFKLSGEFNPLKGKIPTLGVDWYAKGSVFNSPNIIGVGEAG
                     PEAVLPLKRSVLQEIGDRILSSTSVSSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQ
                     QIIDSITKVQNDNASAWR"
     gene            24613..25350
                     /locus_tag="ACGE70_000696"
     CDS             24613..25350
                     /locus_tag="ACGE70_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFF8692352.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            25360..>27262
                     /locus_tag="ACGE70_000697"
     CDS             25360..>27262
                     /locus_tag="ACGE70_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFF8692353.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGNSLGIDGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVY"
BASE COUNT         9756 a         4108 c         5709 g         7689 t
ORIGIN      
        1 tctgcttctg tttcaaattt tgagtgaaaa gttgtagaag tatcgtacaa aactgaatat
       61 tcaaaatgag aagcggtgaa atgcgcaagt tcatgagata aatgaaaggc ttctgcagtt
      121 tcgccatata gattttcatt caaaaaaatg attctaggct tcggataata gaagccaggc
      181 tctttcattt ccatgtaaac tactttcaaa ttgtattcgc tcaacatttc tttcaatttc
      241 aaatacatac aacccatcac tccaactatt cattttcctc taaagcttta gcaattgcaa
      301 tcgctttacg cattgtctcc ttagatattt cttttccatc aaaagaaaaa acagtatcgt
      361 tttctgataa atccacatgt ttaggggttt ctttttcttc gcgccccaga agatagtcta
      421 cagagacatc gaaatagtca gcaatttctt gcaaacgttc tgtagagggg tttgagttct
      481 taagtctata taagacgttt ttaccatagc ctaggtcttc ttcaacttta tttaatgatt
      541 ttccctgttt tttcgctaac tcttttattc tctcaaacgt tatcatatca atattccttt
      601 cgttcaagaa aaaatattta actttatttg ttaaaattcg ttgactattt taacaaaagg
      661 tgttaatatc attcttgtaa acaagtttaa caactaaaaa gacaacaaaa aacactattg
      721 attaataaat gccaaccgcc aagaaagctt ttaaaatcaa tgtttatatg tcttatttaa
      781 ctatgttctg attttaacac tgtatgttaa tttagtcaac gcagtttttt taaaagttgt
      841 taaatttgtt tacgaatata aaagaaagga gagaagaata tggaaaaaac agtctcggca
      901 aaaatcgaag atttgaaatt agacatactg aaacaagcaa aagtggcgat ggaacacgcg
      961 gtagataaag aagactctgc catggttgca gccatagcag agattttagc tcacgtttag
     1021 tcatcgtttt tacggtcttc tggaagctga ccatagataa cagcataatg aatatctaaa
     1081 taagcttgag caatttcttt tggcgaaatt gattcgcctt tagtagatac ttgtgattcg
     1141 tgatttagcc atgcaacaac taaatccgca gcgatttcat cagttttttt attcatactt
     1201 attcaccacc ttatgcatta tttcagtaga ccacttactg ataagaaaat tatatcaaag
     1261 aaaggaatga acaaaaatga acacaccaca aatttttagt tttgaacaac atgaagtaag
     1321 aacgtttcta gaaaatgaca ttccgtattt cgtagcaaac gatgttgcca aaactttggg
     1381 atacaaaaat cccagtaaag ccaccaatga tcattgtaaa aaatcaattg aaacatgggg
     1441 taacgattcg ttaggtcgtc gtcaaaaatt caaagttatt ccagaatcag atgtttaccg
     1501 cttgattatt aaatcgaact taccaagcgc tgaaaaattt gaggcctggg taatggaaga
     1561 agtccttcca acaatcagaa aaacaggtag ctattcaaac gtacctcaaa gttttgcaca
     1621 agcattgcgt ttagcagcag atttagaaga aaagaaccaa ttactcgaac aacaaattgc
     1681 cgagtacgaa ccaaagatta gctacttaga tacgattctt tcatcgacag atacggtagc
     1741 tacttctcaa attgcagctg attacggaat gtcggcaatt gctctaaaca aattgctaaa
     1801 cgagttaggt gttcaacata aagttagcgg acaatggata ctttaccgaa aacatatgaa
     1861 ccaaggatac acaaaatcgc acacaagtga gattccgaaa gccgatggcg gcactaaagt
     1921 tgtaatgaat accaaatgga cacagaaagg gcgagtgttt atttacaact tattaatcgc
     1981 agagggctat taccctcaaa tggatttaga ggaaattggt tagaaaggag ttttagtatg
     2041 actgacattg cagaaatcac tcaacgagat agagaaaaaa tcaaagaata tgtcgaaagt
     2101 tcgaagttct taacttacac catgcttgct gaaagatttg gaattagcaa aagctactta
     2161 tctttaattt taaacggtaa aaagacttct gcagaagcaa atagaatcat agattcgatt
     2221 attactatgt acgaattgta aagggaggaa cagctaatgc aatatctaga agcgaaaatc
     2281 ccaattccag aaggctatgt aattatctcc caagtggatt atgaggagtt aaaaaaagct
     2341 gatgatactg gtagatggat gacgttgcca gaagtgctag aacggattaa cagaaaatat
     2401 gattggttta cttctagagt tttaaagaac ccaagatata gaaatattat cgatatcgag
     2461 aaaaacaaaa atggatttgt ctattatcca gttgaaggaa gagacacata tctattttta
     2521 agaagtaaaa cacttgaatt tttagaaaca aatttttcgg aaatcttaag gaggcaagcg
     2581 gatgggaaaa tttaatagag cgctagtgtt cagcgcaccg ctaatcatct acgctttagg
     2641 actttgggga agcaggcaag cgttgatagg aacgattgtt tacatggttt ggatttttat
     2701 ggggcttgat gaagctgaag ctgagtacag agcgaaaaag ccaaccgagg gggctgacta
     2761 aaaatgaata gaaaagagaa actagaatgt atattactat tactcagttt aattctttca
     2821 ctaatttctc tattgggaag tttgtatttt tgatatcaac aattttgtgg atttgggttc
     2881 tatttagagt aaatatcatt tttgtttggt gattaaggag tttattaaaa ttaattttcc
     2941 ctacttcaaa tgcaaggtaa caagatatag cacttttagg aggaatattt agcggtacag
     3001 cttcagagta tattctatta tcatcgactg atcctccttt agcaattaat ttcttaaatg
     3061 gagtagctga ccatctgcgg tttaagttag cggatgaaag ttctaaatta actaacatag
     3121 ctggttctgt tgaatagtta gaaatgatta cttttgtata aatcatatct gcagcgaagt
     3181 aagaagcatt aagttcaact tctagttgag ggcgcattcc ttttattttt atccctgtta
     3241 aaaacgtact caatataaaa cttattattg acatccattg aaaaattgtt aagtgaaaaa
     3301 aattcaaaat aaacaccacc agtttttaac taaattatac caaaaaggag agaagaaata
     3361 atgcaagaat tagtaatttt gaaaaataaa gaagctgtga ctacgagctt acaagtggca
     3421 gaaagcttcg agaaaaaaca tcaacatgtt ttaagagata ttgatgcact aaaaaaagat
     3481 gtgtccaatt ttggacagat gtttgtagaa ggtaatgaac cagattcata tggcagaaat
     3541 cgacgagttt tcttcattag tagagatgga tttttcttgc tggctatggg gtttacagga
     3601 aagaaagcta tctacttcaa acaaaaatac attgaagcat tcaacgaaat ggaagatgtt
     3661 attcgcaaga atactgttcc tcaaacaatt gaagatatga tgatctatca attagaagaa
     3721 atgaaagatg ttaaaaaaga tgtttccatg cttaaagata ctatgcgaat tagcggacaa
     3781 caagagtttg aaattaagca aaaaggaaat atgaaagtta tgggagttct agggggaaaa
     3841 gaaagccgag cttatgaaga aatcagcaaa aaagtattct caaaattttg gtctgaattt
     3901 aaacgtacct tttcaatccc aagatatggc gagttacctc gtaagagatt cgatgatgct
     3961 gtttcattta ttgaaatgtg gttgccagaa actgcgatcc gtatggaaat tgatcaactg
     4021 aacagacaac agagactttt tggtgatgac aatgaataga gctgaagcgc taagaatagg
     4081 gacggcaatt gctaattgct ggtggaaata ctataaacca atcatcctaa gccaacaaca
     4141 tattgacaag caaaaagcat ggcaacaaat aaaaaagtga ctccgccggc aagcaaagag
     4201 tcacaaagaa aacacatcat aaggagattt tagcatatgg aaaaagaact ttccactcta
     4261 gatcaatatt tgattgatcc tgattggggc aagccgaaaa ttgaggaaac aagtggtcga
     4321 aaaatcagac gaaatctttt gacgaatgaa gaactagctt gggatcaaga tgatttaggc
     4381 aaccatgtaa ctatttggga tcatgtttat cttatccatc tatcgaagca ttcgaataaa
     4441 cctgaatata tttacgtcat cgaagatggc ttgattgatg cgctagaaga gtacgacaga
     4501 gataacttga ttgatatctc ttattacgga ccaggtaaga aatacattgc tgaaatggag
     4561 gcagaatttg atgagtgaaa tcaaagggac aacgaacttt gaaaaacttt ttagtcgtaa
     4621 gttaaataaa attctcaaga aaaaaggaaa ttttgattat ttatcttggg ctcacgcgtg
     4681 ggagattatg aaaaagaatg atccacaggc aacggtaact attaatgagt acaaacacta
     4741 cagggttgtt tctggaactc atcaagactt tcttgttgag gaatataaac cttttcttat
     4801 ggatgaaact gggacttatg tatctgtctc agtaacggtt aaaggacaca cggaaaccga
     4861 attatttcct gttttagatt atcgaaacca accagttgtt aaaccaaatg caatgcaaat
     4921 caataactca ttgaagcgat gctttgtgaa agcattggct ctacacggac tgggattata
     4981 tgtatttcaa ggggaagata ttccaacacc acctagaatc gatacaaaga aattaaacat
     5041 gctagagacg attctagaag ctttcaatga gcagatgggt aaagatatga ccaaaacctt
     5101 aatcgaatat gttaatgagc agacagataa attagggctc ttagctgata acgttgaaac
     5161 tattgaacag ttaagctatg agcaatgtgc cttgatggag cgagcaatag cagctaagag
     5221 aaaagaatta gataagaagt gatatgagtg tttaaaccat taatcgattc atattcagcg
     5281 gttctgaaaa agttcaaagg aaaagacata agcgcaacca tcaatgagga agtgaacatt
     5341 gatcgactaa agacgatgta tgacggctac gatggtgatc gagtcattga aattcgtttt
     5401 attgatccta gacgtttcac cgtacagcaa cgaaacttca tctatgcgct gataggcgat
     5461 atttttatcg atacaggcat gccaacggac ttctggaagg aattcttcta ctttcgtttt
     5521 gaaggtgtca cagggcgcaa aataagcctg aaagacgaat cgaatacaac tgtgagtgat
     5581 gccaatgtct tagcaaatat catcttagat ttcatctttg aacatcatat tcctttcaaa
     5641 gaaggctatg agattttacc tgcgaatcaa gaatattact tctacaaatg catcacaaaa
     5701 agagtctgct gcatctgtgg caaaacagga gctgacatcg atcactttga caaagcgcta
     5761 ggaagacgaa agcgcaaaga agttgatcat gcagagtaca catttgcagc actctgcaga
     5821 atccatcaca cagagaagca caaaataggt gtgattaatt tcaaaaataa atatcaaatc
     5881 aaagggatca aattaaacca gaaaacaatc aaaaagttaa atattggagg gtaaaaatga
     5941 cagaacatcg aagttattat gcgattatac cagccaacgt aaggtacgac aaaagactta
     6001 aaccaaatac taagttgtta tacggagaga taacggcctt gtgtaatgaa agaggctttt
     6061 gttgggcagg caatgagtac tttgcagatt tatatggtgt gaataaagag accatatcgc
     6121 gatgggtaag tgatttgatt aagtttggat acttgaatcg ggaaatcatt tacaaagagg
     6181 gtaccaatca aataatcaat aggtacctac gaattaatca ataccctatt gacgaaaaac
     6241 gcaatacccc tattgacgaa aaagtcaaag ataataatac atctattaat aatacattta
     6301 ataatacaaa agaatatata agagagttac cgccttcgaa aaaatcgaag gctaagcccg
     6361 tccgtcataa atacggagag tataaaaatg ttcttttgtc agatgagcag atggagaaac
     6421 tcaaaacaga attccctaat gattaccaag agcgaatcga acgactgtca gagtattgtg
     6481 aatcatctgg taagacttat aaaaactatt tggcaactat tcgaagttgg gcaaggaaag
     6541 aaaaaagtga acctaagaac gcaagcagtg gatacaagcg cacaggaaga cgagagaagc
     6601 ttcctgaatg ggcaatcgac caagaagcct atctcaagaa aaaagcgcta gaacgagcta
     6661 atagacaatc aaaagcacca ttctaagagg tggaaaaatg aagatcgatt atctagaact
     6721 aattaatgaa atagcgaatt ataaaaaggg cgaggaatta gacgtcctga gagacgtata
     6781 tgatcaactc gaagaagctg gaattgaagg aattaagaat gatcgttcga gttggagtaa
     6841 gctcagatac tatttcgcac tttatatcga tacaacacaa ttaagaaatt tagcatatac
     6901 caaattacta tttgttgatt gtgttaaagg cttgcaaaaa catcttaatg aacttgagca
     6961 ggtgtaatca gatggaccta aagacattta cagcacagat tgaactaatg catcaagaag
     7021 ctttaagaca aagtgtatcg tacgaagaca agtggctcaa cacgttccat ggcggacgtg
     7081 agagcgcact tgatcaagta ctcaaattac tgaaaggaga atgtcgggat ggataagaaa
     7141 gcggcaatgc agcgaattat cgaattgact tattcagaag attggcaaaa tgacaaagaa
     7201 gctgcttcag aagtgatgag gcttggaaga gcgatgtggg cagacaagag caacaagcca
     7261 agaccacgaa aaatcgcaat ttggcacggt gacaaacttc tagtgatagg gacagctgaa
     7321 cagttagcaa gtctcacagg cttgcacgag aaaatcgtga ggaaaagagc aaggtgtggc
     7381 tacacagacg ctaagaagag aacgtttaga tacgtggagg aatcatcatg acaacagaag
     7441 aagtgattca aatgcgtatt cgaagccttc agcgtgagat tgacgatctg gaacgaacaa
     7501 aggcagtgat ggtcaatgaa acggcgagaa aggcaatcga tttgcacata gagaatttaa
     7561 gaagggaaat ccatcgattg gaggaatgag cgtggataag aaagcagcaa tgaaaagaat
     7621 tgctgaatta accaagtcag aatcttggca agaagacaaa gaaatagttg cagaagtcca
     7681 aaagctcggt aaatcaatgt ggactgaaaa gcccaaacgg agaacgccga gaaaaattgc
     7741 aatctggcat gatgaccgaa ttttagtaac aggtactgct gaacagttat ctgaaattac
     7801 tggattaagc aaaaacatta tctgggatag agctaggagc ttatggattg attcaaaagg
     7861 acgacagttt aggtatgtgg aggagagata atggatctca ttacacaata cagtgacatc
     7921 atcctcaaga aaatcatgat gaagattcag aaagataaaa aatcaaaaga acgagctgaa
     7981 ttagttaagt tagaaatggc tgaaacagga gcaggagtgc gaagtagcag gcattggaaa
     8041 gcagcagcaa acattgaatt ttattacaac gaaattcaaa aagggttcga tcagatgcgt
     8101 gagctggatc ggcaaacaaa ttggagcaag aaacttcatc aagatcgttt caaatttgta
     8161 gagaagtata aagaaatatt agaagagtat ttgaggagga cagcaaatga taaaaaaact
     8221 cgttcaattc agcatggatt tatatgatat cgaatcagga gcaacactat ctgtggaatc
     8281 ggaccatcta atcataaatt ttggtggaaa gcgccagatt attttgtggg tagttgatga
     8341 tgtactgttt ccagaaattg ttcatgattt cgaagaatca aaagcggttg agtttgaaat
     8401 agtgaaaaaa gtaatggaat tgattgaaaa atacgaggag gacagcgaat gataccgaag
     8461 tttagagcgt gggataaacg aaagaacgta atgagagatg tagccgtctt gcattttact
     8521 aaaaacggca aaacaaactt tattgaatat tggataaatc ctaccgaatt gaaatcatat
     8581 cacgtgcgaa acatcgacct catgcaatcc acaggaatga aagataagaa tggtgtggat
     8641 atatttgaag gggatatagt attagtcagc gtgcgaaatg gcttcgatta cttagataat
     8701 aaagtctgta ttgtcaaaaa ttcaatagat tattccggat tagtttgtgc cactgttgat
     8761 gaagacttag agtatcaaat ttttaacaca gagctgtttg aagaatacac gtacgaagtc
     8821 accggaaata tatacgagaa tagcgagtta ttggaggaac agcgatgaat aaacaggaag
     8881 caatcaaaaa gttagaaagt attaaagcga taggaaatga tgcaatagct gcttgctata
     8941 acgagagtat aaattcaggt attacgttaa tgaaaaaaat agacgaaccg cagaaaccag
     9001 ttattccaca actcgtggcc ggttggcttg agaaatctac ggaccctttt acaaaagctg
     9061 aaaaaatagc gtatttaatc aaatctaaag atggtgattc atattatttc tgtgattggt
     9121 ttgtacgaga tggcatagtg acgcaagagc aaggagaaga attactcgct tgggcaacaa
     9181 gacaatcata tgaaacacta ttgagcctat acaacggcta cgaggttgag aaagagccgt
     9241 tatgggcaat aaagaatgcc gatggaaact atcttactaa atgtgcttta tggggaaaag
     9301 atggagtaaa ttatagtttt gaatgcaatc catctcatcg attgcttttc actgataaag
     9361 caacagcgga tgctgcagca ttgttggtga atggaacagt ggaagaggtg gtagaaagat
     9421 gaaactaaaa gacggatttt acgctagcag tcacggtatc ggcggtttaa tgctagatat
     9481 gccgacaaag aaccctaaaa cacgcaagaa atcaaaattc aaagtcggtg acatggtccg
     9541 ctgtgaagca gaagagttcg tttatccgtt cagaggatac gttaaaaaga tactgtcaaa
     9601 ctcagcaatc attcgtattg aaaatacaat gaagtgtgat cgttcgactg caaaacacaa
     9661 gcattattta gctgtagcga gattagttga catggaagta atcaagagca aataaaaaaa
     9721 gccggatcgc tccgactgat tcaataaatc caacacattt attatatcac ataaaggagc
     9781 ggtttgactt gatgcaattg ttacgagagg tagatttcaa acagacaaga tgtaatgcga
     9841 gagatgtgct gaagaacttt cggcgtttgg agcggatggc aggtcgctct ttgatagata
     9901 ttaagtcgcc gattataacg gatatgccga aggcaccgaa gcatggcaat aaggcagaag
     9961 acgcgattat tcagatgatg gatatagaaa cggagagaga tgcgattcta gcggctttga
    10021 tggctcttag tctgattagt cgtcagatac tctactacag cttctgtgtg ccagatagct
    10081 tctcaaacta cagaattagc cgtgaagtgg gttattcaga aagaagtata caacggatga
    10141 agtcggaagc tctaatagag tttgcagaag catataaaca cggaaaaata attgcttata
    10201 aataatttgg cggttttttg gcggaatgat ggcggttttt agacatttac cagtgttatt
    10261 atggtagtgt cgaaagatta ggaaacagga tcgacaaaat aaacgaaggg aggcaatctc
    10321 cctcatcgtt ttaaacttct tcttagatag acagcaacta attatgaaga aaaggatgtg
    10381 aattcaactc ctcctaaatt cttcatcggt tgctgtttat tttgattaga cagcagcact
    10441 cacaaaacta aacatgttct tgtcgtcttt caactgctgc tgtctattaa tttatgtatt
    10501 ggagggaaga aaaatgaaat tatcagtaga aggcactcca gaagaaatac aagaattgct
    10561 ccaagctatt ggtagtagca aggagcaaaa ggtaagtgtt tctaattcag atattgacgg
    10621 tatttgcaat tgtttaaaca gagaaaaaat atctaatcct tactaagaga aaatatgttc
    10681 gttaatgaat ttccaatttt cctctgtagc tctaccttga taattttttt gcagttcaca
    10741 gatgaataag gaatcgttat tatcaagtac tgtatttaat ttttcacaca tttgatttgg
    10801 attcaaatta gttttaacta accaaaaaga tttttgtagc tttatatatg ccccaaattc
    10861 ttttattatt tcaaatactt tatcatactt ttgccctggg ttattaagat cataactaat
    10921 catgtagggt ttatacatgt tgtcttgcac atccttgttt tatatttcag cggaccactc
    10981 gctgataaat aaaattatac gcttagtatt tattttcaca atattaattt atcgtaataa
    11041 cttttgagta aatagaacaa aaaacctgca ctagtttccg ctagtacagg cagtgactat
    11101 atcggttgat aatctagcat atattaaaaa taattgcaag aaaagaaatt tatagtggtt
    11161 tataccaaat tatcaaagca atgttatttt gttgctgtct attgtttttt aattattcac
    11221 acgataacta aaggtgggtg aagagaaatg attccattaa taatttcaat ttttgcgctc
    11281 tgtcttaatg tctatatgat tggatttaaa aatgggcaaa ataaaaaata gtagcagcca
    11341 agaataattt tatagtgtca ctgtggcgga aagggtagac gctaagcatg tgtgctaggt
    11401 caatgcttcg gcaaccatgc aaggttcgat tccttgccag cgacattaaa tgcctatgac
    11461 ggttacgact accgaaaaaa gatcgttaag aagctatacg gtgctacgta cggcaatgta
    11521 gtaagtgtgc tatctgtaca ccaccaagct tcggtcactg tggcggaagt agaagacgca
    11581 gcggtaaatg gcgagtagcc tcgtgagagc ctggtaagtt ctcgtgagtg gtgcaatccc
    11641 actccagcga ctttaagcaa ccgaggcatc ggcggtttaa aaatataggg gtgcgcaatt
    11701 tcgtacgcgt tttgtgcatc gtgcaagtta ctattacata ttagatcact ctttgagtgg
    11761 tctttttatt tttgcacaaa ggaggaaaca acaatgtata gaccacaata cttagaacag
    11821 aagtatgaag taatcactgt tcataaaggt aatggcgaaa aagtatatga gtatagaaga
    11881 ccaataaaga gcgatacata taaacgaaag gaaaacaatg aagttattcc attgtatggc
    11941 aaaagaatag ctaagcatta aataagattg cgaaaggaga cggaacatga ccgaggaatt
    12001 ctatagatgg ctattacagt tgacaagaga agatcgtttg gttaagttct atcagtctcc
    12061 taaatggcgc aggcttagag agaaagcgat gaaacgagat cactatgaat gccaagagtg
    12121 tagaagacta ggtaagtatc atagagtaga gaacgttcat catataaagg aagtcaagga
    12181 tagacctgac ttagctttag atttagataa tcttatttgt ttatgtgttg aacatcataa
    12241 tgaagttcat ggcagatatc ttacagcatt agataaacaa gagaagaaga tagaaagctt
    12301 tgctaacttc gatgcaagtg aaaggtggta agtgcatgat catcaatgat aatggcagag
    12361 agtatgatac agaaaagatt gaagagtatt catcttatac tcagggatta attaaacgtt
    12421 tgatatacgt tcgctatgta ggtattaggg atctgttatc agataactgt tgtagtaaat
    12481 acaaagtgaa tcaagtaaga gaagcgttga ataaagataa taacgtcgaa agaataaaaa
    12541 atgtttttgg atatagtatt gaagagatta attattacat tgacttcgct gaagctttca
    12601 ttccgatggt gagataaccc ccccttaaaa taaatcgcaa attttttggg ggtgatgaaa
    12661 cggagggggc tgtcaggaaa agagattttt tcgaacttta tcatgaaagg agggctaaaa
    12721 tgtttaaaaa cgaattgtct caaaatcgct acagagaaaa attacgccgc tctttaataa
    12781 gccaattgga aagtcagaaa acaaatattg agccattctt agataatgtt gatcgttata
    12841 tcagtttatg ggaaacggcg atatcactgg aagaagatat atccgagaac ggcattagac
    12901 tggagaatgg taaaaagaat gaatcagtag cgttgcttgt ttctgtcaac aaacaaatgg
    12961 gattgatgtt ggataaactt gccattactc ctgaattggt aggtgaagca aatgaatcaa
    13021 ttcctgagtt ataagcatat tgaaaattgg ttcaaagcta tagaagaagg cactatcaag
    13081 gtatgcaaag agcaattatt gctaaaaaat tatctagaag aaagagtctt tactagagaa
    13141 gatatttact tcgataagca gatggtagag gattcaatca atataccagc acaatacttt
    13201 ccattcgaat taattccgtg ggaaaaattt ctacaatgtt ttatttatgg tgttcgatgg
    13261 aaaaaagata aaacactagt gttcaataga tatctttcat taatgggacg tggtaatggt
    13321 aaaactggtt ttgcttcttg gaacaacttc tttctactaa ccgctaaaca cggtattaaa
    13381 aattatgata ttgatatcta tgccaataat gaaagccaag caaagactag ttttgatgat
    13441 gtatttaaag taattaaaga tcatcctgat ttagataaaa aagtatttaa agctacgaag
    13501 gaagttattc aaaatatcgc tacaaacagc aaacttcgtt ataacacggc aaatgctaga
    13561 acaaaagatg ggaagcgacc aggtgcaaac cgctttgatg aaattcacga aaatgaagat
    13621 tattcaatga taaatgtggc tacttctggt ggtggtaaaa ttcgagatta tagagaattt
    13681 tatgatacaa ctaatggtca tgttcgtggt ggtccgcttg atgacattat agaagaatca
    13741 aaaatgattc tttctggaga acttggaatt gacaaggatg gagcagaatt ttctagtttg
    13801 tttccattta tttgtcgctt ggataacgat aatgaagttg atgatcccga catgtgggaa
    13861 aaagcttgtc caactattaa ttacaatgca gatttaaaac ggaaaatgtt tcaagaatac
    13921 tctcaaatgc aacgtaatgc tggtttaaga cttacgttca tgaccaaacg aatgaacaga
    13981 cctatggaag atacacgatt tgctgttgct tcatatgatg atgttctgca tacgaaagaa
    14041 aaagaatttc ctgaaaaaat ggatgaagtg ataggaacag tcgattttgc tgatagacga
    14101 gattttgcca gcgttgggtt gctaggaaaa tacgataaag atgtttattt tacacaacat
    14161 acttttatcc acgaatcagc ccttcgatta caaaacatca aacgagaggt tatagatatt
    14221 tctatagatc aaggaaaatc acagatcgtt catggaaaaa atatagaagc tgattatatt
    14281 gtaggttggt ttcttgaaat gagtaataaa tattatatta aaaaaatcgc tatggatatg
    14341 taccgtgcaa aaatattgaa gcccgcttta gaagaagcag gttttactgt ggaaattgtt
    14401 cgaagcggat ctgttacaca tggtatgtta aaagatctgg ttgatgacct ttttattaat
    14461 caacgtttat tttttggtga cgatgcgatt atgcgttggt attgcatgaa tgtatatgaa
    14521 gagcatattt ctaatggaaa tatacgctat gaaaaaatag aacctgaaac tagaaaaacg
    14581 gatggctttt tttcattcct tcatggtttg aattttttag atgatattta tgattctgct
    14641 cctgtaacag tcacaaatag ctcagtagaa aatacaggaa ctggatttac tcctctagta
    14701 ttctaacttg aaaggaggtg agaaagtggg gatttttcaa aaggcggtag gatacttcac
    14761 aaaaaaagca acggttcctt tagaagaata cttttgtaaa ttgcaagttg attttgtgta
    14821 tcgaaaattt gcgattgaaa cttgtattga tttgattgca aatgcgatga gtaaagcgga
    14881 attcaagtca tatgaagatg gaaaaaataa aaagaatgat ctttactata ggctgaatgt
    14941 agctcctaat aagaaaaata atgcaacaga atttagaaaa aaactgatca ggagattaat
    15001 attctacaat gaagtattga tcgtttctcc gtctaataat tctagcgaaa tatttattgc
    15061 ggatagttgg gatgtcacag aatatgcatt gaaagatgat gtgttttctc aagtgcaaat
    15121 taacaacata gtccttgata gagaatttct agaaagtgat gttatctata taaaatacgc
    15181 agatcaacaa attaggcaac tagtcgatgc gtattatcaa gcgtatggga aactcatttc
    15241 tagtgccatg aatgtttaca agcgctctaa cgctcgtaga tacgtactga aagggaattt
    15301 attccgaccg caagacaata caacacaaga tcaaatcaat aaaatgatga catcacaatt
    15361 taaggctttt atggaagctg ataatgcagg tgcggtattt caattacaaa atgagtacac
    15421 attagaagat ttcagcggaa acttccaaag caattcaaga gatataaaaa acttaataga
    15481 cgacatcttt gagatgacag cagcagcgtt tcacgttccg aaaaacctac taaagggaga
    15541 catgagtggg ttatcggatc aagtggacgc ttttttaatg ttcgaaatca taccgattgc
    15601 tgaacttatt caggatgcgt ttaacgctag tctctatgaa gtagaagaat acttgtcagg
    15661 gaattttgta cgtgtggata caactatgat caagattact agcttcaaag atttggttga
    15721 cgctattgat gtaggcatta gaaatggagt atttacaatc aacgaaggaa gagaacgcgt
    15781 tggaaatgat cgctctgata aggcgatggc agatgaaata tttataacta aaaacaatca
    15841 acaagtatcg aaaggaggtg aggcgaatga cgacaatgaa aacatttcta gcagtaaaga
    15901 atgaaggcgc agtaccgcaa atttttattc agggatttat tggttctagt tggttctttg
    15961 aagggaatac tgacaaggga atcaaaaata ttttggatag tctaggtgat caagaagaaa
    16021 ttgaagtagt aattaattca aacggtggag acgtatttca agggattgct attgggaact
    16081 tacttaagtc aaataaagca aaagttaacg ttgtgattaa cggcttagcc gctagtgctg
    16141 cttcaattat cgcaatggct ggcgatacta taaaaatcta caacaatgca caattgatga
    16201 ttcaccgcgc ttccacatac ggagaaggaa atgtcgatga cttccgtacg attgctgacc
    16261 aactggaatc aattgataaa tcggtaaagg cttcatataa aacacgattc aatggcacag
    16321 atgaagcatt gcaagaactt cttgaaaaag aatcgtttat ggatgcagaa acagctttga
    16381 gttatggatt ggtcgatgaa attatcgatg cagaaaatag ctcaggtact gaagctaaaa
    16441 aagaacaaag cgttgaagaa attttgaatg acgttaaaga aaaaagagca gaaaaaattg
    16501 ctgcatttac agcagcatta aataaaacat ttggacaagg agatgtaaaa taatgacagt
    16561 taaaaattta aaaggtgtaa cagctgcaag cgaccaattg atgaaagctt ttaaagatgg
    16621 taacgaagaa tcttttagcg cagctatggt aagcttatct aaggaaattc aggataaaat
    16681 tttagaagaa gcaacagcaa aaaatcaaga tcaattagta ttaatgaacc gtggtcagcg
    16741 tgtattaact acacaagaaa caaaattcta taacgaagta gtgaaaaacg aaggttttgc
    16801 aggggtcgaa gaattagtgc cagctactgt atttgaacgt gtatttgaag atttagaaca
    16861 atctcatcca ctattgcaaa aaattacttt tgttaacaca actggtgtaa cagaatggat
    16921 tgtgtcacgt ggagtcaatc cagcatggtg gggtaaactt tgcgaagctg ttaaaaaagt
    16981 tttagataat ggctttgatg taattaacat gaagcagttc aagctatcag gttatattcc
    17041 tgtatgtaag gcaatgcttg atttaggtcc agtatggtta gatcgttatg tccgtactgt
    17101 tttagtagaa tcattgagaa ttgcattaga acaagcaatt gttgatggta ctggtaaaga
    17161 tatgccagtc ggaatgatgc gtgacatgag caaacaaact agcggagaat atgctgaaaa
    17221 aaaagcagaa cctattacag ctttagatgc tgtaactatg ggcggtttga tggcgcgact
    17281 atcaaaattc aatatcgaag gtgtgaatga tccgatttat cgtaatgtga atccttctga
    17341 tgtggtccta attgtgaatc caactgatta ctggtctaaa gtattcccag ctaagactgt
    17401 actaactgct aatggagaat acgtacaagt attgccagta ccagtttcag atttgcagtc
    17461 aacggctgtg ccagaaggaa aagcagttat tggggtagcc tcagattact tcatgggtgt
    17521 aggatctaca ctaaaaattg aagcttcaga tgaataccat tttgttgaag acgaacgcat
    17581 ttatctagct aaacaatatg caaacggtca acctaaacgt aacgatagtt tcattgtatt
    17641 agatattagc gctttgggaa ctactactac aactacaaaa ccaacaacca caacaactac
    17701 aacacaagcg taggtgatca gaatgaagta tattctttgt cagccggcaa tcaatcggtt
    17761 taaatgggag cttgaagttt gtttaactaa tctgaagaaa ctaggaatca aagatatcgt
    17821 attgcttttc agcagacacg atgatcagat tcctattttt cttgagaagg aatatggtgt
    17881 tgaagttcat gtgtacgatg atctgcggga cgacaaagag tatattcctt cgattaaacc
    17941 atatttatgg tggaaatatt tagaagaaga tcattcgcgt gaggacgacc gatatttcta
    18001 tatcgattcg gatgtcattt tcaataaaag aattaatttg cgcaaattgc cttctaaaga
    18061 tgatgtttgg tattgtagcg actgctgtag ttatctaagt cttgattata ttagaagctg
    18121 tgaaaacgga gaaaatattc taaaagatat ggcaaacatt gtaaatgtta cagtagaatc
    18181 tttggaaact ataaacacta attcaggagg cgcacagtgg gttattaacc gtcctaaagc
    18241 gaattatcgg aaaaaggttt atctggattc taatcggcta tatcgctacc ttagagggca
    18301 aaaaacaaat atacaaatct ggacagccga gatgtgggca cagctttgga acatgatgta
    18361 tttcaatatt ggtcctaaag ttcacgagga attagacttt tgttttgcta ctgatccaat
    18421 agaaaaagtt aaagaagtaa aaatcttaca caatgctgga gtaacaacaa acgatgaaga
    18481 tttatttttc aaagggagat acgtgacttc tacgcctttt gatgaagatt tatcatttgt
    18541 aaacaagaaa aaatgctctt acgcatatgt taaagcaatt aaggcggtgg ttagatgacg
    18601 cctgaacaag tgactgaaga attgctaata gctgtgaagg ataatattta cgttacctgg
    18661 aacgaagaag atgagtcaat taaaaagatg atagctaaaa atgctgttta tcttcaaagt
    18721 aaagtgagta caacactttc tttttctcct gaaagcttag aatacggatt gctaatcgaa
    18781 agatgtagat acgactggaa tcgtgcttta gatgagtttg aacaaaattt cgctagtgag
    18841 ttattaggtt tcattcaaca ttatgcgcta caagaatata ttgcaggtga tgtgaatggc
    18901 gaataatcgt agactcgaag aaacattcaa cgatggttgg ttaaagattt tgacgcaaac
    18961 taccaaaaga aatgaactag gaaaaaagat tggtgtagaa gatacagaaa tcacttcttt
    19021 aaaatttaga aatctttcca tgagagatag tgatataaca gctatggatg cgatgggatc
    19081 gaaattaact aagaaagtaa agactccatt tcatccaatc gccaagaaat ttaataaaga
    19141 tcaatatttt atcgtaatcg atagtatgcg ttacaacgtt atctatgccg attacgataa
    19201 tttttatatc tatttttatc ttgaaagtgt gggtgaatat ggtgattgat aattctaaag
    19261 aaaaagaacg tttaaataag caaatttctg ctatcaaaac ttccttagaa gagcattttg
    19321 gcctcaaact ctttcaagac tccgtaggcg aggatgagct acctgatgat tttaattact
    19381 tcattctcga aacaggagaa atagaaatga tcactgagcc aaaatatagc gtgggtcaaa
    19441 atctatatct aactttctat tcagaaaata gagaagattt aacaggagac tcactagata
    19501 ttatttcatt gattcaaaat cgttcgattc gttttcagag aatggatccc aatcatttaa
    19561 aactagagaa ccaagatcgc tatatcgatc aattggtatt tacgtttaga cgattattga
    19621 agagtgattg tcatggctaa aaatagttgg gagctaaaaa taaatggaca tgatgaactt
    19681 cttgtgcgga tggaacgcta ttcaagcgag agcgaacgac tgattaacga agcattgaaa
    19741 tcaaaaggtt cggctattgc agtggatagg attacagaaa aaattcctgt ttctgaagca
    19801 gatttaagaa gagggcacca acacgcaaaa aatagtcgtc cacttaagac tcaatacatt
    19861 aatttgggtt tcatcattag acctacaaga aaatttgagt atttaaaata tcctgatttg
    19921 gggataggta cttctaaaag aaatcagccg gacgaattta tgagaagagg attaggtctt
    19981 gcacttgatc caattacaga acttctgatt cgtcaattcg ataaattaaa taaatagggg
    20041 gaacaacaat ggctaaaaca aaaactgtag taacaacgtt cgataacgta agtatcaaac
    20101 gaattgcttt taattttaag aacgcaggaa atgcaatcgc aacgaattgt aatggacaat
    20161 tagatggcga aacagaaatg caaacggtgg ttaaaaaatg tggagcgaca gaagtaaaat
    20221 caaaatctaa accaaacaat atgacggtaa caattactgc acatgtaccg atggaagttt
    20281 atcgacgttt caatgggttg aaacaagatg gacgtattaa accaggcatt tactcttacg
    20341 gtcctgattc cgtaggcgaa gatttctcac ttgctgcaga gatcgtggat gacttcgaag
    20401 aaaatagaaa gttagttggt atgttagcat gcacttcgaa tacaggatta acattctcta
    20461 ttgaaaatgg tgcggatgaa gtagctgcgt tagaactaga aacaaaagtt atgcaagatg
    20521 aatttggtaa attttatcat gaagcaattg ttgcagaact tgaagaagac ttaacagatc
    20581 aatggatgac gaatctatct gctgatgtga ttaaaaaaac ttcagttgtg acaactacgg
    20641 ccactcaatc acagtaaaaa aaacggaggt agcgaaatga acgaagatta ctcaaaaatt
    20701 gaactaaacg atggaacaat tttgaattta gaacctaaac tgaatatcaa gaaattattg
    20761 atgatcaata gagattttaa cacagacgag tttgcaaaaa tgtcgatggg aaaaggctct
    20821 atggatatta cagttattca aggtgcaaaa gccgtatatg tagcttatcg tcaagcgaac
    20881 atggtcgatt acatttcatt cgatgaattt atagataaat gggattttga tatggaggtt
    20941 gcagtagctg tatacagtac tatgatgttc aaacaagcac gtgatgccta tcaaaaagaa
    21001 ttcgaaaaag caaataagga aaaaaagctt caaaagtaaa aatgccaaag ctcttagttg
    21061 aaacgtgggt cgatgtctat tcgatgttga ccgacgtttt ttctatgcct tcagatttgg
    21121 ttttaagcga tatctgttta gatgacattt tgcaaatggc ttacaacaag agcgcttatg
    21181 aaggatggaa gaactatgca ataaatcaat cccagaaaaa ctaaaggaaa ggaggtaaaa
    21241 aatggctaaa aagagaacgg aagcagaagt aactttcata gctaacgatg acggattgaa
    21301 atctacgtta aaagaaatca gtgctgaatt aactaaaaat agagcagaat taaaactaga
    21361 acaagctcaa ttacaacaga ctggttctga atcagacaag ttaggaagta aattatcttc
    21421 tttagaaaag cagtatgaat tacaaagtca aaaagttgaa gtaactagtc aacgtttagc
    21481 caatgccaaa aaatattatg gagaaaattc caccgaagtt cagaaacttg aaagagaact
    21541 gattaatcaa caaacagctc agcaaaggtt atctaatgat atagataaaa cgagtaaggc
    21601 attggctcaa tctaaaggtg aatttaaaac gtatgagtca actatgaaag acttagataa
    21661 tgagcaacaa catctaaagt caagcgcatc attagtagaa tccgaatata aaaaatggca
    21721 agcaactgct ggccagtctg ctacagaatc tgaaaaatta gcaaaagcac aagaatatgt
    21781 cggcaaacag agtgatattg ctgaacaaaa aatcgaagtg ctaaaaaaac aattagatgc
    21841 cacacaaaaa gaatttggag aaacatctac cgaagcttta gaaatgaaaa ctaagcttaa
    21901 tgatgcggaa agagaattcg aagaattaag taatgctgct aaaaacgttg atacctccac
    21961 agtagatgat atcggcaaaa aacttgatat gggtaattta atggaagcta ctgatcactt
    22021 gtcagtgatc ggagataagc ttattgatgt aggtagtaag tctattgaag cagctggaaa
    22081 agcacaagct atgcaggccc aatttaaaca agtctttggc tctttagaag gggaagcaca
    22141 ggacgccgtt gagggaatgg ctgaagaatt tggaatgtta ccaaatacga tcaagcctgt
    22201 ttttacacaa tatacgtcaa tgtttaaagg acttggatat gataccaaag aagctatgga
    22261 gttagctggt gatagtactc agttagcagc agatgcagca gctttttacg ataagtctat
    22321 ggatgatgct agtgaatctc ttaattcatt tataaaaggg aactacgaag gtggggagca
    22381 aataggttta tttgctaatg atactcaaat ggcagcttat gctgttaagc ataatttgat
    22441 accagcgact gaaggagcaa aaaaagccag tgaagagtca ttgttagctg ttgaaaaagc
    22501 acaatctaag tatgctgatg ctattaagaa acatggtgaa ggatctttag aagcaagaga
    22561 ggctgcttta aaacttaaag atgcgcaaga taaaataaat gaagaattag gcccacaaac
    22621 gcaaaaatgg tctgatttag atgaggctac caagcaagca gttcgagttc aatatgctga
    22681 agatatgcaa aaattagccg gtgctacagg tcaggctagt agggagtctg atggtttaga
    22741 aaaccaaatg acaagagcaa agcaagcgtt agaagatttt tatgcctcat tgggtgaaga
    22801 tatactgcct gtgtttatca aagggttgca agcaggagcg aaagctttgc aaggattagc
    22861 cgaatggtgg agtaaacttg atgggccaat gaaaaatttc attttagctc ttggaggaat
    22921 tctagcatta ttaagcacat tagctcctgt tataaccgca gttgttacga tagttggcac
    22981 atttggttct acagttttgc tgccaataat aggaattata gcgggcgttg cagctgtaat
    23041 agcgattgta attacagcgt ttcaaaactg gggcgcaatc actgactggt ttagtgattt
    23101 atggaaaaaa tttaccgatt ggttaggtga tacctgggaa agtataaaag aaagtgcctc
    23161 atcagtttgg gatggagtca aagaaacctg gtctggattt gtagattggg ttcaagaaat
    23221 ttggcaagga gtttctgatt ggtttggaga gttatggagc ggattagttg aaggagcttc
    23281 caacatctgg caaggagtcc aagagacttg gcaagcattc gttgattggg tttcaaatat
    23341 ttggaacgga gtcaaagaag tatggtcgat tatttgggca gacattgtag gaattgttca
    23401 aataccatgg acattaataa cgtcattgat tcaagctggt attaatatta tcgtgggtat
    23461 ttttgatgta gctggacagt tattaggcgc agcttggcaa gctgtttgga cacctatttc
    23521 tgatttcctt aaaaatactt gggatactat gacacaatgg ataagcatcg cttggaatgg
    23581 aattgtaact acattccata ctatatttga tccagtagtg gcatggtgga atggtatatg
    23641 gacatctatt agtactacgg cttcaaatat ttggaactca attagtgcaa cagcttctag
    23701 tatttggaat agtatcaaga atacaatcac tagcttggta caagcagctg ctacagtaat
    23761 tcaaaatatt tggtcaactg tatctagttg gttaggtgga atttggaatt caatcagctc
    23821 tacagcatca aatatctgga gtagcgtgac tagcagtata agcaatgcta taaacgcagc
    23881 taaaagtgcc attcaaagtg tttggaatag tatatcttcg tggatcagcg gaatttggaa
    23941 cggtatcaaa aacactgctt tgaatctttg gaatggaatt acaagcacta ttagctctaa
    24001 agtaaacgat ggaaaaaatg caatttcaag cggttggtcc aatctaacag gtattgtttc
    24061 cgacatattc aataatgtta aaagcacgat agctaatata tgggaaggta tcaaaaagac
    24121 tgttagcgct ccgattgatt ggattagaga taaaatcagt agtgtctttg ataatttgaa
    24181 tatttcgata ccacatattc cgttaccaca ttttaaattg agcggagaat tcaatccatt
    24241 gaaggggaaa atcccaacat tgggtgttga ttggtatgcg aaaggtagtg tgtttaattc
    24301 tccaaatatt atcggtgtcg gtgaagcagg acctgaagca gttttacctt tgaaaagatc
    24361 tgtgctgcaa gaaattgggg atcgtatctt gagtagcaca tcagtttcat ctagggcaca
    24421 aacgattcaa cctgtgaata actacgaatt caatttcaca attgatggta acgcagatga
    24481 ggttactatg aagcaaacaa ctcaacaaat cattgatagc attacaaaag ttcaaaatga
    24541 taatgcttcg gcatggcgtt aaacaggaga gtatttctcc tgttttttta gttttaaaaa
    24601 ggatgtgaaa aaatgactga ttgtatacat tctataatcg atggatttcc tgattatttg
    24661 cataaattgg ctttagcgga aagaccaacc ataccttctc caaaaagaca gagagttgaa
    24721 acttctgttt taggacggct aggtggctta gtacaagatt actcgtttga agatatgtcg
    24781 tttacattgc actataacta tttagaggat gtggaagacc atcaagcgtt caagcaatcg
    24841 ttttatatca tgcgtcattg gttaaactat gcaaagaaat tagaattctc tgacgatccc
    24901 aacgtctatt acgttatcca gactatcgat attggggatg cagaaaacga tattgttgaa
    24961 tggggagagt tcgatgtaaa tattactgcg aaaccattcg caagagttca agaagatgta
    25021 cctataaccg tagataaacc acagtcattt aacttgctga ataatagttt agaagaaagt
    25081 tttccaaaga ttatcatcac tccttcagct acttcatgcc agttcatctt aaatgattat
    25141 gtgtttagtt tcgaaggctt agtagcagga actgatgtag tcattgatag tgatttgatg
    25201 ctttgctacg aagagcaatc ggacggagat attttagatc ggtccaacaa aatgaagacc
    25261 atgcaatatc cgacattgca agtggatatc aatcatttta attgcactgg tttgagcaaa
    25321 atacaaattt atcgtaacgg gttaaggtag gtgaaataga tgatcgataa tttaataact
    25381 atttacgata aaaatgacgc gaataattta gctgaacatt tatatgatac gcaaggttta
    25441 ggcgctttgt cagactggtt aacagctact gttagcaata aactaaacgg agccgagata
    25501 tttcagggta cttattcaat aaacggaact aatgcagatt tgattgtaga aggacgtatt
    25561 attcagtgtt atgtagatga aaatcgagca aaacagcgtc tacggattta ttatgcaaaa
    25621 acttctgtaa taggcaatac gatagaagta aaagctgaac ctattttcaa tgatataaga
    25681 aaatcggtgt tgaataaata tgacagcgga acagaaaaga tcactgctac tcaggcatgg
    25741 caaaacgcaa aagttttagc gaaaccagct atcccttcgc agttttcttt ctcgtcatta
    25801 gtagatacgc ttgctaatgt gaagatagaa aaggcgaatt ttttagaatt ctttggtgga
    25861 aaagagggat ctattctaga tcgatttcat ggtgagtttc taaaagataa taacacatta
    25921 cgtcatgaaa aaagtctagg tacggatcat aaaatcaaag cgatttatac taaaaactta
    25981 actggtcttg acttagagat tgatgctcaa agtgttttag ttggagttta tccattcatt
    26041 agcagctctt cagaaggaga agacgagatc actctaccag aagaagttat tttcacggat
    26101 tacgtggatg attatcctgc tggatatgtt tcttttgttg attttaaaga caaagcgact
    26161 gatgtagcca cattaaggga agctgctaaa gactggttga aaacaaacat agataaacaa
    26221 aaaccacaag tgagtggttc gattgaatta gtaccattga ggcatcaaag aggctatgaa
    26281 aaatttgttg atctagaaaa agtttcgatg ggtgacggag tagatgtgta tcatccacag
    26341 ttaaaagtga atatgtcagc gagaattgtg gaatatacgt ttaatgttct aaccaattca
    26401 tacgataaat tagttgtagg aaacgtcaaa acaaacttct tagaaaacac agagaataat
    26461 gtcagcaatt tgattaatga tgccattgat caattgaaaa acggtggcga aatcagtgat
    26521 ttactcaatg atattgtaga tcatcaaact gatatgatta ctggtcaaaa tggtggttat
    26581 gttttattag atcctaaaga agcgcctagt cgtattttga ttatggacac accagataag
    26641 aataccgcaa ggaatgtttt acaaatcaac aatgctggta ttggtttttc taaaactggc
    26701 attaatggaa catatgaaac ggcatggacg ttagatggcg gattcaatgc ctcgtttatt
    26761 acggctggtg aaatagtagg aattactatt agaggtacta cattaattag tgatggtgct
    26821 gattatagaa caagtattgc taatggcaaa atgacttggt actcaaaaaa agttaacaaa
    26881 gatattatgg agctagaagc acgtgattat gtaagtgctg atgccggtat tgtatcatac
    26941 accatgaaaa ctggtggtgg tttcatgatt agaaatccac agggtaactt ggtttttagt
    27001 acgtgggata atggtaataa cagaccgttt ttatcttttg gtgcgccaaa tttcagatat
    27061 agcaatgcta gttatgtaac ttctggcgat ggtaattctt tgggtataga tggtagtgcg
    27121 ggtaactcat gggaatttaa ggtagctggt aggactatga aatttactag tgatggtatg
    27181 ctaacgttac caggttgttt ttttggttca tgggaagatg ggaaacttgc taggtttgaa
    27241 caatcaacgg tacaagtata ta
//
LOCUS       DAXWOO010000020        27253 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.020, whole genome shotgun sequence.
ACCESSION   DAXWOO010000020 DAXWOO010000000
VERSION     DAXWOO010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.020"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(176..1462)
                     /locus_tag="ACGE70_000698"
     CDS             complement(176..1462)
                     /locus_tag="ACGE70_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFF8692354.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(1455..2723)
                     /locus_tag="ACGE70_000699"
     CDS             complement(1455..2723)
                     /locus_tag="ACGE70_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFF8692355.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            2975..3436
                     /gene="mscL"
                     /locus_tag="ACGE70_000700"
     CDS             2975..3436
                     /gene="mscL"
                     /locus_tag="ACGE70_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFF8692356.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(3495..3941)
                     /locus_tag="ACGE70_000701"
     CDS             complement(3495..3941)
                     /locus_tag="ACGE70_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8692357.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(4034..4279)
                     /locus_tag="ACGE70_000702"
     CDS             complement(4034..4279)
                     /locus_tag="ACGE70_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFF8692358.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(4394..4972)
                     /locus_tag="ACGE70_000703"
     CDS             complement(4394..4972)
                     /locus_tag="ACGE70_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFF8692359.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(5049..5579)
                     /locus_tag="ACGE70_000704"
     CDS             complement(5049..5579)
                     /locus_tag="ACGE70_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFF8692360.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(5583..6443)
                     /gene="rlmB"
                     /locus_tag="ACGE70_000705"
     CDS             complement(5583..6443)
                     /gene="rlmB"
                     /locus_tag="ACGE70_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFF8692361.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(6436..6831)
                     /locus_tag="ACGE70_000706"
     CDS             complement(6436..6831)
                     /locus_tag="ACGE70_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFF8692362.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(6828..8240)
                     /gene="cysS"
                     /locus_tag="ACGE70_000707"
     CDS             complement(6828..8240)
                     /gene="cysS"
                     /locus_tag="ACGE70_000707"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFF8692363.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(8237..8773)
                     /gene="epsC"
                     /locus_tag="ACGE70_000708"
     CDS             complement(8237..8773)
                     /gene="epsC"
                     /locus_tag="ACGE70_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFF8692364.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(8859..9098)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(9095..10555)
                     /gene="gltX"
                     /locus_tag="ACGE70_000709"
     CDS             complement(9095..10555)
                     /gene="gltX"
                     /locus_tag="ACGE70_000709"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFF8692365.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(10725..11882)
                     /locus_tag="ACGE70_000710"
     CDS             complement(10725..11882)
                     /locus_tag="ACGE70_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFF8692366.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(11916..13286)
                     /gene="radA"
                     /locus_tag="ACGE70_000711"
     CDS             complement(11916..13286)
                     /gene="radA"
                     /locus_tag="ACGE70_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFF8692367.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(13349..13876)
                     /locus_tag="ACGE70_000712"
     CDS             complement(13349..13876)
                     /locus_tag="ACGE70_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFF8692368.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            14106..14912
                     /gene="proB"
                     /locus_tag="ACGE70_000713"
     CDS             14106..14912
                     /gene="proB"
                     /locus_tag="ACGE70_000713"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFF8692369.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            14909..16156
                     /locus_tag="ACGE70_000714"
     CDS             14909..16156
                     /locus_tag="ACGE70_000714"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFF8692370.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            16232..16576
                     /locus_tag="ACGE70_000715"
     CDS             16232..16576
                     /locus_tag="ACGE70_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692371.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(16693..17019)
                     /locus_tag="ACGE70_000716"
     CDS             complement(16693..17019)
                     /locus_tag="ACGE70_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692372.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(17392..18593)
                     /locus_tag="ACGE70_000717"
                     /pseudo
     CDS             complement(17392..18593)
                     /locus_tag="ACGE70_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            18710..19225
                     /locus_tag="ACGE70_000718"
     CDS             18710..19225
                     /locus_tag="ACGE70_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFF8692373.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(19424..19870)
                     /locus_tag="ACGE70_000719"
     CDS             complement(19424..19870)
                     /locus_tag="ACGE70_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFF8692374.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(19917..21395)
                     /gene="msr(C)"
                     /locus_tag="ACGE70_000720"
     CDS             complement(19917..21395)
                     /gene="msr(C)"
                     /locus_tag="ACGE70_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFF8692375.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(21749..22378)
                     /locus_tag="ACGE70_000721"
     CDS             complement(21749..22378)
                     /locus_tag="ACGE70_000721"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFF8692376.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(22362..22826)
                     /locus_tag="ACGE70_000722"
     CDS             complement(22362..22826)
                     /locus_tag="ACGE70_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFF8692377.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(23313..24581)
                     /locus_tag="ACGE70_000723"
     CDS             complement(23313..24581)
                     /locus_tag="ACGE70_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFF8692378.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(24812..25549)
                     /locus_tag="ACGE70_000724"
     CDS             complement(24812..25549)
                     /locus_tag="ACGE70_000724"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFF8692379.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(25616..26185)
                     /locus_tag="ACGE70_000725"
     CDS             complement(25616..26185)
                     /locus_tag="ACGE70_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFF8692380.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            26313..27185
                     /locus_tag="ACGE70_000726"
     CDS             26313..27185
                     /locus_tag="ACGE70_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFF8692381.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
BASE COUNT         7675 a         5784 c         4918 g         8876 t
ORIGIN      
        1 gattacttcg tttgatttta ttttaatata agttgattaa aagagagcat gatttgtttt
       61 attttctcac taatttttcc aacacaacta agaataccat aaaagctgat tttggaggag
      121 gttcctacta caagtctaaa acgaaattta agacttttcc tcataaaata ctggtttatt
      181 ttggtctcat gtaaaactcg ctgaaggcct cttcacttat aaatgcttcc ccagctgcca
      241 gtacgtcttt catttggatc gaatcgatga tttcaggaag atcaaataaa gttcgatcgc
      301 caaataaact ttgcgtaaat tgattggcaa tatattcgat cgaatttaat gattggaaat
      361 attgtccaag cattttcttc ttcagcaaat cgagatttgt ttctgacact tccggatcat
      421 cagcgaatcc aagaatgatt tctttgactt tttctgctgc tttctctggt tcgtccgtat
      481 ctccgctgaa gtccgcaaag tgaaattcac gatccaagct gaattcaaaa ccaaagctgt
      541 catcgataat tccttgattg tacatcgcta gatagtttcg agaagtattg cctaaaatca
      601 tttggaacaa cagattgatt gctgttttat agcgtaacag ttctgttcct tcttgcggca
      661 atgtgtctaa tcctttaata ccaagtacaa atttatctcg tgtaatggct gcttccaatg
      721 cactttgttt gatgatttct tttgtgtttt cagggaaata acgtacaatt tcttgctttg
      781 gagggaaatt ttttgcttct tggttttccc gaataagttt catcaatttc tcaggctcca
      841 tcttaccaac tacaaataaa accatattgc ttggctgata aaacgtccga tagcacgtat
      901 acaagtcttg agctgtgatt ttgtcgatac tttcaaccgt gcccgcaata tcgatatgca
      961 atgggtgtgt aggatacaag ttatttagta tgccaaaaaa catccgccag ttagggtcgt
     1021 cttcatacat ttggatttcc tgaccgatga ttcctttttc cttgtttaca gtttcttctg
     1081 tgaagtaagg tgcttgtaca aaatcgatca gagtcgtcag attcttttct acctgatccg
     1141 tcgtagaaaa cagatagctg gttttcgtaa aacttgtgaa agcattggcg gaagctcctt
     1201 gtttcccaaa taattgaaag acatcaccat cttctttttc aaacaactta tgttctaaaa
     1261 aatgggcaat tccatctgga actttcacct tttctttttc accataggga ataaattcgt
     1321 tatcgatcga accataattc gtactgaaca aaccatatgt cttgtgatag tcatttttag
     1381 gcaacaaata cacggtcaaa ccgtttggca gtacttcatg ataaagtgtt tcattgattt
     1441 gctcatattc tgttttattc atttgcattt cctcctgcta aaaagaagat tgcctgcaat
     1501 tcgatttgct cagctacttg ttgtatctct ttgatcgtta cgctctccat acgtttcaac
     1561 cattcttcat cagataattt tgtttccggc agccagctgt ccaaaaaaga agcttcgatt
     1621 gccgcttgcg gagagtctag agaaagcaga tattggtttc tcagcattgc ttttgtctga
     1681 gacagttcca actctgttat ttcaccattt cgcaaacttt ctaattgctc gtgaatcaga
     1741 tgaagtactt gatttcggtt cttttcatcg ataccggttt ggacactcat gaatcctcgg
     1801 aacgtatcca cgctactaga ggcataataa gctagacttt ctttctctct gacattcatg
     1861 aaaagtttag agtgaggaaa accgccaaat aaaccgttaa agaccattag cgcaaaacgt
     1921 tctggttcat cgtaatacac atttgtctga taagctaggt tcagctttgc ttgaacgatt
     1981 ggctcacgta cttgtcgttc cttcacgata ttcacttgag gttgggtata aaacagatct
     2041 ggataaactg tttcccgcga ctcaaatggt aattgcccaa ctgcttcagc tgctttgtcc
     2101 ggatcaacat cgccgacaac aaaaatgtcg atttgatcat ttgccatcat ttcttgatag
     2161 taggacgcta gcgaactagc agtgatcttg tccaaatctt cgactacgcc caaccctggg
     2221 atcttttgtt ccgggctgtt ttcaaaataa agttcttgca aggcatagga agcaaaagtt
     2281 tgtttatctt ccttcaaact ttccaaatat agacgcagat tgttcttttc caagtcaaac
     2341 gtcaactgat caaactgatg atttttaata tttggataaa ataaaacctc ttttaaaaaa
     2401 tctaccgctt gagaaaaaag gtttggatca ttgacatatt caccattcac aaaactgatc
     2461 cctgcgttta gccaatgtaa atttcctttt tttctaactg ataatccaaa gcttgcgcca
     2521 taaagctctg caagttgcgc acttaacttt gtctgatccg gataattcaa actatttgtt
     2581 tccaacatgc tgctgagaag tgaacgtttc gtgatgactt cttgttgcag acgcgcagta
     2641 aaacggataa aaatccggat cgttttgtat tgttttgttg gtaagacatg aagattcacg
     2701 ccttctgcca attgatacac catatttata tgctcctttt cacagttgat tgcccgctta
     2761 ctcttttaat gggtcaacct gcctttttta cttattcctc tgcttgaaat ttataagttc
     2821 aggcaattcg ccgttttttc taaatgatgt tacactttat agtatagcat agatgttttt
     2881 tatcaaaaga aacagacggc taaaagcgta tattgttttt ttatttttta tttttcgata
     2941 tactaaagac aaataaaaga agggagggtc attcatgatc aaagaattca aagagttcat
     3001 catgcgtgga gatgtcttgg atcttgctgt cggcgttgtt attggtagtg cgtttacaag
     3061 catcgtaaac caagtggtgc aaggcttgat cacaccacta ataggttggt ttgtcgcaat
     3121 gatcacaggt acttctgatt tagaaggagc actttcaatt cttgactggt cgccgacaaa
     3181 aggcgtcaca tttgcgtttg gtaacgttat tagtgctatc attacatttc tgatcactgg
     3241 ttttgtctta ttcttagttg tcaaagcggc aaaccgtgcg aagaatttac gctccaaaga
     3301 agagcaagtg gaagtggaag aaactcctac tgcagaagat tatcttagtg acatacgcga
     3361 tcttcttgcc aaacaagtgg cagaagacaa tacacatcca gtagagcaaa caaatctttc
     3421 cgatacttct aaataataaa gaatctaaaa aaaagaaagc cgattttcag tgtcggcttt
     3481 ctttttttag attcttaact tgctacttgt tcctttttca tgtcgatatg tggaatatca
     3541 tcttccaaat acacatctga gattgccttg aatccaaacg actcataaaa agcttgtagg
     3601 tattcttgtg cgccgatcac gattggtttt tctggaaaat ttttctggat catgttaatc
     3661 gtttcttgaa caatctttct tcctaaacca tctttacggt tgtcctgatg aacaaggaca
     3721 cgtccaaaat ggatactctc cgcttcttca tatactcgcg tgtaggcaag gatcttccca
     3781 tcagtatctt gaaaaaacgt atgccaagct tgtccatcaa tgtcatcaac ttcttgataa
     3841 ggacactgtt gttccacaac aaaaactgcg attcgttctt tgacgatcgc gtaaaactct
     3901 gctgttgaca attcttcaaa tttctttacc tgatattcca ctaagcaaac gccccctgct
     3961 tttttattct tagcataccg attcgcacac aaaaaagcaa acgaacctta cgaatctcag
     4021 gctcgtttgc tttttaaact gcttcagtta aaaattcgat ctcaactgtc cgagtaagta
     4081 catctgaata gctgtaagat actcgttcaa aagaattttc ttcaggatcc aaatcaacaa
     4141 cgaatactga aggataggtt tctgttaaaa cgccgcggcg ttctgtttga cgctttcttc
     4201 ctgtctgagc aaccaacatg attgggctgc cgatacgacc ttctaaatct ttcttgattg
     4261 aagctaaagt tgttggcata cagttcacct ctactatcaa ctataccaca tatttttaaa
     4321 attttcaagt ttaccataaa aaaaaaagaa atgcaataaa aataaataag aagctcatta
     4381 agatttttct aaattattcc tcattataaa atttcaagtg tttttttatt ttgagtttta
     4441 ctctcccgta agcgctcatt acttgcttgt gatccattgc taaacattca gccatgtcta
     4501 caaaatcaac gccttgcata taataatgaa aaattttagc ttctattttt gaaaactgga
     4561 tttcgcaatt ttcgattgct tcttttaata aaagatgctg aatcgctaaa ggttcttgca
     4621 atgtgatctc tgctaaaaag tctggtcctt ctgtctctat tttttggtcg attgagactt
     4681 catctttttg atcacgacgc ttcagcgcgt tctgcttcct caacagagaa tagatttgat
     4741 tgcttataat gcttttataa taacttccaa agctaacacc tttgcttttt tcgtaagttc
     4801 tcaatgcctg atataaagcg atccttgctt cttgttccca atcctcagaa acataatttc
     4861 ttaaataata ggtgtttttc aaacgtagta aaagtggaaa atatttttga tacagggcag
     4921 caaatgcttc ttcgtcacca ttacgtgcag cgcgcaataa ctcctcagac ataaaataat
     4981 tcctccataa attcgtttat ggaggaatct tatcatcaat gattcatcga actgattaga
     5041 acgatatgtt atttattttt tctcattaaa tcgtccaatt tttgggacaa tttttttaac
     5101 tgctcttcat tccatggaga attacgtcgg aaatcaataa aatgcatatc tttattttct
     5161 tgggctattg ttttttccgt tttttttatc gtcttatata attcataagc agaagttcgt
     5221 aatgctcctt gggaaaagat catccactgt tctgctaaat cgcttgtcgc cactgtcact
     5281 tgtgtcaaac ggtcattcag ctgaccagcg attctctcga tataactgtc agccgtttcg
     5341 tcttctttcg taaaaacaac atccaactgg tatttcttat aattttgttg gatgcccgga
     5401 actagttggg catcgaagac aacgatgact tccattcctt catatttcgc ataatttgat
     5461 aaacgatgta aaagagcttc tcttgcatcc gccattttgt cctgcttttt aagctggaca
     5521 agttccggcc acgaaccaat catgttgtag ccgtctacaa tcaacagctg ttttttcatg
     5581 gtctactccc cctgacgttg ggaaaaagcc tgatacataa gcaaaccagc agcaacactg
     5641 gcatttaagc tctggacgtg cccggtcatc gggatcgtca gcaattcatc catttctttt
     5701 ttcaatcctg gactcattcc tttgccttca ttaccaatga ctaaagcaat tgatcctttg
     5761 gcattccatt tttggaagtt tgtgccggtc atatctgtgc caaataccca aaatcctgtt
     5821 tctttgagct gttgcacagc ttgtaccaaa ttcgtcacgc gtgcaacagg gacatgttcg
     5881 actgccccag tggaagtctt tgttacgata ggtgtgattc caactgctcg gtgttttggt
     5941 atgatcacac catcgacgcc acttgcgtct gctgtccgta aaatcgaccc aaaattgtgt
     6001 ggatcttcga tgctatccaa aatcaaaaag aaggggtctt cttttttctc ttttgttcgt
     6061 tcaatcagtt ggtcaaggga taaatattca taaggtgtaa tcgcaagaac gactccttga
     6121 tgcacgccgt gatcgctcaa agtttctaat ttttgtttag gaacccattt gactggcaca
     6181 gcttgttcac gtgcgatttc tttcagttct tctacttttt ctcctcgtga atcttcttga
     6241 agaaagagtt tattccctcg cccttgttgt agcgcttcta ctgcggcatg gtgcccaaag
     6301 acgaaattat cttctaatgc ttcttcattt tgatcagaaa ctgactgatt tcgtccagtt
     6361 tgctttttat ttgaatatct attttgtttt ttgtcttcaa atccacgttt cgattgatgt
     6421 ttgtttgttc tttctttacc catttttttc accaactttc tgaatacacc agcggatcaa
     6481 ttcttccatc cgctcggttt gttttgtcaa atgcagatag cccatgagcg cttcgaaccc
     6541 tgttgcgacc cgatacgtcg taacatcagc attttttgcg ctcgtatggc ttttagcatt
     6601 tcttcctctg cgataataaa gttcttcttc ttctgtcaaa agatgttctt ccagcatttg
     6661 ctgaatcaaa aaggcttgtg cattcgcaga aacataattc gttgccgcac gatgcagctt
     6721 attgggtttt gtttctcctt gatcaatcag gtggtcacga acatagattt cgtaaatcgc
     6781 atctccaaca taagccaagg ctaaaccgtt caattgtgtg taatctctca tgcttctctt
     6841 ctccatcggg ttccttgcgg tgtatcttct aataagatcc ctttttcttt caacaaatca
     6901 cgaatttcgt cgcttcggac aaaatcacga ttgcttcttg cttgatttcg ttcttcgatc
     6961 aattgttcaa tctcttcatc taataactct tctgctgtaa ataaaatacc gaagatcgaa
     7021 agccattgcg agaataaagc ttctccttgt gccaagacag cggcagaaac tttcggctgg
     7081 tctagataag tgttcagcca cttagcaagc tcatagacca ctgtgatccc atttgctgca
     7141 ttaaaatcat cgtccatttc ttcgatgaat cgctgttcta aattttctaa caaagcgatt
     7201 ttatccgcat catctgccaa attctctgag gcagtttctt ggcggaattt caaattgtca
     7261 aacgtattct tcagtcgttg taaattggtg gctgcttctt tcatcgtcgt ttcactgtag
     7321 cggatcggac gacggtattg tgtggtcgcc atgaagaaac gcaagacttg tggatcaacg
     7381 tttttgatca tttcatgtac agtgatgaaa ttccctagag atttactcat tttttcatcg
     7441 tcttcgccaa tcgttacata gccattgtgc atccagtaat tagcgaattt tttccctgtt
     7501 ttcgcttcac tttgtgcgat ttcattttca tgatgaggaa attctaaatc ttgcccgcca
     7561 ccatggatat cgatcgtttc tcctaaatgt tttgttgcca taacggaaca ttcaatatgc
     7621 caaccgggac gtcctttgcc ccatggtgaa tcccaagaaa tctcgccagg ttttgcttgt
     7681 ttccataaag cgaaatctaa tgggtcttct ttttgctgct gttcttcccc tgtacgctga
     7741 cttgctccga cttccagttc atcgatggat tggtcactta atttcccata attttcaaat
     7801 ttacgggtac ggtaatacac atcacctgaa gattcataag catagccttt ttcaaccaac
     7861 acttgaataa atgctaaaat atctggcata tgatccatga ctcttggatg cagtgtagca
     7921 ggctcgacat tcaacgcttt cgtatcttct tcaaatgctc ggatgaaacg atcagcaact
     7981 tctggtgctg taatacctaa ttcgtttgct gtacggatga ttttatcgtc tacatccgtg
     8041 aaattcgata cgaaatcgac tttatagcct cgatattcaa aatagcgtcg aattgtatcg
     8101 aaagcaatgg tgctacgagc atttccgata tggatataat tatacacagt cggtccgcag
     8161 acatacatac cgacatgtcc cttttgtata gggatgaatt cttctttttt tctggtcaat
     8221 gtattgtaga tttttatcat tctgatctcc tcactttttc tcctttgatt cgaacgactt
     8281 ttgctggtac tcctaccgct gtagcgtcgt ctggaacatc tttcaacaca acggcacctg
     8341 caccgatttt ggcgtttttt ccgatcgtta ccggtcctaa tatctgcgca ttcgcagaga
     8401 gcatggctcc ttgtttaaca gttgggtgtc ttttacccgt ttcttttcct gtgccgccta
     8461 atgttacgcc atggaataaa atcacatcgt cctcgatttc tgcggtttcg ccaatgacga
     8521 ttcccattcc atggtcgatg aacacgccag ttccaatcgt tgctcctgga tggatctcaa
     8581 caccagttag gaaacgccag aattgagcat tgatcttcgc caataaaaag agccggtgct
     8641 gatataaaaa atgagagaaa cgatgccaaa acaaggcatg aaaaccaggg tatgtcaaaa
     8701 cgatctcagc tgtcgaacgt gcagccggat cgtttctctt tgctgcatca atcgctcggg
     8761 taaaccaact catgactttc cactcctttc gtaagaattt tatgtaggaa atcatcttct
     8821 tataaagaaa agaagcgcct tttaaataag tttccttatc aaaagacgct ttccgcgtgg
     8881 ttccactttt cttcataatc aatgattatc tctgacagct aacgcctgtt atacgttctt
     8941 ggctactttg tttcctataa aacagttttt cctgttttct gtgtttcgcc aagaccactc
     9001 ccagaggcat ttcggcaacc attcattgat accttgcacc acccggtatc tctcttgtaa
     9061 tgatcagctg cttacttttt tctgttcatc gtgtttagtt cagtacttga tttaaatgtg
     9121 ctaatgattt ttcacgacca agcaattcga tcgtttcagg caattctgga ccatgcattt
     9181 gtcctgatac agctacacgg attggcatga aaagattttt tccttttacg ccagtttctt
     9241 tttgaacagc tttgatggca gctttgacag ttggagcatc tactacttcc atttgttcta
     9301 gctgtgcttt gaatgcgtgg agaacagtag gaactgtttc accagcaagt acttcttttg
     9361 ctgcttcatt caatacagga tgttcattga aaaatagcgt agacagatca acgatttctg
     9421 cagcatagct catttgaggt tgatacaggc taacgatctt cttcacccat tcgattgtct
     9481 tttcatctgg gttttctgct acacgtccat cagcgattag ataaggcaga cacatgtcag
     9541 taaatgcatc tagatccatt gctttgatgt aatggttgtt tacccattgc agttttttcg
     9601 catcgaaagc tgcgggtgat ttacttaggc gttttggatc gaacattttg atcaattctt
     9661 ggtgttcgaa gatttcgtct tcgcccacag gtgaccagcc caataatgca ataaagttga
     9721 acattgcatc tggtagatag cctaattcgc gatattgttc gataaattgc aagattgatt
     9781 cgtcacgttt gcttagtttt ttacctgttt cactgttgat gatcaatgtc atatgtccga
     9841 aggttggtgg tgtccagcca aatgcttcat agatcatcag ttgttttggt gtattggcaa
     9901 tatggtcatc tccacgtaaa acgtgagtga ttttcatcaa atgatcatcg accgcaacag
     9961 caaaattata agttggcata ccatcgcgtt tttgtatgac aaagtcgcca ccgatattat
    10021 cggattcgaa cgaaatagcg cctttcacca tatctgtaaa tgcgtattct gtattgcgag
    10081 gcacacggaa acgtacaaca gattctagac cttgtgcttc tttttctgct tgttcttcag
    10141 gtgtcaagtt cgcacatgtt cctgcgtaat gaggcatttc tcctcttgcg cgttgtgctt
    10201 cccgttctgc ttctaattct tcttctgtgc aatagcattt gtatgctcga ttgctagcaa
    10261 gcagctgatc aatcagcggt tgatagattt ctttacgttc agattgacgg taagggccgt
    10321 attctccagg attttcagga gattcatccc aattcattcc caaccaagca aggttttcca
    10381 actggctttt ttcgccatct tcgatattac gtttttggtc agtatcctca atacgaataa
    10441 taaagtctcc atcattgtgg cgggcaaaca aataattgaa taatgcagtt cttgcatttc
    10501 caatatgcag gtgcccagtt ggacttggtg catagcgtac gcgtactttc gtcattttct
    10561 tcctcttctt tcaaactaga ctttgactaa cagcaaaaat tccgtcagtt acggcttctc
    10621 aaatatcatt tatttaaagc cactcagcac aaattttacc ttttctaagg taaatagtaa
    10681 agccaactgg ttgaatttcc ttaccagttg gctgaatttc ttttttattt tgattgttta
    10741 gtctttttct ctgtctcttt tttattgtcg attcctcgtc ctgcatgtgc aggtttagca
    10801 aagatcatgc gtccagcagc tgtttgaagg gcacttgtta caacgacttc gatacgttca
    10861 ttcatgtaat gctgtccatc ttctacgaca accatcgtgc cgtcatcaag gtaggctact
    10921 ccttgttggc gttctgtccc ggcttttact actagaacat tcatcgtttc accagggatg
    10981 acgactggtt ttacggcatt tgccaacgca ttgatattaa gaacaggtac attttggaat
    11041 tcagagactt tattcaagtt atagtcattg gttacaatga caccatctaa aagcttagcc
    11101 aatttgatca atttgctgtc tacttcactg atatcttcaa agtctccatc gtacatttcg
    11161 acagatatac cctcttcttt ttgcaaggca tttaggatat ctaatccgcg acgaccacgc
    11221 acacgtttca aactatcccc tgagtccgcg atgtattgta gttcgtaaag aacaaaatta
    11281 gggatcatca aggttccttc taagaatcca gtctttgtaa tatcataaat ccgaccgtca
    11341 atgatcacgc ttgtatccaa gattttatat ttatggaaat tttctcctga ttttcgttct
    11401 aacagttgac tctcaacttg actagctgtt tcttgctcga tttttttgct tcgtgaacca
    11461 aaaactttct tccattcttc gattcgtgtc gttcccattc tcaatcccag atagcctaag
    11521 atgatcatca gtaaaatcgg caatacacta ttgacgaatg gaatttccat attgtaaaga
    11581 ggggtcgaga tcacaacacc taaagtcaat ccgatgatcg caccgatcgc accgaagaga
    11641 agataagtca agctgacctc acttagtttt tgttctactt gtttgataat agctgagata
    11701 tgtttggcca acagcaaaga taataaaaag aaaataagtg cgcctaacag gctgttggta
    11761 aatgtattat tcagccatcc gtttgactgt tgaccgattg cctgccaagc tgagggtaaa
    11821 aatgaaatac ccaagcttgc gccgacaatg atcatcagca aagtaatgac acgtttttgc
    11881 atcgtttttc ctccgtttcg acaatcattt attagttagc ggaacacacg cttcaacgtt
    11941 tcagccaaag ttgctactcc aactatctcg attccttttg gcgcttccca gttacctaag
    12001 ttgtttttag gtaggtatac ttttgtgaag ccgagttttt ggacttcttt gactcgttgt
    12061 tcaatatgac tgacacgtct gatttctcca gtcagtccaa tttcaccgat gaaacattcc
    12121 gttggctgtg ttcctttttc tttatagctt gaagcaatac tgacagcgat agctaaatca
    12181 atcgctggtt catttaattt taccccacct gcagctttta agtaagcatc ttgattctga
    12241 agaagcaacc cagctctttt ttccagcact gccatgatca gagatacccg attgaaatca
    12301 agacctgttg ttgttcgttt ggcatttccg aacatagtag gtgtgactaa tgcttggatc
    12361 tctaccaaaa ttggacgtgt cccttccatt gccacaacga tagcagagcc agttgcaccg
    12421 tctaggcgtt cttctaagaa aatctgtgaa ggattagcga cttcttccaa tccatgctca
    12481 tgcatttcaa aaatcccaat ctcattcgtt gagccaaatc ggttttttac tgctcgcaaa
    12541 atccgaaaac tatgatgttg ttcaccttca aaatataaca ccgtatccac catatgttct
    12601 aacattcttg ggccggcgat cgagccttct ttggtaacat gaccgacgat aaaaatggct
    12661 atcccatttg ttttagcgat tttgagtagt tctgcagtgg tttctcttac ttggctgaca
    12721 cttcctgcca cacttgtgat gtctggctgc gtcatcgttt ggatggaatc aatgataaca
    12781 taatcagggg agatatgttc gatggcacga ctgatttcgt tcatatctgt ttcagcgtat
    12841 agataaaatt ctgtgttgat acttccgagg cgttccgcgc gtaattttat ctgctctgcg
    12901 ctttcttcgc cggatacata taagactttt ccgcctatgg ctgctaattg ctgagaaact
    12961 tgtaacagca aagtggattt cccgatacct ggatcgccac caatcaagac catagaccct
    13021 ggcaccacac ctccgcctaa cacacggttc agttcttcta attttgtttt gatccgaggt
    13081 tcttttttag ggacgacgtc tgcgattttt gttggttttg ctttttctcc tgtcaaactt
    13141 gtccgagttc tgcggtccgt tgtatcttgt tcgatttctt cgaccatcgt attccatttt
    13201 ccgcaatttg ggcagcgtcc caagaactta ggcgacacat acccgcaatt ttggcaaaca
    13261 aattgtactt ttgatttttt tgccattgta ccgcctcctt ttatcctaat catgagacat
    13321 taaatcaagc ttaagtatct ttaacgtttt atttactgga agaaccaaat ccgccttgac
    13381 gttgagcagt cgtttgatct tgatctgctt ttaaaaacgg caaaaagatc ccttggccga
    13441 tacgctcgcc ttttttgatc actacatcct tgaaaccaaa gttggtgaat tggaacatga
    13501 tatggccttc atttgcctca ttattgtaat aatcactgtc aatcacacct actccgtttg
    13561 tcaacactaa aaaccgcttc aacggattgc ttgaacggtt agcaagctgc aaaaattcat
    13621 cttctcccat gtaagctttg attccagtag gcacgagggt tggtttcaat tgtttttcat
    13681 tttctgatac atcttttttc aatccatctg cgactgtttc caaccccttt ttccagcctg
    13741 ctttccagat acttggaatc acgacctctt ctgctgcttc aaaatcatag ccagcagcat
    13801 gatgtgtagc gcgctgcggt agattgatgt tttgatcttt atatttagaa ataacttcaa
    13861 atcctctttt tctcaccggt tttcctccct aaataaacta ctcctattct aacatatccg
    13921 aggctgtaaa agactttctt tacaaattct tcaagaaatt ctgctatctt aaagtaaacc
    13981 attaattcgt tctttttcca aacgactgca agttgtacaa aaaaactatt ttttacatga
    14041 ctttactcgt caaaacaagt agaatagaac gaaattaatg agtggatatt tgattgaggt
    14101 gtcacatgcg taaagaaatt aaaaatgcaa aacgaattat tgtcaaagta ggaacaagta
    14161 cattgattta tcccaatgga aatatcaatc ttgcggcaat cgatcagtta gcttttgtcc
    14221 tttctgatct tagaaatcgg ggaaaggaag tgatcttagt ctcatcagga gctgtaggcg
    14281 ttggtatgca taaattacat ttgtcagaac gtccgacttt gattcctgaa cagcaagctg
    14341 cggcggctgt tggtcaagcc gaactgatga atatctataa tcaaagattc agtgtatata
    14401 atcaacagac ggcacagatc ctcgtgacaa gagacgtggt agattatcct gaaagtcgta
    14461 aaaatgtgag gaatacattg gaacagcttt tgattatgga cacgatccct atcgtgaacg
    14521 aaaacgatac tgtagcagtg gatgaattag atcatctgac aaaatttggc gataatgatc
    14581 agctgtcagc catcatggca aaacttgtcg cggcagacct tttgatcgtc ctttctgata
    14641 tcgacgggtt ttattcagat aatccgacta caaatcccga ggctcagatg tatcgctata
    14701 tcgataaaat cgatgaaaag attctggcac aagcaggtgg tgctggtagc acatttggaa
    14761 caggcggtat gcagagtaaa ttaaaagccg cttctaggat atttgaagca aacagcgcaa
    14821 tgattttagc aaatggaaaa aatccggcaa ttatttttga tattcttgca ggaaaagaag
    14881 taggtacttt atttaaggag gacttgaaat gactgacttg atgcaattag gacaacaggc
    14941 aaaagatagc gcgcaacaac tagcacttat gcctacaaac cgcaaaaatg agctacttgt
    15001 tcaaatggca caaaccatca aaaagaatca acaagcgatc attgaagcaa atcataaaga
    15061 cttagaaaaa gcagtcgaaa acaatatcag tgaaacgatg caagaccgct tgcagctgac
    15121 acctgagcga atcaatgcca tggctacaga gatcgaaaaa attgcttcgt tagacgatcc
    15181 tattggaaaa gtagatgaga tgtggacgaa caccgacgga ttgagaatcg gcaaaaagcg
    15241 cgtgccacta ggcgtgaccg gcatcattta cgaatctcgt ccaaatgtca cgacagatgc
    15301 agctagtcta gcattcaaat cagggaatgc cgttatcctt cgcggtggca aagaagcatt
    15361 cttctcgaat caattattag tccaattatt gcagcaagta ctggtcagtg ctggcgaatc
    15421 tccttatgcc atccagtttg tcgatgatac ttctcatgaa actgcaggac agttgatgcg
    15481 attgacagaa tacttagatg tgctgattcc tcgaggcgga gcaaaattaa tccaacgagt
    15541 caaagaacag gcaacggtac ctatcattga aacaggtaca ggaaataatc acgtatatat
    15601 cgataaagaa gcacagttat ctatggcagt gaacatcatc gtcaatgcaa aagcttcccg
    15661 tccatctgtc tgtaacgcgg cagaaacatt gctgatccat tctgagatcg cacctttttt
    15721 cttgccagct attgaaaaag aactggtaga acacggggtc tctttacgtg cggatgctcg
    15781 tgcgttagaa tatctagaga cggctgcttt agcagaagac acagattggg atactgaata
    15841 tctagattat atccttgctg tcaaagtggt cgattctttg gatgaagcaa ttgcacatat
    15901 caatcggcat aatactaaac attcagaaac gatcgtgaca gatagttatg cggctagtca
    15961 acgtttctta aatgaagtgg acgcggcggc tgtctatgtc aatgcctcca ctcgttttac
    16021 agacggatcg gtatttggtt ttggtgcaga gattggtatc tctactcaaa agttgcatgc
    16081 ccgtggtcca atggggttaa atgaattaac ttccacaaaa tatatcattt atggcgatgg
    16141 acaaatccgc gaataatgtt tcgtgtgaaa cattattccc gccttttgcc ttttttctgt
    16201 aaaggttcta tccaactaga aaggtgtcaa tatgtttgaa attttaatga ttttttttat
    16261 ttaccttatt tctctgaata tcgctgcttt tctgggagtc ggtattttat cccttttctt
    16321 tcaattcaaa aaacgaagca tagggagtca acgggaaaaa tggtcgcagt attttgacaa
    16381 aatcgggcaa aaagggctgg ttacacgctt gcacattagt tacatggttg cattatgctt
    16441 attagcgaca atcaattact attctttttt cgatcattca atcgcttata cgattacgct
    16501 actgattgct gggatttttc acttatctta taaatatcag ctgaataaaa accacttgaa
    16561 tcggactttc cgctaaaaaa tatccgtacg ataagtagag tgccttgaag atcggcaaaa
    16621 aactgccgga ctttttagag gctgtgacaa agattttgtc acagcctctt tttccatata
    16681 aacggcgttc gcctaccagc tgcctggtaa atctgtttcc tcttcgttaa gaagcgaaaa
    16741 tccgtcaaat gtttcgccat cataataacg aagcagctca tccatggaca agttcatcgt
    16801 tttttctttt tcttcttccg ctgattcagc cagtagctgt tcccgataca cctgatacat
    16861 accagctatt ttttgctgca tcgcttttct agtttcacct tccgcattga ttattggcaa
    16921 ttctttgata tagccacgat aaatcaaccg atcatctgaa aattcaatgg gtaattcaac
    16981 gagtgtcaac ccgacttcct gaaaatattc ttccagcatc tattatcccc ccattctttt
    17041 tcttaaatga attttagctt acctactgaa aattttccag tttcattatt ttgagaaaaa
    17101 aagggaatgt gacaaaaatc gtgtcacatc ccctttttcc gaataaatgg tgttcttctg
    17161 ccaactcctc aatcataagt cacaatattc aacaaacatg agaaactgtt ttttgaaatt
    17221 gttccttgtg attcagagtt aaacgacagt ctcacaacct cttggtaaac ggcgttcaaa
    17281 agcttgcatc tgcggtaata agcccaacca aacgaaaaat atgagaagct atttttgttt
    17341 ggctgttctt atgacttgat gcaggacagc tttttcacaa cctctgattt cttattcttc
    17401 ttcatttgta gcaaaataat tacggtgata aatgatacaa catacactga taagaacaat
    17461 ggcacctgca aaataaaacg catagctttc accaatatgt tcaccaatca cacctgcaat
    17521 aaatggtgct aaagctccac cgaaaaagcg gacaaaacta tagctggacg aagcaacgtt
    17581 tcgttgcaga cctggtattt ccatagcaat cgtcgtcaac agtgtattga taattccttg
    17641 gaaaaatcct gctaacacaa tgctgatcgg tacaaggact ggcatagaag ttccagctcc
    17701 taataacagc aaacaaataa tgaataagac aaatgctgct aaaattgtcc gataagtcgt
    17761 aaacttccgc tcaagagccg gtgcaacgaa aatcgaagca atagccagaa gcacacccca
    17821 gccaaaaaat acaaaaccga cttctaattc agaatagcca atcaataaaa atggcgcata
    17881 cgcaagtaaa gtaaagaatc cgaaattata taacaatgcg attagtccaa ctgaacgaag
    17941 tttatgattg cttaacgctt tgacaccttc ccaaaggcct acttttctct ttggtttctc
    18001 agtttcaggt aaaagtaccg ctagggctat aaaaccgcaa aacatcaaaa atgctacccc
    18061 aaaaaatgga taacgccatg aaccactgcc caagacccca ccgactagag ggccaacagc
    18121 cattccgcag ccaagtgctg cttcatacat catgattgct ttttctgtgt taccaactaa
    18181 aatactgacg atcgttgcta aagcagtaga gacaaataaa gcattcccga caccccagcc
    18241 ggcacgtaac ccaatcagca ttccgatatt cccagataat ccgccaagac cagcgaatac
    18301 aacgatgata aacaatcctg tcatcaatgt tttttcaaac cgatccgact agataagaaa
    18361 cctgtaaata gcatcaccac accagtgact aacatgtaac tagtaaataa cagggtcgtt
    18421 tcagctggcg tagcgtttaa ctgtgcagca atcgttttta agattgggtc tactagaccc
    18481 actcccataa aagcgatcat acaggtagct gctacaacta gcgctgaact tttccccttt
    18541 gtttcttcat ttgtttgttc ttgtatatct atttgattct ccaatatttc catccctttc
    18601 tctctattag catcatacat gatttatttt acatgtaaat atgcacatgt aaaacgtgga
    18661 aactttactg tgatataata aagaaaataa gacgagaggt aggattttta tgaatacttt
    18721 agcctatata ttattatgcg tgcttttatc caaaccacgt tctgggtatg aattgaagca
    18781 gctgatcaca attttttggg aagcccatca cagtcagatc tatacaacgc ttgccaagtt
    18841 agaccagcag ggttatgtga ccgttttgga caaagacgag cactctcaaa aaaagatcta
    18901 ccacttgact gatgaaggac gtgctcttgt agaggaatgg atcaaagagg aaactcccgc
    18961 tcctacgcaa aaagatgaat ttctagcaaa aatctatgct ttttcgacgc tcgataaaac
    19021 aacagctact ggactgctat tcactagaga acaacagctg aacaaaatca tgcaaaaaaa
    19081 tcaaaaaaaa ttgaatgacc tctcgctaga caagaaacaa gaatttggac gttatgttgt
    19141 gatccagcgc agaattatgc tttgtcagca ggaactccaa tggtgtcagc aagtaagaga
    19201 acaaatgcag cagttttttg agtaagggat aaacagaacc tttctagaaa tatctattga
    19261 agagcaaatt atcttcaata aaagagcaat acagttggaa aagaaaaagc gatccgaacg
    19321 acaggccaaa acctatcgtt cggatcacta tttttctaga actgcattca agagactatg
    19381 acaaaaagca tgccaaggcc ttttttcaaa taacagtgtt ccatcattca ttactttgaa
    19441 ttgcttttag cacacttctt gaaagcgggc caacgatgat caactgcaac ggtaaagcgg
    19501 caatcacatt cattttccaa gcattcatat aagcagagaa taattgatca ggtattcctt
    19561 gttccatgat cacaccgaat aaagacatac atgtcaccat tcctaaaacc atcaagcatg
    19621 aaatagttaa gatcatttgg accttgcttt ctggatttac tggtagttta aaggcaattt
    19681 ttttcgccag cacaccgatg atgaagacat ccaaaatgaa agccaccacg aatccaggca
    19741 ctagaccaat caacaaactt gataaatgaa gatcttgatg cagccataaa ttatacgcgc
    19801 tcatccccaa gaccatcaaa aaacacatca ttgttgtaaa aattattcct tcttttttat
    19861 ttgtaggcaa tctgtaccac tcctttaaat ttgtcacgaa aatcatcgta gcacatttaa
    19921 aaatttctcg taagtacttt ttttattttc tgccctttta tttcataaac ttcatcagcc
    19981 gtacgctcga caaagtaaga atcatgagaa gtaaacaaaa tcgttcccgg atatatttgc
    20041 attagcttct ctaaagcttc gatcgttgcc atatcaataa aattagtcgg ttcatctaac
    20101 agcaacacat tacttggctt agtaaaaagc aaagcaatcg tcagacgggt cgcttctccc
    20161 ccacttaatg tacataacgg tttcgtcaaa gtttcctgag caaaccctaa gtgattcagc
    20221 aaactacgga caagcgattc tgaaaactct gtttgtctca tgacaaaacg aatgatggat
    20281 tcttcagatg tcatttgata acccttttgc tgatatactt gaaagcttac tttcggagag
    20341 agaaggattc cttctccttg tttgcggata tgttctaata aggttgtctt acctgaacca
    20401 ttttcaccga caatcgcgat ttttttacct aaagaaaact gaaaatcacc atttaccagt
    20461 aatgttctcc cacttctttc caattggaca ttttgtccca tgatgggata gcggctgtgg
    20521 agttcaagag attttggtac tggaaaacgg atttgtttca tctgctgcgg cttggttgtt
    20581 tctcctattt tttggagtct tctctctaat gttttcgcat tcttttggat ggctttttgt
    20641 acactgtctt tttgtttaga ggaagacaac cgatccggtc tgatttgttg ctttttcttt
    20701 ttactggaca ctttttccaa cttttccgct tgttttttct tttcttggat cgatttttcc
    20761 aatcgttttt tctctttcat gatcgactct gcttctcgta gctgagtctt tttctccaat
    20821 tccttttgag tgagataggc actataattc cccgaaaact ctcggatttc tccgtctttt
    20881 acttcccaga ttttcgatgc tagctcatct agaaaatagc gatcatgact aacaaaaacc
    20941 agcgtaccat aataataacg aagctcttca atcagtcgat caatactttt tctatctaga
    21001 tgggttgttg gctcgtctag aagcatccct tgttcataag aagaaagaag acgtatcaac
    21061 tcgatctttc gttcttcccc tccgctccat ttccgctctg aagtttcttc cagtcttaac
    21121 tggaaatgac tggctaattc caaatttagg tcctttgctt cagcgagata ggttaattga
    21181 ggcaggtagt tgaaggtgat ctctgtttgg attttcccat gatcgggaaa aagttcaccg
    21241 gctattagtt tgagcaaagt cgatttaccg acaccatttt ctcctatgat ccctatacga
    21301 tcttgctgat agacacgaag agaatcaata gatagttcta gctggttgcc aaagctgact
    21361 tgtagatttg ttatatttac tgctaaattt tccatatgtt ttacactcct atttcatgta
    21421 aaacaacaat tatcgatact aaaaaagagc agaaaaatct gtttgcaaaa aaactgcatc
    21481 acatcttttc ctactctgaa cgaaataact atgactaaat caccaaatct gtcatagctt
    21541 tcacctattc tactgtcgtt cgaaaaaatg gacatactcc atccatgaaa ttgttgttta
    21601 tacatgtctt ttgaatttat ttagttgtta ttcaaaagtt cgaaacgtaa tttcatcgat
    21661 gcagtcattt ttccgacctc cttttttatt aggttaccac acacgattca aaaaaagcaa
    21721 tagctactga atagtaactg aaaacctttt attcgaacgt agtgatcttt tctggtgcta
    21781 aacgaacaga ctcgtaacct tcttctaccg cttttcctat tgataaaata ataactggaa
    21841 cataacggtc tttatccaaa ccaaatgcct ctgccaactg atctgcttcg aatccaccaa
    21901 ttggatttgt ttcatagcca tgatcacgcg caactaacat gaattgcata gccgctaagc
    21961 tagaatcgac tttgactaca tcgttcattt cttctcttga aaaactcttg taataaggaa
    22021 tgatcgcagc taattgttga tctctgactt cttttggcat cttccctgat tcgtaagctt
    22081 ggttatagat ttcttcgcca tattcgtaac attgcatatc gccaaaaatc atgaccattg
    22141 cagaagaagt atcattttgt ctcgtattga aacggatcaa aggacgcaag atttcttttg
    22201 cttcctcgct ttcagcgacc acaaagcgcc atggttgcat attgacagaa gaaggtgcag
    22261 tcgtcgcttc tttgatcatt tccagcattt cttcgtgtgg gattttgtat gtttcatcat
    22321 atacgcgaac ggatcttcta ccagtcataa ttgttgaaaa atcatttttt tgttccatta
    22381 ttattaacct ctttcgtttc tgttgttata ttgcaaagtt gggtattcaa tttatctaaa
    22441 agtgttagaa attgtttcat ttctacatcg ctgaaagccg gggatagcac ggaacctgta
    22501 tctttatggt ccatttcaca atgctcgatt ttcttctggg ctttttctgt tagcgctaca
    22561 tacacttccc ggttgttctg aggatttctc tgacgcgaaa catagccatt tgcttccaat
    22621 accttcagat gacgagtaac tgctccttga tcaatctgca acttttcttg caaactgctt
    22681 tgtagcaatg gattttcttc tttcagtctc atcatgatct cgtatctagt aagacttata
    22741 ccgatatttt cttcaaagat ctggacgatt ttttgatcgg ctaatttgat ttgatataaa
    22801 aggtgactaa tctcttctaa agacataatt tcctcctttc taataattga ctcgtcaacg
    22861 attgacttgt caataataga ataaaaaaag aaaaaagtca aagaatcagc tgtgtaataa
    22921 aaaaaacgct ctcaatagaa aagtatagag aaaagaaatt ttttgtagaa tgaacggaaa
    22981 aaatgataaa gaaagcacaa cgctaaaacc aagattattg attcttatca aactatagaa
    23041 gatagctctt ctgcagtaga aaattttaca ccgccaaaaa tttccgaaat cgccttttgc
    23101 aagaatcccg tcgaatatta gaaattggat cagtatgctt aaacagtaat aaaaaagtta
    23161 tgacaagatg ttgtcacaac cttttttgag taaacggtgt tcaaaaactt gcatctgcgg
    23221 taataagttc aaccaaacaa aaaacatgaa aatctgtttt tgtttggctg ttcttactac
    23281 ttgatgcagg gcagcttttt cactacctct ttttaataac cataaaatcc tattccttca
    23341 tatctccagc ccatcggtgt tagccaatct ctttccttag cactcaaggt atagtgatgg
    23401 gtaccagttt tttcatttgg attaaatagt cgataaattg gcttggaacc accagaataa
    23461 aaacttagac cttcataacg ccaaccgatt tttgttaaag agttgatctc agattgtgcc
    23521 attgtgtaat gatggtctcc actgtaagga ttgtataaac ggtagacagg ttgtccgctg
    23581 tttggtgctt gccaagcaat cccttcatat tcccagccta cagttggcag ataatttttt
    23641 tctccggcat tcaatgtata taaatgttct cctgtatgac ggttatacag acgatacatc
    23701 ggagaagttg aagttggtgc tgtatcatac tgtgtcagat tataggtttc gattacattg
    23761 ttcaatgtag aaccataatt aggcgcagtg gcataacgac ctgttagcca agccgtagca
    23821 tctttgtaag agttcgtatt gcttttccat gcaccggaat agtagtaaac gcctggctga
    23881 aaagaagtcg tcttcaatac cgttacgtta tcttgcaatg attctttgta cgaaggatat
    23941 tttctaaaag cggcattcac cgtaatccat tgcccattca catattccga agtaggcatc
    24001 gtaacagatt ggccattata gcttccttta atcccaaata gattgtaatt aggggcagaa
    24061 gccaaagtac tgttccccca gccactttca acgatggctt gagcaatcat gactgatgca
    24121 tataagtcat tggcacttgc caaactttgt gcagttgagg caatactatc aatgaacgct
    24181 tgttgagaat tagcagatga gcgagcataa aaagcatagc cagtatagtt ttcgaattca
    24241 gacaaatctg cttgatgggt atgtgtcaat gtttgcccat ctgtcaattg gttcgtgatt
    24301 tcttctgcat tctctggatt gttctgagct tctgctgatg tactgtcttc ggtttgtgtt
    24361 tgttctgttg tttcagttgt tggttcttcg gttgaaattg tattttctga gacagtagct
    24421 gactgcaaag tactctctgt atcgttttca attcctgttt ctgaagaact cgatgtctga
    24481 gttgcgacag tttctgtcga ttcttcagct agcactgttg tgttggcaac tgctagattt
    24541 cctaaaagaa taacgctaaa taataaattc tttgctttca tcttttttct cctttgaatt
    24601 atgaaacttt tcctaaataa cagtgtaact aaaaaagtaa aagcaagtca atgtccatat
    24661 ttaaataaca tttgtttctt tttgataaat ttgctttttc tacataaaaa gacgtaaatg
    24721 aagaatattc ttctccattc acgccttaaa ttgatggtcg tttgaataac tatttaacaa
    24781 gtaattctct ttggatatta tccgttatgg attatacttc ctgtgtcgtc ggacggataa
    24841 tcatgttact gacagacatc cggtctggcg tttctatcgc aaatgctaca gcagaagcta
    24901 tatcttcgga ggtcaatccc cattcttttt gcgcttcatg aagttcaaga gcagcctttt
    24961 tgtcgctgat ggtttgatat aattcagttt gaacagctcc tggagagatg atcgtagatt
    25021 tgatgttatt ttgccgttgc tcttgacgta atccttccat gattgcacgg acagcaaatt
    25081 ttgttccgca ataaacggcg gaatcaggat aaacaacatg tcccgccaca gaatcagttg
    25141 aaatgatatg cccagatttt tgtttttcca tgattggaag tacggcggaa attccattta
    25201 acacacccat gatgttgata tcaagcatat tttgccattc atcacggtga ccttcaacta
    25261 atggagcggt tggcatgatt cctgcattat tgtacaatac atcgattcga ccgaactttt
    25321 ccatcgctaa atcgataact gcttggactt gtgcaaaatc tcttacatct gctgtttgaa
    25381 tatatagctc agcttctggt aaagattctt ttattgcttt taggcgatct tctcggcgtg
    25441 cagctataac tagtttagcc cctttttctg ctaatagctt cgttgttgct tcaccgattc
    25501 cgcttgaagc gcccatgatg acaatcactt tttctgataa atcattcatt ctattctcct
    25561 cctgttttga aaaatctctt gcttatactc actatctctt atttcatctt actgttcaaa
    25621 taatccgttc gacaggtttg acgattttgg cagggattcc tgcaacgatg gtattgtctg
    25681 ggacatcttt cgtaacagtt gcattcgctg caacaatggc attctcgcca atcgttacgc
    25741 ctggcagaat agttgctcct gctccgatcc aagcattttt cttgatgtgg attggcttta
    25801 ctgacaagcc tcttcgttct tttggatcgg ttagatggtt caccgtgatg aggttgacac
    25861 gaggaccaat caatactgaa tcttctaaac agatgccacc taagtcaacg aacatcactt
    25921 gacgattgat aaagacattt ttccctatac taatatgttt gccgaaatct gtataaaaag
    25981 gcagcagaac agtcaccgta tcatctactt tgtcactaat gatattttct agccgtttgc
    26041 ggatatctgc tggttcatgg tacccagtat tcagcgctgc taaaggctct tcgttttcag
    26101 cttggatctg atggatcact tcaaagagcg gatccgttac tcgaatctct ttattgacga
    26161 tttgtttttc gatgaggctt gtcattttgc tccctccttt tatccttact ttataacgga
    26221 cggcttatca tgtctaatac ttataaaaca tacttaacta ttccttttaa ctatacttag
    26281 tggtatgctt ttttaaaaac aggagggaaa aaatggatat tcgtatactg acgtattttt
    26341 tagcagtagc tagagaaaaa aatatcagcc gagcagcaaa atctctgcat atcacgcaac
    26401 ctactctctc aaaacaactg aaagatttag aagaagaact tggtgtgaca cttttcactc
    26461 ggggctcccg agagatacag ttgacggatt ctggtcgata tctttattca aaaggaaaag
    26521 aaatcttgtc gttggtagaa aaaacggcga acaatttaac aaatgacgca atcatctctg
    26581 gagaaatcta cattggcgga ggcgaaacta aagcaatctc tttcatttct gatagccttc
    26641 attcgttgat caaggcgcat cctgatatcc agtttcattt atatagcggg aatgcagatg
    26701 atatcataga caaactggat agcggattac ttgattttgg actcgtaatc gaaccgacaa
    26761 acaaacaaaa ataccagtca tttcgactgc cagcccaaga tcgttgggga cttttgatac
    26821 atcgaaatca tcctttagca aagcaaaccg tcatccatcc gcaggaactg aaaactgttc
    26881 ctttgatcat ttcccggcaa acgtcagtgg acagtcaact agctgaatgg ctaggtttca
    26941 gcatagatca gttatcgatc gtcggaacat ataacttatt gtacaatgct tctttgatgg
    27001 tcgataacgg gacttttggt gcattagctc tagatgggat catcaatact tatgggacgg
    27061 aaaaaatatt catcccactc gatccgccat tacaagcttc actgaatctc atttggaaaa
    27121 aagaccagcc tctttctcct gctgctcgta aatttttaga tgtcctaacg gagaaataca
    27181 gttaagtaga gtttttttcc ttcgctttca gctttttaac aacgaataag ataagcaaaa
    27241 gatccaacga tta
//
LOCUS       DAXWOO010000021        26265 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.021, whole genome shotgun sequence.
ACCESSION   DAXWOO010000021 DAXWOO010000000
VERSION     DAXWOO010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.021"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(<1..1541)
                     /locus_tag="ACGE70_000727"
     CDS             complement(<1..1541)
                     /locus_tag="ACGE70_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFF8692382.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEF"
     gene            complement(1545..1955)
                     /locus_tag="ACGE70_000728"
     CDS             complement(1545..1955)
                     /locus_tag="ACGE70_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFF8692383.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(1952..2875)
                     /locus_tag="ACGE70_000729"
     CDS             complement(1952..2875)
                     /locus_tag="ACGE70_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFF8692384.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(2872..3750)
                     /locus_tag="ACGE70_000730"
     CDS             complement(2872..3750)
                     /locus_tag="ACGE70_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFF8692385.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(3737..5074)
                     /locus_tag="ACGE70_000731"
     CDS             complement(3737..5074)
                     /locus_tag="ACGE70_000731"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFF8692386.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(5077..6180)
                     /locus_tag="ACGE70_000732"
     CDS             complement(5077..6180)
                     /locus_tag="ACGE70_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFF8692387.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(6254..7762)
                     /locus_tag="ACGE70_000733"
     CDS             complement(6254..7762)
                     /locus_tag="ACGE70_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFF8692388.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(7728..8420)
                     /locus_tag="ACGE70_000734"
     CDS             complement(7728..8420)
                     /locus_tag="ACGE70_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFF8692389.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(8442..9416)
                     /locus_tag="ACGE70_000735"
     CDS             complement(8442..9416)
                     /locus_tag="ACGE70_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8692390.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            9716..10588
                     /locus_tag="ACGE70_000736"
     CDS             9716..10588
                     /locus_tag="ACGE70_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8692391.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(11046..13244)
                     /locus_tag="ACGE70_000737"
     CDS             complement(11046..13244)
                     /locus_tag="ACGE70_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8692392.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            13476..14468
                     /locus_tag="ACGE70_000738"
     CDS             13476..14468
                     /locus_tag="ACGE70_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8692393.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            14528..15949
                     /locus_tag="ACGE70_000739"
     CDS             14528..15949
                     /locus_tag="ACGE70_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFF8692394.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            15942..17441
                     /locus_tag="ACGE70_000740"
     CDS             15942..17441
                     /locus_tag="ACGE70_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFF8692395.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            17480..18874
                     /gene="uxaC"
                     /locus_tag="ACGE70_000741"
     CDS             17480..18874
                     /gene="uxaC"
                     /locus_tag="ACGE70_000741"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFF8692396.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            19033..19716
                     /locus_tag="ACGE70_000742"
     CDS             19033..19716
                     /locus_tag="ACGE70_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFF8692397.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            19718..20554
                     /locus_tag="ACGE70_000743"
     CDS             19718..20554
                     /locus_tag="ACGE70_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFF8692398.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            20565..22319
                     /locus_tag="ACGE70_000744"
     CDS             20565..22319
                     /locus_tag="ACGE70_000744"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8692399.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(22595..22936)
                     /locus_tag="ACGE70_000745"
     CDS             complement(22595..22936)
                     /locus_tag="ACGE70_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFF8692400.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(23004..25175)
                     /locus_tag="ACGE70_000746"
     CDS             complement(23004..25175)
                     /locus_tag="ACGE70_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFF8692401.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            25386..26243
                     /locus_tag="ACGE70_000747"
     CDS             25386..26243
                     /locus_tag="ACGE70_000747"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFF8692402.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
BASE COUNT         7816 a         5401 c         4728 g         8320 t
ORIGIN      
        1 aaaaactcaa aatcagcata acttcctctt tttttattta gatcatgcac tgctatcccg
       61 ataaaatttc cagtaaaccc tcctgagagt atacacatgt ttttttgcgg acctactttc
      121 ttccattctt cagttgatac gtctttcaaa tgaaactgtg ctgtctcttt ctttacagtg
      181 actttcaaaa aagtcttctt ttctgtgagt ggaatcgtcc aatcttctat ttgtgctact
      241 ccgttttctg attctatcat acgtaggcat tttcccttgt cctcattcca agtaacataa
      301 caataaatat aattggattc atttaagtac agtaccagtc cagccatctg attaaaatta
      361 ttaggcatgt attcaaatgc tgtctccgca tcaaaagtaa aatccgtttg ccgaatagct
      421 aacaaatgat gttcgaaggt agattgaaga gattcaccgg aaatcagacg taaaaaacct
      481 ggtctacatt ttgtatcaca ccaatcagca tttggcaaaa tgcgtctggc attccagttc
      541 ttctttagct ctccttcaaa tgtatccata aactgatgat ctttttcttg ttgtacctct
      601 tcatttgtct caatcattat ttcttccatc ggagttgtct ggctatcttt aagtcttaac
      661 caatcgtcgg acgtccaata cacttcttga atagcggttt ctcttcccaa aatagccgca
      721 ccgtttaatg gtctggtaca aagatatgtc atgtaccatc ttcctgacgg ggtttgtact
      781 aagctcccat gtcctgtaca ttgcaatgta gaagcaggtt tgtcactagc tgtcagcata
      841 ggaaaaccag gatcaagctc gtaaggacca gtaatattct ttgctcggca aactgtaact
      901 gaatgtcctc ttcctgtacc gccttctgcc gtcatcaaat aataataccc gttacgaaaa
      961 tacaaatgtg gtgcctccgt tttcgctaat tctgtacctg caaatatttt atagatggga
     1021 ccatcaagct tcttcttctc tgcatcatat tcttgtaata cgataccgct ggatttattc
     1081 ggtgtgtcca gacgataatc ccatagttca ttgacaagcc atttttttcc cgtttcaaga
     1141 tcatggaaca aagaaggatc aaagccgtta ctatttaaat aaattggttc ggaccaagga
     1201 cctgtgatct cagatgcagt gatcaaataa ttgtggacat ctttaaacgg acggcctgca
     1261 cttttgacat ctgtgtaaac aagataaaac aatccatcag cataactgat ctgtggcgcc
     1321 catatacttc catttgtagg atttccttgc aaagatactt gagtggtcaa aatatccgtt
     1381 gtatgtgacc agttgactaa atttttcgac tcatatactc ggattcctgg aagccattca
     1441 aaagaggaga caataatgta ataccgctct ttcactctta atatattagg atctggattg
     1501 aatcctctta aaataggatt tttcactttg atagtctgca tgattcaggc tccttttctc
     1561 attcttactc gatataaact gaatgcccca gctgcgaaat aaaaaaatgc aaaaggaaac
     1621 gcaataaact gagtgccaat caacaatatg aatcctgtta taaaatacaa accagtggca
     1681 gttgttaagt attttccttt agcagtcaaa aacaatgcta ctgctaaaaa agcaagtgaa
     1741 ataaacaaca caacggcaaa cagaccagtc atgtcctgta ataatccaaa tgtattttct
     1801 aaagacaaag ttgaatgatc tacagcaagc gactgataac tttttttaaa ctctgacgca
     1861 gacatattac ttaaagtaac tgctaacccg ccaaagaaaa tcaaagatag actgccaccg
     1921 accattccta gcaatagttc ccattttctt ttcatttctg aaccccctgc ttttctaaat
     1981 gtatccattc aggtcttttg atcgatgttt cataattgtt attacgttca gagaatttga
     2041 cagtctgttc atttttcttg tcatcccaat tatcccaacc agttgggtga acatgcccac
     2101 ctatcttaca cgcttcaaat aagacatttg catacggacg ccacggtctt cctaaataga
     2161 cgtttctagt atcaggttct gcagtcaagg tacagctttc aaaacgatac cctgtttttt
     2221 gatttttcgg cgtagatgct gctgtgatgt atccaggatt tttactggct aagctatgga
     2281 tatgacactg atgaaaaacg gcatctgccc caccaaatat gaaatccacc gttccttcaa
     2341 tccagcaata ttcataggtc tgacgacaat actcaggatt ggcagaagcc ggtgtacaaa
     2401 aaggagtacc atctttttgc aaatcaggca acggacccgt gcataaggta tcctgataac
     2461 cagatagtcg acagtttttt aaagatacgc gatctcctga attgaataaa gcaacagcct
     2521 gtcctacctt ttctcctgga cctgctgtat tttcgatgtg aagattttct aatacgacat
     2581 tcgatccttc taagaaaaaa gtagcacttc ggaaagtccc taactggcta ccgtcaagat
     2641 actcctgttt tgcaaaacga ttaccggaga taactactgt tcctgctccc acaagtttaa
     2701 aattggataa tctgctttct atatattcat catatcttcc ttcttttact atcaagacct
     2761 ttggatggct atccggcagg cctgccaaat aatcgattcc ttcttggata gtttcaaaat
     2821 ccgtcggggt atctatagca ttctttccaa ttatcagttt gtctttcatc ctcataaatg
     2881 ctcccttatg aattgtttcc aaatatttct catttcttgt gtttccatat gaccgcctgt
     2941 taaagaaaaa gaagaaaagt tttctggact acccattgat tgatactttt tgtccagccc
     3001 ttttctcagc ttcagtacgc cttcgtccaa gcacatcgta tcgttttttc caactaagct
     3061 aagacgaggg cgcggagcga ttttttcttg gattgctaac gttgtataag ctgtcaatag
     3121 atttggcaca tagtaataaa acccatgatg atctaggcgg cgatgtagca aaagtatttc
     3181 gatgtccacc tgtgccgcta gatcaataca aactttgaca cgagaatcca atgctgtcac
     3241 ccaccagctt aataagcctc ccatagaaag accaatcgtt gcgagccgtt gagtatctgt
     3301 gtcttccctt gtctccaaat aatcgatcaa gctgatcaca tcataaatac gcattcccca
     3361 taacgttttt cctgttaata aaaattgttt gaacaactcg ctttcagaaa tacctccgcg
     3421 ttcttcaaat ccccaagcat caatcgacca aacagcatat cccattcctg tcagtacttc
     3481 tgcaaaagat ggagaagcta agtaaggagc accatggatc aattcacttt tcccgactga
     3541 aaaatctccg ccgtgagaat gcaggtaaag gaccggcgga aacggtcctt ctttttctgg
     3601 ataagcatag agggctggta ctgtttccaa ttcatttaag tacaattgat agtattcaag
     3661 cataaaaccg ttttgttttt ctcggtataa tcgttttcca ctaggggtat gtctgctaga
     3721 tggagcacct aaaaaatcat tcaactgcat aagcaaagtt tttacgaaca accagttcat
     3781 ccatattttc tgattgattt gattggaatt tctcaatact gatttcttta gtattttcta
     3841 atagaaatgc tggtccctcg tgattttcaa tcgacatctg gttaaaggaa accttacccg
     3901 caaagccaat ataaaatcct tgtctgctca tttcctctat tcctgtcatc atagcaggaa
     3961 caccgggaac tgcatcttgg gccatcgaga catcgatatt gtcaaatgtg atttcttgta
     4021 tgaattgttc cgctaatcca taaataaaac ctgcagaagc atggacattc cttgccgtga
     4081 tattcgaaaa atggatcctt ctgaatgctg gtgtccgttc atctatcgga taagcctttt
     4141 tttcccatac atacggttct ttccctcgcg gaccacagaa ataataaaga ttcagtataa
     4201 atggacacat cacattatcc atgactatat tggatactcg aatatcttca acgatcccgc
     4261 ctcgacctcg tctagatttc aatcgtattc ctcgatcagt ttcttggaag atacaatttg
     4321 aaatggtaat attacgaata ctaccactca tttcacttcc taatacgaca ccgccatgtc
     4381 catggaccat cgtgcaattt gtgatcgtga tattttcgca agcgatcctt tcgtacgtat
     4441 cttcagtccc tgccttgatt gcaatacagt catctcccac gtcaatatgg caattactaa
     4501 tacgcacatt tttgcatgat tctggatcaa ttccgtctgt atttggtgaa tccgctgggt
     4561 tcaagatcgt caagttatca aacgttgcat tactgcaaag aatcgggttg atcgtccaac
     4621 ttggtgactg gatcaattta atatctttca cagtaattcg atgacagtta tggaaactca
     4681 tcaatttagg acgaggataa gcgagattat ctggttcatt tctaaaagta tgccaccatt
     4741 tttttccatt tccatctaat gtaccgaaac cagtgacaga gatgttctct acattttgag
     4801 catagataca ggaagcataa acttttcgat ggactccttc ccaacgtgaa tgaacaacag
     4861 gataatcttt tggatcgtca gagaatttca gaaccgctcc tgcgcttaaa tgaagttcga
     4921 cattgctttt taaaaataaa gctcctgtta gaaattcacc tgctggaaca actacggtcc
     4981 cgcctccgtc acttgctgca gcatcaatgg cttgttgtat tgcttcagta ttcaattcat
     5041 caattgatgc gccaaatgtc aaaatatcat acatatttat aatgcctcca cttcaatcaa
     5101 tgcttgcaag aatgcaccaa cgcctttttg atcattacaa ataattggtt cactgatgta
     5161 gtaagcatat gaaccatctc gctgatctgc tccgcctaac cctgctactt gacagttttt
     5221 attcaagttc agccagcctt cttttgtttc cagaacaaat tcatcaagta atccttgatg
     5281 tgatttcttc acttgtggaa gccattcttc tttgttcagc agtcccaaac gtattccttt
     5341 agccattgcg taaacaaaca tactactgcc ggaagcttcc agataatttc ctttccgttt
     5401 tgtttctgtt gttacttgat accatgtccc gctgttttct tcttgatatg gaagtaatgc
     5461 agccagtgtc ttttctagaa tttctttcag caaagaacaa tctgtttctt catgaagtaa
     5521 ttcataggta tctgctgcag ccatcaaata ccagccaatc gatcggctcc aaaaatgatg
     5581 ggatagacct gtttttggat cagcccatgg ttggaccttc ttttcatccc atgcatgaat
     5641 cagcaaattt gtttttggat cgaatagatg tttataactg atttcaaact gtctcaaaac
     5701 atcttggtaa tctttttctt cactaaaatt ctggataaat tcagcataaa aaggagaacc
     5761 catatataag ccatccaacc aaatttgata aggataaatt tctttatgcc aaaaagctcc
     5821 ttcggatgtg cgtggatggt ttgccaactg ctgggaaagg agttctgctg cttttttata
     5881 tttttcttcg cctgtttctt tgtacaataa aaagaaaagc tttcctgtat ttacatggtc
     5941 gatattgtat tcttcaacgc tgtagccacg gatcgttcca tcttcttgca cgaaatagtc
     6001 gatattgtct tttatgaaat caaaataaat tttttcttgg gttcgttccc aaagtaattg
     6061 gaacccttta agtacgacgc catagtcata agaccatttt cctttatacc acttttcttc
     6121 atataagcga ggtgttcttt ccatgatact ttttgctaat ttctttgacc agttttccat
     6181 tatttgctct cctttaaaaa ttaaaatagg atttgggaca aataccaaga agtatttgtc
     6241 ccaatcgtct atattactgt gccgctgctt tgtatgcttc ctcgtactct tgtgtgattc
     6301 gatcgcctcc ttgtgctttc cagtcttcta cagctttttt gaatccatct tcgtcaagct
     6361 gtcccatgat gtattgcacc gtagcatcaa tgacgatttt ttcaatttct gatcctactt
     6421 cattgttcgt cggtgaatcc aatggcacag aaggatccat tacagcaaat tcttcatttt
     6481 ctttgatcaa ttgattagag aattcttttt cagggtctgt atctttgaaa gagaacgtca
     6541 cttcactagg acgagagctt gataacggtt gcacctcttg ttgccaaaga tccatatcga
     6601 taaattcgat agaaccatca tcattcaatt cataatgcgt tccttcgata ccgccagtca
     6661 tcaatttata cacatcttct tctaaaagat catcaacaaa ttgaagtaat ttcttcaatt
     6721 gtgcttcgtc ttttacttga gatttgggaa atgctagcaa gccgccaata ccatttcctt
     6781 ctgaccaaac atgataaccg ccgccttcac catctgagat cttgtttaca ggcactaatt
     6841 ccatatcatc ttgaatacct tgacccatcg tcttcagatt cttgatatcc accattccag
     6901 tgtagattcc agttcttcct tgtgcaaatt gttcttgctg atcagtcttt tgggtaactg
     6961 caaagtcttg tgccaaatag ccattctcaa atagatctct tgaaaaattc aaagctttca
     7021 tatactcttc tgtatcaaat tctggagtga atttgccatt atcgtctaca gcccagccat
     7081 ttggtgctcc aaaataagaa gtgaaaagtt taaagctggt ataacgaata tcactaggcg
     7141 cacgatcgcc aaaaccaact gtgtcgtcca caccatttcc atcaggatcg ttttctgtaa
     7201 attgtttagc tacttcataa agctcatcca tggtagttgg aacatctagt cctagattat
     7261 ccaaccagtc ttttcgaatg actaagccag cacgagcgta gtctttttgt atcggtacgc
     7321 cgtacaaggc accgtcgata ctcgcagctt cgattctatc ttcagaaatt tgacttaaat
     7381 ttttatagtc ttttaaatat tctgatacat cccagaatac gcctgatttt aacgcattgc
     7441 ggacagaaga gtttttcaac atcgcaattt gaagtgtaac gatatctcct agttcatcag
     7501 aagccaatgc agttgttaat ctttcttctt ttgaagcatc aggaatccag ttgaatgtca
     7561 gttccgtatc ggttttttct tcgatcaagt tcacaatcgt gtctgtaggc ggtgaagcat
     7621 gatgcaaaat atttaaccaa gtgattttag cttttccatc ttctgtctct gaagaactgt
     7681 tacttgaact gctgccgcat gcggcaagtg ctagaattcc tgcaaaacta agacctaatt
     7741 ttacgagcgt tgcttttttc ataaattttc tccttcactg ttttaataat atttttgttt
     7801 tcttctgttt tttctgtata tgagttcttc cattccaaat ggcaaaatat agaatttgct
     7861 gtccacgtga ctgcggaaaa gaagagactg ccaccgataa aggttaaaac acctggcatt
     7921 gtttggatga tcatcagata aatccctacc atacttaata tcaacactaa tgagtaatga
     7981 agatgtgaga atccgaataa aaatgccatt ttcattttga aaaaatatcc tttccaatca
     8041 taatgagcca tgattgggaa aatatagagt gctgagagca catatatgaa cgcagcaata
     8101 tacaaagcga tcctaagata ccaattgtgt atgaaagaaa gatccacaag taaaacaatc
     8161 ccgatggcgc catatatcca gctcatcagc aaggtttcac gatagttttc tttaaaatat
     8221 gtgaagtacg ttttcgtcaa aggaaacgtt tcttttgagc gcagccattt tctaatcact
     8281 gcaaccatcg catacgatgc aggaccgaaa gtaaaaactc ccaaacctgc gatgactaaa
     8341 ccgccccaca gaaaattcaa ataaaccaac tgtaaaatac gatttagcct tccgtttatt
     8401 ttatctaatg actgcagcat atctttccct ctctttctat cttagtaaac gccgtcttct
     8461 cctagctttt tggcaatctt attagctaaa attaccataa acaatcctac gaccccttta
     8521 aacaatccca ttgctgtact gaaacttaac tgtccattct tcaatccagc tgtataaata
     8581 taggtgtcga aaatttctgc aacgcttcga ttcaatgaat tcaataacaa gtacatatgc
     8641 tcgaacccta agtctaatgt ctgaccgatt tttagaatca gcaaggtaat gattactgga
     8701 cgaattgccg gcaatgtaac atgccatgtt ttgcgtaagc gtcctgctcc atccatttct
     8761 gcagcttcat acaactgtgt atcgactgca gttatcgctg aaaggtagat gatcgtagac
     8821 cagcccaact ctttccagat cacttgtccg atatatactg tgcgtgtcca ttcaggggat
     8881 gttagaaaac tgattttttc aaatcccatt gttgcaagga attcattcaa tacccctccg
     8941 tcaacattca aaaatacata agtaatcgaa acaatgatta cccaagacat gaagtgaggg
     9001 atgtaaatga tcgtttgaat cccgtttttc aatcgtttgt tcgttacttc atttaatagt
     9061 aaagacaaaa tgattggcat cggaaaaaac acgaagatat ttaatccgaa caagatcaac
     9121 gtatttctta ataatgtaaa gaatgtcggt tctgtaaaca aacgaatgaa atgtttgaaa
     9181 ccgacccaag gacttcccat tattcctaaa aatggttgat aatcttgaaa agcaataacg
     9241 agtcccccca taggaagata cctaaaaatc aagaaataaa ggactcccgg cagaatcatc
     9301 acatataaaa atttattcgt tttgattcct gcaattattt tttttcgtct tctgagcttt
     9361 tcttcttttt tcgatagcgt cagttgaaat ggttcttgtt tctttacact ttccataagc
     9421 ccctccttag ttttgtgaat gtttgctgct tatgacttca tgatagataa ctatctgaat
     9481 tacgtatata atcattttta ccgtaagtaa aaacgcttac atagcaatga tttttgtgct
     9541 atacaaaacg ataaagtgta ttttttcaca aataaactac gcatagcaaa ccttctattt
     9601 ttttctatct gacacttagc ataggaaatg attatatacg taatctttca ccgatgataa
     9661 attgaacata tagaaacaga aacatttaat ttattttgag gaggagccta tctatatgaa
     9721 aaagaaagtc tctataggag aaagaagttt tacacttttc aacaatacgt ttctaatttt
     9781 gctagctttg atctgtatcg ttcctttttt aaatatcatc gctacatctt ttgcttctac
     9841 acaagaagtc gtcgcgaaaa agtttatcct ttttcctact accttttctt tagacgcgta
     9901 tcgctacatt ctttctacgc cgaccatttt tagagcactt gctgtttcga ttggtgttac
     9961 aggtgtcgga acgatcgtca gtatgtgtgc aacttcactg atggcctacg gtctttccag
    10021 aaagtatttg tttggtcgag gtttcgtcaa ctttcttgtt gtattttcta tgttgttcag
    10081 cggggggatg attccaacgt tcttagttgt tcgttctcta gggctggtca attcttactg
    10141 ggccatgatc ctgcctgtgg ctgttaatgc aatgaatatg atcattatga gaaacttttt
    10201 ccaagccttg ccggatagtt tggaagaatc agcaaaaatg gatggttgca cagattttgg
    10261 tgtatttttc aaaattatgc tgccattagc gttaccgtct attgctacta tctcgctttt
    10321 ttatgctgtt acttactgga acacttatat gactgctatt ttgtatatca acgattcatc
    10381 taaatggccg atacagattc ttttacgcca aatcgtcatt gtatcaagtg ggatgcaagc
    10441 agaaagtagt gccgttgatg tgattccacc agcacaaaca gttaaaatgg ctgttatcgt
    10501 tattgcaaca gtgcctatgc tgattgttta tccttttgtt cagaaatatt ttgtcaaagg
    10561 agcactcgta ggttctgtca aagggtgatt atgtactgaa cgcaaaatgg tagttgtgtc
    10621 agaagtggtc agcttcaaga aataagaagg catttacgaa aattgcttct taaatttttg
    10681 tgaaatttcg gcttattttt cgaagaatct actcctggaa caccgtgtat cagaggttgt
    10741 gaaagagacg ttcagccttg agcgatgata aaaactctca caaacattgc gacgagtaac
    10801 ccacaggagc aataagaagc gattttgaaa aatagcttcc catatttcac aaaattatgc
    10861 cttattgtcg atagcctgtc tcttgaacgc catttattcg taaaaaagaa ctgtgacaag
    10921 acttttgtca cagttctttt tgcgtgctta gccttgaaat ttaactatcc ataaatacaa
    10981 atactagggt tattctaact tcttttattg cttgaatcag tccacaagaa aaacagcctc
    11041 ttgattcaca aatatttttt tcgatattct cccggcgtaa tcgattcctt ctttttaaag
    11101 aaacgaataa aattttgcgg atttcgatat tgcaatcttt ctgcaatctc ttttacggag
    11161 aggttagtct ctttcaacca tttttttgct acctctaaac gatgattttg aacaaaatca
    11221 ccaaattttt ctcctgtttc ctttttaaat acattactta aatagttagg gttataatgc
    11281 aacctatcag cgatcacatc taaagacaga tcacgatcat attctgtgta gacgatatgc
    11341 ataatattct ccgacaaatt tttaaattca tgatcagtga ttgcttgaga gcattctgtg
    11401 attggcagaa ctaattgttc taaaacaagt ttttctagtt ctttgggatg ataattgttc
    11461 agtgctttta cataaatttg tttcatgtta tcaaagtttt ttgaatctac tcccaataac
    11521 tgtcctaatt gaacgagttc attgatcaag cggaccgaaa ctacttcccg actcaaagga
    11581 tttttatttt gtgcaaacaa tgcatcaatc aattcatgta ctaaattctt cacttcctga
    11641 tttccagaac gaatggcttc aaacaatcga ttttgttctt ctatcggata tctaataagt
    11701 gttttttcat gagcagcagg aacgatttcc tgataaaaaa taattgaact tggaccagta
    11761 tttacacgat aatacagggc ttcttttgcc cgatcaaccg attgcttgct ttcattcagc
    11821 gtttcgaacc ggttactgat gcctatactg atagttaaat tcaagtattc ttttacacgt
    11881 ttttgaatga gtcggcaata ctccatgact tttttgcttg cttctgcatc atctttatcc
    11941 attcgataaa tagtggcttg catttcttca ttcaaaacga tcggcagcat tcgattttcc
    12001 tttggcacaa tttccgtgat catgttgttg atcgctaaaa gtagcaaatc acgctcccca
    12061 ccgtgactat cttctaacag atcgatttga accaaagctg tataatagca gtcattttga
    12121 gaactataac caaattgctg caaccgctga tctagttctc gcctttctac tcgatttcta
    12181 aataaactaa gtacaaatag cgtttccagc tgtggctttt gggtaactaa gtgtgcactt
    12241 aacgcttctt tttctcctat gatccgactg actgattgag cgaccaaatc tagttctttc
    12301 ccactgaatc cttttgattt aagattcaga cgttcctgta tttgagaaat cggacgggca
    12361 aatctgtccg aaaataagta ggaaagaaga ccaatgagtc ctaccagtgt taaagacact
    12421 gcaactagcc cgattctcag attgcggatc attgcgccaa ttgcactaga gtcaacttct
    12481 aatctgtaaa tccaattatt ataatctgat ttcagtacta tatacgtttt atctttttct
    12541 cttatcactt tcagctgatt gtaatctaga tccacgtccg ttaatacagt tggttgctct
    12601 ttatttttta ctgtctgagt agtaaaaagg ttgccaactt cgttttcaat agaaagtatg
    12661 tcttctccac tttcaccaac aacttgttct attgtatgtt tctctatcgt agcaattccc
    12721 aatgcatatt tttctttttc aaacattggc aaagaaatca ccatttcgat tcctttgttt
    12781 ttcttttgcc aaaataaatt atttttgtta tttatgtact tctgttgata cttttcaatt
    12841 tcctcttcgg tcagttgact caacgatcca ttgattactc cccatttacc agccaaactg
    12901 actaaatcat aattattatc atccataaca atcgtttcta taaaatttag ttctttcctg
    12961 atttcagaat aagtggagta atctgtatat gtcagcggat gcttgaatat gtccgagaaa
    13021 tttttggtcg aaccatacgt agaaaacgaa tattcaatcg ttctaatttt ccattcaacg
    13081 ttagttttaa cttgcctgag caaattcttt ttgtctgtta aataactcga ttcgatcgat
    13141 tcataggtat tctggtaaat gaataaacta aatcctatga caagtacggc tcctaataaa
    13201 aacatcggta aaaatatttt ataaaatact gtacttttct tcattttctt cctctcctcc
    13261 ttttaaaata aaaggctttc atttttctgt aaaattgata ctcatctaat taaatgagca
    13321 ttccccgctt tcaagaatag cataattaaa aataaaaatc caagtaattt ttaacgtgtg
    13381 caatcttttc atatcaaaga tctattgact tatcgtagtt atcgtaatat gatgaatgcg
    13441 ttaacaaaaa gtgaaaaagt aaggaaaatg atggaatggt aacgatcaaa gatgtcgcta
    13501 aagcagctgg cgtttctcat acgacagttt ctcgtgcttt gaataacagc agtttaataa
    13561 aaaatgaaac aaaacaaaaa atacgacaac tagctaagga aatgaattat tcgccaaact
    13621 atagtgcaaa aggtttagtg aatcaaaaaa aatatctaat cggactattt ttctcaagtt
    13681 tgcataaagg aacttcatca aatttcctag cagattccat tgcaggcatc cattcagtat
    13741 tagacacaaa ttacagttta tctgttgaaa gtatcgatgt tgttgattta acagaaatca
    13801 acctgcaacg ctacgatgga ctgatcgtta tgagtcaaag tgatgatgat cagcctttca
    13861 tcgactatct caaagaaaaa gaatttcctt tcgttgttgt caatcgtcac atcaatgatc
    13921 cagcggttat caacgtcact gccgatgatg cggccggtgt tatgcaggca gtagattacg
    13981 gtattggatg cggacatacg aagatcgctt atattggcgg aaagaaaaat tttcgttcga
    14041 caaaagaacg ggaaaaaggg gtattgaaaa gtctaaaaaa agcgagcatc cctgtcaatc
    14101 cagcctattt tttaacagga gattacagct tagaaagcgg cctccaaaac atggaatacc
    14161 tattatcact ttctgacctg cctactctgg ttttttgcgg caacgacgac atagcgattg
    14221 gtgcattgag agctgtacat ttagctggcc tccgtgttcc ccagaacatt tctctgatcg
    14281 ggttcgatga ttctccagtc gtctcctatc tcaatccgcc gcttaccacc gtacgtaagc
    14341 ctttgaaaga aatgtgtcaa gaaggaacaa agcttttact agatttgatc gaaggcagga
    14401 cgatcgaaca atctaaaatt gagctctcca ccttgttgat ccttcgtgag tcgatcaatt
    14461 acttgtaaac tacaagtaat tttatataaa aatgttaacg tgtgcataac acagaaagga
    14521 agtttatatg aaaaaactga caaaagaatt agtgaaaaaa aacgaagcac ctgtaaaagt
    14581 tcttcagttt ggtgaaggca attttatgcg tggatttatt gattggcaga ttcagcagct
    14641 gaataaccaa caattgttta aaggtaatgt tgcaatcatt caacctttag aacaaggtct
    14701 tggaaatatg atgaaccagc aagataattt atatacggtc attttacaag gactcagcaa
    14761 tggcgaaatc gttgatactt ccgagattat caccgtagta gaacaaatga tcaatcctta
    14821 tgatgaatgg gatgcttatc tatctctagc agaaaatgac gacttggcct atatcgtctc
    14881 caatacaaca gaggcaggaa tccaattcgt ggagactgat aatttaacag atactccccc
    14941 acagagcctc ccgggcaaac tgacagcctt tttataccga cgtttcaaat taaacaaagc
    15001 tggctttacc atcattcctt gcgaactgat tgacagaaac ggcgaacgtc taaaagaaat
    15061 cattttacgc tacgcagaaa aatgggattt agaacctgct tttgttgagt ggttagaaaa
    15121 agaaaatgtt ttctgttgta gtttagttga tcgcatcgtc ccaggttatc ctcgcgaaga
    15181 tgcaggtgtt ttcaatcaac ggcataacta tgaagatcaa ttgatggtca aagcagaacc
    15241 attcatgctt tgggtgatcg aagggcctaa agaattggag aatcaactac cattcaaaca
    15301 ggctggcttg aatgtcatca tcacagatga tatgactcct tatcgtgaac gtaaggtgca
    15361 tttgcttaat ggaccgcata cagcgatggt ccctcttgct ttgttggcgg aattggaaac
    15421 tgtagaagaa gtaatgaaag atcctttatt ctccgcatat gttgatcagt tattcaatct
    15481 agaattgatt cctatgcttt ctttacctaa agatgaatta gccatttatg ctgaccaaat
    15541 caaagaacgt ttcttgaatc cttttgccca tcacaaatta gaagcgattt cattaaatag
    15601 tgtttcaaag ttcagcaccc gtctgcttcc agtgttcaag aagtatatcg aagaacaaaa
    15661 tcaggtacca ccgttaatca ctgtttcact agctgcgcta cttcttatgt atcgaggcga
    15721 tcaagtaaag ccacatgatg atgaaaagac gataagcgaa tttactgatg cttggtctga
    15781 taatggaaca gctattcctc gattattgca aaacgctgcg ctttggggag aagatttgtc
    15841 tcaaataccg aatgtgacag atacagttca agaaatagct gcaaagatcg atcaaaaagg
    15901 tgtccggtca gtattacaag aaatgaaagg aacgatggtc catgcttgaa accctgcaac
    15961 caacaatgaa attaaatcga cgagacagtg tggtcgtcgc attaacccct attccaaaac
    16021 aaacacaact gacgattgat cagcaaacga tcaccactct tgaagatatc ccgcaaggtc
    16081 ataagattgc attgggtgat ttgaaagctg gagacaacgt gatcaaatat ggctatccaa
    16141 tcggtcatgt gacgacagac gtaactgccg gtcaatggct gcacactcat aatgtaaaaa
    16201 ccaatctttc tggtgaattg gattatcgat atgaaaaaga tgtccatcca agtcattacc
    16261 tttttgagaa ccgaacattt caaggttact tgcgaaaaaa tggaaaagta gggattcgta
    16321 atgatttgtt tttagtacct accgttgggt gtgtgaatgg cattgctgaa ctgatcgtca
    16381 aacagttcaa ggaaaaacat ccagatctgg gacagtttga ccatattacg attttaaagc
    16441 atccttatgg ctgttcgcag cttggaaaag atcatcaaaa tactcgagaa attttagcgg
    16501 acgcagtaaa tcatcccaat gccggaggag ttttagtatt tggattaggt tgtgagaaca
    16561 atacagttcc tgaattcaaa aaaattttag gtgaatatga tgaagaacga gtgaaatttc
    16621 tagtggctca agatgtttac gatgagatcg aacaaggtgt ggctctatta gaagagcttt
    16681 taacagcggc ggaaaatgac cagcgcatat cagtcccgct ctctaagttg aatgtcggtt
    16741 tgaaatgcgg cggttctgat ggtctatcgg gaataactgc taatccttta cttggtgcat
    16801 tttctgacta cttgattgct caaggaggca gcacgatctt aacagaagtg ccagaaatgt
    16861 ttggcgcaga acaagtattg atggcacgag cggaaaatga agaagttttt gactcaatcg
    16921 ttgacttgat caatgacttc aaacagtatt tcttgtctta cggggaacca gtttacgaaa
    16981 acccatctcc tggtaataaa gcaggtggga tcactacatt agaagataag tctttaggct
    17041 gcacgcaaaa atcaggaagc tctcctgtcg ttgatgtgct acaatatggc gaaaaaatca
    17101 gaaaaccagg gcttagttta ttgcaagctc ccgggaatga tcttgtagct gcctctgctc
    17161 ttgcttcatc cgattgccag ttggtcttgt ttaccacagg aagagggact ccgtttggct
    17221 catatgtccc aacggtcaag gtatctacga atacaactct ttttgaacgc aaaggtcatt
    17281 ggatggattt taatgcaggc gtcttattag aacaaccaat ggaagtaatc ttagaatcgt
    17341 ttgttcaaaa aatcatcgct gtagctagtg gagaagaaac gaaaaatgaa caaaatgacg
    17401 ttcgagaaat tgccattttt aaaaacggag taacactttg attttcataa aacagtataa
    17461 aaagaaagaa ggaagattta tgtttttatc tgatgatttt ttattaaaag acgattgggc
    17521 gaaaaagctt taccattcct acgcaaaaaa catgcccatt attgattatc actgtcactt
    17581 agatccaaaa gaaatttacg aaaacaacaa tttcagcaat ctgacagaag cttggttagg
    17641 aggcgatcat tacaaatggc gcttgatgcg agcttgcggc gtgcctgaag aaaaaatcac
    17701 aggaaatgct tctgattttg aaaaattttt agcttggtgt caaaccgtac caaaaattat
    17761 cggaaatccg ctttacagtt ggtcacatct tgaattgaag cgtttcttcg gtattgactt
    17821 gttgataaat gaagaaaatg cagagaaaat ttggacacaa gcaaatgaaa ttttggcagc
    17881 tcctgatttt cgccgtcggg aaatcgcaaa aaaatcgaat gtagaagtga tttgtacaac
    17941 agatgatccc gcagacgact tgcactatca tcagttgctt gctcatgaag aaccagattt
    18001 gaaggttcta ccttcattcc gtccagataa agcactcaat attgaaaaag aaggatttaa
    18061 tgcatgggtc acactactgg aagaagcagc tgaccaaacg atcaccacct atcaagaact
    18121 gatagaagtc ttagggcagc ggatagaata cttccatcaa catggatgtc gtgtttccga
    18181 ccacgcttta gacatcttac gctataaaga agcggatgaa acagtattag aagagatttt
    18241 tcaaaaagca cgagctaaca aactattatc ttcagatgaa atcgatgctt atcgtacaga
    18301 aacactgata catttgaccc acttctatca ttctcataat tggacgatgc agctgcacat
    18361 ccatgcttat cgtaactgta atacacaaat gttggaaaaa ttagggcccg ataccggcta
    18421 tgatggaatg aatgatcttt ctttgactat tcctctgcaa aaattattga accgtgcaga
    18481 agaaacggat cagttgccaa agacgatcct ttattcattg aatccgaatg actatcctgc
    18541 gattttagct ttgatgggct gtttccaaaa agaacagaat ggaaaactgc agttaggttc
    18601 tggatggtgg tacaacgata ctcgagctgg tatgcgcgat caattgacac aatttgctga
    18661 tggtagtgct ttggggaatt ttgtcggcat gctgacagat tcaagaagtt tcctgtccta
    18721 cactagacat gaatatttcc gtcgtgttct ttgtgaattt ttaggcgaaa tggtcatgcg
    18781 aggagaagca cctgaagatg aacaactttt aggaagtcta gttcaagcga ttagttatac
    18841 aaatgctaaa aattactttg gcttttttgc ctaattgatt aatagtaaca gtaacgaatc
    18901 aaaaacagga tagtgaaata acggaatccg tttatccacg ttttagagaa gaagacggac
    18961 caaatttgct ccgtcttctt ctcttgctgt tctgcttcat tgcctataaa cctttatgaa
    19021 agaagagata acatgcctac tatccatatt gccggtgatt ctactgcggc agaaaaagat
    19081 gaaaacaagc gtcccgagac aggttgggga gaaaaaatct ctgcttattt ttcacccgag
    19141 atccagatca ataaccaagc aaagaatgga cgcagcacca aatcatttat ccaggaagga
    19201 aggttagccc gtctagcttc acagtttcag ccgaatgatt tcttatttgt acaatttgga
    19261 cataacgacc agaaaatcca agaagaaaga ggtacgcttc ccttcggtag ctatttaaaa
    19321 aatctgcaga tattttttga atgcgcaaaa aaagcgaacg tccaaatggt cttactgaca
    19381 tctgtgacgc gacgagatta cttagaaaac ggaacattga atccagatat acttggagac
    19441 tatccaaaag cgatgcgtgc atttgctgag aaacaccata ttcctctttt agatgtgttc
    19501 tcaagatcac aagaattgtt ccagaccttc tcaaaagaag agacaaaaaa attctacctg
    19561 catcttatac ctgatacttg taaaaattac ccagaaggac taaaagacaa tacccacttt
    19621 agtccagaag gcgctgataa agttgcccga ataattgttg aattgataaa agaatcccgt
    19681 ctgcctcttg ctaattattt acaaaaagga gtttgagatg tttccattaa atttaggcat
    19741 ccgtgcccac gatttagatg ttcgaaatcg tgaagaaatc gttcaaaaag tatcacagct
    19801 tcacttgtcc catatccaat ttgcaccaca aaaagcattt cctgaacaac ttaaattaac
    19861 tgacatgaca cttgggagtg ctgcctattt tggtgaatac ttccaaaaaa acaatatcga
    19921 actttccatt ttaggatgct atatcaacct atccagcaaa aatcaagaag ttcgtgcaca
    19981 agcattggca actttcaaac agcaaattgc tctgtgctct gcctatcaag ccaagatgat
    20041 tgctacggaa accggtagtg tctctgtcgg ttacacaaaa gaaaatttta ctgaagaagc
    20101 ttatcagatc gccagaaatt ctatcattga attagtcgct catgcagaaa attttggcgt
    20161 aactgttgct gttgaagcag ggatcaatca tccaatctat aactatcagt tagctaaacg
    20221 tttaatcgat gaagtagctt caccaaattt aaaaatcatt ttagattgtg cgaatttgat
    20281 gcataaagaa aatcatgctg atcaggaaaa agtgatccga ggtgccttgg acaatttaca
    20341 tggttatatt acagcgcttc atctcaaaga ctatatcatg gaaaatggaa agatccgcat
    20401 ggtacctgtt ggcaagggct gtttagattt ccgcccggta ctgcattata tcaaatatga
    20461 gcgtcctcta atgtacgcca ctcttgaagc tactcctgag atttatgttc ctgaagcaat
    20521 agaacacttg cagtttattt accaagattg ttaaggagaa gattatggag gaacgaatac
    20581 agaaaagtta tcaaaaatat catgcaaatg cttttcagac attgctgcat ctttatcagg
    20641 gaaattattg gcgttttgcc atgtcaggac tcttttttat tatcaaacat cttccatctt
    20701 ggttaatgcc gatcgctatt gccaatgtaa tcaatgcagc ttcatcgaaa gattcttctc
    20761 agttgcaggc catttatttc aacgcttttt tgatggcttg tctgattggt caaaatatat
    20821 tgacgaatta ttttcatgta aaataccata gcacttcgat ccgtcaagta gaagctggac
    20881 taagagcgac ccttatcaag aaaatccaag aactatctat tccttatcaa aaagaattgc
    20941 aatctggacg aatccagtca aagatcatcc gtgatgtaga agctatccaa acgttgtcct
    21001 ctcaagtatt tgtcagcgga ttaaatatcg ctgtcaattt attcgtagct ttaggtatca
    21061 cgctttataa aagtcctgtt gttttcatgt ttttcttatt gactgttcct ctcgccgcat
    21121 tcgttactct ctttttccgt aaaaggatca gacagtccaa ctcagacttc aggcaagaaa
    21181 tggaagagac ttctgcaaaa gtgatcgaag tgatcgaatt agcaccgatt gctcgagctc
    21241 atgggttaga aaaacacgaa acaactcgct taaataagca ttttactcaa atctatgaaa
    21301 aaggattcca cttagatatc ctgcaatctg ttttcggatc agtcagctgg gctagttttc
    21361 agatatttca gctgatttgt ttgattttca ctgggatatt agctttacaa gggcgcatcc
    21421 aacccggcga tgtcgtgatg tatcagacgt atttcacgac gatcgtcaat tccgtttctg
    21481 gaatgatcac gctgattcct acgatttcaa aaggcatgga atctatcact tctattggcg
    21541 aactgttgat tgctggcgat gtagaaactt atcacggtaa gaaaaaaatc caacatttgg
    21601 atggaaattt tcgttttgag gatgtttctt ttctttatcc ggactcactc aaaccgatta
    21661 tcgatcattt ttctctagag gtgaaagctg gagaaacaat tgcttttgtt gggccatctg
    21721 gctctggaaa atctacactc cttaacttat tgattggatt tatccagccg acttcaggaa
    21781 aaatttttct agatgatcag cctttggatg aactggatat gcgaagtgtg cgaaattatt
    21841 tagccgttgt tcctcaaacc actttgttgt tctccgcttc gatcaaagag aacatcactt
    21901 atggcttgaa aaacgtatca aaagaacgtt tagatgaagt catcgaagct gcgcaacttt
    21961 cttcactgat tgaccagctt cctgatggtc tggatacctt agtaggcgaa catggcaaca
    22021 agctgtctgg tggccaaaag caacgtatct ccattgcacg agcgttgata cgtcagccaa
    22081 aaatcatttt gctagatgaa gcaacctcag ctttagataa ccagtcagag aaaaaaatcc
    22141 agcaagccct gaattacctg acagaaacac ccactacttt tattgtggct catcggcttt
    22201 caacgatcaa agaggcagat aaaatcgttg tgatcgatga aggaaaaatc gttgaaatag
    22261 gtacttacga aaaactgtta gctcaaaaag gctatttcta ccgcttgaaa aatgaatgac
    22321 aaaaaatagg agctatgaca agacttttgt cgtagctcct atttctgaat aaacggtgtt
    22381 ctcctgccaa ctcctcggtc acaagtcaca atagtcatca aacatgagaa gctgtttttt
    22441 gaaattgttc cttgtaactc agagttaaac ggtaggctca cgacctcttg gtaaatggtg
    22501 ttcaagagac agcctatcgg caataagaag caattttgtc atcgctcaag gccgaacgtc
    22561 tctttcacac cctctgttgt ttcataaaat agttttattc gctgtaaagg tcgcgaactg
    22621 gtgtcatgat gatacggttc aagtcattga tgattgtact gaagccttgc tctttttgca
    22681 tcaagtcatt gattacttct tctgattgga ttttcacagc catttcttgt gctttcattg
    22741 catcttcgtc gctgaactct tgtccactca tttgtttttc ttgtaattcg atttgcatcg
    22801 cttggaagtc tttgaacagt ttgtgtgctt cttcgtttgc ttgtactttc tcgtacgctt
    22861 ctttcaacgc tttgaattct ggtaattcac ggatctcacg ttcgatttga ttcgcgctat
    22921 cgtagatgtt tgacatgtta tgccctcttt tcttttagta gttactctct attgtaacat
    22981 ggggttattc gttttcaaag gggctactct ccttgatatt tccgccagag gtctttagcc
    23041 gcttcaccaa ttccctgaac gccttctttc accatatcgc cgaagttttt cagcccttct
    23101 tttgcttgtt caccatattg attcaagtta tcttgccaat ttccagactc tccattagaa
    23161 tttcctgtgt ttccaacttg atctgcagct accacttgtc ctcctgtggc ataggcatct
    23221 gctacactaa atcctgtttt tgaagtatac ggaaggatgc cttctgccgc tgatttgaag
    23281 actttcccga caacatttcc acttgtccct tccaaatagt gcagcttgct ggattcttcg
    23341 taacccatcc atgtcgaaat cactacgtct ggcgtgtaac cgatcatcca ttggtcattt
    23401 gcttttgtgg catcgaaatt agtttctgtg gttcctgttt tccctgctat cgtataacct
    23461 gcaggattcg cagcagccgc tgttccattt gaaaatgtac caagaagcat gctggtcatt
    23521 tcatcggctg tttcctttga tatgacctgt tttggttttt tgtttgtgtt atcgacgatc
    23581 accgctccag tcgaatcaac gattttcgtg ataagatgcg gcgtatacat ctgaccatca
    23641 ttggcaaatg cactgtaagc acccgccatg atcaatggag atactccttt ttccagtcca
    23701 ccaagggcta agccataata cttatcagat tttgctaaag gcaggccgaa ttcttctgct
    23761 ttatcatatc ctttttgtaa accgatctca tgcaaaagcc atacagtagg caaattcaaa
    23821 ctttgtgcaa cagcttgata catcggtact tctccttggt atgtcccgtc aaaattatgc
    23881 gcatcgtaat aactttgcgg ttcgtctttc aagatactgg aaggcttgta tccagcttcc
    23941 agtgctggtg tatatacgct taatggtttg atcgttgatc ctggtgaacg acgcatctgt
    24001 gtagcaaagc tgaatcctct aaagacatga tctcctcttc gtcctactag tgcttgtaca
    24061 ccacctgtat tcggatctaa agctactgaa gcagattgga ccatggcacc atctgaagca
    24121 tttgctggga ataatgcact gttttgataa gtagcttcta attgcttttg ataatcctga
    24181 tctaaagctg tatagatttt atagccttta ttcatgacat cttcttcatc aagaccatac
    24241 tcgttaactg cttcatcgat cactgcatca aaataataag gataacgata accagaatct
    24301 gaattatcgt atgtgtcatt aagatacgcg ctgatatctg tctggctttc tgcttgttct
    24361 tgttcttttg tgatttttcc gttttcttcc attacactta gtactgtgtt tttccgatca
    24421 ttcgcatttt ttgggtgatc gatcggatta taaataccag gacctttcag cattcctgcc
    24481 aaagtagcag cctcagacag cgtaacattc gttgcatcta ctccaaaata ctttctggaa
    24541 gcgtcttgga caccccatac cccgttgcca aaataggcat tgttaagata catggtcaag
    24601 atttcttctt tgctgtactt cttttcgatc tcgattgcca aaaataattc cttcgctttt
    24661 cgatcaaacg tttggtctaa tgtcagatac gcatttttgg caagctgctg agtgatcgta
    24721 cttcctccgc cccctgatgt tccgccagac aagaccattc ggactgctgc acgggcaatc
    24781 cctttgatat caaacccgtg atgctcgtaa aaattcctat cttctgtcga gattactgca
    24841 tcttggatat agggagaaat cgcgtttaat tctacatatg ttcctttttg ggcgtacagt
    24901 gtgccagctt cttcgttttt cttatcataa atgactgtcg attggcttaa tcctgatttt
    24961 aacgtttcga catttgcttg ttttgctaaa ataaagaggt agatacttgt cactaagaca
    25021 cagatcaaac caatcagcaa aatcaatttg ttcacatgat attttttcca gatgcgcttt
    25081 cgccatcgat ggaattgtcc gagatatggt ctgatccaac gccaaaaact gatcaaagca
    25141 gcttttatct tttctaaaat gctttgaaaa tccatttgct tcttctcctg tctatttgat
    25201 tcaatcgtct tattttacca tagtgaaaga gtgttttctt cctgcttaag ttgtagttca
    25261 atatgttttt ttacgttagt gcaagtagag taacataaga atgaacagaa aaatgtaagt
    25321 tttttcttaa atatgtttta aaaatggtac aattagtctg cgattgcact gaaaggagta
    25381 cacttgtgaa aatcacgatc actttaccta tgaaccatcc tgaaacaaca attcgcgact
    25441 tgcttgaaaa ggaatggtta gttcctagaa aggttcgaca ctttttgcgt atacgtaaaa
    25501 acgtttggat caatcaacaa cctgctttgt tccatcatga agtaaagggc aatgaccaga
    25561 tcacattgtt attcgaagaa acagattata attatcaaga agttcaatta ggagatgcga
    25621 agcacatccg tgttttatat gaagatgagc atttgattat agtggataag cctgcaggaa
    25681 tgaagactca tccaaatgaa ccgacagaaa acagtacctt actcaatcat ttagctgctt
    25741 atctcgacac taaacagcaa cgaccttacg ttgtccaccg cttggacaaa gaaacaagcg
    25801 gtgccgtttt atttgcaaaa aatccatttg tcttgccgat acttggaaga atgttggaac
    25861 aaaaaatgat ctatcggaga tatcaagcca tcgtatgggg agaaattgcc aaagatatga
    25921 ccatcaccaa taaaattgga cgagatcgcc atgatcggag aaaaagggtc gtagatgata
    25981 aaaatggtca gagcgccttg acacatgtca ctgtcaacaa agtactttct gaacaaacac
    26041 aagtctattg tgttttggaa accggacgta cacaccagat cagagttcat ttagctcata
    26101 agggacatcc gatcgttgga gaccctctct accaaaatag aacagctgaa cgactgatgc
    26161 tccatgcatt agaactgcac atgatccatc catttactca agaaaaaata gtcggtaaag
    26221 ctttacctgg attatgggaa tagaaataaa aaagaacaga gtctt
//
LOCUS       DAXWOO010000022        25881 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.022, whole genome shotgun sequence.
ACCESSION   DAXWOO010000022 DAXWOO010000000
VERSION     DAXWOO010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25881
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.022"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(19..729)
                     /locus_tag="ACGE70_000748"
     CDS             complement(19..729)
                     /locus_tag="ACGE70_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFF8692403.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(726..2198)
                     /locus_tag="ACGE70_000749"
     CDS             complement(726..2198)
                     /locus_tag="ACGE70_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFF8692404.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            2336..2809
                     /locus_tag="ACGE70_000750"
     CDS             2336..2809
                     /locus_tag="ACGE70_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFF8692405.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(2962..3513)
                     /locus_tag="ACGE70_000751"
     CDS             complement(2962..3513)
                     /locus_tag="ACGE70_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFF8692406.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            3618..4148
                     /locus_tag="ACGE70_000752"
     CDS             3618..4148
                     /locus_tag="ACGE70_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFF8692407.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(4631..5074)
                     /locus_tag="ACGE70_000753"
     CDS             complement(4631..5074)
                     /locus_tag="ACGE70_000753"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8692408.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(5090..6250)
                     /locus_tag="ACGE70_000754"
     CDS             complement(5090..6250)
                     /locus_tag="ACGE70_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFF8692409.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            6757..8457
                     /locus_tag="ACGE70_000755"
     CDS             6757..8457
                     /locus_tag="ACGE70_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692410.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLHA
                     "
     gene            complement(8559..9392)
                     /locus_tag="ACGE70_000756"
     CDS             complement(8559..9392)
                     /locus_tag="ACGE70_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFF8692411.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(9696..11861)
                     /locus_tag="ACGE70_000757"
     CDS             complement(9696..11861)
                     /locus_tag="ACGE70_000757"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFF8692412.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            12121..14415
                     /locus_tag="ACGE70_000758"
     CDS             12121..14415
                     /locus_tag="ACGE70_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFF8692413.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            14408..15073
                     /gene="pgmB"
                     /locus_tag="ACGE70_000759"
     CDS             14408..15073
                     /gene="pgmB"
                     /locus_tag="ACGE70_000759"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFF8692414.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            15066..16073
                     /locus_tag="ACGE70_000760"
     CDS             15066..16073
                     /locus_tag="ACGE70_000760"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFF8692415.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            16095..17114
                     /locus_tag="ACGE70_000761"
     CDS             16095..17114
                     /locus_tag="ACGE70_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8692416.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(17395..18762)
                     /gene="nhaC"
                     /locus_tag="ACGE70_000762"
     CDS             complement(17395..18762)
                     /gene="nhaC"
                     /locus_tag="ACGE70_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFF8692417.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(18871..20292)
                     /gene="tyrP"
                     /locus_tag="ACGE70_000763"
     CDS             complement(18871..20292)
                     /gene="tyrP"
                     /locus_tag="ACGE70_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFF8692418.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(20535..22412)
                     /gene="tdc"
                     /locus_tag="ACGE70_000764"
     CDS             complement(20535..22412)
                     /gene="tdc"
                     /locus_tag="ACGE70_000764"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFF8692419.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(22703..23959)
                     /gene="tyrS"
                     /locus_tag="ACGE70_000765"
     CDS             complement(22703..23959)
                     /gene="tyrS"
                     /locus_tag="ACGE70_000765"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFF8692420.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(24071..24276)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            24539..25840
                     /locus_tag="ACGE70_000766"
     CDS             24539..25840
                     /locus_tag="ACGE70_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFF8692421.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
BASE COUNT         7888 a         5323 c         4661 g         8009 t
ORIGIN      
        1 tacatggtgt tcaaaatatt attcgccttt tgcttctttc actaattttg cgaatgcttg
       61 ttgttggaat tcagcagcta aggctttcgt acgttgttct tgctctgctt tgctcatttt
      121 tggattttca gtcattgctt ttaccatacc gtcttgaaca taagcttttg cttcactttc
      181 catcgtactt tgctgtttct tcactaattc agctaacttg gctgcttggt ccgtacttaa
      241 ttctacccat gtctctggga ttttgaatgt attcactcgg ctgtcatatt cgtgattgtt
      301 atcagcaata ttgaaccaga atttatacat gtcgtttttg tattcccaat aagtagtttt
      361 cgtcactttt tcagcatcgc cgtctgtctt ttctacttta tttgtttcgt tatccgtacc
      421 agttgctttt ggtttttctt gtttttcatt tgttcggtaa agatacactt tttcttttcc
      481 attccctaaa gctttataca aaagcatatc tgctccttgg gaatcagcac tggaaacaat
      541 tttttctgtt tttgtttccg ttactttttc cattccaaag tgattggaca aattagcgac
      601 gatcaaactt agtgaagcga cgaagattag tccgaaaatc aaagataaaa aggtttgcca
      661 tgttttttta gcgaacacgt tcgttaaagc aaaagcaagc aagctgacaa tcaaaactcc
      721 aataatcatg cattatcccc cgctttcatt agaacaggtt cttttttctt caataaaaag
      781 gcaataataa agccaagaat cccaaagatg atcgctacga taaacgcggc atggtaacct
      841 gacagtgtgg cattgattgc ctgatcttta taagctaatg gcgtattatc caatacagat
      901 ttgcttggga tatttgcttt tgtcacattc gtcaacacac tgatcaaaat agctgtacca
      961 atcgagcttg cgatttgacg gaatgtatta tttaccgctg tcccatgact gatcaagtta
     1021 tttggcaatg cattcatacc agaagtcgtt accggcatca tgaccatgga gatcccaaac
     1081 atacggatcg cataaaggat cacgatatat aaaactggag tcgtttttgt caagaaagca
     1141 aatggtgcag ttgccgctgt caaaatgaac atccctgtga tggctaaacg tttcgcgccg
     1201 tatttatcga aaatcgcacc cgtcacaggc atcatagccc ccatgatcaa tgcacccggc
     1261 aacaacatca agccagaatg gaatgctgat tctccacgaa tattttgaat atacaatggt
     1321 acgaccattt ctgcccctac catcgccata tttgttacac cagctaaaac agctgcaatc
     1381 gtgaatgtag atgatttgaa tacacgaagt tccaagaatg gatgttccaa atgtaattga
     1441 cgccatgaga acaaggcgat gaaaatcaca ccaacaacta agaaaccaat tactttcaag
     1501 cttccccagc cgtcatctcc tacactagaa aatccgtaaa gtaaactacc gaatccaatc
     1561 gttgataaaa tagcggataa aatatcaatg ctaggatttg acaatggaat aacacttttc
     1621 attaggaaga atgctaaaac caagacaaga acaacgattg gcagtaccat accaaacaag
     1681 tcacgccaat ggtagttatc aatgatataa ccagataatg tagggcctaa agcaggagca
     1741 agtccaataa cgatccctga catccccatt gctgatcctc ttttttctgg cgggaaaata
     1801 gatagcatga tattttgcaa taatggcatc gatactccca caccgcaagc ttggatcaaa
     1861 cgaccgatta gtaatgtact aaaattaggt gctgtaaagc aagtaatcgt tccgatcaaa
     1921 aagatcgtca tggcagaaag atataaattt cttgaactga atttattgat cagccatgct
     1981 gttattggga tcatgatccc gttgactagc aagaaaccag tcgttaacca ttgcacatca
     2041 gacgctgaaa tatcgaaagc tttcatcaat gtaggaaaag ctgtcgtcaa taaggtctga
     2101 ttcaaaacgg tacaaaatgt accaattaat aagacaacaa ctaaaagtgt ccgattatat
     2161 ggttttccgt aaatatctac gggttggttg ttactcatat ttctccttct ttctgaattt
     2221 tttctgaaac tactagaata ctttagtcct aaattcgcta tttgtcaacc tacttgacac
     2281 ataagttaat acggatataa ttaagggaag caagaggaca aaaggagtga caaaaatggt
     2341 tggggcttcc cttaaacgtt tgttggaaaa tgatcaattg gttgttggca tcagtgaatt
     2401 aagtgatatc gtaggtgtgt ctccacgtca attaaggtat tgggaacaaa agggattcat
     2461 tcgttcgatc gaagctgatg cgaattgtcc tagaaaatat cgtttaccta cagtcataaa
     2521 agtagaactg atcaaaaaat atttagatga cggatttact ttgtcaaaag ctgcagaaaa
     2581 ggcagaaatc cgccaaaaga aaatgcacca tgtccgtaaa gttttttcaa aagcaatcaa
     2641 agatatcgaa ttgatcgacg aacgctatac gattttacaa atcggtaact tcaaagaaga
     2701 gaatgctaag atgtacatca ttcatgacaa cgagacagac gaattaactt acaagctcct
     2761 tcctgtcgat gaaaaagctg actacgaaca gttgttaaaa gaactctaaa cgccactaat
     2821 ctcctaagta ccaagctttt taataagtag atgctacata aatacttgaa aaaaggcctg
     2881 tttgaacata agaatatccg aacggtgatc tgcgtttctt tcatatagaa aggaattctc
     2941 catcactgtt cggatatttt tttatttttt tcggttactt tcttctaata catctgttag
     3001 ccaatttgca tattctgttt cccgattgat cgcatgcgct aaaatcaggt agtgtccgta
     3061 attttcatgt ttttcttttt cagtaggaaa gatcgttttt tttcgctcta atagatgaag
     3121 tcgtttttct tcatgaaggt cacgctgctg ctgaataatt tctactaatg ccggatcatt
     3181 cacgtctttg acgaaataca atttcagaac aaattcgtct cgattaaccg gtagttcatt
     3241 ggtgggtgta tgaatccact ctgccaattg ttccttccct tcttctgtta attcataaac
     3301 tttcttttcc aatttttcac ctgtgatctc tacttgatgc tgaatgattc cttgctcttc
     3361 catttttgca agttcaggat aaatctgact atgttttgct tgccaaaatt ctcctatttc
     3421 atttttgaac gattgattca attcatatcc tgtcatcttc ttcttactta ataaccctaa
     3481 caaaatatat cgaagtgtat tttttcttgc catcagtttt ccctccttga caaatcttta
     3541 aatccattct atcataaatg agtaatcgaa aacatgtagt ttattacatg ttttaattta
     3601 taaggagatg cttaaacatg aaagaattta aagaattgga agattttatt ggaactcact
     3661 ttatctatac gtatgacaat ggttgggaat atgaatggta tgccaaaaat gaccatactg
     3721 ttgattaccg tatccatggc ggaatggtca aaggccgttg ggtaaaagac caagaagctg
     3781 atatcgttaa attgacggac ggcgtattca aaatcacttg gacagagcca acagggacag
     3841 acgtggcatt ggactttatg ccaaatgaaa agaaacttca cggaatgatc tttttcccta
     3901 aatgggttga agaacatcca gaaatcactg ttactttcca aaacgaacat atcgcagaaa
     3961 tggaagcagc tcgtgaaaaa tatgaaactt atccaaaatt acttgttcct gaatttgcta
     4021 cgatcactta tatgggtgat gcaggtatag acaatgaaga tgttattagc gaagaaccat
     4081 acgatggaat gccagatgat atccgtagcg gaaaatactt cgatgaaaat tataaacgag
     4141 taaaataagg ttgtgaaaga gaggttgtga aatcagcgtt ctgacctgag tattagagaa
     4201 agaaacggaa aaatagttcc tcatgttttg ccgttttttg agctaatacc gagggtcagc
     4261 tgattgaaca ccgtttatca agaggttgtg aaatcagcgt tatgctttga gtaataagaa
     4321 cgctagttag aaaatagctt ttcatatttt ctaggctagt gggcttatta ccgagaagca
     4381 gccgtttgaa caccgtttat caagaggttg tgaaatcagc gttttgacct gagtattaga
     4441 gaaacaaatg gaaaaatagt tcctcatatt ttgccgtttt ttgagctaat accgagggtc
     4501 agctgattga acaccgttta tcaaagattg taacaactgc atattcacac agttaaatta
     4561 gtgacttcaa aatgaaataa tgctcccagc aaaaaattag gctgggagca ttatttcgtt
     4621 attctgttca tcaaaaatca gcgttttgat tccatttcat cacaagttcc tcctcattta
     4681 ctgcttcatc tttttgctgt gtttgaattg taaaaccttg tgaaagatag aaatgaatag
     4741 ctgattgatt tttttgatag actgtcaaag tcagtgtttt attggtttct tttatttttt
     4801 tcattagttg cgtaccgatt ctttgttgcc ggtattccct tttcacaaag attcctgcga
     4861 taaactgatc aatcacccca gcaaaaccaa taatttcttg atctatttct gctaagtaaa
     4921 tcactgcatc tggtaaagct ttcctaacag attctacttg attctcccaa taagttgatg
     4981 gaatgaattc atgagaatcc aagttagcat gcagccaaat cgatagaaca gattccagtt
     5041 ctttggcaga caattttgtt gttgtgcgga tcattttttc tctccttact tattcattga
     5101 ttttaaaata aaaaccattt ttttgaagct cttttacaag ttttcgcccc tcaaaaaagt
     5161 atatcttctc ttgttttact tttctaatac caaaagaaac agcaacttta ggatcatcag
     5221 ctgaaacgat tcgataagtt ccaccaggca attgatccag cttgactttc ccatgatggt
     5281 cgctttgtac tgaaaacggc acaccatttc tagtaagtgt ttttttctta tgtttcaaaa
     5341 tgaatgaact accatttacc ggtttccctg acgaatcact aataataaat gaaaaatcaa
     5401 gcactttttt acttaatcta tactgataga ttagtcgtcc gataaacact aacagtatga
     5461 ctacagctat tatcgttcct gcaagaataa accattgccg catggttctt tcctgatttc
     5521 cttttccttc ttcttttttt accgattcgt tataaggaat ccgatgtcct gtcactagca
     5581 aacgatgcgt gttgatcata tacggcgtac atgtaagcaa ggtaactaag tcttctcctt
     5641 ctgaaatcgt tagaacagac gtgtcatctg gcagtaccac ttgtatattt ttaacttgat
     5701 aagccaattt ctcccccaaa acagtcaaaa taaacgtgtc tccgttttct aaacgatcta
     5761 attcggtaaa taaccgtcga ttaggtaaac cgctgtgagc agagatcact gaatgcgtcg
     5821 aatttccacc aagcgggaaa gaggttcctt gcaagacgcc tgctccattt tcaaggatcc
     5881 taccattgag ccgatcataa agtgaaatat ttaattggat cttaggtata ttgatacttc
     5941 cgatcagcca ttcctctaat tggctggatg ttgtactttc atttctgttc ttttcattga
     6001 aaggatcagt accaggattg aatccttggt cagttaatcg ttggtttttc tcctccattt
     6061 tctttcgctg cacttcaatt tcagctgcgg tccgtttttg tacttgttcg atacgttttt
     6121 gatcaataaa actatttaaa gaatccacat aaaatggata taaagcgatc agtaacccta
     6181 cgacgataca tattaataaa aacaaacgaa acaaaagtcg acgagactgt ttttgcttac
     6241 tctgcttcac atgtgtcctc ccttgcaagt ttttttgttc tttcttttat tatatgggtt
     6301 caggaaaaaa atagaaaatg aaagccgatg aggttgtgaa aaagctgtcc tgcatcaagt
     6361 aataagtaca gcccaaaaaa atagctactc atattttttg ttgggttggg cttattaccg
     6421 cagatgcaag cttttgaaca ccgtttaagg aagaggttgt gaaaactgcg ttatgctttg
     6481 agtaataaga gcgctagtca gaaaatagct tctcatattt tctaggctag tgagcttatt
     6541 accgagaagc agctgattga acaccgttta ccgagaggtc atgaaaaagc tactttgttt
     6601 cgagtattaa gaaaaattct gtttcaaaac aaggagatgc aacaaaactt atgtcgcatc
     6661 ctctctcctt agaactttca cttatgtttt tcttgtaaaa ttcataaata ttttcatgaa
     6721 ttttctaatt tcttgcctac ctctcgcata ggatatatga tgaatttatc taaagtttgc
     6781 aaaggagttc gacaattgaa aaaatatggc ccatttttag caattttttt cattgctttg
     6841 gcatttctca tgcctcattt attcacacat aaactaattt tgggtgctga tgccccattc
     6901 cattacaatc ggttctatga tgccgcgcaa caaattaaat atggggattt ccattatttt
     6961 ccttcccttt atggattcca gcaaagtggc aggatcgtga atgccgttta cggcccattt
     7021 ttcgcttatt tacaaggatt gatcgtgttg cttgcaggta gctggtttca ttatcagatt
     7081 ctttcaaatt tcattcttta tcttatttca ggattttcta tgctgatttt actgagatat
     7141 gtcaatgttc gaaattgggt aagtgtacca gtagccgttt tattcatgag tacgtattct
     7201 attcaatact ggtctatcaa ccaaggattc actagctggg ggacttgttt ttatccttta
     7261 tgtatgatcc ctattgctga tttcgtcctt cataaaaagt tccctgttat caaagtcgct
     7321 gtctcagtgg gacttatgac acagatccat ttactgtctt cgatcatgtt agctttgatg
     7381 tatatccctt tttatcttta ttactttttt aaccagactg aaaaaggaaa agcactgctt
     7441 caactgatta agtcggtctt gctttatttt gttctgacag caaatatttg ggcaggatta
     7501 gctattgttt acagtgggaa taagattctt cctccctttg ttaacagaga aatgagtcaa
     7561 aaggcgatag atctggatgg cagttattgg ctccattacc ctaaggcctt tcctatattg
     7621 atattatcag gttttttgct ttttattttc tttcaacaaa agaacacctt attccaaaaa
     7681 atcgtgttgt acacaagtct tgttttcttt tttctttcta ctagcttgat tccatggacg
     7741 acattgatcg aaaaaaatgt gccttttatt agtttgatcc agtttccatt tcgatttttc
     7801 gtgccattta cgctattgct tctactttac cttgcccttt cactgaacca gtggtctgaa
     7861 caaaagtggt tccgtctttt aagtatattt ggcattctta ttttttcagc acagacgatc
     7921 cagaacttat accagcatct ggaaaagtgg gaaaatgaaa catttgtcag tcgacatact
     7981 tatctattcg atacgccgga agaagcaagg aaaacattct tcagcaaaga tatgtctacc
     8041 agcttgttcg ttttccaaaa aaccacacct gactatctgc caatctataa ggagacaaaa
     8101 gacaataaat acgaccggta taacgaagaa atactagaaa acgaaacaac ttttataaaa
     8161 acacatccag gcggtctttt gacaatcaag tggaaaaaca ctggggacaa aactgttcat
     8221 atccctgtta tcgtctacga tcggactgtg ctacagcaaa atggaaaaac attgactgat
     8281 tatgaagtaa cagatatcgg gaccccaatc gtgaagcagc aaaaaggaat caacgagctc
     8341 acgcttcatt atcaaacgcc aatctatttc tatttcattc tttctcttac tttgattggc
     8401 tggtttacct tgctatgttt atttatttat catagataca agcttttaca tgcttaggca
     8461 aaagggataa atacatccga tcaaaaaact ctccaaacgg tataatcgtt tggagagttt
     8521 tattttaatc agttttttta tgttatatgt tatatttttt attcgttcac atctaattcg
     8581 atttccaccc cgtagtgatc actgatgatt ggactatcga tcccatcaaa aatccgttga
     8641 tattttttaa cctgcattcc ttcagggacg aaaataaaat ctatacgcaa tttttctgta
     8701 ttttgttccc aaccatctat tttcttttcc acagtagctg cttttccttt ttcattcgcg
     8761 acaacatacg aatcttgaag aagaaggcaa ctttctctta cagtataata accttgagag
     8821 tctactggat tattgaaatc acctaggaaa aaaagtggta cgcgagtttc caaaaagtag
     8881 ttttccaagt ttttccactc aaaataaaag ccactttttt tatctgtcca ccaagaaaag
     8941 tggcagctcg agacaaaaat gtcctgatca tctatctttg ttttcccaat caagatcttg
     9001 cgtgtctgat aatcttcttt cttttgaaat tgggagacgg taaggacctg actttccaat
     9061 gtttttttcg taagcagtgc atttccttct tcatatttat cataaccgat gtggctcatt
     9121 tcccaactcc aaaaatactc ttttccgtgt tcctttaaaa gctgaactaa acggtaggca
     9181 aaattatctt tacggatagg tacttgattc tttaccggac agaacttcat tagcttcgca
     9241 ggttctactt cttcagattc cagtaattgg ttgatttctt gtaaagcaat cacttcataa
     9301 tcttctaaca ggatcttatc tgctaattct tgcaattttt tctctggttc tggctcaagc
     9361 cagctatgcg tgttgagtgt catgaatttc attggttttc tccttgtctt catttcgaaa
     9421 caaaagggtt gtgacaaaat ttttgtcaca acctctccag caaatagtgt tctcctgcta
     9481 actcctcagt aataagctca atggaccagg agctattttt tgtttggctg tttttatcac
     9541 ttggtgcagg gcagcttttt cacgacctct ccactaaaca gtattcaaac tgctgcttct
     9601 cggtaataag cccaaccaaa cgaaaaatag gaggagctat ttttgtttgg ctgttcttat
     9661 gacttgatgc aggacagctt tttcacaacc tctccttaca ttttaatgga gccgatcact
     9721 ttaccagctg tcactgtacc taattgcttg atcactactt cttctacttt atcgtttgta
     9781 aatacgatca tgatcgttgg atctttttct tcgttttgga tcacatcaag atccatctca
     9841 gctaatttcg ttcctgttcc tacttcttgg ccttctttga cagcaatctc aaaagctggc
     9901 tgattcattt ggactgtatc gattcccata tggatcaaaa cttcgatacc atctttcgtt
     9961 tccagcccaa ttgcatgttt gctagggaaa atacttgtca cttttccagc gatcggcgca
    10021 acaacttctc ttgatgttgg aataacagca aagccgtcac ccatcatttt ttgtgagaat
    10081 accggatctt ttacttcaga taaaggaatc acttctccag aagctgcgga aactagttct
    10141 tgttctatac ctaagtattg cttactacct gcttgtccat ttcctgtaga aaccatttct
    10201 ggctctggaa tctctgctcc tgattgcagc agatcttcga tatctgattt caagacatct
    10261 gcttttggtc cataaactgc ttggactcca ttgtctttga cgatcaatcc catcgcccct
    10321 gctcgtttcc aggcttcttc agtccctact ttttctcgat catttacact tacgcgtaac
    10381 cgagtcatac aagcatcgac atctttgata ttttcttttc ctcccagcaa atgaatgatc
    10441 tggaccactt gctgatctgc tgttcctgtt ccgctgttag atacaacggt ttcttcactg
    10501 tcattgtcgt aatttccgtt acgtcccggt gtagcaaagt tgaatttttt gatcaagaaa
    10561 tttgctaaga aatacgtaac aacaccaaaa gcgatcacac acaacacgaa gttgataaga
    10621 tctccaccta gacccgcttt gatagctaac ggtgtccgag tcagcaattc gatattccca
    10681 aatgaatgga cccgcaatgg caagatatct gccatcgcaa atgcagcccc ttggatcact
    10741 gaataaatga catataatgg cacagcagca aacataaaca tgaactccaa tggttctgtt
    10801 acacctgtta gaaagacagc caacgctgct gaaaaataca ttgatttata tcttgctttc
    10861 ttatcaggat cgacattacg atacatcgca aaagccattc ccatcaagat acctgaagaa
    10921 ccaatcattt gtcctacttt gaaacgagcc ggcgtccagt tatgcaacac aaactcatat
    10981 tgcgacatat cccctgcacc tttaagattc actaaatcag tggcccaagc caaccataac
    11041 ggatcttgcc caaatacttg tgtccccgcc tgtgcgcctg ataaaatctc atacgatccg
    11101 ccaagctgtg tatagttgat aggaatcgtc agcatatgat gcaatccaaa tggcaatagt
    11161 aaacgttcta atgttccaaa taaaaacggt gctagaatcg gtgcgctttc ttgtgattct
    11221 gcaatccata aaccaaaatt attgattcca gcctgtacgt ttggccacac cacagcaaga
    11281 atcaatgcca cgattgttga ccataagata acaacaaacg gaacaaagcg ctttccattg
    11341 aaaaatgaca aagcatccgg caattttcta taattatagt acttattata agccattgcc
    11401 ccgacaaatc cagcgatgat ccctacgaat actcccatat tcaaagctgg tgcttccaat
    11461 acacttgtaa aaaatccttt aaccatgatc ttcgtaccga acagagtgtg ggtaaatgca
    11521 tcttcattcg caagcatgtc acttgtgaca ccaaagatag ccccagtgat ccgattgatt
    11581 aaaataaatg agatcccagc tgcaaatgct ccgcctgctc tttcttttgc ccaacttcct
    11641 cctattgcta aagcaaataa aatatgaaga ttaccaataa ttgcccaacc aatattttca
    11701 attactccgc ccgttgtgac aagcggcgtc caatcaggat tgatcaaggg aattgatttc
    11761 cctatactga tcatcaaccc agctgccggc ataactgcta ccacaaccat taaagccttt
    11821 ccaaattttt gccaaaactc aaaactaaat atctttttca ttccgtttag ctcctttacg
    11881 caatcggttt cgttaaacgt attatagcgt gcgttttctt ttttgtaaat gctttatttt
    11941 ttatttttct gtctatacca ttctataaaa cctataatac gtgtgttccc ttctataatt
    12001 ttctcctaaa aatttatttt tttaagcttt tttctatgaa atattttctt ttgtctattg
    12061 acttgtgcaa tcggttgcgt ttattatgaa cgtataaaac agctcattag gaggagaaaa
    12121 atgaaacaga tcaaacgact atttgctatt gatccatgga aaatcagcac gacacaatta
    12181 gataaaaaaa atcttcgact gcaagaatct ttaacaagta ttggaaacgg ttatatgggg
    12241 atgcgcggga actttgaaga aacttactca ggggaccacc atcaaggtac ttatttggcg
    12301 ggtgtctggt atcccgataa gacacgtgtc ggctggtgga aaaatggtta tccagaatac
    12361 tttggaaaag tgatcaacgc cttgaacttt atcgcgatag atatttcgat caacgatcag
    12421 ctgatcgact tggcttcttt ggaaccagaa gacttttact gggaattaga tatgaaaaat
    12481 ggtgtattat ctcgtacatt cacaatcact acttctacta ataaagtacg catttccttc
    12541 gaacgttttc tgagcattgt caaaaaagaa tcagcttata tctatttaac tatagaaatg
    12601 cttgaaggta gcggaacagt caaactcgta tcaaaattgg atggaaatgt tcaaaacgaa
    12661 gacagcaact atgatgaaca tttttggaat gagattgatc gtggaactat agaagaaatc
    12721 ggctatttga cgactaaaac tattccaaac aactttggga ttgaacaatt tactgttact
    12781 gcagcaatgc gccatttcgt taatggaaaa tcagtcatcc ctgactatca cgaagatact
    12841 ttagcattac atggttgctt gtctgcagaa ttatctcaag gtgagacatg gtcagtggaa
    12901 aaagaaatta tcgttcttac tagtagagat attccagaag cgcagcaagc agaaaaagct
    12961 gtctcagaat taacgaacca gacaaacaat tatatccaag caaaagaaga gcaaacagcg
    13021 gcttgggcag aacgatggaa gcttgccgat gtggtcatcg aaggagacga agaagcacaa
    13081 caaggtattc gattcaattt gttccaacta ttctctactt actacggcga agatgaacga
    13141 ttgaatatcg gccccaaagg attcacagga gaaaaatacg gcggagccac ttactgggat
    13201 acagaagcgt atgctgtccc tctttatctt gctttagcca aacctgaagt aactaagaat
    13261 ctgctgaaat atcggcataa tcaactgccg caagcaatcc acaacgctca gcaacaagga
    13321 ttaaaaggcg cgctttatcc tatggtcacc ttcactggtg tcgaatgcca taacgagtgg
    13381 gaaatcacat tcgaggagat tcaccgaaat ggcgcgattg cctatgcaat ctacaattat
    13441 acgaattaca ccggcgatac ggaatatttg aaaaatgaag gactggaagt tttagcagaa
    13501 atcgcacgct tctgggcaga ccgtgtccat ttttcaaaac gagcaaataa atacatgatc
    13561 cacggtgtga ccggcccaaa cgaatatgag aacaatatca ataacaattg gtatacaaat
    13621 acgatcgcag cttgggtctt gcgttacacg cgagaaaatt atttaactta tcaaaaagaa
    13681 gctgctatca ctatcagcga agaagaacta gccaaatgga aagatatcgc agagaacatg
    13741 tacttcccag ttgacgaaga attaggaatc tttgtccagc atgacacatt cttagataaa
    13801 gatctcatgc ctgtatccga tattcccaca agcgatcttc ctctgaatca gcattggtct
    13861 tgggacaaaa tccttagatc ttgcttcatc aagcaggcag atgtactaca ggggatttat
    13921 ttcttcaatg atacttttac aaaagaagaa aagcaaaaaa actttgattt ctatgaacca
    13981 atgactgtcc atgagtcttc cctttcgcca agtatccatt cgattttagc agcagaatta
    14041 ggaatggaaa gcaaagctgt agaaatgtac cagcgaacag ctcgcttgga tttggataat
    14101 tacaataacg acacagaaga cggcttgcac atcacttcca tgactggtag ctggttgtcg
    14161 attgtccaag gatttgctca aatgaaaact gcccatgatc agttgagatt cgcaccattt
    14221 ctgccaaaaa cttggaaagc ttattctttc catatcaatt atcgcggacg tttattgttc
    14281 atctcagtca cagatgaaca agttactttc gagttattag aaggttctcc tctagaaatt
    14341 tacgtctatg aacagtctta ttctttagcg gatcgtctag aaataacgct aagaaaggag
    14401 gaagcaaatg tttaaagggg tattgtttga tttagacggt gtcattaccg atactgctga
    14461 attccattat cgtgcttggc gaaagcttgg tcaggaaatc ggcattacga tcgaccacac
    14521 attcaacgaa cagctaaaag gcgtcagccg agaagactca ctctctttgc ttttagctta
    14581 tggcggaaaa gaacactctt tttcaaagga agaatttgca gaattagcga aaagaaaaaa
    14641 cgattattat ttagagatga tccagacgat cgaaccaaaa gatgtcttcc ccggggtcgt
    14701 gcctttactg gattctttaa aagaagaaaa aatcaagatt gctcttgctt ctgcaagtaa
    14761 aaacgggcca ttcctacttg aaaaaatggg tcttacacct tactttgatg ctattgccga
    14821 cccggcagaa gcaacaaacg gaaaacctgc gccagatatc ttcctccttg cagcaaaagc
    14881 tgtcggacta aaagcagaag aatgtatcgg gatcgaagac gcgcaagccg gtatccaagc
    14941 catcctctca agtggcgctc aaccaattgg cgtgggacgt gctgaagacc ttggagagga
    15001 gatcccgctt gtccctgata cgacttttct tacgatcgat tacttgaaag aaaagtggca
    15061 tgaccatgga taatccagag atctccgtcg taccagttcc agagactgaa ataaagaaaa
    15121 tcacattgaa gaacgactac ttagaagtcg ttcttctcaa ttacggtgcc cgcctgcacc
    15181 aaatcttcgc accagacaaa gaaggaaaat cagaaaatgt tttattgtct tacgatcgtt
    15241 tcgaagatgt tctaacagac aaaagctttt ttggagcaac tgtcggacct gtagccggaa
    15301 ggatccggga tgcttcttgg ggtgagcata gattggagaa aaactgcgga tcacaccata
    15361 tccacggtgg cacgaaaggc tggtctttcc aattttggga agtagaagtt ttcaaaactc
    15421 catcatcaat aggtgtggtc ttttacttaa aagatgaatt ttctacctat ccaggaccaa
    15481 tcactgctac gatcacttac cgtttaacaa aaaacgaatt agagatgaca acctctgcta
    15541 gtagccttgt tgaaaccatc tgcaatccga ccaaccatgc ctacttcaac ctttcaggaa
    15601 atggaacaag agacatctat gaacatcagt taacggtctt tttagatggt gtgcttgaat
    15661 tagatcaaga aaaactgcct actggaaatt ggacaaaaaa agaaaaactg ccaatcgact
    15721 ttagaaaatc cccaacactg caagagatcc ttgcatgtta tccagatgga ctagatgatg
    15781 tatttctttt gcatcatcct aggctttcca aaacttcctt acaattattc gagaagcatt
    15841 ctggtcgcca aatgactata gcaacttcta ataaaagtat ggtattattt tctacgactg
    15901 gattcgaagc tgatttctct gtcaatggaa aacagatgca ttcaaactac ggattagcaa
    15961 tcgaaccgca agaaatccct gatatcgttc atttccccaa atttggatcg atcaacctcc
    16021 atcctggtca agaacggatc agtcagacga tttatcggtt ctctgcacaa taacaagaga
    16081 aaggtgtttt tatcatggct attaccgtaa aagatgtggc gaaaaaagcc ggtgttgcca
    16141 cctcgaccgt ctctcgtgtc atcaacgatc atcctagtat ttccgagaca acaaagaaga
    16201 aagtccgaaa aattatggat gaactaggtt atgttcctaa catgactgcc cgcaatctag
    16261 gaaaacgcat ttccagtgcc atcggcgtaa tcttaccccc tcttgattca aaagagcgga
    16321 tcggtaaccc attttatctt gagatcatgg aagcaatcaa cgaagaagcg cggaattatg
    16381 atatgactac agcaatcgct actgctaaaa attttgaatt gctgttagag aacgtccagc
    16441 gtatgcattt acaaaaacaa gtagatggtt ttattctagt gtattcagat agtcaagata
    16501 ctgtcattga ttatttatac caaaaccaca tccctttcac tttgatcgga cagccttatc
    16561 atcatgagaa cgaaatcgtt tatgtagata atgataacca gcttctaggt aaacaagcaa
    16621 cagaattttt gatccagaat ggacataaga atatcctgtt tgtgacaaac acgactcatg
    16681 aaagtttgta tttcgaacga tatttcggtt atcaaaaagc aatgatgatg gctggtcttc
    16741 ctgcgttatc cagtgtagta ctagagcatc cggaggatta tctgtccttt gagaaaacgt
    16801 tgaaagaaaa cgaagccaca gcattggtgg tcattgacga tatcttcgcc cttcgcacga
    16861 tgcagttggc gcaaatgtat ggctatgtca ttccagagac actctccgtc attagcttca
    16921 acaactcgat cttctcaaca ttaacacacc cttaccttac aagtatcgat atcgatatct
    16981 ccgaattagg acgtgtcgct atgcaaaagc tgcgcgaatt gatccatgaa gaattgagca
    17041 gtggcgtacg tttggtcatc cctcatcgct tgatcaaaag agagacagtc gttcctttag
    17101 aaaacccata ctaaatagct gactcactaa atacgctgac catttatcct atggtcagct
    17161 ttttatgtaa aagaagatac tacttctaac acattgttta tctgtaaaaa agagcgtggg
    17221 acaaaagtaa agaacacttt tgtctcacgc tctaaacacg tataaacggc ggaagcagaa
    17281 gcaactcctt cggaaataag ctgaaattca caaaaattta aaaagcaatt ttcttgagtt
    17341 tcttcttatt tcttggagtt aaacgcttct gtcccagcct cttttttcct tccttcaatt
    17401 ttttacacgt cgacgattca ataaaaaacc gcctaatatt gtaaagacag gtgcaagaat
    17461 cgagaaaaag gcgaatggca agtaatgcaa cgcgctaact ttcatcgttc ccataatgaa
    17521 tgtgccgctg actccccacg gtacgagaga attgacggtc gcacctgaat ccgctagtgt
    17581 tcgtgtcaaa taggtttttg ataagccaac gtcatcaaaa gaacgcttga atgtttctcc
    17641 tggaagaatg atggacagat actgttctcc aacgatcaga ttgattccga tacagctcaa
    17701 ggcagtaaac ccgactaaac gagaaggtcg gtccatcttt tcttttaatt cttgaacgat
    17761 ggtatcaacg atcagatatt tgatcagtaa cccacctaat gccaatgcaa gtaaaatcaa
    17821 actagcagaa cctaacatac tcatgatccc gcctcgagaa agcagttcat ctagctttgg
    17881 atcaggtgta tttgcgacat aaccattcat caagatgtct gctgcttttc cgaccgtcaa
    17941 acttttctca tggataaaag aaagaacgag agcagctgtc gatcccgcta gtaatgtagg
    18001 gatagccgga atttttttga ttgccccccc aaataaaata actagtggaa tcaatgtcca
    18061 aggagagatc caaaagctcg ctttcatgtt ttggatcatt tcattgatac tagctagatc
    18121 ggctgaatga ctgttatgtc ctaatatcgt ataaaaaatc aacgaaagga cgaatgctgg
    18181 gaaatccgta taacgcatac cagcgatatg ttcgaataga ttaactcctc caatacccgc
    18241 tgccaagttg gtcgtatctg ataaaggcga gcagttattc cctaaaaagg caccggaaac
    18301 gactgcgcct gtagtcattg catcattgaa tccaagtaaa tgaccgatcc ccaaaaaagc
    18361 aattccaatc gtcgaaatcg ttgtaaatga actgccgaca gtcactccga caattgcaca
    18421 aacaagaaat gctgtcggta aaaagaaacg cacagaaaca agtttaaatc cataaaccat
    18481 aatcgtaggg atggttccag ataaaatcca tgaagcaact aagacaccaa tcaaaagaaa
    18541 aatcaggatt ggaatgatcc ctggagtgat tcctttgatg atcccgtcat ggatctcatc
    18601 ccatgtgaac tttttaaatc tgccataaaa caacaaacac atgaaagctg caaggatcgg
    18661 gatatgtggc gagagctgga atccgatgat cagtactcct aggagtgcca acaacacgat
    18721 caatacagcg atactttctt ttaaagaaat atttttattc atcgtcgatc tcctatttca
    18781 aatgaaaagg ccctgccatg aaaaaactat tcacttggca ggacctccta ctaaatcagc
    18841 gacaaaacgt cggctgaaag acttattttt ttatttttct tctaaacttt tttctacctc
    18901 aatcccccaa gaatctttct tgaagtgatt gatgatcaat ggaataacga acatgaccac
    18961 tgtgatagct gctactaata atgcatcact caatgtcgct gtacctaatg tagcaaatac
    19021 gacaacgatg atagagaaga tcagcatgca gctgactacc caagctaatc cgtttccgtt
    19081 tttaccaatt cggtaaggac gttcgaattc tggatgtttc ttacgcattt taagcaatga
    19141 aacagcaatc aaaatgtaca cgatacaata aataacagtt gtagcatttg taagtgtcaa
    19201 gaatacagca cttacgtcat ccattacacc ataaagcaaa gcaaataatg aaataacgat
    19261 tgattgtgtt agaacgacat ttctagatac gccgtatttg ttttctttat ggaatccgaa
    19321 ttttggtgga aggaatcctt cacgagctac ttggatgatt gtttttgatg gtccagtaac
    19381 ccaagctgat aattgtaaaa gcacaccaat aaataccatg aagctgaaga tatttccgat
    19441 cacttctggt aagcctagga tttgacagtc aatcaagata ggctgcgtga tatttgctaa
    19501 ttccattttt ccatctggta cgacatttgc tacaagcatc gcattgatga cattcaataa
    19561 aactaatccg atcaaagcga taaatacgcc ttttgtataa ttttttgttg cgtctttcaa
    19621 acgaggcatg taaacagaac tcatttcgat cccaacaaaa ataaatgtga taccagctaa
    19681 gtatttcaat gaatcaatat gttccaagtc agggaaagct tttgaccatg aaaagtcacc
    19741 tagatagccg tttgagacta gtccgacttt gaaggctgcc aaaacaccta aaacgaacat
    19801 gatgactact gggatatata cccctagcca tacaccgatg ttcccgccga cttttgccat
    19861 atcgaatttc aagttcaaga tcgtgatgat ccagtagata actaaaatac aacctaaaac
    19921 gaaccagtga ttattgccaa gttctacatt accaaatgtg tttcctaaca atggtcctaa
    19981 agtagaagcg accattacca tacctgggaa catttgcacc cataatagcc atgcaacgac
    20041 aaatccccat ttgctgccca gtgctgtttt tacccataac tgaggaccgc cttcttgagg
    20101 aagcatcgtg gaaagctcac cggaaaccat tgatatcgga ccggcaaaaa acaaaactgc
    20161 aaaaattgaa taaaaaatca atgtccaacc gactgcggct aacgtaggaa tactccgaac
    20221 cgttgcacac agtgccattg tcagaccgat aaaggtccca agacccattt tacttacttc
    20281 agatttactc accgttgaca ctctccttga ttttttggat aaaataacgg tgtctttgaa
    20341 gtagtttcag caataaacga aacaattcag cgaatatttg aggggattct ttgagtttat
    20401 tgcttgaagg gtttcttcaa agacaacctc aaaacaaagg gatcgaagag cttaggtctc
    20461 cccttgctct tcagatccaa acatgcgtca gaaacagtta gtattcaagc tgttttctaa
    20521 catgattacc ttgcttattt tgcttcgctt gccaataatt ggtctgcgta gattttttcc
    20581 aatttttctt gaagagctgc tttgattttt tcagcatatt cttcaaagtg ttcttctttg
    20641 ttcatgtatg gtgtcattac gctggcacgc aatacagtta cttttccggc acggttccat
    20701 tcttcatctg agaatcctag ttggttcaca aattgtaatg ggctgttgcc atagtctggg
    20761 atagcaaagt cagtatgtga agttaagaac tcattaccgt aaatgcttcc tttcacgtat
    20821 gaagaataat catatacgtc atggttcaat ttattcattg caactaagtc atcgttgcct
    20881 ttttctttga atacatagtc taccatattg aaatctggat aagttaatgg atgaacttcg
    20941 atttctttgt ctcctacttt aaatgacaag tcattcaaga agttgtagaa acgatgtgca
    21001 ccttcgattg atgcacccat taatttacca tatcctgtga cattcaaagg taagacatgg
    21061 tgtgctgccc aaacgctggc tgctgttgcc cctgcttttg aaccttccaa gatataagcc
    21121 ccaagtaatg ctgggatatc tgcgcctttt tcaaatacat atgtcgcaaa gtaagagata
    21181 acgtctctca tgcggatatc tttgatgacg ataccaccgg cagaatatgg aacatagccc
    21241 attttatgtg ggtcaatcgt tacagattct gcttcttcga ttgctttata tgcactgtgt
    21301 acttcttcta agatataatc tttgttttct gtaaagacat tgtatttgta atgaacatct
    21361 tttaaatctt caaatggaat gaagttgttg tcttcgtcta agaaaatcgc acggccgtat
    21421 ccgccataag cagcatctac gtgtaagtag aagtaaatac cgtctttttc taatacgcga
    21481 cgcaatgcaa cgattttatc gataccgtcg attgctcctt cttctgttga tcctacaaca
    21541 ccgactacac ctaggattgg tgttttttca gctgctagac cacgaacgat tttttcaagt
    21601 tcgttgatgt ccatgcgata gttgtggtca actggcactg gaatcacttg gtctaaaccg
    21661 ataccgatga tgtcggcagc ttttagccat gaatagtgtt tagtttgtgg aaccaaccat
    21721 tttcctaatt tttctaaatg ttttccgcta cgtgctgagt gagctttgat ttcgtcgatt
    21781 ttttctggga cgctgtctaa cagattcatg atttcttctg ttgacaagtt cattaattcc
    21841 caatcgcttt ttccagcaac tagttcaggt gtcacttctt tcattgcaag cggcaatgat
    21901 ttgatgttgc gggcatacca aagtccttct aagtttgcta aagaaccatc agcaacgatg
    21961 tggccccagc catctttata gctcattaat tttgcaaatt ccattcctac ttcttcttcc
    22021 atttggcttg ttgcaggaga agattcatag gcaacgttat taccattcca tagcatagca
    22081 aaattgtacg ctagtaaaga tggcatcaat gtttcagagt tcatatgacc ccagtaacgt
    22141 ccggcattat gccaaggaac ggaatgggta cgcatccgcg ctgaaatttc tgatagaaca
    22201 tcctttgttt tattcacagt gtgttcaaaa ctagcactgc ttttttcttc tggcgtaata
    22261 ataggcatgt cttgaggcat gtagttttga cgccagccta aatgttcatc caccaattca
    22321 ttcaacaaag ctttatagat ttggccgttc tcagctttgt ccccaatgaa tagggcattt
    22381 aggtttagat ctttcgacaa tgattcactc atagtgaaac cctcctaaaa aaatatttaa
    22441 attttgtcga gccctacatt acacctgttc ttctattctg taaactaagt tttggcctga
    22501 tttttaatca tattttaata tgtatcaatt atccgaattg ttcacacttt tgaaataatt
    22561 aaaaaaaacg agggaattta caaatgtaaa ctccctcgca aagattgtat taacgacaac
    22621 cagcgttcta ttttaggatg ggtttcaaaa ttggaatgtt gtaatagtat ttcccttttt
    22681 caaggtaaaa acttactaaa ttctaatcaa ttactttggc caaaaagtaa tttttcttac
    22741 cttttcgaat aaccacaaac ttaccatcga attcatcgga tggatttatg acaaaattta
    22801 aatcggtaac gcggtcaccg ttaatactta tagctccgtt tgaaacatct tcacgtgctt
    22861 gacgtttaga tggttcaatt tttgtggaaa caagcagttc cacgatattt tcaggtgtgc
    22921 ttgaaatttc aacgttcggc attttaccga atccttgcgc gatttcttcc gcatttagtt
    22981 ctttaatatt tccagagaac aatgcttgcg ttattttttg cgcttctttc aagtcttctt
    23041 cactatgcac aaatcttgta acttcttctg ccaaacggcg ttgtgcttca cgtttctcag
    23101 gttctgtctc tacttttttc gctaagtctt cgatttcttc ttgagacaag aaagtaaaga
    23161 atttcaagta tttcactaca tcgcgatcat cttgattcaa ccagaattgg tagaactcaa
    23221 atggtgaagt tttcttcgga tcaagccaaa cggcaccgcc tgcagttttt ccgaatttcg
    23281 ttccgtcagc cttcagcatc aatgggatcg ttaaaccgaa cgcttctgct tctggtcctt
    23341 ctaattttcg gattaggtct aatccagctg tgatatttcc ccattggtct gctccaccga
    23401 tttgcaattg cacatcgtat gttttatgca gatgcaaaaa gtcgatagat tgcaggattt
    23461 ggtaagtaaa ctctgtaaat gagattccta cttccaaacg gctggcaacg atatcttttg
    23521 ctaacatcgt gttgatgttg aagtttttac cgtagtctct caaaaattca agtaaagaga
    23581 tttttgacaa ccagtcgtag ttgttcacaa atgtgatatt tgcatcttta ccaaacaatt
    23641 ttctcatttg attggaaagt gcgtccacgt tatgttgcac ttgttccatc gtttgcaaca
    23701 cacgttctgt tttgcggccg cttgggtcgc cgatcgttcc tgttccgccg ccaatcagaa
    23761 tatatggatg atggcctgcc aattggaatc ttttcatcat cataaatgga atcaaatgtc
    23821 cgatatgcat cgaatcacca gttggatcaa cgccgcaata aagtgaaatg ctcttttctt
    23881 caacgagctc gcgaagtctt tcttcgtttg tttgctgatt aatggcgtcg cgccaagtta
    23941 attcatcaat aatattcatt ttactgacct ccgtgtagtt tttttatgaa agcaggaacc
    24001 tgctagcatg tgtaattaat tatttcttca ggataggata ggaaataaaa aaagtccctt
    24061 ggcatataag ccaagggacg aaatctcgcg gtaccaccca atttgtatgt ctagatatcc
    24121 aacatacctc tcgaacattt aacgctgtcg cgtggcaatc actgccaaag actcctgaaa
    24181 tgtaattcgc tttaaagcat gtactggttt gcagcgacca ccagctttct gaaacattgg
    24241 ctttaacaac tacttcgttt cgatcttcgt cttgttatta ttaatatgtg tctatcatag
    24301 agcgcaactt tttctttgtc aacagaaaaa ggatattttt tttaaattcc tcattttttc
    24361 ttttttgaat ccgctctaat tccgaacatt ttattttcaa ttattgttaa aagaaataac
    24421 tttttctgta ttttttctgc aatttatcga gaagtcactt tactttgcct cttattcttt
    24481 cttcgttaca ataaggaatg ggacaagtaa aaaaatgaaa aagcaaggga cgaggtatat
    24541 ggaaaaaaag caaaaaatta ttttaggagc cacttctgct ttaactatcg ttattttaat
    24601 tctaagtttg ttttttgtca tccgatcatc tacaaaaaaa gaaccaatca ttcctgaaaa
    24661 aaaaataaca gaaaaagcaa aagaaccgac agatgataag caacaactag ttactcaagc
    24721 aaaaaatctg gctcaaagct acgattatga ccaagcgatt gcgttattgg aaaaagatag
    24781 taagaacgga tctactgaaa cacaacagct tcttcaaaca ctaaaaaagg agaaagaaca
    24841 actgatcgac tggtctgatc ctactcagat ctctcatgtc tttttccaca gcttgatcgt
    24901 ggacccgcaa aaagccttcc actctcaaca ggcacaagga tacaaagatt atatggtgac
    24961 tgttgaagaa ttcaaccgtt cgatcgaaca actttatcaa aatgattttg ttttagttaa
    25021 tttgaatgat ttgatccaaa aagacgaaca aggaaatctt tcctttaacg ggcttgcgct
    25081 acctgaagga aaaaaaccat tggttctttc acaagatgat gtcagctatt atgaatatat
    25141 ggataattcc ggttttccta gccgattggt cgtatcaaat aacaatgaaa tcaaaaatgt
    25201 ctataaagag aaagataaag aagaaatcgg tgactttgac atggttcctc tgatcgatgc
    25261 ctttgtcaag aaacatcctg atttttctta tcgaggcgca aaaggtacct tggccctgac
    25321 aggttacaac ggagttttag gttatcgaac atcaaaatct caatatggag acaatgaaaa
    25381 aacaaaaaaa gagattaaag aagctaaaaa agtggcggaa cagttgaaga aagacggctg
    25441 gagctttgct tcccatacat ggggtcactt gaatatgact caagcttcac tagaagatgt
    25501 aaaaaaagac aatgaattgt ggcagcaaga agttgcacct atcctaggaa aaacaaatat
    25561 tctgatttat ccatttggtg cagacatcag cgactggcag ccttattcag cagaaaatca
    25621 aaaatttgct tacttgaaga gtcaaggttt cgatattttc tgtaatgtcg atgcttctac
    25681 accagcttgg ggacaattag gtacggattt ctatcgaaat gctcggatca atatcgatgg
    25741 cattcgtttt caatctgatt tagacggaaa aaatcctatt cttgatccat tcatcaatgt
    25801 ccatgatgtc tatgacaaag aagcacgcga atctgcttga ttttacgtca tttatgaacc
    25861 aaaaaagccg aactgacatt t
//
LOCUS       DAXWOO010000023        24875 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.023, whole genome shotgun sequence.
ACCESSION   DAXWOO010000023 DAXWOO010000000
VERSION     DAXWOO010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.023"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..808
                     /locus_tag="ACGE70_000767"
     CDS             <1..808
                     /locus_tag="ACGE70_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFF8692422.1"
                     /translation="GYELDATPIEFEIERSQTAVVELTKANRLTPGGVVLTKIDDQSG
                     EVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPI
                     EFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLT
                     TGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVAD
                     SSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(1161..2009)
                     /locus_tag="ACGE70_000768"
                     /pseudo
     CDS             complement(1161..2009)
                     /locus_tag="ACGE70_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2162..3274
                     /locus_tag="ACGE70_000769"
     CDS             2162..3274
                     /locus_tag="ACGE70_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFF8692423.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3352..3645)
                     /locus_tag="ACGE70_000770"
     CDS             complement(3352..3645)
                     /locus_tag="ACGE70_000770"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFF8692424.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3813..4127
                     /locus_tag="ACGE70_000771"
     CDS             3813..4127
                     /locus_tag="ACGE70_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFF8692425.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4148..6127
                     /locus_tag="ACGE70_000772"
     CDS             4148..6127
                     /locus_tag="ACGE70_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFF8692426.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6140..6466
                     /locus_tag="ACGE70_000773"
     CDS             6140..6466
                     /locus_tag="ACGE70_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFF8692427.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6482..7000
                     /locus_tag="ACGE70_000774"
     CDS             6482..7000
                     /locus_tag="ACGE70_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692428.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            7000..8352
                     /gene="celB"
                     /locus_tag="ACGE70_000775"
     CDS             7000..8352
                     /gene="celB"
                     /locus_tag="ACGE70_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFF8692429.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8436..9941)
                     /locus_tag="ACGE70_000776"
     CDS             complement(8436..9941)
                     /locus_tag="ACGE70_000776"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFF8692430.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(10033..10623)
                     /locus_tag="ACGE70_000777"
     CDS             complement(10033..10623)
                     /locus_tag="ACGE70_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFF8692431.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10802..12448
                     /locus_tag="ACGE70_000778"
     CDS             10802..12448
                     /locus_tag="ACGE70_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFF8692432.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12497..13216
                     /locus_tag="ACGE70_000779"
     CDS             12497..13216
                     /locus_tag="ACGE70_000779"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFF8692433.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13695..14642
                     /locus_tag="ACGE70_000780"
     CDS             13695..14642
                     /locus_tag="ACGE70_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8692434.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14654..15574
                     /locus_tag="ACGE70_000781"
     CDS             14654..15574
                     /locus_tag="ACGE70_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8692435.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15615..17072
                     /locus_tag="ACGE70_000782"
     CDS             15615..17072
                     /locus_tag="ACGE70_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFF8692436.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17417..18031
                     /locus_tag="ACGE70_000783"
     CDS             17417..18031
                     /locus_tag="ACGE70_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFF8692437.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            18024..19748
                     /locus_tag="ACGE70_000784"
     CDS             18024..19748
                     /locus_tag="ACGE70_000784"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFF8692438.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19745..21196
                     /locus_tag="ACGE70_000785"
     CDS             19745..21196
                     /locus_tag="ACGE70_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFF8692439.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(21669..23822)
                     /locus_tag="ACGE70_000786"
     CDS             complement(21669..23822)
                     /locus_tag="ACGE70_000786"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFF8692440.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIRQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(23824..24870)
                     /locus_tag="ACGE70_000787"
     CDS             complement(23824..24870)
                     /locus_tag="ACGE70_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFF8692441.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
BASE COUNT         7931 a         4308 c         5012 g         7624 t
ORIGIN      
        1 ggggtatgaa ttagatgcga caccgatcga gtttgagatc gaaagaagtc agacagcggt
       61 ggtagaattg acgaaagcaa accgacttac accaggcggt gtcgtgttga caaagatcga
      121 tgaccaaagc ggggaagtcc tgcaaggagc tgtttttgag ttacaagatg ccaatggaac
      181 cgttctgcag tcaggtctaa ccacaggagc agatggaaaa ctggcgattg acggcttagc
      241 accaggagtg tatcagctgg tagaaaccca agcgccaacg gggtatgaat tggatgcgac
      301 accaatcgag tttgagatcg aaagaagtca gacagcggtg gtagaattga cgaaagaaaa
      361 tcgacttaca ccaggcggtg tcgtgttgac aaagatcgat gaccaaagcg gagaagtcct
      421 gcaaggagct gtttttgagt tacaagatgc caatggaacc gttctgcagt caggtctaac
      481 cacaggagca gatggaaaac tggcgataga tggcttagca ccaggagcat atcagctggt
      541 agaaactcaa gcgccaacgg ggtatgaatt ggatgcgaca ccagtaactt tcaagatcga
      601 aaaagaacaa gaagtggcga tctttttaac aaaagagaat agaaaagttg ctgatagctc
      661 agatataaga ggaaacaatc caacaagcag tggaaataag catcttccaa aaactggaga
      721 aaccatcatt agccagttta tattgtcaat attgggtgtg ctacttgttt tcatcagtat
      781 aaaatttaga aagaaacgaa atatatgagc taaaataaaa aattgaataa gcatagtttt
      841 taaaatcaac tagtacccat caattgaatg gaaactaccg attttagaag gggtatacaa
      901 acaaatatta gtatcggagt agagaaggtt gaacccaacc agtaaataag taaagacaat
      961 tttgtgctgc gttgagaagt gaaaaataaa cctttgtagt aatactagtt aataaaaaat
     1021 atacaaaaac actgcgaaaa tcattcttga gtttcgcagt gttttttgcc taatgactca
     1081 agtttattgg gtaatacttt gattttcttt ctacaaataa tcagcgatca gttgtaattt
     1141 tttctctagt tttaatggct ctttgtcaat aaggagaaaa acaagtcggg tcttttgtct
     1201 cggaaagctt caaccaactc gtgaataaag gttacgcctc cttgaaaggc gtggaaaaac
     1261 caagtaatcg atcaatcacc aatttcgcat ttggtagaac atgtttggtg agctgaaagt
     1321 aggtggcgtt catgtctgta actaagaatt ctatttcttt aggattagtg cagctaagga
     1381 aatagtttgt taatcgagga attttttgtg taggtaaatt ttctcgatta atttttctgt
     1441 ctcgccatcc gcggaaataa aactcagttt atcctcaacg gaaacgtgag aacgaaattc
     1501 acaaccctca acactcaaga cagaattctt ttggaagact ttggtaaata gcttctcaac
     1561 tctttcaatg tgcgaataac ggttgtcaaa gatacctaac aacttttcga aataaaaaat
     1621 aacgacactt tttcagtcag taaagaagtg attttatgtc tgacatgatt tgaaatcgag
     1681 tgccttggac ggataaaata actttgagcg ttccaatggc ttcgacagtt tttacaggta
     1741 tagcgctgct tttttaggcg tataaccatt ggcatatgat tatattattc aaagtggaca
     1801 atggtttcct tcttcttgtt tttcaccacc attaatttcc tatttccatc taccacagca
     1861 gaaccacaat ttctacagac acatggagca gggaagagaa ctgcagcaac gatcaatgtt
     1921 tttttcttct gtaaggtttc gtaagagacc tctgtaatca tcaaattttt gtctattgat
     1981 ctcagaattt ttttgataga accattcata aagcatatcg ttctctcagt tgtttatttt
     2041 gtggtgattt aatcatacaa gaaaacgata tgttttttta taaaaacatg aataggaaaa
     2101 atagttgaca agaaaatgac aacgccatat gatgttccat agtatttaca acctgtcata
     2161 gatgactgta aaaaataaaa gtaaaaagaa aggacgacaa caggtggatt tttatcagtc
     2221 tctacaacta gatccgttta ttttaaaaca aaaaatacga gaagcagctt ctaaaaaaga
     2281 aaagtgcatg tatatttgtt cattgttttt acgatcatta ttcatcgtat tgtttgccat
     2341 ttgttttatc attataatta caacattatt tgaatcgaag cataaaccat atgctgtggt
     2401 tttattctgt atgttgatga gtattcgttt tgttgacttt ggttataaga tttcccattc
     2461 gatcataggt ttagcaatcg tcatgttctc actattagta gcaccgtatg tccagctgat
     2521 caaatggtca gttatgggca tgttgattca ttttatttta ctaagcagca tattaatggc
     2581 aaccgctagt gatcccaaaa tggggaatgc ttctttgtac ggcttttcat atctctttat
     2641 tgtttattcg ttacctaaag attcgttgaa caagaatttc tttacacaaa caagtagctt
     2701 attatttttg tttttttgtt ggttttcagt tctcctttac agaaaacatc gagaaaagaa
     2761 caaagacaaa agcttgttta aagagatttt tttgaaaggc atgtattcgc aggaaaaaat
     2821 ttggatgctc atctatgcat ttggcattag tcttttgatc gttgcaggag agtatgtccc
     2881 ttttcagcga ttgatgtggg cagggtttgc attttcttct attgtatcta gctatggatt
     2941 gatgtcgatt ggtttcaaag aacgagcagt agaccgaatc attggctcat tgattggatg
     3001 tgttttattt attggtatca gccaatttat tccttttaac tgggtcggta tattaggtgg
     3061 tctagcttta ggtgtttgtt ctacctatag atataaaaca gtttttaatt cttttggtgc
     3121 tttggcaatc actgccagtt tattcggggt tccaggagca gtaacgatcc gcatttttga
     3181 gaatatcctt ggggtatgtt taggaatcat gtatattggg gtgactgaaa tacttatccg
     3241 aaaaataaga gaaaaacacg gtttgaatca ttagatgaga tcgaataaaa aataaggaaa
     3301 aagtgaagat acacatggat gataaattgc tatcattcca tgtgtatctt tttacggtaa
     3361 atcagtgaaa cgttcgactt tcatttttaa tttatacttt ttacggagtt cgtttatttg
     3421 agccatcatt tcggatttgt gatgcttttc gattgcttct tcatctttcc aacggtcgat
     3481 cagtaaaaga gattctggat cttctagtgg aaagtaatag tcatatttct cgtttccttc
     3541 ttctgcacga atcgctttga caatgtttct ttcgaccatt tcttctgcaa attttctggc
     3601 ttgtccattt gtacttgtat acaaaatatt cactgtgata ctcactaaat cccctcctga
     3661 aaaaattcta tcactagtat atcacggtgc cagaaattat tattcaagat atgatacaca
     3721 ttttttccgc aatgctacgg aaatatatat tttgaataga aaacgcttac aagatatgct
     3781 tggtttataa aaataaaagg agtgattgat ttatgaaaaa agcattgatt atttgtgcgg
     3841 caggaatgtc atcctcgatg atggcctcaa agacaacgga tttctttaag caaaaaagag
     3901 aagaggtact cgtagatgca gtttcagcta cagaaggcga taatatgatt aagaatagtg
     3961 attttgattt atttctgatc agcccgcaaa cgacgatgtt tttggataaa ttcaaaaaat
     4021 taggagaatc tgttggaaag cctgttgtta gtatcccttt ccaagcgtat gtcccaatcc
     4081 ctacaggcat ccagaagtta gcagaggtga tcgaagagaa catatgatgg aataggagtg
     4141 gatgataatg aaacagaagg aaaaattgct gttgagatat ttgattgatc acaagaagga
     4201 gtatgtcacc agtcaacgtt tagcttctga attgttatta tctgatcgga caatccgtaa
     4261 ttacttgcag cggatcaaag agttagtaga aaaaaacgga ggtaagatca ttgctaaacc
     4321 aggttatggc taccaactgc atattttaca aagactgaca tttgacttgt ttttgagtcg
     4381 gcaagaaata gtacctggat atgctagaga accgcaagga ttttatgagt ccgaagatcg
     4441 acaaaagtat atattgaacc aattgctttt ggaagatgcc attcttttta tggatgattt
     4501 ggcagaagag ctttatatca gtcgttccag tttgacaaag gacatgcaag aaatcaagga
     4561 gcgattgatt ccatattcac taaaaatcgt ttctaagcat ggtcaaggga catggatcga
     4621 aggggaagaa agaaatcgac ggcattttat catggatact ttttttggaa accattatgg
     4681 aaattcattg aaagaatatt tagggcaaag ccgttttttc ccagacatca gttttgaaga
     4741 gctagtcatt atcattctcg atgaaacaag agaagctaag ttaaaagttt cggattttat
     4801 catacagaat ctgactttac acttggcttt ggggatcaaa cggctccgag aagggtttga
     4861 aatcaaggaa ttaggcatcg aacaagaagt ttctaagcga gtagagtatc aagtggcgca
     4921 gcggatcgtt caacgtattg aggtaatcgc aggtgtatcg tttcccgaag aagaaattgc
     4981 ttatttgaca ttacatctca tggctaagtc gaatcacaaa gaaaatcaaa atgatcagga
     5041 actatcaaag gaattagcga aagttctgaa aagattgtct caggaatttc atttttcatt
     5101 agtagaggat ctccagcttt tcaatggact actcgatcat ttgtcaccga tgatgattcg
     5161 attgagccga ggtattcgca ttgagaaccc attgactgag gagataaaga aagaaaatcc
     5221 caaagtcttt gacgcagtaa aaaggcattt cagcaacatg ccggctttga aaaactatac
     5281 catcaatgaa gatgaatggg cctatttggc tttgcatctc atggcggcat tggaaaaaga
     5341 aagagctgcg cataaactac atgcattgat tatctgtgca acgggttacg gcagtgccca
     5401 actattaaag aatcgcgtag tcagtgaatt cggtaaaaat attacagtgg taagtgtaaa
     5461 aggctattat gagatcaatg aaacaacttt aaaaggaatc gacctgatta tttcttctat
     5521 tgatctttcg acaatgtttt tcaaaattcc agttttacat gtcagtattt ttttgaatga
     5581 agatgatgtt cgtaaaatac gaaaggtagt tggagaatct ataccctatc attcctcaga
     5641 tagaccgata ccagcatttt ctctacagca aaaaaagcag atttacaccg aacagttgtc
     5701 tgaaaaattt ttcaagactt atacgtacga accgaaaaag gaagaaattc ttcatgattt
     5761 gctgcaaaag ctgagcataa atgaagaaga aggatatata agagaaatgc gaaagcagat
     5821 ccagcaacgt gaaaatatgg gacaaatcat attcagtgat acagtggcag tcccacatcc
     5881 cgctgttcca gttggaatcg aaacaaaggt agccgttgca ttgattcctt ctgggatgca
     5941 gtgggatcaa tatcaagaaa ttcattttgt ttttttagtt tcgccttctt atgtcgaaaa
     6001 tgaaggaatt acggtcgtga ctaaagcaat cgttaggtta gttgaccagc taaaagtaca
     6061 gcaggaaatc ttagctgaac ctatttttga gaatttcagc agtaaattta tcaaactgat
     6121 cgaataagga ggaaaacaga tggctgaaaa aataaacagt gaaacactac aagtgacttc
     6181 ttttgatatc attttccata gcggaaatgc acggacactg atacatgaag catttcgctc
     6241 gatgcgtaaa gagatgtttg aagaagtaga agagaagttg cgacaagcaa atgaagagat
     6301 tcttcatgcg catcagtcac aaactcaact tctgaaagaa tatgccagcg ggacaaaaat
     6361 agaaatggaa attatcatgg tccatgcaca agatcatctg atgacgacga tgacattatt
     6421 ggaagttgca caggaaatga attatttgta tcaaaaaaat aaatagaaag gaaggatcat
     6481 tgtgcaggat acagtaaaaa ggaaaatcga attagaaaaa aagcaactta gtttgaaaaa
     6541 catgtatttc aatcgttatc taatcgtacg ttacctgact gcctttttct ttttcatgaa
     6601 catgcaatgg atgattctgc ttctatcagc taaaagttta ggaagtagtt taccgatgat
     6661 tctgatttta gctattttgc ctgcagtagg agaacaagta aagctttatc gaaaacacca
     6721 aacgaacgtt ccatggacaa agtggtattt tttgttccag agtgtcttta atttattagt
     6781 gattccagtg ttgtttacct caggatttac attcttgtat ccttttatgg caaataacaa
     6841 tggaggacag ttatttgttt tcattcttgt tgtaagcggt atttttgcca gtgcatttat
     6901 acagtatcgt ttaaagaaaa tttcactcaa ccaagaccaa cagtatatcc gtattaaaca
     6961 atatgaaaaa gcgttatatt taggaaagga gaataactaa tgtttgattt tttgcagaaa
     7021 tacctgatgg gacctatggg gaaaattgcg caacttaaaa tcgttcgggc tgtcatggca
     7081 gcggggatgg cttctattcc tttcacgatt gtaggctcga tgtttttggt tttaaatatt
     7141 ttgccattac cgttcccatt tttagaagga ttttttaatg caactttttt caaagttagt
     7201 gacttgtaca tgatcgtcaa tacaatgacg atggggattt tatctattta ctttgccatt
     7261 gtgtttgctt atgaattaac gtcaattgaa cgagatgaac aaaaattaaa tgttaatccg
     7321 ctcacggggg cattactatc tgtttttgca ttctttatgt gtatccctga attgatcatg
     7381 actgatggta aaatcagctt gattgcttct atcaatgaga cagaaactgt agtaaatggt
     7441 attaggatgg gctcttttgt cgaacgttta ggaacttcag gaatctttac agctattatt
     7501 atgtcgatta ttgcggtcga attgtattgt atgtgtgtca agagaaattg ggtcataaaa
     7561 atgccagatg ttgttccgcc aggagtttct cgttctttca ctgctctgat cccaacattt
     7621 gtcattgcgt ttgtggtgat gatcatcaat ggattattag ttgcactagg aacggatatc
     7681 ttcaaaatga ttgctatccc atttgggttt gttacgcagt tgacgaatac atggcttggt
     7741 gtcatggtga tttatttctt gatccatgca ttgtggatcg tagggatcca tggagcgaat
     7801 atcatcactt ctttcttgac cccgatcgta ttagctaata tggcagctaa cgcacaaggc
     7861 gcaaattatc cgttggcagg agaattcaac aattcttatg taaccgttgg tggatcaggt
     7921 gcgactttag gattgatcat ctttattgca ttcctcgcga aatctgatca gctgaaagtg
     7981 ttaggaaaag cctcacttgt accaggatta ttcaatatca atgaaccaat cattttcggg
     8041 atgccaatcg tctacaatcc ctatttagcg gttccgtttt tcttagctcc gatggcttct
     8101 gctacactcg cttattttgc cattcggctg gaaatcgtca aaccgatgct tgcacagatg
     8161 ccgtggcctt caccagtcgg gatcggagca tttgttggta gtggtggtga ttggaaagct
     8221 gctgtgttag cagtcatttg tgcaatcatc gcattcatta tctggttccc atttatcaaa
     8281 ttctatgata ataaattatt aaatgaagaa agagaaaagg cagcagaatt agctagtgaa
     8341 aagttaaaat gatttctaag aaaaaaggaa ctgtgacaaa aaatcacagt cccttttttt
     8401 cttagaaatg ggaaaccact agtatataaa taccattatt cttcaatcaa ccgcttcacg
     8461 attgcataat ccccttcata aggttcatct acaccattga ttttttgata gggatccacg
     8521 ccttttcccg caataacgac tgaatcttgc ggagcgcttt ctgctaaagc gcgtcgaatc
     8581 gcttcaggac gatccatttc ataatgaacg atcaaatctg gattagtaat tgcttcattg
     8641 atttcttccg cgattttttt cggatcttca aatgctgggt catctgccgt caaaaaggcg
     8701 atgtcagctg tttctgatag cacttttcca aaatcatgtc gacgggagat tgctttgttt
     8761 cctgggctgc ctaatacaac aattacacgg ccatctggat gttcattttt ggcaaatttc
     8821 agcaatgttt tcaagcttag ataattatgt gcatagtcca catagacatg cgctccattg
     8881 gattggatca attgatccat tcggcctggt acacgagctt cttttaatcc gtcttgtgca
     8941 tcttgactgg aggcacctgt tagtgctgcg gcaatgatcg cactggctgc attttcttgg
     9001 ttgaatcctc cagccagcaa aatatcataa gcagtatcgc agatagctaa gcgatcttca
     9061 tgagaagata aagtaaaacc gtgagtacct ttatctgaac ggatcacttg ataattgctt
     9121 tcttccgaac gaccataagt gatcgttgga atctggaata gatcggctgt ttccctaagt
     9181 aaatcaaaat agtcactttc atgatttaaa acaactgttt ttgaattcaa gatcaattgt
     9241 cgcttgcaat agaaatagtc atcaaatgtc ggatgttcga tcggactgat atggtcaggc
     9301 gagatattca agaagattcc gacatctaaa gtcaagccgt atacgcgttg tgttttgtaa
     9361 gcttgtgaag aaacttccat cactagatga gtcataccat tttcaactgc ttctgacatc
     9421 atacgatata aatctaatga ctcaggcgtt gtcaaatggg atttgaaata tgttttgcca
     9481 tctaacgttg tattcatagt agaaagcaat gctgtttttt tatttgttgt gtgatccaag
     9541 attgcttttg taaagtacgc agcagttgtt ttaccttttg ttccagtgaa tccgatcact
     9601 ttcaatttat tttgcggata gtcgtaaaaa gccatagcaa gaagcgccat tgctttacgg
     9661 atatccgtta cgatgattcc tactgctgaa ttttcgtatg gttgttctga tacataatat
     9721 tgaataccct gttcgattgc tgaatccaaa tattcttctt tgaaattcat tcctttacag
     9781 aaaaacaaag tagaagcatc agcttttctt gaatcataac ttaactgaag aaattcagct
     9841 ttttctggta catcaagatg ccagcctctt gttgaaacaa attcttttaa tagattttct
     9901 tttaatagta aatgacggat gtcttctaaa gagaagctca ttgattgatc cctcgcttta
     9961 ttcaatttct tggtttatta tagcacactc tttttaatat atcgtttgtt tccgctatga
    10021 caaaatccag ctttattcaa attttcttga cacaggggac aagcggatga gtattttcat
    10081 aaacaatgaa ctgactgtaa tagataggag aacgaatagt gtctccaaca atccaaaaac
    10141 ataataattc aaaaagacga taagactatt ggtaagtaaa agtccttgcg gcattggaag
    10201 ccgaaaaaat ttgtggaaaa taatcggcgg tatcgttata ccgccatttg aggccccgac
    10261 tgtgtacaat gtgtaaaccc caagactgaa caaaagactt cctgaaatca gtgaaaagaa
    10321 atgtgaggag atcatttcca cttttggaac aatctctaaa acaacaggaa aaagcagact
    10381 tcccgctaca gtcttaaaaa atactgattt tcctaatagg acagcggcta gaagcagcat
    10441 cataagattg atgctccaaa cagtaactga gcgttggata gaaaaggccg tttcaactaa
    10501 atgtccgatg gcgcccactc cggcggaagc tatatcgtga gggagcaaga accaattcaa
    10561 tccaactgtg ataaagaaaa gacctaaaaa tataacgggt agttttttta tttcttgtgt
    10621 cattgatatc tcctataaat gttagccata aaaggctgta tatttcttta ctttaccaaa
    10681 aaaacgaaac cgaattcaaa aaaacaattg ttcttataaa agttatataa aatcgattgc
    10741 cagtttctgt tttaccaatt aaaatgatag taatgttttg tagaaagttg gaatctgacc
    10801 gatggataat caaagaaaac cagcattaga tcaattaaca ccgcaagaaa aaatggcacg
    10861 aggctctgct tggatgaccg ccagtaatat catttcaaga ttgctaggcg ctgtctatat
    10921 cattccctgg tatgcttgga tgggcgaaaa tgctaaggct gccaatggat tattcaatat
    10981 gggatacaat atatatgcat tgtttctact gatctctaca gcaggaatac ctgctgcgat
    11041 tgcaaaacaa actgcacgct ataattcatt gaatgaatat ggcacttcac gacgattatt
    11101 tatccgtgca ttacagatga tgggcgggtt aggcctgttg tttgctttgt tcatgtacat
    11161 cgcctctcct tggctagctc atgcttctgg tggcggagaa gaactgattc caacgatgcg
    11221 ttctttgagt atcgctgtat tggtttttcc ttgtatgagt gtgatacgag ggtatttgca
    11281 aggcaatcaa gaaatgatgc cttatgcgct gtcgcagatt gtagaacagg tggcacgtat
    11341 tttttatatg ctattggcta cattcatcat catgaaagtc atggatggtg actatgtgac
    11401 ggcggtcacg caatcaactt tcgcggcatt tatcggtatg ctagcaagta ttcttgtcct
    11461 tttgtatttt ttgaagaaac atcaagcgta tacatcggcg tttattcact acagcgaaaa
    11521 caaaataaat attgcaacga aggaattgtt gttagatacc gttaaagaag cgattccgtt
    11581 tattattgtg ggttcagggg taacgatctt caaattagtc gatcagttca cttttatgaa
    11641 gatcatgtct gcatccaccg agtattccaa tgcacagtta ctggatcttt tttcgatttt
    11701 tagtgccaat ccagataagt tgacgatggt agttattgca ttggcaacat cgatcgcgtc
    11761 tactgggtta ccgttgatta cagaagctgt tacgttgaaa gatcgagtag gtctggcaaa
    11821 attgacaagc ggaaatttac agttgttttc attttttatg tttccggcga catttggtgt
    11881 tatgttactt gcctatccgc taaatacatt attttataca ccggattctc taggaagtaa
    11941 tgtactgatc caagcaagtt ttgtaggact cttcctagga ctatatatgc tagtttccaa
    12001 catgctgcaa ggaatgtttg aaaacaaggc ggcaattaat tatttggtta ttgggtttct
    12061 agtgaaactc cttcttcaat acccagccat tcgtttattt gaagtatacg gccctttatt
    12121 ggcgacaatg atcggatttg ctgtgtcttg taccttgatc ttaaagagaa tccatgaagt
    12181 agcccgcttt aaaccaagtt ttgtctggcg caggacattg ctgatcttta ttcttacctt
    12241 gattatgctt ttggcttcgt ggatcacaaa gatgattttt ggtgcttttt taaacgaaga
    12301 tagcaaattc cagtctctat tgttgattct tgtagtagca ggtatagggg ctttagtgta
    12361 tggttattta gctttgaaaa tccgcttggc agaaagacta ctaggaccag gaatagcgcg
    12421 tttacgacat aaattaaaaa taaaataagt gaatcagaag agctagattg atgctctttt
    12481 gaaatggggg aaacgcatgc gtttagataa gtttttagca gatatgggac ttggcagccg
    12541 caaagaagtc aaaggattaa tcaaaaaagg aacgatcgaa gtcaatggac aagtggtgaa
    12601 aagcgataaa gcgcaagtaa atgaattcga agatgcggtt acttatttag gagaaatcat
    12661 tacgtatcaa aaagattttt actacatttt gcataaacct gcaggagtca tttctgctac
    12721 tcaggataat catgatcaaa ccgtaatcga tttattggaa gaccaggatt atcgggaaga
    12781 tctttttcca gtcggccgat tagataaaga cacagagggc ctcttgattt taacgaatga
    12841 cggggtgttt gcccatcagt tactatcacc taaaaaacat gtggagaaag aatatctagc
    12901 tgaggttagc ggtataatga cagaagaaga tatccgttta tttgcagaag gactgattat
    12961 tgatggcgat gagcagacat tgccggcaaa actccaaatt gatcaagtag atcaagagac
    13021 agaaacttca attatccgtc tgattttgca tgaaggaaag tttcaccaag ttaagcgaat
    13081 ggtcaaagca gtaggaaaag aagtcttata tttaaaaaga atccgtatgg gaaaattttg
    13141 gctgcctaaa gatttgaata aagggcaata tcgacccatg accaaagaag agctagaaca
    13201 agtgagagaa aaataattga atggatagct aaaagaataa gcagagtagt tgtagcgcca
    13261 tctgaaaagc agacttgtcc gtctgacttt cggatggctt ttttgtgaaa tttcggctta
    13321 tttccgtaga agctacttct ggaacaccgt ttgttcggaa taagggactg tgacaagact
    13381 tttgttacag tccccattgt tccttgtgac tcagagttag atagtaagga cacgatctcc
    13441 ctaatactca ggccaaaacg ctgaattcac aacctctgat aaacggtgtt caatcagctg
    13501 accctcggta ttagctcaaa aaacggcaaa atatgaggaa ctattttccc gtttttttct
    13561 ctaatactca ggccaaaacg ctgatttcac aacctctcct atactttgtt tcattttccc
    13621 tatactttgt ttatttattc tcattattga aagcgctata attacgttgc gaatgaatcg
    13681 aaaggggttt ttagatgaaa aaacgaaagg ggttcaatca tttttttatg aatctatggc
    13741 gttataaagc acttgtcctg atggctgtac cgggtatgct ttggatgatt tttttctttt
    13801 acatacctgt aatggcgaat gtcgtagcat tcaaaaattt tcatatttca gcagatgggt
    13861 tcttggctag cttgagggaa agtccttggg tgggttggga aaatttcaaa tttttatttt
    13921 cttcggatca agcatttctc atcactaaaa atacaatttt atataatgta gcatttatcc
    13981 ttttgaatct gctgatctca gtcgtatttg caatcattat gagcgagttg agaaataaac
    14041 gactcgttaa agtttatcaa acgatgtcct tactacctta ttttctatct tgggtcatta
    14101 tcagttactt tgtttacgct tttttaagtc ctgacaaagg aatattcaat cagtggatca
    14161 ctggacatgg aggagaagct gtcaattggt ataatgaacc gaaatattgg ccgtttatct
    14221 tggtgttcat tggtacatgg aaagggattg gttataacag cattatttac tttgcatctg
    14281 ttatgggaat cgatccgact tattatgaag ctgctatggt tgatggagct agcaaatggc
    14341 agcagatcaa acatgtgacg ataccacaac ttattccact aatgacgatt ttaacgattt
    14401 tagccgtagg aaacattttc cgagctgatt ttggcctgtt ttataacatt cctaggaatt
    14461 ccggtgcatt atatgatgtg actcaagttt tggatacgta tatctacaat ggcctgacat
    14521 ccacaggaga ttttggtatg acggctgctg caggacttta ccaatcagtt gtcggctttg
    14581 tcttgttgat gatcacaaat acgatcgccc gacgttttga tagcgattct gcattattct
    14641 aaggaggaaa aaaatgaaaa aaaagaaagt ggagagagtc caagtccgct ctttcaataa
    14701 gacggctaat tttttctcta atataatgat tgcactcttt gctatttcct gtattttgcc
    14761 atttatattt gttatcgcca tctcttttac cgatgagtcc atattgtcga ctcacgggta
    14821 cagtttttgg ccagaaaagt tcagtatatt tggttatacg tacttgttcg accagatgca
    14881 ggataaaatt ttccaagcgc ttttcgtcac tgtacttgtt actgttttag gtacactgat
    14941 caatagcacg gctacttctt tatatgctta tgcgatttca cggtcaaact ttccattccg
    15001 tcgttttttt accgtttttt gtttgattac gatgttgttc tcaccaggga tggtggccaa
    15061 ttatcttgtt atgacaaatc ttcttcagtt aaaagatacg atttgggcat tgatcttgcc
    15121 aatggctgtc agtcccttca atattattgt gatgcgcaca ttctttaaac gttcggtctc
    15181 tgattcgatt attgaatcgg cacggatcga tggagctagc gaactgcgaa tatttgttca
    15241 gatcgtttta ccgttagctg ttccaggtat tgcgacaatc agtttatttg ccgcattggg
    15301 ttactggaat gactggttca atgctttact atacatccaa gatgataagc tagttccact
    15361 acagtatctt ttgatgaaaa tccaaagtaa tattcaatat ctgacacaaa attcaggagc
    15421 aggaagccaa ttgacaggcg gattagcctc agttccagga gaatctgcca gaatggcgat
    15481 cgtagtcatc tcgacgttgc ctattgcgat cagctatcca tttttccaga agcattttgt
    15541 caaaggtcta acaatcggcg gagtaaaaga ataaagaaac aaaaatataa atcataaaat
    15601 taggaggaag aaaaatgaag ttatggaaga aactagcctt tacaggtgtt tcagcgttgt
    15661 tgttgggaac attagcagca tgcggaggtt ctggttctaa agaccaggca gaagctagca
    15721 attcggacac tttgcagatg tatcaaatcg gagataaacc agataatttc gatcaattga
    15781 tggaagtagc aaacaaacga atcgaaaaag agatcggtgt aaaagtcaat atcaattaca
    15841 ttggctgggg cgattacgaa aagaaaatga acgtcatcat ttcttccggg gaaaattatg
    15901 atatcgcctt tgcgaacaac tatgtttcca atgcccaaaa aggagccttt gctgatttga
    15961 ctgaattagc gcctaagtac gctgaaaaag cttataatga tttagatgaa gcatatatta
    16021 aaggaaatct ggtcaatgga aaattatatg ctttcccagt caatggaaat gtctttgctc
    16081 aacaagtact tacttttaac aaagctttac tagataaata cgatctttca attgatggaa
    16141 tccaatctta cgctgatgca gaaaaagtct tgaaggaatt ccatgaaaaa gaaccaaaca
    16201 cggcagcttt tgccattgga caaggcttca aagtccaagg gaactttgat tatcctttag
    16261 ggaatacgtt accatttgct attgatctgg atggagatga ttcgaaaatc atcaaccaat
    16321 acgataatga aacgtatatc ggactgttac gtacaatgca tagctggtac aaacaaggac
    16381 tcattccaag tgacgctgca acgagcaata aggattatcc actagaagga aatacttggc
    16441 taatgcgtgg agaaacacaa ggaccgtatg attatggcga tacaattttg acaaatgcag
    16501 caaaacaaga acttgtctca aaagcaatta ctgttccact aaaatctaca gctcaagcac
    16561 aaatggcaaa ttttgtcgta tcaaatactt caaaaaataa agaaaaatca gtagaacttc
    16621 tagggctgct taacagtgat ccagaattat tgaatgggct tgtttggggg atcgagggag
    16681 aagcatggga aaaagtcgat ggttcagatg ataaaatcaa attgctagat ggttatcagc
    16741 caaatacaca tatgtctgct tggaatacag gaaacaacaa gattctatat acacaagagt
    16801 ctattacgga tgacatgatt gccaaacgtg atcaatcgat tgctgatgca gaaacttcac
    16861 cgattttagg attcagcttc aacactgaca gtgtcaaaac agaattaagt aatatttcta
    16921 atgtcatgaa tcaatattta gatggattga atacagggac tgttgatcca gatgaaacat
    16981 taccgaaatt gaaagacgct ttggacaagg ctggctatga taaggtattg aaagaaatgc
    17041 aaaaacaata tgatgagttc cgacaagaat aataatgaat aaagacaaag gggttgtgac
    17101 aaaagacgtg tcacaacctc ttgttccgaa taaacggtgt tcaaaagcca gtttctcggt
    17161 attaagctaa atatatgtaa aaatgtggaa agccatattc acaaaatttt tcttactact
    17221 cgaaactaag cggctatttc acaaactctt ggtaaacggt gttcaatcag ctaaccctcg
    17281 gtattcgctc acaaaacgac aaaatatgag gaactatttt tccgtttgtt tctctaataa
    17341 tcaggtcaag gtgctgattg cccaaccttt ttttgtataa tgagaaagta taaacttaat
    17401 gtttggagga gaagatatgt tagggaaggt tttggaagag ttatttatcc ggatctgggt
    17461 cattataaaa ctaaccttgt atttctggat atatacattt gccggcggga tcatctttgg
    17521 attaggtgcg gcatggaaaa cagtcaatga actattttat ttgtacggat tcgagtataa
    17581 agaaatcaca ataaaaagag gctggaatat atataaaaga aattttttaa gaggcaatct
    17641 gctatttagt ttatttttat ctggtactgc tttattgagt tacaatttat atttatctgt
    17701 tcaaatcaaa gggctcattt ttttagctat tgattttatt ttactttttg ccttgctctg
    17761 cctatttgct acttatcaat acagtatgat tttagatagt gaatatacga tctctgttcc
    17821 taatctgcta aagctaagtt ttatttcggt tttttccagc ttttcgtctt ttctaaaaat
    17881 aattattggt agcggtataa ttttaggcgt gacttggcaa ttcaaaggat tgatcctgtt
    17941 tggtgtcatt ggtcttttga cggtttggaa tggtacgatg acaacacatt ggcgtgaaga
    18001 attagataaa cagttggaaa gttatgaata agtttcggat tctattttca aaaggaacct
    18061 tactaaatcg tctgttgcgg aaatacgcgg ttatcatgat cggtgtaacg atgactgcta
    18121 ccgttatttt tagtatccat acttgggaac aaaatcaaaa acaggcagaa aacatgacaa
    18181 gtgatgccgt tcaaagcaca agcagaatgc tgaatgataa aacaaccttg agtaggatca
    18241 tcaaaaacca gcttgtaggg aattccgaaa agatcgaaaa tgtgacgacc tacttaacaa
    18301 aacccataga tcagtatctg atgtatgtat acgaacagca aaacagtacc gatgaattag
    18361 tttctttccc aaatcaaatc aaagatctct acgcaaatta tgaagaacta agtgcgatat
    18421 atatcgtgtt gaatcaacta ccggagtatt atgaatcttc tagagaaaac aaaggtgggg
    18481 aaataaaaaa ggggacacca aaattactag acgcctttta tatcaagttg cctatcacac
    18541 aaggaggagc tgagtcagga agtattttta ttggttttca aaagaaagaa ctagatatga
    18601 ttttagaaaa tctaacgtca ttcaatggac tctctttata catgatttct ggaacgacaa
    18661 atcgactata cacattccat gataataaaa tagctaaaga aacgtttgaa agacaagaaa
    18721 tgatcatcac agagagtctg aagaaaaatt cacaattgcc gattgacgat ctggaaaaaa
    18781 acaattttgt tcaacaccaa gaactgtctg atgattatcg tatattagct gtactagata
    18841 aaaaaagtgt aaaaatcgaa acgggaaaaa atcttgcccc gctgatcatt ggtatcatta
    18901 ttttagacaa tatactgctt atctttcttt ataaaacatt caaacgatat tcacagcaag
    18961 tatctatcgt catgcaggcc atggatcaaa cagcagcagg gaatttagag acgcggatcg
    19021 acataacaaa cacggaatat gaactgaagg aactttcaat cggtatcaac gaaatgttgg
    19081 atagcatcaa tcagtttgta gaagacatct acaaattaga aatcaaacag caagacgcac
    19141 atatgcgggc gcttcaagca cagatcaatc cacacttttt atataataca ttagaatata
    19201 tccgaatgta tgcaatcagc gaaggaagtg aagaattagc agatgttgtg tatgcgtttt
    19261 ccgctttgct tcgaaacaat acgaatcaag agaaaacgat cactttaaaa gaagaactgg
    19321 acttttgcga aaaatatgtc tatttgtatc aaatgcgtta tcctaatcga gtagcctatc
    19381 attttatgat cgatcctgat ttggaaaaga tcgaagtacc taaatttgtg atccagccat
    19441 tagttgaaaa ttattttaaa cacggtattg attttactcg attcgataat gcgctaagtg
    19501 tcaaagtctt gcaggaaggc aaacgggtaa ggatcatcat caaagataat ggaaaaggga
    19561 tgactgaaaa acggctaaaa cagattgaag aaaaactgtc tcatcctaaa gtggaacttc
    19621 acggttctat cggtctgcaa aacgtgaatg aacgtttacg tgccagcttc ggcagcagtt
    19681 attatatgtc gcttgagaat aatgagacag gtggactgac cgtttctatt acattcaagg
    19741 agggatgagc gtgtatcaag tgttattggt agatgatgaa tacatgattt tgaatgggtt
    19801 aaaaaagata attgattggc aaagtttagg ctttcaaata gtagcaacag cagagaatgc
    19861 caaagaagga ctagctgtac ttgaacagcg acagattgat ttagtagtaa cggatgtgac
    19921 aatgccagaa ataaatggac tggaatttat tgaagcggct caaaaagaac gtcataattt
    19981 tgaatttatg attttatcag gataccaaga attcgattat ttgaaaggag gtatgcaatt
    20041 aggcgccgtg aattatttga tgaaacccgt gaataagttc gaattggttg aaagcctcaa
    20101 gaaaatcaaa acacggttag atcagcaaaa tgaacaaaaa aatcaacaag aaatctatca
    20161 agaactgtta ttcagtcaat ggttgaatga agaattggat gaaataagtg aagaagaaat
    20221 aataggacaa ataagtgaga agcagcatag gatagtattg attcaactgg caagaactca
    20281 tgatactctt atcaatagat ggttgcaaga acggcaacaa atcttttact atcaacgaaa
    20341 ttacggagac cgtgtattga tcacgttgtt gttggaggca gatgaagttg atggcttctg
    20401 ttgttttgtt caagaaaact ttcctgatca ggaatggctg atcagtatcg gggaagagac
    20461 agattctttg gacaaaatcc ctgaaacatt ccaacaagcg aaagacaatc tacagttgca
    20521 tcaattttat ggagataaag acgagcatgt tttttacgca gtacaagcag aaacaaaaga
    20581 tcaaaccatc gatttttcaa gtttcagacg cgtgctgcaa aataaacaat tggaagtcgc
    20641 tcaaaaaatg atcactgatt tttttgaaca gtttcagtta gcagccatga tgccggaaga
    20701 tatacgatat tccagttttt tactgtttat ggagattcaa cgggaattga ttgatctgga
    20761 agatgaggaa tatttacaag gaatcgaaaa aatccatcag gcaaaaacgg tgcaagaact
    20821 tcagcagcta cttctgtcat tcatcaaaaa gcatcagaga cagaaaaagt acagtgacaa
    20881 tgtggaaaaa gtaatcgtaa tcctgcacca gcattatcaa gaaccactta cactcaaaga
    20941 agtgtcagaa aacctgcatt taaatgtcat gtatctagga cagctgttca aaaaagaaac
    21001 gaaaaaaagt ttttcagctt atttgaacca tttacgtatg gaaaaagcga aacaactgct
    21061 tttgcacagt aatcaaaaca tcaatgagat agcaagtgag attggctaca acaacaccac
    21121 gtatttctct aaattattca agaaaatcgt cggcagatcc cctaaagagt accgagaaaa
    21181 cagtggagaa tcataaaaga aaaagccaat gtaatgaaat aacttataaa aaattgtcta
    21241 gaaatctaat tttgggctgt tcttattact tgaggcaagg cagctttttc atgacctctc
    21301 gataaacgga gttcaaaagc cagtttctcg gtattaagcc aaatttatgc aaaaatgtgg
    21361 aaagccattt tcacaaaatt tttcttaata ctcgaaacta aacagctttt tcacgacctc
    21421 tccactaaac ggtgttcaat cagctgaccc tcggtattag ctcaaaaaat ggcaaaatat
    21481 gaggaactat tttgccattt gtctctctaa tactcaggtc agaacgctga tttcacaacc
    21541 tctcaataaa cggtgttcaa aagcttgcat ctgcggtaat aagaccaacc aaacgaaaaa
    21601 tatgaggatc tatttttgct tggctgttct tattacttga tgcaggacag ctttttcaca
    21661 acctcttgtt aattatagtt tgataaggaa aaaggcaaat cttttgcttg atatttctta
    21721 gggtgaggga tgatccctaa ttggtaagag atcgtgccgc ctttcagcaa atcttgatgg
    21781 gtaaaatata atttcgtata ttctttgtcc atccgtttga ttttatggat aaattgttgc
    21841 tgcggccgat taggcgttgc ttcaatcgtc aattcctttc catttgacaa aagcaaaaca
    21901 gccttgtcaa ttaatggcat accgataaca tattcaccgg ttccaggcgt gacaggataa
    21961 aatcccagac tattgaagat ataccaagcg gccatacttc cattgtcctc atctccaggg
    22021 taacccattg gagaatcgtc gaaggtctga gtcatcaact gtttaagcag tggctgtgcc
    22081 atttcaggtt gtcttatata actgaacaag taaggatagt gaaaacttgg ctggtttgaa
    22141 atagctaatt gtccaaattc tatcgcggcc atctcactca tttcgtggat ctcaaaacca
    22201 tatcctcctg ggtcaaacgt tgggggctga ttgcacagct caatgagttt ttctttatat
    22261 ctctcactgc caccatattt tccgatcagt cctgaaaaat cgtgataaac agcaaagctg
    22321 ctttgccaag cacttccttc tgcataatct tgtccccaac gattagctga aaatggttct
    22381 ctgaacttgc catctttatc ttttgaacgc ataaatcctg actcatagtc aaagatattt
    22441 tgatagttca atgattgctg ctgatatttc ttcgtcattt cctcttcttc tagtaccttg
    22501 gcaacttggc taatacaaaa atcgctataa gcatagtcta atgtgtgatt gactgattcg
    22561 tggtagtgat taggaacata gccatatgtc aagtaatctt ctgttccttg gcgtccataa
    22621 tttggatgtt tgctttgaac cgtagcacct ttttccattg ccgttagaaa ttcaggcatc
    22681 aagtctcgtc ggatatcttt tacagcagca tctgcaatga ccgcatcgat caatgtccca
    22741 ggcataagtc cacgttcatc aggagacagc cattttggca aatagccact ttctctatag
    22801 ctgttcaaaa aaccttccag catttcttcg tatttttcta cagcaatcaa agaataaaga
    22861 ggataaaccg ttttatacgt atcccaaaac ccattatttg tatagagaac accaggtttt
    22921 atagatcgac tagtcgtatc atagtggatt ttttgcccac taggatcaat ttcataaaat
    22981 gtttgtggaa ataaaaacac ccgatataaa ttatgataaa acgtccgcaa ttgttgttga
    23041 tcatggtgtg tgagctgtat acgatcaaaa taggactgcc attgttcttg actgtgagca
    23101 aggtaagtct ctttgctttg gttttgttct agttccaggt tataagctgc ttgttccgag
    23161 ctgataaaag atgtcccaaa tcggatagtt tgtttttttg tctgttcaaa atacaacggg
    23221 atcgagtcgc tatttttgtt ggaaacagct aactcatcaa ttgttaaagg atattcaaat
    23281 ctcaatataa aataaaaaga aaaatcagga tcttctgaac cagcaaaatg tgtgattttt
    23341 ccttccactg tgtaaggatc tataacaaag agctgatggc gtcccggaaa ggataaaagt
    23401 aaaccgctgt catttttgct gtaatcaata gataagattc cgccatacat gctaggtatc
    23461 aatgtagaac agatgccata acgtaattga cagatcgaca gatgagtcgg gcaaaatata
    23521 ctttcttctg ggcggtaaga actttgagca tggaaaaggg tgttctcctg tagttttcca
    23581 ttgatcggcg taaacagcat atgactgaaa tcacccatcc aaggacttgg ttgatgggta
    23641 agacggtagc cttgaaaaac gcggtcatca ggatgaaacc accagctgcc gttttgatcc
    23701 gtagtttgcg gtgcaaaata attcatccca aaaggaacgc cagtataagg ctggcagttc
    23761 ccattagaga aatttggctg attcgctgtt gcatgtctag tatctatttg accgatattc
    23821 attttatttc tcctttgttg attgtctttt gatgaatgaa actggaaatt tactatcgga
    23881 tggtcgatct tttgtctcta accaagtgac caaagcatct cccgcaagct tacctaactg
    23941 ttcaaaatct tgtgcgatgg ttgtcagtgg cgggtcaaca agagctgctg cttggatatc
    24001 atcaaatccg ataattgaaa aatcttcagg gatatgataa ccatgctgtc tcaagagatt
    24061 catggtctgg atagcgacaa catcgttttc acaaacaaaa gcagttactt catacttagt
    24121 gatataagaa agcaattcat ctaatgtcgc gcttttgtca tccagtgaag tgtggaaagt
    24181 caaaccagct ttgtccaatg cttcaaggta accaaggtaa cgctgtcgaa cggattgtgg
    24241 atgagtctgc gtaccaaaca aataagcgat ttttgaatgt ccctgttcga tcaaataaga
    24301 ggcagcaagc gcaccgcctt ggaaattatc ggaaagaacg gtaggaaaag gtaactcata
    24361 gattttttta tctaatatga cgacagggaa ttctttcaat gaaagctcaa aaagtaagtc
    24421 aagatgttgt tctgtatcaa gagcataata aatcaatccg tcaaattcat tgattagctc
    24481 ttttgctgtt ttattttgta gaaagtcaag cgtcgtcatc atgacatcaa tctgtttatc
    24541 ttgcatcaca gggttcaatc ccttgctaaa atcaccaaca gacaaatcag cgacatatgg
    24601 aagaagaaac agaatccgtc gtgtttttgt aggagaagca agaatctgtt ccggagaagc
    24661 ggatttaacg aaactgcctt tcccgcgaac gcgataaata agtcctaatt gctctaattc
    24721 agtcaatgca cgtttggaag tgattctgct tactttatag gtacgtgaaa gttctttttc
    24781 agttggtagt tggctgtcta caggtaattc accagttaaa atagccgttt gcaaatcatt
    24841 aaatattgtc tggtacaatg gttgattcat ataaa
//
LOCUS       DAXWOO010000024        24579 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.024, whole genome shotgun sequence.
ACCESSION   DAXWOO010000024 DAXWOO010000000
VERSION     DAXWOO010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24579
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.024"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(153..551)
                     /locus_tag="ACGE70_000788"
     CDS             complement(153..551)
                     /locus_tag="ACGE70_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFF8692442.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(548..1321)
                     /locus_tag="ACGE70_000789"
     CDS             complement(548..1321)
                     /locus_tag="ACGE70_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFF8692443.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1318..2001)
                     /locus_tag="ACGE70_000790"
     CDS             complement(1318..2001)
                     /locus_tag="ACGE70_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFF8692444.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2168..3055)
                     /locus_tag="ACGE70_000791"
     CDS             complement(2168..3055)
                     /locus_tag="ACGE70_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8692445.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3052..3921)
                     /locus_tag="ACGE70_000792"
     CDS             complement(3052..3921)
                     /locus_tag="ACGE70_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8692446.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4058..5344)
                     /locus_tag="ACGE70_000793"
     CDS             complement(4058..5344)
                     /locus_tag="ACGE70_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFF8692447.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5617..6195
                     /locus_tag="ACGE70_000794"
     CDS             5617..6195
                     /locus_tag="ACGE70_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFF8692448.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6195..7124
                     /locus_tag="ACGE70_000795"
     CDS             6195..7124
                     /locus_tag="ACGE70_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8692449.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7121..8857
                     /locus_tag="ACGE70_000796"
     CDS             7121..8857
                     /locus_tag="ACGE70_000796"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFF8692450.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8920..9756)
                     /gene="pheA"
                     /locus_tag="ACGE70_000797"
     CDS             complement(8920..9756)
                     /gene="pheA"
                     /locus_tag="ACGE70_000797"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFF8692451.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9753..10262)
                     /locus_tag="ACGE70_000798"
     CDS             complement(9753..10262)
                     /locus_tag="ACGE70_000798"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFF8692452.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10255..11550)
                     /gene="aroA"
                     /locus_tag="ACGE70_000799"
     CDS             complement(10255..11550)
                     /gene="aroA"
                     /locus_tag="ACGE70_000799"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFF8692453.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11563..12654)
                     /locus_tag="ACGE70_000800"
     CDS             complement(11563..12654)
                     /locus_tag="ACGE70_000800"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFF8692454.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12671..13837)
                     /gene="aroC"
                     /locus_tag="ACGE70_000801"
     CDS             complement(12671..13837)
                     /gene="aroC"
                     /locus_tag="ACGE70_000801"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFF8692455.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13839..14903)
                     /gene="aroB"
                     /locus_tag="ACGE70_000802"
     CDS             complement(13839..14903)
                     /gene="aroB"
                     /locus_tag="ACGE70_000802"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFF8692456.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14936..15958)
                     /gene="aroF"
                     /locus_tag="ACGE70_000803"
     CDS             complement(14936..15958)
                     /gene="aroF"
                     /locus_tag="ACGE70_000803"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFF8692457.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16617..17591)
                     /locus_tag="ACGE70_000804"
     CDS             complement(16617..17591)
                     /locus_tag="ACGE70_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFF8692458.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17572..19455)
                     /locus_tag="ACGE70_000805"
     CDS             complement(17572..19455)
                     /locus_tag="ACGE70_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFF8692459.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19473..21698)
                     /locus_tag="ACGE70_000806"
     CDS             complement(19473..21698)
                     /locus_tag="ACGE70_000806"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFF8692460.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            21813..22847
                     /locus_tag="ACGE70_000807"
     CDS             21813..22847
                     /locus_tag="ACGE70_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8692461.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(22914..23927)
                     /locus_tag="ACGE70_000808"
     CDS             complement(22914..23927)
                     /locus_tag="ACGE70_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFF8692462.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24005..24193)
                     /locus_tag="ACGE70_000809"
     CDS             complement(24005..24193)
                     /locus_tag="ACGE70_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFF8692463.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
BASE COUNT         7065 a         5341 c         4397 g         7776 t
ORIGIN      
        1 agcatatttt gaaaagaaag tctgatgctt agagtagaat ttcataaaaa tttactattt
       61 cataagtatt cacgagaaaa atacaaacaa aaaagcggaa ataacgctca tccgccttct
      121 tgtttgtgtt tatttcctct tcaaacgctt gtctatgatt cagacttttc tctatcgtta
      181 ttttcatcta aaagttcttg cattccgata atttctcgat attgatgaat cttagtgata
      241 ccaatgactt tgtctacatc aatactaaaa caaataccat tacctggaat atcgatgttg
      301 atgccttgac caatcgcatc cattacttta ttcgtcaaac tatcgggtac taatgtcaac
      361 acaatatctt tttctggcga aatgtccaaa cctaaaaatt tccctgaatc atgtacacca
      421 gaacctcttc catgtaagat cgttccgcct gctgcacctg cttcttttga aaagtcgata
      481 acatcttcgc ccattcctcc gtcaacgatc gtcacaatca tgtgaagatt caatgctaca
      541 agatctttca tttgctttcc cccaattttt gcaatctttc tttttcgatt ctgtcttttt
      601 catcttttcg tttataaata tatcctagta ctaaagtgga taatattggt gtcaatgcaa
      661 tcattgcaac cacaccaaac ccgtcaatga tcggatctct tccttcaatt tgtgcagcga
      721 ttgccacgct cattgataaa ataaatgtcg aacacatcgg gcccgttgct actccaccgt
      781 tatcaaaagc cattgctaaa aagatcttgt ccacttttcg tcctaaaata aatgctagcc
      841 cgtatcctgg gatcaagaaa taataaagag agaatccgac gagcaaacgc cacatagaca
      901 atcctactgc tgcgccgatc ccaattgaaa ttaccatcag catgattttc gatcggattc
      961 tcccctcgct caattcttca acctgtttga tcattacatg aatcgccggc tcagcaaacc
     1021 ctactaagaa ccccattaaa aagccaaggg gaaccagtat ataattgtaa ggcaatcccg
     1081 caatagcact gcctaaatgc tgtcctaccg gtacataagc catattgact ccgtgcagga
     1141 aaaggatcaa cccaatagtc gttatcaaga aacctttcat aatgtcaaag aaacgctttt
     1201 tgcgcatacg aaaactaatg atattcatta aaacaaatag gattacgata ggtgccactg
     1261 ccaaaatgac ttccatggta acgatttcga ttccatctat cacttgctgc cagaaactca
     1321 ttggaagatc acccccaaaa tcatcacggc aagaatcggc cctaacgaag caataccgac
     1381 cattccaaaa ctgtcatttt catttttctt ttgtttgatc atactagtca tccctccggc
     1441 aagggccaag ataaatggaa cagttactgg accagtagtc actccccccg aatcaaaagc
     1501 aatcggcata aattcggaac tggtgaaaaa tgaggcgatc aaaacgccta tgtagcctat
     1561 agccatcaat tgataatagg atagcttgaa tacgacccga agcagcgcaa aggctagaaa
     1621 tactccagtg ccgacgctga cgatcccaat cagcaacact ctgccaatct ctccgtcaga
     1681 tatatcatat acttgttgtc ccaaaacttg cacggaaggt tcagcaatcg tgatcacgat
     1741 cccgatagca aatcccaaac tgaccaaaac ggtcaaactt ttctttttga tcatatattt
     1801 ccctactaga tctccgactt ccatcataga ataatctgct ccaaacaaaa agagcgtcat
     1861 tccgatcatc atgattgccg caccaaccag aaatgaagcg aggtcttctc cttctaacgg
     1921 tgcaaaaaca aacgttaaaa tgacaatcag gacagtcatc ggcaatatag cagcaatcac
     1981 ttctttaaaa ttttttagca tgtatcctcc tcctttactc tttaagttta ccttaaaatg
     2041 aaagagtttt cgagcacaaa cttaaaacgt tatcatttaa tttttctcaa cataaaataa
     2101 cacacgtccc aaaagtatct ttttacttgg aaaaatgact ttgaaggaac gcgcttattg
     2161 tgttttttta ttttacagag ccgatcattc cttgaacgaa ttgcttttgc aaaacaaaaa
     2221 atacgatggc agttggtaaa gtagcgatga ctaatccagc catgatcatt ccatagtctg
     2281 gtgtatagga agcacccata gcagataaaa caagcggaac cgtccgttta tcaggagatt
     2341 gcaaagctac taagggccat aaataattgt tccagctact catgaatgtg atgattgcag
     2401 ccgcggcata ggtattttta gccgtcggca tatacactct aaaaaaaata cttagttctt
     2461 ttagaccgtc taatcgagcg gcttctacta gatcttttgg aaaagattta acattttgtc
     2521 gaaagaagaa gatcaaaaaa gcagtgctaa cagatggcag gatgacaacc aagaaactgt
     2581 tgattcccaa aaccgttccg ttcaattgtg aaaacattcg atataaaggg atcatcaatg
     2641 ccgcaaaagg aaccatcatc gatagcagta gaatattgaa aattaggtct tttcgtttac
     2701 tttgataaat ctcaaatcca tatcctgcca ttgacgaaat cagcaatgca aacacagtgg
     2761 tcaccaaagc gatcatcgct gaattccaaa gggaacgagt aaaattcagc tcattggaaa
     2821 acaaattttg aaaattgatg atcaagttac tgccaatctt tatttttccc gatgtgatat
     2881 ccacgggtgt attcgtcatt cccaaaacca tccagacaaa agggaaaatg gaaataaatg
     2941 ataggatgct taatgctgta tactttaata ctgacccaat tgtcgaacga agttctaatg
     3001 cttgtttttt gacgttgggg cgttcgattg tttctttttc taccatattt ttcatcgact
     3061 ttctcctcct actttcgttt ggattatcga caatatcaca ataaaggaaa caataacaaa
     3121 ggaaactgct gccgcatatc caaaattagg cgtgaattta taactcaaat tatagatgta
     3181 ttgagatagg gtggtcgttg catttgctgg tcccccgcca gtaatatttt gtacttcatc
     3241 aaaaagctgc aatgtcccga tcgtcgaagt aatggaagta aacaaaatga ttggttttaa
     3301 atttgggatc gtcactttga taaactgctg ccatttagaa gcaccgtcaa tagatgctgc
     3361 ttcataaatc tccgaatcaa tattttgcat gcctgaaagg aaaaaaatca tattgtatcc
     3421 agtccaacgc caagtgatcg aaagaatgat cagaactctc gcccaaattg gatgagtcaa
     3481 ccagccaatc ggtgtgtcaa tcataccaat gcccaacaac aagttgttta caaggccaga
     3541 ttgagaaaat agacttttca tgatcagaga ataactgacc atcgaagtga tacatggaag
     3601 aaagatagcg gttcgaaaca accctttata tttcaacttt ttatcattta aaaggttgga
     3661 aataaaaagt gctagaaaca acatgatcgg tacttgtaca agtaaataca ggaatgtatt
     3721 aaaaaaagct tttttaaaag ttgcatctgt caacatccgc tgataattac ctaacccgtt
     3781 aaatgtcata ttgacagggg gaccactttg aaaagacgtg atcaaagcca aaaccatcgg
     3841 aataaaaacc atgattgata tcaaaatgac tggcaagatt aaaaaagcat gcccgttttt
     3901 gaataaatct ttctgcttca ttcttttccc tccaattcta tcattgatta tctacatgaa
     3961 aagacaagaa agacaatatc tgtaaggcag acgtgatctt ttttgtcttt tcgtttgtct
     4021 gatgaaaaag cgaagaaatt cactttttta ccaccgatca attagccaat tgtgcttcta
     4081 cttgtttttg tgtatctgct agtaccttgt ctgtctcttc tccattgatg attcgatgaa
     4141 gagcttcagc tacgactgct tcaattgcat aggtctcatg tccgtaattc acttctggta
     4201 tttcagatgt ccatgttgaa aaatcgtcaa agatcttttg accactgtaa aattcagaag
     4261 cttcttgata agcttcttgt tcattcgcgc taagcatcgt agatactagt ccgatttctt
     4321 tcgcaagagt gcccatcagt tcttcattcg aagcaaacgt tgcttctagg aaatctttcg
     4381 ctgaatcttc tccagctact cctttgatca cataccagcc agcgccgcca aggttagaag
     4441 cttgcgcttt ttgcagattt tctggaagtg caggtattgg tgcaatcttc cattttccag
     4501 attggttttc tgctccttgg atcgttgaag aataccaagc gccagttggg ctagaagcta
     4561 ctgctcctga ttgtatcgca gttactccag cattccagtc agaggtttgt tcgactagat
     4621 cttctttcaa aagagtggca aacaattcaa gaccatactt taaagatgca ttgtcttgga
     4681 tcgtgactgt ctccccatct tcagatgtat accattctcc tgcctgttgc ataatcatcc
     4741 ttactcgacc aaggtcagaa ggattgactt cagcgatttt ctttcccgtc ttttctttga
     4801 catctcgcgc tacttgaata tactcgtccc acttcagatt ctccatatcc tcttctgtat
     4861 aacctgcttc tgccatcaga tccgtacgat agaagtttgc tgttacgccc gaatcgaaag
     4921 gaacaccgta tattttatct cctactttgt ttaccgcaaa tttatacggt gcaaaatgat
     4981 cttcctttac aatagcagac aaattagcga atgcatctgg ataagaagtc aaatagcctt
     5041 gtatcctata atcttcaatc aagacgatat tgggcaatcc ttctgtattc ccgctcgcta
     5101 aagcggtgtt cagtttctga acgatatcat cctgagacat cgtaacaact tctatctctg
     5161 tttcttcgtt ctcataaact ttttttgctt cattcactgc tttgatattg aatgtctcat
     5221 cccacgccca gacagtgact ttttcttctt tgtctgctgt actactagca cttccgcatc
     5281 ctgttaagaa acctgatgct gccgctaaac aaacgactcc agcaattaac tttctgatgt
     5341 tcatttttat ccgctccttt tccatccatt agttgtttac gtttgctaca ttcatcatac
     5401 caaaatttga aaacggtttc tttattattc agatacttca aaaatttctc ctaaaataat
     5461 aaaaaaatac aaatcaacta tgacaaaagg aataatctta gcatctagac caaagatcga
     5521 aatggtgagg tttctttaaa ataaaaatat ccttgcattt tctcctagaa ataagtattt
     5581 ttaaattaca agactattct taaggcgggt aaaaaaatgc caatacgcgt aattcttatc
     5641 gatgatgaac agacgatttt agatggaatg aaacagttga tcaattggtc tgagcacggg
     5701 tttcaattaa tagggacttt ttccagtccg ctgaaagcgc ttgatttttc tttggtacat
     5761 gcaatcgata tcgtggtcac agatgtaatg atgcctgaat taaatggaat cgaattatcg
     5821 cggctattaa aacagcaaca acctgaaaca cagatcctta tcctctctag ctatgatgaa
     5881 tttgatatgg ttaaagattc ttttaaagaa ggcgtcgctg attatctttt aaagcctcga
     5941 ttgagtcctg atttttttct aaattcctta actgccgttt cacaaaaaat caaaaaaaag
     6001 cccaacaaaa gcccaatcac caatcgttat gagcagatct gcgagaatct ttcacagcag
     6061 attgctggac aaactcgtcc attgcttgac cctcttcttt ttgaacacac cttgtttttc
     6121 tgttatatac cgaaaaacgg ccattcagtt caaaacaagg ctctaaacaa aaacagtcga
     6181 tcacccattc attgatggat agcgacgatt cgtttactat ttatcctgtt tctacctttt
     6241 cggaagaatc cggctatgta atcaatactg attccaaaga tacacttagg aagtttgttc
     6301 aaaagtttcg tcaagaagtt tttccaggtg atttttttgt tgtttctgat tcttttgcaa
     6361 tccaagatat caatgaaata ttccaagaga tcagaaattg caccaaagga caacgtttct
     6421 atcataaggg aaagaacctt gcttatcagc atgaacttct tccgttatta accaaccacg
     6481 agcagcaagc aagtagttat ttaacaaaaa tcgttcataa agactacgct ttagctgtca
     6541 aagaacttga gaatttcttc acgacgatat tggaacagcc tattgatcca gcttatttga
     6601 aacatgaggc aatcaatcgt ttctatgcat tactcaacgc tttggctgac gagcatgttg
     6661 ggaatgaaga actttctttg ctcaagatcg cccttccttc tttattagcc gatgctgagt
     6721 atttcgaaga tttttctatt cttgtcaaag aaacacttga gaaactcacc cacttttctg
     6781 caaatgcaaa aaaagacaat ttcgatatct tgactttgat ctacgagttc gtccaggaaa
     6841 attatgagaa agaaatcaat ttacaacttt tatccgaaaa atatcatttt tcatatagct
     6901 atctctcagc catctttact gaaaaatttg gtatcaactt ttcaaaatat ctaaaaaaag
     6961 tccgtatcag taaagccaag ctctttttga cggaaacacg gcttactttg acggaaatct
     7021 gtgagaaagt cggctatacc gaactgggtt atttctccag agtttttaaa gaagagacag
     7081 gaatgacacc ttctcaatac cgtaaaggaa cttttgtaaa atgagaaaaa aaatcaaaga
     7141 gcatgaactc tttgccaaac ttctgttcat tttgttcatc ggtctttttc tgcaagccat
     7201 cgtcatttct ttgtttattt atcatcgatc aagggatgcc tatatccagc tgttcaatca
     7261 atcgaatgat gtggtactaa aaaagattca atctgaattt gaaagtttga atgatacaat
     7321 cgagaataca ttagccgctt ttgattctaa tccagctgtc aaaagctatt tttccaatga
     7381 tccagcccaa catatggaac agcttcagca actaagaaca atccaaaaaa tgaatgactc
     7441 tctttcaaaa atccatccaa tgatcgatta cgatgtattg atctttggag aaaatggaag
     7501 aacttttgtc ggaaacgaca tgctcactgc cgtatctgcc gattcttttt ttcaatctgc
     7561 gattgctcag agagtgaatg aacgtgctgc tgatacacaa atgctttttg cacaccacgg
     7621 attgactctt cgcgataaga aagcacccag cgtcttcttt gtcaaaaaac tgaaaaatac
     7681 attgaatcac gtttacggat atgctgtttt atcgatctca tccaagcaac tagcgaatct
     7741 ttttcaatct gtagtaaatc ctgagatatc gaagatctcc ttcatgaacg aagaacagac
     7801 gatcatcgca tcaaatgaag aaaatacaat tgggagccga tcaatgattt tcaaaaaact
     7861 aacagtaggt gaaacaacga tttcaaatgg acagcagttg acccgtcttc ctctgtaccg
     7921 ccaggattgt acattactca gtcaagtcga tcttcattct ttatctaacc aaatgggcgt
     7981 gatttttccg attattttgt ataatatcgt gacacttgct tttgctggct cattggtatt
     8041 tatgtatttg gatcgacaca caaaatcgat ctaccgcttg attgattcgc taaaacggct
     8101 agaggaagat ggcttaaaag ctactgttcc tgtacaagga agccatgaag tccaattgct
     8161 agggaatacg atcaatcaat tgctaagtaa tgctcagcat cattatgaac agacgatcca
     8221 agacgaaaag aagaaacgtt cgcttgaaat ccaaactatg caagcacaga tacagcccca
     8281 ttttatttat aatacgctga ctagcttgaa atttttgatt tggcagaaaa aaacagatga
     8341 agcagttcaa ggaatcgaca gtttcattac tttactaaga agtacgattg ggaaaaaaga
     8401 agaagtgatt ccagtcaagg aagaaatcaa aagcgtacaa agttacatcg atcttttgag
     8461 tatgcgatat ggcgaaagaa tctcggctaa aataatgatt cctgatgaac tgcagtcatt
     8521 atctatgccg aatatgatcc ttcaaccaat tatcgaaaac gcctatcttc atgcttttca
     8581 caataaaacg caaggattca tcacggtgta cggcaaaaca aaaaatcagc ttcttttatt
     8641 cgaagtgatt gataacggcg atggcttcga tactgaaaat accaaacaaa aaaatgatca
     8701 tttttcagga atcggcatca gccatgtaga tgagcggata cgcctaatgt atggcgatgc
     8761 atatggctta acgatccaat cagtgattgg gacaggaacg actgtaacga tttgtctacc
     8821 gcttcttcct aaaaatcaat cagaatatca aaaataaaga atagccaagc gctgattttt
     8881 ttattttaag ttaaataaat caatcagtat tcttgacttt catctacgcg tctcttctac
     8941 tgtcagcact ggataacagc ccaatatttg actttctcct ccgagcatct tgatttcttg
     9001 gagcgcattc tctactaatg tcattggacg gtccaacaac aaatcgatca cgaaaaaata
     9061 ttctcccaag ctagttttca atggtcggga ttcaatctta cttaagttga tttcccgcca
     9121 gccaaaagct gccagcattt tatgaagcat ccctggccga ttagcaggta gtgtcaagat
     9181 gacactcatt tttactggag ctgactgctg gctggtcatc tttcggtcac cgacgatcca
     9241 aaaacgtgtc tggttcaact cattatcttg gatattctca gctaagatct ccaatccaac
     9301 atgctgtgct gtttctaatg aagcaatcgc tgctgcgtct tctttcggat gttctgctac
     9361 atacattgct gcagcagttg ttgattctgt tgctactaaa ggaacattag gataatgcgt
     9421 ttctaaaaac tgttgtgatt gtgctagtgc ttgtggatgt gaaaggattt ttgtaatctt
     9481 tgttgctgga ttgcctaaaa gctgctgctt gataggaaga acgatttcac ttttaacttc
     9541 gacttctgga tgtttggaaa gaagatcgat tgtgtgatga acagctcctt ccaatgaatt
     9601 ctctacagga acgacagcca gatcgacttg tttgcgaaac aaagcgcgca aacaaacagg
     9661 gatggaagca tacgcagtca attgttctgt aggaaatagc tgacaagccg cctgataagt
     9721 gaatgaactt tctggcccta gatagctgac gttcatatgc ttttgatcct ttctataatg
     9781 gtatcgatga tcttttctgg tgtttgattg gtcgtgttga ttcgatgtgt cgcactttct
     9841 tcataccaag aaagtcgttg tgccaataat gcttttattt cttctcttgt ttttccaaga
     9901 gctaaaggtc gaatagacgt ttcatcttgt tggatacgat caactagaca gtccgcttct
     9961 gcctctaagt aaagaacaat cggctgtgtt tttaaaaaac gccgattttc tgcactttgg
    10021 acaatccctc caccagtcgc gataattttg tcttcatttg acagctttct taaaatatct
    10081 gactccactt ttcgaaagct tttttcaccg taatagtgaa aatattcggc aatcgacaat
    10141 tggatatgtt cctcaatctt tgtatctaaa tctacatact ctttttgtaa acattgagct
    10201 aaccctttac cgattgttgt ttttccagcg cccatgaaac caatcagcaa aatgctagcc
    10261 atgagcttct cctcctgaca caagttctgc taaatcgtca aaaaatgccg gataagagat
    10321 tgaaacagct tcagctcctt ctagttcgca aggtccatca gctatcaaag cagctacttg
    10381 cagcatcata ccgattcgat gatcgcctct gctggacact tttgcgccat gcagaggtgt
    10441 cggcccatgg atgatcagac cgtctgctgt cgcttttatc tttgctccca ttttctgtaa
    10501 ttcttctgcc accgcatcaa ttctgttggt ttcttttact tttaattctt ctgcgtcttt
    10561 gatgatcgtg attccttctg cttgagtagc cactaaagct agaatgggca attcatcaat
    10621 cagacgcgga atgatttctc caccaatcgt tgctttcttc aaatgactcg tcttgacact
    10681 taaatcagcc gattggttct gttcattgtg attcgtctgg gtaatcgctg cgcccatctc
    10741 ctccaacaca tctaagatac cagttcttgt tggattgacc ccgacatttt ttaacaacag
    10801 ttggctttcc ggaacaagaa gtcctgctgc tagaaagaaa gcagctgatg aaatatcacc
    10861 tggaacagtt atctgttgac cagttaattt ttgcggtccg gtcactatga tcgttttatc
    10921 ttccactgtg atccttccgc caaattgacg aatcatttct tccgtatggt tacgggaacg
    10981 ttctttttca acgattacgg atgtgccttc tgcttgtaat gccgcaaaca gcaaagcaga
    11041 tttgacttgc gcactggcta caggcatatt gtatcggata ggtgataaag agcgggttcc
    11101 tgtcacagtg attggtagat actggtcatt ttcttctcct tgcaagtcag cgcccatctc
    11161 gcgtaaaggt tccatcactc gccccattgg acgtttagac aacgaagcat ccccgaacag
    11221 agtagtcgta aaaggctgtc cagccaaaat acccatcaat aaacgtatgg tcgtaccgga
    11281 gtttcccatg tcaattgatt cggttggttg gacaaatgag tggctaccgc gaccatgaat
    11341 cttgatcacc tcttcctctt cttcgatctc gacacctaat tgttggaagg catgtaaagt
    11401 actcagacaa tcttctgcgc gcaaaaaatt ttgtatagtc gtcgttcctt cagcaattgc
    11461 tccaaacatg atgctgcgat gggaaattga tttatctgct ggtatcctaa cagtccctct
    11521 taatccatgt atttgttgta ataattgcat ttatccagcc tcctatttga tccagcaatg
    11581 ataactggta ttagacataa tgattctttt tgcttgtaac aaatcctgtt ggtttttaaa
    11641 actgatttgc agtattccta aaatatcttc cctggtctct tggatcttca aattaatcaa
    11701 agaaactccc gcttttccga tcagtccagt tacttctgca ataacgccag gttcatctgg
    11761 cacatcgata aataaatcaa aaaaggcagg aatcgcgcct tgcttatgga caggcaactg
    11821 gtctcgactt tcttttgccc gattgaaaaa agaaaagatt tccgattggt tctcagtcat
    11881 gatccattct gctatttgtg tcatttcttg ttgccaatct gccaatcgtt ccaataatat
    11941 ctgcttattg ctcaacaaaa tatctgtcca catgacagga tctgaggaag cgatcctagt
    12001 gatatctcga aagcctcctg ctgccagttg tttggctcgc ggatactctt gattgaattg
    12061 ttctgcctga ttgactaagc cagctgccag aatatgtgga aaatgactta gcattcctgt
    12121 tatacggtca tgttctcttg ctgtaagagt tacatatttt gctttggttc cgcaaaatag
    12181 ttcttgcagt ttttcaacat cttttttcgt cttttccaaa ggtgtaaaga tgtaataggc
    12241 attttcaaat agtttagcat ctgctgcctg tatacctgat ttatgagaac cagccattgg
    12301 atgtccaccg acaaaccgaa gatttttctg ctcagcggat gtcacgactt gctgcttcgt
    12361 actgctgaca tccgtaatga tgacgtgatc agacaagttg attctttcaa ggacatcaag
    12421 ataagctaag gttgtacgga cgggaacggc taataagata acgtctgctt gttctgcacc
    12481 tgtttcaaaa tcacttgcaa cgtaatcaac gatctgctgt tctactgctt gctcacgggt
    12541 ttttgactga ctatcccaac caactactat cagttctgga ttggtctgac gaatacaaag
    12601 tgccatagaa gcaccaatca gtccaagccc cacgataaaa acatgttgtg ccatcttatt
    12661 tgcccccttt ttagtaatgt ttcaagtagt cacggtactc cttcactgca gctttcaact
    12721 caacaagcga atcacttggg aattttgaca atagctctgt cgctaccaca gtggctacaa
    12781 cattttccgc aacgacgcta gctgcaggaa ctgctgtact atccgaacgt tctacacttg
    12841 ctttgtaagg ttcttttgta tcgatatcaa cactttgaag tggcttgtac aatgtaggaa
    12901 tgggtttcat gaccccgcga atgacaagtg gttcgccatt ggtcattccg ccttcgaagc
    12961 cgcctagatt attggtacgt cggaaatagc ctctttcttc ttcccatagg atctcatcca
    13021 tcacttctga gcctggtaat gaaccagaag tgaatccgac cccaaattct acgcctttaa
    13081 aggcattgat actaaccaca gcttgtgcga ttttggcatc tagcttcgtg tcccattgta
    13141 cgtagcttcc taatccagct ggaacgcctc caacgagcac ttctacaatt cctccgatcg
    13201 tatctccatt tttctttgtc tgatcgatca aatcttttat tctttgctca actgctggat
    13261 caacaactcg tacttctgac gcttcagaaa tttctttgat ttcttgaatc gagatatttc
    13321 caggtatttc tgcttggatt ccaccaagac aaatcacgtg tcctgctacg atgatgtcaa
    13381 gttcttccag aattttcttg gcaattgcgc cgattgctac acgcatggtc gtctctcttg
    13441 cagaggaccg ttcaagcaca ttccgaagat ctcgatgatg gtatttcata ccgcctacca
    13501 aatccgcgtg tcctggacgc ggacgtgcaa ctcgccgcag tgattggtct ttttcaggca
    13561 cttctgctac agacatgaca gttgtccaat ttttccaatc tttattttct accactaaag
    13621 tgacaggtga accaattgtt ttgccgtgac ggatgccaga cgtgatccga acacgatctt
    13681 tttcgatctt catccttcct cctctgccgt aaccagcttg tctgcgagcg agttcgtgat
    13741 tgatttcttc ttctgacaga ggtaatcctg ctggtagtcc ttcaataatg gctgttaatt
    13801 ctggtccatg ggattctccc gcagtcatat aacgcatctt acagtcctcc taagtaatct
    13861 ttgaatgtct ctgtgggaac aggaacgatt tttgcttgcc caatcttttc tagcaagatg
    13921 atattcaatt ggcttcctct tgctttctta tcatgggtta tcgcgtggaa taattcatct
    13981 ggatgactta gatcgtacgt aatcggcaga tggaattttt gaagtatcct ttctaaagca
    14041 gctgtcgatc cagttggaga caagccgatt ttttctgcat gagaagtgat ggctaccatt
    14101 ccaatagcta ctccttctcc atgactgact accccatatc ctgctgtatt ttcaattgca
    14161 tggccaattg tatgaccaaa atttaaaata agtcgatttc cttggtcaaa gacatcttct
    14221 tccactactt ttcgtttgat ttccaacgta gcagtaatga ttggaacagc atgttccaga
    14281 agatcttttt cgtctttcag ctcgttcagc tgtttccaaa gcgttgcatc tgctatagct
    14341 gctgatttga tgatttcagc gattccttct cgtaaacgac gtaccggcaa agtttttaga
    14401 acctcaggat caatcaatac accagctggc tggctaaatg ttccgacaag gtttttcgct
    14461 ttgtttgtat tgactgctgt cttcccgcca atactagaat ctacttgtgc aagtagcgta
    14521 gtcggaattt gaacgaaatg gatcccacgc atgaacgtcg aggccacgaa tgctgctaga
    14581 tcacccacca ttcctccacc aagtgcaagt atcccatcgc tgcgggtaaa atcattatct
    14641 gctaaaaaat cgtataactc agctgcttgt tctaaagatt tactggtctc acccgccggt
    14701 acgattgaaa ggactggttc aaaattagct tgtctcaatt ggaacatgat tttttctgca
    14761 tatagtggag caacgtttgt atcagtgatc acagctactt tttgaggtct ccaaatcgtt
    14821 tgaagccatg atccagcctg ttctagtgcg cctttttcaa tatgaatatc gtatttttgg
    14881 ttatcaacat gtacagtaag cattcgtttc acttctttct tttaaaaagc tggaattaat
    14941 cgagtgtctg aatcttttcc attgcttctt tcatgatcgc aacttctttc atcatgcgtg
    15001 agtaagtctt ttcattcaac gattggggac cgtctgacca agcatttgca ggatcaggat
    15061 ggatctctac gatcatgcca tctgctccgc ttgccacacc cgcacgagcc attggtggta
    15121 caagttccca aattcctgta ccatgacttg gatcaacaat cacagggaaa tgagttaatt
    15181 ttttcatcaa tgggacagca cttaaatcaa acgtattacg tgtcgctgtt tcgtaagtcc
    15241 gaatcccacg ttcgataaaa atgacaggag atttgtcttc gactgcaatg tattctgcag
    15301 cattaagcca ttcattgatc gtaccggaaa ttccacgttt caatccaaca ggtttacctg
    15361 ttttaccgac agcagatagc agtttaaaat tttgcatatt acgtgcgcca atctgtagga
    15421 tatcgctgta ctcagccacc atatcgatat gcccttcatc catcacttca gtaatcactt
    15481 tcattccaaa ttcatcagcc gcttgacgga tgtatttcaa tccttcttct tctaaacctt
    15541 ggaaagcata tggagaagta cgtggtttga aggcaccacc tcgcagaatt tttgcgcccc
    15601 cggctttggc cattcgcgca cattctctga tttggtctaa tccttcgata gaacaaggac
    15661 cagccatcgt cacgaaactg ccgtcaccaa tttttactcc gtctacatcc acgactgtat
    15721 tgccaggatg gaattctcga gaagtcaatt tgtatgtgtt taaaatacgc acagcatttt
    15781 ctactccttc atagcgtgta aatgctcctt cttgtaaact tcgcgtatca ccttttagtc
    15841 caatcactac tcgttctgtt ccttgatcga ttgagacttt gaatccttct ttttcgatat
    15901 gatcgacgac tttttttatt tgttcttccg ttgcttcttt tttcataatt actatcattg
    15961 tttttcccgc tttcttttgt ttacttatct atcttggctg attttctcta ctttttcaag
    16021 gtggttttac attaattcct actaaatgac cgtgtcctgt tgttctcatt ttcgctcctt
    16081 ctttcctact tatgacccag ataatcaaca aaaaaagatg tccctgtagg aaattgcctc
    16141 tacagtgaac atcttcaacg agccttaaaa agcgctgcaa aaatataaaa tagccactat
    16201 agatgaattt ctctacgtgg ctgtaagtcg tgtttaaaaa tctttagcca tcatagatac
    16261 aaacctggtt tgtttgtacc cacgaagtca tgaatacaaa cctatctaaa atagctaaag
    16321 taatacgtat tcagttttgt aagttttcta gatttcataa aaaatcgctc caaactgatg
    16381 taagaattgt acctagtata gacataaaaa aatcgactgt caatcatttt tatgaaactt
    16441 tttttaattt tctgatagtt aactttgttt ttcagaaaaa taacaattgg ttttattgaa
    16501 gaaaatagtc atgaacaaaa gtatctgaga acttttttac tctcttgttc atgacatata
    16561 gaataaagca gctttgtctt gtaaactccg tttttcaaat aataatctaa agattgctac
    16621 tgaataataa agccgatttc atggtcctct tcgctgctga ttcggtaagg tgactcaaca
    16681 tccgctcccc agctattgat acccccgact cctcttactt ttgcaaaaat cgtaagaacc
    16741 gttcgtcttt ctggcggtag ttcttctata tgtgttgcct gttccagttc ttctgctttg
    16801 aatggtaaag cggaaaaagc aaatggcgta tcctgctgtt caaatcgcaa cgaaaaatct
    16861 tcttgatctt tatccgtatt atccaacgtt tgatttcttg ttagttctac ccatttcgtt
    16921 tccatatgca tgccgcattc ttggggaaca aggtatgggg aaacggggag tcctgccact
    16981 ttataaatac cttcgacacc accagccatc cgatccggat atgtttcccc tgataggcct
    17041 tcatactcaa aaaattttgc tttcgttggc ataatgaacc ttaaacccaa tgcgggtaat
    17101 tccggtaatc ctttttgtcc ataataatgc atattgacaa gcattttacc ctctccagcg
    17161 atttggtagg aaattgtcac ttgggttttt ggtactgtgt tgatttccag tgtaaaaatg
    17221 atttcaaccg tttcagcgta ttcattactt gaatattgat tatttactgg agcaatcggc
    17281 aaatcgatct ttttattgtc caccagtact tctatttctt tgacttttgg atacaatccg
    17341 gctcctaacc acataccgga tttaaatcca aatccgctac ctctgtcatt atcggtcgtt
    17401 gctcgccaaa aaactggaag tgtttcccgg tatagccatt cttttccgtt tttattgagc
    17461 gattccagtc cgcctcttgt ataagaaaaa atataatgcg tattccccga tttgacacct
    17521 aatgtacagt cgccgaagac gattgcaact ttttccgtat ccatatgact tttatttact
    17581 gtatttttca taataatatc ttacctcctg catggcgggt ttttcttgtc catctgcaaa
    17641 aacgatccca ttccctgaga attcatagtc acttggttta tcgtcaaagt caccgccata
    17701 gcgcatcact tctctgcctg tgattggatc agtcacttgg atcgcctgat ctttaaaatc
    17761 ccatatatag cctccttggt acatttcata acgatcaagc aacgtcatgt aagaatccat
    17821 tccacctaac gaattcccca tgtcatgcat atattcacat aagatgaacg gtttttccgc
    17881 tttattttca aggtagtcaa tgatctcttc tggtggcgca tacatgcagc tttttacatc
    17941 tgaaatcgaa tcttcgaatg ctggatttgg aaaattccct tcataatgga caagtcgatc
    18001 ggggtctact tttttgaagt aatcgttcat ttgtcgaatg ttttcttccg cataagattc
    18061 atttccaaga gaccaaaaaa gaatacttgg atggtttttt aatgtttcga aattcgtttt
    18121 tgctcgatca agaacagccg cctgccactg aggtactgca cctggtacgt tgtaagaagg
    18181 ctcaaatgcg cccatttttt gccatgagcc atgggattcg aggttcgttt ccgccatcac
    18241 atggattcct gcttggtcgc ataaataata ccaagtgatc tggttggggt aatgagatgt
    18301 ccgtacgccg ttgatctttg cattttttat gatagccaaa tctttttcca tatcttctat
    18361 agtgatggcg cgtccttttt tcggatgcca ctcatgtcga tttaccccgt tgataatcat
    18421 ccgttttcca ttgaagtaga tgattttatc tttgatttcg attttacgaa aaccgaagga
    18481 atagtcaata tactccgtta ctcttttttc ttcgtctatc gtctccaatt caagcttata
    18541 taaattgggc tgaaaatgcc cccacagtgc cacattttca aatccatcga tgcgaatcgt
    18601 catttgctct tttaacggat attgctgttg atagatttcc ttcccatcag gatctagtat
    18661 cgctacattc agctgactgt ttttggctac ttgctcaaaa gacaaatcta cttcaagttt
    18721 gccattttga catttgtcat ctaagcgagg tttgatccaa agatcttcta aatggttttg
    18781 tcctcgagca actaagaaga catctctgaa gatcccaaaa aatcggaaaa agtcctgatc
    18841 ttccaagaat gcagctgtac ttcttttgta aacctccaca gctagaatat tttcttcttc
    18901 caaataaggt gttaaatcaa attcagacgg agtgaacgaa tcttcggcat acccgataaa
    18961 atgaccattc aaccaaataa acattgcttg ttctacacca gcaaaatgaa tcgagatttg
    19021 ttttcccttt agaccttctt ctaatcgaaa agtcgtcaaa tacgaaccaa ccggattgtc
    19081 tttagcagct gaaaagatcc cttccgcctc tttctcaggg cttgtcgtga aagggggacg
    19141 gcgaaataca tgtccctccc acggatacat cgtattgatg taatggatct tgtcataatt
    19201 tgctaattca atatgactag gaacagtgat ttcatcaaaa gaacaggcat caaaatcttt
    19261 tttataaaat gtttttggtc gtaccgaagg atttggcgaa taacaaaaac gccatgatcc
    19321 atttaaagac tgtttaaggg aggatgtggt ttctacttct tcccttgttg cataacaaac
    19381 atgatcgcta tgtgcatttg attggttcac tctaaaaact tgtggatcat ctaaccattt
    19441 tatatttgct ttcatacttt tttccttctc tctcataact ttttgaaatg aagcagttct
    19501 gtttggaaat caccttgaaa atcggggaag taaagtccaa ctgtcatcaa ttcggccccc
    19561 gaaaatatcc gcttcgaggt ctgttcctga taaagtgcct catcatctaa ccccatcaga
    19621 tatacttcat ggaacgctgg ttgagctgaa gctagtatcc tccctaaaaa cacaatagct
    19681 tcatcttgtt taggagacac aaacatccat gcggcaatat tttcttcaaa aggactcgcc
    19741 aaacgataat aatggccata ctggatcacc ggacgaatcg tttgatagga tataatttgg
    19801 tcttttactt gctttttttc ttcaggagac agctttgtca aatccagttc ataaccaaaa
    19861 acagcgctca tagctgtttc tcctcttgtt ttcaacgaag tagatctgtc ggtctgatga
    19921 tttggtacag ctgatacatg agctgtgatg gacgaaatcg gataagcaag gcttgttcca
    19981 tattggattt tcatacgctc cactgcatcc gtgttatcac ttgtccatga ctgcggcata
    20041 taataaataa acccggcatc aaaccgtcca cctccaccag aacagccttc ccataagatt
    20101 tccggataat cagttgtcaa atgttcaagt agttcataaa gcccgagcat ataacgatgc
    20161 gctacttctc cttgtctctg tgggtcaagg gtaatcgaat agacatctga aagcgaacgg
    20221 ttcatatccc atttgatgta gtcaagagga atcgtatcta aaatctttct catttgtttc
    20281 tcaatcgtct gccgaacatc taaccttgta aaatccagta catgttgtga tctggaagca
    20341 gaaggcattc tacctggctc ctgcataaga aaatcaggat gtgttcgata cagttcagaa
    20401 tcaaccgaga tcatttcagg ttcaaaccat aaaccaaatt tcaatccttt gctatgagca
    20461 taatctgcta tttcacgtaa tccatttgtc aacttgcctt catactcgaa ccagtctcct
    20521 aatgaagaat tgtccgcatc tctttttcca aaccagccat catctaatac gaacatttcg
    20581 atgcctagct cctttgcttc atcaatgatc gccttgattt tttcactatt gaagtcaaag
    20641 taagtggctt cccagttatt gaccagaatc ggccgttcag caaattgatg acgacctctt
    20701 gcgattcttt cacgtagcaa ctcatggtgg atctgactca ttttgttcaa tccctgactt
    20761 gagtaagaaa ggatcatttc aggtgtttga aaagactggc ctgcattcaa ttcccacgaa
    20821 aaacgatggc tgttgatccc accgacgact ctcaattgat tgatctgatc tttttcaagt
    20881 tcgaaagaat ggttgccgga ataaacaaat tgaagtccga tcgcttctcc agtattttct
    20941 gtcgtatgat gatgaactaa agcaataaag ttattcatat ggtgactgct cgttcctcga
    21001 cggctttcaa atacttttgt tccagataaa acagattgcc gtgaaatctg tctttcattg
    21061 acatgtgctc ctggcaaagc aatgacttca tcaaatcgcc tagtatgggc aaagtctagc
    21121 tggaaactag ccgctttttc tagtttgatt gtttctccgg tttcatttct tatctttaca
    21181 gatcgtgtga tcactgctct atcagtaaat atggtataga acaaatcgaa ataaatcgct
    21241 gtttctctat ccttaagtga aatcaccaac gtttgtgcct ctgacttgtc catcacataa
    21301 gtttgaggca agccttcgat ctctggcttc ccatctgtaa tataatggct gtcatatctc
    21361 agatccagta agtctgatcc attcttgcgc tgaatgatcg atgcgggcgt tcgaaagtcc
    21421 atgctgccat gagaagagta ttcttgcggt agtgtatctt tcgacagaga ccggtcagga
    21481 tttaagggaa catttcctga aaatccacga tctcttcggg gataattttt attgtctttg
    21541 tactgcttga ctggtttgcc aaaataaata tgcgccaata cattgctttc ttctatcttt
    21601 atcagataag aaagaaatgt atttcgtaaa tggaagactc ggctcttttc atcaaataag
    21661 atgaaatttt tatctgtttt tctatgcgta atatccatca tttcactccc aacaaaacgg
    21721 aaaccgttta ctttttttta taattcctgt atactgttta ctaaacaatt ttgtcaataa
    21781 aaatttttta tttttttgag gagtgtgtaa aaatggttgg aattagagat gtcgcacgaa
    21841 aggcaaatgt ctctcctgct acagtttccc gtgtgttgaa taacgatcag acgatccatg
    21901 tggctcagac tactcggcaa cggatagtag aggcagctgc tgcgttggat tatgacatca
    21961 gtactcgtaa atatatcaaa aagagaatgc cttcgattgg tgtgatctct acgatcggca
    22021 aagcgagcga agaacaggat atctattaca aacatttgcg agtagggatg gaagaagaag
    22081 ctcgacgtct gcatttaggg atgaatcgaa tctataatct atctgataat cctaaacaat
    22141 gggaagatct agatcagtta ggtgcggtga tcgttgttgg gactgttacg aaagaatcga
    22201 ttaccgcctt gttaaaacaa aacatgcaaa tagtcgtcgt ggataaccct gacatccagc
    22261 aagaggtcga tatggtgtat gcagatcttg aacggatgac taaaaccgta ctggaattgt
    22321 tcttagaaaa tggtcataaa agaatagcct atatcggggg atataaagtc gatatggatg
    22381 aattcggtgg gaagcagatt tctataaatg aaaagaggat gcgtgcctat cagcatttta
    22441 tgatcgatca tcacttagaa aaatatatgt cttatcatct aggtggctgg acagaaaaac
    22501 aaggtgaggt acttgcgact aatctattag aaaatgaatc ccctcttcct actgccattc
    22561 ttgtcgggag cgatccaatg gcaattggtg tctatcgggc attgaaacaa aaaaatatcc
    22621 aaattggaga agagattgtt gtagcaagct ttgataatat cgagacaatc gcaaacctct
    22681 cccctggctt gaccagtgtt caaatcaatg cgaaatcaat tgggcaagca gctgttcgaa
    22741 tggcattgga gcgaattgac ggtatacggg acgaaccaat cattttgact tatcctgcac
    22801 gattgattgt tagggaaagt tttatcccaa gaacaaaaag gtattgaaag agaggttgtg
    22861 aaaagccgct tcgtttcttc atactcaaaa cgaaacggct ttttcacaac ctcttactta
    22921 tacttattca caaaatccaa tgtctgctgg taacaatttt gcgattcttt tagcgtgtaa
    22981 tcaaattggt attcatgggt aatctctttt tttgtatctg tataaaacaa ggattgcacg
    23041 ggtaccttta attctgtcaa ccgattttct aatgcttgac cttgctcctg gaatgagtat
    23101 gcatttccgt cagttatata cgttggcgga aattccttag aaacatggtc agccacacta
    23161 gcttctttca attgctcact cgttttccaa tcttttgttc caatcaacga ccacgcaacg
    23221 gttttgacga aaaacttcat aaatcgatca tccgaatgct gctgtgctgt tagcttcaaa
    23281 tcaacaggtc cgcaataaga aatggttcct ttcagactat cttttggaag tagctgtttg
    23341 attttcattt gtttagcata cgcatcattc gtttgtatag ctgcatattg caatgcgatt
    23401 tgtgaacctg cactatctcc accaaaaaat aattgttcca tgtctagcaa tttgtcgtct
    23461 ttttcttcag ctaataaatc tttgatcagt tcattcactt gaagaacttg attagggtat
    23521 tctgaatcag gcgctaattc ataattcatc gcaaccactg cgatgttggc gtctgaaact
    23581 aattttgtcg caaattcttt gacacccgat ttatctccgc cgacgaatcc tccaccatgt
    23641 acccaaaata gaacaggaac aggccccttg ctgttttttg gatagtagat gtcataagta
    23701 ttctctttat acttggactc atacgtcaaa tctgtttttt tcgccacgtt tttttctgct
    23761 tgatcgaacg tttttttatc agtgatttga acagtatttg aaaacatatg gttgatcaag
    23821 aatgctccag gtctggggga aagctggaag gcaagaaaga ttgcaaacgc gatcccaaga
    23881 atgattccga agaaccatcc gataaattta agaattttct ttttcataag gctccctcag
    23941 tagttagtat taagcattct gattatacca tagtgttatt tcctatcaag ctctcctaga
    24001 tgatttaatg attttcccca aacttcttct gcaatgcttt tgacaaaaga aattttttcc
    24061 attgttcttc aaacaaaaca ttttcttctt ctgttgaagc aaatccacat tgaggactta
    24121 agcacaaacg atccaaaggc agatatcgtg tcgcttcgta gatccggtca atcacttgct
    24181 ggtgctcttc catttcgcca tttttcgtta cagtcaaaat ctaagacaac taatttatct
    24241 tcagagactt tctctaaaag aacaaaactc ccagcacgtt cactatcgta ttctaaataa
    24301 taggcacgga cattttcttg agtaaaataa tgggtctgct acgtcgtcat cccccctgat
    24361 gccgccaaaa tcgaataata atttccgcgt caaacatgta tatttagtgt tgaatcattt
    24421 gagaatatga aaataaaaat ctttttgatc aatcagttaa tcaaattgaa ataacgaaaa
    24481 atctgagaca gaatgacgtt cattctgacc cagatttgat tttgcacaac tcaacagtcc
    24541 ttattggcaa atcgccaaaa taatgtagac taactaaaa
//
LOCUS       DAXWOO010000025        24360 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.025, whole genome shotgun sequence.
ACCESSION   DAXWOO010000025 DAXWOO010000000
VERSION     DAXWOO010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.025"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            23..655
                     /locus_tag="ACGE70_000810"
     CDS             23..655
                     /locus_tag="ACGE70_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692464.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(789..914)
                     /locus_tag="ACGE70_000811"
     CDS             complement(789..914)
                     /locus_tag="ACGE70_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFF8692465.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(1048..1941)
                     /locus_tag="ACGE70_000812"
     CDS             complement(1048..1941)
                     /locus_tag="ACGE70_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFF8692466.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(2123..3049)
                     /locus_tag="ACGE70_000813"
     CDS             complement(2123..3049)
                     /locus_tag="ACGE70_000813"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFF8692467.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(3134..3895)
                     /gene="pflA"
                     /locus_tag="ACGE70_000814"
     CDS             complement(3134..3895)
                     /gene="pflA"
                     /locus_tag="ACGE70_000814"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFF8692468.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(4139..6370)
                     /gene="pflB"
                     /locus_tag="ACGE70_000815"
     CDS             complement(4139..6370)
                     /gene="pflB"
                     /locus_tag="ACGE70_000815"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFF8692469.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(6647..9097)
                     /gene="parC"
                     /locus_tag="ACGE70_000816"
     CDS             complement(6647..9097)
                     /gene="parC"
                     /locus_tag="ACGE70_000816"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFF8692470.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(9109..11154)
                     /gene="parE"
                     /locus_tag="ACGE70_000817"
     CDS             complement(9109..11154)
                     /gene="parE"
                     /locus_tag="ACGE70_000817"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFF8692471.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(11320..11754)
                     /locus_tag="ACGE70_000818"
     CDS             complement(11320..11754)
                     /locus_tag="ACGE70_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFF8692472.1"
                     /translation="MSVKNPTQETIFNYLEETKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            11881..12519
                     /gene="plsY"
                     /locus_tag="ACGE70_000819"
     CDS             11881..12519
                     /gene="plsY"
                     /locus_tag="ACGE70_000819"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFF8692473.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(12651..13526)
                     /locus_tag="ACGE70_000820"
     CDS             complement(12651..13526)
                     /locus_tag="ACGE70_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFF8692474.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(13608..14399)
                     /gene="codY"
                     /locus_tag="ACGE70_000821"
     CDS             complement(13608..14399)
                     /gene="codY"
                     /locus_tag="ACGE70_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFF8692475.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(14417..15817)
                     /gene="hslU"
                     /locus_tag="ACGE70_000822"
     CDS             complement(14417..15817)
                     /gene="hslU"
                     /locus_tag="ACGE70_000822"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFF8692476.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(15831..16379)
                     /gene="hslV"
                     /locus_tag="ACGE70_000823"
     CDS             complement(15831..16379)
                     /gene="hslV"
                     /locus_tag="ACGE70_000823"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFF8692477.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(16416..17321)
                     /gene="xerC"
                     /locus_tag="ACGE70_000824"
     CDS             complement(16416..17321)
                     /gene="xerC"
                     /locus_tag="ACGE70_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFF8692478.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVIYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(17519..18895)
                     /gene="trmFO"
                     /locus_tag="ACGE70_000825"
     CDS             complement(17519..18895)
                     /gene="trmFO"
                     /locus_tag="ACGE70_000825"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFF8692479.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(18864..20942)
                     /gene="topA"
                     /locus_tag="ACGE70_000826"
     CDS             complement(18864..20942)
                     /gene="topA"
                     /locus_tag="ACGE70_000826"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFF8692480.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(21064..21921)
                     /gene="dprA"
                     /locus_tag="ACGE70_000827"
     CDS             complement(21064..21921)
                     /gene="dprA"
                     /locus_tag="ACGE70_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFF8692481.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(21977..22744)
                     /locus_tag="ACGE70_000828"
     CDS             complement(21977..22744)
                     /locus_tag="ACGE70_000828"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFF8692482.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(22737..23597)
                     /gene="ylqF"
                     /locus_tag="ACGE70_000829"
     CDS             complement(22737..23597)
                     /gene="ylqF"
                     /locus_tag="ACGE70_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFF8692483.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            23857..24255
                     /locus_tag="ACGE70_000830"
     CDS             23857..24255
                     /locus_tag="ACGE70_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFF8692484.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
BASE COUNT         6401 a         5198 c         4193 g         8568 t
ORIGIN      
        1 atcaatttaa tttggagata ttatggaaat tcaacgtaga accactaatt ttttacattt
       61 aggctctttt tttgcaactt tgacgatctt attttttcta tgttattcat accttaagac
      121 ctcacatgcg ccttctagtg cattaggctg gttgcttttg gatcattctg atatcacaga
      181 agccgaagaa aatccgtttt ttttgatttt gtcttctctt tgcggcttac tgttttctgt
      241 ttatttcggt gcctattttt ttcatcgaca tcctggtacg ttaaacgaag cttcaagcgt
      301 attgattgca tttaaaacgt tttctttttt agcaattctt ttttattcgt ttaatcacaa
      361 tacgcttttc ttctttatca tgaatggttt agtcgtgatt ttatccattt ttacttattt
      421 tattttttca ctcattttta gccaattgaa atgtccgctc ttctttcgat tagttcctgt
      481 cacctgtttt atttacagct tgttttcctt ttttgtcctt gctttaatgc cactgaccaa
      541 cccttcgacg atccaagtcg caggaaatct gttggatatg ctcttcatcg tcagtctagg
      601 tgtatttatg tggcaaagaa aaaacaaaca aaataaaaag cattttgaag cctgactatt
      661 acgacttcat aaatgctttt ttagttttaa acacttcaat ctaaaagaat aacccaagat
      721 tggataaatt attaatgact cgccattcgt ttcttagtga ttctggatta tcaaatggac
      781 ttcctaatct aatcagctag ttcttcccat aaaatgccat acttgactga atcataatat
      841 tgattattta aaaatctaac ttttcttatc actacttcct ttgtcattcc acatttctca
      901 ccattttttt gcattccttt gtttccagac catgtagtga atctaatagg tggtagatag
      961 gcaaaatact caaataattc ttttaaccat aaaggaagag aaaggctcct cctgcaagag
     1021 tcagtataat ttttgcttac tgctctctta ggttttactc actaaaactg ttccatattc
     1081 tttgatataa gaaaatgcat gcggtataaa tttcttgctc agctcagaaa agatttgtcg
     1141 tttaagaaag gccatttcct ccattgtcaa tgaaggatcc aatgttggta attcaatctc
     1201 aaaattgttt ttttcgacaa taggtaattc taatgtgaat gattcttctt cctttatagt
     1261 attcgtcaca agtagagact gatggaaaat actaggccgg cgatttttat atttattctt
     1321 acgtatcctg tcaacgataa cttctatttg atcaaaaaag tacgattcat ttacgtctcc
     1381 aataagttcg gctaattctt tgaatgcctt cgttacagga agctgattct tctgtacaaa
     1441 aaagtaatta gccaaagtaa gcgggaagtg ttctatagag gaaagtgtga taaaacggta
     1501 cttctcattt cctgcagttg tcctcagcaa ttctgtctct cggctgtttc ttactccata
     1561 aaaatacgct tctttccgtt catcttcgaa acaatacgcc caaataactt ctcccatcgc
     1621 taatttttgt tcatcaacag agagtctctt caattgttca gctatggttt cagcagccga
     1681 agtggcttgt tgcaagtaat ctttaggatc aaatattgga aagttcaaat gatatcttcg
     1741 ttcttccctc ttgattaatc ctgcatcgat caataaatca agaaaatggg aaaatttatt
     1801 ttgcgaaaat tcttttttca attctcttag aatcgtttct tgttctcgat gttcatatag
     1861 ataatcggtc aatgaagaaa aaagcggatc atcggtcagt ttttgataaa agttttcctt
     1921 attttttgaa taatagttca tatatgttct cttttctatc cgttttattt tacttaaaag
     1981 gcttttacgt aaaaaggagg aagaacaaga gtcataattg actgttattc tcctccattt
     2041 cttcggcctt tagtatgttc tatgtttctt tttatatcaa tcgcttatcc attcattttg
     2101 aatgcttcat actaccattt gattagctaa atacagccgt caattgaggt acgacttgtt
     2161 ttttacgtga aacgacacct ttaagcaaag cacggttgtt ggtcaaagtt gtttggaaag
     2221 cttcttctac tttttctttt cctttaccag caacaagtaa ttctgaatca ctgtctaaga
     2281 tgtttgtgac aactaatacg aacagatcgt agccatttgc tttcatttca tcttccattg
     2341 ctgcttctag ctctgcttgt cgatccatca cttcattaag gtccactgta ttgacttgag
     2401 cgatacgaac gttggaactg cccattggga aactttttgc atccaaatca agaagaaccg
     2461 cagctgattt atcacttaga tttgttcctg ctttcaacat ttcaagtcca taagtgttta
     2521 gatcgacttc agcgattgct gcaagttctt ttgcagctgc ttcatcttca ggcgtacatg
     2581 ttggtgattt gaacaacaat gtatctgaaa tgatagctga gagcatgatt cctgcgattt
     2641 ctttaggaat tgttacttgg ttttctttat atagttttaa aataatcgta cttgtacatc
     2701 caactggttc tgcacgatag tacaatggat tagctgtttc aaagtttgca atacgatggt
     2761 ggtctactac agctaaaata tttacttcgc tgatatctga aatactttgt tggaattcat
     2821 tatgatccac aagcatgaca tctgcatttt ctgctttagc tgactctact actcgtggtg
     2881 cagatagctg aaagtggtct aatgcgtatt gtgtttcttc attaggtgta cctagagcaa
     2941 ctgcttctgt ttctttacct aattcatttt gcaagtgtga aaacgcaatg gctgccccga
     3001 ttgcatctgt atctggattt tggtgaccaa aaactaaaat ttttgacatg tcattcgctc
     3061 ctttgtgatt tctcctattt aataatagca aaaaaacgga aagaagcaat acttatcttc
     3121 tttccgatgc gacctatcgc gttaaatatt ttgtatactc ttctgtatga agcaatcgtt
     3181 tagcattttc gacacgttct tttgtaggtg gttcgatccc ttctaatgga tatttcaaac
     3241 caagtgtttc ccacttgtat tttcccatcg tatgataagg aagaatctct actctgtcta
     3301 catttgacaa ggtttgaata aagttgtcta gtcggatcaa atattcatca taatcacttc
     3361 gctcaggaac tagtacatgg cgaatccaaa ccggtttgtt gatttttgac aaatattgag
     3421 ccatttctaa aatattttca ttgtcccaat gcgttagttt tttatgctct tcattatcga
     3481 tgtgtttgat gtcaaataac agtaaatctg tatacttcat caattcttga aaacgtgaaa
     3541 agaaaggttc ttcatacgta aaaggttttc cgcatgtatc caatgtagta tgcatcttcc
     3601 gctcttttgc ttttttaaat agttcgatca aaaaatcgat ctgcaataga ggttctccac
     3661 cactaacagt aataccacct ttatctcccc agtaaggtct gaagcgttct gctttatcta
     3721 aaagttcatc tgctgtgtat tctttccctc cgccaatatt ccaagtatct ggattgtgac
     3781 aaaattggca acgcatgcgg catccttgca taaacacgac aaagcgcagt ccaggtccgt
     3841 ctactgaacc gaaagtttcg atagaatgaa catagccgat tgttttttct tccatttaag
     3901 ttcacatctt ttctttgatt tcattttaag tacaagttag ctgtcgaagc gaagctttct
     3961 gaacaaaatt tttcgcaatt gccgcttcgt tatgtgtaaa atttacaggg acggaacata
     4021 caagtcctgc ccctgtaaat cgtttctttc aaataagcat actgattatt cgattttgat
     4081 gcgatggcta ttcaacgtcc tttcgacgct aagtcgaaaa acttttttca cgaacttttt
     4141 acattctgtc gtgtgaagtt ctcatcaaga catctaattg ttgttctctt gtcaagtcac
     4201 ggaatttaac tgcataacca gatacccgga tcgtcaagtt cggatatttc attggatttt
     4261 ccattgcatc acgcaataaa tcattactga atacattgac attcaaatga taagctcctt
     4321 ttgagaagta tccatctaag acattacgca agttttcgat tcttgtatct tgttcttttc
     4381 caagacctga aggattgatt gtttgggtat ttgaaattcc atccaatgca cagccgtaat
     4441 tcattttagc tgttgagttc aggctagcta ataacccact ttgttccgca ttgtaacttg
     4501 gatttgctcc tggcgcaagt ggttttccag cttcacggcc atcaggagta tttcctgtat
     4561 ttttaccgta aacaacattt gaagtaatcg tcaataatga agttgttggt tttgagttgc
     4621 ggtaagtttt atgacgtttg atttgtccca tgaagtattc caagatccat gaaactaatt
     4681 catctgcacg gtcatcatca tttccgtatt tagggaagtc gccatccact tcaaaatcaa
     4741 ctgctattcc tttttcatta cggattgctt ttacttttgc atatttgatc gctgacaagc
     4801 tgtctgccgc atgagagatc ccagcaatac ctgtagcaaa tgtgcgttgc aagtcagaat
     4861 ccatcaaagc cagctgagct gcttcataag aatatttatc atgcatataa tgaatgatgt
     4921 tcaacgtatt gacatatagt tctgctaacc aatccatcat attcatgaag ttatgtttca
     4981 cttcttcaaa gtctagtatt tctgatgtga ttggacgata ttctggtcca acttgggcgc
     5041 cagtcacttc atcgactccg ccattgatgg catataatag tgttttagcc aagttagcac
     5101 gagcgccaaa gaattgcatg tcttttccga caactgttcc tgatacacag caagcaatcg
     5161 cacaatcatc agaaccccat tgttcacgaa gctgattatc attttcgaat tgaatcgaag
     5221 aactttcgat agcgattctg ctagcatatg ttctgaatcc ttctggaagg tgttcagaat
     5281 acaaaacagt caagtttggt tctggtgaag gtcccatatt tgttaatgta tgaagcagac
     5341 ggaaatcatt ctttgtaact aatgaacgac catctaatcc catacctgca atggataaag
     5401 tagcccagat agggaatcct gagaataatt ggttatattc aggtgtacgc gcgaatttga
     5461 ccatacgtaa tttcattact aagtggtcga tcaattcttg tgcttctttt tctgtgatta
     5521 ctccattatc caaatcacgt tggatataga tatcaaggaa tgctgagata cgtccgattg
     5581 acatcgcagc accattttgt gatttgattg ctgctaaata tccgaagtat agccattgga
     5641 cagcttcttt tgcatttgca gctggtttag aaatatcata gccatatttc gcagccattt
     5701 ctttcatttg cgctaatgca cgatattgtt cagtgatttc ttcacgtaaa cgaatcactt
     5761 cttcactcat tgtatgataa ccagtcatat catgatcttt tttcttttgt tccatcaaat
     5821 agtcgattcc atacaatgcc acacgacgat agtcaccgat gatacgtccg cgaccgtaag
     5881 cgtctggaag ccctgtaatg attttgtttt tacgagcagc acgcatttca gcagtataag
     5941 cgtcaaatac accttgatta tgtgttttgc gccagtcagt gaaaatatgt gtcaattcat
     6001 cgtttggtgt atagccattg ctttttagcg cattgttagc catattgatt ccgccgaacg
     6061 gcatgaatgc ttgtttcaaa ggtttgtctg tctgtagacc gacgattttt tccaaatctt
     6121 tatccaaata gcctgcatcg tgtgaagtaa tagttgatac gatttctgtg tccatatcat
     6181 agacaccgtt acgctcgaat tggatatcat ttaacttttg caattcatcc cataattttt
     6241 ctgttgcttc tgttgggcct tccaaaaagc tgtcatcacc gcgatattca gtgtagttat
     6301 tttgaataaa gtctctagtg tctacgcctt ctttccattt ggtaccttta aatccattcc
     6361 attgctccat tgtatggacc tcctaaattt ctgttttatg taacagtttt tacgtgatta
     6421 gtataacaca gcaccctatc tttgcaaggg ttttcttgtt ttttatttaa caatttttat
     6481 gttttattta catatattta aatcaacgtt gacataccaa catttaagca ttgtgaacac
     6541 tttcatcttt ttttgcaaac gtataaggat ttattaaaaa tgaatgaaaa agagacgcca
     6601 cctgtattaa tacagatggc gtctcttcat taaaatacta caacagttac gactcgatga
     6661 aaatctcagg agcatcgata acttcgatga cttctccacc ttttttctca tccatcgcaa
     6721 atgatccatt cgatgttcga tcacctatag ggaaatcagt tgggtcaatc gttaactgct
     6781 gccctttttg attcatcaag gttatttctt ttgatcttcc ttccgacata tagataatac
     6841 ggtgcggatt ttttttcagc tcacgcatga ccgtcactcc ccgcttcgca cgtgaagttt
     6901 ggctgatttc ttgagcaagc atgcgtttaa cagctcctcg ctgagtaacg attacgatcg
     6961 gcgtatcacc ttctgctaac acaagcagcc cgttgataat cctatctcca tctttcaaat
     7021 tcatagactt cactccagct gcctttgctc caacaacggg aacatctgtt aaaggatagc
     7081 gcaaacccat tccttgatgc gttactaaga aaacatctaa gcctggctga tcttctttca
     7141 gatacacagc aatcagttca tcttcttccg cctttagctt catacagctg agcggacggc
     7201 tcttataggt gcgccatggt tcgaactctg tcattcgtgt ttgcttgatc aatccattct
     7261 tgctgatgaa tacaaatgtt ttctctgctt gaattttttg atatgggaaa acagctaaaa
     7321 tcgattcgtc tacagacaga ttggagattg tttgagaaat gtgctcacca gcatctttcc
     7381 atttcaaatc gggtagttca tggacagggc ggtagatcac atttgcttta tttgtgatca
     7441 gaagaacatg gtccaatgta ctcaattcac ctgagtatag caagtagtcg ccttctcgca
     7501 tcccgatttc ttctggtttg gaagcactat aagaacgaag gcttgttcgc ttcacatagc
     7561 cttctcgcgt cacactgaca attacgtctt cttgtgccac taagacttgc gtatcaattt
     7621 tgatttcttc gatttcgtct tcgattgtcg ttagacgagc ggaagaataa ttttttttca
     7681 cttctcgcaa ttcttttttc ataacagaga aaagctcttt gtcatttgaa aggattttat
     7741 tcaactctgt gatttgcgca atcaagcttt cggattcctt ttgaagctct gtgatatccg
     7801 tgttcgtcaa acggtataat tgcaatgtga caattgcttc tgcttgttct tctgtaaatt
     7861 gaaagacgtc gactaaattc tttttggcgt cttttttgtc tttactttca cgaatcgttg
     7921 cgattacttc atccagaatc gataaagctt tcatcaatcc ttctacgata tgctgtcttt
     7981 tttgagcttt ggctaaatcg aattgactac gttttgttat gacttgcttt cgatgctcga
     8041 tataacttct taaaatgtct ttcagtccaa cttgatgcgg agtcatatgg tcgatagcga
     8101 ccatattgaa attgtaattg atctgtagct cagtgttttt gaacaaataa ttcaaaattc
     8161 cttgagcatt ggcgtctttt ttcaattcga cgacaatttg cagacctgtt ctatcacttt
     8221 catctcgtac ttcagctatt ccatcgattt tcttattcaa acggatctca tccatttttt
     8281 tcaccaaggt cgctttattt acttcatatg ggatttctgt gatcacgatt tgctgtttat
     8341 tacccttcat tgcctctatc tttgttttcg aacgaagaat aacttttcct cgtcctgttt
     8401 cataagcttt tttgatttca tcttttcctt gcaagatccc tcctgttgga aagtcaggtc
     8461 caggaatata ttccatcaat ttttcaaggc tcgcttgtgg atgatcgatc atatagattg
     8521 tgccatcgat cacttcagct aagttatgag tcgggatttc tgttgcatat ccagcagata
     8581 tacctgtcga tccattgacg agaaggtttg ggtatcgagc tggaaggact gttggttctt
     8641 tttctgtatc atcaaaattc cacacgagat cgactgtttc tttctcgata tctgctagca
     8701 tctctccact taacttggat aatctggctt cagtataacg catcgcagca ggtggatcac
     8761 cgtccatact cccgttattc ccgtgcattt cgatcagtac ttcccgcaac ttccagtctt
     8821 ggctcatgcg gaccatcgct tcataaatac tgatgtcacc atgaggatga taattcccca
     8881 tgatatttcc gacagatttt gctgatttac ggaatccttt gtcatacgta ttgccatctt
     8941 tgttcatggc aaataatatc cggcgttgca ctggcttcaa tccatcacgg atatcaggca
     9001 gtgcacgttc ttggataata tatttggaat aacgtccgaa gcggtcaccc atcacttctt
     9061 ctaaagttaa ttcttgtact tcttgtcgat tttccatcag gtgacctcct attctacttc
     9121 aaataactga ttgttttcat ttttatctgc tcgttcttca tctaataaat cattagaaac
     9181 tgaaggagag atttcagtat cctctttttt atctaagata ctgccatctt cttcgagcgt
     9241 aaattgcacg tgattttcga tccattttct tcgaggttct actttgtcac ccatcagtgt
     9301 ggttactcga cgttcagctt gcgcagcatc atcgatacgg acgcggatca acgtacggga
     9361 agtcggatcc atcgttgttt cccacaattg ttccgcattc atctctccaa gtcctttata
     9421 tcgctgcagc atatatcctt tgccgacttt tttaatcatt gctgccaatt cgtcatctgt
     9481 ccacgcatat tcaatgactt gcttctttcc ttgtcctttg gatactttat ataaaggcgg
     9541 taacgcgata tatactttcc cagcttcaat cagcggtttc atataacgat aaaagaaagt
     9601 aagcagtaat acttgaatat gggcgccgtc tgtatccgca tcggtcatga tgattacttt
     9661 gtcgtaatta cagtcttcga tagaaaactc aggaccgact cctgcaccaa tcgtatagat
     9721 catcgtattg atttcttcat ttttcaagat atcttgcatt ttagcttttt cggtattgat
     9781 gacttttcct cgtaaaggta gaattgcttg gaactttctg tctcttcctt gtttagcaga
     9841 tccgccagca gaatcccctt cgactaaata caattcattt ttcttagggt ttcgggattg
     9901 tgcgggtgtc aattttcctg aaagaagaga ttcgcctttt ttacgcttct tcccatttcg
     9961 gctttcttct ctggctttac gtgctgcttc tctcgcttct ctggctttga ttgctttacg
    10021 gaccaacatc tggctcattt cgctattttc ctgcaaataa aagcccattt gttcactgat
    10081 gacgttatct actacggtac gagcaactgg cgtaccaagt ttttcttttg tttgcccttc
    10141 aaattgcaga agattttcag gtacacggat agaaagtaca gcagctaacc cttctctgaa
    10201 atcactacct tccagattct tgtcccgttc tttcagcaaa ccaacttttc tcgcatattc
    10261 attataagat ttcgtcatcg ccgctttcat accagcttca tgggttcccc catcttttgt
    10321 ccgaacgtta ttgacaaaag ataaaacatt ttctgaataa ccgtcattgt attgataagc
    10381 gacctctact tcgatacctt ctttttctcc tgaaaaataa acaactggtg tcaatgtatc
    10441 tttttcttca ttcagataat caacaaattc tttgattcct tcttcgtaat gaaaaatttc
    10501 cttgactggt tcttctccac gaagatcaga aagagagatt ttaacgcctt ttagaagaaa
    10561 ggcagattct cttaaccgtt cggctaaaat ttcatatgaa aatttcgttg tactgaatat
    10621 tgtatcatct ggtaaaaaat ggacagtcgt cccgtttgct ttttttgttt ttcctatctt
    10681 tttcaaagta ccgtcaggtt taccgccgtt tttaaatttc tgctgatact ctacgccatc
    10741 tctaacgatc gttacaatta gccatttcga taaggcattt accacactgg ctcctacacc
    10801 gtgcagtcca ccagaagttt tgtaccctcc ttgaccaaac tttccgccag catgcagcac
    10861 agtaaaaatc acttcaactg ttggtattcc ggaagcatgc attcctacag gcatcccacg
    10921 tccgctatct gcaacagtta tactattgtc ttcatggatg gtcacatcaa tctcactgcc
    10981 ataacctgat aatgcttcgt caactgcatt atcgacgatt tcatagacta aatgatgcaa
    11041 gcctcggctg tcagtggatc caatgtacat ccctggtctt tttctaactg cctctaatcc
    11101 ttccaatact tggatggacg agtcattgta ttcgttgttt actttttttg ccaaggcaaa
    11161 cgctccttca ataatacttt gcggcaaaac cgcaccacga ttatcatact agaaaaggtc
    11221 gtgaaaaaaa agagggtaga cgagaaatca attttaatta tctcctaacc gctttttcga
    11281 cattcccctc tatcgatcat ctcttctttg tggtcatttt tatttttggt tttctttcaa
    11341 cttaccaagt tcaatcttaa tacaacgatc cattacgata tctgtacgtc ctgcttttct
    11401 tagcatctca gctgcttctt tgttttcaat ccctaattgt gcccagaaca ctttagcatc
    11461 agttttcaaa aaatcacctg ctatttcagg taaaaattca ctccgtctaa atacatctac
    11521 gatatcgatt ggatcatcga tttctgtcaa attcgcatag actttctcgc ctagaatttc
    11581 ttcaccagcc aataatggat ttactggtat gattttatat ccattgtctt gcaatacctg
    11641 tgcaaccatg taacttgttc gttctggttt attgctcaag cctacgacgg caatattttt
    11701 tgtttcttcc aaatagttga aaatcgtttc ttgtgttgga ttttttacag acatttctgc
    11761 caccccttgc ttttcttttt attccatcta ttatacttgt atctttcatt ttttcaattg
    11821 ttcctgttac ctattcatgc taaaataaag gatagttttc aacttgaaag agggtaaaaa
    11881 atgaaattga tcttattact tattttagca tatcttttag gttctattcc ttccggtgtc
    11941 tggattggca aattgttttt caaaaaagat atccgacaac acgggagcgg taatactgga
    12001 acgaccaata catttcgtgt attaggcaaa acagccggga ttgttgtcct gcttatggat
    12061 attttaaaag gtacattggc tacttgtttg ccgatgatct tccacctttc tgggattaac
    12121 cctctttggt ttggggtatg cgccattctt ggccatacat ttccgatttt tgctgggttc
    12181 aaaggaggaa aagctgttgc gacaagtgca ggaatgcttt taggatttaa tcctgtattt
    12241 tttgtttatt cttgtatcat ctttatcgtt agtttatttt gtacaagtat ggtgagtttg
    12301 agcagtatga tcagtgcagt attgatcact ttatctacga tcgtccttcc ttatgtagct
    12361 cctgtgattt tggctaagcc caattggtta ctgacgatca ttgccttttt agtttcttcc
    12421 tttatctttt atcggcataa agacaatatc aagcgtatca gaaaccatac tgaaagtcga
    12481 atcccgtttg gtttgaatag cagcaagaaa aaatcctgat actttttcat tccaaagaga
    12541 taaaacggga gtgtgacaaa tcgatgtcac actcccttat tacttacagg atagcttttt
    12601 tacacacctt ggtaaacggt gttccctgct aacttctcgg tcacaacctc ttacttcact
    12661 gtgatggaat attttgtctt gaagatttca tgggcaccta gttgattgat ccctactttt
    12721 tccatcaagt ttccactact gttcaccgtg tcagcgattc cgcaccacgg ttcgatacaa
    12781 acgaaaggcg cttcttttgg ataaggagac cagatcccta cataaggcat atctttatag
    12841 cttaaagtta cgctatggga agatttttca cttcgaatgg cgtacgcatt tagacccctt
    12901 gtttcaaaaa ccaaggcatc ttgctcaaaa agttcacgat tcaaagccag attcgtattt
    12961 gtctgaccta acgtcttttg atccatatcg atgaatgggc ctttcaatgg taggcgtaat
    13021 cttgatttca atggagaaaa tgccagatag aaatcttcaa atgacaatcc ttcttcaaga
    13081 ggtacattga aagcaggatg tccaccgata gaaaagaaca tttctttttc accggtattt
    13141 gccacttgat aatgaaccgt gatgccgtct cctcctaatt catagctgat cactaattca
    13201 aaatcaaatg gatacttttc taatgtctcc ggcgtactcc ttaagatgaa actagccatt
    13261 tcttgtccat gttcaatgac ttcaaaatcc atatcccgag caaatccgtg ttgccccatt
    13321 tgatatgttt tcccttgata aacatactga tcatctttta aacgtccaac aaaagggaat
    13381 aaaaccggtg catgtctgcc ccaatacgct ggatcacctt gccagatata ctctaaacca
    13441 tgttcttttg atttcagact gatcaattcc gcaccgtgtt cagcgatttc ggcgatcagc
    13501 tggtcatttt gaataataac agtcattgcc attcctccaa cttcgactta ctttttaaaa
    13561 tgggaaggct ggaacaaaca tttctatctg ccccagtccc cttacttcta ataactgtgt
    13621 tttgctaact cgtctttaaa acgggaattc aatactttta aataagttcc cttcatgcct
    13681 aacgaacgag attcaatgat tccagctgat tccaattttc gtaaagcatt gacgatcaca
    13741 gaacgagtga tgccgatact atcggcaata ttagaagcag tcagacgacc ttcatctcca
    13801 tccaatgctt caaagatagc ttgtacagcc ttcaattcgc tataagataa tgtgttgatt
    13861 gccatttgga cagccgttgc actccgtaca tctgcttcga tgcttcgaga cttctgataa
    13921 aggatctgca taccgacaac cgtagctcca tattctgcta agactagatc gtcatctgca
    13981 aacgattgtt ccatacgaga taagataatc gtacccaaac gttcgccagc tccaaagatc
    14041 ggaacgacag tcgtcagacc aaatggatat ttctctcttg actctaccgg aaatgcagta
    14101 atatcactag taattgggat attagcttca gttttcatca actgtgccac tgtatcagta
    14161 taagattcag gaaattgttt ctcaacaaac attgttttga ttcgttcatt gttcacatcg
    14221 tgacgttcat tataacctag taatactccg tcacttccta tgatataggt gttgctgtca
    14281 agaataccac ctagtgtgac agccatttta tcgtaaggca aatctgcttg catgtcaaaa
    14341 gtattctttt gctgcaaaag tgtgtttatc ttgcgggttt tcgttagcaa gtctgtcatt
    14401 gtcgtcctcc gattttttat aaaatatagc gacttaaatc ttcatttgcc gcaattgagc
    14461 caagtttgtc atccacatat gcttctgtga tcgttatttc tcccatttgc atatctggtg
    14521 cttcaaataa tagatcttct agtagttttt ccaaaatggt atgcagtctg cgtgcgccga
    14581 tattatctgt gtcacggttg acatcgaaag cgatttgagc gatccgttca attgcttctt
    14641 ttgtaaacgt aactgacaca ttttctgtac cgatcaacgc gacgtattgt tttactaaag
    14701 cattatttgg ttcggtcaga atcttcacaa aatcttcagc tgtcaaatca tctaattcta
    14761 cccgtatcgg gaagcgtcct tgaagttctg gaattagatc acttggttta gacaaatgga
    14821 aagctcctga agcaatgaat aggacgtgat ctgtttggat gatcccgtat tttgtgttta
    14881 cttgtgaacc ttcgacaatt ggcaatatgt ctctttggac gccttctctg gatacttctc
    14941 cagattgctg tgatttagaa gtgatcttat cgatttcatc gataaaaata attccacttt
    15001 cttccgctaa tttgattgcc tcactatgga tatcagcatc cttgactaat tttgctgatt
    15061 cttccttgat caataactct aaagcttctt tgactgttac cgtccgttcc acttttttct
    15121 tcggtgtcaa agcacctaga gtttcattta ggtcgatccc catttgctcc aatccattgt
    15181 tcatcattgg tgcaggtttc ttttggtctt caacttcaat ggttacttca cgactatcca
    15241 gtaaaccctt ttgtagttgt tccaagactg tacggcgatt gattcgaata tcttctgtca
    15301 cttcttcttg cggttcagcg gtattttgct gactagcatt aaaaagattc atcatttgtt
    15361 caaattgatt agtggattgt ttctgttctt ttttgatacc aggtaccaag atttttacca
    15421 aacgtttttc tgcatttttt ttagcacttg tgtatacacg gctgtactgc tctttttcta
    15481 cgatttggat cgcattttct actaaatccc gaaccattga ttctacatct cttccgacat
    15541 aaccgacttc agtaaatttt gttgcttcga ctttgacaaa tggtgcattg acgatttttg
    15601 ccaaacgtcg tgcgatttct gttttaccga cccctgtagg tccgatcatc agcatatttt
    15661 ttggtgtaat atcttgttgc attttttcat ctaactgcaa acggcgatag cggttacgca
    15721 aagcaacggc aacagatttt tttgctgcgt gctgtcctac gatatattca tctaattcac
    15781 ttacgatttg tcttggtgtt tgttttaatt catacatatc tactcacctc ttacagttct
    15841 tctacgataa tattatgatt agtaaagata cagatatctg cggcaatatt taacgcattt
    15901 tctgcaattt ctttcgcact catgtcgttt tgagcatagt ttttcattgc tcgggctgca
    15961 gccaacgcaa aattccctcc tgatccgata gctaagatac catcatctgg tgtgataact
    16021 tctcctgtac ctgaaacaag aagcatttct ttatcattca ttacgatcag catcgcttca
    16081 agtttttgca tagattgctg tgtccgccat tcttgagcta attcgacagc tgcgcgttgt
    16141 agattcccgt tatattcgtt tagtttgcct tcaaattttt cttctaaagt aaacgcatct
    16201 gcaacacttc cggcgaaacc tacaacaact tctccattgt agatacgtcg tactttcttt
    16261 gcagtgcctt tcatgacaac agattcaccc attgttactt ggccatctcc agccatagca
    16321 aatttcccgt ctttttcgac ggcacagata gtggttgaat gaaatggtga ttcgaccatg
    16381 tttgtttcct cctaaatttg ttcttttcct tatttttacg ctcttggatg gaaagaacga
    16441 tagttttttt gtaaactttc ttttgttaca tgggcataga tctgggtagt cgataaattg
    16501 gcatgtccta gcaattcttg gactgtccgc aaatctgccc cattatttaa taaatgagta
    16561 gcaaatgtat ggcgcagcat gtgaggatgg atatcactat ccaaactgct ttttttgatc
    16621 atttgattca agacatattc aattccagta ggcgtgattg gttttccatg atgattcaca
    16681 aaaacgatat catgctcttc gtgatatttt gtcatcaata cctggcggcc ttccctgaaa
    16741 tattcttgca gagcatcttg agcaaaagaa cctagaggag catatctctc tttgttccct
    16801 ttaccatgga tcaacatgac ctctgaatca aaatcaatgt ctgataccga caaactgcta
    16861 cactcactta aacggatgcc tgacccgtaa atgacttcca gtaaagcgcg gttcctcaaa
    16921 tccaaaggac tatctcctgc tacgctgtca aaaagtgctt gcatttcatt ttcataaaaa
    16981 aaacgtggca gtctcaaatt tttctttttg agatgaacgt aagaaaatgg attttctttt
    17041 atcacttctt ctttgagaag atactgataa aacgaacgta ggctggctat cttgcgactg
    17101 atagaatttc tgctatagct aggacgatga tcagctaatg cactcaaata gacacgaacc
    17161 gtaaaatatt ccgccttcag cagatcagcg tctccagatt cctccaaaaa atgtttgaaa
    17221 tggagaatgt cttcttcata tgcttctttc gttttttctg aatatcctct ttcgacaatc
    17281 aaataacgca aaaatttttg ttcccacgtg gcttctgtca ttttctcacc ttccttttgt
    17341 cccagagaat aatttagcac agcgtttata aaaagacaaa taaattgtca gaatttaacc
    17401 aatatttcta agtttgttca caattggcgc aattcctcac attttgtttg agaaattctt
    17461 aagaaaaacg caagaaaaag agcgagacta tccgtctcac cctcttcatg gagtttcctt
    17521 actcatttgg gattgcagtt ttttctaatt ctgtttttat tgcctcatta gcttttaaag
    17581 ctcgttgtgc aattgcttca tatcttgctt ttttgtctcg aatgcgctct ggcagttcag
    17641 gaaacagacc atagttggca ttcatcggtt ggaaatgttt tccttccgca tgagtaatgt
    17701 agtaagccat acttccaata gctgtttctt gtggaaactc gactggttcc atttcttttg
    17761 ctaagcgtgc ggcgttgatt ccagcaagta gtccgctcgc tgcactttca acatagcctt
    17821 ctacaccagt catctgacct gcaaagaaca gatcttcgcg tttcgttgat tggtaagtcg
    17881 gtttcagcaa ttctggcgaa ttcataaagc tgtttcggtg catcacacca tagcgaacga
    17941 attcagcatt ttctaaccca ggaatcatcc ggaaaacacg tttttgctcg ccccatttca
    18001 agtgagtctg aaagcccacg atgttgtata aagaagctgc agcattgtct tgacgcagct
    18061 ggataacagc ataaggacgt ttgccagttt taggatcttc caatccaact ggtttcatag
    18121 gtccgaaaag catcgttttg aatcctcgct gtgccattac ttcaattggc atacatcctt
    18181 caaaatattt ttctttctca aaagatttaa gttctgccac ttcggcattg attagtgcct
    18241 catggaaagc tttgaattcc tcttctgtca tcggacagtt caagtaagct gcttctcctt
    18301 tgtcatagcg ggacttcaaa tatactttat ccatatcgat cgtagctttg tctacgattg
    18361 gtgcagctgc atcatagaaa taaaaaccat ctgaaccatt gaatgctttg atgctttctg
    18421 ctaatggatg agaagtcaaa ggtcctgttg ctactacggt gattccttcc ggtatttcag
    18481 taatttcttc gtttatcact gttactaatg gatgctcttt gacttttcgg gtaattgttt
    18541 cagaaaaaga atcgcggtca acagctaagg caccaccagc tggcactgct gtttcatctg
    18601 ccgaagtgat tactaccgaa ttcaatcggc gcatttcttc tttcaataac cctactgcat
    18661 tagccaaact gtttcctctt aatgaattgg aacatactag ttcagcaaaa ttacttgtgt
    18721 gatgcgcctc agtcgatttg actggacgca tttcatataa acggacaggg actcctgctt
    18781 gtgcgatctg ccaagcagct tcacttccgg caagccctgc tccaacaaca tttaccattt
    18841 tcaatgaaag aacctctttt ctattatttt tggatgtctt cttcgtaatc tccatttaca
    18901 cagactactt gtttaccgcc tttgactttc ttctcgacca gatatccgtc acattttgga
    18961 caatttctgc ccactggttt atcccaagaa gtgaattcac attctgggaa acgggaacaa
    19021 ccatagaata gtctgttttt cttagatttt cgttcaatga cttgtccttc atgacatact
    19081 gggcaagtca caccgatttc ttttacgatt ggtttcgtat tccgacaatc aggaaaatta
    19141 ctgcaagcat aaaacttccc atatttccct aattttatca ccatcggatg tccacagaca
    19201 tcacaatcga atcctgctgg ttcatctttg atttgaattt tttcgatttt ttcttccgca
    19261 ttcgtcagtt ctttttcaaa cggtttatag aaacgatcaa ccactttgac ccacgcttcc
    19321 gtgccttctt caattttatc caaatctcct tccatttctg ctgtgaaatg gatatcgacg
    19381 atttgaggga agaattcaca gattaatgaa ttgacaattt ctcctagttc agtcggctca
    19441 aatctttttt gtgtcaattt tacatagtag cgtctttgga tcgtatcaag tgttggtgca
    19501 taagtggaag gtcgccctac cccgttttct tctagtgcac gaatcaacgt agcctcacta
    19561 aaacgtgctg gtggttgagt aaaatgttgt tttggttcaa tatcaacaga ttggactaca
    19621 tcgccttctt caagttctgg aagaatattt tctttctctt ctttgccgtc atctctacct
    19681 tccacataca cttgcatgaa tcctttgaat ttgatttttg aaccgttcgc aatgaagatg
    19741 acaccatttt gttctaatgt cactttcatt gtatctaaaa tagctggtgt catctgacta
    19801 gccacaaaac gtgaccagat caatgtataa agtttcaatt gatctttatc taaatattgt
    19861 ttgagttcat ctggtgtacg aagcacactc gttggacgca cagcttcatg ggcatcttgt
    19921 gcgccttgag catttttgat ctttcggtga tcatgtctag aaaattcttt tccatacgta
    19981 tcttcaataa atgctgcggc ctctgctttt gctgtatcgg caatccgggt agaatcagta
    20041 cgcatgtatg taatcaagcc gactgttcct tgtttaccca aagtgattcc ttcatataat
    20101 tgctgtgcta ccatcatggt ttttcttgtt cgaaagttta acttacgggc agcttcttgt
    20161 tgcaggctac ttgtagtgaa aggattcgca gggtttcttt tgcgctcttt cttttctact
    20221 tttttaactt cgaagtctgt tccatcaatt ctagcagtga cttcttttac actttgtgca
    20281 tctggtaatt tctttttctt accgtcgatt ccccagaaat ttgctttgaa tttcttgcga
    20341 gcttttttaa aattaccgtc gatgctccaa tattcttctg gaacaaattc tcggatctct
    20401 ttttcacggt caataataat ttttaaagcg acagattgga cacgtcctgc acttaaccct
    20461 ttcttgacct tacgccagag aataggactg atcgaataac caacgattct gtctaaggta
    20521 cgacgagctt gctgtgcatc tactaaatct acatcgatcg ttcttggttc cttaaaagct
    20581 gctttgactg cgtcttttgt gatttcattg aagaccaccc gatttttttc tttcagatcc
    20641 aacccaagta ggtacgctaa atgccaagca atcgcttctc cttctctatc cgggtcactt
    20701 gccaagtaaa ctttttgagc tttttttgct gcggctttca ggcttttgat gacatcgcct
    20761 tttccgcgta tagaaatata atggggttca taattgtttt caaaatcgat acccatttta
    20821 cttttcggta aatctcttat atggccaaca ctggccatta ctttatagtt tcgtccaaga
    20881 tatttctcaa ttgtcttggc ttttgcaggt gattctacaa tcactaaata tttatacgcc
    20941 attactgaat ggactccttt ccattgtcgt gttcctacta ttaaaagagg attcatacaa
    21001 atcgtagcta atcatatcca ttcagaaaac gtttgtcaag aaaagcaaat gaaaagtatg
    21061 gtgttattct ttaaattctg ccaagatatc ttcacccgaa accgtgcaaa ctgcaccgtc
    21121 ttggatcaaa tgatgacatc cggtagaatg ttctgttaaa atatttcctg gaacagaaaa
    21181 aactgttctt ccaagatcca atgcttgctg tgctgttatc agagagccgc tttttttctt
    21241 ggcttcaaag acacatgttc ctttagacaa agctgcgata atccgatttc tcattggaaa
    21301 acaatgcttt ttcgggccgg attgaggagg atattcactc aaaagaagat gatcatttgc
    21361 aatcacctct tgcaagtcac gattttcagc aggatagact tgctctatcc ctgttccaat
    21421 gactgcgatt gtccgtccac catgagagat tgctgcttgg tgaccgtatg tatctatccc
    21481 tttcgcgcag ccactgacga tcacatatcc agcttctatc actggtttta gtaaataata
    21541 aactgttttt ttggctaaag cagaagtgtg tcttccacca acaacgctta acatgttaca
    21601 agaaagtaaa gatagatcac cattataaaa cagtacaatt ggcggatagg tcaagtgaag
    21661 cagctgaggg ggatatttgg gatcaaagca agtgataaac gactgtttcg attggatttt
    21721 gccccagtta gcgtgaattt cctgccaact tttgcgaaac agctcccggt gtttggttat
    21781 tttacctatc ttaatgatgt cttcaaatcc gatttcttta taaggatact gcatcatcca
    21841 gttagcaagt gcccattttc cttgactact gattcctttt gtataagcaa gtttcaataa
    21901 attttcttct atttgataca tcataaaacc gcctttcgtc tctctttcta ataagatacg
    21961 taaaagcggc tgttttttat tgtaaatatt tttttacagg tgcaaaactt ttacgatgga
    22021 ttggcgtaat tccttgtcgt tcaagtcctt ctaagtgttc ctttgttcca taacctgcat
    22081 tatgcgagaa accatatcca ggataaagca cgtcatattc tttcatcaat tggtctcgat
    22141 atactttagc aatgatactt gcagcagcaa tcgacacaga tcgtgcatcc cctttgatta
    22201 ttttttcctg ctgaataggc aaatccaagg tcatcgcatc gatcaacaaa tagcttggta
    22261 caatggacaa tttttctact gcttggatca tcgctaattt acttgcttca taaatattca
    22321 catcatcgat tgtctgctga tcaacgatcc cgatcccgat tgccagggct ttttcttgaa
    22381 cttgttgata aagaagttct cgctttttct ctgataattg tttggagtca ttcagcccta
    22441 atatgatctc atttttcggc aagataactg cagctgccac gacaggtcca gctaatggtc
    22501 ctcttcctac ttcatcaatg ccggcaatcc attggtgacc ttgtgcgtac ccttgctttt
    22561 caaagcaaaa catttcttca tatttttcct taattaatgc ttcctttttc aatcgtcgtt
    22621 caaaagaagc cagtgcttgc tgcacgcctt tgcgtggatc atttcttaat gattctagtg
    22681 taggatcatc tattctctgt atgcttttca atcgttcttt gatactttgg atggattcag
    22741 tcatcacttt tttgctccat ttcttcgcaa cgatccagtg tgtatcgtcc aagttttcct
    22801 tgacgaatat catgcacgac taattcgctg ccacgatcat agtcttcttt atacccacgt
    22861 tttgctgtaa tatccatcag taattccggt gccaattttt cttcttgctc gattgataaa
    22921 ccataacgat cgctgattct tcctgaataa tatcgggcaa aaaagtctaa tccgtaaatc
    22981 gctaaatcat ctaaatggac aagctggtct ttgatcgcgc ctgtcagcgc tagttttttg
    23041 ccgatttctt catcttcaaa tttgggccat aaaatccctg gtgtgtctaa caattccaac
    23101 tcattaccta aacgaagcca ctgctgacct tttgttacgc caggcttatt tcctgtctgt
    23161 gcaattttct tgcctactag tcgattcatc aacgtagatt tcccaacatt tggaatacct
    23221 aagaccattg cccgaatagc gcgaggcttg atcccccgag cagcatctct tgcccgtttt
    23281 tctttcaatg cttctttagc cgcaggtact agacctttaa ttcctttact ttcttgtgcc
    23341 gtgatagcta atgcaagatg gccttgttta cgaaaatatt cttgccacag ctttgtctgt
    23401 tgtggatctg ccagatccgc tttattcaat aaaacaaggc gaggtttttg ctgaatgatc
    23461 tgatctagca ttggattacg agaagataaa ggtaatcgtg cgtctaccaa ttcaaatacg
    23521 atgtccacat atttcatttt ttcagaaact tctcgtcggg cttttgccat atgtcccgga
    23581 aaccattgga tggtcattgt tctctccatt cctttcatac cggaatattc cccggcttta
    23641 cttgtttttt tctttttcaa tataaattca cttttttatt ataaagaaat tccatctatt
    23701 agttaagtga aagtgaacca gtcctcattc tatcacatct tcactttttt gatgatgcaa
    23761 atttttattt tggtttctat atatgaatct aaatttttga cagcgtttcc taaataagag
    23821 atgatagacg aacaaaacag aaaggagacg aaacaaatga atagtatggt ttttgttaat
    23881 tttcctgttg ctgatgtcaa tcgttccgtt gctttttatg aaaagctggg ttttaaaaaa
    23941 aatgaagaat tttctactga agaagctggt tcaatggtat gggatgacac tttttggata
    24001 atgcttctct ctcatgattt ttataaaaaa tttataaaag ataaagaaat agctgataat
    24061 aaaaaaacaa gtggtgcttt gatttcgttt agtgttgaaa atgcggatga agtaaaaaaa
    24121 tttgctcaaa ttgcaaaaga aaatggtggt actttccacg aagtagatat gggaatgcct
    24181 gaagaagaaa tgttttcatt agaagtccaa gatctagacg gaaatacatt agaacccgta
    24241 tggatgaaga tgtaagcaga taactaatct gacgtttttt ataaaaaaaa aacaaaggcg
    24301 gatgatacga taagtattct ccgcctctat tttttttatt cgtacaatct cttttttaca
//
LOCUS       DAXWOO010000026        24151 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.026, whole genome shotgun sequence.
ACCESSION   DAXWOO010000026 DAXWOO010000000
VERSION     DAXWOO010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24151
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.026"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(567..1592)
                     /locus_tag="ACGE70_000831"
     CDS             complement(567..1592)
                     /locus_tag="ACGE70_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8692485.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            1867..3552
                     /locus_tag="ACGE70_000832"
     CDS             1867..3552
                     /locus_tag="ACGE70_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFF8692486.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTNKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            3566..4849
                     /locus_tag="ACGE70_000833"
     CDS             3566..4849
                     /locus_tag="ACGE70_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFF8692487.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            4919..5788
                     /locus_tag="ACGE70_000834"
     CDS             4919..5788
                     /locus_tag="ACGE70_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8692488.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            5785..6621
                     /locus_tag="ACGE70_000835"
     CDS             5785..6621
                     /locus_tag="ACGE70_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8692489.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            6647..7714
                     /locus_tag="ACGE70_000836"
     CDS             6647..7714
                     /locus_tag="ACGE70_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFF8692490.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            7711..8538
                     /locus_tag="ACGE70_000837"
     CDS             7711..8538
                     /locus_tag="ACGE70_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFF8692491.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            8583..9608
                     /locus_tag="ACGE70_000838"
     CDS             8583..9608
                     /locus_tag="ACGE70_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFF8692492.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            9605..11788
                     /locus_tag="ACGE70_000839"
     CDS             9605..11788
                     /locus_tag="ACGE70_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFF8692493.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            11788..12916
                     /locus_tag="ACGE70_000840"
                     /pseudo
     CDS             11788..12916
                     /locus_tag="ACGE70_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
     gene            12913..13590
                     /gene="pgmB"
                     /locus_tag="ACGE70_000841"
     CDS             12913..13590
                     /gene="pgmB"
                     /locus_tag="ACGE70_000841"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFF8692494.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            13674..14201
                     /gene="msrA"
                     /locus_tag="ACGE70_000842"
     CDS             13674..14201
                     /gene="msrA"
                     /locus_tag="ACGE70_000842"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFF8692495.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(14402..15088)
                     /locus_tag="ACGE70_000843"
     CDS             complement(14402..15088)
                     /locus_tag="ACGE70_000843"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFF8692496.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            15168..16706
                     /locus_tag="ACGE70_000844"
     CDS             15168..16706
                     /locus_tag="ACGE70_000844"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFF8692497.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            16826..17293
                     /locus_tag="ACGE70_000845"
     CDS             16826..17293
                     /locus_tag="ACGE70_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFF8692498.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            17827..18255
                     /gene="rplM"
                     /locus_tag="ACGE70_000846"
     CDS             17827..18255
                     /gene="rplM"
                     /locus_tag="ACGE70_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFF8692499.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            18271..18663
                     /gene="rpsI"
                     /locus_tag="ACGE70_000847"
     CDS             18271..18663
                     /gene="rpsI"
                     /locus_tag="ACGE70_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFF8692500.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(18751..19977)
                     /locus_tag="ACGE70_000848"
     CDS             complement(18751..19977)
                     /locus_tag="ACGE70_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFF8692501.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTSDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(20049..20351)
                     /locus_tag="ACGE70_000849"
     CDS             complement(20049..20351)
                     /locus_tag="ACGE70_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003013637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8692502.1"
                     /translation="MTIAANNDTSVRLAVNWGEVKFLCAYPKQFKNFITTILGDDFIE
                     SRVYLNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKDFMNTFKIT"
     gene            20899..21618
                     /locus_tag="ACGE70_000850"
     CDS             20899..21618
                     /locus_tag="ACGE70_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFF8692503.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            21620..22462
                     /locus_tag="ACGE70_000851"
     CDS             21620..22462
                     /locus_tag="ACGE70_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFF8692504.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            22459..23409
                     /locus_tag="ACGE70_000852"
     CDS             22459..23409
                     /locus_tag="ACGE70_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFF8692505.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            23424..23657
                     /locus_tag="ACGE70_000853"
     CDS             23424..23657
                     /locus_tag="ACGE70_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFF8692506.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            23988..>24151
                     /locus_tag="ACGE70_000854"
     CDS             23988..>24151
                     /locus_tag="ACGE70_000854"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFF8692507.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINAL"
BASE COUNT         7924 a         4139 c         4954 g         7134 t
ORIGIN      
        1 tattgtcaca acctcttttt tggtttgtct aaaataattt gtctaactag aagaaaagtg
       61 ggaaaggatc ttaacaacat cggttcgtat ccaattaagg tatcggaacg atcatcttcc
      121 aagagaagtc aatcaacaaa agtaaagata gatcattttt tgaatgcaaa ataaattgat
      181 gggaagatag agcggttatt tttaatattt tgctagctat tattgaataa ttcctcgaat
      241 aaaacaaaac cagtagacat gtctgttatt ctatttatgt tttagagata agtatcccca
      301 aataacatgg attgaaataa gggtaaatgt gtttaaaaaa acgaactata aaaacttata
      361 cacaacgtgg ttaaaaatag ttttttcttc cctgtggaaa aaatagttta acacaaacat
      421 tatagttatc cacagctatc taaaacagat ggaaaataag aaaaattcgc taaaataccg
      481 ggagggggta acgagggatg agtgattgaa aaaagaacag agtaactgac ttatcctcag
      541 tgtttctgtt cttttataat tctgttctac atttcttgtt gttggatcga atctgtgaga
      601 gtaatctcgg ttgaaagaat gattcttttt tcttcagggt attggtggtg gattagttgg
      661 aataaaagtt ctgtggcttt gattcctaac tgatttgtcg gaatgtctat ccgagtcaac
      721 tgtggcgaaa atagggagga caataaggga tcactgaatc ctgcgatcga aagatcatca
      781 ggtatctgct ttccagattg ttctgccact ttgtaaatac tcaatgattt taatgcatcg
      841 gtagaaaaaa tcgcagttgg tctttctcgt agcttcatta aatcattcat tgctgccatg
      901 ctatcttcta agcgaaagcc gccgtctata atccattcat tacggatatc taggtggtga
      961 tcaaataagc attgacggta accattgaga cgatcattgg atacataaac ttctgtcgga
     1021 gcatggagac agccgatatg acggtgtcca ttcttgatca gtgtttccac caaatcgtaa
     1081 ctatcctgga aattatccgt gtccactgag tagatgttcg tgtagtcgtt tccgactttt
     1141 ccgattacca caataggaat attgtattga tccaattcaa aaagaagtgt ttcatccatc
     1201 gtagagctta aaataataat tcctcggatc attttttctt ggatcttttt cgcgcatttt
     1261 tctagctctt cttctggtgt ttttgccgtt tgtagaatca agtcgaagtt attttgttca
     1321 gaaacggcag aaatggattg aataacatct gaaaaaaaag gattgccata ggtcgtatga
     1381 gaagtacgat gagataatgc tagaatcgcg tcaaaaccat ttcctgacag tcctcttgca
     1441 attttgctcg gttcataatt caattctttg attgcttgaa gtacacttgc ttttgcttta
     1501 tcagagatat ttggctggtt atttaatact ctggagacag ttgattttga gacattagct
     1561 tttttcgcaa tgtcataaat agtaggtttc aattttatat gcacctcctt tttgggagcg
     1621 ttttagaata attccatagt acaatagaca ttcttttttg tctatgattt tgaaaattta
     1681 ggaatagctt gcatgatcat gagagaccat atttttttaa gaagctatta attattgata
     1741 cataagcttt cttatttgta tttttgtgta tatcaaaatt atttaaaaaa atgctattga
     1801 cagtaagcgt tttccataat atgatcaaat cgttgggaac gctcccaaaa aagaaagggg
     1861 aatccgatgt atacaaaact tttacaagcg atttatcgcg actcagattt gtctgagaaa
     1921 accgcctcta tgattgaaca aaaagtcagt gtttatcagc agaagaaagg ccaggcaggc
     1981 aaactgcatt gggacgaaga agatattttc ttgattactt atggggatca attttttgaa
     2041 gagcagcaac caactttgcg tacatttaag aaattttatc aaaagtattt ggcagataca
     2101 tttagagttg tacatttctt gccatttttc ccatatacat ctgatgatgg tttctccgtt
     2161 gtaaattatc aggcaatcaa cgaccgtttg ggcacatggg aagatataga agaaatgaga
     2221 agcgaaacac ggttaatgtt cgactttgtc tgcaatcata tgtctgccga aagtacttgg
     2281 tttcaggaat atttaaactt gaatgatacg tacaaagatt tttttattga tttgccactg
     2341 gatacagata caagcagcgt gactagacca aggaccagcc cgttacttac aacgttcatt
     2401 gacaaaaacg ggaaagaaaa acacgtatgg acgactttta gcgaagatca agtagacttg
     2461 aattttaaaa atccagaagt tttattgaaa atgatcgata ttcttttgtt ttacttagaa
     2521 aaaggcgctt catttattcg cttggacgct gtgggatttt tatggaaaga actaggaact
     2581 tcttgtatac acctgccaga aacgcataaa atcatccaat tattccgtgc aatcgctgat
     2641 gaagcagctc caggaacggt attgatcact gagacaaatg tgccacacaa agataatatc
     2701 agttattttg ggaatggcga gaatgaagca cagatggtct atcagttttc gctaccacct
     2761 ttgacccttc aagcgattcg ttcgggtaat acaaataagt tgaaagaatg gttaaaaacc
     2821 atcgatttcg catcgaaaaa aacaactttt tttaattttt tagcctccca tgacggtatc
     2881 ggattgaatc cggtacgagg tattctgccg gaaaaaacga tccttgaatt agtggaacag
     2941 ctgaagaaag agggtgcccg agtaaactac aagaaaaacc cagatggaac gaccagtcca
     3001 tatgagataa acgctacgta tctagatgcg ttaagttctc caacagatac gacggaatgg
     3061 agaagcaaac gctttttagc tgctcattca tttttggtaa cgttaccagg cgtgccggca
     3121 atttatattc aaagtgtgct gggaagtcga aatgattaca aaggcgtaga aaaaacagga
     3181 gagaatcgaa aaatcaatcg gcaaaaattt ttgttagcag atattgagaa agagttgcag
     3241 acagatccat taagaacagc agtatatggt gaattgaccc agatgctatc tattcgaaaa
     3301 aaagaaaaag ccttccatcc gaatagtttg atttacttac tggactcagc agattctttt
     3361 atcagtttta ttcgcgaagc gaaggaagag aaagtcttag ttatccataa tttttccaat
     3421 caatcagcag gttatctttt accaaaagga gattacctag acctgttttc tggacaaatt
     3481 atccattgca gtcaagaacg aaagttgata ttccagccgt atgaatatat gtggctgaaa
     3541 ataataaaat aagaggtgga aaaaaatgaa aaaaattatt gttactacct gtctgctagc
     3601 tactggattt cttcttactg cttgtggtga aagtcaaagc tctaacgaat cacaacagac
     3661 aacgattcaa ttcatgcact cttctgtcga acaggaacga ttaagtgtga tcaatgaact
     3721 ggttgctgat tttgaaaaag aaaatccaga catcaaaatc aagcaagtgc cggtagaaga
     3781 atcagcgttc aatacaaaaa tcgttacgtt agctcgttcc ggtaaattac cagaagtaat
     3841 ggaagtcagt caggattttg ctaaagtgat ggataaagat gaactgattg atcagacggc
     3901 agtccaaaac gttatcgagg aagctggaga agataattat tatgacggag ccaaaaacct
     3961 tgttcgttca gaagatggaa aaagctatat tgctgcacca attagcggct gggttcaagg
     4021 tatttggtat gacaaagaaa aattatcaga agctggtttt tcagaaccag aaaattggga
     4081 tgatatttta aaaattgctc aacatttcac agataaagag aataaacaat atggaattgc
     4141 tatgcctact gctgaaggaa caatgagtga gcaagcgttc tctcaatttg ctttgtcaaa
     4201 tggagcaaat gttttggatg atgaaggcga ggtaacgatc gatacggaaa agatgagaga
     4261 agcattgtcg ttttatcaaa acttatccca atacacgatg ccaggctcta atgatgtgac
     4321 agaaatcaaa gatgctttca tgaatggaac agcaccaatg gcaatctatt caacttacat
     4381 tttgccttct gtttatgaag cggggaactc agaaaatatc ggttttgcta tcccaacaca
     4441 aaaagatcaa gctgtctatg gcacagtatc tggattgacg atttcttctg gtctagacga
     4501 gaaacaaaaa gaggctgctg aagcattcac agctttctta tctcaaccga aaaacatgga
     4561 aaagtgggtc ttgatgtctc ctggtggtgc tcaaccagtg aataaacaag tagtcgaact
     4621 ggatggttac aaagaaaatg aagtcatcaa gtcttttggc gagctgccat ctgagattgc
     4681 tagtgctttt gatgaagtac aagtctttgg actagtcggg gaaaagaatt tcacgaaaat
     4741 gggagatatc acaagctcag gtgtgatcgg aaaagcagtg aaccaagtaa ctgtcggaaa
     4801 cgaagatgtc gatcaagctt tagctgacgc acaaaaaaat acgaaataac aagaagttct
     4861 gccaatggtg ttcgaaaaaa gtagatagct attggcagat tatcaggggg aggtttcaat
     4921 gaatcgtcca aggaaacatt ctgacttaaa attagccatg ggacttttag caccaagcat
     4981 gcttttatta gctgttttag tgctgattcc aatgctttcc aatattcaga tcagtttttt
     5041 cgatatgccg ctgaatccta actcagacgc cgtttttatc ggattaagca attatcttga
     5101 aattttaagt gatagtgaat tttatcgttc tttaggttta acagttgctt acacattctt
     5161 agttgtagca ggcagtacag ctttaggatt agccgtagct attttgctta accgcaagtt
     5221 cagatttagg aaaacggtgc gttcactgat tttattaagt tatgttacgc catctatttc
     5281 tttgattttt gcttggaaat atatgttcaa taatagttat ggaatcgtca atgatctgtt
     5341 agtagatagg ttgcagcttt tcaacgaagc accgctgtgg ttcgaccagc cagttgctag
     5401 cttctttatg gtcgttttgt ttgctatctg gagatactat ccgtatgcgt ttatttcatt
     5461 tttggctatt ttgcaaacag tcgacaaaac gctttatgaa gcagctgaaa tggacggtgc
     5521 aaatgcttgg cagaagttca agatcgtgac actgccggca atcaaaccag tactagcaac
     5581 tgtgattgtt ttacgatcta tctggatgtt ctatatgttt acagatgtct atctactgac
     5641 gaataaagtc aatatcttag gcgtttattt gtatcagaca gcctttgcgt tcaatgatct
     5701 tggaaaagca gcagctatat cgattctatt gttcatcatt attgcagcgg tcgtcttgct
     5761 ctcacgaaag aaggtggact tgaaatgaaa aataaaaaga gcaggaaaat cttattttac
     5821 gcgacgattt tgttgctatt agctgtcaat ttgtttccat ttttgatcat gctgatgaca
     5881 tcattcaagg attcacaaga agcaattgca acgacacaaa ctatttttcc gaaagaatgg
     5941 acttttgaac attatcagga tattttcaat gctgagattt ttccattcat catgtatttc
     6001 aaaaatagtt tagtgatctc cttgatagcc tccgtaactg cagtagtgat tgggatcagc
     6061 ggggcatacg ctttgtcaaa actacgcttc aaagctcgct cgaaaatcaa tgctagcttt
     6121 tacaccgttt atatgttttc cggtattttg atcgttgttc cattatttaa aatcatttcc
     6181 ggtctaggtt tgtatgatac caatacagca ctgattatca cgatggtcgt tcaaacactt
     6241 cctactgcga tattcatgct aaaaagttat tttgacacga ttccgactga tattgaagaa
     6301 gcagcaatga tcgatggtct tagccggatc cagatcatta ttcggatcat tataccattg
     6361 gctatttctg gaatcatttc tgtcttcgtt tattgcttta tggtttcgtg gaatgattat
     6421 ttatttgctt cgattttcct gtcgacctcc gaaaaattta ctttaccaat cggtttgaac
     6481 acattattca gtacaccaga ctacatttgg ggacgaatga tggccgcttc attggtgacc
     6541 gctttaccag tcatcattat gtatgcgtta tctgaacgat ttattaaagg tgatttagtc
     6601 gaaggcggcg taaaaggata aaaagaaaag aaagaggaaa gaaaagatga aaaaattagt
     6661 tgcattagaa ccaagaatag ccggattcaa agattacgaa gaccgtgaga tcgaaaagaa
     6721 cgaagtacgt atccgagtaa ttttcgctac tccaaaacca ggaacagaaa ttgtcgactt
     6781 tagaggatta agtccattta tcgatgaaga attttcagaa gaatggcgcc tattcttgcc
     6841 aagagaagaa ggaagccaac gcggtgtggt atttggcgaa tttcaattag gaaatatgat
     6901 tgtcggtacg atcgaagaaa cgggcagttt ggtgacagag tatcaaaaag gagagttagt
     6961 ctgcacttat ggacccattc gtgaaacgct gattacaaac ggtgttgata attataaact
     7021 acgtaaactt gatcggttgg atcaatggaa aaatgcagta tgttacgatc ctgctcaatt
     7081 cgctttaggt ggtatccggg aagcacatgt tcgagcaggt gatgctgtag caatcttcgg
     7141 tttaggcgct atcgggcaaa ttgcgattca attagctaaa aaagcaggtg ctactaccat
     7201 cgttgggatc gatcccatag cgcagcgtag agaattagca gaaaaacacg gtgccgatta
     7261 cacgctagat cctatgaaag cagatgtcgg ttttcgtcta aaggaattga cagataaacg
     7321 tggagtagat tcagttattg aaacgagtgg cagtgctgcc gcattacaag cttctttaat
     7381 agggatcggc tatggcggga tcatttctta tgttgccttt gctaaacctt ttccagctgg
     7441 gtttaatctt ggtcgcgaag cacatttcaa taatgcaaaa atcgtctttg ctcgagcatc
     7501 tagtgaacca aacccagatt acccaagatg gaaccgtaaa cgaatcgagg acacatgttg
     7561 ggaattattg atgaacggtt acttggattg tgaagaaatc attgatccag ttgtgccatt
     7621 tgcagaaagc gcagaagctt acatgcgtta tgtagaccag cacccagaag aaagtatcaa
     7681 aatgggcgtt caagtccgag gaggagaaca atgaaattcg gtacgcaaga tcaagcattt
     7741 ttcccaagag acattttaga aaaatttaga tttatcaaag actgtggatt tgaagaatac
     7801 gaaatcgacg gtaagttgtt aatagaaaat atcgacacag tcaaacaggc aatcaaagaa
     7861 acagggatcc cagtagtgac agcttgtaac ggctacgatg ggtggatcgg tgactttatc
     7921 gaagaacggc gtcttaatgg tgtacgtcaa attgctgaga ttttgcgtgc actaagcgag
     7981 gttggaggca aaggaatcgt tatcccagca gcttggggaa tgttcactta tcgattaccg
     8041 ccgatggttt caccccgaag caaagaagga gactataaag cagtcagcga atcacttcgt
     8101 tatttagata aagtagccga agaaacaggt acggttattt atttggagcc tttgaaccga
     8161 taccaagacc atatgatcaa tttgttagcg gatgcgcgtc attacattga ctctaatcaa
     8221 ttgaaacatg tgcagatcat cgctgatttt tatcatatga atattgaaga agatgatatt
     8281 tcaaaagcat tgactacgca aaaagagtat gtgggtcata tccatttagc agataatcat
     8341 cgctaccaac caggatctgg cgcaattgat tttactcgcc attttcaaac gctacttcaa
     8401 aatgagtatg aaggttatat ggtatatgaa tgtcgtttgc gaggagaaaa tgaagtgaaa
     8461 atttatcagg aatcattgca atacttgaaa aactgtctgg aaacagcgcg tcatttaaat
     8521 gatttagaga aagaatagag aaagaataac aataagtaaa taagcaaagg agggagaaat
     8581 aaatgaagaa agtagcaatc ataggcgctg gacaagtagc agaaaaagta catgctgctt
     8641 attatcaaaa aagatcagat gtcttagaat tggccgctgt ggttgatcca gtcagccagc
     8701 gagcagaaga cttttgccga cgtacaggat tttctcatgc gtatacatca gttacacaaa
     8761 tgctgaatga gatttctccc gatatcgtga gtgtctgtac acccaatcgc tttcacttcg
     8821 aacatgtatt ggctgccctc gaagctaatg cttcagtgat gtgcgagaaa ccaccagcta
     8881 tgacagctga tcaagcaaga aagatggcag agacagccga taaaaacggg cagattttgg
     8941 cttatggatt ccagcatcgt ttttctgacg aagcaacaat gatcaagaat catcttgatg
     9001 ttctcgggga tatttattac gtagaagcga atgcgcttcg tcggtctggt gttccaggct
     9061 ggggaacgtt tatcgacaaa gaacttcaag gaggcgggcc tttgatcgat tatggcattc
     9121 acatgcttga tacagcgatg tatattactg gatttcctaa agtaaaaagc gttcgggcgt
     9181 acagcttcca aaaaattggt acgaaaaaaa attcagggaa attcgggaaa tgggatccaa
     9241 gcaaattcac agtagaagat gcaatttttg gcacactgga attagaaaac ggcaagatca
     9301 tccaactgaa tacttcgttt gctttgaata tcagagaaga caaactgaat gtggcttttt
     9361 gtggcgatca ggcaggtgct aatctgtatc caggcgaact attcgaagat gtagatggaa
     9421 agctggtttc cttacaaaag accacgccag ttaatgaccc acatgaacta agtatggctc
     9481 attttgtgaa tcgggcgctc ggtgaccaga ctcagatgat tgcagatggc tggcaaggat
     9541 atgtcatcca gaatctggtg gaaaatttat atcgttcggc agaaacagga gaagttatta
     9601 ccttatgaaa gtagaagaaa aacagtttga tttagagtat atcaataagt atgcgagtca
     9661 gatgaccatt ggaaatggat tttttggtat cagaggaagc caggaggaag actatcggaa
     9721 tcaagtaagg ggaatgtttg cagctggtgt atataaccga cctttaggag cagaatcagc
     9781 cgagttagtg aatctcccag atgtcatcag atgggaaata aaaatcgacg gtgaacattt
     9841 ttctcttgag acgatgaaag ttcttgcata cgagcgggca ctagatttag aaatgggaga
     9901 gcttaaacgc tatgcgctca ttgaaactgc tcaaggaaaa aaagtggaag tcattacttg
     9961 tcgtgttgct gatcaaaata atctccatca ggcagccctg aaagtggtca tcaagccgtt
    10021 gaccgaagac tgccaagtag atgtgaaaac gggtatcgat ggacagcaaa ccaattttgg
    10081 tacacagcag ttggttgaaa aagaactgcg agtatttggt gacgatttga tcagtgcatc
    10141 ctaccagaca acagaaagta aaattgatat tgggattgca gctaaattca ataaagcagg
    10201 tgtattccaa gcaaaaaatc gccggatcac cgcttccttt tcagaaaaga tcagaaaaaa
    10261 tacgtccttt acgctggaga agatcatcgt gctctctacc tctcttcagc aaacagaccc
    10321 gtttaagaaa agtattgccc aagcccagaa aagcgttagt tatacagaaa ttttagagca
    10381 gtcgatgtct tattggacag atttttggca tcagcatcgg ataacgatcc aaggcgattc
    10441 ttctttcgat cagttagcga tggacttcgc tgcttaccat ttacagatta tgactccccg
    10501 caatgatcca agtgttagtg taggggcgaa gggactaact ggcgaaggtt ataaagggca
    10561 tgttttctgg gatacggaaa tttttatcct gcctttcttt ttatatcagt ttcctgaagt
    10621 agcaaagaat ttgctgctgt accgttatga tcgattagaa ggcgcacggc aaaaagcaag
    10681 agattatcac tatcaaggtg cacttttccc atgggaaagc gcacacactg gtcaggaaga
    10741 gacacccaaa tttgctgcat tgaatatcaa aaccggaaca agacaagaag ttgcttcagc
    10801 tgccgcagaa catcatatca gtgcagacct tgcttatgcc gttcaagagt atgtccaagc
    10861 aacagaggat caatcgtttt tagaaacaaa gggaaaaacg ctcattcatg aaacagcagc
    10921 tttttggctg agcagagctg taaaagtcga tgatcattta gagatacaca gagtcattgg
    10981 tccagatgaa tacacagaat atatcgacaa taatgcatat acgaactatc tggcgcatta
    11041 taatgtggcc ttagctgtta aatatcaagc tggcgatccg ctttttttga aagaatgtca
    11101 aaatttttta gataagttat atttgccagt gcctaacaaa gaacagctga ttccgcaaga
    11161 tgacacgtat ctcagtaagc cagttattga tttgaccact tacaaagaac gtcctggcag
    11221 ccagtctatt ttacttgatt atgcccgtag cgaagtaaat gaactccaag tcagcaagca
    11281 agcagatctt gtcatgctgt tatacttatt acctgagtat ttttctcaac caatcgtttc
    11341 taagaatttg cattactatg aaaacaaaac aatccacgac tcatcgttga gtaaagcgat
    11401 tcacgccata gaggcttgcc gtatccaaga aatggaatgg gcgtatcatt tattccaaga
    11461 agcgtgcctg attgatttag gaccagatcc gcattcatca gatgacggaa ttcatgctgc
    11521 ttcattagga gcactatggc tagcagttat atttggtttt tcaggtatta cagtaacgaa
    11581 tggaactctt tctatttctc caagacttcc ttcagcatgg gaaaaaattt cttttccttt
    11641 tatttggaaa aatcacaggt tgctatttgt tttgacaaaa aaacaaatag taataaaaaa
    11701 agagacgaaa gaaacattac ctatttttgt caatggagac caataccagc tggaaaaaga
    11761 actgatcttg gaacggaggg gaaaataatg cgtattctga ttgcgccaga ttctttcaaa
    11821 gaaagtctga cagccttaga ggcagcacaa gccattcaat caggttttca agaagttttt
    11881 gttcaggctg aatatgatct gctacctata ggcgatggag gagaaggaac ggtttcatca
    11941 ttagcagaca gtttaaggtt gaagcggcaa acagcagtcg ttactaatgc atttggcaaa
    12001 aacaaagaaa tccagtacgc ttcagacggt caaactgcga tttttgaaat ggcagaaatc
    12061 gtcggattga aagatgtccc actaaaccaa caacagccac ttaggttatc cacgcgagga
    12121 gtaggagaac tgatacgaca attgttgcaa caaggtgttc gacacatcat catcggcgtt
    12181 ggcggaagta gtaccaacga tggcggcatc ggcatggcag aaggtctagg ttatgctttt
    12241 tacgatcaag ataatcaaaa agtagaagca ctgggagaaa atctaggtaa aattgctaca
    12301 gttgataaaa cgaacgtgcc tattgagctg attaaagcca aaatcactgt cattactgat
    12361 gttactaatc ttttatgcgg aaaagaaggc gcttcttttg ttttcggacc acaaaaaggc
    12421 ttgaaagaaa atgaattagc agtggctgac caagccatgc atgattttta tatgagattt
    12481 gcccctcaag taatcgaggc atcgggcagt agtgctggtg gtggaatggg tgcaggactg
    12541 ttagcatttg cttcagggaa aatagtctct ggcatcgact acgtattgga tttactggag
    12601 ttcgatacgg gtacagcaag cagacctcgt aatcgtaggc gaaggacgtc tagatggaca
    12661 aagtctaaaa ggaaaagcgc cagttgggat tgctagaaga gtaccagagg gcgttcctgt
    12721 gatagctatt tgcggaagtg ttggtaaagg aagcgaaaaa gcagcagatg ttggcatttc
    12781 ggccgttttt ccgatccttc ctgcagctgt gcctttacaa gaagctttac agcaagccac
    12841 tgataatctt catcgcacag ctaaaaacat cgcatcttta cttcaagtcg ttcattttca
    12901 aggaggtaat cgatgaaaat caaagcagca ctattcgatt tagatggcgt tcttgtagac
    12961 acagctcgtt atcattacga agcgtggctg gttttagcta accagctgtc cattccattc
    13021 actgagaaag aaaacgagca actgaaagga atatccagaa ccgagtcact agagcgattg
    13081 ctgtcatttg gaaaaatgga acagaaattc tcagaaaaag agaaatcagc ctttgctgag
    13141 caaaaaaaca atctatatct tcaagcgatt caaaaaatgg atgaaacatc cgttttgcct
    13201 ggtgcaatag ctgttcttga gtacttgaaa aaaacaaaca tcaagatcgg attgggatca
    13261 gcaagcaaaa atgcacgcct cattttagaa aaaacgaatt tgacaagtta ttttgatgtc
    13321 cttattgacg gcacgcaagt atcaaaagca aaacctgatc cagaagtttt tctcaaagga
    13381 gcacagcaat tgaatgtacc gccaaatgca tgcttggtca ttgaagattc agaagctggt
    13441 tgtcaagcag ctttggctgg taacatgcat gtattgggaa taggtgaaaa cataaatctt
    13501 ccttctgctg aatatgtgat tcctgattta accgtttttg atcaagtacg ttcgttttgg
    13561 catttatcag aagccgtatc acgtatataa tagtttccgt tttttctgtc acaagcaaaa
    13621 cgttatcctc cttttggaaa tcaaggtatg atgagaaaaa agggggattt tatatgacaa
    13681 aaaaagcagt tttcgcaggc ggatgctttt ggtgtatggt caaaccattt gaagagcagc
    13741 cagggatcat ttctgtgaca tccggttata ctggcgggca tgtaccaaat ccaacttatg
    13801 aacaagtaac aacaggcaca acagggcata cagaagcagt agagatcgaa tatgatccag
    13861 aagttatttc ttatgatcag ttggtggaaa tctattggca gcaaaccgat ccgactgacg
    13921 cattaggaca gttcgctgat cgcggtgatt cttatcgtcc agtgatctac tataatgatg
    13981 aagaagaacg aaatattgca gagcgttcaa aacaagcatt gcaagacagc gggcgattcg
    14041 ataagccaat cgtgacaaaa atcgaaccaa gaagcgaatt ttatcctgca gaagattacc
    14101 atcaggatta ttacaagaaa aacaagctcc attacaatct ttatcgagaa ggttctggac
    14161 gtgcaggttt ccttcgtcga cattggggcg tcaagaaata agaagccgaa aaagctactt
    14221 atggaacacc gtatatcaga ggttgtaaaa ccagcatttt gagctaatac tgagggtcag
    14281 ctgattgaac accatttagt ggagaggttg tgaacctgtc gcctagctcc aagtcacaag
    14341 gatttggtaa gagaacacta tatatcagag gttgtgacaa gtcgtttgtc acaacctttt
    14401 ttcaatacct aagatgaagg acaatcagca acgcactgct caaaaaagga atgaaaggca
    14461 gcttttccaa ctttttttta tgacagataa agtaaacaga aaaaacggat attccaaaca
    14521 gactagcaag aaacaagatc tgaagcaact cttctagaga caagccacca ctccaagaaa
    14581 gcatcaacag agtatcgcca gctccgagat aggattgacc atagcgaagg cacacaccgc
    14641 ctacgataaa cgttaacagc acactttgcc attggaagac gccggcctgc aaccaaaaaa
    14701 tccataaaat tgcccaagaa cagaatagcg tgtgagaatg gaccatgaag taaaataaat
    14761 ccatcaacga aaatagcaaa gccgaaagca tccatatgaa cgtcgaaaaa tttcttgcgg
    14821 aagaaaaagt aaagaaccag gcaaaaagac caccacataa ggcttctgcc aacaaatagt
    14881 agaagggcaa ccgacacctg caatagcggc agcgaaatcg ttggacgatg atcgagagaa
    14941 tcggaatcaa ttcataccaa gaaagcgaac gacagcattt gacacaatga gatctaggat
    15001 aaataataga gtgacctaac ggaatccgct gggcggttaa acataaaaat gatccgatac
    15061 agcaaccaat gataaaataa agtaacacgc catctccccc ttttcaaaac caagatacgc
    15121 aagcttaaac taaatttttg tacatacata aaatttgagg tgaaaaaatg aacgtttcaa
    15181 tcggattaga tatcggaacc acacaaacca aagcagttgc tttcacagat gaagcccaag
    15241 aagcagcatc cgcttatttt cgctaccctt tgatccaaga aacacaaggg atggcagaac
    15301 aagatcagga attgatcttt caaggtgtct gcaccgttat aaaagaagta attaaacagt
    15361 tgccttcaga cacaatcatt caatttgtct ccttctcctc tgcgatgcat ggcctgatat
    15421 tgttggatga agagaaaaca ccgttatctc gaatgatcac ttgggcagat aaccgagcag
    15481 cggacgaagc agaaaaactg aaatataccg tgcttggcaa agaattttat cggcagactg
    15541 ggacgccgat tcatccaatg acaccattga acaaaatcaa gtggctgcaa aacagccaac
    15601 ctgaattatt caaaaaaact gcttattttg tcggtatcaa agactatatc ttttatagat
    15661 tgtttggcga actgatcagc gactacggaa cagcttcagg aactggatat tttgatatcc
    15721 atgaattcca atgggcagaa gaagtgcttg actacttgaa cattcgaata gagcaattgc
    15781 cgcaagtctt tccttccacc tatcaagtaa caggtctacc atcaaaaaca gcagcagaac
    15841 taggtttgcc aaatgatata tcatttgttt tgggaggggc agacgggccg ctttctaatc
    15901 tagggttagg tgcttttggt gatacgatcg ccgctttgac cgtaggaacg agcggagcac
    15961 ttcgttttat tactactcag cctcagttcc atccagaaat ggagacgttt tgttatgttc
    16021 tggatcagaa acattgggta gtgggaggag cgactagcaa tggtgctggc atctttgatt
    16081 gggcagggca tacattttta caggaagtcg ttcaatctgc taaagaaaaa ggagaaaatc
    16141 catatgatgc actgctttcg gctatcgctt ttgtgccagc gggtgccaat ggtctgattt
    16201 ttcatcccta tttacttggt gagcgagcac cgctttggga cgcagatgcg tttggcagtt
    16261 ttatcggttt acagcggcaa catacagaaa aagaaatgat gaaggcgatt ctagaaggga
    16321 tctgtctcaa cctttatcgg attttaaaag gagtttgcca ggaaaatcag ctcacagaga
    16381 tccgtgctac aggcgggttt gctcaatctc cgatttttcg tcagatgatg gctgatgtac
    16441 tgggttaccc gcttgttttc cctactgtca cagaagcatc cgcattagga gctgtgattt
    16501 taggctggag aagcatcgga aagatccaaa gtatcagtga agcacaggaa ttgatagaat
    16561 tgaaagagca agttggagta caagaaactg atcataaaaa atatcagaaa atctatccat
    16621 tatttgtttc gacacaaaag catttagcac agacttatca gcaatttgct caactgcgag
    16681 aagaattagc tgattctgaa aaatgattgg ctttacggat aaaaaagcga actgaaaatc
    16741 gttctcaggt aaatgatttt gttgtacttt tcattgaaag tctactatta ttttaagtgt
    16801 aagaaaatgt taggaggcaa gaaagatgaa attccaacaa accaaagaag tattaaatca
    16861 attagttgca gatctaagcc aaatgtctgt tgttgttcac caaacacatt ggtacatgag
    16921 aggaccaggt ttccttacat tgcacccaat gatggatgag ttcatggatg atttgaacgc
    16981 tcagttagat gaagtatccg aacgcttgat cacattagac ggttcacctt attctacatt
    17041 gcgtgaattt gcagataaca caaaaatccc tgatgaaatt ggccgctggg atcgtactat
    17101 tccagaacgt ttggaaacac ttgttgcagg ctatcgttat ctagcagatc tttaccaaaa
    17161 aggaatcgaa gtttctggag aagaaggcga cgatccaaca caagatatct ttatcgaatt
    17221 caaaacagcc acagaaaaac gtatctggat gatccaagct catttgggca aagcgccaga
    17281 gatcgacgcg taaaaactgt tgttgaactt gaccgaaaaa gaggttgtga aatcagcgtt
    17341 ttgacctgag tattagagaa acaaacgtca aaatagttcc tcatattttg ccgttttttg
    17401 agctaatacc gagggtcagc tgattgaaca ccgtttacca agaggttgta agcctgccgt
    17461 tcagctccga gtcacaagaa acaattttaa aaaacagctt cttatgtttt atggatattg
    17521 tgacttgtga ccgaggagtt ggcaggagaa caccgtttat tcaaaataag gggttgtgac
    17581 aagacttttg tcacagcctc tttttgtttt tcattttgac aggaaacgac ccttttatat
    17641 aaaagaaaga aaagaagtaa agtttcatat aaatttttca atcaagcaga aaaacgttga
    17701 cattgactag attagcgggt aatatgttaa atggtattgt ttgccccaca aagagccccg
    17761 gaaactcgaa gtgtatgtca aacatcagaa cttaaattgg aggaaaataa cgtgcgtaca
    17821 acatatatgg ccaaaccagg cgaagtagaa cgtaaatggt atgtagtaga cgcaactgat
    17881 gttccattgg gacgtctttc aacagttgtt gcatctgtac tacgtggtaa aaataaacca
    17941 actttcacac ctcatttgga tactggcgat ttcgtcatcg tcatcaatgc agacaaagtg
    18001 aaattaacag gtaaaaaagc gactgacaaa atttattacc gtcacagcaa ctacccagga
    18061 ggattgaaat caatcactgc tggcgaattg cgtgctaaaa attctcgtcg tttgatcgaa
    18121 acttctgtaa aaggtatgct tcctaaaaac actttaggcc gcaaacaatt tacaaaattg
    18181 aacgtatacg gtggagcaga acatccacat gcagcacaac aaccagaagt tttagacatc
    18241 acaaacttaa tctaatagga gggaatttca ttggcacaag ttcaatatat cggcacaggc
    18301 cgtcgtaaaa acgcagttgc tcgcgtacgt ctagtaccag gaactggcaa aattactgta
    18361 aataaaaaag atgttgaaga atacattcca catgctgact tgcgtgaagt aatcaatcaa
    18421 cctttcgctg ttactgaaac aaaaggcgca tatgacgtat ttgttaacgt aaacggcgga
    18481 ggatacgctg gacaatcagg agctatccgt catggtatcg ctcgtgcatt gctagaagtt
    18541 gatcctgact tccgtttagc tcttaaacgt gctggcttac ttactcgtga cgcacgtatg
    18601 gtcgaacgta aaaaaccagg tcttaagaaa gcacgtaaag cttcacaatt ctcaaaacgt
    18661 taatatcctt tatatatcaa cgtttctggc acttttcaat tatttggaaa gtgcttcttt
    18721 tgtttttaag aacacattta agaacacata tcaatcacac ttattcaatc ctctctgaaa
    18781 agcttcaaaa acagccttat caactatatc tggtgtgttc acatatatct ttgttgtaga
    18841 aatatctgag tgtgttaaag catttgaaat ttgattcata tctgctccac cttgtctagc
    18901 tatagttgca taagtatgac ggagtttgtg tggagataaa tgaactaagt caggatgcct
    18961 tcttttaatt gaattgattc gatagtttag gtaatcggca tgaacaggac aatttacttc
    19021 tccagatggt ttagtataag taaagagata ctgatctgac gtttgcttaa tttttaattt
    19081 caacaattga tcagcttgta ccgatttcca ttcacttagc aatgtcatta caacttctgg
    19141 tacttgaatg acggtatctt ttcgaccttt tgtaaacttt tttctttgat atttatctaa
    19201 agtatgttta agacggaccg tttgtttttc aaaatcaata tatttccatt gcaaagcgta
    19261 agtctcactt tttctatcac caagatacag ggtaatcata aaaagtgtgt aatcatgaaa
    19321 agtaagtaaa tgattattaa aatcatcatt tactgcttct atccaattag taagttcttt
    19381 tgatgatagt gcttgcttac cttcttgtat tcttttttct tctaaagcat tttttttagg
    19441 tgcagtgata ctttgaataa ttttagtagt tcgattaaat tcgatataat ttagtatttc
    19501 tgcaatatcg aacatggaac gaacatatcc ttttatgatt tttatatttg catattcttt
    19561 cgattttttg gttaattcat ctgatattat ctcagtattg atatttagat aattcattgc
    19621 gtattcacca aacataggta aaatatgcaa ccgaaatata tcttttgtat tactaatggt
    19681 tatatcacta ggaggcctgt ttgatctaat tgtttgacct aattcatatc taggaagcca
    19741 tttagattcg taaaattttt taaacgttat ttttcctttt aattctaatg agtttgcatt
    19801 ctcctctctc aaaacttttt ctattttctt cttcatatca ttttcggctt taatcgccaa
    19861 ttgcctattt ggaaatgttt tttgatactt ttctgatgca attcctgtta tttctttaaa
    19921 cctttttgga tatctcagat tagttttata tttcccattt gaaagctttg taggcaaaat
    19981 cctcactcct taaaaaaagt aaggcagtat atttcactaa ttttagtata tgcggcgaga
    20041 agaaaatttc aagttatttt aaatgtattc ataaagtctt taatatcttc tacgtcaaag
    20101 cgaactacac caccaactct tattactggg aaattatata tacgaatcca cttatctaga
    20161 gtattattat cgattcctag aaatgcacag gtttgttttt tattcaggta gactctggat
    20221 tctataaaat catctcctaa aatagtagtt atgaaatttt tgaattgctt tgggtaagca
    20281 cataggaatt tcacctctcc ccagtttacg gcgagcctta cggaagtatc attatttgct
    20341 gctattgtca tggcattttg ttttcccaga acaaaaagaa atctctaagt atttgtcgct
    20401 aatcaggtca tggcgtactt gagtttctag ctcgaatagc agggatcgtt attcaaaaat
    20461 tgtatttaat tgtcaatgat caaaaatagt ttttttccta ttcactatta ataacgataa
    20521 acatgtataa aaagacacac tacgaaaaaa gttaaatgga gtttactgat tttcatattt
    20581 gtatatggaa cagacatagt cttgaataat ttttataaat gttttgatgc ttatcaactt
    20641 attcaacccg ctatccactt caagtataat ttttccatac gccaataaag tagttttagg
    20701 agcatgttct catttgagaa cgctcctttt ttatatattt tgaaagctac ttactatacg
    20761 cgtagtttca aatagttttg tcattctaat cttcagcaac agaaaaaaat ctaaattata
    20821 aaatttaggg ttgactaaaa tataattttt gtataccata tgatcgtgat aatcattatc
    20881 atttagaagg agaaagttat gttagaagtg aaaaagctta atgtttgtta taacgatttt
    20941 ttagcattga acgagatttc tttaaaaata caagaaggtg ctttaacagg tattattggt
    21001 ccaaatggtg ctggaaaatc cacattgctt aaagcaatgt taaatattat ccctcatcag
    21061 ggagaggtct ccattaataa tgaagatatc aataaaaaac tggtgaagat tgcatatgta
    21121 gagcaaaaag ctgatattga ttttactttt ccaataaaag taaaagaatg tgtatctatg
    21181 ggaacatatg ccggaatgaa agtttttcaa agaattaaaa atgcagaatg gcaaagagtt
    21241 tcaaaggctt tggaaaaggt cgatatggga aaatattcta atcatcaaat aggagaatta
    21301 tctggaggac aatttcaacg tgtactttta gcaagatgtc ttgctcaaaa tgcggatttc
    21361 atatttttag atgaaccatt tgtagggata gacttagtta gtgaacgaat aattatggat
    21421 acgttaaaaa aattgaaaca tcagggaaaa acagtgctaa ttgtgcatca tgatttaagc
    21481 aaggtaaaga agtattttga taatattatt cttttgaatc gaaatctaat tgctcacgga
    21541 agtgtagaat ctgtatttaa tgaagaaaat ttaaaaaaag cctatggaga tactatattt
    21601 attggagagg gggaataata tgcttcaaaa ttttattaat ggtctgtatg actttcactt
    21661 tttacagaat gctctaatta catctgtagt tatagggatt gtttctggaa cagtaggttg
    21721 ttttattatt ttaagaggaa tgtctcttat gggtgatgct atttctcatg ctgttttgcc
    21781 aggagtagca atttcttata ttttaggtat taattttttt attggtgcga ttgtatttgg
    21841 tctattagct tctatcatta ttacttttat aaaaagcaat agtgtaataa aaggagatac
    21901 agcaattggt attactttca gttctttttt agcactagga gttatcctta taggagttgc
    21961 aaatagctca acggatttat ttcatatttt atttggaaat attttagctg tacaagatat
    22021 tgataagtgg attacaattg gcgtttcggt tttagtgttg gttatagtag ttctattttt
    22081 taaagaatta ttaatcactt cttttgatcc attaatggca aaagcaatag ggatgaatgt
    22141 cagtttttat cattatctat tgatggttct attaacttta gtagcagtaa cagctatgca
    22201 aagtgtggga actatattaa ttgttgcaat gttaattaca ccagcagcta cagcatattt
    22261 atatgcaaat agcttgaaaa ctatgatatt tatttctgca acagtcggag caagttcatc
    22321 attgcttgga ttgtttattg gttatagttt taacatagca gcaggatcaa gtattgtttt
    22381 aacagcggca ttcttatttg taattagttt tctaatctca ccaaaacaaa aatttttaag
    22441 aaagaaggag agatcgttat gatgagaaaa tggaaagcag tactgggaag tctggggatt
    22501 ttgattgctc tttttatatt cggggcatgt tcaataaata gtaaagacaa agataaagtg
    22561 gcttcgaacg aaaaattaaa agtagtagtt actaattcga ttttagcaga tattaccgaa
    22621 aatatagcaa aagataaaat tgatttacac agtatcgtac ctattgggaa agatccccac
    22681 gaatatgaac ctttgcctga agatgttcaa aaaacttcaa aagcagattt gattttttat
    22741 aacggtgtta acttggaaac tggaggaaat gcttggttta caaaattagt taaaaatgcg
    22801 aacaaagagg aaaacaaaga ctattttgca gcaagtgatg gcatagatgt tatttactta
    22861 gagggtcaga gtgagaaagg gaaggaagat cctcatgctt ggttaaattt agaaaacggt
    22921 attatttacg ctaaaaatat tgaaaaacag ttagcggaaa aagatcctga taataaaaaa
    22981 ttctataaag aaaatctaga taagtatatt gaaaaattgg attctctaga caaagaagct
    23041 aaatctaaat ttgcttcaat tccgaatgat aaaaaaatga ttgttacaag tgaaggatgc
    23101 tttaaatatt tctcgaaagc gtataatgtg ccttctgctt acatttggga aatcaacact
    23161 gaagaagaag gaacaccaga tcaaataaaa cacttagttg aaaaattacg cacaacaaaa
    23221 gttccctcct tattcgtaga aagcagtgtg gatgatagac caatgaaaac agtatcaaaa
    23281 gataccaata ttcctatcta ttcaacgatt tttactgatt caattgcaga aaaaggacaa
    23341 gatggtgata gttactatgc gatgatgaaa tggaacctgg ataaaattgc tgaaggcctt
    23401 tcgaaataat ttggagggta ggtatggtac aaattaaaac gactactata ctaatttatt
    23461 tactagcaat tcaggaatta tcaaaaagaa aaaagaaatt aaaaaataat gatttagcaa
    23521 aagttctaag tgttagtcca gcatctgtaa gtgagatgct ggctaaactt agatatgagg
    23581 actatttaga tataggattt caattaactg ataaagggaa aaaatttatt gacgattaca
    23641 aaaaaggttt taagtaaaag actttatttt tagggaggat aagaaaatgg ctattggagt
    23701 gattatgata ggacttatcc taatagctgg atataatttt tttaaatctt tctcggatta
    23761 ttttagaaaa ggtgaatgat ttagatgagt ctattaattc ttgtaggatt gctttttgta
    23821 tcagttattc tgctctttct cattagttta gctctaagga atgagttcag taataaacgt
    23881 taacgattga aacttcaagt cctgtgtaag ataataaagt taattcatct tattaggagg
    23941 aagtacccat agaaaccaga gattatatta taactcacca aaagttaata gaaagtggac
    24001 taagccattt tgaaaaagaa ggatttcagc aaactaagat aaggcaactg tgcctgaccg
    24061 caggcgtaac tacgggtgct ttttataaac attttacttc taaggaagcg ttatttgaag
    24121 aaattattca gccgcatatt aatgcgctta t
//
LOCUS       DAXWOO010000027        23990 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.027, whole genome shotgun sequence.
ACCESSION   DAXWOO010000027 DAXWOO010000000
VERSION     DAXWOO010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23990
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.027"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(216..1511)
                     /gene="purB"
                     /locus_tag="ACGE70_000855"
     CDS             complement(216..1511)
                     /gene="purB"
                     /locus_tag="ACGE70_000855"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFF8692508.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1524..2648)
                     /gene="purK"
                     /locus_tag="ACGE70_000856"
     CDS             complement(1524..2648)
                     /gene="purK"
                     /locus_tag="ACGE70_000856"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFF8692509.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2641..3129)
                     /gene="purE"
                     /locus_tag="ACGE70_000857"
     CDS             complement(2641..3129)
                     /gene="purE"
                     /locus_tag="ACGE70_000857"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFF8692510.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3545..4852)
                     /locus_tag="ACGE70_000858"
     CDS             complement(3545..4852)
                     /locus_tag="ACGE70_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFF8692511.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4855..5436)
                     /locus_tag="ACGE70_000859"
     CDS             complement(4855..5436)
                     /locus_tag="ACGE70_000859"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFF8692512.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5552..5649)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(6008..7300)
                     /locus_tag="ACGE70_000860"
     CDS             complement(6008..7300)
                     /locus_tag="ACGE70_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFF8692513.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7297..7920)
                     /locus_tag="ACGE70_000861"
     CDS             complement(7297..7920)
                     /locus_tag="ACGE70_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692514.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7923..8759)
                     /locus_tag="ACGE70_000862"
     CDS             complement(7923..8759)
                     /locus_tag="ACGE70_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8692515.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8776..9663)
                     /locus_tag="ACGE70_000863"
     CDS             complement(8776..9663)
                     /locus_tag="ACGE70_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8692516.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9679..10962)
                     /locus_tag="ACGE70_000864"
     CDS             complement(9679..10962)
                     /locus_tag="ACGE70_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFF8692517.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRIKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11645..12328)
                     /locus_tag="ACGE70_000865"
     CDS             complement(11645..12328)
                     /locus_tag="ACGE70_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFF8692518.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12346..12963)
                     /locus_tag="ACGE70_000866"
     CDS             complement(12346..12963)
                     /locus_tag="ACGE70_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFF8692519.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12973..13797)
                     /locus_tag="ACGE70_000867"
     CDS             complement(12973..13797)
                     /locus_tag="ACGE70_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFF8692520.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGTKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13790..14350)
                     /locus_tag="ACGE70_000868"
     CDS             complement(13790..14350)
                     /locus_tag="ACGE70_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692521.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14524..15480
                     /locus_tag="ACGE70_000869"
     CDS             14524..15480
                     /locus_tag="ACGE70_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFF8692522.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15785..16741)
                     /locus_tag="ACGE70_000870"
     CDS             complement(15785..16741)
                     /locus_tag="ACGE70_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFF8692523.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16845..17447
                     /locus_tag="ACGE70_000871"
     CDS             16845..17447
                     /locus_tag="ACGE70_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFF8692524.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17535..19034)
                     /locus_tag="ACGE70_000872"
     CDS             complement(17535..19034)
                     /locus_tag="ACGE70_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFF8692525.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(19050..19574)
                     /locus_tag="ACGE70_000873"
     CDS             complement(19050..19574)
                     /locus_tag="ACGE70_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFF8692526.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19872..20621
                     /locus_tag="ACGE70_000874"
     CDS             19872..20621
                     /locus_tag="ACGE70_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFF8692527.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20612..21397
                     /locus_tag="ACGE70_000875"
     CDS             20612..21397
                     /locus_tag="ACGE70_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFF8692528.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21366..22082
                     /locus_tag="ACGE70_000876"
     CDS             21366..22082
                     /locus_tag="ACGE70_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFF8692529.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22184..23203)
                     /locus_tag="ACGE70_000877"
     CDS             complement(22184..23203)
                     /locus_tag="ACGE70_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFF8692530.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(23222..>23990)
                     /locus_tag="ACGE70_000878"
     CDS             complement(23222..>23990)
                     /locus_tag="ACGE70_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFF8692531.1"
                     /translation="FLSYKGDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIF
                     NIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTG
                     AEGEISQVAFLRIDQTAPWLFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSW
                     YYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDR
                     EALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
BASE COUNT         7068 a         5190 c         4212 g         7520 t
ORIGIN      
        1 tattcttcat cgatcattaa cagacaaaga actggcccgc caaggatatg aggacatctc
       61 cttgaagtac cagttcgtac actcaactta ttgaaccgcc gtatacgggt ccgtacgtac
      121 ggtggtgtga gaggggcgga aggtcagttg gctttcccct ctactcgatt atttttgcta
      181 ttctatttta attgaatttt gcaactcact cttctttatt ccaaacctac tcgtttgaat
      241 atcacgtcaa cgtttttcaa atgatgatga taatcaaagg catcgtctat ttcttcttta
      301 gataataccg atgtgatggt tggatcttct tctaacagcg ggcggaactg cacttggttg
      361 tcccaagcgt acgcagtttt tggctgaaca agatcataag cttcttctct tgtcattccc
      421 ttatcgatca atttcagcat aacgcgctgg ctataaatca aaccaaatgt tgcagacata
      481 ttccgcttca tgttttcagg aaagaccgtt agatttttaa cgatattgcc aaaacgattt
      541 aacatgtaat tcaataaaat cgtggtatca ggcaagatga ttcgttctgc tgaagaatga
      601 gaaatatctc tttcatgcca taatgtgaca ttttcatagg cagtgaccat gtgtccgcgc
      661 accactctgg ctagaccagc catattttct gaaccgatcg gattcctttt gtgcggcata
      721 gcagaagaac ctttttgacc tttcgcaaaa aattcttcta cttcacgtgt ttcggatttt
      781 tgcaatccgc gtatctctgt tgcaaaacgt tcaatacttg tagcaataag cgccatcgct
      841 gagaaatatt cagcatgtag atcccgaggg agaacttgag tagagatttc ttgtggacgg
      901 atccccaatt gtttacatac atactcttca ataaatggcg ggatatttgc gaacgttccc
      961 actgctcctg agatttttcc tgcttctact ccttttgccg catgttcaaa tcgttcgatg
     1021 ttccgtttca tttctgaata ccatgtggca agtttcaatc caaaagtagt tggttcagca
     1081 tggacaccgt gtgtccgacc catcatcact gtatatttgt gttcttttgc tttttcgcca
     1141 atgatttccg taaaacgttt caagtcttcc cgtaaaatat catttgcttg cttgatgaga
     1201 tagccataag cagtatctac gacatccgta cttgttagac cataatggac ccattttctt
     1261 tcttctccca atgtttcaga aaccgctcgt gtaaacgcaa cgacatcatg gcgtgttaat
     1321 ttttcgattt ccaagatccg atcgatatca aatgatgcat gctcacgaat ttttttcaca
     1381 tcttcttgcg ggatttcacc aagttctgcc caagcttcat ctgctaaaat ctcgacttcg
     1441 agccacgcac ggtatttgtt ctcttccgtc cagatattcc ccatttcagg gcgtgtataa
     1501 cgttctaaca tgttttttct cctctaatcc caaatattcg tttgtttgat ttcttccagc
     1561 gtttgctgga tgtcttctgt caagatagtg atatgtccca tcttgcgacc tttttttgct
     1621 tgtttttttc catagaaatg gaattgccag tctggcttat tctcaatcaa gcgatagctt
     1681 tccagcagtt gttctcccaa aacattgacc attactgcat tgcttatcaa acgtactttt
     1741 tctcctaatg gccaaccaca aatacctcga atatgggcat cgaactgact catagaacaa
     1801 gcttcaatag aataatgtcc agaattgtga ggacgaggag ctaattcatt cacatatagc
     1861 ccgcctgttt ttgttaaaaa catttctacc cctaaaacac ctgaaaggct cactgcttct
     1921 gcaatcacac gggcaatacg ctcagcctcc tcgatcacat cctgatcaat cgcggctgga
     1981 gcaatcgttt catgaagaat attgttgtgg tggatgtttt ccacgaccgg aaaagtcgtg
     2041 aaatctcctt gaccatttcc tgccaccatg atcgaaatct ctttttcaaa cgggatccac
     2101 gcttctaaaa cacaggttcc ttctcttaaa aggtccatgg caggagctag atccgcccga
     2161 cttttcaaaa catactgccc cttgccatca tagccgcctc gagtggtttt caaaacacaa
     2221 ggatagccga tcccgtcaat cgcatcttgg atatccgtcg gactgacgat cgttgcataa
     2281 ggagcaatca cgatgttatt cgtttccaaa aaagattttt ctaacaggcg gtcttgcgta
     2341 atcgcaagca agtctgtccc ttgaggaata aatgacatgg gtaaaatcgc atttaacgcc
     2401 tctacactga cgttttcgaa ttcataggta atgacatctg ttcttctggc aagctctttc
     2461 agcgcaaagg tgtcatcata gacactttca atgtgccaat ctgcaacttg tgcggctgga
     2521 caatcgctca caggatccag cacacctact ttaaagccca tttcttttgc actaatcgcc
     2581 atcatacgac ctaattgacc gccaccgacg atcccgatcg ttgaaccagg caacaaaggt
     2641 ttaaccaagt tgatcactac tttctataac agttttagcc aacattgaac gtctttcagc
     2701 caatttagcc tccaattcta aatcatacat ggaaagcatc tgtatcgcaa gtagtccagc
     2761 attaattgct cccgcttttc cgattgctgt agtagctaca ggtactcctc caggcatttg
     2821 gacaatcgaa agaagcgaat ccaaaccatt taatgttcgt gattggaccg gtacaccgat
     2881 aaccggcaaa gttgtttttg atgcgaccat acccggcaga tgcgcggctc cccctgctcc
     2941 tgcaataatg attttgattc ctttttttcg agcgttttgt gcatattcaa acatcaaatc
     3001 tggggttcga tgagcagaaa cgaccttttt ttcataagac acaccgaatt catccaatat
     3061 ttgacatgct tctttcattg tttcccaatc tgaagtactc cccataatca cagagatttt
     3121 tgctgtcatt gtttttcctc ccagtttttc tacattcata ttttaacaat cacttttttt
     3181 gatgtcaaag aaatttcgaa tgttaacaaa gaaaaacatt ttattgttcg tgtttattaa
     3241 agataaaaaa aaggaatgtg acaaaagtta ggtcacattc ctttttatgg ataaacggtg
     3301 ttctcctgcc aactcctcgg tcacaaggaa caatattcaa caaacatgag aagctttttt
     3361 ttgaaattgt tccttgtgac tcggagatga acggcaggct cacgacctct tccttcaacg
     3421 gtgttcaatc agctgaccct cggtattagc tcaaacaaac ggcaaaatac gaggaactat
     3481 ttttccgttt gtttctctaa caatcaggtc aaaacgctga tttcacaacc tttcttttgc
     3541 tctattactt cttgaaggct tcttccaata tttcttcatc ttttttcaat ccattaaaga
     3601 tcaaattcaa tatgatagcc gtaatgctac tcattacgat gccatttcca gtgaacattt
     3661 gcaatgtttg cggcatttga ctgaataatg tcggcgtaac attgaaaccc agcccaaatc
     3721 caacagaaac agcaacaatc aatagatttt tatcattgct gaaatcaact tccaacagca
     3781 tacggattcc ttggactgcc accatcccga acatgactaa cattcctcca ccaagtaccg
     3841 gctcagggat gatttgtgct aacgcaccga ttttagggaa taatcctaaa acaatcaaga
     3901 agaatgcaga gaaatagatg ggttttcttg ttttgattcc cgaaagttga accaatccga
     3961 cattttgaga aaagccagta taagggaatg tattgaaaat accgcctaag ataacggcaa
     4021 gtccttctgc acgatatccc tttttcaatt cttcttctcc aacatttttc ccagtaatgt
     4081 caccaagtgc aaaatataca cctgttgatt ctaccatgct gaccatggaa atgatgatca
     4141 ttagtaaaat cgaccatacg tcgaaagttg gtttaccaaa ataaaagggt tgtggaacat
     4201 ggaacaacgg cgcatctcca atcgcgctca gatcaatcat tcccatgaaa gcagctaaga
     4261 tgcttcctcc gactaaacca atcaataccg cgattgaacg aatgaaccct tttgccagca
     4321 tttgcacgcc tacaattagg agaatcgtca caaatgcaag tagcagattt gttttgtcac
     4381 caaaatcagc tgctgttgca ttacctccac ccattttctc gattgctaca ggaatcaaag
     4441 tcaaaccaat aacggtgata accgttcctg ttactatagg agggaatagt tttttaattt
     4501 tagaaaagac accagaaact aacacaacaa agatcccaga agcgataata gatccgtaga
     4561 tagctcctac tccttcattg gttccgatca agatcagtgg agctactgct tggatcgcac
     4621 atcctaaaac gacaggcaag ccaattccga aaaatcggtt aactgtcaat tgcaataatg
     4681 tcgctaatcc acacataaag atatcaattg aaatcaaata agtcatctgt tcttgattaa
     4741 agcctagtcc tgtaccgatc aaaagaggca ccgctactgc tcctgcatac atcgctagca
     4801 gatgctgcag ccctaaaacc gcagcttttc cattttgtgt ttctttttcc acgattatgc
     4861 gtcctcctca agaaactcaa cttgtccatt cgtcaaggaa gcaatacgcg caagagaaac
     4921 gactctcaat cccatttctt caagtaactg acgtccatct tggaaagact tttcgatgac
     4981 gatcccgatt ccttctactt gcgctccagc ttgctggcat aattcgatca atccttttgc
     5041 cgcttgtccg tttgccaaga aatcgtcgat aatcaatact ttgtcttctg aagataaaaa
     5101 tttacgagaa atagaaatcg tacttgtcac ttgtttcgta aaagaataaa cagaagctgt
     5161 caataattct tcatccatcg tcaaactttt tgcttttcta gcgaagatca ttggaacatc
     5221 taattctctt gcagcaaata aagctggtgc gatcccagaa gcttcaatgg tcacaacttt
     5281 cgtgattcct tgttctttaa atacttctgc aaaccgtttt cccatttgtt ccatcaatac
     5341 agggtctact tggtgagtga cgaagctgtc gacttttaat acgccctcgc ctaatatacg
     5401 tccgtctttt tgaatacgtt caactaattc tttcacttta tttgctcctt tatcatttat
     5461 taataccgaa ataaccgctt ttgtgcaaaa acctactatg tttctgttaa agaaaaacac
     5521 cctccacttt atcataagaa aagaagggtg aaatagccaa tcttttactt atagtccagc
     5581 atttacggta ctggctagaa actgtcatcc atattatgac gatatataag taggtatgta
     5641 aaactaataa tagtgtaaat tcattttctt gcaactgttt tgcacgtgaa ggcagtttgc
     5701 catatttgaa taaaaaatgt tgtttcaaat attgaaaaca gtctaacact actttctgaa
     5761 actgacaacg tggtttactt atttttttgt aaaatctaat gatttttaat gcattattcc
     5821 tttttgttcg ggtttaaata gttattttta ttttctccta tctaaaaaag cagcccacat
     5881 aaaaagaagg catgacaaaa agcatgtcat agcccctttt tccgaataaa tagtgttcaa
     5941 aagacagcct atcggcaata aggcaagatt ttataaacta cgggaagtaa tttccgtaaa
     6001 tctattctta ttcttttaag ctgacaagct cgtcaatcac tactgctttt cctgaagcaa
     6061 tagaatgatt tgctgcgatt cctgtcaaaa tcgacaaaat accatctacg tgagttgctg
     6121 cccgattcca cggatcttct tctttttctt caccaaatag atcatctaat aaacgcggat
     6181 ctcctccgcc atgtccgccc gcttcttgtt cgacgattac ctcatatggc tcaccgaaca
     6241 tcggatagac atggatggac tgaagaacgg ctgctccttc atccttttta tctccacctg
     6301 cattgatgta agacttttcg atcactttca tttcgatccg tcctttgctg ccattgaaag
     6361 aaacattgaa tccttcccac ggcatatacg cgtttagcga atacgtcaaa atcgcaccag
     6421 tttcatattt gaccgttacg cctagtgtat cttcaataga gatatcatcg gaaaatacac
     6481 tgcggtcacg aatatatcca ctgtcttttt ctgcttctaa atacatttct cgcaattcct
     6541 cattggctga caggtctata gcaaatggat cagctttagc tagttcgctt ccatagcatc
     6601 gactgtaaaa tatttctact ccgcgtttct ccgcattatg catcccataa aagtttaatc
     6661 caccgaatgc aaataccttt tggggacggc tgttcaacca aaaattcact agatcaaaat
     6721 gatgggtaga tttatgaaca agaagcccgc cgctattgtg cttatttcga tgccatcttc
     6781 tgaaataatc tgccccgtgt tgcgtattta acagccattc gaagtggata gaaaacacat
     6841 caccaatcac atcactttct atcagttcct ttatcttcgt gtgatacggt gcataacgat
     6901 aattaaaagt gactcgcagg cttcttcccg tctgtttttg gaccgttatt atttcttgag
     6961 ctttttcctc atcgatagtc agaggttttt cgcaaataac atcatatcct tttttcattg
     7021 cacgaatgat ataatcatga tgcgttcgat ctactgacgt aataatgact tcagccggcc
     7081 gtacgtcatc cagtagttgc tcaaattgtg atggcataca tgtctttact tttttctgtc
     7141 catacttgtt cacaagtatc cgatttgcat aatccatcct tgtttgagat acatcacaaa
     7201 aaccgactag ttcacaagtt tgatgatacg tagaggcaat tgcctcataa aaaaaacgcg
     7261 ctctcccacc aagtccgact tgtacatatt ttttcttcat ttaaagtcat tctccttttc
     7321 ttttaagcaa aatactgtga caaatggcag ccctggatac agtaatgcag tgagtaaaaa
     7381 atcatgctgt atacaccaga acagtccata caaaaggcat aaataccaca gaaacccctt
     7441 gacgtttttt gacaacagcc agatattcct tttgatcaaa taaacaagac tatctgcttt
     7501 atcttctttg cagatatcaa ttagcgtact aaaaaaacta tgggatacaa aaagcaacag
     7561 acacaataga aaaggaatca gataccaagc tccaatcttt cccactaata aataccaatc
     7621 aactgcaaaa ataatgacaa acagctgaaa caacgtaatg atgaaacaca tccgtttgat
     7681 ttttcctttc ctccaacata cctttttttc tgccaagtaa tggacagata gataataaga
     7741 aggatacatc atcaaaaaat aaatcagata gacaacacga gcagacagca ctaatcctaa
     7801 agtgaacaaa catcctagaa ctaaagcgaa aagagcatta ctgataagac tggttaaaaa
     7861 aatccgacag acatagtagt tgagcttgta aagaaacatc tcttctattg gctttttcat
     7921 attcagccct tcagaccgct tgtactgatc ccctggacaa gatatttatt gaagaataag
     7981 aaaatcagaa aaaccggtag cagcgacaac gtagacatcg caaacatact cgcgtaagtc
     8041 gtactagatc cagcgtctgc aaataatttg atggcaatcg ataccgtgta agatctcggc
     8101 ttgtttaaat aaatcaatgg tcccatgaag tcgtcccatt tccaataaaa ctggatgatg
     8161 atcgtcgtga tgatcgaagg tttcagcaaa ggaaaaataa ttttggtaaa gacactatac
     8221 ttgctgcatc cgtcaataat cgctgattca tctaactctt tggggatgct gaccatgaat
     8281 tgcatgatct ggtaaatgaa aaatgcttgt ccaaaaaaat gtggtaaaat cagcggcaca
     8341 tacgtcccaa ccaaattcag ccgattgtaa ataataaact gcggaatcaa aatcacttgt
     8401 gcaggaatca tcatcgttac taacatgacg acgaaccaga atctttttcc tttgaaatcg
     8461 attctcgata atgcgtaagc aatacaagtc gagctcagaa ctgtccctat cgtcgaaact
     8521 gcagagatga caaacgaatt cctaaagaaa gtaaaaaatg aaattccagc aaaccccgcc
     8581 catccttttg cgtaattttc ccattgaaaa gtctctggaa tcaaattcag cgaaccactc
     8641 ataatctctt gatccgtttt aaaagaacct gcgatcatcc ataagaccgg ataaatcatg
     8701 actagaccta atagcaaaat aaaaccgtat tgaaaccatt tcgttactgt attattcatt
     8761 tcttcctcct ctcattcaca caacgaccca tttttttgat gatttgaaaa tcagaaaggt
     8821 gacagctccc ataatcaaca gcatgatcca cgaaatagca ctagcatacc ccatagaatt
     8881 aaaagaaaaa gcttggttat acacataaag agcataaaag ttcgtggcat cattcggacc
     8941 accttgggta atgataaatg cctgcgtaaa agtcataaac gccgcgattg tctgcataac
     9001 caagttatac aaaatgatcg gcgaaagaag aggtaacgtg atcttgtaaa acgtctgcca
     9061 tttgttcgct ccatcgatct ctgccgcttc ataatacgag cctgggatct ctttgatacc
     9121 tgcagcaaag atgatcattg ccgaaccgaa ttgccaagca gacatcaaaa caagagggat
     9181 catgaccagt ttttgatcgc taaaccagga aagcgcaggt gcgcctaatc gttccaggaa
     9241 aatattcact aacccgtttt tagaaaacaa ctcccgccaa actagagcga tagaaatact
     9301 gccaccgatc aaactaggca cataatacaa agaacgaaag aaagtcgttc ctttgatatt
     9361 cctcgttaaa agataagcaa tcaatagacc aaagatcagt ttcacaggta cagatgcaaa
     9421 tacatagaga aaagtgactc ggatggagtt caaaaaacgg gcatcactca gcatctttat
     9481 ataattatcc aagccgatcc atgagactgg atttcccaaa cgataatcgg tgaacgaata
     9541 ataaagtgac aagatcatcg gtatcactgt tatcaataga aatcctagga tgaatggaga
     9601 agcaaatacg taaccgacca cattgtcttg atccaagaac cgttttagtt tttctttttt
     9661 catgccttcc tcctgctgct atcctagaat gtttgacgca aactcataaa gactctctgc
     9721 tgcttcttct gctgttactt cttcataaat gactttttca actaaaagat tgtattggtc
     9781 ttgaatttcc ggattcaaag cactatcaaa gacattcact tcgccgttgt ccatatcgga
     9841 aatcatttct aaataatcaa atgtcgcttg caaagttgca tcatctgcac gagcgagctg
     9901 ttctcgaata ttcgacatga ttggtacacc acgttctcct tttaaaattt cattggcttc
     9961 ttcactgttt tgaaagaaat caataaactt agcagcttct tctttatggg ctgagctttt
    10021 tgccatactc agcatttgcg aagaacgtaa agggatacca gattctccat ctgcttttat
    10081 gcgaggtgga ttaatcaact gaatctccat atcttcagga gcccctgaca atatttctgt
    10141 caactggtta cttgatagaa acaccattgc cgcttctcta tccctaaccg cctgaaaaga
    10201 cagatccgtc gtacttgcag ctccaccacc tggttctgga tgggcccctt tgtctaccag
    10261 atttttacgc agctggaaaa aatctgttag atagtctgga ttttcaaatc ccaagctgtt
    10321 agatagatct tcccgattat aaaaattcaa tcctttctca acttgtggta tttgaataat
    10381 acagccagct ataaaatctt cgaatcttga aatagcatag attcctaatt cttcagtgat
    10441 ttgatagctg atttgttcaa attcatccca tgtccaattt gaagctggtt cagggatatt
    10501 taattgttca aatatatttt tattgtaagc aatcgcaaac gaattcaccc cgttagacag
    10561 tccaactaat tgcccgtcgt aagtcgtcgt tgctaaatag gattcatctg cattagaaat
    10621 atcaattatc ccttgctcca catattcatc taatggttca atctgattga tgtattgagg
    10681 aaattgattg ttcagttgga aaatgtccca gatatcatct gatgctgcta gtgtacctaa
    10741 ttttgtgtaa tatccttcat aatcataaaa ttcataatcg atcgtcacat ggggattttt
    10801 ttcttcataa agctcgatta cttttactgt tgcatcgtgt cggacttgcg aaccccacca
    10861 agccatgttc agcgtgattt ttttgtcatc ttcttgccca caagccgtca agaagaataa
    10921 cataacactt aaagctaaga tgattctcat tcttttcttc attctctgca cctctacttc
    10981 cattgttatt tttcaatata ctgtattttt atacagaaca tatttattaa acgatctttt
    11041 tcaaggggaa taaaaaccaa gacttgcctt atcgttctgc ctaggatgaa caactcaata
    11101 ctttcaagaa aaattagata gtgctgattc ttctttcatc gctaaaaaca cttcttgctt
    11161 caatgatggg aggataatta cgcaaagaaa aaaggatata agacaactgc ccaatatcct
    11221 tgacatagct aaataagcat gcttcttcgt gtgcgctctt ccctaaaaaa cagaaaaata
    11281 actagtaatt ctgtatgaaa ttaggtacaa tatacacgtc gatgcacaag cattgactat
    11341 ggcaacttga ttggtcagtt cccgctgact tttcaagccc cgacaattta gctgctacta
    11401 aattgtcggg tgcctttttt agtttttatt cgtttttcat tctatagaaa gcgcttcaat
    11461 aaatcaagac atttttttgt tagaatgcta ataataacaa aaggttttaa ctcttcttat
    11521 gtacattaca ggtaaaaaaa caaaaaaatt tctctttttt ttgaattctg aacaaatagt
    11581 actggagtac aaaagacgag gaagagccac tatctgctct tcctcgtctt ttgattctca
    11641 cctgttattc atgattcttt ttctcagttt cccgatattt ttccatttta cgactaaaaa
    11701 gctctccgct acttggtggc gtatatgtga tggcatttgc tcctgcttga atggtctcag
    11761 caatactttc ttccgttgga ccacctgtcg caatgatagg aagttcagga tattttttac
    11821 gaaaatggcg tactgcttct gcagtttctt tacctgcact gatattgata atcgaaatac
    11881 cagctgctaa cttttcatct atttctgtat atttcgacgt taccgtactg ataactggaa
    11941 tatcaactac tcgacaaact tcttttactg tctctaaagg cgtaggaccg ttcaaaacga
    12001 cccctaacga accttgcgct tctgcgaaca tgctcatgta ggaagaacga gcaccttgtg
    12061 tcaaaccacc acctacaccg gagaaaactg gaatgtcagc tgcctcaata atgcttttag
    12121 taatcgctgg atgaggtgta aaaggataca cagcaattac tgcattcgca tttgtattac
    12181 gtatgatcgc aatatccgta gtaaagatga tcgaacggat caattttcca aatatcttga
    12241 tcccgcttgc ttctaaaatg atttccggta ctcttacgat atcttttctc aattccgtca
    12301 tgatttctgg cgtaaattgt tctgccatca gccacttctc ctttgctatc tccggtcata
    12361 ctgattcaat tgatacgtct cgatgacctg gatgattttt tgcgcataac cggggtctgt
    12421 cgcataacct gcatcttgca atgcttgtgc ggcttcctga tagttggtgg ccgtaatcac
    12481 tttttcatac agcgcaggat tccaatctac tccttggacg aacagttgtg tatggtcatc
    12541 catggactgt tcccatgaat catacacttt gaagtctcct tggatagtta tccattcttc
    12601 gttaacaaat tcctttgttt ccaggcttac tttcttctga tccccataag ccttgatccc
    12661 gaaaagatta ttgtatttgc tagccaaggt actttttccc caatttgatt ctaatatagc
    12721 ttgtcctaaa atgatactag gaagcactcc ataaccatct tgtagttctt gggcatgcgg
    12781 aacaagccgt tcaataaatt gctgatggga ttgcgcttca ttttcttgct gattatcagt
    12841 aaaaggagaa gacagattct tcaaggaaaa aacaaaggct aagagtatca ctgctacacc
    12901 ggcaaccact gctggccaac ggatattctt gcgcttttgc tttttccact tttttttcgc
    12961 catgatcaat tcctactttt catttaattc attcagatat tcttctagcg tcaattggtg
    13021 tttggtgata tactcagcgt tttctttacc gacataacgt aagtgccaag gttcatatgt
    13081 gatcttagtt atatcttcgt gtccttttgg ataacgaacg ataaatccat actgcgcagc
    13141 attatctgcg atccatttag ttccaggctg atctccgtaa cttgcatcca atacagtaga
    13201 aggataactg ttgtaccagt tttgatcgac gacatcgact gccagacctg tgtggtgttc
    13261 gctgtatcct ggctccgtaa tcgtttcttt agtaatcttt gtcgcctcct cttctgatac
    13321 accttgacga ctcatcacat cttggacatt ttgttcgaaa acttgttgtt gggatgatac
    13381 agaccggtaa gctgagacca tcactaatgg aaatccagct gcttcagcag ccttggcaaa
    13441 tgcttcatat tctggagcaa tgcgtttgtc gatcaagtat ccgttactta atgatgccaa
    13501 ttgagtagac tcatcaattt ctttttctaa tttgtgatct ggaccaacca aaactagctt
    13561 ccaatcgtct gttttaacat ctggcaattg tttggacgta cgatcttttg tttctttgtc
    13621 ctttaccttg ctgctggttg tttgactgtt cttttcttta ctagcagttg ttttggtgct
    13681 tgttcgttcg actgcttgat tgccggcttg aacatgcgaa tcatcatcgc taaaaataac
    13741 atagcccatt gctgcgatca taatcacaac tgctccaaat cctaaaaatt tattcacttg
    13801 aaaaatcctt tcgttgttct tcacgttccc cattcgcctc atgaacgtaa gtttcttcca
    13861 gattttcatt cacccaatcg agaagttcga ttttttctcc ttcgatctgt ttttgaacat
    13921 ctgctggcag acgaaaattc aaaatatcat catagcccca ttcatcatat tgaatactta
    13981 ctttcaagtc aaggtatcta ccagcctgct gattattttt caaagggatc agctcgactt
    14041 ttgctgcttt gctgctaagc catttttgcg cgatgcgtgt tttgatttgc ttgtactctg
    14101 caacggcaac gtttcgtttt tcgggaaaaa taatcgtttg acgcaaaatc ttttgcgtta
    14161 gcatccattc ataatcagtg aaaagatttt ttactttctg catcgcttca gatggcagat
    14221 ttttctcgcc atctttccat ttttgccatt gttctggact gatcagcaag tattcatcta
    14281 aaaaattttt ttcagtttca aactgttcta ataatacact cataataaga tcaatatata
    14341 cttgatgcac gtttatcccc tccttttaac tattctactt aagttttgat caaaaagcac
    14401 ctcttaaatc gctgctttca ttttttttaa tgtttcaagt cctctcctct ttattcttcg
    14461 tgatttttgt tataataata gacagacaag aaactcaatc caactatgaa ggagcgtttt
    14521 cagatgaaaa aatatcaatg gggaatcgtt ggtcttggta ccatcgcaca tgagtttgcc
    14581 gaaagcttca atcaagagac gagcgaacta acagcagttg cttccagaac ttcagaaaaa
    14641 gcggaaaact tcgctcaccg ttacaatatt ccaaaagctt acggaagcta tcaagaaatg
    14701 ttagacgatg ctgaaatcga catcgtgtac attgctgtac caaatagaca gcatatcgac
    14761 catatcttgg cagcattgga agcaggcaaa cacgttctat gtgaaaaagc catcacgatg
    14821 aataaaaaag aactggctga cgctatgcgc ttagcagaag aaaagaattt gatcttagca
    14881 gaagcgatga caatcttcaa catgccactt tatcagcagt tacgctcaat aatggataca
    14941 ggaaaactgg gggctttaaa aatgatccaa gcacctttcg gcagttacaa agaaccagac
    15001 cctaaaaacc ggttcttcaa tccagaatta gctggaggag ctctgttgga tatcggtacg
    15061 tatgctgtat ctttcgctcg tttctttttg agctctcaac ctgaagtagt tgcctctaca
    15121 atggttcctt ttgaaacagg cgtggacgaa caatccgtca ctatcttgcg taacaaagaa
    15181 aatgaacttg ctgctgtcag cttgactttt caggcaaaaa tgccaaaagt cggtatcgtt
    15241 gcctttgaga acggttatgt gacgattgct gattacccgc gtgctgatcg tgcggagatc
    15301 ttatttacag atggtacaaa ggaattcatc gaaagcggga atacatctga agcaatgaat
    15361 tacgaaataa agaatatggt acacatgatt gaaggtgact tgcccaatcg ctctctcttc
    15421 ttgacaaaag atgtcatcga aatcttggat caaatgcaag ttctatggaa aaatatgtaa
    15481 ttttaaaagg ttgtgaaaaa gctgttctgc atcaattaat aagaacagcc aaacaaaaat
    15541 agctcctcct atttttcgtt tggctggact tattaccgca gaggaaagct tttgaacacc
    15601 gtttaagaaa gaggttgtga gcctaccgtt cagctccgag tcacaaggag caatttcaaa
    15661 aaacagcttc tcatgtttgt tgaatattgt gacttgtgac cgaggagttg acaggagaac
    15721 accgtttaaa gaagaggttg tgaaatcagc tttttgacct gaatattagc tgaattcaca
    15781 acctctattt ttggattttt tgttgggaga attctgcctt gatttcaata aaacttttaa
    15841 aagactgcaa aaactggaac aattctgctc gatcctcgaa ttcagggcga ctagtcggca
    15901 gaggcatttg ccgatacaat acataaactt cttcaatacg tgttaaaatt tgtctgccat
    15961 catttgactc tgaaaatgtt tctgcagtat aaatcaataa accatagata tgttttccat
    16021 aataagctgg ttgctggatt ctctctagat tttccagcat gtctttaatc acattggcct
    16081 gtgctcttct catagagaag taagtttcgt agtatagcgg ctgtctgagc cagtagtttt
    16141 cttgatgttc tctggcatct tcttggcttt cgcgaatgaa tgtcagtaaa tgttcgcatt
    16201 tgatctgcac atcttcttta ttttctgata aaagaaattc tgccatttgt ctcaaaatat
    16261 ttcgaaattc tttttcgatc tgtatttggt tttccttcag ctgcttcgta cgatctggca
    16321 tgtaaacatt tgcaagcaaa gcaaatacta atccaatcgc cattaatccc gcttcattgc
    16381 cgatcagctg ccaagacatg ttttcctcca ctaaataatg agtgaccaaa accgaattta
    16441 ctactatccc ttcggttaat ttgaagcgag aggcaagcgg gatgaacagg aataaaaaaa
    16501 gactgaagcc aaaaatagta tacccaacag caaaaaaact gaacagtgct gttgccgtag
    16561 ctaatataaa ggctgcgatt cgattcatgc ccgtacgcaa tgtagagcgc ttggtatttc
    16621 ctacacttaa taatgcaatg atccctgcag ctggccaata caacagcgat aagctacccg
    16681 ctgtgaccat cgaaaagaag acactgatca ctgttttgac tgttcgcatt cctatacgca
    16741 tttacttccc ccttttttat tttaattata cttgaataat gaaaaactag ctatcagaca
    16801 aaagtttagg tatgatagta aaaagaccaa aaaggagaat ctctatgcaa tttggccgtt
    16861 ttcgactagg tatgcgtacg atcaaaagtg ccttagctgt ttttttatgt atcttgtttt
    16921 ttcatttgac tcaacgagga atcccgatga ttgctgcatt atctgctgtt ttttctttgc
    16981 gtcaggactt gactacaacc gtttcttttg gaaagtctag gatcatcggc aatacattag
    17041 gtggtacatt agctatcatt tatttttttg tcaaaagcta ttttcacaat gattttttgg
    17101 tagaattgat cttgcttcct attctagtta ttattgtgat cgtcgtgtca gatggtatta
    17161 acaataattc cggtatcatt tccgcaatag ctacgttgtt gctgattgcg ttgagcatcc
    17221 ctcaaggcga atccgctttg tatgctatcc aacgtgtact agatactttt atagggacat
    17281 tcattgcaat cggtatcaat ttctttcttc gtcctcctga aacagaaaag aaggaagaac
    17341 tatcagaaga tttggtcgaa ctccaaaaga aagaacgatt gctaagaaca aatttacaag
    17401 agatagaagc taagatccaa aaacaaaaga aaaatagcga ggactaattt tcttttgcaa
    17461 taaaataaaa aaatggagag aagaacttta aaaagtcttc ctccattttt gattgaacgg
    17521 tgaagtgtac tactttattc ttccttcatc gagttttgtt ctgcctcttt tggcgcttca
    17581 tacatattta cttggctagt agtcccacgt gtcgacaaat tctctacgat ttccttcaag
    17641 tcaagtcctg ttgtcgcttt tagtgtttct tgagtagaag aaagcaaatt tgtggcataa
    17701 tttgtcactc ggttcgctcc actggtttct cctgaactgc ttcctgtatc gacaacagag
    17761 attttttcga tatttcctag cggctgtgct gcttcgcgca tcaattgcgg tagcatatcg
    17821 ataaccatac tcaagacagc tgcttcgcca tattccttga aggcattggc gatcttttgt
    17881 tttgcttcgg cttctgcttt tccttttgct agtgctgctt cggcttctgc ttgtccagct
    17941 aaacgtgtct tattggcttc cgcttctgcc agtgcttcca cacggaatcg ttctgcttcg
    18001 gcttcagtca cttctttgac tttttgagct tgtgcttctt gttctttagc gtaccgatcg
    18061 gcatctgctt ttttcttcac ttccgaatca tattgctttt cacgacgtgt gatttctttt
    18121 tcttccagtt cgatttgttt ttgacgttct acgattttaa tctgcatttc ctgttcgatt
    18181 acttgttgtt gtgctcgggc actttctaaa ttgtaggctt ggtctgcttt tgctttagca
    18241 acatcttgtt cttgtttgta ggttgctaat ttcagttctt tttcttttaa tgattctgcg
    18301 atctctgttt gtcgctgtaa ttcagctgct tgtgattctt tttctgcttg agcttttttg
    18361 atacgcgttt cttttagagc ttccgcttct gctatatctg catctctttt gacttgggcg
    18421 attcttggtt tccctaaaga gtcaagataa ccgtttttgt ctctgacctc tttgatagtg
    18481 aaagaaacga tgatcaaccc catttttgca aggtctacag aagcgacttc ttgtacgctt
    18541 tgactgaact tatcgcgatt ttgatagatt tcttctacag tcatagaacc aagaatcgag
    18601 cgcaagtgtc cttctaatac ttctcttgct tcattttcca gttcttctct tgtcttgcct
    18661 aaaaactgtt ctgctgctgt agcaatctct tctacagatg aaccgatttt gatgatcgaa
    18721 gtaccatcac acatcaccgg tacgccttgt tccgtataca cttcaggggt ggagacgtcc
    18781 aatttactcg ataatagact gatccgattc gaacgttgaa aaactggcaa gacgaatgcg
    18841 ccacctccac gcacaatttt gattttgtta ttgctttcat caatgtgcac atttttgctt
    18901 cctaaataac ttccactgat gatcaacgct tcatccggtt ttgctgtctg gtacttcgct
    18961 acaaagacga tcagcaacat cagtaaaatc aaagctgcga ttgcgatcgc gataaaaaca
    19021 actggtattt ccatttaaca cacgctcctt taagctttta tcatgctttc ataaggcatg
    19081 acataacaga ttctttcctt gatctcaatg atcaatactt tcgttccccg ctcagctgtc
    19141 tgttcttggg ggctgtaaaa acaagctgga cgactaatgg aaccggtaac acttttcaac
    19201 tgaatttctc ccatacctcg tacagggatc ggtgtgatca caacagctac ttgtccttcc
    19261 agagtttttt cagaagacga catcgttgat tcggcatgtt tcatcggcat caatatatag
    19321 aaattaagca aaaataccaa cactatcgat atgcctccac tagcaataaa taaaaataaa
    19381 gatgattgat ctgttaaacg ctcacctaga aaaccaaata agcttgtgaa agttaaccaa
    19441 gggataataa gcatcggatc aagcgggcca tcaaaatcga atacatctcc aaaaataatc
    19501 aataggactg ccaatcctgc acaaatcagc aatgtatacc aataaatcgt ctcaagtgcg
    19561 attcccccta tcatatacac gcctccttcc atttctacgt ctatcatacc aatgtttgaa
    19621 aattctgtat agcttctttt accgactcag tggtgttttt tataggaagg ttgtgtttat
    19681 cgttgacaat ttgttaaatt tttgttttca tgttaaaatt ttcacaataa attctttcga
    19741 ggtagcctat gtttcctttt tttaagaaaa aaccattaaa gctccagaac acgatcatcc
    19801 ttgtcgtttg tttctccaca ttcgtttctt tgattgtttc tgccatattg atcaggaatt
    19861 ttattctttc gatgaaccga tctagtgtgt cagataaaat ctctagcatt gctaaaatcg
    19921 ttgccaatga taaactcatc caaaaaaagc tgcagactgg tgacccgaca catgcgattc
    19981 aaggaataac aaatgaaatc atgattgcat cgaatttaga ttttatcgtt gtcatggacc
    20041 ctgaaggcat tcgatattct cacgcagacc caagttacat cggtcttcct ttctcaagtc
    20101 atgaagatgt gaaaaaagtg attggaggac aagaattttt ctctgaaaaa gaaggaatgt
    20161 tagggttcgg cttccgctat ttcgtcccta tttattatga tcaagagatt attggtgcag
    20221 tttgtgttgg cttaacaaac aacacgattg acaatcaagt caaagatgga caaaaaatga
    20281 ttgttttttc attgattatt ggacttagcg taggcatcat aggcgccgtt tttcttgccc
    20341 aaaaaatgaa aaaagtactg cttggacttg aaccacaaga aatagcagaa cagttaagtg
    20401 aaaaaacgat tatcgaaaat gaagtagatg aaggaatctt agccatctct tctgataaaa
    20461 ctatcctctt gatcaataca cctgcaaaaa acattttcta ccaaacagag cagctattgg
    20521 atttcgaagt aggaaaaaaa tcagtgataa tctatatgac gttttgttta aagagacgat
    20581 tgagcatcaa acgaccgttc ggactcaatc catgtattta aatgggatag aaactatttc
    20641 tagcgttaca cctcttttta gcaatgaccg ttttttagga gcagtcgcaa ctttacgaaa
    20701 tcgatcagaa gtaatgaaat taatggagga gcttgggggg acgaaacaat atataaactc
    20761 catgagagct caaacccatg aattcatgaa taaacttcat gtcatttctg gtttgattga
    20821 tttgaagaag tatgaagaag tcacccattt tatccagcag ctaaatcaaa attataaaaa
    20881 agagattggc caagtctcaa gtctgatcaa agtacctgca attgccggtt ttttattagg
    20941 caaaataaac gaagccaaag aacaatctgt tgaaatgctg ttagaaagcg attcttttat
    21001 ccctaaaatg gagatggatg actccatcca ttcacttttg caagtgttag ggaacttact
    21061 tgataatgca aaagaagccg tgctacaaaa acatgaaaga aacggacagg tccggattca
    21121 tttaaactat gacacagaag ctgaagtatt catcgtgacc gtctgcgatg acggtgtcgg
    21181 gatatcagaa gaaatctcag aaaaaatatt tacgctaggt ttttcgacga agggaatcaa
    21241 tcgtgggttt ggattgaatc tcatccattc aatcgtcaaa cgccacggtg gatttttaga
    21301 attttccaaa caaacaaatg gcggtacaat cgtctatcta gaattaccaa aaaaggaaga
    21361 tcaaaatgat gacaaatgtc ctgataatcg aagatgatcc aatggttgca gaattgaatc
    21421 gtacttttgt ggaaagcatc cctaatatga acgtaatcga aagtttcaga aatgtagaag
    21481 aaggaaaaaa atatttcaga caaaaaaacg cagatctgct gctgcttgat gtatacatgc
    21541 ctggcgaaag tggtatccat tttttatctt ggcttagaca gcaaggaaat gatatcccag
    21601 tgattttgat cactgctgca gatgatatcg aaaccataaa gaatgtgatc cgctacggtg
    21661 ccttagatta tttgatcaaa ccgttcactt tgcaacgttt tcagctggcg atcgaaaagt
    21721 atcttgcttt ctctcagaca atccaaacga ctgaaaaagc cgatcagaaa ttgatcgatc
    21781 aagtattcaa ccacaccact cccattcacg aaataccatc attcatcgat ttgcctaaag
    21841 gtctgtcaaa actgacttta aaaaaaataa gtgcatttat tctaaaagaa gatactattt
    21901 tttcaacaaa agaactggct caaaaaagct cactgtcacg tatatccgtc aagaaatatt
    21961 tgaatttttt agtagaacaa gatatattga acgaaagaat caaatatttg gaaatcggcc
    22021 ggccgctaac ctgttatgaa ttgacaaaga atgggcggaa aaagctcaca gattactgtt
    22081 gagtcatcga attatcccat acaaaatagt ccaagtatac agaagtaaca actgtttact
    22141 tggactattt ttaaacattt tattgacgaa tatccatgtt ttcttatctt aattgacggg
    22201 ctttaggaag agatacctca tttttcgttt ttccagtttc tattatttca cgaaagttct
    22261 cgaaacttgt cgaaatcata tttgataatg cctcatctgt atttgaacct acatgcgggg
    22321 ttacaagaac tctagggtac atttctacta attgctggat agcagggtct ggaatcgtct
    22381 cccatggctt gaaggatcgg aaaaaaacct ctgtttcatt tgcaaaaaca tcggttgcaa
    22441 aaccagctaa atgattgctt ttcaaagctc ttaaaatagc ttcattatct tgaagctctc
    22501 cacgggctgt attaatcaag atgctacctt ttttcatttg agctaaaaat ccttcattga
    22561 tcatttgatc gttttcacct gggatatgag gtacatgaat actgacaata tcgcttttgg
    22621 ctaataactc ttcaagcgag tggaacgtca atactttttt agcagcttct gtctgatgaa
    22681 tatcgtagcc gatcactttg gctcctagtc ccttaaacaa aacagcttcc gtcaacccta
    22741 tcctgcctgt tcctatgatc cctactgtgc aatttctgat ttcacgacta aacatttgct
    22801 catccactcg aaaatctttg aaagatgttc ggatcgtcgt atacgcagta tttctcaaaa
    22861 gcatcatggc aaaagtcaaa gataactctg ctatcgcatt tggcgaatac gatggtacac
    22921 gggctactgt cataccaaaa tcagctgccg cttgtagatc gatatgattg actcccacag
    22981 tccgtgtaaa aacataacga atcccatatt cctgcatttt tgcaagattg gtctcatctg
    23041 ctatacaatt tcccctcaac aatacagcat catggtcctt tgctgacacg atattgtcat
    23101 gtgtcaacag ttcttcataa agactgagat cataatggta aatattcaat ttttcaaaaa
    23161 aatctatctc gttcggacga acaccgtaac atgcgattct catactttct tcctccattt
    23221 cttatgaaaa taaacctatg ccatgaatca tttgtaatac aacgatccat acaggtgtca
    23281 gtaccacagc aaaaatagtt gacagtaagg atgcattgga ggctaaaagt gcctccctat
    23341 caaaactgat agcataggct gctgccactg ttgcagctgg tgtagccatc atgataacga
    23401 ttgttgccag tgcttcaaaa ttgactggta gaatattcgt tgccgtcaaa actgctagaa
    23461 tcacgatatt caatgcaggt acaaatacca ctttgactag actgtaatac catgaagttt
    23521 tttcagatgc agcttttttg aagcttactt cacctaatgt tgcgccgatt gctagccacg
    23581 ccaatggtga acttaaacca gctaaataat ccatcggctg aaatagccac ggtgcagtct
    23641 ggtcaattcg caaaaacgct acttggctta tctctccttc tgcacctgta actgacactt
    23701 gtggcagata cccttggaac agccaaatga aaaggccggc aaaagtagcg ataatgattg
    23761 gattgacgaa catcgctttg atatttttag cttccatctt caagccactc atttttatgt
    23821 atccataaga atacaaaaag attcggtagc caatattgaa aattgaggaa tacatcacgc
    23881 cgacaggacc ataaatcgca ctgacgatag gcgttccgaa aaaggtcgta gaaccaaaga
    23941 tcgtcaggac acgcaacgtt gtttgcttat cccctttata gcttaagaaa
//
LOCUS       DAXWOO010000028        23812 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.028, whole genome shotgun sequence.
ACCESSION   DAXWOO010000028 DAXWOO010000000
VERSION     DAXWOO010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.028"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            72..758
                     /locus_tag="ACGE70_000879"
     CDS             72..758
                     /locus_tag="ACGE70_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFF8692532.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            760..1704
                     /locus_tag="ACGE70_000880"
     CDS             760..1704
                     /locus_tag="ACGE70_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFF8692533.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            1903..2547
                     /gene="pcp"
                     /locus_tag="ACGE70_000881"
     CDS             1903..2547
                     /gene="pcp"
                     /locus_tag="ACGE70_000881"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFF8692534.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            2626..3903
                     /locus_tag="ACGE70_000882"
     CDS             2626..3903
                     /locus_tag="ACGE70_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692535.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(4087..4836)
                     /gene="pnuC"
                     /locus_tag="ACGE70_000883"
     CDS             complement(4087..4836)
                     /gene="pnuC"
                     /locus_tag="ACGE70_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFF8692536.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            5166..5546
                     /locus_tag="ACGE70_000884"
     CDS             5166..5546
                     /locus_tag="ACGE70_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFF8692537.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            5642..6151
                     /locus_tag="ACGE70_000885"
     CDS             5642..6151
                     /locus_tag="ACGE70_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692538.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            6228..6407
                     /locus_tag="ACGE70_000886"
     CDS             6228..6407
                     /locus_tag="ACGE70_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692539.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            6462..7025
                     /locus_tag="ACGE70_000887"
     CDS             6462..7025
                     /locus_tag="ACGE70_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8692540.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(7072..7587)
                     /locus_tag="ACGE70_000888"
     CDS             complement(7072..7587)
                     /locus_tag="ACGE70_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFF8692541.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(7613..8482)
                     /locus_tag="ACGE70_000889"
     CDS             complement(7613..8482)
                     /locus_tag="ACGE70_000889"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFF8692542.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            8608..9288
                     /locus_tag="ACGE70_000890"
     CDS             8608..9288
                     /locus_tag="ACGE70_000890"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFF8692543.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            9441..10421
                     /gene="pta"
                     /locus_tag="ACGE70_000891"
     CDS             9441..10421
                     /gene="pta"
                     /locus_tag="ACGE70_000891"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFF8692544.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            10544..11017
                     /gene="tsaE"
                     /locus_tag="ACGE70_000892"
     CDS             10544..11017
                     /gene="tsaE"
                     /locus_tag="ACGE70_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFF8692545.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            11010..11534
                     /locus_tag="ACGE70_000893"
     CDS             11010..11534
                     /locus_tag="ACGE70_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFF8692546.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(12024..12557)
                     /locus_tag="ACGE70_000894"
     CDS             complement(12024..12557)
                     /locus_tag="ACGE70_000894"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFF8692547.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(13421..14173)
                     /locus_tag="ACGE70_000895"
     CDS             complement(13421..14173)
                     /locus_tag="ACGE70_000895"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFF8692548.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            14286..15206
                     /locus_tag="ACGE70_000896"
     CDS             14286..15206
                     /locus_tag="ACGE70_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFF8692549.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            15279..16214
                     /gene="murB"
                     /locus_tag="ACGE70_000897"
     CDS             15279..16214
                     /gene="murB"
                     /locus_tag="ACGE70_000897"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFF8692550.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            16512..17990
                     /locus_tag="ACGE70_000898"
     CDS             16512..17990
                     /locus_tag="ACGE70_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8692551.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            18267..20327
                     /locus_tag="ACGE70_000899"
     CDS             18267..20327
                     /locus_tag="ACGE70_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFF8692552.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            20410..20979
                     /locus_tag="ACGE70_000900"
     CDS             20410..20979
                     /locus_tag="ACGE70_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8692553.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(21154..21960)
                     /locus_tag="ACGE70_000901"
     CDS             complement(21154..21960)
                     /locus_tag="ACGE70_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692554.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            22098..23612
                     /locus_tag="ACGE70_000902"
     CDS             22098..23612
                     /locus_tag="ACGE70_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFF8692555.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         8042 a         4116 c         4839 g         6815 t
ORIGIN      
        1 aaaacaacta tattttttat gtgttttgct ttataattaa aaaaatattg tcattttatg
       61 gaggaaaata tatggaaaat tatcttagcc ttttaggcgt cttgattgtt attctcgggt
      121 ttgcatttaa attagactcg atattgatcg taatggttgc tttagttgtt acagctttga
      181 ctggcggtct tggcattcaa ggaatgttag aacttttagg tacaagtttt gtaaacaatc
      241 gcgggatggc gatttttatc atcattatgt tggcaacagg aacgcttgaa agaaatggtc
      301 tgaaagaatc agcggcttcc ttgatcaagc gattcaagaa agtatctgct ggtatgattg
      361 tagatattta tgggatcttt cgaatgatct ttgcggcttt taacgtgagt tttggtggag
      421 ttgctggatt tgttcgtcca atcattttac cgatggcatt ggggaccatc gaatcaaaag
      481 ggttagaagt ccatcctaag catgaagaag aactaaaagg tatggcttca gctatggaaa
      541 atgtctgctg gttctttggc caagtactat tcatcggagg agcaggggcg ttgctagtcc
      601 aatcaacact gaaagatcta ggatatgacg taacattggg acagcttgct ttagtggaag
      661 ttccagtggc tatcacggct ttagttgtgg caagtgtcta cttctatctt aaagaaaaga
      721 aattagcgaa gaaatattat ccaggaaagg ggcaataaga tggacttttt tacaaatcct
      781 gatattttat taggcgataa attactggag atcctttata tcgtgatggg actcgtattg
      841 atttacactg gagtaagaaa tttaacagat aaaaccaatc ctcatcgtta tgggtcagcc
      901 tttttctgga cggtcttagg ggtagttatt gcaggaggaa gatggcttcc ggcaatcgtg
      961 aacggtggct tgatctttgc gatgaccata ccagcaattg ctaagaaagt cagcaaagga
     1021 gaaagccggc ttccatcaaa agaatatatg gaaaaaatgt cagacaaact aggaatgaaa
     1081 atttttattc cagctttgag tatcggtgtc tttgcgatct tgtttgctct atttaccaat
     1141 ttaggtgctt tagtcggcgt gggcgtcggt gtttttgcag ccatgctaat cttgatgttc
     1201 ttttctcgag ataatcgacc aagtacattt ttagatgatg cagcagatat gctgggcaca
     1261 gtagggccgt taagcatgtt gccaatgctt ttagcttctc taggtgcggt atttacaagc
     1321 gctggagttg ggacagtgat ttccaatgca gtaggtacga tcgttccaga aggaaatgtg
     1381 aacatcggca tcatcattta cgctcttggc atggtgatat ttacggtaat catggggaat
     1441 gcatttgcgg ccatcaccgt gatgacggtt gggatcggtg caccattcgt atttagtcat
     1501 ggagcgaatc cagcgttaat aggaatggtt gcattgactt gcggtttttg tgggaccctg
     1561 ctgacaccga tggctgctaa cttcaatatc gtgcctgtag cgatgctgga gatgaaagac
     1621 aaatacggag tgatcaaaaa ccagttgttt attgcgttat ttatgctagt tttccaaatt
     1681 gcttatatga ttctatttaa ataatagaag agaggggttg tgacagaata attgtcgcaa
     1741 cctcttctaa cattgtagtg gaacactatt tttttataag aatatattaa gtttgatctg
     1801 ttatgttatt taaactattg tttcctttat ttacgctgtt caaggaaaat ggtatgataa
     1861 aagataacca tatgttaaat caatcggacg gaggaacaac agatgaaagt actagtaaca
     1921 ggttttgatc catttggtgg ggacaaagtg aatccagcct atgaagcagt aaaaaaaatg
     1981 ccagatgaaa tcagcggtgc tgaaatcatc aaagtggaag taccgaccgt attcgaaaaa
     2041 agcagtcaag tagtcaaaga agccatccaa cagcaccaac cagatgtcgt gatctgtgtc
     2101 ggacaagcag gtgggcggtc agcagtctcg ttcgaacgtg tcgcaatcaa tctagcagaa
     2161 gcaagaatcc ctgataatga aggaaatcag ccattcgata cggctttaga agaaaacggt
     2221 ccagccgctt actttacctc attgccaatc aaagcaatga caaaaaatgt acaagatcat
     2281 ggtctaccag cgtacatctc ttatacagca ggaacatttg tctgcaatga tatcatgtat
     2341 cgtttgcttc atttgatcga aacacaattc ccggcaattc gaggtgggtt catccacgtg
     2401 ccattctcac ccgatcaagt gatcgatcgt ccagtgggta cgccttctat gtctttagaa
     2461 gacattgctt cttctttgac ttatgcagtt gaagcagcag tagaaaataa agaagatatt
     2521 tccggaaatg ccggaacaac tcactaattt agcaagaaac actggctggt tgctaacgtt
     2581 atgttagtag ccagcttttt gtaattagaa aggaaagaaa cagccgtgga agaatggcaa
     2641 tccgtctttg aagaatggtt tccaaaagaa atcagcaaaa gctatcccat caaaatttct
     2701 aaacaataca caagcagtca acgttgggag atctatgcaa aattaacgaa aaaacagcgg
     2761 gaactagtcg ataagcaccg ccgatatcta atcagctctc gttttatgga agaacattat
     2821 ttagctgcaa cagattgggt gttttctgac ttcaagatca atccgttttt tcgaacaaaa
     2881 cgaagccagc aaaaattgta ttgtgagtgt ggtagagaat taaaagtgca atacatcgtt
     2941 aaatcgccaa aaaccggaaa gatcctaaaa ctaggcatca accactttgc cgatcacctg
     3001 catgtctctc cgacagtggc agcttcgatc catcagggaa tgacaaaagt tgatctggcc
     3061 ttagacgaat tattatggct taagcagaag aatatcgatt ttcctgaagg actatggcaa
     3121 aaatactgtt ttgtcttgta ccaaaatcgt cggatgaaac agccatattt accggatata
     3181 aaattagctc agcggctcgc agaatttcgc caagtagaaa tgccgattta catagcggat
     3241 tatcaagcat tggaaaatga aatcaagaaa ataagtgaac acatcaatgg gcagtcaaaa
     3301 aagcgacaga taaaaaaaga actatttgac gactttgcag aagaattagt aaaggatgta
     3361 gaagaattct tgatcaacta tcgcgcattt ttacgtaaag attggcaatc catcgtatat
     3421 gaagaagtgc cagtacatcc taatgcttat tttgaaacat ttatttccgt gttgagaaag
     3481 acaaaacgtc aacgaacacc agaagtcact gctcagatgg aatattttgc gaaaaaccaa
     3541 cgattcatcc aaccaaaaat ctatctattt atttggaagc aatattgtcg ctatggtttt
     3601 acagaagggt tcttcgacag cattcctcgg atagtcagaa atggtttttt aaaagtattg
     3661 cgtaaagaac gagaagctat acaatctgct gataagaaag accgtacagt ctcaaaagaa
     3721 aaatggcaac tagtagtgaa agatatccaa tctggaaatg tgcaagagac aatcgacaaa
     3781 tggaaaggaa aacattatcg ttttactgaa gctcagaaac aagcgttgga atattatcag
     3841 aagttagaag aatcactgcg tttcaatgat gaggcaagaa agtatctgaa ggaattacta
     3901 taaaaaatag aggctgggac agaagcgttt aactccaaga aataagaaga aattcacgaa
     3961 aattgctttt caaatttttg tgaatttcag cttatttccg aaggagttgc ttctgattcc
     4021 gccgtttata cgtgtttaga gcgtgagaca aaagtgttct ttacttttgt cccacgctca
     4081 gtttttttat gcgtgttgtt gtgcgttgta gtattcttct tccatttttt gcatacgttc
     4141 acgttctttt tggttatacc aaggtgaaac ggtaaatgca aggacatcat tcagcaagta
     4201 aacagagctg ttcacgaaca tcgccaaact tgcagatcca tgcgtaaatg aaatatacca
     4261 aaggaccatt tgagcaagcc ctgaagctaa ccaccagtaa tattggttgt tgtaacgcaa
     4321 gaaactgatg ataccagcag tcaagctaat agaaaaagca atggcatcta cccacggccg
     4381 aggatcatct gtataacgac caatcaggaa tcctgaaaca agatagacaa ggaaagtact
     4441 gatgattgcg ataaaccagc tttttccagt gaattttcgg atttttgaag ccatattatg
     4501 attccattct ttacttaaca atacggggat atccaatgtg atcatataag caatctgttc
     4561 accgatactt aaatagttct ttgctgaaaa cccaacgata ataaaacaaa tcgcagacaa
     4621 aatacctaaa ataccgttga ctgattttgc cgcattgatg gataaaatac acaatacacc
     4681 aagcgtggta ccgataaatg taataacgga cagccaagtg atgggactat tgacaagagt
     4741 catgacctgg cagccaagac tgaaaaagaa aagataataa ttttgttgcg gccagccttt
     4801 tagctgatga gctaaaaact gaaaatatga cttcatgtat gaggactcct taagacacta
     4861 aatgtagtgt gttctgtatt tactaacaca atctattgta tgcttttctt gaaaaaaaac
     4921 tagtctttca attataaata atttgtgtta tgataatttt ctgtttgttt tcttgaaaat
     4981 ataatttctt actttcattt attatattgg gaacaggaag agtgtcttgg aggacctact
     5041 ttctgttttt tttgcctttt ttcttataaa aaggaaaaac agctattttt ttcaatctga
     5101 cacatttaca atggaactgg agattaatat agatccgagc ttaaacgaag aataggagtg
     5161 ctcatatgaa ccttacaacg atccatcata tagccatcat tgtttcagat tatcagaaat
     5221 cacgtgattt ttatgttaat aaattaggat ttgaagtaat cagagaaaat taccgcaaag
     5281 aacgagatga ctataaatta gatctgaaac taggaagtgc tgaattggag atttttggaa
     5341 ttgctaatgc gccacaacga ccaaactatc cagaagcttg tggcttgcgt catcttgctt
     5401 tcaaagtaga cgatattgaa gaaacaattg cagaattgca gtccttaggg atcgaatcag
     5461 aaccgattag agaagatacc tttacaggaa aaaaaatgac gtttttcttt gatccagacg
     5521 gattgccatt agaactgcac gaataaagat aacaaaattg tcactttttt ttcgtttcat
     5581 tcgatttttc attcgttcat ttcttgatat tctttaaaaa aaagaaacag gagaatgaag
     5641 aatgaaaaaa atgatgtttg gtgttgctgc cctcatcgta atagggatta ccggtatgac
     5701 gggactgctc aatgccgctt ttcacatgga tgatattagt gaccaggaat atcggatctc
     5761 attgaaaaaa gcagcagcaa tcgtaaaaaa cgaaacaaaa gaacataaca tatctgctat
     5821 tcattttgca gttgaaaaag atttggagaa tcctacgaat gcaacatatg aataccagtt
     5881 tttttgtgaa ggacaagtca tagcagtcaa tccatccact gggaaaacaa ccgtacgaaa
     5941 tgacacggaa aaggccgcag aacatctttt tgatatagaa aaaacggaag agttcaaaca
     6001 accgcaagca gcgatgaagg aagcagtcag ctactacggg aaaaaagcaa aagtgaaaga
     6061 gtggacactt ctagtcaaag atgaacgtcc ttattataaa gtccgattaa tagatggaga
     6121 acaggaccgt caggtatgga tatctgcata gaagaaggat attgcttctc ttttttacaa
     6181 tataagagta agctaagaga gtaaagatga aaggaagttg agataacatg gaaaaaatga
     6241 aatgccctaa ttgcggaaaa aaatttgcat acgaagaagt caataatgta gtcgaacata
     6301 atgataaaga aatgccagtc gtctgccctt attgccgaac tgaagcggca agaatcgtta
     6361 ctcatggtta tttcattact gaaaagattg aagatttttt aaaataacga ttttgcgatt
     6421 tctttttctg catgttagaa tggagaaaag gagaggaaac tatgattcgt tttgcgagaa
     6481 aagaagatgc tccagaaatc gccccattga tcttagtgat tctaaaagat atggaactgc
     6541 catttttgaa gcagacaggg gaacaaaaga ctttagagat tttagaagaa gcaatcactg
     6601 atccaaatta tcgttacagt tattcacgag gtatcgtaga agaaatagac ggtaaagttg
     6661 caggagttgc ttacggttat acagatgaag aagaatcaat catcgatcag ccactggtac
     6721 ctatcttaga gaaatatcag ctagatgcgt ctacacgttt gttcattgat aaagaaacgt
     6781 ttcccaacga atggtatttg gactctatca gtgtatcaga agattttaga ggacaaggca
     6841 tcggttcgcg attattagaa gcattaccta aattggcgaa gaaagctaat cgctccgtca
     6901 tcggtctaag tgtggatgaa aaaaatccaa aggctaaaaa attatacgaa cgccatgact
     6961 tcaaagttgt cggtcagcgg atgatcagtg gtcatcttta tgatcatatg caaaagaata
     7021 tctaaaaaga tgtacctgaa atgcgtattt ccgcatttca ggtacatctt tttaacttaa
     7081 gttaagtcgg taagaaacag gcttatcgcg acttttgtct ttacggtaga gccagtaatc
     7141 taaaaccgca gcaatacaaa tacaaactgg agcatcttca tcatttgaga catccaatac
     7201 atagtaatct cctgaaaaaa gtgttgcttt atccattttc atgatttgct gattgaaatg
     7261 atggatttga tattgatgat tatagatatc accttggact gtccagtgaa gtctacgaat
     7321 ataataaaag tcaccgggcc aattgaatat tttctgcatg gttcccacct tttcgaactc
     7381 tttatacagc tcaaaacgcg taccgaatgt caggcttatc tgcttgatgt gagcaacgag
     7441 atcgccattc atggcataaa gagacaatgc atcgcccttt gttccccagc gaccaaccat
     7501 caaaaacagg gaatgtcctt tttcgttttt gacaattgtc cgagttgcac gtccaagctg
     7561 cttttcctga atgaaaaatt ctgacatcta tatccctctt ttctagataa aactacaagt
     7621 cttctgcaat cgcgcgcaat atggcttttc ctacgccaga ttcaaggttt gtgtccgttg
     7681 tatatttggc ataatctttt acttcgattt ctgcatttcc cattgcgaaa ctgactcctg
     7741 ctacttgcag catgctgaca tcattgaagt tatcgccaat cgtcatgact tcttctaaag
     7801 aaatcccacg ctcgtgagcg acatgggcaa cagcaattcc tttttgagcg tttttatggt
     7861 tcacttcgat attgttttga ccagaggaag taacggctag atcgcctaaa agagaaatct
     7921 gctttccaac tggacctaat acagctggcc cttctgtatg aaaacaaata atttttaaaa
     7981 cttccagatt cttttcttcc aaaatagcac gaatactgtc cacgtatgtg atgtgcaata
     8041 actccagctt agctgaggcc atggcaatcg ccattttata cgtaaggtga ggtaaatggg
     8101 tagccaccat ggaagcaaaa ctttcaattc gtttcgcttg attttcagaa taaagaccat
     8161 cgttggttgc tacttcatag taaataccgt tagcatctag aatatctaaa acagagattg
     8221 cttgattatt tggaatcgac acggtaaaca atgaatttcc ttctttatca aatacctgtg
     8281 ccccgttcaa agtgatcatc gcacaatcga ttcctacctc atctaaagct gcgcgagctt
     8341 cttgatagtt tcgaccagta gcgaccatga actcaacacc ggcatcattt gcaaaacgta
     8401 tcgcctcggc attttcagga gaaacactca tatgggcatc taataatgtg ccgtccatat
     8461 ctgatgcaat taatttgatc ataatgatta tcgttccttt ctgcttcttg ttcctagttt
     8521 accatattaa tcaagtgctg agggtgaaga ttgtcccttg tgattgaaga aaatgtgagt
     8581 tattcttttt attaggaggt ggagtttttg aaagaaatca ttcataacag ttggcaagaa
     8641 gttttgtcct cagaattttc aaaagattat tatctgcatc taagagaatt tttaaaaaaa
     8701 gaatatgcgt cccaaaagat ccatccagat atgtatcata tctatgaagc attggaattg
     8761 actccttatg aagaagttaa agtggtgatt ttaggacaag atccttatca tggagaaaat
     8821 caagctcatg gcttatcttt ttctgtgcaa cctggcgtaa agatcccgcc atcgttaaga
     8881 aatatctata aagagcttta tgatgatctt ggaatcgcac ctgttcaaca tggaaatcta
     8941 gtgtcatggg ctaaacaggg agtcctttta ctcaacaccg ttcttaccgt tcgggaaggg
     9001 caagcgtatt ctcatcgcgg gaaaggttgg gaaagattaa cagacacgat cattgaaaaa
     9061 ttaaatgaac gggaaaaacc aatcgtgttt atcttgtggg gaaaaccggc acaagaaaaa
     9121 atcaaaatga tcgataagag tcgccatatc atcataacat ctccacatcc cagtccgtta
     9181 tctgcaagtc gtggcttttt tggttccaaa cccttctcga aaacgaatga tgcgctcctt
     9241 gcgttaggag aagaaccgat tgattggcaa ctgcctgaaa cagtgtaata aaacagcgct
     9301 ttcctgttat agtgcagttc gctttgtatg tgaaaaactt aatttttcca gcatttttga
     9361 ctgatacaga taaaataaac tgttcttttt ttctggaata gtgtatgata atacatataa
     9421 aaacgtctgg aggtcatctt gtggaattgt ttgatagttt aagatttaaa attgttcgcc
     9481 gtgggatcaa aatcgttttc ccggaagcta ctgatgctcg tattttaggc gcagctgccc
     9541 ggttgaaagc agaagaactt gtggaaccca ttttagtagg gaacgaagaa gaaataagaa
     9601 atgcagcgca tgccagagga atcaaaacat ctagctttac gatcatcagc cctgataatt
     9661 acgataagtg ggatgaaatg gtggatactt ttgttgaacg tagaaatgga aaggcatcca
     9721 aagaacaagc agaaaagatt ttaaaagatg tgaattactt tggtacgatg cttacgtata
     9781 tggggattgc agacggaatg gtctctggag cgatccattc gactggcgac actgttcgcc
     9841 cagcacttca gatcatcaaa acaaagcctg gcatcagtcg gacaagcgga gcgatgatca
     9901 tggtacgtgg aagagatcaa gaaaaatatg tcttttcgga ttgtgcgatc aacgtgaatc
     9961 caactgcgca agaattggct gaaatcgccg tagattctgc aaaaacagca gaattgttcg
    10021 atatcgaacc aaaagtagca atgatgagct tctcgacaaa aggctcagcg aaatcgccag
    10081 aagtagacaa agtagttgaa gcaactaaaa tcgctaaaaa actagcgcca caatacatga
    10141 tcgacggcga attgcaattt gatgcttctt atgttccttc agttgcacaa ctgaaagcgc
    10201 cagattcaga agtagctggt caagcaacag tctttgtatt tccagaattg cagtcaggaa
    10261 atatcggcta taaaattgct cagcgtttcg gtaactttga agcaatcgga ccaattctac
    10321 aaggcttgaa caaaccggtt tctgatttgt ctcgtggagc aaatgaagaa gatgtatata
    10381 aattatcgat tatcacagca gcacaaacat tgattgatta acaaatacac gcgaactagc
    10441 caaaaacagc agctgctgtt cttgactagt tcattttttc tttaaaaaaa cttcattacg
    10501 cgatatactg gcaaaaagaa agaagagcgg gagaagatga aaaatgatcg aactatcagg
    10561 attaacaatg acagaacaat tcgcagaggc aattggtcat tccgcaatgc caggtgacaa
    10621 tctgatattg actggtgatc taggtgcggg taaaacaacg ttgacgaaag gaattgccca
    10681 aggattaggg ataacgcaaa tgatcaaaag tccgacatac acgattatta gagaatatag
    10741 tcaaggtcgg attccgttgt atcatatgga tatttatcgg gtagcagcta gcggagcaga
    10801 tttagggtta gatgaatatt ttgaaggtga tgggctttcc gttgttgaat ggggaaatct
    10861 attagaagaa gcactgccgg aggattattt ggaactgatt ttagaaaaaa gtgatacaga
    10921 tttagaaaag cggtatgtga aactaatctc atacggggag caaagtgaaa ccttcaaaca
    10981 gcgaattcta gaaaaatggg ctgaaaatca tgagtgaaga agttgggatc atcttgcggg
    11041 aagcagtgcc aggagatgca aaagacattc ttttgatgat gggacaagtt aataaggaga
    11101 cagaattttt agttttggat gaagcagaac tcctgttacc accagaaacc ttagaagaag
    11161 agttggatta tatatacgaa agcaataata atttattgct gcttgcaatc tatgaaggga
    11221 cgattattgg aacagcatct gtcaaggcag acagtcaatt tcgtttgtct cacgtgggag
    11281 aagtcggtat cagtattctg caggaatatt ggggaatggg actaggcact cttatgctag
    11341 aagaaatcat cagttgggcg aaggaaatgg gtgtactttt tcgattggag cttgacgttc
    11401 aagttcgtaa tgaaagagcc gtacaccttt accgaaaaat gggcttccaa atcgaagcgg
    11461 tcatgcctag aggggcaaga acagatctag gtgaattttt agatgtatat aaaatgagtt
    11521 atttgattga atagaagaaa taggaaaaga gtgagaaact gtcatccttt tgagaaggca
    11581 gaaaaacaca cgaagggact gtgacaaaag tcgtgtcgca gtccattttt tttgaataaa
    11641 cggtgttcaa tcagctgacc ttcggtatta gctcaaaaaa cggcaaaata tgaggaacta
    11701 ttttgccgtt tgtttctcta atactcaggt caaaacgctg atttcacaac ctcttggtaa
    11761 acggtgtcca aaagcttgca tctgcggtaa taagtccaac caaatgaaaa atatgagaag
    11821 ctatttttgt ttggctgttc ttattacttg atgcagaaca gctttttcac aacctctcca
    11881 ataaacggtg ttctcctgcc agctcctcgg tcaccagtca caatatttaa taaacatgag
    11941 aagctatttt ttgaaattgc tccttgtgac tcggagtgaa acggcaggct cacgacctct
    12001 ttgtgtgttt cataagtatt cttttacttt atagtcacaa gttttttcaa tggatctgtc
    12061 ccgaattttt tttcttgata aagcaggatt tccgcacatg cacgactatc ttccaatgca
    12121 tcatggtgat gatctaattg gatgttcaaa ttttcgcaca cagtgttcaa tcgatgattt
    12181 ggaaattcag gaaacaggcg acggctactt ttcactgtac ataaagaaag ataattaggc
    12241 tgttcaagat gataataatc taagcatccc gctaagacct ttgtatcaaa agcagcatta
    12301 tgtgcaacga ctaaacgatt tttttgaaaa tatggttgga tagtttgcca gacttctggg
    12361 aatttcggcg cattgcgtac gtcttcttga tggattccgt gaatttggac atttcgccag
    12421 aaaaaaggag tctcaggctg tatcaatgta tagaattcat caacgatctg gctatttttt
    12481 accatgacta gcgcaagaga acaagcacta taaggctgat gattggcagt ttcaaaatcc
    12541 atggcaacaa aattcatttc tgtaaaacct ccttgggaaa ttactgtaac atacttttga
    12601 aaaaagcaaa taaatttaac aaaaaacata gaatttttta tgtaatattt gttgagaaaa
    12661 gctagttttt gaactcattc cttgtgattt ggagttagat ggggatcatg acttctcggt
    12721 attaagtcaa atttatgcaa agttgtgagg aaccattttc aaaaaaattt cttacgactc
    12781 gaaagtaggc gactttttca caacttctcc actaaacggt gttcaatcag ctgaccctcg
    12841 gtattagctc aaaaaatagc aaaatatgag gaactatttt gccatttgtt tctctaatac
    12901 tcaggtcagg acgctgattt cacaacttct caataaacgg tgttcaaaag cttgcatctg
    12961 cggtaataag tccaaccaaa cgaaaaatat gaggaactat ttttgtttgg ctgttcttat
    13021 tacttgatgc agtgcagctt tttcacaacc tcttaataaa cggtgttcaa aagcttgcat
    13081 ctgcggtaat aagcccaacc aaacgaaaaa tatgaggaac tatttttgtt tggctgttct
    13141 tattacttga tgcagtgcag ctttttcaca acctcttaat aaacggtgtt caaaagcttg
    13201 tatctgcggt aataagccca accaaacgaa aaatatgagg aactattttt gtttggctgt
    13261 tcttattact tgatgcagga cagctttttc acaatctctc gataaacagt gttcaaaagc
    13321 cagtttctcg gtattatgcc aaatttatgc aaaatgtgga aagccatttt cacaaaattt
    13381 ttcttaatac tcgaaactaa acgacttttt cacaacctct ttatttcaaa tctaattcta
    13441 ctggacaatg atcactgccc ataatgtccg tatggatctt tgcatcagct agtcctggct
    13501 ctaaatcttt gctcgtcaaa aagtaatcga tccgccaacc agcattgttt ttccttgaat
    13561 tgaatcggta gttccaccaa gagtagacac cttcctgggt tggataaaaa taacggaatg
    13621 tatcgataaa cccgttatcc aacagtcgag aaagagccgc tctttcttct ggagtgaatc
    13681 cagcattttt ttgattcgtt ttccagtttt tcaaatcaat cttttgatga gcgacattta
    13741 gatccccaca cacgatgact ggtttttgtt tttttagatt ttccaaataa ttataaaagg
    13801 cttcttccca ttctaatcgg taatctaaac gttttaattc gctttgagaa tttggcgtgt
    13861 aacaggttac aagaaagaaa tcggaatatt ccaaagtaat cagacggcct tctgtatcat
    13921 ggatatcgat acccattccg taagtggcat tcaatgctgg ttttttagca aaaatcgctg
    13981 tgccggaata gccttttcgt tccgcgtaat tccaatattg ataataacct ggaagatcta
    14041 aatcaatttg tcctgcttgg agtttcgttt cctgcagaca gaaaaaatcg gcgtctaact
    14101 catgaaaaac ttctaaaaaa tttttattta cgatggcacg aagcccgttt acgttccaag
    14161 agataaattt caataaaagc acactccttc gaattaattt ccttaccttt gcgatactat
    14221 tgtacctcat tttagtaaaa aatggtgtaa gatgaaagta actaaatgat ccaggaggaa
    14281 agtagatgaa gattggtgta atcggtgtag ggaatatcgc agaaaaagca tatttaccaa
    14341 cttatgcgaa aaaacaagga acacttgatt tttattttgc aacaagaaat aaagaaacaa
    14401 aagaaagaat tcaaaaagag tacgggttcc aacatctata tgaaacgatc gatgagcttt
    14461 tagaagaaaa aatcgaagca tgtatgatcc acgcagcaac aaaagtacat tatgaattag
    14521 ccaaaaaatg tctagaaaat ggtatccatg tttatattga caaaccactg agtgtccatc
    14581 taaatgaagt tcgtgaattg caagaattag ctgataagaa ccaaataatt ttgatgatcg
    14641 gtttcaaccg gcgctttgct cctatggtag aagaattgaa acgtatccca gataagcggt
    14701 tgatccacct gcaaaaaaat cgaatagcgg caaaagaaac gacagagttc gtcctttatg
    14761 atttgttttt acatcttatt gatacagctg tgtatttatt agatgaaccc attttgagaa
    14821 caaaatacca gataagagaa acaaaggaat ttttggaata tgccatttta caattagaaa
    14881 cacaaaatca aacggctatt ttaacgatgg atttagcaag tggcgctaat atagaaaaat
    14941 atcaagtaac aagtaaaaat ggtacttatg aagtaaacga tctgacccag cttgtcattc
    15001 aagatgctga tggaaaaaaa gtgaaagaat ttggcgactg gacaaacacg ctagtcaaac
    15061 gcgggtttga gccaatggta gaggccttct tttctcagat ccgtttaggt aaatcggaaa
    15121 cagaattgct taaacaaaaa gaagttgtaa gaagtcatga attatgtgag gaagtgctta
    15181 aagatcacta ccgtcatcaa ctttgaacaa gactggcaag attgtattaa gcgtgataga
    15241 ataatgagga cgtttgagaa aagaaaggaa aatcctcagt gaataaagaa gaaatagttg
    15301 aaagcttagc cacgattact atattgtttg atgaaccatt gatgaactat acgtttacga
    15361 aaacaggtgg accagctgat gtccttgcat tcccgaaaaa acaagaagaa gtaaagcaaa
    15421 tcatcgatta ttgccgaatc catgatatac cttggatggt attaggaaat gcaagcaatc
    15481 tgatcgttca agatggcggg attcgcggtg tagtcatcat gctgactgaa atgaaacaga
    15541 ttcatgtcaa aggaacgatg gtcattgcgg aagcaggtgc ctcactgatt gatacgactt
    15601 atgcagcttt ggcagaatca ttgactggat ttgaatttgc ttgcggcatc ccgggaagtg
    15661 tcggtggagc ggtttatatg aatgcaggtg cgtatggcgg agagatcaaa gatgtttttg
    15721 ctgaagtgga tcttttgctt gaagatggaa cattgaaaac tttgacaaaa gacgaaatgg
    15781 tattttctta tcgtcacagt aaagtccaag aattacgtgc tatcgtattg gaagctcgat
    15841 tcagtttgca aacaggggat tatgaagcaa tcaaagcaag aatggatgag ttgaccgaat
    15901 tgcgtcaatc aaaacagcca ttagaatacc cttcatgcgg aagtgtcttt aagcgcccag
    15961 taggacacta tacaggtcag ctgatccaac aagcaggatt gcaaggcctg aaatggggcg
    16021 gtgcacaagt ctctgaaaaa catgctggat tcatcgtcaa tatcgatcat gcgacagcga
    16081 cagattatat tgaattgatt gcgcacatcc aagaagtaat caaagaaaaa tttgatgtgt
    16141 ctttagaaac agaagtgcgg atcattggag aaaaacaagc aatatcagaa acaagcagaa
    16201 gtttagccaa ttaataagta atggaactta aaataagtaa aagagcagga cggtggccgt
    16261 cctgctcttt tttgtggttg tttacgcaaa tggcgcaaat cttgtacatt ttaacacaat
    16321 aaaacaaaaa atatagaata tttcctcata aatttttcca ttttttgcaa tattctcaaa
    16381 aaaaagataa aaaatgagaa taactgatga aaaaacaagc ataatccatc ataaaatcag
    16441 tataggtgaa aagatgaact ttcatctgca tatcaagtaa ttggtatgag gcattttttg
    16501 aggtgtagga tatgaagttg gattacctta tagaagagga agatagagaa aaactgctgc
    16561 tagctagata tttagaattt gagaaaaaga attatgtagt gctgcctaag ttatatgaaa
    16621 cattaggttt gtctaaattt aaaacaagaa attatatcat agaattaaat aaagaattta
    16681 ctgaattcaa aacaaagcca gagatccaaa tttcagaaga agcagagatc catgtcgaac
    16741 gtctgaattt gacggatatc aaaaatttaa gaagaatcta ctttcaacga tcaaaaattg
    16801 ctcgactgtt aacggagatc gtggattcaa aagtaacgat cgaaaaattt gcagagaatc
    16861 attttttaag catatcaaga gcctatgtaa aaagaagaga attagtctct tttcttgaac
    16921 agcatcaaat aaagttaaag aaaaacatat tagttgggga agaaataaac atacggaacg
    16981 tattatttgg ggtatatttc ggggtcttta atggtttttc tatgccattt agagaaagcg
    17041 tcatgaatga agcaaagagt atcgtgcaat attttgaata tctgttcaat ttaaaactgt
    17101 ctgcaacaaa acgaatcaaa ttcaatttgc taaccactat tagtttgttg agggcctatg
    17161 ggaatcaacc aatcggaaaa cgtttctttt cagatgaata ctttaagagc tcagaaggaa
    17221 tgaagatcat caaggattta tcacatattt cttttcttca tatccctaaa gagcagatca
    17281 ttgatgagat ttcttatatc atgttgtttt tgatcacgga agaagaattt gaattagatg
    17341 gcgcatttgg acttgcggaa gctcattttc atgatatcca catgattagt caaaaaatgt
    17401 cagtggaatt gattgatcaa ttaccatttt cgattgaaat ctctgaagaa gaaagactat
    17461 atatacgaaa agaacttcgt atgggtatga tgcatgtcaa tcgaaaaaat tggatattcg
    17521 actttgagat gactagcttt acgtctaaaa agcagctaca gtttttcttc gagacgtatc
    17581 caatattcag tacagcaatc caaaaggtca tacaagagaa taaacgctat ttcttgggaa
    17641 aagatgaacg tttcatgatt cgcttatttt atgattatct ctttttattg gtcggtattt
    17701 gtccggtatc atattttgaa gcaccgattc atgtctgtat tgatttttcg caaggatctg
    17761 cttataccga ttacattatt tctcaagtga aaggatttaa aaattacaat attgtgattg
    17821 agagtcgaat cacttctcgt acgaatattt atttatctga ttgtttactt gaaaaattta
    17881 ataaagaaca gataatttgg aaaaatccac cgactccaga tgactgggaa tattttggaa
    17941 ataccatcat cagagaaaaa aatagacatt taactgaaaa aatcagctag tatttatgga
    18001 tggcagcttt gtctcacata tttattagct gatttatgtt tttttcaaag aaaaggtaac
    18061 tagatataac tagatataac tagatatatt acttaacaaa attaaaaagt acataagtta
    18121 ttattccgat tttcttgaaa caataaaaaa tcaaaaaaaa tactctccta atagtccatc
    18181 ttcttttgat tttatttata taatgaaatc atcttatgaa aattactact tcatgtttag
    18241 acaagagtag ggaggactaa gaataaatgt gttcaaaaag acgttcaaaa caactaaata
    18301 aacacgcttg tcgtttagat aaggaacggt cgtgttctga aaaacactac gttaagttta
    18361 tgatgcgcac gatgctgatc agtttatctg taggtgtcta tatgcatgta cccgttcagg
    18421 caacgacaca aaccatcaag gtcgtacgta cctctgtttc tgagaccact gattccccta
    18481 tcactattac tcatactttc actgatgcaa atggaaaaca gctacttcct gcattgactt
    18541 cctataataa agtcagttat cctccaacga taaaccacta ctggttaatt cctaacaaaa
    18601 cattatggac atcggaaacc ggaagcagtg tgccgctttc tatcttttcc gatatgactg
    18661 atcctacaaa aggtgatcag ttaaaacaga tcactgatat tttgaatcat gctttgacag
    18721 atataaagga cggtggaagc gcacaagttt cttatgtata tgaaaaagat tattcacagt
    18781 ttgaaagaaa gaaaatcgat gttctggcag ggacaaaagt ttcgatatct gatctggtag
    18841 aaaggctgaa gaacgcagat ggaacacaag gagatatgtc caaagtaatt tcaaacaatg
    18901 ggaatatcgt ggatacgact aatacaggaa catctactat ttccttgtcc tattttgatc
    18961 caattgcttt aaaaacaatg actgcatctg ctgtgatcca tgttcgtgct gatcaaacag
    19021 ctatcaaagg aaaagatctt cacttaagaa caggagagag ttacgcccct aaagatcttg
    19081 ttgaatatgt aacaaaaagc gatggaactt caggaaataa agaagaaatc aaaatgaacg
    19141 ggacagtaga tactacaact ccgggagaat atcccgttgt attaacttat atagactcga
    19201 aaacatcaaa tgtggcagaa acaacagctg tagtttatgt agaagcatct gctgcttcat
    19261 cgactgtcat cactcatttt atagatagtc aaacaaataa agaacttcaa gcagaaacac
    19321 atatgagtag tgtaaatcgt attcctgatt tgcgcctacc tgctgaattt tctgatcgag
    19381 tgccaaatat agatttatcg acttatgaac aaaatgggaa agtcacttct atttctgatt
    19441 ggatgcaagc aaacggatta gacagaaaaa gtcactggtc ggagatcctt gaagtacttc
    19501 actcagaact gatgaataat aggaagcaat caggagggga aattaccttt acttatgttt
    19561 atataccaga caatcgttca ttagaaggtc atgatcttat tgttcatcca aacgaaaaaa
    19621 taaacaaaga agagttgatc caatcagcaa caaattcctt aggtaaaccc gtaccgttaa
    19681 aggatgtatc ggttacaatc aatgaagtac cattggagga tggttatttg accaaacaaa
    19741 caggtgattt tacgattatg tatagttatc atgacccgta ttcactccaa gaactccatg
    19801 ctcagtcact attacatgtt ttaccattaa acaaacagat agatcaaaaa gaacaaaaaa
    19861 gacaagaaga aagcttgtca gaaaaaaaac aacaaatggc tcatgcagtt tctccaacag
    19921 ggaaagtaaa acaaacaaga agcctagagt tagcaagaat atctagaaaa atctcagaat
    19981 ctaataaaac gataaggcaa gaagctcatt tggccccaca agtttctgga ttggaaaatg
    20041 aaattttaga gcctaaaata aaacaacaat cagaaaaaga atttgttgct aaacagtcgg
    20101 aagatcagaa ggaatcagat gaccagaagg aaacgaagaa aaaacgcttg tcaactattg
    20161 attccaccga tgagcatctg cagccgcaag taccacttcc tagtccggga acacccgcac
    20221 ctggtggagg tattccttct tctgctttag gagatttcat gcgaggtatg gcaggaacat
    20281 gggtatacgc taatcgaaat gaatttgacg cagatgactg gttctgattt atatctctat
    20341 ctgttatagt caaataagta gctgagtcat atgttatttg gcataatgga gacgaggagg
    20401 gatagaaaaa tgttagataa aacgttacct tacgcagaaa tctggatgac aagaccactt
    20461 caacaaaatg ttccaatcac gcctttagcg gaaggttttg aatttcgatt ttatcaaaaa
    20521 gaagatgaat ctgcttgggc ggagattgag acttccgttg gggaatttca aacgacagat
    20581 gaagcacttg agtattttca acaaaacttt gctccttatc ctgaagaact tgaaagaagg
    20641 atgttttttg tagagacaaa aaaaggagaa aaagtagcaa cctgtaccgc ttggcgaaaa
    20701 aaatcaagag agcaagtgta tccggttttt cattggttag cagtgaaacc agattaccaa
    20761 ggaaaagggc tcgcaaaagc attgactgcc agagtcttgc aagaatttcc taccctccat
    20821 acggacggac caatctatct tcacacacag acatggagcc atcaggcgat tgctctttat
    20881 aaaaaaatgg gttttacttt tattgctgaa aatctagacg gcacacctaa tccagattat
    20941 gaaaaagtta tgcaggtttt aagtaaatta gataaataac gaaatggtga aataagtcct
    21001 cagtattggg ataaaaaacg aaatagtcat tcctcatatc ttactttttt gagttaatac
    21061 tatcggataa ctaatggcat gccatacatt caaaaaagaa ggggcagtga caaaagtatt
    21121 gtcactgccc cttctcttgt tttttagcat gttttatcgt ttgatttttt taggaataaa
    21181 attcttgatg aacgtaaatt tttggtaatc gtttcgacta tagaggtaac caaatacact
    21241 gaaaacaact gctccaataa aattaacaaa taaatccttc atagtatcca tgattcccac
    21301 gtctaagtaa ccattttgga tgattgtttt atctgtttta ccatctgcat tttgactttc
    21361 aatgattgtc cgagtgatat ctggaacatg atagacagtg tttccgtctt tacttaactc
    21421 cacactattg atttgctgga tgatcgtatc tttttgcata tcgaattgca gtaatttatc
    21481 tgctgaaaat tccacaaact cccacataac accaactgtc atcgaaaaac agaaggaaac
    21541 aactgctagt aataaaggag tcaagttcat ggtttttgta tttttattga gcaaataaac
    21601 aagggaaaag ccgacacccc cagctaaaaa tccgctgaat gcatgtagaa aagtatccca
    21661 cattgaaaaa tagccataaa aatcacttaa ctctcctaaa actgttgaac taaaaataaa
    21721 agataacaaa atgatttcta gcatatttgg aatctgtatc tttaatatct tttccagaaa
    21781 ataagggatt tcaaataaaa ttaatgtcag taataaaagc attgtatttc cccagtgttg
    21841 gttccagacc tgatccacta gcataataac gacgatgata cgtaataaaa tatagattcc
    21901 gtttgtgatt ctacctgcag tttttgtact gttcgtaaaa aaggttttca aatgattcat
    21961 aaaataactc cttcatgtat atccttaatt atactaagaa gcgtgtgttt accaaagcta
    22021 tatgacttaa agaaaaacag agttgatatt gtgaaaaaaa tcacttaata tagtataagg
    22081 agaaaagggg gatacaaatg aaaaaattag ttgctggttt attaacagcc gttgcacttc
    22141 ttgctatgag cgcatgcggc gcagatcaga aaaatacagc gcagacaagt tctagtacga
    22201 aggaatctgc cgaagtatca tcaggcgctt ctgaacaagt atatacagat ccaaaagaaa
    22261 tgaaagaatc ttatgatgtc gtgattattg gagctggtgg tgcagggatg gcagcagcta
    22321 tcgaagcaaa agatgctggg ttgaatccag taattttgga aaagatgcct gttgccggag
    22381 gaaacacatc taaatcatca agcggtatga atgcttcaga aacaaaattt caaaaagaag
    22441 aaggaatcaa agactcgaat gatgccttct atgaagaaac gctaaaaggt ggtggaggaa
    22501 cgaatgataa agaaatgctt cgatactttg tcgatcactc tgctgatgcg gtcgattggc
    22561 tagatcaaaa cggtatcaaa ctggacaact taacgatcac tggagggatg agtgaaaaga
    22621 ggacacatcg tccgtctgat ggctcggcta ttggtggata cttagtggag gggttattga
    22681 ataacgtcca tgagcgggaa atccctattt ttgtcaacgc agatgtgaca gacattaaga
    22741 aaaatgggga aacggtcaat gaagtgactg tacatgttca aggagaagaa ccaaaagagg
    22801 tcaccggaaa agcaatcgta gttaccacag gcggttttgg tgccagcaaa gaatttattg
    22861 aaaaatatcg tcccgattta gctgattata ttacgacaaa tcaagaagga tcaactggag
    22921 atggtatcac catgattgaa aaattaggtg gacaaaccgt tgatatggat aaaatacaaa
    22981 ttcatccaac tgtgcagcag gatgaggggg tcttgattgg tgaggttgtt cgcggagaag
    23041 gagcaatctt ggtcgatcaa gaggggaaaa gatttgtcaa tgaaatggat acaagggata
    23101 aagtatctgc agcaattaca gcattgcctg aaaaatcagc ttatttgatt tttgaccaag
    23161 gcgtgcgtga tcgtgccaca gctattgaat tttatgacca aaaaggatat gtcacaactg
    23221 gaaagacaat ggaagaatta gcagaaaaaa tcgatttacc aaaagaaaca ctagcaaaaa
    23281 cagttgatgg atggaatacg gatgttgcgg agaaaaaaga tgctcaattc aaccgtgcca
    23341 cagccatgga acatgactta tcgtctccga attattatgc gatcaaaatc gcaccaggaa
    23401 tccattacac gatgggcggt gtgaaaatca atacgaacac tgaagtatta gataaagaaa
    23461 accagccaat tagggggtta tatgctgctg gtgaagtcac aggtggctta catggaaaca
    23521 accggatcgg tggaaattca gttgcggaga tcattatttt tgggcgacaa gcgggcatac
    23581 aagcagctcg atttgcttcg gaaaatgaat gaaatagaac gtaaataaaa agaggttgtg
    23641 aaatcagcgt tttgagctaa taccgagggt cagctgattg aacactgttt agtgaagagg
    23701 ttgtgaaaaa gctgccctgc atcaagtaat aagaacagcc aagtaaaaac agcttcttat
    23761 gtttttaact tggttggact tattaccgca gatgcaagct tttgaacacc gt
//
LOCUS       DAXWOO010000029        23532 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.029, whole genome shotgun sequence.
ACCESSION   DAXWOO010000029 DAXWOO010000000
VERSION     DAXWOO010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.029"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            69..1496
                     /locus_tag="ACGE70_000903"
     CDS             69..1496
                     /locus_tag="ACGE70_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8692556.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1588..1953)
                     /locus_tag="ACGE70_000904"
     CDS             complement(1588..1953)
                     /locus_tag="ACGE70_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFF8692557.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1950..2315)
                     /locus_tag="ACGE70_000905"
     CDS             complement(1950..2315)
                     /locus_tag="ACGE70_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFF8692558.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2507..2755)
                     /locus_tag="ACGE70_000906"
     CDS             complement(2507..2755)
                     /locus_tag="ACGE70_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692559.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2754..2882
                     /locus_tag="ACGE70_000907"
     CDS             2754..2882
                     /locus_tag="ACGE70_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFF8692560.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2939..4633
                     /locus_tag="ACGE70_000908"
     CDS             2939..4633
                     /locus_tag="ACGE70_000908"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFF8692561.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4700..5029
                     /locus_tag="ACGE70_000909"
     CDS             4700..5029
                     /locus_tag="ACGE70_000909"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692562.1"
                     /translation="MFLIWLDLLPQMQAFEHRLPRGCEKAALHQVIRTPKLKLLLMFL
                     TWLDLLPQMQAFEHHLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQAFEHRLP
                     RGYEKGV"
     gene            5407..6540
                     /locus_tag="ACGE70_000910"
     CDS             5407..6540
                     /locus_tag="ACGE70_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFF8692563.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6571..6891
                     /locus_tag="ACGE70_000911"
     CDS             6571..6891
                     /locus_tag="ACGE70_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFF8692564.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6920..7384)
                     /locus_tag="ACGE70_000912"
     CDS             complement(6920..7384)
                     /locus_tag="ACGE70_000912"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8692565.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7515..8768)
                     /locus_tag="ACGE70_000913"
     CDS             complement(7515..8768)
                     /locus_tag="ACGE70_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFF8692566.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8797..9114)
                     /locus_tag="ACGE70_000914"
     CDS             complement(8797..9114)
                     /locus_tag="ACGE70_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFF8692567.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9140..9460)
                     /locus_tag="ACGE70_000915"
     CDS             complement(9140..9460)
                     /locus_tag="ACGE70_000915"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFF8692568.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9924..11339)
                     /locus_tag="ACGE70_000916"
     CDS             complement(9924..11339)
                     /locus_tag="ACGE70_000916"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFF8692569.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11356..12672)
                     /locus_tag="ACGE70_000917"
     CDS             complement(11356..12672)
                     /locus_tag="ACGE70_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFF8692570.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12822..13520)
                     /locus_tag="ACGE70_000918"
     CDS             complement(12822..13520)
                     /locus_tag="ACGE70_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFF8692571.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13809..14802)
                     /locus_tag="ACGE70_000919"
                     /pseudo
     CDS             complement(13809..14802)
                     /locus_tag="ACGE70_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
     gene            complement(14883..15716)
                     /gene="msrA"
                     /locus_tag="ACGE70_000920"
     CDS             complement(14883..15716)
                     /gene="msrA"
                     /locus_tag="ACGE70_000920"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFF8692572.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDTRENISVKEQ
                     LSKLEVTLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDTDYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQDERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15950..16693)
                     /gene="truA"
                     /locus_tag="ACGE70_000921"
     CDS             complement(15950..16693)
                     /gene="truA"
                     /locus_tag="ACGE70_000921"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFF8692573.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYRS"
     gene            complement(16884..17519)
                     /locus_tag="ACGE70_000922"
     CDS             complement(16884..17519)
                     /locus_tag="ACGE70_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFF8692574.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(17666..18886)
                     /gene="sstT"
                     /locus_tag="ACGE70_000923"
     CDS             complement(17666..18886)
                     /gene="sstT"
                     /locus_tag="ACGE70_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFF8692575.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEPTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            complement(19228..19905)
                     /locus_tag="ACGE70_000924"
     CDS             complement(19228..19905)
                     /locus_tag="ACGE70_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFF8692576.1"
                     /translation="MGLAFLLAGCTNGNESKKESTKETAKATIATTTTTSTESKPTAA
                     STETADTSTSSESSKTTETTQTSTSSSSVEASSSESSEQVEQTLWNADKSAQLEAFMA
                     TWGQTMGQQYKSYTNQMSVDLYGLKVPQVILNGEWKMAIGGVPVSAEWSESGTGQADY
                     QITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNETANNELKNG
                     FNQIVYG"
     gene            complement(20156..20698)
                     /locus_tag="ACGE70_000925"
     CDS             complement(20156..20698)
                     /locus_tag="ACGE70_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFF8692577.1"
                     /translation="MNIVEEKFAQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLKQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(20703..20930)
                     /locus_tag="ACGE70_000926"
     CDS             complement(20703..20930)
                     /locus_tag="ACGE70_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFF8692578.1"
                     /translation="MKKMIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSTKVKEA"
     gene            complement(21009..22889)
                     /locus_tag="ACGE70_000927"
     CDS             complement(21009..22889)
                     /locus_tag="ACGE70_000927"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFF8692579.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIIIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDNVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDQFSQYYTPTVLLIALLVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQRGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAMLKQAGAKHLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLH
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSSFGSLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLIGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            23175..23510
                     /locus_tag="ACGE70_000928"
     CDS             23175..23510
                     /locus_tag="ACGE70_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFF8692580.1"
                     /translation="MGVEAVDKYLYLLAGNKIQKSLMDFIQELECTFHKKFTHSILLK
                     LLIHTACLIERTLINGHELKIISEDDTRPSHETIFHVKKAFKNIETEFGITVSYDECF
                     FIYDIIASK"
BASE COUNT         7301 a         4829 c         3973 g         7429 t
ORIGIN      
        1 tttcaaaaag aaaacatgtc ataatatttc ccatattgtg caaaaaaata caaggcggag
       61 gctctactat gattgaagaa tatattgaga aaaatatttt aagacagctg tttttatgtg
      121 ggcaattcta tgtaaataaa gaagtcaatc tagaaaagct ctccaatctc ctgcatgttt
      181 gcaaaaccac tttactcaat gatatcaata acataaaaaa agaatttgaa gaacagatcg
      241 cttatacaca tcgggaaaag gaccgttaca ctttatattt ttcagaacat atacctcgat
      301 gcaagatcat gcagcaattg tctcaaaatt cgttgttttt aaaaacttgt ctgctttatt
      361 tggaagaaga tgaacctgat tatcttcaat taactgaatg tgaattcatc tctgtcagca
      421 aagcttattc attaaaaaaa caagtattag cttattttaa tgactgcggg atcgaaatcg
      481 accgctactc ccctcgattc actgaaatgg aaagacgtct gctgcttctt aatgtgtcgt
      541 atcgtttagg cggttttaat tcttgggagc tacctgaatc gttttttgaa cgtgccgatc
      601 gtttcattga atccgtaacg gaaaatagtg gcagatttta tgataaagaa aataaagaaa
      661 tcttatcgat tggttttgct atcagtttcc tacgccagca agtgtgtgct gtgacaatag
      721 attcaaaatt tatcgaagaa atcaaaaaac gtcctattta caattatgtc gaatcagcat
      781 gggaaaacac tgatttccaa acctattata aaaaagaaga attcgccttt attttgacac
      841 tattcaatct ttgtaattat ggtttccatt cttatcagtt gattgcggaa gactttcaac
      901 agctacacca agtgtttatt gacaatactc ccgaaataaa agaactagtt gctacctttg
      961 aatctcactt caatcaagaa ttattcggaa accaaccgtt cgaacgtgcg ctgattcatc
     1021 tgatgcgttc ggcttgggat aactatcaat tattcatgcc agaaaaattt tatcttttaa
     1081 atgaggaaca gacaaattta tataaagaag tccagacaat tttttccagc tggtccagcc
     1141 agttacctta tgatctacgg ttgaatccta attgcatgcg ggcttttgtg atcgaattga
     1201 gcggaattct ccgtctgaca aaagaacatc tgactatcta tatcgtcaca aattcggatg
     1261 ttcattatct gatttatcgg gaagccttgg aagctgtaac tactttcgat tttcaagttg
     1321 caccaacaat ttattcaagt atatctgata taaagaagta tgcgcagcag tcctcaaacc
     1381 gggtattatg tgaacgtaca ttatatacac cagatgcggt ccaatatgaa aatattattc
     1441 ctatttcgat caatacgatc gatcgagcta tcatttcagc tgtgcaaaac aaataagggg
     1501 ctgtgacaaa aatcatgtca cagcctctta tttgttgaag atgcccattt ctgtaacaat
     1561 ctcttgatac acgtcaggct cacaacctca cttattttgg acatctacgg gtccgatctc
     1621 tttatttaaa aaataagaaa tgatggttcg gataactacc aacgccgcta gttggaaaat
     1681 atcctgcaag gtaggtttga taatcgattc gatgatccca gctgaaacaa gaatttccaa
     1741 acttagaagt atatagcctc ccaacatctt tttaatagca ttgttctcgc tgactcggtc
     1801 gctttgcact tttgtatgga caaataaata tttcaaaaaa tccttgattg atagacaaac
     1861 accccaaacc aataaacaga tagcaaagat atcaagtccc agtacaagtt tttcgaaata
     1921 tggaagaaga aactgcatca actctgtcgt catcctacct ctcctcttct ttgtttttct
     1981 ttgtcttcta tttcctgatt caagaaataa gaaatgactg ttcgaatgat aataataagt
     2041 gcaagcttca aaatatctgt ccaagtcggc ttgataactg actcgatgat atcagcaacg
     2101 actagtactt ccaagcttaa caaaatataa cttgctagca gttttcttgt atctgcatta
     2161 cgagtattac gttctgcata gtttttcttg acatgagacc aatcaaaaag tttgaataga
     2221 acaagaacga tcccgacaat taagataccg atagaacaaa tatttaaaat agcaatgatc
     2281 cattctagaa attcaattaa atttgagcga aacatatacg ctacctactt tcttttaatt
     2341 tgttcctatt cttttattgt aaaagaaagt taccttgttc gtctatttaa aagttgttct
     2401 aatgataacg gttcgcttaa atttttcttt ttcactctcc catttttctc cctccatcca
     2461 taaatcgtgg aagtatccaa aacaacgtag caagttaaaa taaggcttag tccgtcatca
     2521 gcttttcaaa gaagcactgt ctcggtgctt tttttgtacc caaatttcta aaaatagaac
     2581 catacacctc ctcaaatctt ttcttcttac ctaccctatt tttatttaca ggaaaatcag
     2641 acacttcttc ttttttgtca aaatttcctt tttcttctcg tcctatcatt tggcattcta
     2701 ataaaaacga actatccttg gatatagaaa gagaaaacaa ggaggcgttt ttcatgagta
     2761 aaaaattaat caaagtcggc atcggtttag gtttgcttgc ccttggcgct gcatatctag
     2821 gaaaaaagac aggactattc gaagatgaca gtcatttata tgatgaattc gaatcaatct
     2881 agcatcctat ttagttgaat cactacggac atacttggaa gacattggag gaatcacaat
     2941 gagaaaaagt aaagcaataa tgattggtgc cggtattgcc aatatggcag cggctgttta
     3001 tttgattcaa gaaggcaaat ggaaagggga tcagatcacc ttttattcat tggatgatca
     3061 cggttcaaat gatggtgcac cgaccgacac tgtagtggat gaatattgga ataaaaacca
     3121 tccattagaa aaccaaaaag gctatattgc tcgaggcggt cgcatgctta attaccgtac
     3181 ctatgtagat ttgatggatc tgttaagtcg aataccttct gttactgagc cagggatgac
     3241 tgctgaagaa gatacgagag attttgatgc aaaacaccga acctttgaca aagcacgttt
     3301 gatggaaggt ggcaaaggaa tcattgatgg cggaaaactg ggattcaata ataaagaccg
     3361 tattcttctg actaaactga ttgctatgcc tgattcagaa gaagaaaaac tagacaatat
     3421 cactatcgaa gaatatttca aagatgatcc acactttttt gaaacaaact tctggtttat
     3481 gtgggaaaca acatttgctt tcagaacacg tagttctgct caagaattgc gtcgctatat
     3541 gcatcaaatg atttatgaat tcactcaaat cgaacatcta gtgggcgtca atcgaacacg
     3601 ttacaatcaa tacgaaagta ttatgcttcc tttgatcgag tacttgaaag aacaaggatg
     3661 caaaatcatt ttgaaccgcc gtgtcactga ctgggaattc aaagaaacac cgatgcaaga
     3721 tgaaatcact gtgactggtt tgaaaattac caatacactg actcaagaag aagaatacgt
     3781 tgtggtcgat gaagatacag cagttatttt cactaatggg tccattacag attcagctac
     3841 tttaggagat tacgaaacac cagctgtaga aaatatggat tatggtgcag cttctagtct
     3901 ctggaaaaaa gccagtgaac gtttctacaa cttgggaaat cctgacaaat tctttgctga
     3961 tcgagatgca agcgaatggg tcagctttac cctgacgacg aaaaaccatt tgttgttgaa
     4021 tgaaatcacc cgtatcacga ctcaagaacc aggaaatgcc ttgaattcat ttatttcaac
     4081 cacaccgatc acgccgttag gccaaaaaga tgtcaatatg tctatcgtcg tgcatcatca
     4141 accgcatttt acgtctcaga aaccaaatga aacagttatt tggggttatt tcctttatcc
     4201 aagacgacgt ggcgaatttg tggataaaca gtatattaaa atgaatggaa aagaaatgct
     4261 agaagaactg attggacaac tttcaaaagt agatcctgga ccagtgaata ttcgtgaaaa
     4321 agaaacagaa attttcgaca gcgtaataaa taatatccct gtctatatgc cttatgcttc
     4381 cgcgttgttc aataatcgtg ccaaaagcga tcgaccaaaa gtcattccta aacattcgac
     4441 caatctggcc tttacaggag aatttgtcga gcagccttat cagatgatct ttactgaaca
     4501 aagtgctgta cgttctggag aaatcgctgc tttccatttt gcgggtattc ctatgtcccg
     4561 tcttgtcaaa acaccgcgtt acgacaaaga tattcctact ctagctcgtg ccgcaaaaaa
     4621 aatgtttgaa taatcaagag gttgtgaaaa agctgccctg catcaagtaa taagaacaac
     4681 caggctaaaa cagcttctta tgtttttgat ttggttggac ttattaccgc agatgcaagc
     4741 ttttgaacac cgtttaccaa gaggttgtga aaaagctgcc ctgcatcaag taataagaac
     4801 acccaagcta aaactgcttc ttatgttttt gacttggttg gacttattac cgcagatgca
     4861 agcttttgaa caccatttac caagaggtta tgaaaaagct gccctgcatc aagtaataag
     4921 agcagccaag ctaaaacagc ttcttatgtt tttgacttgg ttagacttat taccgcagat
     4981 gcaagctttt gaacaccgtt taccaagagg ttatgaaaaa ggcgtttagt ttcgagtatg
     5041 aagaaaaatt ttgtgaaaat ggttcctcac gtttttgcat aaatttgact taataccgag
     5101 aaactgatcc cttctgtcat aacctctttt tatttatgtc tgtttctttt tgaaaacaac
     5161 tattttttca acgtttactc tcttgccaat ccaacatcat ctaacggata caactatttc
     5221 aatgattacc attacactaa aaaatcggaa acacacctct ccgaacacaa atacataaga
     5281 ctatagaatg tttcatttaa gaaaaaaagc gtttcattat caaaaaggta agatctcctt
     5341 taaaatgaaa ttatctgtag tactaataat gcagacaatt ttatgaatct gaaaggagca
     5401 tctccaatga aagcagtcac ttggcaagga aaacagaaaa tggaaatcag aaacgtagat
     5461 gatccaaaaa tattagaacc aacagatgca atcatccgta tcacagcaac agccatttgc
     5521 ggctccgatc tgcatcttta ccatcatggt gaagcagttt tggaaaaagg gtatgttgta
     5581 ggacacgaac caatgggaat cgttgaagaa gtgggatctc aagttcaaac attgaaaaaa
     5641 gggcaacgtg tggttatccc ttttaatatt agttgtggtc attgtcattt ctgcctgaat
     5701 cacatggaaa gccaatgtga tgaatcaaat ccagatcagt tatatggcgg gcttttcgga
     5761 tttggcaagt tgaacggtaa tcattggggt ggtcaagcgg aatatctaag agttccttat
     5821 gcagactcta cttcatttgt tgtaccagat aatgatcttc ctgatgaaaa agttttattt
     5881 ctatcagata ttttgcccac agcttattgg agcgttgaaa acgcaggagt aaaaaaaggc
     5941 gatacagttg tgattctagg ttctggtcca gtcggtctat tcgctcaaaa attcgctgtt
     6001 atggctggag cagagcgcgt aatcgcagta gatccagtac agcaccgtct agataaagcg
     6061 gctaattaca atggggttga aacttatttc ttagaagaca cggcacaagc tggaacagaa
     6121 ttgtatgaac tggctaaagg tggcgcagat gtcattatcg attgtgtagg aatggacgga
     6181 ttagagcctg tcaaagaaaa agcgaaaaat ttagtaagct tacaaagcgg cacaatctct
     6241 ccattacaga tggcatcgca agctgtcaaa aaatttggaa cagtccaagt aactggtgtt
     6301 tatatgactc cagcttcttc ttacccgctg caagaatttt ttatgcggga tatcgctgtc
     6361 aagcatggac aggcaccaat tatccatcta atgccaaaaa tttacgcaat gattgcagaa
     6421 ggactattcg atcctacaca aatcattact cattcgctgc cactatcaga agcagcacaa
     6481 gcatatgaga tctttgacaa aaaagaagat aaaaatataa aagttgtttt gaaaccttga
     6541 attcagtaaa aaatacaggt gattttcttt atgcccaaaa tcaaacgaaa tacccatgag
     6601 atcgaaatag gaaaagaaga agatatcgaa atacgaggac gtcgcttccg cttctatttc
     6661 caaaatcgtt acacattgat ctcactggct gtcgatttat taaccgggat tttctatatt
     6721 tttggaagcc ttgcttcctt aactaaaatc cccgatcgat atggcaccta tttttatttg
     6781 gctggtgcta ttttcttgac gattcgtcct attctcagaa tcgtaaggaa catttttatt
     6841 tacgacgaaa aaaaagcaat gaagagaaca agcaaaaaaa aagaggcatg agaatttttc
     6901 aaatctcatg cctctttttt caccctatcg tatcactttg atacagacga gcggtcgtcc
     6961 cactttcttc cttgaccatt gtcaaaaatg tccactcgta tcgttcataa aacgtggtat
     7021 gatctgtaat taagtaaagt ttttcgtacc caagttgatg catttctttt ctgatttccg
     7081 ctaatatccg cttggctatc ccctgcttac gcttttccgg ttcaacaaac agggcacaaa
     7141 gatttggtgt cagatcaggg cggttatgga agtcgttttc aatgacgccc gctcctccga
     7201 cgatggtttc tttttcatct aagaccacat accattgagg aatactttcc ttattagcga
     7261 ttccagtacg aatactttcc tcatacacaa cagcaggaac tccccatttt gatgaaaacc
     7321 aattcgcgct ttgttttact aattctgggt gttctcttaa tttgatccag ttatatgtaa
     7381 ccaatgttta aacctccgac taaaataaaa cgtttacaaa aattatacaa gtctttatga
     7441 aaattagcaa tgacaacaat agtgatgtaa aaaaaaaacg gaaaacaatc acttgttctc
     7501 cgttttttga ttaatcaagc atctaaagct tcttttcttc tttcttggat cgtagctacc
     7561 ttgacgaacg gtagatagat caaaatagaa agaaccaaat tgacagcggc tagtacacca
     7621 cctgcaatac tttttgttgc caatacaccg ccaataattg gtggagttac ccatggaggc
     7681 ataacggtag ctgctggcac taaacctgta gaagtcgcaa agtaagcaac actaactaag
     7741 accattggtg tcaaaataaa tggaatgaac atgatcgggt tcaacacgat aggtaaccca
     7801 aacatcgttg gttcattaat attgaagaca cctggcgcaa tcgacaagtt tgttacaacc
     7861 atatatggtt tgtttttacg tcctactaaa taaatagcta acagtaaacc taaagttgca
     7921 cctgttcctc ctaagttgac aaagctgtca aagaaaggtt tgttcacgat aaacggtgcg
     7981 ctatgcccag ctgcgattgc attaacgttt gcttcaatgg ctggcgcatt gattgtttgc
     8041 atcaaaggat cgaccatatt cgctccgtgt aaaccaaaga accataagaa tggtgtgata
     8101 aatgcaagca gtaaggcaga tggataagag tttgcaagtc ccataaatgg ttcttggacc
     8161 actgtataga aagaacctac gatatcatca acaccgaatg cagcaaaaat tgcagcaact
     8221 aaagcaaacg cagagatagt gatcattgct ggtagtaatg aagcaaatgc ttttgcaact
     8281 gccggtggca caccatcagg cattttgatg atcaattttt catttccgct taaacgtaca
     8341 aagatttctg ttgaaataat cccaacaata atagcaataa acaaaccgtt tgagctcata
     8401 cctaatgctc cgcctaaagc gaagaaagag cctaaagaaa tcacacctgc ggaaacaccg
     8461 tcttttcggt agccgttagc taaatgataa gcaagcaaga aagcaatcaa gacagaaaga
     8521 atggcaaatg taccgttcca agcagcagcc ccgaatcctt tccatgattc accaccaaaa
     8581 attgagttca tcaattcttg gaacccggga atcggtaagt tattgatcat tactgctaat
     8641 gctcctaaga tcattaacgg catactcacc ataaatgagt cccgaatcgc aactaaatgc
     8701 cgttgtgcac caatttttga tgctacaggt ataaaatgtt tctccataaa tgcaacaaat
     8761 ttatccataa atcttccctc ttttctttac tgtagattac gcgttcaatc gacgatacag
     8821 atcaatgatc tcttttgcta aatctgtaaa agctatagat gtcatcagat gatcttgact
     8881 gtggaccgtc aaaagcgtga cttcaacgtg atttccttgt gcttcttgtg tcagcatctc
     8941 tgtttgagag tgatgtgctt ggactaaaga ttcctctgca tctttgattt tttcatctgc
     9001 taaagtgaaa tcaccttttt tcgcagcagc aatcgcttcc attgcatcgc tttttgcatt
     9061 tccgccaaac atgatcagac ccatgatggc ttctagattt ttttgatctt ccatttattg
     9121 tatcctccga taatttttat tatttgttta ttagtttttc tgcttggtcg agtacttttt
     9181 caccattcat catgccatag tcagccatat tgattacatc taaaggaatg cctttaggtg
     9241 ctagcttttc agcaaattga ttttgcataa agcgtacttg cggtcctaaa agtaaaacgt
     9301 ctacattttt agattctagt tgattatccg catctgaagc agatacagca aagatgtctg
     9361 cttccattcc ttttgcttct gctgcctttt gcatttttgt gactaggaga cttgtactca
     9421 ttccagctga acatacaagc atgattgttt tcttttccat tttaaacacg ttcctttcta
     9481 tacttacact tgtatataag caagtagcgt gccaacttta aaagcgcttt aaaaagttga
     9541 tatcatcaca tttaaggtct ataccaagtt gcatacacac taaaaaagaa agcgcacact
     9601 aaacacacaa attaattttt attacctttt ttcttttaac gtgcacaaaa aaagttgtga
     9661 cacaaaactt gtcacaactt ttttcaaata aacggtattc aaaagcttgc atctgcggta
     9721 ataagtccaa ccaagttaaa aacataagat gttgttttta ctttgctatt cttattactt
     9781 gatgcaggac agctttttca caacctctca ataaacggtg ttcaatcagc tgaccctggg
     9841 tattagctca aaacgctgga ttcacaacct cttttatttc aaataaccag taaatacaat
     9901 gaacatctac aaaaaacttc tatttattta tagatttcgt caggcaaatc caattctctc
     9961 ttatcaatca aagtctgata ccacgaagca gattttttca atgttctgct tcgttcatcg
    10021 ttcaattcga ttcgaactaa tccatatcga ttcttaaatg cattcattgg tgacacacag
    10081 tcagtgaatg cccataacat atagccttcg caatttgcgc cttcttctgt tgctttgatc
    10141 aagtaatgca aatgctgtcc gataaattcg atacggtaat catcttgcac gattcctttc
    10201 tcatccatat actgttcttc atattctctt cccataccgt tttctgttat cagccaaggg
    10261 atattatgat aattttcttt tagatacatg gctatatcat acatgatttt cggatagatt
    10321 tcccatcccc tggaattatt cattttcttc ccaggtaaat caaagacttc ataatatcgt
    10381 tcaggatgga aaggtgtctc ttcattccat tgataacgag gggcgcgaac gcgtttagga
    10441 taatattgat tgattccaag gaaatctact gtatgatgct gaatcgtttc taaatcctct
    10501 gttgcaggat cgaaataaat cgattgtttt tgacacaact ccaggaactc ttctgtgtag
    10561 tatcctttta ccatcggatc aaaaaatact cgattataaa acaaatcata catataagca
    10621 gctttttggt cttctggaga attggatcga gcatagacca tttcaggatt taaaatacag
    10681 ccaatccttc caccataatt tccgctgcgg taaatctcta ctgctttagc cgttgctaaa
    10741 actttatgat aattccacat catccacttt tttgtgtttt gttcatgcgg ccagcgaaca
    10801 gcatccaaat agcaacgcgt ttggggaacg attggttcgt tgaatgtaaa ccagtttttt
    10861 accctatcac cgtaacgatc aaaggcaatt tgagcatact tagcataaaa atcaactact
    10921 tttcttgaag accagccatc atatttttcc aaaagaaaag ctgggacttc ataatgttcc
    10981 aaacaaatca tcggttctac gtctgcttcg atcaaagagt cgatcatttc acttatatgc
    11041 tgagcatatt cttcatctac tatacagttc tcataatcag taaaaaaacg agcccaattg
    11101 atagaagtcc gataatgtgt cagatggatg gatttcatca gatccacatc ctgctggtat
    11161 tgttccataa aattagttgc tacggctggg ccatacccat tatgccaaac aaaatgttca
    11221 tttttatacc agctatctat aaacgaatcc tgtccttctt tttttccttt ccaaccttcc
    11281 gtttgccaag cagaagatgc agcaccaaga ataaaatttt ttggaatagt aattttcatg
    11341 atttcctcct gttttttaga tcccatcatt ttccacacga tttgctactt ttacaaatgg
    11401 cagatagatc aaaatagaaa caataataca aacaagttgc gtaataactg caccgatatt
    11461 tcctgctgtt gccagataag cattgacgat cggcggcatt ggccaaggga ccattacaac
    11521 tgctttcgcc gcaaatccta aagcggttgc aaaataacca atcgttccag tgataagagg
    11581 cgttaagata aacggaatag ctaaaattgg attcagcatg atcggcatac caaaaataac
    11641 tggttcattg atgttgaaga tcccaggagc aagggataac ttggtgattt gtttcatatc
    11701 ttcccgtttt ccaactaaca atactgcaat cagtaaaccg atcgtgactc cagacccgcc
    11761 catactcata tacatatccc aaaatggcat agtaataatg ttcggtactt cttttccagc
    11821 ttcaaaagct tccgtattta cagcaattgc cgctaaaagc aacggttccc gtatcggctt
    11881 caccatttga tttccatgga taccaatcac ccaaaagact tgcgcaataa acatcaatag
    11941 caaaataccc ggtaaccctt gtacgacacc ttctaatggg cgttgtacga cattgtaaat
    12001 gatatcataa aggtacattc ctgtaagttc tttgatgata aatcccgcag ccgcgatacc
    12061 cgatacagtc agaatcgtcg ggaataaagc agaaaaacta gcagaaacat tcgttggcac
    12121 ggtatcaggc attctcagct gtagtttctt ctgtttcctc aaccagcaat aaagttcaat
    12181 agaaaggatg gagaccaaca ttcccaaaaa taaccctttc gtatctgtat attgtttagc
    12241 caatacgttt tctactataa cagtctgatt ttcattcact aattcataaa cagttggatt
    12301 aacagataaa taagacatca ctgcgatgat accagggaat acaccatcca ctttattcag
    12361 cttggctaat tcgatcccta tcagaaaaac agcatagatg gtcaagaaac ttagtgtgac
    12421 atagctgatg gcagatgcga taggctttaa ttcttctaaa aaacgcaaga aacttatctg
    12481 tgctaagcca ttttccgagt caaagaccat tgctgaaaac aatgtgccaa aagcacccgt
    12541 aataattacc ggaatcaatg cagcaaatgc gttttttatc accatgatat accttaaatt
    12601 attgattctc gttgccacgt tccctaaaac attggctaat ttatccataa aactcattcc
    12661 agatcccctc attcctgctt cttctccttg acgtcatctc tttttgtcta ttagtcagcg
    12721 cttacatttc gatgaacaga aactatttac tcctcttttt taccaaatat aaaacaaaaa
    12781 ttcatctgat gtatttatca taaaatacat cagatgaact gtcaattagt ttctttttct
    12841 cctattattt tctgtaattc tctccgattt attgccatat ggacagtcat tgcatcaaac
    12901 gcagcaagtg cattccgcct ttcgatggcc tgaacgatct ctgtatgaat atcgatcgtc
    12961 tcgattttca gatgctggat atcataattt ttattaaaca aagcaattga ttcattaatg
    13021 attgggataa tatgcagcaa ggctacgttt ccgctagctt ctgcaatcat cgtatgaaaa
    13081 tcgatgtcta attgaacatg ttcttgtgtg ccttctcgaa aagaagtttc aatcttttgc
    13141 gttaattttt tcatttggac gatttgattc ttcgttgcat attccgctgc caaacctgcc
    13201 atcttaggct ccaataaata tctcagttca aataagtctc gtatcatccg atcctgatct
    13261 tcaatcaaag aaaaaccgag tgggtcattg gcaatccctc gcttatcact gacaaatgtc
    13321 cccgctcctt gccgaacttc aaggatattc ctggaagcta gagcccgcac agcttctcgc
    13381 accgtgcttc gccccacctc taaataatcg gacaattcat attcattcgg cagtttatct
    13441 cctacttgca actgcatttc ctttaaatat ctaaggatct tgtcagtagt tacttctact
    13501 agggttttat tttcttccaa accacatacc tccttttcat ctgatgtatt ttttcgtaat
    13561 tattataaca cgtttgaatc atctgatgat tttttgtata caaaattgat taatccgttc
    13621 atagacatac aaacgaagaa gatatttacc ataaaaaaga ctaggataaa attcactcct
    13681 agccttcgat catttttatt ttgaacgaca aaaaatatga tgttcaataa cagacgatct
    13741 tcattcgatt cttgatgtca tcttttgttt tttttaatat atggataaaa agtttgatta
    13801 catcgtttct attctagaat ttggtcgatt ctcaattctt cttctggtga gaaatctaaa
    13861 ttatccatca ttcgaacact ttcttgtaac tgacttaacc gacttgctcc gaccagtaca
    13921 cttgtaacag atttttgtcg caaattccat gcaagggcca tttgcgctaa tgattgtcct
    13981 cgtgatacgg cgattgtttg cagtgctttg attttttcca gagttgatcc aacttgttct
    14041 tctgaaagga atggtatttc cttacgatgt gctctagaat cttcaggaat accatgtaga
    14101 taacgatttg tcagcaaccc ttgagcaagc ggactgaacg tgattgcccc tagcccttct
    14161 tcttctaata ctggagatag atcgtcttct agtagtgcac gagaaaacat attgtagcgc
    14221 atctgatgaa taatgaacgg tgcttccttt ctttttaaaa tttcagtcat ttttttgtat
    14281 cttctccatt atagttggat ataccaatat ataatgcttt tccttgtctt acaagttgca
    14341 tcaacgcttc tgctgtttct tccaaaggtg tatcagggtc tgggcgatga tgatagaaga
    14401 tgtcaacata atctagcccc attctttgga aactttggtc acaacttgat atgatggatt
    14461 tacgagagcc ccattcccca tatggccctt cccacatatg ataacctgct ttgctagaaa
    14521 taatcagttc atcacggtag gcttgaaaat cttctctaaa aatacgccca aagtttttct
    14581 ctgcagctcc agcagggcct ccataattat tcgcaaggtc gaaatgggta attcccatgt
    14641 cgaatgcgcc tcttaaaatc tctctttgat tatggatagg atcataatca ccaaaatttt
    14701 gccataatcc gagaggagag ataaaatcgg taattttaaa cctgaatgac cggatttacg
    14761 atattccatt ttttcataac gtgcactatc tgcaatatac aatttttctc ctcctcatca
    14821 aatcttttta aaacgctttc ttgttttcta tttttagttt actacatttt aaagaaaagg
    14881 agttaatcaa aatgattttt tcgtatattt ttttgccatt tgttttttac tcgttgatag
    14941 atcagaaatt gattacgtgc ttgctggatt tttttatagc gtttcggttg ttttttgtaa
    15001 aattgctgat gatagttttc tgctggccaa aacgtaccgg ctggttcgat ttttgtgacg
    15061 attggttggt ggaaagttcc tgaactgtct agtttctgtt ttgattgttc cgctaattct
    15121 ttttgtcgtt cgtcttgata aaaaatcact ggacgatatt gcttccctct atcttgaaat
    15181 tgtccaaatg tatctgtggg atcagtaatt tgccaataga ttgtcagcaa ctcgctataa
    15241 cttatttctc ttgtgtcata aatgatctcc acagcttcca catgacctgt gtaaccacca
    15301 cttacttgat cataattcgg tttttcaaca tgtccgcccg tgtatcctga caaaacagat
    15361 aggatccctt ttttcgattc aaacggctcg accatacacc agaaacatcc ccctgcaaat
    15421 actgcacgtt cttgatagtc agtatcttgc atggcatgct tggaaaagtc atatttttgc
    15481 tccttatcaa atccttccgt aatcaataaa taaaagtcca ttacatcagg cgtcagattc
    15541 attcggatag ctaatggacg tagtgttact tctagttttg ataactgttc tttaacggat
    15601 atattttctc ttgtgtcatt ctttgtctga atcaatactt ttcgttccca atctcgtgta
    15661 ttcggattca aaatcagatt ataaagtgtt tgaatgaata tttgttcctc atccatagat
    15721 tctcctcctt tccgaagaag acaagctagt gactttagct gtaatttatc ataaagtgag
    15781 gaaataatcg attgaatcgt atttattcaa agaagataag cagcttcaga tgatataaga
    15841 tgatctttat cttaaaaagc atactctatg gtttggttta actaaaaaac gacctctgaa
    15901 aaaagggatg ttctccaatt ctcagaagtc gagtaaagat gattcttttt tatgagcgat
    15961 aataaacttc atccaaaaac agaccttgtg cagggacggt ttctcctgca tgttgccgca
    16021 ctttgctttc aaatatttca tcgattgttt ctatcggcat ggcacctgtg ccgatttcta
    16081 gtaatgtccc catgataatc cgcaccattt tatataagaa tccgtctccg acaaaagtaa
    16141 aatgaagcat ttctcccttt cgttcgatag ataattcatc aatcgtacgc actgtggatt
    16201 tttttgattt tttcaatgca gaaaaaccaa tgaaatcatg tgtacccact aatttttcgc
    16261 aagctgcatt cagcttgtcc atatccaact cttgagggaa ataaaagctg tggtttcgct
    16321 caaatgctga aggaatcacg ttgttccaaa cataatagct gtattttttg cccaccgcat
    16381 tatatcttga atggaagcgc tcaggcattt cttcgattct tttgatgaca atatcccgcg
    16441 gaaggtaacg attcataaag tcgatcattt ctgccagtcc aatttctgtg tttgttttaa
    16501 aatttgctac ttgtcctcgc gcatgggtgc cagcatctgt tcttcctgat ccaataattt
    16561 ctatctcttc atttgtcatc tgatgcaata ctttttcgat ttttccttga atggttttat
    16621 cgtcatctcc aagtctttgc catcctgaat atctttttcc gtcatattcg attgtcaact
    16681 tgatgtttct catattttag tggctcctct gtcttccatt tggtcttctc tctacctttt
    16741 taagattgta gcacaaaata tagctccttg cttctctaag acagaagcta tacaagaaaa
    16801 ctgtggaaat atatatttca gaaaaacaaa aaacagccac cggatgaagt atccgctgac
    16861 tgttcaattt gacggctcaa gttttactgt ttgagatgca agatactcaa ccaggcaatg
    16921 ggcacataat taaagaaaaa actgattctt tggcaaaaac ctattttatt cagtaatggc
    16981 caagaattta tagaaggaat tcttgccagt ccataaatcg ccgcaaaaag caaactgatc
    17041 acagacagtc caaggtataa gtggcttacg tccatttgcc cattttttcg atagatcaag
    17101 aaaagaagaa ctggaaacaa taaaaaacca ctgtaaccta aacctgatcc gatattatgg
    17161 acccatgtcg agacattcca cgaagattca tgggtattga tactgaacaa acctgtaaag
    17221 atacaatcac ctataccata gaacgcgatg gctgctgtaa ctagggtagc taacacacga
    17281 gatgtactta caaatgcagc atacaatgca ggcaatgaca ggatgaaaaa gatccctgaa
    17341 accacagacc aaataagaaa cgcttttctt acaggactgg aaacctctcc aaatacactg
    17401 atcacgctct tcatttgatc taattcagga taatacaatc ctaatacgta cggagtcaaa
    17461 aaatcactca gcacgcctaa taataaaaag aaaaaaccaa atttacgtaa aaaatccatg
    17521 atacgcctcc aataacaatt acctatgctt ttaattgtta ttataaacga ctttatcttg
    17581 atttaacaga aacctctatt tacacgaaca tctatataac tcattaaaaa ctcgaatcat
    17641 tttaaaaata ctaaatatct aacgttcaac gttcacttaa tccatctaaa caatctttca
    17701 atttgatttt ttccccactt cttttacgcg cgcctagctc tcctattgca gtaaacaaca
    17761 gatcacttga cgaatttaag gcagtttcca ttgaatcctg gataacaccg ataataaatc
    17821 caataccaac gatttgcatc gcgacatcat tagagatacc aaacaaacta catgctaatg
    17881 gaatcaataa cagtgaaccg cccgcaacac ctgaagctcc acaagcagct aaagcagaca
    17941 aaatacataa aaggactttc aaaacaaatg gtgcttcaat ccctaaagaa tgtacagcag
    18001 tcaacgtcat caaggaaatg gtaatcgcgg caccgcccat attgatcgta gcacctaacg
    18061 ggatcgatat ggaataagac tcttttgtca atttcaatct ttcagccaac atcatgttta
    18121 tcgggatatt tgcggcagaa cttcgggtaa agaaagcggg aagtgcgctt tctttcaaac
    18181 aaaagaaaat caatggatag ggattttctt taataaacaa aaacgtgagg aatgggtaca
    18241 caactaatgc gacaaacagc atcgtaccga tcaaaacaag taaaagctgc agatactctg
    18301 ccaatccagc gattccagtc gtagcaattg attgataaac caatcctaaa atccctatcg
    18361 gagcaaatgc aataatcact tgtacgactt gtgacaacgc tatagatatc tgatccacca
    18421 actcttttgt cggttctgaa cgtgctcgaa aagctagtcc caacaataca gcccagaata
    18481 aaacgccaag gtaattcgca tcaataagag attgaacagg attttgtgtg acattcgtca
    18541 acatagtaga taaaacactt tccaaacttt gcggtgcttt ttcagatact gtttcttgta
    18601 aagtaatagg aacagtaaaa attgaactta caatgactgc tacaagtgca gaaagcaatg
    18661 ttcccactaa atataagatc aaaataggct tcacgaatgt cttattccca attttatgct
    18721 tggcaattga cgccatcgtc aagaaaaaaa ccagaatagg tgcgattgcc ttcaagcaac
    18781 tgacaaacaa cgtccccaat attgaaataa atgtccacgt ggggaaaaag ataccaaaca
    18841 atattccgac taaaatacca acaacaatac gttgaactaa cgacatatcc aaaaacttct
    18901 ctaacaaatg accacccttt cacaatttta acaatcattg tatcatttta tctaaagaaa
    18961 ctaaaggtgc aattttctaa aaattctaat aatacaattg gcaattcatg ctcttgcaac
    19021 attcaatttt agtattggag ggtttattca atctctaaag aagtcgagag aaaactattt
    19081 atcgttatta ttatagttat ccaagtaaaa cgtaactaag atgaaggacg aaactagaaa
    19141 tttcatcctt catcttaatt cttagacata gcaatcaatt aatattttcc actgagtctc
    19201 ttctttctct cactctcact ggtttactta cccataaacg atttgattaa agccattttt
    19261 caattcatta ttcgctgtct cgttaaagta aagataatta tcgggattcc cctgattttg
    19321 ttgtgtcacc aatacttttg gctgattttg ctgaaacccg aatagataga cgtgcttttt
    19381 cagatatggt tctgtctctg catcggaata gacggctgtg atttgatagt ctgcttgacc
    19441 tgtcccactt tctgaccatt ctgcagaaac aggcactcca ccaatagcca tcttccactc
    19501 cccgttcaaa ataacttgag ggactttaag accatataag tccacactca tttgattcgt
    19561 atagcttttg tattgttgtc ccattgtttg gccccatgta gccataaatg cctctaattg
    19621 tgcggatttg tctgcattcc acagtgtttg ttcgacttgt tcgctgcttt ctgaagatga
    19681 agcctctaca gaagaacttg aagtagatgt ttgagttgtt tcagtcgttt tggaagactc
    19741 tgacgaagta gaggtatctg ctgtttccgt acttgctgca gttggcttgc tctctgtgct
    19801 agttgttgta gtagttgcaa ttgttgcttt cgctgtttct tttgttgatt cttttttcga
    19861 ttcattcccg ttggtacaac cagctaacaa aaaagctaga ccaattgtta ctaaaatcct
    19921 ttttttaaat taaaaaactc ctttcgatta ctcccacttc tttattctat caaataagca
    19981 atgtgatttt attgattttt tgtattttct catccatctt ttaaaccttt taaaaactaa
    20041 gaagcacccg ctgatgttgt ctgtaataca tcagcgggtg cttcttttat tttaatcttc
    20101 gcgattttcc aatttacctc acgctttata tttgtatttg aagaaacata gacttttagt
    20161 catcatcgtc ttcctcatcc aaatcttgtc ggacagtttt tccttggagt ctttgtactt
    20221 ggtagatgat ttctttattg aaacttaaaa gttcaacaag caattgtttc aagccatatt
    20281 tttcttcttt ttcagctaat ttgattgccc gtgtgataaa gacattttgg aaatcaaaat
    20341 cttttgcaaa gttttccaac attgttgagg ctggtaaata tttgttttga ccatgttcgt
    20401 aaatttttcc ataacgcatg acttcttcta aagtagacgg aacgatttca ttttcaatca
    20461 ctaactcatg agagatttta ttgaaccatc tcgcttcttg atcagcatat tctgaaaaaa
    20521 gggtacttaa ttcttgcgaa gatgtaccca aaacatacaa ttgtgcttgg atcaatttca
    20581 tttcttctaa tttaaaattt gccaatacat gccccaccat ggcgccagca gttggtttat
    20641 gatgatcgat atctgcttgt tttgtttctt gagcaaattt ttcttccact atattcactg
    20701 atttacgcct cttttacttt tgttgattgg acttcataac ctaatttttc cacaactgca
    20761 gctaattgtt cagggtggac ttcatctgga tcaaagtttg ctttgatttt gctcgcatta
    20821 aacaaaactt tcgtattgct taccccaggc tgttgagaaa ccgcatgttc tattttttgc
    20881 atacagcttg gacaagctag tgtatctaat tgcataatca tttttttcat cgtagttctt
    20941 ccttcctttt tcttgatagt cttattctag attacagctt ccataaaaaa attgattctt
    21001 atcaagtttt acgatttttc ttctttgctt tcgtgtccaa aatacaataa acgcatcgcg
    21061 ttaaagataa cgatcaagat actagcttcg tgaacgaaca tcccactagc catgtaaata
    21121 aacccggcaa ataagccgat gagtaaaaat aagacgacac caatggcaat cactacattt
    21181 tcacgggtat tcaaaacagt tttcttagcc aaacggtaag catgaaccaa cgatccaaaa
    21241 ctagattgca tcaagacaac gtcactcgtt tcgatagcga catccgttcc tgatcccata
    21301 gcaatcccga tatcagccgc agccaaagag ggagaatcat tgattccatc tccaatgaaa
    21361 gcaacgtgca atccttgttc ttggtatttt ttcacaaatt gaaccttttc atctggcaat
    21421 aattcagcat ggacttcatc aatgcctaac atgtcagcta cataatcagc agtcatctga
    21481 ttatcacctg tcagcatcac taaatgtttt gctcctgctt gtttcaacat agctaattgt
    21541 tcagcgactt ctggtcgaat cacatctgac acaccaatga tttgagtgat ttttttgtcg
    21601 attgctacaa taatgactga gctgcctctt tgttgtaatt gatgtaaatc ttgtacttgc
    21661 tttggtgtga gagaaagttt gtgagcggca atcaatttgg tgttgcccgc acaaactgta
    21721 tgaccatcaa tctcagctgt gattccttgt cccttgatcg tctgattatc tttgactgct
    21781 aattgttgaa aatcaatgtc tttctcctga gcataatgga tcacggcgcg ccccagagga
    21841 tgatcggata acgtttctaa tcgagccact aaagctaagc tgtccccttc atcctctgta
    21901 tagttcttga tagtggatac agcagtagtt cctttggtca atgtaccggt tttatcaaag
    21961 acaaaagtat cgattttgga aaaagtatcc attgcttcac cgcctttgac taagatgccc
    22021 cgtttcgctc cattaccgat tcctgctaca ttcgaaacag gcgcaccaat gactaaagcg
    22081 ccaggacaac caagaaccaa aacagtgatg gctaaacgaa aatcacgact gatcagacca
    22141 acaagcaacg caataagtaa aacggttgga gtataatact gagaaaattg atcaatgaat
    22201 ttttccgcat gtgatttggt atcttgtgct tcttcgacta attctataat tttggcaaag
    22261 gtcgtatcat ctccgacctt agttgcttcg atcgtcacgt agccatctgc taaaatcgtt
    22321 ccagaaaata cgttatctcc actgattttg acgacttgtc gtgattcacc cgtcactgcg
    22381 gcttcatccg catacccttt cccgtcaatg actcgtccgt ccacaggaat agctccacca
    22441 gtttttacta agacgatatc gccttcttcc acgtcatcga tttccacttc ttcggtcgtt
    22501 ccatcttctt ggacaacgct ggcagtcgat ggggccatct cggtcaaagc cttgattgat
    22561 tcccgcgttt tggtcaatgt tttttgttcc agataactac caaacaaaaa caagaatgta
    22621 acgatagctg actccttgta ttctccgatc ataaaggcac caagcacagc aatagtaacg
    22681 agtaaatcaa ttgagataac tttgactttt actgcctgat acgcatggat tataataggt
    22741 atgaccgcaa taatagaagc tatggctaga gcaatgttat agaacaagac aaatccagta
    22801 aatttcccga taaaaccaaa aataatcaac acagctgata aggcggtgat atgatttcga
    22861 tgctcttgga tccacttttg taatttcatc tttctccctc ttttcctttt cattcttacg
    22921 agactaagta tagatactta tccatcataa aaaattgacc actatcaagt ttattcaaaa
    22981 aggcggtttt cttttggtat actgaattcc tacagtcatt aaacgatcat atagaaaaag
    23041 gaagcgccga aacgcttcct taggtagttc tattttttta ttcaaagata agatgctttg
    23101 tcatcttacg actgaagaac aacaatcaaa aaagggcagc agccactcat tcacaagaaa
    23161 tatcgttttt tgatatggga gttgaagcag tggacaaata tctctactta ttagctggga
    23221 ataagatcca aaaaagctta atggatttta tccaagaatt ggaatgtact ttccacaaaa
    23281 aattcactca ttccattctt ttaaaacttt tgattcatac agcgtgtcta attgagcgta
    23341 cattgataaa cggtcatgaa cttaaaatta tatcagaaga tgacacaaga ccttctcatg
    23401 aaacaatttt tcatgtaaaa aaggccttca aaaatattga aacagaattt gggatcactg
    23461 tttcttatga tgaatgtttc tttatctatg atattatcgc atctaaataa tcttttttta
    23521 tgtaaacgag ta
//
LOCUS       DAXWOO010000030        23373 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.030, whole genome shotgun sequence.
ACCESSION   DAXWOO010000030 DAXWOO010000000
VERSION     DAXWOO010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.030"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(22..2166)
                     /locus_tag="ACGE70_000929"
     CDS             complement(22..2166)
                     /locus_tag="ACGE70_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFF8692581.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(2523..3896)
                     /locus_tag="ACGE70_000930"
     CDS             complement(2523..3896)
                     /locus_tag="ACGE70_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFF8692582.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(3896..4291)
                     /gene="rbsD"
                     /locus_tag="ACGE70_000931"
     CDS             complement(3896..4291)
                     /gene="rbsD"
                     /locus_tag="ACGE70_000931"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFF8692583.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(4319..5236)
                     /gene="rbsK"
                     /locus_tag="ACGE70_000932"
     CDS             complement(4319..5236)
                     /gene="rbsK"
                     /locus_tag="ACGE70_000932"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFF8692584.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(5344..6357)
                     /locus_tag="ACGE70_000933"
     CDS             complement(5344..6357)
                     /locus_tag="ACGE70_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFF8692585.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(6438..7037)
                     /locus_tag="ACGE70_000934"
     CDS             complement(6438..7037)
                     /locus_tag="ACGE70_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFF8692586.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            7223..7924
                     /locus_tag="ACGE70_000935"
     CDS             7223..7924
                     /locus_tag="ACGE70_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8692587.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            7934..10645
                     /locus_tag="ACGE70_000936"
     CDS             7934..10645
                     /locus_tag="ACGE70_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFF8692588.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            11096..12724
                     /gene="treC"
                     /locus_tag="ACGE70_000937"
     CDS             11096..12724
                     /gene="treC"
                     /locus_tag="ACGE70_000937"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFF8692589.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(12784..13965)
                     /locus_tag="ACGE70_000938"
     CDS             complement(12784..13965)
                     /locus_tag="ACGE70_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFF8692590.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            14217..15740
                     /locus_tag="ACGE70_000939"
     CDS             14217..15740
                     /locus_tag="ACGE70_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFF8692591.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(15809..16536)
                     /locus_tag="ACGE70_000940"
                     /pseudo
     CDS             complement(15809..16536)
                     /locus_tag="ACGE70_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
     gene            complement(16549..17829)
                     /locus_tag="ACGE70_000941"
     CDS             complement(16549..17829)
                     /locus_tag="ACGE70_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFF8692592.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(17970..18572)
                     /locus_tag="ACGE70_000942"
     CDS             complement(17970..18572)
                     /locus_tag="ACGE70_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFF8692593.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            18817..19023
                     /locus_tag="ACGE70_000943"
     CDS             18817..19023
                     /locus_tag="ACGE70_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692594.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            19335..20054
                     /locus_tag="ACGE70_000944"
     CDS             19335..20054
                     /locus_tag="ACGE70_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFF8692595.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(20135..20818)
                     /locus_tag="ACGE70_000945"
     CDS             complement(20135..20818)
                     /locus_tag="ACGE70_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFF8692596.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            21036..23222
                     /locus_tag="ACGE70_000946"
     CDS             21036..23222
                     /locus_tag="ACGE70_000946"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFF8692597.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         7776 a         4211 c         4583 g         6803 t
ORIGIN      
        1 acatcccctt tttaatttat tctatttctt taaaggcaga tcaagccaga atgatttgtc
       61 taaacttaac aaggttagta caggaacagt tgcagcttta actgtacgag tagttgtttc
      121 tggtatctgt cttgccgtaa aattgttcca caaatcccag attgaagcga cacggaagat
      181 ggaagaagcg ttgttgacgc gttttatcgt tgaaaattct aatcctgtga ttggcacgcc
      241 atctgttgtc catttgattg gcacatcgaa tcgcttagac atattgagga tttcagcgtt
      301 tgcttcttca gttgttgctg cttctttttt cggttcatca tattttgtca ggtcgtacgc
      361 ttcgatgatc gcgttcaatt tttgtgaata ttggcgatct gtagcataac gtccttccaa
      421 atatttggtt gcttctttgt agtcttttgt ttccgacttc caagttggtt tgtagaaatc
      481 aggattgcca gtcagtccat ttttgatcaa gtcggcatag tcttccaaac tttccttgta
      541 agaaggatat ttacggaatt gtgcgcggat ttggtagctt tgtccggaac tatcttgttc
      601 aagtgtattg aaggtcacgc tgcttccttt atatgatcct ttgataccaa aaaggttatg
      661 atttggttcg cttgataata agctgtttcc agatccactt tcaaggatag cttgagccat
      721 catgactgat gcgtagattc cttctttttg cccgatttcc tgtgcatcat ctgcaatctt
      781 tttcaagaac tgatcagttg tttggttttg actgaaatgg aacgagccat ctaatccatc
      841 taaaatagat gagccgcctg tcagcgaacc tgaagcagca ggtaaacctg ctaagtttac
      901 tcggacagca aaatcaggcg tgaaatctga aatagaagtt actcgaactt tatctcctgg
      961 ttgaggcgca tggataaatt gtccgttccc aatatagata cctacatggt gagtaacacc
     1021 ggaagaaggt ccccagaaga cgaggtcgcc aggttgggcc tgacttactg gaatctgcgt
     1081 acctgcatat tgctgttcgc ctgtccagcc accgatataa tgaccggttg ctttcatata
     1141 tacgtaataa gtcaaaccag aacagtcaaa agtatttgga ccttttgctc cccatacata
     1201 tggttttcca agatgcttga atgcttctcg aacgattgct tgttggacat tcgttgtatt
     1261 tgtaggcaat acagcatcaa ttggtgattt tacaacaaaa tcagaagaag cagaagatgg
     1321 attctctata ccgctttgat ttgctgtatt tggacgagat gaagcattgt tcgttccgcc
     1381 attcgatgaa ccattgtttg gatggtttgg ttctctctga gtatttccat tgttattcga
     1441 tccagatggg tttgtttgat tcgaagaatt cccgctgttc gaattattgt tattattgtt
     1501 tgaattattt gaaccattat tattgttgtt attacccaca tctggtttgt ttggcgtttc
     1561 cggatctggt gtactaggga cttcttcact aggatcagtt ggtttctctg ttgaagtatc
     1621 cggtccagct ttaaattctg cagtaataga taaattgttc gtacctactg taaaggtctt
     1681 tgcatcataa tttacttctt tggcaccatt aacagcgact gaagcaattt gttcaccttc
     1741 agctggcgta atagtgtaag ttaccttcgt ttccgctaat aaaccttcta ccgttccact
     1801 ttccgtaata tccaaaggtt gctgttcagg ggaatccgct gtcgaagcag tcagttggat
     1861 cttaccaatc aaagaaggag caattgtaag ggaatatgtc tcggctggct tttcattttc
     1921 tgatggatca gccggatcaa tttcagttgg ctcggtcgat cctgttgttg agttagttgt
     1981 tccagttggg tcttctgtaa aagaatccgt tgtttcacta gtagctgagc tggatgtagt
     2041 cgtatctgtt gtgactgttt gatcagctat ttgattatct acggcaaata cggtattggt
     2101 cgacaaaaca gacggagcaa tcagccaagt agcagctagc aaacgtatcg tttttttatt
     2161 tttcataata aactagtcct tttcctttca ttgattttct gtcttattca gcattcatta
     2221 gacatttttt ggaacagata atcgtgtcga aaagcttttt cttttcataa atctgtctgt
     2281 gtattctaaa taaaacaatt tgtaaataag caacgagcat tcattcgttt ttctttgacg
     2341 ttcattttat tgcatagacc acctttgttt ggggataaac aagtggggat aaaaatggtt
     2401 atgcatatgt aaattatacc aaagtttgga aagtatcaca aaaatttata aaaataatga
     2461 tcctaaaaag aaaaagactg aaatatgtga taaacatctc atacttcagt ctttttcatt
     2521 aactattcat aagacattag gtcttcatca aacccgctaa cgagagtagt gatcattgct
     2581 acgactagac taacagccag tcctgctaag taccagaaaa aagtaccgga aatgaatgca
     2641 ggcaatgtgg tcagactacc aaatacaaaa gcgatcgctt tcacttgggc aaatcccatt
     2701 acagctccac cagcagctgc accaatgcaa gcagcaacaa atggtttttt caatgggatc
     2761 gcaattccgt aaacagttgg ttcggtcact cccgcaagta aaatcgaagc aaaagcagaa
     2821 ccggagatcg aacgggtttt tgaacgttta cttcgcagaa atacacctaa agctgcacca
     2881 gccattccaa aattggctgc tcctgtcaac ggaacgattc ggtcgaaccc ataagcagag
     2941 atattactga tcatgacagg gtttacagcc cattggatac caaatattac caaaatattc
     3001 caaccaccag cgatgattgc gccactgatc ggtccgttaa tatccatcat ccaagtaatt
     3061 gcggcagcta aaccttcgcc aaggtaaaca cccgcaggcc cgaataccat taacgtcaaa
     3121 ggaaccgtga taactaatga aagtaaaggt acaaatacta attgcatatt ttcgcgaata
     3181 tattttttga ggaatttttc aacaaaagaa taaaagaaaa tacctaaaat cgccggcatg
     3241 acagtagacg tatactgcat cagtactaca ggaatcccca gaaaatcagt agctgctttg
     3301 ccactgtctg aaagtaaaga ggtcagattc ggttcaatca gtgctgcagc aatgacagcc
     3361 gcaatatatc cattcacttt aaacgttttg gcagcagaaa aagcaagaaa gattggcaga
     3421 aaataaaaga cggcattcga cgcagcagac aaaatatgat aagttccgct tgtttcacta
     3481 agccagccaa tcgtagtcgc taaaacaacc agacctttta atataccaga accggcaaac
     3541 agcggaatga tcggcgtaaa gatttccgat atcaaaccaa atagtttttc gatcggattt
     3601 agcttttctt tcggagctga atgatcgatt tgacgcgtca attccttgta agcaggagcc
     3661 aatccctgat ccatgattat ttctgcatat ctatcgagta aaatgaaata cttaatggaa
     3721 gggatttgct gttttaattt ttctgcattg gctactggct gttttttcaa tttgatacga
     3781 agccgagtcg cacatggaag gacagaatca atattgtctt ttcctcccaa ctgttcaatc
     3841 agcatatgaa tctgctcgga cagtaccatc tgttcaggat tcattgtcgt ttccattaga
     3901 agaccactcc actcactaat ataatattgg cgtaaggtgt catttcgcct gtacgaataa
     3961 atgcatgaca gttattcaac tcttttttca tttcttcatg tgggataaag ctaatcggtg
     4021 tatctggtaa caataatttg attttttcca atatttccgg gttcattgtc tttatttctt
     4081 ccgccaattc aatcgattgt acctcaagtt cttctaaaac gttttctaaa acagataaaa
     4141 aacttggaag accgtttgag acagccaaat ctattttttc agtaccaaag gggacaggca
     4201 tgcctgcatc cccgatactt aatttatcaa agtgccccat ttgtgcgatt actcgtgaaa
     4261 tatctgaatt gattactttt ccttttttca tgtttgtttc ttcctctcat tgttaatttt
     4321 agactgctga ttgttctaaa gcttcgcggt aagggataga aggttgcgca ccataagact
     4381 gcacagcaac agaagaggct agatttccat aaaggatcgc ttctttcaag ttactaaggt
     4441 ctggctgaag tgtgctgctt aatgcgccaa taaatgtatc acctgcggct gttgtgtcaa
     4501 cggcatccac tttaaaagca gggacgatac cgtgttcttt ttctgtatga tagaaagcgc
     4561 cggcactgcc tagtgtaatg attactgttc caatacctaa ttcatggagt ttttcggctg
     4621 ctgcgaccaa actgttatga tctgtcactc tgattcctgt aatgatttct gtttctgttt
     4681 catttggtat aatgatatct gtcttttcaa gtagttcagt aggaatcgtt tcacgagctg
     4741 gtgcaggatt caggatcgtt gtttttcctg cgtcttttgc aatagaaaaa gctaggatcg
     4801 tcgcatctaa gctactttca aactgagcaa taataaagtc actgtttgta atcagttgct
     4861 tgttttttag gacttcttgt tcatggaaag cattatttgc ccctgcatgg atcaagatac
     4921 tattttctcc tgctgcgtct accataatca ttgcttgtcc ggttgtggcg ccttggattt
     4981 cagctatccc agatgtatcg atgttttctt ttgtcagtag atctaataac tgttgtcctt
     5041 cgctgtcagc cccaacgcct ccaataaaag atgtccgtgc gcctgaacgt ttggctgcca
     5101 ctgcttggtt ggcaccttta cctccgccag cgtgaaaaat ctcatgcgca tgcatcgttt
     5161 cgcctggctt gggcatgttg gttaaacgaa gggtagtgtc catattaata cttccaatta
     5221 ctgtaaccgt gttcatttta caaacctcca actatctcat ttattttgat gggaaaatta
     5281 ttttttaaaa cgttttagta aaacgtttta atagttcgat aaaaatatag cacaccttga
     5341 aagctatttc aagggtgcag ttgaaaatct tttttctaat cgtacaggta atcttttttc
     5401 ttcccattct ttttctggga attggatccg atctaacaat aacttggcag atgtttgtcc
     5461 caattcgaag attggttgtg cgatcgttgt cagtttcggc ttgatatact cgcacatatc
     5521 tatattatca tagccaataa tgctgtagtc ttcagggatg ctttttccag cttcctctaa
     5581 tccgcgatat aatccaaaag ctaattcatc atttaaagca aacacaccag ttgcagcaga
     5641 atcaagaagt tctgctgtga tctggtagcc accttgtttt gagaactgtg taggtaagag
     5701 gatcagttgg tcgtgaggga tttggtaaac atcaagagca gatttaaacc cctcaatccg
     5761 agcgtggaca ttttccggtg gattttcagg atagacaagg gcgatcgttt gatgtcctag
     5821 actaagcaag tgcatgcccg ctaggtaacc accgcgaaaa tcgtctgttc gtacggcatc
     5881 actaaatcct tcagattttt tctgatccaa aacaatgaag ggccggcctt gttttttcaa
     5941 attttcgttg attgcatctg tagagacagc agaagtcgcg ataatgaatc catccacacc
     6001 tcgacgtgtg agctcggcta gatattcgat ttctttttga tgatcagaat cagcattaca
     6061 taatatcgtg acgaaattct gcttatacaa gatatcctca attcctcgca tcagtgtact
     6121 gaagaatgga ttcgtgatat caggaacgag tacaccaatt gttttcgttt ccttggtaat
     6181 catttgtcta gcaaaatagt caggctgata gcctaattct tctttggcag ctaaaacttt
     6241 ctgaacagtt ttcggactga atttctccgt atttccattt aatatcaagg agacagttgt
     6301 gattgaaacg ccggaaagtt tagcgacttc tttgattgtg atcttttttt tcaccatgtt
     6361 catactccta tctaaatcct gtggttattt tcccatgaaa tcaaaaagaa gtaaatgaaa
     6421 atcacgtgtg ccagtactta tttgctttgt tggatagctt cgattatttg tttcgttgag
     6481 cgtactcgtg cagttttcgg aaatatcttt tttatgaaca attgatggga ttctttgtca
     6541 cgatcactgc aagcatcttc agcaagcacg atatggtacc cgtgttgaaa ggcatctaat
     6601 gcagtagcat atacaccgtt tgttgtcgct acaccgctta aaatgatagt ttcgattcca
     6661 cgccggcgaa gttgaagatc aagagaagta ccaaaaaaag cacttggatt gtattttgtg
     6721 atagtcaaca tattatctgt atctttcaaa gaatccttca aaagtaaatc agtgaattct
     6781 ggaggtacag aagcagtcat tccacccata tcagtttgtt gactcaaata agggaaagtg
     6841 gagatgtcta cgttcactaa aactttcaag gcagcggtac gtttcaatgt tcttactaac
     6901 aaatcattgt ttgtcagaat agatgctgca gtgtgtggag agagtgtacc gttatttacg
     6961 atctcttttt gcatgtcgat cgcgacaaaa gcagtttttg ataaatcaaa aatcatattt
     7021 tcctttaaat cattcatgat gttccttctt tcaaataaca atgtttttta tgtaagtaaa
     7081 tcataaatcg tttatttgtc aactaaatta aaggattcct ttctttagtc tagagaaatt
     7141 cagtggtaaa ataaataagt gtgagaaaac gcattcgatg catagatata gcgagaaata
     7201 tcgtgaaaat ggaggaatca gagtgaagta tgtcgaagta aaaaatgaat acaaacgata
     7261 caaaatggga gaaacgacca ttacagcaaa tgacgatatt tctttttcaa ttgaaaaagg
     7321 cgaattagtg attatacttg gtccaagtgg agcaggaaag tcaacgattc ttaatatttt
     7381 aggtgggatg gatacacctg atgaaggtca gatcattatt gatgacacag atattgcgca
     7441 attttcagat aaacagctta ctgcttaccg acgaacagat gtagggttcg ttttccaatt
     7501 ctacaatctc gttcctaatc taacagcaaa agaaaatgtg gaattggcaa cggaagtttc
     7561 tcctgatgcg cttgatcctg tagaggtatt gaggcaagtg ggcctagctc atcgattgaa
     7621 taactttccg tcccaattat caggaggcga acagcagagg gtttcaattg ctcgtgcact
     7681 agctaaaaat cccaaacttc ttttatgtga tgaaccgaca ggcgcgctgg attttgaaac
     7741 gggcaagcaa gtattgaaat tattgcaaaa cgctagcaga caacacggca ataccgtttt
     7801 gatcattaca cataattctg ctttagcgcc catcgctgat agagtcattc acattaatga
     7861 tgctaaagta aggtctgttg aattaaatga tcatccttca tccattgatg agattgtatg
     7921 gtagggggat tttatgaaaa caagaacata tcttaaagca ggttttcgcg aaattcggca
     7981 atctaaagga cgattcattg caatcatcct tattgtcatg ctaggaacac ttttatttgt
     8041 aggtgtcaaa actgctggac ctgtcatgca aaaaacgatg gatcactatg ttggtacagc
     8101 aggactttct gatttgcaaa tcgtttctac tggtggcttg acacaaaaag atattgctca
     8161 agcagaaaag atcccagatg caaaagtaga aacaggaaaa cagatctatt attccaatac
     8221 tggcaaaaac gaagtgattc aaatattttc ttataacaaa aataacaagc agaacaagct
     8281 acaattaact gatggaaaac tcccggaaaa accaaaccag cttgtattgg atgaaaaagc
     8341 ggaagatgaa gggtataaaa tcggtgatat ttatcagatt gattcagatg aattgaaaga
     8401 aaaagagtat accatcacgg gctttgtccg ttctccattg ttcatcaaca atttggagag
     8461 aggatacgcg aacgtcggaa atggtagcgt cgattatttt gtctatttac ctgaatccgt
     8521 ttttaaaaca gatatttatt ctgtcttata tcttgatttt acaaacgtaa agaatttgaa
     8581 tacgtacagc aaagcttaca aagataaaat ggagcaaaat caagaaaagg cagaaaagta
     8641 tttacaagat cgacccgaag aacgcttaga agagctgaaa aaatcggcca acgaagaatt
     8701 agaaccggca cagcaaaaaa tagcggatgg caaagcacaa acaacgcaag caagaacacg
     8761 gttagaagat gctaaaaaac agttagcgca acagtcagca actatccagc agcttcctga
     8821 agcgcaacga acaatcgctg ttcaaacatt atctcaacaa gaagctcaac ttgctgaaca
     8881 agaaaggcaa ttagttgaga aagaacgtga actggcctct gcagaggaag aattgaatcg
     8941 aaatgcagaa aaaattgaaa atttaactaa accatcatac ctatacaatg aacgttcaga
     9001 aaatgtcggt tttcaagaat ttggtgatct gtctgatcgt atcgctgcga ttgccaatgt
     9061 ttttcctgtt ttctttttct ttattgccgc attgatcacc tttacgacaa tgacacgtat
     9121 ggtagaagaa aaccgcaggg aaatcgggac attaaaggcg ttaggttata cgaaagttga
     9181 aatcgcaaaa aaatatgcaa tctatgcaag tctagcctca gggataggta ttgttttagg
     9241 aacgatatta ggaacgaatc ttttgccaag gatcattttt gagctgtcta acgagcggta
     9301 tgatattggg agtgcgctta ttttttatga ctggccgccg attttacaag cagctgccgc
     9361 attcttcatt gccgcatttg gcgctgcaat gatcgtttta ttcaaagatt taagagaacg
     9421 gcctgctgca ttgcttcaac cgaaagcacc gaagccaggg aaacgcattt ttcttgaata
     9481 catcactccg ctgtggtcac gccttagctt taatcaaaaa atcagttacc gaaatctctt
     9541 ccgatataag tcaagaatgc ttatggcgat tatcggaatt gctggatgtg ccgggctgat
     9601 ggttgcaggc gtgggattga aagattctct aagttctgtt tcagccaaac aatttggacc
     9661 gatcattgac tatcaagcaa tcgttacatt ggattcatcc acggaagata gtcgtcaaaa
     9721 agcagcggat atgctgcaga agcaagaaaa agtaaatgag atcctagcta ttcatactca
     9781 aaccgtagaa ttgagaaaaa aaggacatgc ttcgcaaagt ctgacagtga tcgtaccaag
     9841 taaaacagct gatttggatc gttttatcca tttaactgat cttgaagaga acccacgctc
     9901 cttgcctaaa acaggaatcg ctattacgca aaaagtagca gaactatttg acttatctac
     9961 aggagatcat atttcgcttt atgatgaaga tcaaaaagaa tggaaagtcg aagtaaaaga
    10021 aatcgtgaaa aactatctag gtagctttat ttatatgtcc cctgaatatt atgcttcagt
    10081 tactgatcaa tcgatgatgg ataatgcatt tttgctgcgt acagataaga tgacaacaaa
    10141 agaagaagaa atgctttccg agaaactgct tgccacagac gctgtgacaa atacgtcttt
    10201 catgtccaaa caaatcgaaa cgcaagaaga atcaatggcg aacttagact cggttgtctt
    10261 aatctttgtc gtattatcag gactgctggc ttttatcgtc ttgtataatc taactaatat
    10321 caatatttcc gagcgtgtac gcgaattatc tacaataaaa gtccttggat tttttgataa
    10381 agaagtcacg atgtacattg ttcgagaaaa tatcatcttt actcttttag gaatcatcgg
    10441 aggatttggt gtaggatata tattgactga cttcatcttg caacaagcgt ctatggagaa
    10501 tgtcatcttt ccattagtga tcacatggtg ggcttatgtc ttatcagcag gattaactat
    10561 cgtatttacg gttattgtga tgattgttac ccatttcaaa ttaaagcata tcgatatgat
    10621 tgatgcgttg aaatcgaatg aataaaaaca gaggttgtga aatcagcggt ttgacctgag
    10681 tattagagaa acaaacggca aaatagtttt tcatattttg ccgttttttg agctaatacc
    10741 gaaggacagc tgattgaaca ccatgaatca gaggttgtaa aaaagccgtc ctgcatcgag
    10801 caaaaagaat actcaaataa aattagctcc tcatgtttta catttgagaa gactgattgc
    10861 cgtagataaa agcttttgaa caccatttat agaagaaaca agaatatttt gctattttaa
    10921 acaagaggat agacaaatac atgtctgatc tcttgttttt tattgggcaa cagacctctt
    10981 agaatgtata aagataaatt ttacaaacgc tttcggttca gttataataa taaatgataa
    11041 cttgtatata taagttgtgg taggtaataa atcagttgaa tggaggtaaa tatccatgac
    11101 atttaaagac aaagtcattt accaaatcta tcccaaatct tacaaagaca gtaatggtga
    11161 tggaattgga gatttaaaag gaatcaaaga aaaactcccg tatctgaatg atctaggcat
    11221 cgacatgatt tggctaaacc ctatttatcc aagccctcaa aaagacaacg gttatgatat
    11281 ctctgattac acagccatcg atcctatctt tggtacgatg gaggaattcg aacagctaat
    11341 agccgaagca aagaaatacc ggatagaaat catgctcgat atggtgctga atcacgtttc
    11401 gacagaacat gaatggttca aaaaagcact agcaggggac cagcattatc aggacttctt
    11461 cattttaaga gaagagccaa cggattgggt atcaaaattt ggtggaagtg cttggtcgcc
    11521 atttggagat acggggaaat attatttgca cttatatgat cggacacaag ctgacttgaa
    11581 ctggcggaac caagcagtaa gagaggaact attcaaagtt gttcgttttt ggatggaaaa
    11641 aggggtaaaa ggattccgtt tcgatgttat caatgttatc ggtaaagacg aacaattgaa
    11701 aaataataaa gaaaatgatg gcaaacctga atatacggac aaaccaatca cccatacgta
    11761 tctaaaagaa ttgaaccaag caacgtttgg gacggattcc actatcatca cagtcggtga
    11821 aatgagctct acgacgatcg aaaattgtat cttgtataca gaacctagta gagaagaatt
    11881 atcaatggtc tttaatttcc atcatttgaa agtcgattat gaaaatggga ataaatggtc
    11941 tactcctcca tttgattttg cagaattaaa gcgactattt catacatggg gagaaaagat
    12001 gagtgagggt aacggctgga atgctctatt ttttaataat catgatcagc cacgtgcgct
    12061 caatcgattt gtagctatcg atcagtatcg aaaacaagga gcagagatgc tagcaaccat
    12121 gatccatttg aaccgtggga cgccctatat ttatatggga gaagaaatcg gcatgatcga
    12181 cccagactat tcatcgattt ccgactatat tgacgtagaa agtttgaatg cttataagtt
    12241 actccttgaa caaggattca cagaaaaaga agcgtttcgt cggatcaaag caaaatctcg
    12301 cgataactct cgaacaccca tgcaatggtc agacacagaa caagcaggat ttactacagg
    12361 gaaaccttgg ttaaaagttg gaggcaagct agaagagatt aatgtagaaa aagaactatc
    12421 ttctgaagat tctattttgt cctattacaa gaaattgatt cggctgagaa aaacatatcc
    12481 aatcgttgca caaggagatt atcatgctta tgaagcaaat catccggaag tttatggctt
    12541 tcttcgccaa tttgaaggac agcagctgct tgtattaaca aatttctatg ataaagatac
    12601 aaggatcgcc atcccagaag gattcacaga agccaatatt cttatcgata attattccga
    12661 tactgttctt catcaaagtc tggagctaaa accataccag gcaattgctt tgctgaaaaa
    12721 ttaaaacaaa aacacaaaac gtcgttccta cgagctattc tcgtaagatc gacgttttgt
    12781 gttttattta aaataatctt cttcagaaaa tgaagagttt tcatactctc ctttatcgcc
    12841 ataaaattta ttgtagcgtt gtttgaattt acggaaaata aaagtaacga gtaatttgac
    12901 taacgtatag ataggaatac caaaaataac gcccacaatt cccattagat cacccatgac
    12961 aagtaaaacg attaagatcg taatcggatg gatctgtaaa cgatcaccca ttactcgagg
    13021 aatcaccaag tcaccgtgga gaagttgtac ggcaaaccag acaatgacaa atttgacagc
    13081 cataaaagta gagtcttgga atgcaatgat caaacctgga aagaaggcga taaacggccc
    13141 aatatatgga atgatacata acaacccagc agaaatagct aaaacactag caaattctaa
    13201 tccgatcagt aagaaacaaa cccagtagac tgctccaagg ataaatgaag caatgatttg
    13261 cccctttaga aatgaaccca attgttgatt agcaatcttt gtcaaatttt taaaatctgt
    13321 acgaaatgct ggtgggacaa tcgctaaaac tgactgataa aatttttgag gatctctcaa
    13381 aaggaaaaaa gcgacaattg gaccagtaaa taaagtaata aaaatagctg taacagttga
    13441 aaaaatgttg ctgatccgtt gtgcgccact ttcccaataa tttccaataa atccagctat
    13501 atcagacgtc acgtcgtcaa tagaagcgaa aaattgatta aatgaatcgg caaaaggtgt
    13561 tttctccaaa aattgtcgga gactctcttg aaaatcttga aacaaatctg gcaaactact
    13621 gatcaatgtc tttgcctgtt cttcaagaaa aggaataagc catatgcttc caagccccaa
    13681 taaaaataaa ataatcacat aaacgataat gactgacaag ttcctcgaaa ggtgcttgct
    13741 caaacgtttg atgactggtt ccaaaagata atacaagatc agtgcaaaca ataatggggg
    13801 caagatagta gcgataatga ttccgacagg cttgaatata aacgagatct gatggaaaaa
    13861 ataaatgatc agcgcaagta agataactat tcctaaaaca tagtaagagg tttttcctcc
    13921 taaaaagcgg atcaaacgat tcgttgtatc tgtttctttt ttcactcttt ttcctccttt
    13981 atctataact tcattataga caaaagcggg tataaaagga aaatgataga ggttgattgc
    14041 aaaaatatcg tcaatcattc atataaactt tctcatcaat taaaataaga aaacgcttga
    14101 cagttctttt aaccatatga taaattaaat cctgttgcta aaacgtaact attacggttt
    14161 gcgaaaagcg aatcggaaca ttcaaatgaa agaagatgag aagaggagtg tcataaatga
    14221 aacgattgat aggtatattg gccatgctgg ttttagcagg tatgttaact gcttgcgggg
    14281 caagtgggaa agccgaggac agtaaagaaa aattgagcgt gatgacgacc ttttatccca
    14341 tgtatgattt tacaaaagca atcgtcgggg atgaaggaga agtagagtta ttgattccag
    14401 cagggactga ttcccacgat tatgaacctt ctgctaaaga catggcaaag atccaagata
    14461 cagatatctt tgtttataat gatgaaaaca tggaaacatg ggtccccgca atccaaaaaa
    14521 cattacaaga aggcaatgtc catacgataa aagcgacaga aggtatgctt ttacttccgg
    14581 gaagtgaaga aggacatgat catgaccatg aacacgggga agaaggccat acacatgagc
    14641 tagacccaca tgtatggcta gctccaagtt tagcaatcaa gcaggtagca aacatacgtg
    14701 atcagctgat tgaagcttac ccagaaaaac aggaagtatg gacaaaaaat gcagcagcct
    14761 ataccgagaa acttcaagca ctccatcagc tttatcaaga aacctttaag caagcaaaac
    14821 aaagatcttt tgtcacgcaa catgctgcat tcaactatct agcgttagaa tatggattga
    14881 atcaagtatc gattgctggt ctttcttcta gtgaagaacc gtctgcggcg agaattgctg
    14941 aactgaaaca ctttgttaaa gaacatggaa tcaactatat ttattttgaa gaaaacgcca
    15001 aagatagtat tgccagaacg ttagcaaacg aagcaggtgt atctttagaa gtattgaatc
    15061 ctttggaagg attgacgaat gaacaaatag aaaatggaga aaactatctt tctatcatgg
    15121 aagcaaacct cgaagcgttg aaaaaaacga cagagacaga aaacccgcta gaagatactt
    15181 tgacccccaa aaaagaaaag agcgtctaca acggttattt tgaagacgag gatgtacaag
    15241 atcgctctct ttcagattgg gcaggaactt ggcaaacagt agatacgtat gtagaagatg
    15301 ggacttttga tcctgttttt gaatataaag aaaaattgaa tcaggataag acagcacaag
    15361 aatataaaga atattatacg aagggctatc aaacagatat tgcttcaatc aagatcagtg
    15421 agaaagagat ggcatttacg tttaagaatg gaacaacgaa acgttcaaat tatcgttatg
    15481 tagggaaaga aatcttgacc tacgaggcag gaaaccgtgg cgtaaggtat ctattcgaag
    15541 cagaagataa ggaaagtggc gcttttagat atattcaatt cagtgatcac acgattgcac
    15601 cggctgactc cgaacatttt catttatatg caggaaatga aagtcaagaa gtattgctga
    15661 aagaaatgga gaattggcca acattttatc cagatacaat ggatggacgt gaaattgcac
    15721 aggaaatgct tttgcactga taggcaaaaa agaaccgaag ggtttggatc ggctggtcat
    15781 ttagtcggtc caaactattc ggttcttttt atgaaatcaa tttctttaat ccacggatag
    15841 aggctgggat ggtctcgccg tttgccaacg tgacttctct ggttttttat taatcgaccg
    15901 cacattttgt gtattgataa cataggattt atgacaacgg ataaaattcc gatgcatttt
    15961 ctcgatttct ttgatcttct catagaattg taattgggca tgagtcaaat ggacttccag
    16021 tttatgtggc gctcctagcg aagtagaaaa atattggatg tctttgatcg gtaaaaattt
    16081 gatctcatca ttaaactcta ttttgaagat atctttttgg atttgggaca tttgaaaatc
    16141 ttcgaaaatt ttgctcagga tatgctcgac attttctttt aaatcataaa gatcttcttt
    16201 gacgatataa tcagtcggtt cgatgttgct ttttaagatt ttcatcgaca tttccgtatg
    16261 ggaagtcaca tagattattt ttcctaaagg atctaattca cggatcaatt taccgatcgc
    16321 tacaccatcc atttcactgt cttctagttc gatatctaaa aagtaaatac caaaagaaga
    16381 agttctgatc ttggctgcat tgagaagttc ttccggatca gaggtagaaa tatggacata
    16441 actgtctaag ttttcaatca tgattctatt tttgatcacc gttgataaaa cttctaattg
    16501 ttttggatcg tcttcacaaa tataaatggg aatcatatca ttactccttt attttatttg
    16561 tatataaatc gtttgagaaa aacgctggtt ttcaattgat gtttcaagga aaagataagg
    16621 attggtcttt gtcaattcat ccaagataaa caagccaagg ccttcgtttt ttggatttgt
    16681 ttttgttgag aacccttttt gtttcaactg gtttaacggt aatgtatttg ttgttgtatt
    16741 gatcacacga atcatcacgg aatgattctt cttgagtatg gtcaattcga tttttttctt
    16801 ctttgcagca ctagctcctt caatcgcatt gtccaagata ataccaagca tacgtacaag
    16861 gtttaccgta tgtttcgtat ccagttcgat tggctcagga aaatgaagct ggacgatcaa
    16921 ccctttttcc tgtgccatca tgagttttaa tgacagtaaa ctacggatct caggaacttt
    16981 catattatcc aacatactta gattggccgg aaaaaggttc atcatctggc gtgtaggtaa
    17041 tatcgtttga tcataataag accgtaatcc ttctaagtcg ccttctgaaa tatagctgtg
    17101 aagagaaaaa agcagatttt ggtaatcatg tttgaattct ctcaagtctt gatagttttt
    17161 ttctagatct ttgatataat cctgttgcat cctgacttgt tgctctaata actgatcagc
    17221 taatttctta ttagagattt tccaataata aaccaagatg atcaggccaa cacacagata
    17281 tagaatgaaa aaactcgtat tcaaagctaa gacatcaggt gtttcaccag aaaaacgggt
    17341 ataaaaaata atgatatagt atgtaaataa tatcaatgtc ccgatcgtac cgatcatacg
    17401 gcggtccaca gtgtgaaacc aagtctctaa cgattttttt aaacaaaaag ctaataaagc
    17461 agataaaaaa gcggataaga caatgtgaaa atactgcaat gaatctcctg gttcaataac
    17521 cgaagatttc aaaacaaaaa aatcgccgat gcttgttaag tgatctccta aaagcgacgt
    17581 caataaccca aaaggaagcg cgtaaaccaa ttgttgacgc gaagtcgaaa agaagaaaaa
    17641 agtaagcatc agcattccta tagcaattag agcagtaaaa atccctaaaa tcgaatagac
    17701 ccatccaatc aaaatacaag aaatcagaat gactcccgca aacagatatt tgtatcggat
    17761 gtgatggtca atcaataaga tacaaattgt gtaaatcaaa tattgcaacg ctatggacca
    17821 taaaaacata aagtacagct cccattgatt tttaccaaat tatagcacaa ttggcgtatt
    17881 atttttttaa tttttcaggg attttgaatt cagaactcat accaaagcag cagccagata
    17941 caaaaaagtc agataagtta gttaattgtt taatgattgt ttttttcatg aaagattcct
    18001 ccaaaattca tcaagtagtt tgtaatagga agcaacgtag ctatttctaa aacaatccca
    18061 agtagcaaat aggtagaaaa gtaagaagct gagaaaaata acgtaatgag agaaaaaatt
    18121 agaaacatgc tgatcgcctt ctttaataat gggggacgtt tctcgggttc aatgggattg
    18181 attgctgtac ctcttgatcc atagcgaagc agtaacaaaa agcagctgat taacaaaaag
    18241 accatcattg ggaaggagat agtcaattgc agatgtttca tcaccaaggg aacgccccat
    18301 aaataaagta gtgtccacag cagacaaacg aattcagagt cggaatgcaa tccataagca
    18361 tatctgcgga tgcagatata agaaagaaag gtgattcctg tttccgccca tgagcctgtc
    18421 accagagaaa agactagaac gaccgctagt tttccaagat tattcaataa aacttctaag
    18481 acaaatttta cttggacata caccatatct tcttccgatt tgtctccgct gaataaggct
    18541 tctaataatt tgttcacaac tttatctttc atctcatacg tacctccacc acaaatttta
    18601 tgctaaaaaa aataaaaaac gtcttttgtt tcataaaaag tggttttgac atcaaaaccg
    18661 tcatgatctc ctaaaaaaat cttcgttttc caagaacctt accaaaaaaa gtgacagttt
    18721 tgatacgaaa agtacttttt atgacaagtt tgtgcttttt cgagtgattt atgttaaaaa
    18781 attactgacc ttataaaagg tgatggaggg attcttatgg aaaatgaaca attgtttgaa
    18841 gtcggcgaaa catatgcatt taaatctgat ttatttgaat caccaataaa aggaaaaata
    18901 gaaaaaatct atgaaaatag tgtgatgata cgtgtcatga aatgcgcagt agaagacaat
    18961 gataccgctc ataatctgca ctggcagtta gttgtacgca aatcaagtac aatagaagaa
    19021 taaaaacaga taaaatgttg agagcataaa gaggttgtga gtctcccgtt tagctctgag
    19081 ttacaaggaa taatgttaaa aaacagcttt tcatgtttta tgaatattgc cttgtgaccg
    19141 aggtgttggc aggagaatac tgtttatttg aaaaaagggc tgcgacaaga cttttgtcac
    19201 agcctttatt ttcatgaata ggagttactt ttggctactt ttagagaagt agcttttaag
    19261 gagcagataa agtcaaggct tttatttcta taaatgaagt agtaaaatga agaacagatt
    19321 ttattacagg aaggatgccg ataattgtga tcattgttgc aggaacgatc ggtgcaggga
    19381 aaagcacact aactgaaatg cttgcccaag atttagaaac aaaacctttt tatgagaacg
    19441 tggaagataa tgaagtattg ccgctatttt attctaatcc agaaaagtat acttttttat
    19501 tacagatttt ctttttgaac aaacgctttt tggcaatcaa agatgctttc agccatgatg
    19561 acaacgtact agaccgctcc atctacgaag atagtatgct gtttcatttg aatgcagatt
    19621 tagggcgtgt ttctgaagta gaagtaaagc agtatgaagg tcttttagaa acgatgctga
    19681 aggagttaga agaaatttcg ccacaaaaga aaccagatct actggtttat attcgtgtat
    19741 cctttgaaac tatgttagcg agaatcaaaa aaagaggccg ggaatatgaa cagctagaac
    19801 aagatcctga actctattcc tactataaag aactcaatcg acgatacgaa gagtggtatg
    19861 aacagtttga tatatgtccc aaaatagtga tcgatgggga caaatatgat ttcgtagccg
    19921 atccggcatg tggagaacaa atcgtacaag aaatcaagat gcgggcaaag aaaatgacag
    19981 aagaggcaga tgcccagtcg atccgctaca ctacaaataa cccgaaagca aagggaatct
    20041 ttacaactaa ataatataaa aactaagcaa attgctcact cgcagatatt tctctgtgag
    20101 cagcatcttg cttagttttt ttgattcatt gagatcaaat agtgaaccga ttcgtatgat
    20161 aaaattgcca taaatgaaat gtctgagtga gggccatgat caaaaataaa atggaaacga
    20221 tcaaaatcga atgggcgcca aacagatgat gacagaaaac aaggacaata gctcctgcga
    20281 taaaggcaag aacgacactt agatagtgga aaaatcgatc aaaatctgct ttttctttag
    20341 aaatacaata atcatataag ttcgttgcta ccccttttaa atttccagtt gtaaataaat
    20401 tagtgtaggc acgcccgttg attttatcaa aagctaccca ctggatcgat gcagtaaaag
    20461 caatcaaaat cgttactaat gcgggatgct catggacggg tgtcaaacta acaaagaaaa
    20521 aaatcactgc ttcataatat agaacaaaca agcgccagaa atgtttttct ttttttctaa
    20581 aataatcaat tagatacttt gttagaagaa tccctaagaa aaagaaaagg gtagacaata
    20641 cttttttacc aacacttagc cattctccgt caaatgcttg gatgatcgat agaatgatat
    20701 tgccagtttg agcagaggca aaagcaccat attggatata agtataactg tccatggctc
    20761 ctccgataaa tgtcagcaaa agtcctattg tacgcccctc gtgaaaggaa gtctgcattt
    20821 ttttcatcac cttttatgaa tatttttgtc ttccttatct tagcataaac gattgaaaaa
    20881 aaggaaaaat tttcagaaaa ctaaaatcat atcgcttccc attttcattt ttagaaaggt
    20941 ctgttagtat aaagaaaaaa tagacaacga aactttaaat aaccaacttg aaataaacaa
    21001 attcaaagaa gaaagatggg aaggaaatca atcagttgaa tgaaaaagaa attctccgat
    21061 tacaaaaaga attgaaacgc tgggagaaca agtataaaaa gcttcaacaa gtctttcggc
    21121 tacaagaaga aatcattgct acctatgaag cggcttatgg cgaagcaccc ggacttattg
    21181 aggaagaagt acaagtaaaa gaaagtgaag gaaacagctc tttctttaaa atgatggaga
    21241 aacgttccta taatcgcttc aatcaagaac aaaaagcagc tatcacatat gatatgaaga
    21301 aacatcttcg aatcatagca ggagctggga gcggcaaaac gcaaacaatc tgtgcaaaag
    21361 ctgtttatct gatgacgcaa aaacaagtag acgaagaacg catactaatg atcactttta
    21421 caaggaacgc tgcaaatgaa ctaaaaaaac gagtagataa tttcagccag agaaaaaccg
    21481 ctgtccatat cggaacattc catagtatct tttttaggat atacaatgaa atttgtcgaa
    21541 aatttcctga agtagcaatg caaggaatac aaggagactt ttcaaaggat actgcacaaa
    21601 aagtgaatgc tgttttgcaa cagctgatac gtaaatataa tctttatata tttgatcaat
    21661 atggagaaaa aacgattgct tctcggttag actattggca gaatatgaac ctttcagtag
    21721 aagaaatggt tcagttagtt aaagaaaaat atgattccat tgataagaat gcacgacagc
    21781 cgatatcaga acgactgtat ggattgctca cggaactcca agaacaaaaa agaagccagc
    21841 aattgctaga gttcaatgat gtactccaaa atctgaaaag cgctttggag aatgaggaga
    21901 tccagcatta tatcggccaa aaatatgatt atctattcat tgatgaattt caagatacta
    21961 atccacttca atgggaaatc gtgaaacgga tgacaaagga aaacaggatc aagttgattg
    22021 ttgttggaga tgatgatcag agtatctatg gattcagagg atcagagcca gcatacatca
    22081 agaactttga aaaagaatat cctaccaaaa ctcttttctt attaactaat tatcgttctc
    22141 gggcagctat tgtccaagga gcaaatagat tgatcagcta taataaaaat gatcggatac
    22201 caaaggcgat gattccagca caaaaagaag caggtgttat ggaagcctat ttgttttctg
    22261 atactaaagc agaaagtcag tggctaatca aacaaattca gacatttatt caaaaaaatg
    22321 ggaaatataa ggaatcaatc atattatacc gatcagcttc acaaacgcag cagcttgtcc
    22381 aatctctgct gaaaacgaat attccttttg tactggaagc agatagtcca tatgaaggta
    22441 tctttggtat caagtctttc caacatttct atcaaaaaat tgttcattgg caaacagcac
    22501 tgaatctgca aaagaaaaat caggcctacc agcaattgct ccggcagctg atggcagact
    22561 gctacctgaa aaaaagtgaa tgcgatcaat acttttctcc taaatatcaa aatatagccg
    22621 ttgcagatta tattttgtcg ataagcccca atttaaaatc gaaatctgca gaaatccgag
    22681 agtttgagga agcgttgcgt aaaacagacc agcatcaaga aatctattcg cttgttcaaa
    22741 gctatcttca tctgccacgt atagctaagg aaatcgacca gagtgatcaa gaatggatac
    22801 gagaagaagt aaagcagcat gctactttca gaagactcca agccttatcg caagaaaccc
    22861 aagcgacagc taaagaatta aaaacccgct tgattgctta cagacaagga aaacttgatg
    22921 cactatgtat ccagagcatt cacaaaagta aaggtcttag ttaccgaaat gtctttttga
    22981 ttggttgtaa cgaaggaagt ttgccttata acggagcgac agaaaagaaa gtaatcgact
    23041 ttaatgaaat aaaagcagaa ccagtaacga cgatagaaga agaacgaaga ctattttatg
    23101 ttgcgatgac tagagcaaga aatcgtctgt atctatgtgt acctcaaatg aaaaattcaa
    23161 gaaaattgaa agtatcgaga tttatcaaag aaaccgggag tcgtgtgaaa aaaggaaagt
    23221 gatagaagag agggtgtgac agaagtggtc agtttcaaga aagaagaagg aatgtacgaa
    23281 aattgcttct caaatttttg tgaaatttca gcttattttc gaagaagcta tttctggaac
    23341 acagtttatt cggaaaaggg aggtgtgaca aga
//
LOCUS       DAXWOO010000031        23091 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.031, whole genome shotgun sequence.
ACCESSION   DAXWOO010000031 DAXWOO010000000
VERSION     DAXWOO010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.031"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(27..1523)
                     /gene="lysS"
                     /locus_tag="ACGE70_000947"
     CDS             complement(27..1523)
                     /gene="lysS"
                     /locus_tag="ACGE70_000947"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFF8692598.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="ACGE70_000948"
     CDS             complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="ACGE70_000948"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFF8692599.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="ACGE70_000949"
     CDS             complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="ACGE70_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFF8692600.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3743..5854)
                     /gene="ftsH"
                     /locus_tag="ACGE70_000950"
     CDS             complement(3743..5854)
                     /gene="ftsH"
                     /locus_tag="ACGE70_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFF8692601.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="ACGE70_000951"
     CDS             complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="ACGE70_000951"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFF8692602.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="ACGE70_000952"
     CDS             complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="ACGE70_000952"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFF8692603.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8054..8524)
                     /locus_tag="ACGE70_000953"
     CDS             complement(8054..8524)
                     /locus_tag="ACGE70_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFF8692604.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8570..8995)
                     /locus_tag="ACGE70_000954"
     CDS             complement(8570..8995)
                     /locus_tag="ACGE70_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFF8692605.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9062..9331)
                     /locus_tag="ACGE70_000955"
     CDS             complement(9062..9331)
                     /locus_tag="ACGE70_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFF8692606.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9342..10937)
                     /locus_tag="ACGE70_000956"
     CDS             complement(9342..10937)
                     /locus_tag="ACGE70_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFF8692607.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="ACGE70_000957"
     CDS             complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="ACGE70_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFF8692608.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14490..15050)
                     /gene="pth"
                     /locus_tag="ACGE70_000958"
     CDS             complement(14490..15050)
                     /gene="pth"
                     /locus_tag="ACGE70_000958"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFF8692609.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15403..16377
                     /locus_tag="ACGE70_000959"
     CDS             15403..16377
                     /locus_tag="ACGE70_000959"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFF8692610.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16547..17947
                     /locus_tag="ACGE70_000960"
     CDS             16547..17947
                     /locus_tag="ACGE70_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFF8692611.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18025..18747)
                     /locus_tag="ACGE70_000961"
     CDS             complement(18025..18747)
                     /locus_tag="ACGE70_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8692612.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18826..19332)
                     /locus_tag="ACGE70_000962"
     CDS             complement(18826..19332)
                     /locus_tag="ACGE70_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFF8692613.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19455..21254
                     /locus_tag="ACGE70_000963"
     CDS             19455..21254
                     /locus_tag="ACGE70_000963"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFF8692614.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21322..22896)
                     /locus_tag="ACGE70_000964"
     CDS             complement(21322..22896)
                     /locus_tag="ACGE70_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFF8692615.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLHQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
BASE COUNT         6322 a         5012 c         4222 g         7535 t
ORIGIN      
        1 gaattcctta gacagctttt attcgattat ctcattgttg ggaatagcaa aacatcccga
       61 attgattgtg catcagttag caacattact aatctgtcga tcccgatgcc tagaccacca
      121 gttggcggca taccgtattc taaggcttcc aagaagtctt cgtctacacc gtgagcttcg
      181 tcatttccta gttcacgttc tttttcttgc gcttcgaatc tttctctttg atcaattgga
      241 tcgttcagtt ctgtaaaggc attagcaaat tcgcgcccca ctatgaatag ttcgaatcga
      301 tctgtgaatc gaggatcttc tggatttttc ttcgctaaag gcgaaacttc gactggatgt
      361 ccgtatacaa acgtcggttg tgtcaatgtt tcttcaacaa atgtttcaaa gaattcattg
      421 ataacatgtc cgacacccat agcttcggtt acttctacat tgtgtttttc ggctaaagct
      481 aatgcttctt ccaatgtcat ttcttgccag aagtctacac cagtttgttc tttgattgca
      541 tctaccatat ggatgcgttt aaagtcactt tctaagtcga cagcttgtcc atcatatgtg
      601 atttttgctg ttcctagtac tttttcagct gcgttacgga taataccttc tgtcagattc
      661 attacatcat tgaaatcagt atatgctgtg tatgcttcta acatcgtaaa ttctggatta
      721 tgtgtagtat caattccttc attacggaaa acgcggccga tttcataaac tttttccatt
      781 ccgccaacaa ttagtcgttt taaatgaagt tctagcgcaa tacgcaaata taaatcaatg
      841 tccagtgcat tatgatgcgt gataaatggg cgtgcggcag ctccacctgc ctcattgtgc
      901 aatactggtg tttctacttc aatatagcca ttgccatcta aatagcgacg gatttcgctg
      961 ataatttggc ttcgtttcat aaatcggtca aagctttcgc gattactgat taaatccaag
     1021 tagcgttgtc tgtagcgttg ttcaatattc gttaagccat ggtatttatc tggcagtgga
     1081 cgaagtgctt ttgacaaaat cgtgatttct gaagctttga tagaaacttc gcctgtgtct
     1141 gttttcatga tttgtccagt tacaccaaag aaatctccaa ggtctgcatg tttgaatact
     1201 tcataggcct catcacctac ttggtctttt cttacataaa tttgaatttg gccttcacga
     1261 tcttgtagat gcgcaaatcc agttttccct tttcctcggc gagtcatcat acgaccagct
     1321 acactagcgg tcaatcccat ttctgctagt tcttcttttg ttcttggatc aaatagttca
     1381 tgcaattctt ctgagttgtg cgtgcgttca aatcgttttc caaatggatc gattccatct
     1441 tcacgcaatt gttccatttt ttgacgacgt acaagcattt gatcatttaa atcttctact
     1501 tgatgttgtt gttcctgtgt cacaaaagtt cctccattct catgttaatc tctttcctat
     1561 aatgccaatg aaacacaaaa aaagcaagcc gaacttggaa tttcggcttg aaaattcacg
     1621 aaaaacttag aaatcacaac gtttgtttca atagtacttt ttccgctttt tctacaaatt
     1681 gatccagcag gtcattgatt tcttgctgtt tttctgtctg attgattgct actttcactt
     1741 tcgctgctcg aggaatccct tttaaataat aagccgcatg tgttctgaat tctctacaag
     1801 ctactttttc tcctttgaga tctgctaagc gttgtaaatg aagtttagct gtagcaattt
     1861 tttcggctgg tgttggttca ggaatcagtt ctcctgtttc gagataatgc tgtgtgcggt
     1921 gaatcatcca tggattgccc agcgctgcac gaccaatcat cacaccgtct gcaccgacat
     1981 agtcaagcat ccattttgca tcttctggcg ttttgacatc cccgttcccc ataaatggga
     2041 tcgtcaaatg ttttttcact tctttcaata cttcccagtc cgcttttcct tcatacatct
     2101 gaacccgtgt acgtccatgc atagcgatgg cacttgctcc agctgattgt gctgctaatg
     2161 cattttctac agcaaagacg tgctcttcat cccaaccaat acgcatcttg attgttactg
     2221 ggatatttac tgctgaagaa acagcttgga ccatttcgta tactttctca ggatctaaaa
     2281 gccattttgc tcctgcttct gctttgatta ctttatttac tggacagccc atattgatat
     2341 ctattatatc ggcagctgtg ttttcttcga caaattgagc agcttcaact agactatctt
     2401 tatttccgcc aaatatctgt aaacttaatg gatgttctgt ttcttcaatg tggagcatct
     2461 ctaaagtttt tttgtttctg aaatgaatac cttggtcact gatcatttca catacgacta
     2521 agcccgcacc aaattctttg acagtgactc gaaatgctga atttgttatt ccagccatcg
     2581 gtgcaacaac gacacgatta gggatttcca cattgccaat tttccatgtg tgttccactt
     2641 aaatccctcc taaggcttct ttcttcaatc catctaattc ttcttcgcta tacttgtatt
     2701 tgttattaca gaaagaacaa accgcttctg caccgtgatc ttcgtctatc attgcttgga
     2761 tttcttctgc acctaacgcg ataattgctt ctgcaaattt ttcttttgaa cagtcacatt
     2821 tgaattgaac aggcattttt tccaaaatgt tcaattcttc tgtaccaagc aatcctttca
     2881 atatttcttc tggtgtctgt ccttcatcta agagggtaga gattcttggt gtcgttttta
     2941 attgttcttc gatcttgctg attgtttctt catcggcacc tggcatgatt tggatcatga
     3001 atcctcccgc cgtcttgatg ctgtcgtcag tattgaccaa tacactcagt ccaacagctg
     3061 aagggatttg ttctgagata gcaagatagt aagtaaaatc ttcagcgatt tctccagaaa
     3121 caatcggcgt ttgcccagaa aaaggttctt ttaatcctaa gtcttttgtg acagtaaaca
     3181 ttccttgtgt tccaactgct ccccgcacat cgattttacc ttcactattt agaggaagac
     3241 tgagatgcgg atttttgatg taacctttca catctccctt tccatttgca tctactacaa
     3301 ttcctgttgc tggaccatcg ccttggattc gaacagttag tttgtcttct cctttgagcg
     3361 tcgcaccaag tagcagacta ccgaccaatg tccgtcccaa agcagctgag gaggtgtgcc
     3421 aagtatcatg acgtctttgt gcctccgcta cggtatttgt tgcttgaacg gcgtatgctc
     3481 ggacgaatcc gtcgtgagca agtgccttaa ccaaataatc tttcatttgt tatgtactcc
     3541 tttttccgtt tttaaactca tagtgaatca tactagcaac tactgctttt ttcaactttg
     3601 gaatgcttct tttaaaaata attcagaatc atctagaaaa caggacatca ccctatttac
     3661 aacttaaata aagaaatctc ccgaaaaccg gtttttgttc cggttttcgg gagtcatatc
     3721 agatcaattg acctatattt ttttatttgt attgatcgtc tgaatgatta tcttctgatt
     3781 gttgagaatc tgaatcatgt tctgctttat tttcctcatt cagttcatgt tttgcttctt
     3841 caaaatcacg tttttcttcc gcttgttttt cagcgtcttt ttcttccaaa gcacgttttg
     3901 cttcttcaaa agttttcgct tctttttcgc ttggataatc atttgactca gtaccttcag
     3961 gcatcacacc gtgttcaaac aatgacttga ttgctttggc atccaatgtt tcgaattcca
     4021 aaagtttttc agcaatcagt ttatgctgtg cacgatgagc ttcgatgatt tctcgagctt
     4081 tatcatgagc ttccatcaag atttttcgta cttcttgatc aatctcgaaa gcgacttgtt
     4141 ccgagtaagc ttttgtttga ccatagtcgc ggccaacaaa cacttgatgg tttccttcat
     4201 attgaacagg acctaaacga tcactcattc catattctgt taccatgctt cgtgctagag
     4261 ctgtcgcttg ctcaaagtca ttggaagcac cagttgattg gacattgaag atgatttctt
     4321 cagctgtacg tccaccaagt aagcctacga tttgttcaaa catatcttct ttcgtcatca
     4381 agaactgatc ttcttttggt aaagcgatca tatatccgcc ggcgcgtccg cgagggatga
     4441 tcgttacttt gtggacgacc cgagcgcgac tcaatactaa tccgacaatc gtatgaccag
     4501 cttcgtgata agcaaccatt tcgcgttctt ttttgctgat tacgcgatct tttttagctg
     4561 gacctgcaat cacgcgatct tccgcttcat cgatatcaga agcatcgatt ttctttttat
     4621 ttcttctagc agctactaac gctgcttcat tcaagacatt ttctaaatct gctccggcaa
     4681 aacctggtgt ctgttgagcc acaactttca agtcaacgtc atctgccatc ggtttattcc
     4741 gtgcatggac tcgcaagatt gcttcacgac cttttacatc tggacgtcca actaaaatct
     4801 ggcggtcaaa acggcctgga cgtagtaacg ctgggtctag tacatcagaa cggttggttg
     4861 cagcaatgac gattactcct tcatttccgt cgaagccatc catttctaca agcaattggt
     4921 tcaatgtttg ttcgcgttca tcatgtccgc cgcccatacc ggcaccacgt tgacgaccta
     4981 ccgcatcaat ttcatcaatg aaaatgatag ctggtgcgtt tttcttcgct gtttcaaaca
     5041 aatcacgaac acgacttgct ccaacaccga caaacatttc tacgaaatca gaacccgaga
     5101 tcgagtaaaa cggtactcca gcttctccgg caacagcttt tgctaataag gttttacctg
     5161 ttcctggagg tccttctaaa agtacacctg ccgggatacg ggcacctaat tcaacaaatc
     5221 ggcgaggatc tttcagaaat tcaacaactt caactagttc ttgtttttct tcttccgcac
     5281 ccgctacatc agaaaaacgt acgcgattag cttttttgtc tgcttctttg gcttttgatt
     5341 taccaaagtt catgacacgg ccgccaccgc caccgccgcc gccttgttgt cccatcatca
     5401 tgtaaaagaa aaagatgatg attacgattg gcaagaagct aagcaatagg gaaacccata
     5461 taccgctgct tgattcttct tttactgttg tagcaacttt gttttctttt gccagttctg
     5521 ttacttggct caatgttgta tcatttggca aaatgattgt tgtgaaatgg gtagtagtcg
     5581 tatctgttga acctaagata gctaacccac cgctgttagc gacggtctgc tgttctttgt
     5641 attcaccagt gatcttatat acaccatttg caggctgcac ttcaaattct ttgatcttgc
     5701 ctgcttgtag ttgttctgtg aattttgagt actcgatatc tgacgatcgc tggccgttat
     5761 taccaaaaat gaaataaacg accattacca tcgctaagat tacgaggaca taatacaagg
     5821 catttttcat catgccgttg ttctttttgt tcatagttgc cctccctaag cgtcaattta
     5881 atttaacagt tttttatcaa tcctgaccat tctattgtac cataatttaa ttgagataca
     5941 gcattaattt gattgataaa cactaggttt caatacaccg atgtacggaa ggttacgata
     6001 tgcttctgca taatccaagc cataaccgac cacaaattca tttggtacgt tgaagccaac
     6061 ataatctgct tcgatattta ctactcttcc ttcaggtttg tcaagtagtg taacaatttt
     6121 gactgatttt gctttgcggt atttgaaaag atccactaaa taagcaagtg ttcttccgct
     6181 gtcaatgata tcttcaacga tcaacagatc gcgaccttct acattcgtat ctaagtcttt
     6241 gacgattttt acttcccctg acgagactgt tgcattcccg tagcttgaaa catccatgaa
     6301 atccaattcc aaatacgtat cgatagaacg aacgatatct gccatgaatg gtaccgctcc
     6361 ttttaagaca cctactacta ggggattggt gttttggtaa atttctgtta actctttgcc
     6421 tagttctttg caacgaactt gtatctcttc ttgtgagatc aaaattctct caatatcctt
     6481 ttccaacatc aggttctcct tccaattgct tctttttgat atttgtaaag aagtatgtag
     6541 tgtattttat cagtttcttc acgaatactc aaatatgaaa aggcaaaagg tatcaagcta
     6601 tatatatttc ttttggcatc tgtaatgatc caagcatgct gcctttgtga attaggtact
     6661 ttctgatcga taaaatatct agatactttt tttgtgagat tctctgttaa tttaatgcgg
     6721 tctcctgctt ttcttttttc gacatacagt tgcacatcag caggaatcca aatgtcatga
     6781 gaaaatagag accatgaatc tgttgctttg atgtcccctg cattttcagg tttgaccaga
     6841 ccaatccatt ctgtttcatt caaatatatt ccttgttctg gaatcagcaa caagcgctgg
     6901 tcttctgtct ggctagttgc ttgtttttct tttttagaga gtacaacaaa atgatactct
     6961 cttttcagta tccaattatt gccaatactg agttgccatt ggcttttttt ctgttgccac
     7021 tggtctaaaa tcgactccat ttgttgttct ttcaaaataa ttcctgtttt tgagaatgct
     7081 ttgtcgaaaa aggagtaaaa aaaatagtat cgttcagcat ctgtcatttc ttcaatcatt
     7141 tctgcagaaa atcgaaattg ttcctcccac tgttcgactt ccttttcgat caactgtccc
     7201 atatattttt gaatgacttg gtctgcccat tggatctgtt gcgaaaattg ctggaaatgt
     7261 ctcaacgcct gtgtattttc ttgtttcaat tgaggaacta ccaaatgacg aagccgattt
     7321 ctctggatat ctaacaattg gtttgtctga tcctcaaagt aaacaagctg cgattccgct
     7381 gcataagtat ataggtcttc tttgctaaaa ctcagcaacg gacgaatcag tctgcccgta
     7441 gcaaatggtt gtacttcttt gattccagaa aaagtattta actgtccccc acgaacgatt
     7501 ttcattaaca ccgtctcgat ttgatcatct ccgtgatgag cagtcatcaa cgtgtcatat
     7561 tgtccttctt ccatcacgcg agtgaattct tgataacgaa agttacgagc ttctgtttct
     7621 atggcggatt tggctggatc ttcccaaata cttacatata aagggagatt cttctcttca
     7681 caatatgagc gaagataggc agcttcttct gctgattctt ctcttaattg gtgattgatg
     7741 tggacgatac ctaaagaaaa accgatttgg cgttgcactt gtaaaagtaa atggagcaaa
     7801 accatcgaat caacaccacc tgataccgct gctaaaattc tcgatccttg ttgccagaat
     7861 ccttgttcat tcccgtgccg aataaatgac tgctgtaata gcggtgcttt tttcccgcct
     7921 ttttcaacgt tcatcctttc cacacccttt cttcctaaaa aaaagagagc gggaaaacgt
     7981 ctaaaacgac aattaacctg ccctctgttg atactatgac ccaaatagtc gcactatcat
     8041 tatttgcggc tttttagctg cgacgtccgc cacggccacc gcgctttcct tctgtgtttc
     8101 gcttcaatga agataagcgg tcatcgctat ctttcaaaaa tgaactcatc aatgaatcga
     8161 agtcttcttt ttgagctgaa ggttgactgc gagaaaaaga ttttttctgt gctggacgat
     8221 ttcggttgtc acggggatct ttttgttgat attttttagg acggctttct tctccagatt
     8281 cagcaggttg ttcctgcgct ttccggatag atagtccgat ttttccatca tcatttactg
     8341 aaataacttt aactgttact tcatctccta cacttaaaac atcatggatg tcttttacat
     8401 agccatttga cacttcgcta atatgaacta gacccgtttt tccagcacct agatcaataa
     8461 acgcaccaaa atttgtgatt cctgatactt ttccttgcag ttttgctcct acctcgattg
     8521 acataaaaaa aatgttcctc ctaattattt ccctttttta tattcctgct tattttgcag
     8581 ttgtagaact agttgattga tctgtttcat ttgtcgagct gtctgtattt gattctaata
     8641 taggataaac tttttcaccg tcttttgagt aaaagaattt gcttctggca agtttagcta
     8701 catactcatc atctttcagc aatgaaacat cttggtgcag ctgatcaacg tctgcgttga
     8761 cttctttcat ttcagtattt gcttcgactc tcaattcgtt caattgatgc atcctttgca
     8821 tgtcatggaa aatctgcaca cctattatcg cgaaaatgac caaagcacca acaaaaattg
     8881 cagccagtct gcgtcttcga aaaatcagtt gacgttgttg ttgctgaaat tcagcatact
     8941 ttttctttgc atattccgta tctagcgcag cgattttatt gctttttttc gtcacttgga
     9001 ttcgctccaa tctttttgat tgtctttttc attataccaa tatcgtgaaa gcttgtctaa
     9061 tctatttctc tagtgtttct gtgcgatttt cagaaataat ttcgtacatt ttttgtgcat
     9121 cttctttttt ggttgattca tgtaattccg taattttcac ttccaacgtt ttgttgccaa
     9181 actggacttt caaaatatcg cctactttga catcactgga agatttcgcc aatttaccgt
     9241 tgatttgaat tcttccttta tcagctactt cttttgctac tgatcgtctt ttgatgattc
     9301 gggaaatttt taaaaattta tctaatcgca tatcttattc ctcacttttt aattttttta
     9361 tttaatcgca ggattttctt accaaaaggc aacattagcc attctcgtac tgtaaacaga
     9421 cgaagacgaa tcactgcata aatgaacaca gaagccccga tcccaactcc tatcacgctg
     9481 acaaaaaaag cagtggaacg atgagttaca ggaccaaata ctatattcaa tacaccataa
     9541 tacagcagca gtactactgt catacaaaga aggctgaaca tcaacttccg tccaaaatgc
     9601 cgttctttcc aaaaagcatt tagctctttt gtttcaattc gaacaaaata ccaaagcgtc
     9661 acacctaatc ctaaaagggt agacaggctt gcacctgctg ttcctaataa accggtcaga
     9721 aaagaagtag tcaatccttt taccaatagt ccccatcctg cgccttttaa agaagggcga
     9781 aacgagttct tgctttgagc aatactttga taagcttgga tgactgcagt gaaagcaatg
     9841 gaaaaaacaa ataatacaag cgctgcattt cccgcataat ctttaaacaa cgcataattg
     9901 atataaggga gcagcaaggc taatcccaaa gacgcagcta atgctaaagc ggtcgtcaat
     9961 ctaagataga ttttggctgt ctgtaaaaac tgacgataga tccggttggt caaatgacga
    10021 gtcaacgcag gcaggaatgt cgcacttaat gcagtggcaa tcactaaacc taattgtact
    10081 aaaggctgac ctcgatcata gacccctttt gctatcttcg caccgtattc tgacagtccg
    10141 taaacttcca aagcattttt tacaaagaaa gagtcgatca gctggaaaaa gataagcaat
    10201 ccactataaa tcgaaaccag tccgccttct accagaaaac gccggatcaa gggctttgac
    10261 ggctgtttga acaatggaaa ccgtcggaag cttaacgctc ctccatgaat cttttgttca
    10321 tagtagtgaa ggatcccata tgcgcataaa ccgccaaaaa cagctccact cattgctgcc
    10381 gtccctgttt ggtacaccgt ccatccaagc tttctaaaag caaacgcact acagacaatc
    10441 acaaaaacac gaacgaactg ctcaactact tgagaaacag cagttggttc catcaagaat
    10501 cttccttgga aattccctcg ataaaaagat agaccaggca tcagcaaaaa ggtaaaggaa
    10561 actacttgga tcagcggttt caattgtatg tctcccatca tccaagcaat ccagctgcta
    10621 aatgcaaaga caaacaacca caaaaagaca cctgtccaaa atactaaggg ataaagattt
    10681 tgcaaagctt gctttcgctc gaaaggattc tttctttctg caacgtactt tgatatgaac
    10741 tgcggaagac cggataaagc taaagtcatt gctatcccat aaattggata aacttgctga
    10801 taaacataaa aaccttcgtc gccgactaag ttttggaggg gaattcggta aagggcactt
    10861 aatacttttg ctatgaaaga agcaatcgtc aatacaaatg ctccttgcat gacgcgccgc
    10921 atttctttat gatccaaatc attgctcctt cctacgctga cagatatttt tgctcacgca
    10981 acgctttgac aaacttttgg atttcctgaa gccatactgc ttcttccatt tggttgggga
    11041 tcgtcaagcg tatcaccatc tgttcttttt caacgcctag actggctttt agttttgtcg
    11101 ctgtcagtgc ttcgaataac tgttccacac tatagcgttt tgttccaact ttacttagag
    11161 taaagactat atgcgcctcc tgtttcctta tcgtttctag gagcgctcga tcgccatcca
    11221 ttttgatctg cccaattgtc aagagatgtg ctacttcttc tggatattct ccaaaacggt
    11281 cgagcagatc atcttctagt tcatctagca tttcttgcga atcaagttca cgaattcgtt
    11341 tatagatctc gattttctgc cgttgatctg ccacgtatgt ttcaggtaga tacgcatcga
    11401 caccgaggtc gatctcaaca gtggtcttct ccacttgact attctttcct tgtttacgag
    11461 aaacagcttc gcttagcatt tgagaataca tatcgaaacc gactgcatca ataaatccat
    11521 gctgctgagc acctaagaga ttacctgcac cgcgaatgga aagatcccgc atcgcaatct
    11581 tgaatccaga tcccagttct gtaaaatctt tgattgcctg aagacgtttt tcactgactt
    11641 cgttaaggat cttctgctgc tcatacataa agtaagcata tgcgacacgg ctgcttcttc
    11701 ctacccgccc tcgcaattgg tataacgtgg ataatcccat ataatcagcg ttctcaacga
    11761 acaacgtatt tgcgttcggg atatctactc ctgtttcaat gatggttgta gtgacaagaa
    11821 catcatattg accttcaata aattcaaaaa gcgtattttc taattgcgcc tctgtcattt
    11881 gcccatgagc ataaccgatc cgtgcttccg gaacgagttc ttggatttct tctacttttt
    11941 gttcgatcgt ttctacccga ttatataaat agaatacttg tccacctcgt cccatttctc
    12001 gatggattgc ttcgcgaata gccccaggat ttttttccat aacatacgtt tggatcggat
    12061 aacgattcgc aggcggtgtt tcgattactg aaagatcccg cacgcctaac atcgacatat
    12121 gcaaagttct cggaatcggt gttgcagtca gagtcaaaac atccacttgt gcacgtaact
    12181 gtttcaatcg ttccttatgt tttacaccaa aacgttgttc ttcgtcaatg atcagtagac
    12241 ccaaatcgct aaattcaata tcttttgaca aaattcggtg ggttcctaca acaatatcaa
    12301 cttgacctgt acgcagttgt tcgatggttt ctttttgttg ctttcttgtt ctgaaacgac
    12361 ttaatagccc gatatttacg gggaaacctt caaatctttc cagcatcgtt tcatagtgct
    12421 gctgtgccaa gatcgttgtt ggaactaaaa aggctacttg tttgctttcc tttatcgctt
    12481 taaaggccgc tcgaagcgct acttctgttt tcccataacc aacatcacct acgagtagtc
    12541 gatccattgg tttttctttt tccatatccc gtttgatctc cgctgcactt ctcaactggt
    12601 catcagtttc agagtagggg aaggcatttt cgaattcttt ttggtaggcg tcatcaggtc
    12661 caaaagcata tcctttttct gactctcttg cggcatataa tttgattaga tcgtcagcga
    12721 tatcctcgat ttttgaagag acttttcgct tggttttcgt ccattcactt ccgcctagct
    12781 tattgatgcg aggcgctttg gcttcagagg cgacatattt ttggatcaga ttcagttgag
    12841 taacaggaat aaacagtttg tcgtcgtttt gatacaaaat agtcatatag tcttggtgaa
    12901 cgccatctac ttctagcgtt tccatcccaa tgtatttccc gataccatga ttggcatgga
    12961 caacgtaatc tcctgctttt aactcattat agcttttcaa tcgctctgcg tttgttactg
    13021 tttgacgtct cgtttgtttt ttcgtcgttt tttggaaaat ttctttttct gtgatagcta
    13081 ctattttttc ttgaggaagt tcaaaaccag cctgcaaagc tccttcaact aattgtacct
    13141 gtcctctgat caatgcattt ttttctgttt ctactactaa tatgtcttca tctcgaagca
    13201 tttgttctgc tttgcgtatt ctgtcctttg tcggaataaa gatcacaaca gtctgttttt
    13261 gcttgcgcca acggtccatt tctgttttca gcaatggcat ttgtccgaag aactgttgca
    13321 tcgtccgata ttgaaaatta tggattgctt ggaaacgaag atttcccatc cctttttgga
    13381 agagtgagaa aaaggttgtg gtgaactttt ccttctgcac gattttttgt atatctgctg
    13441 aaaatgtctg ctcagaaaac acccgcaatt cgctgatttt ttgagtctgc cattctgccg
    13501 cttccctttc gatttcccga tttgtttcca tgattcgctg ataatcatca acgactagta
    13561 aactatttga agggaaataa tccaagatcg tcacagattc ttggtaaaga agatctgagt
    13621 agaattttgc ttcttctgtc gggacacctg cttgccagct tgaagtcagc tgaccgaagt
    13681 aatcttgcaa aaactcgcgg tctgcttctt ctgtggtcgc agcaagccgt ttttctagca
    13741 ttttttgcat ttgctctatt ccatgttcta gatcctctgg tccgtagact tgttcactcg
    13801 ttggggaaac ccagatttct tccgtttttt cgattgagcg ttgtgtttct gcttcaaaat
    13861 accgcataga atctatctct acatcaaata gttctacacg aacaggatag gttgtcgtta
    13921 acgggtaaat atcgataatg cttcctcgaa tactgaattc tcccggtttt gcgaccatct
    13981 cttctcgtac atagcccatc aaagtcaatt ggcgaggaag attttccacg tctatctcat
    14041 caccaatatg ccaattcaat tgcgcctgta gccaagtttt cttattcggc agtcgtttgt
    14101 gcaaagatgc gactggcaac aaatagatac ctgctttttt ttcttgaagt gcgttaagcg
    14161 ttgcaactct ctcgcctcgc gcttctggag aagaaaaggc catttcagca gaaagcactt
    14221 catccactgg aaataaatgg atctgatctt gcggaatcac atgttgcaga tcttctgcta
    14281 actgattcgc ataatacaaa tttggcatca gaataatcat gtttttattt gttttcttaa
    14341 acgctcgagc aaatagaagt gttttcgctg agcccgctaa tcctgtgatt agttgtctag
    14401 gcattttttc ttctaaattg ctgacccatt cttttacttg cggatctttt ccgactaatt
    14461 gaataatatc cattcctttt cctccttcct caattgaatc gattcatcgc atccacgaac
    14521 gaatgccctt ctatgagata ttcgactgct ttaacagatt gacacatgct ttcttctatc
    14581 aaaggctgat tttctttgct gaaaggacta agcacatgct ggactactgt ttttttccct
    14641 tctggcctgc cgattcccac cttgattcga tcaaagacat tcgtattcaa atgggaaata
    14701 atacttttga taccgttatg cccgccggca cttccctttt gacggagccg gatttttccc
    14761 actgccagat ccaaatcatc gtagattacg actaattcct ctggataaat cccaaaataa
    14821 gtcattagtg ggccgactgc acgaccagat tcattcatga atgtttgtgg tttgactaaa
    14881 aggatctttt ctccattatg aaaaaattcg gcaacttctg cttcgaacgg attttttttg
    14941 aaagtagctt gatgctcgcg tgcaatttta tccacgacca taaagccgat attgtgtttt
    15001 gtatattggt atttggtacc tgggtttcct aacccgacga tcattttcat ttttgttttc
    15061 cacctaattc taaaaagcag cacaaaaggc tggactgcta attagcggtc cagtccatcg
    15121 agcttttttc ttttttatac ttgtcaatgc atagtataac acaaaacaag gcgtttcggc
    15181 taaaagagaa tcgttaataa atgataaaga aattaaaaat ttcttcaaag tttaatgaaa
    15241 taattgatat ataaaaatag tgaaataacg cacatgtaag cttttacata taatacaacc
    15301 ttgttatctg ttatatttta tagccaaata gcgtgacatg gggcttgaga aatgttagca
    15361 taagcatggg aaaaaacaaa tcaatggaag gattgatact aaatgactgc agctgcagaa
    15421 aaaaaagatc atcaaaaagt tattcttgtt ggagacggcg ccgtaggttc tagctatgcc
    15481 tttgctctag ttactcaaaa tatcgctcaa gaagtaggta ttgtggatat caatactgct
    15541 aaaactgaag gagacgcaat tgacttgtct cacgctttgg catttacttc acctaaaaaa
    15601 atctattctg ctacttacgc agacgcacat gatgctgact tagttgttat tacagctggc
    15661 gcaccacaaa aaccaggtga aactcgttta gacttagtaa acaaaaacct aaaaatcaac
    15721 cgcgatgttg tcacacaaat cgttgaatca ggatttaacg gtatcttttt agttgccgct
    15781 aacccagtag atattttgac ttactcaact tggaaattct caggtttccc taaagaacgc
    15841 gtaatcggtt caggtacctc tcttgactca gctcgtttcc gtcaagcact tgcggaatta
    15901 gttgacgttg atgcacgtaa cgtccatgct tacatcttag gtgaacacgg agattcagaa
    15961 ttcccagttt ggtcacatgc aaatgttgcc ggcttgcaaa tctatgaatg ggtaaaaaac
    16021 aacccagaaa tcgatgaaga agctatggtt aacctattct ttggcgtacg tgacgctgct
    16081 tacacaatta tcgagaaaaa aggcgctact ttctatggta tcgcagtagc attagcacgt
    16141 atcactcgtg ccatcttaga tgatgaaaat gctgttcttc ctttatcagt attcatgaat
    16201 ggcgaatatg gattgaacga catctacatt ggtgcacctg ctgttatcaa ccgtcaaggt
    16261 atccaaaaag taatcgaaat tcctttaagt gacagcgagc aagaccgtat ggctgcttct
    16321 gctaagcaat taaaagaaat tctagatgaa gcttttgcta aactagacgc tgaataatct
    16381 ttgaacatga cggaaaacat ctattcggtg ttttccgttt ttttatttta tcacccttat
    16441 ggtttctaaa ttttttcata aagtagagat aaccttcgtc ttttcctcta aattatgcta
    16501 tcatgagacc gttccaccct aaacttttac ttaaggagtt gtcgatatga caagatcttc
    16561 gcatcgaaaa aaatcgaaac atgttgcttt gctttcagtt cttggtgttc tcttagtgct
    16621 gatcagcttt tatacctatc gcagtacata ctacacaaag cattttttgc cgaatacaga
    16681 aataaatgga ataaatgtca gcaatctaac agtcgaaaaa gcaaatgaaa aattaaaaga
    16741 agcttattct gataaaaaac tggctattaa agaagatgga aaagtctggc aggaagtacc
    16801 aaaatccgag ctcggctaca aagatgattt cacttcggag ctttctccga tcctaaatga
    16861 acaaaacgga tggacttggg gaatgactta tgtctcagcg gcagaaaaac aagaaataga
    16921 tcctgtcagt caaaaccacc aaaaactaga cactaccgtt cagacattga caactaagct
    16981 gactgatttg aacaaagacc ggacacctac tactgatgca acgatcgaaa aagcaggtga
    17041 ctctttcgtt atcaagcctg aagtcaatgg aaatacgatc gacgtcgatg ctgcggtcaa
    17101 acaattaaaa agtgcggtaa actctggtaa agatacaatt gaattgactg aattcaaaga
    17161 aaaaccaaaa gtaacatctg aagacagctc actgaaagaa caactcgcat ccatgaatgc
    17221 tatcgcaaat gtaaaagcta cttacagtat aaacggtgag actttccaga tcccatcaag
    17281 tgatattatg agttggctga cctacaatga tggaaaagtc gatttagata cagaacaagt
    17341 acgtcaatat gtaacagatt taggcacaaa gtataacaca agtacgaatg acacaaaatt
    17401 taaaagtaca aaaagaggag aagttacggt cccagttggc acttacagtt ggacgatcca
    17461 aacagacagt gagacagaag cattgaaaaa agcgattctg gcaggccaag attttactcg
    17521 ttcacccatt gtgcaaggtg gcacaaccgc tgatcatcca ttgatcgaag atacgtatat
    17581 cgaagttgat ctggagaacc agcatatgtg gtattacaaa gacggaaaag tggcccttga
    17641 aacagatatc gtttccggaa agccaactac acctacacct gctggagtct tctatgtttg
    17701 gaataaagaa gaagatgcaa ctttaaaagg aacaaatgac gatggaacac cttacgagag
    17761 tccggtaaac tactggatgc cgattgactg gacaggtgtt ggtatccacg attcagactg
    17821 gcaacctgaa tacggtggag acttatggaa aacccgcggt tcacacggct gcatcaacac
    17881 cccaccaagt gtaatgaagg aattattcgg gatggtcgaa aaaggaacgc ctgtattggt
    17941 cttctaacaa aaataatata taaaaagaaa gcacccgtac gattggtaca ggtgctttct
    18001 ttttattatc taacaatgtg ctacttacat ttcagcagca gtaaagactt ctgaaacatc
    18061 atcatcgtct tctagcttat ctactagacg ttccaattgc tctttttgtt cctcatttaa
    18121 atcaatcgtc gtttgtggaa tcatcgtcaa ttctgcttgc gccaaagtga actctttttc
    18181 caactcatct cgtacagcag caaaatcttc tggtgcagta taaatctcga atacttcagg
    18241 agaagtttgc agatcttctg cccccgcctc taatacatcc tcaaacatcg tctcttcatc
    18301 aacagtcaac ccatcgcgtt caatagcgat atatcctttg cggtcaaaca agtaactgac
    18361 tgaacccgtt tctcccagtg atccaccgtt tcgagtaaaa gctactcgga catttgtcgc
    18421 tgtccgatta cggttatctg tcaatgcatg gactaagatt cctactccac ccggaccata
    18481 tccttcatac gtgatttcat cgtaattcgt ttcatctacg gcactcgtag cctttttaat
    18541 cgcccgttcc acattgtcgt taggcatatt tgcagctttt gctttgtcca caacaagccg
    18601 caaagctgga ttcatagatg gatcaggacc gcctgcttta gctgccatat aaatttcacg
    18661 agaaagtttc tgaaagactt tccctcgttt tgcatcttgg gcattttttc ttccttggat
    18721 attatgccat ttactatgtc ctgacatgat ttttcctccc ttgttctttt gagaatacac
    18781 ttctattcta gtactatttc acaaaaaaac aaagccacag ccaatctata aacgcttctt
    18841 gaatgccgca aaaagcacac ataatatcac agcagtcaga gcaccgatcg aatccaacat
    18901 gacatcttgg aacataggtg ttcgtccacc ggtcatcatc tgatggaatt cgtccaaacc
    18961 tgcgtaccct gttgctgaaa gccaagcaag gatcgctgtc agccaccaaa tttttagctt
    19021 ggggaaaaca cctaaaaaca agctgcctcc caaaataaaa taggtaccga aatgtgcgcc
    19081 cttccttaaa aagaactcta aaaatttggc gtagcccaga tggtcgatac tcaccatact
    19141 tccaccataa ttaaaacgta tacccgctaa tgtgtcttta aatggctgat taggcagcca
    19201 gctttctaat cgcgaaactt gtgattgctg tccgtaagtt tgagaagaac tgtaaaataa
    19261 gattataacg actatcagtg cgattactaa ataaatattt ccgtttttca tttgtttttt
    19321 tgaagaattc ataagtcacc tctgtatttt atcttagact ttttttgaag aaacaacaat
    19381 cattttcata gagacttctt ccataaaaaa gtgctatact taattttgta ttacaatatg
    19441 aagggagttt tttcatgagt tattcattaa attggaatct agattctatt ttttccggag
    19501 gcagtcattc tgatgctttg aaccaacgga tgaagcaatt ggaagaccaa atgaacgagt
    19561 attatcaccg cgtaacaaaa tggtcaccta gttctgacaa tgcagagcag ttaaacgcta
    19621 ttcttcaatt gcaagaaacg attacgaatg gttttaccca atgcagcagc tatattactg
    19681 cattgctttc tgccaatgtc aatgattcag atgcaaaggt cttatcaggc aagctttacg
    19741 caatgctgcc tcgtctgcaa tcagccgaaa ctgtattgtc aaagaaattt gcagaaatat
    19801 ctgacaatga ttggaatcag atgctttctc aatcttcgtt tgaaacaatt gcttttcgtt
    19861 taaacgagat tcgtcgtgat ggctcacaat tattatcaga agctgaagaa aacattataa
    19921 atacgctttc tttagatggt ctaaatgctt ggagcagtca ttatgatacg attgtagcca
    19981 gcatttcgat tcccttcgaa caagatggac aagtagtcga attatctgct ggacaagcct
    20041 tcaataagat gatgggcgat ccagatccaa aagtcagaga aacacttttc gcagcatggg
    20101 aagaagcctg gaaagaaaag gcacccctct tcacggatac tttgaatcat ttagatggtt
    20161 ttagactttc tgactatgaa cttcatggtg tcacagattt tctacaaaaa ccattagagt
    20221 acaatcggtt gaagaaagaa acattgtctg tcatgtggga tacaatccag aaaaacaaac
    20281 aaccccttgt taattacttg actagaaaag ctaatctttt cggtaaagaa aaaatggaat
    20341 ggcaagatca agatgcacct attattttag gcgatttgaa agaaaaaacc tttagttttg
    20401 acgaagcagc agcatttatt atcgaaaact ttaacaaatt cagtccaaaa atggctaaat
    20461 ttgcccaatc tgcttttgaa aagagctgga tcgaagcaga agatcgtcct gggaaacgtc
    20521 ctggtggtta ttgtactgag ttaccagaaa caaaagaatc aaggatcttt atgacctaca
    20581 gtaattctgt taatgaggtt gcgacacttg ctcatgaact aggacatgct ttccatagca
    20641 gcacgatgtg ggatttacct tctttaaatc gcgaatatgc catgaatgtc gcagaaacag
    20701 caagtacatt cgcagaactg atcgtagccg atgctacatt aaaagtcgca aaaacaaaag
    20761 aagaaaaaat caatttgcta gatacaaaac tgcaaaatgc tttagctatg ttcatgaata
    20821 tccattcccg attcattttt gaaaatcgat tctatgaagc acggcaagaa ggattagtct
    20881 cagaagataa gatcacagag atgatggttg aggcgcaaaa agaaggctat caaggagctt
    20941 tagctactta ccatccatat ttctgggctg caaaattgca tttctttatt gatgacgtac
    21001 cgttttataa cttcccatac acatttggct acctctttag tttgggcatc tatgcttacg
    21061 ccaaccaaaa gggagcaaat tttgaagaag aatatatcca tttactacgc gatactgcct
    21121 caatgacgac ggaagatctt gcgaagaaac atttgaatgt cgatcttacc caaccagatt
    21181 tttggcaagc aggtatcgac atggtgactg aagatatcca aacgttcatg gaattgacag
    21241 aagaatttat ctgatttcta ttttatcaca ataaaaaaag agttagtcca gaatgggcta
    21301 actctttttg attaatcatt cttacatgct gacagcaaag tcaggggcaa accattgaac
    21361 tgaaccaact tttacgcttt cgcctggttg aggagcatgg atatattgtc ctccgcctaa
    21421 agcaatcgct acgtggtaag ttccgccttg tgacccccag aataacaagt ctccagcttt
    21481 tgcttgagaa actgaaattt ttgttccagc tgattcttga ggaactgtcc aaccaccgat
    21541 gtcacgacca gttacttgca tgtaaacata gcgtgtgaac cctgagcagt caaagccact
    21601 tggatcttta ccgccccaaa catatggagt accaatatat ttgtaagctt ctgctacgat
    21661 tgctgcacca ttcacacttc cagaaggagc tggagcaggt gttacaggtt ttggttgttc
    21721 aggtgttggt gttggcgttg gagccggagt actacttcct gtgccgtttc ctgtatcaac
    21781 actttgatct gttgatggtg ttgtaggcgc aggtgttgtt gattcttctg ttgttgaaga
    21841 ttcaggcact gttgttgatt cttctgttgc tgaagattca ggcactgttg ttgattcttc
    21901 tgttgctgaa gattcaggcg ctgttgttga ttcttctgtt gctgaagatt caggcgctgt
    21961 tgattcttct gttgaagatt caggtgttgt tgattcttct gttgctgaag attgtgtagc
    22021 tgatgatgat tcttcagtca ttgaagattg tgtagctgat gattcttctg ttgctgagct
    22081 ttgtgcagta gatgaagctt gagtagcttg tgcttcagcc tctgcttgtt cgcgtgcttc
    22141 tttttcagct ttttcttgtg cagcttgttg acgagcttgt tctgctagac gttgttgttc
    22201 acgaatacga gcttgctctg cttcagcttc tgctttttga cggttcaagt cagctttctt
    22261 gtcttcagca gttgcttgtt cagcagctaa tgaagttttc agtacgttca aatcagcttg
    22321 tttagataaa agatcgcctt tttgagattc caaagcagct tggttttcag ctaattcttt
    22381 tagtttagca tcgttttcag cttttttgtc ttctacagct tttttatctt gtttttgttg
    22441 ttccatcaag tcgttgtttg cttttaccat cgttgtcatt gcttgaacgc gtccaatagc
    22501 atctgctaat gaatcagcat tcaatacagc atcgatgtag tttgagcttg tgttgcttac
    22561 ttgtgcttcg cgtgcttgtt tttggattgt atcttcacgt ttttcaatac gttcttgtaa
    22621 atctgcaatg tcttttacta attgtgcaga ttcttgatgc aatgtatctt gttttgctaa
    22681 taggtcttgt gcttgtgtgt tgatttcaga aacttggctt tctaaggcat cgatttgtga
    22741 ttgtgcatct gcttgttgat ttttcaagtc tgcaatcttt tgatcttgct gttgtatctg
    22801 agaatcaaaa tcgtccgccg cggcagcgat cggtgatgct acggcagtca aggtcattga
    22861 acataccatt actgctgata ttaaactctt tttcactctt cattcctccg actggcttaa
    22921 tttaataaat atctaaatat atttaagtct ttcttaacga tataaaaatt gtaccatagc
    22981 agtgtgtcaa cgatataata gttatgttac aaaaatgttt caaaacaact tttaaaaatg
    23041 acaaaactgt tcgtttcaga aaatgatcat gaaaaagccg cgttaagtct a
//
LOCUS       DAXWOO010000032        22755 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.032, whole genome shotgun sequence.
ACCESSION   DAXWOO010000032 DAXWOO010000000
VERSION     DAXWOO010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22755
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.032"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            109..3387
                     /locus_tag="ACGE70_000965"
     CDS             109..3387
                     /locus_tag="ACGE70_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFF8692616.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3928..5835)
                     /locus_tag="ACGE70_000966"
     CDS             complement(3928..5835)
                     /locus_tag="ACGE70_000966"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFF8692617.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5965..6840
                     /locus_tag="ACGE70_000967"
     CDS             5965..6840
                     /locus_tag="ACGE70_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFF8692618.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6920..7894
                     /locus_tag="ACGE70_000968"
     CDS             6920..7894
                     /locus_tag="ACGE70_000968"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFF8692619.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8074..9219
                     /locus_tag="ACGE70_000969"
     CDS             8074..9219
                     /locus_tag="ACGE70_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFF8692620.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9356..9700
                     /locus_tag="ACGE70_000970"
     CDS             9356..9700
                     /locus_tag="ACGE70_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFF8692621.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            9971..11257
                     /gene="efmA"
                     /locus_tag="ACGE70_000971"
     CDS             9971..11257
                     /gene="efmA"
                     /locus_tag="ACGE70_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFF8692622.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVSYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11395..11994)
                     /locus_tag="ACGE70_000972"
     CDS             complement(11395..11994)
                     /locus_tag="ACGE70_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFF8692623.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12398..13519
                     /gene="mnmA"
                     /locus_tag="ACGE70_000973"
     CDS             12398..13519
                     /gene="mnmA"
                     /locus_tag="ACGE70_000973"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFF8692624.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13765..15186
                     /gene="arcD"
                     /locus_tag="ACGE70_000974"
     CDS             13765..15186
                     /gene="arcD"
                     /locus_tag="ACGE70_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFF8692625.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15200..16318
                     /locus_tag="ACGE70_000975"
     CDS             15200..16318
                     /locus_tag="ACGE70_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFF8692626.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16378..17670)
                     /locus_tag="ACGE70_000976"
     CDS             complement(16378..17670)
                     /locus_tag="ACGE70_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFF8692627.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            17820..18380
                     /locus_tag="ACGE70_000977"
     CDS             17820..18380
                     /locus_tag="ACGE70_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFF8692628.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18509..18886
                     /locus_tag="ACGE70_000978"
     CDS             18509..18886
                     /locus_tag="ACGE70_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFF8692629.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18819..20891
                     /locus_tag="ACGE70_000979"
     CDS             18819..20891
                     /locus_tag="ACGE70_000979"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFF8692630.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(21102..22049)
                     /locus_tag="ACGE70_000980"
     CDS             complement(21102..22049)
                     /locus_tag="ACGE70_000980"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFF8692631.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            22295..>22755
                     /locus_tag="ACGE70_000981"
     CDS             22295..>22755
                     /locus_tag="ACGE70_000981"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFF8692632.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQ"
BASE COUNT         7369 a         4147 c         4822 g         6417 t
ORIGIN      
        1 tctttttata aaatacactt attataattt tgttttttat tgacagtaaa aataaattag
       61 gtaatacttt aagtaaacgc tttctaattt gttacgggag gaatacactt gttaaaaaaa
      121 cgtataaaat acagtgcgct aatgatgaat ctccttatgc tcagtacccc ggttttaatt
      181 tctgtcgttg aagttgctgc taacgaacaa cagtctgtga acgaagaaaa tgcacacgtg
      241 aacattgaag ggatgtcaac aatggaggaa ataccaagaa ctagtgaaga tatggatacg
      301 gaagaaggaa atgatcctat gatgaaagaa tcagaaaatg aaaagacaaa aaagcaatct
      361 gaagaagcaa atgaagagtg gaaaggggtt gttttcggag aaagcacaag tgctagtagg
      421 tatgccattg aaccattaga agacggcgtg cggctctctt ccacgaataa cggcggaaaa
      481 ttccagtcaa gcggatcaga tggaatgacc tattattatc aagcaatccc caaagacatc
      541 aactttagga tgcgggcgac gatcgaaata gaatcttgga cctatacgaa tgcccaagaa
      601 ggatttgctt taatggtaag agacgcagta ccaaaaaatc atttgtttgg gcaggcattt
      661 tattcaaatt catttgccat actaggcagt cggatcgaat atgtctggga ctcggataga
      721 caagaagtgg tcaacacgag cggcagcaaa tatacgatgc gacttggatt ggggacacgg
      781 gcaatcacag gaatatcatc tgaagatccg caagctgcgc cagctcctgg gagtgtctca
      841 gtggaaacaa cacctttaga gacttcagca gggttcttgg aaaaagaaac aggttcttat
      901 aatatattcg gaaacggtca tgggaatctg tttccggcca cgaactcgat tactaaactg
      961 gacgtagaga tgaaaaaaac gaatactggt ttagaggcat attattacga tcctgaaaca
     1021 ggagaagaaa tggcttctga catcatgtat gactgggaaa aattatacgc ttcagatgaa
     1081 tcggttattt atgcaggatt tgctgcagca aggaatatga cgatcaaggt agaaggaatc
     1141 gaagttgctt actcagatcc tgcgacagat cctcctgggc aagaaagacc tactcgcttg
     1201 attgatccga tttacactgt gacctctagt aatcattcag gaaaccaaga tcatactatg
     1261 tcctttagag caaatgctga cggattattg acaattaaaa ataaagcaac aggagaggtg
     1321 cttaagaacg caaaaaactt ggctatcact gcgaatcggg aatttgatta tcagacaaaa
     1381 ctgatggaag gaacgaacga atttactttc agttttacgc cagataaaaa ctatccgcca
     1441 gaagaatacg tagagatgac tagttatgaa acaaaagaga ttcttcatac agtggactac
     1501 cgtaaaccta aatattcgac agtttatgta acaccagaag ggtcagatgc aggaaacgga
     1561 agcagacaaa atccattatc tctgacacaa gcattgagat acgcatatgc tgggcagaca
     1621 atcgtcatgg cgcctggaac ttattccttc gaaagaggat tgacgattcc aaaaggcgtt
     1681 aacgggacaa gagaaaaccc gattcagttg attgcagaga aaaatccaga aaacaagcgt
     1741 cctgtacttg attttaaagg aactggaaat gggatgacac tgttcagtca ctactggggt
     1801 ttacgagggt ttgacattac aaatagtgca gctatgcaaa aaggattaca gatttctgga
     1861 aatcataatt tgatcgaaga gattcatgct tatcggaatg gaaatacagg aatccaaatc
     1921 agcggtagct caaatgatcc tttcgaagca tggcccgcgc ataacttagt caagaactgt
     1981 acatcttttg aaaatgccga tccaggattt gaagatgccg atggatttgc ggccaaactg
     2041 acaataggag aaggaaatgt atttgacggt tgtatcgcgt atcataatgc agatgacggt
     2101 tgggaccttt ttgctaaaaa tgaaacaggt tcgatcggaa aagtaatcat caaaaactca
     2161 gttgcttatc gaaacggctg ggtaccagga attgagggag aaggtaacgg taatggattc
     2221 aaaatgggcg gttcttcttt aacgggccct catgaattga tcaatagttt gtctttcgaa
     2281 aatcttgcga aaggaatcga ttccaatagc ggaacagata ttatcgtcaa tagcagtaca
     2341 agttttaata acggttcata taatgtggct ctttatacct cttcagcagg aaacacagac
     2401 taccatgctt ctggtatctt atctttccga acagagaatc ttgagatgag agagcagatc
     2461 cgcccattga atcaaaacga agatcaaatc taccactcgt tgaactacta ttggaatgaa
     2521 cgagagaaac aatcagaaaa tactcttgga cataccatat caaaagattg gatcgagtct
     2581 ctaaatacat gttcgaaaga aggtcagcag cctgttacgc gctatgaaaa tggaagtatc
     2641 catgttcatg gactgatgca gattaaaccg gggaatcgtc aaacagaaaa ggaaagagtg
     2701 gatggtcttc cattatctgt aggtgctatt tttgacggag aaacctcacc gtcttcggaa
     2761 tatccagagt atgcaatcgt acaagaacca aatcagacaa aaccaaatga acaaaataat
     2821 ccgattatta tacaagaagc aaatggtgtg gcggagaatg cagaaggaaa tatcattgaa
     2881 ctgaagcctt ttacagtttt tgtgtcaact ttcacaccaa atcaaggaac gcctgtcttt
     2941 gaagatatgc gttatttccc agaaaagatc caaatcgttg aaacacagtc aagtgctcaa
     3001 ttttctaatc ataccgctat tttttcagca ccttcaaaag gagaatatac gctagaagtc
     3061 acttatcgtt cggaaatatt tgatggggaa aaatggtctg ctcatacaga atctgttacc
     3121 cagcaaaaag cgatcactgt gcgagaaact gccgatgtgc ccccgggaaa tgacaaacca
     3181 gaaagagaaa atccaggaaa tgaatcacca aatggaaaac caaatggaaa accttctcat
     3241 ccggatagga cagcaaaaga aagtgaaaaa aatggcactc aaggactccc aaaaacggga
     3301 gaatttaata acccgttgct agcattagct ggaggaattt tgttaatcgg tgtagttgtt
     3361 tatgtcatga aacagcgaaa aaaataagtg gaatttctcc ttttatgatg taaaacaatt
     3421 gactgaggaa agtaaaaata gaaggaaatg agaccaatag atgtctcact tccttctatc
     3481 tttttataga tgaggttctc cttgctgctt ttgattggct gttcttatta cttgatgcag
     3541 gacagctttt tcacaatctc tccactaaac ggtgttcaat cagctgaccc tcggtattag
     3601 ctcaacaaac ggcaaaatat gaggaactat ttttccgttt ttttctctaa tactcaggtc
     3661 aggacgctga tttcacaacc tctcgataaa cggtgttcaa aagccagttt ctcggtagta
     3721 agtcaaattt atgcaaaaat gtggaaagcc attttcacaa aatttttctt actacttgaa
     3781 actaagcagc tttttcacaa cctctaatac atggtattcc agaagtaact tcttcgaaaa
     3841 ataagccgaa atgtcacaaa aatttgagaa gcgattttcc caaattcctt cttatttctt
     3901 gaagctgacc acttctggca caacctctta ccagattcgc actctttttt ctggcgctag
     3961 atacatagcg tcttgttccg tgataccaaa tgtttcatag aattcgtcaa ggttttttgg
     4021 ttgcacgttt gctcggagtt tagcaggggc atggacatcg atttgcagta aaagttgttg
     4081 gtattctttt tttgcttttg tacgccaaat cgttgcccaa ttgacaaaga attcctcgat
     4141 agaaagatct gattccgttt ttgctgcttc caatgcacag cttagaccgc ctgcatcagc
     4201 aatattttcc gaaacggtta attttccgtt tacttttccg tcagcaaaag gcagaccatc
     4261 gaattcttcg atcattgctt gtgctttgtt ttgaaattgt gtaagatctt cttctgtcca
     4321 ccagttgttc aagttgccat attcgtcaaa taaagcgccg ttattatcaa atgcatggga
     4381 aatctcatga gcgataacag ctccgattcc accataatta gcagagcttg attgttctag
     4441 actgtaaaat ggtgcttgaa ggatcgctgc cggaaagacg atcacattcc gaaatggatg
     4501 ataataagca ttgaccgtgt cagcactcat ttcccattct gaacgatcca ctggtttgtt
     4561 ccatttgctg aaacgatctt ttaatgtgag tttgctgaaa acaagagtat ttgaaagcaa
     4621 agtaccaccc tgatctgcag gtactgtttt gaactggtcg aataatgctg ggatcttatc
     4681 ggggtagcct acttggatac ctaagttatt taattttatg acggcttttt cttttgttgc
     4741 atccccaagc cagtcatttt cttctagacg ttttttataa acggcgatca tttttttcac
     4801 catttgttct acgtcttttt tagcagcttc gccaaaatat ttttttccgt agtaatcgcc
     4861 aactacttga tcgaattgtc cagaggcaag gtaatacgct gcttttttct gaggcattgc
     4921 ttcgtctgtt ccagacaata cgcgagaata gattccgctg acttggcgga attcttctga
     4981 tagataacca ctaagagaac gtactgtttt aatgatcatc cagcttttga ataacggaaa
     5041 atgagcttct gtcaggatgt cattcaaatg atcgaagtac actggatcag ttacgatcac
     5101 tttatctgga gttgtcccaa tcaaacccgt gacaagtttt ttcaagtcca gtttatctgt
     5161 gtatttaata aatgtatcaa aagattgcgg attatacatt ttgctgtaat ctgcgttttc
     5221 ttctgcactt tttacatgag gagcaatcaa tttatcaaat tgcactgctt gttccgtgat
     5281 tgcttctgcc tgactttttt ctttgccggc aagctctaat aactgaacag ccatatcaaa
     5341 gaaaacgcct attagttgtg caccgttggg atggtctgat gcataatacg tcttatctgg
     5401 aaggaataaa gaaggcggat aagcaaagaa ggcgttcgtt tgcgcattct tcatatctgc
     5461 atctacatcc agattaaatg gcaatggtaa tgtatccaac agccattctg gaaattgctt
     5521 attcaattcg tcgaaagact gcaatgattc aactttttcg attataggtt gaagcggttg
     5581 agcatgcaac tcatttcttc ggtcataatc gtttgccagt tgataatatg caaggaaatg
     5641 tttcattttt tctgaagaca ctttttctgg atgggcaagc agatcgtctg tttctcccat
     5701 caaaagttgg tcgatcccgt caactaaatc ttggaaaccg cctgtagctg gtttatctgc
     5761 agggattttt gctgtttttt gccattcttc attgactgct tcatagaaat cttgtttcaa
     5821 tcgttctttg ttcattcatc gatcgctcct tttttgtttc gtgtgcccat tatcttacaa
     5881 aatgaaggaa ttaacaaata gaacataaaa aattgaaaat tcgaacaatc aaactgattt
     5941 gtttttttca atccttcact tataatgaaa aaaaggaggc gatcccaact gaatcaagta
     6001 gtggataaac ctttatattt caaagtagat aaaaaaatta aaaaattagc cagcactcag
     6061 cagctgcagt caagaaaaag taaattgctt tttttagctc tggtttttga agatcaaagt
     6121 tatgtgatta tcgatcaatc aggacatccg gtagaatatt ctccagcaaa atatacttat
     6181 caagagggtc tttcttgtaa aaaatggaag ctgatcaatg aagagccaat cgagttatct
     6241 agatggatca acagaaaaga agatattccc gttttgatcg aagaaaaaag gtcaggaaag
     6301 gaactcgcaa attgctgggt gggattgcca gaagaacgtt ttttgaggta taagaaatgg
     6361 gctaccccct caggctatct ttgtggtact tacgcggcag cagttctcct tgcctattat
     6421 caagactatc gtaaggggtg gatgcttcca aatgagattc gcaagaaaaa tacggctgat
     6481 tcgttggttt tgacgaaggc attgcgtagc cagatccaac cactaggact tcctacgatt
     6541 ccttttcagg ttagtacagg gatttctagt tttttaaaga agaatggcaa tcatgagcgt
     6601 gcacgagcaa ctcttctagg ttcatggcag cgagctacaa aacgtatacg tgaaggcaaa
     6661 cctgtgatga ttggtatttt gaaagtactt ggcagtacat atggcaatca ttgggtgaca
     6721 gcgtacgctt attttgaaac tgaaacagga gaacgttatt acaaagtaca cgacaactgg
     6781 ggcgattatc ataaagtcat tcctgccagt tggagtaacg gaacagtttc acttccttaa
     6841 gaatttgctg aaattaacag aaaatcttgg aaaaatatgt taaactatcg aagaatagag
     6901 aagttttggg gaggactaca tgagtaataa gtatcaggat gacacgttga agatttttag
     6961 tttgaatggc aaccgtccat tagctgaaaa aatcgcgaaa gtgttcggaa cagaattagg
     7021 taaaagcgtc gtgaagcaat ttagtgatgg cgagatttcc atcaatatcg aagaaagtat
     7081 tcgaggggat catgtgtata tcgttcaatc aacgaatgca cctgtcaatg attactatat
     7141 ggaactactg atcatgatcg atgcaatgaa gcgagcaagt gcgaaaacga tcaatgtcgt
     7201 attgccttac tatgggtatg ctaggcaaga ccgcacggcg aaacctcatg aaccgatcac
     7261 tgcgaaattg atcgctaact tgatcgaaga agcaggggca actcgagtac tgactttaga
     7321 tctgcacacg gttcaagtac aaggattctt tgatattcct gtagataatt tatttacgat
     7381 gccgttgttt gctcattatt accgtcaatt aggtctgacg ggagatgaca tcgtagttgt
     7441 ttcgccaaaa aacagcggtg ttcagcgtgc acgtagtctt tcagaatatt tgaacagcac
     7501 attagccatc gtagatcatg cagatgatgc ttcaactgag ggtggttatg tgatcggtga
     7561 tgttcaagga aagacatgta tcatggtaga tgatatcttg aacacaggag caacgcttgc
     7621 acgagcagca aatgtattaa aagaaaacgg cgcaaaagaa gtatatgcct gtgcgtcaca
     7681 tggattattg tccgatcatg cgaaaagtat tttagacaat gcaccaatca aagatatttg
     7741 tatcactgat tctgttttca cagaagaaaa tcgtcatcca gaaaacttaa caatcgttac
     7801 atgttcagaa ttaatgggtg aagcggtcaa acgcatccat gaaaatacac cgatgagtcc
     7861 gttattccgt ctggaagaaa aagaattcga ataaacaaaa aaaagaggga aagaagttgt
     7921 gacagaagtg agcagcttca aggagctgcg acaagacttt tgtcacagct ccttttttct
     7981 atgcccatga aacgtcctct agacaaaaat gagctcttct ttggtttgtt cgctatttta
     8041 tgctagaatg tgcctagcaa taatttggaa gggatgaatg aagtgaaaac tatttatctg
     8101 gatcatgcgg caactacccc catgcatcca caagtaattg aagcgatgac ggcaatcatg
     8161 caagacacgt ttggaaatcc ttcaagcata catggatttg gtcgccacgc gcatgaaaaa
     8221 ttggaaacag caagacaaat cattgcagac agtctgcaag caaaacctca cgagatcatc
     8281 tttaacagtg ggggaacaga aggggataat acagctatct tagaaacagc gttctcgcgt
     8341 caaaaagaag gcagacatct aatcactaca gctatcgaac atcctgctgt tcttaatacc
     8401 atgaaatatt tagagacaaa ggggtttgac gttacatatc tgcctgtaga tgaaaaaggg
     8461 aatctgtcga tcgaagaggt caaaaaagcg ttacgagaag atacgatctt ggtctctgtg
     8521 atgtacggga acaatgaaat cggaaatttg atgccaatca aagaaatcgg tgaactttta
     8581 gcggatcatc cagcttattt ccatacagat gcggtacaag cgtacggaaa ccaaacgatc
     8641 catccagaag aattaggaat cgatttatta agtatctctg ctcataaaat caatgggcca
     8701 aaaggcgtcg gtttcttata taaacgagac ggagtaaata tccctgcatt gcttttaggc
     8761 ggggagcagg aagaaaagcg gcgtgcaggt acagaaaatt tagcagggat ctggggtatg
     8821 gcaaaagcag ttgaactgct gtcacctgaa gaacgtgaag cacgtaacaa caaatataaa
     8881 ggattccagc aattgattct tgatacatta gaagcaaacg aaatccctta tcaagtaaat
     8941 ggtgatttaa cgaataaact ccctcatgtg ttgaatcttc gcttaattgg cgaagcaaat
     9001 gatttgctac tgatgaagct tgatcttaat ggggtcgcta tttctactgg ttcagcttgt
     9061 acagcaggaa atatcgagcc ttcacacgta ttagaagcaa tgtatggaga agatacaccg
     9121 atactaaaag aatccatccg tatcagcttt ggtttgggaa ataccgaaga cgacatccag
     9181 tatttccttg atcaattagt tcgtgctgtc aaaaaataac tagtgaagtc attcttatat
     9241 gaacagatgt ctgagagaac gccttcattt tttagggaac ttaactgaaa aacctagtaa
     9301 aaatcaggat aaacttttat aataaagaga aactaatttt atcgaggtga aaatcatggc
     9361 atttgaaaca acagcaacgg tactgggcgc accaattagc tatcgtttgc atcctgatgc
     9421 aaaaagatat acattgcgcg ataatggatt tacagaaaca aacggaggca attatcaatt
     9481 gatccgacca ttagatgcca cgccgcaaag caaagaagga ttcaaattaa aaatcactgt
     9541 atcaaaagat attcaaacat tgaaaatgtc gatcactaca gcaaatggat tgaaagcagt
     9601 caatattttc aaagatccaa aacagaaaat gagccaagat aaattttatt ttctgatgga
     9661 tgggatgatt agccgtgggt tattcgaaaa agtcgaatga tctgtggata taaaaagaca
     9721 actagttttg aaacggccta ttgttagatg actaactttg taaggacgac gcaaattagt
     9781 tgtctttttt atttttaaat ttaacacatg ttgttatttt taatattttg ctgatttatt
     9841 gtttctaagt aaagaaattt aaattgaaac gattgttatt tagggaaaaa ggtatttttt
     9901 gaaaaaagaa tcccgtataa tgattgttgt gcaaacgcta tacaaaatga aaaagaaaag
     9961 agggatgttt atggaaaatg aacagtcggt cgttttaacg aattggaaga gaaattattt
    10021 gttcttttta tcgggacagt ttttgtcagg aatcactagt atggtcgtcc aatatgcgat
    10081 catctggtat ttgacaagag aaaccggttc ggcgacaatt ttgagttttg ctactttact
    10141 aggaatgatt cccatggttt tactaagccc atttgtagga ccgctagtgg atcgctggga
    10201 taaaaaagct cttttgattg tcacggatat tattgtagcg attttcgcgc ttattttagc
    10261 agtagtcgga acgatttcag aatccttccc gatttggcta gtttttgtgt ctttgtttat
    10321 gcgttcggtc gcacaaacat tccaaatgcc aacgatccag tcgattatgc cgacaatcgt
    10381 tccatcttct cacataacaa ggacgaatgg acagttagga atggtccaat cagcgaattt
    10441 catcattgcg ccagctctag gtgcggcttt gttttcagtc gttcctgtca gttaccttat
    10501 tttattagat gtattggggg cagtttttgg tgtcggctta ttgatttttg tgaagatccc
    10561 aaaagtttct cctgaaatat tggaagttcc gcttactatt tttaaagatg caaaatttgg
    10621 gctgcagcaa ttgatggata ataaagggtt atggtacata acgatcaatg gtgcatttgt
    10681 gatgctgttg ttcatgccag caatcagtct gtatccattg atgacgctag attactttgg
    10741 tggttccgtt ggacaggcgg gagcagtgga agtagtctac gcagtcggaa tgctcttagg
    10801 cggtgcactg atcagtttta taggaacatg gaaagaccga atgaaaccta ttattatagc
    10861 ctacatcatt atgggcctga cgatcggtgc aagcggattg gttccaaacg atagtcaagg
    10921 gttcttgtac tttcttatct tgaatgcagg agcaggttgt gcaacgccat atttcaatac
    10981 actgctgatg gcaatgatcc agcagagtta tgaatcgaat gttttaggtc gtgtcttagg
    11041 gaacttcaac tctttgatga acctggcagg tccaattgga ttattatttg caggaccatt
    11101 agctgatcgt ttaggggtag aaaaaatgtt cttgttttcc ggaatcggga ttttattatg
    11161 cggaatcgtt ttatttttaa cgtcagctgc ccgaaattat gataaagaat tacagaaaaa
    11221 actggtaaaa gaacaccatg agcaaaaaga cgaatagtat ggaatggagt tgtgacagaa
    11281 gtcttgttac aactccattt ctgatcctaa tgttcctgtg gcaataagcc tactcaaatg
    11341 aaaaatatga gtaattatct ttatttgttt aatacttgat atagggcagt tttttcacga
    11401 cctatcgtac ttcctctttg cttcatagat ttctttgatt tgttgctcag ataacggagt
    11461 gccaaatggt agtttttcct ttaagagttc tctttccggt gcgtctacgc ctacattgac
    11521 atcgttctcg ataggtacgc tgtaatgatg gatatgtcca tgaagattgc ggatagtgtc
    11581 gttaggcccg agcaacatcg gataatgagt caagtaatat tgttgatggt cgaatttaac
    11641 gatggcacct acatcataaa aataaaattt gggacttccg tcagtaagcc taggatcatt
    11701 tttttctaaa taacggaaaa aagcgcgact gtcatggttc cctttgatca agtggattgt
    11761 tccttgcagt tgagtgacca gttccaaaat ttctgcattt gctttttcat attgcggatg
    11821 catagcaagg tcgcctagat gataaacatg gtcggtttct tttattactg cattccagct
    11881 agtgatcaac tgttggttca tttcatccac atttgcaaag agccgcggag caaagtcgtt
    11941 ttttcctaat aagcgttcat ggaaaaagtg catatcactg ataaagtaat tcatgtatct
    12001 tttgcctccc ttttttctta gagtaaacaa aatcacttat ctgagcaact cataacatct
    12061 aaaaagaaaa ggttgtaaat gagacgtgca gctttaagcg ataagaagca attttgaaaa
    12121 atagtttttc acaatttata aaattattcc ttattgccga aaggctggcc ctttttatga
    12181 gtatgccaat tttttatatc gaatgctcaa aagatgagtg attttttggt tgactatcat
    12241 gtttgtgtac tgaataggtc cagtagaaca gtatttttac gacttgataa ttaagggatg
    12301 aaattttcag aaatatgacg tataatgtaa aatactgaaa agttgcgacc ttcaactcgt
    12361 agatgtttca gaatctaatt gaaatgatgg tgatatcatg acagacaaca gcaaaacacg
    12421 cgttgtcgta gggatgagcg gcggtgtcga ctcatctgta acagcacttt tgttaaagga
    12481 acaaggatac gacgtgatcg gaatattcat gaaaaattgg gatgacacgg acgaaaacgg
    12541 tgtatgcacg gccacggaag attataagga tgtagcgaaa gtagctgctc aaatcgggat
    12601 tccttattac tctgtgaatt ttgaaaaaga atattgggat cgtgtatttg aatatttcct
    12661 agccgaatac cgcgcaggac gtacacctaa cccagacgtg atgtgcaaca aagaaatcaa
    12721 attcaaagca ttcttggatt atgcaatgga tcttggcgcc gattatgtag ctactggaca
    12781 ttatgcgcaa gtcactcgcg atgaaaacgg cgtggtccac atgcttcgtg gagtagacaa
    12841 taataaagat caaacttact tccttagcca attatcacaa gaacagcttt caaaaacgat
    12901 gttccctcta ggcggcatgg aaaaatcaga agtacgggca attgctgaac gcgcaggcct
    12961 tgcgactgct aagaaaaaag attcaacggg aatctgcttt attggtgaaa agaacttcaa
    13021 acaatttttg agcaactatc tgccagcaaa aaaaggaaat atggtcactc ttgatggtga
    13081 agtcaaagga caacatgctg gcttgatgta ttatacgatt ggtcaacgtc aaggactagg
    13141 gatcggtggc ggaggagatt cgcaagaacc ctggtttgtt gtaggaaaag atctagcgac
    13201 aaacacatta tatgtggggc aagggttcca tcatcctgca ttgtatgcca caagcttgga
    13261 tgcaagtgaa atccacttta cgacgaatga accaatgcca aaagaattca agtgtacagc
    13321 gaaattccgt tatcgtcagc aagacgttcc tgtgacagtt cgcttattgg atgataatcg
    13381 tgcagaagtc gtattcgacg aacctgtacg tgcaatcact cctggacaag cagttgtatt
    13441 ttatgatgga atggaatgtc tgggtggtgg tttgatcgac cacgcatatc aagaaacaaa
    13501 agttttacaa tacgtttaag cgatattaaa tgaatcaaaa cagctatggc taacaaaaga
    13561 aacacctttt cttttggata gcatagctgt ttttttgcaa taaaattaat ttttattcat
    13621 tttatacttg aaatacttgt gtaaaaagtt cataatatcc aataatatga ataaagttgc
    13681 tttttgttaa gcaagagcag ttgcaaaata gaagggaatc ttttgcaagt gatttgttca
    13741 attttataag tgagggtgat ggaaatggaa aagaaatcga cgggtattag caaatgggga
    13801 ctggttgctc tggttgtcag ttcgtcaatc ggaagcggtg tgtttgggat cacaagtgat
    13861 ctggctagtt ctgcagctcc agggccagcg atcctttcat ggataatcgt tgggattggt
    13921 attttagctt tggtattatc gctgaatcat ttaggagaaa agcgtccaga tctagatggt
    13981 ggtattttcg gttacgcgga agcttcattt ggtaaattag gtggcttcat cagtggttgg
    14041 ggttactggt tgtctgcgtg gctgggaaat gttgcttttg caacgatgct gatgagcgcc
    14101 atgggagaat ttttcccgat attcaaaggt ggacaaaatg ttccttctat tttatttgca
    14161 agtattttta tttggggtct gactttatta gtaaacaatg gagttgaagg agcgagcgtc
    14221 atcaatacaa tcgtgacgat ctgtaaatta gtgccattat tcctattttt aatatttgta
    14281 tttatcgcat ttaaagtgaa tatattcact gctgattttt ggggtaacgt atctgataat
    14341 ctagtgaatg gaacagggca gcaaggaacg atctggttgc agattaaagg ttgtttgatg
    14401 gttatgatgt gggtctttgt agggatcgaa ggcgcaagtg ttttagccaa ccgcgcgaag
    14461 aaacgttcag aagcccagca agcatcgatc attggtctgc tttgtttatt gtttatctat
    14521 atccttgctt cgatcttgcc atacggcgtc ttatctcaag aagagttagc aactatttca
    14581 caacctgcta tggcaaatat tttaaaagga atcgtgggaa attggggagc agcgctgatc
    14641 aatatcggct tgattatttc gatcattggt tcttggttgt cttggacgat gctcccagca
    14701 gaaacaacga tgttgatggc tcgagacggt ctgttgccga aaggttgggg aaaaacgaat
    14761 aaaaaaaatg ccccaactta ttcgttagtt gttactgcaa tcttgactaa tctattctta
    14821 ttgactttct tagttactga ttatgcctat caatttgcct attctttatg tacagcggca
    14881 attttgattt gttatctact tgtcgggatc tatcaagtcc agttttcttt aaaagaagga
    14941 atcaaaaaac aagtagtgat cggtgggatt gctgtagttt ttgaattgat gggcatcatt
    15001 ttagcaggat tttcttatgt ccttttatgt agtattgcct atcttcctgg tttttgcttc
    15061 tactggatcg cttgtcgtga aaccaaacat caaattacgg gaaaagaaaa agtaatgatt
    15121 gggctgattt gtgtggccgc actgctttca atcgggttgc ttgctgtagg ctggatcaag
    15181 atataagggg gaaagaaaca tgaaaatcgc aaatttcggt gtagaagaat ggctaaataa
    15241 atgggaaaag aaagcggtat atgatattgc acaaagctcg atcgaagcat tgacattaga
    15301 cgaactcgtt ggcttagatg ggactagcgt aagtaaattt ttcgaaaaac gcaaaaacga
    15361 accattggac tatggctgga tcgaaggatc agatgttttc aaacaagaag tcgcttctct
    15421 ttatcaaacc gtcgatccgg aaaacatctt acaaacgaat ggagcgactg gtgctaatct
    15481 tttagctcta tacgcgttag tggaagcagg agaccatgtt gtttcgatgc ttcctactta
    15541 tcagcaatta tatgacatcc ccaaatcttt aggtgccgct gttgactttg ttcatttgaa
    15601 ggaagaagaa gattggcagt ttgatttaga acagctagaa gccttagtca aacctgaaac
    15661 aaaaatcatc tgtttgaata gtgcaaacaa tcctacaggc acattgctag atcggaaaac
    15721 tttagaaaaa atcgcagaaa ttgctcgtac agtagatgca tatgtattga tagatgaagt
    15781 atatgcgcct ttaacggata aaggtgaatt cttgtcgatc gtagatgtct atgaaaaagg
    15841 tatagcaacc aattccctat ccaaaaccta ctccattcca ggtattcgta ttggctggac
    15901 agctactgga cctgagttgg cagaagtttt tcgaaagtat cgagactaca cgatgatttg
    15961 cggcggagtc ctgtcagatg atcttgctgt acatgcattg aagaataaag aaaaaatatt
    16021 ggcgcgaaac aaaaagatca ttacagagaa ccttgtgatt ttaaaacaat gggtagccaa
    16081 tgaaccgaaa gttgaacttg tcgctcccaa ttacgtctct acttctttca ttaaattagc
    16141 catcgaagaa gatgaccaga cattttgtat caatttatta gaagaaacag gagtactatt
    16201 agtaccagga agtgcctttg atttaccgaa gcatgcccga ctaggttact gttgtaaaaa
    16261 agaaaccttg gaaaagggat tattcctctt atctgagttc ttgaaaaaac aggcctaaac
    16321 aaaaacactg caaggacagt ctgtgacaac tgttcaagca gtgtttttca tttacgctta
    16381 ttgtctatga gaaggagagt gccctgaagc tatggagact cctgaagctt tttcttctgc
    16441 agtttcttgc tgatggatct cttgtgtatt gtgtcgtaca agcaagaagt tgaccaaaac
    16501 aagaagagaa gtcgaaagaa agacgccacg ataaccaaat gcactggaaa cactggagcc
    16561 gatcataggc cctactacgt taccagttgc ttggaaagat tggttataac tgaagatccg
    16621 tccagcagct tgatgaggcg aatattttgt gatcaatgct tggacagcag ggagcagaca
    16681 agcatcagag ataccaatca aaaaacgtaa ggcagctaat tgccaaacat tcgtgacgaa
    16741 tgccattggg atataaacca gaatcgcaaa acccaaacca atcgcaagta ttctttcact
    16801 cccaatcttg tcacctaagc gaccaaatct tggagcggct atcaaagtgg caatcccagg
    16861 tatcgaagca atgattccgc tgatcaacgt aacattccca tgtccatgca gcaattggcg
    16921 aatgtacaga ctgatgattg gactaatgga attgttggat gcttggatga tcatcgttgt
    16981 gacgaacata ccgatgacga cgtgtggata tttcaagtct ttgaaaatct gtttggcaga
    17041 aaccatgttt tcttttttca ctgggacaaa tttttcatgg acgaaaaaca gactcaacaa
    17101 gaagacgaaa aataagatga tccctgtaat gaaaaaggta gggcgatagc caaacgctga
    17161 agcagagatc ccgccaagaa gcggtccaag taatgttcct gtaacagaac cagttgctaa
    17221 ggttccaaga acttgaccac ttttttcttt tggcgtccca gttgcgacaa gggcagtggc
    17281 attactgata taaccagaaa aaatcccttg aagcagacgc aatgcgacta attgatacac
    17341 actagtcacg caacccatca aagaaatgac gattgccatt cctaatgaag cacgcaacag
    17401 catcaatttt cttccttttt ggtcggctaa ttttccccac caaggagaga taagcgtagt
    17461 caccaagaaa gtagatgaaa aagtcaatcc gctccaaaag ttcaattgtg acgtactata
    17521 attacctaac gtatctatgt aaagtgacat aaaaggcatg actaaactaa atccgatacc
    17581 agccataaat gttccaaacc ataagacaaa caagttttgt tcccaagttt ctcttttcct
    17641 aaataatttt tcctttattc ttgcagccaa ttagactcct tctttctgaa aattttcatc
    17701 tacagctctt tattataaac tacaatatgt agtttatcaa tgaaagtctc ttttttctct
    17761 ttctttttcg tggtaaactg aaagagtgaa ggatttattt tatgagagga aacatggaaa
    17821 tgaaaaaaag aaatctgtct caagagaaaa tcatcgactg ctttcgagaa ctagcagaag
    17881 agatggatgt tcagcaaata acctttcagc atttagccaa acgcttggat atcaaggctc
    17941 cctcgctgta taaccatttc aaaaatattc gtgatgtcaa aacagcgcta acagcgaaac
    18001 ttctgcagga acttaatgac cagctgcgac gggctcttgt gggaaaaagt ggtcaggaag
    18061 cactgcagat ctatgcagaa acttatcgat cctttgcttt ttcaaatcaa gcagtgtatg
    18121 agttactgat cagtgtaccg cataccaatg aagctgtttt attagatgga atccatgaaa
    18181 cgaatcagat cattttgcag atatttgagg cttttcaatt aacgaaagaa gaaaaagttc
    18241 atcgaagcag ggaacttcgt agtatgatcc acggttatct ttccttacgt tttttaggat
    18301 actttacaaa agaaccaaca atcgatccag aagacagcta tttttggatg atcaataact
    18361 ttatcgctac tttgccttaa acaatcagat tcatttgtaa caattttaag atgttcatta
    18421 aatgaccctt ttataagaga aaattcttgc aactttcttg aaaagtgact aagatgagtc
    18481 tagaaaagct gaaagaagga gcacgagtat gtatcaagta attaccatgt tcggggacaa
    18541 tgagccttgg tggttttttg aagactggga agaagatatt gaagaagaag aaacattcga
    18601 gtctttcgaa gaagcacgac aagcgtacga aaaacaatgg aacgaaatcc atcaaaatta
    18661 tgaatatatc aatgcaaaaa gtaactttct cagcgccttt tggaatgacg gagatgagcg
    18721 ctggtgtgaa gagtgtgacg atgatttaca gcaatacaag ggacttgcgt tgttgaaaaa
    18781 tcatcagccc atcacagtag aaagcagaaa ggaattttat gaaacaacta attctagcgg
    18841 aaaaaccaag cgttgcgaaa gacctaagca aggtgcttgg tgctaatcaa aagcacaaaa
    18901 attattatga aggacccaag gtgatcgtta cttgggccct tggtcattta ttaggattga
    18961 aaatgccgga agatctgaat aaggaatggc agagctggca aatggacacg ttgccaatga
    19021 tccctaaaaa actaggaatc aaaccacttc ctaaaacagg acatcaatta aaagcaatca
    19081 agcagctggc tcagcgaaaa gatgtctcag aagcagtcat tgcgacagat gccggaagag
    19141 aaggggaact tgttgctcgc tggatcttag agtatgtgca tttcaataaa ccagtgaagc
    19201 ggttatggat ctcttctcaa acagataaag caatcaaaac tggattcaag caactaagac
    19261 ctgcgaaaga atatgatgca ttgtatgaat ctgctcttgc ccgggcaaaa gcggactggc
    19321 tggtaggatt gaatgtgacc agagccttaa ctgtgaagta cgcggataat ttatcagcag
    19381 gccgtgtgca gacaccgaca ctagcattag tgagagacca agaaaagaaa atcgagcagt
    19441 tccgtccaca aacgtatttc acgatcacgt tgcaagctgg aaaagaacaa gccaaaatgc
    19501 ttcaaaaaaa tcaatttgct ttaaaaacac aagaagaagc acaaaaactt gttcaattga
    19561 tcagtacaag caaaggaact gtcacagaag tccaagaaaa attaaaaacg gaatctgcgc
    19621 cattgcctta tgatttaaca gagatccaaa gagaagccaa tcaacgctat ggctattctg
    19681 caaagaaaac attaagtctt gtacaaagtt tgtatgagac ccataagatc gttacttatc
    19741 cgcgtacaga cagcaaatat ctgacaaatg acatgaaaag tacgatgaaa gaacgattgc
    19801 aagcagtcag cgactttgct cctgaggtca agcagtattt gaaaaacgga gccatcgtcc
    19861 aacagacaaa agtattccaa gacagtaaag tgaccgatca ccatgcactt ttgccaactg
    19921 aaaacagagc acgttatgaa aaattaagta atgaagagcg aaaaatctat caaatgattg
    19981 tctctcgttt tttaggactg tttgctcaac cccataaagt cagccaaaca aaagttacgg
    20041 ttgaatttgg taaagaacga ttcatttttc ggcaaaatcg tgtcgtccaa gcaggatgga
    20101 aaggcgaaac tgaaactgag acggaaacag tcaaatggga aaaaggaatg agaatcaatc
    20161 ccgatttcgc catcaaaaaa gaactatctg caccgcctaa accattgtca gaagcaagct
    20221 tgcttggtca aatggaaaaa tacagtctgg gaaccccagc tacgcgtgca gagattatcg
    20281 agaaactaat caaatctgaa ctgatggaac gtacccctca aggattgcaa gtatctccta
    20341 aaggtaagca attattagta ttggtaaatc cctctttagt aacaccagat ttgacagaaa
    20401 gctgggaaaa agaccttgaa gctattgctg caggaaagaa acaagcagga accttcttga
    20461 atggaatcga aaaagaaaca aaacgcttgg tgaatgagat caaatcaagc aaacaagaat
    20521 atcaagattt ctcgattacc caaaagaaat gcccagaatg tggggccaat ctgcgcgaga
    20581 aaaacacacg tgacggcaaa atctatgtct gcaccaattc agattgcaac taccgcagaa
    20641 gaaaagatcc aaaagtctct aatcatcgat gcccgcaatg tcaccggaaa atggaaatca
    20701 tcgaaggtaa aaacggtgct tatttccgtt gtaaatacga tggaacgaca gaaaaattgc
    20761 ttgataaaaa agagcagaag aagaaaatga ccaaacatga agaacgacgc ttgctgaaaa
    20821 aatatgcaca agcagaagaa ccagaagaaa gcccattagc agcagcacta aaagcagcaa
    20881 tgaagaagta ataagatatg aaagaggttg tgatagaagt ggtcagcttc aagaaataag
    20941 aaggaattta cgaaaattgc ttctcaaatt tttgtgaaat ttcggcttat ttccgaagaa
    21001 gctacttctg gaacaccatt tattcggaaa aaaggactgt gacaagactt ttgtcacagt
    21061 cctttttcat ttaaaaaaat gtttacttca tttgacgtat attattggtg aggcttcaat
    21121 tcatttggct tcactttttc tgtttctctt atgatataaa caggacgctg tttcgtttct
    21181 aagaagattt tcccaatata tttcccgatg attccaagac ataacaattg gattccacca
    21241 atgaacagga agatcgagac catggaaggc catcctgatg taggatcacc aaacaacaat
    21301 gcccgcaaaa taatgaacaa aattgcaata ccagaaccga cacaagacaa agcgccgata
    21361 aatgaagcaa tcgttaacgg tgcatccgag aagttcacga taccatcaat cgagtagttg
    21421 aataatttcc agaaggacca agatgtttcg ccagccacac gttcgcggtt ttcataagaa
    21481 atatattctg ttttaaatcc gacccagcta aataatcctt ttgaaaatcg gttgtattct
    21541 gttaattcta ggacggcatc caccatttga cgagtcatca agcggaaatc acgagcgcca
    21601 tcgaccattt ctgtatcgct gattttattg atcaactggt agaatttttt cgcaaagaaa
    21661 ctgcgtatcg caggctcgcc ttcacgagtc gaacgccggg tgccgacaca atcgatatca
    21721 gaagtagtga tccggttgat catctcagga agcagttcgg gcggatcttg cagatcaaca
    21781 tccatcacgg tcactaaatc tcctgtagaa gcttgcaatc cagcgtacag agccgcttct
    21841 ttcccaaaat ttctggaaaa tgagacaaag cggacataag gataagctga ggctagcttc
    21901 ttgattgctg ctaacgtacc gtcttttgat ccgtcgtcaa taaacaaata ttcgaactca
    21961 tgggcacctt tgaatttttc aacctcatca aaaaaaagag gaacagtttt ttcttcatta
    22021 aaacatggga caacaattga tactttcatc gtttctatcc tttcaatcgt atgtttatat
    22081 atggtttaca ttcaaaattt aataaacctt aaataacacc tttaaaattt gattttacgt
    22141 aaaagaacag gtattctttt tgtaaactaa cgtatttagt ttaccatgtt tcctcggttt
    22201 atcaaacttt taagaacaat ttttattagt tggcaagtag ttttggatag agaaaaagga
    22261 ggaagttcca gtctattgac taggagcaaa caaaatgaaa atacacacac ctatcggctt
    22321 tttatggtta gatgcagatg aatccggatt gactgctgta tcttttcagc caatttcaga
    22381 aagcgaatca gcgaatcagc gaattttaac gcgcacacaa gaacagtttg aagaatattt
    22441 tgcgggaaaa agaaaagaat ttgatgttcc tttatctata aagagaggta cgccttttca
    22501 gcaaaatgtc tggaaagctt tgtcgaccat tccttatgga gaaatccgaa cgtataaaga
    22561 agtagccgtt gctgttgata gtccgaaagc tgtgcgagcg attgggcagg caaatcgaac
    22621 caatccattg ccaatcgtta ttccttgtca tcgagtgatc gggcaaaatg gaaaactgac
    22681 aggatatatg ggaaacgcaa tagatggggt tgaaatcaaa cgccaattgt tgttgttaga
    22741 aggatatctg caaaa
//
LOCUS       DAXWOO010000033        21835 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.033, whole genome shotgun sequence.
ACCESSION   DAXWOO010000033 DAXWOO010000000
VERSION     DAXWOO010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21835
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.033"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(265..378)
                     /locus_tag="ACGE70_000982"
                     /pseudo
     CDS             complement(265..378)
                     /locus_tag="ACGE70_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(761..1993)
                     /locus_tag="ACGE70_000983"
     CDS             complement(761..1993)
                     /locus_tag="ACGE70_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFF8692633.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            2247..2816
                     /locus_tag="ACGE70_000984"
     CDS             2247..2816
                     /locus_tag="ACGE70_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFF8692634.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2994..3434)
                     /locus_tag="ACGE70_000985"
     CDS             complement(2994..3434)
                     /locus_tag="ACGE70_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFF8692635.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            3592..4356
                     /gene="map"
                     /locus_tag="ACGE70_000986"
     CDS             3592..4356
                     /gene="map"
                     /locus_tag="ACGE70_000986"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFF8692636.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            4388..5311
                     /locus_tag="ACGE70_000987"
     CDS             4388..5311
                     /locus_tag="ACGE70_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFF8692637.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIVPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            5387..6526
                     /locus_tag="ACGE70_000988"
     CDS             5387..6526
                     /locus_tag="ACGE70_000988"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFF8692638.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            6519..7319
                     /locus_tag="ACGE70_000989"
     CDS             6519..7319
                     /locus_tag="ACGE70_000989"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFF8692639.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            7319..8146
                     /locus_tag="ACGE70_000990"
     CDS             7319..8146
                     /locus_tag="ACGE70_000990"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFF8692640.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            8151..8858
                     /locus_tag="ACGE70_000991"
     CDS             8151..8858
                     /locus_tag="ACGE70_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFF8692641.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            8958..9824
                     /gene="rfbA"
                     /locus_tag="ACGE70_000992"
     CDS             8958..9824
                     /gene="rfbA"
                     /locus_tag="ACGE70_000992"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFF8692642.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9838..10410
                     /gene="rfbC"
                     /locus_tag="ACGE70_000993"
     CDS             9838..10410
                     /gene="rfbC"
                     /locus_tag="ACGE70_000993"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFF8692643.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            10432..11460
                     /gene="rfbB"
                     /locus_tag="ACGE70_000994"
     CDS             10432..11460
                     /gene="rfbB"
                     /locus_tag="ACGE70_000994"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFF8692644.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            11558..12409
                     /gene="rfbD"
                     /locus_tag="ACGE70_000995"
     CDS             11558..12409
                     /gene="rfbD"
                     /locus_tag="ACGE70_000995"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFF8692645.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            12444..14477
                     /locus_tag="ACGE70_000996"
     CDS             12444..14477
                     /locus_tag="ACGE70_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692646.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKQPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            14521..15801
                     /locus_tag="ACGE70_000997"
     CDS             14521..15801
                     /locus_tag="ACGE70_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFF8692647.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVTEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            16011..16817
                     /locus_tag="ACGE70_000998"
     CDS             16011..16817
                     /locus_tag="ACGE70_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8692648.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16829..18049
                     /locus_tag="ACGE70_000999"
     CDS             16829..18049
                     /locus_tag="ACGE70_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8692649.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGKVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            18039..19625
                     /locus_tag="ACGE70_001000"
     CDS             18039..19625
                     /locus_tag="ACGE70_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFF8692650.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLYINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAQPQNSNYVKVLEVIQQTKQDEIIQQIPFNNFTGENETLSFHVSELA
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKDLPEFFLTDIAG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHYLIENNTRLD
                     QTLKTTINRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSLCIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVLREEVNQAKQLAEGAKAGFELRILCEK
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19664..21802
                     /locus_tag="ACGE70_001001"
     CDS             19664..21802
                     /locus_tag="ACGE70_001001"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFF8692651.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEVQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALKMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPAEHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVHPGRISGFYEITYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     ILEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFSLRPD"
BASE COUNT         6888 a         3701 c         4541 g         6705 t
ORIGIN      
        1 tcagcataaa agttcacctc aaaaagagta tacgaacgaa tgttcttttt gtgaaggcgg
       61 aatttaatat actgaataat agaaaacaag tacttgtgcc aaattatgtg ccaaaaaaat
      121 tcgaattcaa caaaatgtaa acaaatagaa aaccttctat attaagattt cctgcaataa
      181 caaacacaac agaagacgtg taatagttag tcaggaacgt acaaataacc cctgtatcct
      241 ttgcggtaca gggggttatt ttgtataagt agacattgta gtttctaaac tgctatgtcc
      301 taaacgtttc gatacgctaa gtatatttac tccttgataa agtaaaatag atgcatgcgt
      361 atgtcttagt ccatcaataa acagtcgaag agaaaatagg gaaaaattga ttactaaaga
      421 aaaacttact aggtcgacgg tgtagttgat tttcatactt caatagattc gaattaaacg
      481 atttcgattg gcttatacca gtcctatcca acgaaagata tatcgttctt aactctagcg
      541 gtataacttg aagctttacg taaagccaat gggaatgaac tggagatgac tttttattat
      601 ttttagtaat agctgatggg ttaatactta caactttcct tttcaaagta ccatataaaa
      661 aaacggtggg gttgtttagc gaaataaagg atctaagcca atagcttaga tcctttattc
      721 tatgctttat attatttttt gcatgtgtgc gaaagtatac tcaagcccaa tcgccatttc
      781 ggaaaatcgg tactgtagag ccatcttcac ggattccgtc gatatccatc ttgtcagatc
      841 ctaccatgaa atctacgtgg gtttgacttc tattcaatcc agcttctgct aattcttcat
      901 cggacatttc tgtaccgcct ttcacactga aggcataagc agaacctagt gctaagtgat
      961 tcgatgcgtt ttcatcaaat aatgtattga aaaagatgat gccagattga gaaataggtg
     1021 aagggtcagg tacaagtgcg acttctccta aacgacgcgc accttcatct gtatcaagca
     1081 gttttgctaa gacatcttct ccttgttcgg cagaaaaatc aacaactttt ccatctttaa
     1141 aagtaaattt catacccgag atgatggtgc cagcataact cagtggcttt gtacttgaaa
     1201 tgtaaccatc tactcgatga ctgtcaggtg ctgtgaacac ttcttccgtc ggcatatttg
     1261 ccatgaattt ttctccacga gcattatagc ttccagcgcc ttcccaaaga tggtttttag
     1321 gcaatccgat aatgatatct gtaccgggag cagtatagtg aagggcagaa aattgttctc
     1381 gattcaattc ttctgctttt tttgctaatt tttcatcatg cttcttccat gctaagacag
     1441 ggtcttcttc atagacacga gtcgttttga aaatctgatc ccataatgcc tcaacttgtt
     1501 tttcttctgg cagttctggg aagacttttg ctgcccattg tttaccagcc gcagccacga
     1561 cagtccaact gactttattt gcttgagttg ccttgcgtaa attcattagt gctttgccat
     1621 tcgctgattg ataagacgct actcgatggc tatctacacc agcaaatgca tcgggatcgg
     1681 cagaaacaac gctgatcctg cttgctcctt tttcaagcca ttcatccgct tgatcgattt
     1741 tagattgtgg tacattctca atacgatccg ttgcagcatg taaaagaaat tctctttgga
     1801 tctggtcgtc agtccattga acgataacct cagcggctcc tagtttataa gcttcttgtg
     1861 tgatcaatcg agcaaggggt gcctgttcca cgctgatctg taaaacaact gtgtgtcctt
     1921 tttctgtggc tacgccagtt tctgcaatta agcgtgcata tttttttaga agttcattaa
     1981 aatcagataa cataataaaa attcctcctc accatttttt ctggtaacta ttagcatgat
     2041 accatttaat tatgattcaa acaatgaggc tttaaataaa aaaaggaggt tattttttta
     2101 aggttaagtt tcatgatata tgttactact tgctatttca gcttgtgaag aaaatcaaaa
     2161 actaattttg catatcaaaa aaacatgtta caatgaaatt aatcaatggt ggaagcgtta
     2221 taaaaaaatt aaggagatta aaaaatatgg caagaaaaaa gacgattact agagaacaga
     2281 ttttggcagc tgcttatgaa gtagctgcca ccgatggatt tacacgattt actgcgagaa
     2341 acatcgcgaa caaaatgaat tgttccacgc aaccgattta tcttgagttt aaaaatatgg
     2401 atgacttgag acaagctctt tttgagcaga tctatgatca tttacaatat gaagtgttca
     2461 ctgttgagca tacagggaat acgattatcg atcttgcttt taattatatc cattttgcaa
     2521 gaagagagaa gaagttgtat cgttcactgt acttagaaga atatggtgga ggaaaagaga
     2581 tgcagaattt ctcttatact tactttgtga atacagtgaa acgcgatcca gcttatgtga
     2641 atctttcaga agagcagatc gaatctttgt ataatgggac atggatcata gctactggga
     2701 tcgcagcttt gatgtcttca agcatcatcc atccaactga tgaacagatt gaaaagatga
     2761 tccaagacag tatagatgct atcttgaaat tcaaagaacc aatcgaaatc gattgaatat
     2821 actcaaaagt tccacaaatc agtaggtaac aagagatgtg tcaaaataag gaaaagttcg
     2881 tttttcttac agcgtatgca tttttaactc ccgaagaact cttccatgaa tgcaatgaat
     2941 aaaagagcca tacagattga ataaatctgg atggctcttt tattgcaagt atattaaatt
     3001 tttgcgacta gttctttagc gaaagcttct aaattttcga tatcttcttc ttcagcattc
     3061 agatccactt taacattttc ggctcctttt gatgcgcctg tttttgcaaa agcagcatcg
     3121 aaatcatcca ccgatttgca gaattcatcg taaaaagtat caccagatcc gcagacaccg
     3181 taaatttttc ccaatagatc caattcttgc aagtcttcat aaaagtctac gatctcatca
     3241 ggcagatcgc catcatcgta tgtataagta gctactacgc aaatatctgc ctcctcgaat
     3301 tcatctgcat ctacttgggt acattcgttg atttcgactt cgatatccag attttcaaat
     3361 gcttctgcaa caatatcggc gatttcttca gtgtttcctg tcatgctagc atatacgatt
     3421 tttgctaaag ccatgatgtg cctcctcgtt tataattatt taacaattat gaattatacc
     3481 aaaacatcat tgaaaagaaa ataaaaccta aagacacctg gggaatgtca tttggctaaa
     3541 tgtatgccgc tatgttaaaa tgagagggat ttgtaaaaag gagacgaaaa tatgattact
     3601 ttaaaatcag cacgtgaaat tgaaatgatg gcagaatctg gagctttatt agcagatgta
     3661 cataaaaatc tgcgtgattt tataaaacca ggaatcacaa gttgggatat tgaggtattt
     3721 gtcagaaact atatcgagag tcatggcgga attgctgcgc aaattggctt tgaagggtat
     3781 gaatatgcga cttgtatcag tataaatgat gaaatttgtc atggattccc tcgtaaaaag
     3841 cctttgaaaa acggtgattt ggtaaaagtc gatatgtgta tcgatttgaa aggcggcatt
     3901 tctgattctt gttgggccta tgtagttgga gaatctacac ctgagatcga ccgattgatg
     3961 gaagtaacga agaaagcttt gtacatcggt attgagcaag cacaagtagg gaatcggatt
     4021 ggtgacatcg gtcatgctat ccaaacttat gtagaaggcg aaaatctagc gatcgttcgt
     4081 gattttattg gtcatggcgt tggtcctacg attcatgaag aaccagcagt tcctcattac
     4141 ggagaagctg gaaaagggtt gcggttgaaa gaaggcatgg ttatcacgat cgaacctatg
     4201 gttaataccg gaacttggaa aatgaaaatg gatcctaacg gctggactgc gtatacaaga
     4261 gacggcggac tttcttgcca atatgaacat acattggcta ttactaaaga tggtccaaga
     4321 attttgacat cacaaggaga agaaggcact tattgatcta tccagtcaat aggtacggga
     4381 gaggtacatg aaagtttgga acaaaataaa acaaaatgaa aatctgatgc gctttattga
     4441 gacgactcaa aagcgcatca ctgattcaga aatgagtacg acctctgtcg tcgttgctta
     4501 ttatttactc ttgtctttat ttccattgat tattgctttt ggtaatattt taccgtttct
     4561 ccacatcgat caagagactg tactgaccta tttacgagaa gtgattcctg agaccgttta
     4621 ccagtttatc gttcctgcaa tcaaagacct tttgacacaa agttcaggag gcttgttatc
     4681 cgtttctgca ttagcggctt tatggtctgc aagtcaaagt atcaatgctt tgcaaattgc
     4741 gatgaataaa gcgtatggcg tagaaaatag aaaaaatttt ttgatcgtcc gtttcttctc
     4801 actaattgtc attgtcttgt tcatgattgc tattagtggg gtaactatcg ttcttgggtt
     4861 gggacagctg attttggatg ctattcagcc aatcgtacaa atacccgtga actttatcga
     4921 tcagttccaa actttgaagt ggccaatcac attggtagct ttatttgtca tcatgttctt
     4981 gatttattta attgtcccta atgctcagtt gaagttgaag acgatcattc cgggcacgat
     5041 cttcgctact gtcggctgga tgctgttatc acaagtgttt agtatctatg ctaggtattt
     5101 tgcaacgagg atcagcggtt atcagatcat aggaagtttt attgtactca tgctttggct
     5161 taattttgct gctacgatca tcattttggg cggaatcatc aatgcagttg ttcaagaata
     5221 cgtgacgggt catcaaatca aaaaacgtcg agatccaagc cgaaaatggt tcaataaatt
     5281 gaaaactaaa ttttctcata aaaaaaagta gaaacaagag ttcttattgt aaatttatat
     5341 cagtaattta taataattat gactcttaat taaagtaggt gcccatatgc ttgtttcttt
     5401 tagtatggtt ataagatttt ttctgacaat tttattatca ctgatattaa cacctgtatt
     5461 caaaatcatt tcagtacaaa caggtatggt cgacaaacca aatgaaagaa gaataaataa
     5521 agttccaatg ccttcagcgg gcggactgcc tatctttatt tcatttgtta tatcgacttt
     5581 attcttattc cgtaaaatta tccctcaatc gtatattgta ccgatcttga ttgcttcttt
     5641 agtaatcata gtcacaggag tgctcgatga taaatatgag ctgacaccta aacaaaaaag
     5701 tatagggatc ttgattgctt ctcttattat ttattttgta gcagatatac gtattgattc
     5761 cttcacgctt ccttttattg gatatatcca actaggctgg cttagttttc ccgtaacgat
     5821 tttttggatc ttcggtatca cgaatgcggt caatctgatt gacggtttgg atgggctggc
     5881 ggcagggatt tcattgatcg gtttaattac gataggaatc attggatatt ttttcctcca
     5941 tgcttctaca gtgtatattc cagttgttat tttctgtctt gtagcaagta ttatcggttt
     6001 ttttccttac aatttttatc cggcaaagat ttatttaggc gatacaggag ctctattcct
     6061 agggtttatg atggccgtcc tttcactaca agggctgaag aacgtgactt ttgtttcatc
     6121 gatttctttg ctgatcgtca tgggagtgcc ggtaacagac acgttttttg ctatcattag
     6181 gagaaaagca aaccgtgttt ctttttctac agcagataaa aaacatcttc atcaccgact
     6241 aatcagttta ggctttactc ataaaggtgc tgtcctgacg atttatgcga tggctttgat
     6301 gttttctttc acagctatgg tcatgaatta tactggaaga atcggaacga tcatattgat
     6361 tattgccatg ttgtttgctg caattttatt atttgaattg attggtctga tcaatgagaa
     6421 acatcagttt attttaaaaa cattgcgatt tttagggaat aaagagtacc gtacgcaagt
     6481 aaaacagaaa tatttgaaga aatggaaccg tcatatagat gaataacaaa ttaaaccagc
     6541 cgatccttat tagtattgtg acggcaaaca gtgataaaat ttttcggaca ctagataatt
     6601 ttatttcaac tgtaaaagat gaaacagcag taaaaatccg aatttttgac aacaattcac
     6661 ttcccgagta caaaaaaaga ctaacagagt ataggaaata tccctacatc gatatctatt
     6721 ttcatgatga aaattccgga tttggttatg gacataatca caatctgata gctagcgatt
     6781 ctcgctatgg cattatttgt aacccagata ttttagtgga tgaagagaca gttacagcat
     6841 tggtcttgtt attgaaagaa catccggaat gtgcgatgtt agcacctaag attcttaatg
     6901 aagatggaac aactcagcac ctcatccgtg agcagctggc tgtttttgat tacgttttgc
     6961 gctttgtgcc tttcaaatgg gtcaaaaaaa agtttgacaa acgactggct gtgtttgaat
     7021 gccgaggatt gcctactgat gctaatagtt atgtacggat gatctcaggt agttttatgg
     7081 tagctgatat tgataaattt aaagagataa aggggtttga tgagcgtttc ttcatgtact
     7141 ttgaagacaa cgacttgtgt ttgagttttg aaaaagcagg ttacaagtta ttgtacacac
     7201 ccttttatgc agttatccat ttttacggga agggtgcgca tcgaaatgca aaactgtttc
     7261 gcgtgttttt gacttctatg atgaaatttt tcaataagtg ggggtggcgt tttttctgat
     7321 gaaaccagaa atattagtag tcgttgtgct ttatcagcaa ctattcagtc aatctccagc
     7381 ttatggacca ttaagcaaag cacttagcga aaagcaaatt cagcttatta tttacgataa
     7441 tagtcctcaa aggcagcaaa atgagctgtt tgaaaaagaa aatatcgttt atcatcatga
     7501 tccgcaaaat cctggtttag cggtggctta taattttgcg ttgtctcaag cagatgaagg
     7561 aacacgttgt ctcgtcactc tggatcagga tacgaggcta gtcgacgatt attttgaact
     7621 attgcgtaaa gtaaccttta cagatgaatg tgttgctgca gttccgatga ttttttctgg
     7681 gagccgacaa gtttctcccc tatattcaga tcgttatatc aatcgtacag ctcaagcagt
     7741 agaagttgaa ggcccaacag ctgaacgaat catggcaatc aattcaggtg ttgcctggtc
     7801 tgtcaaattt ctaaaaaaga ttggcgggtt taatcctgct ttttctttag attttttgga
     7861 tcattggctg ttttggaaag tggatcagtt ggaaaaaact gtggagatcc ttcctgtacg
     7921 aatggaacat gatctttcgg ttttagacta tgaaaaagtt tcaagtaatc gataccgttc
     7981 tattttacaa gcagaaaatc gattttatca tgaatatgat caaaggcatt tatccagtca
     8041 ccgacgacaa ttattgttaa gaacagctaa acagttcttg accgtcaaaa accggcatat
     8101 ctggcggatg actttgcgct catatgttaa gaattggaag gtgtgaattc gtgcgttcgg
     8161 tttgtatagc tacctttaat ggaggcagat atttaagaga gcagttagat agtattttat
     8221 ctcagctttc agcagaagat gaagtagtca tttctgatga tgggtcagta gatgatacat
     8281 gggatatatt aactgaatac gcagaaaaag ataagcgaat ccagcttttt tcggggcctc
     8341 gtcaaggatt gatcgctaat tttggttatg cgatccagca gacgaaagga gatctaatct
     8401 ttttggcaga ccaagatgat gtttggttgg agacaaaagt gaaaaagatc actgactatt
     8461 ttgagcagaa tccggatcag ctagtagtag tttctgattt aatcattgtg gacagcgagc
     8521 tgcaagagat ccatgattcg tatttttcgt ttcgaaattc caaagaagga tggcttaata
     8581 atgtgataag aagtcactat attggtgctg gtatggcttt ccggtcttct ttaaaacccg
     8641 atattttgcc gatcccgtca gaagtgccga tgcatgatat gtggattggt ttgctgggag
     8701 gaaagaagac tgggttccta agagaaccgt taactctgta ccgaagacat gatttaaacg
     8761 cgagtgagat tgaaacacat tctaaattac ggcaaaaaat ttcttggcgt gttcatctga
     8821 ttacggcgtt gatcaaacga aaatggttta aacgttgata aaagatgttt aaatccttaa
     8881 tatttccatg acaaatataa caattgatga gataataaca aagtgaaatt tgaaatcaaa
     8941 aaggagagca gatatccatg aagggaatca ttcttgctgg gggcagcgga acacgtctgt
     9001 accctttaac aaaggcaaca tcgaaacaat tgatgccgat ttatgacaaa ccaatgattt
     9061 attatccaat gtctactctg atgttggcgg gaatcaaaga aattctgatt atttcaacgc
     9121 cacaagatac accacgtttc aaggaattat ttggaaacgg aaatgattta ggactgcaga
     9181 tcgaatatgc agtacaagaa agtccagatg gattggcaca agcgtttatc attggagaag
     9241 aatttattgg agatgatagt gtctgcttgg tattagggga caatatttac tatggtggcg
     9301 gtctgtctaa aatgttgcaa cgagcagctt caaaagatac aggcgcgaca gtatttggct
     9361 atcatgtcaa tgatccagaa cgctttggtg tagtagaatt tgatgagaat atgcaagctt
     9421 tgtcgattga agaaaagcct gctcagccaa aatcaaatta cgctgtaact ggcctttatt
     9481 tttatgacaa tgaggtaatt tctatcgcta aaggtatcaa accttcagaa cgtggagaac
     9541 tagagattac ggatgtcaat aaagcatatt tggataaagg aaaactatcc gtagaactta
     9601 tgggacgcgg atttgcttgg cttgatacag gaacacatga atcattgctt gaagcatcta
     9661 cctttatcga aacaattgaa aaacgtcaga acttaaaagt ggcttgtttg gaagaaattg
     9721 cttaccgtat gggctacatc gataaggaac aattgctggc ccttgcacag ccattgaaaa
     9781 aaaatcaata cggtcaatat ttattgaatt tggccgctga ataaggagtc cttacagatg
     9841 aaagtaacag atacaaaatt aacagatgtc aaaatcgtag aaatggacgt ctttggcgat
     9901 catcgcggtt tcttcacaga aagttattca aaagcaaaat ttgcagaaca tggattgaaa
     9961 tatgactttg ttcaagacaa ccattcacta tcagctgaag ctggtgtcct tcgggggctt
    10021 catttccaaa aaggagaagc tgcacagaca aaattgattc gggtagtcac tggagcagtt
    10081 ttagatgtag tagtggatat ccgcaaaggt agcccgactt atggtcaatg ggacggattt
    10141 atcttatctg aacataatca tcgacagttg cttgtaccta aaggatatgc ccatggattc
    10201 gttactttga caccaaatgt gaacttcctt tataagtgtg ataattacta taatgcagaa
    10261 gcagatgggg gcatcgcatt tgatgatcca gatttagcga ttgattggcc aattgatcca
    10321 tcaagagcga ttatgtcgga aaaagatcac cgtcatccga cattaaaaga atttgaagca
    10381 gaaaatccat ttgtttacgg agaaatctaa gaattaagga gaaattttct tatgaaaaac
    10441 atcattgtta ccggaggtgc cggattcatc ggctctaatt tcgttcatta cgtggtaaac
    10501 aatcatccag aagtgcatgt cactgttttg gataaactga cttatgcagg aaacaaagag
    10561 aacttggccg gacttcctag cgatcgcgta gaattggtcg taggagatgt tgcagatgca
    10621 gaattagtgg accgtctagt aaaagaagcg gacgcagttg ttcattacgc ggcagaatcc
    10681 cataacgata attcattaaa agatcctttc ccatttgtac aaacgaatat cattggtacg
    10741 tatacacttt tagaagcttg ccgcaagtat gatgtacgct atcatcacgt atctactgac
    10801 gaggtttacg gcgatcttcc attacgtgaa gacctgccag gacatgggga aggcgaagga
    10861 gaaaaattca ctgcggaaac accatataat ccttcaagtc catattcttc tacaaaagct
    10921 ggttcagatc tgttagtaaa agcatgggtg cgttcttttg gcttacgtgc aaccatttca
    10981 aactgttcaa acaactatgg tccttatcag catattgaaa aatttattcc tcgccaagta
    11041 acgagtatct taagcggtat tcgtccaaaa ttatatggcg aaggaaagaa tgttcgtgac
    11101 tggatccata cgaatgatca ttcttcagca gtgtggctga ttttgacgaa aggcagaatt
    11161 ggcgaaacct atctgatcgg tgcagacggt gaagaagata ataagaccgt gatggaattg
    11221 atcttagaaa tgatgggcca accaaaagac gcatacgacc atgtaaacga ccgcgcaggg
    11281 cacgatttgc gttatgcaat cgattcgaca aaattgcgag aagagctagg ttggcagcct
    11341 gagtttacga acttccgtga tgggttggct gacacgatca aatggtacga agaacatgaa
    11401 gactggtgga aaaaagaaaa agaggccgtt gaagcagcat atgccaaaaa tggacaataa
    11461 tacagaggaa atttcactgt attaatggaa aaacacatgt gaggcaggcg ctcgcctgtc
    11521 tcttttttaa aataaaaatc aaggagcgga taaccatatg tttttattaa ctggaggaaa
    11581 tggacaatta ggtacagaat tgcgccattt attagatgaa aaaaatctgc catatgtttc
    11641 aacagatgca aaagacttgg atatcacaga tgctgagaaa acaatggctt atattacaga
    11701 attaaaaccc gatgttattt tccattgcgc agcttacaca gcagtagata aagcagaaga
    11761 agaagcaaaa gaattagatg agaaaatcaa tgtcgatggc acaagaaatg tagcacttgc
    11821 agcaaaagct gtgaatgcac aattcgtcta tatcagtaca gactatgttt tcgatggaaa
    11881 aaacaaaaat gtagaatacg aagtagacga ccaaacgaat ccactaaatg aatatggtcg
    11941 tacgaaatta ttaggcgaaa aagcagtaga agagatttta gaagactatt atatcatacg
    12001 aacttcttgg gttttcggga tctatggaca caatttcgtc ttcacgatgc aacgcctagc
    12061 ggaaacccgc gacaaactga ctgtagtaaa tgaccagttt gggcgaccaa cttggacacg
    12121 tactttggca gaatttatgg tatatgtcat cgaagaaaaa gcaccatttg gtatttatca
    12181 tttgtcgaac gaaaatagct gttcttggta tgagttcgca aaagaaatat tgaaagatac
    12241 agatgtagaa gttgcacctg ttacgtcaga agaatttccg caaaaagcaa cacgtccgca
    12301 gtattcagtc atgagcctga aaaagacaga agcgttaggt tttgtcatac caacttggca
    12361 agaagcactt gcgcaaatgc tggaagctgc caaaaaaatg aataagtaat ttccttttta
    12421 tcaaatagaa tttggagcat gatatggaaa aacaaacaag aagtgcacga cgaaaagaaa
    12481 agccaaaagc gaatcaggca gactcgaatt tggtgcttga cctgtttcta aaactccgct
    12541 atcttattgg cgttttagtc atcgtggtca tcgttacttt taatttaaac ggaagttcac
    12601 ttggtgtttg ggataaatat gtgtcgcaaa gagacgatgg gaaaaaaact gatgtgattt
    12661 ttggacagaa tcgggaagtg cggtcagacg aatggctggt gcagacacct ttttacctct
    12721 cacaagctga agaggacttt tcgttggtga acgaggatta ttcattaaac ggacaaaaca
    12781 tgatcattgc gtataattct cctgtaaagg atattacggt tatcggaaaa ccattcaact
    12841 ggggattctt tttcctcgga agagatcgag gactttcctt ttattgggcg atgaaattag
    12901 tcgtcatggt cttgcttgga ttcgaagtac tgatgatatt aacgaagagg aaaaaagctc
    12961 tatcgctaat cggtgcattt gcgctgacat tttctccagc agtccagtgg tggttcatgc
    13021 aacatgtggg cgatttgatt ttctttactt tgggactgat gattgctttt tatcattatt
    13081 tctacaatca tgataaaaaa tggctacgaa cgttgatgat gcttttggtg gttattttcg
    13141 gtattgggtt tattttagtg atttacccag cccatcaagt gatgctggcg tacttgctga
    13201 ttttttattt tgttggcctg cttatttact atagcaagaa agtttcatgg gatctgttgg
    13261 atgtagtgtt gattggaggg gcagttttat ttattggcgg agtcatggtt catttctggc
    13321 taacgtcaaa agacgcattg ttagcttctt tgaatacctt atatccagga aaacgggtaa
    13381 gtactggagg aaagtggacg attggcgaat ttttttcttt ccttacaaat tggaaaattc
    13441 cttttgaaga tatcaactat tcgaataact ctgaagtggc cctgttctat cacttttttc
    13501 caactgtatt cctagcaagt ccatttgtgt tatttggcaa aaaagatagc gaacaaaagt
    13561 tactgggacg tattttgatg attttttgtc tgttttctat cttttggatt accgttgggt
    13621 tgccaaaatg gctagcagaa atcactctgc tgtcttatgt tcctccatta agagccatcc
    13681 ttacttttag ttttgcggct tgtttattat cgatctggtt tatcgcttat atttggagag
    13741 aagatgttat tcctatttgg tataaattca ttttgttggc agtaaatgca tggtcttatt
    13801 tctatgcgat tacgcattcg aaaatggata attatttttc ggattttgaa accatcgcag
    13861 ttgctgtgtt gggaacagtg attttagctt ttgcattatt tggctggaga cgcttgtttt
    13921 acctcgcttt tgtcgcctta gttatcgtat ctggcttttt cgtcaatcca gtcgtagaag
    13981 gtacaggagc gatttttgaa aaaacactgg caaaagaaat ccaaaaaatc gatcaacaag
    14041 atcctggtaa agtttggttg tctgaagatg aattatataa cttcacccca acattgggag
    14101 tgaaatcctt caatacagta cgcttttatc cagatatagc tgcatggaag aaaatcgatc
    14161 cgaaagaaga atacgagaag tattataatc ggtatgctca tacaagagct tttatcgcta
    14221 aggaagatac aaagttcatt ttagatcgtc cagatatgtt ttcggtcacg ttgaattttg
    14281 aagacgcaga aaaattaggg atcgactatg tgatcagtaa acaaccatta actgattaca
    14341 accaattata tgaagcgcag ttcacgcagc tatatggacc ggataaagac ggttatatga
    14401 tttttaaagt agaatatccg gattatccag atgtaccaca aacacaaact caggaaaatc
    14461 agctagtcgt acagtaatag gctagctgag aataatcctg aaatattagt agaaaggaga
    14521 gtgaagataa ttcaagcaat cgtacaaaaa tttgtcaaca aaatgagtgt cggtttcttg
    14581 cttttgttgc tgacgctagt cttttttgtt tggacagcag gggtaggttt tccagcaact
    14641 ttgtttatat ataacaatag ttcattactt atcgctttta gtgtggctat tttgctcatt
    14701 ttaaatgttt ataaagcaaa aggcgtcgat tttctttatc taggtgttgc gcttgccctt
    14761 ttcattttct tttcttactt tcgggaatta agaaatgagt ctttatttgg agacttactg
    14821 tttcctttga ttattttatt ttgtttagta attaaatgga tcgagtggac aaaatgggat
    14881 cgcggtattt atctgattgt ctttacggtc ttcctcggaa tcacgttata tcgtgtattt
    14941 acagagatcc aagtaccaga aggacaaagc atctgggctc caggcaattc gctttctgat
    15001 atttggatca atacgaatac aatcggaagt tctttgatga ctcttgcttt attgatcagt
    15061 ggatttgcca gttcttttga acgatggtat gttcgaatca tcggtgggat agcagtcgtt
    15121 gcagcctttt taggtatttg ggtctgccaa tctagaggtg cattagtcgc gttgattgtt
    15181 tttgcgatat tggatctttt acctaaatcg ttgatgcgtg tcattcgtgc gccatttatt
    15241 gcctacacgg ttacgattct tttagctttg ccgatttctt atctggcggc tgtctctgaa
    15301 aaagtcaatt tgtttaccgg tcgagaagat atttggcata aattctatca gacgctagga
    15361 gaaaaatctg aacaagtttt actaggaatg aaaacattta tcttccagcg aggaaaccaa
    15421 tttttaggaa accacaatag ttataattct attttgaata tctatggatt gataggattt
    15481 gggattgctg ctttgctgct tatccttttt attggtcgct taacgttgaa agcagattta
    15541 tccaacgggc agatgacttt tatctgggcc ttttttgcgg tgatgatgca gtcatttatg
    15601 gaggatacat tgacttcata cccatgggtt ccgattattt atatcctgtt ggctatggcc
    15661 gttcatcgat atgatgaacc agaagttacg gaggaattct atgttatgga agaggcatat
    15721 gacgatatga cttatgatga tacatcttat gaggaacaac cagatagcgc tcattcttcc
    15781 aggatccaac gctatcattg ataggcacaa aatacgagag aggctgtgac aaccgtcgca
    15841 gccttttttg gtttattgca attgtctagc atcttctgta aaatagaaac gttagagaaa
    15901 atcttgtatc atagaaaatt aagaaagaaa taagaacgca gctcggtacc aaaaatatgg
    15961 taaaatggca cttgactgta attcactgaa aatcgcttgg aggaacacaa atgtttaaag
    16021 atttgtattt atttatgaag gacatttatc agaacagaaa attactgcta cagttttcga
    16081 tcaatgactt caaatcaaga tatgcaggat cgtttttagg aatcttatgg gcgtttatca
    16141 atccgctatt tactgtattg atttattggc tggtatttgg tgttggactg aaagcacaaa
    16201 tgatcgatgc aaaataccca tttatcgtat atttagttac cgggatgatt ccctggttct
    16261 tcttctcaga tgcatttgca tcaactacgg ttgtttttcg ggaatatact tatttagtaa
    16321 agaaagtcgt attcaatatt cgtatcttgc ccacgacgaa aatcctatcc aatctgtaca
    16381 cccatttatt cttcattctg attggcatag ctgtcgcgct tataaatggt atatatcctt
    16441 cagcaatgtc tttacagctg atttattatt tattttgcat gtgtgctttc ttgacaggat
    16501 tgacttggct gacggcatcg atccaaccat ttttacctga tatcatgcaa ttaattacga
    16561 tcgtgctgca gttgatcatg tggacattac cgatattatg gagccctaat cagtttgatt
    16621 caggtcttgt aagcatattg aaattaaatc cgctgtacta tgttgttcaa ggttatcgtg
    16681 agtcattttt aagtgaagct tggttctggg agcatggaac atatactttg tatttctggg
    16741 ttttcacgtt agtaatgttc gtaatcggct ctgcagtgtt ccgtagattg aaaccacatt
    16801 tttcagatgt gctgtaagga gaatagctat gacagaatat gcaattagac taaaagatat
    16861 cacaaaatct tataatatgt acgcaaaacc ttcggatcga tttcgtgaag ccctcaatcc
    16921 ttttaagaaa tcctatcatg atgtatttta tgcgttgaaa aacgtgaatg tagaaataaa
    16981 aaaaggtgag atgatcggtt tcgtgggaga aaacggatcg ggaaaatcta caatgttgaa
    17041 aatcattacg ggagtactga caccaacatc cggcacgatg gaaatcgaag gaaaaatctc
    17101 agctttgcta gaactaggat caggattcaa tcctgagtat tctggctatg aaaatatcta
    17161 tttaaatgga atggttctag gattttcaag agaagaagtc gatgaaatgg tcgatgatat
    17221 cattgaattt gctgacatcg gcgaccatat ttatcaacct gtaaaaacct attcaagtgg
    17281 gatgttcgtt cgtctagctt ttgctgtagc gatcaatgtg aatcctgata ttttgattgt
    17341 ggatgaagca ttggcagtag gagatcttga atttcaacta aaatgtatgg aaaaatttac
    17401 agaaatcaag aactcgggaa agacgatttt attcgtttct catgatatta gttcgattcg
    17461 ccgtttttgt gatcgttcct attggttgaa aaatggaaaa gttgtcgaat atggtgatac
    17521 gatggaagta acgacgaatt acgaaaactt tttgaagaaa aaatcagtta aaacagtaga
    17581 tcgaaataaa aaccaagtag aacgcttcgc agctccagat atcgtagacg tgacgaaagc
    17641 cgaattgttg gataaagata aaaatccagt ggatatgctg gaacaaggag aaaaactata
    17701 cgttaaagta acctatgaag taaaagacga tacaatcaaa aatccagttt taggaattgc
    17761 tctgcgtaca gtcgacaatt tttatgtgtg cggactcaat actctactag atggtatcaa
    17821 gatcccttgg aaaaaaggtg agaatatctt ttatctggag tatgaaaagt taggtttgtt
    17881 agctggtgaa tattattttg acgttgcggt ctttgaagaa aatgcaacgg ttcctttggt
    17941 atacaagaca aaatacatga acttgtttgt cagcggttct tatatcggag aaggcatcgt
    18001 ggtcttagac cataaatggg aagaagggac tcatagcaat gaaatatgat tttgaaatgg
    18061 acctagatga acaaagcagt gtgggaaaaa ttgccgcaca gatcaaacca ggaagtaaag
    18121 ttttagagtt tggtcctgga aacggccgat taacgaaaca tttgatcgga gcaaaacaat
    18181 gtgaagtgag cattgtcgag ctagataagg aattgttcga ttttgtcagc gagtttgccc
    18241 aagatggatt ttatggagat atcgaaagtt ttgaatgggc aaactattat gctggacaaa
    18301 catttgatta cgttttgttt gccgatgttt tagaacattt ggtagatcct ggaaaaacgt
    18361 tgaagaaagt tagagaattt ttgaatgaag aaggagaaat tctaattaca ttccctaacc
    18421 ttgcgcataa ttctgtcatg attgatttgt tcaataatca attgccatgg gcttcttatg
    18481 gtttgctaga tgaaacccat aattcttttt atactcatga tggcttccaa aaagtttttg
    18541 aaaaagcagg attatacatc aatattgaag attatttgta cttagctgta ggtgatacag
    18601 aattaaaatc aacttatgaa gaattgccgg aagctgtacg ttatgatttc aaaatgcgac
    18661 catttggcga agtctaccaa tacttcttct cattgatgaa acatccagta gcacaaagtt
    18721 ccattgcgca gccgcaaaat tctaattatg tgaaagtatt agaagtaatc cagcaaacga
    18781 aacaagatga aataattcaa caaataccat tcaataattt cacaggggaa aatgagacac
    18841 taagtttcca tgtatcagaa ttagcagaac gaatggtgtt tcgcttcgcg cagcaaccta
    18901 gctttatcga attcagcgca gaagctgctg gagaaaaact cacatttatc gattcaaatg
    18961 ctgtagtcaa aacagtaaat gattgttatt tgtttgatgg taaagattta ccagagttct
    19021 ttttaacgga tattgcaggg aaagaagtga ctatccattg tcactatcgt tttatcggag
    19081 aattgacacc aacaatgaaa gaattgctgg aaacaatccg tccaatggct gaagttacaa
    19141 agcagctttc tgagaaaaat gatgaattag aaagagaaaa tcattatctt attgagaata
    19201 atacaagatt agatcagacg ttgaaaacaa ccataaatcg ctattgcacg ttgttggaaa
    19261 gtgatgaatg gacaatcaaa catagattag ggcgacgtaa gaaagaaaca tcgaaaaaaa
    19321 tccaagaaaa agagctttct ctatgtattg acgaaaaaat ctgggatgca gaaacaaaga
    19381 tattgaaaat catcgggtgg ggaatcgcta attcggatcg ccaaccattg tcctataagt
    19441 tatcagccga ccaatcacca ttttttgaag caattcctgt tttacgagaa gaagtcaatc
    19501 aagcaaaaca gctagctgaa ggagcaaaag caggatttga actgcggatc ctttgcgaga
    19561 aggaacgttc attcttagtt gaagcagtgg ctcaaaatgg tcaatcatgg atcattgaaa
    19621 tgtaaataat gattaactta taaagataag gagtaaacga atcgtgcaaa aaaaagaaat
    19681 tgccgtattt atcgatcaaa tcactcgtga acgggccact ggcgatttgc ttattgtggg
    19741 ctgggcaatt gatgaagtga ccaaagaaat ccctacgata aaggtagaaa aagaaaatgt
    19801 gattgctgaa gcaacacatg tcgtaagatt ggatatcaat catttatata atcttgatgt
    19861 taagacccaa agcggtttta aggtacgttt atcagggaaa atgagaggaa aagcaatctt
    19921 agacttccaa acagcaagac atcaaaatgg catagctgtg aaattaaacg gtaaatatcc
    19981 ttatgatgac ggcattgaat cttcatggga aagaaaaaaa agattactga aaaaagggat
    20041 aaattatgca aggactcatg gcgtaaaaaa agcaatccgc cgggtcaaac tggaattgaa
    20101 accaggatcg atcgactatg cagagtggat cagtcgtcat gagaaacctg atctgaaaga
    20161 gcaaaggaaa gaagtacaag gatttgccta ccgcccattg atttccatac ttatgcctgt
    20221 ctataacgtt gaaatcaaat ggctcgaaaa atgcattgat tcggtacttg atcaaacgta
    20281 tgatcactgg gaactatgta tcagtgatga tgcttctact gatccagcta tccgaaagtg
    20341 tctagaatcc tatcaagcaa aagatgatag aattaaagtc gttttccgac aagaaaatgg
    20401 acacatcagt ctggcaacta attctgcatt gaaaatggca gaaggagagt ttatagcgct
    20461 cttagataat gatgacgaat tacctccatt tgccttatat gaagtggcaa aagtgctaaa
    20521 cgtacatcca gaactggatt tgatctacag tgacgaagat aaaatcgatg cagatgggaa
    20581 tcgctttgac ccgcacttca aagcagactg gtctcccgat acattgatgg gaaataatta
    20641 tatctctcac ttaggcgtat atcggacaag cattgttaag gaacttggcg gtttccgtaa
    20701 aggatatgaa ggctctcaag attatgattt ggttcttcgt gtgacagaac aaattcctgc
    20761 agaacacatt taccacattg accgtgtctt gtatcattgg cggacgatcc cgggatcaac
    20821 tgcaagcaat ggagaagcta aaagctacat ctatgattct ggagtgaaag cattgacaga
    20881 tgctctatct agaagaaata tcaagggaag cgtacatcct ggcagaattt ctggttttta
    20941 tgaaatcact tatgatgttt tgcaagaaga cttggtcagt gtgatcattc caactaagaa
    21001 tggctatgaa gatctaaaaa cttgtgtgga ttcgatcatc gaaaaaacgt cttatccaaa
    21061 ttatgaaatc attattgcag ataatggaag tacagatcct aaaatgcaag aattatttgc
    21121 agaatacaag catcagctga aagaccgatt tatcgtcgaa ttaattgaca ttccattcaa
    21181 ttattcacgt atcaacaatc tggcagcaga aaaagcaaac ggcaaatact ttttattctt
    21241 gaataacgat acagaagtga ttgaacctga ctggatgaca gcgatggttt cttatgcaca
    21301 gtttgatcga atcggctgtg taggagcaaa attattttat ccggatgata caacgcagca
    21361 tgcaggtgta ttattaggga tcggtggcgt tgcaggacat gcattgaata actatgatcg
    21421 aacacattgt ggttactttg gccgattggt cattgacgtc aattacttag cagtcactgc
    21481 agcttgtatg atggtcaagg cagctgactt caatgctgtc aatggatttg atgaaatatt
    21541 agaagtagca ttcaacgatg ttgacttgtg cttgaaagtt tatgaactgg gtcgttacaa
    21601 tgtctatgca catcaagcag aattgtacca tttcgaatct aaatcaagag gttatgaaga
    21661 tacaccagaa aaacaaagac gttttgctgg ggaaatcaaa aaaatgcaag ataagtggcc
    21721 ttcttatatt gctcatgacc cattttataa cgataatcta actaaagaag gaatcggcga
    21781 tttttcgttg agacctgatt gattaaaaga actgtatttt catttttatt aatga
//
LOCUS       DAXWOO010000034        21746 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.034, whole genome shotgun sequence.
ACCESSION   DAXWOO010000034 DAXWOO010000000
VERSION     DAXWOO010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.034"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(211..1110)
                     /locus_tag="ACGE70_001002"
     CDS             complement(211..1110)
                     /locus_tag="ACGE70_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFF8692652.1"
                     /translation="MGKLAIISDLHVDIKKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYEPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            complement(1120..1476)
                     /locus_tag="ACGE70_001003"
     CDS             complement(1120..1476)
                     /locus_tag="ACGE70_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFF8692653.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            1832..2128
                     /locus_tag="ACGE70_001004"
     CDS             1832..2128
                     /locus_tag="ACGE70_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFF8692654.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            complement(2772..3725)
                     /locus_tag="ACGE70_001005"
     CDS             complement(2772..3725)
                     /locus_tag="ACGE70_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFF8692655.1"
                     /translation="MKKLVVGILILASFGLAACGNSDNTSQADTKKSSTQAETALTIK
                     DSNGDQIEVPKNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(3748..4503)
                     /locus_tag="ACGE70_001006"
     CDS             complement(3748..4503)
                     /locus_tag="ACGE70_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8692656.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKKLVQEIGKTIVIVLHDINFAASYADEIIAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(4500..5459)
                     /locus_tag="ACGE70_001007"
     CDS             complement(4500..5459)
                     /locus_tag="ACGE70_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFF8692657.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFAWALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(5452..6402)
                     /locus_tag="ACGE70_001008"
     CDS             complement(5452..6402)
                     /locus_tag="ACGE70_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFF8692658.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFPQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            6552..7004
                     /locus_tag="ACGE70_001009"
     CDS             6552..7004
                     /locus_tag="ACGE70_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFF8692659.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPVEKEKPKKKKSFWDRLF"
     gene            complement(7092..9359)
                     /gene="gshAB"
                     /locus_tag="ACGE70_001010"
     CDS             complement(7092..9359)
                     /gene="gshAB"
                     /locus_tag="ACGE70_001010"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFF8692660.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEAIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWKEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDGIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(9546..10544)
                     /gene="ftsY"
                     /locus_tag="ACGE70_001011"
     CDS             complement(9546..10544)
                     /gene="ftsY"
                     /locus_tag="ACGE70_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFF8692661.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKIEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(10556..11365)
                     /locus_tag="ACGE70_001012"
     CDS             complement(10556..11365)
                     /locus_tag="ACGE70_001012"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFF8692662.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMVHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(11374..14955)
                     /gene="smc"
                     /locus_tag="ACGE70_001013"
     CDS             complement(11374..14955)
                     /gene="smc"
                     /locus_tag="ACGE70_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFF8692663.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLLKEVAEKETEIQKAEASLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYVQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(14975..15661)
                     /gene="rnc"
                     /locus_tag="ACGE70_001014"
     CDS             complement(14975..15661)
                     /gene="rnc"
                     /locus_tag="ACGE70_001014"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFF8692664.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(16009..17790)
                     /locus_tag="ACGE70_001015"
     CDS             complement(16009..17790)
                     /locus_tag="ACGE70_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFF8692665.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(17816..18730)
                     /locus_tag="ACGE70_001016"
     CDS             complement(17816..18730)
                     /locus_tag="ACGE70_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8692666.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(18757..19719)
                     /gene="opp4B"
                     /locus_tag="ACGE70_001017"
     CDS             complement(18757..19719)
                     /gene="opp4B"
                     /locus_tag="ACGE70_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFF8692667.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(19719..20663)
                     /locus_tag="ACGE70_001018"
     CDS             complement(19719..20663)
                     /locus_tag="ACGE70_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8692668.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(20664..21668)
                     /locus_tag="ACGE70_001019"
     CDS             complement(20664..21668)
                     /locus_tag="ACGE70_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8692669.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
BASE COUNT         6074 a         4659 c         3851 g         7162 t
ORIGIN      
        1 gtatattata tttttttttg tgattatcgt tcaaaaaaag aataaacgct tgcatctagt
       61 actctgcgag ttacaaatca tccgatcaaa taaacattcg ctgattattt tccacaaaaa
      121 aagcagcata tccacgataa atggatctac tgctcttact tatccttatg ttttacaagg
      181 tattttcttt tacacattac taatgctgtc tcaatacgga acgattgtca tcgcctgttc
      241 aaattcctct tgcaaatgtt gtgtttgata ggcttgaaag gccggatgat gggcacgcaa
      301 gaggacacgt aatttcatgg gttcatagct atctaacagc tgttctttca agacaaaatc
      361 tcgggtcagc tgccattcat aataataacc aaaaggtctg cagctgtaaa tcgtgtgacc
      421 gatggattta tcttcaaagc gacgatgcgt atggccaaaa actacttgtt tgatatttga
      481 ataacggtca agtaattctc cgaatgattc tgatcctaaa aatgcattta atttgttcca
      541 gcgctcataa ggagcagatt gatacatgat aaatgcccgt ttaggaacga aatgagtgga
      601 taaaatcacc tcaagttttt ttgcttccag ctggtctagc aaattttttg cagctgcaag
      661 catttctgca ttgacctcga gatctgtttt cccacgctgg atgaaccgat cataccaata
      721 caaacgcttc attcgtacga ttgcctcgta atctttcaat tcggaaaact gatagtcgta
      781 ccagccattc atccctaaaa gaacggtctg ctcatttaaa gcaatatacc gttcatttaa
      841 aaaatgtggg tctggaaaat ggtccatcat ctgttcattg ggcacatctg ccaactcgtg
      901 attgccgaaa tgaaatgtcg tagataatcc tttattgacg aaaaattcga caacagctag
      961 gcaacggtct actttattag ccgtatctcc ggctagatga agccgagtga tcttgtgctt
     1021 ttgcaaaact tgccaaagct gcatcagctc tgattcgccg aatttcttga tgtccacatg
     1081 taggtcgctg atgatcgcca attttcccat tttatccctc tagtcttttc gaacaacttg
     1141 caagttgcct tttctcaagc cgcttggtcc ttttaattgt aaactattat aagttggttc
     1201 taaagcaagt gtggatggat catccatgta aagtattgca ctgtctttga ttttgattgg
     1261 tccaactggt gtatcagcca ctacagaata aagacttaga tctgtttgtg tatctttttt
     1321 gacaatgatc aatcgaaatg ccgtgtccaa ttggcaactg acttggcttt ctgcaaatgg
     1381 accgtcaccg ttttcgatat ctaataaaag ttgatcgtct gcttctaatt tattcttcaa
     1441 tacttcttgt gcttcttttg tcacttctaa tttcatctgt atcacctcat gtcatagaat
     1501 aatcacttta ttcatcagat gcaactaatt tgtctggttc tgactgttct caatttattg
     1561 gaaagtaagt cattcgttta ttatgatttc tcttatcaca aaagagacaa ctttcccttt
     1621 tcttcgttca gtttcttgaa cggaaaaaga cccaaaaagc aacctgttat ttgtttaacc
     1681 ttattccttt cttaaataac aaaaaatact ataaaaaata aaaaaccttt aacaactatt
     1741 ggaaaaagaa ttactttttg ttcacggatt attaacaact cttcggtaga gtaaacttat
     1801 caaataaaaa gacttctttg gaggaatcac tatgaaggaa tatataaaaa acatctattt
     1861 tatcgaagaa acacaaaata tcgagggttc ttatattgaa gtaaaaacac tctttgtcaa
     1921 tgaagataaa accaaagcat tggatattta taaaaaatta gcttcaaaaa aaacgaatag
     1981 ttttggattg atattaagcg agtataaaat caaagcagaa gagagttatt tctatcaatt
     2041 attaaaacgg tggagtaagc ttcccgccga tttttatcga aaaatgcaga tcatcaacta
     2101 tcaaccgctt gctgagacac atgcctaaat agaggttgtg aaatcagtgc tttgagctaa
     2161 taccgaaggt cagctgatgg aacatcgttt agaagagatt gtgacaacta cgatttgctt
     2221 taagtaataa gaacgctgga cagaaaatag cttctcatat tttctagtct agtggactta
     2281 ttgccgagaa gcagcagatg gaacatcgtt tagaagagat tgtgacaact gcgatttgct
     2341 ttgagtaata agaacgctgg acagaaaata gcttctcata ttttctagtc tagtggactt
     2401 attgccgaga agcagcagat ggaacatcgt ttaaagaaga gatcggtaca aaataaaaat
     2461 atctaaattt aagttttatc taagtttcgc tttgtacctt tgattcaaat caaaggaggc
     2521 gatcttgatg ctgaaaaaat ggatcacact attttttgcc actccccatt ctagaatcaa
     2581 cccgtatgat gtagagaatg aattcacgtt gaaaaaagat ttcccaaata aataaaaacc
     2641 atccgtaaaa tcatcaatac gattttacgg atggtttttg ataacttctt tgaggatagg
     2701 ctcttgctat ttaagaatgt aagaaacatt attgttacat agtatgagag tttaatcctc
     2761 tgttatttat gctatttaaa tgcttgattc acatcttcga tcatcagttt cattgattct
     2821 aaaccgcccc cgcttaaata ccagacatct ggttcaaggg agatgatctg ctgattttta
     2881 ccagcattcg tttgtgctac cagttcattg gcagaaatat catctttgct gtcgtctcca
     2941 ccaatagctt ttgttctgtc tactacaaat aagatatccg gatttttttc cagcacatat
     3001 tcataagaaa cactttgacc gtgagtggat gcttcgattt gatcatctgc ttgttcaaag
     3061 ccaaataaat catggatgaa accgaatcta gaaccagatc cgtaggctga taattgccct
     3121 tcattcacaa gtgtgactag tgcttttttg tcagtagctt ctgctttctt tttcgtctga
     3181 tcgattgcac tagataattc ctctaattga gttttagctt cttcttgctt accaaagatc
     3241 tcccctaaag cagtgacatt ttgctggaaa gattcccatg gatttttcgc atctaaagaa
     3301 aggaaaatgg tcggagcaat ttgacttaga tctttttgaa aatctctttg ccggccagaa
     3361 atgatgatca ggtctggctg aagctgattg attttttcta agtctggttc tttgatccca
     3421 cctgctgatt ccactttttt ataggatgaa agatactcag gaaggctgct tgtagctgct
     3481 ccaactacct tatctccaac accaagagca tccatcgtat ctaaagaacc attgtcgaat
     3541 acgacgactt tttcaggatt ctttggcacc tcgatttgat cgccattact gtcttttatc
     3601 gttaatgccg tttcagcttg cgtactgctt ttctttgtat ctgcctgact ggtgttgtca
     3661 ctatttccac atgctgctaa accaaatgat gctagaatca agatacctac tactaatttt
     3721 ttcatctctt ctctcctaat gttagtttta gaaataaaga cagaatctgc gaccttcaat
     3781 ctcacaaatt cgtatgtcca tttcgtacaa ttcgtccagt acatcttttt ttatcatttc
     3841 ttgggtcgtt ccttgcgtga ataactgccc atctttcatt gcaataattt cgtctgcata
     3901 acttgcagca aagttgatat catgaagaac aataacaatt gttttcccga tttcttgtac
     3961 taattttttt aagatctgca tgatttgaac cgcaaaattc atatctagat tgttcaaagg
     4021 ttcatccagt aaaatataat ccgtgtcttg cgctagcacc attgcaatat acgcacgttg
     4081 gagttgaccg cctgaaagag tgtcaatcga ttgatccgct atttcttcta acccaaggtg
     4141 ttccagtgat tcctgaacga tttcctcgtc ttttgtcgtc aattttcctt tcgtataagg
     4201 aaatcgtcca aattctacta attctttcac ggtgatttgg acatttattt cattcgattg
     4261 tcgtaggatc gataattttt tggctaattc attggatttc caagccttta cttcgttatg
     4321 gtctaaatag atttcacctg tatcttttgg tatcaaacga ctgatcatcg acaacaccgt
     4381 tgactttcca gcaccatttg gaccgataaa agcagtcaac ttcttttcag gaatcgttaa
     4441 ctgtacatct ttaaccactg attttccatg gtacagttta ttgacctttt gtaaatcaat
     4501 cataggatac tcttcctttc gcgaatcaat tttgtgacaa aataaattcc tccgacaaat
     4561 tcaacgacga cactcactgt tgtattccag ccgaaaatct gctctactgc gagctgtcct
     4621 cctgtcagaa acagcatagc taccagaaag cttccgataa atagctgacc atgacgataa
     4681 gtagaaaaga cacgataact gatatttgcg acaataaacc ctaaaaaagt aactggaccc
     4741 acaagagccg ttgatgtccc tgtcaaaaga ctgacacaac ataaaatcaa aaattcaaaa
     4801 cgatctgtgt ctatcccgag gtttactgct tgttcatttc cgagatggaa aacgtctagc
     4861 acatgtcgat atcgaaataa caggtaccag cagccgataa tgatcaaaga agcaggcaaa
     4921 agatactgac tctggatatt gccaaagctt gcaaataatc tgccttgaag caaatcatat
     4981 tcattcggat ccatgatgac ttgtaaaaag gtactgacac taccaaaaag cgtacctaaa
     5041 atcatccctg acatcagtaa taaaaaaaga ttcgctctca tttttttcaa aagaaagaaa
     5101 aataacaata gactcagccc gaccatcagc ccaacgttca ataagaaaaa aactgtacct
     5161 gtctgctgaa gcatcgtcac tcctccgata aagaagaaaa ggaccgtttg gacaaggaca
     5221 tataacgact caagtcccaa tactgaagga gtcaaaaact ggttatgcgt aattgtctga
     5281 aagctgattg tggcactgct gacagctagc gagacaagga taaatgcaaa aattttctta
     5341 ctcctcaatg cccatgcaaa atgccagttt ccatatgtat ggatcgtcaa gtacattcct
     5401 gtaacaaata aaacaagaac aagcatacca ataaaaatag atggcttttt tttaagcata
     5461 tcgtcgcccc cttatcagaa gataaaggaa gatagcccca ccgatgattc caacgatcac
     5521 actgactggt atttcataag gaaaaatcaa cacgcgagcg acaagatcgc aaaatactaa
     5581 gaacaaacta cctgctaaag cagttacgcc aattgttttg tgcacttgat ctccatatat
     5641 tctagagaca agattcggaa tgatcactcc gataaaagga atgcttccta ccatgatcaa
     5701 catgactgca ctagccaatg atactaagcc aatgcctaaa aactgcatct tctggtaatc
     5761 tattcccaaa tttactgcaa acgattcgcc cagaccaatg atcgtaaatc ggtaagcaaa
     5821 tagccaaacc aagagcaaca atggaacaac agcatacaat aactcataat ttcctcgtat
     5881 cacagtcgca aaatttcctt gaagccatga agtcatattt tgtaccaact ggaattgata
     5941 agcaaaaaaa gtagcaactg aaccaatgat attccctaac atgactccaa tcaacggaag
     6001 catcaattga tttttctggg ggaacaatcg cgtaagggat aaaaaaagca atgttcctgc
     6061 aaaagcaaag caaaatgctg tcaaggatcg gacaatcaag gatgcaccag gtaggaaaat
     6121 catagccact aaaataccta aacgcgcact atccatcgtg cccgcagtcg ttggtgaaac
     6181 aaatttattt tgggtcaaat gttgcatgat caacccacaa atactactgg tcgctcccac
     6241 aaggatcaaa caaatcgttc gtggcaaacg agtcactagc aagatttgta agctttgccc
     6301 atctaactgg agcagttctg acaattgtac attcgatact ccgatgaaca atgaaatact
     6361 acttaaaata actaataatg ctactagtcg tccgtagcgc atccaatcca cctttctaat
     6421 tgagaatgat tttcattctt attttaagtg aatcgttgaa caaaagcaac cgctttttca
     6481 tatgttttag gtattattgg gaatatgcta taatcgtaag aaagacaaag agaagaagga
     6541 gggtattttt tatggcacaa gatgatgtaa aaagccattt ggataaggga ctttatggta
     6601 cacctttagt gaaccctgaa gaacagcata aatatttagg gacattcagg gaacgctgct
     6661 atcttagtat gactgttgct gaaatgatcc aaccaaaaaa caaagaaaac tttttaaaag
     6721 aactcaaaaa acatccagat gcttccatac tcttgaatgg ggctctaccc ctttctgtcc
     6781 aaaaccaata tatccagcta gcaacgaaaa acaatagccg atttactgta gtcaacgatt
     6841 acgtcactgc aaatcctgag gaattcggat tgctcttaac agcaaaagaa gcagtaaatg
     6901 aacctgtgat tgatatcgaa aaaaaatacc cagaagaaaa ctcttcgcca gttgaaaaag
     6961 aaaaaccaaa aaagaaaaaa agcttctggg atcgtttatt ttaaaacgaa aaaacacata
     7021 aatttttaca tgcaaaaaag cttttgatca gattgacatc aaataggaca atctgtcaaa
     7081 agctttttct tttaaaatac ttcaggatat aacaatttca atacattcat cgtcaatctt
     7141 ctgccttttc ctgcaaaggg ataaacatgc atatgcatgg ctggattgaa atttgcttcg
     7201 atgatcccat atgctttttt atcagttgta ggatcgattt ctttatctgg tataatcaaa
     7261 tcgataccac taatcttagc tcctagtgct tctaccgcag aaacggcgat ttttttgtaa
     7321 gcttcagaaa attcatcagt catatcgatc gaatcaccac ctgtactgat atttgagttc
     7381 tcccttagat aaacaatttg attggcttgg ggaactgatt cgatcgtcaa cccttgttct
     7441 ttcaacatca attgttctaa ttcacctaat tggatcaatt ctaaaggtgc acgatgattc
     7501 gttccacgca aagggtcact atttttttct tcaacaagtt ctttcactgt tcggataccg
     7561 tcaccaataa cattagcagg gactcgcaac atgatggctt ggacttctcc gtcgatcaca
     7621 aagaaacgat attccgttcc cggcataaat tcttctacta aaaccgaact gtcttcacga
     7681 aaagcaatgg ctaaacctgc ttgataatct tccagactag ctccttcctt gaaaatcgtg
     7741 atacctaacc cgtaattggt tgatttaggt ttgatcacaa atgcctgctc ggcaaatctc
     7801 ggataagctt ttaccgcttc ttccatggat gaaaattctg cgcctcctgg tacccggaac
     7861 ccagcttctt ttaagacttt tttcgttact gttttgtttt ccatgataag tgggacgata
     7921 tagctgtcct tacttgtcat gtttgcattt ttcacatatt ctacatggtc ttggaattgc
     7981 agacgcaaaa attgatccga ctcatctaat actttgactt ggacaccttt ttggatggca
     8041 tcgaacataa atatctgagt agaaagttcc atttcgcgaa aaccggccaa ctgataggga
     8101 cgttcatggc tttctttata atattgatgt cccaatgcca cagcaacttc ttgctgactc
     8161 gatagctgtg cttcttgata gagtttcgct gctaacgttt gttcaggatg atccatttga
     8221 gtcaatgctt cctctactaa ataaagagaa tccaaccaat ccaattcaac cagcatttgc
     8281 ttcatccctt caagaatttc tctaccttct tgatgaaatt ctgtttgatc tgatggttgt
     8341 tctagtgcta ctttgttatt tcgagtcgta ccttctgcta cccattggtc acagtcttct
     8401 ttttcttctt tccataacat gtacatcagg aataattgta agaaccgaac ttgctctgga
     8461 ttgataccca atcttgcata aggattcaaa tcgatattcc gcaattcaat ataacgaaca
     8521 cctgttgttg ctaaatctgc gactttcttt ccgcctcgga aacgaagcgg tgtataaaat
     8581 tctttctcct ccgaaagttt cccttcttcg atcattcgct cgatatctgc taaatactgt
     8641 ttcatagaag cataggaaac tttcacattt ggatgattcg tatagcccag cgcactgtta
     8701 cgaatactgc gaacaggttc ttgcggatgt gattcatcta aaaagtagtt cttctcactc
     8761 attggtgaag caccgaataa ataagtgatc atccagcgat agcgcataaa gtttctcgcc
     8821 gttttcaaat aaagttctgt tttgaattcg gaaaaatctt gaaattcttc ctgatggctg
     8881 aacaatgttc ttagcaactc atcgccaaat tcaaaattga aatggatacc gcttaccatc
     8941 tgttttcgct tcccgtattc ttttgctaaa taacgtcgat acaagacatc ttcgaaattt
     9001 tttaattttg caataatgat cgcttcgtct ttttccggta aggcaggtgg catacttaat
     9061 ggccagatca tctcttcttt cggtatagaa cgagcagtca catcataaac agcagcgaga
     9121 tactgaaata attcgggaat agaatcggta acaggagtga tcatctctgt ttgtgtttcg
     9181 ctaaaatctg tttggatata ggggtgatag gatcgatcgc caaagattgc tggatgatcg
     9241 gtttttgcta gatttcctgc aagatcaacc cgctgtcctt cccgttctat accaaatcga
     9301 gcttggtcga taaaagggcg cgcattgaca tgcaataata attgcttaaa attcatcatt
     9361 aagtgacctc caattcatat gaatctaatt ataatcaaaa agtctaagaa aaagaacgaa
     9421 attgtttgag aaccgcttac aaataagtat aaaaatgaag gcatcaaaaa cacagctatg
     9481 ttaaaaacaa tcattcaaac aaggagctgc aacttttgtc acagctcctg ctttttagac
     9541 atgggttatt cttcttttag taatcctttg aacaatccaa cgacaaaatc atttggatca
     9601 aacggctcca agtcatcgat tccttcacca agaccgacca atttcactgg taaatgcaat
     9661 tcattacgga tagcaagtac gataccgccc tttgctgtac catctaattt tgtcaaaaca
     9721 agaccggtca catctgttgt ttctttgaat tgttttgctt gaaccatcgc gttttgacca
     9781 gttgttgcat ctagtacaag aagtgtttct tggggtgctt ctggtaattc ccgctggatt
     9841 actcgtttta ttttttctaa ttcgttcatc aagttcactt tattttgcag tcggcctgca
     9901 gtatcaacta gtaaaacatc tgcctgctct atctttgctc tttccaaagc atcgaatacg
     9961 acagctgctg gatcgccgcc tgcattgcca cgaacaactt caacaccagc gcgttcgccc
    10021 cagacaacta actggtcgat tgctccagcg cggaaggtat ctgctgctgc catcaatact
    10081 tttttacctt cgtttttgaa ctgatgggca agtttaccaa tacttgttgt tttccctaca
    10141 ccattaacac cgacaaacaa aataactgtt agtccgtctt cttggatatt tagttcattt
    10201 acttcattga ttccttccgc ttcatagaga tccacaagtt tctcgatgat cgcattttgg
    10261 acttctgccg attttttcgc atttctgagt ttcacttctt gacgtaatgc ttcggtgatt
    10321 cgtatcgctg tttcaaaacc tacatctgca ccgataagtg tttcttctaa ttcttcaaaa
    10381 aagtcttcat cgactgttcg aaaattggca aacaactcat tcatccgttc accaaatgtt
    10441 ttacgcgtct tttctaaacc cttgtcgtac ttctcctgta cttcaacttc ttctgttttc
    10501 tcttcgccag taaaagcacg cttgatttta tcaaaaaatc ccatttgact cccccctact
    10561 ctaatacaaa tttttggatc actttggcta cgccattttc ttcattgctg gctgtcacga
    10621 aatctgctgc ttctttgacg aaaggcacag cattctccat cgctacgcct agtccagcgt
    10681 attggatcat cggtaaatca ttttcttcat caccgattgc catgatttct tcttgtttca
    10741 aaccaagatc ttttgccaat aaagaaatac cataagcttt tgtaacacct tttggcataa
    10801 attccaaaag catacttctc gttttgatga tctcatacat ttcatggaat tctgctggta
    10861 tttctttgat tttaggatct aattcttctt ggtcataagc gattactgcc ttattaagca
    10921 caaattcatc tgtaatatcg cttatatccg ctggttggaa ttgcagcagc ttattcaaca
    10981 cattgtacaa ggattgcttc cttggggcac taggcagctg taaaaccaca ttgtcagaca
    11041 aaacatctag tggcagatcc aattgctgag ccagtttgta taagcgttgg atatctgata
    11101 cttccatcac tttcttttcc atgatttcac cagtatcatt tttttgtacc aaaccgccgt
    11161 tgaaagtgat gctgtagtca ccttcatcta ccagtcctaa ttcttgcaga taatgcacca
    11221 tcgcagctaa ggggcgtcct gtacatagaa cgatcttgac cccttgctgt tttgcttgtg
    11281 ctaatgcttg tttattctca tctgaaatca gcttttgttc atttaataat gtaccgtcca
    11341 aatcgattgc tactaatttg atcattttcg ttcctacact ttcgtttcgt tgattttcgc
    11401 ctcttcattg atatcttcca tccgtacaga aacgattttt gatacaccgg attcctgcat
    11461 cgtcactccg tacagtacat ctgctgcgac catcgttcct ttgcggtgtg tgactacgat
    11521 aaattgtgtg tcatcttgga aatcgctcaa gtaacgacca aaacgtttca cgttggcttc
    11581 atctaacgct gcttctacct catctaagat acaaaacgga acaggacaga cacggataat
    11641 agaaaagagc aacgcgatgg ctgtaagtgc tcgttctcct ccagaaagca aacttaaact
    11701 ttgcagtttt ttgccaggag gttgtacttc aatctcaata cctgttttca gcaaatcagt
    11761 tggttctgtc agtactaatt cagctttacc gccgccaaac atatttggaa agacaatttt
    11821 gaattcttga cggatagctt caaatacttc tttgaatctt gtccgcactt catcatccat
    11881 ctcatccatc gtttcaaaca attggttttt cgctgctaac agatcatctc gctgtgttgc
    11941 taagaatgta tgtctttcac tgacttgttc gtattgttcg attgaattta agtttactgg
    12001 tcccagtttt tcaatctgtt cttttaagga tgaaaccttt gttctactgt cttcgatatc
    12061 ggttgtttct tgatagtctt gggaagcttt ctcaaatgtc agctgatatt ctgactgcag
    12121 ataggtcaaa tgattatcta aaatcatttc agctcggtct ttttggactt ctaatttgga
    12181 ttgttccgac agtcgtgctt tttgctcacg attacgctct gccaagactg cttcaagctt
    12241 atcgatctcc ttttgccttc tatctcgctg ctctttggct tttgccaact cggctttcaa
    12301 tgtttctcgc tgagcagata attcgttaat ttgtttttcc aaactttctt ctgtgacttc
    12361 atgatcagaa aagtctgctg tcaatgtcgc caactgcttt tctatcgctt cttgacgttc
    12421 ggttgcctct gctttttgga ctcgggcacc gcggagctgg atctgcagat gattaaactg
    12481 ctctttcaag acagcaagat ctgcttgttc ttgtgctttt tgactttgta cttgtgctct
    12541 tctggcttcc atctggtcgc tttcttgact taacgatttg atttcttcgt cgattttttg
    12601 tcgttgcgat tcaagatctg tttgtttgtc tttcaattcg ttcgtttgct tttcgtaatc
    12661 ttcgataaac tgctgcagtt cacgtgtctc aaaagttgag atttgctttt ctttttcaaa
    12721 acgttcaagt tcatttatga tgttttgcag ctgatttgtc gcttcttgtt cttcaaaacg
    12781 taattgttcg cctcgtgtac gcaaaacttc ttgttcttcg gctaaacgag ctgtttcttg
    12841 ttgcagctcc tgaacttttt tttcttgtgc ttggagttgt ttatctgctt cttcaaattc
    12901 actagtcaac tgtttcaatt cttgattttg tacaaacaaa ctgccttggt ttcctcgttt
    12961 attcgctcca cctgtcatgg atcctccggc attcatcaca tcaccttcta aggaaaccac
    13021 tcgatattga tagcggatcg tttgcgcgat tgcattggca ctagttaagt ctttggctaa
    13081 aaggatcgtt cctagcaggt tttgaaccac agtttggatt tgatcgggat aactgacttg
    13141 ttcactggca atccctataa acccttctac agctgctgct tgtgtcaata tatgagccgg
    13201 taattgtctt ggtttgattg tcgtcagtgg aaggaatgtt gctcttcctc ctctttgctg
    13261 tttcaaatac gtgatggctt gccgagcatc tttttcattt tcaacgatga catgctgggc
    13321 tgccccacct aaagctgttt cgatagctaa cgtgaaatct gcaggaacat cgatcagttc
    13381 agccactgct ccgacaatac ccgaaagctg ctgtttatgc tgcaagacca atcgaacccc
    13441 ttggtaaaaa ccaaaatagt tttcttggat ttcttgaagg cttttcttac gagcacgtag
    13501 ctgctggact tgattcatta attgatacat tttaggctgt tcttttgcta atgcttcttg
    13561 gaactttttc ccgttttgtt ggatcatttc taacttctct tttgtttcag ttaaagatga
    13621 ttttagctgc gcctgctttt gtgtcaattc ttctttttgt aagatcaatc ggtctactga
    13681 tgcctctact tccgattgct tggctaacgt ctgcttactt tttgctgttt cttgtacata
    13741 ttgacgttct agatacttca gttcgtttcc tacagctgct tgctcttgca tcaaatcaac
    13801 atattgatct cttagttctg ccagtaattc tttcgttgat ttttggtatt tttctaattc
    13861 ttgctgtgtt tttatgagac ttgcttcagc tttttggatt tctgtttctt tttctgctac
    13921 ttctttcaac aagctttctt gaagcttttc aaaatgttta accttttttt gtgcttctgc
    13981 taatgaagtt tgatattctt gcgagctttt ttgtgtatgc tttgtccgct cctgcaaaac
    14041 atctttttgt ccttctgtct gcttcagttt ttccgaaaga tctaataata cttgctgatt
    14101 tttttcgatc agacgatcgg cttgcgcatt ttctttacgc tgcttcgcca aaatggattc
    14161 ttgttcttgg atcgactcag atagtttgcc taattctaga ttgaatttcg caagctgtgc
    14221 ttgtttgttg tcccaatctt tttttgctgt cttgatttcg gcaaccatca aagagacatc
    14281 ggtttgagtc agtgtttctt ttagccgtaa aaactccttt gctgcctcac tctgggcggc
    14341 caaaggagtt aactgttctt ccaattcgtg gatgatgtct tggacgcggc tgaggttgtc
    14401 ttctgtttca aaaagcttct gttctgcttt ctttttccgc tgcttgtatt tcaagacacc
    14461 agctgcttct tcaaagatcc cacgacggtc ttccggttta ctactgaaaa tcgcttctac
    14521 ttttccttga gaaatgattg agaacgattc ttttcctaat ccagagtcta agaacaattc
    14581 ttggatatct tttaatctgc agctttgttt attgatgaaa aactcgcttt ccccagtccg
    14641 tcgataacgg cgggttacac tgatctcatt gtactccaat ggcagataat gatcactatt
    14701 gtctaaaatc actgtgactt ctgcaatatt cagctgctta cgggtatcgg aaccggcaaa
    14761 gatgatatcc ggcatttttc cgcctcgcag acttttagca gattgttctc ctaataccca
    14821 gcggatagct tctgtaatat tgctttttcc actgccattt ggtccaacca ctgctgtgac
    14881 gctattttca aagtcaatga ctgtcttgtc tgcaaatgat ttaaaaccgg ctatttcaat
    14941 tcgtttcaaa tacaaagctt tccctcactt acttttacag tgctgccaat gcattttccg
    15001 ccgcagcttg ttctgctaat tttttcgatt ttccttgtcc agtgccaatc aattgatcat
    15061 ctacataaac ttcgatccaa aatactcgtt catgagctgg accttcttca ttgatcaatc
    15121 ggtattcgat tgatacatca cctgattttt gcaagacttc ttgtagtttt gttttatgat
    15181 ccatctcatg tgaaaaagca cctgctttga ctttaggaaa aatcactttt tcgatgaatg
    15241 catgggtcgt gtcgaatccc tggtcaagat ataacgcgcc taaaaaagct tcgaataggt
    15301 cgcaaagcaa tgctggtcgc gtacgtcctc ctgaattctc ttctcccttg cccaacaaaa
    15361 catattgatc aaagtgacat tctttggcaa atttcgacaa gctgtcttcg cgaacgatcg
    15421 cagctcgtgt ttttgtcaat tttccttctg gcaaatgagg atataaacgg aataaatact
    15481 gagaaaccat cagttctaaa acggcatctc ctaaaaattc aagtcgctca ttatccgata
    15541 attgcagatt gcgatgctca ttcacatagg atgaatgggt aaaagcttgt tcaagcaaat
    15601 ttagatcgtg aaaaacgatg ttatagtttt cttttagttc ttttgtcagc tggttgtcca
    15661 ttttccacat tccttttatg gatatttttt cgatacttcc ttattatact ggttttttgt
    15721 caaatttgaa atggttcggt caaaaggtaa gtaaagcttt aataatccta gtgattgacg
    15781 agaagttgtg aaaaattcgt ttagtttcga gtatgaagaa aaaattttgt gaaaatggct
    15841 ttccatattt ttgcataaat ttgacttaat accgagaaac tggactttga acaccgttta
    15901 ttggagaagt catgaaatca gcgccttgac ctgaatatta gagaaataaa cagaaaaata
    15961 tcaaaaaacc gaaaaaggac atcgcccttt ttcggtcatt tttagtgatt atgaacgtga
    16021 atctgctgta acagaaacat ttgcccatgg gtttggtaca tcataagacc aatcccagtc
    16081 tttgacacgg tcgttgactg gcaatacttc attacggaac aacgttggaa tcgcaaatgc
    16141 ttcttcaaat gcatattgtt gccatttttg atatgcttct ttttggtact cttcatcaaa
    16201 tgctttgctt gaactgatat cgttgatcaa ttttgtattt tcttctgatt cataacgagt
    16261 gtagttgaac gcagcatttg gtccccatag tccacttgga tttggatcag ttcctgtatt
    16321 ccaagcagct tggtaaacat caatttcagg atcatcattt tctaatttat catagaaaga
    16381 attgaattcg atcagacgac ctgttgtgta ttggacatcc aaaccgattt ctttccactg
    16441 ttggatatag taatctgcca atggttgcgc tgtttcccca ccggacatcg aagcaaagtt
    16501 gattgttagt ttatttccat ctttgtcttc tcttaaacca tcgtcatcca catctttgta
    16561 gccagcatca tctaatagtt ttttcgcttt gtctagatct tgtgtgaatc ctttcatctc
    16621 atcatcatgg tatgactgga atactggagg aatcaatgaa gtcgcattgc ttctcaaacc
    16681 tgcatagaaa cgctcaccta ctgcattatt atctaatgca tagcccattg cttgacgtaa
    16741 tgatttatca gccatttttg aatctggatt gtattccaca ctctttgtat ctgcattcca
    16801 tttacctaac ttgaatccta gataagtata agaagtttgt tcacggccta agtttgtata
    16861 gccagcaatt tctttatagg tatcgtaagt atctgttggc atttggaaaa tcatatcata
    16921 taatttagag ttcaatgctt cagttgcact tgccatcggt acagatttca tcgtgatctt
    16981 tttcaattta ggcgtaccat tccagtagtg ctcgtttggc aagtattcta ctgattctcc
    17041 tgcaacgata ttactcatgt aataagggcc aaaagtcact ggatttttac gtacttgatc
    17101 gcttgattcc atatctttga ttgctacacc ttctaacgta tgttttggtg ctgcatatga
    17161 ccaaatacca ccacccgatt gaagcatgct tgggtttact tctttatatt cgatttcaac
    17221 tgttttgtcg tctatttttt tgattccgga gattgtgtcg gcacttccat catgatattc
    17281 ttccatacct acgatgttac ggaaagtgtc gtcataacgg ataccagtgt aatctttatt
    17341 cccaatgatt tcgtaaggga aaatcacatc gtccgccgtt acatcttcac catctgacca
    17401 tttgacgttg tcttttactt tgattgtcgc ttttttcgta tcttcatcta gatcaagcgt
    17461 tgcagctccg tcgtctgtga ttctgaaatc gtcatcgttt gcaaatagcg gttcatgaga
    17521 aggagccata aaagctgcat catatgaatc ttggtaaaac tcccactgga acaatccttt
    17581 aaactgactg tccgttgcga ccgcagcttc taattcgccg tcttcgattg catcatcatt
    17641 attttttgtt gcaattggaa aagttaattc ctctttgtta ttgccgttgc ctccagagtt
    17701 gtttccagag gtatctgtac ctccgccccc gcatgctgca agaacgacag cagacatcag
    17761 cgctgcaaat ccaaaaatcg ttttcttcat tcctatttcc cccttcgctt ctatactagc
    17821 ctaaccgttg gcgcgcatct gcagaacgct tgaatgcttg tccaacataa ttgatactca
    17881 acatcaaaac taaaataagg attgatgcgg gtaaccagat ccattgccga ttcactaaga
    17941 cttctccgtt acttgcataa ccgatcaatg tacctaaact aggtacattt gccggcaagc
    18001 cgaaacctaa gaaagtcaaa gttgtctcga taccgatgtt tgccgcaaag ttcatcgtca
    18061 aattagtaat gattaatgaa cttagattcg gcatgatttc gcggaacatg attttaaaat
    18121 cattggttcc cattgttttt gaggcactga cataatctct tcttgcttca gataatgtct
    18181 tgcttcggaa taatcgggct ttagctaccc aataaaatgc actcatgatg aatataaagg
    18241 accagacatt gtatctggaa ataatcgata cgaagacgat aatgatcagc atgatcggta
    18301 gaatcatgat aaaatcaacg atccgcatca agatgttgtc aatgatccct ccgtagtagc
    18361 cagagatgat ccctaatcca accccgatga tcgaagtaag gatcgtgatc gcaaatccga
    18421 tcgtaacaga gtttctagca cctatgatca attgtcctaa tacatcacgt ccgccttcat
    18481 ctgctcccag aatgaacttt tgtccttgtg tcgaaactgt tcctggtgca gcgtatttgt
    18541 cgaagatact aacatacatc actttgtctt gatcggtcag catcgcaccg ataaagatca
    18601 ctaataataa aaccactagc aaacctaatg aaaaaatagc aattttatct tttttaaatt
    18661 ctcgagcaat cattcgtatc cccataggag gaatactttc ttgaactgct gtattgtttt
    18721 tttcactcat cgatccattc tcctttctga ttggttttat tgaatccgaa tacgcgggtc
    18781 aacgatactc atgatgatat ctgaaagtaa tgtccccact agcgtcgcga ttcctaaaat
    18841 caatactaag gatgtgatca cggtatagtc ccgttggccg atggaatcaa taaatagttt
    18901 tccgactccc ggataaccaa aaatcttttc gataactaca gaaccagcaa tcaacgaagt
    18961 gatttcatac cccatttgag aagccatcgg caacgctgca tttcggaaaa tatgtcgact
    19021 gaatacttta ttcgtcggta cccctttgga acgggctgtt ctgacaaaat cgagcgattt
    19081 cgaatcgatt acttcacttc gcagatattg gattgtgacg gcagtcgcca aaaatgcttg
    19141 ggtaattgat ggtaacagaa tatatttcaa acggtctatc cattgagtga taaatccacc
    19201 ttgcaaacct agtgtttgtg atccacttgt tgggaaccag cctaaacgat aaccaaaaac
    19261 aaatagcatg atcaagccga agataaataa aggaacagca aaactaaaga agttgtagat
    19321 tacgacaatc ttatccaaga ttgaattttg ataccggcca gcaagcatcc ccagaggcac
    19381 agcaatcaaa taagtaagga caactgtcaa gatcgataga tataaagtgt tgatgatccg
    19441 ttcgccgatc aagtcagcaa cagagcgttt gtataagaaa ctttgaccaa aatcgccttg
    19501 caaagcattt ccgatccaac ggaagtactg gacataccat gggtcattca agccagctgc
    19561 ttgtcgcatc tgttcgatca ctgccggatc ctgatttgga ttgatcaaac cggtaaatgg
    19621 atcccccggc atcgcttggg ctaataagaa gatcagaaca cttaaaataa taacttgcgg
    19681 gatcatcaat aaaacacgtc gcagaatcgt tttccacatt agcttgcacc tccatctttc
    19741 aacgccactt tgtgtgtagt tgtgatcgta cgcaaatcgt agactcgacc agtcgcatca
    19801 tagtattctt tttggttttg gatatactct tgttctactc ttcgtcgatt ttctttgtgg
    19861 ctttccctat tttctacatc gatttgcgga atagctgaaa gcagacgttt cgtatagata
    19921 tgtcgcgggt cattataaat gtcttctctt gtaccgattt ctacaaatcg acctttgtac
    19981 ataattgcga tattgtcgca catgtgtttt actacaccta aatcatgtga aatgaataaa
    20041 tagctgaggc cgtattcttg ctggattcgt ttcatgaaat tcaatacttg cgcttgaaca
    20101 gatagatcca gagcagatac aggttcatcg gctacgatca gttttggatt ggtagcaaca
    20161 gcacgggcta ctcctagacg ttgacgttgt ccgcctgaaa attcatgagg atatttatat
    20221 agcgcatctt ccggcatccc aacgatatct agcagtcctt ttaccttttt cttttcttct
    20281 tgatcgctta accgttcaaa atttcggatg ggttcagcaa tgatatccaa cactcgcttt
    20341 ttaggattca aactggacat tgaatcttgg aagatcatct gcacatcctt gttataaccc
    20401 atttttttgc ggttgcttcg cttcgttaca tcttttcctt catacatgat ttgtccggct
    20461 gtcacctttt ctaatcccac aacggctttt cccgtagtag attttccaga gccagattct
    20521 cccactaaac catatgtctt tcctttttcg ataataaaat ccacaccatc tacagcatat
    20581 acatgatccg ttactcgatt gaagaatcca ctgcgaatag ggtaatgaac tttcaggtct
    20641 ttgattgtga ttaattctgt catctacacc tctccttttt cgtctctaaa gtggaaatgc
    20701 ttatagcagg agcaacgtac caagtggttc ggtgcgactt catgaaggat aggctcttct
    20761 tcatgatcgc ttgctggtat ccatggaata cgtggtgcaa accgacaacc tttacgaggc
    20821 atgtttttca gagaaggaac gacaccttcg atcacatgga gttctgcatc atgatttcct
    20881 tcttgtggta tagagttcag taaagagcgt gtataaggat gcttaggatg atcaaacagt
    20941 tcttttacac ctgctacttc aacgaactgt ccgccataca tcactgccac tcgatcagcc
    21001 atttctgcta cgactcctaa gtcatgagtg atcaagatga tccctgtttt tgtttcttcc
    21061 tgaaggtctt tcagtaaatc aagaatctgt gcttggatgg ttacatctag ggctgtcgtc
    21121 ggttcatctg caatgacgat tggaggtttg caggcgattg cgattgcgat gatgacacgt
    21181 tgtctcatac cgcctgatag ttcatgggga tattggcgtc caactctcgc aggattagga
    21241 atccctactt gttctagcaa ttctagtgca cgagcttgac gctgttctgc attcatcttt
    21301 gtgtgatagg aaagtccttc tttgatctgg tcttcgattc tcatgagagg atttaatgca
    21361 gacaacggat cttggaaaat cattccgata tcatttccgc ggattttatt atagagcgtt
    21421 tcgttcaggt cgattaaatt cagatcgtta tataaaatct cacctgtaat ttttgtgtta
    21481 ttaggatcaa gtaatcccat aatcgtagta gccaaggtac ttttcccgca cccagattcc
    21541 ccaacgattg ctaaaatctc gtttttgtct aaagtaaatg aaacgtcatc tactgcatcg
    21601 taatagtcat ctttgagtcg gaatcctgta tgtaggttct gaacttctaa taattggtta
    21661 tcggacaatc gaatacccct ctctgcttcc taaatggaag tttttcttaa taaaaaagga
    21721 cattacacag aataaagcgt tttata
//
LOCUS       DAXWOO010000035        21098 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.035, whole genome shotgun sequence.
ACCESSION   DAXWOO010000035 DAXWOO010000000
VERSION     DAXWOO010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21098
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.035"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..376
                     /locus_tag="ACGE70_001020"
     CDS             <1..376
                     /locus_tag="ACGE70_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tra protein"
                     /protein_id="HFF8692670.1"
                     /translation="INSNNKYACEYVLSFEREMRKMRAGVYFATQTPEEIIPKDSGSE
                     VADVIRNIFNLCTFKCFFNLDSALLEDIKKVLGNTITDTEIMLLPELEVGQAVVQTSS
                     EDTYMINFDPYPEQIERFDGGQ"
     gene            398..1534
                     /locus_tag="ACGE70_001021"
     CDS             398..1534
                     /locus_tag="ACGE70_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HFF8692671.1"
                     /translation="MEQNKNNNVASTLARQGMKQVSKKIGLALKIKIGVVIGLITLVG
                     LLVIGSLATLQGTQTATNTGGAEVIGGNKPLSPEVQKWQKTVEKYAEKYGIKELVPYA
                     LAIMQCESGGKLPDLMQSSESAGLPVNTLQYEESIDQGLKHLSNVYNIAKSYGMESNY
                     DGIVQAYNYGIAYMHYLGKNKKTHTIETAEAYSKNVVAPSLGNTTGQTYSYVNEVSIK
                     YGKTYLYLNGGNFYYAELVKQYIGGGSGNPANPSEFWGKMKTEMEKYQGWPYVWGGKN
                     PTTGFDCSGLTAYLYQFSAGITIDSYTVTQYEATEAVSIKEAKAGDLIFFRGTYGAPD
                     FISHVGIYVNETTMYDSNSSGIGYHNWQDSYWQSHSPEIRRVKK"
     gene            1549..2199
                     /locus_tag="ACGE70_001022"
     CDS             1549..2199
                     /locus_tag="ACGE70_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFF8692672.1"
                     /translation="MIKRKNDNKWKYICGVLIIILVLGGLLSQLHTSNIQAKNKKLDQ
                     EVTQLEEQNSKLSKNISDLNANVEANLTNDDGSELNKQIAEVAKKFVELYPVYDIEKV
                     EEKKADLEKIATSSVADSIVPDDMITTSKKTLESKSTTQKGEAYSSDPTFKSKYKDST
                     LYTNYVNANTINYFAEVNYKTESSSGDTENTVYMLFEVTNKDGQVLVDNYEIKYLK"
     gene            2213..3109
                     /locus_tag="ACGE70_001023"
     CDS             2213..3109
                     /locus_tag="ACGE70_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692673.1"
                     /translation="MNKKYSIHNKKEPNKKNKAQIDKVYKKTKGVKLYLGAILGFLAI
                     GMMIVFSPYVMGKSYDFETVKMSSAQNVTSSLNMYVDDMEINREAGLFKLVLRYQDDT
                     GTKSLSNIKSDYKLNFITNKNNEDVKTKVVKLSDEYTVIYYQNLPKDFGVISVTVNPR
                     YIYPELEPSNDLKEKEIKFYAVDKDIKSNAKLEVESTSDLQKDNFKYQIKAIENSIKD
                     EKKSIEKMNLANKVVQKDIDKANQNLEFQTLDEQTDTKNEISSMQTTIENNKSSIEES
                     ENKIKQFEEKIKQLEKSASRFE"
     gene            3145..3375
                     /locus_tag="ACGE70_001024"
     CDS             3145..3375
                     /locus_tag="ACGE70_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692674.1"
                     /translation="MCNSIVTIQEKAEEELMRIEAEYKEKRKAIKRKKNAELKKYKEE
                     QALKLGMMLCKYYDCYDINEVLKKANLNQFCE"
     gene            3726..4022
                     /locus_tag="ACGE70_001025"
     CDS             3726..4022
                     /locus_tag="ACGE70_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692675.1"
                     /translation="MDVSIRGIDPQYVKEIDKKVKKIQERTGKKFSRNDYFLAIIRQD
                     SEIDLIDYKKSEFDLVVEKLLLSNEQMVESISELTSVYERLFNFLISERSDLNK"
     gene            4019..4486
                     /locus_tag="ACGE70_001026"
     CDS             4019..4486
                     /locus_tag="ACGE70_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692676.1"
                     /translation="MNYPYFKVSATEDVKEKFNKCFEVNKSVFQTKANMFRYVVSNLP
                     MLTKANQNFRDPEKEELAKNLEEAKLKISELETIIELQKQQLENFNKKEDSIHKDYSE
                     QLNSIEESVYKLLINSGLASKELEEERAEPEKESSRLGDGTMFSSPSLRFTTD"
     gene            4568..6088
                     /gene="mobP2"
                     /locus_tag="ACGE70_001027"
     CDS             4568..6088
                     /gene="mobP2"
                     /locus_tag="ACGE70_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobP2 family relaxase"
                     /protein_id="HFF8692677.1"
                     /translation="MTEHIFNRKSASIILKAKFETGKNKKKNHMVELQKFVDYISRQE
                     AIRQDKLDHDYSEDELNELARIEKALEKIEQETDQPVIKDLREMDKYIDYMTRKKAIL
                     ENIDNEIVNGAFSNTKRYITKKDISKIKESVIEAKNNGSVMFQDVISFDNDFLVREGY
                     YNPETNELNENILYEATKSMMGKMQEKEELVDPFWFATIHRNTEHIHIHVTAMERKNT
                     REIMEYDGVLQARGKRKQSTLDDMIFKFGSKILDRKNEFEKISKLRKEVPLELKQSVK
                     DSLIQLYVENNSRYDKELVKYLKELKKEIPSTTRGYNELPEETKEKIDEVTNYMTKDN
                     PKRKEYDRMTKEIDELYQTTYGKRYNPQEYYLNRKKDFNARMGNAVVGQIKLLKQSEE
                     KNLKSNELKNLLDGKKLNVKFDREKPEFKTKYAKQSYDDFQKRLEERQLAREKNEVHF
                     KKISEKFKDKKLIRKIDRVINDDLNMYRAKNDYEVAQRMIEQEKMRQAHEFEIGMN"
     gene            6110..6439
                     /locus_tag="ACGE70_001028"
     CDS             6110..6439
                     /locus_tag="ACGE70_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692678.1"
                     /translation="MKNFDIVCSNTKNIYLRELLNSDSETIEDVKKIIVLFEKENMEL
                     ENWGLFEIPISGNYCFYNWKTEDDVAFANYFFDKNYFSPLYIDKHSNEQVASSIKEAI
                     KLERVRK"
     gene            6441..6704
                     /locus_tag="ACGE70_001029"
     CDS             6441..6704
                     /locus_tag="ACGE70_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692679.1"
                     /translation="MIDFRTVMDKLSNGDFRVLIELYLNVTSIVKEFLYSNPKIALVL
                     VAVLIVFAVISFIKKMIPLLIIGAVLILGLYFPEVLDQIKNIF"
     gene            6886..7638
                     /locus_tag="ACGE70_001030"
     CDS             6886..7638
                     /locus_tag="ACGE70_001030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692680.1"
                     /translation="MYYQLLYDKIIDAETFYYKEKNEWLNGLSLYLRFDPIFYVENKQ
                     KSKEQMIQLKNERSLLNRLVRVSYDNQTRKLSFHDHEEIKGKYRVAKYYKLVTDEEYS
                     GWCEEEKISKEEFYFLIQHYGQHFHNDFSLQSCSYGWHEIESLPEGTIVQNSYPKHLN
                     ETTKRLEEIVFEEKKRHEQLLSVKKGIMKFEYRSECLEFIKRIELLENHKDFLLNKEQ
                     FDQEEVMESYVQLYTEWIRFNRFMSQENLYTA"
     gene            7941..8918
                     /locus_tag="ACGE70_001031"
     CDS             7941..8918
                     /locus_tag="ACGE70_001031"
                     /inference="COORDINATES: protein motif:HMM:NF022432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HFF8692681.1"
                     /translation="MEDKKYSKIIKKIKGLLAIAKDENQDEESQSAFVLAQRLMLQYK
                     IDKNDVFDTEDEIEPIGEESVTIYKKLYWWERSLGSIIAENFRVKMFYNSKKEKGERK
                     SKSKIVFYGFGSDLELAKEMYFLAYEVIVFHTNQYIKNWYQDTDMKRSRYITESVKSS
                     YISGFLVGLQKRFEEQISVLAERYEVMVLIPKAVEESFDKYSKDFSSYSTNTPPVSVD
                     HAYQEGVREAKKIDLTKSTVDTDYSSLVGRIIRFNQGVTRGLIAEITSVSDDLLNLLV
                     MNCVSEYASEDNPSFYHWKVDKEYDYEILSFNDPMSKKFKKNKVDIMEN"
     gene            9070..9936
                     /locus_tag="ACGE70_001032"
     CDS             9070..9936
                     /locus_tag="ACGE70_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692682.1"
                     /translation="MKKLRVIATIPMIFSFALLLSACGKEEAGAKSNEAHNISFIDSA
                     EQKDSRCWLVVETSSKNKTPIDKNEYVKAVIQTKDGKAKVFKTDMEQLTLEKVSKMSD
                     EEIIEQALNADKQKFNDKKKSDIDSYNATLDSVKTELENEEKQEFQNDQKIADFQDSI
                     PKIQADIEEIDGVQYSEPEWEKVSATIKMDNENKSPSMETVKLSYKYLDSPIWNLNSF
                     GEVSSEGELSYEPTSMDLKLSIPVATTKVLDNFYSGYSNDELDQLLLTKVASKDNKVQ
                     LDSKGKNVSIVE"
     gene            10091..11917
                     /locus_tag="ACGE70_001033"
     CDS             10091..11917
                     /locus_tag="ACGE70_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFF8692683.1"
                     /translation="MAYKKGNWKPKTKEQKQKELDDLIKASNSKIEQYQSNPEDMIEF
                     AKFMSKIHNYSPLNLSLIDEGFEGAIAVASYDGWKKLGFQVKAKEKGIGIYAHAPVTV
                     FIDENGEEKTLKEATSEQKEKIKNGELRSKKIKHFKKGYVFDISQTNATEADLPKIFP
                     NRVWNFNIDGELSVSQLEKGVNHLAESMNISIKDMNESGRGEMGSARGEYIQYVDGRE
                     EIVLNSRNSRTQNLTTSIHELAHKKLHNKNEHGSSYSIPIKEFQAEMTSFIVCHNYGI
                     DTSDFTIPYIANWTKNNEKIDPKNFRQIMQEVRETSIDFISTIDKKVLEEQQTAFNAL
                     KNSFVPNENKEQIQQKSDLEFHNFYDSTINDPDRTIHGSYLEAMVDYYRGKYGENSIE
                     LTHSNYDVTAKIGENYVTYYSDTKQFDVCDVYGVVFENPSENKNDRLLLDRSPENCDY
                     VEKFVVESLKVCENHELKNDTLKLSEPLRSVVINEAVYNLSENANNYYNEKCWQEEIE
                     NQTNINDVKELNSCMTAEDFYESHKQEIENTITDFESVSGTNLRDTIDNESYKEVASV
                     VAYKLVATKVKYEMDEGTFKIPENSNLEKPTVKTAERSFEME"
     gene            11925..12998
                     /locus_tag="ACGE70_001034"
     CDS             11925..12998
                     /locus_tag="ACGE70_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692684.1"
                     /translation="MSNEQNYETGGTYRLFHGSMNKFDEFSLDHKGLNGTANGFGVYL
                     TPNKEMATMYAEQGSNGNNGFVYEVNVELEKPISMNERTITDEELSKVIDKLQESNDI
                     LNDYNDVSYYGEKVVKREVIASLSENENDVDLINDIGNTIGDHEATAKAFYDVGKYTH
                     IIAEEQLRQKDVVIVVLQPDKVKIETIHDLNEERNSLESSEDMTDKFVGHKVSEVPIR
                     EVVIKDFEQELETTNYYDKQAWQERIEYLENMEEPVSRLALDSDINEFYDSHKEEIET
                     KLTNLSEKLEIDKEELLGFPVDTSSTEYKKATSIMAYNGAMREIYNEIENDTFEIPPV
                     SEEMENKILKLTNFENTTKFEME"
     gene            13007..13267
                     /locus_tag="ACGE70_001035"
     CDS             13007..13267
                     /locus_tag="ACGE70_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692685.1"
                     /translation="MIEQENRNFLETQGEYRQINNSHFVICEDVQIAFKMALKYGKSI
                     RKKIIREKKSKKIGFWELSLRDGLNFIFCTEDYYEKYKCTLS"
     gene            13304..14308
                     /locus_tag="ACGE70_001036"
     CDS             13304..14308
                     /locus_tag="ACGE70_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3991 domain-containing protein"
                     /protein_id="HFF8692686.1"
                     /translation="MDLKKAKKVSIVDFCKQNGIGLKQDSREYYRLEDHDSCVISDRK
                     NLFKWNSKDLGGDIFDFIKAYYDCSFKEAKQKLEDSDYEVHSFNTKVKKAPYQYQKEN
                     EVPISVAKSYLVNERRIDTALVEDFLSKGLIKQDKKGNVLFLWKKDQEIVGCTEQGTR
                     KFFHEGKQKDVTWKKIQENSKEYSGFNVTFGQPDKMYFFESEIDLMSYITQKPEKAEN
                     ATFVSMNGLKVGNVLHGVTEHYNKFKSLPSETVLCVDNDKAGRTFIETEFKEKGIGRE
                     GIGISEVKVDVPLREGFDWNDNLTRGKEAFKRFKLEDVSKIVNFTHKLSLNTPVYEME
                     "
     gene            14333..14689
                     /locus_tag="ACGE70_001037"
     CDS             14333..14689
                     /locus_tag="ACGE70_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692687.1"
                     /translation="MNDTIAILSDSLIDLDKTSTDYMIIEEQLKNKNPEFDIQEKSLR
                     IELPKFKQKKLITILFEIIERYGLTYYQIWTDKKDGQYTKGVSIEAVREDDQVSIKAV
                     QPLMGYNEELLNCLNV"
     gene            14851..15042
                     /locus_tag="ACGE70_001038"
     CDS             14851..15042
                     /locus_tag="ACGE70_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692688.1"
                     /translation="MEQTRMFWSDRMQDHLDCIYKTREQLNKLDTVRYYFTYFSENDE
                     QRWDIFHDGNYEEFFYTES"
     gene            15151..15369
                     /locus_tag="ACGE70_001039"
     CDS             15151..15369
                     /locus_tag="ACGE70_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692689.1"
                     /translation="MVNKKETFKVGMEVQGVVIGSGELIEGEVISLLANVAVVKAIGK
                     APVVMKKCSMMCLDGLNNPITQRFENVI"
     gene            15406..15723
                     /locus_tag="ACGE70_001040"
     CDS             15406..15723
                     /locus_tag="ACGE70_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692690.1"
                     /translation="MKDYASRKFIHLEPMKELVINGNEYNDYKSLFVKEQPKGGIRMK
                     NLPDFRFIEVNNLGIIEDYLDKLDEAIKQSIEKNGEDHILTVVLKDLKDRLEAEAEYF
                     NKD"
     gene            15734..15931
                     /locus_tag="ACGE70_001041"
     CDS             15734..15931
                     /locus_tag="ACGE70_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692691.1"
                     /translation="MRAMTEAVKELKKMYPDVLNMTVDDFHEALKNAESEEERTFYLT
                     LSSFVTRVDQKKVINQKDFKI"
     gene            15928..16896
                     /locus_tag="ACGE70_001042"
     CDS             15928..16896
                     /locus_tag="ACGE70_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFF8692692.1"
                     /translation="MSLENEKPYYVVVGGVNGAGKSTIYQVQNLKRKELDTDRINADE
                     ILRSFGGNWKKQADINKSMREVLREIKGHFKEGKSFHHETTFGGNPYPDKMRLLKAKK
                     LGYESFLIYIGLDSPERAIERVKIRVAKGGHGVPEDVIRKRYEFSLRNLAELEKYFDN
                     VSIYDNSDEFSYIYVKENHTVVANNTQEYNWVPKAIQEKNAEIPISKQIIKDISQEGL
                     SPKETLERIYLASDDFISDRFFKAEQVDDFYMNHKNEIKERILAISFSTGVPEDELVG
                     QDKKRNSCKLAYINSVGELLGKTDSELSGMEVNRDRSIKKTDFEIE"
     gene            17140..18312
                     /locus_tag="ACGE70_001043"
     CDS             17140..18312
                     /locus_tag="ACGE70_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692693.1"
                     /translation="MTKKIEVYVDGSQEERDSFFGFFWKNSREYVKVLNMVYRQLMFE
                     ELIKDAIKEMDQGYVEREKSIQNRIEKLAYTDPIDQKKLDKAKAELSKLRNEKNKEAR
                     EILTQAIGLKSQTRTRDVAKKLDFDFGDFVDSANMKASKDFGNDFVGIVTGQRTPRVY
                     RNKSKYGLLPFRGRDIKIHKADKFYISLPKGFRFKVNLGSKPRKARDSALTLGYIMDG
                     KYKLNQSNLIFEGNKLFFNLTISFEKDYHKLEFKEGSELTIKFTDLCSGECLVNNKSV
                     IDFGDSKYISNFKNKIKAMKSREQSRGVFSKGGHGRKRKLDQDRWEAIKKRESNFVKT
                     YNNQCSAKIVQTAIRNKAQIICISEPDKSYGEWAYYQLKEQIINKGKRYGIEVKSV"
     gene            19145..19756
                     /locus_tag="ACGE70_001044"
     CDS             19145..19756
                     /locus_tag="ACGE70_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFF8692694.1"
                     /translation="MLVGYARVSSDDQNLERQLEEFNRVGVEKVFAEKKSGRNTAERP
                     EFNKMLAFVREGDTLVFESLERLGRNSDEILDTVSFIDKNGMSLMVLNLPILDTKFGD
                     PNLEKLVRSLVISIFSWTAQNEREEIKRKQKQGIAIAKRKGYYKGKPYEYSENAKNPQ
                     KRFIYNEIVRMLGEGLPIKKIAEKTGTNRNLVYRIKKRISESG"
     gene            20000..20230
                     /locus_tag="ACGE70_001045"
     CDS             20000..20230
                     /locus_tag="ACGE70_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692695.1"
                     /translation="MNQPRRKRLESISEKISELKDLLEEIKEEEEEYKENMPENLWGS
                     ERYERAEEAVGQLEEADDYLDQVIGLIEEVIS"
     gene            20284..20637
                     /locus_tag="ACGE70_001046"
     CDS             20284..20637
                     /locus_tag="ACGE70_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692696.1"
                     /translation="MTQNYIDNWRLFSGKLMTKDSIAEMPESLEKEHILKVREMLHKE
                     GFNADLFRVGELPIEGVYYFERAVSENGVDYFSIIVEDDGSLTPQYTTLECDESGFNT
                     FPCNTIQESIDKHEC"
     gene            20665..20973
                     /locus_tag="ACGE70_001047"
     CDS             20665..20973
                     /locus_tag="ACGE70_001047"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692697.1"
                     /translation="MKLEIKEVVCDWGIYVDGQTYPFMIFNSKANAQEIMRIMELDNK
                     HERFDEVNKMENECEAVEYHKTCANNKYILGAMGLGKTASIKQVYEESQENCVYENSN
                     "
BASE COUNT         8506 a         2389 c         4112 g         6091 t
ORIGIN      
        1 tatcaattca aataacaagt atgcttgtga atatgtacta agttttgagc gagaaatgcg
       61 aaaaatgcgt gcaggtgttt attttgcaac tcaaacgcct gaagaaatta tccctaaaga
      121 ttctggatca gaagtagcag atgtgattag aaacattttt aatctatgta catttaaatg
      181 cttctttaat ttagattctg cattgttgga agacataaaa aaagttttag gaaatacaat
      241 tactgataca gaaattatgc ttttaccaga attagaagta ggacaagctg tagttcaaac
      301 ttccagtgaa gacacctata tgattaattt tgatccatat ccagagcaaa ttgaacgatt
      361 cgatggtggt caataataga aatcggaggt gaatttaatg gagcagaaca aaaataataa
      421 tgttgcttca acactcgctc gtcaagggat gaagcaagta tcaaagaaaa taggtcttgc
      481 tttaaaaata aaaatagggg tagttatagg actgataaca ttggttggcc ttttagttat
      541 aggttcttta gctacattac aaggaacaca aactgcaact aatacaggag gtgcagaagt
      601 cattggtggc aacaaaccgt tgtcacctga agttcaaaaa tggcaaaaaa cggtagaaaa
      661 atatgctgaa aaatacggaa ttaaagaatt agtaccttat gctttagcaa ttatgcaatg
      721 tgaatccgga ggtaaattgc cggatttaat gcaaagtagc gaatcagccg gattgcctgt
      781 aaacacactt caatatgaag aaagtatcga tcaaggactt aaacatttga gtaatgtgta
      841 taacattgct aaaagttatg gcatggaaag taactatgat ggaatagttc aagcttacaa
      901 ttacggcata gcttacatgc actacttagg aaaaaacaaa aaaacacata ctatcgaaac
      961 ggctgaagcc tactctaaaa atgttgttgc tccaagttta ggaaatacta caggacagac
     1021 ttattcatat gtaaatgaag tatcaattaa atatggaaaa acatatttgt atttaaatgg
     1081 tggtaatttt tactatgcag agttagttaa acagtatatc ggtgggggta gtggtaatcc
     1141 ggctaatcct tcagaatttt ggggaaaaat gaaaacagaa atggaaaaat atcaaggttg
     1201 gccttatgtt tggggcggta aaaatccaac gacaggtttt gattgtagcg gattaacagc
     1261 ttatctttat caatttagcg ccggaattac aatagatagt tatacagtaa cgcagtatga
     1321 agcaactgaa gcagtatcaa ttaaagaagc aaaagctggt gatctgattt tctttagagg
     1381 aacatatgga gcgcctgatt tcatttctca cgtaggtatt tatgtcaatg aaacaactat
     1441 gtatgacagt aattcaagcg gaattggtta tcacaattgg caagattcat attggcaatc
     1501 acatagtcca gagattcgta gagtcaaaaa ataggaggaa gaaaatagat gattaagaga
     1561 aaaaatgata ataaatggaa atatatttgt ggtgttttaa ttattatttt agttttaggc
     1621 ggattgttat ctcaacttca tacaagtaac attcaagcaa aaaataaaaa attggatcaa
     1681 gaagttacac aactagaaga acaaaatagc aagttaagta aaaatattag tgatctaaat
     1741 gcgaatgtag aagcgaactt aactaatgat gatggtagtg aattaaataa acaaattgct
     1801 gaagttgcta agaaatttgt tgagctatat ccggtatatg atattgaaaa agtagaagaa
     1861 aagaaagctg atttagagaa aatagctact tcttctgtag ctgattctat cgtgccggat
     1921 gacatgatta caacgagtaa aaagacttta gaaagtaaat ctactactca aaaaggggaa
     1981 gcatattcaa gcgatccaac atttaaaagt aaatataaag attcaacatt atatacgaat
     2041 tatgttaacg ccaatactat taattatttt gctgaagtca attataaaac agaatcttct
     2101 agtggagata cagaaaatac agtctatatg ctttttgaag taaccaataa agacggccaa
     2161 gtgttggtag ataattatga aatcaagtac ttaaaatagt gaggtgaagt atatgaataa
     2221 gaaatattcc attcataata agaaggaacc aaataaaaaa aataaggcac aaattgacaa
     2281 agtatataaa aaaacgaaag gagtaaaatt atatttaggt gctatacttg gatttttagc
     2341 aataggaatg atgatcgttt tttctcctta tgtgatgggg aaaagttatg attttgaaac
     2401 tgtaaaaatg agttcagccc aaaatgtaac gtcttctttg aatatgtatg ttgatgatat
     2461 ggaaataaat agggaagccg gactgtttaa attagtgttg aggtatcaag atgatacagg
     2521 tactaaatca ctaagtaata ttaaaagtga ctataaatta aattttataa cgaacaaaaa
     2581 taatgaagat gttaagacaa aagttgtaaa attgtcagat gaatacacgg taatttatta
     2641 tcaaaatcta cctaaagatt ttggtgtaat ttctgtgaca gttaacccta gatatattta
     2701 tcctgaatta gaaccttcaa atgacttaaa agaaaaggaa ataaaattct atgcagtaga
     2761 caaagatatt aaatcaaatg ctaaattgga agttgaaagt acttctgatt tgcaaaaaga
     2821 taattttaaa tatcagatta aagctataga aaattctata aaagatgaga aaaaatcaat
     2881 tgaaaaaatg aatttagcaa ataaagtagt gcaaaaagat attgataaag ctaatcaaaa
     2941 cttagaattt caaaccctag acgaacaaac agatacaaag aatgaaatca gttctatgca
     3001 gacgactatc gaaaataata aatcttcaat agaggaaagt gaaaataaaa tcaaacaatt
     3061 tgaggaaaaa ataaagcaac tagaaaagtc tgcaagccga tttgagtgat cgtgtttaaa
     3121 ttattttaag ggagtttaga gagaatgtgt aattctattg tcactattca agagaaagct
     3181 gaagaagaat taatgagaat tgaagcggaa tataaagaaa agagaaaagc aattaagcgc
     3241 aaaaaaaatg cagagcttaa aaaatataaa gaggaacaag cgttaaaatt aggaatgatg
     3301 ttgtgtaaat attatgattg ttatgatata aacgaagtgt taaaaaaagc gaatttaaac
     3361 caattttgtg aatagaaaag agagtggcaa ggtaaaactt gtcactcttt ttttgtgcgt
     3421 aaaagcttta gttactatga ccaaaactac ggacgaaact atgaccaaaa gtaggtacta
     3481 tattatgacc gaaaataggt cgaaaatgga tttatttgtg atagcaaaaa atatgaagtg
     3541 attttgaaag aaaaccagtt ggcacagcca acttagccac cttcctttag gaaggtgcta
     3601 tcagttttcc cttatgctct ttgcattaat tctttggttt cgtctttaaa tctttaattc
     3661 tcctttagac tttgatataa taatatatac cagatagata aaaactattt gaatgaggtg
     3721 tatatatgga cgtttcaatt agagggattg atccgcaata tgtaaaagag attgataaaa
     3781 aagttaaaaa aattcaagaa cgtaccggaa aaaagtttag taggaatgac tattttttag
     3841 caatcattcg ccaagatagt gagattgatt tgattgatta taaaaagtca gaatttgatt
     3901 tagtagttga aaaactatta ttgtcaaatg agcaaatggt agaaagtatt agcgagttaa
     3961 cttcagttta tgaacgctta ttcaattttt taattagcga aaggagtgat ctaaataaat
     4021 gaactaccct tatttcaaag tatcagcaac agaagatgta aaagaaaaat tcaataagtg
     4081 ctttgaagta aacaagtcag tatttcaaac aaaagctaat atgttccgat atgttgtttc
     4141 aaatttacca atgttaacaa aagccaatca aaatttccgt gatccagaaa aagaagagtt
     4201 agctaaaaac ttagaagaag caaaattaaa aattagcgaa cttgaaacga taatagaatt
     4261 gcaaaaacag caacttgaaa actttaataa aaaagaagac agtatacaca aagattatag
     4321 tgaacaatta aatagtatag aagagtctgt ctataaacta ttaattaata gtggattagc
     4381 tagtaaagaa ttagaagaag agcgtgctga accagaaaaa gaaagtagtc gtttaggaga
     4441 tggaacaatg tttagttcac cgtcattaag atttacaacg gattgaacac taaaaaattc
     4501 taatgcaact tagcattaag tacaaaaccg cctgtagaaa aaaattttga aaatggtggg
     4561 tgatgatatg actgaacata tctttaacag aaaaagtgcg agcattattc ttaaagcaaa
     4621 atttgaaact ggaaaaaaca aaaagaagaa tcacatggta gaacttcaaa aatttgtaga
     4681 ttacatttcc agacaagagg ctattaggca agataaatta gatcatgatt attccgaaga
     4741 tgaactaaat gaattagcta gaatagaaaa agctttggaa aaaatagaac aggaaacaga
     4801 tcaaccagtg attaaagatt tgcgtgaaat ggataaatat attgattata tgacacgtaa
     4861 aaaagctatt ttagaaaata tagataatga aatagtaaac ggagctttta gtaatacaaa
     4921 aagatatatc actaaaaaag acatttcaaa aataaaagaa tcggttatag aagcaaaaaa
     4981 taatggatcg gtgatgttcc aagatgtaat ttcattcgat aatgattttt tagtaaggga
     5041 aggatattat aatccagaaa caaatgaatt aaatgaaaat attctatatg aagcaacaaa
     5101 atctatgatg ggaaaaatgc aagaaaagga agaactagtt gatccttttt ggtttgcaac
     5161 gattcacaga aatactgaac atatccatat tcatgtaaca gctatggagc gtaaaaatac
     5221 tcgtgaaata atggaatatg atggtgtttt acaagctagg ggtaagagaa aacagtctac
     5281 gttagacgat atgattttta aattcggtag caaaatatta gatagaaaga acgagtttga
     5341 aaaaatttct aagctgagaa aagaagttcc tttagaactt aaacagtctg taaaagattc
     5401 tttgattcaa ttatatgtag aaaataattc aagatatgac aaagaattag taaagtacct
     5461 aaaggaatta aaaaaagaaa tacctagtac aacacgtggc tacaatgagc ttcctgaaga
     5521 aacaaaagaa aagattgatg aagtcacaaa ttatatgaca aaggataatc caaaaagaaa
     5581 agaatatgat cgtatgacta aagaaattga tgagctatat caaacaacct acggtaaacg
     5641 ctacaatcca caagaatatt atttaaatag aaaaaaagat tttaatgctc gaatgggtaa
     5701 tgctgtagta ggacaaatca aacttttaaa gcaatctgaa gaaaaaaatt tgaaaagtaa
     5761 tgaacttaaa aatctattag acggaaaaaa attaaacgtt aagtttgata gagaaaaacc
     5821 agaatttaaa acgaaatatg ctaaacaaag ttatgatgat ttccaaaaaa gattagaaga
     5881 acgacaatta gcacgtgaaa aaaatgaagt ccacttcaag aagatttctg aaaaatttaa
     5941 agataaaaag cttattagaa aaattgatag agtaatcaat gatgatttaa atatgtatcg
     6001 tgcgaaaaat gattatgaag tagcgcaacg aatgatagaa caagagaaaa tgagacaagc
     6061 acatgaattt gaaataggaa tgaattagta gaaacggaga atagaaatta tgaaaaactt
     6121 cgatatagtg tgcagtaata caaaaaatat atatttacgt gagctactta acagtgattc
     6181 ggaaactata gaagatgtaa aaaaaatcat agtgttgttt gaaaaagaaa acatggagct
     6241 agagaattgg gggctttttg aaataccgat tagtgggaat tactgttttt ataattggaa
     6301 gacagaagac gatgtagctt ttgcaaatta cttttttgat aaaaattact tttccccatt
     6361 atatatagat aagcatagta atgaacaagt tgcttcttca ataaaagaag caataaaatt
     6421 ggaaagagtg aggaaatgat atgatcgatt ttagaacggt tatggataaa ctaagtaatg
     6481 gagattttcg cgtattgatt gaactatact taaatgtaac gtctatagtc aaagagtttt
     6541 tatattctaa tcccaaaata gctttagtat tagttgcagt actgatagtt tttgctgtga
     6601 tctcttttat taagaaaatg attccgctat taattatagg tgctgtgttg atattaggtt
     6661 tgtatttccc tgaagttttg gatcaaataa aaaatatatt ttaggctcta aaagcttcca
     6721 gtagaaaatt ttttagatta cttgtactaa caacataagg taaaaaggag aaaattgatg
     6781 aaagtttcta ttgcaaaaga atcaattgat agaagagaag ctattattag tgaactaaaa
     6841 atcactgaaa aattgatgaa agaaaaatag cgaaggaggt ttaagatgta ttatcaactg
     6901 ttgtacgaca aaataataga tgcagaaacg ttttattata aagaaaaaaa cgaatggctt
     6961 aatggattaa gtttatatct aagatttgat ccaatctttt atgtagaaaa taaacagaaa
     7021 tcaaaagaac aaatgattca gctaaaaaac gagcgaagtt tattgaatcg cttagttcgt
     7081 gtctcttatg acaatcaaac aagaaaatta tcgtttcatg accacgagga gataaaagga
     7141 aaataccgag tggcaaaata ttataaacta gtcaccgatg aagagtattc aggttggtgt
     7201 gaagaagaaa aaattagtaa ggaagaattt tattttctta tccaacatta cggacaacat
     7261 tttcacaatg acttttctct gcaatcctgt tcatatggtt ggcatgaaat agaaagtctt
     7321 cctgaaggaa caattgttca aaatagttat ccaaaacatt taaatgaaac gacaaagcgt
     7381 ttggaagaaa tcgtatttga agagaaaaaa cggcatgaac agttgctaag tgttaaaaaa
     7441 gggataatga aatttgaata ccgatcagaa tgtttagaat ttattaaacg aatcgaactc
     7501 ttggaaaatc ataaagactt tctattgaat aaggaacaat tcgaccaaga agaagtgatg
     7561 gaatcttatg ttcaattgta tacggaatgg attcgtttta atcgatttat gtcacaagaa
     7621 aatctctaca cagcttaatt ttagagtttt aaagcttccg gtagaaaatt ttgggctttg
     7681 atatttgttt taagcggttt gaaatagaaa tttgataaac tacatgaaaa gacattatat
     7741 agtcgtgtag agcgaattag tgggtttaaa tatcgtttga ctacttgtag tcaagtgtgt
     7801 taagctattt ttgtagtaat aagacataag aagttaaagg aagttattgt acttccttta
     7861 atttaagcta aaaagactac aagtagtctt ttcggttttg agaggtgaaa atatataatt
     7921 aaacttggta ggtgaagaaa atggaagaca agaagtattc aaaaattata aagaaaataa
     7981 aagggctttt agcaatagcg aaagatgaaa atcaggatga agagagtcaa tctgctttcg
     8041 ttttagcaca aaggcttatg ttgcaatata aaatagataa aaatgatgta ttcgatactg
     8101 aagatgaaat agaaccaatc ggtgaagagt cagtaacgat ctataagaaa ctatattggt
     8161 gggaacgatc gctaggatca ataatagccg agaattttag ggttaaaatg ttttacaata
     8221 gtaaaaagga aaaaggagag aggaaaagta aaagtaaaat agtattttat ggattcggaa
     8281 gtgatttaga gttagctaag gaaatgtatt ttttagcgta tgaagttatt gtatttcata
     8341 ctaatcaata tataaaaaac tggtatcaag atactgatat gaaaagaagt agatatataa
     8401 cagaaagtgt gaaatcaagc tatatatccg gatttttagt aggtctgcaa aaacgattcg
     8461 aggaacaaat ttctgttttg gccgaacggt atgaagtaat ggttttgatt ccaaaagctg
     8521 tagaagaatc ctttgataaa tatagtaaag atttttctag ttatagtact aatactccac
     8581 cggtatctgt ggatcatgcg taccaagagg gagtaagaga agctaaaaaa atagatttaa
     8641 caaaaagcac tgttgataca gattatagta gtttagtagg tcgtattatt aggtttaatc
     8701 aaggagtaac tcgtggatta atagctgaaa tcactagtgt ttctgacgat ctattaaatt
     8761 tgttggtaat gaattgtgtt tcagaatatg cttctgaaga taacccttct ttctatcatt
     8821 ggaaagtaga taaggagtac gactatgaaa tcctttcttt caacgatccc atgagtaaaa
     8881 agtttaaaaa aaataaagta gacataatgg aaaactaaaa aaagtgataa acaaaggact
     8941 gattatttgg ttctttgttt tttgctatat ttttctacag gcaattatct taagagtatc
     9001 tgtgtattgg aataaaaata tgataatatg aaattatgta tcagaccgat aaaaaagagg
     9061 tgcttataaa tgaaaaagtt aagagttatt gcaacaatac ctatgatttt tagttttgcg
     9121 ctattattat ctgcatgtgg taaagaagaa gctggtgcta agtcaaatga agcacataat
     9181 atctcattca tagatagtgc agaacaaaaa gattcacgtt gttggttggt agtcgaaaca
     9241 agtagtaaaa ataaaacgcc tattgataag aacgaatatg taaaagcggt gatccaaact
     9301 aaagatggca aagcaaaagt atttaaaacg gatatggaac agcttacatt ggaaaaagtt
     9361 tctaaaatga gtgatgaaga aataatagaa caagcattga acgctgataa acaaaagttt
     9421 aatgataaga aaaaaagcga tattgatagc tacaatgcta ctttagattc tgtaaaaaca
     9481 gaattagaaa atgaggaaaa acaagagttt caaaatgacc aaaaaattgc tgattttcaa
     9541 gatagtatac ctaaaataca ggcggatata gaagagatcg atggtgttca atattcagaa
     9601 ccggagtggg aaaaggtatc agcaacaatt aaaatggata atgaaaacaa atcaccttca
     9661 atggaaaccg ttaaattatc ttataaatat ttagatagtc caatttggaa tttaaatagt
     9721 tttggtgaag tttcgtctga aggagaatta tcttatgaac ctactagtat ggatttgaag
     9781 ttgtcaattc cagttgccac aacgaaagtt ttagataatt tttattcggg atattctaat
     9841 gatgaattgg atcaactttt gcttactaaa gtagcttcta aagataacaa agttcaatta
     9901 gatagtaaag gtaaaaacgt aagtatagtt gagtagatgg taaaataaga agggataaga
     9961 tggttactat aactatctta tctctttttt atttgcatta agtcagttaa attagataga
    10021 agagttttta tatatctggt ataatttaat taggtaattt aacttaccta aaaaagaaag
    10081 gagatagaag atggcctata aaaaaggaaa ctggaaaccg aaaacgaaag agcaaaaaca
    10141 aaaagagcta gacgatttaa ttaaggcttc caacagcaaa atcgagcaat atcagtctaa
    10201 ccctgaagat atgatcgagt ttgccaagtt catgtctaaa atacacaatt acagtccttt
    10261 aaacctatcg ttgatcgatg agggatttga aggagcaatt gctgtagcaa gttatgatgg
    10321 ttggaaaaaa ctaggatttc aagttaaagc gaaagaaaaa gggataggca tttatgcgca
    10381 tgctccggta acggttttta ttgatgaaaa tggtgaggaa aagacgttaa aagaagcgac
    10441 aagcgagcaa aaagaaaaaa tcaaaaatgg agaattaaga agcaaaaaga tcaagcattt
    10501 taagaagggt tacgtatttg atatttccca aactaatgca actgaagctg atttgccaaa
    10561 aatatttcct aacagggtat ggaattttaa tattgatggt gaattaagtg ttagtcaatt
    10621 agaaaaaggt gtaaatcatt tggccgaatc tatgaatata tctataaaag atatgaatga
    10681 gtctggtaga ggtgaaatgg gaagcgctag aggtgaatat attcaatatg ttgatggtag
    10741 ggaagaaatt gtattaaatt caagaaatag cagaacacaa aatcttacta ccagtattca
    10801 cgaattggct cacaaaaagc ttcacaataa gaatgaacac ggttcttcct actcaatacc
    10861 gattaaagag tttcaagcgg aaatgacttc atttattgtt tgtcacaatt acggtattga
    10921 tacgtcagac tttactattc cttatattgc aaattggact aaaaataatg aaaaaattga
    10981 tccaaaaaac tttagacaga taatgcaaga agttagagaa acgtctattg attttataag
    11041 tacgattgat aagaaagtct tggaagaaca gcaaacagca tttaatgcac taaaaaattc
    11101 ctttgttcct aatgagaata aagagcagat acaacaaaaa tcagatttag aatttcataa
    11161 tttctacgat agtacaatta acgatccgga tagaacaatc cacggtagtt atttagaagc
    11221 gatggtggat tattatagag gaaagtatgg agaaaattct attgagctta ctcatagcaa
    11281 ttatgatgtt acagctaaaa taggagaaaa ctatgtgaca tattattcag atacgaaaca
    11341 atttgatgta tgtgatgttt atggtgtagt ttttgagaat ccaagtgaaa ataaaaatga
    11401 tcgtttatta ttagatcgat cacctgaaaa ttgtgattat gttgaaaagt ttgttgtaga
    11461 aagtttgaaa gtttgcgaga atcatgaact aaaaaatgat acgttaaaat taagcgaacc
    11521 gctaagatca gttgttataa atgaagcggt ttataatcta tctgaaaatg ctaacaatta
    11581 ctataatgaa aaatgttggc aagaagaaat tgaaaaccaa acaaatatta atgatgtaaa
    11641 agaattaaac tcttgtatga ctgcggaaga tttttacgaa tctcataaac aagaaatcga
    11701 aaatacgatt acagattttg aatctgtgtc aggaacaaat ttaagagata caatagacaa
    11761 tgagtcatat aaagaggtgg cttcagtggt agcttataaa ttggttgcaa ctaaagtaaa
    11821 atatgaaatg gatgaaggaa ctttcaagat accagaaaat tctaacttgg aaaagccgac
    11881 tgtaaaaaca gcagaacgtt cttttgaaat ggagtgaaaa aattatgagt aatgaacaaa
    11941 attatgaaac aggcggaact tatcgcctgt ttcatggaag tatgaataaa tttgatgaat
    12001 ttagtttaga tcataaaggt ttaaacggaa cagctaatgg atttggtgtg tacttaacac
    12061 ctaataaaga aatggctaca atgtacgctg aacagggtag taacggaaat aatggtttcg
    12121 tatatgaagt aaatgttgaa ttagagaagc cgatttctat gaatgaacgc actattactg
    12181 atgaagagct ttctaaagta atagataaat tacaagaaag taatgatatt ttaaatgatt
    12241 acaatgatgt tagttattac ggagaaaaag ttgtaaaaag agaagtgatt gcttctctta
    12301 gtgagaatga aaatgacgta gatttgatta acgacattgg aaatacaatt ggagatcatg
    12361 aagctactgc taaggcattt tatgacgttg ggaaatatac acatattatt gcggaagaac
    12421 agttaagaca aaaggatgta gttatcgtag tgttacagcc tgataaagta aagattgaaa
    12481 ctattcacga tttaaacgag gaacggaata gcttagaaag tagtgaggac atgacagata
    12541 aatttgttgg gcataaggtt tcggaagttc ccattcgaga agttgtgata aaagattttg
    12601 agcaagagct agaaacaact aattattatg acaaacaagc ttggcaagag cgcattgaat
    12661 acttagaaaa catggaagaa ccagtttcca gattagcatt agatagtgat attaatgaat
    12721 tttatgatag tcataaagaa gaaattgaaa cgaaattgac aaatttatcc gaaaagttag
    12781 agatcgataa agaagaactg ctaggttttc cagtagatac ttcttctacg gaatacaaaa
    12841 aagctacgtc tatcatggct tacaacggtg caatgagaga aatttataat gaaattgaaa
    12901 atgatacgtt tgaaatacca cctgtttctg aagaaatgga gaataagatt ttgaaattga
    12961 ctaattttga aaatacaaca aaatttgaaa tggagtagat aaatgaatga tagaacaaga
    13021 aaatagaaat tttttagaaa cgcaaggtga atatcgtcaa attaataata gtcattttgt
    13081 aatttgtgaa gatgtacaaa tcgcttttaa aatggctttg aagtacggaa aaagtattag
    13141 aaaaaagata attagagaaa aaaagagcaa gaagataggc ttttgggagt tatctcttag
    13201 ggatgggcta aactttattt tctgtacaga ggattattat gaaaaataca agtgtacctt
    13261 atcgtaattt tactagtgat ggtctagtaa agggggtttt tgtttggatt tgaaaaaggc
    13321 aaaaaaagta agtatcgttg atttttgcaa acaaaatggg ataggtttaa agcaagattc
    13381 aagggaatac tatagattag aagatcatga ttcttgtgta atcagtgatc gcaaaaacct
    13441 ttttaaatgg aactcaaagg atttaggggg agatattttt gattttatca aagcctacta
    13501 tgattgttca tttaaagaag caaaacagaa acttgaagat agcgattatg aagttcattc
    13561 gttcaataca aaagtgaaaa aagctccgta ccaatatcag aaagaaaatg aagtgccaat
    13621 atctgttgca aagtcttatt tagtaaatga gagaagaatc gatacagctt tagtggaaga
    13681 ttttttatct aaaggactaa ttaaacaaga taaaaaagga aatgttttgt tcttatggaa
    13741 aaaagatcaa gaaatcgttg ggtgtacgga acaaggaact agaaaatttt ttcatgaagg
    13801 caaacaaaag gatgttacat ggaaaaaaat acaagaaaac tctaaagagt attcaggatt
    13861 taacgtaact tttggtcaac cagataaaat gtattttttt gaaagtgaaa tagatttgat
    13921 gtcgtacatt acacaaaagc cagaaaaagc cgaaaatgct acgtttgtta gcatgaatgg
    13981 attaaaggta ggtaatgtat tgcatggggt tacagagcat tataataagt ttaaatcatt
    14041 gccttcagaa acagtccttt gtgttgataa tgataaagcc ggacgtactt ttattgaaac
    14101 tgaatttaaa gaaaaaggaa ttggaagaga agggatagga atcagtgaag taaaagtcga
    14161 tgtacctttg cgagagggat ttgattggaa tgataattta acaagaggaa aagaagcttt
    14221 taaacgcttc aagttagaag atgtatcaaa aatcgtgaac ttcacacata aattatctct
    14281 taatactcct gtatatgaaa tggaataaca agaagaaagt agttgatgtg ttatgaacga
    14341 tacaatagca atcttatcag attcattaat tgatttagat aagacgtcta cagactatat
    14401 gattattgaa gaacaactta aaaataaaaa tccagagttc gatattcaag aaaaatcatt
    14461 gcgtattgaa ttaccaaaat ttaaacaaaa aaaattaata actattctct ttgagattat
    14521 tgaacgatat gggcttactt attatcaaat ttggacggat aagaaagatg gccaatatac
    14581 aaaaggggta agcatagaag ccgttagaga agacgatcaa gtaagtatta aagcagttca
    14641 acccttaatg gggtataacg aagaattatt gaattgttta aacgtctagt gtaaactagg
    14701 cgttttttta cattatttgg ctacgaatag acagttaatt aaactaaaga aaaccattga
    14761 ctacaagtag tcattcctgt atgatatagc catagactac aagtagtcaa agactcaaac
    14821 ccttgattta ataaaaagga gagataatag atggagcaga caagaatgtt ttggtcagat
    14881 cgaatgcaag atcacttgga ttgtatttac aaaaccagag agcaattaaa taagttagat
    14941 actgtcagat attattttac ctacttcagt gagaatgacg aacaaagatg ggatattttt
    15001 catgatggta attacgaaga atttttttac actgaaagtt aattatttgt tgaaatgatg
    15061 aaggattcta tttgatgaaa gggaacggaa aaccgttccc ctaaaaaata tccaaatgac
    15121 tacaagtagt catggaagga gagcggaata atggtaaata aaaaagaaac gtttaaagta
    15181 ggtatggaag tacagggcgt tgttattggg agcggagagc ttatcgaggg agaagtaatt
    15241 agtttgttag ctaatgtagc ggtagttaaa gcaatcggaa aagcaccggt agtgatgaaa
    15301 aaatgcagca tgatgtgtct tgatggttta aacaatccaa taactcaacg ctttgagaac
    15361 gtgatataat agaaaaaaga agattttcta ctgggagaga ttattatgaa agactatgct
    15421 tcacgtaaat ttatccactt agagcctatg aaagaattgg ttattaatgg gaatgaatat
    15481 aatgattaca aatctttatt tgtaaaggaa cagcctaaag gcggtattcg gatgaaaaat
    15541 ttaccggact tccgatttat cgaagtaaac aatttaggaa ttatagaaga ctatttagat
    15601 aagttagatg aagcgataaa gcaatcaatt gaaaaaaatg gtgaagatca tatacttacg
    15661 gttgttttaa aagacttgaa agatagacta gaagctgaag cagagtactt taataaagac
    15721 taataagaag ggaatgagag ctatgacaga ggctgtaaaa gaattaaaga aaatgtatcc
    15781 tgatgttttg aatatgactg tagatgattt tcatgaagcc ttaaaaaatg cagaaagtga
    15841 ggaagaaagg actttctacc ttacactttc tagttttgtg acacgagtag atcaaaaaaa
    15901 agtaattaat cagaaagact ttaaaatatg agtttagaaa atgaaaaacc gtattatgta
    15961 gttgttgggg gcgttaacgg cgccggtaag tctactattt atcaagttca aaatttaaaa
    16021 agaaaagaac ttgatactga tagaattaat gcagatgaaa ttttgagaag ttttggcgga
    16081 aattggaaaa aacaagcaga cataaacaaa agtatgcgtg aagttctaag agagatcaaa
    16141 ggtcatttta aagaaggaaa atcttttcac catgaaacga cttttggtgg taatccatat
    16201 cccgataaaa tgagacttct aaaggctaaa aaattaggtt atgaatcgtt tttaatctat
    16261 ataggactag attcaccaga aagagcgatt gaaagggtta aaatccgtgt tgctaaaggt
    16321 ggtcacggtg tgcccgaaga tgtaataagg aaacgatacg aattttcttt aagaaactta
    16381 gcagagttag aaaaatattt tgataatgta agtatctatg ataattcaga tgagttttct
    16441 tatatttatg ttaaagaaaa tcatacagtt gtagccaata atacgcaaga atataattgg
    16501 gttcccaaag ctattcaaga gaaaaacgct gaaattccta taagcaaaca aattatcaag
    16561 gatatatccc aagaagggtt gagtccaaaa gaaacccttg aaagaatata tttagcaagt
    16621 gatgatttta ttagtgatcg tttttttaaa gcagagcaag ttgatgattt ttatatgaac
    16681 cataaaaatg aaatcaagga acggattcta gcaattagtt tttctacagg agtacctgaa
    16741 gatgaattag taggccaaga taaaaaaaga aattcttgta aattggcata tattaatagt
    16801 gtaggtgagc ttttaggaaa aactgatagc gagctatcag gcatggaagt aaacagagat
    16861 agaagtataa aaaaaactga ttttgaaatt gaataaaaga agatgtagct gaatcagtta
    16921 atagtaaata aatttaataa ggagtgcgaa agcacttctt attttttaaa tagaatgact
    16981 acatgtagcc agaagggata atatattatt tttagttctt gttttttaaa tgattactac
    17041 aagtagacaa ttgattgttg attatggtct aataaatatg ttagtattat tatataccag
    17101 atacataata atgaggtgct aaaattgaca ataataacta tgactaaaaa aatagaagta
    17161 tatgttgatg gatcacaaga ggaaagagat tctttttttg ggtttttttg gaaaaatagt
    17221 agagaatatg taaaagtgct gaatatggta tatagacagt tgatgtttga agagctgata
    17281 aaagatgcaa tcaaagaaat ggatcaaggc tatgttgaga gagaaaaaag tattcaaaat
    17341 aggattgaaa agttagcgta tactgatcca atagatcaaa aaaaacttga taaagcaaaa
    17401 gctgaattat ctaaattgag aaatgaaaaa aataaagaag cgagagaaat attaacgcaa
    17461 gcaataggat tgaagagtca aacgagaaca cgagatgtag caaaaaaatt agactttgat
    17521 tttggagatt ttgttgattc agcaaatatg aaagctagta aggattttgg caatgacttt
    17581 gttggaattg ttactggtca aagaacacct agagtctata gaaataaaag taaatatgga
    17641 ttgttaccgt tcagaggtcg ggatattaag attcataaag ctgacaagtt ttatatctct
    17701 ttacctaaag gatttaggtt taaagtaaat ttaggtagca aaccgagaaa agcgagagat
    17761 agcgctttaa ctttggggta tattatggac ggtaaataca agttaaatca aagtaaccta
    17821 atttttgaag ggaataaatt gttttttaat ctaacaattt cttttgaaaa agattatcat
    17881 aagctagaat ttaaagaagg ttctgagtta accataaagt ttaccgattt gtgtagcggt
    17941 gaatgtttag ttaataataa atctgtgatt gattttggtg acagcaaata tataagtaac
    18001 ttcaaaaata aaattaaagc aatgaaatca agagagcaga gccgaggagt tttttctaaa
    18061 ggcggtcatg gtcgaaaaag aaaattagat caagatagat gggaagccat aaagaaaaga
    18121 gaaagtaatt ttgttaagac ttataataat caatgttcag caaaaattgt tcaaacagct
    18181 attcgcaata aggcacaaat aatttgtatt tctgaaccag ataaaagtta tggggaatgg
    18241 gcttattatc agttgaaaga acaaataata aataaaggaa aaagatacgg aatagaagtt
    18301 aaaagtgtgt aaaataaaaa atcgttgatt tggcaacgaa atagtgaggg ggcactcact
    18361 cactgaaggg tcaactccgt catattgtgt gaccagacgg aaggacgaat gttgttgagg
    18421 ttttggagct atataaaacg acatggttct caaatgataa agccattctt tttcaattaa
    18481 tgattgtttt ggagctatat aaaacgacat ggttctcaaa tgatacgaaa gaagagtata
    18541 tccaaaaagt tgacaaggct ttggaatcat ataaaatagc atagtactca aaaatagata
    18601 tagtaatatt ttggaaatag ttatagataa gtagagaaat cgttgatttg gcaacgaaat
    18661 agtgagggaa actccactca ctgaagggtc aactccgtca tattgtgtga ccagacggaa
    18721 ggacgaatat tgttgaggtt ttagaaccat gtaaaacaac aaagttctca aatagaataa
    18781 gttcaaagaa ggtttagatg atggttttag aaccatgtaa aacaacaaag ttctcaaatg
    18841 tgtagataaa taatttttaa atattaatgg gttagcatat cttttaggtc ttagttacat
    18901 gctaagacta tttttttggt ttatatgtta cgtaagggta taccctattg taacactagt
    18961 ggaaatgcga tataatagga attagaggtg tgacacaagg gtgtgacata aaggggtgga
    19021 tatactgaag acagaaaaga aaaaattgta gcaaccaaaa aagaaaaaat gagagttgat
    19081 aaactcacga aaaaattcct agtttgtttg actagagtaa attaagttat ttgggtgtga
    19141 aaatatgtta gtaggatatg caagagtaag ttcagacgat caaaacctag aacgacaact
    19201 tgaagaattt aatcgtgtgg gtgtcgaaaa agtatttgct gaaaaaaaat caggtcggaa
    19261 tacagccgaa cggccagagt ttaataagat gttagcgttt gttagagaag gagatactct
    19321 tgtctttgaa tctcttgaac gattaggaag aaattctgat gagatattgg atactgtatc
    19381 gtttatagat aaaaatggta tgagtcttat ggttttgaat ttgccaattc tagatacaaa
    19441 gtttggtgat cctaacttag aaaagcttgt ccgaagtttg gttattagta ttttttcttg
    19501 gacggcacaa aatgaaagag aagaaataaa gagaaaacaa aaacaaggta tagcaattgc
    19561 gaaaaggaag ggatattaca aaggaaaacc ttatgagtat tcagaaaatg ccaaaaatcc
    19621 acaaaaaaga tttatttata acgagattgt tcgaatgtta ggtgaaggat taccaatcaa
    19681 aaaaattgca gaaaaaacag gaacaaatag aaatttagtt tatagaataa agaaaagaat
    19741 aagtgaatct ggttgatctt aagttctttg ttaaatcaac gttacgagag gaggattcta
    19801 agcctgtttc aaacttttaa gatgtacaac tatacaaaga agcttctaaa taaaagaggt
    19861 tatattgggg aatctctaat atagatcatt gactggaagg atagtttata tattcaaaga
    19921 agggtgttga aaaaaaaagg aagaaaaaac taataatttg gatttttaaa gataatacag
    19981 tcaaaaggag tttttaaaaa tgaatcaacc tagaagaaaa aggttagaaa gtatttctga
    20041 aaaaatttcc gagttgaaag atttgcttga ggaaattaaa gaggaagaag aagaatacaa
    20101 agagaatatg ccagaaaact tatgggggtc agaacgctat gaaagagccg aggaagccgt
    20161 agggcaatta gaagaagctg acgattattt ggatcaagta attggattaa tcgaggaagt
    20221 aatcagctaa ataccaagct agcaaaagaa atataatcaa aattaaataa aggggaaata
    20281 acaatgactc aaaactatat tgataactgg agattatttt ctggaaaatt aatgacaaaa
    20341 gacagtattg cagaaatgcc agaatcattg gaaaaggaac atattcttaa agtccgagaa
    20401 atgttgcaca aggaagggtt taatgcagat ttattccgag ttggggaact tccaattgaa
    20461 ggagtttatt attttgagcg agctgtatcg gagaacggag tcgattattt ttcaataatt
    20521 gttgaagacg atggttctct tacgccacag tatacaacgt tagaatgcga cgaaagcgga
    20581 tttaatactt ttccttgcaa taccattcaa gaatcaattg ataagcatga gtgctaatta
    20641 attatctaca ggaaggtgtt ttttatgaaa ttagagataa aagaagttgt ctgcgattgg
    20701 ggaatctatg tagacggaca aacatatcca tttatgattt ttaatagtaa agcaaatgca
    20761 caagagataa tgcgtatcat ggagttggat aataagcacg aaagatttga tgaagtaaac
    20821 aaaatggaga acgaatgtga agcagtggaa taccataaaa catgcgccaa taataagtat
    20881 attttaggtg caatggggtt aggtaaaaca gcttctatta aacaggtcta cgaagaatca
    20941 caggagaatt gcgtatatga aaattcgaat taaagagtga gaatatgtgg gagctaattt
    21001 atttagtttt atacttaata ggaaaatcaa ctgaatttgt atataacaat aaggtaatat
    21061 ttatattgtt cttaatagtt tgtattatcg tatttgat
//
LOCUS       DAXWOO010000036        21063 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.036, whole genome shotgun sequence.
ACCESSION   DAXWOO010000036 DAXWOO010000000
VERSION     DAXWOO010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.036"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            471..1673
                     /locus_tag="ACGE70_001048"
     CDS             471..1673
                     /locus_tag="ACGE70_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFF8692698.1"
                     /translation="MNLTRDLIFLVIYILGMSGILYWRKRQSDPALFFYCGTWTFMMM
                     VTYFVVLEVFYHTFYFLIPLLFFSIFAFFYFKEKRRLINGVLFNTFLIVFGGYLGWLL
                     IETQNIFIGGLLALIGIPILILLAIGIYILIIFLFWNGVVVMKKESRSLANLLTLLLG
                     VALTIFLILDYTLVHWLPQWANALFAVIPFIFAYLLIVFFNFMTVSILYQFNRPRYNQ
                     DYIVVLGAGLIDGERVSPLLAKRINKAIAFYWAQSKATLNPPVLLMSGGKGTDEKVAE
                     AIAMKQYALEKGIPERDILVETNSTTTLENMLFSKEIMDQQMNGPYRAIFTSNNYHIF
                     RAGLYARQAKLNANGIGAKTALYYLPNAILREFIAILVLHKKRHLIICGLMIGFAVFS
                     ALILKFAQ"
     gene            complement(1850..3118)
                     /locus_tag="ACGE70_001049"
     CDS             complement(1850..3118)
                     /locus_tag="ACGE70_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFF8692699.1"
                     /translation="MENRLPDPLKVIIYMIKWLLITAILGIFMGSLSAFFLTSLTDVT
                     AIRLEHPWLLFLLPISGGLFAYLYTTYGGNSSRGNNLVIDQGNGGNEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALAENFARWLKLNKIEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEALFPSFFAALFANFVTESFNVKHTHYQMGAIPAWDPS
                     LFFKLLLAGIAFGIIGWLFSRSIVWLKAWYAKRIPHPVLRNFIGGAVVVLVVVILQTQ
                     RYLGLSLPLLQDAFNGSAHPYDFLGKLGFTVLSLGAGYQGGEVTPLFEIGATLGATLA
                     PILHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGGQAASYFFMICLISFMCSGN
                     SGIYSAQKVEVKKGILFLPILGRISRQKEK"
     regulatory      complement(3169..3236)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(3279..3926)
                     /locus_tag="ACGE70_001050"
     CDS             complement(3279..3926)
                     /locus_tag="ACGE70_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HFF8692700.1"
                     /translation="MEKTIKPYQYYFIKREVEQLLSAYSSVNDPKTVQTVQALAAEKI
                     HEVLGHPNTAVNEFMEKVLDVTLKKEKAERLLEELKVIVLPFIQPTKPQIDKLFRKVK
                     KLKQPDWNQLDLREHTYIGWNDPGSQKKFMIYYEEEKLKGISGTLSPTINKGICSICQ
                     RTSNVSLFLSTTKTGSDGTYTKKGNYICHDSDQCNQQLVQLAPFYEFTERVKRER"
     gene            complement(4191..4694)
                     /locus_tag="ACGE70_001051"
     CDS             complement(4191..4694)
                     /locus_tag="ACGE70_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFF8692701.1"
                     /translation="MTEKWREDEEYMSYINDLLETDAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYKLAKKWGGDARATARAGLLHDLFYYDWRTTKFDEGTHAYVHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMTASMKQKWQRY
                     FGKEQSI"
     gene            complement(4818..5582)
                     /locus_tag="ACGE70_001052"
     CDS             complement(4818..5582)
                     /locus_tag="ACGE70_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFF8692702.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKGEYLLEGFHLIEEAVAS
                     NAKVNELFITQRGLTEWASWLEENQALSVTLVSEEVMATVSDLPTPQGIMAVVEAEKR
                     EIPNFEGGWLLLDNVQDPGNVGTMIRTADAFGLSCVVLGKGTADVYSTKVLRSMQGSN
                     YHLPIIQEDLTKLLPILKEQGFITYGTELNEEAVSLNDIQTTENYAIVMGNEGQGVRP
                     EVLALTDKNVYIPIQGNAESLNVGVAAGIMLYHFSV"
     gene            5690..5965
                     /locus_tag="ACGE70_001053"
     CDS             5690..5965
                     /locus_tag="ACGE70_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFF8692703.1"
                     /translation="MNKIKMNVQGRVQSVGFRYMTKIVADQLGITGTVKNEDDGSVTI
                     EAAGDDEAIKKFVEEVKRSPSPSGRVQYVDLQENPMMEDRKKFVVIG"
     gene            complement(6313..7464)
                     /locus_tag="ACGE70_001054"
     CDS             complement(6313..7464)
                     /locus_tag="ACGE70_001054"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFF8692704.1"
                     /translation="MDKSKIARRFQQPAENLLMNIAILAKEKEDIIDLSIGDPDLITD
                     PTIIEAAYTDVKNGATNYTAPDGSKEFLSTVVDFYQKKYGLSVECNQVRATVGAMHGM
                     YLVLQALLDPEDEVIIHEPYFSPYREQVILSGGKPVFVPTYEEKGFQLDVEDLKAAIT
                     DRTKVIVINSPNNPTGAVFSESTLKKIAEVAIENELYIISDEIYEDFAFKEKFVPMAR
                     FAPDNTITVSGVSKGFAMTGWRLGYMIAPAYINQIAGLINENITYSAPTASQRGAIYA
                     LKHYEQLVPPVVEVFKERLAYIEKRVAEIPYLSLSSVSGSMYAFINISKSGLDSVTFV
                     EEVLEKSGVLFVPGKAFGETIGDNYIRLAATQPLEILGEAFDRLAKLNF"
     gene            complement(7484..8206)
                     /locus_tag="ACGE70_001055"
     CDS             complement(7484..8206)
                     /locus_tag="ACGE70_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFF8692705.1"
                     /translation="MLNVKKLSKSFGENEVLKSIDLSVKEGEVVVIIGPSGSGKSTIL
                     RCMNLLEEPTSGEIFFKGQNITAPDSDINQIRQKMGMVFQSFNLFPHMNVLENLTITP
                     VKIKKEDPKKAKEHALSLLNQVGLKEKVDSYPTSLSGGQQQRVAIARALAMNPDVMLF
                     DEPTSALDPEMVGEVLAVMQDLAKKGMTMVVVTHEMGFAKEVADRVIFMADGIIQEEG
                     TPKEIFENPQNSRTQDFLNKVL"
     gene            complement(8199..9650)
                     /locus_tag="ACGE70_001056"
     CDS             complement(8199..9650)
                     /locus_tag="ACGE70_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFF8692706.1"
                     /translation="MKKGINILLVALMFLLVFLPVKVQGAEKDEIYERIKQSGELVVG
                     LSADYAPYEFHANVDGKDKIVGFDVSIAEKIAKDMGVKLKVEELGFDALLGALKTGKI
                     DLVISGMAVTEERLQEVDFSDPYFVVQQKVLVRKEDKNKFKTTADFSGLAVGAQKQTT
                     QEELVKNELTGAELTSLQKVPDLINNLLNEKVEGVVLEQPVAEAYAQQNKKVALTPVK
                     FAQGEKTTAIAMSKNTPELKNHVNASIKTINDDGLLKDYQKQANQLMFQDDQSFLQKY
                     GPYYLIGTKYTILLAFVGVLFGMVFGSLLALMKLGKNKFLKALAIIYIEYVRGTPLLV
                     QIFIVYFGTGVLGLDLSKLVAGCIAVSLNSAAYVAEIVRAGINAVNKGQMEAARSLGM
                     DQKEAMRFIIFPQAIKNILPALGNEFVTIIKESSVVSVIGVSELIFQTGVVQGASFKP
                     FLPYVLVSLIYFVLTFSISRILALVERRMGLNA"
     gene            9792..10874
                     /locus_tag="ACGE70_001057"
     CDS             9792..10874
                     /locus_tag="ACGE70_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFF8692707.1"
                     /translation="MTKNLIVRGAPQEYEIKENAWDSLPEHLYKRHIDNVLILHGKTS
                     WKAVKDVFPDLSITKIFEYYGGECTDPLADSFVSLCKKKDFNGIIAVGGGKVADLGKL
                     IAYKTNLPIIILPTLAATCAAYTPLSVVYNEEGVMLRYDMFPKANDLVLIDPKIILHS
                     PREMMIAGIGDTLAKWYESHAIISQLTIKPIEVQVAEFAALRCREILLEKSAEAMEAM
                     DQQKINQAFIDVVETNILLAGMVGGFGDDYGRTAGAHSIHDAMTVLPDSHQQLHGNKV
                     AYGILVQLMIENKLAEIEHLLPFYHHLNLPTSLADMGLHLTESDYQAVAERACLPDEM
                     IHFMKETITPALVVQKMKELEQVTQG"
     gene            11208..12146
                     /gene="yidC"
                     /locus_tag="ACGE70_001058"
     CDS             11208..12146
                     /gene="yidC"
                     /locus_tag="ACGE70_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFF8692708.1"
                     /translation="MNKWKNWLLGSGLVTIMLFLSGCVRMDSNGNPDTSGIIYRILVH
                     PMGQAITYLVENFNWSYGWAVILMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     ATQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESTFYGIDLGNPSLVLVAIAGIAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGIFSCLQTFITNVIMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTPAEPAKQTKKQVQAPKNNAKNGRNAGKQQKRK"
     gene            complement(12327..12731)
                     /locus_tag="ACGE70_001059"
     CDS             complement(12327..12731)
                     /locus_tag="ACGE70_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFF8692709.1"
                     /translation="MSRQLVLISHGKFCEELKNSTEMIMGPQENIYTIPLLPEDGADT
                     YKERFLSVTAPLKDYVVLCDLLGGTPANTVSKLIMEGETIELYAGMNLPMVIEFINSQ
                     MIGAEPDFIGSGRESIVSVNQVIAGMGDDEEE"
     gene            complement(12747..13556)
                     /locus_tag="ACGE70_001060"
     CDS             complement(12747..13556)
                     /locus_tag="ACGE70_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFF8692710.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDDTPELKEMMKTHTQFFNTSNFFNTIITGIDLAIEEKEGVSSKETVSGMKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPVGILIWILAQVAVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKLLPAGIVGGVYWMLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(13543..14433)
                     /locus_tag="ACGE70_001061"
     CDS             complement(13543..14433)
                     /locus_tag="ACGE70_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFF8692711.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLKAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDQHVETMNYKAIERNFLMGALPWALSRMVPVFLAL
                     AFGGGLVQNVVAVLNGDLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHISYLILGFT
                     ITALLATVFSNVQLLGTSVASVVKDFPGTFNSLPMLAVALIGLALATISYKNDQKNDQ
                     HPVVSQQSQSDEGEIEDDEI"
     gene            complement(14446..14925)
                     /locus_tag="ACGE70_001062"
     CDS             complement(14446..14925)
                     /locus_tag="ACGE70_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFF8692712.1"
                     /translation="MAIIGVRIDGRLIHGQVANLWTTKLNIGRIMVVDDEVATNAIEK
                     SGLKLATPAGVKLSILPIEKAAANILAGKYDSQRVLIVAKKPDRILALVEKGVPIKEL
                     NVGNMSQTDETRSITKSINVVDADVEVFKKLDQLGVHLIAQMVPNDKAEDFMSLLIK"
     gene            complement(14942..16708)
                     /locus_tag="ACGE70_001063"
     CDS             complement(14942..16708)
                     /locus_tag="ACGE70_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase family protein"
                     /protein_id="HFF8692713.1"
                     /translation="MTRFEIKEEFLLDGQPFKILSGAIHYFRVHPTNWERSLHNLKAL
                     GFNTVETYVPWNLHEKEEGTYDFSKILDLSHFLELAQSLGLYAIVRPAPYICAEWEFG
                     GLPAWLLTKECRIRSADPRFMKYVKRYYQTLLPKIVPHLVTHGGNVLMVQVENEYGSY
                     GEEKAYLRQVKKYLEEGGIDVPLFTSDGPWQATLRAGILIDDDVFATGNFGSKAKENF
                     ADMAQFFEAHGKKWPLMCMEFWDGWFNRWKEPIIKRDPEELADAVKEALQIGSINLYM
                     FHGGTNFGFMNGCSARGAIDLPQVTSYDYDAPLDEQGNPTEKYFALKRMMAENFPEMQ
                     QMEPWIKETIVKKGINLKEKVSFFATLDTISKPIHSTYPQTMEELGQNTGYLLYRTTI
                     KKDAVNEKLRIIDGRDRVHVFLDEEKQAIQYQTEIGETIPLTLEKEDHQIDLLFENMG
                     RVNYGHKLLADTQRKGIRTGVMSDLHFITEWTQYCLPLETTEHIDFSKKWEAGQPAFY
                     RFEAELSEIGDTYLDLSEFGKGIVWVNGTNIGRFWEVGPILSLFVPEGLFRKGQNEIV
                     IFETEGRFSEVIDFVKEPIEKS"
     gene            complement(16754..17695)
                     /locus_tag="ACGE70_001064"
     CDS             complement(16754..17695)
                     /locus_tag="ACGE70_001064"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HFF8692714.1"
                     /translation="MITTVTMNPSIDIAYTLDELRIDQVNRVKTVNKTAGGKGLNVAR
                     VLHLLADDVLATGLVGGQHGAYIKEQLDQSGITHQFLPIKGETRDSIAILHGVNQTEL
                     LESGPVVTEEEQHKFTEHFARLMETTDIFTISGSLPQGFSKSYYAELLALTNKKGTRV
                     LLDTSGQTLLEVLEGKEKPFLIKPNLEELGELVHQTISSATSLDEIQSLLDHPLFEGI
                     NWIVLSLGAKGAIAKVGRHFYQVTIPKIAVVNPVGSGDSTIAGLAHALSKSYTIEETL
                     AFAMACGMANAQQEKTGWIDPVKVDYFRGEINVRKIR"
     gene            complement(17692..18687)
                     /gene="lacD"
                     /locus_tag="ACGE70_001065"
     CDS             complement(17692..18687)
                     /gene="lacD"
                     /locus_tag="ACGE70_001065"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFF8692715.1"
                     /translation="MNEEKIAAMKSLSTKEGIISALAIDQRGALKKMIKALGKEPTAQ
                     QITEFKELVSAELTPYASAILLDPEYGLPAAKARHQEAGLLLAYEKTGYDATTPGRLP
                     DLLADWSTLRLKEQTADAIKFLLYYDVDEDPEINHLKHVFIERLGSECAGEKRPFYLE
                     LVSYDAKTNDSTSLEYAKIKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVEGYSDG
                     EIAYSKKEAAAFFKEQSDATPLPFIFLSAGVSTALFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGREGAIEWLRTQGRQNIESLNKVINENATPWFDKVKEDSVTAK"
     gene            complement(18680..19873)
                     /locus_tag="ACGE70_001066"
     CDS             complement(18680..19873)
                     /locus_tag="ACGE70_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFF8692716.1"
                     /translation="MFELSKDKLKELGAEITTREIKQQPQLWKETAEILAQNQEKLAL
                     FFDQIKKAANGKRVRVIFTGAGTSQYVGDTAVPYLNEYGDNDHFIFHSFGTTDIVSSP
                     KEFLFPDEPTLLVSFARSGNSPESVATVKIANEQIKTLFHLTITCAKEGQLAKNAATD
                     ERNYLLLMPEKSNDAGFAMTGSFTCMLLSALWVFDQTAVEKKESYLQQLIRTGEDLIS
                     RDQEIQKLLPKSFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVIGFVNNQPYTRNYDLDILEEIKDDQITTSVIAIAQPGNIDFTGTSLLLE
                     ASISLPDAYLALPMIMTAQIIALDTSIKVANKPDTPSPTGTVNRVVKGVIIHEYVNKG
                     ETKDE"
     gene            complement(19891..20619)
                     /locus_tag="ACGE70_001067"
     CDS             complement(19891..20619)
                     /locus_tag="ACGE70_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFF8692717.1"
                     /translation="MESSSKGKSLYYQLVDVLKERIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELETQGYIYRRHGKGTYVSDLSDKSSDLAGAYSFTEEMRELGRVPNTV
                     ILEFKQMEANKKVAKNLNINLGETVFKLKRLRLADDEPLMVERSYLPAKLFIGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDADPLRIADGAPVLYLSRTTYNVKN
                     EIIEYTDSVARADQFHYKIRHVRK"
BASE COUNT         6113 a         4518 c         3670 g         6762 t
ORIGIN      
        1 gtgacagtga atgtatagaa aagcgagtgg ataaaaactg atttttcatt ctggcctact
       61 tgttccacgt tttactatgt atacttcttg tctatcttgt tttttccatc aaatcatccg
      121 gttcattcct tccacagatt ttgttgccgt taacatttta ttatgcaact ggtttattgc
      181 ttgaccgata ttttccaata aaaaaaaata tcgttttcgc attcattttc aatgttacag
      241 ttatttgtat ctacaaaagt cttcatcatg atcgaggtct aaaaaatctg ttaatttgaa
      301 gatttatgtt ttcgataaaa gcttttttca acataattcg ttataataga aacgtcgtga
      361 aaaaactgat tgactattta gtaactaact tttctatatt cgatatattt actactaaat
      421 gatgctatct tttttaagaa aaaatttaca aatcgaaagg agttcattca atgaatctta
      481 ctagagacct gatattttta gttatctata ttttaggaat gtcaggcatc ctctattggc
      541 gcaagcgcca aagcgatccc gctctattct tttattgtgg gacgtggact tttatgatga
      601 tggtcactta cttcgtcgtt ttagaagttt tttatcatac attttatttt ttgattccat
      661 tgctattttt cagtattttt gcatttttct attttaagga aaaacgtagg ttaatcaatg
      721 gcgtactttt taatacgttt ttgattgtct ttggtggtta tctagggtgg ctactaatag
      781 agacgcaaaa tatctttatc ggtgggttat tggctttgat cgggatacca atattaattc
      841 ttttggcaat cggtatctac attttgatca tttttttatt ttggaatggt gttgtagtca
      901 tgaaaaaaga atcccgttct ttggccaacc ttttgactct cttgttagga gtcgctttga
      961 ctatcttttt gatattggac tatacattag tccattggct cccacaatgg gcaaatgctc
     1021 tatttgctgt cattccgttt atttttgctt atctactcat cgttttcttt aattttatga
     1081 ctgtttcaat tttgtatcag ttcaaccgtc caaggtataa ccaagattat atcgttgttc
     1141 tcggcgctgg cttgattgat ggtgaacgcg tatctcccct acttgctaaa aggatcaata
     1201 aggcgatcgc attttattgg gcacaaagta aagcaacttt gaatccgcca gtcttgctca
     1261 tgtcaggagg aaaaggaacc gatgaaaaag tagctgaagc cattgcaatg aaacaatatg
     1321 ctttagaaaa agggattccc gagcgtgata ttttagtaga aacgaattcc acaacgacgc
     1381 tagaaaatat gctgttttca aaagaaataa tggatcagca aatgaatggg ccttatcgcg
     1441 cgatttttac ttcaaataat taccatatct ttagagctgg actatatgcg cgacaagcga
     1501 aactcaatgc aaacggaatc ggtgcaaaaa ctgcactata ctacttaccg aatgctatct
     1561 taagagaatt tattgccatc cttgtcttac acaaaaaacg ccatcttatc atctgtggct
     1621 taatgattgg ctttgcagta ttttctgctc ttattctcaa atttgctcaa taaaaaagag
     1681 cgtgggacaa aactaaaaat cagttttgcc acagactcta actccgaata aacggtggga
     1741 gcagaagcaa ctccttcgga aataagcgaa attcacaaaa atttaaaaag caattttcgt
     1801 gaatttcttc ttatttctcg gagttaaaca cttctgtccc aacctctttt tatttctctt
     1861 tttgtcgtga gattcttcca aggatcggta ggaacaagat tccttttttc acttcaactt
     1921 tttgcgcaga atagattcca ctatttcctg aacacataaa gctaatcaaa cagatcataa
     1981 agaagtagct ggctgcttga cctccaaaaa gttcgatccc cataacaaag caagcaatcg
     2041 gtgtattcgt tgctccagag aacaccccaa taaaccctaa tcctgctaaa aaaggtactg
     2101 atacatgaag aatgggtgct aatgtcgcac ctaaagttgc cccaatctca aacaaagggg
     2161 tcacttcgcc tccttggtat cccgcgccaa gtgatagaac ggtaaagcca agtttcccaa
     2221 gaaaatcata aggatgagca cttccattaa atgcatcttg taataaaggt aagcttaatc
     2281 cgagatatct ttgcgtctgt aaaatcacta ctacgagtac cacgactgct cctccaataa
     2341 agttgcgtaa aactggatgg gggatccgct ttgcatacca agcttttaac cagacgatcg
     2401 aacggctaaa gagccagcca atgataccaa atgcaatgcc cgcaagcaac aatttgaaga
     2461 aaagtgaagg gtcccatgca ggaatcgccc ccatttgata atgggtatgc ttgacattga
     2521 aactttccgt tacaaagttc gcaaaaagtg ctgcaaaaaa gcttggaaac agtgcttcgg
     2581 ttctcacttt tccgattgcc aatacttcta atccaaacaa tgtcccagcc aagggtgtcc
     2641 caaatactga gctaaaacct gcgctgatcc cactgatgac aagaatctca cgctcgattt
     2701 tgttcaattt tagccaacga gcaaaattct cagcaagtgc cccgcccatt tggaccgctg
     2761 taccctcccg accaacagaa ccaccaaaaa gatgcgtggt aatggtgcca aataaggtca
     2821 gtgggatcag ccgtaatgga atcttttcat ttcctccatt cccttgatcg atcactaaat
     2881 tgtttccccg ggaagaattg cccccatacg tcgtatatag ataagcaaaa agtcccccac
     2941 ttatcggcaa aagaaaaagt aaccaaggat gctccagccg aatagctgtc acatcagtca
     3001 aactagttag aaagaatgcg gataaacttc ccataaaaat acctaaaatt gctgtgatca
     3061 ataaccattt gatcatatag atgatcacct tcaatggatc aggtaatcga ttttccatct
     3121 catttcctcc tcaaaaaaac gcctactaaa aaaaggggta gccccgtttc tttagtaggc
     3181 gtcattagtt atcttacgat caacgtttaa aggcgaacac catcacctat tcacttcacc
     3241 aaataagata gcatattttt cagaaaaata caatgaaatc accgctcacg tttcactcgt
     3301 tctgtgaatt cgtaaaaagg agctaattgt accaactgct gattacattg gtcactatca
     3361 tgacaaatat aatttccttt tttcgtgtat gttccatctg aaccagtttt tgtggtagaa
     3421 agaaagagtg aaacatttga agttctttga caaatcgaac aaattccttt gttgattgta
     3481 ggagataatg ttccactgat cccttttagt ttttcttctt cataatagat cataaatttt
     3541 ttttggctac ctggatcatt ccaaccaata taagtatgtt cccttaaatc cagttgattc
     3601 caatctggtt gttttaactt tttcactttt cggaataatt tatcgatctg cggttttgtc
     3661 ggttgtatga atggaagaac gatcactttc aattcctcta atagtcgttc tgctttttct
     3721 ttttttagag tgacgtctag taccttttcc ataaattcat ttacagctgt attaggatga
     3781 ccaagtacct cgtggatctt ttccgcagct aatgcttgga ctgtttggac tgtctttgga
     3841 tcattcactg aactataggc gcttaacagc tgctcgactt ctcgcttgat aaaataatat
     3901 tgatatggtt tgattgtttt ttccatgact ttgagtcctt tcttctttca aaatcggcta
     3961 ctttgaagaa aaaagtgatc tttctatcaa agtgccttta gataaagccc tcaaactctt
     4021 ttaaataaga tacacatcgc atcttatcat cacccccttt atttttatgt tggttatgta
     4081 ctttttcatc tcaacaaagt aaacactaaa aagaccaatt agtcaatgtc taaacaaaaa
     4141 acccgaggat ttttttcaaa atactcgggt caaatactta gttaactacc ttaaatcgat
     4201 tgttctttac cgaaatatcg ttgccatttt tgtttcattg aagctgtcat tggttgagct
     4261 gcttctttga ctgcacaata tttatccaca aaagtaacga tataaccttc tttatattta
     4321 ggaggggcaa tcgttgctcc ccacatatgt ttcaaaataa tatcacgctc tagatctgaa
     4381 agctccgtga ttttttcagc atttttcaca gcgatccttg ggtgaacata cgcatgtgta
     4441 ccttcatcga acttcgtcgt acgccaatcg tagtaaaata aatcatgtaa aaggcctgct
     4501 cgagcagtcg ctcttgcatc accgccccat tttttcgcta atttgtagct atagtaagac
     4561 acactgattg aatgctctaa tcgtgtcgag tggacatgtt gcgtgtaatt tgctaatttc
     4621 tttactgcat cagtttctaa tagatcattt atataagaca tatattcttc gtcttcacgc
     4681 catttttctg tcataattat cgatctgcca cctttttgaa ttccatttat ctttgaaatt
     4741 gaacttagta taccactagt gttcgtaata ttctagtgag gttttcgcaa aatgcagaaa
     4801 gcttaatagt tccttaacta tacggagaag tgataaagca tgatgcccgc tgcaactcct
     4861 acatttaaag actccgcatt tccttgaatc ggtatataca catttttatc cgttagcgcc
     4921 agcacttctg gacgtacgcc ttgaccttca ttccccatca cgatcgcata attttctgtt
     4981 gtctggatat cattcaggga cactgcttct tcattcagtt ctgttccata ggttataaat
     5041 ccttgctctt taaggatagg caatagtttt gttagatcct cttggatgat cggtaaatga
     5101 tagttactcc cctgcatgct tcttaatact ttggtactgt agacatccgc cgttccctta
     5161 cctagtacaa cacaagatag accaaaagca tcggctgtgc ggatcattgt ccctacattg
     5221 ccgggatctt gtacgttgtc gagcaacaac caaccacctt caaaattcgg tatctctctt
     5281 ttttcagctt ccaccactgc catgatccct tgcggagttg gtagatctga aacagttgcc
     5341 atcacttctt ctgatactag tgtaacagaa agcgcctgat tttcttcaag ccaactagcc
     5401 cactcagtca gaccgcgttg cgtaataaaa agttcgttga ccttcgcatt tgaggcaaca
     5461 gcctcctcga tcaggtgaaa cccttcaagc agatattccc ctttttcttc acgatatttt
     5521 ttcttatgta atttttttaa ttctttgatt cttggatttt ttgctgattg gatctctttc
     5581 acatttttca cctcaactga ttataccatg gattagatca ttgcacttgt tcagtagcaa
     5641 aaaagtcggt atcattaatt taaaaataaa agaaagaacg gtgaataaaa tgaacaaaat
     5701 caagatgaac gtgcaaggac gtgtacaaag cgttggtttt cgctatatga caaaaatcgt
     5761 agcggatcaa ttagggatca ccggaacagt gaaaaatgaa gatgacggct ctgtcacgat
     5821 cgaagctgcg ggcgacgatg aggcaatcaa aaaattcgtg gaagaagtca aacgatcacc
     5881 aagccccagc ggacgtgtcc aatatgtaga ccttcaggaa aatccgatga tggaggacag
     5941 aaaaaagttt gttgtaatag gctgaccttc tagaaatgaa taggaaatgt cagaaaaatc
     6001 ggactgtctg tatgtagatc gaaaaagttt gcccctctct ccatcctaga tcgacaaaaa
     6061 aacattctta ttagcgagtt tagtaaccag tgataggcat acgtaaaagc gggaggactt
     6121 caaaaatttt ggttcaataa aatggagtct ataatatttt atggtagtgg aaaatcgact
     6181 ttgattttcc actaccactt tttcattgtc aaatcctgac gctgcccagt caacaagcca
     6241 actctcgaaa ggatcatttt ccctctttgt tctaatggat acccatagct gttttcaaat
     6301 atacagtttt atctaaaaat ttagttttgc taaacgatca aaggcttcac ctaagatttc
     6361 caatggctga gtcgctgcta gtcgaatata gttgtccccg atcgtttccc caaacgcttt
     6421 tccagggaca aaaagtacgc cgcttttttc taaaacctct tccacaaacg ttactgaatc
     6481 gagcccactc ttactgatat taataaaagc atacatgctt ccagaaacag aagataaaga
     6541 taagtaaggg atttctgcca ctcttttctc aatataagct aatcgttctt tgaatacttc
     6601 aacaactggc ggtacaagct gttcatagtg ctttaaggcg tagattgcac ctctttgaga
     6661 agcagtcggt gcggaataag tgatgttttc attgatcaac ccagcaattt gattgatgta
     6721 tgctggtgcg atcatgtacc ctaagcgcca gccagtcatc gcaaacccct tcgatacacc
     6781 actgacagtg atcgtattat cgggcgcaaa tcgcgccatc ggtacgaact tctctttaaa
     6841 ggcaaagtct tcataaatct catcggaaat gatatagagt tcattttcga ttgcgacttc
     6901 agcgattttt ttcaacgtgc tttcagaaaa aacagctccc gtaggattat tcggcgaatt
     6961 gatgacgatc acctttgtac gatccgtaat cgctgctttt aaatcttcca catccaactg
     7021 gaagcccttt tcttcatagg taggaacaaa aacaggcttg cctccagata aaatgacttg
     7081 ttcccgatat ggtgaaaaat aaggttcatg gatgatgacc tcatcctctg gatctagtaa
     7141 tgcctgtaaa actagataca tcccgtgcat tgctccgact gtagctcgaa cttggttgca
     7201 ttcaacgctc aatccatatt tcttttgata aaaatctacg accgttgaca agaattcttt
     7261 gctgccatca ggcgcagtat aattagtagc accatttttt acatctgtat aagctgcttc
     7321 aataatcgtc ggatcagtga tcaagtcagg atcaccaatc gataaatcga taatatcctc
     7381 tttttccttt gccaaaatag caatattcat caacagattt tctgctggtt gctgaaaccg
     7441 gcgtgcaatc ttacttttgt ccatcttact tcctccatct ttgttacaac actttgttca
     7501 agaaatcctg tgttcgacta ttttgcggat tttcaaaaat ctctttcggt gttccctctt
     7561 cttgaatgat gccgtcagcc ataaaaatca cacgatcggc aacttcttta gcaaaaccca
     7621 tttcatgagt gaccacgacc atcgtcatcc cttttttagc taaatcctgc atgactgcca
     7681 acacttcacc gaccatttca ggatcaagtg cagatgtagg ttcatcgaaa agcatcacat
     7741 caggattcat tgctaatgct cgagcaattg ctacccgttg ttgctgtccc ccagataaac
     7801 tggtaggata actgtccact ttttccttta gaccaacttg gttcaataat gatagagcat
     7861 gttcttttgc ttttttaggg tcttcttttt tgatttttac aggtgtgatg gtcaaatttt
     7921 cgagtacatt catatgggga aacaaattaa aactttgaaa taccattccc attttttgac
     7981 gaatttgatt gatatcgctg tcaggggctg taatgttttg tcccttaaaa aagatttcac
     8041 cagaagttgg ttcttccagc agattcatac aacgtagaat tgtacttttc ccacttcctg
     8101 aagggccaat gatcaccact acttctcctt ctttcacaga aagatcaatt gattttaaga
     8161 cttcattctc gccaaaactt tttgacagtt tttttacatt aagcattcag acccattctc
     8221 ctttcaacta aagctaatat acgggaaata ctgaaggtca acacgaaata aatcaaagag
     8281 accaacacat atggtaggaa tggtttgaag ctagcacctt gtaccacgcc cgtttgaaag
     8341 atcaattccg ataccccaat gaccgaaaca actgaggact ctttgatgat cgtgacaaac
     8401 tcatttccta gagctggaag aatatttttg attgcttgtg ggaagatgat gaaacgcatg
     8461 gcttctttct ggtccattcc taaagaacgt gcagcttcca tctgtccttt gttgaccgca
     8521 ttgatcccgg cacggacaat ttcggctaca taagcagcac tattcaacga gacagcaata
     8581 caacctgcta ccagtttcga tagatccaat cccagtactc ctgttccgaa gtaaacgata
     8641 aagatttgaa ctaagagagg tgttccccgt acgtattcga tataaataat ggctaatgcc
     8701 tttaaaaatt tattcttacc caatttcatc agtgcaagca aactaccaaa gaccattcca
     8761 aacaatacac ccacaaaagc taataaaata gtgtattttg ttccaatcaa ataatacgga
     8821 ccgtattttt gaagaaagga ctgatcatct tggaacatca attgattcgc ttgtttttga
     8881 taatctttca gcaagccgtc gtcattgatc gttttgattg acgcattgac atgatttttc
     8941 agttctggtg tattttttga catcgcaata gctgtggttt tttcgccttg cgcaaatttt
     9001 actggagtca aagctacttt cttattctgt tgggcatatg cttctgcaac tggttgttcc
     9061 agcacaacac cttcaacttt ttcattcagc aaattattga tcagatcagg aactttttgc
     9121 agggaggtca actctgcacc agtcagttca tttttcacta actcctcttg tgtggtttgc
     9181 ttttgcgccc caacggctaa gccagaaaag tcggctgtcg tcttaaattt atttttgtcc
     9241 tctttacgaa cgagtacttt ttgctgtacc acgaaatatg ggtcagaaaa gtcaacttct
     9301 tgtaaacgtt cttcagtcac cgccatacca gatataacta aatcgatctt gccagttttc
     9361 aacgcaccta ataatgcatc aaaaccaagt tcttctactt ttagtttcac acccatatcc
     9421 tttgcgatct tttcggcaat ggatacatca aaaccaacga ttttatcctt accatccaca
     9481 ttcgcatgga attcataggg cgcatagtct gcagataggc caacgactaa ttctccactt
     9541 tgttttatcc gctcgtaaat ttcatctttt tctgcacctt gtacttttac tggtaaaaaa
     9601 accagtagaa acatcagcgc cactaataat atgtttattc cttttttcat ccatttggcc
     9661 acctttgaat aataatgtaa tcagaaaagc tataaaagaa taaatattca taaataactt
     9721 gctaatatac tagcataact attataataa aaaaagatta atttaaaaaa ataatggaag
     9781 aagtttttta tatgacaaaa aatttaattg ttcgcggtgc ccctcaagag tatgaaataa
     9841 aagaaaacgc atgggattca ctccctgaac acctttacaa aaggcatatt gacaacgttt
     9901 tgattctcca tggaaaaaca tcttggaaag cagtaaaaga tgtttttcca gatttatcaa
     9961 taacaaaaat ttttgaatat tatggtggag aatgtaccga tccacttgct gacagttttg
    10021 tttctttatg caaaaagaag gatttcaatg gtattattgc agtcggcggc gggaaagtag
    10081 cagatctagg aaaattgatt gcctataaga caaacttacc aatcatcatc ttacctacct
    10141 tagctgcgac gtgcgcagct tatactccac ttagtgtggt ctataatgaa gaaggagtga
    10201 tgcttcgcta tgatatgttc cctaaagcaa atgatttagt gttgatcgat ccaaagatca
    10261 ttttgcactc ccctcgcgag atgatgattg ccggtattgg tgacacctta gcaaaatggt
    10321 atgaatctca cgcaatcatc tctcagttaa ctatcaagcc tatcgaagtc caagtagcgg
    10381 aatttgcagc gttacgatgc cgcgaaatcc ttttagagaa aagtgcagag gcaatggaag
    10441 cgatggacca gcaaaaaatc aatcaagctt ttatcgatgt cgtggaaaca aacattttat
    10501 tggctggtat ggttggtggg tttggcgatg actacggccg aacagctgga gctcattcca
    10561 ttcacgatgc gatgaccgta ctccctgata gccatcagca acttcatgga aacaaagttg
    10621 catacggtat ccttgtccag ctgatgatcg aaaataaatt agcagaaatc gaacacctac
    10681 tgccgtttta tcatcacttg aacttaccaa cgagtttagc tgatatgggt ttacatttaa
    10741 cagaaagtga ctatcaagcc gtagctgaac gtgcatgttt acctgatgaa atgatccact
    10801 tcatgaaaga aacgatcaca ccagcactgg ttgtccagaa aatgaaagag ttagaacaag
    10861 tgacacaggg atagttagcc ccttatacca tggcagtcag cttttttatg gatcggctca
    10921 atggtaaaaa aaaagaagtg gaaaaaattg acattccact tctttttttt gcgctaaaat
    10981 tatgatttca aggaaatatc cgttgctcat cttattttag agaacggagg tgctaagtat
    11041 gctttatact gaagtatttt tagctggatt cttcttttta acattaagcg tcttttttga
    11101 tcctccaatc ggttatttct atgaaatttc tatgaagttg attgaattat gagcgctttt
    11161 ttagtatagt taagcttgtg taaaaattat aaaagaggaa gtattttatg aataaatgga
    11221 aaaattggtt attgggctct gggcttgtca cgatcatgct tttcttgtcc ggttgtgtcc
    11281 gcatggattc aaatggtaac cctgacactt caggaatcat ttaccgtata ttggttcacc
    11341 caatgggaca agcgattaca tatttagtgg aaaactttaa ttggtcctac ggctgggctg
    11401 ttatcttgat gacagtcatc gttcgtatca tcattttgcc attaggtatt agtcaatcga
    11461 aaaaaacaat gatccaatct gaaaagatgc aagcattgaa accacaagtt gaagctacgc
    11521 aacaaaaatt gaaagctgcg acaactcgag aagaacaaat ggctgcacaa gctgaaatgc
    11581 aacaagttta tcgtgaaaat ggcttaagta tgactggtgg tatcggttgt ttaccgcttc
    11641 tgatccaaat gccgatcttt tctgcgctat atttcactgc tcgctataca gaaggcatcc
    11701 gtgaatcaac cttttacggg atcgacctag gtaaccctag cttagtttta gtagcgattg
    11761 ccggtattgc ttatttactt caaggctata tctcaactat cggtatccct gaagaacaaa
    11821 agaaaacgat gcgtacgatg ttgatcgtat cacccgctat gatcgtcttc atgtcgatca
    11881 gtgccccagc tggtgtcact ctttactggg tagtcggtgg gatctttagt tgtctacaaa
    11941 cattcattac gaatgtcatc atgaaaccaa gattaaaagc gcaagttgcg gaagaattaa
    12001 aacaaaatcc gccaaaacaa gtggtgacgc ctcgtaaaga tgtcacacct gctgaacctg
    12061 cgaagcaaac aaaaaaacaa gtacaagcac caaaaaacaa tgcgaagaat ggtcggaatg
    12121 ctggtaaaca gcaaaagcgt aaatagttat ttgtaactag cttcttcgat cgacccagat
    12181 catttctgat tttatgacga taaaagccaa cgtactgtga aatatagaaa actatatttc
    12241 gcggtacgtt ggctttttta tacacggcct ctttatacat ccgtatttat atccagttgt
    12301 atcaccagtc aagaagttgt gcttgattat tcttcttcat cgtctcccat gccagcaatc
    12361 acttggttca ctgaaacgat actctcacga cccgaaccaa taaaatctgg ttctgctcca
    12421 atcatttgcg aattgatgaa ttcgatgacc atcggcaaat tcatgccagc atatagttcg
    12481 atcgtttcac cttccatgat tagcttggaa acggtatttg caggtgtccc acccaacaga
    12541 tcgcaaagca ctacgtaatc tttcaatggt gctgtaacgg agaggaatct ttctttatac
    12601 gtgtccgcgc catcttcagg caataaaggg atcgtataga tattttcttg tggtcccatg
    12661 atcatttctg tactgttttt tagttcttca caaaatttac catgactaat caagactagt
    12721 tgtctactca tctttccaat tctcccttat ttcgaaatca cacctaatgc agataatgct
    12781 acacatacga tcaatacgat gaatattgct cgagtaggtg tcatattttt cttacctaac
    12841 atccaataaa cgccaccaac gattccagct ggtaaaagtt taggtaagat catatcgata
    12901 tagttttgca tattcaacgt tacatcccca atcgttggtg cccatgagaa tttaacattg
    12961 atcatggttg ctactaacgc tccaaccatg aaaactccta gtaaagtagc cgcatctgtc
    13021 aatgcagtca agcgtgattg catcgtggta actagagaaa tcccttcttt gtaggcaaat
    13081 tctagttgtt tccaacgaaa gaccatgaca gcgacctgag caaggatcca gataaggata
    13141 cctactggat taccatctgt tgccatattt gcggctaaag ctccaaatat ggtcgggatc
    13201 aatgccgcaa atacagaatc accaattgca gcgaaggaac ccatcaatcc tgctttcatc
    13261 ccagaaacag tttcctttga agagactcct tctttttctt cgatagcaag atcgatccca
    13321 gtaatgatcg tgttaaagaa attagacgta ttaaaaaatt gcgtatgtgt tttcatcatt
    13381 tcttttaatt ctggtgtatc atcaccatag atttttctaa gttgtggcaa gatagtatac
    13441 agataacctg aaccttgcat tctttcatag ttccaaccta attggaaagt aaacaaactt
    13501 ctacgattga tctgcttaaa atcttctttg gtcaatttat aattagattt cgtcatcttc
    13561 gatttccccc tcatcagatt gtgattgctg acttactact ggatgttggt cattcttttg
    13621 atcattctta taactgatcg ttgccagtgc tagaccaatc aatgccacag caagcatcgg
    13681 taatgaattg aatgttcctg ggaaatcttt gaccacgcta gccacacttg ttcctaacaa
    13741 ttggacattc gagaacactg ttgctaataa cgctgtgatc gtaaatccta aaatcagata
    13801 agaaatgtgt ttctttactg gtaggtagcg tagtaaaatt gcaaacccaa ctgctggtaa
    13861 gactgcccct gcaacagata agccatctcc tagccatttt aggtcgccgt ttaaaacagc
    13921 cacaacattt tggactaagc caccaccgaa tgctagggcc aagaatacag gaaccattct
    13981 ggaaagtgcc caaggtaatg cccccatcaa gaagttccgt tcaattgctt tgtaattcat
    14041 tgtttccaca tgttgatcga tccgatgtgc aaaatacgta ttcgcaaaac gtgccaaaat
    14101 atctaattgg atcatcaaac ttgctacagg aaccgcgatg gccgcaatcg cttgttctgg
    14161 gttcatccct agagaaatgg agaaagctgt tgctaagatc gttccagagt tggcatcgat
    14221 ttttgatgca ccaccaaatg tccctacccc taaaacagtc aactgcatac ttccaccaat
    14281 gattaaacct gcttttaaat cacccattac taacccagca tataatcctg caaaaactgg
    14341 ggcgctcatt gaagaataaa tctgtaattc atctaaaatt tgataccctg catataatgt
    14401 taataataat atctgccacc attgaatatc catgaatcat tcctcctatt tgattaaaag
    14461 actcataaag tcttctgctt tgtcatttgg caccatttga gcgatcaaat gaacgccaag
    14521 ctgatctaat ttcttgaaga cctctacatc cgcatctacg acattgattg attttgtgat
    14581 ggaacgtgtt tcatctgttt gtgacatatt gccgacattt aattctttga tcggtacacc
    14641 tttttctact agcgctaaga tacgatctgg ttttttcgct acgatcagca ctcgttgaga
    14701 atcatattta cctgctaaaa tatttgcggc tgctttttca ataggtaaaa tacttaattt
    14761 gactccagct ggcgttgcca actttaaccc acttttttcg atcgcatttg ttgctacctc
    14821 atcgtctacg accatgatcc gaccaatatt caactttgtc gtccaaagat ttgccacttg
    14881 tccatgtatc aaacgtccat caattcttac tccaataatt gccatcatcc attctccttt
    14941 tctaactttt ttctattggt tctttgacaa aatctataac ttcactgaat ctaccttctg
    15001 tttcaaagat aacgatttca ttttgtcctt ttctaaataa accctcggga acaaacagtg
    15061 aaagaattgg tcctacttcc caaaaacgac caatattcgt gccattcacc caaacgatcc
    15121 ctttcccaaa ctcactaagg tccaaataag tatcaccgat ttctgacagt tccgcttcaa
    15181 agcggtaaaa tgctggttga cctgcttccc atttttttga aaaatcgatg tgttctgtag
    15241 tctctaaagg taaacaatac tgtgtccatt ctgtgataaa atgaagatcg ctcatcactc
    15301 ctgtacgtat tccttttctt tgcgtatcag ctagcagctt gtgtccatag ttgacccgtc
    15361 ccatattttc aaataataga tcaatttgat ggtcttcttt ctcaagtgtc aatggaatgg
    15421 tctcaccgat ctctgtttgg tattgaatcg cttgcttttc ttcatcgaga aaaacatgga
    15481 cccgatcacg tccgtcgatg atccgtaatt tttcattgac cgcatctttt ttgatcgttg
    15541 ttcgatacaa taggtaccca gtattctgac ccaattcttc cattgtttga ggataggtcg
    15601 agtggatcgg cttgctgatg gtatccagtg ttgcgaaaaa gctgactttt tctttcaagt
    15661 tgattccttt tttgacgatt gtttctttta tccatggttc catttgctgc atttctggaa
    15721 aattttctgc catcattcgt ttcagagcaa aatatttttc cgttggattt ccctgctcat
    15781 ccaatggtgc atcgtaatca taggaagtga cttgcggcaa atcaatcgcg cctctggctg
    15841 aacagccatt cataaaccca aaattggttc cgccatggaa catatagaga ttgatactgc
    15901 cgatctgtaa tgcttctttg actgcatccg ctaattcttc cggatctctt ttaatgatcg
    15961 gctctttcca acgattaaac cagccatccc aaaattccat acacatcaac ggccattttt
    16021 taccatgtgc ttcaaaaaac tgggccatat ctgcaaaatt ttcttttgct tttgagccaa
    16081 aattccctgt cgcaaaaaca tcatcgtcaa tcaaaatgcc tgctcttaat gtggcttgcc
    16141 aaggtccatc agaagtgaat agcggtacat cgattccacc ttcctccaag tattttttta
    16201 cttggcgcag gtatgctttc tcttcaccat acgaaccgta ctcattttct acctgcacca
    16261 ttaagacgtt gccaccatga gtgactagat gaggaacgat tttaggtagt agcgtttggt
    16321 aataccgttt gacatacttc atgaagcgag gatccgctga acgaatacgg cactcctttg
    16381 tcaatagcca agcaggcaag ccaccaaatt cccattctgc acaaatataa ggggctggac
    16441 gtacgattgc gtataatcca agggattgcg ctaactctag aaaatgggaa agatccaata
    16501 ttttcgaaaa atcataggtt ccttcttctt tttcatggag attccaaggc acataggttt
    16561 ccacagtatt aaatccaagc gctttcaaat tgtgcaatga ccgctcccaa ttcgtcggat
    16621 gcactcggaa ataatggatg gcaccagata aaattttgaa aggttgaccg tctaacaaaa
    16681 actcttcttt tatttcaaat ctagtcaagc aaatttcctc ctcgttttta gaaaccgctt
    16741 tctaatttta taatcaacga atctttctga cgttgatttc ccccctaaaa taatcgactt
    16801 tcacaggatc gatccatcct gttttttctt gttgtgcatt cgccatccca caggccatcg
    16861 caaacgcaag tgtttcttca atcgtatagc tctttgatag agcatgtgct aagcctgcaa
    16921 tagtcgaatc tcctgacccc acaggattca ccaccgcgat tttaggaatc gtaacctgat
    16981 agaagtgtct ccctacttta gcgattgctc cttttgcgcc tagcgaaaga acgatccaat
    17041 tgattccttc aaaaagcgga tgatccaata atgattggat ttcatctaac gaagtagccg
    17101 aagaaattgt ttggtggacc aactctccta attcttccaa attaggtttg atcaaaaatg
    17161 gtttttcctt gccttccaag acttcaagca aggtttgacc agaagtatct aacaatactc
    17221 gtgttccttt tttattcgtt agggcaagta actctgcata ataactttta gaaaatcctt
    17281 gaggcagact acccgaaatc gtaaagatat cagttgtttc cattagtcgt gcaaaatgct
    17341 cggtaaattt atgttgctct tcttccgtca caacaggacc gctttccaat agttctgttt
    17401 gattcactcc atgtaaaata gcaatcgaat ctctcgtttc ccctttgata ggtaaaaatt
    17461 gatgcgttat ccctgattga tcgagttgtt ctttgatata tgctccatgc tgcccgccga
    17521 ctaatccagt agctaaaaca tcatctgcta ataaatgaag gacacgagca acattcaagc
    17581 cttttcctcc agccgttttg ttgaccgttt tgacccgatt gacttggtcg atccgtaatt
    17641 catccaacgt gtaagcgatg tcaattgaag gattcatggt aactgtcgtg atcattttgc
    17701 tgtcaccgaa tcttctttta ctttgtcaaa ccaaggggtg gcgttttcat ttatcacttt
    17761 attcaacgat tcgatatttt gtcgcccttg tgttctcaac cactcaatcg caccttctct
    17821 accattttca acatatgctt tgactccatc tttccaagtc gcacgtccgc ataacacacc
    17881 gttgaatgtt gaaccagatt catgagcaaa gatcaatgtt tgttggaaca atgcagtcga
    17941 aacccctgca ctcaagaaga taaatggtaa cggtgttgca tcactttgtt ccttgaagaa
    18001 tgccgctgct tcttttttac tatacgcaat ttcaccatca gaataacctt ctacatagtt
    18061 catattgact ggcacttcta ctttcaatac atccacttta tatttaggat cagaaaattc
    18121 tttcatcatc tcgatcactt tatgcggttt gatcttcgca tattctaatg aagtgctgtc
    18181 attggttttg gcatcatacg aaaccaattc tagataaaac ggacgctttt cacctgcaca
    18241 ttcactgcct aaacgttcga taaatacatg tttcaaatga ttgatctctg ggtcttcatc
    18301 tacatcataa taaagcaaaa acttgatcgc atcggctgtt tgttccttta atctaagcgt
    18361 agaccaatcc gctaataaat caggcagacg tcctggagtt gtcgcatcat aacccgtttt
    18421 ttcataggct aaaagtagtc ctgcttcctg atgtctggct ttagcagctg gcaatccata
    18481 ctctggatct agtaaaatag cagaagcata aggcgttaat tctgctgata ctaattcttt
    18541 gaattcagtg atctgttgcg ccgttggttc ttttcctaac gctttgatca tctttttcaa
    18601 agcaccgcgt tgatcaattg ccaatgcact gatgattcct tcttttgttg ataaactctt
    18661 catagctgca attttttctt cattcatcct tcgtttcccc tttgttcaca tattcatgga
    18721 taatgacacc tttgaccact cgattgactg tacctgtagg cgatggtgta tctggtttat
    18781 tcgctacttt tatcgacgta tcgagcgcaa tgatttgggc tgtcatgatc ataggtaagg
    18841 caagatacgc atcaggtagg ctgatcgaag cttctaacaa aagacttgta cctgtgaaat
    18901 cgatgtttcc tggttgggcg atcgcgatga cactcgtggt aatttggtca tcttttattt
    18961 cttctagaat atctaagtca taatttcttg tatacggttg gttattaaca aaaccaataa
    19021 caagtgtttc gctatttaaa aacgattttg gtccatggcg gaagcccata gaagaatcaa
    19081 agatcgttgc cactttaccg gctgtcaatt ccaatatttt caattgtgct tctctcgttg
    19141 caccagccaa acttcctgaa ccaagatagg cgatacgttc aaatgactta ggcaataatt
    19201 tttggatttc ttgatctcgt gaaattagat cttctccagt tctgatcaat tgttgaagat
    19261 aactttcttt tttctcaacg gcagtttggt cgaataccca taacgcactc aacaacatgc
    19321 acgtaaaact tcctgtcatg gcaaatcctg catcatttga tttttctggc atcagtaaaa
    19381 gataatttcg ttcatctgtc gctgcattct ttgctaactg accttcttta gcacaagtaa
    19441 tcgtcaaatg aaacaatgtc ttgatttgtt cattggcaat cttgactgtt gccacacttt
    19501 ctggactatt tccactacgt gcaaatgaca ccagtaatgt cggttcatcc ggaaatagaa
    19561 attcttttgg tgaagacacg atatctgtcg taccaaagct atgaaaaata aagtgatcgt
    19621 tatcaccgta ttcatttaaa taaggtactg ctgtatcccc gacatattgt gatgtgcctg
    19681 caccagtaaa aatcactcga actcttttgc catttgctgc tttttttatc tggtcaaaaa
    19741 acaatgctaa tttttcttga ttctgtgcta atatctcagc tgtttctttc cataattgcg
    19801 gttgctgttt gatttctcgc gtagttattt ccgcacccaa ctcttttaat ttatccttcg
    19861 atagttcaaa catgttgttt tccctctcaa tcattttcgg acgtggcgga tcttataatg
    19921 aaactggtct gctctcgcta cactatccgt atattcaata atttcatttt tcacattata
    19981 ggtcgttcgg cttagatata ataccggggc accatcagcg atacgaagcg gatcagcatc
    20041 tttcttttga acgatacttg cataaaattc ttcatctgct aattggactt gctgttcata
    20101 atcctgagaa aacacatcat acaatggctt actttcaagt aatggcagat caagaccaat
    20161 aaatagtttt gcaggtaaat aagaacgttc caccatcaat ggttcgtcat ccgctaaacg
    20221 aagtcttttc aatttgaaaa ctgtttcgcc taaattgatg tttagattct tggcaacctt
    20281 tttgtttgct tccatttgct tgaattccaa gatgactgta ttaggcaccc gtcccaattc
    20341 cctcatctct tctgtaaaac tatacgctcc cgccagatca gacgacttat ctgatagatc
    20401 agacacatat gttccttttc cgtgtcggcg ataaatatac ccttgcgtct ctaattcttg
    20461 caaagcaagg cgaaccgttg tccgactcac tccataatgc tgacaaagtt ctctttcaga
    20521 taacaatttg tcatgaggtg tcatctgtgt ttcgatacgt tctttcaata cgtcaactag
    20581 ttgataatat aatgattttc ctttacttga gctctccata aaaatacctc ttctttactg
    20641 gttataacca caacacaaga atacccacat attcctcatt tgtcaaaggg tttttggcgt
    20701 aaaaaagcta ttataacaag gtttcaaggt aatttagaac tggtatcaaa agatacctca
    20761 aaaataccag ttgacaaata aaaaacaaaa aaaagctgaa tcttggcaaa gattcagcta
    20821 aatggtctag agaagtaaag aaaaaatatt ctttatacat ctgtggctat ttctactatc
    20881 gacaagcaaa tgggcctaat agactactat gtaaataaaa atggctttct ctaaataaag
    20941 taggattttt tattttttag ataacttttc tctcatgccg gtctgatagt cgaaattttg
    21001 ttgtacattt ttaaaaataa caaaaatttt ttatgataat tctgtctttt tctagtttcc
    21061 tta
//
LOCUS       DAXWOO010000037        20566 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.037, whole genome shotgun sequence.
ACCESSION   DAXWOO010000037 DAXWOO010000000
VERSION     DAXWOO010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20566
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.037"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(<1..1435)
                     /locus_tag="ACGE70_001068"
     CDS             complement(<1..1435)
                     /locus_tag="ACGE70_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692718.1"
                     /translation="MFILDDEIKKVKNPRIIPLLEEVRSSFYQGNYRSAIAVNYSALI
                     LDLIDKLSDLANIYEDNTSKKILEDIARLRKSDPNSPKWEMELLEKAYSSTELIDSYE
                     YEDLKYIKDQRNYSAHPVVTYNGNTWELRDITKETCQDVIRKSYEIIFLKNPILGKKV
                     TIEIIQYASRIHAMSVTPEEFHSALKNSYLNKLSEKAKENLCKTAFKFVFQLSYGNEE
                     ADRDRESTFRLLNALIFDNKEFYLNILKKEIAKYRFTAESREEINKSLGENQQITYTK
                     GFFLLTFIATHPELQHDFSDETIQNLQISINDFKPKQQVLTEIEYYYRLRSLAVLGNN
                     NEELQNHLNSLVDPILQQKMRVSSLPSSTLETIYNQYLYFGEKEMFIDFMIEHLTDAS
                     SFRLADKDMEVLPWIYNKLNQEQFNKLLYNLNGNNQFYKNSNFPPFISNLLDFYNQKF
                     QVNLRKHYSGLLYPNAVTMDQGFQLKDK"
     gene            1557..2141
                     /locus_tag="ACGE70_001069"
     CDS             1557..2141
                     /locus_tag="ACGE70_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692719.1"
                     /translation="MDLKQSFYIWLYPDNPMSIMADIFVVSILLYLFYLIFFHNLIGY
                     MILDWKGMFDIERKPFANNQTYTVFESGDWEENEVDVIHEIGLALSGIQDADVLVSMT
                     TDTGYNAYEVFYLMYFIWRDGQEIINGRAEKTINSNTEISFCMGEGEMIVYKDGSANI
                     KTAFGDIETSIKELREYVQNPYNFQYLAGRINLV"
     gene            2152..2397
                     /locus_tag="ACGE70_001070"
     CDS             2152..2397
                     /locus_tag="ACGE70_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692720.1"
                     /translation="MKKIINQPTNHLIIEGVIFVTMYFTVGNGISNTIIQKNPQYLLF
                     SLYAWIVQMVWVSILMLLFYWIVRVILLLLNKRKKDR"
     gene            2403..3125
                     /locus_tag="ACGE70_001071"
     CDS             2403..3125
                     /locus_tag="ACGE70_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692721.1"
                     /translation="MKSTKLKVVLLSTAALITLTACGTTNSANNNKDTKSSSTMVTKN
                     STTSASTTMSSMTENNAEKYQKYEDINKGQKPDEVYNNFINTVKNDENYGNDPLQNKN
                     LTIVLYKPDCPDCQKAESKIMQEIKEGFSYAPTLMHDITINKPAYEYYAVAVDITDGV
                     PEWVQNIPGLADENNQYHTPTVAVVTPKKVNGTLYWDVMGMTTNTTDEMIESTTKYYM
                     DLGAYKLYQQSEPVMDDQSKVK"
     gene            3256..3999
                     /locus_tag="ACGE70_001072"
     CDS             3256..3999
                     /locus_tag="ACGE70_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692722.1"
                     /translation="MKEISANEAHIPQKNQVGIYKVLHGEGIYKIQLLTGYNNGANGK
                     WLKATSIRELRSEVEKIRLGLTSEEIAKLKGNNSFVIEKEKEKHLLYRELKIEEYYEI
                     DLPEKLRIIDQRSEEKWKTEIYPSLNDYKTSGKPMYNYTGFLTYTQYKYAFRKLKGIK
                     QAEIALYEMKKVDRLKLIQDLVQNTWNQAIVELEKENGKILTNKYLIVNEIEELIGLF
                     SCEKGEVFLKSSPTYSILDSPYQFIQARR"
     gene            4005..4370
                     /locus_tag="ACGE70_001073"
     CDS             4005..4370
                     /locus_tag="ACGE70_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692723.1"
                     /translation="MSSLKDDYIQAKQEYEKIQMLKSEKVELFLTQKLAEDHIANLDA
                     KTLEEYDRQFNQIHHNIILLVEQAKRRFEKAEYDLLDQGIVCLDKGLRRYYGAELKKQ
                     NVDRNALKHEILELIMQRI"
     gene            4386..4916
                     /locus_tag="ACGE70_001074"
     CDS             4386..4916
                     /locus_tag="ACGE70_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692724.1"
                     /translation="MSQNISAVKQLIEEDLETTVQRLAERIHFANYQTISNWSSRNRN
                     PEEIPMKFFYRIGKEYGVDPIKIFEKTMIYYYHGTPNEVLEHLLEEAKEKSSILELSQ
                     NDKVIQVKCVDPTGKIIVGFDIERKSKIDFITQLKKINNLDILTLRPDHEQLFMSFKK
                     QLKEIQKRYNKVFPNA"
     gene            5132..5386
                     /locus_tag="ACGE70_001075"
     CDS             5132..5386
                     /locus_tag="ACGE70_001075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692725.1"
                     /translation="MMIGMRIQREQSYFEVSANNVIERCHYGQHFWTGEVRENIDREY
                     VQRIVWDAYSIKDYPKSLTSRLFYSEKDNQKLSDLMMKKV"
     gene            5493..5801
                     /locus_tag="ACGE70_001076"
     CDS             5493..5801
                     /locus_tag="ACGE70_001076"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692726.1"
                     /translation="MYESEFINLTFLNSVFIDYVITTKRIGSYSSTYASLVPMLNSMS
                     RFLKEREKKELQYIKEIMPDFEMHEWQIALSDWKLANKVRRITPFQAKRFLRAMKFVQ
                     "
     gene            5938..6738
                     /locus_tag="ACGE70_001077"
     CDS             5938..6738
                     /locus_tag="ACGE70_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692727.1"
                     /translation="MSEDLRELKLRKKQYDQYFSGLLNKHIEEGLDHDFGKDEYCLYC
                     ERYNEKMSEYLQLKKEIQKLEKKVHAWKYKDSMSGYVESQNKPRKKKQAYVLKMVNGE
                     ELIVLAYSEEEVRELVETPVRKKVETINEIDDSQYNSIYIETSHGGYKKLATFLRSSK
                     VGIISKKDISAVTDSFCTLSVPKAYFQYDSKRFERLEKFCRPYTVLKTKKFNKTKRVS
                     IQLPTGQISLGEKDYLIKVGKELIIKVPEHLFKENFEIRETLVWKEEK"
     gene            6775..6957
                     /locus_tag="ACGE70_001078"
     CDS             6775..6957
                     /locus_tag="ACGE70_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692728.1"
                     /translation="MAVNKILQVLIIDEFGGGEWFDVQSIDFEEEVVVIENIYGEVYS
                     YSLEGENIEDLRLIGE"
     gene            7068..7385
                     /locus_tag="ACGE70_001079"
     CDS             7068..7385
                     /locus_tag="ACGE70_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692729.1"
                     /translation="MKREEYKQRLNELLEEDETLTHGSPDEILYMIDNMVIFGGYELG
                     NRSVDHNILEFDDVSWEEILDWGILAVPETKTYISDTMVPFFEELDYKRLPKNENHIL
                     GGN"
     gene            7388..7531
                     /locus_tag="ACGE70_001080"
     CDS             7388..7531
                     /locus_tag="ACGE70_001080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692730.1"
                     /translation="MLSDLFILLIYLGVSEIIAYFLFVIANYGISSAIVQMFRLPVSE
                     LKK"
     gene            complement(7735..8214)
                     /locus_tag="ACGE70_001081"
     CDS             complement(7735..8214)
                     /locus_tag="ACGE70_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692731.1"
                     /translation="MTDKTSDLPKLAPKTSRVKSKKDGKTKNAVKKKSVKETSSLLLD
                     IPMSSFDDNTDETSVKIATHVGMNNQNDSFYNLLNSLRELEDKKNQVIASKAPDLTTD
                     LDAEIIQIKQDERSVKSLLKTYFDGFDKELAPYSQAILTKPIQAPHISISLQKKSDD"
     gene            8298..9671
                     /locus_tag="ACGE70_001082"
     CDS             8298..9671
                     /locus_tag="ACGE70_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFF8692732.1"
                     /translation="MKKSKRKIATLLMLMVQFTGIAMSVITINPQVIHADGIATYDAQ
                     FNCTTEGSGSGTDSDGNDTGSGAVTGDVAKNIEDIYKYMNGEYGLSGASIAGILGNWK
                     QESGIDPRAVEGYYGDGQREVDFIKQFTDGLMGIGYGQWTAGRHTALVDWSKKNNGGS
                     WWTTEAQLDFMFKGDGGFVGILKDYALNASDDVVENAVTFHKNWEISADSESAVIAVR
                     GENAKEIWKYMKSKGMDGAKDESKINKISGGSSTGDGSNNSSSSASGTVEDACATTDD
                     ANSGSGSIGKEGKINGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFNGIYQ
                     GQCTELTWAYMSQLYDGNQPTTGNGGYVWESYKSAGAKITNKPIVGYGFSASDGWLWA
                     VGDAGHTGVVIAVFEDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFSGVGK
                     AKFEIEK"
     gene            9694..10221
                     /locus_tag="ACGE70_001083"
     CDS             9694..10221
                     /locus_tag="ACGE70_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692733.1"
                     /translation="MNSKVKDTIIISLLSGMLLALLVGILFFNKQNQDIEAEKHSVHI
                     EDKLTFFKEVKPNEVDGLRKDLENDIQEMLNGSVQNKFADQSGEEFLRKLFIVTGKPP
                     ITDKSSEKEKIEYYKPFTFKLTNFGIKTSSSGLYEALTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            10208..10771
                     /locus_tag="ACGE70_001084"
     CDS             10208..10771
                     /locus_tag="ACGE70_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692734.1"
                     /translation="MQNHEITTAKKAIGILSIIVILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRKFFNLFYNWNSWEKYTKNMKEIQ
                     KEFPQIVKSSPVDISGTMVGTGNSPISSYTADYYTTNQKGKMVELITQTRNTPTTATT
                     TIWRGVTSIGENQLFYLEDLDSYVRAE"
     gene            11193..11930
                     /locus_tag="ACGE70_001085"
     CDS             11193..11930
                     /locus_tag="ACGE70_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692735.1"
                     /translation="MADHKLEKLLEETWNPKEFSEFFMENFETDLAVIVKDALREQGY
                     PETANYININFTLYTENKGTWDFWATLANKELSDKSDTGIRNFFESNRDDYMYANNQN
                     KLNFRVEFDETPEEFIERQPPKENVAKVLEDRWNSDEIVSTISELGGQYEPLVEAVRE
                     ELRLNKFPDVQNIDVSQIEINVKITNKLDYGSWADIALEKYIYSTLKEFIENRMDIMY
                     LQHPQYLNFGVEIATPLEEWKMEQGLD"
     gene            complement(11947..12363)
                     /locus_tag="ACGE70_001086"
     CDS             complement(11947..12363)
                     /locus_tag="ACGE70_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692736.1"
                     /translation="MNKKRKLSVLITLVSILTILSVILSLKIFNQINTSSNAKDGIVV
                     ENTSNSKETGTTTKKYKSAEITTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDK
                     FNGTFSLQTAEGSPDNLKVGQTIILGKNDELYSFVK"
     gene            12543..12758
                     /locus_tag="ACGE70_001087"
     CDS             12543..12758
                     /locus_tag="ACGE70_001087"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692737.1"
                     /translation="MNLDKYNLYTAREASLILGKGRNYVTNIINYYPNRLPKGSFKKV
                     GDKTYIITEEGIEALRSQTENKNKKKE"
     gene            12755..14002
                     /locus_tag="ACGE70_001088"
     CDS             12755..14002
                     /locus_tag="ACGE70_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFF8692738.1"
                     /translation="MNMKRNSLGMVLLASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNISK
                     DTLTVKEADGEVVSQTVQKDKPDPIENTEQENKKSETTKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTEADTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSKPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGVANGGNVVDISGSSVQ
                     GLKDEIVNGNPVVAWVTYAFEPAQHKPMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINAGSFETAYNVSQLAVSVR"
     gene            14155..14280
                     /locus_tag="ACGE70_001089"
     CDS             14155..14280
                     /locus_tag="ACGE70_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692739.1"
                     /translation="MINRCGDFNLGMGAKVITTPTYRGSFSSPNWYKEGVTRDEA"
     gene            14270..16660
                     /locus_tag="ACGE70_001090"
     CDS             14270..16660
                     /locus_tag="ACGE70_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFF8692740.1"
                     /translation="MKHEKTIILKSKIIYCFATLVMMLSTLIMPFSAIRAEADVTKPT
                     SAKFIETGVKTDGYIRVNMPNHPNEWMISSQFKDSHGNIGYCMDSELPSPTGSGAGSL
                     KYKGAGSDEFYRMFKGGFPSKTAKELGAGNDTEAWYATQLVSWVLAGNFKVSQIVWSH
                     PNHTAAETARVKKAFEKIYDYAKNGKDTPNTEFSITASKTADEGKYHTFTYKTASNKT
                     GNAKLTFTSAKPAGMKIYDADGKEITNNTVKLNSSFTIKVPVTTPSGTLSFKGTANVS
                     TTNPFTFDGRGVYQDAVVMITTSETKDSKSLSAKWTRAVGQVTVKKVDQDGNALKGAE
                     FTLTDNNNAKSVKTTGDDGLATFDLQQGLTYSLEETKVPEGYQGSFKKDGITVNTDGQ
                     EFSYTATNTIKKGKVKVKKVDQDGKPLANAEFTLTDDSSQTQAEKTDENGLASFDIVA
                     NKTYSLEETGVPTGYTGVFKQEGITVKDDGQTFEYTATNTLKKGLVKVHKVDQDGKPL
                     ANAEFTLTDEKGATQVEKTDENGLASFNIVANKTYSLEETGVPVGYTGAFKQEGITVK
                     DDGQTFEYTATNNLKKGVVKVHKVDQDGKPLADAEFTLTDEKGVKEVKKTDATGLATF
                     NIIANKTYNLEETKVPTGYTGAFKQEGITVKDDGQVFEYTAKNTLKKGVIKVHKVDDQ
                     GKALANAEFTLTDDKGVKVVQKTDSNGLATFNIVANKTYKIEETQNPNGYTGKWSKEG
                     ITVADDGQTFEYTVINHLVPETVRTGGIGTSAFLLVFLIAGLSMVLLYYREQHKAK"
     gene            16709..18544
                     /locus_tag="ACGE70_001091"
     CDS             16709..18544
                     /locus_tag="ACGE70_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFF8692741.1"
                     /translation="MKSKKIFSNLMLFGTLLGVGLGASSISSSLGLGGQVAYAQQNYN
                     YQGTGTIHIHKVTNTNGDGNPVSDIDNKGKEIALPNGVKPLEGVEFSYVKLTKDEAKS
                     LGWTDSDTTKGQHIDDAKARLLLNKKYSEVSRMKTDSQGNIDIPIQVNTDDLTDNLYL
                     IVETGTVKGVVEEAYPMIVNVPELNGSDDQYFNYDVHVYPKNYFQLNSAELDKKDEDG
                     KALAGTVFELRKDDGSDQVVGTYKTDSTGKLTVDGLELGDYYFLETDLGTDANKTTYL
                     LDGKKFKFSVKRGETAKVVLDVTNYKRPVITKVIDKETVDTGEDVQYTLKPSIPGNIG
                     NYEKYVVKDVLGAELTYDSSEVIEGMTLDTDYSIERDGQTTNYVFTESGMDKLQKQFD
                     AGDRFFTILMNAHTNSKAKMDTPLKNDVSTIYNNGYEPEDETPVDHAYTIIGDHQFIK
                     TDGDTSKALSGAKFVVKNKIDPNKVTYLKQDPTTKSVTWIADKSKATEFVSGSDGKFQ
                     IEGLQYTYDVDFDKQGNPTITDKKINDYALEEVVAPSGYTPTRNDVPFVVSNDDASSE
                     VTDIKNLKTPETVRTGGIGTVVFLVVAGVAGTGLYVVYKREQHKA"
     gene            18619..19476
                     /locus_tag="ACGE70_001092"
     CDS             18619..19476
                     /locus_tag="ACGE70_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFF8692742.1"
                     /translation="MKKKNIKLIILKIGMLLCLGVGLYPVITQAYTYYQTQQTLNSYK
                     EKYDEASEKENEEVFKRYSEVNRKRKDTEQTSVTIPEDNNNIPSNGNLQKDKLGELVG
                     EILIPKINVDLPIYDGVNDFQLSAGAGILTGTDYPTGGIGNHSVISAHSGIPNKKYFT
                     DLELLKLGDKFFIDISGKKLAYQVDRIKTILPTEFEDLLPVAGKDYVTLMTCTPVGDN
                     SHRLLVRGVSIPYQKSIVEKEKKKEDLKVKAQMNWYPIVVVGVLLIIAVIVIIKKIVA
                     KRRGMKNEE"
     gene            19466..>20566
                     /locus_tag="ACGE70_001093"
     CDS             19466..>20566
                     /locus_tag="ACGE70_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFF8692743.1"
                     /translation="MKNKILLFLTLICTLVLTIYPANQLYHQFKTKENVAEIQEKIEK
                     KQTPSKEEVAKKVEQIKSNSGNQGLDNTQKTDKGVDNTQETDGGSFDIREAEGKVNPY
                     TGDVFSKNQTKDFEPSTYSGAVGYVYIPKINVLELLFLGASQEHLYRGVAQMSATSLP
                     LSGKGHQTVIAGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRTDVIRNYGQ
                     NSTLTDIPDLDMLTLYTCHPYPQNYDRLLVRSLRDEKAKYITPQVTVGNTAYADAIDS
                     ESVSDKTETVTSSNDLFEDVDRANKELDGNNDLNIPEQGSVEMQDRTFAEKASSIFTG
                     ITNKHQKMLLVIWTVIGALASLKVLILLIKEFR"
BASE COUNT         7583 a         2897 c         4016 g         6070 t
ORIGIN      
        1 ctttatcctt taattgaaaa ccctgatcca tagtcactgc atttggatat agaagcccac
       61 tataatgttt acgtaaattc acttggaatt tctgattata aaagtctaac agattagaaa
      121 taaaaggcgg aaaattactg tttttgtaaa attgattatt tccatttagg ttgtatagta
      181 atttgttaaa ttgctcctga tttaatttat tgtaaatcca tggtaatact tccatatcct
      241 tatctgctaa cctgaatgaa ctagcatcgg taagatgttc gatcataaaa tctatgaaca
      301 tctctttttc accaaaatat aaatattgat tatatattgt ttctaatgtt gaagatggta
      361 gcgaagaaac tctcattttt tgctggagta taggatcaac tagactattt aagtgatttt
      421 gtaattcttc attgttgttt cctagaactg caagggaacg taaacggtag taatattcta
      481 tttctgttag tacttgttgt ttcggtttaa aatcatttat tgatatttgt aaattttgaa
      541 ttgtctcgtc tgaaaaatca tgttgtaatt ctggatgggt agctataaaa gtaagtaaaa
      601 agaacccttt tgtatacgtg atctgttgat tttcaccaag tgatttatta atttcctctc
      661 ttgattcagc tgtgaaccta tactttgcaa tttctttttt tagaatattt aaatagaatt
      721 ctttattatc aaaaattagg gcattaagta gtctgaaagt actttcccta tctctatctg
      781 cttcctcatt tccataagat aattgaaaaa caaacttaaa ggctgtctta cataggtttt
      841 ctttggcttt ttcgcttagt ttatttagat agctattttt tagtgcagag tgaaattctt
      901 caggtgtaac agacatagca tgtatgcgac ttgcatattg tataatttcg atcgttactt
      961 tttttcctaa tattggattt tttaggaata taatttcata ggacttccgt ataacatcct
     1021 gacaagtttc ttttgttata tctcttaatt cccaagtatt accgttgtaa gtgactacag
     1081 gatgagctga ataatttctt tgatccttaa tatattttaa atcctcatat tcatagctat
     1141 caattagctc agttgaacta taagcttttt ctagtagttc catttcccat tttggagagt
     1201 ttggatcact ttttcttagt ctagctatat cttctaaaat tttcttagaa gtattatcct
     1261 cgtaaatatt tgctaaatca gataatttat caattaaatc taaaataagc gcactgtaat
     1321 tgacagctat tgcagaacga taatttccct gataaaaact acttctaact tcttctaata
     1381 atggaataat tctaggattt ttaacttttt ttatttcatc atctaatata aacatataaa
     1441 tctccttttt ttatttgtat gacaagttta ccataaaaaa aaattaaaaa gcgagacata
     1501 tttatgttgt tgtgttatca taataacata aatatgtaga gtgagatgat gaaaaaatgg
     1561 atttgaaaca atcgttttac atctggttat atcctgataa tcctatgtca ataatggctg
     1621 atatttttgt agttagcatc ttattgtatt tattttatct aattttcttt cacaatttaa
     1681 tcgggtacat gattcttgat tggaaaggaa tgttcgatat agaaagaaaa ccatttgcta
     1741 acaatcaaac ctataccgtc ttcgaaagtg gagattggga agaaaatgaa gttgatgtga
     1801 tccatgaaat tggattagct ttatcaggaa tacaagatgc tgatgttttg gtatcaatga
     1861 caacggatac tggatacaac gcttatgagg tattttatct tatgtatttt atatggcgtg
     1921 atggtcaaga aattatcaat ggtcgtgctg aaaaaacaat caattctaat actgaaattt
     1981 ctttttgtat gggtgaaggg gaaatgattg tatataaaga tgggtcagca aacattaaaa
     2041 cagcctttgg tgatattgaa acaagtatta aggaacttag ggaatatgtg cagaacccat
     2101 ataattttca atatttagca ggaagaataa atttagttta ggagtaaaaa tatgaaaaaa
     2161 attataaatc aaccaacaaa tcacttgata attgaaggtg ttattttcgt aacaatgtat
     2221 tttactgtag gaaatggcat ctcaaataca atcattcaaa aaaatccaca atacctatta
     2281 tttagtcttt atgcttggat tgtacaaatg gtttgggttt ccatattgat gctgttgttt
     2341 tattggatag tacgggttat tcttttattg ttaaacaaaa gaaaaaagga taggtgaata
     2401 aaatgaaatc aactaaattg aaggttgtat tgttatcgac agcagcatta ataaccctta
     2461 cagcatgtgg tacaactaat agtgctaata ataacaaaga cacaaagtct agtagtacga
     2521 tggttactaa aaacagcact acatcggcat caacaacaat gtcttcaatg actgaaaata
     2581 atgctgaaaa gtatcaaaaa tacgaagaca ttaataaagg tcaaaagccg gatgaagttt
     2641 ataacaactt tattaataca gtaaaaaatg atgaaaatta tgggaatgat ccattgcaaa
     2701 ataaaaatct gactatcgtt ctttacaaac ctgattgtcc agattgtcaa aaagcagaat
     2761 caaaaatcat gcaagaaata aaagaaggat ttagttatgc tccaacatta atgcatgata
     2821 ttaccataaa taaaccagct tacgagtatt acgcagttgc tgtagatatt actgatggtg
     2881 tacctgagtg ggtacaaaac attcctggtc tggctgatga gaacaatcaa tatcatacgc
     2941 caacagttgc agtagtaaca ccaaagaaag ttaacggcac actatactgg gatgtaatgg
     3001 gaatgacaac aaatacaact gatgaaatga ttgaaagtac aactaaatat tatatggatt
     3061 tgggagcata taaattatat caacaatcag agcctgttat ggacgatcaa tccaaagtta
     3121 aatagatctt tagggcgtaa gccctatttt tttaaaatta agtgataaca aataaatgat
     3181 ttctgaaaca aacgttctta ctcaactact cgaaacacac aagctaatta actagattat
     3241 aaaggagaat gcgtaatgaa agaaatcagt gcaaatgaag ctcatattcc gcaaaagaat
     3301 caggttggta tatataaggt gcttcatggt gaaggaattt ataaaataca gttacttacc
     3361 ggttacaata atggtgcaaa tgggaagtgg ttaaaggcaa caagtattcg tgaacttaga
     3421 agtgaagtag aaaaaattag gcttggtctc acaagtgaag aaatagctaa actgaaggga
     3481 aataattctt ttgtaattga aaaagaaaag gaaaaacatc tactttatcg tgagctgaaa
     3541 attgaagaat actatgaaat tgatttacct gaaaaattaa gaattattga tcaacgttca
     3601 gaagaaaaat ggaaaactga aatatatcca agcttaaatg actacaagac atcaggaaag
     3661 cccatgtata actacacagg ctttttaacc tacacacaat acaaatatgc ctttcgcaaa
     3721 ttaaaaggca ttaaacaagc tgaaattgcc ctgtatgaga tgaaaaaagt ggatagattg
     3781 aaattgattc aagatttagt gcaaaataca tggaatcaag cgattgtcga gctagaaaaa
     3841 gaaaatggga aaattttaac taataaatat ctgatcgtaa atgaaattga agaattaatt
     3901 ggtttatttt catgtgaaaa aggtgaagtc ttcttgaagt catcaccaac ctattcaatt
     3961 ttggacagtc cgtatcagtt tatacaggca aggagatgag ttagatgtct agcttgaaag
     4021 atgactatat ccaagcaaaa caagagtatg aaaaaataca gatgttgaaa tctgagaaag
     4081 tcgaattatt tttaacacaa aaattagcag aggatcatat tgctaactta gatgctaaaa
     4141 ctttagagga atatgatcgg cagtttaatc agatacatca caatatcatt ttgcttgttg
     4201 agcaggcaaa gcgaaggttt gaaaaagcgg aatatgatct attagatcaa ggaattgtat
     4261 gcttggataa gggattgcga agatattatg gtgcagagtt aaaaaagcaa aatgtagata
     4321 gaaatgcact aaaacatgaa atcttagaat tgataatgca acgaatttga agggatagaa
     4381 agcaaatgtc acaaaacatt agtgctgtaa aacaattaat agaagaagat ttagagacca
     4441 cagttcagag attagcggaa cgaatccatt ttgcaaatta tcaaaccatc tctaactggt
     4501 caagcaggaa cagaaatccg gaagaaattc caatgaaatt tttttatcga atcggaaaag
     4561 aatatggtgt tgatccgata aaaattttcg agaagaccat gatttattac tatcatggaa
     4621 cacctaacga agtgttagaa catcttttgg aagaggcaaa agaaaaatca tcaatattag
     4681 agctatcgca aaatgacaaa gttattcaag tgaaatgcgt tgatccaaca ggaaagatta
     4741 ttgtcggttt tgatattgaa agaaaatcca aaatagattt tattacacaa ttaaaaaaga
     4801 taaataattt agatattcta acgcttagac cagatcatga acagttgttt atgagtttta
     4861 aaaaacaatt gaaagaaata caaaaaagat ataacaaggt gtttcctaat gcgtgaaaag
     4921 ctcaaagaaa tcagagtttt ttggataatt aacaaagaat gtatcatctt atatactctt
     4981 ggatttctta gcatgatctt gttttggtgg ttatatttaa aacagtaatt ggagagggtc
     5041 aagaggattt ttatcaagat aaggatgtgg aaactgcatg tggaataata gattaaaaac
     5101 aggattgtta ttaatagtta tttcctgcgc tatgatgatt ggtatgagga tacaaaggga
     5161 acaatcctat tttgaagttt ctgctaacaa tgtaatagaa agatgccatt atggtcaaca
     5221 tttttggact ggggaagtca gagaaaatat agatcgtgaa tatgttcaaa gaattgtttg
     5281 ggatgcatat tctatcaaag attatcctaa aagtttaacg agtagattat tttacagtga
     5341 aaaagataat caaaaactct cagatttgat gatgaaaaaa gtatgactat tattacgaaa
     5401 gagaaacaga gtgctatatt cgaggggaaa aagcctacaa caaaagtgac tattggggtg
     5461 acaaggcgaa gcgattaact gcaaatttta gagtgtatga atcagagttt ataaacttga
     5521 cctttcttaa tagtgttttt attgattatg taattactac gaaacgcatt ggaagttatt
     5581 caagtaccta cgcatcctta gttccaatgt taaactcgat gagccgtttc ttaaaagaaa
     5641 gagaaaagaa agagctacag tatatcaaag aaatcatgcc ggattttgaa atgcacgaat
     5701 ggcaaattgc tttatcagat tggaaattgg cgaataaggt tcgtagaata acaccttttc
     5761 aagccaagcg ctttttgaga gctatgaaat ttgtgcaata aaaaaagcca ccagacaagt
     5821 tgatggagac taacacaatt accaacagtt gtaattgtgt tagcttacaa attaattatg
     5881 gtgtttagtc aatcccaaat tgtgaaatgg caccaataat tggaaaggaa aaatgaaatg
     5941 tctgaagatt tacgagagct gaagttacga aagaagcagt atgatcagta tttttccggt
     6001 ttgttgaata aacatattga agaaggactt gatcatgatt ttggaaaaga tgaatattgt
     6061 ttatattgcg aacgttacaa tgaaaaaatg tctgaatatc tacagctgaa aaaagagata
     6121 cagaaattag agaaaaaagt acatgcttgg aagtataaag attccatgtc tggttatgtt
     6181 gaatctcaaa ataagccaag aaaaaagaag caagcgtatg ttctaaagat ggtaaatggg
     6241 gaagagttaa ttgtattagc gtacagtgaa gaagaggttc gagaactcgt tgaaacacct
     6301 gtcaggaaaa aggttgaaac aataaatgag atagacgaca gccaatacaa tagcatctat
     6361 attgaaacaa gtcatggtgg atataagaaa ttagcaactt tccttagaag ttctaaagtt
     6421 gggataattt ctaaaaaaga cataagtgct gttaccgata gtttttgtac tttatctgtt
     6481 cctaaagctt atttccaata cgatagtaaa agatttgaac gcttagagaa attctgtaga
     6541 ccgtatactg tattgaaaac taaaaaattt aataaaacta aaagagtttc tatacagtta
     6601 ccgactggtc aaatctcatt aggtgaaaaa gactatctga taaaagtagg aaaagagtta
     6661 attataaaag taccggaaca cttatttaaa gaaaattttg agatacgtga aaccttggta
     6721 tggaaagaag aaaaatagtt tggaagacat agaatggtat aaggggaaat tgaaatggca
     6781 gtaaataaaa tattgcaggt actaatcata gacgaatttg gcggaggaga atggtttgat
     6841 gtgcagtcca tagactttga agaagaagtc gtagttatag aaaatattta cggcgaagta
     6901 tattcatatt cgcttgaagg tgagaatatt gaggatttac ggttgattgg tgagtagaaa
     6961 aaaacagaaa tatcaaaatg gagataaaaa ttaatagcat gtaaagatag tcatgcttgg
     7021 gttaaaaatg ttaaaattca aaaaaatgag tcagaaggga taaaagtatg aaaagagagg
     7081 aatacaaaca aagattaaac gaactgcttg aagaggacga aacattaact catggttcgc
     7141 ctgatgaaat tctatacatg attgataata tggtgatctt tggtggatat gaacttggta
     7201 ataggtcagt tgatcataat attttagaat ttgatgatgt atcatgggaa gaaattcttg
     7261 attggggaat tttggcggta cctgaaacta aaacctatat tagtgacact atggttcctt
     7321 tttttgagga attggattat aaaagacttc ccaaaaatga aaatcatata ctgggaggaa
     7381 actaacaatg ttaagtgact tatttatttt actgatctat ctaggtgtaa gtgaaattat
     7441 tgcgtatttt ttgtttgtca ttgctaatta tggtatttcc agtgcaatcg ttcaaatgtt
     7501 tagattacca gtaagtgaac taaaaaaatg atgagattat cagcatatct tttaatgttt
     7561 ttgaatgtgg tcacgttaat ctttgtagta atcggtgtga ctttcgattt aatggaatag
     7621 tgggaaatct ataatggaaa aaagagacac gaatgaacat attttttgaa tcaatattgt
     7681 tgatggtttt tgcgatcagc gcttaccaga aatctaaaaa agagtaatga ttgtttaatc
     7741 atcactcttt ttttgtaggg aaattgaaat atgtggagct tgtattggtt tagttaggat
     7801 tgcttggcta taaggagcta gttctttatc aaaaccatca aagtaggttt tcagtagcga
     7861 tttaactgat cgctcatctt gtttgatttg gataatttca gcatccaaat cggttgtgag
     7921 atcaggagct tttgatgcga ttacctgatt ttttttatct tccaactctc ttaggctgtt
     7981 taacaagttg taaaaagaat cattttgatt gttcattcca acgtgagttg caattttaac
     8041 agaagtttcg tccgtattgt catcaaaaga actcattgga atatcaagta gtaacgagga
     8101 tgtttctttt actgactttt tctttacagc attttttgtt tttccatctt ttttgctctt
     8161 tactcgagat gtttttggag ctagtttagg taaatcactt gttttatcag tcatttatta
     8221 gcttttcctt tctattaatt tatgttatta tattatcata ataacataaa aaagtacact
     8281 ttttgggggc gtagttattg aaaaaatcta agagaaagat tgccacattg cttatgttaa
     8341 tggtgcaatt tacagggatt gcaatgagtg taatcacaat aaatccgcaa gtgatacatg
     8401 ctgatgggat agctacttat gatgcacaat ttaactgtac tactgaaggt agtgggtcag
     8461 gaacagatag cgatggaaac gatactggaa gtggcgctgt aacaggtgat gtagccaaaa
     8521 atattgaaga tatttacaaa tacatgaatg gagaatatgg cttatcaggt gctagtattg
     8581 caggtatttt aggtaactgg aaacaagaat caggtattga tcctcgagca gtagaagggt
     8641 attacggaga tggacagaga gaagttgact tcatcaaaca atttactgat ggtctgatgg
     8701 gtattggcta tggtcaatgg acagctgggc gacatacagc attagttgat tggtctaaga
     8761 aaaataatgg cggttcttgg tggactacag aagcccaatt ggactttatg ttcaaaggtg
     8821 atgggggatt cgtaggaata ttaaaagatt atgctttaaa tgcttctgat gatgtcgtag
     8881 aaaatgcagt aacttttcat aaaaactggg aaatttcagc tgattccgaa tcagctgtaa
     8941 tagctgtacg tggtgaaaat gcaaaagaaa tctggaagta catgaaaagc aagggtatgg
     9001 acggtgcaaa agatgaaagt aagatcaata aaatatccgg aggttcaagt accggagatg
     9061 gatcgaataa ttcatcaagt tcagcatcag gaacagtaga agatgcctgt gcaactacgg
     9121 atgacgctaa ttctggttct ggatcaattg ggaaagaagg aaaaattaac ggtaaaaaag
     9181 gtcaagtaat agcgaatatg acttatgaag aagctttgaa aaaatatggg aaagcgatca
     9241 cgctaccaaa attcaaagaa ccagattggt caacttcacc ttttaacgga atatatcaag
     9301 gtcagtgtac agagttgacg tgggcttata tgagccaatt atacgatggg aatcaaccga
     9361 ctactggaaa tggtggttat gtatgggaaa gctacaaatc ggcaggagca aaaataacga
     9421 acaaaccaat cgtaggttat gggtttagtg cttcggatgg atggttatgg gctgttggag
     9481 atgcagggca tactggtgta gttatagcgg tgtttgaaga tggatcattt ctatgtgcta
     9541 actttaatgt tccaccaacc caagctccaa caaggggagt ttcggtaacg ttgattgatg
     9601 gagtggacgg atcagataat gtgcatttct ttagcggtgt aggaaaagct aaatttgaaa
     9661 tcgaaaaata aattggaatg aaggacgata tagatgaata gtaaagtgaa agatacaatt
     9721 atcatttctt tgctttctgg tatgttatta gcactgcttg ttggaatcct attcttcaat
     9781 aagcagaatc aggacataga agctgaaaaa cacagtgtgc atattgaaga taagctgaca
     9841 ttctttaaag aggttaaacc aaatgaggtg gatggattac ggaaagattt agaaaatgat
     9901 attcaagaaa tgttgaatgg atcagttcaa aataagtttg cagatcaatc aggtgaagaa
     9961 tttttgagaa aactatttat tgtaacaggt aaaccaccaa ttactgataa gagtagtgag
    10021 aaagaaaaaa tcgagtacta taagcctttt acttttaagc taacaaactt tggaattaag
    10081 acatctagta gtgggttgta tgaagcattg accgattttg aagttcaata caatggaaaa
    10141 aaagtgtatg ataccacatt aaccatcaca ctaaataatg ataagaaagt gactgggggt
    10201 gctttttatg caaaatcatg aaatcactac tgctaaaaag gcgatcggaa tactttccat
    10261 aattgtgatc ctttgttgtg tggtactatt tcaaaagaaa aatgcttatg ataataatgt
    10321 caaagaattg gaagacctta aaagtaaaca aatcgaagta gtcaagaata accagaaagt
    10381 gaaagctatg caggaagctc aatacaaaga attgggaaat gctgaaatta aggaacaaag
    10441 taggaaattc tttaatttat tttacaactg gaattcatgg gaaaaatata cgaagaatat
    10501 gaaggaaata caaaaagaat ttcctcagat agtaaagtct agtcctgttg atatttctgg
    10561 cacgatggta ggcactggta atagtccaat atctagctat actgctgatt actatacaac
    10621 aaatcaaaaa ggtaaaatgg ttgaattgat tacacagaca agaaatactc caacaacagc
    10681 aacaacaaca atttggcgtg gagtaacaag cataggagaa aatcaattgt tttatttgga
    10741 ggatttagat agctatgtaa gagcagagta aaataaaaaa agagctaacg gtaccaacgt
    10801 tagctctttg tgctgacgat gataacaaaa cctaccgaaa aaggattgtt tcatcacaaa
    10861 cacaaacaga aaagaattga taaaaaaccc gaacctcaga atttttatta atcaattcat
    10921 ttagattata ataaattgag ctgaacatta caatacgtat atgttgtaaa acgtaaaaaa
    10981 agtcaaaagt aaatactttc ttaaaaagtc aaataaaact attaaagaca atatatatgg
    11041 tttatttgac ataagaaaat ggtctagatg ataattgtaa tatagaattg tagacttaaa
    11101 taaagcataa aatacctttt atgataaggt atagctctta attttaagca gttttagata
    11161 taagattgaa agtaaagtgg gggagaataa taatggcaga tcataaatta gaaaagttat
    11221 tagaagaaac gtggaatcca aaagaattta gtgaattttt tatggagaat tttgaaactg
    11281 acttagcagt gattgttaaa gatgctctta gagagcaagg gtatcctgaa actgcaaact
    11341 atataaacat taattttact ttgtatactg aaaataaagg aacttgggat ttttgggcta
    11401 ctttagcaaa taaggagtta agtgataaat cagacacagg aattcgtaac ttttttgaat
    11461 caaacagaga tgattatatg tatgctaaca atcaaaataa attgaacttt agagtagagt
    11521 ttgatgaaac acctgaagaa tttatagaaa gacaaccgcc taaagaaaat gtagcaaagg
    11581 tattggaaga tcgttggaat tcagatgaaa tagtttcaac aattagcgaa ctaggcggac
    11641 aatatgaacc gctagtagaa gcggttagag aagagctaag actgaataag tttcctgatg
    11701 ttcaaaatat tgatgtttct caaattgaaa taaacgtcaa gatcacgaat aaacttgatt
    11761 atggtagttg ggcagatata gcattagaaa aatacatcta ctcaacgtta aaagagttta
    11821 tcgaaaatag aatggatatc atgtatctac agcatccaca gtatttaaac tttggtgttg
    11881 aaatagctac acctcttgaa gaatggaaaa tggagcaagg gcttgactag cctttgctcc
    11941 atttttttat tttacaaatg aatacagttc gtcgttttta ccaagaatga ttgtttgccc
    12001 aactttgaga ttgtcaggtg atccttcagc ggtttgtaag gagaacgttc cattgaactt
    12061 atcggatacc gttataggta gctttaattc tgatccttcc tcatctttga gaattgctgt
    12121 ttttcgttca tcaaaataaa cagtctttat taaataattt ttcgtagtga tctcagcaga
    12181 tttgtatttt tttgtagtag taccagtttc tttagaatta gaggtatttt ctacgactat
    12241 tccatctttg gcatttgagc ttgtgtttat ttgattgaat attttcagcg acaagataac
    12301 gctaagaata gtcaatatag aaacaagtgt aattaatact gataatttcc tttttttgtt
    12361 cattttttct taacatcctt ttttgttttg attagttaga tattccttat ctaacaagcc
    12421 tttttaggct attaaatgaa gataaacccc aaaaaatatt gcaattatga cagtgcaatg
    12481 gtactattat tatacaataa attttgcgaa aggggtaggt ctaaatcgca aacaaaaaga
    12541 aattgaacct agacaaatac aacttgtata ccgcaaggga agcaagtttg attttgggta
    12601 aaggtagaaa ttatgttaca aacataatca actattatcc aaatcgtttg cctaaaggtt
    12661 cgttcaagaa agtgggtgac aaaacctata ttataacgga agaaggaata gaagcattgc
    12721 gatcacaaac agaaaataaa aacaaaaaga aggaatgaat atgaagagaa attcgcttgg
    12781 tatggtttta ttggcatcag ctgtacttgc ggggttatca gtaccagtaa cctcacatgc
    12841 tgatgaatgg actgcaaaca caccggatca aatccaaatt acaacaggac aatcaagtta
    12901 tgcgttcaaa aaaggagata ctatttggtc aactgtagag aagcttaaaa gcttaggtta
    12961 caagactact gttgctgagt ttgccgaatt gaacaatgtt gatttggcaa aaggagaaca
    13021 acggactatc ccgattggac gaactttcaa catcagtaaa gatacattaa cagttaagga
    13081 agcagatggc gaagtagtta gtcaaactgt tcaaaaagat aaacctgatc ctattgaaaa
    13141 tacggagcaa gagaacaaaa aatccgagac tacaaaagaa tcggaaccaa cagcaaacac
    13201 ttctgataag acagaaacaa gtgcttcaag tgttgatgga gcaagttcat ctaatacaaa
    13261 taattctaca agtgagaaag aagcatcaca gggaacaagt gaaagtagca ctacggaagc
    13321 tgatactgga acatcaacaa ataactcaca atcaacatca aatgatgcaa atgaatcaac
    13381 cagtacatct tctagtgatc aatcacaaac aggttcgagt gatgaaactg acagttctac
    13441 ttcaccaagt aaacccgaac aaccaactaa tgttgtgcta ggcgttccat ttattagtca
    13501 aggtaacaca atgctttgcg aaggaacatc tttgctagaa gcgttacatt ataaaggtgt
    13561 tgcaacaaat caagatttaa tgacttttgt taaatcaatg ccactatctc ctgacaataa
    13621 tccaaataat ggtttttctg gtgaatggag acacaatgtg gatggaactt atcaaggtat
    13681 gaatccggct cctgtagttt catggggtgt tgctaatggc ggaaatgttg tagatatttc
    13741 tggttcatcg gtacaaggat taaaagatga gattgtaaat ggaaatcctg tagtagcgtg
    13801 ggtaacttat gcctttgaac ctgctcaaca taaaccaatg ttttggggcg atgctatttg
    13861 gaatcgacat gtagtagccg ttgatggatt taaagacggt ttttaccatg ttgtcgatcc
    13921 tgtgtttggg gcaagttgga ttaatgcagg atcatttgaa acagcatata acgtatctca
    13981 attagcggtt tcggtacgtt aataaaagat atcgtttata tactaagtag ttatatagtc
    14041 tttatttgtc cttgcttaac tacttagaaa acttgttgct catggtgggt agaactaaaa
    14101 ggggtgatgc tatgaatact agagcgttga aacagcttgc tttcaacgaa aaaaatgatt
    14161 aataggtgtg gtgattttaa cttaggaatg ggcgcgaagg tcattaccac accgacatat
    14221 cgtggctcat tttcttcgcc taactggtat aaagaaggag taacgagaga tgaagcatga
    14281 aaaaacgata attttaaaat caaaaataat ttattgtttt gcgactttag taatgatgtt
    14341 atctacgctt ataatgcctt tttcagcaat aagagctgaa gcagatgtca caaaaccgac
    14401 aagtgcaaaa tttatagaaa ctggcgtaaa aacagacggt tatattaggg ttaatatgcc
    14461 gaatcatcca aatgaatgga tgatttcaag tcaatttaaa gattctcatg gaaatattgg
    14521 atattgtatg gattcagaat tgccatcccc aacaggaagt ggagccggaa gcttgaagta
    14581 caagggagct ggcagtgatg agttctatcg tatgtttaaa ggtggttttc catcaaaaac
    14641 agcaaaggaa ctgggtgcag gaaatgatac agaagcatgg tatgcaacac aattagtttc
    14701 atgggtttta gccggaaact ttaaagtttc acagattgta tggtcacatc caaaccatac
    14761 cgccgcagaa acagctcgtg taaaaaaagc atttgaaaaa atttatgact atgctaagaa
    14821 tggaaaagat acgcccaaca ctgagttttc tatcacagct tctaagacag ctgacgaagg
    14881 aaaatatcac actttcacat ataaaacagc atcaaataag actggtaatg cgaagctcac
    14941 ttttacatca gcaaaaccag ctggtatgaa gatttatgat gcagatggta aagaaattac
    15001 taacaacact gtgaaattaa attcaagttt tactattaaa gtacctgtga ctacaccaag
    15061 tggtactttg tcatttaaag gtactgctaa tgttagcaca accaatcctt ttacttttga
    15121 tggacgaggt gtgtatcaag atgccgtagt tatgatcact acttcagaaa caaaggactc
    15181 taaatctttg agtgctaaat ggacaagagc tgttggtcag gtaacagtta agaaagttga
    15241 tcaagatgga aatgctttaa aaggagcgga gtttactcta actgacaata acaatgctaa
    15301 atctgttaag acaaccggag atgatgggtt agctacgttt gatttgcaac aaggcttaac
    15361 atacagctta gaagaaacaa aagttcctga aggttatcaa ggttcattta aaaaggatgg
    15421 tattactgtt aataccgatg gacaagaatt tagttacact gctacgaaca caattaaaaa
    15481 aggtaaagta aaagttaaaa aggttgacca agatggaaaa ccattagcta atgctgaatt
    15541 taccttgacg gatgattcat cccaaacaca agcagaaaaa acagatgaaa acggacttgc
    15601 aagcttcgat attgtagcaa acaagaccta cagccttgaa gagaccggag taccaactgg
    15661 ttatacaggc gtattcaagc aagaaggaat taccgttaaa gatgatggac aaacttttga
    15721 atatacagct acaaacactt tgaaaaaagg tttagtaaaa gttcataaag tcgatcaaga
    15781 tggaaaacct ttggcaaatg cagagttcac tttaaccgat gaaaaaggtg caacacaagt
    15841 agaaaaaaca gatgaaaatg gacttgcaag cttcaatatt gtagcaaaca agacgtatag
    15901 cctagaagaa accggagtac cagtaggtta cacaggtgca tttaagcaag agggtatcac
    15961 cgttaaagac gatgggcaaa cttttgaata tacagctaca aataatttga aaaaaggtgt
    16021 agtaaaagtt cataaagtcg atcaagatgg aaaaccttta gcagatgcag aattcacctt
    16081 aacggatgaa aaaggtgtaa aagaagttaa aaagacggat gcaactggtc tggctacgtt
    16141 caacattatt gcgaacaaaa cttataactt agaagaaaca aaagtaccaa caggttacac
    16201 aggcgcattc aaacaagaag gaattaccgt caaagatgat ggacaagtct ttgaatatac
    16261 tgctaaaaac actttgaaaa aaggtgtgat caaagttcat aaagtggatg atcaagggaa
    16321 agcattagca aacgcagaat tcactttgac agacgacaaa ggtgtaaaag ttgtacaaaa
    16381 aacggacagc aatggcttgg caacatttaa tattgttgcc aacaaaacgt acaaaatcga
    16441 agaaacacaa aaccctaatg gttatacagg gaaatggtct aaagaaggca tcaccgttgc
    16501 tgacgatgga caaacctttg aatatacagt aattaatcac cttgtacctg aaacagtaag
    16561 aacaggcgga atcggtacat cagccttcct tcttgtattt ctaattgcag gattgagtat
    16621 ggtgctactg tattatcgag aacagcacaa agcaaaataa aaaagtgtta atggaaaaga
    16681 agtaaatatg aaaaaagaag gctgacgtat gaaatcaaaa aaaatttttt caaatttaat
    16741 gctatttggt actttgctag gagtgggatt gggcgcaagc tcaatctctt ctagccttgg
    16801 actagggggg caagtagcat atgcacaaca aaactataat tatcaaggaa ctggaacgat
    16861 tcatatccat aaagtaacaa atactaatgg ggatgggaac cctgtctcag atattgataa
    16921 caaagggaaa gaaatagctt taccaaatgg tgtaaaacct cttgaaggcg ttgagttctc
    16981 ttatgtgaaa cttactaaag atgaagctaa atccttggga tggacggata gtgatactac
    17041 aaaaggtcaa catattgatg atgctaaagc acgattatta ctaaacaaaa aatacagtga
    17101 agtaagtaga atgaagactg attcacaagg gaatatagac atacctatac aagtaaatac
    17161 agacgattta acagataact tgtatttgat tgttgaaaca ggaacagtca aaggtgtagt
    17221 tgaagaagcg tatcctatga ttgttaacgt acctgaacta aacggatcag atgatcaata
    17281 ctttaattat gatgtacatg tctatcctaa aaactatttc caattaaact cagcagagct
    17341 tgataaaaaa gacgaagatg gaaaggctct tgccggaact gtttttgaat tgcgaaaaga
    17401 tgatggatct gatcaggttg ttggaactta taaaacagac agcacaggta aattaactgt
    17461 agatggtttg gaattaggtg actattattt cctagaaacc gatttgggaa cagatgctaa
    17521 taaaacaacc tatttactag atggtaaaaa attcaaattt agcgttaaac gtggggagac
    17581 tgctaaagta gttcttgatg ttaccaacta caaacgacct gtaattacta aggtaattga
    17641 taaagaaacc gttgatactg gcgaagatgt tcaatataca ttgaaaccat caattcctgg
    17701 taatattggt aattacgaaa aatatgttgt caaagatgtt ctaggtgcag agttaactta
    17761 tgattcatca gaagtgatcg aaggaatgac tttagatact gattacagta tcgaacgtga
    17821 cggacaaacg acaaactatg tctttactga atcaggtatg gataagcttc agaaacaatt
    17881 tgatgcaggg gatcgcttct ttacaatttt gatgaacgct catactaata gtaaagctaa
    17941 aatggataca ccacttaaga atgatgtttc aacgatctac aacaatggat atgaacccga
    18001 agacgaaaca ccagttgatc acgcttatac aatcattggt gatcaccaat tcattaaaac
    18061 agatggagat acaagcaaag cattgagtgg agcaaaattt gtagtgaaga acaaaattga
    18121 tccgaataaa gttacttacc taaaacaaga tccaacaacg aaatctgtta cttggattgc
    18181 agataaatct aaagctactg aattcgtttc cggatcagat ggtaagttcc aaattgaagg
    18241 cttgcaatat acctatgatg tagattttga taaacaaggt aatccaacaa tcacagataa
    18301 aaaaatcaat gattatgctc ttgaagaagt tgttgctcct tcaggatata caccaactag
    18361 aaatgatgtt ccatttgtgg tatcgaatga tgatgcttct agcgaagtaa cagacatcaa
    18421 gaaccttaaa acacctgaaa cagttcgtac aggtggaatt ggaacagtag tatttctagt
    18481 ggttgcaggt gtggcaggaa cagggctata tgttgtctac aaacgagaac aacacaaagc
    18541 ttaatgaaat aaaattccct agagtgtttt tggctctagg gtttcttttt aaaaagtgtg
    18601 tgaaaagtag gtatgagcat gaaaaagaag aatatcaagt tgatcatctt aaaaattggg
    18661 atgttgctat gtttaggagt aggtctatat cctgtaatta ctcaggctta tacctactac
    18721 caaactcaac aaacactaaa ctcctataag gaaaaatatg atgaagcttc tgaaaaggag
    18781 aatgaagaag tcttcaagag atattcagaa gttaatagga aacgaaaaga cacagaacaa
    18841 acctcagtca cgattcctga agataataac aatattccgt caaatggaaa tcttcagaag
    18901 gataaattag gtgaattagt gggtgaaatt cttataccaa agattaatgt ggatttgcca
    18961 atttatgatg gtgtaaacga tttccaacta tcagcaggag caggaatatt aaccggaact
    19021 gattatccaa ctggtgggat tggaaaccat tctgttatat cagctcattc aggcattcct
    19081 aataaaaaat attttacaga tttagagttg ctcaaacttg gtgataaatt ttttattgat
    19141 atttcaggga aaaaacttgc ttatcaagta gatcggataa aaacaattct accaaccgaa
    19201 tttgaggatt tgcttcctgt ggcaggaaaa gattatgtaa cacttatgac atgtacacct
    19261 gttggagata attctcatag gttactcgtt agaggggtta gcatacctta ccaaaaaagt
    19321 attgttgaga aagaaaagaa gaaagaagat ttaaaggtaa aggcgcagat gaactggtat
    19381 cctatcgtag ttgtaggggt tttattaata atagctgtga tagtcattat taaaaaaata
    19441 gtagcaaaga gacgaggaat gaaaaatgaa gaataaaata ctactttttt tgactcttat
    19501 ttgcactttg gttttaacaa tttatcctgc aaatcagctc tatcatcaat tcaaaacaaa
    19561 agagaatgtg gcggaaatcc aagagaaaat tgagaaaaaa caaactcctt caaaagagga
    19621 agtggcaaaa aaagtcgaac aaataaaatc gaacagcggt aaccaaggat tagacaatac
    19681 tcagaaaaca gataaaggag tagacaatac tcaggaaaca gacggtggat catttgatat
    19741 tcgtgaagct gaaggaaagg tcaatccata cacaggagat gttttttcaa aaaatcaaac
    19801 gaaagatttt gaaccatcta cctattcagg agcggttggt tatgtctata ttccaaaaat
    19861 aaatgtgcta gagcttttgt ttctaggcgc ttcacaggag catttatata gaggtgtggc
    19921 acaaatgagt gctactagtc tacctttatc aggtaaaggt catcaaacag taatagcagg
    19981 tcatcgtggc ggttatacgt caccacaatt tctttatgtt gatcaattag tagccggaga
    20041 ccacatttat atagattatc ttggtgaaaa agcagtttat acggttttcc gaacagacgt
    20101 tattagaaat tacggtcaga attcaacact gactgatatt cctgatttag atatgttaac
    20161 actttatact tgtcatccat atcctcagaa ctacgatcgt ttattagtta gaagtctaag
    20221 agatgaaaaa gcaaaataca tcacgccaca agtaacagtt ggcaataccg catacgctga
    20281 tgccattgat agcgagtccg tttcagataa gacagaaact gttacatctt ctaatgattt
    20341 atttgaagat gttgatcgtg ccaacaagga attagatgga aataatgatc ttaatattcc
    20401 ggaacaaggt agcgtagaga tgcaagacag aacgtttgct gaaaaggcaa gttcaatatt
    20461 tacaggaatt accaataaac atcagaaaat gcttttagtc atttggactg taataggagc
    20521 attagcaagt ctaaaggttc ttattctttt aatcaaagag tttcga
//
LOCUS       DAXWOO010000038        20318 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.038, whole genome shotgun sequence.
ACCESSION   DAXWOO010000038 DAXWOO010000000
VERSION     DAXWOO010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.038"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(63..1106)
                     /locus_tag="ACGE70_001094"
     CDS             complement(63..1106)
                     /locus_tag="ACGE70_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFF8692744.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(1483..2448)
                     /locus_tag="ACGE70_001095"
     CDS             complement(1483..2448)
                     /locus_tag="ACGE70_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFF8692745.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            2682..3527
                     /locus_tag="ACGE70_001096"
     CDS             2682..3527
                     /locus_tag="ACGE70_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFF8692746.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            3718..4905
                     /locus_tag="ACGE70_001097"
     CDS             3718..4905
                     /locus_tag="ACGE70_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFF8692747.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            4911..5267
                     /locus_tag="ACGE70_001098"
     CDS             4911..5267
                     /locus_tag="ACGE70_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFF8692748.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            5269..5607
                     /locus_tag="ACGE70_001099"
     CDS             5269..5607
                     /locus_tag="ACGE70_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFF8692749.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    5683..5939
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6002..7036
                     /locus_tag="ACGE70_001100"
     CDS             6002..7036
                     /locus_tag="ACGE70_001100"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFF8692750.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            7029..7715
                     /locus_tag="ACGE70_001101"
     CDS             7029..7715
                     /locus_tag="ACGE70_001101"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFF8692751.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            7739..8587
                     /locus_tag="ACGE70_001102"
     CDS             7739..8587
                     /locus_tag="ACGE70_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFF8692752.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            8784..9557
                     /gene="sufC"
                     /locus_tag="ACGE70_001103"
     CDS             8784..9557
                     /gene="sufC"
                     /locus_tag="ACGE70_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFF8692753.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            9570..10856
                     /gene="sufD"
                     /locus_tag="ACGE70_001104"
     CDS             9570..10856
                     /gene="sufD"
                     /locus_tag="ACGE70_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFF8692754.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            10853..12088
                     /locus_tag="ACGE70_001105"
     CDS             10853..12088
                     /locus_tag="ACGE70_001105"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFF8692755.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            12075..12545
                     /gene="sufU"
                     /locus_tag="ACGE70_001106"
     CDS             12075..12545
                     /gene="sufU"
                     /locus_tag="ACGE70_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFF8692756.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            12550..13941
                     /gene="sufB"
                     /locus_tag="ACGE70_001107"
     CDS             12550..13941
                     /gene="sufB"
                     /locus_tag="ACGE70_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFF8692757.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(14036..15178)
                     /locus_tag="ACGE70_001108"
     CDS             complement(14036..15178)
                     /locus_tag="ACGE70_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFF8692758.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(15217..15951)
                     /locus_tag="ACGE70_001109"
     CDS             complement(15217..15951)
                     /locus_tag="ACGE70_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFF8692759.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            16183..16458
                     /locus_tag="ACGE70_001110"
     CDS             16183..16458
                     /locus_tag="ACGE70_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8692760.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            16556..18553
                     /locus_tag="ACGE70_001111"
     CDS             16556..18553
                     /locus_tag="ACGE70_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692761.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(18629..18964)
                     /locus_tag="ACGE70_001112"
     CDS             complement(18629..18964)
                     /locus_tag="ACGE70_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFF8692762.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            19091..19306
                     /locus_tag="ACGE70_001113"
     CDS             19091..19306
                     /locus_tag="ACGE70_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFF8692763.1"
                     /translation="MKKVNNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            19343..19705
                     /locus_tag="ACGE70_001114"
     CDS             19343..19705
                     /locus_tag="ACGE70_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFF8692764.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            19751..20077
                     /locus_tag="ACGE70_001115"
     CDS             19751..20077
                     /locus_tag="ACGE70_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692765.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
BASE COUNT         6760 a         3540 c         4204 g         5814 t
ORIGIN      
        1 aaaacagttg tgattcggac attgttccat gcgagaatca caactgtttt ttcatgttca
       61 ttctaaactt tgatcagcca aataaatagt aaagccatca ctgtttggag aagcccgaca
      121 gctaatccat aagataagaa gcgtgtacct tctttcatga attttgcaaa gtctaaacgc
      181 agaccaattg ccgctaaagc agtgatctca aaccagctgc tgataaaatg tgccgattgg
      241 tcaataacta caggtaaaga aaacaagctg ttgaccaaac aaaagatcac aaaccctaat
      301 acataccaag gaagcggtaa ttttcgtttt ttctgcgtat cagttggcgt ttcaaacaga
      361 gctggttgtt tcttgtgcat gaatttttca aacagataga caacaacaac gagcatcata
      421 atacgtagaa tcttaaatag catggcgaat tcgacaacag aattgtcaag taagcttgcc
      481 ccagcgacta cttgaccaac agattggagt gtaccaccca aaagtgcact tttttcaatg
      541 agatttccac caaacaactg taatccaaga aaagggagag tcaacatcat cacagtaccc
      601 agtagattga ctagtgtgat gatctgtcct ttttcttcct ctttcgcatg gatggaaggg
      661 gcgattgaag caatagcaga agaaccacaa acagcgtttc ctccagccat catcaaagaa
      721 gaagtatcgc tgaattgcag ttttttacca atcaagtaag caccgatgat agtgactgac
      781 atttgcagta acacaaaaac aattcctttt acaccaattt gagcaatcgt ttgagctgtg
      841 acagttgcac ctagtaaaaa aacagagaat tctaatagtt tgctttcagc aaattttgtg
      901 ccgattgcta gaacaggctg tttaaaaaaa gtatttccta ataaaatacc caataaaata
      961 gcaatagtcg ctccacctaa ctgtggcaga taaatagcaa gaagcctgct gaaggcagcg
     1021 atggcaaaag aaagcagtaa accgggtaag atttcgcgga ccgctgaatc ctttacagat
     1081 tttattggat gtgacaatga tttcatttga tcggttcctt tcgtcaataa atactaaaag
     1141 catactcgaa catagtagca ctaaaccagt attttgacaa aagaacaaga ggtcgtgaca
     1201 aaagtcttgt acgacctctt gtttcgaata aacggtgttc cagaagtagc ttctacggaa
     1261 ataagccgaa atttcacaaa aatttgagaa gcaattttcg taaattcctt cttatttctt
     1321 gaagctgacc acttctttta cgacctcttg gtaaacggtg ttcaaaagct tgcatctgcg
     1381 gtaataagcc caaccagtac aaaaacatga acagctgttt ttgtttggcc gttcttatta
     1441 cttgatgcag aacagctttt tcacaaccta ttgaatcgat tattaagcga tttttttaga
     1501 aacaaatttt ccatgtttgc tatgttcttt aattaagaaa cgtacgtaat gagcaagaac
     1561 gtagaagaca actacacttg caaacagaca ttccgcaact tttaagaaat ccaatacgct
     1621 actatagata cctaaactgt tgaagtaatg aacagtagta aatatagctg ttgcagagat
     1681 aacaagcggg aatgtaaagg ctgcatagct tggataaaaa cgaagcttga acaaacgtgg
     1741 aagcatagac agcacaacga aatacaatac ttgagataaa gcgaacaatg ctacgacaag
     1801 ataaaaatta ggttcagcaa agtttttaag ataacctgtt aggcaaagag aaccaggtgc
     1861 tgcaacgatc gtaatcagcg gacgtgtcgg atgagccata tttcttacga aaaagacacg
     1921 tttgatgatg atcggcaata agaacgcata gcagatcaat gaaatccaaa agaaaatttt
     1981 tccaataagt gggaagaagt tcccggcagt tacagtgatg accccaaatc caacgtaaat
     2041 gataaaccag cttggataaa ttgcttcaat tgaaagatca gctttgataa catggtgata
     2101 attgaaataa aagacaagga tcacttggaa gataacagcg cacagccaaa tgaatttgac
     2161 aattggtgca atactttcat atgaaacaaa ataagtacaa ataaccatga gtgacatcgt
     2221 aaaagttggt gaaacggagg cgatgattgg atcatgcaac gtttgtttcg tgtgttcgaa
     2281 taagataaac aatttaacaa aaatgaatat cataagaatc atagagatac cgccagttag
     2341 attgcctaaa gcaacatggt gatagtcttt aaacagattt ccaagggaag ctaacccaag
     2401 gatcaacccg cagatcggga tcggaataat tttcaaaaag ttcttcatat ataatattct
     2461 cctttttatt taatagattt tagtaccaag atgaacgaat acattgtaaa atgaatagga
     2521 atgtatgatt acgagcaagc agacagctga ttgtcttcat ctaagttact agttggatca
     2581 gcaactgttt tgttgtacat ttctattata gatggttttt tgtatattta aatcaaatag
     2641 tttttatagt aattatcata aaaagttatg gaagtggtgc catgtttaag ttattaacga
     2701 cttttcgagc agtatatgaa acaagaaatt tttccagagc agcagaagta ctgtttttat
     2761 ctcaaccagc agtctcgaat caaatcaaac agttggagcg ggaattgaat atcgatcttt
     2821 ttcaacgtaa cggaagaaaa gagatgcttc caactaagca agccgactta ttatatgaac
     2881 ggacactggt tttgttagaa gaatgggaag aaactcagca aaaattgctc aatgaacaaa
     2941 atgaagaaga agtatgtcgg atcgttgctt cccacacctt tgctgtctat cttttgcctc
     3001 agttgatgaa acatctgatc aaaaggtttc ctaaagtgca ttttgagatc gaaatcacga
     3061 attctcatga atcattagaa caagtagcga aacatgaatc cgattttggt tttatcgaaa
     3121 agccattaga gacttccggc gtacaaagat actctttgat ggaagaccag ctagtcatcg
     3181 ctggggaccc aaaaagcaaa ttgtggcttg taagagaagc aaactctggt gtttttcatt
     3241 acacgcagcg ttattttgaa gagatgaata tccaagataa gaagatgatc gtgaaaagta
     3301 acgatgtaat tgttgcgttg ctgaaggaag gaatcggtcg ctcgattttg tctaagcgct
     3361 ctgttccaga aaatgtgcca atgactccgt tgaatttcca tcgatatttt tatttcatcc
     3421 aacgaaccaa tctggtttct gatcagctgc tagagattgc cgaagagatc cttcgataca
     3481 atgaaagcac agaaacttct cacagcgagg agaatctcag ctggtagata attgcatgta
     3541 tcaaaaaaag ttctatgata cactaaagat aagcagtagg cttttgtact gcatttgctc
     3601 aagcaactgc acaaacggaa agttaatttt tcccaaatgt ctatggaaag actttccatt
     3661 atctaactgg accgctataa taatcagcag tatggttttt aagaaaaggg atgaaacatg
     3721 gaaaaaaaat taaaagcttc gaacgataat cgtattgatt atggcgtgat tttaccagtc
     3781 tttctgttat gtttgattgg tctgctctca ttatatgtag cacttagcca tgatccgaac
     3841 aatccagcag tcttgaaagg cgtagggatg caagttgtct ggtatttagt tggagcggca
     3901 gcaatcgttg tcatcatgca tttgaattcc aaatggatct ggaaactgac tccttacctt
     3961 tatggtttgg ggttagtggt aatgggactc ttgctcaaat tctatgatca gaatttagct
     4021 gcttcaacag ggtctaaaaa ctggtttcga ttcggtgcgt ttactttcca gccagcagag
     4081 ttgatgaaga ttgcgtacat cttgatgatg gcactgatcg taactaaaca taatacgcaa
     4141 gtaaaagaac ggacgatgaa atcggacttt tggctgattg ggaagctctt gatcgtgaca
     4201 ataccggttt tagcattgat catggcccaa gatgactttg gaacgatgct tgtctttctt
     4261 gcgatctttg gcgggatttt cctgatgtct ggaatctctt ggcgcatcat cgcacctgta
     4321 gtaatcttgg cagtagtcgt tggagcagga acgatcttct tggttacgac tgaaggcgga
     4381 agagacttgc tttataaagt tggattcaaa tcttatcagt ttgcacgtat tgattcatgg
     4441 ctggaccctt tccatgatac gtcaggaatg agttatcagc cggcccaagg acttttggca
     4501 attggtaccg gtggcttgtt tggtaaagga ttcaacgtaa gcaatatcta tgtgcctgtt
     4561 cgtgagtccg atatgatttt taccgttatc ggtgaaaact tcggctttat cggtggtgca
     4621 ttcgtcattt tcttgtattt tattttaatc tatcgtatga ttcgggtgtg tttcgatacg
     4681 aataatgaat tttacgccta tatcgcaagc ggtttgatca tgatgctgct gttccatgtc
     4741 tttgagaata ttggagcaaa tatcggatta cttcctttaa cagggatccc attgcctttc
     4801 attagtcagg gaggatcctc gattttaggg aacatgatcg gtatcgggtt gatattatcg
     4861 atgagatacc aaaatgaagc acctgcaaga tcttcaagga ggtaagaaaa ttgtacacct
     4921 tttactggta cccgaaatgt tcaacatgca aaaaagccaa agcgtggtta gaccagcatg
     4981 gcgttagtta tcaaaccgtt gatatgatca aagaagtacc gaaggcctca acgattgaga
     5041 cttggctaga aacgagtcaa gtaccaatcc gtcgcctgtt caacacaagc gggatgaaat
     5101 atcgggaact tggactgaaa gatcaagtag gcgagtttac ggcttcaaaa gcgagccaag
     5161 tcttatcgac agatggtatg ctgatcaagc gaccactgat cttacgagag gaccagcttg
     5221 ttgcaattgg attcaatgaa aaaatttatg aaggagaact gatataaaat ggcaggcaag
     5281 catttgaaga aaaaggacaa cctttggatc ttatttaatg gaaaagaata ctgtgttggt
     5341 ttgacaaatg aagcgcaaga agaacttgga gacatcacgt ttgccaatct gccaaaagca
     5401 ggacaatcgc taaaaaaaga tgaacctctg atcgaagttg aagcggaaaa agcggtcaat
     5461 gaatttgcca gcccgctaac gggtacagtt tcttctgtta atgataaaat cggtgaagat
     5521 atcaatatct tgaatgattc agatgaaatg aatgcgtgga tcttaagttt caaagatgtt
     5581 gatccaaaag aatttgacga attataaaat cagaaaatca cttgaaaaaa gttgacactg
     5641 attcttccag ttgctataat cataagcaat cctatatgaa acgagaaagc tttgaaaaga
     5701 agagtacatg tcacggacgt ttgaagagag ccctgtttgc tgaaaatggg taacgaaaat
     5761 gagatggaag atggtcttag agcagagtaa gcgagcgtaa gcaaacttac tacgggagtg
     5821 cccgttacag cgcgacagta tacggaaaga agctaagtcc aagtactgga agaggctgtt
     5881 attgcgaagt aacggtaaat caaggtggta acacggaagt tcaagctttc gtccttttgc
     5941 ttaaggcaag agggcgaagg cttttttgtt tgaaaaatca taagagaaag atgtgaatgg
     6001 catgtcatta atccaactaa cgaatgtcaa aaagacattc aatgggaaaa acggaaacat
     6061 ccaagccgtc aatgacgttt cactaaacgt tgaaaaaggg gatatttatg gaatcgtcgg
     6121 ctattctggc gcaggaaaaa gtacattagt ccgtctcctg aatggacttg agcttcccac
     6181 gagcggcgaa gtgatcgtaa acgatcaaga tattacaaaa ttaaaaaaca aagaattacg
     6241 tacattccga aagaaaatcg gtatgatctt ccagcatttc aacctgttat ggtcaagaac
     6301 tgtacttgaa aatatccagc tgccgctgga attagctggc gtaccaaaga gcaaacgtaa
     6361 agaacgggca gaagaactgt tgcgtttagt cgggttagaa ggacgcggac aagcatatcc
     6421 ttcacaactt tccggcggac agaaacaacg cgtcggtatc gcacgagcat tggcaaatga
     6481 tcctgagatc ctgctttgtg atgaggctac tagcgcattg gacccgcaaa caacagaaga
     6541 agtattagac ttattgttag cgatcaataa gaaattgaat ttgaccatcg tcttgatcac
     6601 acatgaaatg aatgtgatcc gcaaaatctg caacaaagtc gctgtgatgg agttggggaa
     6661 agtcgtagaa gaaggagacg tattgactgt cttcagacaa ccgaaaaaag aagtaaccaa
     6721 acgctttgta caacaagaca tcgaaccaga agctgattcg acagaactat tagctgaact
     6781 catcaaagaa aatccaagcg gccgtttggc aactttgacc tttagcgaaa gaaatgccaa
     6841 cgagccagtc atttcccaag cgattcgacg ctataacatc gatgtcaacg ttgtgtatgg
     6901 aaagatcaaa caagtgaaag aaggttcctt tggttcattg actgtcttga tgactggtga
     6961 aaccaaagaa ttagatcaag cagaagtgtt catcaaagaa caaggagtag gaattgaggt
     7021 gatccatcgt ggataaaagc ttttcagaaa catatctaga ttttaaacaa atcaacccgg
     7081 aaacgatgaa tcaggctatc cttgatacac tatatatgac attaatctca atggtcttag
     7141 tgacgatcat cggattagtt ttgggattga tcctttactc tattggacgc aaaaacaaac
     7201 cgtatgctcg aatcatttat ggaatcgtat cgatcatcag taatattttc cggtcaacgc
     7261 catttatgat cctgatggtt ttgatcattc catttactaa agcaatcgta ggcactatgc
     7321 taggtgcgca agcagccatt ccagctcttg ttttatccgc agcaccattt tatgcccgtc
     7381 tagtagagat tgcttttcgt gaagtcagcc ctggtgtact ggaagcagca gatgcaatgg
     7441 gagcaagtta ctgggaaatc atctggaagg tcttgatacc tgaaagtatt cccgcattga
     7501 tttctggatt aacagttaca acgatctcga tgatcggatt tactgcgatg gccggcgcga
     7561 tcggcgcagg tggacttgga ggtcttgctt ggcaggaagg ctaccagcgt ggtaatctaa
     7621 cagtgacatt cgttgcgact ttaattattt tactaatcgt atttatcgtt caaggaatcg
     7681 gtgatttctt aactaagaaa acagacaaaa gataatagga tataggggga tgtaggatat
     7741 gaaaaagaaa atttttggat ttgcagcagc gcttttgctg actgtaggat tagcagcatg
     7801 cggtaacaac agcgacagca aagattcagc aaataaagca gacgacacga cattgaaagt
     7861 cggcgcttca ccgacaccgc acgcagagat tttggaacat gtgaaaccat tattgaaaga
     7921 tgaaggaatc gatcttgaaa tcgtcaaatt cgatgactat gtattaccga accaatcttt
     7981 ggaagaaggc gacattgatg ccaattactt ccagcatatt ccttatttga acaaagaaat
     8041 caaagaaaaa ggatatgact ttgtcaatgc cggtgctgtc cacatcgaac caatgggctt
     8101 gtactctaaa aaagtcaaag atgtttccga attaaaagat ggtgcaacaa tcattacttc
     8161 aaattctgaa tcagactggg gacgcatcat cacaattcta caagatgctg gcttagtcaa
     8221 agtcaaagat ggagtagatc tggaaacagc aacattcgat gatattgaag aaaaccctaa
     8281 gaacttgaag ttcaaccata cgatcgaccc atcactttta gcttctacgt accaaaatga
     8341 tgaaggtgac ctagttgcaa tcaacgcaaa cttcgcttac ggcgcagggt tgaatccatt
     8401 agaagatgcc gtattgcttg aaaaggacaa ctctccttac gtgaatatta ttgcgactcg
     8461 taaaggtgat gaagacagtg acaaaatcaa aaaactgatt gaagtgcttc accgcaaaga
     8521 agtccaagaa tggatcacag acaaatgggg cggctctgtt aaaccagtaa aggctgatgc
     8581 aaaataaggt atggaacaaa aaagatgaga aataagtctg taatggctta tttctcatct
     8641 tttttatacc tattctaatt tagaaatcaa aattgtttaa taataaataa aagatatttg
     8701 attttagtaa ggaaggtcat tgcgcttcat tctcaattag tctaaaatga gtgagaacga
     8761 aagagaattt gggggtataa tgcatgtctg tcttagaaat aaaaaatcta catgtgtcga
     8821 tcgaggaaaa agaaatcctt aaaggcgtaa acctaacaat gaaaaccgga gaaatccatg
     8881 ccatcatggg acctaacggc acaggaaaat ctaccttatc tgccgctatc atgggcaatc
     8941 caaactatga agtaacagaa ggcgaaattt tgtttgacgg gaaaaatgtt ttagacttgg
     9001 aagtagatga acgtgcgcga cttggcttat ttttagctat gcaatatcca agtgagatac
     9061 caggtatcac gaatgcggaa ttcatgcgtg cagcgatcaa tgcgaaacgt gatgaaaacg
     9121 acaaaatttc tgttatggca tttttgaaaa agttagatga aaaaatggct ttactaaata
     9181 tgccagaaga aatggctgaa cgttatctga acgaaggttt ttctggcgga gaaaagaaac
     9241 ggaatgagat cctgcaattg ttgatgttag aacctacatt tgcaatttta gacgaaatcg
     9301 attcaggtct ggatatcgat gctttgaaag tcgttgctaa aggagtaaat gaaatgcgcg
     9361 gagacaattt cggtgcattg atcatcaccc actatcaacg attattgaac tatatcacac
     9421 ctgatgtcgt tcacatcatg atggaaggcc gagtagtcat gacagggaat gctgacctag
     9481 ctaaacgtct ggaagcagaa ggctacgctg gtatcagtaa agaattagga atcgactata
     9541 aagaagaaga agcgtaaggg ggagcaagaa tgaaagaaaa aaactggctc gattatcttg
     9601 acgcggtaaa tgatttttct ttaagtaaag gcgaacctga ttggatgaga accttccgtc
     9661 aagacgcact agcgaaagca gatgaactgc cattgccgca tatcgatcgg gtgaaattcc
     9721 atcgctggtc gttatttgat gtgaaagaaa cacaaaccat cagtgaaaca ggcacgattc
     9781 cggcatttga tgcaatgaaa gacaatcctg tgcttgtcca acaaggttca tggacgattt
     9841 ttgaacaact gcctgtggaa ttagcagaaa aaggagtcat ttttaccgac ttgttcactg
     9901 caatgatcga atatccagaa ctagtccaag aatattatat gaaaaaagca gtaaacatga
     9961 acgaagacca attgactgca ctccatgtag cttttatgaa cagcggtatt ttcctgtatg
    10021 tgcctaaaaa tgtcgtgatc gatgaaccac tcgagtcctt attcattcaa gatggcgcat
    10081 ccgatgagca cttcttcaag catgtgttga tcgtggcaga tgaacatagt gaattttctt
    10141 atctggaacg tttccagaca accaaagaac aagtagcaaa atcatcaggt aatatcatcg
    10201 tggaagtcat cgctaaagct ggttcaaaaa tcaaatattc agccgtagat caattaggag
    10261 aaaatattac ttcttatatg aatcgccgtg ggcacatttt acgagatgct tctgtcgact
    10321 gggcaattgg tgtcatgaat gacggtcatg tgatcgctga ctttgactca gatttagcag
    10381 gagaaggtgc acacgcagaa gtcaaaatcg tggcaatcag ctcgggcaga caaatccaag
    10441 gaatcgatac acgagtaacc aataaagccc ctcatacgat cgggcatatt ttgcaacacg
    10501 gagtgatccg agaaaaagga acattgacct ttaatggtat cggccacatc ttgaaagggg
    10561 caaaaggtgc agatgcccaa caagaaagtc gtgtcttgat gctttctgat aaagcacgtg
    10621 gtgatgcgaa tcctattctt ttgatcgatg aaaacgaagt aactgctgga catgctgcca
    10681 gtgtaggacg agttgatcca gaagaaatgt actatctgat gagtagagga ttgcataaag
    10741 aagaagcaga gcgcttagtg attcgaggat tcctcggttc tgtcttaaca gcaatccctg
    10801 tagaacaagt tcggaaagaa ttagtcgaag taatcgaagg gaagttgaat ggatgatcga
    10861 tgctaaaaag ctacgcaatg attttcctat cttatttcaa gacgtcaatg atgaaccttt
    10921 agtttatctg gataatgcag cgacgaccca aaagccaaaa caagtactcc aggcattagc
    10981 gtactattac gaacacgata acgccaatgt tcatcgcggt gtccatacgt tagcagaacg
    11041 cgcgacccat gattatgaac aagcaagaga aaaagtacgg gcgttcatcc atgcaaaaga
    11101 aacagcagaa gtgctgttta ctcgtggtac gacgacaagc ttgaattggg tagccagaac
    11161 cttcggcgac cgctatatca aagcaggaga cgagatagtg ctctcttaca tggaacatca
    11221 ttctaatgtt atcccgtggc agcagttggc aaaacgaaca ggtgctgtct tgaaatatat
    11281 cgaactaaca gaagaaggct tcctggatat ggaggatgca gcgaaaaaga ttacggacaa
    11341 aaccaaaatc gtctccattg ctcatgtatc caatgtctta ggtgtcttca atcctgttca
    11401 agaattagct gaacttgtcc atgaaaacgg cggggtcatc gtagtagatg gtgctcaatc
    11461 tgccccgcac atggccattg atgtccaagc actagactgt gatttctttg ctttcagcgg
    11521 acacaaaatg tgcggaccaa caggtattgg tgttttatat ggtaaacgaa aatggttaga
    11581 agaaatggaa cctgttgaat ttggtggcga aatgatcgat tttgtcgaat tatacgacag
    11641 tacatggaaa gaactacctt ggaaattcga agcagggaca ccaaatatcg ccggcgctat
    11701 cgcattaggc catgcaatcg attatttgca aaaactcggt atggacaata tccatcgata
    11761 tgaagaagaa ctagccgctt atgtgttgcc aaaattacaa gcaattgatg gattgactgt
    11821 ttatggtcca caagacccga attttcggac aggagtcgtt gcattcaatt tggatgggtt
    11881 acatccccat gatgtcgcga cagctttaga catggaaggg gtagccgtca gagcaggtca
    11941 tcattgtgcg caaccgctat tgaagtacct tgaagtatca gcgactgctc gcgcgagctt
    12001 ttatttctac aacacaacgc aagacgccga ccgtttagtt gaagcaatca tggcaacaaa
    12061 ggagtttttc caacatggga ctatctaaat tagacaattt ataccgtcaa gtgatcttgg
    12121 atcattcttc ccatccgcat catcgtggaa gcttgactga ctcgacaagc aagatcgaat
    12181 tgaacaaccc cacatgtgga gatgtgatcg aattgcaagt cgatgtaaaa gacggaatca
    12241 tcaaaaatat cgcctttgac ggcagtggct gctccatcag tacggcaagt gcctcgatga
    12301 tgacagacgc cgtcatcgga aaatcaatag aagaagcaga acatttaact gtagactttc
    12361 tacagcttgt ccaaggaaaa gaagtcaaag atcccgacca attaggcgat gctgctatgc
    12421 ttggtggtgt agctaaattt cccgctcgga tcaaatgtgc gacacttgcc tggaaagcac
    12481 taggaaaagc aatggaaaac gatggtaccg cagctttcca tcattaccac aaggaagagg
    12541 agtgagaaca tgggcgtacc agaattagaa gaatataaat tcgggtttca cgatgatgtc
    12601 gaaccggttt tcagtacagg agaaggcttg acagaagaag tgatccgtga gatgtcaaga
    12661 atcaaaggcg aaccagaatg gatgcttgaa ttccgtctga aatctctaga aacatttaac
    12721 aaaatgccaa tgcaaacatg gggccccgac ctttcagaca tcgatttcga tgccatcaaa
    12781 tattaccaaa aaccaagtga caaacccgct cgtgactggg aagacgtacc agaaaaaatc
    12841 aaagatacat ttgaaaagat tggtatacca gaagctgaac gagcttatct agccggtgct
    12901 tccgcacaat atgaatcaga agtcgtgtac cataatatga aagatgaatt cgaaaagtta
    12961 ggaatcgtct ttactgacac tgactctgct ttgaaagaat accctgaatt attcaaagag
    13021 tacttttcaa aacttgtacc gccaaccgac aacaaattag cagcgctgaa ttcagccgta
    13081 tggtcaggtg ggacctttat ttacgtgcca aaaggcgtca aagtcgatgt tccattacaa
    13141 acgtacttcc ggatcaatgc ggaaaacacg gggcaattcg aacgtacgct gatcattgtt
    13201 gacgaaggag caagcatcca ctatgtcgaa ggttgtacgg cacctactta ttcaagtaac
    13261 agcctgcatg ccgccatcgt agaaatcttt acaagaaaag atgcgtattg ccgttacaca
    13321 acgatccaaa actggtcaga caatgtgtac aacttagtaa ccaaacgagc aaaagcatac
    13381 gaaggcgcaa ccgttgaatg gatcgacgga aacctaggtg caaaaacaac gatgaaatac
    13441 ccaagtgttt acttagatgg aaaaggtgca cgaggcacca tgctctccat cgcctttgca
    13501 ggagcaaacc aaatccaaga tacgggtgca aaaatgatcc acaatgcacc aaatacatct
    13561 agctctatcg tctctaaatc aatttcaaaa gacggcggag aagtaaacta tcgaggacaa
    13621 gtaacctttg ccaaaaacag tgcgggttcg atctctcata tcgaatgtga cacgatcatc
    13681 atggacgaat ggtcgaaatc cgatacgatc ccattcaatg agatccacaa cagccaagtc
    13741 tctttagaac acgaagccaa agtctcaaaa atctcagaag aacaactcta ctatctgatg
    13801 agccgaggcc tgaccgaatc tgaagcaact gaaatgatcg tcatgggctt tgttgaacca
    13861 ttcaccaaag aacttccaat ggaatatgca gtcgaactca atcgattgat cagttatgag
    13921 atggaaggca gtgttgggta aacctgttat aggagctaga acccttgata tataagggtt
    13981 ctagcttttt tgttttgcct gtatactaga aatgaccaga ttttgaccag ctcttttact
    14041 gatgagccat catatcagca aagtcttgac ctgttttctt aggtgcatta tttgtaatat
    14101 gtgcatagat gtctaatgtc actttactat tttcatgccc taatcgttgt tgaactacct
    14161 tgacagattc tcctgcttct aataataaac tagcatgtgt atgtctaaac ccatgaggtg
    14221 taatacgagg caagttatat tttttcaaaa tatatcttaa ccaatcatta acagtgtttg
    14281 gaatgtatag agtgttttta actgttgtaa aaacatgttg ctctttagag ctagtattgt
    14341 atccaagttt taaatactct tctttttgct gtaatttcca gttgttgagg atagaaagag
    14401 tctcagtatc taaagagata actcgtctag aagctcttgt tttcggagat tgaattataa
    14461 ctttaccaaa ttcatctaca gcaagagttt tattgacatg tagttcttta ttaaacgtgt
    14521 ctatgtcttt ccactgtaaa gcgagtactt cactctttct catacctgtg tatgccaata
    14581 atctgaagaa ggcgtattgt ttcatgttac caaaatcttt aaaagcatcc agtaaattat
    14641 taagctcctc ttttgagtaa aatttacgat ttttctcata ctcttttttt cgtggaagaa
    14701 gtgttttttc cataggatta ttgtcaatat attgcattgc aaccccgtag cgtaaaattt
    14761 gtgcagtctg ttttctcata taatcgtaac tttcatattt actgtgccat tcatctacca
    14821 ctttttgaca atatgggaca gagatattcg atagtttaag cttaccaaat gctggaagta
    14881 tttgattatt tgcgtatctt actgagattg cgattgttga agctttaaca ctggtacgct
    14941 gatgagttat ccattcatta tacaggtctt caaatgtcat atcaggtgct ttctttttaa
    15001 caccgtataa tatttgatgt ctgtactcgt cataatcttt ttgagcttct tgtttgctac
    15061 taaaacctct tctcgtagta tttatttttt caccagttcg tggatcggtg gcaatgtgtc
    15121 ctttatacat ccacgccact tgaccgtttt tctttttgta ctttgatatt tttgccatta
    15181 aatcatctcc tcttagttta caaggagtac ttaccactag atggtatcac ctcccttcat
    15241 ttttttctta gtgtattttt tcccaattga tgaaatcgtt tgttctagct tgtttatatt
    15301 atcagtaatt tctctcacag cttcttcggg ataagacatg tctttcaatt gaataggtag
    15361 ccattcatct accattcttt ctagtagttc ttctatactg ctagtgagga aaagaatgtt
    15421 attgtcaaat tcttctacaa tagcattgat gaataattct tctattttta agatattgaa
    15481 aagactaatt ttttcatttt taactttatt acaagtcttg ttagcaataa attcttgcat
    15541 ctctggttct aatttggaat atctatccag ccatgtatct aaattagagt gtactggtgg
    15601 gaactgttga cgaagatgct ggaacaggtt tttatctgga aatgttatac catctagtac
    15661 aatgctatct ctataaagaa taattcccaa aatatattct tctacattgc cacaaactaa
    15721 ctcctctata ctcatatttc ccaaatcagc aattcgggat aatcgctctg gtattggtaa
    15781 ataggttccc ttctcccatt cgtagatagt ttttttgtca attaattgac cgttgttaat
    15841 cataagacca aatgctggtt gtgttaattt tttactcttt cttatagctt ttatttttgt
    15901 tccgagtatc ttttttcgtt ctaaataatt tattggtgga cgttttatca tattgaccac
    15961 acctcctaaa attagtatac ctcaaaaaag gggtgttgta aataaatttt acaaagctgt
    16021 tgacacccct tttttatcga ggtattatgt gtatgtcgat gaactggtaa tcattttttg
    16081 tttgattaat cggttaataa caaaatactt ttattttgag caaaaaatac gaagataatt
    16141 cttagaaaat ttgttcaagc aatcacgaaa ggagggggaa atatgtctct acaaatcatt
    16201 gatagcattg tttctataca agttgaacaa caagtgaaag agataattac agtgctaaag
    16261 cctatgctag aagacattat ttctggagtc actctagagt atatggacta tcagcaggca
    16321 agtgaatatc taggtgtatc agtaggaacg attagaaaat atgtttctca atacgggtta
    16381 cctgttataa aaattgatac ggtcattaga tttaaaaaat ctgatattga tagtttcatg
    16441 gaacggtata aaggttgagt aaacaaataa gactaaaaca tatagtaata aagagtttgt
    16501 ttttattatc tgtatgtttt agtctttaac agaattttat aaggaggaaa cacaaatgga
    16561 aacatcaatg attaatattc caatttatgt agatcaagaa aaattattag agaagccagt
    16621 tacaaaaaaa gaaaaaactg atgtaagaga taggtgttta gataactata gagaagttac
    16681 aagagatgag tttgtagaga tactaaattc atcacaatca tttattccaa gcaagacaaa
    16741 gaataaaggc aatgacaagg agagttttgt ggaaaccaga gtaattattc tggatgtcga
    16801 taacactgtt aaagatgaga aggggaaggt agtagatttg agtaaagatg atagtagata
    16861 cttaagcata gaaaaggttt tatctatcaa tttagttcat aattctgcgt ttgcgataca
    16921 aaaaagtatt cggtactctg aaaatctaga aaaatacaag attgtctttc tattaaaaga
    16981 agcaattact gactatggta aaatggttgc tgtttacgaa tacttgaaaa agaacatccc
    17041 cgggtgtgat gacaatgtaa atgcttcgaa tagaatgttt tttggaggat ataagtcaga
    17101 tgcagtgata ataattaatg aagacaacat gttagatatt actgagcttc cgattgattt
    17161 taccatgaca tgttctaaaa attgtcaaag tgattcaggg agagagtatg aactagtcaa
    17221 tgaaaccgat tttgttaagt tgataaaaaa tgacgacaaa gaagaaatga aacagtggtt
    17281 taaagactgc ctgtttgatt cctccgacat gtcttttaat gagatttatg aaagattgtt
    17341 aaccattgat atgaataaat tacttaagtc aagtaaaaac ttaagatgtc tgtttcacaa
    17401 cgatcataat ccctctgcta gtatttttac cagtaaaact gggcattcca ttttttattg
    17461 tcattccagt aattgtgata tcagtgaaaa ttttattgga gttgtgatgc taattttaaa
    17521 gaaagacagt cgggtagaaa catttaattg gctattagag aacctagact tgtatcctac
    17581 acattttcga aaattgaaag aggaatcaaa agtacttttt gacactcttg aatcaatgaa
    17641 agacaaactt ttcaggacaa taaggaatta tctagaggaa atgcgacgtg tctatgatgt
    17701 cttgctttct gatgttaatt tttttgatga tagtaaggaa agtgtgactt gtattttgtc
    17761 gggggaacaa ttagctaaaa aaatgagttc ttactatggt aaaacttatg acatagataa
    17821 atgtaataag ttactatcac ttatgacttt tataggattg ataaaaaaat tagatgatga
    17881 agatgttcct ttgaagatgc tcgaatacct aaataggtta aaggaagaga aagcgttagg
    17941 aaactttaat cataagagaa gcaatgtcta tcgattagac gtttttaatg caacttctgt
    18001 gattcaaaaa ttgaatgaag atatccttcc acttctggaa aaaattcatt ttacatatca
    18061 atatttttct tatgaatggg ttaagatatg ttttaacgaa aaagaggcta aaagagtatt
    18121 tcctcaaaat tcccgatcag aactcagcaa ggaaaaacaa ttgattttag atgaagcaca
    18181 tgaagtttta cagggcttat cgtggcaagg gacatatcta atgacagaga aagaacttat
    18241 taacactgtt tgtgaacgcc tacgtaatgt tggggataac agcgtgaaaa atgctttaat
    18301 aaaaaacaga ggctactttg ttaaacaagg attcactctt gaacgagctt ctaaggatat
    18361 taagaaacta tacaatgtga aagcaagtag tagtttttta atatatttag cgaaaggggc
    18421 agggatagat gaaacaataa aagaattgat attatcagga gctgggatag ttcctctgta
    18481 tcaatttaca agtgtaacaa gagatgatgg acggagagta aggaagagaa aagacccaat
    18541 tgtattaaac tagacaggtt atccatagct cttaatctta tttcatctca atacacccac
    18601 cgttaaagtt cggtgggtta tttatttttc aatctttcaa tagctgattg cgttcatttt
    18661 cttgcctttt taatagcttc atatagtcaa ttgttcgaac tgcatcttca gtgctaagag
    18721 aacgatagag agccaaaaac tcttcctcat aatctacgac aacattgtct agaaaatggc
    18781 ttacaggggt tcctagggct ctagaaagag ctgttaagct atcgaggtta ggctctgtta
    18841 cagaattttc ccatcttgct actgcctgtt gtgtagagaa gattttttca gctaattctt
    18901 tttgtgtcca cccgagagct tttcgctttt ctctaatgac ctttgcatat tgtttctgtt
    18961 tcattaatac acccccatct cagtatagta caacaagttg ttgtatttgt aatggtattt
    19021 gaagtttttt atattttatg agttgaaata catcatattg ttgtatataa taaaacagga
    19081 ggggagaatc atgaaaaaag taaataatct cagccgatac agaaaagaaa aaggactttc
    19141 tcaaacggag ttagcaaaaa aaatgaatgt tacacagcaa tgtgtaagtt cgtggcaaac
    19201 agggagaacg attcctaaac cctatcaaat gaagatgctt tctgagatat taagtgtacc
    19261 aatgaatgag ctgttttctg atgtatttaa taaagtaaac agttaactaa taagcaattg
    19321 aatgatttta ggaggagaag acatgtattg tagaaaatgt ggcaaagaaa taacagatga
    19381 aagtaagtat tgccagtttt gtggagcact acaagaaaca gatgagacaa ccggaacaga
    19441 cacagtaaag aaaagtactg agcagacaat taatgaaatg aaggaacaaa tcgtacatgg
    19501 acaagagaca gggaagctgt atttgattat tggttggatt tctatggtag tatcgttagc
    19561 atttattcct gttgtatttg gtgctgttgc tgttattatg ggctacttat atcgagaaaa
    19621 agatgagaaa atgggtacga ttttgatgat agcaggggta gctggagcaa tctttggtgt
    19681 acttttaggg atgtcaatgt actgataaga gggataagaa caaattattt tggagatagg
    19741 agataaaaag ttgaataaag taaatatgaa caagaagttt gtcattatag gtattgttgt
    19801 cttggctatt ataggtatta ttttttcaaa aatgacaggt acatctggat tagagaagtc
    19861 atcaaaacca ttggtaacac agattttaca agaacagtac aacttaaatc gatcatgtga
    19921 tgatgtgacg attactcatg aaaatggaga taacacatac cgggctaaag ctatattaga
    19981 caatggaagt gctatcaaca taaatattga gtactatcca aagaaagacc gtatatatgt
    20041 ggagatacca tatgctgagg tgttaatgtt aaattgattt aaatgtatta tgcataccgt
    20101 ccaaacagga actcccaaag gtgtaaattc actagggagt ttttatagtt tcctataaat
    20161 taaatactaa tacaggtgac aaaaaaatat tacaatgatt gtcgggaatt tttatgttat
    20221 tcactgttat aacaatataa aagaaatgaa aaagaaaaaa ttattatgga tatgtttggt
    20281 atttttattg ataattggtg gaattgaaat acttacta
//
LOCUS       DAXWOO010000039        20276 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.039, whole genome shotgun sequence.
ACCESSION   DAXWOO010000039 DAXWOO010000000
VERSION     DAXWOO010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.039"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(65..2140)
                     /gene="glyS"
                     /locus_tag="ACGE70_001116"
     CDS             complement(65..2140)
                     /gene="glyS"
                     /locus_tag="ACGE70_001116"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFF8692766.1"
                     /translation="MAKDLLLEIGLEEIPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRASRGHRFLGEDVTFTHAKQYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2142..3059)
                     /gene="glyQ"
                     /locus_tag="ACGE70_001117"
     CDS             complement(2142..3059)
                     /gene="glyQ"
                     /locus_tag="ACGE70_001117"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFF8692767.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            complement(3443..4255)
                     /gene="recO"
                     /locus_tag="ACGE70_001118"
     CDS             complement(3443..4255)
                     /gene="recO"
                     /locus_tag="ACGE70_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFF8692768.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(4398..5297)
                     /gene="era"
                     /locus_tag="ACGE70_001119"
     CDS             complement(4398..5297)
                     /gene="era"
                     /locus_tag="ACGE70_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFF8692769.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5312..5707)
                     /locus_tag="ACGE70_001120"
     CDS             complement(5312..5707)
                     /locus_tag="ACGE70_001120"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFF8692770.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5691..6167)
                     /gene="ybeY"
                     /locus_tag="ACGE70_001121"
     CDS             complement(5691..6167)
                     /gene="ybeY"
                     /locus_tag="ACGE70_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFF8692771.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6184..8376)
                     /locus_tag="ACGE70_001122"
     CDS             complement(6184..8376)
                     /locus_tag="ACGE70_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFF8692772.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8400..9371)
                     /locus_tag="ACGE70_001123"
     CDS             complement(8400..9371)
                     /locus_tag="ACGE70_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFF8692773.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10077..11456
                     /gene="brnQ"
                     /locus_tag="ACGE70_001124"
     CDS             10077..11456
                     /gene="brnQ"
                     /locus_tag="ACGE70_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFF8692774.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
     gene            complement(11610..12056)
                     /locus_tag="ACGE70_001125"
     CDS             complement(11610..12056)
                     /locus_tag="ACGE70_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFF8692775.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(12083..12259)
                     /gene="rpsU"
                     /locus_tag="ACGE70_001126"
     CDS             complement(12083..12259)
                     /gene="rpsU"
                     /locus_tag="ACGE70_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFF8692776.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(12421..12849)
                     /locus_tag="ACGE70_001127"
     CDS             complement(12421..12849)
                     /locus_tag="ACGE70_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFF8692777.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGKIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            12941..13801
                     /locus_tag="ACGE70_001128"
     CDS             12941..13801
                     /locus_tag="ACGE70_001128"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFF8692778.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            complement(13893..14243)
                     /locus_tag="ACGE70_001129"
     CDS             complement(13893..14243)
                     /locus_tag="ACGE70_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFF8692779.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(14258..15985)
                     /locus_tag="ACGE70_001130"
     CDS             complement(14258..15985)
                     /locus_tag="ACGE70_001130"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFF8692780.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            complement(16199..17125)
                     /gene="trxB"
                     /locus_tag="ACGE70_001131"
     CDS             complement(16199..17125)
                     /gene="trxB"
                     /locus_tag="ACGE70_001131"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFF8692781.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(17270..18703)
                     /locus_tag="ACGE70_001132"
     CDS             complement(17270..18703)
                     /locus_tag="ACGE70_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFF8692782.1"
                     /translation="MGFRKDFLWGGATAANQCEGAYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDVFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSNN"
     gene            complement(18703..20106)
                     /locus_tag="ACGE70_001133"
     CDS             complement(18703..20106)
                     /locus_tag="ACGE70_001133"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFF8692783.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLSADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
BASE COUNT         5497 a         4371 c         3601 g         6807 t
ORIGIN      
        1 aacaaataaa ccagagaaat agcctatttt gtgcctgttc tctggtttat ttacatatta
       61 gaaattattt tgtgatgatt tgatctaaac ttgctagtga caaagccatg gcagcaattt
      121 gacttaattg cgttaatcgg ttcttacgta ctgctggttc ttctgccatg accatcgtct
      181 gatcaaaata attttcgatc agtgggcgaa ggcttacaag ctgatcataa ttttcgttga
      241 tcgtttggtc agcaaagctt gttttcattg cctctactgc tttgtgcagt tctttctccg
      301 cttcgttttc gaacagttca gggtctactt ctgattgttt ttcaagctcc gcttttttcg
      361 ctaagttcac gacacgtgtc aaagcttcca tcgatggttt gaagtcttca tctgcaagat
      421 gctgcttgaa caattgtgct gctgcaaata cttttgtcag atctttttgt tctgcattca
      481 aaacagcttc gatgacatca tgacggatat tcttcgttgt caataattgg cgcagacggc
      541 ctttgataaa gtcgatcacc tgttgttgtc ccgctgacaa gccaatgcca aaacgatccg
      601 tatctttact gataacttca ctaatcgttt cttgcagaac agacaaagga aaggcccatt
      661 ttttcgattc gatgattcga acgacaccat atgcttgacg gcgcaaagca tatggatcgt
      721 ttgagccact tgggatcatc ccaactgcaa agaaagaaaa gatactatct aatttatcag
      781 caatcgctaa aacagaacct actgtagtct caggtaaatc tccttcactg gatataggca
      841 tgtaatgttc acggatcgct gttgctacgg ctggtttctc gcctttaagc agagcatatt
      901 tttcgcccat gatcccttgt agttcaggga attctcctac catgttggtt actagatcaa
      961 atttatagat ttcagcagcg cgttgcagat ccatcaattc ttcctcagat agtcctactt
     1021 gttcgcctat gatgcgagcg atgacttgta cccgttgcat tttctcatag atgctaccga
     1081 ttttttcatg gaaagtaaca gtttttaatt tttcaacgta ttggtcgatt gaagctttct
     1141 tgtcttcatt ataaaagaat tccgcatctt ctaaacgagc agtcaatact ttttgattgc
     1201 ctcgtaccac attttccaat tttacccgat cgccattacg agcagaaata aaatgaggaa
     1261 gcaacatacc ttgatcgttc cgtacttcga aataacgttg atgttctttc atagaagtga
     1321 ctagtacttc ttcaggaaca gatagatatt tttcttcgaa gtcgccagaa aagacagttg
     1381 gatattcgac taaattgttg acttcttcta gtaaatcttc atctagatca atgatccagt
     1441 gatttttttc tgctaattcg tttgcttgtg tgatgatcat ttcttttctt ttttgcggtg
     1501 aagcaataac aaattgttca ttcaattttt cttcgtattg ttttgcatgc gtaaatgtca
     1561 catcttcgcc taagaaacga tgacctcttg aagctctgtc tgttttgaca tccaagatct
     1621 caaacgggat tatttcgtca tctaacaatg ccacgatcca gtgaataggg cgaatatatt
     1681 caaaatcgta ttctgcccag tgcatcgtta ctgggaaagt gaggctttta atgacatctg
     1741 ctaaacctgt caatacttct tttgcttctt tgccgtgagt gtatttcgtt acatagacat
     1801 attccacgcc ttttaattct ctaaaagtga tagcatctgt cgtcagacct tgtccgcgga
     1861 caaatccttc agctgctttc gtccagtttc catctgcgtc caaagcaatt tttttggctg
     1921 gtcctttgac ttcttcttcg ctgtctgctt gacgttctgg aatgtttgtt actttgattg
     1981 ctaagcggcg cggtgtagaa aacgtttcga tcgtatcata agtcaattgg ttttcatcta
     2041 aaaattttgc tgttttttct tctaattgtt tcatgcttgg tgtgactaca tgagcaggta
     2101 tttcttccag cccgatttca agtaataagt cttttgccat cttattcagc ctccttcacg
     2161 ttttctggat ttgctgtgtt tttattcaat aaagggaatc ctaatttttc gcgttctgcc
     2221 acaaagattt tggctacagc acgagccatg ttgcggatac gggaaagata acctgcacgc
     2281 tctgttacgg atactgcacc gcgagcatct aataaattga atgtatggct acatttcaaa
     2341 atataatcat acgctggatg aacaagattt tcatcgatac agcgtttagc ttctttttca
     2401 aaacgatcaa aattgcttag tagcaggtct tggtcgctta cttcaaaaga atatttagaa
     2461 tgttcatatt ctggttgttt gaagatctca ccatatttca cgccatcagc ccattccaga
     2521 tcataaacac tttcaacttc ttggatataa gaagcaagac gttctagtcc ataagtgatc
     2581 tcagaagtaa ctggtttaca aggtagtcct ccgacttgct ggaagtatgt gaattgtgtg
     2641 atctccatcc catcaagcca tacttcccag cccacaccgg cacaacccat cgatgggttc
     2701 tcccagttgt cttcaacaaa gcgaatatcg tgttctaatg gatcaattcc taaaagacgc
     2761 aagctttcta aataaagctc ttggatattt tcaggtgaag gtttcattac gacttgaaat
     2821 tgatgatgct gatacaaacg gtttgggttt tctccataac gcccgtcagc tggtcggcgg
     2881 gatggctcta cataagcagc attccatggt tccggtccga ttgcccgtaa gaatgtatat
     2941 gggctcatcg tacctgctcc tttttctgta tcataagctt gcatcagcat gcagccgttt
     3001 gatgaccaaa atttttgtaa tgttaaaatc atatcttgga cggtcaattt tttactcatc
     3061 tgttttgccc ctttcaattt ttaagcgttg ctgtcaataa gaatacagga aagaaaacga
     3121 caaaaagtcc ctatgtctga ttacagacat agggacgaat aatttcgcgg ttccacccta
     3181 cttccgaata aagattcggc agcttcattt atcgtactcg caagtgcctc ttcaaaaatc
     3241 gtttgggtcc ggcttccact ctcccgaact cgctaaacca cgtgtttttt actgatcttg
     3301 gtcaatgtac gtattcattt tgcagttttc tggtttgtat aatagatcct ctcagtgaac
     3361 gatctattta atctacggtt aataatagac ggaagtccct gtatttgtca aatagttttg
     3421 atttttttgt tgtatctttc atttattctt ctttttcagg tatctctttt tcaggcatat
     3481 ttttctctgg cgtctttaat acgtgtgccc aagacttcat ttgatcaatg aactttttgc
     3541 tttttagatg aataccgaca tattcatcat acaactggtc aatgaggtca cggattgcct
     3601 gcttcgtttc ttctttcaag tggattccac tgatcttatc ataagaaata gccgaaaaca
     3661 ttcgaacgaa ataaacagcg cgaggatctg catgataacg gtgttcgtcc attggccagt
     3721 gtttctcgca tatcacaccg ccatatttgg aagaatagtc aaattttcct tttgtttccc
     3781 cgcatacgac acagtgcgtc caaattggcg cgattccgaa gcgttgcagt atttgcactt
     3841 caaagatatt cgtgatgatt tctgcatcat ctccttggtc catccgctgc agagcttgtt
     3901 gcgtaaattg gaataaatgc gggtcatatt gatgatcttc gattgctgca tctactagat
     3961 ttagcaaata cgtaccataa ccgctcaaaa agatatcttc ttggatgttc cgaaacgcat
     4021 ggacgtcttt gacactgctt aaaaacgaca aaccttcact tcgaaaatta ccgatataga
     4081 ctgcttcggt aaaggggagc aaagccggag ctaatggctg attttgccga tggattcctt
     4141 tgatataaaa catcattttg ccagaagatt ccgtaaagat tttgactaat ttatcttttt
     4201 ctttgtaatt acggctagat aaaatcaagc ctttcgattc tgcaagctgg ctcatattct
     4261 ccctcctaaa aaataaatct ggaaaagcag attctgccga tccagattat ttatgtttcg
     4321 atcttccgct gtttttctca acgtacggat ttttatccag tttatttcta tacctaacct
     4381 ataagaagtc cagaatatca gtactcgtct tttcggtagc cgaagtcttg cagatagact
     4441 tttttgtctc gccagtcttt ttggactttg acccaaagtt ctagaaatac tttgtctcct
     4501 aaaagatttt cgatatcctt tcttgctttc gttcctactt gcttcagcat ttttccgcct
     4561 ttgccgataa tgattccttt ttggctatct ctttcgacga tgatcgtggc ctggatatgg
     4621 attttatcat tctcgtttcg cttcatcgaa tcaacaacga ctgcgacaga atgaggcact
     4681 tcgtctcgtg ttaaaaacaa gactttctca cggatcaatt cagaaacgat gaagtattca
     4741 ggatgatcag tcacttgatc atctggaaaa tattgtgggc cctctggcat ttcactgact
     4801 aatacatcca ttaagcgttc gacattgttt ccttctgtag cagagatcgg aatcacttgt
     4861 gcaaaaggca tctgcgtcga ataatcttcg atgattccca gcaactcgtc tggatggatc
     4921 ttatcgattt tattgataat cagataaaca ggagaatgca cgttttttag acgttcaata
     4981 ataaaatcgt ctccccggcc acgtttttga tcagcactga tcataaataa gactgcatcc
     5041 acttctttca acgcactgta agctgcttct accataaaat ctcctagacg atgctttggt
     5101 ttatggattc ctggtgtatc gataaaaatc agctgagctt ctggtgtagt atatactcct
     5161 tggattttat ttctagttgt ctgtgccttg tcactcataa tggcaatttt ttgacccacg
     5221 attcggttca ataatgtgga ttttcctaca ttcgggcggc cgacgattgc gacaaaccct
     5281 gatttatgtt ctggcatata cgttctcctt tttaattaaa caacaaatga tatattttag
     5341 ggacaaataa aatcaaccca atgatgacag caaaacaagc ggtcactaaa actgctccag
     5401 ctgccatatc tttgattttt ttcccaatcg gatgaaaatg aaaatccgta aacatgtcca
     5461 ctacattttc aaaaacagta ttgatgatct ctactatcca aacgagaaaa acagctaaaa
     5521 gcagccataa ccactctatc cgatcaagct ggaaaatcac gcccataaat aaagcaagga
     5581 taccgaacgc aacatgtttc ctcatattgc gttcttcctc aaaaacggtt ttgactcctt
     5641 ggacagcaaa ttccacagac atcaggaaat gtttgttttt ctctacgttt ttatcttttg
     5701 agtccatagg catccaagat ctctttctgt aaaccaaaca tttctttctc atcttctggt
     5761 gtcatatggt catacccgtt gatatgcaag aatccatgta ccgctaaaaa gccaagttcc
     5821 cgatcatatg aatggccgta agcttctgct tgttcagcag cacgttcaat agagatcatg
     5881 atgtctccta gtacacgagg taatggattc tctccatctt caaaaatcac tgggatttcg
     5941 tcttcgcctt cttcttccag agcaaaactg atgacgtctg tcggtgcatc ttttccacgg
     6001 taatcacgat tgattacttg gattgccgca ttgtccatga aagtaacaga catttctgtt
     6061 tcctctggca gctttaaata agatgctgca aattgcagca agtcatcgat ttctttcatt
     6121 ttctcttctg agacttggtt tgtttcatcc ataaaggtaa tatccattat acatcgcttc
     6181 tttctattct tcttgttctt cctttatctt ctcaacttct gttttcattt taggatattt
     6241 gatccgagaa tggaaggtgc tacttaaacc gctgattaat gatttttcca cttttcggat
     6301 ttctttcaag gtcagtccac tatcgtctaa ttgaccatcg gaaatacgct cttcgataag
     6361 attatggaca aaactttcga ttttttcgct cgtcggatga tccattgccc gtactgcagc
     6421 ctcacaactg tcagcaatat tgacgatccc tgcttcacga ctttgcggtt ttggtccagg
     6481 ataacggaat tgctcttctg ttacttccgg attgcgttct ttcgctttga tgtagaaata
     6541 acgcattagc gtggttccat gatgctgttt gcaaatatcg atcaccattt gcggcatatt
     6601 ataatcttcc aatattttgg ctccatctgt cacatgtcca aagataatat gtttactgtc
     6661 ttctggcaat aagaaattgt gcgggttttc cgctcctgaa ggcaagtttt caacgaaaaa
     6721 gctagcatgt ttgatcttgc cgatatcatg ataatagcaa gctactcgag ttagcaaaga
     6781 acgtccgccg atttctgcta cagcgttagc acttagatta gctaccatca tactatgatg
     6841 ataagtacct ggcgcttctt ccagcaactg tttcaataaa ggatgatttg gattgctgag
     6901 ttcatttaat acaattacac tgtcatcatt gaccatcaat tcaatatatg ggtgtagacc
     6961 gatcgtcagc aagaatgaga agagtgcacc agtgaacccg cagacgagca ttaaccaagt
     7021 cgttgcatca ccaaagctca ttccttgata gacgatcaaa atgacatcca tcatcacagg
     7081 aaaaataatg acccacatgg cagcagataa tccttgttct gagatccgtt tgcgtctgac
     7141 aatcgttgcc atgagcccag agaaaacata agacatcaag ataatcgtta gaaagttcgt
     7201 gccaatcgaa tcataaaaaa tgaaaagtgc ggatactgct tggaaaatac cagccatgat
     7261 ccctgctctt cgattcaaga aaaagcttaa aacaagtggt gcaaaagcag cgggataaaa
     7321 cagcgggata taagtagctt gttccgtttg gaataattgg aaaaatttca tcagcactat
     7381 gctgactgac atagctgtca catagaaaag cacgtatttt gttctttctc ctgcattttg
     7441 gaattgaagt gaattataaa tcagtacgcc gatttgaagc aaaacagcta aaaccatggc
     7501 aattagcgga aaaatcgaag ttgtttgatt agtcagtcct agtaattcca attttttcat
     7561 agctgaagca tcgatttgat taccttcacg aacaatgatt tctccttgga agatcatcac
     7621 tggttgtaca gcttctcgtg cggattgttt caattcttct gtctttttct cattcaatac
     7681 gtcattcact acgatacctt gatccactaa gtagcgaatc atttgttgtt gtgctggtgt
     7741 gacattcaaa tattggattt gctcttctgc ttcttgttta aaagcttcca attcactttc
     7801 acgaacttgt ttgctcatct gttcgtcaat gagcttcaga ctctcatccc tcacagtatg
     7861 aatctcagtt tcagtcattt caaatatcgt cgaataaaaa ttgttcggta gtttttgata
     7921 aaacacaaca ttttctgcat tcacattttc aaaatttttc ttcagcgcgg cgatccgctc
     7981 ttctgtagta ggctttggga tattttcatc ttcctttgcc ttatcaatct tttcgtcata
     8041 agatttgttg atggcatcat tcgtcttatc gatcaattca aatagttgct tgatccgatt
     8101 gttctggtca tctgcaagat ctttttggta agtgtattct ggagtgactg cttctgctgc
     8161 cagttttctt ttttgttcgg tttcttccgt gttctcgatc gttttgttcg cacggatact
     8221 ttcttctgct acttggcctt ctctgaaatc attggccttt tgtttgacac tactaaatgt
     8281 aacgccgcag ataatgataa aaaacaagaa cagcaggaaa ggcaatgctg cctttccaag
     8341 tttttcttgt agtttctgca gtgttttatt caacatgtgc ttatcctcct aagcaaagct
     8401 tattcttgat ggagatctgc cttttcataa gctttgatga tttcggcaac tacagggtgc
     8461 cgaacaacgt cacttgcttc aaaattaaca aaatcgattt tcttgatttg ctttaatgtt
     8521 cgttctgcat gaacaagacc acttgtcgtc cctcgaggca agtctatctg acttgtatcc
     8581 ccattgacaa tcatttttga ttgataaccg agacgggtca agaacatttt catctgagcg
     8641 actgtcgtgt tttgtgcttc gtccaagata acgaatgcat catccaatgt tcgcccccgc
     8701 atgtaagcta aaggtgcgat ttcgattacg cctcgttcca tcaatcggtt cgtatgatcc
     8761 aatccaaaaa tttggtataa agcatcataa acaggccgca gataaggatc cactttttct
     8821 tttagatccc cgggaagaaa gcctagattt tcacctgctt ccacagcagg ccgcgtcaaa
     8881 atgatttttt gcacttcacc ttttttcaag gcagcgattg ctaatactac tgcaagaaaa
     8941 gtttttcctg taccggctgg tcctacgcca aatacgatat catgtttggc taccgattcg
     9001 acatatttct tttggccgct gttttttacc cgtataggtt ttccattacg gtctttgacg
     9061 atctcttcct catacatctc aacaaaataa tctaatgtac ctttttgccc cattttcaat
     9121 gccgtcacaa catcgggcgt acttacgtgg attcctcgtt tgatcaattc ctgtaatgtc
     9181 cgaatgactg aggcagccaa ggaaatctgg gattgctcac caatgatctg aatgatttct
     9241 ccacgtgtat ggatggttgt ttccgttgtt tcctcaatca gttttatatg cttgtcgtgt
     9301 gcacctaaaa gcatgctgac atcatcagca gccgtcaatc taatctctaa agaactagac
     9361 tcttccgtca aaaatagaca tccccttttt ttcgattcat tctttcctat tttaccatac
     9421 tcagaagaaa ctatatagca aataagaaaa ggttctaaaa gagttgctta accataagta
     9481 ccaagaaacg ttaaaaaact agccgcttcc attctctaaa ggtctcgttt gaataaaaaa
     9541 agctgatcat gtaagctatg cataagatag aggacaagta ttccgtctaa cttagcaagt
     9601 attctctggg tatttttctt gtttcaaaaa aagaaaacaa aatatttgaa atattcttgc
     9661 atttcaaaaa aaacaggtgt attataacgt caacaaataa atatacagca aagcgtcgag
     9721 gaaaacagta ggctattcag tgatttattc ccagtgagtc atgtaagtga gaaatgacaa
     9781 taaatcgaat agacgaacat catttccgag tcaatgtcga aaacgtagat cattgtggct
     9841 gcctccatta tcaggctagc ggataacttt atcgaaaagc gtccgtgaat gaagaagctt
     9901 ttttaagcga aaaaaggtgg taccgttcta gaatctagag cccttttccc aagtggaaaa
     9961 gggctttttt tatattttaa ggtggtggtt ttcatggaat ctgatattag tggtatttct
    10021 agtttctgca acgtttattt tgttactcaa aatgataatt attggaggaa aaatttatgg
    10081 aagaaacaaa aacttattca tggaagcaat tattgatggt cagttcactt atcttcggga
    10141 tgttcttcgg gtcaggtaat ctgatctttc ctgtacactt aggtcaaatg gctggatcaa
    10201 actggatcag cgctggtgta ggatttgcca tctcaggcgc tcttttccca ttattagcta
    10261 tcttagctgt agtagtgaca aaaagtgatg gtctgtatga cttagctaaa cctgtcggaa
    10321 aatggtatgc tgccttgttc ttgatccttg ttcatttaac gatcggacca ttctttggta
    10381 caccgcggac agcagcaact gcttttgaaa tggctgctaa accctttttg cctgaaaaat
    10441 atgattcatt aggtatgttt ttattctctg ctcttttctt tttacttgct tatttcctta
    10501 ctgtacatcc aacacgtttg gtcaaatatg tcggaaagta tttaaactcc ttgtttcttg
    10561 cattattagc tatcattttt gtcgtttctt tattccaacc catggggaat ctaaaccaat
    10621 ctgctgcaga ttcttatcag acgaacgctt tgacaaatgg cgttttagaa ggatacaaca
    10681 cggttgatgc agtagcttta cttgctttaa gtgtcacttt tgtccatgca gttcgtgatc
    10741 ttggttttaa agataaaaaa gtcacagaat taacagcaaa agctggtagc ctgagcgttg
    10801 cgttagaagt catgatttat tttggtctgg tccttctcgg tgcgatgagt ttgaataaga
    10861 tgtctttatc agaaaatggc ggaactgcac tttctcagat cactaatttt tatttaggta
    10921 acttcggtgc tagcttctta ggggtaatgg tcacattagg tgtcttcact actgctatgg
    10981 gactcgttgt ttcttttgca caagatttcc ataaactgtt ccctaaagtc agttatatga
    11041 catggctgcg gttaacgact tttgtctctt ttgtcgttgc gaatgccgga ttggataata
    11101 tcatccaatg gtctttacca gtattgatgc ttttgtatcc attgtcactt gcgttaatct
    11161 tactttcctt aacagcaaaa ttcttccaaa agagtccatt tgtctatcaa gtaacgatgt
    11221 tcttttctgc cgttccagcc gtattggata tgttggcaaa ttcgcctgca ttagtgagtc
    11281 agcaaactgt cgtagcaagc ttactaggat tttatcatca ttatgttcct tttgctgcat
    11341 taggtcttgg ctgggtcgtc ccaacgttgc ttggttacgc tggcagcttg ctcttttatt
    11401 atgtatatcg cgtatcagga tataaacaag aagcaagtga gttaccagaa gaataaccaa
    11461 atcaaacaca atttaacata agagatatta aacaggaaaa aaacagctag aagaaagaat
    11521 aagattcttt cttctagctg ttttttagtt ctttttttac gaacacaaaa aactgcggat
    11581 aatcactgtc cgcagtttaa atgataaaat taagattgta atagttcttt cacaatcgca
    11641 ttcacttggt ttccatctgc ttttcctttg actttaggca taattgcacc cataactttg
    11701 ccaaattctt tcattgatga agcaccagtt tgagtgattg cttcttgaac aagttgtcga
    11761 atttcttcgt cggaaagttg ttccggcata tatttttcga ctattacaat ttcacttttt
    11821 actttttcag ctaaatcgtc acgtcctgct tcttcaaact catgcaaaga atcacgtcgc
    11881 tgtttcattt cacgagacaa caccgttagt tcttcttctt ctgtcaagtc gtgaccttct
    11941 ttaatctgct cgttttgtat agatgacttg atcattcgga tgacttgtaa agattcctta
    12001 tctttcgctt tcatcgctgc tttcatgtca tcgtttaatc tacttagtag tgacacacta
    12061 ccaactccaa ttcatcacag aactagaatt ttttgcgttt tctagctgct tcagattttt
    12121 tcttgcgtct tacacttggt ttttcgtaga attcacgttt acgagattct tgtaaagtac
    12181 ccgcttttga cacggaacgt ttgaagcgac gaagagcatc gtcaagtgat tcgtttttgc
    12241 gaacgactgt ttttgacatg ttaattccct ccctccgagc ttaaaaagta accgtaattg
    12301 ttattgaaat atatctcaaa aaacaagact gtcctgtaaa tattatatct aatgctgttt
    12361 tgaccgtcaa ggaaatcttg ctttttttta aaattatcaa atgttatttt atttaaatcc
    12421 ttattgacaa ttttcacatc ttccgaaaat ctcgaatcta tgcccttcga tcgtgcatcc
    12481 acctagctgt tcttcgaaat aattcatcgg acacatatgg atttctttcg ttgttccgca
    12541 cactgtacag ataaaatgat ggtgatggcc gattttcccc tgacagcaat gaaaacgaaa
    12601 cttcatttct ccatttaaat cggtttcttc cagtaaatcg atctctgcaa aatcatggag
    12661 attccggtaa atcgtatcat aacttacccc tttgaattcc tgattcatat agtcaaaaac
    12721 ttctttagca gatacgtagc ggttacgctt cactaaataa gttaacaatg cttctctttt
    12781 tttagtatat ttaaaaccgt tatttttcaa tgtttctaat gattgttcga tcaatgtctg
    12841 ttttttcatg acttattcct ccaaatcaaa atgattacga agtacgcatt ctagagtatg
    12901 gtataataaa cataaaaaaa atgcaagtga ggttttatcc atgtcttccg attctacaga
    12961 aatcgttact ttttttgtca tctccgactc tgctggagaa actgcgacaa aactagcgca
    13021 agctactatg gctcagtatc ctaccgttga gttcaaccta ttccgcagaa cgttcgtgac
    13081 agatgaaaaa acattaaggc aggcattgca agatgcatta gaagaaaaag cacttgtcct
    13141 gcatacatta atcaatcaag aactgatcga gataacacgt tctttttgtg aagaacatca
    13201 attatttagt tttgatgtga tgacgccacc gatatcagaa attgaacgtt taactggtat
    13261 caagccaatt cggcaacctg gtgccctcca tttattgaat gagaactatt tcaaacggat
    13321 caaagcaatg gaatttgctg taaaatacga tgatgggaaa gatcctcgtg gttttttaga
    13381 agcagatgtc gttttgctag gcgtatcccg aacttccaaa acgcctttga gcttgttttt
    13441 agctaacaaa aatttaaaag ttgccaattt acctttgatc ccacaagcac atattcccaa
    13501 acagctttgg gagatcgatc ctaaaaaaat cgtgggacta acgaataacc cagatatttt
    13561 aaataatatc cgtaaagaac ggatgcgctc ttacggatta aaccctgata ctgcttattc
    13621 cgatatcgaa aaaattagag ctgaacttga ttttgcgaat gatctttatg aaaaactagg
    13681 atgtgtagtg atcaatgtgg catctttatc tattgaagaa acggcttcgc ttattttgaa
    13741 tgctttagac ctagaagatc atagttatta tggaactgaa acaacagaaa aaagcgatta
    13801 atcatttttg tctttttctt aaaaaacact ggagtgtgac aacgaagaaa tttcgttgtc
    13861 acactccagt gtttatctat ttgcacctat cgttattgat gttccacatg tttgagtgtc
    13921 tggttcaaaa tatccagtac atgagaatca tcaagactat acaaaatcgc ttttccctcg
    13981 cgatgagact tgacgacatg attttctctt aataaacgta gttgatgcga tacagcagac
    14041 tgctccattt ccactgcttg ggtgattgct gtcacatttt gttccccgtc tttcagatat
    14101 agcagaattt taagacgagt ggggtcactc aacattttga ataattgact gactttctct
    14161 acttttgatt tttgcgtgtc tggcaatcca ttcacctcca acttgaaaaa agactgactt
    14221 ctaaagttta ccactttttc catcagtctc aagtttttta ttcttctgtc atctttttga
    14281 taatcgcttc ataagaggct actttttcgg ctgcgtcttc tgtagagctt ccttttacac
    14341 caatataaaa cttgattttc ggctcagttc ctgaagggcg gatagcgatc cagccatttt
    14401 cttccaatgt atacttcaat acgtcggatg gcggtgttgt tagtttttct acgtttcctt
    14461 cacggtctgt tctcgtcaat tctttaaagt cttctgtttg aataactttt gctccggcaa
    14521 attctgtcgg tgcttcttta cggaattttt ccatcaaagc agagattttc gcagaacctt
    14581 cttgtccgct cattgtcaca gaaatcgttt tttctgcgaa atagccatag gtttcaaaaa
    14641 tcgtttgcaa gccgtcatac aacgttttgc cttgtttttt atagtaagct gctacttctg
    14701 ctaataaaag caatgcttgg atcgcgtctt tatcgcgaac aaaaggttta actaaataac
    14761 cataactttc ttcgaatcca aacataaaag tatgtgaatg gtcttcttca aattgttgga
    14821 ttttttcagc aatgaattta aaccctgtca acacattgaa catagtagca ccgtagtgtt
    14881 ccgcaattgc tgtaggcaat tcgctggaaa cgattgattt caataccgct gcgttttctg
    14941 gtaaagtgcc tcttgattga tgtgcttcca aaatatattg agcaaacaat gctccgattt
    15001 ggttcccagt caatacttca taagaaccat ctggcaaacg aaccgctgcg cctaaacggt
    15061 ctgcatcagg atctgttgca atcagcaagt cagctccctc tttttctcca agtcgtatag
    15121 catattcaaa agctgaatgt tcttctggat ttggcgattt gaccgtagtg aaatctggat
    15181 ctgccacagc ttgttcggga actagtacga attgttcgaa tccagcttgt ttcaacgcac
    15241 gttcgcccaa cattttccct gttccatgaa gtggcgtgta cacaagtttc aaatctttcc
    15301 ccattgtttc aaccaattca cgatcaatcg tcactgcttt gatttcttct agataagcag
    15361 cgtccacttc ttctccgatg atcgtgatca acccgctatg tttagcttct tcgtcagata
    15421 atacttcgac ttttagcgga ttgctgactt cacggacata tttcgtcaat gcatctgcgt
    15481 ctgcaggagg catttgtccg ccatcttctc cgtagacttt atatccattg taagccgctg
    15541 ggttgtgaga tgcggttacc atgattcccg caaacgtctt caaatgacga actgcaaaag
    15601 aaagctctgg tgttggacgc agactttcga atacataaga cgggatattg tgatgtgcca
    15661 gtgttttggc tgcttccatt gcaaattctg gtgactgatg gcgtgagtca taagcgatag
    15721 cgacacctcg gcgttttgtt tcttcacctt gtgtatccat gaaacgtgct agtccttctg
    15781 ttgcttgtct taccgtatag atgttcattc gattgatccc aggtcctaag attccccgca
    15841 tgcctgcagt accgaattcc aacggtgcat aaaaagcatc ttcaagtttt tcaggatctt
    15901 cacccagatc atttaattgt ttttttaagt tttcttctaa attttcttca tgtacccatt
    15961 gttcatacac ttgttcccaa gacatgaaaa taacacctct ttctgaaata tggttcttaa
    16021 taagtatagc attcacttat tagatatgaa agtatgagga ccaaaaaacg cttaaatcaa
    16081 gaaaggattc ttttatcaag catgattgca agtattcaca taaatcggat tcagtggatt
    16141 tcacgcgaaa aaaaagagta aaagaacaat tgaaaccgtt ctttcactct ttttaaactt
    16201 atgctgttgt ttcttctaaa gactccaagt atttatacac ttgttgtcca gcaataccgc
    16261 cgtcaccaac agctgtagtg atttgtcgca aatctttttc acgaacatct ccgatggcaa
    16321 aaacgcctgg catattcgtt ttcatttcgt tgtctgttgg aatccagcca gcttcgttcg
    16381 tcaaacctgc ttctctaaaa ggtgcagtta atggatccag tccaacatag atgaatactc
    16441 cgtctgctgc taaatcaaat tcattgccgg ttttgacatt acgtgctttc acacctgtca
    16501 ctaccatttc gtttccaacg atttcgtcaa cgactgtatc ccacataaag gaaatttttt
    16561 catttgcaaa tgcacggtct tggatgatct tttgtgcacg taattcatct ctacggtgga
    16621 cgatcactac ttcagaagca aattgtgtca aatagatggc ttcttctact gctgagtccc
    16681 cacccccgat gactactaga cgtttgttac ggaaaaacgc accatcacag actgcacagt
    16741 aagaaacgcc tcgtccagca aattcttcct cgccaggtac gcctaattta cggtggatac
    16801 agcctgttgc gataatgact gtttttcctt cgtaactttt atcttcacag atgacttctt
    16861 tatggctgcc gtgatccttg atatcttgaa cgattccata agcattttct gtcccaaatc
    16921 ttgagacact ttcatacatc ttataagcta gatcggggcc catgatcgaa tcgaatccag
    16981 gataattttc gatttctgcc gtattgttca tttggccgcc aggggcacct tgttcgatca
    17041 tcaatacgga taagtttgaa cgtgaagcat acaaagcagc agtcatccca gcaggacctg
    17101 cgccaataac gattacatca tacatatgta gattctcctt tacttaatca agtaagcata
    17161 ctttacagcc aatgcgaaaa aaagtctatt gttctgcttg cgtttctcga atagctttat
    17221 tatactcgaa aaatccgctg aaaaacaata gacttttata aatcattgat caattgttcg
    17281 aaagattttc tccatttgaa tcaattactt ttttatacca gttgaatgat tttttctttg
    17341 aacgggctaa tgtgccattt ccttggttat cacgatcgac ataaatgaat ccgtaccttt
    17401 tcttcatctc accggtacca gctgatacta aatcgataca tccccaagtc gtatagccta
    17461 atagatcgac tccgtctagt tctactgcat ccttcatcgc ctggatatgc gctgccaggt
    17521 aatcgattcg gtaatcatct tccacataac cattttcatc tggcgtatcg actgcaccta
    17581 agccgttttc tacaataaat aatggttttt gataacgatc ataaatatca ttcatcgtta
    17641 ttctcaagcc taatggatca atttgccacc cccattcact tgcttgcaga tatggatttt
    17701 tgacagaagc aaagatattt cctgcagtct gttccaagag ttcgttgtcg gtagtcgaaa
    17761 ctcgtgaaga ataataagaa aaagagataa aatcaacagt atgttctttc agcaatgcgg
    17821 catcccctgg ttccatttcg atctgaatgc catttcgctc catttctttc aatgcataag
    17881 cgggatattc tcctcgtgac tggacatcaa tgaagaaata attttctcga tctgattttc
    17941 tagaagccca aacatcttca ggattgcaag tgtatggata atatgctcct gctgccagca
    18001 tacagcctac tttgttctcc ggatctactt catgagcaat cttcgttgcg atggcacttg
    18061 caaccaactc atgatgtgcc gcttgatatt tgatttgttc ttcattttct ccttcttcaa
    18121 aatacagccc tgcgcccata aatggtgcat gaaggatcat attgatctca ttgaatgtca
    18181 gccaatattt gacaagtcct ttatagcgat tgaagatgac tcgacacaga ttttcataaa
    18241 aaccaacgag ttctctggaa cgccacgcac catattcttt gatcaaatgc attggacaat
    18301 caaaatgcgt aatcgtcaca agtggctcaa ttccgtgctt ttggcattct ttaaagacat
    18361 cctcataaaa tttcaagcca gcttcattcg gctctttctc atcccccatt ggaaaaatac
    18421 gactccaggc gatagacatt cgataagtct tgaaccccat ttctgcaaac aaagcaatat
    18481 cttctttata acgatgatac atatcgattg caatttttgc tggataaaaa tgatcctcat
    18541 caaattcaaa catttttttc tttccggtga taacaggaaa acggtctttt ccgattggca
    18601 caacatctac gtttgctagt ccgcgtccac cttcatcata agcgccctca cattgattgg
    18661 ctgctgtagc accgccccat aaaaaatctt ttctgaaccc cattatactt cctccattat
    18721 atgtgtatta cctgataatt ctcttgccag ttcatcttga acttcgattc cttcttcttt
    18781 taatcggctg attgtttcct tgaattgcgg taaatactgc tggtgtgcaa taaacaactc
    18841 atccatgatc cgttttgctg tctttcctga tcgtataagt ggattgatcg taaacgcctg
    18901 taaagctgtc ccataatcac cagtcacagc tgcttcgatc gtcagcaatt ccatattctt
    18961 cattacttgc aaccagcctc tttcagcagc aggaagagaa ccaaacgcta catttctcgc
    19021 tccttgtgcg ccaacatagg cagtgacttc tacaacacaa tctgccgaca ggtcaggaac
    19081 agctccatca ttctttgtag atacaacgat ctgtgtttct ttatttccat agattgaagc
    19141 aatcgtttca caggcagcgt ctgaataata agcgccacct cgtttttgca attgttcagg
    19201 tttatggtct aattctggat ctttgtataa ttcaaatagc tctgcttctg tttctttcac
    19261 ttgctgcgct cttgtaccaa tcgtttgata ttcttctaaa gcatgttcca gcatttcttc
    19321 ttcacgatag taatagcggt ggtagccaca agggatcatg ttcatttgtt ttaattgttc
    19381 tttgaaaaac gggatatcaa agatattttt cggtaatcca ttatcttcat cgtataaacg
    19441 atcaatcaat tctgccgtgc ggtcatttcc tttattatct gctactttgt gccaatggaa
    19501 atgatttaga ccggcaaact tgtagatcag ctcttctttt ttcttgccaa gcattgctgg
    19561 ttctgtcatc atcgccatca ctggcacatt gcataatcca atcactttat cccatttgcc
    19621 atatctgata actgcttctg tcaccatccc acttgggttc gcgaaattga tcagccaagc
    19681 atttggacat aaacgcttca tgtcctcaac gattgccaaa acgactggaa cggtacgaaa
    19741 agctttgaat atcccgccag caccatttgt ttcttgccca atcatcccat aataactagg
    19801 gatccgctcg tctttgattc gtgcatctaa ttgaccgaca cgaaactgag tcgtaacaaa
    19861 atccgcatct ttcaatgctt cttcccgatc cagagttgca tagatcttga cttcatatgg
    19921 agaagcatcc cacatacgct gagacatctg actaacgatc tcaagctttt cttttccttc
    19981 ttctacatct acgagccaga tttctcgtat cggtaattcc tcatatcttt tgataaatcc
    20041 ttccatcaac tcaggggtgt aactgctgcc tccaccaatc gtagcgattt tgattccttt
    20101 tgccatgttc tcactccttt acttttttac actttcatta tcttttttgt aaacaaaaca
    20161 aacaatagaa atccgtttct ttccagtaac atttacaaaa gttactgttt tctagtggat
    20221 ttttgtaaat atcttgtcaa aaaaagccta ttttaagtta tatagtataa atccgt
//
LOCUS       DAXWOO010000040        18887 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.040, whole genome shotgun sequence.
ACCESSION   DAXWOO010000040 DAXWOO010000000
VERSION     DAXWOO010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.040"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(15..725)
                     /locus_tag="ACGE70_001134"
     CDS             complement(15..725)
                     /locus_tag="ACGE70_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFF8692784.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(743..1240)
                     /locus_tag="ACGE70_001135"
     CDS             complement(743..1240)
                     /locus_tag="ACGE70_001135"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8692785.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(1277..1729)
                     /locus_tag="ACGE70_001136"
     CDS             complement(1277..1729)
                     /locus_tag="ACGE70_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFF8692786.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(1864..2469)
                     /locus_tag="ACGE70_001137"
     CDS             complement(1864..2469)
                     /locus_tag="ACGE70_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFF8692787.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(2919..3539)
                     /locus_tag="ACGE70_001138"
     CDS             complement(2919..3539)
                     /locus_tag="ACGE70_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFF8692788.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(3541..4410)
                     /locus_tag="ACGE70_001139"
     CDS             complement(3541..4410)
                     /locus_tag="ACGE70_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFF8692789.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(4433..4840)
                     /locus_tag="ACGE70_001140"
     CDS             complement(4433..4840)
                     /locus_tag="ACGE70_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFF8692790.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(4859..6148)
                     /locus_tag="ACGE70_001141"
     CDS             complement(4859..6148)
                     /locus_tag="ACGE70_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFF8692791.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(6175..6477)
                     /locus_tag="ACGE70_001142"
     CDS             complement(6175..6477)
                     /locus_tag="ACGE70_001142"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFF8692792.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(6502..8595)
                     /locus_tag="ACGE70_001143"
     CDS             complement(6502..8595)
                     /locus_tag="ACGE70_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFF8692793.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(9084..9848)
                     /locus_tag="ACGE70_001144"
     CDS             complement(9084..9848)
                     /locus_tag="ACGE70_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFF8692794.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(9959..10828)
                     /gene="mdcB"
                     /locus_tag="ACGE70_001145"
     CDS             complement(9959..10828)
                     /gene="mdcB"
                     /locus_tag="ACGE70_001145"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFF8692795.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(10853..11929)
                     /locus_tag="ACGE70_001146"
     CDS             complement(10853..11929)
                     /locus_tag="ACGE70_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFF8692796.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(11953..12546)
                     /locus_tag="ACGE70_001147"
     CDS             complement(11953..12546)
                     /locus_tag="ACGE70_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFF8692797.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(12550..14226)
                     /gene="mdcD"
                     /locus_tag="ACGE70_001148"
     CDS             complement(12550..14226)
                     /gene="mdcD"
                     /locus_tag="ACGE70_001148"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFF8692798.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(14216..14515)
                     /locus_tag="ACGE70_001149"
     CDS             complement(14216..14515)
                     /locus_tag="ACGE70_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFF8692799.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(14617..16263)
                     /gene="mdcA"
                     /locus_tag="ACGE70_001150"
     CDS             complement(14617..16263)
                     /gene="mdcA"
                     /locus_tag="ACGE70_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HFF8692800.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(16270..17181)
                     /locus_tag="ACGE70_001151"
     CDS             complement(16270..17181)
                     /locus_tag="ACGE70_001151"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFF8692801.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            17301..18158
                     /locus_tag="ACGE70_001152"
     CDS             17301..18158
                     /locus_tag="ACGE70_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFF8692802.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(18345..18707)
                     /locus_tag="ACGE70_001153"
     CDS             complement(18345..18707)
                     /locus_tag="ACGE70_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFF8692803.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
BASE COUNT         5252 a         4014 c         3272 g         6349 t
ORIGIN      
        1 ttatttcgat tcttttataa ttctgcttct gattcatctt tggttagtgt gtgtctaaca
       61 ctagttacgc caaatgtttg atcgattttt tccttcaacg cttcgattgc ctgtgcgcca
      121 cgtgccgctt gttcagcatt gatggattca atgaccagca ccgctgcttg tgtttcttcc
      181 gtcagcatcg ttctttgggc ttcacgccag ttgagacagc gacagatcgc tccttgttca
      241 tcatagtaga tgatttcttc cggaagtgcc ggagcatcgg aatctgcacc taaaggcagg
      301 aatgattcgc caccttgggc ttttcctaaa tgcagatttc ctgcgatctt ttcgatatct
      361 tctccgccgc aaggtacgcc aaaagacaag gaaatactat tatagatatc tacaagcgga
      421 ttgattggga aaaattcccg ttcttgattt gcacgtttca gtaaagcttc aatagaagaa
      481 cgtgcacctt ttttagtctt gaatgtggta aatgcctgac gccactgagc aatgacttcg
      541 ttttggctga atgtttcttc tgtaaggtaa tttcttgctt cttttgctgc ctcattcaat
      601 aattgatgat acgtttcttc actttctttt gttacgtggt tatcgatacc atttacggat
      661 aagatttcga tggttgcttc aggaaatact tcccagaaat tggtatccac tattactttt
      721 ttcatctgtt cacacctctt gattagaata attgcttgat gaatagatag tctgtttggg
      781 gatcatctcc catataaaaa acatgttcgc tgtatttgat aaatcctttt ttacgataaa
      841 aagcgagcgc aacatcgttg tgttcccaaa caccgagcca aatggctgtt ttttgctttt
      901 cttttgcttt ttcaatagct gtatctagta aaaatgtacc gataccttga tgtttaaatt
      961 tttttcgtac atagattcgc tcgatttcta aaaaatgatc agccatttcc tcagattgac
     1021 tcgttcctgt atttagtttc aggtaacccg caggctcttg atcgatactc actaggaaaa
     1081 aatcggtttc tttatctttt agctcttctg taagtttttc tagtgcataa gcttgtgtca
     1141 agtaatcagc taaatgctcc ggtgaattat cttttccaaa tgtatctgta aatgtttcga
     1201 tactaatttg ttgtagtaag tctacatctg aaggcgtcat ttttcgaatt tcgtatttat
     1261 tcacggcttc ttcctttcaa tagatccttt tattgccttt tttgacaaaa ttccaatctt
     1321 cgctaacatt cttgcatact ttatttaaaa gatattccaa ttgttttgct tcttcgtcag
     1381 aaagaccatt taatgcgact gcattggagt agttgttttc tcgaatgatg aaaggataga
     1441 tttctgctcc ttttttagtc ggatagagtt tcttgatttt tttattttct ttatcttcca
     1501 aacgttcgat catcccattg gattctaatt tcttgattgc tcgggcagtc gttgtacgat
     1561 ctactttgat catttctgct agtttttctt ggatgattcc aggattttcg caaatacgta
     1621 ctaaataaag atactgtcct cttgttaaat cgacttcttt gaattcgata ttgctaatag
     1681 aatctagcgc tcgggcaatc attccgatgt ctcgaaggat ttcagccatt tcatcccacc
     1741 tttattttgt ttgtaagact agttcacctc tttttgttgc aaatgcaact attttcaata
     1801 aaaaaatcaa gttctttttt aataactagt aaagtatatt cttttcttct tttacgaaaa
     1861 agctcaatca caatagatct ggtttttgtt ttctaaaata agtaactgtt tcttcgttgt
     1921 tcgcttttct gatagctttg gcaactcttc tatactaaaa aatcccatgt tggaagtttc
     1981 attattctca ataaacttgg cctcttcacc gtggatcgca caagcaaaaa caagtttata
     2041 atactgaaaa agctgtggaa tatctttttg tttattcgta tcaaaaactg ctcgcaactg
     2101 agtagtttct actgttaatc ctgtttcttc atagacttct ttacgcacat tttcttcagg
     2161 agacaatcct atttcagcaa atccaccagg aagtgaccat tctttcgtcc gtttatcttc
     2221 gattagtagg accttgccat cttttttgat ataggcacga acatctactt ttggtgttgg
     2281 atgtccttct ccaatcgttg tgagcgcttt tatttcttcg aaagtttctt ttcctatttc
     2341 actgatcagt tccaatgaaa tcgtccggat ctcctcaaaa cgctctttat caaatacatc
     2401 ttttccataa tatagaccag cttgagaaag tgcgagtagt cttttgtatt gagccaactt
     2461 cttgttcatt ctcttccctt cttttttagc aaatgtatga ctatgtaatc aagtatagaa
     2521 gactggttta attacggcaa ataaatataa tgaaatctgc aataaataaa agaatgttaa
     2581 aaaactcgag aacaaaagac attcgacttc tctggggctg tgaataaatg gcgttcaaaa
     2641 gcttgcatct gcggtaataa gccccaccaa acgaaaaata tgacgagcta ttcttgtctg
     2701 gctgttctta ttaattgagg caggacagct ttttcacaac ctccccacta aacggtgttc
     2761 tcctaccaac tcctcggtca caagtcacaa tattcatcaa acatgagaag ctgttttttt
     2821 gaaattgctc cttgtgactc ggagctgaac gacaggctca cgacctctcg gtaaacagcg
     2881 ttcaaaagcc aatttttcga tattaagcca tttctttgtt acttattgtc attgatttct
     2941 ttttctaatt tatccaatag tggagtgtaa tccacttgag tatagtctgt tacatcaaat
     3001 tcatatagtg ttcctaactg gaatttgtca tattttcggt cggcgatgat ctttttgaat
     3061 gcttctttcg taatatgatt ggtccctttc gttcgatctt ccaatacgtc accatagtga
     3121 taatgatcca tgatatagct caaatgacga tctgattcac gatcacgttt gtaccgacgt
     3181 tgccataaca cttcaaagtc tgctgttagg cgaatcgtga tcacctgata tccgtatcta
     3241 tctgctaatt cttggagtcg tgctttttgc ttatcactaa aaggatactc ggacacaatc
     3301 actttctttc ctgcttccat atacaaatca agtgctttgt aatagaaagg ataaactttc
     3361 gtcctttctt gttccgcccg ttcttccaaa ttgttaaacc cgatggattc tgcatagtat
     3421 tccttgattt catcgagcgt gatggcatac atatctggaa atcgttcccg tagtttattc
     3481 ataagatatg tttttcctgt accaggacta ccagccagaa gaatgaaata ctttttcatc
     3541 tcatttattt cctaagttaa ttggactttc caagttcata aacagtccaa tcttatgctt
     3601 gacaatttct tttactgctt catttgctgg aatcacatta tggcgaagag acttgttgat
     3661 ttctgtttca ctaaaggttt ctttagctac tttcgtccag ttgactaaaa gctccgttcc
     3721 aacattgaat ttgcggatcc cgttattgat caattcagga taatcttctt ctttcacacc
     3781 tgttcctcca tggataacta gggggacatc aactactgca tcgatttctt tcagcaaggg
     3841 aatgtttact tctgtttttg atttgaattg tccatgattc gtaccgatag caatagctaa
     3901 tgcatccacg cctgtagctt ctacaaattt cactgcgtct tccggtcttg tatatacctt
     3961 gtcattttca gcgacatgga ttccttcttc tgtaccgccg attgttccaa gttctccttc
     4021 tacggaaaca ccgtgcgcat gggcatattc tactactgct tttgtcttca ggatattttc
     4081 tttgaaaggc agatgggacc catcatacat gacagaagta tatccgctgt cgattgcttc
     4141 tttgatatca tcaaagtctc gggcgtggtc taagtgcaag actgcatcaa ccatttcttc
     4201 ttctgcaata gctgtcatta cattgactaa cacatgcatg ccgatatatt tagcggtatc
     4261 gacacttgtt tgaataatga tcggcgaacc catttctttt gctgcgccta gcatagcagg
     4321 aagcatttct aggttatgcg tattaaaagc tccaactgta aaattcaact cttctgctgt
     4381 ttttgttact tctttcaatg ttgtatacat cttttattgc caccttatct ttttatttgt
     4441 ttcctttgat cgttacttgt tttgcaattt gtcgcatctc atccattttg cccatgtagt
     4501 aatcgatccc ttgatcatca ccagcacgag cagcatctgc acgcttctgg ttatagaggc
     4561 tcatcagttt tttataccca tctccgatcg tctgtttatt ttccaaactc ttttctaaat
     4621 attggatggc ttgatcgttc acatacaatt cagaaaattt aagtccaact tgttcataaa
     4681 gtttcgctaa tttatgctta tcttgggtag tggaaatctc ttgtttcaat tgtgtaatct
     4741 ctgattgaat cttttcggtc gcttctggcg atagggcttt ttcttctttt gccagaactt
     4801 ttttcggttc tttttctttt ttcttaaaaa atccaaacat cattttccct actttcaatt
     4861 aaaatgcttt caaaactggg cttaagatcc aggcatataa gagtgctgcg gctactgtat
     4921 ctacatcggt tgctgtagca ttgttgaatc ccatcgaatt aaagatcgtt actagcaaag
     4981 cgggcaataa tgtaataaag aaaccatgag caataccgcc aatgatcgct cctcggcgtc
     5041 cgccaactgc gttgccaaat atccctgctg tccctccggc aaagaaattg gttagcatac
     5101 caggtaaaat catcgctaag ccgaacatgg gcaaagtaaa catggcaata atcgtaccaa
     5161 tcgttgtcgt aatgaaacct aaaatgacag catttgggct ataaggaaag aaaactggac
     5221 aatctaaggc agggatcgca tcaggaacta atttcatagc tatccctcga aatgccggca
     5281 cgatttcacc taacagtaga cgtacaccag ctagcaatac atacacaccc actacgaatt
     5341 ggatggcttg taaaaaggca aacatgatat agttttgatc tcctgataat tcagaagcga
     5401 atggttcgcc tgcaaataaa acagtgatga tatatagcgg gaccatcacg accatgacag
     5461 acaaataagt atcttgcaga aattcgaaat gtgtcggcaa cttgatatct tcgatcgact
     5521 ttttattttc gcctttctca ccaaaaagct tagcaacacc ggcttcaaat aagtacccga
     5581 tcgtacaaaa atgacctaaa gcaatatcat ttgatccagt gattttacga atgatcggtt
     5641 gcgctactgc cggcatagca atggcgaaac aagctccgat aaaaccaccg accaagatca
     5701 atacgatccc tcgcagaccg gcaaaatagc caaagactgt cgtcatcgtt gccatccata
     5761 aaattgcttg accagttaaa aaaatgtact tccattttgt gaaacgagcc aaaataatat
     5821 tgaatacaaa aatggctaag aacgtcagtg caatatctct acctaaaccc aaatcattca
     5881 ttgcttgacc attgatagat tcaatagaag ggatgatccc ttgcatatga aatccttcag
     5941 taaagatttt cccgaaataa gttaaacttc caacgatgat gctcgaacca gcactcaaca
     6001 cttgaaatcc taggagtgtc tttaacgtgc cagagatgac ttgtcctgct gatttcttct
     6061 gcagtagtag acccaacata gcaatcaatg caatggtgat cgatgcctga gtcaaaatat
     6121 tttcgatgat aaaattgata acgcccatgt ttttaccctc ttttcttttt gtatttagat
     6181 aactccctta tcttgcagaa ctggggtgat tttttcttca atttcatttt ttgaaacaat
     6241 atttttcaaa tagatcatcg ccgttttagg atcaactgcg aacttttcga actgcgaacg
     6301 aaaattttct gctgtgatga tcacatctgg tttcatggaa actgcggaag aaatatcaca
     6361 atggtccagt ttggcttcta ctcctaattt gttcaaaaca tcctcagcag acatctgtgc
     6421 tgcaaaacta cttcctaaac ctgcaccgca tacgaataaa atgttcaatt tgttcatctt
     6481 tgttctcccc cgtatcattt attatttctc aactactgaa aaaagcagtt gtttgaaatc
     6541 ttcgatattt gtgcattcaa tcaatcgatc caacttctct tcgttgttga tcaattcaat
     6601 tagttctttc atgatgttca gatgcgagta ggagtctact gctgccaaac aaaaaataat
     6661 ctttactgga tccatttcag gattaccgaa atcgattggt tgctcaatcg tagctacgct
     6721 gactcctaat ttattgaccc catcttctgg tcgggcatgg gctagtgcca tgtgtttgcc
     6781 aatcacgata tacggtccgt aatcattcac tgcacgaatc atcgcttcga catagcgctc
     6841 ttcgacaatg ttttctttaa ccaatggtct agcagctagt tcaatcgatt cctcccaagt
     6901 agtggctttt tctttgatca tgatattgct gtccgtcaaa atatctttta acataggctg
     6961 gatctccctt ttgttgattt ctaaattgtt tgtttcgaat aatttttcta acttgccata
     7021 gatgattcga tctactcggc caccactttc tttcatgaca tctattattt gagtgaataa
     7081 ctccgtcgat tcatgactgt taggctctaa ccgctgtagt tcttcatttc ttttcaaaaa
     7141 gtcttctata accaagatat tcgtctcagt taaaatcgga tcgacaacta aaagcggctt
     7201 atttcgataa ttcaattgaa aggtgctgaa aaccagattg acatccaatt tatctactaa
     7261 ttcgatttct tttgaactta aaactgcagt aatctcgatc tgaggaaact tttctttcag
     7321 attctctgct aacagatttc cagtagccat cccatggtta cagacaacga ctgatttata
     7381 aacataattc aagtctcgct tgattacact ggcgatcgtc gaaaagtgga tgacaagaaa
     7441 agctatctcg tcctccagta tcttgccacc aattacttct tcgatcgtag gaacaaaatg
     7501 agtgactgcg cggtagattg ggaaataatt tgttcggatg ttttctttca atggattcat
     7561 gacttggata tgattcttga ttcttacaat cagtgcagcc atgtgcttat agagattttc
     7621 gaataaagct tcttccttta gatggaaagg tacgtgtgtc tcgttctcta caaattggat
     7681 caattggatc gttagtaatt gagcttgtac ccactcgatt gaattggaga tatctctagc
     7741 attgaacgtc tcaatagtga aacgaagata atttctttct acttgcggga cattgtccaa
     7801 ttgaaattcc tcgatcatgt ggtcaataaa tgcagaaaag ctactgtctt ttatttctgt
     7861 atttttccaa ttaatggcag agataaattc atgtttcctg attcgctgga tccagatcaa
     7921 tgtaaaaagc aggatttgtt ttttataaat atcatcttct tgtttcaaca acttattgga
     7981 atagatctcc tgtatttttt tgatttccac caaaggaaaa taatggagaa agattttttg
     8041 agccggtgtt aattgtgcat cttgattcat aaagaagtcg gtcattccca gattctgatt
     8101 gataaaatca tagatcatcg tacggataga tcgttctgaa cctttataaa ccagtccttg
     8161 ctttccataa ctgacgatct ctatcccata actgaccagt gttgctcgta accgtctcat
     8221 atcttcatct aatgtacttt tagagatttg aaattcttct tcttttttgt ttagatggat
     8281 cgggattcgg ctgtaagcaa cggctaaaac tagatcaaaa atacgttcgc gccgattcaa
     8341 aaagccgtct ttcacatctt ttatttcagt ttctaatcga gcttgctttt ccttttccag
     8401 cttcaacaaa aaacctttgt tgcgaattgt acggatctcc ggaagctttt tactgctcaa
     8461 ccagatattc gcttcaagaa cttcctgacg gatcgtccgc tgactgacat tgaactcact
     8521 tgctagatgt tttgctttta ctggatattg ggctgtcatg attctgtgaa gtatttcaat
     8581 tgttcttgtt ttcatagaaa tgatctcctt ctgatttcaa gtatatggtt ccgcttacat
     8641 aataaaaagt agatatttct gccacttgaa gtggaaagaa agtaagcaaa gcaaacaaaa
     8701 aataagccat atgctttttc atctcctcaa cgaaaaagac atacgcctat tattacaaaa
     8761 attttgccca tctatacaaa aaagaggttg tgaaaaagct gtcctgcatc aagtaataag
     8821 aacaactaaa ctaaaatagt gcttcctatt tttcgtttgg ttgttcttat tatcgcagat
     8881 gcaagctttt aaacaccatt tagtggagag gttgtgacag agatgttcag ccttgagcga
     8941 taagaagcga ttttgaaaaa tcgcttccca tattttacaa aatcgtgcct tattgccgat
     9001 aggctatctc tggcacaccg tttattcaga aaaaggagct gcgacaaggc ttttgtcaca
     9061 actccgttca taaatatttc ctgtcaatcc agcataatcg gctgaataaa atcggcttgc
     9121 catctttttt ctttctcaga aaaatcaaaa taccatatct gagaaaaata ctgtcgttgt
     9181 gtcttacttc ctgagatcaa tcgcccatct tttcgatcga ctgaataatc acagcatctg
     9241 aaatcaattc ggatggcaaa actattttcg tggatctgtc gataatttcc tacttcctga
     9301 atagttattt ttttagtatg atttcttaca ccgtctgctt tgtttttttg cagaactttc
     9361 tgatgttcgg caaacagctt gtctgtataa cagcttctgg ctttttcagg agtttcttct
     9421 tcccaagcgg attggatctt catgaatatc ttttggattt ccagaaactg tgctttcttt
     9481 tctttagccg ttcctgacat gcgggacaat atctccgctt tcagcctttt cttttgtctt
     9541 ttattcttga cgatagaaaa agcagaaacc cctcctactg acaaaacagc taatgcaaga
     9601 aatcccgttt gctcataact agaaggacct gtacctggac cataataccc ataagaatct
     9661 ctgccgtaag aacgactgcc aaaataactg cccccatatg aacgaccacc aaatgaacct
     9721 ctgcccgaac ttcgaccgct agatgtacct ccaccatgac cacccgcgac agcgaatcct
     9781 tgggtcggca gagcaaacaa acataggccc actaacaaaa tgattgctaa ataattgatt
     9841 tttttcattg tcttcctcgt tatcttttat tacaaaaaac tatttttatg atagccttgc
     9901 taaaaggaag agtcaataac cagatgcata aactgttttt ttagcaattt tgtcttattt
     9961 agtcattgag taacatcgga caaatgtatg tgtcggcagt ccataattcc tctatcttca
    10021 gcatgaacaa gcttgcagac aagagatctg cgcttcctcc aggagagatt tggttttcct
    10081 gacaaaagtg caacagtgct gtaaatggac gatttggtaa agcactttga ctagcagcat
    10141 gagcaagttt ttgcatttcc actaacgttt tctgatcact gcgatgcaat acgcatgtgt
    10201 catctagcga actcatcaaa actaacaaca tatgcatttg tttctgtaca tatgaatcat
    10261 tatctagctc cttagctgct tgaattgcaa gcctgatatg cggataccct aatatcgctt
    10321 cttcatatgc accatttacg ccatattttt gcttcaccct atgaccatga gtggtttttt
    10381 tatgacgata ttttgtatca ggaaaagcag caagcttgct gacatcatca agtattttcg
    10441 ataaagaaaa aggctgttcc atactgattt tttgtgcaat gacactgatc aatagcccca
    10501 ttgcccaaat cgctccttta tgggtattga tgccatttgt agcaatcagc attgcctttt
    10561 ccgcttttcg cccaatctca gcaattcttt ctcttaactc ctgatcaata gcatgatgcc
    10621 atgctatttg agccatttct tcaaaaaaag gagttagcgt cttagcagac ttaagaaata
    10681 aagcatagtc catatcggaa tgtgcacccg catccagtgc atccactaat ccaggttttg
    10741 gacttaatgt tacttctgcg atcaatgctt cctctaccaa cgcagcgagt tgctgagtca
    10801 ctgctttttt tcgttcgtca tttatcatga cactcactcc ttcttcctac tattacttgc
    10861 tgaaaccttt tttgatagct acccaaatga tatgaagtaa ccagcttaaa gcaaatgcac
    10921 aacttgccgt cattagataa gctacaagaa tcatgactcc tgaaggtaac tgcagccaac
    10981 tatctttaaa caataataac agtacttgga aaacaaatac attggctaaa tacgtacgat
    11041 aagcataaac agacaaccat ttgaacaatg gcaattttgg cgaatcgttt acaatcattt
    11101 tggaagcgcc tatgaagaca gcaaagatga tgactaaact gtataaactt tggatcgtat
    11161 tcaagtaagg tgcattcgca aagctcaagt ctcctggatg tgcgagcaat gaacggacag
    11221 aaaagaacgc gataactaag gcaacaggta agaaaagata acgtactctg tccaaatatt
    11281 gatagacttt ttcgtgataa acaaatgcgg ctaccccata aatcatgaaa ctagcaacaa
    11341 accgatctaa taagtaccag ctgtcatgat aaggcccagt caatacttgt gtttgataga
    11401 acagatacca tgcgacatat aaaatcgttg aaccaatcaa tactggccat acatattttt
    11461 tattagcaaa tactttgtaa ccgagccaaa caaaaaacgg catgaacaat tggatctgca
    11521 gcatcataac ggtataccat aaatgaggcg ctccatcacc tgtgacatat ttcagtaaaa
    11581 agcttgcagt atccgtatac ggagtttgtt gttggactgc tggaaatagc caaagataag
    11641 ccgtcgtcca tagaagatac ggaacgacta gttcaaaaaa cttttctttc aaaaattccc
    11701 aataagacaa atgctgactt gtttttacca tattataaac aattgcaaaa ataaacattg
    11761 gtgctgtgta tttaacgatg acgtaaaagg taccaagaaa agctgtattt gttgcgctgt
    11821 ggctgtttgc caaagcaaag ctcaatatcg tttgcatcat aacagccgtt acagctgata
    11881 ctttctggaa gtcacttacg ttgattttga tttttttgtt ttcaaacatc tctttctctc
    11941 tctttttctt tattaccaaa gttctttggt taatttaggt ccttcgactg ttttcaatag
    12001 aatttttttc tctggctctc tcaaatactc ttttagtgaa aaaccgcctt gccgagtcac
    12061 tacttgggta tcgaattgct caaaaaaagc aggatgcgat tgaattacct gaattggcaa
    12121 ttcaatcggt gaatttgcgt acagcagcaa atcaaaatca ctggtttctt ttactgccgg
    12181 aatcccagtc gataattcaa atcctaagct gccgccgatt ccccatttac atcctgataa
    12241 tatgtttcta gcagcatgaa attgctgata aacaggataa tccatgattt tctcttgtct
    12301 atacgattct tgttcgatga tgtcccacgg atgaatgact tgtttgacgg catgttttga
    12361 gatttctgtt gaccatctct gattgcgggc aggtccacgt aaaccaatcg gtagtatttc
    12421 tgcgtgatcc gtcgacgcac gacgaactgt tccgaacatc ttttcagtta gaacgaccca
    12481 ttcaggcaag gtgaaccctt ctatttcatg tttatcaaaa aggacaatat catgtgcttg
    12541 aatcattttt tatgcatccc attcttcgtc cattttggca cggactttat ttgtttcgac
    12601 acgtccgaat gttttggctg tttcgtttgt gtatcgattt gtcagcatcg tgtcatttgt
    12661 attgcggaca tcttgaatgg cttcattgat tgcttcgatg actttgctga cattttgttc
    12721 tgaagcagct tggtcggtga tatcttctag gaaatgatag atcgctccta gttttgtata
    12781 attattgata tcgtaagcga tagctggaac tttttcagca gctgcttcga tttcagcaac
    12841 cgaacgtttt gtcacacgtg cagcactttc tttagacatc gcttggacgg taatttcttt
    12901 attgtttaaa gcaatcaagc ggcttgattg taatccatgt gctaaaaatg cacctgaaat
    12961 agcatttcct gcaatcaatc caataactgg atgtccagct tgacggagtt ttgcataagc
    13021 agcagcacta ttagccaaag ccacatggat accgatcaat tcttcgttat agccgtatgc
    13081 ttggcttggt acatcgatca caacgatgat tggccgtttc acttttttgt ctttatcttc
    13141 ttcgtaaacg tgatccaaaa tttgtcccat gcggaaacct tcaactaaac caacttcacc
    13201 gtttcgtaca cggtacatcg ggtttttagg atcaggtacg attgatgtca cgatggcttc
    13261 ttctatgaat ttacttgatt taccataata cacagtacta atagaagaag ttggattttt
    13321 catgccagtt agtgccgcaa accaatcgta accaacactt gctgcagctg gttcatttcc
    13381 ttctgctact tctggtaagt caattgattt tgcttgatgg ttagcaattg cttcattgta
    13441 atcgtcaatg gccattggtt ttgaaagatc taaagaatca agtaatgata aataaaatgc
    13501 tgagctttca ctgcgatgcg tgtcttttcg ttcgtccatt gctgcgatga cagcagcttt
    13561 gattgcttct acactatcag tcactaattc atcaatgatt tttgattcta cccgttgacg
    13621 agatcctaat gtattccaaa tcaagtcttt gtcactagaa tcaaattctc gaacgcctgc
    13681 ttcttgttca ataacttctg gtccattcaa accgactctc gctttttttg ttgcgattaa
    13741 ataactcatt aatgctgaag taatcgacat accgccaaat gatcctacac gtcctggaat
    13801 caaaccgatg actggtacat attgttttag agcaatgatt acatttccga tttctgaaat
    13861 agataacagt ccgtagtttg cttcttgcaa acgaacacca cctgtatcta gtacaatgac
    13921 tggaaagatc tcatttcctt ttttattttc ttctaacaca tgttcaagtg cagcaacgat
    13981 ttttgcaccg gaaacttcac cgataccgcc accttggaat tttccttcca tagcgattac
    14041 tactgcttgt ttgccattga tcgtgccacg gctgacaacg atcccgtcat cactttcagg
    14101 gacgattcct tgtgctggta aacttggcgc gatgatttga tcgaatggat caatcaattc
    14161 acgtccattg tcaagtagag cgttcactcg ttctctggcg tttaattcta caaaactatt
    14221 tttcatctga aagttcctcc attgcttgcg tcaatcgtag gtacacaact cctggtgtag
    14281 caccgaagtc attgatcacg atatctgctg aaattggata acgatcaaaa aatctcgtta
    14341 atacattgtt ccatacttcg ccgaagccat cgcttcctgt acaaattttt acagtagatt
    14401 ctgttttttc tgttggaaac atcaagattt ccaaatctcc tgaaccgacc actcctacat
    14461 ggattttctt attgatcggc tgtttcgttg gatatttata agctaattgt tccatggatc
    14521 tctccccttt ttgtaaaaca gagcatacac gttctgctta ttcgtctttc ttcttccatc
    14581 tgggtaaatg gcgacaaaag gtttttctcg ccaattttat gaccaagttc tgaatttcat
    14641 tggtggttca tacaggccat ctgaccattc gatcaaatca tcgatggttt gtgcagctaa
    14701 taatgaacgt ttcgcttcac tacgtttgat gcctagatct tctggtaatt tcacgatgcc
    14761 ggctgcacgc aagctatcta gtgattcttt cgtgctagtc attccaagag gtgtaacacc
    14821 gccaattgct gcaattgctg cttgtcgttc ttcttttgtt tttgctttgt acaaataagc
    14881 aattccttct tctgtcacga tatgtgtagt atcttcgcta taaatcatca ctggtgcatt
    14941 ggctaagcct gcttgttttt gaacagctag tgcatctaat tcttcgacaa agactggttt
    15001 tttatttgaa ccatatgttt caaccatttg aacaactaat ttttggcctt tccctaatgg
    15061 atcatcattg tcgcgaagtg attgccatgc tggtgtagaa tgacgacgac cgccaggatt
    15121 atgtcccatg ttaggcgcac cgccaaaacc agataaacgt ccttttgtta cggtagaaga
    15181 gtttccttcg taatctaatt gcaaggttga accgataaac atatcgatgg catattgtcc
    15241 tgcagcttgt gataatgttc ggttactgcg catcgtgccg tctttaccga caaaaaagac
    15301 atctgggcga gcagcaatat atttttccat accaacttcg ccaccaaaac tatggatgct
    15361 ttcaacccaa cctgattcga tggctggaat catcgttgga tgtgggttca atacccagtt
    15421 cttcgcgatt ttccctttta accctagtga ttcaccataa gttggcagta acaattcgat
    15481 agctgctgta ttgaatccaa taccgtgatt caaagattgt acgccgtgtt ctgcgtagat
    15541 tcctttgatt gccatcattg ccattagaat ttgcaattct gtgatgtttt gaggatctcg
    15601 tgtgaataaa gcttctaatt gataaggttt atctgctttc acgacaacgt ctacccagtc
    15661 accagggata tctacacgtg gtaaagaatc gacaacttcg ttaacttgaa cgatcacgat
    15721 accatctttg aaagctgcag cttcgatgat tgttggtgtt tcttccgtat tgaaacctgt
    15781 gtacaagttg ccgtctttat ctgctttatc tgcagctact aaaacaacat ttggaatcaa
    15841 atcgatgaat aagcgtccat aaagttccaa ataagtatgg atatcaccta atttcattgt
    15901 gccgtcttca atcatttggg cgacacgcaa gctttgtgca ccagcataag agaaatcgat
    15961 cttgcttgcg attccttttt caaaaatatt caaatgttct ggtcgtgaga tactagacat
    16021 gatcaaatgc agatcattca ctttttctgc atctactttt tcaagacttt ctgaaagaaa
    16081 acttgcttgt ttttgattgt cgccttctaa tacaactttg tcgccgggag caatcagttt
    16141 ttccattact tctacgattt tttcagtttc tacatacttg ccattgagca agttgctgat
    16201 tttttccaat cgctgcgctt ttttttcgcg tttagaagcc cagttgcggt ttggtttttc
    16261 cataattagt catctccatt tttgatatag aaacgtggcg tcatcaatac catgttggtc
    16321 tagattgatc gtacggatcc ctttatctac accaaatttt gtatgaatca gctttgtcaa
    16381 agtattgccg gggggaaatt caatcgtcag atccattcct aattcttttg ccaagtccat
    16441 catttggttc cactgcacag ggaaagctag gttttccaac aaatctttac gaatctcttc
    16501 tgcgtcctga gtggctcttc catccgtatt ttttatatac aaacatctag catcagatag
    16561 atggacagac tgcatatacg gcttgaattt cttgaccgta tcattcatca agagacagtg
    16621 agatggcaca gggactttca gcagcttcgc tgtttgcgct tggtgccgtt tggcttctgc
    16681 caacgtaacg gataaacctt cgatactccc agataacgta tgttgcatcg gacaattttc
    16741 atttgacaga taaactggat ggtcaggatc aaatgttttt gctacaattt cctcagcttc
    16801 gcttctcgtc acacctacta ctacgcccat tgcataaccg cttggataag cttctttcat
    16861 taagactgcg cggtgataga ctagttccgc tgcattctca aatctcaaag atcgacaagc
    16921 aacagctgca caaaaagcac caatcgaatg tcctgctgca atatcaggct gaataccgtt
    16981 tttttccatt tcatctaaat aaaatactgc ttttgtcagt aatgctagct ggataaaaac
    17041 cgtatcttga tagttttctt ctttatcaac taaccgatac cccgtcacat tttctaaacg
    17101 ttccatgtac atttcaggga tatccgctaa catccctaac ttctgactgc cttgtccagg
    17161 aaataagaaa cctgtactca tgagcctcgc ctcctcagca cttacttttt ccactgctat
    17221 cttagtcaat ttctgctata ataaaaaatg ccaaaaaaag aaaaaaccat aacaaaaagg
    17281 tatggatggg atggataatt atgaaattgc ttcatctgca gtattttaaa aaagtattcg
    17341 aaacaaaaaa tgttacccaa gcagccaaag aattatttat ttctcaacct gccttgagcc
    17401 gtgcgatcaa acatttggaa aacgaattag gtgtcccttt gttttaccat agcggcagaa
    17461 acattgagac gaccgtttat gcagaagaat tttatccata tgcagtcaag acattagaca
    17521 cattagaaga aggaatcaaa ttattgtctt cggtcaatga gaagatcgtt acttccgtca
    17581 tattgtattt agaggtagcc tctgtttcta ttccgaactt ggtccgaatt tttatggaaa
    17641 agcatcctga tatccaatta acgatcatgc aacatcattt accagatgat attaccgatc
    17701 ctgtactgta catcacttcg gagatgaagc ctggcatgac caatattcct atcatgaaag
    17761 aacctgttta tgtcgccatt cctaagaaac atcctcttgc taaaaaagaa catctgcaat
    17821 tagaagatat ccaacgcacc cccatcttga tgctgtcaaa gagcaacgcc ttccgtcata
    17881 cactggatgc agcacttgaa gcgaaaagta tcgaattgac gatcagttcg acgacagatg
    17941 atcctgcgac attacgttct attgtcaagc aaggattagc tgttagcttt ttcccaaaag
    18001 tttcttggtc ttacgataaa aatgatcctt tcgtcttgcg ggaaatcaaa gatttgccct
    18061 tgacacgaac aatttattta tcttctagct tcacagaaga tcatcctttg atcaaaatga
    18121 ttgcaaaaac gatgcgggaa ttttatttag gaaaataaaa aaaaaagagc tgggacaaaa
    18181 ttcttgtcgc agctcttttt tcgaacaaac ggtgttccag aagtaatttc ttagaaaata
    18241 agccgaaatt tcccaaaaat ttgagaagca attttcgtaa attccttctt atttcttgaa
    18301 gctaaacatt tctgtcacaa cctcttctct attgtcctag acattcattg cgttgattta
    18361 aaacttccat caatcaaatc tgctagttca tctagctttt ctattttacg tagatcaata
    18421 gagatccaat gttctttatt catgtgatag gctggaaaat acccctcttt tcctcgcaaa
    18481 attccgccaa gttcaggatc gaccttcaaa tcaagaatat tgatgatcct atggtcactt
    18541 gcatcatcat agattttttc ttctggaaca gacatcagga ccccaaacca ttttttgctt
    18601 ttttgatgtc tgaatacgca ataatcagga aattttttaa aaatatattc tggagacaaa
    18661 ttgtatgttt cattgatgtg atcgattagt tcttttttcg ttagcatctg cttccatctc
    18721 tttcttattg attcgcttta ttcaaagata ccacgtaaga atagaaaaat gaatacaagt
    18781 agttgctaat caaatacttc gatatttttc ttttttatca actcttaaat gttcaatccg
    18841 cctgtgttgg tcttgctacc atctactatt aaaatgttta ttattgt
//
LOCUS       DAXWOO010000041        17989 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.041, whole genome shotgun sequence.
ACCESSION   DAXWOO010000041 DAXWOO010000000
VERSION     DAXWOO010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17989
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.041"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            214..498
                     /locus_tag="ACGE70_001154"
     CDS             214..498
                     /locus_tag="ACGE70_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFF8692804.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(779..1198)
                     /locus_tag="ACGE70_001155"
     CDS             complement(779..1198)
                     /locus_tag="ACGE70_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFF8692805.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(1200..1784)
                     /locus_tag="ACGE70_001156"
     CDS             complement(1200..1784)
                     /locus_tag="ACGE70_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFF8692806.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(1831..3306)
                     /locus_tag="ACGE70_001157"
     CDS             complement(1831..3306)
                     /locus_tag="ACGE70_001157"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFF8692807.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            3487..3798
                     /locus_tag="ACGE70_001158"
     CDS             3487..3798
                     /locus_tag="ACGE70_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692808.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3914..6250)
                     /locus_tag="ACGE70_001159"
     CDS             complement(3914..6250)
                     /locus_tag="ACGE70_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFF8692809.1"
                     /translation="MLQLKDIKKYYKVGEPTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            6392..7060
                     /locus_tag="ACGE70_001160"
     CDS             6392..7060
                     /locus_tag="ACGE70_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFF8692810.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            7063..8106
                     /locus_tag="ACGE70_001161"
     CDS             7063..8106
                     /locus_tag="ACGE70_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFF8692811.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(8343..9224)
                     /locus_tag="ACGE70_001162"
     CDS             complement(8343..9224)
                     /locus_tag="ACGE70_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFF8692812.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            9383..10111
                     /locus_tag="ACGE70_001163"
     CDS             9383..10111
                     /locus_tag="ACGE70_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFF8692813.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            10267..10584
                     /locus_tag="ACGE70_001164"
     CDS             10267..10584
                     /locus_tag="ACGE70_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFF8692814.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            10577..11185
                     /locus_tag="ACGE70_001165"
     CDS             10577..11185
                     /locus_tag="ACGE70_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFF8692815.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            11240..12031
                     /locus_tag="ACGE70_001166"
     CDS             11240..12031
                     /locus_tag="ACGE70_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFF8692816.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            12169..12657
                     /locus_tag="ACGE70_001167"
     CDS             12169..12657
                     /locus_tag="ACGE70_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFF8692817.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            12708..13580
                     /locus_tag="ACGE70_001168"
     CDS             12708..13580
                     /locus_tag="ACGE70_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFF8692818.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13756..14118
                     /locus_tag="ACGE70_001169"
     CDS             13756..14118
                     /locus_tag="ACGE70_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFF8692819.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            14127..14879
                     /locus_tag="ACGE70_001170"
     CDS             14127..14879
                     /locus_tag="ACGE70_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8692820.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14893..16695
                     /locus_tag="ACGE70_001171"
     CDS             14893..16695
                     /locus_tag="ACGE70_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8692821.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            16972..>17989
                     /locus_tag="ACGE70_001172"
     CDS             16972..>17989
                     /locus_tag="ACGE70_001172"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFF8692822.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTE"
BASE COUNT         5633 a         3126 c         3722 g         5508 t
ORIGIN      
        1 aggaagaggt tgtgagcctg ccgtttaact ctgagtcaca aggagcaatt tcaaaaaaca
       61 gcttctcatg tttgttgact attgtgactt gtgaccgagg agttggcagg agaacaccgt
      121 ttattcgaaa aaaggaactg cgacaagact tttgtcacag ttccttcttt tttttgctat
      181 gctatactag atgtaaaaaa taggagaaat aaaatgaggg aaatttattc ttttgaatca
      241 aaaatctatg cttccgaagt cggcaagggc ggggcatata ttatttttcc gtatgatata
      301 agaaaagaat tcggtgctgg aagagttaaa gtccaagcaa cttttgacaa tgtgccgtat
      361 gaaggcagta tcgtgaatat gggcgtgaaa aatccagatg gttcagtctg ctacatcctt
      421 ggactacgaa aagatattcg taaaaacatc ggaaaagaaa tcggtgatat tgtagcagtt
      481 acagtgaagc aaaaataaaa gaggttgtga caaaagacgt gtcacaacct cttgataaac
      541 ggtgttcaaa agccagtttc tcggtagtaa gtcaaattta tgcaaaaatg tggaaaccca
      601 tgtttacaaa atttttctta ctactcgaaa cgaagcggct ttatcacaac ctcttgataa
      661 acggcgttca atcagctgtc cctcggtatt agctcaaaaa cggcaaaata tgagaagcta
      721 tttttccatt tttttctctc atactcaggt cataacgctg atttcacaac ctcttctttc
      781 ataatggttt ttcaccatct ttataataaa ctggttcttt gatatggaaa tctatttttt
      841 ccaaccaatc taaaatcttg taacctaact cttctgacat cggaaaagag gcgttgattt
      901 gtgcattgga gatatggtca acatggatac cggaagtgat cgtacaactt cccggaacgg
      961 cagttttgat gatcacagct attttttcgg ctaagacatc gtctttatgg aaccgaccat
     1021 cgtggctagg gaaacgtaca gtttgcatct ctgtatcacg agaaaatgta gtgactgttc
     1081 cgatatgtgg tgtatctccg cctgttaaag tgatcaataa gtctttaccg attactttaa
     1141 cagcagcttg gatcgtgaaa cctagtttgg ttacttcaac tatcgtttct ttttccatat
     1201 taatcaccct gccaacgttt tgaatgctca tttacaattt caaattgatc caaaagtttc
     1261 attgtatggt gattaacaag atcttggatc gtttgaggct gattataaaa agcaggaatt
     1321 ggtggtatga tttgcacacc tagtcgtgct aatttagtca agttttccaa gtgaatggca
     1381 tttaatggcg tttcacgagg aacaatgact aactttcgtt gctccttcat tgttacatca
     1441 gcagcacgtg caatcaggtt gtcgcctaaa ccaacagaga tacctgcgac tgttttcata
     1501 cttgccggta cgatgatcat tccatcggtc aaaaatgatc cgctggcgac agcagcacct
     1561 aaatctttgt ttgaataatg gtaatcaaac aggctttcga attcagttag tgataagtcg
     1621 gattccagct ttaagttctg ttttgcccat ggactgatga ttccgtgtgt ttctatcatc
     1681 ggatattctt ttagtttctt cactaggttg atggcataaa tggtgcctga agcgccactt
     1741 acaccaatca cgattttttt cttccgttgt gccaattgtt ccattttccg cactcctttt
     1801 tatgatgtca aaacagttgc agatcattca ttatttcaaa tatttttccc aatcttctac
     1861 ttcttggaat tttgcacgta caaaatgttc tttcatatcg aaaggaactg taccgtcaaa
     1921 gatagttttt gcacttatcc cacggaaacg aatagagcca ggattatatt ctggtaattc
     1981 tgatgggtcc aatggatggt tcctcattcc tgggatcacg atgatatctt tatctccttg
     2041 gaaacgagtg ttgaaagtcc aaactacatc gttcatgtcg aaaatatcaa catcttcatc
     2101 gactaaaatg actgtcttca attctttgaa ggctgataaa gcaagaatag ctgcttgacg
     2161 ctgaatacct tcatccgctt cgttttcttt acggatttgc atgatggtca tcaatttacc
     2221 accacctgca ggaggattgt agacatttac tacttttcct ggaatagctt tatcgaccaa
     2281 ggcaagaata cttgcttctg taggaatacc ggccatagat acgtgttctt cactgggacc
     2341 aatcgttgtt tgcatgattg gattgtcttt tcggtgagta actgctttga ctttcactac
     2401 ttgtacagct ggattagcat ccccagtata gccaggaaat tctggcattg cttttcctga
     2461 gttcgtgttg atatcttctt gcattgtttc atttggcatg atttctgctt caatgatgaa
     2521 ttcagcacga gcaataccca cttcatcgac tgctatacca tcaacgattt gaataggttc
     2581 gttacgcaaa gctcctgcac accatagttc attgtagcca agtggagtgg taggtggttc
     2641 gaaagtcgtg ccaatggcaa ttgctggatc taaaccaata ttgatagtga taggcattgg
     2701 tttattcaag gcttcgaatt gtttttggaa gtgaccaatg tggcgtccgc caggcatgat
     2761 gtacataccg atcgtatctt tatcttctag aaccatacga tggatcgtta catcgctcat
     2821 tgttttttct ggatctgatc ctaagactac acccattgtg atgtatggac cagcatcttc
     2881 aggagtattt gtaggtgcag gcaaaatgtt gcgaatatcg aagtctgcat cggtagctag
     2941 atgaacgact tcttgtgctg gagcgtctgc tttgtctact ttcacaggtt tgattggatt
     3001 ttctacagcg tctttcaaga aacgaccaag tgttcgataa tcatggtgca aaatttcacc
     3061 tacacgttta cgactagcca ttgttccgat gttcacacgt gtgccgggga aacctttgat
     3121 attgttgaaa gtcatagctg gtccttcttg agtcggacgt tttactgtac cgccagcacc
     3181 gatataacgg taaacaccag ataattctgc ttcaggatcg atttcaatat ctgtttcgtg
     3241 atattgacct ggaagtgttt ttagttcttc gatgactttt cttaaatcat acggttgatc
     3301 tgtcatgaga gattcctcct tagttgtctt tacctatcta tagtaaaagg tgaaaaagag
     3361 gaaaacaatt caacttgttc ggtaaagtat tctacaatgg aatggatgcg tttttttgat
     3421 aatagaataa actatgatat acttatcact aaaagaaagc gcatacttag tgttggagga
     3481 aggaaagtga aaaaaatgga ggagtcattt cctaaagcag taaaagtgga aaatatagcg
     3541 aatattttga aagttacatt tgaaaacgga gaagtaaaat acgtgaaatc tcattggaca
     3601 gaagagataa ccgatgcctt gcaatttgga aaaaaaggaa gaggaaaaag aaaaaatctt
     3661 ctcgcacttt ctcgtaatat gtggattggg actgaagtaa cgattgaagc agatggaaca
     3721 gtattcataa atggaaaaga caggtatacg ccagaagagt tgtggtacaa aggaaagaaa
     3781 agtataccag aattataaaa aataattaaa aaaaaacagc gcgaaacaag gaacatatta
     3841 cttcctcgtt tcttgctgtt ttctactatt tcagcgctct tatgactcaa atctaaatgt
     3901 ggttgtcaca accttattct gcacgcaaag cgatagcggc gtcttttttc gctgccattc
     3961 gtgcaggaag atgaccgcca agcatcgtca atatagtaga gacgatcact agaataatcg
     4021 catggacagg atttagttga gcaacatttt ttagatccgt catgttgtat aagatgctat
     4081 tgatagggaa cgtagctagc catgcaataa acacacctaa tatcccagaa gaaataccta
     4141 aaatacacgt ttctgcgtca aatacgcgag tgatgtcttt tttacgtgca cctaaagctt
     4201 ttagtacacc gatttctttt gtccgttcaa tcacagatgt ataagtaatg atactgatca
     4261 tgatcatact tgttactaaa gagatccctg caaaagcaat aaggacataa gtgatagcat
     4321 ccatcaatcc gccagtcagt tcagtcatcg ttccagctaa atcagtataa atgattttat
     4381 cttcatctga tttgccttta ttgtaatcat ctagataatc caagatcttt tctttgtctt
     4441 caaaattgtt cggataaatc atgatacttg aaggcaagct gtctccacct aaataactaa
     4501 gcaatgtttg tttggcattt tcgtcaactt tttcagtagt taagacattt acaccactgt
     4561 ctctttgagc tttgacgatt tctgattctt tgttttcgtt gacgatttga gtcgttaatt
     4621 gatcgctata agcgattcct ggagataaga gattcattgt agaagaagat ttcacgcgta
     4681 ggatccccga gatcttcaat tcatcagaag cattctgata catcgcatca taatctgtat
     4741 tagggataaa attcccagtt ggaagttttg tatagtaagt attgttgttt actaatttga
     4801 atgttgtacc aacgatatca tcaaaatcaa gtttttcgtc ttctttcaca tcaaatccaa
     4861 gattcttcaa tgcgttgata ttagtattat tattgccgtc tacgatcagc acgacatctg
     4921 ttgctgaggc tggataactt ccggcaagta aagaataatt gtcttttaaa aagttttctt
     4981 tgctagtgtc aagctgtgta gggaaacttg acactcccac acctgtcatt gctgacatcg
     5041 tactagataa agaaagagat tccgtatcag gattttgatt cgagaagctg acaggctgta
     5101 ctttgccatt tacatcacgt aaaagattga ttcctgtagt acgggtaaat ccgatattat
     5161 tacttaagtt aggatcgatg tctgttacgt aatcgacgta tgtttgatca atcttattcg
     5221 tgtgctgtgc tttatcttct tcgctgactt ttgcagtaat cgttttgctg tctggataat
     5281 ccgctttgct tgcacccaat ccggcatcgt cacgagtctg gttagtcgtt accttagaaa
     5341 tagtaatcgg aaatttcgcc atcgtttcag cttgcgtgtt atcgatttgt ttttggaatc
     5401 cagaagaaag ggacagaacg atcgcgattc cgatgatccc aatactggaa gcaaaggagg
     5461 tcaagaaagt acgtcctttt tttgttcgga tattgttgaa ggaaagcttt aacgctgtcc
     5521 aaaaactcat ctttgttctg cgtaagttaa attgatcatc ttttggtcgt tcgatgtgag
     5581 gatgggaatc tgttaagatc ttaccatcag aaaattcaat gattcgatct gcgtattgat
     5641 gggccagttc aggattatgt gtgaccatga tcactaattt ttctttcgat aactcttgga
     5701 tcaattccat gatctggata cttgtttctg tatctaacgc tccggttggt tcatcgcaca
     5761 aaaggatatc tgggtcattg gctagtgctc gagcgatggc cactcgctgc atttgtccac
     5821 cagataactg atttggcttt ttatgcatat gatttgtcaa gccgactcgg tgcaatgcat
     5881 cttctgcccg ttttcgtttt tcgtctttag aaacaccact taatgtcatc ccaagttcaa
     5941 cgttttcaat aatgcctaga tgaccaatca agttgtaact ttgaaaaatg aaaccgatag
     6001 agttgtttcg gtaggcatcc cagtcactgt ccttaaagtc tttagtagac tttccgttga
     6061 ttaccatatc accggaatca taattatcta gtccaccaat cacattcagc atagtcgttt
     6121 taccagaacc actaggacct aaaatcgcta cgaattcttt ttgtctgaaa gcgacagaca
     6181 caccatccaa tgctttggtg gtgggttcgc cgactttata atattttttt atatctttta
     6241 attgaagcat aatcaccctt ctcccgtatt gaaatattcg gtatactaat aagtatatca
     6301 gaacaatgat cgtagtatac gaaataaatg ttaactgaag atgaactaca agaaaaaatc
     6361 tagaaatttt tcaaaaaaca gagggagatc aatgaatcgt attttagtag tagaagatga
     6421 tccgcaatta cagttgcttt accgttctgt gttggaacga gcgggctttt cagtcatcac
     6481 cgcattgaat ggagaggatg cgctgaaaaa actagaagcg acacgtatag aattaatcat
     6541 tacagatatc atgatgccga aaatggatgg ctatgacttg ctagactcat tacgtagcac
     6601 cagatttgac acacctgttc taatgattac ggcaaaggcc gattttgagg ataagaaacg
     6661 cgggttcaaa ttaggagcag atgattatat gacaaaaccc atcgatgtca atgagatgat
     6721 cctacgtgtg gaagcattgc ttcgacgttc aaagatcaat tacagcagta gcttgataat
     6781 cggacaaaca aaattagagc tagatgctta tacagtgtta caagatcagc attcgttgat
     6841 tctgcctcaa aaagaatttc tgcttctgta taaattactg tcttatccca acaagatctt
     6901 cactcgccaa cagttgatgg atgatatttg gggattagat acgaatacag aagaacgaac
     6961 gatcgatgta catatcaaac gtttaaggac ccgttttgaa aagacggaag attttcaaat
     7021 catcacagta cggggactag gctataaggc ggttatttaa agatgaaaaa aaagctattt
     7081 tcacaattat ggatctattt tgctagtatt gtcttcattt cgatcttagt gacactgctg
     7141 tgttttctcg gttttatttt cttattgtct cgacattcta tctcgcctgc tgaatcggct
     7201 ggaaaaccaa cgttataccc gttattcgtc tttgcggggt tcagtatggt cgtgggtaca
     7261 ggaatatcga tttttgttgg gcggcgtatc ctgcatccaa tcagtgcgct agggacaaat
     7321 atgagtctgg tagcaacggg agatttttcg attcgaatgg atgaacagca gaaagtagca
     7381 gaagtacagc aactgtataa agatttcaac gttatggtcc aagaactcaa tagtatcgag
     7441 acactgagga atgactttgt gtccagtgta tcccatgaat tcaaaacacc actagcaaca
     7501 atccaaggat atgttcaatt gttacaagcc ccaaatctct ctgacgaaga acgccaaata
     7561 tttttatatc ggattatcga aagtatcacg cagctttcac aattaacaga gaacatctta
     7621 aaattgaaca aactggaaaa ccagcgtatc caactggaaa agaaagagta tcgattagat
     7681 gaacaaatca gagaagtaat cgtttttttt cagcctaaat gggagaaaga acagctagaa
     7741 ttggatatcg aacttgctgc agtaaattac acaggcaatg aagagttttt gtaccaagtg
     7801 tggctgaata tcatggataa cgccataaag tacaatcaaa tcaatggaca gattcatatc
     7861 aaactatttg agacagccac agagattgta ctggaagtga ccgattcagg tgtaggaatg
     7921 aatgaagaga ccagagatcg aatgtttgaa aaattttatc aaggagatac aagtcgtcaa
     7981 atttctggga atgggctagg actttcactg gttaaaaaaa ttctagaact gcatgatgga
     8041 cgtattgatt atagcagtat cgaaggagta ggaacgacag caatgatacg gttaagtaaa
     8101 caataggaaa aagaataaaa cccctaaaaa ttctattgaa ggtttttagg ggtttttagt
     8161 cgtttttttg tgattaggga aatgttctta cggttctttt ggcataaaaa tcgctgcttt
     8221 cttattgaac tacgagaaat gttagaggtg tcattgaaac aaggacatga tgcaagaaag
     8281 agagcgaaag tcatgcgtcg ctctcttttt ttgggtggat agatggacag aaaatgtagg
     8341 gcttagtttt cttcttgatt taaatactcc caaaatgtat ctgaagccaa agaatgctca
     8401 ccgactttac gagtaaatgc aagataactc tctgttgttg gagaaatagg gagaatcaca
     8461 gtattttctt ttaatagatt cttgactgtt ttttccataa caatagcaat ccccatgtca
     8521 tttgcaatca tattgatgat caagtcgatt cttgctcctt tataagagat ttttggttca
     8581 aatccagctt cgcgacacag atctagtaca ggttgataca agttcgtagt ggggccaagg
     8641 agcaaaaagt tctccttttt cagttcgctt agatccaatt tctttttact agctaaagga
     8701 tgatttttag gtagaactgc tacaaaatcg tctgtttcag taggcaacca ctcaatcgaa
     8761 cttggtgggg cctggaaact tctagcaaag tggatcgtgt cactatgttc attggcgtca
     8821 ttgtaagtta attcgacact ttccacttct ttcaactgga ctgtaaattc aggatggcgt
     8881 ttcaaaaact cggtgatttt ggaaaaacct ttataattga tcattgtcgg tatcgttaag
     8941 atatttaatg aaagattgtt gtctttcgta taggtttcaa gcacttcttg catagcatgg
     9001 tattgttcta cgacattttt tacatatggg agcaataagc ttccagcttc ggtcaattcg
     9061 atttttctat gggcgcgttc gaataatttg acgtctaatt ctgtttctaa cgaaagaatc
     9121 tgtttagaaa tatttccttg ggtggtgaat tgtcgatcag cagttttggt gaaattcatt
     9181 gtttcggcta aatcaagaaa aacttcgaat ttatggatat ccatcatatt cctcctactt
     9241 attccaataa agaatacttt aacacaaaaa atgaattggt gtaatcttct ctgtctgtta
     9301 caatctttct tgcagcgaaa gcaataatta cagaaatttt tctgattttt ttcgcttcgt
     9361 agaaaaagag aggagttttt tgatgccaac tggagttttg atcaatgtag gttccgtttt
     9421 gtttggcgga ctaattggcg gattagtagg aaataaatta agtgaacatt ttaaagcaca
     9481 gctgacgatg gtttttggtg tgtgttcgat ggggatggga atctattcca ttgcgccgat
     9541 gaaaaatatg ccggctgtta tttttgcttt agtgatagga acagcgattg gtttgattgt
     9601 tcatttaggt aatgggataa ataagggggc tgcgctgatg caagtaccta tttctaaaat
     9661 cttccctagt gaaaagcttg gtatgaccca cgatgaattt atcagcacat tagtgacggt
     9721 cattgttcta ttttgtgcaa gtggtacggg gatctatgga agtttagatt ctgggatgac
     9781 aggagacagt acgattttga tttctaaatc tgtccttgat tttttcacag cagctatttt
     9841 tgcgtgtaat ttagggtatg tcgtttctgt gatagcaatt ccccaattca ttatttttta
     9901 tattttgttc ttattggcaa aatttattta tccattgact acaccagaca tgatcttaga
     9961 ttttaaagct tgcggcggat tcttgatggt cgctaccggt tttagaatga tcaacgtgaa
    10021 aatgttccca gtagctgaca tgatacctgc aatgatcgtt atcatgccac tgagctggat
    10081 gtggacaaat tggattatgc cactgttata ataaaaatca aagagaagcc attttttgct
    10141 gactaaaaag gatcaaatct tttagtcagt ttttttgtat ttgacaaact gattttgaaa
    10201 ctatattata tgttgtataa tatagattga aataaaatat tacacttgag aatagaaggt
    10261 gacgtaatgc acatccagat tccaactgtt cttcttgatg gtactgtgct agcagcatta
    10321 agcaaggaag atatgtatgg gtatgcctta acaaaaatcg tacaagaaag tctctctgtc
    10381 agtgaatcta cactatatcc ggttttgaga agattgaaaa aagaaggata tcttgagacc
    10441 tatgatgaac cttttgaagg cagaaaccgt cgatattact cgttgacacc aatgggaagt
    10501 cgtcatttag aagaaattat tgatgaatgg attgtttttc gagattcttt agaccggtta
    10561 ttggaggaga gcacaaatga atgaatattt gaatgaagtt gtggatcatc ttcaagaaat
    10621 tcctgaagac gatcgttttg atttgatcca gtattatgag gaatattttt tagattctgg
    10681 aaaaacatta gatcagatca ttgatgaata tggtacacca aagcagtttg ccttaaaatt
    10741 aaaaatcagt tacttttcag aagaagaaca agcagaaaac gagttagtga aaaatccaaa
    10801 aagacaaatt cgcctgattt ggttaatcat tatcggttta tttgcttctc ctgtcctcat
    10861 tccactagca ctggggttta tattggttat tgggggacta ttggttgctt tactagcgct
    10921 catttttagt atctatgttt tatgtatcag tatcttaggt gttggcctat tcatgatcat
    10981 ctcaggaatc agtgtgtttg gacaatcgat tctgagtgga ttattcttta tcggtgtcgg
    11041 tctgttcgcc acaggaagcg cagtatttat ttcgccactg ttgctgaaag gaacaaaagg
    11101 gcttttttat gtcatgatga actttgttaa atggatggga cgacgtttta ttacaaaaag
    11161 agatctgcat tcagcgggcg tttgaaggag gacacataaa ttgagattga aacatttttt
    11221 gattaccggt attggtctga tgcttactgg tggcctccta gcaggtgcaa cttatacatt
    11281 aggcgctcaa aagagtctga cttgggataa tggtcctcgg ttgatcgata tggttactga
    11341 gacaaaaaaa atcaaggatg caaacaaaat cgtcatccat gcagaaaatc aaacagtttc
    11401 ggtcatacgc ggattggatt ttgagataaa aacgacttat gataagacag aaaagccaga
    11461 aataaaggag aaaaatggcg tgttgactgt gaatgcagtc aaaaaatcaa caggtgcaat
    11521 cattagttta gaagacacaa gtgcacaaat cactattgtt gtccctgcag atcatgaatt
    11581 atctgaattg acaatcgaag ggaataatag tgcaattgat atcaacgaag tatcaatcaa
    11641 aaaagtgaat actaagacac atgatggctg ggtatcgttc aatgaattta caggaggaac
    11701 aataactgcg aaagatgaat taggctcact tcgattagat gaggccaatg tggataactt
    11761 gatcgtcaca ggtgaaaaca gcaacttcca tctagaagac agtttgatca gcaaagaagg
    11821 aaaagtcatc ctaaacggtg gcaatttatc tttgcatgag atggaaaaca gtggctatac
    11881 tatccaaacg tctggtgaat cttatattga aaaagactac cagccaataa caaatacttt
    11941 gttagaacaa ggacagaaaa agctaaagat cactgctaaa gacacatcga tcaatattac
    12001 gacggatcaa gaggacaatg aaacagattg atcctgtact caactggata ttttaagaaa
    12061 aaacagcaaa gatccttcca atctttgctg tttttcatcg cttaacgcac ataactatag
    12121 taaaataaga aagaaaatat ctaaaaagaa aacagggtga aagagtatat gaaggtagaa
    12181 gaaggtcaaa gagaaaagtt aaaaacagaa atggaaagat tacatactta tatcactcaa
    12241 ctaagccaaa ccttctatga tccagacaaa gaaaaagtga tggttaatta cccgaacaat
    12301 tcagaaggtc gccaattaga gcaagtgtac catgaagttt tcaaacattt attgacggtg
    12361 aagaaagagc tggattatta ttctttaccg attatcgata caggaatatt gaagtatgat
    12421 gggaagaagg aacgatttat ctttaaatct gttcgggaag atttgcctct ttcagcaggg
    12481 atggatctag aggtcttggt agaagactat tttacagaag aaaagcactg ggttcgaaca
    12541 aaattggatt atttaccgca ggcagccgga gggacacagg caagcggttg gtacatcact
    12601 gaagacaaag agttagaact agaaggtgta atggcgcgta ttcgaaaaaa aaattaaaaa
    12661 agtaacatta gatatttatt gaaggataaa ggagcgcagg aagtagcatg agagaaaaaa
    12721 gagcatttta tatcaatggt tatgcaggga ttgtcggatt ggtcgtatta gctttagttg
    12781 gattgttttt cttctattta ggtatgtggc aagcgaaagt ttgggcactt tttttaagta
    12841 tttttctttg gttgatcacg ttattgttgt taagttcggc aactgtagtc agtcctaatc
    12901 aagcaaaagt aattttgttt tttggacaat atttaggtac gattcgagaa aatggctttt
    12961 tccttacgat acctttggca caaaaaatga ctgtttcgtt gaaagttcgg aatttcaata
    13021 gttctgtttt aaaagtcaat gatttggatg gaaaccccat cgaaatctca gcagtagtag
    13081 tctttaaagt cgttgataca gcaaaggcat tattcgatgt agcgtattat caagattttg
    13141 tagaaatcca aagtgagaca gccattcggc atatcgctag ccaatacccg tatgatacat
    13201 tcaatgagaa tgatttgacg cttcgaggaa acacgacggc agtgtctgac gagttgcaaa
    13261 aagagctgca agagcgttta gcagtagcag gtgtcgaagt aatcgaaaca cgcttgaacc
    13321 atttagccta tgcaacagaa atcgctagtg ccatgctcca acgtcaacaa gcaaaagcca
    13381 ttttgtctgc tcgacaaacc attgttgaag gagctgtcac aatcactcaa atggccttag
    13441 agcaaatcga agatggacaa gaaatcaatt tcaccgatga tcggaaagtc caattgatca
    13501 ataatttact tgtctcaatc attacagaca aaggaacgca acctgttatc aatacaggcg
    13561 acatgaaaga ccgtaagtga catacttttt gtaagatagg aagaaggagt ttaggacgga
    13621 ttattgtctt gaactctttt tttagttgtt ttaaagaaaa tgacaaaatt gaaagaagaa
    13681 tgatagttga ttcgaaggca gaattgttcg cttttttcta taatcagtat ataaagtaag
    13741 agagaagggg aaaaagtgaa aaaacttctg atcggtctac ttgttttcat cgttgtggta
    13801 cttgcagggg tgaaaatcgc agattacgta gttatgggtg gagaagaata ctatgtacaa
    13861 attacgacag atggtacaaa aacagtttca aaatcagact ctggacaaga aacagtcaac
    13921 tatagatata tcctgcctgg ctatacgaaa gagggtaagg aaaaagaact ggacttcaag
    13981 ggacaattga ctcgaccgct tcgtaaagga gcatatttaa aaatcacctg gaacaaaaat
    14041 aaaggtgtca cttcgtatga agaagtcgaa aaaaaagacg tgccgaaaac agcactggaa
    14101 aaactaatta aagggtaggg aaagaaatga acaaaatagt cgaagtacag aatgtaacaa
    14161 aagtatacgg aaaaaacaat gagaaaaaga cgcaagcatt aagcggcatc agcttcgatg
    14221 tagaaaaagg cgaatttatt gggatcatgg gcgcttctgg ttctggtaag tcgacacttt
    14281 tgaatatttt atccacatta gataaaccaa cagatgggca tatccgaatc aatcaaaaag
    14341 atgtgaccac attaaaagga aatcaactag cagattttcg tgcgaatgag attggcttta
    14401 ttttccaaga tttcaattta ttagaaagct tgacagcaca agaaaatatc gctgttcctc
    14461 tttctttaca aggcgtcaga ccaaaagaaa tcaagcaacg tgtccaaaaa attgcagaac
    14521 gtttatccat ctctcatatt ttggaaagct atccttctga gatttccggt ggacaaaaac
    14581 aacgggtcgc cgccgcacgt gcattgatca cacaaccaac gattctatta ggggatgagc
    14641 ctacaggtgc gctggattcc aaaagtgccc gggatctttt agatacgatg gacgaattga
    14701 atacaaaaga tcatgtatcg attcttcttg tgacgcatga tccgttttct gcaagttatt
    14761 gccaacgtat tttattcatc aaggatggcg gtatccatca agaagtgaaa cgtggagatc
    14821 aaagccgtga ttccttctat cgagaaatac tatctattct tggaaacttg gaacagtagg
    14881 gggagattga ctatgctttg gaaattatca cttactggga tcaagagccg tttaagagac
    14941 tacttggtgt tattctcagg attagttatg gcatctgcta ttttttacat gtttgaatca
    15001 atggcaagca acgaagcttt tttgaaaagt aatacgatcg ttgcggctac agttatcatt
    15061 ttccgattcg gttctgtttt actagggatc atcacattcg tttatatttt atatgcaaat
    15121 tcctttttga tgacgatgcg gcaaaaagac tatgcaatgt ttatgatgct gggagcaaaa
    15181 ggcagaaaaa tcgcacagat gattttttct gaaacattct tagtagggat cgcagctact
    15241 ttagtgggtt cagctgtcgg tgtaggattg acaagtatcg tccaccgatt attagtggat
    15301 cagttagatc tcaatatttc gcattttaca ccttttagta ttcacgggtt gttgatcact
    15361 gttttgtttt ttgctgtttt gtttttatta gctgctgttg tcaatgcttt ttcgattgtc
    15421 aaaaaaccga ttttagcatt gattcgtgcg gatcagacac caacaagaat caagcagcat
    15481 aaattcttgt ttttccttga agtagtactg ggaattgctt ttctaggctg cggttactat
    15541 atgatgaaac acgtggggac ctatcaagtc tttggtatag gggtagcctt agttacgatc
    15601 gtattaggaa cttatttcat tttccattct gtgatcattt tctttttatc attactaaag
    15661 aaatcagaac agatttcatt gaaaaaattg aacaatttca cattgtcaca gttaaacttt
    15721 cgtattcgtg aatatacaca aatgctctca atggtcgcta tgctgtttgc cttggcactg
    15781 ggcgctttga cggtaggatt aggttttaaa aatgaaattg cagacttgac agaaaacaat
    15841 tctggatatg atctagtgat gaataatcct cgggcagaag atcagcaaaa aatcaaagaa
    15901 ctttcaccaa cactttctgc ttcttatact caaaaagaag atgcgggaac gatttactat
    15961 aatgcaagtg aatttgcggc tcaaccattg atcatgataa aacatgaaga tggaacaccg
    16021 ccgaagatca cgaaagaaaa agttcctgct aaaaaaatga atgaactgac tgtacaagag
    16081 gaacttcgaa gctatgaatt gccggaacag caagaaaaag aaatcaaggt agtcagtcaa
    16141 tcagaatttg atcaactgaa cttgccacaa tccactttac agctagttca ggtaaaagac
    16201 ttccaagcaa acctgaaacc aatcgaaaca cttgttgctc aaaataaaat gaacaatcca
    16261 tctttgcaaa aagtagagta cagcaaccag aaatatgaaa tgtatgaagt gtataatggt
    16321 ctattctctg gttttgaatt tatgggattc ttcctaggtt tagctttctt gaccatgctt
    16381 gctagctgtt tgatgttcaa gatcttgtca ggaagcaaga gtgacattgc tcgttatgaa
    16441 atgcttgaaa agataggaac acgacgcggg ttgcttaaac aatcgatccg tagagaagta
    16501 ggcgtgttat tcttagcacc aggaatctta ggtgtagtcc atgtcttatt cggattacaa
    16561 atgtttggtc tattcatgca aaatccatac aaagatattt gggttccgtt tacgatattc
    16621 ttcgtattgt atttcatcta ctatgtactg acgacatggt tatatactgg aatcgtattg
    16681 aaaaagagat tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca agcaaaaata
    16741 gctcctcata tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg
    16801 tttgttcgga aaaagaggtt gtgacaatat ttttgtcaca accttttttt cacggtcttt
    16861 tattagaata tagtaaattt tttctcgtta ctcacggaat aagctgttaa gaaaacggtt
    16921 tccatgtata ctggaatcag tgcaaatgaa agaaaagagg gtgcaaggaa catggaaatc
    16981 aatgaacaaa attttggcga aaactatcac ttgattacga tcacgaatca aaatgggaca
    17041 tctttgtctg tcagtgatct tggggcacgg gttgttcatt ttcaaacaat aatcgataat
    17101 gaaaaacgag agttgatctt aggttttgat gaagcaaaag aatatctaga aaaagaccct
    17161 tatatcggtg cttcaatcgg acgtatggct ggacggatta cgaatggcac attcaaatta
    17221 gatgatgaaa cgttcgaagt aaaaacagac ccggacactg gtcattgttt acatggtggt
    17281 gcacctagct ttgaagccaa gcagtggcat tatacagtca tcaatggtga aaacgaagca
    17341 tcggtcattt tctatcagac tagcccagat atggaaaacg gatttcctgg aactttagat
    17401 gtagaagtac ggtatacgct gacgaacgat gatacatggc gagtgacggt ccaagggatc
    17461 agtgaccgca aaacagtatt caatcctaca aatcatgtct atttcaattt aaccggtgat
    17521 ccagcacagt caatcgacga acatatactt tggttgaata gccgattatt tgctacgctc
    17581 aataaagata caacgcctac aggagaagta gtttcagtaa gtggcacagc atttgacttc
    17641 caacgtccta aaaaattagg tgaggtcttt gccagtgatt ttcgccaatc aatgatagtg
    17701 ggcggcatcg atcatccatt tttgttgaaa aaacaatctg gtttactaaa tgctgcggaa
    17761 ttgacaagcc cagatgaaaa agtatcgatc caagtaaaaa cagatgcttc aggaatcgtg
    17821 atttttacag caaattttgg agaagatgga ccggaaatgc gcgggaataa attagtaaat
    17881 catggcggta tcacatttga gactcaagaa ctgccaggag cggagcgact tgaaagtttt
    17941 ggagatgtca ctctggaacc taaccaagta cgcgagtttg taacggaat
//
LOCUS       DAXWOO010000042        17832 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.042, whole genome shotgun sequence.
ACCESSION   DAXWOO010000042 DAXWOO010000000
VERSION     DAXWOO010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.042"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            121..933
                     /locus_tag="ACGE70_001173"
     CDS             121..933
                     /locus_tag="ACGE70_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFF8692823.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLLRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            930..1730
                     /locus_tag="ACGE70_001174"
     CDS             930..1730
                     /locus_tag="ACGE70_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFF8692824.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1891..3159
                     /gene="rseP"
                     /locus_tag="ACGE70_001175"
     CDS             1891..3159
                     /gene="rseP"
                     /locus_tag="ACGE70_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFF8692825.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3227..4936
                     /locus_tag="ACGE70_001176"
     CDS             3227..4936
                     /locus_tag="ACGE70_001176"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFF8692826.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5148..9500
                     /locus_tag="ACGE70_001177"
     CDS             5148..9500
                     /locus_tag="ACGE70_001177"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFF8692827.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9644..10117
                     /gene="rimP"
                     /locus_tag="ACGE70_001178"
     CDS             9644..10117
                     /gene="rimP"
                     /locus_tag="ACGE70_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFF8692828.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10141..11316
                     /gene="nusA"
                     /locus_tag="ACGE70_001179"
     CDS             10141..11316
                     /gene="nusA"
                     /locus_tag="ACGE70_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFF8692829.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11338..11631
                     /locus_tag="ACGE70_001180"
     CDS             11338..11631
                     /locus_tag="ACGE70_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFF8692830.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11628..11939
                     /locus_tag="ACGE70_001181"
     CDS             11628..11939
                     /locus_tag="ACGE70_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFF8692831.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11952..14258
                     /gene="infB"
                     /locus_tag="ACGE70_001182"
     CDS             11952..14258
                     /gene="infB"
                     /locus_tag="ACGE70_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFF8692832.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14285..14632
                     /gene="rbfA"
                     /locus_tag="ACGE70_001183"
     CDS             14285..14632
                     /gene="rbfA"
                     /locus_tag="ACGE70_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFF8692833.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14740..15244)
                     /locus_tag="ACGE70_001184"
                     /pseudo
     CDS             complement(14740..15244)
                     /locus_tag="ACGE70_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            15409..17772
                     /locus_tag="ACGE70_001185"
     CDS             15409..17772
                     /locus_tag="ACGE70_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
                     /protein_id="HFF8692834.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
BASE COUNT         6095 a         3153 c         3920 g         4664 t
ORIGIN      
        1 aaaaaaagaa aaaattattc agtttttaaa taaaaaaaca atgatttcat aggaaaaaca
       61 gttagatatt gctataatac ttaagatagt gggaatcttt taagtatagg aggaaaaaga
      121 atgttacgtt tttttccgca aaaaaacaaa tatattcaag aaaaaagcga atttcaattt
      181 aataccgatg gaccaattcc tcagcacatt gccatcatca tggacggcaa tggtcgttgg
      241 gcgcaaaacc gtcgtttgct acgagttgct ggccataaag aaggcatgga aacagtaaag
      301 aaagtcacaa aaaaagcatc aaggcttgga gtgaaagtgt tgacgcttta tgccttttcg
      361 actgaaaact ggaagcgacc aaaagatgaa gtaagttttc tcatgcaact acctgtcgac
      421 ttctttgata cgttcgtacc agaattgatt aaagaaaatg tcaaagttca tgtcatgggt
      481 tatgaaaacg tattgccaga acatacgcaa gatgctgtaa gacgtgcaat tgagcaaaca
      541 aaaaacaata ctggaatggt attgaatttt gctttgaatt atggaagtag agcagagatc
      601 gtgactgctg tccaagaaat tgccgaagaa gtagcaaaag gagaaataca cgcagaagaa
      661 atcgatgatg aattgattgc taagcattta atgacaggtt tcttgcctaa agagttacaa
      721 gatcctgagc tgatgatccg tacaagtgga gaagagcgaa ttagtaattt tctgttatgg
      781 cagatcgcat atagtgagct gtattttaca aaagcgcttt ggcctgattt tgatggtgcg
      841 catcttgaag aagcaatcgc ctcttatcaa aatcgagacc gtcgattcgg cggtgtaaaa
      901 aaaacagaag atgagaaagg ggatcagtca tgaaacaacg tgtgatcaca gcagctgtgg
      961 cgctagtctt gtttattccg attatatgga tcggaggcat agcggttgag cttacagcag
     1021 caatcttagc ggtagtagga gtttacgagc tgttccggat gaaaggattg acactcttaa
     1081 gttttgaagg agttttatcc gccgttggtg cagtgttact tgttttgcca aaagaaagat
     1141 ggtttttctt tcttccagag aatgtaagta catttatgtt attttatctt atagttatga
     1201 ttcttttagg aacatcagtt atttctaaaa atacgtatac gattgatgaa gcaggttttc
     1261 ctgtgcttgt cagcttgtat gtgggaatag gtttccagaa ttttgtcaat gcacgaacag
     1321 aaagcttggc tgttttgata tttggtctat ttattgtttg ggcaactgat atcggagcat
     1381 atatggttgg tcgacgttat ggtcagcata agttaagtcc tgatatttcg cccaacaaaa
     1441 cgattgaagg tgcgctcggt ggtatcggta gtgctgttgt agtcgcattt ctttatttat
     1501 tgctataccc agcaaaagaa ctattcaatc atggtatgtt tgtcatgctt ctgatgacga
     1561 ttattttttc agcagtcgga caatttggtg atttggtcga atctgcaatc aaacgtcatt
     1621 atgatgtgaa agactcggga aatatcttac caggccatgg tggaatctta gaccgtttcg
     1681 acagtttgct gtttgtattt cctatcatgc atttatttgg cattatttag taagtatacc
     1741 tgcgtttctt tgtgaatttc ataaaagacg caggttttta tttttcatta tattgatact
     1801 attggtgtct ttggtatact atggtaaaat tatagtgttt tatcgttgtt ttataaaaaa
     1861 cacaaaaaac aagtaaagga gaaaaaagct atgaaaacga ttctgacatt tatcatcgtt
     1921 tttggtatat tagtgattgt tcatgagttt ggtcatttct tctttgcgaa acgatcagga
     1981 atcctcgtcc gcgaatttgc tatcggtatg ggtccgaaaa tttatgggca tcaagcaaaa
     2041 gacgggacaa catatacgct tcgcctatta cctattggcg gttatgttcg aatggcaggc
     2101 aatggggatg atgaaacaga aatggcacca ggaatgcctc tttctctttt actaaattct
     2161 gacggcatcg tagagaagat aaatttaagt aagaaaatcc aattgacaaa tgctatcccg
     2221 atggaattga gccgttatga tttagaagat gagctgacta tcactggtta tgtgaatgga
     2281 gacgaaacag aagtagtaac ctaccctgtt gatcacgatg cgacgattat tgaaaacgac
     2341 ggaacagaaa tcagaattgc gccaaaagac gtacagttcc aatcggctaa attgtggcag
     2401 cgtatgctga ccaactttgc tggacccatg aataacttta ttttggcaat cgtgttattc
     2461 atcattctgg catttatgca aggtggtgtt caagtgacga acactaatcg cgtaggagag
     2521 atcatgccta atggagctgc ggcagaagca ggcttaaaag aaaatgatga ggtagtcagt
     2581 gtggacggaa aagaaatcca ttcatggaat gatctgacaa cagtcattac gaaaaatcca
     2641 ggtaaaacat tagactttaa gatcgaacga gaaggtcagg tgcaatctgt cgacgtgaca
     2701 ccgaaatctg tagagtcaaa tggcgaaaaa gttggccaac ttggtataaa agccccaatg
     2761 aatacaggtt ttatggataa gatcattgga gggacgcgtc aagctttcag cggttcattg
     2821 gaaattttca aagccttagg atcactattt acaggtttca gtttagataa attaggcgga
     2881 cctgtcatga tgtaccaatt gtcttcagaa gcagcaaatc aaggtgtaac aactgttatt
     2941 agtttgatgg cgcttctttc aatgaatctc ggaatcgtca atctgcttcc gattcctgcc
     3001 ttagatggcg ggaaattagt cttgaatatt tttgaaggga ttcgcgggaa accattaagt
     3061 caagaaaaag aaggaatatt gacattagct ggctttggat ttttaatgtt gttgatggtg
     3121 ctagttacat ggaacgatat tcaacgattc tttttctaga aaaagttgac aaatagggta
     3181 taataacaaa cgataactat tttgaggaaa taaaggagta tatagaatga gacaatcaaa
     3241 aatgttgatt ccaacattac gcgaagttcc aaatgatgca gaagtattaa gccatcaaat
     3301 cttgctaaga gctggatata tccgccaagt atcagcgggt atttattcat atctgccatt
     3361 agcaaatcgt gtattggaaa aactgaaacg cattatgcgg gaagaatttg aaaaaatcgg
     3421 agcggtagaa atgctgatgc ctgccatctt gccagcagaa ctttgggaag aatccgggcg
     3481 ttacgaaaca tatggaccaa acttgtatcg tttcaaagac cgcaatgatc gaaaaatgat
     3541 ccttggacca actcacgaag aaacttttac tgatttgatg cgtaatgaaa tcaattcata
     3601 taagaaatta cctttaaatc tttatcaaat ccaacctaaa tatcgtgacg aaaaacgacc
     3661 tcgcttcggt ttgcttcgcg gaagagagtt tatcatgaaa gatgcatatt ctttccatgc
     3721 aacggaagaa agtttggacg agacatataa agattacgaa aaagcctata ccgaagtatt
     3781 caagcgttgt ggcttagaat ttcgctcgat catcggagac ggcggcgcaa tgggcgggaa
     3841 agactcaaag gaattcatgg cgatctcaga aatcggggag gatacgatct gttattcgac
     3901 tgaaagtgat tatgctgcaa acttggagat ggctacaagc tactatgtat caaaaaaatc
     3961 gcatgaaaca cagcttgaat tggagaaagt agctacacct aacgtaaaat caattgacga
     4021 agtagcagat ttcttttctg ttgaacctca aaaaatcatc aagtctgtat tgttcattgc
     4081 ggatgaacaa cctgtccttg ttttggtacg tggagaccat gaagtcaatg acgtaaaact
     4141 gaaaaacttt ttatctgctg actttttaga agaagcaaca gaagaagaag cacaaaaata
     4201 tctaggtgct gagtttggtt ctgtcggacc agtcggtgtt tcagatgacg taaaagtata
     4261 tgcagaccgt tatgtccaag atttagcgaa tgccattact ggagcgaacg aaacaggatt
     4321 ccactataaa aatgtgaatc caaaacgcga tttcactgtg ctttcttatg aagatcttcg
     4381 ttttgtacaa gaaggagatc cttcaccaga caacaacggt gttcttgcct ttactcgagg
     4441 aatcgaaatc ggacatatat tcaaaatcgg cactcgttac agtgaatcta tgggagcaac
     4501 tgtactagac gaaaatggac gagaaaaatt tgtgattatg ggctgttatg gtatcggtgt
     4561 aagtcgttta ttatcagcaa tcgtagaaca acattcagat gaacaaggaa tcaattggcc
     4621 aaaaggaatt gcgccatttg atctgcatat cgtccaaatg aatctaaaag acgaatacca
     4681 aacaagcctg acgaatgaac tagaagaagc gatgacaaaa gctggctatg aagtactggt
     4741 tgatgacaga aatgaacgcg caggagtgaa attcgcggat tctgatttga ttggctgtcc
     4801 aatcagaatc actgtcggta aaaaagcagt ggatggaatc gtagaagtga aaatcaagaa
     4861 aactggcgaa atggttgaag tacgtaaaga agaattggcg aatactttac caatcctctt
     4921 gaaccaaggc gaataataag cgttaaaaca cagaaaataa aaatagctga atcagctgtt
     4981 tttgacaatc tgatgattcg cttttaaaat tggaactgcg atttgaatcg cagttccaat
     5041 tttttaatgt aaaactcaga aaaaacgagt atactaaaag gtactgaaaa agaagaggat
     5101 aagatgcgat caattgcatt gtttctcatc agaaagggcg tatatctttg tcagaaaaac
     5161 gagatttatt tgaaaaactg ttggaacaaa tccaactaga ggaagcagaa aaaaatcatc
     5221 cactgcttac tgctggtgaa atggatagtg tagtggtcca tcgtaaatcg cgtttatggg
     5281 aatttacgct acatttttcc aaaattttgc ctattatgct atatcggtca ttgactcagc
     5341 atctgactat cgcttttaaa gatattgcac aagtcaaact gaatatctta gcagatgacc
     5401 agacattcga tgaacagctc ttgcaagatt actgggccca agctttggaa aatcagcaat
     5461 gcgatacacc actagcacag caagtattga aaacgcaggt acctgtgata aaagatcgta
     5521 aagtcatctt gccaatcgac agtacaggag ctatttctta tttgaagcaa caatacttgc
     5581 cattagtaga agagttgttc gtcagttacg gattcccaaa atttcgtatc gaaccagaag
     5641 tggatgagca gcaagcggaa cgtgtattga aactttttga agaacgcaaa caagaacagg
     5701 cagaagcctt tatgaagcaa gcagctgaaa gtctagttgt ccacgaacaa aagaaaaagg
     5761 aaagaaaaga gcaatcgcca gctcttgaag gacccatcca gctaggacga aatattccag
     5821 ccgatgaacc aacgacacca atgatcagta tcgttgaaga agaacgaaga gtcacacttg
     5881 aggggtacgt attcgacaaa gaagtcagag aactacgttc aaaacgcaag atcttgactt
     5941 tgaaaatcac agattatact tcatcattta tcgtcaaaaa attctcgaac ggcgaaaaag
     6001 atgaacaagt atttgatgcg atcagtgtcg gtagctggtt aaaggtacga ggaagtattc
     6061 aagaagatac gtttgtccgt gatttggtca tgaatgcgca agatatcata gaagtcaaac
     6121 atacacctcg caaagattat gctcctgaag gggaaaaacg agtagagctt catgttcata
     6181 gcaatatgag tacgatggat gcaacaaaca gcatctctga tcttgtggca caagcaggaa
     6241 aatggggaca ccgagctatc gccattacag accatggtgg tgcgcaagcc tttccggaag
     6301 cacatagtgc tggaaaaaaa gcaggtgtga aaatcttata cggcgtggaa gcgaatgtcg
     6361 tagatgatgg tgtcccaatt gcttataatg atgcacatga agcattgagt gaagccacat
     6421 acgtcgtatt tgacgtggaa acgacaggat tgtctgcagt atatgacacg atcatcgagc
     6481 tagccgcagt caaaatgtat aaaggaaatg tgatcgaaag ctttgatgaa tttatcgatc
     6541 caggacatcc attgtcacga acaacggtag atctgactgg tatcacggat gaaatggttc
     6601 gagggtcaaa gtcggaagaa gaagtattgc ggatgttcct tgaattttca aaagatacaa
     6661 ttttggtagc ccacaacgcg gcttttgata tggggttctt gaatactagc tacgcaaggt
     6721 atggtattcc tgaagcggcc aatccagtca ttgatacgtt ggaattagct agatatctgt
     6781 atcctcaatt caaacgtttc ggattaggtg tgttatctaa aaagtttggt gttagcttgg
     6841 aacagcatca ccgagcaatc tatgatgctg aagcaactgg tcacttagca tggatctttg
     6901 tgaaagaggc aatggacaat cataacatgc tttatcatga tcagctaaat gagcatatcg
     6961 gagaaggaga ttcttacaaa agggctcgac ctttccatgt aacgatttta gcaaaaaatc
     7021 aggcaggact gaaagatttg ttcaaactga tttcaatgtc gaatgtcgaa tacttcgaac
     7081 gcgtcccacg aataccacgc tcccaattga aaaagatgcg tgaaaatcta ttgatcggct
     7141 cagcttgtga taagggagaa atattcgaag ccatgatgca aaaaggggta gaagaggcaa
     7201 gaaaccgagc aaaattctat gattatattg aagttatgcc aaaagcggtc tatgcgcctt
     7261 tgatcgagca agaattggta aaaaatgaac atgatttgga agaaattatc caaaatttgg
     7321 tagaaatcgg aaaaagtttg gataaaatcg ttgttgcaac aggtaatgta cattatctga
     7381 acgaagaaga tgccatttac cgaaaaattt tgatcaattc tatgggcgga gcgaatccgt
     7441 tgaatcgtca tagtttgccg gatgtccatt tccgaacaac cgatgagatg ctaactgcat
     7501 ttcacttttt aggggaagaa acagcgaaag agatcgtagt agaaaatacg aataaaatcg
     7561 cagatatctg cgaagaagtg attccagtca aagatgaact ttatacaccg aagattccag
     7621 gttcagaaga tgaaatatca gaattgagtt acacaaaagc aaaacaaatg tacggagatc
     7681 cgttacctga gatcattcaa aaacgattag aaaaagagtt gaattctatt aatggtaatg
     7741 gcttctcggt tatttatctg atcgcccaaa aactagtgca taaaagtaat gaagatggct
     7801 atctagtagg ttcacgtggc tctgttggct ctagctttgt tgcgacgatg acaggaatca
     7861 ctgaagttaa tccattagcc cctcattatt attgtccaga gtgccaatat tccgagtttt
     7921 ttgaagatgg tacgtatggt tctggatttg acatgccaga aaaacaatgt ccaaaatgtg
     7981 gtgcacgctt aaacaaagac ggtcatgata ttccatttga gacattttta ggattccatg
     8041 gagacaaagt gcccgatatt gatttgaact tctcaggtga ttatcaagca gaagcccata
     8101 actatacgaa agtattgttt ggcgaagact atgtttaccg tgccggaacg attggtacag
     8161 tcgccgacaa aacagcatat ggctatgtaa aaggatacga aagggacaat aatctgcagt
     8221 ttcgaagtgc agaagtcgac agattagcaa aaggcgctac tggagtcaaa cggacaaccg
     8281 gccagcatcc aggggggatc atcgttattc ccgattatat ggatgtctat gattttacgc
     8341 cgatccaata tccagcagac gatcaaaatt cagaatggaa aacgactcat tttgatttcc
     8401 actcgattca cgataatgta ttgaaattag atattctagg gcatgatgac ccaactgtta
     8461 ttcggatgct tcaagattta tcagggatcg acccacaaac gatccctacg gatgacccgg
     8521 aagtgatgcg gatatttgcc ggtcctgaag tacttggagt aagtcaggaa caaatttatt
     8581 ccaaaacagg gacattaggt attccagaat tcggcactcg ttttgttcga gggatgttag
     8641 aagaaacgca tcctacaact tttgcagaac tactgcaaat ttccggacta tctcatggta
     8701 cagacgtatg gttaggaaat gcggaagaat tgattcgacg aggagatgcg acactggcgg
     8761 aagtaatcgg ctgtcgtgat gatattatgg tttatttgat ccatgctgga ttagatagcg
     8821 ggatggcatt taaaatcatg gaaactgttc gtaaaggaca atggaacaag atccctgatg
     8881 aattgcgaga aacctatcta tcggcgatga aagaaaataa tgtgcccgac tggtatatcg
     8941 attcttgttc aaaaatcaaa tatatgtttc cgaaagccca tgctgcagct tacgtattga
     9001 tggctcttcg tgtcgcttat ttcaaggttt atttccctat tttgtattac tgtgcatact
     9061 tttctgtacg tgcagacgac tttgatctag tctccatgtg caaaggaaaa gatgctgtga
     9121 aacaagcaat gaaagaaatc acggacaagg gattagatgc ttccgtaaaa gaaaagaatc
     9181 aactgacagt gctggagtta gccaatgaaa tgctggaacg aggattcaaa tttggaatga
     9241 ttgatttgta taaatcggat gccgttaact ttgttattga aggtgatacg ttgatcgcac
     9301 catttagagc tgtcccaagt ttgggaacca acgtggcgaa acaaatcgtt gaagcaagaa
     9361 aagacggtcc atttttatcc aaagaagatc ttgccacccg cggtaaagtt tcaaaaacat
     9421 tgatcgaata tatgaacgac aatggcgtat tgaaagatct accagatgaa aatcaattat
     9481 cgttattcga tatgctataa gcatctgttt tcctgtctgc ttgtaatttt aagcagagta
     9541 tggtatagtt cttataggta atgatactaa ctgtgagtga gcggattttt cgctcactct
     9601 ttttaatgga attacgcact taatttttaa ggaggcgaaa cttttgagta gcgtcgttga
     9661 aacagtcaca gaaatggtga caccgatttt agatgaacaa aattttgaac ttgtcgaagt
     9721 cgaatttgta aaagaaggaa aaagctggtt cttacgagta tttattgaca aagaaggcgg
     9781 tatcgatatc gaagaatgcg cctttgtcag tgagaaactg agtgaaaaac ttgatgcagc
     9841 agatccagat ccgattcctc aagcttattt cctggaggta tcctccccgg gagcagagcg
     9901 gccattgaaa aaagaaagcg actatgaaaa ggcacttggt gattatgtcc atgtctcctt
     9961 ataccaacca gtagatggcg aaaaacaata tgaaggattt cttcaatcat ttgatgctga
    10021 acagcttaca ttgaaaatac gcataaaaac acgagaaaaa gaattgactt ttgaccgaaa
    10081 aaatattgca aaagctcgct tagctatcca attttaatca cggagaggaa tcagaaaaaa
    10141 atgagtaaag aaatgttaaa tgcgttagac gctttagaag ctgaaaaagg gatctcaaaa
    10201 gaaatcgtga tcgatgcttt agaagcagca ttggtttctg catacaaacg tcactatggc
    10261 caagcacaaa acgtcgaagt ggaatttgat cagaaaaaag gaaagatcca tgtctatgcg
    10321 gtaaaagaag taacggaaga agtgatggat tcacagttgg aagtctcatt gaaagatgca
    10381 cttttaatca atccagcata tgagatcggt gatacgatcc gttttgaagt aacaccaaaa
    10441 gattttggac ggatcgctgc gcaaacggct aaacaagtta ttttacagcg tgtacgtgaa
    10501 gcagaaagaa cgattattta taacgaattc agtgcttatg aaaaagacat catgcaagga
    10561 atcgtcgaac gccaggataa acgttatatt tatgtcaatc ttggtaaaat tgaggcagtt
    10621 ctttctaaac aagatcaaat gccaaatgaa ttttatcaac cacatgatcg aatcaaagtt
    10681 tatgtttccc gagtagaaaa tacgtctaaa ggtccgcaag tatttgttag ccgcagccat
    10741 ccagacctat tgcgtcgctt atttgaacaa gaagtaccag aagtctatga tggaatcgtt
    10801 gaaattgtca gcatcgcccg tgaagctggc gatcgttcaa aagtggcagt tcgctcaaat
    10861 gatccaaata tcgatgcagt cggtacatgt gtcgggccaa agggacaacg agttcaagca
    10921 atcgtcaatg aattaaaagg cgaaaacatg gatatcgtcg aatggaatga agatccagct
    10981 gtattcatcg caaatgcatt gaatcctgca caagtcatgg atgtgatctt cgatgagaca
    11041 aatccaaagg cctgcacagt tgtcgttcca gactaccaat tatcgctagc aatcggtaaa
    11101 cgcggacaaa atgcacgttt agctgctaaa ttgactaacc ataaaatcga tatcaaatca
    11161 gaatctgaca tgacggaatt ttatgaaaat caagaacaac aaaaagaaac agaagaactg
    11221 catgatgaag caatcgttca gtcagattta acagacgatg aatatgaaac aattgcattc
    11281 aacaatgaaa cggtcgaaga aaaaccagaa gcttaacttt agtaaggagg caaagagatg
    11341 aaacaaagga aaattccttt acgcaaatcc gtggtttcag gtgaaatgaa acctaaaaaa
    11401 gagatggtcc gaatcacccg ttcaaaagaa ggggaagtat cgatcgatcc aactggaaaa
    11461 atgcctggac gaggagcgta tgtctcactt gagccggaag aagtacaaca agcttgggat
    11521 aaaaaaattc tcgatcgagt actcgaaaca acattaacag atgagtttta tcaagaactt
    11581 cttgactatg tcactcacca aaaagcacgt aaagagttat ttggaaaatg aatacagtta
    11641 atcggcaaaa agccatgaat ctcatcggtc tagcgatgcg cgcgggaaaa ctaatcacgg
    11701 gtgaagaact gaccattgct gaaatccgca aacaaaaagc aaaattggtg tttgtggcaa
    11761 ccgatgcaag tgagaataca agaaaaaaga taaaagataa gagttcatat tatgaagttc
    11821 cttgctttga gctattttct gaagcagaaa tcacgcagat gatcggaaaa cctcgcaagg
    11881 tattcggaat aatggatgcc ggatttgcga agaagaccaa ggagctaatt gaaggttagg
    11941 aaggtgattg catgggcaac aaaagaatct atgaattagc aaaagaactc aaaaaatcta
    12001 gtaaagacgt cgtggaaaaa gcacaacaac ttggcatcga tgtgaaaaac catatgggtg
    12061 cgattactag cagtgatgag aagaaattgc aacaagcgtt caaaaaccca agcacgaaac
    12121 aacaagctgc acaacaagca agcagaaaac cagccaatca acagccgaac gatcaacgta
    12181 aaaaagaaac aacgaacaat caacaaaaaa ataaaaataa ccgcagttat caagatcgcg
    12241 gtcaagggag cggtcaagtg aatcaaggaa aaaaaccaac aacaaaccaa aataatacgg
    12301 gaaataccag cagccaaaat cgtcaaggaa cgagccaagg cggcaatcaa aaccgtacta
    12361 acaataacaa caaaccaaac aataacaatc gcggcaaatt taataataat aaccgtaatc
    12421 gttataataa aaaaggtaaa aaaggcaaac agcaacaatc aactaaacca gctgtaccac
    12481 cacgcaaatt ccgtgaattg ccagatgtat tagagtatac tgaaggtatg aacgtagcag
    12541 atattgcgaa aaaaattcat cgtgagccag cagaaatcat taaaaaatta tttatgcttg
    12601 gtgtcatggt caatcaaaac caagcactgg ataaagatac gattgaactg ttagcgacgg
    12661 actatggcat ggaaccacaa gaaaaagtcc aagttgacat tgctgatatc gataaattct
    12721 tcgaggcaga agaagtcgat ccagataaat tagtctcacg tccgccagta gtaacgatta
    12781 tgggacacgt agaccacggg aaaacaacct tattggatac attgcgccat tcacgtgtaa
    12841 caagcggcga agccggcgga atcacacagc atatcggtgc ctaccagatc gatattgatg
    12901 gtaaaccaat cactttcttg gatacaccag gacatgcggc ctttacaagt atgcgtgcac
    12961 gtggagcaag tatcacagat atcacgatct tagttgttgc tgcagatgat ggcgtcatgc
    13021 cacaaacagt tgaagcaatc aaccatgcca aagctgcagg cgttccaatc atcgtcgctg
    13081 taaataaaat tgataaacca ggtgcaaatc ctcagcatgt tatgcaagaa ttaagtgagt
    13141 atgaattaat tcctgaagca tggggtggag aaacgatttt tgttgagatc tcagcgaaat
    13201 tcggccaaaa catcgaagaa ttgctggaaa tgatcctgtt agtcgcagaa gtagaagatt
    13261 taaaagcaga tccaacacaa cgcgcaatcg gaacagtgat cgaagcacgt cttgataaag
    13321 gtaaaggtcc tgtgtcaacc ttacttgtcc aagaaggaac acttcacgtt ggtgacccaa
    13381 tcgttgtcgg taatacttac ggacgtgtcc gtgtaatgac aaacgatctt ggccgtcgcg
    13441 ataaagaagc aggacctgca acgccagtag aaatcactgg cttgaatgat gtacctcaag
    13501 caggtgaccg tttcgtagtc ttcgaagacg aaaagacagc acgtgcagcg ggtgaagaac
    13561 gcgcaaaacg tgcattattg gaaaaccgtg ctgccacaag ccgtgtgact ttggacaact
    13621 tatttgaaag cctgaaagaa ggcgaattga aagaagttaa cgtaatcatc aaagcagatg
    13681 tacaaggttc agcagaagca ctggctgcat cattgaagaa gattgatgtg gaaggtgtcc
    13741 gtgtaaaaat cgtccattca gctgtcggag caatcaatga aagtgatgta accttagcag
    13801 cagccagcaa cgctattatc atcggtttca acgttcgtcc tacaccgcag gcaaaagtgc
    13861 aagctgatac tgaagaagtc gatattcgtc ttcaccgtat catctataaa gccatcgaag
    13921 aaatcgaaac tgcgatgaaa gggatgctag accctgaatt tgaagaaaaa atcaccggtc
    13981 aaatgatcgt tcgtgaaacc ttcaaagttt ctaaagttgg tacgattgca ggtgcctatg
    14041 taacagaagg atatattcgt agagatagcg gtgtccgtgt aatccgtgac ggaatcgtaa
    14101 tctatgaagg ccaacttgca agtctgaaac gattcaaaga tgacgtgaaa gaagtcaaaa
    14161 tgggttatga atgtggtgcg atgattgaaa aattcaatga catcaaagta gatgacgtaa
    14221 ttgaaggatt tatcatggaa gaagtcaaaa atgactaaaa actgataaaa ggaggccaat
    14281 cattatggct aactaccgtg atcgccgagt cggtcaagaa atcttaaaag aagtcaatga
    14341 tattttgcgc aaaaaggtcc gtgaccctcg tgtggagaac gtaacgatca cagatgtgaa
    14401 agttacgggg gacttacagc aagcaacgat ctattacagc attttatctg acttagcatc
    14461 agataagaaa aaagcacaag atggattgaa taaagcatca ggattgattc gtcgagagtt
    14521 agggcacaat ctaagtattt ataagacacc tgaattgttc tttgaacttg atgaatcagt
    14581 tgtctatggg aaccatatcg atgaattgct tcgcgacttg aacaaagact aatcaaaaaa
    14641 tcccgcaaat cctgtgaaat caggggttgc gggatttttt tgttttcatt aagacgtatt
    14701 acctaagagt tccatgactt tagtctggta gcgtgtcagc taaccaaaaa agagaaaaag
    14761 aaaagtaaaa aattcgactt tcttttttct atagtatata aatttttgca tatagataaa
    14821 gatcaagata ccgataacgg aagctagatt gtaatctatt ttagtgacag tcaggtatag
    14881 ccaagtaact agaagatcag tggaacctgc accataaaag tttgagtttg tcggtccgtt
    14941 gacagttaaa agaaaaatca cgttgaagtt attgatattt ccgattacgc cgcttcgatt
    15001 tggtcacgat ccaaattttg gataatccct gtagaaatta gcatagtagc tgggatacca
    15061 attcacatat tgacaaagat aacagagact tttacataaa acggatcaat caggaaagga
    15121 attgctcgat ccagccccag ttttccaaca atgtgttaac tggcctagcg tcattcagca
    15181 aattatgcat gatgaggagc gaaatgaact gcggaacagc cattgtgata acgacaagtt
    15241 ttttggatca atgtaaaaaa cgtcagcgaa aggaaaaatt tcgtggtttt ttatacctgt
    15301 tcatttggct agatataaaa tccaaacaaa tgaacagaag tgcttattag gtttcaattg
    15361 ggagtatctg cgctagaata agagtaataa tgaaaaggag tgttatgtat ggattattct
    15421 agtaaagaat actttgataa aatgacggct tggtggcgtg ctgctaatta tctttctgtt
    15481 gggcaacttt atcttaaaga taacccctta ctgagacgta cactgaaacc agaagatgtc
    15541 aaaaaacatc caatcggtca ttggggaacg attcctggcc aaaattttat ttatgtccat
    15601 ttgaaccgag tcatcaataa atatgatttg aacatgtttt atatcgaagg gccaggacac
    15661 ggcggacaag tcatggtatc gaatgcttat ttagatggaa gttacacaga gatctatcca
    15721 gaagtaacgg aagatgagac aggaatgcaa aaattattca agcgattttc atttcctgga
    15781 ggcattgctt ctcatgcagc accagaaaca ccaggttcta tccatgaagg cggagagctt
    15841 ggatactctc tttcacatgc agtcggagct gtattagaca atcctgaagt aatctctgct
    15901 gtcgtgattg gtgatggtga agcagaaacc ggtcctttag ctggaagctg gttctcaaat
    15961 gtctttatca atccagtgat agatggagct gttttaccga tccttcattt aaatggtgca
    16021 aaaatagcca acccaacgat cttggcacgt aaatcagatg gagaattagc aaattacttt
    16081 aacggacttg gctgggagcc tttctttatt gaaggaaatg atccagaaaa attaaatcct
    16141 gttatggctg aaaaaatgga tcaagcaatc gagaaaatca aatccatcca aaaagaagct
    16201 cgcctgaaga cagcaacaga tgtcgtgatg ccaaaatggc cagtattgat cgtacgcaca
    16261 ccaaaaggat ggaccggtcc aaaagaatgg gacggggaac caattgaagg aaccttccga
    16321 gcacaccaag taccgattcc tgtagatcaa gaacatatgg atcatgcaga tgctttactc
    16381 cgttggctga aatcctatga accagaaaaa ttatttgatg cacaaggacg tattttagaa
    16441 gaaatcagag aaattgcgcc tactggtgac caaagaatgg caaaaaatcc tatcaccaat
    16501 ggtggaatcg atccaagacc gcttattatg ccagattgga aaaaatatac tttgcaattt
    16561 gaaaaaccag gctccgttaa agcagaagat atgaccgaac ttggtaaatt cgttcgcgag
    16621 atcattgaga aaaatccaga aaatttccgt atatttggac cagatgaaac aaaatcaaac
    16681 cgattgaatc aagttttcaa aacgacaaat cgccaatgga tggaaaaaat cgaaccagaa
    16741 aatgatgaat ggctttcgcc atctggtcgc gtcatcgaca gtcaattatc ggaacaccaa
    16801 gatgaagggt tcttagaagg gtacgttctg actggacggc atggtttttt tgcaagttac
    16861 gaatcattct tgcgagtggt tgactctatg ttgactcagc acttcaaatg gatacggaaa
    16921 tcacatgacc tttcttggcg aaaagattat ccttcgctga atctcattgc aagctcaaca
    16981 gtcttccagc aagatcacaa tggatatagc caccaagatc caggaatatt gacacatttg
    17041 gcagaaaaga aggcggagtt catccgtgaa tatctgcctg cggatgcaaa tacattgctc
    17101 gcagtcatgg ataaagcatt ccgctcgtca gaaaaaatca atttgatcat ctcttccaaa
    17161 catccgcgtg ctcaatttta ctcagcagaa gaagcagccg tcttagtgaa tgaaggcttg
    17221 aaaatcattg attgggcttc gacagcaaaa gaggaggaac ctgaattggt cattgctgct
    17281 gcaggaacgg aatctaactt agaagcatta gcagctgtaa ctttgctgct ggaagaattc
    17341 ccaaaactaa aaattcggtt catcaatgtc gtagatttat tgaaattacg tcatccaagt
    17401 caagatccgc gtgggttgag cgatgaagaa tttgatcaat attttacaaa agacaaaccg
    17461 atcttatttg ctttccacgg ttatgaaaca ttggtccgca cgattttctt tgatcgtcat
    17521 aaccatcatt taatgattca tggctacaaa gaaaatggag atatcacgac accatttgac
    17581 atgcgtgtag taaatgaact tgatcgttat catttggcaa aagacgcagc tttaaaaatc
    17641 aaaggaagtc aagcagaaga ttttgcggaa aaaatggatc agaaattaca agaacatcaa
    17701 aactatattc gtgaaaatgg tattgatctg ccagaagtat tggattggaa atggaaaaat
    17761 cttgaccaat aaaatgataa aatcgatccc gcaaaacaga attttttaca ttgtattatt
    17821 tgtcaaatta ag
//
LOCUS       DAXWOO010000043        17361 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.043, whole genome shotgun sequence.
ACCESSION   DAXWOO010000043 DAXWOO010000000
VERSION     DAXWOO010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.043"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..784
                     /locus_tag="ACGE70_001186"
     CDS             <1..784
                     /locus_tag="ACGE70_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692835.1"
                     /translation="ENPDKNKFETETKDLRTVGYTSSEKVLTNKDMTNNQVNYSAVVR
                     TVKERANNNPVKEFNENINYKFTDNTKTKTGYGVSVDLEPNYRNEIGKQIDMKVYSYP
                     MNELIDSKIDEIYQSGGKETEVKMDRLKNDGKVESGINTQNLSFQFPHMNIERKTGNI
                     YADSQKNDESIKYELIDGGRKLYVPIWCDLGNYSIAIKSSNFGGNYISLDMNQNIDVY
                     AYMYATIDSETKKEDELLLEPVYPESTLPDGWNKNEIDWLNK"
     gene            844..1206
                     /locus_tag="ACGE70_001187"
     CDS             844..1206
                     /locus_tag="ACGE70_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692836.1"
                     /translation="MRVQSYGNSVFDVKREFQDSRRKTKKLIAYFILSAIVFYIVKFV
                     LELNSMVFTDMVHKMLNGEPINFNFDYMLEKAQSNQVVAFLVSTYYFIEKSIFSLKDF
                     FLGIFTKLTTFYNKILKN"
     gene            1220..1372
                     /locus_tag="ACGE70_001188"
     CDS             1220..1372
                     /locus_tag="ACGE70_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692837.1"
                     /translation="MLDQNTDRSWWMIGAVIVGAVLIGTAKVAFPEVFDTVITFFKNM
                     IPTNLG"
     gene            1440..1862
                     /locus_tag="ACGE70_001189"
     CDS             1440..1862
                     /locus_tag="ACGE70_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692838.1"
                     /translation="MIYVALFLDGSDVMLRDMPKMILLMFVGFFLILVIAYYSLNGYT
                     QDSSVKGMQETLRTTAIANRDDSIRVEKGRFALIKSDFEKDFKEKFGKSKNLNNETTD
                     YIFDYLTDGKDGIKAIKVKVESGSKEYQATCILNVAND"
     gene            1874..3289
                     /locus_tag="ACGE70_001190"
     CDS             1874..3289
                     /locus_tag="ACGE70_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HFF8692839.1"
                     /translation="MRVVEEKKISPKIEENPKSKINIASIIKKNGIKENSEAIEGNLD
                     FLTKLLEEDSGNYEPPIEDAKLNMIRDDLTRNYSQEVLQALTDDEIRRKLVQVVLKEH
                     GTFFKKDKEVAEYVVRECIGTGIIERIMTNDEITDVGWNGSQLSIETNSNKWLFDGDK
                     LNITQEYIERVISKYAVVNDRAFNSGNPIMDGMFKNVRISATHIDNSPDGSTFSMRVA
                     RPRLALNKENFEDFAPDYILELFEKMMKTKANICISGETGTGKTELQKLLLSFIDNHD
                     RMIMIEDVQETHAKELFPDKDIFSWITTPKKAISDLVKASLRNNPRWIIVSETRGSEA
                     YEMLQAVLSGHYVVTTLHAVNARAIPRRFVNMCASGFKINENAVEEDINRYFDFGVHI
                     KKTVVDGKVIRYLNEIVEFSANEPTTVFKQKFINGKFVIENGSLSEDFKEKMAEKFIT
                     FEFPEDQTNTEESLPEEEEVI"
     gene            3306..4121
                     /locus_tag="ACGE70_001191"
     CDS             3306..4121
                     /locus_tag="ACGE70_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692840.1"
                     /translation="MTKRKGLMDVSELNAYQVAYGKPLDYRDYFNYVGLPCLFMGGFS
                     FALCYYWWIALICGVIGGVYGFKVIMPKSIKRSYELKSLQERNKFINNMTQILTDDSK
                     TVTRALSIAKQRTKGELREDIQILESRLQGADKFQIAEAFREINNKYYSDVVFTQYFE
                     QLETAIYEGRNNSDTMKQIKSYHNDVKRKTEGFMKIKDGYLQDMKQMMFIVAVFIGAI
                     TFAFGFSTFYEGFARSPIGWVFGSIYFLIMVSFMKSFFKHYFDDEIMSLGVSN"
     gene            4169..4918
                     /locus_tag="ACGE70_001192"
     CDS             4169..4918
                     /locus_tag="ACGE70_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692841.1"
                     /translation="MKITKTEHKSFWIKLLGDVYYQKLREMNNSNIAIIKWQKQRLKK
                     SLIFILVGFLMYLFLGKEWYYLAGGIATSVGFYVWQAKSINGIYNQFRFERHLQFSKF
                     TRLLIPYLKQKTDGGNLYGVFAKIVKRLDYDTDKKLLMKLMQEMTDQPNDIKPFTEYA
                     EQTSGTDMSVLFMSTVYDIRQGSADLDVIEELDRLASEELMAGIDSIIDYKSRRFVFF
                     PTKIAMTSFILVVGFAVGVLFMNLKDINFGQ"
     gene            4954..5298
                     /locus_tag="ACGE70_001193"
     CDS             4954..5298
                     /locus_tag="ACGE70_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692842.1"
                     /translation="MEIIKWFIGLIMIMTMVSIGLFCVQLQDINTFKQQVNYQIERKG
                     GLTTEAVEAIDKYSATNYDGRFTIESDQLNQKVSYGEEIDYTIKATIPIQILPLPDVA
                     LSFNGNSVSQIR"
     gene            5312..5458
                     /locus_tag="ACGE70_001194"
     CDS             5312..5458
                     /locus_tag="ACGE70_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692843.1"
                     /translation="MQENNVSRIIYLIGGLIILSLAIAGLVEHWPEIKLLIRNFYDNK
                     MIKM"
     gene            5470..5691
                     /locus_tag="ACGE70_001195"
     CDS             5470..5691
                     /locus_tag="ACGE70_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692844.1"
                     /translation="MEAIQENRKDTQETIEKNKKTRKKKVDKFTEMTDKIIEKKGLKP
                     KDFDRQAIKEARQKLYDDFVKENISQLLD"
     gene            5710..5985
                     /locus_tag="ACGE70_001196"
     CDS             5710..5985
                     /locus_tag="ACGE70_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HFF8692845.1"
                     /translation="MKNLVISKFAMLPRMDFFDNVSKFMSNMTSKLQGISLSVIIFCI
                     VVTGLMFLFGEGPSRTAKKWLTYIVIGAIIIFGASTIGQTIKSVGGF"
     gene            6094..7920
                     /locus_tag="ACGE70_001197"
     CDS             6094..7920
                     /locus_tag="ACGE70_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692846.1"
                     /translation="MNQRFEYNFEDVEPKLLKKGKGLKKKNKYTLKQAEALVGWIQHN
                     WSDKTTITLICSDTEEGTKYAFDGIEITPFSWGGQEKLLFPDIEGQILQAPDSMISDP
                     NKLILDINDQYKHEPDVAEPTKESKSKLNLKLPSVVLPFAKKKNKLMNELIEPEETDI
                     YEEELEEQEEIEELAVSPEEPEEVEEIETVSENEEEVEEIFDFEDEEEVENVQKGDLS
                     TSSQNELVAVNDSQVPPYMPSQTPNYSPQYQAPNVLQPVRKHETVQLVTLNEYCDLGE
                     EIETSISQMVEKLKPESLFKFIGIPSTGLTNTRIDEYRSNHAMLRLAEVKFENLREHY
                     SRQIISFKAEALDRLRTAVDVAWKKPYDLDVKKDKAEELSKMEAEAEAKLNEFVKRQE
                     NNVNDKIKKFEIEQEIDLKNFIARQEADKAVFIATEKDRTQNLIDSKAESINADLLET
                     KDKFIDEEMYKMKIEMNKNLFDGKQKIKSELATELAKANDNVWENALKFIQEIQDEIE
                     AKTPIWAAEIKEFNSLEEQEHERMKQTKELQLKEESIEIKRLEAEMNLKRMEELDHEN
                     KMLKIKLETALSKNELLAMEQDTKQVVNGSNRVGALNLFSKR"
     gene            7933..8649
                     /locus_tag="ACGE70_001198"
     CDS             7933..8649
                     /locus_tag="ACGE70_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692847.1"
                     /translation="MSVFNNKVMLGNCYVNHIGFYDGGTYRVLFLRLSVFSYSKDGKG
                     HYENINAQISCPLDRNGKPTTTSSHPKALMNKFIFEKLDRNLVEGMKISIHGNIRGTE
                     QVLLNSGWKNFGQLSPLEKEEYQKIPKNELVTRNQTYIYIEEVSMPEKEEFRKSQSKV
                     KKEDVEVAEPNQADEILSDDTDPVNDEGEVIEVFEAEIIEEEEEVKSVDRRMYREKPK
                     GYVNFEDMYYDEYSNEPPIN"
     gene            8662..11520
                     /locus_tag="ACGE70_001199"
     CDS             8662..11520
                     /locus_tag="ACGE70_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFF8692848.1"
                     /translation="MKLVQNNEVKERKNGFVKNSNKILGKLKKVGGFKKNKYHTGKIE
                     GEDLPKSLQDKLNVPLVFIGVFLGSIVFLAITYLLNFLKALLVAIGQSESGLLGVEFD
                     PKFSLAYLKPLGDTKQYFMCAVVAVIGVAYLIYGKLNHESEKNLVYGQKGDSRLSTIK
                     EIKRDYHEIPEKTEVYDGIGGVPISHYQDKYYIDRDTVNTVVLGASRSGKGETFVVPL
                     LDNLSRAKIQSNMVVNDPKGELFSASKETLEKRGYRVLVLNIDDPLESMSFNPLQLVI
                     DSWANKDYHEASKRANTLTSMLFASGMGTDNEFFYKSAKSAVNAIILTIVEHCFNNDC
                     IEKITMYNVAQMLNELGSLFYTDPKTGKEKNALDEYFNTLPQGNQAKIQYGSTSFAGD
                     KAKGSILSTASQGIEMFTSDLFGKLTSKMSIDLKEIGFPKSIQFKVNTNLVGKRISIS
                     FLRKENDVIRLIKKYRVKVKALGLCVLNFNEYLQDGDMLDIRYEEEKSRAWYRIEFPK
                     KQANSHDTSLVTKKFKSGKNYSDLEISTLRLKYTDQPTAVFMVIPDYDPSNHVLASIF
                     ISQLYTELASNCKQTPDKKCFRRVHFLLDEFGNMPAIDNMDGIMTVCLGRNMLFDLVI
                     QSYSQLETRYDKAFKTIKENCQNHILIMSNDEETIEEISKKCGHKTTINRSASSKHLD
                     TDSSVTSSAEQERVITVERASQLIEGEQIILRNLHRQDTKRNKIRPFPIFNTKETNMP
                     YRYQFLAEDFDTQRDINEIDIACEHINLSLQDNQIPYARFIKDLKTRLEYSIINNIPI
                     SEEDYQDYRNLSGSQQTINEEELLKLVNTEVKSQDMEGQVLSEQEMKINIYLKESQKE
                     AIELVKVINGRLADENSADMVIKTINSQVLDTPFIQSLINDSIAIHNVQESKDQIMIL
                     EQQLANAYELAVLKTKNSILKNKIIKLKNAMTKLAKAI"
     gene            11538..14312
                     /locus_tag="ACGE70_001200"
     CDS             11538..14312
                     /locus_tag="ACGE70_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFF8692849.1"
                     /translation="MTESINWRKLNVQWLKYKWPIIVSLIIILFMAYPQRVFALPWIT
                     DNKEVLKFLTKYQDYLQFTKGGFGSIVVQSLSWGLIRGAYFVCKSAENLVPQSLSLFS
                     FVESSSLGAVYQSVLNTVVVGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSIALIL
                     LMPTMISSGIQFAKTFYKDSTTITKADGGVAWSLIEDGVTDLVYINKNDAYDRLGKDG
                     VQKNALKEKNFFSYDFSEVITVDTAKDLEDDNKKAEYLQSRLTPDGEGNMQAVKIDDG
                     WMSMFSDSFKGGYYRFTKNTFALTLGLLSLAVAYVFSIFIIVTAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFVSYLGTGGGSSVNPFIKAVA
                     YVCAVFVLIKGSGTVMRYFGVDIGLKEGYGQLVSAFGMGAMLFRKGSNGFNRAKGSGN
                     GNGSDSGEGEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERATK
                     PFKSMRDMANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMHGQAKS
                     AEELINQKRQEAKYTPEAMNREEMLRKRVEGRTGTNTDEKEALTKERIQEAKNSNTGL
                     EKLVKENLQNSTSSQGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNGQGTKTIDV
                     RENLQSSSTSQPKTVDVRENVQNSTSSQGFKSVDVRENVQGSSASQPKTVDVRENVQN
                     STSSQGFKSVDVRENVQGSSASQAKTVDVRENVQSSTSSQGFKTVDVRENVQKDSGEM
                     KEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKPRRRFTYEDNELFRDTLN
                     DPNPLFDNLN"
     gene            14336..14671
                     /locus_tag="ACGE70_001201"
     CDS             14336..14671
                     /locus_tag="ACGE70_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5592 family protein"
                     /protein_id="HFF8692850.1"
                     /translation="MPAFYEVKMMYRNPKNIKQEIKLFFFYLVDLGIVAGSVMASVYF
                     AKLMPISPMMKFALYLLAFVVGVWMCVRTPNHPKERNILMLYYLFKMDRNRYHEVEFK
                     RNSRGKIEL"
     gene            14668..15285
                     /locus_tag="ACGE70_001202"
     CDS             14668..15285
                     /locus_tag="ACGE70_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692851.1"
                     /translation="MIVAESNENKLSGVFDKFLELVIPKDKDMCELLPYKKILKDGTL
                     VTNDNKYQAFFKVKTTDLTSISEDGFMRMVNKLTYLNRVYLEPYKIYSLTYPTETVNQ
                     AIYWKHRIQEAKRSLLSNNDPNLETYLKWNLKLAYDNYERVDLVSTLPELMFVICVYG
                     KTQKEVKNHADVMKQAGGRELDLQRITEGDLINVITKLMNPNTSL"
     gene            15302..15769
                     /locus_tag="ACGE70_001203"
     CDS             15302..15769
                     /locus_tag="ACGE70_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692852.1"
                     /translation="MLKHCRKGFCKVIKGKNSADLIFEWKESNLINVRGKNGSGKTER
                     VMASIDNSRPRSRALVITDEKNHYKGIRTIEKKYLTNNDLKVVDDILNIYPDFNLVVF
                     DETIPVTEIKGFYQSFSNLANKYEEVLFVWITEENSNSKSAFFSEKNAFLHSI"
     gene            15785..>17361
                     /locus_tag="ACGE70_001204"
     CDS             15785..>17361
                     /locus_tag="ACGE70_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion system protein"
                     /protein_id="HFF8692853.1"
                     /translation="MIVSKKLARNLVSEGYDLRLVEKYQPSGGIKFDERYISTGDGYV
                     TSIAVIEFAEDVNSRWLTDIMAIPNTIATFDVCTADKEDLLHKINTSIGELDYRSTNE
                     NRTTDKTDALLDKYNLEDFAQEITKRGEVVKTVHARIFVYSDSLEELEKRISEIRKDL
                     ASVNHKAIVPQFRMKDEWMSLFQDGMEQESKLFAKKGLTVPAKNIGGGIPFHHKSLKD
                     PRGFYLGTTSTGGAFIFDPFYSTKQRRSFNGFILGKMGFGKSTLIKQLQEGLQAKNCF
                     IRGFDKSGEFEGMVKKAGGVYVDLGGGDMVVNPLQIYATVMKKDQFGNDVVDESKSFQ
                     NHITKCSNQIRFLEPEIDTATVREYTLMLQDFYIHYGILPKEWREARDDNERNQLNTN
                     ICNLPNDSYPIYGEFLKFIENQQFKIKNATPERVRSIELIKLIVTDMCTTYPQIFNGV
                     TTMPNFDTTKIVYYNIDSISKMSAPVFHCQLFTALTTIWNSALQNGKIQKENFKKYGS
                     DYYKYAQDFMVLIDECH"
BASE COUNT         6498 a         2219 c         3455 g         5189 t
ORIGIN      
        1 ggaaaatcca gataaaaata agtttgaaac tgaaaccaaa gatttaagaa cggtcggtta
       61 tacttcttct gaaaaagttt taacgaacaa agatatgaca aataaccaag tcaactattc
      121 agcagtagtt agaacagtga aagaaagagc gaataataat ccggttaaag agttcaatga
      181 aaatatcaat tataaattta ctgataatac taaaacaaaa acaggatatg gtgtatcagt
      241 tgatttagaa ccaaactaca gaaatgaaat tgggaagcaa atagatatga aagtctatag
      301 ttatccgatg aatgaattga ttgacagcaa aattgacgaa atctatcaaa gtgggggaaa
      361 agaaacagag gttaagatgg atcgcttaaa gaatgatggg aaagttgagt cgggtattaa
      421 cactcaaaat ctttcattcc aatttccaca tatgaatatc gaaagaaaaa ctggaaatat
      481 atatgctgac agtcaaaaaa atgatgaatc tattaagtat gaattgatcg atggcggtag
      541 aaaattatat gtgcctattt ggtgcgattt ggggaattat tctattgcta tcaaaagtag
      601 taactttggt ggtaactata tctcgttgga tatgaaccag aacatagatg tttatgctta
      661 tatgtatgca acgatagata gtgaaactaa aaaagaagat gaattattgc ttgaacctgt
      721 ttatccagaa tccacattgc cagatggttg gaacaaaaat gagattgatt ggttgaataa
      781 ataagtttga aaagttttta tctatctgat agaattaaac aatgatatag ggatggtgat
      841 agaatgaggg ttcaaagcta tggtaacagt gtttttgatg ttaaaagaga atttcaagat
      901 tcgagaagaa aaaccaaaaa gctgatagct tacttcattc tatcagctat cgttttctat
      961 attgtcaagt ttgttttgga attaaactca atggtattca cggatatggt tcacaagatg
     1021 ttaaatggag aacctattaa ttttaacttt gattatatgc ttgaaaaagc gcaaagtaat
     1081 caagttgtgg cctttcttgt gagtacttat tattttattg aaaaatctat tttttcttta
     1141 aaagactttt tcttggggat atttaccaaa ctaacaacgt tttacaataa aattttaaaa
     1201 aattaaggag gaagtttgaa tgttagatca gaatacagat agaagttggt ggatgattgg
     1261 tgccgtaatc gtaggtgccg ttttgattgg aacagctaaa gtagcttttc ctgaagtatt
     1321 tgatacagta attacatttt tcaaaaacat gatccctaca aacttaggtt aattccacta
     1381 aaaaattcac gctgttaaat gcgtagaaaa ataataaaga agaaaaaggg ctacataaag
     1441 tgatttatgt agcccttttt ttggatggaa gtgatgtaat gctaagagat atgcccaaaa
     1501 tgattcttct gatgtttgtt gggttctttt taattttagt gattgcgtac tattcattaa
     1561 atggctatac acaagattct tcagtgaaag gtatgcaaga aacattaaga actacagcaa
     1621 tagcaaacag ggatgatagt attcgtgtag aaaaagggcg ttttgcttta attaaatctg
     1681 attttgaaaa agattttaaa gaaaaatttg ggaaaagtaa aaatctaaat aatgaaacca
     1741 ctgattatat atttgattat cttacagatg gaaaggatgg tattaaagca attaaagtga
     1801 aggttgaaag tggatcaaaa gaatatcaag caacttgtat tttaaatgtt gcaaatgatt
     1861 agaggtgtga aatttgcgtg ttgtagagga aaagaaaatt tctccaaaaa tcgaggaaaa
     1921 tcctaaaagt aaaattaata tcgcaagtat tattaaaaaa aatggaatta aagaaaactc
     1981 tgaagcgata gaaggaaatc ttgatttttt aactaagctt ttagaagaag atagtggaaa
     2041 ttatgaacca ccaattgaag atgcaaaatt gaatatgatt cgtgatgatt taaccagaaa
     2101 ctattcccaa gaagttttac aagctttaac tgatgatgaa attaggcgaa aacttgtaca
     2161 agttgttttg aaagaacatg gtactttctt taaaaaagat aaagaggttg cagaatatgt
     2221 agtacgtgaa tgtattggaa cagggattat tgagcgaatt atgaccaacg atgaaattac
     2281 agatgttggt tggaatggct ctcaacttag cattgaaact aactctaaca aatggttgtt
     2341 tgacggtgat aaattaaata tcacgcaaga gtatattgaa cgtgttattt ctaaatatgc
     2401 tgttgtaaat gatcgggctt ttaatagtgg taatcctatc atggatggaa tgtttaaaaa
     2461 tgtccgtata tcagctacac acatagataa ttcaccagat ggctctacgt tttctatgcg
     2521 tgtggcaagg cctagattag cattgaataa agaaaacttt gaagattttg ctcctgatta
     2581 tattttggag ctatttgaga aaatgatgaa aacaaaggca aatatctgta tatcagggga
     2641 gacaggtaca ggtaaaacgg aacttcaaaa attactgcta agttttattg ataatcatga
     2701 tcgtatgatt atgatagagg acgttcaaga aactcatgcc aaagaattgt ttcctgataa
     2761 agatattttc tcttggatca ctactcccaa aaaagcaatt tcagatttag taaaagcttc
     2821 tcttagaaat aatccacgat ggatcattgt ttcagaaaca cgtggttctg aagcttatga
     2881 aatgcttcaa gcggttttgt caggtcacta tgttgtaacg acacttcatg cggttaatgc
     2941 acgtgcgata cctagacgtt ttgtaaatat gtgtgcaagt ggttttaaaa taaacgaaaa
     3001 tgcggttgag gaagacataa atcgttattt cgattttggt gttcatatca aaaaaacagt
     3061 ggttgatggt aaagtgatcc gttatttgaa tgaaattgtt gaattttctg ctaatgaacc
     3121 gactacagtt tttaagcaaa aatttatcaa tggaaagttt gtcattgaaa atggtagtct
     3181 ttccgaagat tttaaagaaa aaatggctga aaaatttatt acgtttgaat ttcctgaaga
     3241 tcaaactaat acagaagaat cacttcctga agaagaagag gtgatttaag ttgaaagtac
     3301 aggctatgac aaagcgtaaa ggtctcatgg atgtttcgga actaaacgcc tatcaagtag
     3361 cgtatggtaa accgttagat tatagagatt attttaacta tgttggatta ccttgtttgt
     3421 ttatgggtgg cttttctttt gctttatgtt actactggtg gattgctttg atttgtggag
     3481 taattggagg cgtatatggc tttaaagtta taatgcctaa aagtattaaa cgctcttatg
     3541 aattaaagag cttacaggaa agaaataaat ttattaacaa catgactcaa attttgaccg
     3601 atgatagtaa aacagttact agagcgttat ctatagcaaa acagcgaaca aaaggtgaat
     3661 taagagaaga tattcaaatc ttagaatcac gtttacaagg tgctgataaa tttcagattg
     3721 ctgaagcttt tcgtgaaatc aacaataaat attatagtga tgttgttttt acacaatact
     3781 ttgagcaact ggaaacagct atttacgaag gtagaaataa ttcagatact atgaagcaaa
     3841 taaaaagtta tcacaatgat gtgaaacgta aaacagaagg ttttatgaaa atcaaagatg
     3901 gctatcttca agatatgaag caaatgatgt ttattgtagc tgtttttatt ggagctataa
     3961 cttttgcttt tgggttttct actttctatg aaggctttgc tcgatcacct atcggatggg
     4021 tatttgggtc tatttacttt ttgattatgg ttagcttcat gaagtcattt ttcaaacatt
     4081 attttgatga tgaaataatg agtttggggg taagtaatta atgaaagtta aggctttgaa
     4141 aaagaaaaga aaatcaagta ttagcccctt gaaaattacg aaaacagaac ataaaagctt
     4201 ttggattaaa ttgctaggtg atgtttatta tcaaaaatta cgtgaaatga acaattccaa
     4261 tattgcgatt attaagtggc aaaaacaacg gttaaaaaag tcattgattt ttatcctggt
     4321 cggattctta atgtatctat ttttagggaa agaatggtat tacttagccg gaggaattgc
     4381 tacttcagta ggtttttatg tttggcaagc aaaatcaatc aatggtatct ataatcaatt
     4441 tcggtttgaa cgacacttgc aatttagtaa atttacacgt ctattgattc catatctgaa
     4501 acaaaaaaca gatggcggaa atctatacgg tgtttttgca aaaatcgtaa aaagactaga
     4561 ttatgatacc gataaaaagc tattaatgaa gcttatgcaa gaaatgacag atcagcctaa
     4621 cgatattaaa ccttttacgg aatatgcaga acagacttcc ggaacagata tgtccgtatt
     4681 atttatgtct acagtttatg atattcgaca aggatcggca gatttagatg tgattgaaga
     4741 gttggatcga ttagcttctg aagaattgat ggccggaatc gattcaatca ttgactataa
     4801 gtcgaggcgt tttgtcttct ttcctacaaa aattgcaatg acttcattta ttttagttgt
     4861 cggttttgca gtaggcgttc ttttcatgaa cctaaaagat attaactttg ggcaataaag
     4921 gaaaggagaa agtcaatgca agaacaatcc gttatggaaa tcattaaatg gtttataggt
     4981 ttaattatga ttatgacaat ggtttctata ggactctttt gtgttcaatt acaagatatt
     5041 aatactttta agcaacaagt aaattaccaa attgaacgaa aaggaggtct gactacagaa
     5101 gctgttgaag caatcgacaa gtattcggct actaattatg atgggcgttt tacaattgaa
     5161 agcgatcagt taaatcagaa ggtttcttat ggagaagaaa ttgattatac gatcaaagca
     5221 actattccaa ttcaaatttt accgcttcct gatgtagctt tatcgtttaa cggaaatagt
     5281 gtttctcaaa taagatgaga ggaaggataa aatgcaagag aataatgttt ccagaataat
     5341 ttatttgatt ggcggattga taattttatc cttagctatt gccggtcttg tagagcattg
     5401 gccagaaata aagttattaa taagaaactt ttatgacaat aaaatgatta aaatgtagga
     5461 ggtatagatt tggaagcaat tcaagaaaat cgtaaggata ctcaagaaac aattgaaaag
     5521 aacaaaaaaa caagaaaaaa gaaagtagat aaatttacgg aaatgaccga taaaatcatt
     5581 gaaaagaaag gacttaaacc taaagacttt gaccgacaag caatcaaaga agcacgacaa
     5641 aaactgtatg atgattttgt taaagaaaac atatcacagc ttttagacta acatttaggg
     5701 ggaaataaaa tgaagaattt agttatcagc aagtttgcaa tgttaccaag aatggatttt
     5761 ttcgataatg taagtaagtt tatgtcaaat atgacttcaa aactacaagg aatttcgcta
     5821 tctgtaatta tcttttgtat tgtggttact ggtttgatgt ttcttttcgg agaagggcca
     5881 agtcgtactg ctaaaaaatg gttaacgtat atcgtgattg gggcaataat tatctttgga
     5941 gcttcaacta tcggacaaac gatcaagtcc gttggtggtt tctaagggga agcttcttag
     6001 aactaaaaaa ttcgcaagta ttgcatgaaa gagcattgag tgattaatgc tcttttttat
     6061 tttgaaaaac tataaactaa ggagatttaa actatgaacc aacgattcga atataatttt
     6121 gaagatgtag aacctaagtt gttgaaaaaa ggaaaagggt taaaaaagaa aaataaatat
     6181 actctcaaac aagcagaagc actagtagga tggattcaac acaattggtc agacaagaca
     6241 acaattacct tgatctgttc agatacagaa gaaggaacaa agtatgcttt tgatggaatt
     6301 gaaattactc cattttcgtg gggcggacaa gaaaaactat tgtttcctga tattgaaggt
     6361 caaattttac aagcgcctga cagcatgatt tccgatccta ataaactgat tttggatatt
     6421 aacgatcaat acaaacacga accagacgta gcagaaccaa caaaagaatc aaagtcaaaa
     6481 ttaaatctga agctcccttc agtagtatta ccatttgcta agaagaaaaa taaacttatg
     6541 aatgaattga ttgaaccgga agaaacggat atttatgaag aagaattaga agaacaagaa
     6601 gagattgaag aattagctgt ttctcctgaa gaaccagaag aagtagaaga aattgaaact
     6661 gtttctgaaa atgaagaaga agtagaagaa atttttgact ttgaagatga agaagaagtg
     6721 gaaaatgtac agaaaggcga tttgtctaca agtagtcaaa atgaattagt agctgtaaat
     6781 gattcacaag ttccgccata tatgccttct caaactccta attactctcc acagtatcaa
     6841 gcgcctaatg tcttacaacc agtaagaaaa cacgaaacag tgcagttagt tacgcttaac
     6901 gagtactgtg atttgggaga agaaattgaa acttcaattt ctcaaatggt cgaaaagttg
     6961 aaaccagaat ctttattcaa attcataggg attccttcta caggtcttac aaatactcgt
     7021 attgatgaat atagaagtaa ccatgctatg ttgcgattag ctgaagtaaa atttgaaaat
     7081 ttaagagagc attattcacg acaaattatt tcatttaaag cagaagcact agatcgttta
     7141 agaactgcgg tagatgtcgc ttggaagaag ccttacgatt tagatgtgaa gaaagacaaa
     7201 gccgaagagt tatcaaaaat ggaagcagaa gcagaagcta aattaaatga gttcgtgaaa
     7261 cgtcaggaaa acaatgtaaa tgataaaatc aaaaagtttg agatcgaaca agaaatagat
     7321 ttaaaaaact ttattgcaag acaagaggca gacaaagcgg tattcattgc tacagagaaa
     7381 gatcggactc aaaatttaat tgattctaaa gcggaatcaa tcaatgcaga tttacttgaa
     7441 acaaaagata aatttattga tgaagaaatg tacaaaatga aaattgaaat gaacaaaaat
     7501 ttatttgatg gcaagcaaaa aattaaatca gaattggcta ctgaactcgc taaggcaaat
     7561 gataatgttt gggagaatgc gctgaaattt attcaagaaa ttcaagatga aattgaagct
     7621 aaaacaccaa tttgggctgc tgaaatcaag gagtttaata gtttagaaga acaagaacat
     7681 gaacgtatga agcaaactaa agaacttcaa ttaaaagagg aaagtattga aatcaagcgt
     7741 ttggaagctg aaatgaattt gaagcgtatg gaagaattag atcatgaaaa taaaatgctt
     7801 aaaattaaac ttgaaactgc cctttcaaaa aatgagttac tagctatgga acaagataca
     7861 aagcaagtgg tgaatggatc aaacagagtt ggcgcactta atttatttag taaacgttaa
     7921 aggagaacgg atatgagtgt ttttaataat aaagttatgt tagggaactg ttatgtgaac
     7981 cacattggtt tttacgatgg aggaacatac agagttttat tcttacgttt atctgttttt
     8041 agttactcaa aagatggaaa ggggcattat gaaaatatta atgcacaaat ttcttgtcct
     8101 ttagatagaa atggaaaacc tacgactaca agtagtcacc ctaaagcctt gatgaataaa
     8161 tttatctttg aaaagttgga tcgcaatttg gttgagggga tgaagataag tattcatgga
     8221 aatattcgtg gaacagaaca agtgcttctt aacagtggtt ggaaaaactt tggtcaatta
     8281 tcgcctttag aaaaagaaga atatcaaaaa attcctaaaa atgaattagt aacaagaaat
     8341 caaacatata tctatataga agaggtaagt atgccagaaa aagaagaatt tagaaagtct
     8401 caatctaagg taaaaaaaga agatgtggaa gttgcagaac cgaatcaagc agatgaaatt
     8461 ttatccgatg atactgatcc agtgaatgat gaaggagagg ttattgaagt ttttgaagct
     8521 gaaattatcg aagaggaaga agaagttaaa agtgttgata gaagaatgta tcgagagaaa
     8581 ccaaaaggtt atgtgaattt tgaagatatg tattatgacg aatattctaa tgaaccaccg
     8641 atcaattagt gggggtcaaa tatgaagcta gtacaaaaca atgaagtgaa agaaagaaaa
     8701 aacggctttg ttaaaaactc gaacaaaatt ttgggaaagc tgaaaaaagt aggtggtttc
     8761 aaaaaaaata aatatcatac tggaaaaatt gagggagaag atttaccgaa aagtttacaa
     8821 gataaattga atgttccgct tgtatttata ggtgtttttt taggttcaat agtttttcta
     8881 gcaataacat acttattgaa ctttttaaaa gctttgttag ttgcaatcgg ccagtcagaa
     8941 agtgggctac ttggtgtaga gttcgatcct aaatttagtt tggcatatct aaaaccactt
     9001 ggggatacga aacaatactt tatgtgtgct gtggttgcgg taataggagt cgcttattta
     9061 atatatggga aattaaatca tgaaagtgag aaaaatcttg tttacggtca aaaaggagat
     9121 tcaagacttt caactatcaa agaaattaag agagattacc atgagatacc agaaaaaaca
     9181 gaagtgtatg atggtattgg tggtgtgcca attagtcact atcaagataa atattatatt
     9241 gatagagaca cagtaaatac cgttgttttg ggagcttcac gatccggtaa aggggaaaca
     9301 tttgttgttc ctttattaga taatcttagc cgagcaaaaa tacaaagtaa tatggtcgtg
     9361 aatgatccga aaggagaatt gtttagcgct tcaaaggaaa cacttgaaaa acgaggttat
     9421 cgtgtattag tgctaaatat tgatgatcca cttgaatcaa tgagttttaa tcctttacaa
     9481 ctagtaattg atagttgggc taacaaagac tatcacgaag cgagtaagcg agcgaatact
     9541 ttaacttcaa tgctctttgc ttcaggaatg ggtacagata atgagttttt ctataaatca
     9601 gctaaatcag cggtcaatgc gattatttta acgattgtag aacactgttt taacaatgat
     9661 tgcattgaaa aaattacaat gtacaacgtg gctcaaatgc tgaacgaatt aggaagctta
     9721 ttttacactg atccaaagac tggaaaagaa aaaaatgctt tagatgaata tttcaatact
     9781 ttaccacaag ggaatcaggc caagattcag tatggttcta cttcatttgc cggtgataaa
     9841 gccaaaggtt cgattttatc tactgcttca caaggtattg aaatgtttac aagtgatttg
     9901 ttcgggaagt taacaagtaa aatgtctatt gatttgaaag agataggctt tcctaagtcc
     9961 atacaattta aagtcaatac gaatttagtt ggaaagagga ttagtatttc atttttgaga
    10021 aaagaaaatg atgtaattag attaattaag aagtatcgag tgaaagtaaa agctttaggg
    10081 ctatgtgttt taaattttaa tgaatatctt caggatggag atatgttgga tatacgatat
    10141 gaagaagaga aatcgagagc atggtatcgt attgaatttc ccaaaaaaca agcaaatagt
    10201 catgatactt ctttagtcac taaaaaattc aagtcaggta aaaattattc tgacttagaa
    10261 atcagcacct tacgattaaa atacacggat cagccaactg ctgtgttcat ggttattcca
    10321 gattatgatc cgtcaaatca cgtacttgca agtattttta tcagtcaact ttatacagaa
    10381 ttagcaagta attgtaagca aacaccggat aaaaaatgtt ttaggcgagt tcatttcttg
    10441 ttagatgaat ttgggaatat gccggctata gacaatatgg atgggattat gacggtatgt
    10501 ctaggaagaa atatgctttt tgacttagtg attcaatcct atagtcagct agaaacaaga
    10561 tatgataaag cttttaaaac aattaaagaa aattgtcaga atcatatttt aatcatgtcg
    10621 aacgatgagg aaacgataga agagatttca aaaaaatgtg gtcataagac aacaataaat
    10681 agaagtgcta gttctaaaca tttagatact gatagctcgg tcactagttc ggcagagcaa
    10741 gagcgagtaa ttacagttga gcgtgctagt caattgattg aaggggaaca aattatttta
    10801 agaaaccttc atagacaaga tacaaaaaga aataagatta gaccatttcc tatttttaat
    10861 actaaagaaa caaatatgcc gtatagatac caatttttag cagaagactt tgatactcaa
    10921 agagatatta atgagattga tattgcatgt gaacatatca atctatcttt acaagataac
    10981 caaattccat atgcaaggtt tatcaaagat ttaaaaacac gtttggagta ttcaattatt
    11041 aataatattc ctattagtga agaagactat caggattatc gaaatttatc aggatcacaa
    11101 caaacaatta atgaagaaga gctgttaaaa ctggtaaata cggaagtaaa aagtcaagat
    11161 atggaaggtc aagttttatc agaacaagaa atgaaaatta atatttatct aaaggaaagt
    11221 caaaaggaag caatagaatt agtaaaagta attaatggaa ggttagctga tgaaaattca
    11281 gcagatatgg ttataaaaac cattaattca caagtccttg atacgccttt cattcaatct
    11341 ttaatcaatg attctatagc tattcataat gttcaagaga gtaaggatca aattatgatc
    11401 ctagaacaac aattagctaa tgcttatgag ttagcagtgt taaaaacgaa aaattcaata
    11461 ctgaaaaata aaattatcaa gttgaaaaat gctatgacta agctagcaaa agcaatttag
    11521 aaagaaggga agaaatattg actgaatcta ttaattggag aaaactaaat gtgcaatggt
    11581 taaagtataa atggccaatc atagtttccc taataatcat tctatttatg gcctatccgc
    11641 aacgagtttt tgcgttgccg tggattacag acaataaaga ggtattaaaa tttttaacaa
    11701 aatatcaaga ttatcttcag tttactaaag gtggttttgg aagcattgtc gttcaaagtt
    11761 tatcgtgggg gctgattaga ggtgcatatt ttgtttgtaa atcagcagaa aaccttgttc
    11821 cacaatcgct aagtttgttt agctttgttg aaagcagtag tttaggtgct gtttatcaat
    11881 ctgtattaaa cacggtggtt gtaggattaa tgatcctttc attgatgttt gttggttaca
    11941 aaatgattac tggaaaagga acagtggatt taaaatcggt tggaatgaac gtagtgatgt
    12001 ctatagcatt aatactttta atgccaacca tgatttctag tggtattcaa tttgctaaaa
    12061 cattttataa ggactctact acgattacaa aagctgatgg gggcgttgct tggtcattga
    12121 ttgaggacgg tgtaactgat ttagtatata tcaataaaaa cgatgcttat gatcggttgg
    12181 gtaaagatgg cgtacaaaaa aatgcgttaa aagaaaagaa tttcttttcc tacgatttca
    12241 gtgaagtaat cacagttgat acagctaaag atttagaaga tgataataaa aaagctgaat
    12301 atcttcaatc taggttgaca ccagatggcg aaggaaatat gcaagccgtt aagattgatg
    12361 atggttggat gtcgatgttc tctgatagtt tcaaaggagg gtactacagg tttactaaaa
    12421 atacttttgc attgacttta ggattacttt cgttagctgt agcctatgtt tttagtattt
    12481 ttatcatagt caccgctatt ttagaactgg catttaagcg tgtgttaggg gttcttgttt
    12541 ttgcaacaga tattgagaca ggacagcgtt caaaagttgt tctatcagat attctacagt
    12601 gctatttgac tgtaggcttt caaggatttg gattatccat gtttgcaatg tttgtttcct
    12661 atttaggaac aggcggaggg tcttccgtta atccgtttat taaggctgta gcttatgtat
    12721 gtgccgtttt tgttttaatc aaaggcagtg gaacagtcat gagatacttt ggtgttgata
    12781 ttggactcaa agaaggctat gggcaacttg tttctgcttt tggaatgggt gcaatgctgt
    12841 ttagaaaagg atcaaatgga tttaatcgtg ccaaaggaag cggaaacgga aatggatcag
    12901 acagcggaga aggagaagat cgaaaaccag aaaagaattt cggtgaaact ctatcaaaaa
    12961 aagctggcaa aactggaaga gctttgggat atgcccatga acgtggatta tcaggtttag
    13021 cttcagatgg agctacaatg gctagtgaaa gagcaacaaa accatttaag agtatgcgtg
    13081 atatggctaa tgatactaag aataagttca aagaaggttt agatgatggt acagtgtccg
    13141 ctattaataa aaatagcaaa cctatgttag ctaaaaataa agaagatgaa actggaaaat
    13201 atgctgattc catgccaaca cgtctttcgg atagaaaaga tggtgctaag gttgaaaatg
    13261 ccgatagaat tatgtctagt tctgaacgta tgcgtgaagc aatgaaaaat aatgctgaat
    13321 cgaataataa tcctgtaagt gcaattcagc aaaaagttca acaagatatt gaagaacgta
    13381 aaaatgctat gcacggtcaa gctaagtctg ctgaagaatt aattaatcag aagcgacaag
    13441 aagctaaata cacgcctgaa gcaatgaatc gtgaggaaat gttaaggaaa cgtgtagaag
    13501 ggcgtacagg aactaatacg gacgaaaaag aagcgttgac caaagaaaga atccaagaag
    13561 ctaaaaactc aaataccgga ttagaaaaat tggtcaaaga aaatctacaa aattctacaa
    13621 gtagtcaagg tggtcgatca gtagatgtcc gagaaaatat tcaaggttca ttgaatggtc
    13681 atgtcggacg gaatgttgat gtaagagaga atttacaaag ttcttcaaat ggtcaaggta
    13741 ctaagacaat tgatgttcga gaaaatctgc aaagtagcag tacaagtcaa ccaaaaacag
    13801 tagatgttcg agaaaatgta caaaattcta caagtagtca agggtttaaa tcagtggatg
    13861 ttcgagagaa tgtgcaaggt agcagtgcaa gtcaaccaaa aacagtagat gtccgagaaa
    13921 atgtacaaaa ttctacaagt agtcaagggt ttaaatcagt ggatgttcga gagaatgtgc
    13981 aaggtagcag tgcaagtcaa gcaaaaacag tagatgtccg agaaaatgta caaagttcta
    14041 caagtagtca aggattcaaa acagtagatg ttcgggagaa cgttcaaaaa gatagtggag
    14101 aaatgaaaga aaaaacacaa aaaatcaata tcgttgaaga cagaaagtca gcgaaaaaat
    14161 ttgattcaaa tcatgaaaca gtgattattg atagtgaaat ccgtgaaaat gataagggat
    14221 caaaaccaag acgaagattc acttatgaag acaacgaatt attcagagat acacttaatg
    14281 atcctaatcc acttttcgat aatttaaatt aaattaaaag ccggcatgag tgattatgcc
    14341 ggctttttat gaggtgaaaa tgatgtatag aaatcccaaa aatataaaac aagagataaa
    14401 gctatttttc ttctatttag tcgatttagg aatagtagcc ggaagcgtga tggcaagtgt
    14461 ttactttgca aaattaatgc caatttcacc aatgatgaaa tttgcactgt acttattagc
    14521 atttgtagtg ggtgtgtgga tgtgcgttag aacgcctaat caccctaaag agcgaaatat
    14581 tttaatgctg tattatctct ttaaaatgga tagaaacaga taccacgaag tagagtttaa
    14641 aagaaatagt agggggaaaa ttgaattatg attgttgctg aaagtaatga aaataaatta
    14701 agtggtgttt ttgacaaatt cttagaacta gttattccaa aagataaaga tatgtgtgaa
    14761 cttttaccat ataaaaaaat attgaaagac ggaacgttag tgactaatga caataagtat
    14821 caagcctttt ttaaagttaa aacaactgac ttaacttcta tttcagaaga tggatttatg
    14881 cgtatggtaa acaaactcac ttatttaaat cgagtatatt tagaaccata taaaatttat
    14941 tcattaactt atcctactga aacagtaaat caagcgattt attggaagca tagaatacag
    15001 gaggcaaaga gaagtttatt gagtaataat gatcccaatt tagaaacgta tctgaagtgg
    15061 aatctaaagc tagcttacga taattatgaa agagttgatc tagtatcgac tttaccagag
    15121 ttgatgttcg ttatttgtgt ttatggaaaa acacaaaaag aagtgaaaaa tcatgccgat
    15181 gtaatgaaac aggccggtgg tagagaatta gatttacaaa gaattactga aggggattta
    15241 atcaatgtga taactaaatt gatgaatccc aatacttctt tataaggcgg tgcttattat
    15301 tatgttaaag cactgtagaa aaggattttg taaagtaatt aaaggtaaaa attctgctga
    15361 tttaatcttt gaatggaaag aatctaattt aattaatgta cgtgggaaaa atggaagcgg
    15421 aaagacagaa agagtgatgg ctagtattga taattcaagg cctagatcaa gagcattagt
    15481 aataactgat gaaaaaaatc actataaagg catacgaacg atagaaaaaa aatatttaac
    15541 taacaatgat ttaaaagtag tagatgatat tttaaatatt tatcctgatt ttaatttagt
    15601 tgtttttgat gaaacaattc ctgtgacaga aataaaagga ttctatcaaa gcttttccaa
    15661 tctagcaaat aaatatgagg aagttctgtt tgtttggatt acagaagaaa atagtaatag
    15721 caagtcagcg ttctttagtg aaaagaacgc ttttttacat tctatttaaa aaggagaatt
    15781 gaacatgatt gtaagtaaaa aattagcaag aaatctagta agtgaagggt atgacttaag
    15841 attagtagaa aagtatcaac cgagcggtgg aattaaattt gatgagcgtt atatttctac
    15901 tggtgatgga tatgtaactt cgattgctgt tattgagttt gcagaagatg taaatagtcg
    15961 atggttaacg gatataatgg caattcccaa tactattgca actttcgatg tatgtacggc
    16021 ggataaagaa gacttattac acaaaatcaa tacgtctatt ggagaattag attaccgatc
    16081 aacaaatgag aatagaacaa ctgacaaaac agacgcttta ctagataaat acaatttaga
    16141 agattttgca caagaaatta cgaaacgtgg tgaagttgtc aaaactgttc atgctcgtat
    16201 ttttgtttat agcgattctt tagaagaatt agagaaaaga attagtgaga ttcgtaaaga
    16261 tttggctagt gtcaatcaca aggcaattgt tccgcaattt agaatgaagg atgaatggat
    16321 gtctttattt caagatggca tggaacaaga atcaaaatta tttgccaaga aaggcttaac
    16381 tgttccggca aaaaatattg gtggcggtat tccatttcac cacaagagtt tgaaagatcc
    16441 acgtgggttt tatttaggca ctacgtcaac tggtggtgct tttatatttg atccatttta
    16501 ttctacgaag caaagacggt cgtttaacgg ctttatttta ggaaagatgg gttttggtaa
    16561 gtcaacttta attaaacagc tacaggaagg cctacaagcg aagaattgtt ttattcgagg
    16621 atttgacaag tcgggtgaat ttgaaggcat ggtgaagaaa gcaggcggtg tatacgtcga
    16681 tttaggcggt ggtgatatgg tagtaaatcc attgcaaatt tatgcaacag tcatgaaaaa
    16741 agatcaattt ggaaatgatg ttgtagatga atcaaagagt tttcaaaatc atatcactaa
    16801 gtgcagtaat caaattcgct ttttagaacc tgaaattgat acggcaactg tacgtgaata
    16861 tactttgatg ctacaagact tttatattca ttatggaatt ttgccaaaag aatggagaga
    16921 agcgagagac gacaacgaac gaaatcaatt aaatacaaat atttgtaact tgcctaatga
    16981 ttcatatcca atctatggag agtttcttaa atttattgaa aatcaacaat tcaaaattaa
    17041 aaatgctact cctgaacgtg tccgttctat tgagctaatt aagcttatcg ttacggatat
    17101 gtgtacaact tatccgcaaa ttttcaacgg agtaacaaca atgccaaact ttgatacaac
    17161 aaaaattgtt tactataaca ttgattctat tagtaaaatg agtgccccag tattccattg
    17221 ccaattattt acagcgttaa cgactatttg gaacagtgcc ttgcaaaacg gaaagattca
    17281 aaaagaaaac tttaaaaaat atggcagtga ttattataag tatgcacaag atttcatggt
    17341 tttgatcgat gagtgtcaca a
//
LOCUS       DAXWOO010000044        17322 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.044, whole genome shotgun sequence.
ACCESSION   DAXWOO010000044 DAXWOO010000000
VERSION     DAXWOO010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.044"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(<1..444)
                     /locus_tag="ACGE70_001205"
     CDS             complement(<1..444)
                     /locus_tag="ACGE70_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692854.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIF"
     gene            710..2410
                     /locus_tag="ACGE70_001206"
     CDS             710..2410
                     /locus_tag="ACGE70_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFF8692855.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            2380..2598
                     /locus_tag="ACGE70_001207"
     CDS             2380..2598
                     /locus_tag="ACGE70_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFF8692856.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            2618..3508
                     /locus_tag="ACGE70_001208"
     CDS             2618..3508
                     /locus_tag="ACGE70_001208"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFF8692857.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            3526..3810
                     /locus_tag="ACGE70_001209"
     CDS             3526..3810
                     /locus_tag="ACGE70_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692858.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            4297..4911
                     /gene="gmk"
                     /locus_tag="ACGE70_001210"
     CDS             4297..4911
                     /gene="gmk"
                     /locus_tag="ACGE70_001210"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFF8692859.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            4916..5221
                     /gene="rpoZ"
                     /locus_tag="ACGE70_001211"
     CDS             4916..5221
                     /gene="rpoZ"
                     /locus_tag="ACGE70_001211"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFF8692860.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            5474..7882
                     /gene="priA"
                     /locus_tag="ACGE70_001212"
     CDS             5474..7882
                     /gene="priA"
                     /locus_tag="ACGE70_001212"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFF8692861.1"
                     /translation="MILTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            7897..8388
                     /gene="def"
                     /locus_tag="ACGE70_001213"
     CDS             7897..8388
                     /gene="def"
                     /locus_tag="ACGE70_001213"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFF8692862.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            8381..9319
                     /gene="fmt"
                     /locus_tag="ACGE70_001214"
     CDS             8381..9319
                     /gene="fmt"
                     /locus_tag="ACGE70_001214"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFF8692863.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            9319..10677
                     /gene="rsmB"
                     /locus_tag="ACGE70_001215"
     CDS             9319..10677
                     /gene="rsmB"
                     /locus_tag="ACGE70_001215"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFF8692864.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            10690..11430
                     /locus_tag="ACGE70_001216"
     CDS             10690..11430
                     /locus_tag="ACGE70_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFF8692865.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            11427..13496
                     /gene="pknB"
                     /locus_tag="ACGE70_001217"
     CDS             11427..13496
                     /gene="pknB"
                     /locus_tag="ACGE70_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFF8692866.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            13661..14560
                     /gene="rsgA"
                     /locus_tag="ACGE70_001218"
     CDS             13661..14560
                     /gene="rsgA"
                     /locus_tag="ACGE70_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFF8692867.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            14573..15223
                     /gene="rpe"
                     /locus_tag="ACGE70_001219"
     CDS             14573..15223
                     /gene="rpe"
                     /locus_tag="ACGE70_001219"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFF8692868.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            15224..15862
                     /locus_tag="ACGE70_001220"
     CDS             15224..15862
                     /locus_tag="ACGE70_001220"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFF8692869.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            16017..16871
                     /gene="mreC"
                     /locus_tag="ACGE70_001221"
     CDS             16017..16871
                     /gene="mreC"
                     /locus_tag="ACGE70_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFF8692870.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            16875..>17322
                     /gene="mreD"
                     /locus_tag="ACGE70_001222"
     CDS             16875..>17322
                     /gene="mreD"
                     /locus_tag="ACGE70_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFF8692871.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLL"
BASE COUNT         5810 a         3128 c         3758 g         4626 t
ORIGIN      
        1 aaaaatccta taaaattcat ctaaatagcg ataataaatc cgattcagct gatcattaag
       61 atcgaagtat acaaaattat tctgtagttt ataaaaaaac ggcgaatgaa gatgtgtttt
      121 catatgacca aaatataata attctgaaat ttctaaaggt gtcaattctt tcagcatcgt
      181 taaatcagta aagtcgatcc acttcaattc atcgaatcca cgcctgttac gagaagagga
      241 aaagaaacgc tgaatatttt ctgtaccacg gattactttc agccctgtat gggattcata
      301 ttctccagca gaagcagatg gatcaagcaa gagcaagttt tgaggaaaat gcacgatact
      361 gtttgcaaag tcagtagcag tcagcccttt tgttaaaaca gcattacttg tggtatcaat
      421 atgtacataa actgactcag tcatcatgtt cctcctctta tttcctctta atctctttta
      481 ttttacaaca tttgcattaa aaaagcttgt ttcttttatt ttaaaattcc attacaaaat
      541 cacgttttca ttgaaaacac tctcattttc aattaatttt tgtgattttt gtatttttta
      601 tttaaaggat gttttatgaa ttgttttctt atggaatcta caaagtcgaa tacgagactc
      661 atgacctttt tatgacgtac tggtatactt gaagaaaaag gagagcgcga tggagataaa
      721 aaaagtttac ttttcacaga aaatgtcgct gtattcgcct atccagcaag caaaacaacc
      781 ccttccattt tttcaaagtc atcaagaaag tagagacgct ttttggcgtt ctgcaaaaaa
      841 gcaatgtgct tttaaagcag tatcgaaaaa atataccgtc tattttacta ttgctaaaag
      901 tagaggagat gttattttta ataatctgtt gattgaaggt gaaacttttg aatggaaaaa
      961 attttttcgg tatatggaag cttgcgctcg tttttttatt aaagaaaact gttgtttttt
     1021 atttcaatct cctctatcag aagaatggtt gcgtattttt cataaaaacg gctatcaagg
     1081 aaccacacaa ttaaataaaa agctggtcta tcatacagca cttgttttag gtggaggagg
     1141 agcacacggt gcttatcaga ttggtgtgtg gcaggcgctg aaggaacatg atatcaactt
     1201 tgagattatc acaggtacct cggtaggtgc gttgaacggc gcattgattt tacaaggaga
     1261 tatgaaaaaa gctgtgaatt tatggaaaaa gctgtcgaca agacaagtgc ttgcgttacc
     1321 agaaatggct gcagttgaag accttcgtga acgcttctat cgagaaagac gccaaatgac
     1381 gaaaacagca ctaattgaag gcggggtttc ttctgctcca ttggaaaaga tggtcaaaga
     1441 caatttggat ttgtccttac tcaaacataa tcccaaaata cgtttatata cggtgtctac
     1501 taaactgcca gaactagcgg aagtagtgac tgatatccaa cagacaaaaa cagaagaaat
     1561 accagattgg atactcgcct ctgcttcatt ttatcccgct atggcttatc gaaagatcgg
     1621 aaaaaataaa tatgcagacg gtgggtatcg caacaaaata cctattgata ttgcgattaa
     1681 caaaggagca acagaggcat ttgtcgtaga tgtgcaaggg ccaggcccag cgaaaaaaat
     1741 tcgtatgcca gacattttta tccattggaa atgtcaaaca ttgtggacac taggcagctt
     1801 tctgcttttt gatagccaaa ggaaccaatt aaatttacaa ttaggctatc tggaaatgaa
     1861 aaaacgatta ggctattact atgggaactg gtatacattt cattctgtta aacctgcaag
     1921 aacatactgg cgaggatttt tgtcttactt aacaaaagaa attcagttag aattatcttt
     1981 tttcaaaagc ataaaatttt ggcaaaaact gcgtaattta tataagaaca gggtcgtacc
     2041 agagacatgc ggattggcga tgctagaact tttagcaaaa aaacattttt tactgcccaa
     2101 tgaaatctat caagtagaca tgatgattca aatgatctgc caacaagata taaaaagtat
     2161 gccggatgat ttattggcac aaattggtca attaagtact gaagaatggc gaaggtatcg
     2221 aaaataccag caaaaaatac aaaatgagcg cacgaaaact gtttattttg attatctgct
     2281 acaaggaaaa cgcaaggatc gtttgcagca tgaattgctt aagcaaccag ttgacacatt
     2341 actgatccta tatttatatt atctcaagga ggaacaacca tggcacaaga attttcatat
     2401 gaaatcgtag aagaaatcgc tatattgtca gaaaacaaca aggggtggcg taaagaactg
     2461 aacctagtca gctggaatgg tcgcccgcca aaattcgatc ttcgagattg ggcgccagac
     2521 catgaaaaaa tggggaaagg cctgacattg acgaacgaag agtttgaaca actgcaaaaa
     2581 gcaatagaaa atatgtaatg aggaaggacc ctgtcgaatg aaaagtatga ctggttttgg
     2641 caaagccatc cgcgaaacaa aagcgtatca gttagatatt gaaataaaaa gtgtcaacca
     2701 gcgttttcta gatgttcaaa tacgaagtcc taaaatcctg aactttgttg aaaacgattt
     2761 tcgtcagctt attaaacagc agttaagccg cggtcgaatc gaagtatttg tgaatctagc
     2821 ttatattggc aaaaacgaaa aacagatcgt catcaactgg gaattgattg attcacttat
     2881 cactcaattg acagacggaa ttcaacaacg atacggtagt caaaatcaag tgtcaattag
     2941 tcggatggcg gaacgcttcg cgctggacga aaacttcgta acggtagaag aaaaaccgga
     3001 agagagcatg gaagaattgg cacagttagc tctagataca ttgaaaagcg cacttgattc
     3061 tattgaggcg agtcgagaaa aagaaggtca agctttagct ttagtgttga aaaagaatac
     3121 tgaagaattc aaagaagtac taaaaaaact cgatagtttt gtagaaatct atgaaaagga
     3181 atttcaaata aggtatcaaa aaaagctgga ggaatttctt ggagcgaaag tcgatgagca
     3241 acggttgttg acggagctag ccatcttgtt ggaacgcgga gatattcacg aggaactgga
     3301 tcgtctggcg atccatgtac aaaaactaga tgaacttatc gaagcacaat gtcctgtagg
     3361 tcgggaatta gatttcctga tccaagaaat gaatcgggaa atcaacacga tcggttccaa
     3421 atcaaatgca atcgaaatta aaaatcaagt cgtccagtct aagacgatct tagaaaaaat
     3481 cagagaacaa gtacaaaata ttgaatgacg aaagaggtag agataatgaa atattatgac
     3541 gtaacgttcc acgaattaag tggaaaaact gtggttaaac gggatatccc ttctgaaaaa
     3601 aacggctttg acgtatggaa agatgcctgt gcagactata atgagaacga actgtttatt
     3661 ttgatcaacg acggtgctta cgtgacgatg aatcggaaat ttattgttcg aattgacaca
     3721 gaagaggtag aagatccgac agaaaaagca cgttctcgaa aagatgagat catgggtgta
     3781 gtgaatactt tgtccaatat gggcttttag caaggatgct gaacaaagaa atacaataaa
     3841 taatcagaat tggatttctt cgcttagctt ctttgctcta ggaatacatt taaaggttgt
     3901 gacaaaattc gtgtcacaat ctttttttat tttgaataag tgatgttcaa aaaccatttt
     3961 ctcggtatta agccaaattt atgcaaaaat gtgagaaact attttcacaa aatttttctt
     4021 catactcgaa actaatcggc ttcttcacga cctcttatac acggcgttca aaagcttgca
     4081 tctgcgataa taaccccaac caaacgaaaa acatgagaag ctgtttatgt ttggctgttc
     4141 ttattacttg atgcaggaca gcttttttac gacctcttgg tacagggtag cttttttacc
     4201 aatttctagc tataattaaa gataaccttg aaagttgcag aaaaaaagtg caaaataaac
     4261 gataagtaca aaaagactag ttgaaaggac gtcgtcatgt cagagcgtgg attattaatc
     4321 gtgttgtctg gtccatccgg tgtaggaaaa ggcacagtac gaaaagcaat ttttgagagt
     4381 gaggataatg attttcagta ttcgatctca atgactaccc gtaaaatgcg tgaaggggag
     4441 gtcgaaggcg ttgattattt cttccgaagc aaagaagagt ttgaatcaat gatcgaagct
     4501 ggagagatgc tggaatacgc agaatatgtc gggaattatt atggaactcc tttgtcatat
     4561 gtcaacaaga cattggatga aggaaaagat gttttcttgg agattgaagt ccaaggagca
     4621 aaacaagtca aagaaaaagt gcccgatggt gtattcatct ttttgactcc gccagattta
     4681 gcggaattaa aatcacggat cgtaggccga ggaacagatg cagatgaagt aatcgaagaa
     4741 cgaatgaaag ttgcaaaaga agaaatcgaa atgatggctc tttacgacta tgctgttgta
     4801 aatgatgaag tgccgctagc agttgaacga atcaaaaaca ttattgccag tgaacatttt
     4861 cgagtagatc gcgtaatcgg taaatatatt aaaatgttaa aggagatgta gtcttatgat
     4921 gttaaaacct tcaattgact cactattaga tcgtgtaaat tcaaaatatt ctctagtgat
     4981 tttagcaagt aaacgcgcgc atgaattgga tgcaagtgca cagcctactc tagattcttt
     5041 tgattcagta aaaagcgtcg gtcaagcttt agaagaaatc gatgcaggaa atgtagtcaa
     5101 tgatccacat cctgaattaa aacgtgaacg tttgaagatg gaagaagagg aacgtcaagc
     5161 acaaaaagag cgtgaacaac acgaattgga atcacgtatc cgtgaagagc aaaaaatgta
     5221 ataaagaaaa atagagcgaa catctaaaaa gaaaagcaaa cccagctgtt tccttttcaa
     5281 acattcgcca cttattgtct ggtagaaaag gctgtgtctg aactttttga cacagttctt
     5341 ttttttctta tctgctgtta attttttgtt aggatttggc agaagataag acaaatccat
     5401 cgatctctag taaaataggc aaggaacttt ttgtcgcttg ataagaaaag acaaaaaaag
     5461 gaggaaacga accatgatat taacagctga agtcattgtg gacgtaccta ctatgcagac
     5521 cgatcagcca ttcacttatt tggttccctc tgaaatggaa acagctttgc aagtcgggat
     5581 gcgagtagaa gtcccttttg gtaatggcaa tcgtcatgtc caaggatttg ttatggctat
     5641 ccaaaaatca gaagaatcag ctaatccatc tttgaaggca gttattcgtt tgctagacct
     5701 tgctccggtg gtcaatgaag aattactttc attagctgat tatatgaaaa aaattactta
     5761 cgcattcaaa atcacttgtc tacaaacgat gcttccgagt gtgatgaaag cagaatatga
     5821 taaattgatc tatcctttgg cagacacacc agaagttcag gtattgtttg atcagcgaga
     5881 agtgatttcc tggaaggaag cagaagaaga aggcagcctt tctcaattga ttcgctggcg
     5941 ccaagagcaa ttagtagata tcaaatatga agtccataca cgaaataaag taaaaacgat
     6001 tcgtttggtc cgttctttgt taactgaaaa gcaaatcgaa gaagaatggg caaaattacg
     6061 gcaaaatgcc aagaaacaaa aagaacttct tttgtgttta tctgaactga gccaagaaga
     6121 accaattgct tatttcaaaa ataaaggaat cagtactgct gtcttgaatc aaggaaaaga
     6181 aaagggctgg ctggagttta tagaatcaga acgttatcgc gacccttata aagaccgagt
     6241 atttgaccaa acgacggcat tggaactgaa tgcagaacaa aaaaatgctg tcgaacaaat
     6301 catcacagca ggtcagcagc aaaaagatca ggtctttttg ctggagggaa tcactggcag
     6361 cgggaaaaca gaagtatatc ttcaggcaat cgctgatgtt ttgtcagaaa ataaaacggc
     6421 tattatgtta gtgcctgaaa tcgcattgac tccgcaaatg gtcgagcgat tcaaaggacg
     6481 cttcggagaa tcagttgcgg tcttacatag cggactttcc caaggtgaaa aatatgatga
     6541 atggcgcaag attgagcggg aagaagcgca agtagtagta ggcgcacgat ctgctatttt
     6601 tgccccgctg aaaaatatcg ggctgatcat tatcgatgaa gaacatgaat caagttacaa
     6661 acaagatgag acaccgcgtt atcatgcgag agatctagca atttggcgca gtaaatatca
     6721 tcattgccca atagttctag gaagcgcgac gccctcttta gaatcaagag cacgagctca
     6781 aaaaggcgtt tatcagttat tgcagttaaa ccaccgagcc aaagcagctg cacagcttcc
     6841 agctattgag atcgtagata tgcgtgaaga gtttcagaat catcggacct ctactttttc
     6901 tgctaatttg caggaaaaaa tacaaaatcg attagacaaa aaagaacaaa ctgtcctact
     6961 gctcaatcgt cgaggatatt cgtcttttgt gatgtgtagg gactgtggat ttgttcttcc
     7021 atgtcctaat tgcgatatct ctcttacact gcatatggat acacgttcaa tgcgctgcca
     7081 ttattgcgga catgaagaac ctattcccaa tcgctgtcct aattgtggcg gcaataaaat
     7141 ccgctactat ggaacaggta ctcaaaaagt agaagaggaa ctccgagaac tttatccgca
     7201 agctaggatt ttgcgtatgg atgtagatac cactcgaaga aaaggggcac acgaacagat
     7261 tttacaaaaa ttcggtgcaa aagaagcaga tatattatta gggacacaaa tgattgctaa
     7321 aggtttggac tttccagaag tgacattagt tggggtgttg aatgctgata cctccctgaa
     7381 tttaccggat tttcgttcta gtgagcatac attccaattg cttacccaag tttcaggacg
     7441 agcagggcga gcggaaaaag caggcgaagt cgtgatccaa acattcaatc ctcaacatta
     7501 cgccatagaa ttggctaaaa aacaaaatta tgagcagttt tatcagcagg aaatgcatgt
     7561 acggcatcgt ggaggctatc ctccttatta ctttactgtg aaaataaccg ccagccatcc
     7621 tgaagaacaa gtagcggcaa agaaaatctt tcagattgcc aatcagctaa aagaagtgtt
     7681 aagccctcaa agcttgctat tagggccaac gcccagcatg attttgagag tgaaaaaccg
     7741 atattattat caactaatca tcaaatataa acatgaaccg aatcttcctc atgttttgga
     7801 tgaaattttg aatggaagcc agaaagaaca gcgacaaggt ctttttgtgg caattgacaa
     7861 cgaaccatta aactttattt aagaaagggc caaattatgc gctatcctat attgatccac
     7921 cctaatgata aattaaaaag aactgctcag ccaatcgatg taatcacgga cgagacaatc
     7981 gctttattag acaatttata tgagacaatg attgcgaatg acggaatagg gatcgctgct
     8041 cctcaagtag gacaaaacaa gagaattgct gtgatcgaag tagatgaagg cgagaaattt
     8101 gaactgatta acccagaaat catcgaagca aaaggtgaaa gccttgacgt agaaggttgt
     8161 ctcagtattc cgcatgtcta tggaacggtc aagcgagcag atgaagtgac cgtgcgttat
     8221 tatgatcgag acggagaaga gatagaagtg acggcttttg gctatttagc ccgtgccttc
     8281 cagcatgaaa tcgatcactt agacggtatc ttgttcattg agaaaatgat acagcagatc
     8341 ccggaagaag aacttgaaga atatatggag gaacatcttg atgactaagc tagtatttat
     8401 gggcacacct gctttttctg tgccgattct tgaagggctg atagaatcag gttacgagat
     8461 ccaggcagtg gtaacacagc ccgaccgtcc agttgggcgg aaacgagtaa ttacgccgac
     8521 accggtaaaa gaagcggctc tgaagcacgg tatccgtgtg ctgcagccag agaaaatctc
     8581 cggttcgcca gaaatggaag aaatcattga attggtgcct gatgtgatcg tcactgcggc
     8641 atttggccaa ttcttgcctg aaaagttatt gcaagtacca aaattagggg caatcaatgt
     8701 ccatgcttct ttacttccta aatatcgggg aggtgcgcct gttcattatg ccatcatgaa
     8761 tggggagaaa gaaactggtg taacgatcat ggaaatgatc aaaaaaatgg atgcaggcgg
     8821 aatctatgcg caagaaagca taccaatcac taaacaagat gatgtaggga cgatgtttga
     8881 aaagcttagc cttttaggaa gaaagctatt gctagaaaca ttgcccaata ttttggatgg
     8941 acaaaaacca gtacctcaag acgagtcaga agcaactttt tcgccaaaca tcacacggga
     9001 acaagaagca atcgattggc ataaaactgc agaagaaatt gacaaccaag tacgcggaat
     9061 gcgtccttgg ccaattgcat tcacgaccta tgagggcata cgctggaaat tattatcggt
     9121 tgaagtattg gcagaaacta cagacaaagc acctggtacg atcatccgga aagacaaaaa
     9181 aaatctatgg attgcttgcg gtaaacagac cgtcttagcc atcaatgaat tacagccagc
     9241 tggtaaagga aaacaggccg tacatgattt ccttaacgga agcggtcagc aagttcagat
     9301 tggtcagcag gtgaaataat gaaaaaaatc cctaaaaaaa tgaaaaaatc agtacgatat
     9361 atggcattac tgacattaga gcgagtgaac aagggtggcg cctactcgaa tcttttgttg
     9421 aatgagatga tcaaacaaag cgagctaaat caaaaagatg tgggtttatt tacagaattg
     9481 atctatggta cgattagcca tcagcgacta ttggaatttt acgttgcacc attgatcgct
     9541 aaagcaaaaa aagtggatga ctgggtcaag acgcttcttt atctttctgt ataccaatta
     9601 gaatttttag ataaagtccc tgcacatgct attttaaacg aggcggtcga aatcgcaaaa
     9661 gtaaaaggaa atcctgggac agggaaattt gtaaatggtg tcttgaggaa ttatcagcgt
     9721 caaggagcac cagatctgca aggcattgct gatcctatcg aaagactttc tgtcaaaatc
     9781 agcctgcctc tatggatgac taaacgactg gtggatcaaa tcggatacga agaaactgaa
     9841 aagctcggtt tatcgctata tcagcctagc catgctagtg cccgagtaga tacacgccga
     9901 ctttccagag atcaggccat cactgttttg caagaagaag agatcacagc tgaaaaaagc
     9961 caagtcagtc catacggtat agtagcgaaa aaagggcatt tagcttctag ctcattgttc
    10021 cacgatggcg tcatgacgat ccaagatgaa agttcgatgc ttgtagcgcc gtctatgcaa
    10081 atcgagcctg aacatcatgt cttagatgcc tgtgcagcac ccggaggtaa gacgacgcat
    10141 attgcttctt ttttagatcc gtcatgcggt ggagaagtgg tcgctttaga tgtacacgaa
    10201 cacaaagtca aactcattga agataatgca gcacgtctag gtgtctcttc tgctgtctac
    10261 gctcaaaaga tggatgctag agaagtcagt aaacagttcg aagcagagca gtttgatcgg
    10321 attttagtag acgcgccttg ttcaggtctt gggctgcttc ggcgaaaacc agatattcgt
    10381 tataaaaaac agccggatga attagcgaat ttaccaaaaa tccaattgga aattttagaa
    10441 agctgcgcac caactttaaa gtcttccggt attctgactt atagcacttg tacgatctta
    10501 aaagaagaaa accaagacgt tgtttctgct ttcttggaaa gacaccctga ttttgagagg
    10561 attgatgttt tgaccaatcc tatgctacaa tcatcaatcg aagagaaaat gttgactttg
    10621 tatccgcatc agttttacac agatggattt ttcatttgtt gtttgcgcaa aaagtaatga
    10681 ggtgaaaaaa tgcagattga atatcaatca gatgtaggaa gacgacgtaa cacaaatcaa
    10741 gactatgcca gtgtatttac cagccgttca ggtctcaagc ttgctgtatt agcagacggg
    10801 atgggtggtc atcgtgccgg agacattgcc agtcagatgg cggtaacgaa tctaggcgaa
    10861 gcttgggaaa aacaagaagc tgttgacgat gaaaaaatcg cccaatggtt cattcaatcg
    10921 attcaagaag aaaatatgct gatttatcag cgtggacaag aacagccgga atataacggt
    10981 atgggaacaa caatcgtagc ggccgctctg tcagatgact ggttcacgat tgccaatgtt
    11041 ggggatagca gagcttattt gattcgtgaa gataaaatca tgcaattgac ggaggatcat
    11101 tctttagtca atgaactagt caaatcagga gaaatcagcg aagaaatggc agtgaaccat
    11161 ccaaggaaaa atattttaac acgttctgtc ggcatgcctg gaacagtcga agtagatgtt
    11221 tctactcatc tttggcaact aaaagatcga ctgctgttat gttcagatgg cttgacgaac
    11281 atgctgtctg aagaagaaat aggaacgatc atcaatcaag aaggcccatt gtctcataaa
    11341 gtaactgagc tgattgatcg tgccaatgaa gcaggcggag cggataatat caccgttctt
    11401 cttatcgaat ataaggagga cagtgtatga tcgagttggg caaaaaatta aatggccgtt
    11461 atcaaatcac tggaaatatc ggtagcggtg gtatggcaaa tgtatttttg gcgcacgatt
    11521 taatcttgga tcgtgatgta gcagttaaag tcttacgatt tgactttcaa aatgaccaga
    11581 ctgcgattcg acgatttcag agagaagctt tagcagcaac agaactcgtt catccaaaca
    11641 ttgttagtgt ttacgatgtt ggtgaagaag acaacatgca gtatctggtg atggaatacg
    11701 tcaaaggaat ggacttgaaa cgatatatcc aaacacatta tcctgttcct tatgaaacag
    11761 cagtaaatat tatgcagcag atcctttctg ctatttcttt agctcataac catcaaatca
    11821 tccaccgaga tttgaaaccc caaaacgtac tgatcgataa tgaaggtgtt gtaaaaatca
    11881 cggatttcgg gattgctatc gctttatctg aaacatcgat cacgcagacg aatacgatgc
    11941 taggatctgt tcattatctg tcccctgagc aggccagagg aagtatggca acgaagcagt
    12001 cagatattta tgctttaggg atcatccttt atgagatgtt gacgggtagc gtaccgtttg
    12061 atggtgaatc tgctgtaaca attgctttaa aacattttca agatgatctt ccatctataa
    12121 aagcacttga tccaaatgtg ccacaagcac tggaaaacgt gattttacgg gcaacggcta
    12181 aagaacctgc agaccgttat aagtcagccg aggaaatgtc tgatgatctt tctacagtat
    12241 tatcaccagc tcgtgcaaac gaagaaaaat ggcagcctca tgtgatggat aatgaaacaa
    12301 aagttatcac accccttacg gaagatgcac caatgcctga ttcattcaaa tctatgcctc
    12361 tgccaaaaga caagccagaa aacgaaacct taccagagga ggaagaagtt ctgcctgatc
    12421 ctaaaaagaa aggacagaaa aaatggtggc tgatcctatt agctattctt gctgttttag
    12481 gaatcggaac agctgtattc tttgcttctg gcgggcgagg agaagttgtt gttcctgatg
    12541 tttctgatct ttctgaagct tctgctagag aaacactcgc aaaagcgaaa ttagaagtag
    12601 caacaaaaac agaagaaatt gcagatgaca aaatcgaaga aggtaatgta gtgaagacag
    12661 acccggcagc gggaacgaca gtcaagcaaa aacgcgaagt cactctgtat attagtagcg
    12721 gcacgaaaaa aatcaaatta gatgattaca gtggtaaaag ttatgaagat gccgaagcaa
    12781 gtttgaaaga attagggttt tctgctgact tgattcaaaa agaagaggaa ttcagttcag
    12841 aggtagaaag cggaatgatc atttcgcaat ctccatcaga agatacagaa gtcgatccta
    12901 aaacagacac gattactttc acagtcagca aaggaccaga aacagtgact ttgagtagtc
    12961 atgtaggaga aaactacgaa actgttcgta attggcttgt tacacaagga ttcaatatca
    13021 ataatattag tgctacttat gactatagcg acactgtacc atcaggttcg atcatcagtc
    13081 aggatccagc ctcgggtgaa gttgtagcag aagaaactta catcaatttt gtcgtaagcc
    13141 aaggaacaga gcctaaattg aatgatatct ccggctatac aaaaagcgaa gcacaaagtt
    13201 atttagctag tgtcggtgca gaatatatcg ggcatgagac ttccgaatat tcaaacacgg
    13261 tggataaaga taaagtgatt cgtacagtac caggggcagg aacgacaatt accaaaggaa
    13321 tggttgtgaa cgtaatttac tctaaaggac cagatccagc gtcttctagt tctgaaaaag
    13381 actcttcaga cagttctagc agctcaagta gctccagtag cacaagcaac tcttcaaaag
    13441 aagaatcttc cagctccaca agttcatcag ctagcacctc agaaagttca aaataagtgc
    13501 tgaataaaat atatcagaag ggaatgcgac attgatttgt tgcattctct tttttgtgtt
    13561 tctatctctt ttgatcaatt ttcaagccat atactttgag aagtttgcat ggaataacac
    13621 ctactttcct cttcgtatgg taaaataaac aaaaacggag gtggcactta tcaaaggaca
    13681 aatcagaaaa gcaatcagcg gtttttacta tgtatacgca aacggagaga cctatcaaac
    13741 aagaggaaga ggaaatttcc ggaatcggaa aatcacgccg cttgtcggag atcaggtgat
    13801 ctttgagagt gaaaatcaaa cagacggcta tcttctggaa atcttgccga gaaaaaatca
    13861 attagttcgc ccaccagtca caaatgttga tcttggaatc attgtcacaa gtatcgtaga
    13921 gcctgcattt tcttataatc tacttgaccg ctttctagtt accttggaat acgagtcgat
    13981 cgaaccgatt atttttttga caaagaccga cttgtcagac gacccagaaa aaatcgaaga
    14041 gatcaagcaa acctatacta aaataggcta tccggtgatt gtacccaatc atacgggtga
    14101 tacgacagaa ctgatccgtt acttcccaga acggctatcg gtctttatgg gtcagtctgg
    14161 agcaggaaaa tcgacgctac tgaatcaaat atcaccggaa ctgaatttag caacaggaga
    14221 gatttctgat gcacttggaa gaggacggca tacaacacgt catgtggaac tgattcctat
    14281 ctatggcggg ttagtagcag atacaccagg atttagctcg attgactttt tgacaattga
    14341 agcaggagaa ctgccgagac aatttcctga atttgtagaa gcttctgtcc attgcaaatt
    14401 tagagagtgc atgcatgcta aagaacctgg ctgcgaagta aaacatagag tagaaacagg
    14461 tggtatcgca cagacacgtt acgacaatta tttgcaattc ttacaagaaa tcgaaaaaag
    14521 aaaaccaatg tatgcaaaaa aagaaggtaa acgaaaatag gaggaaacaa gaatgaagat
    14581 agcaccatcc attttgagtg cagattttgc caatttacaa agagacattg agttagtgga
    14641 aaaaggaggc gcagattaca tccacgtaga tgtaatggat ggccaatttg tcccaaatat
    14701 cacattaggg ccaaatattg ttcaggcaat ccgtcctgtt actaaattac cgttagacgt
    14761 ccatctgatg atcgttgatc cagaaaacta cattccagca tttgcaaaag ctggagcaga
    14821 tatcattact gtccacgtcg aagcaactcc tcatattcat cgggcacttc aaatgacaaa
    14881 aagcttagga gttaaagctg gagtagttat caatccagga acaccagtct ccatgatcaa
    14941 acatgttttg cctttggctg atcaagtact agtcatgact gtcaatcctg ggttcggcgg
    15001 acaaagcttt attgaagaaa cagtagaaaa aattacggaa ctcgctgatc tacgtagaga
    15061 gaacaattgg acttatgcaa tcgaagtaga tggcggaatc gttccagaaa cagcaaaaat
    15121 ctgtaaagat gcaggggcgg atgtcttcgt tgcaggatca tatatctatg gagccgaaaa
    15181 tccagtcgga caaatcgaac gattaaaaga ggcattagac taaatgaaaa ttttattagc
    15241 tgctggaggt aatccgcaaa actggccgca gtctctgctg tccaaatacg atgcctatgt
    15301 cgggatagac cgtggtgcgc tgtacctgct tcaagcaggc tacccagtag atctggcagt
    15361 gggcgatttt gattcattgt cgaacgaaga gcagaaggaa gtatttgcaa aagccaaaga
    15421 gtatacccgt tcccctgcag aaaaagacga tacagataca cagttaggta tcttaaaaac
    15481 atttgaacgt tacccgcaag cggaagtcac tttgattgga gctacgggcg gaagattgga
    15541 tcatctttta gccaatctct ggttaggtat ggaacctcgc ttcaaaccct ttgcccatca
    15601 gcttattatc agagattgtc aaaattattt gtcttattat ttgcctggag agcatatagt
    15661 aagacagata ccagatatga agtatttagc ttattgctgc ttgactcccg tctcgaatct
    15721 tacattaaaa ggcagcaagt atgtattgga aaataaagaa gtagaatatc cgacatctta
    15781 tgcaagtaac gaatttcttt cagataccgc agaattttct ttttcagatg gtatgattgc
    15841 agttattcaa agcaaagatt gatccataaa caaataaaaa aatttgtctt tcaggctaaa
    15901 aatgacagaa tctattattt gctaaaaaaa agcttaagag gagcaactta ccttttgttt
    15961 cacggcattt agtgataaaa ttgaacacaa gagtctaatt aattcgaggt gtctttgtga
    16021 agaagttcaa tcctaataaa aatattatta ttaccttggt tattgttatc atcgtggtga
    16081 cgatactaag tgtgaccgct gcgcagcgag cgaatgaagg aaagcccaat ttcttccaat
    16141 ccatcgttaa tgacagtgtt gcaatggttg ataaaacaat ctctgcccca gtacggtggg
    16201 tacgaaatgg agtagattct gtccataatc ttttttcaac ttatgccgaa aatgagcggc
    16261 taaaagaaaa aatcgatagc tatgatgacg tttcacagca aaacaagaat tacgagagag
    16321 aaatcgaagc gttgaaaaaa gaacttgagt tgaatcagac gctgactagt tacgaaaaag
    16381 taacggcaaa tgtcattact cgttcgcctg acagttggca ggacatgctg atcgtagaca
    16441 agggttctaa agacggtatc gaagcaaata tggcagtcat gtcgcaaaaa ggattgattg
    16501 gccgagtgat cgaggtcaat acggcttcgt ctaaaatcga attactgtca tcctctaatg
    16561 aaagctccaa tcattttcca gtacgggtat cttcggctaa tggcgaagcg tttggtttgc
    16621 ttaaaaacta tgatgaaaag ctccatgcct tagtggtgac ccaattaact ggtgatacgg
    16681 atatcaaaga aggggatgtt gtccagacat ccggtcttgg agggaattct ccagctaact
    16741 tgccgatcgg tacggttatt aaaacgaaac cagatagtta tgggctggat cgggaagttt
    16801 atgtgaaacc ttatgcagaa atgtatgacg tgtcagttgt gacgattgtc caacggctag
    16861 cggaggatta gtccatgttg aagaaagaaa caatgaagta ctatctgcca atcgttttgt
    16921 tctttttgat gttgatagat ggtcatttaa caagaatgct aggggagtgg tcgaaaggca
    16981 cctatatgtc aaatgcccac tttctgatat tggcattatt atgttgcagt atggcgtttg
    17041 aaaaacgtta tttactgatt accacgattg ttctcggggc tatctatgac gcttactata
    17101 ttggcgttat cggtatctat gcagtagctc tccctttaat tgtatggttg atgtatgtaa
    17161 tgaaagacgt tatccatgtc aacatcttta ctgaattttt cagtatgatc atctttgtca
    17221 cgggttatga attgtttacg atggtggtcc agttgatttt taaattagca gtagtaaata
    17281 acacgtattt tattacaagg tttttaggac ctacactgct gt
//
LOCUS       DAXWOO010000045        17098 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.045, whole genome shotgun sequence.
ACCESSION   DAXWOO010000045 DAXWOO010000000
VERSION     DAXWOO010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17098
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.045"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(36..581)
                     /locus_tag="ACGE70_001223"
     CDS             complement(36..581)
                     /locus_tag="ACGE70_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFF8692872.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(593..1285)
                     /locus_tag="ACGE70_001224"
     CDS             complement(593..1285)
                     /locus_tag="ACGE70_001224"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFF8692873.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1304..1588)
                     /gene="macP"
                     /locus_tag="ACGE70_001225"
     CDS             complement(1304..1588)
                     /gene="macP"
                     /locus_tag="ACGE70_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFF8692874.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1601..2164)
                     /locus_tag="ACGE70_001226"
     CDS             complement(1601..2164)
                     /locus_tag="ACGE70_001226"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFF8692875.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2392..3054
                     /locus_tag="ACGE70_001227"
     CDS             2392..3054
                     /locus_tag="ACGE70_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFF8692876.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3178..4374
                     /locus_tag="ACGE70_001228"
     CDS             3178..4374
                     /locus_tag="ACGE70_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFF8692877.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4475..5851)
                     /locus_tag="ACGE70_001229"
     CDS             complement(4475..5851)
                     /locus_tag="ACGE70_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFF8692878.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5888..6754)
                     /locus_tag="ACGE70_001230"
     CDS             complement(5888..6754)
                     /locus_tag="ACGE70_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFF8692879.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6843..7868)
                     /locus_tag="ACGE70_001231"
     CDS             complement(6843..7868)
                     /locus_tag="ACGE70_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFF8692880.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7886..8560)
                     /locus_tag="ACGE70_001232"
     CDS             complement(7886..8560)
                     /locus_tag="ACGE70_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFF8692881.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8744..9637)
                     /locus_tag="ACGE70_001233"
     CDS             complement(8744..9637)
                     /locus_tag="ACGE70_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFF8692882.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9711..11408)
                     /locus_tag="ACGE70_001234"
     CDS             complement(9711..11408)
                     /locus_tag="ACGE70_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFF8692883.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11401..12714)
                     /locus_tag="ACGE70_001235"
     CDS             complement(11401..12714)
                     /locus_tag="ACGE70_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFF8692884.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12714..16983)
                     /locus_tag="ACGE70_001236"
                     /pseudo
     CDS             complement(12714..16983)
                     /locus_tag="ACGE70_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333417.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
BASE COUNT         4813 a         3845 c         2956 g         5484 t
ORIGIN      
        1 ttgtgctttt tattttatca aatcactttg tttttttata aaatttctgc gcctagtgtg
       61 gactcaaaat gtctcaatgc ccattcatgt ccaacgggat cgaaacttgc cacggcatct
      121 ttgaaaatat ggagtttgta tcctagatta tacgcatcga ctgctgtatg caacacacag
      181 atatctgtac atacgccggt caaataaata tctgtgatct ggcgttctcg caaacggatg
      241 tctagatctg ttccactaaa agcggaataa tgccgtttgt ctatccaata aacattttcc
      301 tcttgctcat agtcttggta taaacttttt aaagagccgt acaattcccg tccacccgtt
      361 cctatcacat tatgcggagg aaataagcgg ttctccggat gaaaggaatc ttttggatca
      421 tgcgcatcaa tggcaaaaac gacaaaatcc ttttgatcaa taaacttctt tgtatgagca
      481 gtcagctttt tttcaattac ttgtcctgct gtcccagtag tcagctttcc atcctctgca
      541 acaaaatcat atgtgtagtc aatcgagatc aatgcgttca tcttagtcct ccttatacga
      601 gatgtttgac aaattcgatc accatttctg ctgatcgttt gcctgcgtct tcgataaatt
      661 cgtcaaagct ttgtgtcgcc gagtgatctg ctgtgtcgct cattgcacgt acgatcaaaa
      721 atgggatatt gaactgacga gctgtttgtc caacagcggc tccttccatt tcgcaagcta
      781 atgcttcagg gaaattcgtt aagatttctt tgattttatc tggcgagtcg acaaatgtat
      841 cacctgtaac gatcaatcct tcttttgcgt tcaaattcgt tgcttttgct gctttaacca
      901 tttcactgcg caaatatgtg cttgcttcat aatatagagg cattcctggt aattgtcctg
      961 ggttgtagcc aaatcctgtt gcatctgcat caaaataagc tactttgtct gatataacga
     1021 tgtcacctac ttgtaagcct tcaccgattc cgccagctga acctgtattg atcaccatgt
     1081 tcacgccgta ttgttggatc aacagacttg tggtaataga tgctaagact tttcctatac
     1141 cggaacgtac gacgatcact tcgtggttac ctaatgaacc tgaaataaat aaagcgcctg
     1201 cgcgttccca tgataatggt tcgcttaggt tctctctcaa aatttttact tcttcttcca
     1261 ttgcgccgat aataccgatt ttcatctttt taattccttc tttctatata aatgcgattg
     1321 ctaaaaaaac cacacataac attaggataa caataataag agatttcatc aaaaaagagt
     1381 tccatttggt cgtcttttcc cgttcgctga tccttgtttt cgtgattggt ttattctttt
     1441 tgctttcttt acgataaaag ctggcaatct ttttttcttc ttgctgataa gctgtttctg
     1501 cttttcgctg tttttttaat gactcgcttg cttgtgcttg ttgtcgcttg cgaagttcgc
     1561 ttcgcgtaat aagcggtcct ttggccataa gccgatccct ctactttcct ttggtcaata
     1621 gttcccagta ttgaatcgca taaatcgtct ttgcatcaca aatcaactga tctttcatcg
     1681 cttgttgcgc ctcttcaaga gttagatgat acaactccag tacttcgtct tcatcttgtg
     1741 ctaaaggatt ctcgaccttc tctacccctt gtgcatgata aatatgaagg acttcatttg
     1801 caaaacctgg tgaaagatac atcgaagtaa gatggctcag cgacttcgca cgatagccag
     1861 tttcttcttc taattctcta gctgctgtca tttcgggatt ttgtccttct cctggatcga
     1921 tttttccagc tggaatctcc aaaatcactt tttctaaagg ttttcgaaat tgtttgacaa
     1981 gaagcagacg atcttgttcg tcaaaagcaa tgatcccgac accgccagga tgaaatacca
     2041 gttctctctt cgaagttcct ccatctggca aacgaacttc gtctacagca acatcaataa
     2101 tttttccgct atagatttct ttgcgactga ttgttttttc ttcaaattgc tcattgttca
     2161 gcatatcttc tcctgctttc tttgtaatca taactgcttt tttttcattt ttcaattttt
     2221 ctcaaaaact ccttattagt ttaccataga taaaccttag attttctcta aatatagaaa
     2281 atccttcttt agtccgatac tctttatagt agtccgtatg ctaaaataag tatgtaatcg
     2341 aataacgaaa agaattcaat actcaacgat aaatgacatg gagggtttat tatgataaaa
     2401 cgaaattggc cgtggcttct agcattggtc ttgctatttc tctttgtccg aaacaatagt
     2461 ttttcttttg ttctaggact tttattgata ggtgtagttt tagttttgtg gggactcttt
     2521 ggttcgcgca ggaaaaaggg aaaacaagaa gaaattcctg cactgactga cgaacttgaa
     2581 actcactatg aaaaaagcgg tatgacagcg aatgaaattt cattttttcg acaaacaatg
     2641 aatcaaacaa aaaatgagat caatcaattg caaatgaata tgcagcagac agccaaactt
     2701 aaagcaatcg atctccgcca tgatccagtc aaagcagcga aagctttatt caaagaattg
     2761 gtgaaagaac caaaccgact gcatgaagca agtcagtttc tttatacgca tctgccgaat
     2821 ctagttgatt tgacaaataa gtataatgag atcaatgacc atgagatcaa aaacaaacag
     2881 acctatgaaa aactagaaga aagcgcgcag atcatcgacc agctgtcagc tttaatcgca
     2941 caagattatc aaaaattcgt tgctgaggat ctagatgata tcgacgtaga gatatctgtt
     3001 gcgaaacaga gcttaaagcg tgataataaa tatgaaccag aacaaaagga ttaactcttt
     3061 tgttcataaa agaagactcg gaacattcag atgatgtctg gctgtttcga gtactttttc
     3121 taaagtagag acattcatgt aagaatcgag aacagattca acaaggagga attttttatg
     3181 gatcataaac cgactgaaac aaaagatgtt acaccagtta acgatacact tgaagattta
     3241 ttgaacaacc cgttttcaac tccaacagat acgctgacca cttcacaaca agcggagatt
     3301 gatcagctcc aagagcaaca gacagctgct cgtttaattg acaaactacc ggcggaacgc
     3361 caagaacaag ctaaacagct agcagcaaaa atcgatgcta atgatgcaca gtctgtcatc
     3421 agctacggat cggctgccca agcaaaatta agtgaattct cacaatcgat gttgaatcat
     3481 gtgcaagcac aagatattgg tcctgtagga gattccttaa ctgaactgat gtatcgtctc
     3541 caagaagcca atcctgacga actgcgtgcc ggagaaggga acatcttcca gcgtgtcttc
     3601 gggaaggtca aacaatcgat ctatgaagtg acagcaaaat atcaaaagat tggtgcacaa
     3661 attgacaaga tttcagtgaa attagataaa gaaaaagacg gtctgctaaa agataatctg
     3721 atgttagaac agctttacca gaaaaacaaa gactactttg atgcactgaa tatttatatc
     3781 gctgctggag aattgaaaat ggaagaactt caaacaacga tcattccaga agcgatgaaa
     3841 agagcagaag aaactggcga tcagatggat gttcagattg ccaatgatta cacgcaattc
     3901 ctagatcgtc tggataaacg tacgcatgat cttcgcttag ctagacagat cacgatccag
     3961 caagcaccac aaatccgttt gatccaaaac acaaatcagg ccttggctga aaagatccaa
     4021 gcatctatcg ctacagccat tccgctgtgg aaaaaccaag tcgtgatcgc attgaccttg
     4081 cttcgtcaaa aagatgcagt aactgctcaa cgccaagtct ctgaaacaac aaacgactta
     4141 ttgaagaaaa attcagaaat gctgaaaatc tcagccatcg aaacagcgaa agaaaatgaa
     4201 cgtggaatcg tggatatcga gacccttcaa accactcaaa atgatttgat cgaaacaatc
     4261 caagagacat tgcgtatcca aaaagaagga aaagaaaaac gtcgtcatgc agaagtagag
     4321 cttggacaca tggaagaaga tctgaaacaa aaactgttgg atttaaccca atagatcacc
     4381 tttcgcttgt tactaaaaca aacgcccaac aacagtttat tacaactgtt gatgggcgtt
     4441 tttcttatct ttcttctaac ttctccattg gagtctattt ctcattcatc ttgctgtgtc
     4501 catagccata gccaaaataa atgatcagtc caataaccat tacgataccg aacacgatcc
     4561 atgtcacagt ttgcagacga agcatcaata caatacaagt caagatggag acgattggca
     4621 atagcggaac gaatggcact ttgaattctc cagcttttgg tttgccaaac atttttctca
     4681 acttcagtaa gcctagacta acaagcatca aataggcgag cgccatgata ttcgttagtt
     4741 ccgctaatac attcaatggg aaaaatccgg caaagaatac agctgccaca ccagcggcat
     4801 aagtcgcttt tttcggtgta cgatgctttt gatcgatttc tgtcataaac ttaggaagta
     4861 agccatcctt gctgacagca aacagtaaac gagcaagtga atacatcatc gagatcgtta
     4921 ccgtcagtag cgttaaaatt gcccctacag cgatcaccgc gcccactgtt ccatgtccta
     4981 catacctcat tgcaaaagcc actgggtcat tgactcctag ttcagtatac ggaacgattc
     5041 ctgtcaagat cagcgtcacg atgatgtaaa ggacagtagc aatcagtatc gaaccaatga
     5101 ttcctcttgg cacatctttt tgcgggtttt tgacttcctc ggctgccatt gcaacggcat
     5161 caaatcccaa gaaagcaaaa aacacaagag ctgcaccatc tagcacaccg gaaaaaccaa
     5221 aaggcatgaa tggctgccag ttatcaggtt taacgaaaaa tattcctaca atgataaaca
     5281 acgcgatgat accaaatttg acatatacca tggcattatt caaacgtaat gctttcttgg
     5341 cttcaagcga cacgatgtaa gtgacgaata tcactacgag caccgcaatc aagtcaatgt
     5401 atgtaccatt ttccggattg tacccgccag ttaatgcttg tggaagttcg gcccctaaac
     5461 ttttcaaaaa accttgcata tagccagacc atccggaagc aacagaggaa accgctagta
     5521 aaaattcccc gatcaacaac caccctgtca gccaggcagc aaattcacca aatacaacat
     5581 acagataggc atatggccca ccaatcactg gcactcgcga agcaaattct gcgaaactta
     5641 gtccagacaa aatgacaacg attgcagcta agacaaatga aagtgacagt gctggaccag
     5701 cattttgatt ggccgcaacc cctgtcacca caaatattcc cgtaccgatg atcgcaccaa
     5761 ttccaagcat gatcaaatca gacgttttta gttctttttt catctcactc gtatgattga
     5821 gattgatctc tttcttacga aacaaatcca ttgaagttcc tccccatttt aggttggaaa
     5881 acagcgatta aactgttttc atatacccta aaaggatacc acctaaaatg actaaaaggc
     5941 aaccaacaat aacataaatg agctcttttt tcgtttttct ttcgccaagg atgaaaatcc
     6001 cacctaaagt agagataatg atccccattt gtgaaagtga gaagccaaca gctaaaccaa
     6061 gtgatttgac tgttagaagc atgcagatgt ttcctaagcc ccataataat ccacagatca
     6121 tgtttttcca cacaaactga tcgactttaa ctttacggaa agcaaagaag cttgcgccta
     6181 agatcattcc gatactttga ggtaagataa ttgacatcgc atccaaattc gcccagttga
     6241 cgatgatggt atatacacca taaccaatcg tagagaagat caatgctcta aaacccttac
     6301 caaaatcaag cattttatta tctgtatcta tttttccttc gtcatcttgt cgggcagtca
     6361 aatacgcacc gcttacaaga agagctaaag caataatacc gtatacataa tctttcgttt
     6421 gtgtccattc atggaagaaa acggctcccg caatcgtatt caaaattaat tgaaaaccag
     6481 tagacaatgg tagccctaca gaaacaccca tgtacttcat tccatggaac tgtccatttt
     6541 gtccaacact ccaagctaag ccggataaaa atccgatcaa gaaaatccaa ggggtgatta
     6601 caggactaac aacgaaaaag acaactaaag aaaacagaaa tgcaccgatc gtcattccta
     6661 gtgtttgttg attggcatcg ccaccaattt taccacttac taaaccaata ctaccccatg
     6721 ccaacatagg gactaatgct attaaaattt ccataaccaa tctcctttta tttcacatga
     6781 taagaaactt atcagcgaaa acgttaacaa cacaagcact ttttttcgtt tctaacgatt
     6841 ttctaacgtt tttcattata aaaatttaag attgggaaaa ataaagtcat tgttgtccct
     6901 tttccttcag ttgattcagc cgtcaaatgg atacctaatt tttcggccag attttttgct
     6961 aaataaagtc ctaatcctgt tgaatgtgtt ttgctaagtc gaccatttct cccagtaaat
     7021 cccttatcaa agatccgttt caagtcttcc gctgggatac cgatccctga atctttaact
     7081 gaaaggctga ctccttgcgc ttccttttct atgctcacaa tgatgtttcc gtgatccggc
     7141 gtatatttga tggcattact aagcaattgg ttgaagataa aagcgatcca tttagcatcg
     7201 gtcaaaacag tatgatcctc accaacgatc tcgtagcgaa tatttttttg gataaaataa
     7261 tttgcctgac tgcgtatcac aggctgaatg attcctttta gtccatattc ttggatgagg
     7321 tagtcccgcg agaaactgtc gagtcttgca taatacaaaa cttgttctac gtattcatcg
     7381 atttttgata attcattttc tagtaatgta tacttttgat cgtcaatatc gaactcgatc
     7441 gaacgaacta gcaatttaga agcggaaagt ggcactttga tttcatggac ccatgagtca
     7501 atataatctt tttggtcttg ttgattactg attgctgatt gcattacttc ttggtgttca
     7561 cggatcagct gattgatata ttcttctacc agtttttctt cttctgtcct cccaccactc
     7621 aaataatttt gcaaagggga agttttttgt atgtcggcta attttcccca ccatcttctc
     7681 ttgctcaagt aaccacccag cagataaaaa atcagaaaga cgccctcaat caaagctaaa
     7741 taagcaattg tgctcaaatc aactttgatg tttggcgcca gccacatcac aaagatcgtc
     7801 aatgcaataa aaaacagcca tcccaagatc aatgtccact gatctttcaa atatttaaaa
     7861 aaattcatct taaccgctca tcctttcagg gaacgatata tccctgtcct acttttgttt
     7921 cgatatatcc ttggatacct gcctgttcga tttttcggcg taaccgattg atgttgactg
     7981 tcaatgtatt gtcatctaca aatcgttcgt catcccacag tgctcgcagc aatttttccc
     8041 gacttaagat ctttccattg tgcttcatca aaataaataa tagacgatat tcatttttgc
     8101 tcaaatcaat gatctctccg ttgatttcca tacttccgtt gtcaacattc aacgtaatgc
     8161 cattgtggct catcatttca ctagataatt ctgtgtagtt gtaagaacgg cgaagtaatg
     8221 cattgatttt tgcaatcagt acatcgattt ggaaatgttt tgtcacaaaa tcatctgccc
     8281 ccatgttcat agccatgatc atatccatat tcgtattcct gctggagata aaaatgatcg
     8341 gtactttcga tacttcacgt attttttgac accaataata gccatccaaa acgggaagat
     8401 taatatctag taatacaagc tgtggttctt ctctttcaaa gtctgagaac acttgattga
     8461 aatcggtcgt tccaaaggtt tcaaattgcc atttctcaag ttcctccatg atcaattgtc
     8521 gaatgatctc ttcgtcttcg actaccatga tttttgccat aaaaaatccc tccatctttc
     8581 ttcttgctac tacaatatca aagaagccct tgtgaaacaa gttggcttac ctggaaaatg
     8641 tcattttagc aaacgttaaa aaaaactgga aatatgcgat ttaatcgcgt ttgtctcttc
     8701 caagcagctt aaatttatta tgttattctt aataattggt ctattatctg ttatttttct
     8761 ttcgttgttt ttgtctttta aattttcgat aacaccaata accgaaaagc gttagcacta
     8821 gcaatatacc tagaaggagt ataagtaatc gataaagatg gtattttttt gtctgttcta
     8881 tctttgaaga gacttcttct gtaaagggaa ctcgtcttcc tgtcactagt aatcggtgag
     8941 tgttcacggt gtaaggtgta caagtgataa gtgtgactag atcctgcttt tctctaattg
     9001 ctaatgtatt tgtttttgtt ggaaggacaa caattttctc aacgacttca taagcgagca
     9061 tccgaccata cacttcaaga tagaatcggt catcttgctc catttcttca agatccgtaa
     9121 agagtttttt ctcagcaata ccgctatggg cggtaatcac ggaatgtgta ctctctcctc
     9181 cagttggaaa agaggtgcct tgcaggactg tcgctcctct atctaacaaa agatcattcg
     9241 tttcatcaaa taaaggcaag ctaacattga ttttcggaat atagatggct ccgatcatat
     9301 gttgttcgta atattctttt cctggatttt ggacatttct aaccgcttgc tcaaaaggat
     9361 ctttgacttg tcccattcct gggattactg atgtatgagc aagcgtctga tttttctttt
     9421 catgcactgc taattgttgc tcatgttttt cttctttatc gcgttcaagc tgcttttgat
     9481 aattctcgat ccttctttga tccaaataat tgctgagagc atctgctaaa aatggatatg
     9541 taaaaactgc agctccggca agaaacatca ctgccataaa taattgtaat aaaagactga
     9601 tttgggtttg aggtctatta cctattttct ttttcatttt cttcaccttc ttatttaaaa
     9661 aaactgcagc aacctttata aaaccgattg ctgcagctta tttttgttgc ttatacttca
     9721 gacttcattt tagatttacg gtaccagctg tacgctccaa tcatcaaact tattccgatt
     9781 agtaagaatg ccaagagccc gttcccacca gtagcaggaa gtgacccgcg tttcatattc
     9841 ggtactcttt ctggccctgg attttctact cctacgatct gaatttcttc attatagctt
     9901 ccataagaga tgataaattc agtaaaggct gcctcacctg ttggaagtac ataaccttct
     9961 ggcgccatgg tctctttcat ttgataggtt ccataatcta acccttgaac ctcaaagtat
    10021 ccatttccat ctgcgacaaa ttctgttgct tgtccgcttg ttgcccactc aattctatct
    10081 gctcctgatt cataaacagc taaaggcgac tcccctttat aaaatattgc gtattcagaa
    10141 cggtcttcat tccacaattc gaatcttgca tctttaagca attcactgct tgatgcatca
    10201 tatttttcaa actttcttcc acctgttgtg actttctctg gcggctctac tccttcttca
    10261 taatcaaatt catcatttcg tccgatttca ataaccgctg tgttgttatg gaaatcatcc
    10321 gcttctagtt ctgtggaaac cttcaaatta tacgtgatcg ttaaatctcc tccacgtgca
    10381 tctgctaatc tacctaaagt atctactttg gctactccat tttcttctgt caaaatcgtc
    10441 acgacaaacc cgttgcttac tacttctact atataatctt gtccttttgt taaaatcgtc
    10501 tcgccggctc gcacttctat ttcctgatca taataagtta gaccaactgt aggcgtatct
    10561 gtgattttgt aatttttgat aacagcgctt ccgttttcaa ggacagaatc gatgaaataa
    10621 ggaataggtg ctgtgatcgt gtaagaagcc gtttttcctc tttctagatc aatataagaa
    10681 atcgtttctt gttctccagc ttcattaata atcgttactt gtctaaggtc gctttcctct
    10741 acattcatct gtttatctgc ttctctgatt tcgtttttcg gatacaaatg cacatctgta
    10801 ttgtatgtgt ctttccatgt agaaccatcc cacacgccat ccaccatatc aggcatcatc
    10861 actggcatca taacaaccat tggagacgcg attctggaaa tatgagctgg cgaataagtt
    10921 tctaagaaaa gatagacttt atctctagaa gaaggtgctt cttccgtcac ttggacggta
    10981 gagaataccg cttctccagc ttcattcgtt gtttgtttgt caatacttgt cagtcgatat
    11041 tttaaaaacc aactttctgt gtgccgtttt tgaatccatt caatcgcttc ggcagaatta
    11101 agaccgtctt ctcgttccgg tgttaaagga ttatctcgaa tcaaatcgta atatacttcc
    11161 gttacgtcaa acaaatcaaa atcgactcct ggaatatttt cttcaccaaa atccgtagaa
    11221 accaatccgc tattcggtcg ttcttccgga acagatgaga aacctttttt gtgtaatgta
    11281 atcgtcactt cacttggttt ttgtgcgact tcttcggctt tgactagttt tccgttcccg
    11341 aaaatcccta ttcccgcagt aacgagtaac agtacactca ttcccgtgac aatcattttt
    11401 ttattcattt ttttctcccc gttctttttt tattaataaa acagcaccta acaataaaag
    11461 aattcctagt aaagaaatca acgttcttgc ttcattcgtt tgcgggaata cggaccattt
    11521 tcctgattga cctggattgc ttggaccact cggctctttc ggtttgtccg ttgtttcttt
    11581 tacggtctcg ttataaacac gtggtgttgc ggattcatag gcagagactg gaactttccc
    11641 ggattctaat tcagccatct cctgtccgtt aactactaca tattgtggtt ggccttttgc
    11701 atccacccaa aattcaggga tgatgacctc gattctctta cttgcttcat ccatctcgta
    11761 accttccaca ccttttaatt cttcaaaata ataggtgcca gaaggcaaga aacgttgtcc
    11821 tgtcgtaaca aggccctctt tatccgaaat gaatttgctt acatttttgt cattcaaagg
    11881 atcattgtct gctggtttga tccattgatt tttcaagtca ttagcaggag ccatatctaa
    11941 ataatatttc cccccttcta tcatctgata tagagcaaaa acagcacctt ctaatggttt
    12001 tcctttttca gttgttcctc tttgacgtcc atacttgaag aagtatggat ctcttagata
    12061 accgacattt ttaggataga tatgaatctc ttgcagttcc tcggttgatt ctgtcgggtg
    12121 ataaattggc aaaatagcag caattgggat agctgtctga tcgatatcaa catccaatcc
    12181 aacttctgga tcaatcgatt cttcgaaaat caaataaact gcatcccgat cttggttttt
    12241 cttaggtgct ttgatacgtg caatcccgtc tcctaatccg ccaaccgtat ctttcccagt
    12301 tgttaccgaa gtccctattt ggacaagcct ttcttctgtg attagctgac ggatattgtt
    12361 tctgttcgtt tcagcgattt gctggacgaa ttctttagga gtcatgtttt cagcagcttt
    12421 tttttcatga taataatcgg tcatgtcgta aatggcaaat gtcgctccat ttagtggcac
    12481 cgtctgtgaa atgatatctg ctgtttcccc tgcttcctca gagatagcca agccatcatt
    12541 ttcatggtat tcaaattgat cgttataatc gatatctcga atcatccgtt tatgcaacac
    12601 gaattccacg acatcttccc ctgattcttc tgccgacatc gcatgtggaa gaaggactgc
    12661 tgcaagcggt agtaaaacaa gtaattgaag catcaaacac aacagctttt tcattacctt
    12721 gctccctcct ttttagattg tctgacaaac aaaccatagg ctgctcctgc aagcgccatg
    12781 aagaatgcag caagcaggat atagaataat cgtccggatc ctcctgtagc aggcaacgga
    12841 ttttcaggat cgttcccgac actaagctgt aatctgattc gatttgtctc caaaagttca
    12901 tattctaact cttgctgtag atcaggcgtc tctgcttctt cgtttggata atacgaaatc
    12961 tcaactttta gtgtacccgc ttcttttcct ggaactaccc gatcattttc tatgtcaggt
    13021 tcttctcgat aactaatttc taaaataaag taacctggaa gttttctata gctgtcaggt
    13081 gacttcactt ccttgatccg atagacccct tccaacaagt ctgaagcatt gaatagggaa
    13141 gaatcgtttg gatctggtgc caagcctccc tcgatgattt catactctcc tgcactattt
    13201 tgtttctcaa ttgaaaattc tgcgccttca agacgtcggc tattcatact gtcaattttc
    13261 aaaagttcca aatcgattgg cgctagttca tcttctaagg ttacttgtag tacaccatcg
    13321 atgatttcat agccttccgg caagggagca gtttcgtctt cgatgaccgt atcttcaatg
    13381 ataaacttac cttcttcatt cagtgcaaac tggaacactt gatcagatag ccgataccca
    13441 tttggtgcga cagattccga gacctcatat tcaccaatag acagatgcag aggttggtcg
    13501 taatttaaat cgacatactt ttctactcct acggcacgag aatagaagta tccgatccca
    13561 ggcaaacgtt tcgattcttc tggttctaac ggattaccag tgtatgctcg atacagcggg
    13621 aaggaagaat cccccacttt cctgatatcg aaacctgcac catctaatgt atggccttca
    13681 tggtctaatt ttctaactcg gatctcccga taatcaaacg tattgtaagc agtgaatgct
    13741 acccataaac gatcctcttc atctgttgta tacgtgagat attgccaatt ttcttcacct
    13801 gtttgcttga gttggatagt ccctatagca ttatcgtaaa gtcgcaagat ccatgtgtca
    13861 ttttcatcag gttctttgaa accttctggg ttctggctct caatgatttt gtattctgca
    13921 tagttggtag tttcttcact atccttataa acaaatgaat ctggctctcc gattggatga
    13981 ataactttct caccatccac gattacatat ttcccgtctt catctactga atagaacgtg
    14041 agtctccctt tctcttcctc tacttcgttc acaccagaaa gccctttcgc tactggatat
    14101 ccgatttcca gtccgttacc atctgtttca taaagttcaa actctgcgcc attgattggc
    14161 atattactgc cttcaattag tttattgata ttcatgtgga ttggcttgaa ttcatttcgc
    14221 atttccagtt tgacatacaa ctcatcatac tctgtgcctt ctatgttagg ctcttgtaca
    14281 acagaaagct tgtgatattc tccttcataa taggaattcg gatctttacc ttccggtccg
    14341 ccgactctcg aaccagatgt atattcatct acaagctcgt agtgacctaa catctcatct
    14401 tcgttggctg catatttttt gatttggaat tgatacgtct catctactaa ctgataacgt
    14461 ggtggtccag cgatctctct gatccaaaat atgtcgctga ctgctccagt ttcatctcct
    14521 aaaatcggca tcttcgtttc aaagatcgct attccgtccg tggtagtttt acttgtttcg
    14581 attggttcgg cttccccact ctctggattt tggtcatata atgcaaattc gactccagcc
    14641 aacggctcgc cattttgacc cttcttgctg acttggaaat tacggccttt atgttcgtaa
    14701 cgaatggaag aagatgggaa atacatccgt ccagtagtga tatcattctc ataatcctcc
    14761 aggttctgaa tataggccat attgttcact gtgcaaaaat ttccgtcttg agctcccgca
    14821 tcaagtttta cttggtactt gatcctgacc atttcccctt cacctaaaga aatcggagaa
    14881 aatttataag cttggttttc ccgtccgaca tacgttgtcg gagtcgccac tacttctggt
    14941 ggggcttgtc catgacgttc ttcatcccat atttctggct cttggtactt catgccgttt
    15001 tctcctggat ctagatggta aaactgaata tctgccgatc tgccagctcc aaatggtgag
    15061 acattaaaac cagtttcatc ataaagtgtg gcttgctgga tcgttctctt gaaggtagaa
    15121 gagatatcct ccaaggctgt aacaatgtca ttcttgttgt tcgcattgta atatcgcggt
    15181 gttacgccat cttcttctaa atcagcaata tttttcaata catttcgtcc aatagcatct
    15241 agtcgttgtc tcccgctaac gtttccatcg attcctaaac caatggagaa gatacttggc
    15301 gggttctctt gatacttgtt tttcagccaa tgatgataag caactgtgtt gatcgcagat
    15361 gacttttctt gcgaagtagg actgccattc gtattgttat tagaagaacc atcaccaacc
    15421 cagtgagtcg ctttattatc tccttctttt acctctccat aacgatagtg tacatttact
    15481 tcatcttctg gacggttaaa gtcagatgca taggtaactg gatgagtaaa ccctgcatta
    15541 gaagtattcc cagaaaactc ctcaaaattt ctgaacaaaa cgtcattatc ttcagcaatc
    15601 ttatcttcca tcacagtcca gttgctccaa gctccattag tgctcgtacg ataacttgac
    15661 tgaatcggtg cataactgaa ggtcggtgtc ccatctccca caacgattag gattttttct
    15721 ggatttctat tttctccgcc attgtcttta tacaatgttt catagccatt tttaagccct
    15781 agtgtaagcg gagttcctcc gattggtgta cgatttaatg ctgtattctc acttcccgtc
    15841 aaacgattga tatcatttgt caatggaaag atgtccgaac tgatttgctc atggttattg
    15901 atagctgtac tattatggta aaaattcacc ataccgattc gaatatccag actcgggtca
    15961 gagagcagat ctttactcat ttcgtttact gcctcaatca acgcagcatt tttagtttga
    16021 ctattggtcc cagcggttaa ttcactcata ctcgctgact tatctagaac aaaaacgata
    16081 tcgattggac tgatttcctt tttctcgccc ccgatgacat ctagatagac attgtaaagg
    16141 gttgttgcat cttcaggatg tagatcagat gctgtgctgc ttggcagaac aggttctacc
    16201 cattttttga tgtacgcccc gttataatcc aagtaactgt tttcaaaatt ccgttcattt
    16261 ccccaaagct gaacggtttg agctggaacg tttctatctt cttcgcctgc agttgatcca
    16321 ttattagtag catagtcgta atttcgccag ttcgctcccc cgtttgtata atcactatca
    16381 tcgatcaagt tctctccgtc atatggcgag cctttgtctc cttgatagcg ttcattgctt
    16441 tgtgtccaat ttcctaacac ttttgtatag tcattcgttg taaacttcat ctattctttc
    16501 atcaaccacc acttgaagag gtagttaccc ttcgttcaca tcggggatct tttctgtttc
    16561 aaaaatcaat gtgccggttt ctgcttcttt tgaataaact tttccgacat accattcttg
    16621 accatcgatc gtctcgactt ttaggtcttc tccatcttca tcataggagg atagacctgt
    16681 tccacgaacg ttttttacgg taccaatccc ggcggctgcc gcatcgattg ctaaacgtat
    16741 tctaccttca ttggcacttt cctttttttc aaaagccaga tgccattcaa gggtttcttc
    16801 tttttcaata tacttccctt caatttttgc atagtcctca tctaagaata cctcatcctc
    16861 ctttgtttct gttgactcag cagctacatc cagggtcgtc atatgataga ctgactggaa
    16921 atatggtcca aatagcaaac tgacagttac taactgcaaa agcttcaatt ttaacccttt
    16981 cacatcctta tactcctttc aaaaatattt gtcttctttc acaacaatca acaaaacaat
    17041 gcccatctaa ttataattta atttaatttt aattttaaat caaatgatat ccttttta
//
LOCUS       DAXWOO010000046        16872 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.046, whole genome shotgun sequence.
ACCESSION   DAXWOO010000046 DAXWOO010000000
VERSION     DAXWOO010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16872
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.046"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(<1..528)
                     /locus_tag="ACGE70_001237"
     CDS             complement(<1..528)
                     /locus_tag="ACGE70_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFF8692885.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKI"
     gene            complement(776..1612)
                     /locus_tag="ACGE70_001238"
     CDS             complement(776..1612)
                     /locus_tag="ACGE70_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFF8692886.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(1623..2552)
                     /locus_tag="ACGE70_001239"
     CDS             complement(1623..2552)
                     /locus_tag="ACGE70_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFF8692887.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            2744..3097
                     /locus_tag="ACGE70_001240"
     CDS             2744..3097
                     /locus_tag="ACGE70_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFF8692888.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(3171..3827)
                     /locus_tag="ACGE70_001241"
     CDS             complement(3171..3827)
                     /locus_tag="ACGE70_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFF8692889.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            4053..5987
                     /locus_tag="ACGE70_001242"
     CDS             4053..5987
                     /locus_tag="ACGE70_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFF8692890.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(6073..7296)
                     /locus_tag="ACGE70_001243"
     CDS             complement(6073..7296)
                     /locus_tag="ACGE70_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFF8692891.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(7290..9161)
                     /locus_tag="ACGE70_001244"
     CDS             complement(7290..9161)
                     /locus_tag="ACGE70_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFF8692892.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            complement(9182..10300)
                     /locus_tag="ACGE70_001245"
     CDS             complement(9182..10300)
                     /locus_tag="ACGE70_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFF8692893.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(10383..11216)
                     /locus_tag="ACGE70_001246"
     CDS             complement(10383..11216)
                     /locus_tag="ACGE70_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8692894.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(11209..12126)
                     /locus_tag="ACGE70_001247"
     CDS             complement(11209..12126)
                     /locus_tag="ACGE70_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8692895.1"
                     /translation="MIQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(12149..12328)
                     /locus_tag="ACGE70_001248"
     CDS             complement(12149..12328)
                     /locus_tag="ACGE70_001248"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692896.1"
                     /translation="MNKINENTSNTVLPATYSDATSYLTTAAYNYWQTGDFENSYLKE
                     AENFAHKYGVKLTGD"
     gene            complement(12353..13477)
                     /locus_tag="ACGE70_001249"
     CDS             complement(12353..13477)
                     /locus_tag="ACGE70_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFF8692897.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            13660..14568
                     /locus_tag="ACGE70_001250"
     CDS             13660..14568
                     /locus_tag="ACGE70_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFF8692898.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            14709..16322
                     /locus_tag="ACGE70_001251"
     CDS             14709..16322
                     /locus_tag="ACGE70_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFF8692899.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         5330 a         3285 c         2793 g         5464 t
ORIGIN      
        1 tattttattt ttataacctg gcatgctcat tagataaagg atttcatcgt aatggatata
       61 gtgatcgcta ttaccataac ggacagttgt ccaccttttt ttcgtagcat gttgaacagg
      121 atagttttct ttttctttca ataagtaact ttggatgctt tttttatttt tttcataagt
      181 gaattctttt atcaaataac cactaggcgt aatctttcga ttaataacag ttattgcttt
      241 attcggatga gacgttagat aaacaatttt acacttttca ttcttttctc taagcatttc
      301 gcctagttcg ataccgctat aataggcgtt caaatcgatg tctaggaaaa aaatatctga
      361 ggaatagatt tcattatttt taatgaattc aacaaattgt ataacgttca atccctgaac
      421 gatctcaaat ttccaaggac ttaataaaat tctggattct tccaaaattt ctactagcat
      481 tttttcaaaa accaaactat catccaaaat aaatatacgc gttaccataa aaagtacctc
      541 cactcgatat tttactatca aaatcatttc atacaacttt tcaacataca tgttaggaat
      601 aaattgtaat tctcttcaca atcttttcca acacctttga aaccgatatc aaatactttc
      661 aggatgctgc ttctaaaata aatatcaaac aaatgaatac atcatgcaaa aaaagccaga
      721 caaccgacgt ccttgcgtgt cagctgtctg agctatgttt atttattttg ttcttttaaa
      781 aagaacgata cacatactga tttttcggag cagagacttt ctcagcgttt gtaacaacta
      841 cagatgggat ttcacgcttg aatggttgat aatagtctga ttggatggtt ccttctattt
      901 tataccattc atcattttta accgttactc cttccggcat atgcgtcaac aaaccaaaga
      961 ctcctgaatc tgccacacaa tggataatgc cgaatcggaa caagaagata tctgtattgc
     1021 tatcgttcgg tgtttggtaa acaaatcctt catagctgat ttttttgcca atgaattcgc
     1081 tagggtaatt ataaattagc tccataactt ctaaatagtt ttcatctgtg atcttgatag
     1141 tttgcttatc cccatacttt tctttcaact cattcatttg gtctaagtag tctgatttgt
     1201 tgaaataaat actggtatcc ggctttaagt attgtgtctg catctccgga tcccctactg
     1261 attctttact tactgggaaa ttaaatcctt tggcttctac gatcgtagtg tcgagactaa
     1321 ctgttggaaa caacgtcccc acgattactg gcaaagctag cagtacataa gcgattccac
     1381 gttgataagg ttttgacaac ccatggtcat gatcgtgctc gtgtgccttt tcttctgctt
     1441 ttccgttttt gacccacaaa tacagctgaa ctaaagccaa aagagcagat aaaatcattg
     1501 aaagaatagc taagtagcga taatggacat tgatatattg atcgagtttg ccagaaactt
     1561 gcaagtacat catcaattcg aaatacccag ataaaatcaa aaaccgcagc atattttccc
     1621 tcctaaataa ataatgtgaa tccaataacg ctaagggcaa tcagtccgat cagcgataaa
     1681 ataaagcgtc ctgtaaaata tcgtttcatc atcagtagat ttttgatatc gaccattggg
     1741 ccaaagacca aaaaggcagc tacaggtgct gtgccgaaca gacttaacaa agaagatcct
     1801 ataaacgcat ctgcttctga gcaaagagac atcgtaaagg ccaataacag catgatgata
     1861 atggctaaaa tctttgttga gccgagcgtt agcataattc tagttggtaa ataagtctgc
     1921 atcgcagctg cgattaatcc gccaatgatc aaataacgac ctgtatcaaa aaattcgtcg
     1981 attgaatgtg tcaacacatg cccagtttga tggatcagct gattttttgg ttcattcttc
     2041 tcactttgtt cttcttcatg gacatgatga tggtgatttt ctgctacttt agacgtgatt
     2101 ttttctttca atacagcgtc tttttgaaaa taggcaagcc agacaccaat cacaagggca
     2161 acaaaaaagc tgcctatcac ccgccagctt gccatctgcc aagaattgcc aaatgcgata
     2221 aaagtggaaa aaatcacgat tggattgatg atcggagcag tcagcataaa agcaaacgct
     2281 gtgtgaactg gtacgccttt tttaacaaat tgatgaacga tcggtacgat gccgcattca
     2341 catgaaggaa agaaaaatcc taagatcgaa ccagtaaaaa tagagaagat acgattttca
     2401 ggaagccatc tcttcacacg ttctggggtc aaaaatactt gtaaggcgcc tgagataaga
     2461 cagcctaata atacgaaagg taatgcttca ataacgatcg ataagaaaat cgtactcatc
     2521 tgcaaaaccg aatttggaat aaaagaaaac atgagtttcc tcctttttat ttctagttca
     2581 ttcgctttgc aaatatacgc ttttattcaa aaaaaacaag ttgctctgta aatttttaca
     2641 caactcttat tttttaccaa agcacttcaa ttgacttttt ctgttattcc cattaaatta
     2701 aggtctcata catatcactt ctatcataaa agggagaata aaaatgaaac aaaccactaa
     2761 cttgactgaa gttcaagaca tactgcaaca gtcatccgtt gccgtcattt atctttcgat
     2821 gcctaattgc agtgtttgtc acgctgttcg tccacgacta gaggaacttc tcacacatta
     2881 tgatattccg gcacttcatt tggatgcatt tgaaacacca gaagtagcta gtaaatttga
     2941 agtgctgact gcgccagttg tcctgatttt tcatcaagga aaagaagtct ttcggcaagc
     3001 acggttcatc gactttaaaa aaatccgtta tctactagat gaactgacag cagaagatga
     3061 ttccctttct tatgaagaaa tcttccgaac aaaataatcg ttgtccaaac agaaaaaagc
     3121 gattgaacaa gtgaaagttt tgccttcatt tgttcaatcg ctttttattt ttattctgct
     3181 gtattttcgg aatgcgcatt tgcgtcagaa tgcaagaaat agatcaaggt ttgcaattca
     3241 ttcgtcaaat caacattttg gatcaataca tctgacggag cattcaatcg agcagcggta
     3301 aagttcaaga ttcctttgat tcctgcttcc gttaactgat tgactacttc ttgtgctctt
     3361 tcagcgggta tcgttaaaat tgctacttcg atttgttgga tcttgatttg ttctgacata
     3421 tcttccattg gataaacggg gataccgtct acgatgcgcc caacgatttc ctcattcaca
     3481 tcaaaagcac aactgatacg tatgctatta ctttgatgga atttgtattt tagtaaagca
     3541 cttcccaagt tccctacacc gatcagcgca acatttgtca attgatcgtc gtttaatgtc
     3601 tttgcaaaaa agtgcataag atcttctaca tcataaccat acccacgttt tcctaattcg
     3661 ccaaaataag aaaaatctcg acggatcgtt gcactatcta cttgaacggc ttcacttaat
     3721 tcagttgaag acactttttt ctttccagca ttatttaaaa tccttaaata gcgataatac
     3781 aaaggcagtc ttttagctgt cgcctttgga ataatatgat ctttcacgaa atgacctcca
     3841 cgtattgtct aattacatct ttgatttctc catcatagca gtatttatag gaaaaaatca
     3901 attccattgc tcagatattg tgaaatttta ttctttattc atttatttat catttctgca
     3961 aatctcgcag tataatggaa tttatggtag actactaatt gaaaaatagt tttttatttg
     4021 aggagaattc acaaagtaga aaagaggatt ccatgatatt attacaagcc aatcagattg
     4081 ctcgccattt tggtgcagaa gtcttatttg aaaacataca tttagaaatc agtacaggcg
     4141 ctcgtgtcgc tttagttggc agaaatggcg ctggaaaatc gactttatta aagatcattg
     4201 ctggaattga agcacctgat gaaggaaaga tcgctaaaaa taaaacagcg actctcggat
     4261 acctcgctca ggacacagga cttgattcca atgaaacagt ttggaacgag atgctgaaag
     4321 cttttgaaca agttagacaa atggaagagc ggatgcgcga gctagagatt ttcattagcg
     4381 aaggtacgcc agacacacct gcttatagtt cttatttaaa agaatacgac cggctgcaac
     4441 acgatttttc agatcgaaac ggctatggtt atgaaaatga aattcgttcc gtattgcatg
     4501 ggtttaaatt cgattcttca ttttatgatc agaaaatcga tactttatca ggcggacagc
     4561 gcacacgttt agctttagct cgcatgcttt tgcaacaccc tgacattttg attctagatg
     4621 aaccaacgaa ccatttagat atcgagacac tttcctggct tgagaattat ctgcaaagct
     4681 atactggtgc attattgatt gtttcccatg accgttattt tttagataaa gtagtcaatg
     4741 aagtctatga attaagcaga agaaaaatgc gccattacaa aggtaactat tcgaaatatc
     4801 tagatttgaa agcagaacag ttagcaagtg aatggaaggc ttatgaaaag cagcaaacag
     4861 aaatccataa attagaagac ttcgttgctc ggaaccttgt tcgcgcctct accacaaaac
     4921 gagcgcaaag ccgcagaaag acattagaaa aaatggaccg gctggatcgt cctcaagggg
     4981 atgaaaaaca agcaaacttt ctattcagta tagaaaagcc ttctggaaat gtagtcttgc
     5041 aaatcgaaga tgccgctatc ggatatgata atgtcacctt atctgaacca atcaatctag
     5101 atatccgtcg tcaagaagcg attgctctag taggtccgaa tggtatcggg aaatcgacgt
     5161 tgctgaaatc aatcattcac cagatccctt ttatccgtgg aaaagaaacg ctagggacaa
     5221 atgtcaccat cggctattac gatcaaggtc aagctgatct gcatacaaac aagacgatcc
     5281 tagcagaatt gtgggatgag catcctacca ctccagaaaa agagatccga agtgctttag
     5341 gtgctttctt attttctggc gaagatgtag aaaaaccaat ccctctgcta agtggcggtg
     5401 aaaaagcacg ggttgcttta gccaagttag cgatgaacaa agaaaatttt ttgattttgg
     5461 atgaaccaac aaaccattta gatattgaca acaaagaagt attagaaaat gcattgatcg
     5521 attatgacgg cacactgctt tttgtttccc atgaccgata tttcatcaat cggatcgcga
     5581 caaaagtcgt cgaactctct gaaaatggca gcaaagtcta tctaggagac tatgattatt
     5641 atttagagaa aaagcaggaa gaagaagaaa tcgccgaatt gttggcacaa gatgagccag
     5701 aatctgctcc tgctcccacg aacaaatctg attattacca aagcaaagaa cagcaaaagc
     5761 ttatccgtag ccttcaacga aaaattactc aggttgaaga agaaatggcg cggatcgatg
     5821 aatggatcga tcagttggaa caagaaatgg tcgcgccaga aaacctggaa gatcacgtaa
     5881 aactcaatga gctgaaccaa gatttagaag ctgctagaca agatcaagaa acaaaattga
     5941 cggaatggga agaactaagc ttggaattag aagaatatga aaattaacga aaaaaccaaa
     6001 acagaaacca ttcatgctgt tttggttttt ctttttttat tttatcaaac aaaaattcca
     6061 tatttacctt tttcaccaat aagaagacca attactagtc attctcacta aagcttctaa
     6121 ataatagtaa tcaccccaaa tacaaggtgt atttacccca ttatttttgg gcttattata
     6181 aacagactgt gttaaaaagc catcttcttc ttccgttgta gcatatcttt ctttcaattg
     6241 tcgcataatt ttccaaacag cagactggta taagttcttt tctgttttgt ctaatagacc
     6301 tgattctatt aattctaata atccacaaac catgatcacc atactagatg tatctttttc
     6361 ttctctgcta gaatcatcaa agatcaaatc ccaataacaa atggtatctt ctggcaaccg
     6421 attcataaaa taattggcta atattttgct taaatcaaaa tatttttgtt ctttcaaata
     6481 acgataactt aatgcaaaac cgtaaatccc ccatgcttgg cctctagccc aacaggaatt
     6541 atcactataa ccttgatgcg tacttccata tcttggtttt ccatctttga catccatata
     6601 aaatgtatga taagttgaac catcttctct tactaaataa tctgctgcat tttgaatatg
     6661 agcctgagca gcttttttat atttttgatt tccagtttct tcactcgccc aatacaataa
     6721 tggaagattc atgcaacaat ccatgatcat tctaccttgt tgtgaaggat cactaggatc
     6781 tccccaagct tgtataactc ctgccttctc ccaaaaacgc tgaagtaatg tttctgcagc
     6841 atcaattgaa gcttccttca tttttcgatt tcccgttatt ttataatctg ctacggttga
     6901 taaagaatac aaaaaaccaa gatcgtgatg attagtgata atttgacagt caatcctgtg
     6961 gataaaatct atatctgctt tttcagctgt ttgttgaaat aaaggatcac ccgtcttctc
     7021 aaataacaac caattaattc ctgtccaaaa tccttctgtc caaccaatat tgtctcttaa
     7081 tggataacgt ccttttatag gacactcgct tgggaattga tactgactgt tactgatacg
     7141 tttgattaac ctctccgtgt gtctgcgaca acgctcgatc tcattattcc attctttttc
     7201 cattaattct ttataacatt catttatcca tttttctgga tgtattatct cttcaaacat
     7261 cactttatca agcatttccc caccagcctt tagcataatt ttagtctttt tttattgttt
     7321 ttattttgga tagttagccc ggttgtccca tcaaaatcaa ttttgggacg attgttccat
     7381 acttctaaac tataatcccg atcttgatga gcaaccactc ctgttccgag ccaatgggtt
     7441 ccttttccaa atgtaccctc taatccagga ataaaagtgt tttcccatgt catcaagttt
     7501 gtattgggca aacccataat agctgtggat tttcgtttaa tagtatcgtc acttaaacag
     7561 ataatacctg aaaagccagc ttcactcacc ctatagtttt ccttgtcttc ttctatgatt
     7621 attccttctt tctcaggaga acattcaata gcaaaccctg tctcatatag aacatattct
     7681 gtatctaatt ctactttgtg tacgcgaatc tgccagcctt caaaaggaat aagccaagtt
     7741 ctgatttttg cctgtaaagt aggttcccat gtagaggcaa gtacctcttt cttacataca
     7801 atgtctcgta cattatttcg aacgatgaac tgtccatagg ttttgatgct ctcattctta
     7861 aaactggtcg tgatatatgt gtccgctcgc tgaatcccta gcattgaatc cattgccatt
     7921 tctgctttca attgattcgc tcgcggtatc gaaaaaccaa attgactaga ataagcaaat
     7981 tttaaatatt tttcattggt caatgtatgg aaatttggcc ctaattgtcc cgcattcaaa
     8041 aaaaatgtat gtccattatc atgttgtatg agcaaattgg cttctgaaag aagtttagtt
     8101 ccttcacgaa taggcatagg catttcttta gccgtccaat actcatggtc ttcaggtagc
     8161 gccaacaata aaaaaatctt attcagccat aatggagata acgtactatt atagggttca
     8221 gtcaatgtca attgagggta ggtataacca atattcagga tacctttttc atcaaatatt
     8281 tctttttgca tccaccatct tatattccga ttgactaacc ccttgatttc acctaattta
     8341 aaaggcaaaa gatcggccca caacatagct gaccaaaagc ttgaaactgc aaaacgataa
     8401 atcatgcttc gaccaaaagg cacatttgca ccatcttccg caaaaaaatg aatatagtct
     8461 ctagaaaaat caatagctcg gttcttatat atctggctaa tgggcttttc tggttctagt
     8521 acgctataaa tcaaaccata ataatgaaat gcaaaaggat tataataatc ttggcgacct
     8581 cttgaactgt ctcgatacca gccattttga agataacaat tttctatatt atcaaatgct
     8641 tctttcagtg ctgattcttt aactggataa ccaaattttt ggataattgt tccgacaatt
     8701 atcttaaaaa attgccaatt accatctgca cattcatatt caaaaatttg cgagaaccaa
     8761 tgaataatct ggcttttttc tcctgtatct aattttttcc ataataattg ttcatgatga
     8821 tataacgcta ataacattgc gggcatttct accatacgct ggtctctttc atgtatttct
     8881 ccccaataat cgggatggga aggatctgtt ccagctttta ttcctgatag aattaatttc
     8941 aaccatcgtt catctaattg gtcaattgcg ggccccgctc cccataaaac ccgagagaat
     9001 ccttccattc ctgcaatttt atttccatat cctacccctt gatgtatata atcaatttgt
     9061 gcgccacttg ctcgataaaa cggctggagt ggttgattta tctttttaaa taagtcaaag
     9121 taatcctgtc tggtccttaa tagtggtact ggatgtttca ttgtattttc tcctcttttt
     9181 actaccaaat agctaattcc gactgttcta atcgaagtaa gccttcaata aaaaaataat
     9241 cagaatagat cagtggcaca ttataagtca gttctctatg gtatgcttct gctccgcctc
     9301 caatgatccc atcttcatcc atattccaat ttgcatagtg cttttctgtc ttttggagta
     9361 tattgatagc agcttcatga tacatttttg atcgtacaga ggaaacatat cctgctaatt
     9421 ccagaagacc gcatgcagcg catactccag cactggtatc aatattcgaa tcttttacag
     9481 caaaatcaat gagaggaata taaccagttt tttcgatttt ctgaataaaa tttactgcta
     9541 tcttttctgc tatagctaga tagtacggat tttttgtgta acgataactt aacgcgaatc
     9601 catataaagc ccatgcattt cctctgctcc aacaagaatt ctcattgtat ccttgcccgc
     9661 caagtaaccg aatatatttc cctgattctg catcaaattc tgcgatatgc ccggttgatc
     9721 catctttcct cactagatac ttagctgttg tatctgcatg ctgccgagca attttagaga
     9781 acctcggatc accagtttcc ctcgaagccc aatacagtaa tggtaaattc atcatactat
     9841 caataatcat ccaaccaggt ttatggtcat tccatgctac taaatagttc cctgaaatat
     9901 tatagcgtcc agcaagtacc gtagctgcat gtaaccctag ttgtcgacta tgtttgtttt
     9961 gagactgttg ataatgtgca atagccgatg gtaagaataa aaaacctaca tcatgatgaa
    10021 cgtctgaaaa atggtacagg gcagattcta ataattgttc ttgttgtatt gctgcctctt
    10081 tgaacttttc tcgttttgtc ttgtgaaaga gctgccataa aataccactc caaaaaccat
    10141 tggtccacca gtcgattcct ctgtccttta ttacgtctgt atattttccg ttgtaagaaa
    10201 tatatggaat tttattcccc attctgtcta attccgcatc tatcttttca gaagctacat
    10261 tcaaaatttt tcttgaccat tccttcgtct gttcattcat ggatatcccg ccattctttt
    10321 aatttttatg aatgtggagt tgctcctaga acgaactaga tggttaacat tcgttatttt
    10381 ttttacttat ttgcagctcc aatgtcattc tccttaaatt tagactgcgc ccacacataa
    10441 atcaagacca ttgggatcac tgaagttgct aaaattagca taattgcgcc ataattaatt
    10501 ggctcacctg tactagaaac cgtcatattt ttgataatta agggaaccgt atatttatct
    10561 ttatctgaaa gcatgactaa tggaaataga tattgattcc aacttcccat aaagataagt
    10621 aaaccactag taataatcgt aggtgataaa atcggtacag cgattttcgt aaaaatcacc
    10681 cactcattgg caccatcgat tctggcagcc tccatcagtg ctgtaggaaa ttgactgcta
    10741 tattgtcgca tcataaacac tgaagttgta ctggcaatcg aagggatgat cacacctgca
    10801 taagtattcg ataatcctaa acgactcata atgtaaaata aaggaatcaa tgtggtaaaa
    10861 aaaggcacca tcctagatag cataactgta ttgaacaaca gtttattccc cataaaacga
    10921 tactttgcta atgcataacc cgccattgta gaactggctg tagctaaaac tgtactgatt
    10981 acagcaataa caatcgaatt ccaaataatc aagggataat tatatctcgc catgatggct
    11041 tgccagtttt ttaatagcat atccccaaac ttgaaattga ttccgccact taaaatttct
    11101 tgattacttt gcgttgaagc tacaatcata tataaatatg gcatcaaaaa tattaaggca
    11161 ccaaacacaa taatgacgta cgtaaaaatt ctgctttgta tcttttgttt agtcattttt
    11221 atcacctccg aatttaaatt gaatagccga taatataaca gatacaaaca ccatcgtcca
    11281 ggcaatagca gaggcttttc caaaatcaac catttcaaaa cttgttttat ataaattgac
    11341 tcctacgacc aaagctgaat tttctggtcc attggaagtc ccaaagaata tatttgggat
    11401 attgaaatta cccaagccat tgatagtggc taaaactgtg gccattaaaa tgattggctt
    11461 tagcatgggg atcgttaata aaaagaattg ccgaaatttc gatgctccat ctatttcagc
    11521 actttcgtat atctctttag aaatcccttg aagaccagct aaaaataaaa tagtgttata
    11581 tcctacacta agccaaatca tcgtgatgat cattgatatt cgtatccaaa aaggatttgt
    11641 taaccactgt acaccgttaa ttcctagcgc attcaaaaat ccattgaata acccagtcgg
    11701 attgaaaaaa gtcatgaata cggaagctac agccaccgtt gatgtaatgg ttggaatata
    11761 atagacagtt ctaaaaaatc cttttccttt taactgtgct gaattcaata aaacagcaaa
    11821 gtaaatagct aatagcatcg ttattggaat tgatcctgcc atgataatca aaacattcgt
    11881 gtaagattta ataaacaatg gatctcggaa gatagctaga taattgtcaa atccaacaaa
    11941 ttcaaatgag tagccaattt tcataaaact aaaagcaaac gagataatga tgggaatcaa
    12001 aatcgtcaca ataaagaaaa taacaaaagg tgtgaccaat ccataagcac tttttttatg
    12061 agacgtatct gttgcttttt tcttgctgtt ctttattgtc ttgcttggta acgaagattg
    12121 tatcatcgtt aagtcctcct aatatttttt aatcaccagt taattttacg ccgtacttat
    12181 gagcaaaatt ttctgcttct tttagatatg aattttcaaa atcacctgtt tgccaatagt
    12241 tatatgcagc ggtagtcaaa taagacgtag catctgaata agtggctggt aaaacagtat
    12301 ttgatgtatt ttcattgatt ttattcaata gttcatagta tttttgtcct ccttagtagt
    12361 caaacttttt ctcagctgct tctgtttgat aggccaatga gttagatgta gccacgccca
    12421 gctccagtgc gttggctaag cattcttcat ctgtcagcat aaatgtaaga aattgttgtg
    12481 ccacttgtgg atttttggca tctttaggga cataaaaact tgatcctcct gatactgcac
    12541 cttttcctgg attttctttt gaaaaaggaa tcagtggagc catgcgccat ttgtcagatg
    12601 catcaggaaa attcaaagac atattcgtag cataccagcc gccagcaaca aacattgcag
    12661 attcttgaaa tgctttctca gcatcttgtg caccgtagaa atgtacaata tctgagtcga
    12721 tcatttcttt gattaacttt gcagcatgtt tcgcttcttt agtattcaga cggatatttc
    12781 cttcttcatc aagcaaagat tgattctgct gggctagcat gttgataaac aattcttggt
    12841 ccccagattc tggcaaggca attccaaaac taccagtttt ttcttttaat gcttttgcca
    12901 cctggatata ttcttcccaa gaactgattt cttcaaaatc tacacctatt tcagcaaatt
    12961 tttttggttg ataataacaa agtgctgggg taaaatcact agcccaagga ataatcgttt
    13021 cattactcgt ttgttgacgc aaaatattca atttttgatc ataaaactga tcgccatatt
    13081 tgtcataaaa tccatattca tcagcactat aaaaagcatc ttgaaatttt tccagcacac
    13141 ctgctgctga agtgtctgcc atggagataa tatccggcat atcttcgtgt gttaccaatt
    13201 ttggggtgat catttgtact gttgctgttc ccgaagctac attcacaatt tttaagtcaa
    13261 tatttgctcc agttttgcgg ttatagattt ttgcagcatt ttcaacatag tttgtcagtt
    13321 tgccgtttgc gacatatact tctaatgtag tggctttttt tatcattgga tctgcgatgt
    13381 ttgctgttac ttcttcgtta gtttttccac atgcacttaa gctgaataat agggtaatgt
    13441 ttagcaaagc tatcaccaat ttgtatcttt ttttcatgtc tatcccttct ttcttttcaa
    13501 gttcttatat aaaacgcttt cacttatgta tattaacgga attagtaaca caaatattta
    13561 tataaaaaaa tattttactt gtcgttttgg cttttcattt ttgttataat agtcattaat
    13621 tataaataga aagcttttta tataaaaaga gaaaggaaag tggttctttt gttaacaaac
    13681 aaccaatcct ccaatcttgt caaaaaaaca ctaacctatt ccatagatac ccatcttcat
    13741 ttatctgctg ctccctattt tcgactacta ataaatgaaa gcgctaattt atatgctgaa
    13801 tttgcacata aatctataga actagataat ccttcccttt tattcattga taaacagcaa
    13861 gaagtccaaa tcttttctac tcaaaaggaa acacaaactt ttatcattat ccagttttat
    13921 tttgatgagc aattattagc acatattacg ggacaatctt tttctataga tcaagaaata
    13981 acatctatca ttacttttct gaaaaatgaa cagttcaaaa ttgattatct aaaacatagt
    14041 aagccagcta tttcctatga tcaggaaatc aattcttttt tcatactatg ccaacagatg
    14101 agcattcatt tgctttcctt attagttact tattgtctgc aaaaaattca attgtcttcc
    14161 ttaccaaggc attatcaaca ggaacttgct ttattgacaa taccttcaaa tgagaaagtc
    14221 attcctaaaa aaaagcatat tctttattca gatgaattag ttgatccaat catacgatat
    14281 ttagaagaac atctttcaga aaattttttg cttgaacaat tagcccaaat tttttttatc
    14341 agtccagcca gcttaaaaaa acattttaga aaagtgaatg gaaaaagtgt tattagctat
    14401 tttaaagaac taaaaatgga aaaagcaaaa cgtctcgtgc tagaaaatga attaacctat
    14461 acagaaattg cagatatatt aggatttcat tcgatccatc atttttcttc agcttttaaa
    14521 aaatatacag ggttatcccc ttcaaagtat tatctatcct taaaatagaa aaaaattttt
    14581 aggttttatc cacaaattat ttctctatat tttctgtcta ttcaaaataa acaataattt
    14641 tccggcacaa ttatttgctg ttttttacca taacatagta cgatcaacgt agaaaaggag
    14701 atgtttttat gaaagttgtt gtcattggcg gtgtagctgg cggtccttca tttgccactc
    14761 gtttccgtcg attgaatgaa gcgcacgaaa tcattatcta tgaacgcgga gaaaatattt
    14821 cttatgcaag ttgtgctttg ccttattatt taggaggtgt gatcactgac cgtgactcgc
    14881 tgatcgaacg tacaccagaa atattgaaaa caaaaaacaa catcgatgtc tttactaaac
    14941 acgaagtaac agcaatcgat ccttctacta agcgattaac agttaaagac ctatccacaa
    15001 atgaagaaat aaaaacagat tacgataagt tgatcatctc ttctggtgct agaccagatt
    15061 atccggatat tcccggagtt tttgaagcag aaaacggctt tgtcctccgt agtgtgacgg
    15121 atgcggatcg aatcaaatcg ttccttgaag aaaaaaatcc acaacatgtc gtcattcttg
    15181 gtgcaggtgt tatgggtctg gaattagctg agaatttcaa gcatcgcggc ttagacgtga
    15241 ctttaatcga tcaattgcca caagtcgctt tcccttatga tccagaaatt gctaatttag
    15301 tttatgacaa attgctgaaa gaaggattag ccgttcattt agaaacaaga gttactgaga
    15361 tccgtgataa aggtcgagaa atagtattat cagatggctc cgtcctttct gctgatatgc
    15421 taatttttgc tgttggtgtt tctccgaaga atgaagtggt gaaagcaacc ggcatacaat
    15481 tatcagatac aggtcagatc attgttgatg accagttaca aaccaatctg ccggacatct
    15541 atgcgattgg cgatattatc gaaacgacta gtgtagtgac tggtcagccg atccaaagta
    15601 tgctttccag tgcggccaat cgtcaaggac atatgctggc agatatttta aatggtacgc
    15661 ctatgcgcta tcgcggatat attggtgcag gtgtcgcaaa aatctttgat catacagcaa
    15721 gttatgcggg aatgacagaa catgaattaa aagcatcagg cataacaaat tataaaactg
    15781 tttttatcac tccctttgac catgcctatt tctatccaga agctacaaga ttaaatctaa
    15841 agctgatttt tgatgcagat agcggtcgta ttttaggtgg acaagcattt ggagaaaaag
    15901 gtgtcgataa acggatggga gaactttctg tagcaatcac cggaaacttg acagtctttg
    15961 atttgcctga tttggagttg ccttactctc caccgtattc tactacccgt gacccgttga
    16021 atatagctgg ttatgtcgca atcaatcaaa tgacgaatat cgtagaaacg atcaaagcaa
    16081 gtgatatacc cgaaaacgat ttgaaagaag cgttcttttt agatatacgt gaacctaata
    16141 aagcaccttc tggcagtatc tcggcaacaa aaaatattcc aatgaatgag ttacgagatc
    16201 gaatcaatga aatcccaaaa gataaaaaag tttacattac tttcagaaga ggattgaata
    16261 cttatacttc tgcccgaatc ttggcaggtt tgggtatcaa agcgattttg attgaagaat
    16321 aaaaaaacga ccgcggtggg tagccgcggt ctaggagtta tttttacttg aggagtaaaa
    16381 atgaaaaagt atatagggtt gttggttggt atgtatatag aatagaagat aaatgtgaag
    16441 aaattgtgca ttatttcttc acatttatta ggttatagtt taagaaagtt taaacattgc
    16501 gcaatttaat tattttagct gtttcttttc ataaaaaaac atgttgtgac tctgagacaa
    16561 atcatagtcc atagtgatgt ctctagagta actacgccta atattacgag gcatcatatt
    16621 aatctatcag cactagtacc taaactagca tccctttcga atattttttc ttatataata
    16681 aagtttgctt tcactcctct aatctcacca aatcgactgg cttgcgttgt gtgatactag
    16741 gaactgattg gtttagaacg acttttctat tgtaatcata tttttcgcca tcctcgttgt
    16801 cttctaatcc ttcaatgaat gatccctaat gtgtgtatta aataatttaa tttttttgtt
    16861 cctatttatt ta
//
LOCUS       DAXWOO010000047        16865 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.047, whole genome shotgun sequence.
ACCESSION   DAXWOO010000047 DAXWOO010000000
VERSION     DAXWOO010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16865
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.047"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            52..1053
                     /gene="ccpA"
                     /locus_tag="ACGE70_001252"
     CDS             52..1053
                     /gene="ccpA"
                     /locus_tag="ACGE70_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFF8692900.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(1390..3735)
                     /locus_tag="ACGE70_001253"
     CDS             complement(1390..3735)
                     /locus_tag="ACGE70_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFF8692901.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            4014..4916
                     /locus_tag="ACGE70_001254"
     CDS             4014..4916
                     /locus_tag="ACGE70_001254"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFF8692902.1"
                     /translation="MLLGSHVSMSGKKMLLGAAAEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            5129..6904
                     /locus_tag="ACGE70_001255"
     CDS             5129..6904
                     /locus_tag="ACGE70_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFF8692903.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(6970..8556)
                     /locus_tag="ACGE70_001256"
     CDS             complement(6970..8556)
                     /locus_tag="ACGE70_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFF8692904.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETVEEIE"
     gene            8807..9031
                     /locus_tag="ACGE70_001257"
     CDS             8807..9031
                     /locus_tag="ACGE70_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFF8692905.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            9172..10923
                     /locus_tag="ACGE70_001258"
     CDS             9172..10923
                     /locus_tag="ACGE70_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFF8692906.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSVKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTK
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIVMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPVKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            10923..12707
                     /locus_tag="ACGE70_001259"
     CDS             10923..12707
                     /locus_tag="ACGE70_001259"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8692907.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRTTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVLVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            13033..13884
                     /gene="licT"
                     /locus_tag="ACGE70_001260"
     CDS             13033..13884
                     /gene="licT"
                     /locus_tag="ACGE70_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFF8692908.1"
                     /translation="MLIEKVLNNNVVITRDERDREMIVMGKGLAFKKKVGDPILDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVHLNDSVYISLTDHLY
                     TAIERQKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GAKSEDGEVSEELFQVIQKKYKDTFGCVEKIADFLTGAFDYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            13980..15860
                     /locus_tag="ACGE70_001261"
     CDS             13980..15860
                     /locus_tag="ACGE70_001261"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFF8692909.1"
                     /translation="MGKYQELAENIVKNIGGKENIRSLTHCVTRLRFKLKEENKAKDD
                     VLKKMEGIVTIMKSGGQYQVVIGNHVPDVYAEVLTVAGISDEAEEEVSSSSNLFNRLI
                     DILSGCFQPFLGALAAAGMVKGLNALFLFLKFYTEGSGTYIMLNGIGDAIFYFMPVIL
                     GYTAAKKFHLNPMVGLVIGAALCYPTVQGAALQSGFPDGSPAPYSLLGLPAYETFLGI
                     PWVGSSYTSSVVPVIFIIAFAGQVQKIAKKWIPAVVQTFLVSFSVLLIALPAGFLIIG
                     PIVSILTDLLSTGFQALMDFSPAVYGAVLGFLWQVLVIFGLHWSIIPLAIMQLAQNGW
                     SQMLVGVYGASFAQTAVVLAMYFKFKDQKMKALCMPAVISGIFGVTEPAIYGITLPKK
                     KPFIYSMIGGAVSGIYLMASGTISYIMGGLGIFGVVNYITETDASGMFQAFIGIAIAS
                     VIGFSLTFFFWKDEAGETDAIQKKSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGRGI
                     LIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSETGLELLIHVGLDTVQLEGKYFESLVQ
                     QGERVRQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGETVGPDDALITA
                     LT"
     gene            complement(15900..16802)
                     /locus_tag="ACGE70_001262"
     CDS             complement(15900..16802)
                     /locus_tag="ACGE70_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFF8692910.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKYLLLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNDVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
BASE COUNT         5240 a         3051 c         3520 g         5054 t
ORIGIN      
        1 atgtaagacg tattttcaga aaacatataa aatatgaata ggagaacaca aatggaaaag
       61 caaacaatca ctatctatga tgtagcgaga gaggcaaatg tttcgatggc tactgtttca
      121 cgtgtcgtaa atggcaatcc aaacgttaaa ccagcaacaa gaaaaaaagt cttggaagtt
      181 atcgatcgct tggattatcg tccaaacgca gttgcgcgtg gcttggcaag taagaaaaca
      241 acaacagttg gtgtaatcat ccctgatgtc agcaatatgt tctttgcttc tttagcacgt
      301 ggtatcgatg atgtggcaac aatgtacaaa tacaatatta tcttagcaaa ttcagatggc
      361 aacgaccaaa aagaagtcaa tgtattgaat aacttactgg ctaagcaagt ggacggcgtg
      421 atcttcatgg gtcaccatat cacagacgaa atccgtggag aattttcacg ttcaaaaact
      481 cctgttgtat tagctggctc tattgatcca gatgaacaag taggtagcgt aaacattgat
      541 tatgtatccg caacaaaaga tgcagtaaac aaattggcaa aaagcggcaa taaaaaaatc
      601 gcttttgtca gtggtgcgtt gatcgatccc atcaatggac aaaatcgtct gaaaggctat
      661 aaagaagcat taagcgaaaa taacctacca tatagcgaag gattgatctt tgaagcacaa
      721 tacaacttca aagatggtct atctctagtc gatcggatcc ataatagcgg tgcaacagct
      781 gtctatgttt cagatgatga attagccatc ggtattttgg atggtttatt agaccgtggc
      841 gtgaaagtac cggaagaatt cgaaatcatc acaagtaaca attcactact gacagaagta
      901 gctcgtcctc gtttaacaag tatcacacaa ccattatacg atttaggggc ggtatctatg
      961 cgcctattga cgaaaatgat gaataaagaa gaagtcgaag aaaaaacgat tatcttgcct
     1021 tatggaatcg aagaaaaagg atctacaaaa taataaaagg acaaccattc agaggttgtg
     1081 tgcctgccgt ttaactctgc gttacaagga gcaatttcaa aaaacagcat ctcatgtttg
     1141 atgaatattg tgactcgtga ttcggagttg gcaggagaac accgtttatt tgggaaaagg
     1201 ggttatgaca aaattttgtt ataatccttt ttttgtgtct taaaaaagag agaaagtggt
     1261 tgaaattaca tttatcgtat ataaaacaat ccgtgtatag atgctgcatt ttgttcacca
     1321 ccatcaaaaa aataagggac tgtgacaata ctattgtcac aatcccttat tactgtgtat
     1381 tctattaaat caatcatctt tctcatctga ttctttagtt gcttgattcg ctcttgcata
     1441 agctgaagcg gtgttccagt aatctgtata gttttcatcg gtaccgccaa cgccaaattt
     1501 aaaggcgctt ttcttcggtc catttttcgc ccatagcgat tcaactgttt ttcctggtgt
     1561 ttggatggat tttttattga cagtaacttt tccaggtgtt tgaccagtaa atccagatac
     1621 ttttttcttc atcacatcag atgataattc gaatttctca tcagtgccaa aaatgtcagg
     1681 ttttgcttgg taagcttgat tgatcagatt ggcaagataa ttagccgaac gtatccttgc
     1741 tttgctgtcc attccagtat tgtcatcgtg ccctgcccag ctactgatgg taatagacgg
     1801 agtagagaca attagccatg aatctcggta ttcattggta gaaccagttt ttccgaccca
     1861 atctgctttt cctagatttc cgtttaagct tgagatggca tctttgaatg gtgtagtgat
     1921 ttttgcattg atcacactac gcatcatgtc attcatgatc gatgccgttg cttccgaata
     1981 gatccggaca gagttgcttt cgtgtttata aattacattt cctgcgttat ctgtgatgga
     2041 atcgatgata tagccttctt catagacgcc gccgtttgct aaagcctgaa atccgttggt
     2101 ctgttgaagc gtcgtgacat ctactgtacc taaaggagca gattcaacgc cccaattgtc
     2161 atttgccggg tagttcatct ttgccaagta gtgctcgtaa gcatattgtt tcgatccacc
     2221 ggaattcaac acatcttgat ataagtgata agccgggata ttgttggacc attctagtga
     2281 ctcacggacg gtttggaagg tgttcgatcc ttcgttagta gcattgacga ttttctcgcc
     2341 agcattttct ccttcttgcc aagttgttgc ataatctgat acacgggatt ctgatccgat
     2401 aagccccatg tcgattgcag gtccataaac aagaactggt ttgattgatg agccagcttg
     2461 acgttctgta tcgaaggcat ggttattttg attctgactg taattccgtc cgccaacgaa
     2521 tccgtagatg cggccagttc tgttatccat caatacattt ccggtttcta cttgactgcc
     2581 agcaccatca tctaacatgt atccataatt ttcaacgcca ttttgcaatg cagcatagac
     2641 atctttatcg attgtcgaat ggattgtata tcctttattt tggatcgttt gctgtgcctt
     2701 ttgataataa gcgtcactga ccgatgaatc agacatatca gcgttgtctt tttcagccaa
     2761 ttgttgtgca atgattcttg ttgcttcatt catcacagta taatacaaga attctctgtc
     2821 agtctgttct gcgatttgtt gtggtagaaa gtcttttgta aggtcatagg cttttgcttc
     2881 ttcgtattgt tctttcgtaa tttggccttc acgatacata ctgaataaaa caaaatcttt
     2941 tctggcaagg ccggcagaaa gatcttcttt tagcgcgcct gtgttcgtgt atggactata
     3001 cgtgatcggg ctttggggta atccagcgat ataagcagct tgcggcagcg tcacatcttt
     3061 tgcatctaca ccaaagattc cttttgcagc ttcctggact ccagcaatgt tttgtccctt
     3121 attattacga ccaaatggtg agacattcaa atacgtagca atgatctcat cttttgaaaa
     3181 gtatttttct acttgtgcag aaagaatgat ctcgtttgct ttacgtttga atgttgtttc
     3241 atctgtaagg atctgctgtt tgactaattg ctgtgtcaaa gttgatccac cagaagagcc
     3301 aataccagta gcttcagata ctaatgctcg taaaacagct ttaggaacgt aaccttggtg
     3361 tttatcgaag tattcatcct ctgtgctgat aattgcagtt ttcaataaag gtgaaatctt
     3421 atcagatgaa atagtcgtgc gcatcagatc tgtctggatt tttgatatct ctgtattatc
     3481 tgcataaaca atctttgacg tttctgtaat gtttcctagt tctgtctgca gtgctttttt
     3541 agtcggcaat tgtgtgtctt cgacgagata ggcaaaatag ccagcgccta ttccaatacc
     3601 tagagctgcg aacatcaaaa gaagaacagt aataaaaaca gtcaatgatt gaaaaactct
     3661 aaaaataatc ttaggaacaa accatttttc tttttttctt ctattctgtt tttttcgagt
     3721 atttttgcgt ggcaaaaggg tcacctcaac taatggtttt atttcgctaa ttatagcaaa
     3781 taatagaaca aaaaaacaga caaggctgtt tttggcagga atttcttttt taaaagagtt
     3841 aaaacgtgag aaaatgatct ttattataaa agagttctta agcttttctt tagagaaaat
     3901 ttcagttact atggagaaca gggcaaagaa aaatcaatca ttttctttgt atatcatctt
     3961 accaatatat aggaattgtt agtaacaatt gcctttcaag gaggaatcca tccatgttat
     4021 taggctcaca cgtcagcatg agcggaaaga aaatgctgtt aggagcagca gcagaagcag
     4081 ccagttatgg atcttcgact tttatgatct atacaggcgc ccctcaaaac actagaagaa
     4141 aagccatcga agagatgaat attgatgccg gaaaagcgta catggcagaa aacggtctaa
     4201 caaatatcgt cgttcatgcc ccttatatca ttaatttggg gaatacgatc aaaccagaaa
     4261 tgtttccttt tgccgtccaa tttctgagag aagagattgt tcgtgcggaa gctttaggcg
     4321 ctactcaaat tacacttcac ccaggtgctc atgtaggggc aggaacagaa gccggaattg
     4381 ctagaattat tgaaggcttg aatgaagttt tgacaaaaga acagacggca caaatcgctt
     4441 tggagacaat ggcaggtaaa ggtacggaaa tcggtcgaac atttgaggaa ttagcgcaaa
     4501 tcatcgatgg agtaaaatta aatgaaaaat tatccgttac ttttgacacc tgtcatacga
     4561 atgatgccgg ttataatatt aaagaagact tttcaggtgt aatggaagag tttgataaga
     4621 tcatcgggtt agatcggctt aaagtactcc atatcaatga ttctaaaaat ccgcaaggtt
     4681 cgcataaaga ccggcacgcc aatattggtt ttggaacaat cggattcgac acgctgaata
     4741 agatcgtcca tgatccaatg tttgacaata tttcgaaaat cttggaaact ccctatgtcg
     4801 gcaaagataa aaagaaagct tacgcaccat atggaaaaga aatcgccatg ttcaaagcac
     4861 aaatattcga tccagaagtg tttcctgagt tattagcaga agaagtgata ctctaagatt
     4921 ttctatctga cagcagcaaa aaaggaccag gcacattgcc ttgtcctttt ttgtttaaga
     4981 aacaaaggag tactttttgc agtctttttc atcatgcatc ttctcccccc tctttttaaa
     5041 tttagacaaa caaggtatac tgttagaaaa gtaaaaaaac gaaaagtttc acaagcaagt
     5101 ggcaaaacgt gattggaagg acgaaaagat gctgaacact ttaaaaccct tgatcaacca
     5161 actaaaaaag aaacatacat actttattct tggcatagcc ctattattta caattattcg
     5221 tttgtattta tatagtaaag caatctatgg aacagacatg tcggcaacct atgatgacca
     5281 gctgctgatt cgctatgctc aaaatatttt ggacggtaaa tggctagggg agtatacgac
     5341 tacaacactt tccaaaggaa tctcctacag cttattttta gtacttgcac ataaactgca
     5401 tctttcttat gctgttttgt tcggcatgtt cagtattttt gccagtatat tggttgcact
     5461 cgcgtttaaa ccgatcgtga agaacaagta tcttctttta agtatctatt tttatttttt
     5521 gttttctccg attagtttta cacatgaata ttccactcga acatatcgta atgccctcgt
     5581 gattccggct gttgagatca ttatcgcttg tttactagct atttattacc gcaaatacgg
     5641 ttctagcaga aaattgctgc catggttttt agggctttct gtcgcttttc ctttcttttg
     5701 gttcattcgc gaagattcta tctggcttct tccttttact gtagtagctt tgggtatcag
     5761 tttcgtccag atcttgctag ggcaagactt ttctttcaaa gaagtaaaga agttaaaaaa
     5821 acgactcttt cttctctcga aacgggaatg gctgaaactt tcattgtttt tacttcctat
     5881 agttggcttt tccatcgtct atcagtggat cgaatataaa aatgagacaa catacggtat
     5941 tgcaaccatc aatgaccgtt ccagtggaga atttggacgt ttgatgaaac aactgatccg
     6001 aatagatgat gaaacgaact tgaataaaga aaacagtgag atatgggtct cacatgctgc
     6061 actaaataaa gcattagccg catctcctac attccatact gtttctaccc gtattgacga
     6121 attatatacg actcacgcgt ggactcaagg tggaaaagta aaagaattac ccggagatat
     6181 catcttttgg gcattgcgag atgtggtaag cgaatctggt tattaccaaa acaatgcaaa
     6241 agaaaccaat gatttttgga aaaaagtcaa ccaagaatta gaagaagcct atcacaaggg
     6301 aaccatcaaa aagaaaaaag agttctatct gatgggatca ggagatggaa agacgatgag
     6361 tgatctgcca atcgtattcg atttccttca atcaggatac gcttatgcat ggcgatataa
     6421 agatttccat caaggaggaa attatagttt cggaacgcaa gaacaagtca aacaggctca
     6481 agaattatta aaccttccgc taatggagaa ttggatcgat agtaatgaac cgagaacacc
     6541 taatttccaa ttaacaaaat cagcaaaagt cgcgaatgtc atcataaaaa tttaccaagt
     6601 tctggctatt cccgtgctga ttgcttcaat agcagggata ttacttatga tactaggaac
     6661 cgtatttgca aaagctgatc gtgcctatta tagttcctct ttgattgtgg tcaccggatt
     6721 aatactgaca caattggttt ttcttttcgg tgtctcgtgg ttttgttctt atgcgccaga
     6781 aaaaaaggat tattttttaa gtgtctacac tggcgcaggc gtgccattaa cgcaattcat
     6841 cgtagtaaca agcatgctag ttttcagtaa attaaatctg aaaaaggctt tatcttctaa
     6901 ataaaaagac ccttggtagc tagattttta acctaactat caagggtctt tgtgtggaaa
     6961 aatcagtttc tattcgattt cttctaccgt ttcctgcaaa cgctgttcat attttttgtt
     7021 tcctttatat aaatcaccaa tcgtccatgc caacaggaga aggacaagaa caatgattac
     7081 ataagcgatg tgatcagcca atgctagttg agcagaagtg gcttgttcac caaagaaact
     7141 ttcgatctgt cctggtaatc ctcgtaagtt caagtaagtt agactaatca cagagaacca
     7201 gccaagaagt tttacgatga atgaattttt aaatcggcta cccatctccg cttggctgtt
     7261 ggtcatcatc agtaaaggga tcatcgaaaa cggcaacgca aaagccaaga acacctggga
     7321 ttcattcatc aagttattca aagcggtatg ttcttcgatc gttccttttg tacttgtaaa
     7381 caaaacacaa atcaatacag gaatcacaga aagcaatcgt gtgaccagtc ttcttagcca
     7441 aatcgggata cgcatatgga tgaagccttc catgatgact tgaccggtca acgtaccagt
     7501 aatagtagag ttctggccag aagcaagaag tgctacagca aataaagtgg ataagatacc
     7561 agattttgct acagcaatca agataccatt gctcatagct gatggatcag ataaggcttc
     7621 atacagacca aaaaaggaag gatcttggac agaaccggtt ttgaagacag cgacacccat
     7681 gatcaatagc agtgcattga ctagaaaagc catcgacaat tggatattag agtcccaagt
     7741 agaaaaacgt acagcttgtg ctacatcttc ttcgtcttgg tgatcaatct tccgagtttg
     7801 agaaactgct gaatgaagat ataagttatg cggcatgact gttgcaccaa taatccctaa
     7861 agctccagtc agcggagttt cgccgccgat gaccggtgag tctgagaatg tatgactgtt
     7921 agggataaag ccagcaaata aactgtgcca atctgggtta gacaagccaa cttgatagac
     7981 aaaaacgaat aaaatgacga aaattaagca gacaaccagt gcttcgatct tacgaaaccc
     8041 gacttttgtc agtagtaaga gaacgaaaac atctaataca gtaataaaga cggcaatcac
     8101 caatggaata tcaaaaagta agtagagggc gattgctgca ccaatgactt ctgcgatgtc
     8161 tgttgccata atggctaatt cagtcaagat ccataaaatg acacccaatg tcttacttgt
     8221 gcgtgctcgg attgcttggg ccaaatccat ttgggtcacg attcctaatt ttgctgccat
     8281 atactgtaaa agcatagcaa tcagactaga catcaaaata actgacatca ataaatattg
     8341 gaaattttgt ccaccagtga tcgaagtgga ccagttacct ggatccatat aaccaactgc
     8401 tactaatgca cctggtccag agtaagcaag aagtgtttta aaaaaacttt tcccctttgg
     8461 tacatcaact gttccattta cttcagctaa agataaacca tttgtatgtt caataagtcg
     8521 atgcttatga aattgtttgt tggattgatt attcaagaaa aaaccacctt tcctatttat
     8581 ctacgattac attctacacc tcaaaaagaa aaaattaaag aaaaatattc ggtatgccta
     8641 atttattttc gtggcgcgtc ttgttagatg acgaaagaaa ttttgttctc ctctaaaaaa
     8701 accactattt tttgcatgaa actaaagaat acgcctttct tttttcaata aactttagta
     8761 aaataagtca ttgagagcaa ttcggaaata gaaaggagag atttatatgt tatcaacagg
     8821 tattgtcgta cttattgcta ttattgcttt gctaatcggc gcagtaggtg gctttttcct
     8881 agcgcgtaaa tatatgaaag actatttaga aaaaaatcct cccgttaatg aggacatgtt
     8941 gcgatcaatg atgatgtcaa tgggacaaaa accatctgag aagaaaattc gccagatgat
     9001 gcagcaaatg aagaaccaag ggaaaaaata ggttctgacc caacgacttt ttacgggctg
     9061 attttttcag tccgtttttt attttgtaat ttgttagact attcagatta ttcagcaaaa
     9121 agacaagcac agacaatcct aggctcttga taaagacaaa ggaggagtaa aatgtcgatt
     9181 tttagaaagt taggctggtt ttttcgtcag gaaaagaaac attatttaat tggggttttc
     9241 tcgctattcg cagtggcctt ggtacagtta gttcctccaa aagtgattgg gatcatcatt
     9301 gatgagattg ccgataaaaa tttatcagta aagatcattt tcgggtggat cttcctattg
     9361 attatcgctg ctttcttaca atatcttttt cgttatatct ggcggacgaa tatttgggga
     9421 agtgcagctc ggttggagaa agaacttcgg cagcagttat tcaaacattt tacaaaaatg
     9481 gacaatctct tttaccaaaa atatcggact ggggatttga tggctcatgc aacgaatgat
     9541 ttgaatgcga tccagaatgt agcaggagct ggtatcttga cctttgcaga ttcagtgatt
     9601 acgggcggaa caacgatcat tgtgatgatt ttgtttattg attggcgctt aacactgatt
     9661 gcgctgattc ctctgccttt gctggccgtc acttcacgag tgctggggtc gaaactccat
     9721 gatgcttttc gtgattcgca agaagctttt tctagtatca atgataagac acaagaaagt
     9781 atcactggga tcaaagtcat taaaacattt ggacaagaaa aagaagatct agcggatttt
     9841 gaagaaaaga ttgaagatgc aattattaaa aacaaacgag tcaattttct agactcctta
     9901 tttgatccat tcattacctt gattatcggt ctgtcttatg ttgcaacaat tattttaggt
     9961 ggaaagtatg tcttggaagg cacgatctcg atcgggcaga tggtctcatt tattagttat
    10021 atcggtatgc ttgtttggcc gatgtttgcg attggtcgat tgttcaacgt attggaaaga
    10081 ggaaatgctt cttatgatcg cgtcaatgaa cttttgcatg aaaaaacgca tatcgtggaa
    10141 caactgggtg cagtgcagac cccggtaaaa ggcgagctga atgtagctat ttcctctttt
    10201 aattatccga aagaagaatc accagcatta gaacaaatca agtttgcaat ccaagaagga
    10261 gaaacattag gtattgttgg gaaaactggt gcgggaaaaa caacgatcat caaattactg
    10321 atgcgtgaat acgatgatta tcaagggaaa atccaatttg gcggacgttc gatcaaagaa
    10381 tattcattga atgctttatt aggatcaatc ggctatgtgc ctcaggatca cttcttgttt
    10441 tcgatgacca ttcgagataa tatccgtttt tctaatccaa aactttcaga agaagaagta
    10501 gagcatgcgg cttctttagc atttatcaac aatgaaatca aggaattacc aaaaggttat
    10561 gataccttag ttggagaacg tggagtctct ttatctggcg gacagaaaca aaggatttcg
    10621 attgctcgag cattgatcaa aaatcctgaa ttattaatat tagatgatgc actttctgca
    10681 gtagatgcca aaacagaaga agctatcttg cataatttaa aagaaataag aaaagagaaa
    10741 acaactatca ttgcagctca ccgtttaagt agcgtgatgc atgcaaaaga aatcattgtt
    10801 gtcgatcaag ggaaaatcat tgaacgagga acccatcatg agctgcttgc actaggcggc
    10861 tggtatgaac aaatgtggga gaaacagcaa ttagaagcga agatagaagg aggcgaggcc
    10921 tgatggatga aaattatcaa tcggaatgga caaaatcgat tccacttaaa gaacagaaaa
    10981 agatcatcaa acgactgatg aagttcgcca aaccttttag aacaacgttc atcgttgcta
    11041 tcttatttgc gtttgctctg tctgtgatca atgtcctatt gccaaggatc atccaaactt
    11101 ttatggatga tcatctggct aaacagtcgg ctacgacaca agttattttg ttttttgctg
    11161 gtatgtactt atttggagtc ctagtaaaaa gtatcatttg gtttttccaa tggtatttgt
    11221 attccatggc ttcattgaag acctatcagt atattagggt aaaattattt gaaaagctgc
    11281 atacactagg tatgcgttat tttgatcaga caccggcagg atcaaccgtt tctcgagtga
    11341 cgaatgacac agaaacgcta tttgaatttt ggtacgtctt tttgatggta ttgactggga
    11401 tctttgcagt cgtttcttca ttcattgcca tgtttcaaat caatggagaa attgccttgt
    11461 ggactttggt gttcttgccg attttaggaa ttgtcatctg gtattaccag aaatttagtt
    11521 ctcgtattta ccgaaatatg cgggaaaaat taagtcagct gaatacaaaa ttgaatgaat
    11581 cgatttccgg catgcgtatt atccagcagt ttcgccaaga agctcgttta gctaaagaat
    11641 ttgaacaagt gaacaatgag tatttagaca cgcgctatgc gatgatcaaa acgaattcga
    11701 ttctgttaag tccgatcatc aattttctat atgcattggc gattgcgttg gcgttgactt
    11761 tatttgggat cgatgcgttg cattctcccg tagaagttgg actgatctat gcatttacga
    11821 cttatgtcca gggatttttt aatccaatgt ctcaaatgat ggactttttg agcatcttca
    11881 cagatgggat ggttgctggt agtcgtatct tgaagattat ggatacggaa gaatatgcac
    11941 cacaacaaaa ccctggagca gatcaaatga ttacttcagg taagattgag tttcgcaatg
    12001 taagcttttc ttatgatgga gagcacaatg tattaaacaa tatctctttt gttgctaatc
    12061 cgggagaaac cgttgcatta gtgggacaca ccggaagtgg gaaaagctcg atcatcaatg
    12121 tgttgatgcg gttttacgaa ttttatgaag gtcagatatt gattgatgat catgacattc
    12181 gagaatatcc aatggaagag ttacgtaaga aaatggggct ggtcttgcaa gatgctttct
    12241 tgttttatgg ggatatcgca ggaaatattc ggatgatgaa tccagctatc acagacgaac
    12301 agatacgcca tgcggctgag tttgttcaag caaacaaatt catcgatgaa ttaccaaaag
    12361 gatatcactc aaaagtgatc gaacatggtg ccagctattc tagtggacaa cgacagctga
    12421 tcacttttgc gcgaacaatc gtgactgatc ctaaaattct agtattggat gaagcaacag
    12481 caaatatcga tactgaaaca gaaggattga tccaagaagg attggcgaaa atgcgacaag
    12541 gacggacaac gattgcgatt gcccatcggc tgtcaacgat tcgagatgca gaattgattt
    12601 tagttttaga caaaggtcat atagtagaac gcgggaatca tgaaacactt ctagcgcaaa
    12661 atggtttgta tgcagatatg tacaaactgc aaagtagtgg tatataaaag atgttgtgac
    12721 agtagcattt tgacctaagc actagagtga aaataaaaca tagcttccca tatttttcat
    12781 ctgtgagagt tagtgtcgta gttaccagat ttaaaaaaga cgacgtcagc tagtcagaag
    12841 tgcctaatag cccctaagaa agcgttgaca aaatcgtttg ctttcgataa tataaagaaa
    12901 acgatacaac ttggatagtg attgcctgat gcaagctaaa cctgatgagt ctcccatggt
    12961 ttcatggacg gctttttggg tttttctttt tgttttggat acagccctat atatcatgga
    13021 aaggagcaaa acatgctgat tgagaaagta ctgaataaca acgtcgtaat aacaagggat
    13081 gaaagagatc gggaaatgat tgtgatggga aaaggattgg cattcaaaaa gaaggtgggt
    13141 gacccgattc ttgactcatg catcgacaaa aaatttcagt tgacaaatga cggactttca
    13201 caacagttcc aagaattggc acaagcgatt cctcttgagt acttagaaat ttcttgtgaa
    13261 atcattgaat atacaaaaga ggctctgcaa gtccatttaa atgattctgt gtatatttca
    13321 ttgacggatc acttgtatac agctatcgaa cgtcaaaaaa tggggatctt tgtgccaaat
    13381 gtattgctgt atgatatcaa acaactgttt cctaaagagt atgccattgg gaaaaaaaca
    13441 gtgaagaaaa tctcggataa ataccacatc aagttatcag aagacgaagc cggatttatc
    13501 gcattgcatt tagtcaatgc acaaggcatt caaagccagc aagacaatac ctacctgttt
    13561 accacaacaa tacaggatat tttaaatatc gtccgctatt actataagat tacatttgat
    13621 gaagattcag tccacttttt tcgattcgtc acacacctgc gtttttttct gacacgtgtc
    13681 ctagatggag cgaaatcaga agatggagaa gtttctgagg aattgttcca agttattcaa
    13741 aaaaaatata aggatacgtt tggttgcgtt gagaaaatag cagacttttt aaccggggct
    13801 ttcgactacc agatgtccac ggatgaaaaa atgtatttaa cgatccatac tgcgcgttta
    13861 gtgcatgaga atcgttgtaa ataaataaaa aaggaggaca aattaacaag aacgtatgaa
    13921 ataagggtag tgacattcag ttggctaaac cttccactaa ttaattggag ggaatagaaa
    13981 tgggaaaata ccaggaatta gcagaaaaca tcgtaaagaa tatcggagga aaagaaaata
    14041 tccgtagctt gacacactgt gtgacgcgac ttcgtttcaa actaaaggag gaaaataaag
    14101 caaaggatga tgttttgaaa aagatggaag ggatagtcac catcatgaaa agcggcgggc
    14161 aatatcaagt tgtaatcggg aatcatgtgc cagatgtcta tgcagaagta ctgacagtag
    14221 ccgggatttc cgatgaagca gaagaagagg tttcttcaag tagtaatctg ttcaatcgat
    14281 taatcgacat attaagtggc tgtttccagc cttttttagg tgctttggct gcagcgggaa
    14341 tggtcaaagg attaaatgct ttgttcttgt ttctcaaatt ttatactgaa ggatcgggta
    14401 cgtacatcat gctgaatggg ataggggatg caatttttta tttcatgcca gtgatcttag
    14461 ggtacacagc agcgaaaaaa tttcatctga atccgatggt ggggctcgtg attggtgcag
    14521 cactttgtta tccgactgta caaggagcag cactacaaag tggtttccct gatggcagtc
    14581 ctgcgcctta tagcttgctg ggtcttccgg cttatgaaac atttttgggt atcccatggg
    14641 tgggcagcag ttatactagt agcgtcgtac ctgttatctt cattatcgca tttgccggac
    14701 aagtccaaaa aattgccaag aaatggattc ctgcagtcgt ccagaccttt ttggtttctt
    14761 tttctgtgtt attaatcgca cttcctgctg gctttttgat tatcggtccg attgtcagta
    14821 tactgacaga tctattaagc acgggattcc aagcattgat ggacttttct ccagctgtgt
    14881 atggtgcagt tctagggttt ctttggcaag tactggtgat tttcggactt cattggagta
    14941 ttatccctct tgcgattatg cagctagcgc aaaatgggtg gagtcagatg ttagtaggcg
    15001 tttatggcgc gagttttgca cagacagcag tggtattggc gatgtatttc aagttcaagg
    15061 atcaaaaaat gaaggcgctt tgcatgccag ctgtgatttc aggaatcttc ggcgtaacag
    15121 aaccggctat ttatggaatc accttaccga agaagaagcc atttatttac tcgatgattg
    15181 gtggtgctgt ttcaggaatc tatttgatgg catctggaac aatcagctac ataatgggcg
    15241 gattagggat ttttggggtg gtaaactaca tcacggaaac agacgccagt ggtatgtttc
    15301 aagcgtttat cggcattgct attgcttcgg taatcgggtt ttcgctgact tttttctttt
    15361 ggaaggatga agcaggtgaa acagatgcta tacagaaaaa atcaggtaaa gaaattgtaa
    15421 aaacaccgat acaaggacaa atcgctccac taaatgcagc gaaggatgca gcttttgcac
    15481 aaggaacact aggtcgagga atcttaatct accctgaaaa aggagaagta cgtgcccctt
    15541 ttgacggaac agttatgacc ctgttcccga cgaaacatgc aatcggaatg gtttctgaaa
    15601 cagggctgga attgctgatt catgtcggat tggatactgt gcagttggaa gggaaatatt
    15661 ttgaaagctt agtgcagcaa ggagaaagag tgagacaagg cgatctgttg ctacgcttcg
    15721 acaaaaagaa aatcgaaaag gaaggataca gtttagaaac acctgtgatc gtcaccaatt
    15781 acacggatta tttggatatt ttggaaaacc aaggggagac ggtcgggcca gacgatgcac
    15841 ttatcaccgc actgacctga acggttaggt gaagaatgga ctaggcttaa ctattctttt
    15901 tattccactt ctttattttc aaaattgaaa ggctctttca tgatggctga gctgataggg
    15961 cgtttatcga atttttgacc gacagaaatt agatggacta aattctgttg ataattttct
    16021 gcaaaaacac aactatataa cacgtctaga agataacaaa ttgatgtatt gatcgtaaaa
    16081 ttaccaattt ttgaatacag tcgctcgcgt gtcgtaagtc gcaaagcggc tgtactcatt
    16141 gcagatagcg ttgaatcacc gatactggtc aaagctaaaa ttggaacatc attttgtttc
    16201 aatatttctg cggtacgaag gatcacattg ttttcacccg tataagaaat cagaatcccg
    16261 cacatatcgc tcgtacaatt gtacgcttca tagaatgatt caccaaaagt ggtgacaatt
    16321 gtgacatcca ttttgatgcg cttcattttc agcatgaaat cttgagaaat caaagtattc
    16381 gagttactgg cgaagatcac gatttttttt gcctggagca gtaaatattt agctttttgc
    16441 agttggtcat gatgaatgag agaaagtgta tcatgaatcg tcatctgttc taaagaagcc
    16501 attttatttg cgattgtcat aataccgtct gtggtggaaa aaggaagatt cgcatctacg
    16561 tcttgaaaat tcccttcaag ataggctgtt tcagctaaaa aagcattacg aaaactgacc
    16621 cagccatcaa aacccatttt ttttgctaca cggatgagtg tagacgggtg gacataacat
    16681 cgctcagcta tcgcttggat ggtcatttcg ccaagtgctg gagaatcgct taagagaaac
    16741 tggatcactt cattttctga aggagaaaag gagatttctt tgattttttc acgtaataac
    16801 atgtagatac cttcttctta tttttttacg taaattataa cttttttgaa aatgttttgt
    16861 aaacg
//
LOCUS       DAXWOO010000048        16857 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.048, whole genome shotgun sequence.
ACCESSION   DAXWOO010000048 DAXWOO010000000
VERSION     DAXWOO010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.048"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(<1..457)
                     /locus_tag="ACGE70_001263"
     CDS             complement(<1..457)
                     /locus_tag="ACGE70_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFF8692911.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEK"
     gene            complement(562..1425)
                     /locus_tag="ACGE70_001264"
     CDS             complement(562..1425)
                     /locus_tag="ACGE70_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFF8692912.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(1621..3720)
                     /locus_tag="ACGE70_001265"
     CDS             complement(1621..3720)
                     /locus_tag="ACGE70_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFF8692913.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(3940..5049)
                     /gene="rpoD"
                     /locus_tag="ACGE70_001266"
     CDS             complement(3940..5049)
                     /gene="rpoD"
                     /locus_tag="ACGE70_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFF8692914.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(5073..6914)
                     /gene="dnaG"
                     /locus_tag="ACGE70_001267"
     CDS             complement(5073..6914)
                     /gene="dnaG"
                     /locus_tag="ACGE70_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFF8692915.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(7096..9411)
                     /locus_tag="ACGE70_001268"
     CDS             complement(7096..9411)
                     /locus_tag="ACGE70_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFF8692916.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(9596..10456)
                     /locus_tag="ACGE70_001269"
     CDS             complement(9596..10456)
                     /locus_tag="ACGE70_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFF8692917.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(10515..11147)
                     /locus_tag="ACGE70_001270"
                     /pseudo
     CDS             complement(10515..11147)
                     /locus_tag="ACGE70_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            11314..12075
                     /locus_tag="ACGE70_001271"
     CDS             11314..12075
                     /locus_tag="ACGE70_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFF8692918.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            12101..14692
                     /gene="mprF"
                     /locus_tag="ACGE70_001272"
     CDS             12101..14692
                     /gene="mprF"
                     /locus_tag="ACGE70_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFF8692919.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(14880..16067)
                     /gene="tuf"
                     /locus_tag="ACGE70_001273"
     CDS             complement(14880..16067)
                     /gene="tuf"
                     /locus_tag="ACGE70_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFF8692920.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            16435..16791
                     /locus_tag="ACGE70_001274"
     CDS             16435..16791
                     /locus_tag="ACGE70_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFF8692921.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         4631 a         3496 c         2956 g         5774 t
ORIGIN      
        1 ttttttcttt ttcatagcat tcctgacaga ttccatggaa cgttagccga tgatctttta
       61 ctaaaaaatg ataacgttct tctactactt gctccacttc aagtaaaaga tcctcttcta
      121 cttcctcgat cgtgccgcag ttcaagcata gtaaatgatg atggaaatgt ttggcgcctt
      181 cttttctcaa atcataacgg gctaatccat cattaaaact tactttatcg acaattttca
      241 attctgtcaa gatttcaagg gttcgataaa cagtagcaag tccaatttcc ggactctttt
      301 ttttcactaa aaagaagatt tcttctgcag ataaatgctc tttctcattt tccaaaagca
      361 caagcagagt agcttctctt tgcggtgtca gcttaaaccc cgcttcatgt agctgctgtt
      421 ttgttttttt catcgccgaa atagcattca tgaccattta ctcaacccca acaatttata
      481 ataattacaa ataaaaaaag atataacact atgttaatta aaattattat aagctagtta
      541 agaaaaacat gcaagggatt ctcaattaga ttttttgact agttttgtca caccgttttc
      601 ttgtgtaatc aatccttgtt tcaaaagatt acctaatgcg cgcttaaatg ctcctttgct
      661 gataccaaaa gctttcatga tttcttctgg atccgattta tctgtaaagt tgatttgctg
      721 atctgcagaa cgttctaaaa tggttaaaat catttgtgca tcatctgata tagcttcata
      781 tgctcttggc ttcaatgaaa gattcagtac accgtctggt cggacaccaa tcactcgacc
      841 ttttatctgc tcgcctagac gaggttcatt atatcgttca gaaggatgga taaacccaag
      901 atagaaatct tccgttaaaa cgtacgtacc tgttaatttc aagcggtatg ctgttcctat
      961 cacatctttg tttttcattt cttctgttcc atatttgctt aaagatttga atattttttc
     1021 atctgctaaa ctgccccaaa tacggtcttt tgcatcaact ctaagggaaa tcatcaagcg
     1081 atcccctttt ttaggccata gctctgtcat tgtcggcaat tcatcaagag atacgacaaa
     1141 gtctttatct ggaagaccga tatcgacaaa cgcacctaaa tcttttcgtg tagcagtaac
     1201 gattccaaaa gcgtaatgac cgatacggct tttaggaatt tgtgtagtga tccgattttc
     1261 atgtttttga ttttggtaac cgaatccttc aactgcttca ccgattttgt gttcaccttc
     1321 tgatttgctt aagccaaagg tttgtccgtt tttttggaca aagtagtttt tttcattttc
     1381 atcaataata atacctgtaa atatagttgc taataaatcg ttcatttttt tctccctcta
     1441 tagtgaaagg ttttcttaat acatttcttt gctatcataa cataatgtta tctacttgaa
     1501 tagctgagcc ttggtttgga aaggtgaaaa aaggagtatg acaacagttg tgtcatactc
     1561 ccctttttcg aataaacggt gttaaacggc aggctcacag cctctttcat ttgcctaaaa
     1621 ttaattgctc ccatttcctc cgaaccatcc agttttacag ttgatggtta cgatgtaaaa
     1681 tggcaaagca ctttctccaa tgcctttcat caatgcagat tttgttgaag tggcatatag
     1741 attataatag ccttccccat tttcgattct ggctttttct aatctgtaac ctccttcaac
     1801 gatatatcca cgttggatac atgtagcaat cactttgctt tggacacctg gtgcccacgt
     1861 ccatgctgga ttagatgcat cggcaatatc acttgctctg gcagatttga ttggctgatt
     1921 cgctgagttt gaagaggaag gggtcgagga tgccgcttct tgctgtgctg cttgtgcgac
     1981 cgcagcttgt tgagcggctt gagctgcagc tgctgcttga gcggcttgtt ttgcttgttc
     2041 ttcagctgct tttttcgctt cttctgcttt tttcttttcc tcttcttctt ttttctcttt
     2101 atcagtgagc atcttatcta cttgtttcaa ttgttcattc aactgagcag atagatctgt
     2161 atttttcact ttttctactg cttcttttgc cgtcttgtat tggtcgcgtg ttgctgaatc
     2221 cactactttt ccgtctttaa agactatagc tgttttttct ttagctgttt gcaattcagc
     2281 atattgattt ttagcttctg tttgtgcttg ttgaatcaat tttccaaaat ctgtgttatc
     2341 cgtttcgatt gcttggatct ctttatctgc tttcaacttg atgtcatctg ctagatgatc
     2401 atcgacgatg gcaggtgcta tgaaccactc attcactttt tggatatctc ggatcttaga
     2461 ttggatatct tggtaagttg tttctaactc tgaatatcct ttttcattct taagactgga
     2521 taaatcattt tttagttttg ataaatcttg atgaatcata tctgcacgaa taaattgatg
     2581 gtgatcatct gtataaaagg cagcaagttt ctcatgaagt tcttccattt gttgctgtct
     2641 ttttgcttct gctgctactt cggcttctac tttttgatga tggtcataat aagcccagct
     2701 tcctccaccg agtagaagta ctgctgctaa aacgagataa ggtacttttt ttgatttttt
     2761 cttttgcgct ttatctggat ctgttttttc taagtttgat tcttgatgtg ttttttcagt
     2821 atctggcacg attgttattg atttcgcttc tgcttcaaac agcttaggct ggttctcttc
     2881 tttttctatt tttgcttcaa gatcagtacc ctctttgctt acagattgat tttcagcatc
     2941 ggattcacta tttatttcat cgggctctgt tgactttttg tctgtgtttt cgagtatttc
     3001 ttttttctct gcttccggca gtttattcgt tttttccttt tctaattgtt tttcagtaaa
     3061 tacttcagtt ttttctgtga tatccgtttt tacgtctttt atttttactg tttctttacc
     3121 tgtggcgttt tctctatcag agtctttttc agtttcttgt tccttttctg gctctacttt
     3181 gttttcctgt gcaatttttt cactgatcat tttgagcagc tctttcgcgt gctcttcttt
     3241 tgtatcttca cgatgtgctt tgatataagc ggatagtatc gaattttctt cctcattatc
     3301 gacgatttct ttcagttcag ctactcttct tttttgtttt gattcaagcg tttcttcttg
     3361 attctttcct gattcttccg aagtctcatg aacagttgct ttcttctgtt catttttctc
     3421 tatattagtt acttgatcgt gagcaggtgg ttcttcgtct tctggttctg ctttttcgaa
     3481 taattccatt acagattcta gaggtaaatc tttcaattcc gaccattcga ttggatcgtt
     3541 taattctgta tcaggaaaga ctgcatcttc agttgattta tcctgcatag ctgcatctgt
     3601 atttgtttct tcaattgatt cttttatttg ctgatcttct gcagttattt gatgaccgca
     3661 gtctggacag atagtattct gctccatttt tttcgatccg caatttggac attttttcat
     3721 cacttagcga tcggctcctt tactaggttt acgctaccat ttaatctatc atattctata
     3781 cgtcacgcca atagcttatt tttaggattt acttaaaaat aatgaatgaa tttccttcct
     3841 atagaaaaaa aagcttgaga ctgcgtagtc tcaagctttt tttcttttaa aataaaccaa
     3901 acagttttac tgatttctct tctggtcaat agtccacgct tattctaaga aatcttttaa
     3961 ttgttttgaa cgagaaggat ggcgcaattt tcttaatgct ttagcctcga tctgacgaat
     4021 acgttcccga gtaacaccaa atactttccc tacttcttcc aatgttctcg tccggccatc
     4081 atctaaacca aaacgtaagc gaaggacatt ctcttcccga tcagtcaatg tatctagtac
     4141 atcttccaat tgttctttta gtaactcata agctgcgtgt tctgctggac ttgttgcttc
     4201 ctggtcttct atgaaatcac ctaagtgaga gtcatcttct tcaccgattg gcgtttcaag
     4261 ggaaacaggt tcttgcgcga ttttcaagat ttctcggact ttttctgttg gaagatccat
     4321 ttccgcaccg atttcttctg gcgtaggttc tcttccaagg tcttgcagca attgacgttg
     4381 gatacgtatc aatttattaa ttgtttcaac catatgaact ggaatacgga tcgtacgtgc
     4441 ttggtcagcg attgctcgag tgatggcttg acgaatccac catgtagcat aggttgaaaa
     4501 cttaaatcct ttgcggtagt caaatttttc aactgccttc atcagtccca tgtttccttc
     4561 ttggatcaag tcaaggaact gcatgcctcg tcctacataa cgtttagcga tacttactac
     4621 taaacgaagg tttgcttctg ccagacgctg tttcgcttct tggtcgcctt cttcgatttt
     4681 caatgctaac gctacttctt cttccgcagt taaaagggag acacggccaa tttctttcag
     4741 atacatacga accgggtcat tgatttttac accggttggc gcagaaagat cttctgtttt
     4801 tgcttgttca gcttttttct cagcactctt caagctgtga attgatggat caccattttc
     4861 atccaccaca ctgatacctg cgtcttcaat cttttggatc aatttgtcca tttcatctgc
     4921 attcaaagtg aatggtgtag caagctgatt ggataaatca tcatatacaa cttgtcgttt
     4981 gggtttgttt tctttgatga aagctgctac tgctgcttca tacgtttttt tatctgttcc
     5041 atttgccata aaagaaggcc ccccttgagt agctatttgg cttgttttaa ctgtttcgtt
     5101 aaattgatga tttcaatcgt caattcaagc tcaagctgtt tgtttccagt tcgtctggct
     5161 tcttgctgca tttgtttttt taatgttatt tcatcagcta agccagattt gctgatcaca
     5221 tctagcaagt cttggatttc tcgtggcgaa ctttcttctg ccaccggtaa atattcgatt
     5281 tccaccacca accgccttaa attttcatct tgcaaaaagt ctaaaaactc tgcaaggttg
     5341 aattcataat gttgtgagac atatgcatcg aataaaaaat aaacttctgc atatgcatca
     5401 tgaataaact gaaattctcg ttcttttaaa atattacgga cagaaacttc tttaaatgca
     5461 cgatataata gaatctgctc tgccttttct acttgcgtca aaggtctctt tttccgtgca
     5521 gtttcttgtc tcgtaacctg agtgtgttga ctttcctgag gttcttgacg attttgctga
     5581 tttgtccttc gaagatcgcg cagttgtttt tgtatcgtct ctctgccgat ctgaaactct
     5641 gttgacagtt gagatagata catatcttgt tcgattggtg aaacaactcg gctaagttca
     5701 actagtaatt cttcgaggta ttcgatcttg tctttttcat tatctaaatt tttcccttgt
     5761 tttaaataac gtgatttaaa agtaaagact gtttcgcgcc catgagccac taattcttgg
     5821 aaagatgctt ctccatactt acggagatat tcatcaggat caagcttttc tggaacgact
     5881 acgacagaaa gtgctaatcg actgttttgc cgaagcaatt caatccctcg attggttgct
     5941 tcaaaacctg cgttgtctcc gtcatagcaa aagaccaatt ctttcgtcag cttttccatt
     6001 tgttggatct gttgagctgt tagacttgtc cccatagaag ctaatccagt tttgatacca
     6061 gcctgccatg cagcaatcac atccataaac ccttcgaaca acaaaatttc tccgtttttc
     6121 cggatatcgc ttcttgcttt atcaagatta aataaaactt gtcttttatt gaacagttct
     6181 gtttctggac tattcaagta tttgggctga tctttttcct gccctttttc aggtgcaagc
     6241 cagcgtcctg aaaaaccgat cgtctggcct tgagaattac gaagtggaaa catcactctt
     6301 tgataaaaac gatcagacat acggccatca tcatgcgtga caaacaatcc agagcgttct
     6361 aacaattcat ttgaaattga ttcattcttg aatacttgct gaagaaaaga acgctcatta
     6421 ggtgcaaaac ctatgttgaa ttcttcaatt aattctaaag acaacccacg actttgcaaa
     6481 tattctaaag ctggctgtcc agcttttgta tgcaatagca tgtgatgata gatttccgct
     6541 gctttttcat gagcagcaat cagtttcccc gtagcagatt caggattttg gacagctata
     6601 gtacgctgac gccattgatc ttctaacggt atctgttcga agtctgccac ttttaccact
     6661 gcttctggaa aacttagacc ttctagttct tgaagaaaag taaacacgtt tccgccttta
     6721 ccacaaccaa agcaatgaaa aatctgcttg tcttctgcaa cagaaaaaga cggtgtacgt
     6781 tcttcatgaa aaggacacag ccccaagtaa tttttaccag actttttcaa ctggacatat
     6841 tgtccaatca cctcaacgat attcgtttga ctgcgtactg tttcgataac ctcttgagga
     6901 atccgttgag ccattttttc accccctgct ctattgatat cttttgggtt taacagaaga
     6961 tatttcagga gaataaccgg taaaaaatcg cactaactga aatcagtgcg attttaaagg
     7021 acacaattat acatgtttga ttgtatcata tttcattgct ttttcaagta taacacattt
     7081 ctatctcatt aagcatcaat ttgctactct tttcagtaac tgattttgaa tatcagcctt
     7141 ccagtacaat actcgaagaa caatatttac gagcatgaat acaaagaagt atcctacgct
     7201 ctctcccaac cctaagttta cttccaaaat tccatggaac agcattgcag ctacatatat
     7261 ccctacgatc gtcgttactg ctaggaagat gcgcaatggg ttgaagggat aacaagcttt
     7321 tacaactgcc atgcaactga tcccaatcag caagtaatac atcaaagtag tagtttcagc
     7381 atttccccat gcgaatgttt ttccaagtag atacaccgca atgatattca ctactacaag
     7441 caaggcattt ggtaaagcat taataagtgc tgtaggcaaa taacggtaag tgatttttct
     7501 tttatcttgt tcaaaagata aaaagaagga aggataccct tcaatcgcta aatcaatcag
     7561 cgtgatttgg atagggataa acggaaatcc aattgctgta acggcacata taatcgataa
     7621 aataaatgaa taaatcgttt tgataaaaaa gatgcctgag actttcgtca cattgttgac
     7681 cactcggcgt ccttcaaaca ggacttctgg caatgtagta aaatcagagt ccaacaatac
     7741 cagattcgca atctgacgtg ttgctccatc accttctgcc atagcaatgc tgcaatctgc
     7801 ttctcttaac gccaaaacat cattcacacc gtcgcctgtc attgcaaccg ttctgccgct
     7861 ttctttcaat tcatttacca gcaatttctt ttgctgcgga ctcactcgtc caaaaaccgt
     7921 atagcgatga gcagcttctc gcacttccat ttcttcagta atttgagaaa gatcgacata
     7981 cgcatcataa ttcggtaatc ctgcacgtct agcgatattg gagacagtaa aaggattgtc
     8041 tccagaaatc actttcagat ccacaccttc ctcatgaaga taagctagtg tttcttgagc
     8101 gttttttcgg attggatcat cgatttcaaa cagagcaacc ggctcaacaa atgctggttt
     8161 ttcttcgttc aattgctgat ttggagcgat ccccaacata agtacgcgat agccattcgc
     8221 ttgggctgtt tttagactgt ccggaagccg atcttcttcc accaaacgtt caggcgctcc
     8281 tacgtacacg actcccagtt ctggaaattc catcgcgccc catttgcgat cagaagaaaa
     8341 tgcgactttg tttgtcacat cataccgatt agaaagctca aaataatctc tcatagcccg
     8401 catagtgatg ttgttatcac tgctttctgc cagatacgtc cccataattt ctgggatggc
     8461 tttctcgtac agcggatgta gtgcttctac ccgctggata ctcattttcc cttcagtaat
     8521 ggtccctgtt ttatccaagc ataatgtatc gacatgggcc agcgtttcaa ttgaatacat
     8581 gtcttgaaca aggattcgtt tttttgccag tttggttacc cctgtcgtta atgcaatact
     8641 tattagcaat accaaaccct ttggaagcat gcctaacaaa gctgctgcag atgcaataac
     8701 agaaattttg atagaagcat cgcggatgaa cagtgcttcc aaaaatagta tgatccccaa
     8761 aggaataatc acgaaactag tgaatttgga tactttgcga atcgaatcta caagttcaga
     8821 acgaatagct ttgtgagtct ttgcctcgct agtcaagcgg acagcataat tatctgcacc
     8881 tactcggata acttcagcca tgacagaacc gcttgtcaaa aagcttccag aaagcaattc
     8941 agctccagtt tctttgacaa tcaaatcaga ttcgcctgtc aaaagagctt catttgcttc
     9001 tgcccgtcca gatataacct gtaaatcggc tggtatctgt tctcctgctc cgatttttac
     9061 caaatctcct aataccagct ctttagtaga aatcgattgt tctttaccgt cccggatcac
     9121 tagtacttct gattgggaaa caatcgataa tttccgtaca agatttcttg catgtatttc
     9181 ttgataaatt ccaataacga tattgacgac aataattgct aaatacgcca tgttggaata
     9241 cgcacctaca aataacagac aaatcccaat accaaagttc agaaaattga ataacgtcaa
     9301 tatattatcg ctgaatattt gttttgtact ttttgatgca tcttcctggt agtcattttg
     9361 ctggccagaa gccattttct gaactacctc ttgctgtgta agtccattca ttttcttttt
     9421 ctccattttc cgccaacttt caaaaataat cacgtgttta agtctagcgt gttccaagat
     9481 taaaaacaac ttgtttttat aaaatttata gaaaaacaca cgaatgattt tacaaaaaag
     9541 atgccaacct ttttgtttga atcacccgtg tgctgttttt attatcaatc tatctttact
     9601 tatattgttc ctccaaacgg ctcatccacc aacctagtgc tgtaccggct ataaaaagaa
     9661 caggacaagc tagtacccca gcaactccca atcccgcgta atcggtagga atgatcataa
     9721 tcgttgaagc aaaaactaca cctaaaatga aatggaatag cttgctatat gcctttgaga
     9781 aaatgtaatc catgattttt gacaatccaa gaacacaaag aatcccacca attccgatcg
     9841 gcacaatcac ttcaagtcgt acttctttaa atccgtcagc catcgcttta tacaatccca
     9901 tatacaccaa aaaattagaa gggctcaatc ctgggacgat catacccaaa ccaatcaatc
     9961 cgccggcaat cagccatgtt ccaaaattct gatcgacttg cccgtcaaat aattgagcgc
    10021 cgtataataa aaacaaataa gccagaacaa aagtaacaac catgatgatc acatcagaag
    10081 tatctcttcc ttttttccct gcttctttcc ataacgcagg aacagttcca gcgatacatc
    10141 cgacaaaaaa ccataatatc gttgtttcat acgcacctaa taaaaaactg actaaaaaag
    10201 aaagtagaaa gatgcctgtc actccgccta gaccaacagg cagaaaaaac aaaacatttt
    10261 ctttgaaatt ttttgtaagg tgggctaaaa aagaaatcag ccgctcatac atgccaaaga
    10321 cagctgctaa tgcaccaccg ctgactcctg gcaagataaa tcctgaaccg ataaacattc
    10381 ccttcacaaa ccgtaacatc cagtctttgg ttgtgtaccc cacttcttga ttttccattt
    10441 tttgcctttt ttccactatt tgcgcactcc ttctttcatt taattttatt catgattgaa
    10501 catttcctgc tattttactt aaagactttt tcataggtct ctattatttt ttcattcatt
    10561 ttgacaatat caggataaaa catgttacct ccattatgat agagatagcc gccattgtaa
    10621 agcaaggact ggatccgcca atatcggtat tgtttccctt cctcatttcc taaaagtggg
    10681 ctcagcacat ctttagaata cgcttctgct aattctactg tgttttctcc gccatgattt
    10741 tttacatagg caatataatc tttcccaaaa ttatatgctt ggacagctgt ccataaatcg
    10801 catccttgtt tctttgcttc tactatcatt tccgccaaat atttgacacc ttgatcaata
    10861 ctgtcagctg gattatcaat gatctcagct tctccatgca gactttccga gctctgcatg
    10921 agatctgtcc cttttccttt tgattcggtc aatattatcg ccttgatcaa attctcgtag
    10981 ttttccatat gataattttt tgcagctgtt gttatttcta tttgatagct ttccactcgt
    11041 tttaccgaac gataagttct tcctcataaa aacaacaccg caccaataac cgtcagaatg
    11101 atcatgtaaa aaagaatacg gataatagat aatcgttttc gcttcatttc atcacctagt
    11161 tattttgaca tcttttttta tcttactatt tctttatctt cttggctatt tcttccttga
    11221 tttctgtgta cgccttaaga aaaaatgaat tttcctgtat tttttgacat cttacgaaaa
    11281 tgatcgttgt ttttcagtaa aaacaattgc ccaatgactg atataatcgt tatactttac
    11341 acagtaggtg attatgtgaa aaaagaaaag aaatatgcaa aaatgccaga tgggagcgaa
    11401 atttactatg aaaagagcgg tcaaggcttc cctctctttt tactgcatgg caatgacggc
    11461 agcggccgct ttttttcaga acaggtccct gtactcgaac gctactatac cgtgtatcta
    11521 gtagatagcc gaggacatgg tcgttcgaca aacgaagcat ctatgttgaa ttttcagtta
    11581 atggccgagg atctgaatac gatcatgttg ctggaaaaaa tcgatcaggc agattttctt
    11641 ggatttagtg atggagcaaa tctagcactg gtatttgcca gcagcttccc taaaaaagtt
    11701 catcgattga ttttaaattc tggaaacacg cttgttaaag gcgttcgttt ttctgccaga
    11761 gtcatcagta atattcatta tgcatgggta tggttactct ctttatttcg cccaagtctt
    11821 cgcaaaaatc ttttagttat caaacttttg ctgcatgaca tcgggcttac agaaaatgat
    11881 ttaaaaaaaa tcaactcccc cactttgatc atcgtgggaa aaaaagatgt aatcaagcta
    11941 aaacattctt tgtatatagc taaaactata ccaaaagctt cctttgtgct tgtgaaagaa
    12001 caaggacatg aattagctag aaaagatcct gaacgcttca atcgagaagt tcttcaattt
    12061 ctttctgaaa cataactatt aggaatagaa ggtgatttat ttgttaaaaa ataccataca
    12121 atggctgaaa aaccacctag gactgttcaa aacaatcttt ttgatttctg tcattgtcat
    12181 tattgtcggt gaattgatgt ccatcggtaa aactctatct atccagcaat taggagaaac
    12241 gtttgccact atacctgtat ggaaaagcgg actaatgcta ttgattggtc ttatctctgt
    12301 gttacctatg gttggttatg atattatttt gaatcggatg cttgaacaaa agcagaatcc
    12361 tcgttacctg tttgagacaa gctggctgat caatacgatc aacaatattg ccggtttcgg
    12421 cgggtttgtt agtgtggggc ttcgctctga actatatgga caaaagaaag agagcagaaa
    12481 cgtgatccaa gcattatcga aagttttctt gtttttgatg gctggacttt ctatttacag
    12541 tctgctttct tttttacttg tagtcttcac gccagttgct ccttttttga agcaatattg
    12601 gatatggttg atcggcggcg gtctatattt tcctgtcgtc tttttattta ctactttcaa
    12661 aaagaaagga ttccttggtg gtacctcact taaaactcaa ggacagctat tgctagtttc
    12721 attgttggaa tggaccggtg tcctgtttag tttcctttct gttggttatt taatggaaat
    12781 ccggattgat ctgtggcaaa caattccatt attcattgct gcttcagtga taggaatcgt
    12841 gtctatgatt ccaggagaaa ttgggagttt cgatgtaatg atgattatcg ggttgtccgc
    12901 tattggtgtg ccaagagaaa tagtcgttgt gtggatcttg ctctatcgcc tgttctacta
    12961 catcattccg tttttgattg gaatcgtctt tttcttcaaa aatatcggtt ccacttttga
    13021 tcagcgttat tctggtatcc ctaaacaatt agcaacagaa attgcgcata aaatcgtggt
    13081 tgtcttatta tacttttctg gaatcatgct agtactttct gccactatcc cgcaagcttt
    13141 tacagagttt cgctggcttc atagtttgaa cccattgaaa tttcatttta ttatccaatt
    13201 tcctagtata ctattgggat ttcttttaat cattatgggc agagggatcg cagcacgtgt
    13261 gaaacgagca tatttgccta caatcttctt gattgttcta gctttacttt atgttttatt
    13321 aagtgatttt agtttcacac cggtggtttt cttgtctatt ctgcttctga tcattcttgc
    13381 ttctaaaaac gaattatttc gggaacaatt gatctattca tgggagtggc gtacgatcga
    13441 tggaatcatt attggtgcac tgtccttgct ttacatcgtt attggggtat acaatctgcc
    13501 ggatttccct catcggcgtc accattttat ttcctttttc ttatttcctt ctgaaaagat
    13561 ttggttttcg ggattgttgg ccatcatcgc tgttagtttc atgatcgttc tatttgttca
    13621 tttccttcaa ggagaaaaga aacagattgg cgaagccttt aatgaagaaa aggccctgaa
    13681 gatccttaca acttatggag gaaactccga tagtcaattg atcttcttaa aagataagcg
    13741 gatgtttgcc tatgaaaaag acggtgaacc aactgttctt ctccaattcg cttgcttcaa
    13801 taataaatgt attgtaatgg gtgatccttc tggtaagaaa gaagattttc cagaagcaat
    13861 cgaagctttt atcgaagaaa cagatcgact atgctattta ccagttttct acgaaacaag
    13921 cgaagagatt gttatgattt tgcatgaatt cggctacgat ttcattaaga tgggggaaga
    13981 agcttatgta gacctgaata gttttacaac ttcagggaaa aaaatgaagg gcactcgtgc
    14041 tgtcttgaat cggatcgaac gagaaggatt tacatttgat gtcttacaac caccattttc
    14101 tactgagcaa atgagtactt tcaaaaatat ctctgataat tggttgggtt cacgaaagga
    14161 aaaagggttt tctttaggct ttttctctga agattatcta caaagagcgc ctattgcagt
    14221 tgtcaaaaat acccaagaag aaattgtcgc ttttgcaacg atcatgccta cttacacgaa
    14281 caataaagta ggaacgattg atctgatgcg atatgattct aaaaaagcac cttctggcag
    14341 tatggatttc ttgttcttgc atttgtttgc ctatatgaaa gaagaacata tccaatggtt
    14401 caatttaggg atggcccctc tttcaaatgt cggaacttcc agaaagagtt ttttacagga
    14461 aaggattgca tacttggttt acgagtttgg ttctcatttt tattctttcc atggattaag
    14521 agaatataaa agcaagtacg ctacaaactg ggtctcacgt tatactctat attctagaga
    14581 tagttggatt gcatatgtta tgattgcttt gctgattatt gataatgcgc cagttgagca
    14641 gacacaaggt agaataagtg gtttgaaaaa atggatacga agaaagtatt aaaaactgca
    14701 taccgataga tgaacactct catgatgaat aaaagagact gaagtaaaac gaaagttcat
    14761 gcttctacaa tctcttctta tacaattgaa gaacaaagag cggtccaaac agaaatcctg
    14821 tttggaccgc tcttttattt taaacatttt tcattaccaa aatattcgag tcgcgtttct
    14881 tatgcttcga tctctgtaac gatacctgaa cctactgtcc gtccgccttc acgaattgag
    14941 aatgtcgttc cgttttcaac agcgattgga tggatcaagt caacatcaat cgtcacgttg
    15001 tcaccaggca tgaccatttc tgtatcttct ggcaatgtga ttgttcctgt aacatcagtt
    15061 gtgcggaagt agaactgtgg acgatagttg ttgaagaatg gagtatgacg tccaccttct
    15121 tcttttgtca acacgtacac ttctgctttg aattttgtat gtggagtaat ggaacctggt
    15181 ttagcaagta cttgtccacg ttcgatatcg tcacgttgga tcccccgtaa caacacgcct
    15241 acgttatccc cagcttcccc gtaatctaac gttttacgga acatttctac acctgttaca
    15301 actgcttttt gtgtttcagg tttgatccct acgatctcta cctcatcacc gacacgaaca
    15361 gcaccacgat caatacgacc tgaggcaaca gttcctcgac ctgtaattga gaagacgtct
    15421 tccactggca atagtaatgg tttatctgta tcacgttctg gtgttgggat atattcatct
    15481 actgtatcca tcaattccat aatagcagct tcagcatctg gatcgccttg caatgctttt
    15541 aatgcggaac ctttgatcac aggagtgtca tcgcctggga aaccatattc gctcaataac
    15601 tcgcgaactt ccatttctac caaatcgatc aattcttcat catcgactaa atcaactttg
    15661 ttcaagaaaa caatcaggta ttttacacca acttggcgtg acaataaaat gtgttcacgt
    15721 gtttgaggca tcggaccgtc agttgcagaa acaactaaga ttgcaccatc catttgtgct
    15781 gcccctgtaa tcatgttttt cacatagtcc gcgtgtcctg gtgcatcaat atgcgcgtag
    15841 tggcgttttt ctgtttcata ttctacgtgt gcggtattga tcgtaatccc acgttcacgt
    15901 tcttctggag ctgcatcaat actagcatag tcttgaggat ttgctaggcc ttttttgcct
    15961 aatactgtag tgatcgcagc agttaaagta gttttcccat ggtcaacgtg tccaatcgta
    16021 ccaatgttaa cgtgtggttt acttctgtca taatgttctt ttgccataat ttacaaacct
    16081 cctaaataat atagatcact gaaaaatttc agctctcacc tttttattat agtctttttt
    16141 tgactaaatc aaaaagaata cgtccgactt actttttgta agattcgaac taagcttatt
    16201 atacgtcaat attggtcaaa gtcaactaag agcactcgtc tgtcttatac gcgaaaaaag
    16261 taccttttat aaataaaaaa atgtgttttt tgagaaaaac ttcaagatag tccttaactt
    16321 tcttttgaag caataagaac aaaatgagga aaagtttaaa attcagctgt tgccaacaac
    16381 tttcaataga cgaaatcttc tatttcgtta ataataagga aagaggtgaa gtacatgaaa
    16441 aaattaattg gcgtacttgt cgttttaggc atatttctcg gcggaagttt tgcagcttat
    16501 cattattttt acggcggcga agcttattat acaaaaatca cgacgaatgg cgaaatgtca
    16561 gctacccaag cagataacgg agagaaattc acgacttata cctacaagca agcggcttat
    16621 aataaagatg gtgaaaaaaa acaggtgacg cttcgcgaag aaagagaaca tccattaaag
    16681 atgaatgcgt atcttaaact caaagtcaat cctcgtaaag gtgtattaag ctgggaagaa
    16741 ataaaggctt ctgaagtgcc taaaaatgcg gcagaaaaaa tcgataatta gtaaatggtc
    16801 ttaaaatttt attagataaa aagaggctgg gacagaagcg tttaactcca agaaata
//
LOCUS       DAXWOO010000049        16854 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.049, whole genome shotgun sequence.
ACCESSION   DAXWOO010000049 DAXWOO010000000
VERSION     DAXWOO010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16854
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.049"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(365..3070)
                     /locus_tag="ACGE70_001275"
     CDS             complement(365..3070)
                     /locus_tag="ACGE70_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFF8692922.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(3376..4323)
                     /locus_tag="ACGE70_001276"
     CDS             complement(3376..4323)
                     /locus_tag="ACGE70_001276"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFF8692923.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            4513..4782
                     /gene="rpsN"
                     /locus_tag="ACGE70_001277"
     CDS             4513..4782
                     /gene="rpsN"
                     /locus_tag="ACGE70_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFF8692924.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLHKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(4958..5320)
                     /locus_tag="ACGE70_001278"
     CDS             complement(4958..5320)
                     /locus_tag="ACGE70_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFF8692925.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(5331..6452)
                     /gene="alr"
                     /locus_tag="ACGE70_001279"
     CDS             complement(5331..6452)
                     /gene="alr"
                     /locus_tag="ACGE70_001279"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFF8692926.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(6468..6818)
                     /gene="acpS"
                     /locus_tag="ACGE70_001280"
     CDS             complement(6468..6818)
                     /gene="acpS"
                     /locus_tag="ACGE70_001280"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFF8692927.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(6948..8459)
                     /locus_tag="ACGE70_001281"
     CDS             complement(6948..8459)
                     /locus_tag="ACGE70_001281"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFF8692928.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(8781..10133)
                     /gene="murF"
                     /locus_tag="ACGE70_001282"
     CDS             complement(8781..10133)
                     /gene="murF"
                     /locus_tag="ACGE70_001282"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFF8692929.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLAATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(10286..11362)
                     /locus_tag="ACGE70_001283"
     CDS             complement(10286..11362)
                     /locus_tag="ACGE70_001283"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFF8692930.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            11558..13303
                     /locus_tag="ACGE70_001284"
     CDS             11558..13303
                     /locus_tag="ACGE70_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFF8692931.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(13583..14020)
                     /locus_tag="ACGE70_001285"
     CDS             complement(13583..14020)
                     /locus_tag="ACGE70_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFF8692932.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(14004..15377)
                     /locus_tag="ACGE70_001286"
     CDS             complement(14004..15377)
                     /locus_tag="ACGE70_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692933.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(15699..16187)
                     /locus_tag="ACGE70_001287"
     CDS             complement(15699..16187)
                     /locus_tag="ACGE70_001287"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8692934.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(16209..16811)
                     /locus_tag="ACGE70_001288"
     CDS             complement(16209..16811)
                     /locus_tag="ACGE70_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFF8692935.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         4734 a         3606 c         3065 g         5449 t
ORIGIN      
        1 ttagaaaatc aagttctatc ctttttgata tcttaacttt tggaagagag atgtaaggct
       61 gctttctacc catcattctt actactcgaa gcatagcgca gttgtcacaa cctcttgata
      121 aacggtgttt aaaagcttgc atctgcggta ataagctcaa ccaaattaaa aacataagaa
      181 gctgttttta tttggtcgtt cttattactt gatgcagagc agctttttta caacctcttg
      241 gtacacggtg ttcaatcagc tgaccctcgg tattagctca aaaaaacggc aaaatatgag
      301 gaactatttt tccatttttt tctctaatac tcaggtcaaa acactgattt cacaacctct
      361 ttttttaatg aaaaatatgg ctctcatgcg ccgcttcttt gaatttcgtc attttttctt
      421 cgatgaacgg atagattgcc gttcctacga ttccaaagac aacaaacaaa ccgatcatga
      481 tccagattgc tttcgtagca tttggccaat aaattcctcc tgcagattcc cgtaacaagt
      541 ttaccgcatg agtaaatggc aagaacggat tgatcatttg gaagaatttc cctgagactt
      601 ggatcggata gttcccgcct ccgccagaga tcgacagtac taagatgata atagcgatcc
      661 ctttaccgac attatcgaat aatgcagcca agacatagac gatcatcatg aacgctaagg
      721 cgatcaagac agcaaatgac acgctgtaga ccggattttg gacatctact cctagtaaga
      781 aaatattccc taatgtaacg atcaagcttt gcgcaatcgc cattgtcaaa aatgtcagca
      841 tacgagcaac gaattgttct cgtttcgtaa aattgtttcg gtctttcttt cctaaatagt
      901 attctgtcgt agcaacactt gaaagaagaa cagcgccgac ccacagacat agtgcagtat
      961 aaaacggggt gctagctgaa ccgttatttg cgattggata aagcgcattg gtttgtagct
     1021 caacaggttg tgtaaagaag tcactttctt ttgttgcgtc tagtttcaat aatttgatga
     1081 cttcgcctag atcgacttct tgttcgcctt ttcggatcgc gtctgctgct ttttggatac
     1141 cagctttgat atttggccag tcattgttga tcaagtccgc agcagcgttc acagctgttt
     1201 cgacttctgg cattttttca tttactgttg tcaatgtacc ggtgatttca ttttttagtg
     1261 ctggccaatc attttggata aaatcagcgg ccaatcctaa tttttgctga atgatcggta
     1321 attcattgtt gtataggtct gccccttcat tgatgccatt gacgatggta tccatatttc
     1381 cattcagcat tgtattggca tcatggattt cttgtccaat tgcaggcatt tctttttgat
     1441 atttttctaa aattgccact gcattcgtta cagttgcttt cgttgagctg agaagcgaat
     1501 caaagtcgat ctggtccgct ttgctcagtg cgccttgtgc agtcccaatc gtatcgatga
     1561 cttggtctag tagagaagtg acagtggaac tgatttgacc tgtattgata ccagcaatga
     1621 tgccattgat attattaaca acggcatcaa tttgatctag caattcatca atagccttcg
     1681 tgtcttcatt ttgaacagct gtgttcaaag cagataggcg gttttgcaga ttgcttagca
     1741 agggttttaa ttgatttagc tgatcaataa ttggctgtaa tttcgtattt ccagcacttg
     1801 tttgcaattt agtcaggaac tcgaccagtt tatcgattgc attttgttgt tgagatattc
     1861 cgtcaataaa gccgttcaat acattgctga tgtttttctt ttcatcatca gtcagcttac
     1921 catcttcaat agctgttctt acagtatcta ttaacgaacc tgtcgttgtt gccacttgtc
     1981 caattgactt caatgtaaca tctacactgt ttgtgatgct tggtatcgca tctttcagct
     2041 gagaggcacc tgattttgtt tggtttgcta gatcacttgc ctgattttct aaatttttca
     2101 catctggcaa gatatcttgt acttgttgga tgatcgtcaa gccttgtttt gcttcatcga
     2161 ttccttggtt cattgtctct tggatcgaag caaaatcgcc gtcgatctct gctagttgct
     2221 taccggcatt ttggatctct gggatttttt cttgcaaatc aaggatgatt tttgcttgat
     2281 ctttcaactc aggcattttg tcattcaatg caacgacttt ttcacccatc tcatctactt
     2341 tagggatgta atccacgaat tcatttgctt tattcagttt ttccttgatt tcaggtagtt
     2401 gactttgtaa ttcgaggact tgttttgtgt agccgtcgat cgtgtccaaa ttttcatctg
     2461 ttgataaaat catatcttta actttattga tacttactag gttcgtatcg atgtcgtaac
     2521 ctatctcatt gaatactttc aataaagtcg aactggctgt tttgataaat tcatttgtga
     2581 tttgcgattg gatcgaactt gctcctttat cggtgatttt cggtgcaatc gcattgattt
     2641 tttcatttac cgtatattcg atttttggtt tcttgatatc accactagta aaactcagca
     2701 gatcttctga gaaatccttt ggcaagtaaa tacctgcgta atactttccg gaacggactc
     2761 cgtcttctaa ttgttctttt gaatcaacga attgccatcc taattgttta tttttatgaa
     2821 gcgattcgat gacttgctcc ccaatggcca cttctttacc ttgaaactct gctggcttgt
     2881 ctgcactgta aacagcaatt ggcagctctc cagtatttcc atatggatcc cataatgctt
     2941 tgatgttaaa ccacgcatat agcgaaggga tgatcatgat cgcgatgatc agaagtgttg
     3001 caatcgggtt tttgaaaatc cgtttccagt caagcaaata taatcgaagc gtatttttga
     3061 ttgactgcat agttctcccc cttcttcttt tttcagctga attactttac catgtatttc
     3121 atactttttt caatcggctt ttttcaaaaa agtcgaattt ttggacatat cacggcaatt
     3181 gtctcattgt aatctcttac atattgagaa ataattaagg attacagcct ttactttatc
     3241 atgaaataaa agggaaagat aattttatgc caaagttttt atctattata cgtatacgca
     3301 aaaaagctaa aacatttttt gtctttgccc ctacaaaaat gtttcagctt ttttcgtttt
     3361 ttattcctgt atttcttatt tttcgaatga caggatgatt ttcccagcag gaggatgtcc
     3421 ttcaactagc tgatgtgctt ggatgacatg cttaagtgaa gcagacagat tcattgaaat
     3481 aaaaacatga aaggctcctt tttggtaggc tttgattatg ccagcaaagg cttctgcatc
     3541 tagatattcc tttcttggaa caaacgattc ataaaagcct tccttttttt gccgcaaatc
     3601 tagatctggg aggtcattta atgcaacata cctgccgcct ggtttcatga tctggatgcc
     3661 ggtttctcct ttgatacttc ctttcgtcgc atcgattaca aggtctgcct gatcagcaaa
     3721 ttgcagacct ggatttgttt tatcataagc agcaaatgct gaagctccta atttttttac
     3781 cttttcctca tttttactgg atgcactggt taagattcgg attccctttt catggagaag
     3841 ctggatcaga cttgacccga ccgcacctga tgctcctagt atcattacag tatctgtcgg
     3901 ctggatttcc gtcaaatggt tgattagatt ataagccgta attcctggcg tgaccattcc
     3961 ggctgcttct tcccaactca ttttatctgg aatttttgct acttttgctg atggcaacac
     4021 gacttcttct ccgtaagtcc cgccgactgc atggacagct actcgatcac ctacatgaac
     4081 agttgtgaca tctgaaccaa tctctgtcac aataccagcg ccatcattac ctggtacata
     4141 agggaatttc aatgttcgta tctctttcat ttctcctaaa cgcaaggcaa catcataagg
     4201 attcacactg aatgctttta tttctacacg aacatgtcca gaaccgactt ctctgggatg
     4261 agcatcgatt tcttcaaaaa catcttgtgc ttctccgtaa ccgttaattg caaatcttcg
     4321 catggtcacc atctcccttt ttctttaagt ttacgtttat gtctttattt tgacaaaaaa
     4381 aatgtctttt cgttttggcg catgattcaa aacaaaatag ttgtcttttt tcgcgtaacg
     4441 tgttaaatta tttatctaaa agcgtaaaga ttacgattta cgctaataaa aaataattgg
     4501 aggaatttgt tgatggctaa aaaatcaaag attgccaaag caaaaaaaca gcaagaaacg
     4561 attgaacgct atgctgcttt acgccaggaa ttaaaagcgc aaaaagatta cgaaggcttg
     4621 cataaacttc ctttagatgc tcgtccagaa cgattgaaaa atcgtgattt aatcgatgga
     4681 cgccctcgcg gatacatgcg taaattcggg atgtcccgta taaaattccg tgaattagca
     4741 cataaaggtt tgattccagg agtaaaaaaa gccagctggt aatccaagtc tggctgtaat
     4801 gtaaaggagg agggcgcatg gaccactcct tggatcatca aaaaagatac gttcgcttct
     4861 tctagcagaa atgctggtac tagaagcaga cgtatctttt tttacgcaca gctggtcctt
     4921 ttttatatgt aaaggctgca tggttacttc gctatattta tttagtaaca ggagtcagtg
     4981 cttcgatacc cacactaatt ttcaatgctc ggtcaacttc tgccataatg ggtcgatcca
     5041 aatggcagac cttttctttc aatcttgatt tatcgatcgt tctgatctgc tccaacagga
     5101 taacggagtc tctttctatt cctgttccag tagctttgat cccaatgtga gtcggaagct
     5161 taggtttagc catttttgcc gttatggccg caacaatgat cgtcgggcta aaatggttac
     5221 ctagattatt ttgaataacc aaaactgggc gtgttccgcc ttgctccgat cccacgactg
     5281 gagaaagatc agcaaaataa atatctcctc tttttaccat aggatcctcc ttattcttga
     5341 tattctcttg gtatccgctg tcctaatccg caagccactt catagtggat cgtacctaat
     5401 tgatctgcta tatcctgcat agtgatttct ttgtctgcat ttttgccgat aagcgtcact
     5461 tttgttccga caggaaattc ttgaggcaat cggatcatca gttgatccat acatacacgc
     5521 ccaatcgttt cacagtagtt cccttctacc aatagagaaa aaccctgcat ctttcgtggc
     5581 cagccatctg cataaccgat cggtatcgtt ccgatccact ctgcttcagg agtgatatat
     5641 gtttctccat agccgattcc ttcacctgct gggagttttt tgacttgaat caattcagaa
     5701 accagctcaa gtgctggctg caatggataa acttccggta gtgcgtgccc agagggatta
     5761 agtccgtaca ttgctactcc ataacgaatc atattgccga ttgtttcatc atgccataaa
     5821 gccgttgcac tattgctgac atgtacatat cgaggaagtt cgggaagttt tttcagcact
     5881 tctataaagc gttctttttg cagattccaa tatgtatcat ctgcctgatc tgctgtagaa
     5941 aaatgggtaa ataaaccttc ccattctaat acacgacttt ctttgatgaa tgcagctgcc
     6001 tcttttactt cttcaggtgt acaaaatccg atacgaccca tcccagtgtc tgcttttaga
     6061 tgaatcgaaa gaggcgtctc tagcttactt ttctctaaaa cagcagctgc tgcttgcaac
     6121 cattcccgtg tacctgcagt tactgataaa tcatgagcaa cagctacagt cagtgcgtcg
     6181 taaggaacca tattcaagat aaggatgggc tgagtaaatc cagcttctct caactcgact
     6241 ccttcatcta aatttgaaac acaaaagccc gaagctccac ctgcaactgc tgcctcagct
     6301 gtttcaatag ctccatgacc gtatccgttc gcttttacta ctgcaaacaa ttcttttcct
     6361 tgcggaagtc ttgcaacttc atttgctaca ttttcagtta tagctttttt atgtatcact
     6421 gctttcgttg ggcggtgcca agcgacaacc atcgtttttc cttctttcta gtcttccaaa
     6481 ataatttgtg caaaagctgt ttcatttgta tgagtaatac tgacccatac attgccttca
     6541 tgaggtgaac gcgtcacaac aggctgacca ttttcatttt ttaaaatctc gacatcttga
     6601 aaggtaactt ttccgatccc agttccccaa gctttagaga aagcctcttt gcaagcgtaa
     6661 cgcccgccta aaaattctac ttggcgatga aaaggcaagg actggaatag tttcatttca
     6721 tcagaggtca agattcgtgc aatgaatttt ggtttttctt cgatcagccg agtgatccta
     6781 ggaagttcga ctgcatcgat cccaattcct tttatcatat gcttttctct tttcaataaa
     6841 tttagaacac tctcattcta ccaaattata accattttgc cacaaataag tccacaaata
     6901 acaaaaatga aagtttctct taaaaaagag aaactttcat tttatgttta atcgttattt
     6961 gaacggatta caaatccgcg ttttttatca ttgtgacgtt taccgccatc tttttggctg
     7021 ttattatttt tgctataacc gccttttgat ttgtttccac gataattacc gccgtcacga
     7081 cgattacggt tgtttccgcc accacgtttg ccatttttat tgtatccttt tttctgcatt
     7141 ggcaatgggc gttctggtgt gatcttcact ggcactgcat cagctggatc ttttgctgtc
     7201 gtttttagca aaagtgcaac taaatcttgt gcagaatatt cttctaataa tttatctgct
     7261 gtttgcaggt atttatctag accattttct gcaagttttg tttcgatttg ttcaactgca
     7321 gcacctaatt gacctttgaa tgcttctttt tcggttggtg gacgtaaagt cgtcatccgt
     7381 tttttcgtca agttttcaat cacatgcaag tagtccattt catttggtgt aacgaaagtc
     7441 acagacatac cgccttttcc ggcacggcct gtacgtccga tacggtgaac gtagctttct
     7501 ggatcttgag ggatatcata gttgtacaca tgagtgacac ctgaaatatc taatccacgt
     7561 gcagcaacgt ctgtcgctac aaggatatct aaatgaccgc tcttaaatga acgcaatacg
     7621 ctcattcgtt tttgttgaga caaatcgcca tggattcctt ctgcacggta accgcgtgct
     7681 tctaatccgc gtgccaattc gtctacacgg cgttttgtac gtccgaagac gattgttaat
     7741 tcaggtgttt gaacatcaaa caagcgagtc atgatatcga atttttcgta ttcttttgcg
     7801 cggacatagt attgatcgat caagtcagct gtcatttctt tcgctttgat cttcacatga
     7861 tgtggatttt tcatgaattt aacgccaata ttcttgatag ctggcggcat cgttgctgag
     7921 aaaagtaatg tttgtctttg atcaggaact tgtgaaatga ttttttcgat atcttccaag
     7981 aagcccatat tcaacatttc atctgcttca tccaaaacta atgtttgaac ggttccaagt
     8041 ttcaatgtgt ggcggttaat gtggtcaagc atacgtcctg gtgtacccac aacgatatgt
     8101 ggacgatctt tcaagccacg gatctgacgg ccaatatctg ctccgccgta aacggcttgt
     8161 acacgtactt ttttgtcgcg ccctaaacga tataattctt cttgtgtttg gatagctaat
     8221 tcacgtgttg gtgcgataac taaaccttgt aattcgtgac gatctggatc gattttttct
     8281 aacattggta atccaaatgc agctgttttc cctgtacctg tctgtgcttg tccaataacg
     8341 tcttttccag ctaatgctag tggaatcgtt tctgcttgga taggcgttgc ttcttcaaaa
     8401 ccagcgcgtt cgactgattt caacaattct ggtgataatt ctagttcttt aaatttcaaa
     8461 tgaatcctcc tatatggttg ttaactgagg atgtagcttt tttgctaccc cgtcgatttg
     8521 ttcggtcaaa gtgtattgtt tctttttgac agtggccacg cacgttttta tccattctta
     8581 gcccctgtgg caccaccgaa aaatcacttt gcgaagttta ggcattagaa aagccaaacg
     8641 ggtcgcgttc agctttatca ttattatgac atctggcttc aacaagttgt ttctatggca
     8701 aaaccagtcc aatttctctt catctttcgg caataggtta ttctaacata gtttttcgtt
     8761 ttctgcaagt ttacagattt ttatttcatt tgctgaagtt gttctaccac ttcaaataag
     8821 cccatgctgt tgctgccttt tagcataata ctatctgttg ctgcaagatt ttgtttgata
     8881 gcatcaatca atttattctt ttctttttca aaatagaaca caggtagttg ggggtatttt
     8941 tggttcaatt cttcttttag tgcatgcatt tgttccccat aaaggaaaat catggcgaat
     9001 ttctcaggat cgatatgttc actcatttgc gcatgcatat ttaaagactc tttccctaat
     9061 tccagcatat ctgctaacac agcgattctg cgtccttttg ttggcagatt tttaaaagta
     9121 tctaatacca gtcccatggc tgttggattt gcattgtaga catcgctcaa aatagctgca
     9181 ccatttcctg ccgttagcca ttcggttctg ttttgagtga tagaaacatg ggcgagccct
     9241 tggcgtatct caggtatgga cagtccaaag aagcgcccga tcgtatacgc aatcaaagca
     9301 ttcgttacat tataacttcc aggcagcggg atcgtaaatg tttctccttc tactacaaaa
     9361 tcggtttgtg cttttccttt tgctgtcact tccgcatgaa catcgccatt tccaataccg
     9421 aatgttgtga ctgtttgtac taatgttgcc gttaaaggtg tcagcaacgg ttcatctgcg
     9481 ggcacaacga gtaatccgtc tgatacaagt ccgtcagtga tttccatttt ggctttagcg
     9541 attccttctc gcgacccaag attttctatg tgtgcttcac cgatcattgt gattgctgca
     9601 gcttttggtt gagccataag tgaaagttcc gtgatttcat tggcgtgatc catgcccatt
     9661 tccaaaacga tcttttctgt atcttggggc atgtgcaaaa tcgtgtaagg aagaccaata
     9721 ttattgttgt aatttccttg cgtcttatat gttttgtatt tttcagacaa gacagacgcg
     9781 gttagatctt tcgtggtggt tttaccattg cttcctgtaa ttgcaacgac ttctgcgcct
     9841 actttttcca agtaataggt tgccaattgc tgcatggctt tcaaaggatc tgctacagga
     9901 ataactggaa aattttttgg agcttgttca acaggcaagc tccaaaatga agcagatgct
     9961 ccgtttgtga tcgcttgctg gataaattca tgtccatctc gcgtaccagc aagaggaacg
    10021 aataaacttc cttcggttat cttccggctg tcaaattcga cattggtaat ctttgtcagt
    10081 gcagagcatt cgctgcccac ggcttgtgca atttcttgta ttgttagatt cattgagatc
    10141 tctcctaaaa aaggttgtga aaaaagcgtt tggcctaaac ggcgggttcg catgactctc
    10201 acttccaagt aaatttctct attcgtattt agaagctaga atgaacgcgg ttacgcaatc
    10261 actccagcct cttctttatt tctctttatt catttttttc aaaaaaagat tgacgctgat
    10321 ggtatcgatt cattcctaac tggatcagtt cttcaatcaa atcaccgtat ttcaagccca
    10381 tattttccca taagagtggg tacatactga actccgtaaa tcctggcata gagtttaatt
    10441 cattcaggaa taattcattt ttatttgtca aaaagaaatc gcaccggctc aatccgcttc
    10501 cacctaacat cgtgtaagct aacttcgcgt actcttgcgc tttttgataa acttcttctg
    10561 gcacttcggc tggaatctgc atttcgattt tattattgat atattttgct tcataatcat
    10621 agaatgctac gtcttttacg acttcaccag gcaaagtcgt ccgaacgtct tcatttccta
    10681 atacagcaac ttcgatttcg cgcgcttcaa ttccttgttc aacgattgct cgagaatcat
    10741 actgataggc tgttgctaaa gcattttgca gctcttctcg gttttctgct tttgtaatgc
    10801 cgacactaga acccatattc gccggtttga caaacatcgg ataaagcaaa gaaccttcac
    10861 attgatcaaa tactttttta ggattttctt tccattgatt cttaagtact ggtacataag
    10921 gaacttgcgg cacaccagca gcttgtaaaa tatacttggt catgatttta tccatggcac
    10981 atgcactggt caatacgcct gcgccgacat aaggcatatt caatgtctct aagaagcctt
    11041 ggatcgttcc atcttccccg tttggcccat gtaaaactgg aaaaacgatg gctccttctt
    11101 ctttgatttc gcccggattg atcacttttc ctgtaaagcc ttcctctgtc tgtccacttg
    11161 ggtcccatga aagatgcaag acatctttgc tggcaggttt ttctgttagt aatggacctt
    11221 tgacccattg tccttctttt gtaataaata cgagttgaac ttggtaataa ttataataaa
    11281 tggcatttaa aactgaaaat gcggaaagaa tggacacttc atgctctgcg ctgcgtccgc
    11341 catatagtaa agtaatcttc aaaagattgt cctcctgtat tctaaatcgt tcagtgattt
    11401 actctcatat tttagcacaa aacaaaaata aacgaataac ttatttataa aaaaacgagc
    11461 cagcttctaa aagaacgagc gtcatttatt ttttgtcagc tagattattt gtcccgtttt
    11521 ttataaaaag ttatgataaa gaaaaggagg gatgatgatg gcgcaaacaa atccacgcag
    11581 ccgcttggca gaaatcattc gagtgttcat caattacaat gtagtgccaa actttgtaca
    11641 gcaaaaaaat cccgaacagg taaaaaaagc ctttgaagaa ctaggcccca ccttcatcaa
    11701 gataggacag atgctttctg ttcgagaaga tttgctttct tctgccttca ctcaaacatt
    11761 caaaacatta caagatagtg tacctagcga cacattttca acggtcaaaa agacgatcga
    11821 aacagaactt tctctttcat tatcagatat attcgatgat ttttcaaaat cgccttttgc
    11881 ctctgcttct atggggcagg ctcatcgtgc caagctgaag aacggtgacg cggtagtcgt
    11941 taagatccaa caccctaaca tcgccgaaga gatccgactt gatcttcaac tatttgaacg
    12001 agcgatccct ttgatcaaat acattcctga aactagcgta gttgatctaa aaggggtact
    12061 ccaagaagta aaacgttcct tgatcaatga aatggacttc ttaaaagaaa gtcaaaatgg
    12121 cgaacaattc tatcaaaaga acaatggctg gaaagagatc cgcagcccca agatttatga
    12181 cgctttttgt tcgaaaaaag tgatcgtgat ggaagaaatg tctggtaaaa acttgaatca
    12241 tttaatgaac gccgaaaaca aaactgaaac ttttatcact ggaattcaaa acaagcagct
    12301 gaaacaagaa gttgcaaagc ttttagttga gaactttatg aaacaggtgt ttgatgatgg
    12361 ttttttccat gctgatcctc atccagggaa tctgctattc catgtactaa caaaagaaga
    12421 acagacacaa gctagccgaa aaactgaaac agtccatgaa aaagagttcg gttcctttgc
    12481 ttttcgagcc tccacttctg cagaagaccc tgttgcgcca tataccatca actacatcga
    12541 ttttggtatg atggggcatc tttctgcagg acttaggcaa aaactcacac aagccgtttt
    12601 agcgctgtat acgaaagatg cttatcgaat cgaaaaagcg gtccttcgac tttgtcagca
    12661 agaaggctcg tttgatgaaa gtcggttcca ccaagaactg acaagttttc ttgaacagta
    12721 ttatgattct cctattgatg aaatcaactt gcaagaagta ttcacacatg tcgtgacgat
    12781 ttgtcatcaa aataatctgc agttcgatcg tgatatcacg cttttgctga aagcatttgg
    12841 gacattagaa ggggtgatcc gcgtcctcga tcctgaagtt tccctcatgg aagtcgcttc
    12901 gccttttgct caacattatt ttctcactca tctggatgta gaagatacat tgaaacaaag
    12961 cggattagac ttattggaag ggatgaaggc tgcgcctaag atccctcaac agctccacca
    13021 tctattagaa atgtggactt cgggacaagg aaaagtcaat ttggaattga aaaagcaaga
    13081 caaattattg agcaggatcg aaagtatgat caatcggctt gttttcggta tgattttagc
    13141 tgcattgatc gtagggtcct ccctgcttgt ccaagcagca cctgtagaaa acgcagaggt
    13201 cgtttcctta ttagggatat ttacgtatgc tattgccgca ttcgtcatta tttttttggc
    13261 aatcgatgca ttgatccaaa tgtataagaa gaggaaaaag tagaaaaagg ttgtgacaga
    13321 aagaatcagc ttcaagaaat aagaaggggt tttacataaa ttgtttctca aatttttgtg
    13381 aaaattcggc ttatttttcg aagaggctac ttctgaaaca ccgagaataa aaaatgtgaa
    13441 aaagctgttc tgcacccagt aataagaaca atcaaaaagg gaacatggaa cacatttatc
    13501 ctgtttccct gttccctttt tgatgtattc gagcataaat aaaatacctt cgttacctac
    13561 tatcttctgt acttggtaaa ccttagtcaa ttgccttttt taacgcttga cgaaaagaca
    13621 tatgatcttt ctccgtaaag ggttttggac cctttgtcct tatgccagct tttcttaact
    13681 ttccactatg ataacgttgt tctagaagcg tgccgatatt cgcttcgtca aattcttttc
    13741 cagagtgatg gaacactcgc acacccttcg gcaatacaag tgaagctaaa ccataatctt
    13801 gcgtgatcaa aaagtcattc ttcttaacca taccgacgat tttataatcc gcactatcag
    13861 ctccgcggtc tacgtagacg atttggacat gattagcgta ttctttggtc gtataatggt
    13921 ccacactggt cacgataacc acgtcaagac cataggattc tcctaatttt atgacatctt
    13981 cttttactgg agaaccatct ccatcaatga atagcctcat tctttttcgc cttcttccat
    14041 aaatgataaa cagttactaa tccgctcagt aaaagaccaa cattgatcca ttttgccaag
    14101 ataagcagtt gtacactttc tatcggcact gattcatggt tcagcgtgat caataataga
    14161 tggattggct ctcccatttc ttttgctaaa acaaacaaaa gcgtcatcaa taaaaactgg
    14221 tggatcacac tccctttatt ttgagtagta tacttgacaa cgatccggcg atccattgga
    14281 atagctgtat cagtttgata atattcctta tttagccagc tgcctgttac tacgtgccag
    14341 aaactaagca gaaagacacc tatcgcaaaa ccatttggaa tcaagaggac caataaagaa
    14401 gaactcagtc caagcaattg catagccaat ggagaccatg ttaaacgttt caaaatccat
    14461 ggtccgacta tttttgctgc aatcaagcct gctgcataag gcaaatacat aaaaatcgaa
    14521 agtttttcta atccgtacag tatgccaaca taaaaactgc taaataaaaa aaggaaattc
    14581 cctagcattc catttagaaa agtaaacaga tttagccagc ttggcaactt ccattccttc
    14641 ttctggtgtc ctaaaacaaa cacgaacaag atgaacagca gcaagaaacc ataaatagca
    14701 agatcaaaaa gcctttcatc gaataacaat cgggcatttc tcaaaagaaa caataagcca
    14761 aagaatatca aaaataaaac aagctctcgc gtagctacca gatttctttt gatgtctttg
    14821 aaatccagtt cgtaacgcgg ataatgacta accgtatggt aggccatgac gtaaaaaagt
    14881 gtataaatag taaagcttgc tgcaatctga gtcggtgcag gcaactcaat cgcttgatac
    14941 aggagaaata gccagatagc tgcaaataag tactttttcc catccatatt gataaaacct
    15001 tgcgtctttt cgtgaaaatt gaccgtggta ttagcaggta caagccatgc tgcactcagc
    15061 cctagcaaaa cacttgaaaa ataaaataaa ggaaaataaa atacgcctaa tattccaagc
    15121 aatgaaccag caccaccaat cagcagagcg atcatcagca acgtgtaact atctagacca
    15181 aatcgtagac tgttgactaa aaatagtcca gtcatccgaa aggcataaaa cagcgtaaat
    15241 ggcaaaatcg tcagccaatt atgcgagtct gttagtccca aaaataaaag ataaggaata
    15301 aaaataaccg tgttacttaa gataaagggc gttgtttgtg taaaattacg cccaaatcgt
    15361 ttgagttttt cgaacatgac aatggctcct taaacagtct tataactaat ctgctcttcg
    15421 attttagcat taaaggggaa ataaaccaat tttacagaat atattcttgt aaaaaaaaat
    15481 acagaaagaa gcataagctt ctttctgcat ttttacggta cttgattact caagcagaca
    15541 agcccttctc cacaaaagtc ttgcccgaca cccctgcatc cggcaggtga taaagataga
    15601 aatcaattat cctgtgcctc caaaatcaat taggagctct gtaaaccaga gtccttctcg
    15661 acccgtcgca taaatttact ttaacacagt tcctatgttt atgcgagagc ttttttcatt
    15721 ttataatcga ccatttgaaa tccttgaccg atatctgaaa taagttcttc tgtaatctca
    15781 aatccttcat gcttataaac ctcgattgct tgatgattcc cctgattcac tcgcaaaaag
    15841 atctctcttt cctttgtttg cgcagcaagt tcttcatacc agcgaaaaac tgaagaagtc
    15901 aatccttgac cacgtacttt atttagcaaa tataatttac ttaaataaat ttttccattt
    15961 atctcttcat aagcagtgta accgacagtc ttttcttcgc tttttaataa aaagtaggac
    16021 acaccttctg tcagttcttt ttgaatctgt ttttttgact gataagtttt cagcatgtaa
    16081 tccacttgtt tttgaccaat gatcggcaca aaagcttccg gccatacttc ttgtatgact
    16141 gccatcagct catccagttc attttctgtt acttttattt tttccataca cccacgtcct
    16201 tttttacatt ataaatgacc ttgcgcttga tggaataaaa agagcatgat catgctatac
    16261 acacccaccg accatggttt caagaaaatc acaatctgca taaattccct aaaagataat
    16321 ttcgtcaaac cagctactag acagatcaag tcgtctggcg caaagggaag cagcaaggta
    16381 acaactagta cttttttaat atctttcgtt tttcttgtca gcaattcatc aaatttctga
    16441 tacttttttg gcgataaaat aagctggaca aaggctacgc catagtaacg gacaagcaaa
    16501 aatccgattg cttccccaat cactaggcca atacaactat ataaaagccc aaatccattg
    16561 ccaaacatca gcatgcccgc tactgaggag atccctcccg gcaaaatagg aatgatcact
    16621 tggatcactt gcaacagaac aaatatcatc actgcatact tgccaaattg ctggataaac
    16681 gcttgcaaag acgtgctcga tcgaaaaatc ccatgatgat aagcataaag aatcaacaca
    16741 aagaaaaaaa acagaccaat aatgggcatg atctgaagac atttttgtaa aactgatcct
    16801 tttttagaca tctatattcc ccacatcttt tttcatttat taaaactgcc tcct
//
LOCUS       DAXWOO010000050        16632 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.050, whole genome shotgun sequence.
ACCESSION   DAXWOO010000050 DAXWOO010000000
VERSION     DAXWOO010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16632
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.050"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(46..780)
                     /locus_tag="ACGE70_001289"
     CDS             complement(46..780)
                     /locus_tag="ACGE70_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFF8692936.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRFVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1106..1516
                     /locus_tag="ACGE70_001290"
     CDS             1106..1516
                     /locus_tag="ACGE70_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFF8692937.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     EGKTAEGVEALLGYVRKKKAGRVSVNEMEENQID"
     gene            1509..2210
                     /locus_tag="ACGE70_001291"
     CDS             1509..2210
                     /locus_tag="ACGE70_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFF8692938.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2210..3823
                     /locus_tag="ACGE70_001292"
     CDS             2210..3823
                     /locus_tag="ACGE70_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8692939.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKKTIKTITKKEKKDDI"
     gene            3813..4034
                     /locus_tag="ACGE70_001293"
     CDS             3813..4034
                     /locus_tag="ACGE70_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFF8692940.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4031..4792
                     /locus_tag="ACGE70_001294"
     CDS             4031..4792
                     /locus_tag="ACGE70_001294"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFF8692941.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEELSLRFPETFAYALGDVRQMKELQQAVSKCQDRFGEVTILLNTSGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRRKSGTIINMASIAGLVAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5173..5682
                     /locus_tag="ACGE70_001295"
     CDS             5173..5682
                     /locus_tag="ACGE70_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFF8692942.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANMASPLGLIDVVVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5752..6291)
                     /locus_tag="ACGE70_001296"
     CDS             complement(5752..6291)
                     /locus_tag="ACGE70_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFF8692943.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVVVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6431..7042)
                     /gene="rpsD"
                     /locus_tag="ACGE70_001297"
     CDS             complement(6431..7042)
                     /gene="rpsD"
                     /locus_tag="ACGE70_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFF8692944.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7304..8155
                     /locus_tag="ACGE70_001298"
     CDS             7304..8155
                     /locus_tag="ACGE70_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFF8692945.1"
                     /translation="MRGHVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPAATILVEGMFANIVVNTAVLISMRMKEDAGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLAYFASQGQTDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8183..8818
                     /locus_tag="ACGE70_001299"
     CDS             8183..8818
                     /locus_tag="ACGE70_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFF8692946.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVYYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8838..9611
                     /locus_tag="ACGE70_001300"
     CDS             8838..9611
                     /locus_tag="ACGE70_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFF8692947.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10019..10816
                     /locus_tag="ACGE70_001301"
     CDS             10019..10816
                     /locus_tag="ACGE70_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFF8692948.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKKIQLIQINDDRPFME"
     gene            10794..11207
                     /locus_tag="ACGE70_001302"
     CDS             10794..11207
                     /locus_tag="ACGE70_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFF8692949.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEQEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11191..13836
                     /gene="mutS"
                     /locus_tag="ACGE70_001303"
     CDS             11191..13836
                     /gene="mutS"
                     /locus_tag="ACGE70_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFF8692950.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLKKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKNSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13854..15962
                     /gene="mutL"
                     /locus_tag="ACGE70_001304"
     CDS             13854..15962
                     /gene="mutL"
                     /locus_tag="ACGE70_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFF8692951.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEEPKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPVV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15984..16544
                     /locus_tag="ACGE70_001305"
     CDS             15984..16544
                     /locus_tag="ACGE70_001305"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFF8692952.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARIVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
BASE COUNT         5399 a         2970 c         3602 g         4661 t
ORIGIN      
        1 cttggtacgc aatcgctgta ccaagttttt ttatttagct tttgattaag ccataaataa
       61 taatttatat aagaatactg ctacgacacc agcacagata ggtgctacaa ctggtaccca
      121 tgcatatccc cattgagaag aacctttatg tttcaatgga agaagtgcgt gtacaaaacg
      181 tggagcaaag tcgcgggctg ggtttagtcc aggacctgta ggaccaccaa gtgaagcaac
      241 taatgtccat acaaggaaac cgagtgctaa gtgagctgta ccaagattat cttggaagaa
      301 tggtgctttt gtaataccta aggcaccaaa gaataaaacg aatgaaccaa agaattcatt
      361 gacgaaacca ttgaatttag aaccaacgct gttgattgtt gagaatgtac ctaacacatg
      421 gttcacgtct tctgtttggt cgtagtaagg tttgtaacaa gctactacga tcaattgtcc
      481 aatcgctgca ccaatcaatt gtgcaatgat ataaggaaca acttcagccc aagggaatag
      541 accattagat gctaaagcaa ttgtaaatgc tgggttgatg tggttaccgc tgattccacc
      601 aaaaatcatt gctggcatca taaccccgaa tccatacccg acagcaatca gcatccagtc
      661 actgccatat ccttttgtac cttttaaatc tacgtttgct acagcaccgt tacctagaac
      721 gattagtaat gctgtaccaa tcatttccgc cgcaagacgg gtcattagtg agtaatccat
      781 aaatacctcc aaaagttata aaaatgcaaa aaaaaagaaa catcaacacg aaaagcatcg
      841 gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat aaaaaaatga
      901 attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt taaaagttct
      961 ccacaaacca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt tttctcaaaa
     1021 attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta gtaaaatgaa
     1081 agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca ttattatttt
     1141 cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc ctggaagtat
     1201 cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc ctgaaaaaat
     1261 cggagcaact ggtgattttt tattgaataa tctagctatt ttatttgttc ctgctggtgt
     1321 aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac tgggagcagt
     1381 cgtcgtgtgt tccatcgtca cgatggttgc agagggaaaa acggcagaag gtgtagaagc
     1441 tttgctgggc tatgtccgta agaaaaaagc aggaagggta tctgtaaacg aaatggagga
     1501 aaatcaaatt gactgagttt tttgtgaatc cactatttgg cttaagtttg actttaggag
     1561 tgtatgtatt atctgataaa tttttgcgga aatataaact gcctgttttg aatcctctcg
     1621 tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc tataaagact
     1681 ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg gcacttgcca
     1741 tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta atggctgcga
     1801 ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac ttcttctctc
     1861 ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct atttctgtcg
     1921 atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt gtctctacag
     1981 gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc gaaagtccag
     2041 ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat cggtacagca aaagcaatcg
     2101 agattggcga aatagaagga atcatttccg gacttgcgat atgcgtaaat gggatattga
     2161 ccgttttgtt gctgccgtta ttctttcagc cttttgctgg cttgttttga tgcgaaaacc
     2221 actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga aagacgtgta
     2281 tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga ttctcctcct
     2341 agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag ttgttcagct
     2401 ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc agcagtttag
     2461 cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact tttatgatac
     2521 gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta cgctgaaatt
     2581 ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat ttgcgcaaaa
     2641 gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac agcaactgca
     2701 aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag aagaaatcat
     2761 tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct ggccatttga
     2821 aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt tttttaatgt
     2881 tcgtctgaac gagatcaaaa aaagacgatt ggaatacatg ctaggtgcct tttttttaag
     2941 aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga tttctgacaa
     3001 tctgttgttc cagcgatttt gccaagtcat ggagccggta tttcctaatt attttcaagt
     3061 agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat attatagtga
     3121 tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac cgccctttac
     3181 agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg ggctgccagc
     3241 agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt tcgcctatct
     3301 ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta atcatttagt
     3361 tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta gacacaaaca
     3421 tcccaatcat ctggcattta aaaatcatgc gtttttgatg ggtcggtatc tgactgtgtt
     3481 taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt taatgacaga
     3541 tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt tccgtaatga
     3601 gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc ttctgatttc
     3661 gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg tgaccgaaga
     3721 actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc aaaaaaaaac
     3781 tattaaaaca ataacaaaga aggagaaaaa agatgacatt tgaagaaatt ttaccagaat
     3841 tgaaaaaagg tgcaaagatt attcgtaaag gatggagtgg atttgaactt tatgttgttc
     3901 tcgtatccga tgatgaattt gatggctgtc cagtgacacc ttacttattg atcaaaacaa
     3961 gtgacgaagg tttttccagc ttcgctccta cagtttgtga tattttagca gatgattggg
     4021 cgatcgtaga atgaaaaacc agtatccaga tctagctaaa caggttgtat ttatcacagg
     4081 agctgcttct ggaattggtg ctgcgcaagc ccgagcgttt cttgaacaag atgcttttgt
     4141 atttggatta gatcaaaagt tcggtaaaat ggaagaactc tctttgcgat ttcctgaaac
     4201 ttttgcctac gcactaggtg atgttcgaca aatgaaagaa ctccaacaag cggtatcgaa
     4261 atgccaggat cgttttggtg aagttacgat tttactgaat acatcgggga tattggatgc
     4321 ctacaaacca ttgatggaaa ctgacgaatc gctgtgggat cttatttatg agacgaatgt
     4381 gaaaagcatg tatcagctga caaaaatcat ccttcctgat atgatccgcc gaaaaagcgg
     4441 aacaatcatc aacatggctt cgattgcggg tttggttgct ggcggaggtg ggatcgctta
     4501 tacaagtgcg aaacatgcaa tcgtcggatt taccaaacaa ctcgctctgg attacgcagg
     4561 acagggtatc cgagtaaaag gaattgctcc aggagccatc caaacaccaa tgaatgcggc
     4621 tgattttgaa gggaaaggtg agatggctga gtgggtcgca aatgaaacac cagtcaagcg
     4681 atgggcacag ccggaagaag tagctgagct gacgttattt cttgcctctc ctcaagcaag
     4741 ctacatacaa ggaacgatca tcccaatcga tggcggatgg ttgttaaaat aaccttgtgt
     4801 ttttttcaag cttctgtatg ataaaactaa agagagaaac aacggttgat gataaaagaa
     4861 agtcgatgac tgttcccgac attcgatgcc gaacagccag ttcaaaaaat tctttttaat
     4921 aatcgttaat attcgaaact aagcggcttt tcacgacctt ttgatataaa cggtgttcaa
     4981 aagcttgcaa ctgcggtaac aagtccagcc aaatgaaaaa tataaggagc tatttttatt
     5041 tgactattct tattacttgt tgcagggcag ctttttcact accttctgcg gcacttcagt
     5101 tggaattttc ttcccatcct ttggtcgcag tcattaccaa gataaaatta tcttgcaaag
     5161 gagaaataga acatggaaaa aaataatacc cgttcagcca aacgctggac aacgacagat
     5221 gtgactcgga tggcgctagt cactggttta tatgtggcag tgacgctggt tttgtctgtc
     5281 tttagctttg gagtgattca gattcgtctc tcggagatgt tcaactatct gtccttgtat
     5341 aacaaaagat acatcgtcgc ggtaacagcg ggagtagctt tggctaatat ggcttctcct
     5401 cttggactga tagatgttgt agtaggaagc gtcagcacgc tgcttgtatt gcttatcaac
     5461 tataaaatca ctagtcgcat caaaaacatg aaaatcaaaa tggctgtgac agcatgcgta
     5521 tttgcttttt ccatgtttac agtagctggt cagctaacga ttttatatca actacctttt
     5581 ttcttgaatt ggtgggtgat tgctttagga gaattggcat caatgattgt cggtgggata
     5641 attatttatt ggatcggtaa aaaagtggat ttgtcaaaat aaagatctgt ttaatttaaa
     5701 atatggcagc gtggaagaga ctttgagtct tcttccatgc tgcttttttg attaatttgt
     5761 atgaagaaat cgatttagcc gttttttcat caagataccg atataagctg ctccagttgc
     5821 cttgattgct tccggcagta aaaacggcag tagtccactg gcaaatgcag ttgtaaaagt
     5881 aagatcacca cttattttca gccagatcgt tccgcagatc aatgcagcaa gagagcccat
     5941 tagattggca aagattgcat ataagtaatg agcatccgtt ctttcgatca tccaaccagt
     6001 tatccacgtt tgtaagagaa atccaatcaa atatccgcca gttggcccaa ataaaacagc
     6061 tatacctcca gtcatccctg aaaatacagg aagaccaatc aagccaagta aaagataaac
     6121 tacaacagat atcaatccta gttttcttcc gagaaaagtt gctgttaatc caatagcaaa
     6181 tgtttgcccc gtcaaaggaa ccaagccaaa aggaatggtc atctgagaaa ggacagaaat
     6241 aatcgcggca aattccgcag caagtattaa ttctttcaat gttcggttca taatgccctc
     6301 ttttcgttaa cttaatattt ttatttggtt aacgaaattg taaaacaaag tgattacttt
     6361 gtaaatagaa aatataaaaa aagaccagct atccaataga tagcggtctt tttcaagaaa
     6421 gcttttgaaa ttataagctt tggttgtagt attcaacgac aagagcttcg tcgatttctg
     6481 gatatagttc gtcacgttct ggtaaacgag tcaatgaacc ttccaatttt tcagcgtcaa
     6541 agcttacgaa tgctgggcgt ccaacagttg cttctacagc ttcttttact gttacgatgt
     6601 tttgtgattt ttcacgaact gagatcactt gacccacttc tacgtggtat gaagggatat
     6661 caacgcgttt gccatcaact aaaatgtgac catggttaac taattgacgt gcttgacgac
     6721 gagtcgttgc aagtcctaaa cggtaaacaa cattgtctaa acgttgttct agtaagatca
     6781 tgaagttaac accatgttta ccttctttga ttttgctagc ttttacgaac aagttacgga
     6841 attgacgttc gttcattccg tacatgtgac gaagtttttg tttttcagtt aattgcatgc
     6901 cgtattctga ttgtttgcca cggctgttag gtccatgttg acctggtgcg taagggcgac
     6961 gtgctaattc ttttcctgtg cctgacaatg agatgcctaa gcgacgagaa attttccatg
     7021 atggtcctgt ataacgagac attaaaattt cctccaatga atataagttt ttggagtaaa
     7081 ataatccgtt gaaaaattca taatcatata gtttgttctt caatcttcac ctttgcagcc
     7141 gcggttacac aatcgaaccc gagacggggc aacaaactag ggatgagtta ttatttttct
     7201 gctgcattat tttacacaaa ctacagtata tgatagatca tccacgaatg tcaagataat
     7261 tagtacataa aaacgcttta caaacctagg ctgtctttat aaaatgagag ggcatgtgca
     7321 aatggaaaaa agcgaaggaa gtttaaggag aaaatcaatg aatccggtat caccattatt
     7381 tgagaaaatc gatgcttcaa ttgaaaagaa ggcgaatctt tttgaaaaca gcttttcacg
     7441 ttacgctgtt cgagcggttc tcgcctgttt gtttttgact ttaggaacgg ctgtggcatt
     7501 tgcaatcgcg atgaaggggg aagatatcgc gcatggtcta ggaaagattt tatacgcatt
     7561 tatgttcagc tggggtctgg tcatgattct atacatgaac gctgaattag gaacatcgaa
     7621 tatgctttat atgaccgtgg gtgtttttcg gcagagactt gctttaagta aggcagccaa
     7681 gattctattt gcctgtattt ttttcaatat gattggcggt atattatgtg gatttcttat
     7741 ttctatgact acgccatttc aagatttaac gttggacaac tttatgttag aatcaatagc
     7801 agtaaaacta gcaaaacctg cagcaacgat tttggtggaa gggatgtttg cgaatatcgt
     7861 tgtcaatacc gctgtactga tcagcatgcg gatgaaagag gatgccggga aagtgatcac
     7921 agtcgtattc atcattttca tttttgcttt tttaggatat gaacacgtga tagcgaattt
     7981 tcctgccttt acgcttgctt actttgcttc tcaaggacag acggatggaa tgacagtcag
     8041 caatgtgtta cataatttgt tttttgcttt ggcaggaaat tatattggtg gcggtttagt
     8101 aatgggactg ggatatgctt ggctgaatca gtcaaaatca tcctatgtgg actaaaaagt
     8161 cgttcattaa aggggaagtg ccatggaagt tttagttttt ccaaaaaatg aggaaaaagc
     8221 gttacaaatg gaaaagtaca tgaaaaatct tttttccttt gctggagtac ctaagccgga
     8281 acgcgctctt ttacaacaac cgttactgga tgagagcaaa aatttatcta tttctgaagt
     8341 tttagctttt gtttattatt attatgaaca aatcgaaaga gagtatcagt atgtagcgat
     8401 cgatttagtc cagaaaaaca tcaaacggct tacatttgaa gacttagtcg tattgttgcc
     8461 actagttcag aaaaaagaat ggtgggacag catcgactct tggagaaaag tatacagcac
     8521 atggtgtaaa gcctacccag agcagcgtga agaagtcttt caattgtttg aaaaaaagga
     8581 agatttttgg ctacgtcgaa tagcaatcac gttgcaatta ggttataaac agcaaacaga
     8641 cacaggacta ttgatccgag caatagacga agatcgtcaa acaaaggaat tctttatcca
     8701 aaaagcaatt ggttgggcat tacgcgatta cagtaagacc gatgccgatt gggtaaaaag
     8761 acagctgaca acagatttga gtaagttagc tgttcgagag gcaagtaagt atttataaga
     8821 aagcgagacg acaagagatg agacgcaaat tatttgcttt tgatattgat ggaacgttgt
     8881 tagattcaaa caaacaagct ttagatagca cgagagaagc attggaaaaa ttacgcaaag
     8941 ccggtcatct ggtcacgatt gctacagggc ggagccgatt tcatgcgcaa gagatcattc
     9001 gcgatcttga ttttacgaac tacattttat gtaatggagc ggccggtttt ttagaccatg
     9061 aacaagtata taagaattta ctagatcgcg accagttgga tgcgttcgtt caagaagcgc
     9121 atcatgagca gatcgataca gcttttgtca gcttagacaa tatcaagcgt tcttcttcca
     9181 atgatgtaac ggtgatggaa gaagcgatgc attctttcgg tgctgtcttg cctgaattag
     9241 atcagttttt tgcagaggaa caagaaatat accaagcgtt agcatttttc gatcaaacat
     9301 atgatgggaa gttctcttca tatgataaaa ttcgatttgt ccgctggcat gagaacagtg
     9361 tagatgtcgt acctaaaaat ggttctaaag cagcgacaat tctgcactta gcagaacaag
     9421 taggcatccc tcatgaagac atcatcagct ttggcgatgg acaaaacgac cgtgaaatgt
     9481 tgcgcatgtc aggaattggg atagctatgg ggaatgctgt gccagaagta caagcagaag
     9541 ctaagatggt cactgataca aacgatcagg atggaatttg gaaggcattg aaagagttaa
     9601 gcgtgattta aaagaggttg tgaaaaagct gttctgcatc aagtaataag cccagccaaa
     9661 caaaaatagc tcctcctatt tttcgttttt ctgggcttat taccgcagat gcaagctttt
     9721 gaacaccgtt taaggaagag gtcgtgacag aagtggtcag tttcaagaaa taagaaggga
     9781 tttacgaaaa ttgcttctca aatttttgta aaattacggc ttatttctga agaagtagat
     9841 gaaacaacgt ttattcggga aatgggactg tgacaagatg tttgtcatag tcccattttt
     9901 ttattttatt tttcaaaact aagaaatacc aggaaagagt tccttttgtt tgaccttttc
     9961 tttcttattg aaaacaacta taatatagag tgatgttaaa agagaagagg aatttagtgt
    10021 gcgggtattg tttattggtg atgtagtagg ttcaatggga cgagagatga ttactgaata
    10081 tcttcctaga ttgaagaaga agtaccgtcc gcaagtaacg attgtaaatg gtgagaatgc
    10141 cgcttctgga cgtggaatca cagaaaaaat ctataaaaaa tttttgcaag acggcgtaga
    10201 tgtcgtgacg atggggaatc atacgtggga caaccgcgga atctttgagt ttgtaaatga
    10261 agctaaaaag atggttcgtc cggcaaattt tccggaaggg acgcctggac aagggatggt
    10321 ctttgtcaaa gtgaaccaag tcgaactggc agtgatcaac atgcaggctc gttcttttat
    10381 ggtggacttg gatgatcctt ttcgtaaaat gaatgagctg gtagaagaag ccagaaaacg
    10441 tacgccgatc atttttgtag attttcatgg agaaacaaca agtgaaaaac aagcaatggg
    10501 ctggttttta gatgggaaag tttcagcagt ggtcggtaca catacacacg ttcagacaaa
    10561 tgatgcgcga attttgccaa gaggaacagc gtatctgaca gacgttggga tgacaggtcc
    10621 ctatgatggt attttgggta tgcgcagaga acctgtaatc gaaaagtttc tgaccgcatt
    10681 accaaaacgt tttgaagttg tggaacaagg ccgatcgatt ttaagcggct gtatcttgga
    10741 attggatgat acgacaggtc atgcaaaaaa aatccagctg attcagatca acgatgatcg
    10801 accgttcatg gagtagaaag agaggcagtt ttttgcagca ggagccggaa attcaaaaag
    10861 aagtaaggaa actgacaaaa cttttaagag agaatgaaac gatcattcgc tacaaagaac
    10921 ttgaagaaaa gatccagcaa aaccagtatt tagctgagtt gagagaaaaa atcaaacaag
    10981 cgcaaaaaga tgctgttcat tttgcccact atgacaaacc agcagcagaa caagaagcaa
    11041 tcaaacaagc ggatcaattc atgcaagaat ttgatcagca tccgcttgtt gtagcttata
    11101 gaaaacagct gttggaagca gatgatctgc ttcatcattt aacaactatg atacaagaag
    11161 aaatcaatgg acaaatagag gaggaaaaac atgcctcaaa aaactaaaaa tactcctatg
    11221 atggaacaat atctaagcat caaggcccaa taccaagatg cttttttgtt ctatcgttta
    11281 ggagactttt atgaactatt ttacgaagat gcgatcaaag cgtcacagct tttggaatta
    11341 acgttgacga gcagaaatcg aaatgcagaa gaacctattc ctatgtgcgg tgtacctcac
    11401 catgcagcac aaggatatat cgatgccttg attgaaaaag gctataaagt agcgatttgc
    11461 gagcaggtag aagatccaaa gacgactaaa gggatggtca agcgcgaagt gatccagcta
    11521 gtcactcctg gtacagtcat ggacagcaaa ggattgtcgg caaaagacaa caatttttta
    11581 acagctgtag ttagtcaagg aaatgaattt ggctttgctt atgccgattt aagtacggga
    11641 gaattgaaga cggctcggtt gcatgatgaa gaagctgttt taaatgaagc ctctgcattg
    11701 cagacaaaag aactcgtatt aggcagtgag atcactgata cccttcgtga acagctaagc
    11761 gggcgtttag ggctagtctt ttctcaacaa aatgagatgg aagaaaacgc ggaatttagc
    11821 tttttgacaa gcgaactagt cgatccgctg aaaaaagaag caaccggcaa actattgact
    11881 tatttatcgg tgacacaaaa gcggagttta gctcatatcc aaaaagcagt agaataccaa
    11941 ccagatcatt ttttgaaaat ggattattat tcaaaattca acctagaatt aagccaatct
    12001 attcggactg ggaaaaagca cggcactctt ctatggttgt tagatgaaac aaaaacagca
    12061 atgggtggcc gtttactaaa acaatggcta gaccgtccgc ttatccaggc aaaacaaatt
    12121 aaagcaagac aagaaatggt cgcttctttg ttagacagct attttgaacg tatcgatttg
    12181 cagtcagcat taacgaaagt ctatgatctg gaacgcctag caggtcgagt agctttcggt
    12241 aatgtcaatg ggcgagactt gatccagctg aaaaattctt tagaacaagt gccaatgatc
    12301 agagggttga ttgaaggaat cgatcaaggt caatggcaaa gtctgctttc agaaatgcag
    12361 ccgatggacc atctcgtcca attgatcgac caagctatcc aagatgatcc gccgttacaa
    12421 ataacggaag gaaacatcat caaagatgga ttcaatgagc agctcgatat gtaccgttct
    12481 gctatgagaa acggaaagaa atggttggca gaacttgaag caaaagagcg tcaagaaaca
    12541 ggaatcaaaa acttaaaaat cggatttaac cgcgttttcg gttattatat agaaatcaca
    12601 aaagcaaatc ttggaaatgc agaactggag aagtatgaac gaaaacaaac tttagcaaat
    12661 gcagagcgtt tcatcacacc agaactaaaa gaattggaga cacaaatcct cgaagcagaa
    12721 gaaaaatctg ttgatttaga atatcagttg tttttagctg tgagagaaga agtcaaaaag
    12781 gctatccagc cgctacaagt acttgcaaaa gcaatcagtg cagtagatgt gctacaaagt
    12841 tttgcaacga tcagcgaacg ctatcagtat gttcggccag aacttgtcag tgacaagcat
    12901 cagcttctga tcaaagacgg acgacatcct gtagtagaaa aagtattagg acatcaggaa
    12961 tatatcccta acagcgtaga aatggcagaa gacgaaatga tcttgctgat tacaggaccg
    13021 aatatgtctg gtaaaagtac gtatatgcga cagttggcat taactgttct gatggcgcaa
    13081 atgggctgtt tcgttccggc aaaacaagca gttttaccta tttttgatcg gatttttacc
    13141 cgaattggtg caagtgacga cttaattgct ggacaaagta cttttatggt agagatgatg
    13201 gaagccaatc aagccctgcg ttatgctacg ccaaacagtt tgattttgtt tgacgaacta
    13261 gggcgaggaa cagcaaccta tgacggaatg gcgttggccc aagcaatcat cgaatatatc
    13321 catcgtcatg tacaagcaaa aacactcttt tctactcatt atcatgaact gacggtactt
    13381 gagaaagaac ttccgcagct taaaaatgtc catgttggag ccgttgaaaa agacggagaa
    13441 gttgtcttct tgcataaaat gatggatgga cctgcggata aaagctacgg catacatgtg
    13501 gcaaagatcg caggattgcc ttccaatcta ctagaacgag cagcagatat cctgcatacg
    13561 ctagaaaatg gcgataacgg acaccatact gttccagctg aagtagaaga gaaaactaaa
    13621 acagccaaac aggagcaaat aaaaccacct gcagaaaaag taaatcaaga ccatatacaa
    13681 gaccgtgtaa aagaagaaaa ccaacagctt tctttattca gtgaattatc cgaaaatgaa
    13741 acagaagtca ttgaatcact gaagcaaata aatttactag aaatgacgcc aatggatgca
    13801 ctaaacacat tatatgaatt acaaaaacaa ctgtaggaag aaggcggata tcgatggcca
    13861 aaatccaaga attatctgaa cgtttagcta atcagatcgc tgcgggagaa gtagtggagc
    13921 gaccagcttc cgttgtgaaa gaattagtgg aaaatgcaat tgacgccggc agtactcaga
    13981 ttgatatctt agttgaagaa gcgggactgc ggacgatcca agtgattgat aacggagaag
    14041 gcattttggc agacgacgta gagaatgcct tcaagcggca tgcgacaagt aagatccata
    14101 atcgcgacga tctttttcgc atacgaacgc tgggctttcg aggggaagct ttgccaagta
    14161 ttgcctctgt ttctgaaatg actctggaaa cggcgaatca ggaagaaaaa caaggcacct
    14221 atctgtcgct gaaaggcgga gaagttattg agcatcgacc agcaccactg cgtcaaggaa
    14281 cgaagattac cgtcagcaat ctgtttttca atacgccagc ccgcttgaaa tacgtgaaaa
    14341 cactgcacac agaattagcg aacatcgggg acatcgtcaa tcgactggca ttgagtcatc
    14401 catcgattgc ttttcgttta gtacatgatg gaaacaaaat gctggcaact gctggaaatg
    14461 gcgatctgaa acaaacaatt gcgggaatct acggattatc taccgcaaaa aaaatgctga
    14521 aaatcgaagg aaaagatcta gattttgagt tatttggcta tgtttcattg cctgaggtga
    14581 ctcgtgccag taggaattac ttatcgacga tcatcaatgg acgttttatc aagaactttt
    14641 ccttgaataa agcaatcgtt gcaggttatg ggtcaaaatt aatggtcggt cgtttcccta
    14701 ttgctgtatt ggaaatcaaa atggacccgc ttttagtaga tgtaaatgtt catccgacga
    14761 agcaggaagt ccggctgtcg aaggaaaaag aactaacaga gctaattagt aacgccattc
    14821 aaaaagcatt gtctcaagaa aatctgattc caagtgcagc ggataatcta cggtttaaga
    14881 aaaaagtacc tgatcagcct aaaacagaac aaatcgaaat cgatttgtcc tacgcttttg
    14941 aagaagaaaa acctaaaaag ccaagtagtt tgaattttga tcgagagaca ggtacctttt
    15001 tcgtagaaga accaaagaac acatttgttg acagaattgt tgataagtct gtggaaaatg
    15061 ttgaaaacta ttctggtttt tctgaagaaa acaccactta tccagaaaaa acagcgaaaa
    15121 cggtggataa gtttggtgaa aatgttgata actttgtaga aaaaacagtg gaaaaatctg
    15181 aaaagaaaga atttcccgtt gtggataaca tggagaaaac aaatgaagaa caagcagata
    15241 aatccgtaga agaggatgaa aagatttacg aggaagaatt gacactccat cctgaatttg
    15301 atttcactca aggaaacgac aaaacattag aacaagccat caagaaatta gaaacggaaa
    15361 aaccagtaca acgttttcct cagttggaat attttggaca gatgcacgga acgtatcttt
    15421 ttgcccaaag tagcgatggg ttgtacatca ttgatcagca tgctgcccaa gagcgcatta
    15481 agtatgaata tttccgggaa aaaattgggg aagtatctga tgatctgcaa gaattgcttg
    15541 ttccatttgt gttagactat ccaaacagtg atgctttgaa attaaaagag caaaaacact
    15601 tgctagaaga tgcagggatt tatttagaag aatttggtca aaacagcttt attgtacggg
    15661 cccatcctac ttggtatccc aatggagaag aagaatcgat tattcgggag atgatcgata
    15721 tgctgctgac aactggaagt gtgagtgtaa aaaaattccg tgaagcaaca gcaatcatga
    15781 tgagttgcaa acgatcgatt aaagccaacc attatttgaa tgaagcacaa gcacgtgtgc
    15841 tgctgaagga tctggcacga tgcgaaaatc ctttcaattg tccccatggc cgtccagtat
    15901 tgattcactt taccaattcg gatatggaaa ggatgttcaa acgaatccaa gacccgcatt
    15961 aagtcaaaga gaagagggaa aacatgaaga ttgtattagc atcccaatca ccaagaagaa
    16021 aagagttatt ggctcatatc gtttcagaat tcgagattga acctgctgat atcgatgaaa
    16081 cgccatttcc agacgaacag cccgtcgatt atgttcgtag aatggctgaa gaaaaagcac
    16141 gaattgtctg ggaacaagat gaaaaagaag accatttagt gattgccagt gacacgactg
    16201 ttgttctcaa tcaagagatc atgggtaaac cagaagatct ggctgaggct gaattcatgc
    16261 tgaaaaaact aagcggggaa acacatactg tatatacagc tgttgtttta aaaacaaagg
    16321 aacgagaaga acggatacta gcagaagcac atgtcacttt ttatcctttg acagacgaag
    16381 agatcaaacg ctacttggag acgggggatt atgcggataa ggcaggggca tacggcatcc
    16441 aaaatgctgc gagtgtattc gtcaaagaaa tttctggaga ttattacagt atcgtaggat
    16501 ttccgattgg tgctgtcaat caggcactga aaacattcat ttaaacaaaa aagcggacta
    16561 tctgattttc gattgaaaat ccatgatagc ccgttttttt tattgaaaag attacttatc
    16621 tactctgact aa
//
LOCUS       DAXWOO010000051        16143 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.051, whole genome shotgun sequence.
ACCESSION   DAXWOO010000051 DAXWOO010000000
VERSION     DAXWOO010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.051"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..851
                     /locus_tag="ACGE70_001306"
     CDS             <1..851
                     /locus_tag="ACGE70_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFF8692953.1"
                     /translation="LKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVETVAE
                     TYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRVAVV
                     GITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRSTFEK
                     WVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTDENV
                     TTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVERDWQ
                     GNIVKE"
     gene            966..1886
                     /gene="pstC"
                     /locus_tag="ACGE70_001307"
     CDS             966..1886
                     /gene="pstC"
                     /locus_tag="ACGE70_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFF8692954.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTITTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            1886..2770
                     /gene="pstA"
                     /locus_tag="ACGE70_001308"
     CDS             1886..2770
                     /gene="pstA"
                     /locus_tag="ACGE70_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFF8692955.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            2785..3591
                     /gene="pstB"
                     /locus_tag="ACGE70_001309"
     CDS             2785..3591
                     /gene="pstB"
                     /locus_tag="ACGE70_001309"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFF8692956.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            3607..4365
                     /gene="pstB"
                     /locus_tag="ACGE70_001310"
     CDS             3607..4365
                     /gene="pstB"
                     /locus_tag="ACGE70_001310"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFF8692957.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            4378..5055
                     /gene="phoU"
                     /locus_tag="ACGE70_001311"
     CDS             4378..5055
                     /gene="phoU"
                     /locus_tag="ACGE70_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFF8692958.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            5632..6324
                     /locus_tag="ACGE70_001312"
     CDS             5632..6324
                     /locus_tag="ACGE70_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFF8692959.1"
                     /translation="MLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISIL
                     FVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYL
                     TRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVI
                     GSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPIS
                     VLFFLTVRRLKR"
     gene            6321..7088
                     /locus_tag="ACGE70_001313"
     CDS             6321..7088
                     /locus_tag="ACGE70_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFF8692960.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            7188..8756
                     /gene="liaX"
                     /locus_tag="ACGE70_001314"
     CDS             7188..8756
                     /gene="liaX"
                     /locus_tag="ACGE70_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFF8692961.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            9053..9373
                     /locus_tag="ACGE70_001315"
     CDS             9053..9373
                     /locus_tag="ACGE70_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFF8692962.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            9373..9723
                     /locus_tag="ACGE70_001316"
     CDS             9373..9723
                     /locus_tag="ACGE70_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFF8692963.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            9890..10828
                     /gene="hprK"
                     /locus_tag="ACGE70_001317"
     CDS             9890..10828
                     /gene="hprK"
                     /locus_tag="ACGE70_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFF8692964.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANKNGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            10843..11673
                     /gene="lgt"
                     /locus_tag="ACGE70_001318"
     CDS             10843..11673
                     /gene="lgt"
                     /locus_tag="ACGE70_001318"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFF8692965.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            11717..12742
                     /locus_tag="ACGE70_001319"
     CDS             11717..12742
                     /locus_tag="ACGE70_001319"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFF8692966.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSRAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            12757..13695
                     /gene="galU"
                     /locus_tag="ACGE70_001320"
     CDS             12757..13695
                     /gene="galU"
                     /locus_tag="ACGE70_001320"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFF8692967.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            13820..14260
                     /locus_tag="ACGE70_001321"
     CDS             13820..14260
                     /locus_tag="ACGE70_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFF8692968.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            14263..14865
                     /locus_tag="ACGE70_001322"
     CDS             14263..14865
                     /locus_tag="ACGE70_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFF8692969.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(14924..16030)
                     /locus_tag="ACGE70_001323"
     CDS             complement(14924..16030)
                     /locus_tag="ACGE70_001323"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFF8692970.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
BASE COUNT         5232 a         2819 c         3491 g         4601 t
ORIGIN      
        1 aacttaaatt attcttacca ataataggat tagctattct tttatcaggc tgttcaaagt
       61 ggattgatcg aggagaatcc attacggctg ttggctcttc tgctttacag cctttagtag
      121 aaacagttgc tgaaacttac cagacagaaa atcctggaaa attcatcaat gtacaagggg
      181 gcggaagcgg aactggtttg tctcaagtac agtcaggagc agtcgacatc ggaaactctg
      241 atttgtttgc ggaagaaaaa tcggggatcg atgcctcagc attagtcgat catcgagtag
      301 cagttgttgg gatcactcct attgtgaaca aggatattgg cgtaaaagac atcagtatgg
      361 aaaacctgaa aaaactattt acaggcgaga tcaaaaactg gagtgaagtc ggtggaaaag
      421 atcagccagt agttatattg aaccgcgctt caggaagcgg aacccgtagc acgtttgaaa
      481 aatgggtatt ggacggagaa acagctgttc aggcacaaga acaagattca agcggaatgg
      541 tgcgccagat cgttgccgac acacccggtg caatcagcta tgttgctttt tcttatgttt
      601 ccgatgatgt agatacgttg aatgttaacg gtgtagaacc gacagatgaa aatgtgacaa
      661 cgaacaaatg gacgatttgg gcatatgagc atatgtacac aagagatcat ccaaaaaaac
      721 tgacaaaaga atttttagac tatatcttat ctgatgaagt acaagataac atcgttggag
      781 aactaggtta tatccctgtt tctcatatga aagtcgaacg tgattggcaa ggaaatatcg
      841 ttaaagagta atctttacac aaaatttaca aacttagcta aacttttaca tgttattaaa
      901 accaaattga tgttcaattt gtacactgag tgagtatgtt aaacctgatt aagggggagt
      961 catctgtgga agatgtgaaa aaagtattat taacaaaatc aaaacgttca aaaatggaac
     1021 agcgtggaaa attcatcagt tttttatgta tcgcgctgat cgtgttggtt gtattatcta
     1081 ttttttattt tgtagctagt aaaggattgg ccaccttttt tactgataag atcaatgtat
     1141 ttgattttct gtttggtaca gattggaatc caagccaaat cggagcaaat gggaaaccaa
     1201 tggtaggggc attgcctatg atcacaggtt cgtttattgt gaccttttta tctgctatcg
     1261 ttgccacgcc atttgccatt ggtgcagcag tattcatgac ggagatctct cctaaaaacg
     1321 gaacgaagat cttacagccg gtcatcgagc tgcttgtagg tattccttct gtcgtttatg
     1381 gttttatcgg tttatcagtt atcgtgcctt ttgtcagaag tatttttggc gggactgggt
     1441 ttggtatctt agctggtaca tttgtgttgt tcgttatgat cttgccgact atcacaacga
     1501 tgactgttga tgcattaaag gcagtgccac ggcactatcg ggaagcttca ttagcgcttg
     1561 gtgccacaag atggcaaaca atctacaaag tcgtgctacg agcagccgtg cccgggattt
     1621 taacagcagt cgtttttggt atggctcgtg cgtttggtga agccttagct atccaaatgg
     1681 tcatcggtaa tgcagcattg atgccaacag gtcttactac tcctgcttct acattgacgt
     1741 ccatcttaac aatgggaatc ggaaatacaa tcatgggaac agttgaaaat aatgtgttat
     1801 ggtcattagc gctgatcttg ctattgatgt cattattctt taatatcgtg atccgtatga
     1861 ttgggaagaa aggagagctg aaataatgaa cgcaaaacgc tcagataaaa ttgctacagg
     1921 tgttttatat atagtttcgg ggatcatcgt attgatttta gcagctttac tactttatat
     1981 cttagttcgg ggaataccgc acatttcatg gtccttcttg accgaacctt ccaaagccta
     2041 ccaagaaggc ggtggaatcg ggatccagct atttaactcg atttatttgc ttttgatcac
     2101 gatgatcatc agttttccta tttctttagg tgcgggtatc tatctttcag aatatgctgg
     2161 aaaaaactgg ctaacagatg tgattcgcac gtcgattgaa atcttgagct cgttgccttc
     2221 tgtcgttgtc ggtttattcg gtttcttggt tttcgttatt caattccagt atggtttctc
     2281 gattatttct ggtgcattgg ctttgactgt attcaacttg cctcttttga ctagaacagt
     2341 tgaggagtct ttacaagcgg tccattacac acaacgagaa gcaggattgg ccttaggttt
     2401 gtcacgctgg gaaacagtga taaaagtggt ggttccagaa gcgacaccag ggattttgac
     2461 tggtgtgatc ctaagttctg gaagaatttt tggtgaagca gctgcattga tctatacggc
     2521 tggacaaagt gcacctgcgt tagattttac gaactggaat ccattaagtg tttcaagccc
     2581 gatcagcatt ttccgtcaag ctgaaacatt agctgtccat atttggaaga tcaatacaga
     2641 aggcacgatg cctgatggtg tttctgtttc ggctggcgct tctgccgtgt tgattattgc
     2701 tgttttactg ttcaacttcg gcgctcgtgc gattggcaaa cgattatacc gaaaaatgac
     2761 ttcagcctaa ggagaagaag caaaatgaaa aaatataact ggaatgaaac aaatgtcatc
     2821 accatgccaa aagagaaaga agtagcacta tatacggatg atctacatgt ctggtatggc
     2881 gacaatgaag caatcaaagg agtggatctg gaatttgaaa aaaataagat cactgcactg
     2941 attggtcctt ccggctgcgg aaaatctacg tatcttcgtt cgttgaatcg aatgaatgat
     3001 gggatcgcta atacgaaaat cactgggaaa atcatgtatc aaggcgtaga tattaatgcg
     3061 cctcaagtgg atgtttatga gatgcgcaaa cggattggca tggtttttca acgtcctaat
     3121 ccgttcagca agtcgattta tgacaatatt acatttgctt tgaaacagca cggcgaaaaa
     3181 aacaaaaaga aattagatga aatcgttgaa acaagcttga aacaagctgc cttgtgggac
     3241 caagtaaaag acagcttgga caaaagtgcc ttggctttat ctggcgggca acagcaacgt
     3301 ttatgtattg ctcgtgcgat cgcgatgaaa cctgatatct tgctgctaga cgagccggca
     3361 agtgctttgg accctatttc gacaggaacg gttgaagaaa cacttgtaaa cttgaaagaa
     3421 gactatacga tcatcatcgt gacgcataac atgcaacaag cagctcgtat cagcgactat
     3481 actgcctttt tctatttagg aaaagtaatg gaatatgaca aaactaacaa gatatttacc
     3541 agaccaaaaa ttcaggcaac cgaagattat gtgtccggtc attttggtta agacggaggc
     3601 ctcaacatga caaaagagat tatttcatct aaagatcttc atctgtatta tggaaagaaa
     3661 gaagcactaa aagggatcga tctaagcttc aatcaagggg aaatcactgc gatgatcggt
     3721 ccttctggtt gcggaaaatc cacttatctg cgttctttga accggatgaa tgacctgatt
     3781 ccgaatgtaa cgatcaccgg aagtgtttta tataaagaca aagacatcta tggtccaaaa
     3841 acagatgttg ttgaactaag aaaagaaatc ggcatggtat tccagcagcc aaacccattt
     3901 cctttttcta tctatgaaaa tgtcatctat ggattgaaat taaaagggga aaaagataaa
     3961 aaagtccttg atcaagtagt tgaagaaagc ctgaaggcag cgtctgtttg ggatgatgtg
     4021 aaagacaaat tgcataaaag tgctttatct ttatccggtg ggcagcagca gcgtgtatgt
     4081 atcgcacgtg ttttggcagt taatccggaa atcatcttat tggatgaacc aacaagtgcg
     4141 cttgatcctg tttctactgg gaaaatcgaa agtatgctgt tagaattaag agagcggtat
     4201 acgatgatca tcgtgaccca taatatgtct caagcttcac gtatttcaga taagacggca
     4261 ttctttttag atggtcactt gatcgaatac aatgatacga agaagatatt catgaatcca
     4321 gaaaaacaag agacagaaga ttatatttca ggtcgatttg gataaggagg aaacggaatg
     4381 ctacgtacac aatttgaaga agaattgctg aatctccata atcagttcta cgaaatgggg
     4441 atgatggtga gcagcgccgt gcacaagtca gttcgtgcat acatcaaaca cgataaaatc
     4501 ttagcccaag aagttatcga aaacgatata aatatcaata acatggaaac acgcttggag
     4561 aagaaaagtt ttgaaatgat cgcattgcaa cagccagtta caactgattt gcgtatgatc
     4621 attacggtca tgaaagctag ctcggattta gaacgtatgg ccgatcatgc ggtgtctgtt
     4681 gctaaatcca cgattcgcgt aaaagggcag acgagaatac cggaaatcga aaaagagatc
     4741 tccgatatgt ccgattatgt gaaaaaaatg gtggacaacg tcctagttgc ttatgtcaaa
     4801 acagacgaaa aagacgcacg tacgatcgcc aatatggatc aaagagtcaa tgactatttc
     4861 aatcgaatct ataatgcaac aatcaaaaat atgcaggaaa atcctgagac agtcatcagt
     4921 ggaacagact acttgaatgt tgccagttat ctagaacgga tcggagacta tgtgacaaac
     4981 atctgtgaat ggatcgtcta cttggctaca gggaaaatca ctgagttaaa tacgaaccac
     5041 aatgagattt tctaaactga agctgtgatt atgccgtttg tttaggaaaa gaggttgtga
     5101 aatcagcggt tgtgaaaaag ctgttctgca tcaagtaata agaacagtca aacaaaaaat
     5161 agctcctcat atttttcgtt tggctggact tattaccgca gatgcaagct tttgaacacc
     5221 gtttattcgg gaaagggttt gtgacaaaac atgtcacaaa cccttttcat catttcatag
     5281 aaaggataaa gaatgcaaaa aaaaattagc atcatcacta tattttttat ttgtgtgctt
     5341 acttcatggg ttattcagca tcaagccgct ttatatgaag tacctgtcat cgagatagaa
     5401 aaagtacgaa taaaagagga tcatcaattg attacaggaa aactgatgaa cacggatcaa
     5461 aaaggagaat cgatttattt atcggttcct tatcaaaaaa atcaaataga aacagcaaat
     5521 ataaaagcga gacaaatgta tctagtccaa atgcatggag atgaatggca gctagtagca
     5581 aaaaaaaatg atggctggct tttcttcttc acaagtatct tcattggcgc catgttgctt
     5641 attggtggaa aacagggtct atttgcttta ggaagtctca tcatcaatat cgttttactt
     5701 ttacttttgt tgctcatctt tactaaaaca gaagggattt ctctattaaa tatatctatc
     5761 ctgttcgtcg ttatagggat gattattagc atattggctt tagatggatg gaatagaagc
     5821 agtataatca aaatttcagc tgcagttacg agtgtttttt tagcattttt tatttgtctt
     5881 ttgacaatga atcattggaa agaccaagga ctgcgctatg aggaactgaa ttatctgaca
     5941 cgaccgtatc gttctgtttt tctttctagc gtactgattg gaacagctgg aggaacactc
     6001 gataccgtta tcacagtcat tgcgacatta gaagaattga ctaagcgaca accagaaata
     6061 acagcgaaac agttatggct ttcaggaagg caagtgggtc gagatgtgat tggctcgatg
     6121 accaatatcc ttttgttcgt ctatatcagt gggtcagttc catcgcttct tttatattta
     6181 ggaaatcaat ggtcgttcaa agaaacgata gaacagcatt tatcattgga atttttacgc
     6241 gtcattgctg gcagcttagg aatagtatta tcgatcccta tctcggtcct cttttttctg
     6301 actgtaagga ggctgaaacg atgaatgtac ttattatact tgttgggatt ttagcaatca
     6361 gcgtgctact tattggcggt acgcaaggta tgtatattct tttaggctta ttcatcaatt
     6421 tagggatctt tttcctgttg ttattcggtt atcatcagaa gtggccgata ctggcattgt
     6481 cggtcatagg atttttattg attgctgtgg ttatcttatt ttttatcaac ggttataatc
     6541 taaaaatgcg tgcagctttt gtgagtatac tgatcttttt attttgtttc ctactattgc
     6601 ttcctatcac tgatttttta gcaatccaag gttttacatc ggtagaacta gaagaattat
     6661 ctggcttaga taagacttta gcgattgatt ttagattact tacgagatcg ttgcttctga
     6721 ttagtcttag tggtgctgtt ttagatgcga gtgtagcaat ttctagtggg acattcgaag
     6781 tatatcaggc aaatccgcaa ttatcttttt accaattgcg ccattctagt ttcgctattg
     6841 ctaaaaaaat attggcgtca acagtaatga cacttctttt tgcattcatg ggaagttctt
     6901 tagcgttgat cctctggttt attgatttag acattccttt tcagcaagtg atcaatgaaa
     6961 aatcatttgt tctagaatat acgatggcta ttcttaccac attgtccgca ctacttgttc
     7021 ttcctttaac ctgcgtgact acagcatatc tctttacaaa aaagaaagaa caactcaaga
     7081 tggagtaaaa gttacaactt aagtcctatg acatttttac tgaatcagtg cataatatgg
     7141 atgtaaggaa aagcttgcaa acaattttgc ttaaggaggc aatttccatg aaagaaagag
     7201 aacgtatttt agatttagtg aaaaaaggaa tcctttcgac tgaagaaggc ttagatttat
     7261 tagaaagcat ggcaactgaa aaagacgaaa agcaaatcaa aaaagaagca gacaaggtaa
     7321 ctgctagtca taaagaaaaa gatcaggcaa gtcaattgat cgataaactt gaaaacggcg
     7381 aagaagaaat ttctgagcca gttgatccaa aggaaagaga aagacaagat caagaaaact
     7441 tagaaaagat cttagatgaa ctagcaactg aagcgaataa aacttcggcg cgtctagatg
     7501 aagtaaatgc tcaattcgct gacaataagg cagcacgcaa tgagaaacag gaagcattga
     7561 tgcagctgaa tacaaaagaa gaattaggcg agttgacaga agaagaatta gcacagcgtc
     7621 agacgttaga agcagaaatc aaagaattag aagctagtgg cgatacactg ttagaagaac
     7681 aattgaaatt agaagcagaa ttaaaagata tccgtaagaa tcaatggtct gagaaaaaag
     7741 aaacgtttac tgataagttt gaattgccag atgattggaa agatcaagca acagatacac
     7801 tgaatcaagt aggcgaaaaa atgtcagaag ctggatcaca gttaggcaaa ttcttgaaga
     7861 aaacattcca aactgtttca gaaacagtga atgacaacat ggaatggaaa gatgtcagct
     7921 tacgtgtccc tgggattgcg acaacgaaat ttgagcatga attttactat gaagctcctg
     7981 cagcaagtat ccttgatatc aaggcagcaa acggaaacgt gacgttaaaa acgtgggaca
     8041 gcaacgatgt gaaagttgaa gctaagatca aactttacgg aaaaatgggt gctgaaccat
     8101 ttgaagcatt ctctgaacgg agccagattg aggttaatga agatcatatc tccttccaaa
     8161 ttccaaataa acgtgtgcgt gcagaccttg ttttctactt acctaaacga gtatacgacc
     8221 acgcagctat taaacttcta aatggcaaca tcatgattga aacattggaa gctaaagata
     8281 tttatacaaa atctacgaat ggtaacatca tcgtggacca attaactgca acgatgctgg
     8341 aagtagaagg tgtaaacggc aatatcgata ttcgaaacgg gaacatctta gattctatta
     8401 tcgaaacagt aaatggtaca gtgacatttg gtgcgacagc agaaaacctt tctgtgtctt
     8461 tggtaaatgg tgatgtacgt cttacgatta aagaagataa tttgaaaaaa gtagaagcaa
     8521 gttcagtgaa cggaaatgtc aaagtcgctt taccagatgg catcggtttg gaaggacatg
     8581 ctaaaacaag tcttggaagt atcaacagcc gtctgtccaa ttacgaagta gttcgtgaga
     8641 aaaaagaacg gacaaaccaa atgttgcaat tccgtcgtgt ttcagatggt gaaatcgcgc
     8701 aagtgcaact ttcaacgaca acaggaagta tctatttaaa agatacagat aaataaagag
     8761 gttgtgaaag agatgttcag ccttgagcaa taagaagcga ttttgtaaaa tagcttcccc
     8821 tattttacaa aattgtgcct tattgccgat agtctatctc ttgaacaccg tttaccaaga
     8881 gattatgaac ctgccaatta tcgccgagtc acaaggaata atttcagaaa aagctcgtca
     8941 cgttttatga atatggtaat ttgtgaccga gaattttgca gaaagacacc gattaagaag
     9001 aaggttgtca caacgatttt cttcatttaa aatagtagaa cagaggtgag acatgatgaa
     9061 aaaaaagttg acaaaatcta ataaaaatgt tgttttgaca gggactttag caggaatagg
     9121 agaatacatc gggattgatc cgactgttat ccgtgtgctt tatgtattcc tgagcttagt
     9181 tgcttttgga agtccaatta ttttatacat tttattagct ttgattatcc catctgcgcg
     9241 ttctgcacga acaaactatg gacataacaa tgcatattat gaaaaaaaca attatcgtga
     9301 acaaactcgt gaacaaacaa aaaaacgcaa agaagcggaa aaaatcgatg atgacgaatg
     9361 gagtgacttt taatgtcgta ctttcaacgt attttagtta atacgttaac attcatatca
     9421 ttggctgttc tttttcctgc gatggtccat gtgagcagta tttggacggc cgttcttgcc
     9481 agcttcgtat tgtctgtgtt gaatatgttt gtcaaaccga ttttgacgat actgtccctg
     9541 ccctttacat tagtcacatt gggattgttt acgtttgtta tcaatgcagc gattcttaaa
     9601 atgacctcct tttttgtggg agcagaacat tttagttttt cttcattcgg tgcagcgttg
     9661 ttagtagcta tcttgatgtc gcttgtcaat gtaattgtca gcgaacataa cagtagaaaa
     9721 taaaagaaag ctgtgccgaa ctatcggcgc agttttttta tttagaatca ggaatgcgtt
     9781 ttctattaca ttggtaatta aataaaaacg ttttttttaa ttggtagtac ttttggctag
     9841 acaatgataa aatgatagag aacactctaa tagaaatgag gtagcatgaa tgtcagaagt
     9901 tgtaaaagtt agccaattag tgaaaaaatt atctttggaa atcgtgacag gcgacgagaa
     9961 aagcttagac cgtgtcatcg taactgggga catctcacgc ccaggactag agttgactgg
    10021 ttattttaat tattattccc ataatcgttt acagctattt ggcagtaaag aaatcagttt
    10081 tgcggaacgg atgatcccag aagagcgttt gatcatcatg agaagattat gtagtgaaga
    10141 tatgcccgca tttattattt caagaggatt accagctcca gaagaaatga tccaggcagc
    10201 aaataaaaat gggatagctg tactgcgttc tcccatctca acttctcgtt tattaggaga
    10261 attatccagt tatttagatg gtcgccttgc ccctcgaaca agtgtccacg gtgttttagt
    10321 ggatgtttac ggattaggtg tactgattca aggtgacagt gggataggaa aaagtgaaac
    10381 agcgttggag ttgatcaaac gcggccatcg attgatcgca gacgatcgag tagatgttta
    10441 tcagcaagat gaattaacgg tgatcggaga accgcctaaa atcctggaac atctgattga
    10501 aatccgagga atcgggatca ttgatgtaat gaatcttttt ggtgcgagtg cggtacgtgg
    10561 ctctatgcaa gtccagttgg ctgtttatct ggaagcatgg gcaaaagata agaaatacga
    10621 ccgtctgggc tctgaagaca cgaccgttga aatcgcggaa gtgggcattc cacaaataaa
    10681 aatccctgtc aaaacaggac gaaacgttgc aatcatcatt gaagtagccg cgatgaattt
    10741 ccgtgcacgt acaatgggct ttgacgcaac gaaaacattt gaagaaagat taagtcgctt
    10801 gattgaagaa aactcaggca acgactagca aggagtgaca atatgttagc acaaatcaat
    10861 cgagttgctt ttgagcttgt agggattcct atctactggt atgcattgat catcgtatca
    10921 ggtatcatta tcgctatgtg gcttagcagc agagaggctg ttcgggtagg attaaaagca
    10981 gatgatgtga cagattttat gttgtgggga ttgcctatct ccattattgg cgctcgctta
    11041 tattatgttt tatttgatct tccccagtat atagctgatc caatccaaat attcaatatc
    11101 cgttcaggag gcttagctat ttatggcgga ctgattgcag gcgcgatcac gctgtatttt
    11161 tttactaaat atcattttat ttctttgtgg acttttttag atatcgctgc accaagtgta
    11221 ttattagcac aggcaatcgg tcgttgggga aatttcatga accacgaagc atatggacca
    11281 gacacgacgc gaagcttctt agaaagtctg catttaccgc aattcatcat tgataatatg
    11341 tatatcgaag ggatctatcg tcaaccaaca tttctttatg aatcactttg gagtttgatt
    11401 ggatttattt tgcttttgtt tttacgaaag aaaccacacc ttttgaaaaa aggcgaagtc
    11461 cttttatgtt atctaatgtg gtatagtttc gggcgtttct ttattgaagg attacgtaca
    11521 gacagtcttt actttttagg tgtgatccgt atttctcaat tattatctgc tgtgttattt
    11581 gtcggtacat tgattctatt tatttggcgc agaaaaaaag gaaatttacc ggattatgac
    11641 cgctcattag gaaaaaatca acaaattatc tgatttttag agcgttaaaa atgaaaaaaa
    11701 tttcaggagt ggtaaaatga attatagagt agcggtttta ggaccaggct cttggggtac
    11761 tgctttggca caagtcctcg ccgaaaacgg acatgacgtt cggatttggg gacatcgtgc
    11821 ttcacaagta gaagaaatca acacccaaca cacgaatcaa cgttatttac cagatcatta
    11881 tctacccgat tccatccaag cattcaccaa tatggaagat gcagtaaaag acgcagatgc
    11941 ggtgctattc gtcattccaa cgaaagcaat acgttctgtt gcaaaagaac tagtgcctaa
    12001 attgaataca aagccagtga tcatccatgc aagtaaagga ttagaacagg gaacacataa
    12061 aaggatctct gacatcttgt tagaggaaat cccagcagaa aaaagaaaag ctgttgttgt
    12121 tctttctgga ccaagccgtg cagaagaagt agcaaaacat gacatcacta cgatcacagc
    12181 tgcaagtaca gatgcaaaag cagcaaaata tgtacaagag ctgttcatga ataattattt
    12241 tcggatctat accaatcctg atgtgatcgg tgtagagatg ggggcagctt tgaaaaatat
    12301 tattgctatt ggtgctggcg ctattcacgg cttagggttt ggcgataatg caaaggcagc
    12361 aatcatgacg cgcggattag ctgagatcag ccgtttaggt gtagcaatgg gtgccaaccc
    12421 gctgaccttt atcggattaa gcggaatagg cgatctagtc gttacttgca caagtgttca
    12481 ttctaggaac tggcgcgcag gcaacttgtt aggacaaggg cagcccctag aagaggtgct
    12541 agccaatatg ggaatggtcg ttgaaggtgt gaatactacc caagcagcga tggaactagc
    12601 ccaaatgatg gaaatcgaaa tgcccatcac tcaaacaatc tatgaagtgc tgtatgaaca
    12661 aaaagaaatc aaaacagccg caaaagaaat catgttacgt gatggaaaaa aagaaaatga
    12721 attctcatta acaatttttt gaaggagcga atgtatatga aagttaaaaa agcagttatt
    12781 ccagcagcag gattaggaac aagattttta ccagcaacaa aagctatggc aaaggaaatg
    12841 ttgccaattg ttgataaacc aacaatccag ttcatcgttg aagaagcgtt gaaatcaggt
    12901 atcgaagaca tcttgatcgt tacaggaaaa gctaaacgac caatcgaaga tcactttgat
    12961 tcaaatattg aattagaaac aaacctaagt gaaaaaggaa agaccgaatt attgaaatta
    13021 gtagaagaaa cgacagatgt caacttgcat ttcatccgtc aatctcatcc aaagggttta
    13081 ggacatgccg ttcttcaagc aagagccttt attggaaatg aaccatttgt cgttatgcta
    13141 ggcgatgata taatggaaga cgaagtacca ttgacacagc aattaatcga tgattacgaa
    13201 cgtactcatg cttctacgat cgccgttatg caagttcctc atgatgaaac ttcaaaatac
    13261 ggaattatca atccaggaga agtattggaa gatgggttat acaatgtcaa aaactttgtt
    13321 gaaaaaccag atccaagcaa agcaccaagt gatttagcta ttattggacg ttacttattg
    13381 actccagaaa tttttgatgt attgtccgag caacaaccag gagccggaaa tgaaatccaa
    13441 ctaactgacg caatcgatac attgaataaa acacaacgcg tatttgcccg taaattcaca
    13501 ggtaaacgtt acgatgtcgg tgacaaattt ggttttatga aaacaagtat tgaatatggt
    13561 ctaacacatc cagaagtagg tgcaccttta cgtgagtaca tcctgtcttt aggtaaaaca
    13621 ttagaagcag aaggaagcaa gatcgatcat aatccaaaag gaataacaga aatcaaagaa
    13681 gaaaagaaaa aataaccctt ttttctaacg agcttcagct gctaattgaa taaaaagtag
    13741 ttgaagctcg tttttatttg tttaaaaaac acgaaaatgc tatccttata gaaaagagag
    13801 acaagaaggg agtttgatca tgtcaggagg agaaattgca ggattgatcg cagcaatcgc
    13861 ctttgcgata cttgtcgttt ttatcgttcg tgtgttgttg caagtttcta aaactttaga
    13921 aaaagtagat cagactgtag cagaagcaaa tacgacgatc gaaatcgtca caaaagacgt
    13981 agatcttttg tctagacaag tagaaggctt actggtcaaa agtaatgaac tgctcttgga
    14041 tatcaatgga aaagtcgcaa cgatcgatcc attgttcaca gcagttgctg atttaagtga
    14101 aagtgtatca gaacttaatc attctagtaa aaacatcgcg acgaaagtcg gcggaatcgg
    14161 taaaacgaca gctaaagcga cggttgccgg aaaagtcggt gggacagcga tgaaattttt
    14221 taaaaacaaa aaacatactg aaacgacagg aggaaaataa aaatggctaa aaaaggtgga
    14281 tttttattag gtgcagtagt cggaggcaca gccgcagcaa tcgccgcgtt attgttagca
    14341 ccaaaatctg gtaaagaact tcgggatgaa ttagctgccc aagcggatga tttgatggat
    14401 atggcttcag attacacaga tatggcgaaa gaaaaatcga ctgagttaac aggtattgca
    14461 aaagaaaaag cgcaagatct ttctcaacaa gcaggtgatc tagcaggtag tgtcaaacaa
    14521 aaagtaagtg aatcagcaga tgaagcagga agtgaatctg acgatatctt gttaaatctt
    14581 aaagaacaat cagctgactt atctaatcgt ttcaaaaaaa cggcagaaga tatgaaaaaa
    14641 cctgcagatg aattaggaag tattgctaaa gatacttcag aagatatcat gattgatgta
    14701 aaagacgctg ctggtaaagc aggagatact gtcaatgaag gaatgtcaga agcaaaacca
    14761 gttgcagatg actcaaaaga aatgatgaaa atggcaaaag aagatgcgaa agaagctaaa
    14821 aaagaaataa aagatacaat gacagattca tctacaaatc actaaaatca caaatttata
    14881 gaataaaaag gtgggagcgg actagctctc acctttttta ctcttatttt tctaaaacaa
    14941 gtaattcttt tggtgtagtc gtgaatggca cacagccatc gcttgtcaca tatacacagt
    15001 cttcgatacg tacccctact ttttcaggaa tatagattcc tggttcaaga gagaagcaca
    15061 tcccttcttc gattactaag tcatttcctt ctacaaggga agggaattcg tggacggttg
    15121 ttccgatccc gtgacctaaa cggtgattga aatattcgcc gtagccatag ctgttgatca
    15181 cgtttcgtgc gatttgatca agttcacctg ctgtcacacc tggacggacg gcttcttgtg
    15241 cggcaagctg tgcttcaaga gtgatgttgt agattttttc ttggaagtcg gttggttttt
    15301 gataagcaat tgtacgagta gcatcactgc aatagccatt ccaaacaacg ccaagatcga
    15361 ataacacaag ttcattcggt gaaatcttcg tatttcctgg tgtaccatga gggcttgcag
    15421 catttgctcc tgcaaggact aatgtgtcga aagacatttg tgaaacacct tgttttttca
    15481 attggtattc aatttctgca atgatcgctt gttcagtcac gccttctttc actgctttaa
    15541 agccaatctc aaatgcaaca tcggcccatt taccagcttc gatcagtttg tctttttcct
    15601 ctagagtttt gatcagttgt agacgttgga tgatggaagt tagatcagtg taagaatccg
    15661 ttcccgtcaa gatatcagag atagcatcga agcgctcgac agtcaaggcg ttcttttcaa
    15721 tccctacatt gccaggctga ccatagcgag cacgaagttc ttttccgatg atctcccaag
    15781 ggttttcgct gtctaaatag ccacggacat catacgccca actactgttt tgagcatctt
    15841 caacttccaa agcaggagtg aaaaggaaag gatcttgttc aagagggata aaaagagcta
    15901 gtacacgctc atgaggatca ctttcgtatc cggaaaagta agcgatgtgg ccagggtcgc
    15961 tgatataagc caattcgact tggtgttgtg ccatccattg acgtagttca ttgattttta
    16021 cgttattcat ttggcaacct actttctaaa ttaattttgt attcaataat tgtaccactg
    16081 atttattaaa agaccaacgt tctaaaaact gaaaaaagta aaaattttca tgaaaacaga
    16141 gat
//
LOCUS       DAXWOO010000052        16117 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.052, whole genome shotgun sequence.
ACCESSION   DAXWOO010000052 DAXWOO010000000
VERSION     DAXWOO010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16117
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.052"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(115..744)
                     /gene="pyrE"
                     /locus_tag="ACGE70_001324"
     CDS             complement(115..744)
                     /gene="pyrE"
                     /locus_tag="ACGE70_001324"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFF8692971.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(751..1467)
                     /gene="pyrF"
                     /locus_tag="ACGE70_001325"
     CDS             complement(751..1467)
                     /gene="pyrF"
                     /locus_tag="ACGE70_001325"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFF8692972.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(1464..2396)
                     /locus_tag="ACGE70_001326"
     CDS             complement(1464..2396)
                     /locus_tag="ACGE70_001326"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFF8692973.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(2393..3169)
                     /locus_tag="ACGE70_001327"
     CDS             complement(2393..3169)
                     /locus_tag="ACGE70_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFF8692974.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(3223..6408)
                     /gene="carB"
                     /locus_tag="ACGE70_001328"
     CDS             complement(3223..6408)
                     /gene="carB"
                     /locus_tag="ACGE70_001328"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFF8692975.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(6413..7489)
                     /locus_tag="ACGE70_001329"
     CDS             complement(6413..7489)
                     /locus_tag="ACGE70_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFF8692976.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(7494..8774)
                     /locus_tag="ACGE70_001330"
     CDS             complement(7494..8774)
                     /locus_tag="ACGE70_001330"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFF8692977.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(8793..9719)
                     /locus_tag="ACGE70_001331"
     CDS             complement(8793..9719)
                     /locus_tag="ACGE70_001331"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFF8692978.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(9732..11015)
                     /locus_tag="ACGE70_001332"
     CDS             complement(9732..11015)
                     /locus_tag="ACGE70_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFF8692979.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(11029..11568)
                     /gene="pyrR"
                     /locus_tag="ACGE70_001333"
     CDS             complement(11029..11568)
                     /gene="pyrR"
                     /locus_tag="ACGE70_001333"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFF8692980.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(11803..12714)
                     /locus_tag="ACGE70_001334"
     CDS             complement(11803..12714)
                     /locus_tag="ACGE70_001334"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFF8692981.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(12711..13175)
                     /gene="lspA"
                     /locus_tag="ACGE70_001335"
     CDS             complement(12711..13175)
                     /gene="lspA"
                     /locus_tag="ACGE70_001335"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFF8692982.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(13180..13878)
                     /locus_tag="ACGE70_001336"
     CDS             complement(13180..13878)
                     /locus_tag="ACGE70_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFF8692983.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(13865..14251)
                     /locus_tag="ACGE70_001337"
     CDS             complement(13865..14251)
                     /locus_tag="ACGE70_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692984.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(14398..16068)
                     /locus_tag="ACGE70_001338"
     CDS             complement(14398..16068)
                     /locus_tag="ACGE70_001338"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFF8692985.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
BASE COUNT         4329 a         3719 c         2878 g         5191 t
ORIGIN      
        1 ttcttttcat acctgatgaa aagatgcttc tcgcaatcag tttttggatt tttgctattc
       61 caattgatga ttctggagac acaaatcgga tttctctatc ttcatcaagc gcgcttaaat
      121 ctgccaattt tcaggatctt ttttccattc ttttagcaga gttaattctt gctcatcgat
      181 ataacgatct tctaaagctg cttcaatcaa tacagaataa ttggtcaacg tcataagagg
      241 gatttcggct ttttcgaaat ttgcttttcc ttttggcaac tcatacgtaa agatagccgc
      301 aacaccaagg atatccgcac cttctctttt tgctgcttca gctgcttcca agacacttcc
      361 gcctgtagaa atcaaatcct cgatcacaac catcttctga ccttcaaata tacgtccttc
      421 gatttggtta cctttcccat gatctttcgc cttacttcga atatagacca ttggcaaatc
      481 cagtatttcc gctacccatg cggcatgagg gattcctgct gttgcagttc cagcaatcac
      541 ttgtacttca gggtaagctt ctttgatttt gcttgctaat ccttttgcga tctcttttcg
      601 gacttttgga tagctcatcg tgattcggtt atcacagtaa attgggcttt tgattccact
      661 tgcccatgta aaaggattac ttggatttaa aaatactgct tcaatttcta ataagtcttt
      721 tgcgatactt tgttcaacat tcattttagg ttagctccat tctgtttgaa tcgtttgata
      781 tgctttataa ggattagttg actgagtgat tggtcgacct ataacgataa aagttgagcc
      841 gatctttttt gcctgagaag gtgtgactac tcttgtctga tctcctgcga ctgttccttc
      901 tggtcttata cccggagtta agcaaacaaa ttcgtctcct gccatttctt tgatcgcttc
      961 tacttcccaa gcagaagaaa ccacaccatc taatcctgct tttttggcac agctagcata
     1021 atggatcaca cttttttcta aagaaacttc gattagttga tcggcatgca tttcttcttc
     1081 acttgtagaa gtaagctgag tgatagctag aagtagcgga cgttttccac cttctggagt
     1141 cccctcttct aatcctcgca ttgccgcctc catcatacga ataccgccag cggcatgaac
     1201 acaggtgaga tcaacaccaa gctttgccaa tccccgcatt gctttttcga ccgtattcgg
     1261 aatatcgtga agtttcagat ctaaaaatac attatgacca gcctctttta gataacgtac
     1321 gatttcaggt ccttcttggt agaacaattc cattccgatt ttcacaaaca atttttcttc
     1381 tttcgggaaa aatcgaagaa agtcttctac ctcttgccta gttgaaaaat ctaatgcaat
     1441 aattggtcgt tggtctagtt gattcatcgt tcacgttcct ctctcacttc tttgattaat
     1501 ctttccagcg attcgatgcc tagttcgctc attcgcacag ggagctcttt aattagttta
     1561 ggacagatat acggatcggt gaaattcgcg gtccctatag ctacagcact tgctccagcc
     1621 ataaacatct caagaacatc atccactgtc tgcacgccgc ccatcccaat gattggaagg
     1681 ctggatatac ttgctacttg gtgaatcaaa cgaatagcga caggcttgat tgctggtccg
     1741 gagagtccac ctgtttgatt ggctaaaatc ggttttcttg tcttaaggtc aatccgcata
     1801 ccaagaagag tattgatcat cgtaaaccca tctgcccctc cagcttcgat tgcctgagcg
     1861 attggaacga tgtctgttac atttggggac aatttgacat agacaggaac agaagaaact
     1921 ttctttactg cttgtgttaa ttgaaaagca acatcagaat ctgttccaaa agcaattccg
     1981 ccatgtttga catttgggca agaaatgttc agctcgattg ctttcacatt tggtgcttcg
     2041 ccaattttgc tacaaacttc tacatagtct tcttcacaag caccagcaac atttgcgatg
     2101 atgggtaact cattgtactc ctctaattct ggaagaagtt tatgcataac aacgtctaga
     2161 ccaggatttt gcaaaccaat tgcattcaac atgccagatg gtgtttccgc cactcttggc
     2221 gtgggattgc caaagcgggc atttggagtc gttgccttga ccatgatcga tccaagctga
     2281 ttcaaatcat agtattttcc gtattctttg ccaaaaccga aacagccgct tgccggcatg
     2341 ataggatttt ttaattctag gccaggaagt ttgaccgcta aaggattctc attcatataa
     2401 ttacctctcc tattttaaaa atcggtcctt cgtcacaaac tttcacactt tttgttcctg
     2461 tttcatcttc cggcgtatga caaacgcaag catagcaagc ccctatccca catgccatgc
     2521 gggattctaa tgataactgg acattttgat gcgaagaaaa cagctgttcc actgtcttca
     2581 ataacccgtt tgaaccacat gcaaaaacag ctgtaggttc cgtcttcgtt cctaacaata
     2641 aattaccgac atttccgtgc ataccgtatg ttccatcgtc agtagcgatt cttgtttctc
     2701 ctagacgatt aaattctttt tcataataaa cgacttcatg tgtggcaaaa cctaaaaaat
     2761 gcgttggctt aattcctttt tcgactaact gttttgataa ctcatataga ggagggacac
     2821 cgattcctcc accgattata aatacttcgt ctcctgtatg aagattggac aaatcaaaac
     2881 cattgcctaa aggacccatg acatctaaat ggtctccggc agaaagagta gagaagactc
     2941 tagtcccctc accttccacc cgataaatga tacggcaagt atttgtttcg tggtcgatat
     3001 gattcaagct aatcggtcgg cgtaatagca agtctgcacg cggcacgaga atatgcaaga
     3061 actgtcctgg ttgctgcatt tcttttacta gatcaccttt caatgtcatt tcaaaaatct
     3121 ttggtgccag ctgtttttgc tgaacgattt ccatcatttc ttgtttcatc tgtcccctcc
     3181 tgcaacggtt tttcgtcttt ttgtcttaac atgtgctcgc cgttaaatag ggttagttga
     3241 aaaagcacgg gattccatca ctcgaacgat cgcatctgct gtgtctaaag aagtaaacaa
     3301 tggaatacca tgttcaactg cttctcttcg gatgatgaaa ccgtctttag aagcatttcc
     3361 ccgatcttta tcaatcgtat tgacaaccac ttgtgtttta ccatctctta ttaagtcgac
     3421 tacatttttt tctgctgctt cctcagaaat tttcgctact gtttcaacga ccagaccatt
     3481 tttttcgaaa taagcagctg tatgtttcgt tcctatcaga ctatagccaa tttctgtaaa
     3541 acgtttagct aattctagtg cttcttcttt tgtttcgtcc gcaatcgtaa ataatacggc
     3601 tccatattca ggcaagtgta atccagaagc ttcgaatgcc ttgtacaaag ctttgtctaa
     3661 ttgtctgtca gatcccatta cttctccagt cgatttcatt tcaggcccta agtaagtatc
     3721 aactttctgc aatttcgtaa aagaaaagac aggtgcttta acatgaactt cttttgtttc
     3781 tggataaagt ccgttttcat aaccaagtgt ctttagtttc tctcccaaaa tagctttcgt
     3841 cgcaatttgt gccattggaa ttcctgtcac cttacttaag aatggaacag tccgacttgc
     3901 tcgaggattg acttcgatca cataaacctc attttcatgg atgacaaact ggatattcat
     3961 catcccgaca caattcaaac ctaatgctaa gcggatcgtg taatcttcga tcgttttctt
     4021 gatcgattca gataatgttt gaggtggata cacagccatt gaatcccctg aatggacacc
     4081 agcacgctca atatgctcca tgatccctgg gatgagtact gtttcaccgt cacaaatcgc
     4141 atccacttcg cattcaccac caagcaagta acggtccact agtactggat gttctggaga
     4201 tgctttcaca gcatgttcca tgtaatcttc taaatctcgc tggttttcaa cgatttccat
     4261 cgcacgtccg cctaatacat agcttggacg aacaagaact ggataaccaa ttctcgaagc
     4321 gataaccacg gcttcttctt tacttgtagc agtatcccct aaaggttgag gaatatccaa
     4381 ctctttcagc gcttgctcga ataaatcacg attttccgca cgatccaagt cttcgatcgt
     4441 tgtccctaag atttttacac cagcagcagt caaaggttcg gctaagttga ttgctgtctg
     4501 accaccaaat tgaacgatta ctcctatcgg ctgttctagc tcgatcacat tcatcacatc
     4561 ttcgaaggtc aatggctcaa aatataattt atccgaaata gaaaagtcag tcgaaactgt
     4621 ttcaggattg ctgttcatga tgattgcttc gtatcctgcc tgctggattg ctttgacaga
     4681 atgcacagtc gcatagtcaa attcaacccc ttgtccgata cggataggac cagatcccag
     4741 tactagaatt gagggctttt ctgatcgtga actttcattt tcatattcat aggaactata
     4801 aaaataaggt gtttttgatt cgaattccgc tgcacaagtg tcaaccatct tatatacagg
     4861 aatcaaatca ttttcttttc tcatctggcg aacggcttct tcgtttattt cccacagctc
     4921 cgccactttt ttgtctgtaa aaccgttttg tttcgctgtt ttcaatatct tgtcatccaa
     4981 cggctgctgt ttcaactgct gttcgatttc tacgatatgc aacagtttat ctaaaaagaa
     5041 taaatcgatt tttgtcaaat ccgctaattc ttctatggtg taccctcttc taatagcttc
     5101 agctacataa aacaggcgat catcttgtgc tcgaaccact ttttggatta ggtcatcgtc
     5161 cgtgatgtct gccaactcag gaagttcgat atggtgtgta ccgatttcta aagaacgcac
     5221 agctttcaat aaagactctt cgatattccg accgatagcc atcacttcac cagttgcttt
     5281 catctgcgtg cctaaaagcc gttctccgtt ttcaaactta tcaaacggcc agcgaggaat
     5341 tttagctaca acataatcta atgcaggttc aaattctgca aaagtcgttc ctgtaacagg
     5401 atttttcatt tcgtctaatg ttaatccaag cgctatttta gcagctagct tagcgatcgg
     5461 atagccagtc gccttacttg ctaacgcaga tgaacgagaa acacgcggat tgacttcaat
     5521 cacataataa ttaaaactat gtggatcaag agcgagttgg acattacagc ctccctcgat
     5581 cttaagtgct cgaataatcg aaagagaagc atcacgaagc atttgatatt catggtcaga
     5641 caatgtttga ctaggggcaa atacgataga atcacctgta tggataccca ccgggtcaaa
     5701 attttccata ttgcagacaa cgattgcatt atcagccgaa tctctcatca cttcatattc
     5761 aatttcttta aaacctgcaa tacttcgttc aatcaaacat tgggtagcgg gggaaagact
     5821 taatccattt tctgatacga tacgtaattc ttcttcattt tcacacatac ctccaccagt
     5881 tccgcctaaa gtaaaggctg gacgaacgat gacaggatag ccgattttat ttgcaaaatc
     5941 tacagcttct tctactgtat tgacgatttc agactcgggg attggctgat tcaattcttt
     6001 catcaactgt ttgaatagat cacgatcttc tgcttgatcg atcgcagaaa gctttgttcc
     6061 tagaagttct atgttcaatt cttctaaaat acctgatgct gccagctcca ttgccatatt
     6121 gaggcctgtc tgtccgccta aagttggaag gagagcatct ggacgctctt ttcgaagaat
     6181 ccttgataca aattcaagtg tgatcggttc gatatatact tgatctgcca cttctttatc
     6241 cgtcatgatc gtcgcaggat tagaatttac taaaaccact tcatatcctt cttcttttaa
     6301 ggcaaggcag gcttgtgttc ctgcgtagtc aaattctgcg gcttgaccaa tgatgatcgg
     6361 accagatcca atcaccataa ttttcttgat atctgtacgt ttcggcatct gtttactgct
     6421 cctttcctgc gtccatcatt tccataaatt catcaaataa atgtagtgca tcatgcggac
     6481 caggggcagc atctggatga aactgcacac tgaaagcggg ataatctctg tgtttgactc
     6541 cttctactgt tccgtcattt acttctacat gagttacgat gagtttttct ggatcaatcg
     6601 tggcttcatc gactgcatag ccatgatttt gagaagtaaa atcaattctt ccggtcgcta
     6661 cttctcgtac cgggtgattt aatcctcgat gtccgaattt cattttatag gtatctgcac
     6721 cgttggctaa agcaaataac tggtgaccga gacaaatacc aaaaatcggt acttttcctt
     6781 ggattttttt gatcatttcg attgcttctg gcacatcttt tggatcacca ggtccattcg
     6841 tcagcatgac tccgtctggt gatagcgcta aaatcgtctc ggcatctgtg ttatagggaa
     6901 gtactgtgag gttacagttt cttttactca attctcgtaa aatactatgt ttcaagccga
     6961 agtcaatgac aatgactttc cgacctgttc ctggacttgg ataaggtttg ttagttgaga
     7021 cttgctgcac ttgattggtt gctagaacgg ttgctttcag ctgatcaaat gcatgttcga
     7081 aggaatctcc agcatcaacg atactagctt tcatcgttcc gacttcacgg attttgcgag
     7141 taagggcgcg cgtatcgata cctgatattc caggaattcc tttttgtttc aaaaatgaat
     7201 ctagcgtcat ttggtttcgc cagtttgaag ctacacgtgc atgttctttt actactactc
     7261 ctttgcatgt tggagaaatc gattcataat catctctatt taccccataa ttacccacca
     7321 taggataggt aaatgtaatg atttgaccgt taaaactttg atcagtgata gtttcttgat
     7381 aaccagtcat acttgttgta aaaacgattt ctccgaagac actagcatct gcaccaaaag
     7441 cctttccttc gaaaactgtt ccatcttcta atataagcaa acgttccata cttttattct
     7501 cccttctgcc acacaagttt tccgttgaca aaagtcattt gtgtttcgcc ttttactttc
     7561 cagccgataa aaggtgtatt ctcccctttt gataaaaagt cttctttatc gatcgtgcaa
     7621 gtttgtgtaa tatcaaaaac tgctacatca gcgggggcac caaccgttag cgttcctgca
     7681 ttcaaaccaa aaatctcagc cggtttaact gccaaccaat tgatcacttg ttccaacgtg
     7741 aatcgaccag tttctacaaa atgagtatag atcagttgaa acgctgtttc gcttcctacg
     7801 ataccaaatg gtgatttcat gaaactttgg cttttttctt ctaatccatg gggggcatgg
     7861 tctgtagcga tgcaatcgat cgtaccgtca agcaagcctt cgatcaaagc ttctctgtcc
     7921 tcccgtcctc ttagcggcgg gttcattttc caaaagccga aatcttcggg aatatcttcg
     7981 tcgatcaaaa taagatgatg gggtgaaact tctgctgtta cgtggattcc tgcttttttt
     8041 gcgtctctga ttacacggac actttcttta gtagaaacat ggcaaacatg gtaatgaaca
     8101 ccagttgctt cagctagcaa cagatctcta gcaatttgag aagactcggt tacgcttaag
     8161 atcccgggaa gaccaagttc ttctgccttc ttgccagcgt gcataacgcc gccaaataat
     8221 aaagattcat cttctgtatg ggctactagg gctttattgt tttttgcagc ttctttcata
     8281 gccagataca tcgtcccagc tgtttggaca cctacaccgt catttgtgaa agcaaaagca
     8341 ccttcttcaa tcagtgcttc ttgatcaact aatttctcac ttcgcagatt ttctgtgatt
     8401 ggcgcatact gcaatacctt cacaactgca tcttttctaa tgatgtcata tacttgtctc
     8461 agcttttctg ccgtatcagg tacaggattt aaattcggca tcgcgcatac agtagtgaaa
     8521 ccgccacgag cagctgagcg agtcccagct tcgatcgtct ccttgtatgt aaatcctggc
     8581 tctctcaaat gaacatggac gtccactaaa ccaggtgtga tcaattgtcc ctttgcatca
     8641 aacacttcat caaattcggc ttctgagaat cctgtaccga ttgctttgat tcgcccatct
     8701 tctatccaaa tttctgctgg tgtcgtcata ttttttcgtg tattgatctg accgttttta
     8761 atgagtgttt tcattttttc atcctcctat aattacgctt ttccatttag cactgcttct
     8821 aagattgcca ttctgacgaa tactccattg ctcatttgct gaacgatccg cgactgtaat
     8881 ccttccacta atgaatcagc taattctaca tcacggttaa ctggtgctgg gtgcataatg
     8941 attgcatgtt tttgcaactt cttcgcacgc tcaactgtta acccatgttc ctgatggtag
     9001 ccttcttttg agaaactttc ttttccatca tggcgttcgt gttgcacccg aagcatcatc
     9061 atgacatcaa cttgatctag tagttcatcc aatggcatat aatgtccgta cacttcgaat
     9121 tcatcgtcgt accattctct aggacctgag aagaatactt gtgcgcctag acgttttaac
     9181 atctgcatat tggatttagc tacgcgagag tgagtgatat cacctacgat ggcaactttc
     9241 agaccttcaa aatgaccaaa ttcttcataa atggtcatca aatcaagcaa gcattgggtt
     9301 ggatgctgtc cgcttccgtc tcctccattg ataatcgaac actggattgt tttactttgt
     9361 atcagttcgt cataatagtt ctcatcacca tgacggataa ccgctacgtc tacgcctaat
     9421 gctgacatcg tcagtactgt atcgtatagt gtttcccctt tttggacaga gcttgtgctt
     9481 gcttcaaatt caatcacttc tagccctagt tttttctccg ctacatcgaa gcttttatgt
     9541 gtgcgagtac tattttcgaa aaagaggttt gtagcaaagt actggcgctt ttgcggagtc
     9601 caattagcac cttgcttgaa ttcctgccct ctgcggatca agcccataac ttcttgatca
     9661 gtaagtgcct caacggtcaa taaatgcttt aagctgatac gttctgattt gatgatcata
     9721 atgtttcctc cttagttttc ttcgcttcgt gaagtttctg gtaagataag atttaaaatg
     9781 atgccaagta cagttgctaa tgccattgcc gacaatgtaa atgtgcctac ttcaaagacg
     9841 agtccgccaa ttcctactac taagataact gaagcaatca acagattttt ctttttatca
     9901 aaatcgattt tgttctcgat taaaatcttc attccacttg cggcaattac tccaaataag
     9961 ataaaactga ttcctgagat gactggtcca gggatactta aaatcaaagc gcttaatttt
    10021 cctacgaatc ctaaaatgac tgcaaatact gcagctccac cgatcacaaa cacgctatgg
    10081 acacgtgtga tagccagaac accaatgttt tcaccgtaac ttgttacagg aggacctccg
    10141 acaaaggctg ccacgatttg tgctaatcca tcacctgtca acgtcttatg aagacccggt
    10201 tcttcaaaga aattacgttt agttagttta ttcaatacca taagatggcc gatatgctct
    10261 gtcatcgtca caaaagcgat gggtgccatt gttgtgattg ctccaagata tagctttggt
    10321 tcatattgta caaagggaat ctcaaagtta ggaacagcaa accaggcagc gtctacgatt
    10381 ggcttagtat ccacgattcc aaacagcaag gctacgatgt atccgctaat aatacctagt
    10441 aaaatcggaa tcaatccgag aaaacctctt agccacatgt tgaagaaaat tgttaatcct
    10501 aatgtgatca gcgctacagc aatatacttg aaatcatacg tgccctgatt atacatagca
    10561 tttgttgctg catttgccgc aagtcccaac ccgataacca tgatgactgg accgacgacg
    10621 attggaggca agattttatc cagccatgct gaaccgatct ttttgatgat cagcgcaaca
    10681 atcaaataga ctaatccagt tgtgactgct ccttgtgcaa tcgctggata accatctgtt
    10741 ttcatcaaaa gctgcatcgc tgcaatgaaa gcgaagctgc ttcctaagta cgcaggtatc
    10801 tttcctttag tagtaaataa gtacaccaat gtacctagtc ctgaactgac tagcgcaata
    10861 cctggatcga tacctactaa gatggggact aacacggttg ctccgaacat cgtgaacaag
    10921 tgttgtaaac ttaatcctac ccaatgtcct ggggttgggc gatcgtggat atctaacacc
    10981 acgtcatcat tatgaaatcc ttttttctcc gacatgtttc tctctcctct aatcttcctc
    11041 tttcaaaatc atgatgcgat cttgtccatc aagctcttgc atctctacta aaatctcttc
    11101 cgcccgagat gttggaatgt tcttcccaac ataatctgca cggattggca attctcgatg
    11161 cccacgatcg accaggactg ctaatgaaat ttttcttggt ctgccaaaat ccattactgc
    11221 gtccattgct gctcgaatcg ttcgtcctgt atacaaaaca tcatcaatca aaataacttc
    11281 ttttccttct aaggaaactg ggatgtcaga agaatgaagt tccggttctt ctggattttc
    11341 ttttttgtca tctcggtata aagtgatatc cagttctcct acaggaatat cgatatcttc
    11401 tagctgcttc aggcgttcgg caattcttga tgcaatgtag attcctcgtg ttttgatacc
    11461 taccaatacg atatcttgga tactatggtt tctctcgata atctcataag taattcgagt
    11521 caatgctcgc ttcatggtta cctgatctac tacttctttt gcttgcattt tcattccccc
    11581 tacttttttg cataaaaaaa ctcctgccct aagaggacag gaggaatgtt tagactgagc
    11641 gtatactgat acactgatag ctatactatg tcaatgagca atctacttat ggcgcaccat
    11701 acgtatcctt gctcccaccc ttcttagcct cacgggacta aacttaaagg aattctatta
    11761 agttttatgc tttcgttgtt aattactata ccgttatcta aattaaaatg caatgttttt
    11821 tcttaatttt tctaaagttt tttcaaaaac ttctggaaga ggtgcttcaa agatcatctt
    11881 ttcgtttgta gtcgggtgcg taaaacctaa catttgcgca tggagaaact gaccattgcc
    11941 tttcaaagtt ttcttcggtc cgtatactgg atctccagct aacggataac cgatatactt
    12001 catatgcaca cggatctggt gtgttcttcc ggtttctagt tttaattcaa ccagagtaaa
    12061 tccttcgaaa cgttccaata cagtaaaatg tgtgacagcc gacttcccat cttctcttat
    12121 cgcctgcatt ttccggtttt ccttcgaacg gccgattggc gcattgatgg ttcctttttc
    12181 gtgaggaatc tctccatgga ctaaagcaac atatttgcgc aaagaggtct tatcttttaa
    12241 ttgctgagct aatgattcgt gtgcctggtc atttttggca accattagca gtccggaagt
    12301 atctttgtcg attcggtgaa caatacctgg acgaataacg ccgttgatcg tagaaagatc
    12361 cttgatatgg tacatcagcg cattgaccat tgtgccatgc gtatgacctg ctgaaggatg
    12421 aacgaccatt ccttgaggtt tgtttatcac cgctacatct tcatcttcgt aaacgatcgt
    12481 caacgggata ttttctggga ccacatctaa tgtttctggt tcggggatca agacagtaat
    12541 ctcgtctcct tgttttactt tgtaatttgc tttgactggc tgtccattga cagaaaccgc
    12601 tccatttttc agccacagct gaatttgcga acgactgtaa tcggtgaata aattagtcaa
    12661 tactttatcg atcctaccag attcatggtg aatcgtcgtt ttaagttctg tcattttttt
    12721 ctttgatcct tttcatctaa aataatataa attaacatac aaatcactcc acatacaagt
    12781 gtcacatctg ctacgttaaa aatcggaaag ctcataaaat ctgtctggaa catatctact
    12841 acatagccaa gacgaatacg gtcgataaaa ttgccaatcg caccagctag aatcaaactc
    12901 aatccaatca tgaaccagcg attacctttt tcacgatttt taatcatcaa tgtgagaatc
    12961 acaacgactg caatgagtgt cactatagta aagaaccaca tcttcccttc tagcatactc
    13021 catgcggctc cagtgtttcg aatatgattc agtgacaaaa tacctggtat aagatttttc
    13081 gtctctccaa gcgctagatt atcagtgatc caccatttga ccaactgatc cactcctact
    13141 agcaaagcgc tgaatatcca ataaataatt aacaaaaaat caatcctttt ctatttgttt
    13201 ataaatatca cgaggtcgta cgatcccgag cagattacca tcgggattcc catctttagt
    13261 gataagtacg gcttccaaag tcccttgctc acgaaacatc gcttcaacct cgtaaatatg
    13321 tgttttagct ggaacaaaat gataatttgt gtactttccg ttttggatga tcaaatccaa
    13381 cgctcgttta tttgttaaat caaccggacc tttttggctt tcttttgcta gccaaaaccc
    13441 gatattacgt aaagtaatca atgctacaaa ttttccttta ttgtataacg gaaattgtga
    13501 ataccttttt tctgctacta tctttaacag ttcaagtaac ggaagatctt gttcaaaacc
    13561 tgttacacgt tttgcaaatc gaggcagcac ggtttcaggc tgaatcagtt cttgttcgat
    13621 cagctggatt cgcttgactg tccaatcatt tggctctgca atgacaaaat cgactgcgat
    13681 ccgatcatgg acgatcgcat tccttaactg agcaagctgt aaaagatcat cttcgtattt
    13741 tctgatcatt aattgttttc tttgagctag gcgtctgaca agttcggtaa atcccatatt
    13801 tcgtgcattt cccatctcgt ctctcatcca tttttcgatt cgattgaaac tgattaaaaa
    13861 aagatcagag tttgccataa ttttcctcct tgatcaaata tgcccggttt tttatataga
    13921 aaaagaacgc aatcaacaca ataactgcaa aaaaactgaa aaataaattt tgtaggaaaa
    13981 cggcccccaa cccagttgct agaaataaaa acggatagat ttgaagaatc aagtaattca
    14041 ttgtgatcac ataagcaggc tttgcttctt tcactgtata aacggggctt tttagccaat
    14101 aaaaggtgac ctgcatcgta aagggcactt ccagatcaag cgttatcttt tgttcatgac
    14161 tgagaacact gcgtttttca ttgttgattc ttagctgaat cgagctaccc ataccgtaaa
    14221 aaccggtttt tcttataaat gtcgttttca tcataccctc ctattatgct ttagtatagc
    14281 tcaaaataca ataaagaaaa agaatgatcc tagtgtaaac aaaattaatt ccctatcgtc
    14341 taaaaaaaga agctgtagtc aaaatacagc tccttcgatg gtagattata atcaagctta
    14401 aaacaatccc aacacttctc cgtcatttgt cacgtccata ttcaatgcag caggtttttt
    14461 aggcaaacca ggcatcgtca taacatctcc agtcaaggct actaaaaatc ctgcccctaa
    14521 ttttgggatg atttcgcgga tcgtgatggt gaaatcttcc ggtgcaccta gcagtgaagg
    14581 atcatcagaa aatgaatact gtgtttttgc catacaaatc ggcagtttgt cccatccatt
    14641 cttcgtaaac tcttctattt gtttttgcgc ttttttgctg aaaacaacac catttcctcc
    14701 gtaaattttc ttgacgattg tttctgtctt ttcttgtatc gtttcttcta ggcggtataa
    14761 tggcttgtaa ttagcttctt ttctatcaat caatcgtact acagcttttg ctaaatcaac
    14821 gccgccttct gctccatctg cccatacact cgctctcttg gcaagaatcc cctgattttc
    14881 acacaagtgc tccaacagtg tgagttcatt gtctgtatct gttacgaatt cattgattgc
    14941 gacgatcaca ggtagctgat attgttccat attacgtata tggcgcttta aatttgcaaa
    15001 acctactttc aaagcatcaa gattctcatt tttaagttca tcttttttca ttccgccatg
    15061 cattttcaat gcacggacag tggcgacaat caccacagca tccggcgctt ttttcaagtt
    15121 aggtactttg atatctaaaa atttctctcc gccaagatct gcaccaaatc ctgcctctgt
    15181 gacaacgtaa tctgctaatc tcaatgctgt cttggtagct aaaatactat tgcagccatg
    15241 agcgatattg gcaaacggtc ccccgtggac aaaagctggt gttccgtaaa ttgtttgtac
    15301 aagattcggc ttgattgcat ctttcagcag taatgccaga gcgccttctg ctttcaagtc
    15361 tccagcagtc acaggctgac ggtcaaatgt atagccgaca acgatccttg caagacgagc
    15421 ctttaaatct tcaaggtctg ccgcaagaca caagatagcc atgatttcgc tggcaactgt
    15481 gatatcaaac ccatcttctc ttggaacacc ttggattggc ccacccaagc caacaattac
    15541 tttacgcagt tcacgatcat tgagatcgac tacccgtttc caaatcactc ggcgggcatc
    15601 gatgttcaat tcatttcctt gatgaatatg attatctaaa agagcagata atgcattatt
    15661 agctgttgtg atagcatgca tgtcaccagt aaaatgcaag ttgatatctt ccataggcaa
    15721 tacctgtgca tatcctccgc ctgcagcacc acctttgatt cccatcacag gaccaagtga
    15781 gggttctcgc aatgcaataa cagatttttt attgatttta ttgagcgcgt ctcctaaccc
    15841 tacagtgatc gttgactttc cttcgccagc tggtgttgga ttgatggaag taactaagat
    15901 caaatgcccc tcagcgttat cctttgatcg attgattcca gaaaaattga ttttcgcctt
    15961 atatttccca tacagttcca aatcgtcttc cttaagatct aatttactag ccacttcaac
    16021 aatcggttct agctctgttt cttgtgaaat ttgcagatca ttcttcattt gattacccct
    16081 tttcacgaac tttattcaac aaaatatctt ctttgtt
//
LOCUS       DAXWOO010000053        15908 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.053, whole genome shotgun sequence.
ACCESSION   DAXWOO010000053 DAXWOO010000000
VERSION     DAXWOO010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.053"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(3..1304)
                     /locus_tag="ACGE70_001339"
     CDS             complement(3..1304)
                     /locus_tag="ACGE70_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFF8692986.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(1534..3525)
                     /locus_tag="ACGE70_001340"
     CDS             complement(1534..3525)
                     /locus_tag="ACGE70_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFF8692987.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(3669..3935)
                     /locus_tag="ACGE70_001341"
     CDS             complement(3669..3935)
                     /locus_tag="ACGE70_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFF8692988.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(4445..6571)
                     /gene="pnp"
                     /locus_tag="ACGE70_001342"
     CDS             complement(4445..6571)
                     /gene="pnp"
                     /locus_tag="ACGE70_001342"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFF8692989.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(6759..7028)
                     /gene="rpsO"
                     /locus_tag="ACGE70_001343"
     CDS             complement(6759..7028)
                     /gene="rpsO"
                     /locus_tag="ACGE70_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFF8692990.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            7279..7842
                     /gene="def"
                     /locus_tag="ACGE70_001344"
     CDS             7279..7842
                     /gene="def"
                     /locus_tag="ACGE70_001344"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFF8692991.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(8095..8616)
                     /locus_tag="ACGE70_001345"
     CDS             complement(8095..8616)
                     /locus_tag="ACGE70_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFF8692992.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(8619..9461)
                     /locus_tag="ACGE70_001346"
     CDS             complement(8619..9461)
                     /locus_tag="ACGE70_001346"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFF8692993.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKAVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(9454..10179)
                     /locus_tag="ACGE70_001347"
     CDS             complement(9454..10179)
                     /locus_tag="ACGE70_001347"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFF8692994.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            10355..10651
                     /locus_tag="ACGE70_001348"
     CDS             10355..10651
                     /locus_tag="ACGE70_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8692995.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      10907..11081
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            11219..12811
                     /locus_tag="ACGE70_001349"
     CDS             11219..12811
                     /locus_tag="ACGE70_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFF8692996.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            12824..13564
                     /locus_tag="ACGE70_001350"
     CDS             12824..13564
                     /locus_tag="ACGE70_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFF8692997.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            13732..15081
                     /locus_tag="ACGE70_001351"
     CDS             13732..15081
                     /locus_tag="ACGE70_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFF8692998.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(15209..15799)
                     /locus_tag="ACGE70_001352"
     CDS             complement(15209..15799)
                     /locus_tag="ACGE70_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFF8692999.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
BASE COUNT         4584 a         3278 c         3038 g         5008 t
ORIGIN      
        1 ttttattcta tttttctttc cgtggactct actttgaaat aatcgatatg tgcatgctgg
       61 tattctaata ccgtgaaacg gaatgatgca atctcaatga ttgttcctgc ttttatttct
      121 ttatatcggc tagaagcaaa gcccgcaagt gtagtaaaat tcgtttcttc aaactctgga
      181 atctctacat gaaaataacg ctggaaatca tctaatgtta gtttgccgga gatgatataa
      241 gagccatctg actgttccgc tatcaatgct tcgtccaaat ctgtctcatc tcggatttcc
      301 ccgacgattt cttctaaaat atcttcaatc gtcactaagc cgcttgtccc tccgtattca
      361 tcttttaaga tagcaatatg ctttcccttt tttcgcatga tcgtcaataa tttttttata
      421 gggatcgttt cagttgttac gatcggctct tcaatcaact gttctactgt cagctggtca
      481 tctttataag attgcttgat caaatctggc aaggtgacat aacctaaaac attgtctttt
      541 gattctaaga taaccggata gcgagtatgt ccttgttcaa tcgaagcttt tatcgcttct
      601 ttgacggttt ctgaagcttc gaatacttgc atggacgtac ggtctacttg tatatctttt
      661 gccaaggtat catcaaattc aaacacgttt tccaaataat gatattcttc tttggtcaac
      721 tctccttttt cataagaatt caatgcgact aatgtcagct cttcttgaga caatgtctct
      781 tctccttcag atacgagacg cacacctaat agcttcccta gccctgcagc tgaatgattc
      841 aatacccaaa taaaaggaaa cattacttta taaaaataat gaagtggttt aactacgaac
      901 aagacgacct tctccgtatt gacgatacta tagctcttag gaagcagttc gccaataacc
      961 acatcgatat acgtaactaa aataaatccc agcgccacag agattgtact gctaatagcc
     1021 gaactaaaag gtagtttacc gatcactggt tctagtaaag ccgcgacaga tccttcaccg
     1081 acccacccga tgatcaaacc ggttagcgta attcctaatt gactagctga taaatagtta
     1141 tctaaatggt gtgtaacatg aagagctagc ttggcgttct ttacattttc cttttctagt
     1201 tgttccaatc ttgatcgtct gattttcact aaagcaaatt cacttgctac aaaaaatgaa
     1261 gtaattgcaa tcatcaaaaa taaaataatg aaattgataa ccatattctc ccgcctttct
     1321 ctttttctat tgtacccttt ttctacaaaa agattgaaaa taatgcgtat tcatatttag
     1381 gaataaaaaa ggagatgcgg catttttcag ccacatctcc ttatcacttc aaaatcattg
     1441 cgttacttgt tgctaaacag ctttttcatg acctctgata aacggtgttc aagtagctga
     1501 cattcgacat tagttcaggt caaagcgcta ctttcacaac cgctctttca accatgtttt
     1561 ccgagtattt ccaatgataa gcggaacagt ttcatgtttt acttcgctgt actctagccc
     1621 aaaccatgtt tctggcgagg tagcgatttt tttgattgcc agttttgcct gcatgatttg
     1681 acgttgccat tttcctagag ttgtcacatt ggaaactact tcatcaatca ggacaaattg
     1741 gaaatcgcca acttctcgcc ctggtgtcag agagtatctt tgtggctgtt ttggcaaacg
     1801 gccttgtttc atcaaatcat ggatgacttg gcgaacatat acgttgattt cctgttggac
     1861 cctgaaacct aagaacagat tcacttggac aataaaatca gtgtccatca tatctacttg
     1921 atattctttc gtatatggct catccgtcac ttccacatga acaaaccaat agactcgtgc
     1981 tcttttaggc cgtttatcta aaatcgaata gatgaactgt cggccaactt tatcttcagc
     2041 catatcagga accaagaaga ccacatttgt ctgatagatt ggtaaagaag tatcatcacg
     2101 taattctcta agctgcggga tataatctct caaagacaca tcctcggttg acgattcgcg
     2161 aataatgttc ccttgttccc aaataaacat cacgactaag atgatcaaac caatcagaac
     2221 ggctacaaaa ccgcctcgga agaatttgac aacacttgag ataaagaaaa cgacttctat
     2281 cgcaccgaag aaaaaggtaa ctaaatatgc aagccaacga gaatgcccat tttggataag
     2341 ataaaagtaa agcagtgctg ttgtcatcaa catcgtcaca gtgatcgata gaccgtaagc
     2401 agcttccata tgatgagacg ttcggaatgt gacaaccact agagaacagg cgatccataa
     2461 aatcagattg actgctggga tatacatttg cccgatactg ttcccaggat acatgatctt
     2521 taaccgaggc agcagtttca acttgattgc ttctgaaact agcgtaaacg aacctgtaat
     2581 caatgcttgc gaagcaatga ctgcagcgac tgttgcaaaa gcaacgccga tcacgatcca
     2641 actttgaggc atcatttgga agaatggatt caggttcttg atttgctgtg cttcagggtt
     2701 ttgataaaca ttcaacaacc acgcagcctg cccaaaataa ttgataacca aacaaatttt
     2761 gacgtaaggc cagctcagac gaatatttcg tttgccgaca tgtccaagat cggaataaag
     2821 cgcctctgca cctgtcgtag ccaggaaaat atttccaaga atgaacaagc ctaatttgtt
     2881 atctgcacta aaaagcagct ggatagcata ataaggattc agtgcacgga taactgtcca
     2941 gtcttgactg aagttcatta atcccattac gcctaaaaaa gtaaaccaag cgaacatgat
     3001 tggtccgaat gcttttccga caatatctgt accaaagcgt tggaccatga ataaaacaaa
     3061 aataataact aaagtgataa tgacgatcac gttctggtta ttaccgaacc ggtcaaaaaa
     3121 ctgcggaatt cctcgcaagc cttcaatcgc agtcgttacc gtcacagcag gggtcaatac
     3181 cccatctgct agtaaggcag ctcctccgat cattgcagga atgatcaaat atttcccacc
     3241 ttttcggaca agtgtgtaga gtgagaaaat tccaccttca ccatggttat ctgcattcaa
     3301 tgcaatcacg acatatttga ttgttgtcag tagagtcaat gtccaaaaaa tcaatgagac
     3361 tgatccgata acaaaatcag gattgattcc tgacaatccg ccattatctt caacaatcga
     3421 tttcataacg taaagcggac ttgtcccgat gtctccgtag acgactccca tcgctacaag
     3481 caggccagcc attgatatct ttttggtatt gtgcgttgtt gccacgtttt tccctcattt
     3541 cttgttatat ggatatcctt taggtaagct tgaatgatag atgatattct tatccatgac
     3601 acccgctttc ctaaattgct tctaattatc caagtaacac actgaagtgt caacttattt
     3661 gaataaaatt attctttctt tggacgttct ctgatacgtc ttgccacaaa tgaataaaga
     3721 gcaaggcaaa aaattgcacc caataatgct ggtatcaaat aaaaaccgcc gagttccggt
     3781 cccagtggtc ctagtaaaaa ttcgccgagc caactaccta aaaaaccgat aatgatattt
     3841 ccagatacgc ctcccgggac ttcctttcct aaaattgcgc cagctatcaa acctaatata
     3901 ccgccgataa ataacgacca taaaaaattc agcatctcct ttccctcctt tatccatatt
     3961 tctcactttt attttacgct ttattcagaa ggactccaaa aatatgcgcc aagaaactcc
     4021 agaaaaaaag aagttgtaac aaaagtgttc agcttatttc cgtagaagct gcttctggaa
     4081 caccgtgaat cagaggttgt gaaatcagcg gtttgacctg agtattagag aaacaaacgg
     4141 aaaaatagtt cctcatattt tgccgttttt tgagctaata tcgagggaca gctgattgaa
     4201 catcgtttat tggagaggtt gtaaaaaagc tgttctgcat caagtaataa gaacagccaa
     4261 gtaaaaacag ctcctaatgt ttttaacttg gctggactta ttaccgcaga tgcaagcttt
     4321 tgaacaccgt ttattcgaaa aaaaaggact gtgacacggc ttttgtcaca gtcccctttt
     4381 ttcgaataat tattgtcccc ctgtcacctt cacgatcaca agtacattat tcctatgaat
     4441 taaattattt ttcttcattt ttcgttgaac catcttcgtt caacattgct tttcttgaca
     4501 gatttacgcg tccttgttta tcgatttcag ttactttcac aaggatctca tcacctagtt
     4561 taacaacatc ttcgacttta ttgacacgat cgtttgctaa ttgggaaata tggacaagtc
     4621 catcttttcc tttgatcaag ttgacgaatg caccgaattt ttcgatacgg acaactttcc
     4681 ctagataaac ttcaccaact ttgacttctt tggtcaaatc ttcgatgatc ttgatagctt
     4741 ttttgatcat ttctgcatca gctgaagcaa tacttacttt tccatcttga tcgatatcga
     4801 ttttcacacc agtttcatcg atgattccgt tgatcgtatc gccgccttta ccgataacat
     4861 ctttgatttt agcaggttcg atctggatca tttcgatttt tggtgcatat tggcttaatt
     4921 cttcacgtgg agcagcaatc gttgaagtca attcttctaa gatttccata cgtgcttgtt
     4981 ttgcttgagt taatgcttca gttaggattt gttctgtgat tccttgaatc ttgatatcca
     5041 tctgtaaagc agtgatacca tcttttgtac cggcaacttt aaagtccata tcgccaagat
     5101 gatcttccag cccttggata tcagttagga tcgtatagtt ttcaccatca gaaacaagtc
     5161 ccatcgcaat accggcgact ggcgctttga ttggtacacc tgcatccatc aatgctaatg
     5221 tgccagcaca gatacttgct tgggaagaag aaccatttga ttccaatact tctgctacta
     5281 aacggatcgt ataaggaaat tcttcttctg aagggattac ttgtgctaac gcacgttcac
     5341 ctaatgcgcc atgtccgatt tcccgacgcc ctggagagcc tgcacgtcct gtagaaccaa
     5401 cagaaaattg agggaaattg tagtggtgga tgaatcgttt gctgacttcc acccctaaac
     5461 catcaatgat ttggtgctct cctagaggtg cgagtgtaca agctgataaa gcttgtgttt
     5521 gtcctcgtgt gaacaaacct gatccatgta cacgtggcaa taagctgacc tctgatgaca
     5581 atggacggat ttcatccaat ttacgcccgt caggacggat cttatcgatg gtgatcaatt
     5641 cacgtacgac atctttttca agatcttctg tgatttgttt aacttctttc aacaagtgat
     5701 cttcatcttc atgacctgca aatttctcag cataagcttc gcggatatcg atcttcacct
     5761 gctcgatatt atcttcgcga gcaagttttt cttcagtcat tactgctgct ttcattgtag
     5821 cgtaagaagc atcaaagatt tcttttttca atacttcatc aacttgcagt aaagtcactt
     5881 ccattttttc tttccctact gcttgaacga tttcttcttg gaaagcaact aactctttga
     5941 tagcgtcaaa accaaacagc aatgcaccaa gcatgtcttc ttctgaaact tctttcgcgc
     6001 cgctttcaac catattgatg gcttgtttcg ttccggcaac agtcaattcg atatctgttt
     6061 gttctgcttg ttctactgtt ggattcaaga cgtactcgcc atttacacga ccaacttcta
     6121 cgcctgcgat tgggccgtca aatgggatat ctgagattga tagtgctaaa gaagaaccta
     6181 gcattgctgc catagctggc gagcagtctg tttctacact catcacgatg ttcgttactt
     6241 ggacttcatt acggaagcct tctgcaaaca tcgggcgaat tggacggtcg atcaaacggg
     6301 cagtcaatgt tgcttccgta cttggacggc cttctcgttt gatgaaacct ccagggattt
     6361 tccctgctgc atacattttt tcttcataat tgatcgttaa tgggaaaaag tctgtatctt
     6421 ttgcttcttt agaggccaca gctgcactta agactactgt atctccataa cggactaaaa
     6481 cggctccgtt tgcttgtttt gctagctgac cgacttcgat ttgtaaggga cggccgcccc
     6541 atgtcgtttt gaatacttgt ttttctgaca taacttctcc ttttccttct cgtgaaagca
     6601 agacgctact aaacaaatga aaactgccga aagccgtctt ttgagcagct ttcatttggt
     6661 tagtaggagt ccatctagct tgatcacgga attttactta attgatatac gagtaaaaaa
     6721 agtgagattc atgtgaatcc cactcttttt gactaaaatt aacgacgcaa gcctaaacgt
     6781 ttgatcaatt cgcggtaacg ttgaacgtca gttttacgta agtaagctaa caagttacgg
     6841 cggtgaccaa cttttttcat taggccgcgg tatgaatgat ggtctttttt gtgcacgcgt
     6901 gcatgttcat tcaagtggtt gatttcttca gttaatacag cgatttgtac ttctggagaa
     6961 ccagtatccc cttcatggcg agcgtattcg ttgatgattt cattcttgcg ttcttttgaa
     7021 attgccattt ctttcacctc tttcgattat tcccctgcga ctgagtaaaa cgttggtgat
     7081 tcgcttaacc aagtggcagg tcatgtgcct agagcacaca gctatacttt acctgaaacc
     7141 gggttgaaaa tcaagtagtt aatcattaag aaaaggtagt tttcaaacaa tcttactact
     7201 acttttccat ttttgtggta aactataatg cgaaaataga cttgaaacac agtcttttaa
     7261 gaaaggggta catattccat gattacaatg gatgatatca ttcgtgaagg aaatccgacc
     7321 ttacgcgaag ttgcaaaaga agtttctctt ccattaagcg aagaagatat ctctttagga
     7381 aaagagatgc ttgagttctt aaaaaacagc caagatccaa tcaaagctga agaactacac
     7441 ttacgaggcg gtgttggctt agctgctcct caattagata tttctaaaag aatcattgct
     7501 gttcacgtac caagtcctga cccagaggca gatggacctt ctctaagtac tgttatgtat
     7561 aatcctaaaa ttttgagcca ttctgttcaa gatgcttgtt taggggaagg tgaaggctgt
     7621 ttatctgttg atcgagaagt accaggatat gttgttcgcc atgccaaaat cactgtgtct
     7681 tattatgata tgaatggtga aaaacacaag attcgtttga agaattatga atcgatcgtc
     7741 gttcaacatg aaatcgacca tatcaacggt gtgatgttct atgaccacat caacgatcaa
     7801 aatccatttg cactaaaaga aggcgtattg gtgatcgagt agagaattgg caggttcctt
     7861 cgatactatg ctacaagaaa gctttataaa aaaggggagt gctcaaaagc tgcacttccc
     7921 ttttttatgt ttctccacta atcgaaattt agatagacat ctagaaatcg caattccaaa
     7981 aaattctgtg tattggatac gcatcttttc ttattttttc ttatttcttc catatgggtg
     8041 tttatctcag agatgataga atagtttcta ggataaaaag tagcagctac gatgttatcc
     8101 ttcgtagagg attcctgctt cagctagttt ttgtttgatg tttttcagca tatgcttttc
     8161 tgggtagcgg atcgtatgca gatggatgcc gcctgttaat tcggataaca aagtagcatt
     8221 acttttttga taacgtctca taaaggaatc gatatcttgt ggcgttttga tgtgcaaagt
     8281 tcctgctaac tcaccgtaaa ggggatgttc cacaattaca tcaatgattt caccaccatt
     8341 ttcaacgatt agtctcagtt cttcttctgt ttgatctttt ccatgctgga cagcgatttt
     8401 actgatgtgc ccagtattgg tttcttctgt ttcgaggata taacctcttg ctgttgccac
     8461 gatactttta cctgcagctc gtagtaaagc aatatcgcct acaatgatct gacggctgac
     8521 tccaaaagtt ttcgcaaact tgctagcact cactggtttt tctgtatttg ccaaggtttg
     8581 catgatttgt tttcgccgtg tctctccttc gatcacactt attcctcctt cattttgttt
     8641 tggtattcgc gcgagtgttt catcaaatca acatacgtat ctgttattgt ttccttatat
     8701 cccttatttt tcagcagact aacaacttta tccaatactt gctgctctct ttttgcatca
     8761 taaactgctt ggttttcttc ttttttcact tgtcctattt tagttaccgt atccattctt
     8821 ttttctaata aagcaaccaa ctctcgatcg atcatatcga tcttatgacg taatgtttca
     8881 agagactctt ctgttagttc ttcctctact gccttgatga tacgagcagg atttcctgca
     8941 atcactacat tgtcgggaaa agatttggtc acaaccgaac ctgcgccgac tactacgttg
     9001 tcccctagag tgacacccgg cgtgacgata actcctccgc ctaaccaaac attattaccg
     9061 attttaatgg gttttgcgta ctccagaccg ctgttacgtt tcactggatg tagtggatgc
     9121 gtcgccgtgt aaagctggac attcggagcg aacatgcagt tatcaccgat ttcaatcgtg
     9181 ctgacatcta aaaatgtaca attgaaattt gcataaaaat tctccccaac aaatatatta
     9241 tatccgtaat caaaattgat aactggctcc atatagattt ttttccctgt acggccaaaa
     9301 gtctctttca acaattggga tcgcagttcc gctgattctg cttggttgat gatttggctt
     9361 tgacttcttg caaacatacg gtcggcaacc agctcaggat cattggcaaa ataaagctcg
     9421 cctgcaatca ttttttcttt ttcggttttg aattcagtca taatctgctc ctaattcttg
     9481 tatttttttg aaaacaaccg ctccgtcttc ttgaataaac gtataaggct ccgctaaata
     9541 aacaggcgtt tgattgatta ctaagatttt ggctgacggc tggcgaaact gtaccagatc
     9601 acaaaacgga tgaacttgaa aactcgttcc aacaatgacg atcagctctg cttggctgac
     9661 ggcataagcc gcctgctcca tgactccatc ttgaaaccct tcaccatata acacgatgtc
     9721 tgggcgtatt tgtcctccgc atgcttgatg tttgtcgctg tgaagatatt ctttccagtc
     9781 taccgtttgt tgacattttc gacaatgaca ttgatacaaa cttccatgaa aattgacgag
     9841 ttgcgggctt ttagcctttg cgtgcaaacc gtcgatgttt tgggaaacaa cccaaacatt
     9901 tttttctttc gctagatgtg ccatttccaa atgaatgata ttgggttttg cttcaggatg
     9961 ataaatcttt ttgacaaatt gatagaattt atccggttca ttcaccattg cttgatgact
    10021 cagcaagtat tccggctgct ccatcccgtg gtagattcct gacaaagatc gatagtctgg
    10081 cactccagaa ggtgtggata cccctgcacc ggtcaaaaat gtaatttgct gataagctaa
    10141 tatctctttt gctgcttcgt ctactgttaa ttgtttcatg ttttgtcacc tcactttcca
    10201 ttatagccca tactcgaaca aatttattgt tgaaaagtca gaaaaatggg aataaaaaag
    10261 tgttatgggc atttgaacaa tttatcacaa tgttcactga caaaagaatg aaaacgtgtt
    10321 acaatactgc tgtataaaag tgaggtgaat cgatatgtta ccattcttta tttattgggg
    10381 cgttattctg gcttgtatcg cctggttagc gttaagcgtt tatttctctg ttttttatct
    10441 tgtacgcaaa gaaaatggaa atctttgggc ttttgcttta ttcaacgtga ttgccgcaat
    10501 tgttttagcc attacattag ccgtatatcg aacatggggc tggggaatca cccaatactc
    10561 aagtttgatt tatctaatat tagccattta tggcgtcgtt gttcttttgc aagcgatcct
    10621 aggacgtgag ccaaagaaag cggcagctta aatgatcgag atggaactgg acacaggaaa
    10681 ttttctgttc tagttctttt tttattgtat tacatacatt tttgtgtata attatactat
    10741 cgtttgcaat gaatagatga ttcacatttt gattgttttt tcggtaaaag taaagatttc
    10801 tatatttatc agtatttttt caaaaaacag cagttataga ttgacaaagc ctctcgagta
    10861 tcgtaaactt tttttgtata aataactgaa tacgagatgc ctcaatcaat gaggtagagg
    10921 tcgcgagaat cattaaccct gtggagtgaa gcaaacatgg agaagcagga atagggagga
    10981 tcgccgaaat gtgcactttt ttgcttggag tgtatgttgg gacgcaagtt aagagcttgc
    11041 ggactgtctt agcagtgatg ctaagttgcg ctatgttttg tgagaggttt atcaccttat
    11101 ggaattttag cggccttttt atatctataa atcggtcgct ttttattttt tcagataaag
    11161 ctttaccgca tttcatctag accgctcgtg tatcatataa tcagaaaggg agaaaaaaat
    11221 gaaaaaaaat atggtttcag ttgccgtact atttgctttt gtcttctcac tattcagtgt
    11281 gggatctctg gcaaatgcag atgaaaaaac accgagcgat gagtttcgcg taggtatgga
    11341 ggctgggtat gcacccttca actggtctca acaaacagat gagaataatg ccttgccaat
    11401 tcaaggccaa aatagttacg ccggcggtta tgatgttcaa atcgctaaaa aagtagcaga
    11461 tggtttaggg aaaaaattag tcatcgtcca aacgaaatgg gatggtctgg ctcctgccct
    11521 tcaatcaggc aagatcgacg cgatcatcgc cggaatgagc cctactgctg aacgacgaaa
    11581 agaaattgat tttaccgatc cttattacga atcacaatta gtagtagtag ttcaaaaaga
    11641 tagcaaatat gcaaaagcaa aaagtttagc agatctttct ggagcaaaga ttactgctca
    11701 gttaaataca ttccactata ctgtgatcga tcagatacca gatgtgcaga aacaacaagc
    11761 aatggataat ttctcagcaa tgagaacagc tttagcttct ggaatgatcg atggttatgt
    11821 cagcgaacgt ccggaaggtg taacggcaac tagtgtgaac aaagacttgg aaatgcttga
    11881 attcagcggt gcagatggct tccagacaaa cccggaagat gttcagattt ctgttggtat
    11941 gcgtaaaggc gacccggata tccaaaaagt aaaccagatt ctttctggca tctctaacga
    12001 tgaacgaacc aaaattatgg accaagcagt caaagatcag cctgctgcaa ctgatagtga
    12061 agaaaaacct ggcatcattt ctgatttcac tacgatttgg aaacaatacg gcaatatgtt
    12121 cttacgaggt gctggactta ccttgttcat cgcattgatc gggacagttg tcggtactac
    12181 acttggatta ctgattggtg tcttccgttc ggtacctgaa tcaggaaatg cactgactcg
    12241 cttcttccaa aaaatcggaa atgcacttct ttctatctat gtcgaagtat tccgcggaac
    12301 accgatgatg gtacaagcaa tggtcatctt ctacggtctg gcactcgctt ttggcatttc
    12361 attgaaccga acgattgccg ctttgtttat tgtctctctc aatactggag cttacatgac
    12421 agagatcgtt cgtgggggga tttttgcagt agatgaaggg cagttcgaag ctgcgcaagc
    12481 cattggtatg acccatggcc aaacgatgag aaaagttgtt attccacaag ttttacggaa
    12541 tatcttgcca gcaacaggaa atgagtttgt tatcaatatc aaagatacag cagttttaag
    12601 tgtaatcggc gtagctgatt tgttcttcca aggaaactct gcttccggtg cgaatttcca
    12661 attcttccaa acatttacga tcgtcgggat catctactta gtcatgacct ttacgatcac
    12721 cagaatcctg cgggcggtag aaaagaaaat cgatggtcct tcagcttata ccaagcttga
    12781 agaaatcgac aattcaaatt tgcaggaata aggaggaaga atcatgaatg aaaaaattat
    12841 tgaaatcaaa catctaagaa aaagcttcgg tgaaaatgaa gtattgaaag atatcagcgt
    12901 aacagtcaat aaaggtgaag ttgtgacgat catcggttct tccggttctg gtaaatctac
    12961 tttcttgcgc tgtatcaatt tactggaaaa accaacaggc ggcgagatca tttataatgg
    13021 agaaaatgta ttagctccta aatataattt acctaaatat cgaacgaatc taggtatggt
    13081 tttccaatcc ttcaacctgt tcaacaatat gaatgtcttg gaaaactgta tgtctggtca
    13141 aacaactgtc ctgaaacgag ataaagaaac tgccaaaaaa gtagcaatcg aaaatctgga
    13201 aaaagttggt atggatcgtt atatcgaagc caaaccagca caattatctg gtggacagaa
    13261 acagcgtgtc gcaattgctc gggcattatc aatgaatcca gatgtattgc tattcgacga
    13321 acctacttcg gcgttagacc ctgaaatggt tggcgaagtg cttaatacga tcaaagactt
    13381 agcacatact ggcttgacaa tgcttatcgt gacgcatgag atggaatttg caaaagatgt
    13441 atctgaccgt gtcattttta tggataaagg tgtgattgca gaagaaggta caccagaaga
    13501 tatctttgtc catccgaaag aagaacgtac aaaagaattc ctttcacgta ttttaaatgc
    13561 ttaggatcaa tagctgattg ggacaaagag ctgcggtgtt taccgcagtt cttttttatg
    13621 aattactcat aaaaggtaaa tttctgaaaa ttttggaatc ttagttgtgt ttttcagaaa
    13681 actcttttaa tatggaagta attaaacttt tttagttagg atgatttaaa aatgaatgaa
    13741 gagcaagaac tgcagcgtgg actgaaaaat agacatgtcc aactgatttc tattggcgga
    13801 gcgattggaa cggggttgtt tttaggttcc ggaaagtcta tccagctagc tggcccttcg
    13861 atattgctcg cctatcttat tactggatgc atctgttttt tcattatgcg tgcgttaggc
    13921 gaacttctgt tatcaaacac caataaccat tcttttctgg attttgttgc agaatatctt
    13981 ggcaaaaaag cggctttcat cactggctgg acgtattggt tctgctgggt atcgattgca
    14041 atggccgatt taacagccat cggaatgtat gtacgctact gggcgcctgg tgttcctcag
    14101 tggcttcctg aactgattgc attaggtctt cttctttgct tgaatatggt ggctgtctca
    14161 ttgtttggtg agttagagtt ttggtttgca ttgatcaaag ttgtagcgat cattgccttt
    14221 atcctcgtag gagcttatat gatcctcacc cactatacga cagatattgg cacagccagt
    14281 atcacgaatc tatggtctca tggcggattc tttccaactg gtgcaaaagg atttatctta
    14341 gctttccaaa tggtaacctt tgcttttgct gggatagaat tagttggatt agtcgctgga
    14401 gaaacagaaa atcctgaaaa agtgttgccg gaagctatca ataatattcc tatcaggatc
    14461 atcttatttt acttaggatc tctttttgtc attatggcaa tctatccatg gaacagcttg
    14521 aatccagata acagtccatt tgtagaagta ttttcggaaa ttgggatcac gattgctgct
    14581 tccttgatca atttagtcgt attaagtgca gcagcttctg cctgcaacag tgcaatatat
    14641 agtacaggac gtatgcttcg ttctttagcg caagaaggca gtgcccctaa aaaattcaaa
    14701 aagttaacaa cacatcatgt gccagggaat gctttgacat tttctactat cgtcattttc
    14761 atttctgtta tcttgaatta cgtgatgcct agtgaagtat tcacactagt aagcagtatt
    14821 gccactactt gtttcttgtt tatctggagt atgcttgtct acacgcacat gaaatatcgt
    14881 aaaagtatcc tcggcaaaaa agctcattca tttaaaatgc cgttgtaccc atttagtaat
    14941 tatctggtat ttctttacat ggcctttgtg tgtgtcgtct tgttcctagg aaaagatacg
    15001 agaatcgctt tattactaac acctgtgtgg ttcttgcttc tattactaat ctatcatatg
    15061 aaatatgaac gtaagaagta agaaagtagt tgtgacagaa gtggtcagct tcgagaaata
    15121 agaaggaatt tacgaaaatt gcttcttaaa tttttgtgaa atttcggctt atttcttgga
    15181 gttaaaagct tctgtcccag cctcttttct atttccccgg aaacaaccgc tgataatagg
    15241 catctgcttt tttataggaa aaaatccaaa taccagatac aaaaaaacgt ccgaataacc
    15301 cgatcaacgg tacaaaaaag acaccggcaa tcaagcataa gttaatcatc atggagataa
    15361 gacttcgacg gtcctttgac aaaactctca cgacatggga atctggttta tttgctttca
    15421 tgatttgcct catcaagata ccaaaagaaa aactccacat cgtgtatact aagacataag
    15481 ccatctcagg ccaaaaagct tcagggttac gcccgaccca actagtaaca gaaggacata
    15541 aagtcagcca aaagagcaaa aagatattcg accagaccac tcggtagtta attcgttctg
    15601 ccatctgaaa tatacgatga tggttcaccc agatcaccgc aacaaatata aagctgatgg
    15661 tataagctaa taatgtattt cggatgaccc ataaagaatg aaagctagat gtattaggga
    15721 tcttgatctc taatacaaga actctcaaaa caatggctac cactccatca gtaaatgctt
    15781 ctaatctcga ctttgtcacg atgattcctc ctcagatgat tcagcgcttt tattttatca
    15841 cttacgttat taaataaccg tcgttttata taggaaattt tctgaaaatt gttgcatatt
    15901 tttcgaaa
//
LOCUS       DAXWOO010000054        15823 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.054, whole genome shotgun sequence.
ACCESSION   DAXWOO010000054 DAXWOO010000000
VERSION     DAXWOO010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.054"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            51..803
                     /locus_tag="ACGE70_001353"
     CDS             51..803
                     /locus_tag="ACGE70_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFF8693000.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            800..1714
                     /gene="pfkB"
                     /locus_tag="ACGE70_001354"
     CDS             800..1714
                     /gene="pfkB"
                     /locus_tag="ACGE70_001354"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFF8693001.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            1732..3663
                     /locus_tag="ACGE70_001355"
     CDS             1732..3663
                     /locus_tag="ACGE70_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFF8693002.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(3774..5333)
                     /locus_tag="ACGE70_001356"
     CDS             complement(3774..5333)
                     /locus_tag="ACGE70_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFF8693003.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            5488..7722
                     /gene="pcrA"
                     /locus_tag="ACGE70_001357"
     CDS             5488..7722
                     /gene="pcrA"
                     /locus_tag="ACGE70_001357"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFF8693004.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            7739..9775
                     /gene="ligA"
                     /locus_tag="ACGE70_001358"
     CDS             7739..9775
                     /gene="ligA"
                     /locus_tag="ACGE70_001358"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFF8693005.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            9881..10186
                     /gene="gatC"
                     /locus_tag="ACGE70_001359"
     CDS             9881..10186
                     /gene="gatC"
                     /locus_tag="ACGE70_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFF8693006.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            10186..11658
                     /gene="gatA"
                     /locus_tag="ACGE70_001360"
     CDS             10186..11658
                     /gene="gatA"
                     /locus_tag="ACGE70_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFF8693007.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            11655..13085
                     /gene="gatB"
                     /locus_tag="ACGE70_001361"
     CDS             11655..13085
                     /gene="gatB"
                     /locus_tag="ACGE70_001361"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFF8693008.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            13095..14150
                     /locus_tag="ACGE70_001362"
     CDS             13095..14150
                     /locus_tag="ACGE70_001362"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFF8693009.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            14261..15634
                     /gene="rlmD"
                     /locus_tag="ACGE70_001363"
     CDS             14261..15634
                     /gene="rlmD"
                     /locus_tag="ACGE70_001363"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFF8693010.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            15637..15765
                     /locus_tag="ACGE70_001364"
     CDS             15637..15765
                     /locus_tag="ACGE70_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFF8693011.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
BASE COUNT         5384 a         2899 c         3474 g         4066 t
ORIGIN      
        1 tgcgagtaga aaaaaaatat gctattattt gtattgaggt gatcgtgaaa atgcttacag
       61 aggaacgtag acaaaaaatc ctagacttgc ttgaacagca gaaaatcgtg aaatcacaag
      121 atcttgtaaa cctttttaat gcttctgaat caacgataag acgtgattta caagagctgg
      181 agaatagtgg gtttctagaa agagttcatg gcggagctaa gaaagagcaa ttattgaatt
      241 ttgagcagaa tatgagtgaa aaatcactca aaaacgttca cgaaaaacag caaatcgcca
      301 aatcggcagc tgaaaccatt aatgacgaag atgtcattta tttagacgct ggctctacta
      361 cgttagaaat gattcctttt ctgcaaggaa aacagatcac agtagtgaca aattccgttc
      421 aacatgcggc aaatttagtt gatttggagc ttgctaccat tattttaggt ggaacgataa
      481 agctggcaac taatgctgtt ttaggatcta atgcgataga acaactaaaa aattttcatt
      541 ttaataaagc atttatggga atgaacggtg cccatcttga acaaggattc acgactccgg
      601 acccagaaga ggcagctgtt aaacgtttag ccatgaaaaa ttccgaagag acatttgtat
      661 tgattgatca taccaaattc aaccagttgt ccttcacctc tgttgcacct ttggaagcag
      721 ccacgatcat aaccgagcgt tgtccattag aattcgttaa tgaattccgt gaaaagacaa
      781 caattaagga ggcaatacaa tgatttacac agtaacactt aatccatcaa ttgattatat
      841 cgtacgcgta gatgggttaa aactaggcga tttgaaccga atgaaggaag acttcaagtt
      901 acctggagga aaagggatca atgtttcccg tattttgaaa cggatcgatt ctcaatctac
      961 agcactaggg tttttaggag ggttcactgg cgaatttatt tcgaagtggc ttgaaaaaga
     1021 acaaatccag tctttgttca caaccgtaaa agaagataca agaatcaata tcaaactaaa
     1081 atcagatact gaaactgaaa ttaacggttt gggcccatct atttcagatg aagaaatcga
     1141 tgagttgaag caagcattag gaacagtagg acaaggagat atcgtcgtat tgtcaggaag
     1201 cgcgccggct agcctgcgaa aaggatttta tcaagaactg attgatatca ttcgagaaaa
     1261 aggtgcagat ttcgtgattg atacaacagg tgatgaccta aaaaatgcac ttgttaagaa
     1321 accattgttg atcaaaccaa acaatcacga attagctgag ctttacaata caaccttcca
     1381 atctgtagaa gaaatcattc cgtatggtaa aaaattgcta gctgaagggg cacagaatgt
     1441 gctgatctca atggctggag acggtgcttt gctcttcacg gaacaaggaa cttataaaac
     1501 gaatgtatta gttcgcccat tgaaaaattc agtaggtgca ggggattcaa tgattgccgg
     1561 ctttattggg gcttacagcc gtacgaaaga tccaatcgaa gcctttaaat ggggagttgc
     1621 ttgcggaagc gcaacagctt tttctgatga cttggcagct cgcgaattga ttgatgagtt
     1681 gctgccagaa gttgaaatca caaaaattga ttagtttcaa ggaggataat catgaatatc
     1741 aaggatttaa tggtcaaaga agcgatgatc atggatttgc aagcgactga taaaaaaggc
     1801 gcaatcgatg aaatggtcca aaaaatgttt gatgccggcc ggatttccga tatcgaaaca
     1861 tataaagaag gaattctagc ccgcgaagca caaacttcta caggtcttgg tgatggaatc
     1921 gccatgcctc acgcgaaaaa cagtgcagtt aaagaagcaa cagttttatt cgcaaaaagc
     1981 caaaaaggag tagattatga agccttagac ggacagccaa cttatctgtt tttcatgatc
     2041 gcagctccag aaggcgcaaa cgacacacat ttgcaagcat tagcagcact atctcgattg
     2101 ctgattgatg cagatttcgt aggaaaatta aaagaagcaa atacaccaga agaagtccaa
     2161 gacttgttcc aaacagctga agagcaaaaa gaagcagaag aaaaagcaga acaagaaaca
     2221 caagctgcag aagcttctgc tcatccagaa agaaaattcg ttgttgccgt tacagcttgt
     2281 ccaacaggga tcgctcacac ttacatggca gaagatgctc tgaagaaaaa agcgaaagaa
     2341 atgggtgtgg agatcaaagt cgaaacgaac ggttcagaag gaatcaaaaa ccgattgact
     2401 gctgaagaca tcgcccgtgc agaaggtgta atcgttgcag cagacaaaaa agtagagatg
     2461 aatcgctttg acggaaaaga actgatcaat cgtcctgtca gtgatggtat ccgtaaacca
     2521 gaagagctga tcaatctagc ccttagtggt actgcaccag tattccatgg tgatggaaaa
     2581 gaaagcagtt cagaagaagg aaatgcggat ggtactatcg gacaaagaat ttacaaagat
     2641 ttgatgaacg gcgtatcaca catgttacct ttcgttattg gtggaggaat cgctattgcg
     2701 ctttctttca tgatcgacca atttattggc gtgccacatg atcaattatc aaatctaggg
     2761 aattacaatc aagctgctag ctggttcaac caaatcggac aagctgcatt tggtttcatg
     2821 ctgccggtat tagcaggatt tattgcttca agtatcggtg accgccctgg tttgatcgtt
     2881 ggatttgctg ctggtgcatt agctaataca ggcggtgcag gattcttagg tgcactgatt
     2941 ggtggattct tagctggtta tgtcatcgta tttttacgta aattattcaa gaacttacca
     3001 aaatcattgg atggaattaa aacgatcctc ttttatccag tgtttggctt actgattact
     3061 ggtttcttga tgttgctggt caatgtccct atgaaagcaa tcaatgatgc attgaacggt
     3121 ttcttgacaa gtatgagtgg ttcgaatgct gctttacttg gtgcattatt agctggaatg
     3181 atggcggcag atttaggcgg tccaatcaac aaagccgctt atgtgtttgg tacagcaact
     3241 ttagctacga ctgttgcaac tggcggaagt gtcgtgatgg cttctgttat ggctggagga
     3301 atggtacctc cattggcaat tttcgttgct acacgtttgt ttaagaataa attcagcaaa
     3361 acagatcaag atgctggttt gacgaacatc gtgatgggac tatcgtttgt cacggaagga
     3421 tcgattccat ttgcagcagc tgacccgatt cgtgccattc caagttttat tattggttct
     3481 gctttaactg gcggattagt cggagctttc gggatcaaat tactggctcc tcatggcggg
     3541 attttcgttg tattcttact gagtcacccg gtcatgtatt tggtgtttat tgctatcgga
     3601 gcagtcgtat ctggtgtgat ttatggttta ttgagaaaag ctcctgcagc agcagaagca
     3661 tagaaataaa aaagaagagg ctggtttttc ccagtctctt ctttttttat agaatcatat
     3721 agaatcaagt agtgtagatg aatagttgtt gtaaaatagt ttttccatgt tcgttaaaat
     3781 gattgcttca aatttgtgat agaagattta gcctcgattt cctgcttgat tcttggcaag
     3841 tttgcttcat cagtcaagat aaccaaaata tctcctacat tcatgatcgt atcaccgtga
     3901 gtcaagattt cctgatttcc tctgcgtata gagattagca gcatattttt aggccaattg
     3961 aagtctcgta ccattaatcc gtctaaagaa ctctctgccg ttactggaaa atcaaaagtt
     4021 gtccttgcac cttttagtga ggcggtctga gtatttccaa gtagacgttc caataatgat
     4081 tcgtaaattg gattaccgcc gagaaggtca ttgacgatat aagcaacaag agcagccaat
     4141 cctaaaggca tcagatgatt aagagagcca accatttctg tcactaaaat aattgctgtc
     4201 agcggtgctt ttccaatcgc tgtgaaataa ccagccatgg caataacgac aaaatccttg
     4261 atgtagattg gatccatccc gaaatcaacc ataatgtttc cataaaagct accgattaaa
     4321 gcccccaatg tcaatatcgg aaggaaaatc ccccctggaa gaccaccacc gtaagaaatc
     4381 atggaaaaaa cgaatcgaag aacaaacaaa ccgataatca taatgattgg caaggaagca
     4441 tgtccaaaag ctaaaacgat ttgattgcct ccgcctagag tactaggcca taaataacca
     4501 attggcaaaa tcagcaagaa aggaacgaac ccgtgaaaat aagctggtaa aggcaatcgg
     4561 ttgtaaaatt ttggcaaagc cagcaagacc ttctgataaa taaaacctaa gatgcctaga
     4621 agtactccta agatcactaa ggaaccataa tatttcaacg gcaaagaagg aaggtcacct
     4681 agatataaaa caggtctcaa gccgaaaaag taaagagaga cgaagttagc agtgattgct
     4741 gaagcaaatg atgtcagcca aacaagtgga gaaaaggagt gatggatttc ttctaaaaca
     4801 aagagtaatc ctgcgatagg agcattgaat gctgctgcaa gtccgctact tgcaccgcta
     4861 gaaattaaaa tcttttcttc tgaagatgga ctccgaaaca attgtccagc tccttgtcca
     4921 atggatgcgc caagctgtat agaaggtcct tctctaccta gaaaaaggcc cgcaccaact
     4981 gaaagtacac cgccaatgaa ctttttccat aaaacagaga accagttcat tgataattcc
     5041 ccgcgaagct gtccctctac ttgtggaatc ccgcttccct tgatatccgg ttcatttttg
     5101 accagataac ccacgaaaaa tgctgcaagg atgcagcaaa tagctaggcc aattaagtaa
     5161 atcggctgtt cccgactcat ttcatagata cttcgaacta aatcgcttag cttttcgata
     5221 gataatcgaa acaggctaac gacgattcct gtcagcagtc caacaaatat tccttttaga
     5281 ataaaatata tctgagtctt atctaatttc ttgatttttc cttttttttc cattcaaacg
     5341 cctcgctttc ttttaattta gcgcgaaatc aacgaattga caaggaaaaa tccataaaaa
     5401 gaaacaaatg attttgaact agctagttta atgaaagcca agaaaatgct ataattaaca
     5461 agattgataa aagaaaggat gttgttaatg tcatccaaag ctgaattatt aaacgggatg
     5521 aacccccgtc agaaagaagc tgttcttcac actgacggac cattactgct aatggcaggt
     5581 gcaggaagtg gaaagacgag agtgctgaca caccgcattg cctatttgat tgaggaaaaa
     5641 gaagtcaatc cttggaacat cctagcgatt acttttacca ataaggctgc aaaagaaatg
     5701 aaagaacgtg tcaatgcgat tctcgcatct ggaggcgaag atgtctgggt atcgactttt
     5761 cactcgatgt gtgtgcgtat tttaagaaga gatgttgatt ttatcgggta taaccggaac
     5821 tttacaatca tcgattcttc tgaacagctg accttgatga aacggatact aaaagaactg
     5881 aacattgatc cgaaaaaata tgatcctcga agtatattag gaacaatctc tcaagcaaaa
     5941 aacagcctgc agacccctca ggattttgca aaaatgcaag gcagttatta cgaagagatt
     6001 gcggcaaaat gttatgctgc ttaccaaaaa gagcttcaat ataatcaatg tatggacttt
     6061 gacgacctga tcatgaatac gattcgatta tttgaagaac atcctgattc actcacatat
     6121 tatcaaaaca aattccatta tattcatgta gatgagtacc aagatacaaa tcacgcacag
     6181 tatacgctag tcaatttatt ggcaggtaga tttaggaacc tgtgcgtcgt gggagatgca
     6241 gatcaaagta tttacggctg gcgcggtgca gatatgcaga acatcttgga ttttgaaaaa
     6301 gattatccag atgctgcagt aattttgcta gaacaaaact atcgctccac gaaaaacatc
     6361 cttagcgcag caaaccaagt cattgaaaac aatagcaatc gaaaaccaaa aaacttgtgg
     6421 acagaaaaca aagaaggaaa caagatcact tactaccgtg cagataatga acgagacgaa
     6481 acccgtttta tcgtagatcg gatgcaagaa gagattcgaa gcaatcatcg gaattacggc
     6541 gactttgcca tcttgtaccg gacgaacgca caatctcgtg taatggaaga aactctgttg
     6601 aaagcaaata ttccttataa aatggttgga gggcacaagt tctacgatcg taaagaaatc
     6661 aaagatattt tagcgtattt gaatgtgctg gcaaatcctc aagattcaat cagttttgag
     6721 cgtatcgtta attcgcccaa acgaggcatc ggaccaggat ctatcgaaaa actacgcagt
     6781 tttgcttctc ttcatgaatg gccactgctt gaagcagcgc aaaatgtgga tctggcaaat
     6841 atcggtggaa aagcaggaca gcaattaggt gcatttggag agatgatcca agaagtcact
     6901 cagatgatcc catacttgac agtgacagaa ttgacgaaag aggttttgga tcgtagcgga
     6961 tatctagaag acttaaaaat ccaaaataca ttagaagcac aagcacgcat cgaaaacttg
     7021 gaggaatttt taacggtcac acaagaattc gacaaacaat tcgaacagca aaatgaagaa
     7081 gacgctgatg caccagaaga aaaactaacg gtcttcttaa atgatctggc acttgtttcc
     7141 gatattgaca atttagaaga agatgcttcc caagtgacat tgatgacttt gcatgcagcc
     7201 aaaggattag aattcccagt tgtattcctg attggtctag aagagggggt ttttccttta
     7261 tctcgagcat tgatggaaga aagcgaactg gaagaagaac gccgtctcgc atatgtgggg
     7321 atcacgcgag cagaagaagc gttgtattta acaaatgctt tctccagaac attatatgga
     7381 cggacgcaat ataaccgacc ttctcgtttt gtagaagaaa tcgatcagga gttactggaa
     7441 atcgaaggca tgcgtcctac gcctaaaaaa acacctgtat tcgctaaaaa aacagcatat
     7501 agctataaac agccagagac agcggttgtt ccctctaaaa gtgctactgg aggggaaaat
     7561 aacagctgga aacctggaga taaagtcaaa cacaaaaaat ggggtctagg aacagtcgtc
     7621 cgagtaagcg ggacgtcgaa ggatctagaa ctagatgtgg ctttcccaag tcaaggagta
     7681 aaacgtcttt tggctgcctt tgcgccaatt gaaaaagctt agtagtggag ggtaaatgat
     7741 ggaacatgaa atgacgattg acgaagctgc taaacgagca gaagaacttc gtacaagact
     7801 gaaccaatgg tctcgcgaat actacgtaga agataaacca acagtggaag actatgttta
     7861 tgataaagaa tacgcagaat tagtagcaat tgaggaacaa tatcctgatt tgatcacgtc
     7921 tgattctccc actcaacgag taggcggaaa agtattagaa ggttttgaaa aagtcactca
     7981 tgatattccg ctttacagtt tgaacgacgt tttcagcaaa gaagagctga tagcttttga
     8041 tcaacgtgta caaaaagcag tgggacgtgt agttgactat tgctgtgaac taaaaataga
     8101 tggactttcc gtttctttgc gttatgaaga tggaaatttc gtgagaggtg cgactcgcgg
     8161 ggatggaact gtaggagaaa acatcactga aaacctaaaa actgtccggt cggtcccaat
     8221 caaattaaaa gaaccaatga acatcgaagt aagaggcgaa tgtttcatgc caaaacggtc
     8281 atttgtccaa ttgaaccaag acagagaagc agagggaaaa gatatctttg cgaatcctcg
     8341 gaacgccgca gctggttctt tgcgtcagtt agattctaaa atcacagcga aaagaaatct
     8401 ggacactttt ttatacacag tagctgattt cggtcctatg caagcaaaaa cccagtatga
     8461 tgcgctagaa gagctagaaa aaattggatt ccacacgaat cgggaaaaac gtctatgtca
     8521 ttcgattgat gaagtgtggt catacattga agaatatcat gacaaacgtg tggatttgcc
     8581 ctatgaaatc gatggaatcg tcatcaaagt gaatgaattc tcgctacaag atcaacttgg
     8641 ttttactgtc aaagcaccga gatgggcagc agcttacaag tttccgccag aagaagttga
     8701 aacgctaatc gaaaacatcg aatggacagt cggtcgaaca ggggtagtaa cgccaacagc
     8761 tatcatgaca ccagtccgag tagcaggaac gacagtaagc cgtgccagcc tgcataacgg
     8821 tgattatatt aagttgaagg atatccgctt gaaagataca gttctgattt ataaagcggg
     8881 agacatcatc ccagaagttt cgcaagtcgt gttagataaa cgacctaaag acagcgaaga
     8941 ataccaattg cctacacatt gtccagtttg tggaagtgaa cttgtccatt tggatgaaga
     9001 agtagcctta cggtgcatca atcctaaatg ccctgctcaa atgaaagaag gtttgaacca
     9061 ctttgtttca agaaatgcga tgaatatcga tgggttgggg ccgcgcgtac tagaacagat
     9121 gtatgataaa aaactagtag cagatgttgc tgatctttac aaattgacag aagaagagtt
     9181 attgacgttg gacaaaataa aagaaaaatc agcaaataat attttgacag caatcgacaa
     9241 cagcaaagat aattcggtcg agcggttgat tttcgggtta ggaatccgac atgtcggagc
     9301 gaaagcagca aaaattttag cagaacattt cggtgatcta gaaacattga gtaaaagtga
     9361 ctatgagtcg attattgcgc ttgatacgat cggtgatatc attgccgaca gtgtagtcac
     9421 ttatttcagt aacgaagaag tccatgaatt gatgaatgaa ttaaaacaag caggcgtcaa
     9481 tttcgaatac aaagggcttc gaaatgccca attgcaagaa gtcgagtctc cttttaaaga
     9541 aaaaacagta gtccttactg gtaaactaac acgatttact cgtgaagaag caaaagagac
     9601 gatcgaaaat cttggcggga aagtgactgg tagcgtatcg aaaaaaacgg atatcgtggt
     9661 agcaggagaa gatgctggca gcaagctgac caaagcacaa gaattaggaa tcgaagtttg
     9721 gacagaagat caaatggcag atgcattggc taaaagtcgc tcagtagaag aatgaaaaat
     9781 gagttttttc agacaaattt caataaacaa tttcagacac ggcactcttg tgttaaaata
     9841 gagagcaaag tttttaactc gtttagaaag aaggggtagt atggcgatta gtgaagaaca
     9901 agtgaaacac gttgccaaat tagcaaaact tgcgttcgca gatgaagaac ttgcaagttt
     9961 taccgaccaa ttaggtaaaa tcatcgatat ggttgaaatg ctggaagaag tagatacaga
    10021 aggtgttcca tttacgtcga atgtggttga aacagtaaac gtgatgcgac aagatcgggc
    10081 gcaagctgga tggaaccgcg atgaattatt gaaaaatgtg ccggaaacag aagacggctt
    10141 tatcaaagta cccgcaatca tcgataatgg ggaggctggc gcataatgac agaattacac
    10201 aatcgttctc tagaagaact gcatcatatg cttgtatcga aagaaatcac tgtacaagat
    10261 ttgacaaaag caacatttga acgaatcaaa gaaacagagc ctgaggtcga agcattcatt
    10321 acattgaacg aagaaaaagc attggaacag gcaaaagctt tagatgaaaa agggattgat
    10381 gaaagcaacg tccttgctgg tattccaatt ggtatcaagg ataatatcgt aacaaaagat
    10441 atcttgacaa ccgctgcatc taagatcctt tacaattttg aaccaattta tgatgcaacg
    10501 gtcatggata aaatctatca agcagatatg atccctgttg gtaagctaaa catggatgaa
    10561 tttgctatgg gtggaagtac agaaacttct tatttcaaga aaacaaaaaa tgcttgggac
    10621 ccaacaaaag tacctggtgg ctcttctggt ggttctgcag cagcagttgc agctggacaa
    10681 attccagttt ctttaggtag tgatactggg ggaagtatcc gccaaccagc agcttttaac
    10741 ggcatcgtag ggatgaaacc tacttatggc cgtgtctcac gttttggttt gattgccttt
    10801 gcttcatctc ttgaccaaat cggaccaatg acccgtacgg tgaaagataa tgcattagca
    10861 ttgacagcta tcagcggtta tgacgaaaaa gacggtactt cttcaggcgt ttcagttcct
    10921 gattttgcag agggactgac aggcgacatc aaaggaatga agattgcttt acctaaagaa
    10981 tacttaggcg aaggtgtggc ccctggcgta aaagaagctg tattgaaagc agcagaaaca
    11041 ttccgttcat taggtgctgt tgtggaagaa gtcagcttgc ctcattcaaa atacggtgtg
    11101 gctgtttatt atatcattgc ctcatcagaa gcaagttcca acttgcaacg attcgatggt
    11161 atccgctacg gttatcgctc agaaaatgtg aaatcattag atgatgtata tgtcaactca
    11221 cgttcagaag gattcggtga agaagtaaaa cgccgtatca tgttaggtac attctcctta
    11281 agtgctggtt attatgacgc ttacttcaaa aaagcgggac aagtccgtac attgatcaaa
    11341 cgagattttg aaaaagtctt tgcagattat gacttgatca tcgggccatc ttcaccaaca
    11401 gttgcattcg gaatcggtga gaacattaac gatccaatca caatgtacat gagtgacatc
    11461 ttaacgatcc ctgtcaactt ggctggactt ccaggtatgt ctgtaccggc aggtttatca
    11521 gaaggattac cagttggatt gcagatcatc ggtaattatt ttgatgaaaa aacgatgtat
    11581 caagcggcat atgcgtttga acaagcaaca gaattccata aacaacaacc agctatttta
    11641 ggagggaaag acgaatgaat tttgaaacag tcattggact agaagtccac gtcgagttaa
    11701 aaaccaactc aaaaattttc tctccagcgc cagctcattt tggtgcagaa ccaaacagca
    11761 atacaaacgt gatcgactgg ggttatccag gcgtgctgcc agtaatgaat aaagctgcgc
    11821 tggaattcgg gatgaaagca gctttggcat taaactgtga aatctctaaa gatactcatt
    11881 ttgaccggaa aaattacttt tacccagata atccaaaagc atatcaaatt tcccaatttg
    11941 atcaaccaat cggtcatgac ggatggatcg aaatcgaagt agaaggtaaa aagaaaaaaa
    12001 tccggatcga acgtgtccat ttagaagaag atgctggaaa aaacatgcat ggtattggcg
    12061 gttattctta cgttgatttg aaccgacaag gcacgccttt gatcgaaatc gtttctgaag
    12121 ctgatatgcg ttcaccagaa gaagcatacg cttatctaga agcattgcga tcaattatcc
    12181 aatttacaga agtcagcgat gtgaaaatgg aagaaggctc tatgcgctgt gatgccaata
    12241 tttctcttcg cccttatgga caagaagagt tcggtacgaa agcagaattg aaaaacttga
    12301 actctttatc tttcgtgaaa aaaggtttag cttttgaaga aaaacgtcaa gcaaaagttc
    12361 ttctttcagg aggagaaatc caacaagaaa cacgccgttt tgacgaaaca acgaataaaa
    12421 cagttttgat gcgtgtcaaa gaagggtcta gtgattatcg ttacttccca gagcctgata
    12481 tcccgaaatt cgtgatcgat gacgaatgga ttcaaaaagt aaaagctagt ttgcctgaga
    12541 tgccagcttc tcgtcgcgaa cgctatatcc gtgagtttgg acttccagag tatgacgcaa
    12601 tggtattgac acttacaaaa gaaatgtctg atttttttga agctgttttg gaaaatggtg
    12661 cagatgccaa gcaagcatct aactggttga tgggtgaagt ttctgcttac ttgaacagtg
    12721 agaaactgga attagctgag acaaaattga cacctgagaa cttagcagga atgattcgtt
    12781 tgatcgccga tggaacgatc agctcaaaaa tcgccaaaaa agtcttccgt gaattgatca
    12841 caaatggcgg agaagcccaa gaagtagttg aaaaaaatgg ttgggtgcaa ttatctgatc
    12901 cgaacaaact actgccaatc attaatgaaa ttttggataa caaccaacaa tctgtcgatg
    12961 atttcaaaaa tggaaaagat cgtgcggttg gcttcctagt cggtcaaatc atgaaagcaa
    13021 ctaaaggaca agcaaatcct ggtgtcgtga ataagctact taaagatgaa ttagcgaaac
    13081 gatagaggct gaaaatgaaa aaagcacgcg taatttataa ccccacctcg ggaaaagaat
    13141 tattgaaaaa aaatttggcg gatatcctac aggcattaga agaagcagga tttgaagcca
    13201 gcgcctatgc aactacacca gaaccagatt cagccaagaa tgaagcatgt agagtcgcag
    13261 aactaggttt tgatctagta gtggcagctg gtggcgatgg cacaatcaac gaagtggtta
    13321 atggaatcgc tccgcttgaa caccgtccta aaatggccat catcccagct ggtacaacga
    13381 acgactatgc tcgagcatta aaaattccta gagataatat taaagcagca gcagaagtga
    13441 taaaaaaaca ccaaaccgta aaaatggaca tcggcctttc tgataacagt tacttcatca
    13501 atatcgctgc aggtggctat ttgacagaat tgacttatga agttccatca gaactaaaga
    13561 gtatttttgg ttatcttgct tatcttgcga aaggagcaga gatgctccct cgagtaaagc
    13621 caatcaaaat gcgcttgaag tatgatgaag gggaatatat cggaaatgct tcgatgtttt
    13681 tcctagggct gacgaactct gttggaggat tcgaacagat cgcgccagat gccaaattgg
    13741 atgatgggaa gttttcttta atcattgtga agacagcaaa tatctttgag atcttgcata
    13801 ttgcagcact catgctgaat ggcggtcgac atgtcgatga tccaagaata atctatacca
    13861 aaacaagcag tttgttcgtt gaaacagaag atgatatcaa tcgaccagta atgatcaact
    13921 tagatggtga atacggcggg gaagcaccga tgcatttcca aaatcttcat caacacattg
    13981 agttctttgc caataccgat cagattcctg atgaagcaat cacaggtaca gatgaagaag
    14041 aattagaggt tgtcagcaaa gaattcgtaa aagaagtcga acgtttaacg gatgaagata
    14101 tcgacggtga cggaaaaata gcagacgaaa cacctaatca aaagaattaa tcaaaagccg
    14161 gtgctgaacg tagtaaggtc agtgccggtt ttagttgtgt aattagctga tttatagtag
    14221 agttcagtag gattgaaaaa taggcttaaa ggagcaaaat atgacagaac agcaaatagt
    14281 aaagaaaaac gaacgatata cagtcgaaat cgtcgacttg agttatgaag gaatgggggt
    14341 agcaaaaatc gatggttatc cattatttat cgaaaatgca ctccctggag aagaagtaga
    14401 aatactagta gtaaaagcag gcaataagtt tggatatgga aaagtcgaaa catttattac
    14461 acaatcacct gatcgtcagc ctgtagaaaa caatgcatta cttcgcatag gtatcgcacc
    14521 tttagcccat ttgaagtatg aacaacagct tgtcttcaaa caaaaacaag tagagcatgt
    14581 aatggcgaag attgctaaga tgccagaagt tcctgtactt ccaacggtcg gaatggcaga
    14641 accattcggc taccgtaata aagcacagat ccctgttcgc cgtatcgatg gacagttagc
    14701 caccggtttt tacaaaaaga atagccatga tctagtccct atcgaagatt tttatatcca
    14761 agatcctgtc atcgatcaag caatcaaagt agtcagagat atcttgcaac ggttccaagt
    14821 acgaggatat aatgaagaaa aaaatgaagg acagcttcgt cacatcatta tccgtcgtgg
    14881 tcattattcc catgaaatga tggtcgtttt agttactcgt aaagaaaaat tctttaaatc
    14941 caaggaaatt gctgaagcta ttcatgaaga attgccagaa gtggtttcgg tcatccaaaa
    15001 tatcaatgaa gaaaaaacaa atgtgatttt aggagacaaa gaaaaagttt tatatggcag
    15061 agactatatc gaagatcagc tattaggaaa aacctatcga atctcagcaa agtcatttta
    15121 tcaagtaaat actcaacaag cagagtatct gtaccaaaca gccatcgagt ttgccgagtt
    15181 agataaaaaa gacattgtga tcgacgctta ttcaggaata ggtacgattg gcttgtcttt
    15241 agcagacaga gtggataagg tgtatgggat ggaagttatt ccagaagcaa ttgaagatgc
    15301 acagttcaat gctttaaata ataaaatcga aaatgttcat tatgaagtcg gaaaagcaga
    15361 aaaagttatg aaaaaatggc aagaaaaagg gatcaaacca tctgtgatcg tagttgaccc
    15421 tccacgaaaa ggattagatc atcattttat tgaatcagca atcgaaatgt caccagaacg
    15481 aatcgtctat atctcatgta atccggcaac ttttgcaaga gatgctaagt tatttgcaga
    15541 agcaggttat gccgtgaaga aagttcagcc tgtagatctt ttcccgcaaa cccatcatat
    15601 cgaattagtc gcttcattcg ttaaaggaga ataaaaatgg accaaaaaga aaaaatgaaa
    15661 gaattgatca aaaagaaaca aggtggaggc cgatccaaac aaatggaaac acctaagaat
    15721 gatcgtaaaa acatgagaaa aggccctaaa atctttaata aatagacgtg ctaaagaaag
    15781 actaagaagt gttaactttt gttattgttt cttagtcttt tta
//
LOCUS       DAXWOO010000055        15620 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.055, whole genome shotgun sequence.
ACCESSION   DAXWOO010000055 DAXWOO010000000
VERSION     DAXWOO010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15620
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.055"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            62..3229
                     /locus_tag="ACGE70_001365"
     CDS             62..3229
                     /locus_tag="ACGE70_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFF8693012.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            3297..3794
                     /locus_tag="ACGE70_001366"
     CDS             3297..3794
                     /locus_tag="ACGE70_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HFF8693013.1"
                     /translation="MILLLGFILWQTPVHASEFNFAVDFELPANQIDKNKTYINVRME
                     PGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESKSKFDETLSYDMKNLAK
                     TVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKENVSNKSTVGVVNQFSQV
                     KAILI"
     gene            3813..4277
                     /locus_tag="ACGE70_001367"
                     /pseudo
     CDS             3813..4277
                     /locus_tag="ACGE70_001367"
                     /inference="COORDINATES: protein motif:HMM:NF023225.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
     gene            4274..5032
                     /locus_tag="ACGE70_001368"
     CDS             4274..5032
                     /locus_tag="ACGE70_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFF8693014.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(5118..5858)
                     /locus_tag="ACGE70_001369"
     CDS             complement(5118..5858)
                     /locus_tag="ACGE70_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFF8693015.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            5919..7172
                     /locus_tag="ACGE70_001370"
     CDS             5919..7172
                     /locus_tag="ACGE70_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFF8693016.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            7494..7721
                     /locus_tag="ACGE70_001371"
     CDS             7494..7721
                     /locus_tag="ACGE70_001371"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFF8693017.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            7844..8170
                     /locus_tag="ACGE70_001372"
     CDS             7844..8170
                     /locus_tag="ACGE70_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFF8693018.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            8238..8951
                     /locus_tag="ACGE70_001373"
     CDS             8238..8951
                     /locus_tag="ACGE70_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFF8693019.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            9261..10112
                     /gene="licT"
                     /locus_tag="ACGE70_001374"
     CDS             9261..10112
                     /gene="licT"
                     /locus_tag="ACGE70_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFF8693020.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            10233..12098
                     /locus_tag="ACGE70_001375"
     CDS             10233..12098
                     /locus_tag="ACGE70_001375"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFF8693021.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            12112..13555
                     /locus_tag="ACGE70_001376"
                     /pseudo
     CDS             12112..13555
                     /locus_tag="ACGE70_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            13663..14364
                     /locus_tag="ACGE70_001377"
     CDS             13663..14364
                     /locus_tag="ACGE70_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFF8693022.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            14490..15002
                     /locus_tag="ACGE70_001378"
                     /pseudo
     CDS             14490..15002
                     /locus_tag="ACGE70_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(15400..15609)
                     /locus_tag="ACGE70_001379"
     CDS             complement(15400..15609)
                     /locus_tag="ACGE70_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit beta"
                     /protein_id="HFF8693023.1"
                     /translation="MYYFKQQILVSFINEKIILFSCYVSLINDVSLNKKADELNDQNL
                     IIFVHQLLLKIFLLTINSWICKNIT"
BASE COUNT         5223 a         2649 c         3380 g         4368 t
ORIGIN      
        1 attggaaata gattaaaacg ataaaataaa attgtcaaaa cgtcagagag gatggtggag
       61 aatgaatcaa tgtgtaaaaa agctgattgc gtcgattttg tttgtcttcc ctctcttgat
      121 ggtgacatcc gtttttgccg atgtagagaa aacaaacgaa tcaacgcaat cagagaatca
      181 aagttttgac aagactactt tttcagaaga atcgattact tctgaaacta cagaatctca
      241 aagtgaggct tctactaata aaaggcaaga agaaaataag acaagtgaga cctcagaagt
      301 agctgaacaa acgtctgata gcagtgaagc ggcactggca agagctgttt cagcggatgg
      361 gtacagcgaa gctgccacaa cagaagaact ggcgcagctt ctcgcagacg aatcagtaac
      421 taagatccga ctgattcagc ctttgacttt agacagggaa ttagagatca aacgagatat
      481 cgttattgat tttggtggat tcgctcacaa tttcggcaca caccatattt atatcaatga
      541 gatacctcag tcgatcgagt tccagaattt taaaggaaca gctgcacatc caggtggatt
      601 gacaccaaat atcgatggaa atgccattat cattgcttat atgaccgatt tttggggaag
      661 cagatatcat tttacgggag aaataaaatt taccgggacg cttgatttgt atgatgggtc
      721 caaattagga ctgatttacg ctcctcgtgc aactgtcaca ttagatggtg tttccggtgt
      781 gttggatgtc caaccagtga aggaaggact gaatgccgct ccgggtaaag cctatttttg
      841 tagaagctat caattgaatg tgatcaatgg tagtcaactt tatggaccgt atctagggaa
      901 attctacggt tttctagatt caggagatga gactggtagt ggaaaaacag cgccaggaat
      961 ccatatctta agcggttcaa aggtctctct tgattatgat cgggcagatg gcgttagcga
     1021 tgaaggagag gcagtcgata cgctacctgg taatgtcatg tttgaagtca gtggttcggg
     1081 aagcgaattt tcggtaaata caaagatcaa tattaccaat gacaacaatc gcggaatcat
     1141 ccaaatgaga ggttcaggat cgaaagtcac tgtatcgaat ggtgggaaaa tcaccatcaa
     1201 tacagcaact acgggcggat ttcgcttgca aggagattct agtcaaatcc atgtatcaag
     1261 tggtggacaa atctcggtca aaatggctgg agatgacgat caacccggga acaatgggat
     1321 gcgttttgta ggaaaaggcc tttctttgat cgtagaagga gccggatcag ttatcgacat
     1381 cgataagcag agtgggcgtt ccagcgcagt tcgttttgag aatggtacgc aaaagctgac
     1441 agtaaccaat ggaggctcat tgagggtaag aaatacaggt gatggcaaat cttacgtgaa
     1501 cagactcaat caaggaaatc aagcgatcca attttatgac gcttctggtt ttctagaaag
     1561 tccaggttca gctgatttta cagtcactgg agaaaaatca aatatcgata tacgcgcaga
     1621 ttccggagca actgtagata caactggaga tattgattta aattttacgg caggtgaaaa
     1681 aacttattta gtcatggtcg ggaaaacagg ggaagataag accggcatca tcagtgggga
     1741 tgtgttgaat gtggacttag aatctcctac ctattttgat ttccaaaaca aacggattgg
     1801 aagcgagacc aatggcatag gcggctgggt attccaagga aatggagatt cttctttaaa
     1861 gatcgcccat tcagaaatcg ctttatggcg aaaaacaggc gcaacattcg ataactttga
     1921 aaaagacccg gattattttt ctgatatgac agacttatcg atcaatggga ccgacttagg
     1981 caatttcgta tcaagcccta gtgcaggttt aacaaatgaa tttgctcaga caggtgccgg
     2041 aatgaaaaat tataatcgaa tcagtgccaa taatcagctc ccgatgattg aatccttaag
     2101 agtgccaacg aatgcagata aaaaaatcta tgggcatgtc gttgttcccg aaggtgtcga
     2161 ggcaaagcca agagatgctt ggacaggcga agtagaagtg actttacgta ttacttacgc
     2221 agatacgacg aaatcatctg tagaaattac tgctctcacg caaggacaaa cagatgaaaa
     2281 tgaaggggca ggatataacc catggggaga aggcgaacaa ggcggcttgt tcgaagttca
     2341 agtacccaat gatgagagat taaaaacgga ggatcaagtg caagttgttg ctgctaaaaa
     2401 agtaagaggt ggccaagaag tgacagaact ggactctgcc aaaacgacag tcgatgtgat
     2461 tcctcctgaa cctgctaatt taagtaccac attgatcaat gcagcttctc gcacgatcac
     2521 aggtacaggt gaagcaggag ccttggtttc attgaaaaaa aatcaagctg ttttgtcaga
     2581 aacaactatt gatgatacag gggaatttgt tctttctatc ccagaaaatc aactttttgc
     2641 aggagatgta ttagaaattg tattaaacga ccaagcagga gaagctactg ctaagggtgt
     2701 gcagaacaag ccaagtacca acgatgaaat tgggaatcac aatccaagtg atacgccagt
     2761 tgtttatcat gatgccccgc cttttgcacc tgctacgaag atcgtagtag aaggtggtct
     2821 ctcatttctt gccccttcca aactcgattt tggtcagatc aaagcaactg gtttgacaca
     2881 agaagcattt ggccaaatgg atatacagga aaaattatgg atttatgatc agcgagaaac
     2941 gaaaaatccg tggatcttaa cgataaaatt atctcagcct ttcacaaatg atgcaggatt
     3001 ttctatgcag gatgtcctat atttcaagaa aggagaggag tatcaattag tgaatgaaga
     3061 agaactggaa attgtcagag gacaaaattc aacgaatgtg caagcagatt attcagaata
     3121 tttaaataac ggtcgggcgt ttaaacttgt cttaaccaaa gaaaatcaga tagctggtaa
     3181 ttactcagca gagataacat ggacattagc agatgcgccg acagaataaa gatcaaggag
     3241 gatgattgat gaatcgaata tggaataaaa gtcatcgaaa caactgctct ttacttatga
     3301 tccttctcct tggattcatc ctttggcaga cacctgtcca tgcctccgaa ttcaattttg
     3361 cggtagattt tgaattaccg gctaatcaga ttgataagaa caaaacatat ataaatgtaa
     3421 ggatggagcc tgggaaaaca caggaaatcc cctacacggt ctttaaccga acagataaat
     3481 cagtaacagt tgagacattg atccactccg ctacgacgaa ttcaaacggt gtgatcgaat
     3541 atggagaatc aaaaagtaaa tttgatgaaa cattaagtta tgatatgaaa aacttagcaa
     3601 aaacagtggg agaaataact gttcctcctc aaagtgcggt cactgagaca ctttcactga
     3661 aagcgccaga aaaagagctg gctggtgtgg tcgcaggagg gatcacgttt caagaaaaaa
     3721 aagagaacgt ttctaacaaa agtacagttg gtgtcgtcaa tcagttttct caagtgaaag
     3781 caatcttgat ttgaaatagc gaaaaagagt cttccccaga tttgaaatta aaaaaagctt
     3841 atgcggatca aagcgatgga agaaatgtga ttaaggcgaa tcttcaaaat ccaaatgctt
     3901 cttatatgtt cggcttgact acatcggttg aaatctacaa aaaagaaaca aaagagcctt
     3961 acctttcaat aaaaaaagaa aaaatagatg tagcacccaa tacaaatttt gatttacttg
     4021 tcccattaga aggaaaaaaa ttagctccgg gacattatca agcggttgtg aagggtgctt
     4081 ggaaagaaaa acgctggaat ctccataccg attttgaaat aactgatgat gcagcggacc
     4141 aattgaatga aaaggatatt gatttaaaaa atcagccaga aacatttgac tggcgatggc
     4201 tgatacttgt tggagtcatt cttattggct tatttgctgg attatttatt ttaaaagaaa
     4261 gaaggaaaaa gaaatgaaaa aaacaattgc attaagtacc gttctagctg gaagtctatt
     4321 attatgtaca caaagtgttt ttgctgagaa taagagctca gccactacag aaggaacgat
     4381 ttctgtgacc aaaccagatg ctgcaaatcc tgaagacgtt gttccagttc ctcctgttga
     4441 tccaaatgat cctgatactc cttttacacc tgacgaacca aatcagggaa catccggatt
     4501 actgacgatt gaccaagctc cagatcttga ttttggaaca atcaagttag gtacagcaaa
     4561 aacagtatat gctgcattac aaagcggaac ggacagtact ggagcacaaa cacaagtaca
     4621 gaatttcgta caagtaacgg ataaaagcgg aaattatgcc ggttggaaat tatcagtcaa
     4681 acgaacagaa tttattggta caacatctga tacgaataaa ctaactggtt caaaggtgaa
     4741 attcaaaaac gccgtgattc gaaatagcgc aactaatagc agtggaatgc catctaccgt
     4801 ttctggaact ggcgctgaag gaatcgaaat cccagcagat caggcagtag atctagtaac
     4861 tgcaggagca aatgaaggga ttggcacatg gatttattca ttaggagaga atgtggaaca
     4921 agggagacaa tcagtggaat tatttgttcc tgtatccaat tatgctgtag acaactacgt
     4981 ttcaacattc acatggtcgt taacagatgc tcctacagaa gaggaagcgt aaaatcatat
     5041 caacgatttc agggagtgga gccgattcaa ggcccactcc ctgaaatctg ctatgcttac
     5101 gtctcagctc ttattcatca actcgaagca gttttttgga agtcatggaa acgttccttt
     5161 gtcttttcaa agatctttcg accaacgagt aaggagtcac cgttgaaaaa ttctaacaca
     5221 taatgagcag aatcaatttt ctgcaattgc gataagttga tacagtaaga ttttaaaagt
     5281 aaaaagtaag catacttttc taactctgtc ttcaattgtt ttaaagaagt attcactaat
     5341 ttatcttctg tagttgtttt gaccataact tggtttttca aacctttgat actttcaata
     5401 tataaaattt cccgaggata caggacgaga cttccacctt tttgatcaat aaagatttta
     5461 tcgacattta cgatctgctg ttctttccaa ttgatctgtt ctagcacttg acgaatttgt
     5521 tcagataaat tcctgtcttt tctgatatat ccaatcggaa ggattttccg attgatgatc
     5581 tctactgcaa aattcgtata agcggtgaaa aaaacaatat tactatgtgc atttttttta
     5641 cgtatttgtt cggcgacatc aatacctgtg agtgattgct tcaagtcgat atcgataaag
     5701 aaaacatcgt gattatcaat agtgcccaac tggatcaact gattgatttg agagatagaa
     5761 gtgagctgca cgatattctc gatatgatga ttgaccaatt ctctgatgat ccattcgctg
     5821 tgaaactggt tatcttctat gataaaatac tttttcatat gcaggctcct attaaaaaaa
     5881 tatgtgtaca ttttgaaaca aaacaatgga ggaataagat ggtcgcaaca gtgacacttt
     5941 atttgacctt tactttgatt ttagatttag gtatctattt attcttttcg aaacaactgc
     6001 tagtaaaaaa cagctttggt tgtatagctt tgatggtggt gacactttta cttcctatcc
     6061 ctaaagtgag ctttccttac ctagttggct tattattgat gtttcttttt tattttacta
     6121 cgaaaaacct gtttatcagc tgtttgatca gtaatctttt ttttgctatc actggactca
     6181 gcttttttct ggtttttgat ctgatggatt atttaggaaa gagacatctt ggaacagcaa
     6241 gtttttttgc actacttatc gcatttattt tctatctatt agtaaaaagt atagacaaac
     6301 agacgagatt tctcaacttg ctttttactt atccaactga acactatttt ggaggtattt
     6361 gtattttatt aagctggggc gtgatttact tggtattggg aagtgttcac ttccgctcat
     6421 ctaattatct gcttctatct cttttgtcga tagtcatcag ttttttttct ttgttttttt
     6481 ccattatgtt gatcagtttc caagtaaaag aaagacaaca tcaggaatcg cttcaactgt
     6541 atcaaataaa tgaagaatat taccatcatc tagaggaatt caaacatgat tataaaagtc
     6601 tactgttttc attaaaaatt actcttcagg aaaataaaaa agaaacgttg gaatttttag
     6661 aaatattaga agatcattct gcagctattt ttgaaaaacc gcaaatacaa cagctaacaa
     6721 atattgtatc cccagctgtc aggggtgtct ttttaaaatt tctagaaaaa gcagaatcgg
     6781 agaaaatccc gatccaagta gttattcctg tcgaagtgaa aacaatcccg attgatctgg
     6841 ttgtttttat tcgctgttta tctattttta tttctaacgc attcgatcat cgtgtagccg
     6901 atcagagtcc gattacgatc aatctgctgg aagaaaaaga gggatcacgt tttagtatag
     6961 cgaacaaggc aaatccaacg aatttgacgc tatcggagat ggtcaaaaga ggaaaaacct
     7021 ctaaaaaaaa tggcgggtta ggtctataca gtgcgaaaaa aatgttggat gtttacgaaa
     7081 atgcagaact aaaaatcgaa ttttcaaaac agaatcacta tttttttgta gaaatgtatc
     7141 tggcatctag aaagagcaaa tcagcctact gacttactgt caaaggataa gtttgaggaa
     7201 agtctacacg gagatagaga caacaacaca gcatattcaa aagaaacaga agcgtgatga
     7261 ccgttgtcac ccttctgttt cttttgctat gtagttaaac aaagtagttg acacttccgc
     7321 tacttagtat tagtacgaaa gtatacttca tgatactaag tagataagaa gtgaaaagcg
     7381 gaacccacga tcgtcaaaat gctaactgct ttgcttacac cagataaagg gaagagcaat
     7441 atcgcttatt ttaggcgtgg agaagcaagc aaatccctac gatcacaaaa aaaatgctga
     7501 cccagcagtt aagagagtta gaacaagatg aaagtatcca tcgaaaagcc tataaccaag
     7561 tcccgcctaa agtagagtac tcgctcaccg aagaaggaaa atcattaaga gagattctag
     7621 tagcgttgtc tgtttgggaa gaagaacgta tcgaaaaaca aaaagcctca agggaagaag
     7681 acacgctact gcatacacat ggtggctacc taaattattg attttctatg acatttattt
     7741 taaataaaag aaaacaggag tttttttaaa agcatcatct attttttatg taaaaaaatg
     7801 gtatgattaa tcaagtaaga agtgattgga agaaggcaag caaatgaata gtatggaaaa
     7861 agtcaaccag tttagagatg aacgtaattg gcgacaattt cataacgaaa aagatttggc
     7921 tatctctatt tctttggaag catctgaatt gttagaactt tttcaatgga aaacgccgga
     7981 agaagtaaaa gaaacacaat tagaacgaat caaagaagaa ctggcagatg tattgatcta
     8041 ttcatacatg atggcagaca accttcacct ggatctagat gagatcattg aagaaaaatt
     8101 gatcaaaaat aatttgaaat atcctgtaga aaaaagcgca ggtgtacgaa aaaaatacac
     8161 agagttgtag aaatgtgtca ttcggctgtt ggaaagagca aaagaaaaac agtaaaatga
     8221 gtctggaggg agagaaaatg aaaaaaaaag gaaaagcaaa aagatggcta gtcaatatcc
     8281 tcttgttcct cttgttatta gtaggactag ctttgatttt caatgaacag atcaaggatt
     8341 attttgtcag agagacagga gataaatatg ctatagcgaa tgtaacaaaa gaagatctga
     8401 aaaagaataa cgataaagat gtcagttttg actttgatgc ggtagaacca atgacgacag
     8461 aaggggtcat gaggtcgcaa atgagaggaa cagatctacc agtgattgct agtatcgcag
     8521 tcccttcagt ttcaatcaat ttaccggttt tcaaaggctt ggataatacg tcactacttt
     8581 acggagcagg aacattatct ccagatcaag aaatgggaaa aggaaattat gccttagcaa
     8641 gccatcgtgc aacgaaccct gaattgctgt ttacaccgct agaaaattta gagatgggtg
     8701 ccaagatcta tttgacagat ttagaaaatg tgtatacata caaaacattt tttaaagaaa
     8761 aagttgctcc tacagatacc caattactga atgaagtcga gggaaaagaa attgtcacac
     8821 taattacttg tggcgacatg gacgccgtca cgcgactagt tgtacaagga gaactggaaa
     8881 gtgttacatc gataaaagat gcgacagatg atatgagatc tgctttcaat ctagagacaa
     8941 aaacatttta ggatactggg acataagtcg ttacagatta aaaacagcca aaggatatga
     9001 atcgataagg aaaaaccaat ttgctgaaaa aaagagaaac gcaaagaggt gcatctctga
     9061 tttggttgtt ttctagatag acttgtgttc cagttttttt attttttata tgaaaaggct
     9121 cgcataaata aaaattcgat ggtatgatga ttttgaataa gaaatggatt gttactgtac
     9181 ggcaggcaaa accagaattc agctaagaaa cagtaggcga attctaggtt tttttatttt
     9241 tcttgagagg gcgaagcaaa atgaaaattg ataaaatttt gaataacaat gtcgtcattt
     9301 cgaaaaatgg attcggggaa gaagtcgttt gtatgggaaa aggactagct tttcaaaaaa
     9361 agattgggga cgagatttcg ccagaagcgg tccaaaaaga atttgtttta agagattctt
     9421 ttgctaaaaa ccaattccag cagctgatgg ctgatattcc ggcagaggag atcgaatggg
     9481 tcaaacaagt agtcgaactg gctgaagata aactaaaagt tgaattttcg cctaatattt
     9541 atctgacatt gaccgatcat cttcattatg cgatcacacg agcaaaagaa aatatccaac
     9601 tgcctaatcc attattattt gaaacaaaaa aattttatcc gaaagaatat caagcagcga
     9661 aagaggctct tttgttcgtg aaacaaaaga cgggtgtgga actttctgag gatgaagccg
     9721 gttttattgc ctttcatttt gtgaatagcc aacaaggaaa cgaagacatg caagtgacga
     9781 tgaccgctac cacaatggta agagatatac tgagtatcat cagtaaattc ttcggcatcg
     9841 tgttcgatga agaatctttg aattatcagc gtatcattac tcatctgcag ttttttgccc
     9901 agcgttatct caaaggggaa ggatctgacg agcaagatga atttctctac gcattagttc
     9961 aaggaaagta tccaaaagcg tttcattgtg tcgaacggat caatgagtat cttcgacaaa
    10021 cgcaaaaaca ggaaatgggg atcgcagagc agatttattt aacgattcat attcaaagag
    10081 tagtaagtga gaaaaaaagc atccaatcat aaggattgtt actgtatggc aggcaaaacc
    10141 tggattgaaa aaataggttc acgaatgttt gtgactgttt ttttcaatct gggtttttat
    10201 tttttaaata aaaaagaaag aaggaaaaaa caatggataa tcaagcagtt ggtcgtcgtg
    10261 tatgggaagc ggtaggagga caaaagaatg tcaaaagttt agtacattgt gctacgcggt
    10321 tgcgattcag attaaaagat gaatctttag cagatacgca aaagttaaaa gaagacccgg
    10381 acgtgatcca agtcgtacaa agtggcggtc agtatcaagt agtgattggc agtaatgtag
    10441 cagatgttta tcaatcgatc gtggatgagg aaggtcttgc agtttcggag gatggaaaag
    10501 aagagtccaa aaatattttg aatcgtctga tcgatattat ctctagtata tttacacctt
    10561 ttctaggggc gatggcagcc gccggggtat tgaaaggatt cttatcattg gcaacggtga
    10621 tgggctggct gacagctgat agcggtactt atcaaatatt gtttgctgca gcagatggtg
    10681 tctttacctt cttacctgtc atgcttgctt ttacagcagc taaaaagttc aaagccaacc
    10741 agtttttagc agtagcaatt gctatggcac ttgtgtatcc agctatcaca gcagttgctg
    10801 gtgcgggaga agctatcagt ttctttggga tcccagtgat tctttcacca tctggttata
    10861 cttcttctgt cataccaatc attctagccg tatgggttca aagtaaatta gagccattcg
    10921 taaaaaaagt gattccgcaa tttctgcaaa tgattttagt tccgcttgtc gttttagtag
    10981 tgatggttcc attaacattt cttgctttag gaccaatcgg gacggttgcc ggtaatgctt
    11041 taggcggtct cttcaactcg atttatggat ttagtccaat cgtagcaggt ctaatcatgg
    11101 gaagtttatg gcaagtattc gtgatgttcg gcatgcattg gggattcgta ccgatcatgt
    11161 ttttgaatat cgaacaatat gggtttgatg tattgatgcc aatgcttctt ccagccatct
    11221 tggcacaagg tggggctgct ttagctgtag cattgcgaac aaaagatacg aaacttcgag
    11281 cattagggat ttcttctaca gtgacttcgt tatttggaat cactgaacca actgtgtatg
    11341 gtgtgacatt acctttgaag aaaccattca ttgccgcttg tatttctgga ggtattggtg
    11401 gagctattat cggattttcc ggagtaaaag ctttctccag cagtttggtc agcttattga
    11461 cgattccgac tttcattaat acggtggatg gtgtagagtc aaatgtgaca gtagcagtaa
    11521 tagctacagg aattgctttt gttcttgcat tcgtaggtac attgatttta ggatttgacg
    11581 aacaaacaca agacaatcaa ttagaaaaca aacatgcgaa tgcaggagaa ccgattactt
    11641 cagcacggca taccttaaaa agtcctttaa cgggtaaagt actgccattg agtgaagtac
    11701 cagaccaagt tttttcttct ggcgtgatgg gaaaaggtat cgctattgat ccagaagtag
    11761 gagaactagt tgcacctgca gacggagaga tcacgactat ttttccaact ggacatgctg
    11821 tagggatcac aacgactgac ggagcggaga ttttgattca tatcgggatg gatacagtag
    11881 aattaaatgg gaatggcttt gagatcttag tcaaacaagg ggatctcgtc aaagcaggag
    11941 atttattgat tcgttttgac atcgaagcaa ttagagcagc tggctatagc gtaatcactc
    12001 cagttgtcat tacgaatact gatgcatttg ccgatatact tgaattagac caaaaagaga
    12061 ttattgcaaa cgaagatgtt ctggcaattg taaaataagg aggaaatcag gatgcaatca
    12121 cgtttttcag aaaaattttt atggggtgga gcaactgctg ccaaatcaat tagaaggggc
    12181 ttatgatcga gacggaaaag gactatccgt tgcagatgct atgcctggag gtaagcagcg
    12241 ctttgcgatt atcggcagtg aagaatttga ctggacgatt gatcaagaaa aatatattta
    12301 cccgaatcat cgaggaatcg atcattacga tcgtttcaaa gaagatcttg ccttgtttgc
    12361 ggaaatggga ttcaaatgtt atcgtttttc gattgcctgg acacgaattt ttccaaatgg
    12421 agacgaaggt actcctaacg aagcgggatt agaattttat gatcagctga ttgatgagtg
    12481 cttaaaatat gatattgaac ctgtgatcac gatttctcat tacgaaatgc ctcttcattt
    12541 ggcaaaagaa tacggtggct ggaaaaatcg aaaactgatc gacttctatg aacgatttgc
    12601 tcagaaagta ttagagagat acagcagtaa agtcaaatat tggatgactt tcaatgaaat
    12661 caattcggcc tttcactttc cagctttaag tcaaggatta gtaaaaagca atggagcagg
    12721 agagtaccaa aatattttcc aagcatggca taatcaattc gttgcaagta gcaaagccgt
    12781 aaaaatcggc cacgaattgc gttcggatat ccaaatcggc tgcatgatta tctatgcaac
    12841 aacttacagc atcgatgcca atccagtcaa tcaggcagca acaatgatcc aaaaccaaga
    12901 attcaatttt ttctgcacgg atgtccaagt acgtggagaa taccctgcat acacagcgcg
    12961 tacccataaa aaatatggag tcgatccgga aaaattagag caaacggaaa aagacttcca
    13021 gttgctaaaa gaatatccag tggactacat cggatttagt tattatatgt ctacggcaat
    13081 caatgagact gatccacaag cagcaacttc agaagggaac ttgctgggag gagtaaaaaa
    13141 tccgtttctt gaggcaagtg aatggggctg gcagatcgat ccagaagggc tgagaatcgc
    13201 attgaatgaa ttatacaatc gttaccagaa accattattc attgttgaaa acggtcttgg
    13261 agccattgat catatggaag aggatggcac gattattgat gattatcgga tcgattattt
    13321 acgtcgtcat atcgaagcga tggctaatgc agtagcagac ggtgtagatt tgatgggcta
    13381 tacgccttgg ggatgtatcg atcttgtcag tgcatcaaca ggagaaatga gtaaacgtta
    13441 cggctttatc tatgtggatc tagacgatga aggaaatggg acactgaatc gatcaaagaa
    13501 aaaatctttt gattggtata aagaagtaat tgcctcaaat ggcgtaaact tgtaattaaa
    13561 gcagctgaat gaaaaagaac acacttctca catgtgattt gtgttctttt tttatttttt
    13621 atgtgctatt cttttagtaa tagtaaagca aagggtgacg ttatgaaaag gacacgtgta
    13681 ctatacttgg aagtcgcaga taaaattaaa gaagatattt tctcaggaaa atatccagtc
    13741 ggttcgatgt tgccgacaga aagtgaatta gaagaactgt ttcaagtcag caaaatcact
    13801 gtccgaaagg caatcgaact gctggcatca gatgaatatg tcgcaaagaa aagtggtcgt
    13861 gggacgacgg ttctaagcaa tcgtccttac aatcgattat ccaaagcggc aagctttacg
    13921 caaatcttgg agaactcccg atatgaagtc cggaaagaaa cgctggattt gcaattggtc
    13981 catataaaga aggaagatcc tttgttttcc tttttcggtg agcaagctgt ttgttttaag
    14041 cgcttatatt atttgaacaa tcagccttat atttattttg ttcattatct tccacaagaa
    14101 atgatgcaat tcagcagaga agaatttggc aaagaatctt tgtatcgttt gctgacgaaa
    14161 aaaggatata ctattgactc attttctgat gatttctcgg cagtattttt aacagaagaa
    14221 gaacaaggaa ttctgaaaac agacgcacag atcggcatca aacggatacg gaaatcgtct
    14281 acgcaaattg gggaaatgat tgaatattcg gaggcggtct accatacgga gctgcatcct
    14341 taccatattg attatgaaac ataaataaaa aaagaggttg tgaaaaagct gccctgcatc
    14401 aagtaataag aacagccaaa caaaaatagc ttctcatatt tttcgtttgg ttaggcttat
    14461 taccgcagat acacggctgt ttctggatta ccatttattc tggataaggg ggtgtggcac
    14521 gattttcaga aggaatctat gaaccaatca ctgtattgat tttagcttcg atggtaaccc
    14581 cattcggctt gacgatcgct tatttcttag ggaaaatcat tcgtaaaaat atactcaatc
    14641 gtcaagaaat cgatacattg aagaccgctt ttcctatggg gatctgtcag atcacagagg
    14701 gatgtttccc tatcgtattg aacgacttgg cacgcaatgt catagcaacg ggagtcggtg
    14761 ggacagtagg tggcggatta agcatgtttt ggggcgcaga tagccgtatt ccggcatcgg
    14821 gaatgtttgc agtcgctacg atgaccagac catgggcatt cattggtgca ttgttagctg
    14881 gttcttttgt cacaggaata accattttat tattgaaaaa accagtagat ccgaatgcag
    14941 aaatcatcca agaagaaaaa gaggaagaag acatatcttg ggatgatttg acaattagtt
    15001 aggagaggca gaatggatta caagaaaaaa gtacaataga tgtaccttga gacaggaatg
    15061 gtatgaaaga atcagagtta gaaagaatcg aatatacaga ttttggttta ggagcgatcg
    15121 aaactgtcca gagcatcggc accctccttg aaatgacgat gaaggaaaaa tgagaacctt
    15181 tcgttgtcga aagggtctgg tataccttat gtcgaaggaa aggaaacaaa aaaactttcc
    15241 tgtactattc caaaaaggaa agaaagttat tatacagtaa aaaaagaaat tattcttcat
    15301 gctagagatc agtacacgtt cgagcccaat atcaaacact agttccaggc aggcgcaaca
    15361 ggtgcggtta tcactgaatt ttcttccagc agtgacgatt caagtgatat ttttacaaat
    15421 ccagctattt atcgttaaca aaaagatttt taggagaagc tgatgaacaa aaatgatcaa
    15481 attttgatcg tttagttcgt cggctttttt atttagagaa acatcattta tcagtgaaac
    15541 ataacacgag aacaaaatga ttttttcgtt aataaaagaa acaagtattt gttgtttaaa
    15601 ataatacacg ttattggcat
//
LOCUS       DAXWOO010000056        15583 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.056, whole genome shotgun sequence.
ACCESSION   DAXWOO010000056 DAXWOO010000000
VERSION     DAXWOO010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.056"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(77..796)
                     /locus_tag="ACGE70_001380"
     CDS             complement(77..796)
                     /locus_tag="ACGE70_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8693024.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(927..2078)
                     /locus_tag="ACGE70_001381"
     CDS             complement(927..2078)
                     /locus_tag="ACGE70_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFF8693025.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKTTGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTYSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDVGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(2295..3035)
                     /locus_tag="ACGE70_001382"
     CDS             complement(2295..3035)
                     /locus_tag="ACGE70_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFF8693026.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEIFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(3039..3395)
                     /gene="rsfS"
                     /locus_tag="ACGE70_001383"
     CDS             complement(3039..3395)
                     /gene="rsfS"
                     /locus_tag="ACGE70_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFF8693027.1"
                     /translation="MIDSKEQLKIAVEAADSKRAEDIIALDIRNVSLLADYFMICSAN
                     SERQINAITEEIIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERSFYNLE
                     KLWSDAPLVDLSEWLD"
     gene            complement(3419..4012)
                     /gene="yqeK"
                     /locus_tag="ACGE70_001384"
     CDS             complement(3419..4012)
                     /gene="yqeK"
                     /locus_tag="ACGE70_001384"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFF8693028.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDVEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(3996..4646)
                     /locus_tag="ACGE70_001385"
     CDS             complement(3996..4646)
                     /locus_tag="ACGE70_001385"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFF8693029.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(4656..4967)
                     /gene="yhbY"
                     /locus_tag="ACGE70_001386"
     CDS             complement(4656..4967)
                     /gene="yhbY"
                     /locus_tag="ACGE70_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFF8693030.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(4983..6092)
                     /gene="yqeH"
                     /locus_tag="ACGE70_001387"
     CDS             complement(4983..6092)
                     /gene="yqeH"
                     /locus_tag="ACGE70_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFF8693031.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(6089..6616)
                     /locus_tag="ACGE70_001388"
     CDS             complement(6089..6616)
                     /locus_tag="ACGE70_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFF8693032.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(6843..7628)
                     /locus_tag="ACGE70_001389"
     CDS             complement(6843..7628)
                     /locus_tag="ACGE70_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFF8693033.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRFDQHELLEKRYKRFRQY"
     gene            complement(7621..8490)
                     /gene="accD"
                     /locus_tag="ACGE70_001390"
     CDS             complement(7621..8490)
                     /gene="accD"
                     /locus_tag="ACGE70_001390"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFF8693034.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(8491..9870)
                     /locus_tag="ACGE70_001391"
     CDS             complement(8491..9870)
                     /locus_tag="ACGE70_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFF8693035.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDHF
                     GNVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(9878..10297)
                     /gene="fabZ"
                     /locus_tag="ACGE70_001392"
     CDS             complement(9878..10297)
                     /gene="fabZ"
                     /locus_tag="ACGE70_001392"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFF8693036.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(10322..10798)
                     /gene="accB"
                     /locus_tag="ACGE70_001393"
     CDS             complement(10322..10798)
                     /gene="accB"
                     /locus_tag="ACGE70_001393"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFF8693037.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPETMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(10801..12036)
                     /gene="fabF"
                     /locus_tag="ACGE70_001394"
     CDS             complement(10801..12036)
                     /gene="fabF"
                     /locus_tag="ACGE70_001394"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFF8693038.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIIGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(12055..12792)
                     /gene="fabG"
                     /locus_tag="ACGE70_001395"
     CDS             complement(12055..12792)
                     /gene="fabG"
                     /locus_tag="ACGE70_001395"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFF8693039.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(12804..13721)
                     /gene="fabD"
                     /locus_tag="ACGE70_001396"
     CDS             complement(12804..13721)
                     /gene="fabD"
                     /locus_tag="ACGE70_001396"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFF8693040.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(13828..14052)
                     /locus_tag="ACGE70_001397"
     CDS             complement(13828..14052)
                     /locus_tag="ACGE70_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFF8693041.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(14105..15067)
                     /locus_tag="ACGE70_001398"
     CDS             complement(14105..15067)
                     /locus_tag="ACGE70_001398"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFF8693042.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(15077..15517)
                     /locus_tag="ACGE70_001399"
     CDS             complement(15077..15517)
                     /locus_tag="ACGE70_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFF8693043.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         4082 a         3557 c         2679 g         5265 t
ORIGIN      
        1 aaaagcaaga caatctgtta gtgagattgt cttgcttttc gaaaaagact gaatactttt
       61 cgttaaaact ggttgcttag tcttctaatg ccacattatg gaacacatgc tgtacatctt
      121 ctagatcttc cagtgcatca atcatttttt caaatgtttc ttgatcttct ggagatagtg
      181 ttacctcgtt ttgagggatc atttggatct cagcgattga aaaatcagtg attccttttt
      241 cttttaatgc ttcttgtata ctatggaaat ccgctacttc accgtaaacg atgatttgtc
      301 cttcttcttc cattacatcg cgaacatcga tgtctttttc cattaggtat tcaaggatct
      361 cgtcagcgtc ttctcctgca aaagcaaaga ttgctgtatt gtcaaacatg taagaaaccg
      421 ctccggaaac acccatgttc ccgccatttt tcccaaatgc tgcccgtacg tctgctgctg
      481 ttcgattcac attgttcgtc aatgtatcaa caatcaccat tgagccgctt ggtccaaatc
      541 cttcatagcg caattcagag tatgtttcat ctcctgcacc ttttgctttt tcgattgctc
      601 ggtcaataat atgttttggt acattataag ttttagcgcg atcaatgacg aaacgtagtt
      661 tttggtttgc atgcggatca ggatcacctg attttgctgc cgcgtagatt tcaatcccaa
      721 attttgcata aatccggcta ttattagcat ctttagcagt tttctttgct acgatatttg
      781 cccatttacg tcccatattt tcacttcgct ttctgatttg atagtttcat aacgcaaaaa
      841 cgtcctgttc cattatactt gtgaatcacg attctgtgaa gctttttttc tttcttcctt
      901 cattcttctc atttttagtc aattatctat actcggattg ggatttgccc gaaattctgt
      961 tccggaatat tgcctcccaa ttgatagatt tgatcacttc taactaacag ttccgcttct
     1021 tgtgcctctt tttttccgat tcgggaataa atagaaatct cttttttttc ttttaagaat
     1081 cttcttccaa catcggtaaa tccaagtagt cgaagataat ttttattttg ttggacagga
     1141 atctcttgct ttttgatatt cagcaagaca taacaagata atcgttggat ccgtgtccat
     1201 gtatatcttt ttgtttttac ctttgacaca aaatcctcaa aactatcagc agttgcagct
     1261 gctgttttca tgcgcgattc tattccttcg gtcatctgat agatatctgt cagttccagc
     1321 aaagtagaag ataacaaccg ataacgcaaa tacggccaat aagattccca agtctgcagc
     1381 gaataagttg aaagatcttc ttctgtctga ggcggcacaa gtccagctat tctttttcct
     1441 tcttctaacg catggcgaat ggctgttgca ctggctatcc tctgatctgt gatgattttt
     1501 tcatgatagc ctgcactttt acgtcggatc ggatgtaatt ccatagggtg gacatacttg
     1561 gcattttcct ttgcataact taatcccaag atatgatttg gcgaagaaaa atcaagcgta
     1621 atatcgggat acagactgcg aaaaacctca gtcatttttt gcggataact atacgtttga
     1681 tcagacagtt cttgaaatgc ctcttctatc ctatcttgat tctcttggac aaagcgtccg
     1741 aaactgctgt aatcaaacga agtctcttct tctgttccaa aacacaaact gtcacagtcc
     1801 agtgtttgca agatttttac tgcaccttta gcaaaatagt cagctgactg aaccgcccat
     1861 tcgacgggaa gctccactac taaatccaca ccattttgta aagcttcatt cgcgcgatgc
     1921 catttatcga tgatagccgg ttcgcctcgt tgcaaaaaat tcccgctcat tacagcaatg
     1981 acgacctctg ctcctgttgt ttttcgtgcc atttccacat gatagcgatg tccattgtga
     2041 aaaggattgt actctacgat aatcccgcag cttttcatgc gcatcctcct tcatagttat
     2101 cgataaaaaa gaaatagaga caagtaaaaa aaggcaaaaa tcggaaaaaa tacccgaact
     2161 atataaacta ttctcgcaaa cacaatactt ctgcgacgaa aagctgcact gcaagagcaa
     2221 tgcctatatc gttcggatat ataatttatg ttgaagattt attcttttct tgttaactcg
     2281 agcaggcaaa accgctagtc tttgtatgct acaaagaacc agcgctttga tttctctgat
     2341 gctgcttcgt ctgtaaaatc agaaaatgct tcgactttcg aaaagccgct gttttccagc
     2401 attcttaaat aactttccaa tggataggta cgctcttgat gtagttcatc ttcacgaata
     2461 aatcgaccag acccttcttg ttcttcaacg aaaaaggtaa gaaaatgttc gatgctgtgt
     2521 ttctcgcttc ctggataact atcccacaaa aaagcaaaat tatccccttg ataatgatag
     2581 ctatattctg gaaaaatttc atctacttga taggtggagt gtacgtcaaa taaaaatcgg
     2641 ccattttctt ctaacgcttg atagacttca tcaaacactt gctgtacctc ttgtctattt
     2701 ttcatatagc acagcgaatc agaaaaacag gtaattgcct gatattgtcc gatttctgat
     2761 aaatcgagca tattgccttc cacaaattgg atcgatgcgt cttcttcata tgctcgctgg
     2821 ctggcaatca tcaacatctc ttctgaaagg tctaaccctg tcactttata tccatctttt
     2881 gcaaaggcaa ctgctaatgc tccagtaccg catgccagtt ccaaaatatt ttttgtgtct
     2941 tcttgtaaat gacgtttcga aaaagctagc cattgctgat aaagtgtttc gtccatcact
     3001 tcatcgtaaa tgaaagcaaa tgtttcataa gccatatgtt aatccaacca ttcactaaga
     3061 tcgactagcg gagcatctga ccatagtttt tccagattat agaaacttcg ttctggtgca
     3121 tggaaaacat gtacgatcac atctccaaga tcgatcaata cccattttcc gccttctttt
     3181 ccttcaatac gttttacttc atatttgttt tcttcttctt tatcaatgat ttcttctgta
     3241 attgcgttga tttgacgttc gctatttgct gaacagatca tgaaataatc tgccaacaag
     3301 gaaacatttc gaatgtctaa agcgataatg tcttctgccc gttttgaatc tgctgcttca
     3361 accgcaattt ttaattgttc tttactatct atgatgattt cctcctaata tgagcagctt
     3421 atttttttgc tacccagtaa ttatatgttt cgattgtttt tggataaatc tgttgttctt
     3481 gcgcaagcaa atgggccaat gtatgttttg tttcaaatgc tacagcttcg tctagatcag
     3541 cccatgcaat ggctcgtgca tcttgtaccc caggaaaatc tcgacctggt tcaatatagt
     3601 ctgctacata aatgattttg tcaaggagac tcatcttagc agccccagtc gtatgcaccc
     3661 ggattgcatg aagaatttct acatcggtga ttcctaattc ccgctctaca aacgaagcgc
     3721 caacaagacc atgccagatt gcattattat aattcaataa ctctggatca aaaccgtctc
     3781 ttacgattac cattttgaac tcctcatctg gtcgttcttt tgcataatca tgtgtcaatg
     3841 cagcaatact agctttttca ggagaagcac cgtatttttt ggcaagagcc acggctgttt
     3901 cttctactcc aagcacatgc ttgaagcgtt gttcgctcat tctcatctgt attttttgca
     3961 tcaggttttc tctagaataa gcagtatatt cattactata gtccatattc atacaaccct
     4021 tcattttgaa tataatcaat tactttatct ggaacaaggt agcggataga acagccttct
     4081 ttgatctttt gacgtatttt cgtggaactg atgtcgatct caggtacatc tacccaaatt
     4141 acaggatatg gagtatccgt tgtgtatcct ggtcgacgta tacccacaaa gttgaccata
     4201 gaagtcagct catcgatttt ataccatttt ggcaaatatt ctaccatatc cccaccgata
     4261 ataaagtaat aatctgtatc cggattgttt tgcgtcaatt ccttcatagt atcataggtg
     4321 taacttttcc caccacgagc taattcaatc gtttcaatct gcaaaaaagg attgtcttcc
     4381 acagctaatt ccaacatgtt gagtcgatgt tttgcatcaa tcgtctgttt ctcatcaaca
     4441 tgaggtggct gataagaagg catcaaaaat acgcggtcaa gacctaaatt ccggccagct
     4501 tgttcagcca tcactaaatg tgcaaggtga acgggattga atgttccgcc caagatccct
     4561 acttgctttt tcttctctaa gaattgaggc atttcttcag gaaataattg tgaacgaaca
     4621 aatgtcttag cttgcgtatt catcatggtc actccttata ttgcttttac ttcttttgat
     4681 agcctttgat acttttcttt tgtggaaggt ttgtataata caataacacg tccaatgatt
     4741 tggacgattt cgcaatctat ttctttttct aatacttgag ctgcttcttc tgcaatctca
     4801 tctgtatttt gaagtaacga tactttgatc agttctcttt tttctaaagc ttcttcaatt
     4861 tgaacgatca ttgctgaatt gatcccacct tttccgattt gaaaaatcgg ctgaagatga
     4921 tgcgctttgc tacgtaaaaa acgcttttgt tttcctctta aactcataat tttccttctt
     4981 ccttaaatca atgctttgcg tttcaataca tctacaccct ttggcgccca gcccgcaatt
     5041 actactggcg ctgttacagt gatccagcct agaccggcaa aaacgatgtc tgttttttct
     5101 ttcacagaaa actcaaaacg aacaagctcc ggaaatgcct cgacttcatc aggacgagga
     5161 ggctgtaata atccacctac atgtttttga taaaattcgt ctgctttttc cagtttcgtt
     5221 cgatgaatat ttacatcatt tgacacataa gcaataaatg aagcacgatt gccgctgata
     5281 aaatcaaatc ttgcaagtcc gcccaagaaa agtgtttgcc cttcattcag ctgataaact
     5341 ttaggtttga tttcttttaa cggtgcaatg atccgcaaat cttttttacc taagtagtgc
     5401 gccatttgat ggcgatggat aattcccggt gtatcaatca aaaaatgccc gtcatctaaa
     5461 ggaatctcga ttttatccaa ggtcgttcct ggaaactgcg aagttgtgat tacatcttgt
     5521 actcccgctg tatttttgat gatttgattg ataagcgtcg attttccgac atttgttact
     5581 ccgacgatat agacatcccg tccatcgcgg tattcttcga ttttttctaa tagttccgtc
     5641 atctcatgag atttcttagc actagttaat aatacatcta ctggacgaat tcctgcttcg
     5701 taagcacgtt ccttcatcca ttgtgttaat ttagtttttt tcaatgattt aggtaatata
     5761 tccactttgt tcccaactaa aagtactgga ttgtcaccga caaaacggtg tagtcctggg
     5821 attagtgaac cattgaagtc aaaaatatcc accacattga cgatcaatgc atctttctgc
     5881 cctaattcat tcagtaagcg caaaaagtcg tcatccgtca atgagacatc ttggatctca
     5941 ttataatggc gtaatctgaa acaacgctga caatagactt ccccggaagc taaacctttt
     6001 tccaaagccg tttgcggcgt gtatcctaat tcaccgggtt tgtttgtttg tataacggca
     6061 ccacagccga tacattgtaa ttcttctgtc attcgattcc acctcgccag cccatatcag
     6121 ggtgttttgc taatagatag gccataattc ttttttccca aaaacgatta aattgtgtct
     6181 tccaactatc tgttgcgata atgggtttta caaggatgct acgtatccct gcacgattgg
     6241 ctccacgtat atcagtcata atctgatcgc ctaccattac tatctcatca tctgctaaat
     6301 ccaattgttt ctttgctatg ttgatccctc ttgctaaagg tttcattgct cgtgcaacat
     6361 acaaaagatc aaatttttca atcgctcgcg cgacacgttt tgaattattg tttgaaacaa
     6421 caacaacagg gattcctgca tttttcattt ctaaaatcca agtaagcaac tcttctgttc
     6481 catctggatt attccatgca atcaacgtat tgtctaaatc ggtcaatact gctttgatgc
     6541 ctagattctt taattgggca ggtgtaatct gatagatggc ttctaccatc catgttggtt
     6601 tgtaatttga aaacatgctg tctcctcatc tgcaaaaaat gaaggagcac ataaagtact
     6661 cctcaacccc gtattatact gcatatcagc aaaaaaaact acctctatac tcgttcgtct
     6721 ttcagaaaaa gcatttcttt acaaaatgac caaaaataaa gattgatcac agaaaaattt
     6781 agaaacaaaa aacaactaag cgagcaagac cattctttac tctttgctta gttgttaaac
     6841 acctaatatt ggcggaacct cttgtatctt ttttccagta attcatgctg gtcgaatcga
     6901 acaagttccg tcaatctttc aatcaacgct tttttcataa tgtgtgcgac tttatatcgt
     6961 ggcaactgct cgccgttgaa cgtttcagga ataactcgat caacgattcc taactcgcgt
     7021 agttctggcg cagtaatttt catcaactca gctgcttctg cggcgcgact accgtctttc
     7081 cacaaaatcg acgcaaatcc ttctggagaa agcacagcat aaatcgtttc ttccagcatc
     7141 cagacttcat ccgctaaacc tagagcaagg gcaccgccac ttccaccttc accaatgatg
     7201 atggcgatga tcggtacctt taaatcagac atttcgagca gatttctggc aatagcctct
     7261 ccttcgccgc gctcttctgc tcctacaccg caatatgcac ctgctgtatt gatcaatgta
     7321 atcaccggtc tattgaactt ttccgcttgt ttcatcagtc gtaaagcttt tcgatagcct
     7381 tcaggatggg gagaaccaaa atttctgcgg atattttcct gcagatcccg tcctttttga
     7441 ataccgatca ctgttaccgg tttgccatct aatcgagcga ttccaccaac aatcgcttcg
     7501 tcatctgcaa acaaacgatc accatggcat tcctcgaaag aatcgaagat ctcttcaaaa
     7561 aaatccaagc ttgtccatcg ttctttagct cttgctaact gaacgacatc atgtgctgtc
     7621 ttatccacga tctatccatc ctttctgtgt atgcaattca agaagcgtat ggattttctg
     7681 cttcaattcc atgcggggaa caatctgatc gacaaaacca tgagataata agaattcagc
     7741 cttttggaaa tcttctggaa gttcctgctt gatcgtctgt tcgatgacgc gtcttccagc
     7801 aaaaccgatc aagctttggg gttctgctag aatgatatcg ccttccattg caaaactggc
     7861 agttactcct ccggtcgtcg ggtccgttaa aacagttaga taaaaaagac cttcattgct
     7921 atgccgtttg acagcagcag agatttttgc catctgcatt agagaaaaga ttccttcttg
     7981 catccgtgcg ccacctgaag ctgtgaacaa gacgactgga agactttcct ttgttgctcg
     8041 ttcgaataat ctggtgatct tttctccgac aatcgttccc atacttccca tgataaaatt
     8101 aggatccatc actccaataa tgaagggaat cccttgaatg gttgctttac cagtgaggac
     8161 tgcttcatga agtcctgttt tatcttgcat ttttttaatt ttttccgtat attcgggaaa
     8221 tgctagcgga tctttggtta caagatccgt atcccattct tcaaaacttt tttcatcaat
     8281 aatcaaagct agacgctgcc atgcaccgat tcgaaaagcg tatccgcaat gagggcatac
     8341 tttttcttca ccgatgtctt tcgtataaag gatatgtttg caatttggac actttgccca
     8401 catgttatca ggaacagatg gcgagttagc aggtttgctc tctctattag gattgatccg
     8461 aatatatttc tttttcttaa acagagccat ctatgcgcct cctatctccg tttctggtgt
     8521 ccaattcggt aaaaattctt cttgtaaaaa tgctgtgctg taatcacctg ctatgactgc
     8581 agggtgacta atcaaatcca tctgaaattc tgcgttcgtg atgattcctt ctgtgataaa
     8641 ttcacttaag gctcgctgca tcttcattaa tgcttcaaaa cgcgtatttc catggacgat
     8701 tatttttgca atcattgaat cataatatgg cgggatcgta tatcccgggt aaactgcact
     8761 gtctacccgc agtcccatcc cacctgctgg aagcagtaag ttttggatct tccctggaga
     8821 aggagcaaaa tggaaagcag gattttctgc attaatccgg cattcgattg cgtgtccttg
     8881 gaatactact tcttcttgac acacattcaa aggctcaccg gctgctatct ccacctgttt
     8941 ttttactaaa tcgattcctg tgaccatttc tgttactgga tgttccactt ggattcgagt
     9001 attcatttcc atgaaataaa aatcgccttc ttcatccatc agaaactcga tcgttccggc
     9061 attttcataa ttgactgctt tagctgctcg aacagcagcg tctcctagtg cttctctttt
     9121 tgtttgtgag atcactacag aaggagattc ttccagtact ttttgattat tacgttgcaa
     9181 agaacaatct cgttctccta aatgaatgac attgccaaaa tgatcaccta aaatctgaac
     9241 ttctatatgg cgagcaggat aaatgatttt ttccaaatac atatcatcat ttccaaatgc
     9301 cgctgctgct tcttgctgag ctgaagaaaa atgctgtggg agctcttctt ttgattgaac
     9361 tttccgaatg cctttcccgc caccacctgc agctgctttc agcatcactg gataaccaat
     9421 tctgtctgct atttccaatg cttcttctac tgtagataat acaccagtac ttcctggaat
     9481 gactgggaca tttgcttctt gcatcaattg acgcgcattg atcttatttc ccattgcatc
     9541 gatcgttgca cttttaggac caataaatgt gatattgcac tcttcacaca tggaggcaaa
     9601 tcggctattt tcagataaaa aaccaaatcc tggatgaatt gcttcggctt ttgtcacgat
     9661 tgcagcgctc agtacttcct gaacattcag atatgaatca acggcttttg ccgggccaat
     9721 gcagattgct tcatctgcta actgtgtatg aagtgcttcg gcatctgctt ctgaatatat
     9781 cgctacggtt tggatcccta gttcgcggca tgcacgaatg atacgtacag cgatttcacc
     9841 gcggttagct atcaatactt tagaaaacat atttcaccta tccaatcata aaggttaatt
     9901 ccgcttcggc tactttttta ccgtctactt tagcgattcc tttgcctatt ccggctgaag
     9961 ctttcacttt caatatctca acttcaaggt ataaggtatc tcccggtgta actttcttac
    10021 ggaattttgc tttgtccaat ccgccgaaat aagccgtctt tcccttgaat tgttcaagag
    10081 aaagtaatgc aactgctcct gcttgagcca tcgcttcaac aattaatacc cctggcataa
    10141 caggttcttc tggaaaatgt ccttgaaaaa agggctcatt gatcgttaca tttttcttag
    10201 caacgatccg ttctccttca accatttctt ccactcgatc aatcaaaagc attggataac
    10261 gatgtggaat gatttctttt atttcttgta tgttcattga tttccctctt tcctgctttg
    10321 cttatttttg ctgaatccta aagagtggct gtccatattc gacaactgac tcgttttcta
    10381 caaggatttc tgtgatcact ccgtcggcct cactagttat ctcattcatc agtttcatcg
    10441 cttcaataat gcagataact tctcctttgg caacttgatc acctactttt ttgaaagctg
    10501 gtttgtcggg cgccggttgc agataaacga ttcccacgat tggcgaaaca acctcgattc
    10561 catcaaatac tgccgattct ttttctttca ttgtttctgg agcagttgtt gcttgttcag
    10621 gtatgacatt gactggctcc gaaactggca acgtttcgtt tgtttgaacc atattcatgc
    10681 ctcgacttgt tttattttta ttcatataca attcaaattg accttcacgc aaatcgaatt
    10741 ccgttagcga ggattgatca aattgactga ctaattctct gatttcattg atatccaaaa
    10801 ttattcctcc cagcgtttca tgcacagaac cgcattatgt ccgccaaacc ctaatgaatt
    10861 actcattgca taagtaaaac ttgcaggttt gctttcgttt ttgacaactg tcaagtcaat
    10921 ctcaggatct tgtttttcta cattgatcgt aggtgggata aattgatgtt ctaatgccag
    10981 caaagttgcc aaggcttcga tcccgccggc tgctcccaat aaatgtccag tcatcgattt
    11041 cgtgctgctg acgtaagtat ctctgaaatt ttcacctaat gcgatttgga ttgcttttgc
    11101 ttctgcctgg tcattggctg gtgtacttgt tccgtgagca ttgatatatc cgacttgttc
    11161 cggctgtaca ccagcttctt ccatcgcaag cttcattgct ttagacgcac cagaaccatc
    11221 tggagtagga gctgtcatat ggtaagcgtc acagtttgca ccgtatccaa tgatttctcc
    11281 atagattttg gcaccgcgag cttgtgcatg ttctaaagat tcaagaacta gaacacctgc
    11341 tccttcaccc atcacaaaac cattccgatt ttcgtcaaac ggaatcgaac cacgattagg
    11401 atcagttgat tctgagagtg ctgttaaagc tgcaaatcca gcaatcccca tctcacaaat
    11461 cgttgcttct gttcctccag caagaatgac atcagaatag ccatgtttga tgttacggaa
    11521 tgcttcccca attgaattcg ttccgctagc acaagctgtt acgatagctg tacaaatacc
    11581 ttttgcacct acgcgcaaag caatatttcc agctgccatg ttagcaatcg ccattggtac
    11641 gaacattgga gaaactcgtt ttgcaccttt ttcatgtaga cgaataactt ggttttcgat
    11701 tgttggaaga ccaccgatcc cagatccgat gatgacgcca aaccggtctt gattcatttt
    11761 ttctttatct aaaccgctca tctccattgc ttctaaagca gcgtagattc catagatcga
    11821 aaacatatcc attcgtttgc tatctttttt aataaaatat ttatcgtaag ggaaatcttt
    11881 gacttcaccc gctaaggtaa tccctgtttc tgaagcatca aatttagtga tcggtgcgat
    11941 gccattcgtc ccctctttta agctattcaa aaagctgtct gcgtcatttc caataggtga
    12001 agtgactcca tagccagtaa ttaccactcg attcatttgt ttgctctcct tttcttatcc
    12061 gtgcatgacc aatccgccat cgacattgat gacttgtccg gtgatatatg gattttgtgc
    12121 taagaaaact gctgtttgtg caatatcttc tacttgtccg aaacgctgca tcggaatttg
    12181 cttttccgct tgttccttga ctttgtctgc taaaacttcc gtcatatcag ttgtgatgaa
    12241 tcctggcgca atggcattac atgtaatgcc tctagttgca gcttctcgtg ccacagattt
    12301 tgttaatcct actacgccag ctttactagc tgcataattt gcctgaccaa tatttccaat
    12361 caacccagat acactagaga gattaatgat ggccccttct cgctgcttca tcattttttt
    12421 caatacgtgc tgcgtcatat tgaaggttcc aatcaaattg atatcaagac attttttgaa
    12481 atcttctgca tccattcgca tcacaagttt gtcatttgtg attccggcat tgtttactaa
    12541 cacatggatc gatccaagct tttcttctgc ttctttgatc atttgtcccg ctttctcata
    12601 atcagagatg tcaccggaaa ctcctacgca ttttacaccg aatgcttcga tttcttctat
    12661 cttttctttt ggaatctctc cgcgaccatt caaaatgata tttgctccag cttttgcaaa
    12721 agctaaggcc atcgctttgc cgattcctct tgtactacct gtgataaata cattttttcc
    12781 ttttacatcc ataacgttcc tccttatagt attgctattg tttcatcaaa tgtctgctta
    12841 ttctcaacac gcagaactgg tattgtttta tcgatttttt tcataaatcc gcttaatact
    12901 tttccggggc cgacttcgat gacttgttcg atgccgattg tttttaactt atggatactc
    12961 tcatagaaac gaacaggttg catgacttgc tgttcaagta gtcccgcgat tgtttctttc
    13021 ttcattattt ccgttgttgt gttgcttatg ataggaaaag aaggttcaga gaattgaatc
    13081 tgacttaggt ccttcgccag tttttttgct gccggttcta aaatagctgt atgaaaaggc
    13141 ccgctgacat ttagagggat cattcttttg aacccttttt cttttaatag taaaacagct
    13201 tcgtctactg ctttttcttc gccaccaatc acgatctgct gaggtgtatt ataatttgca
    13261 ggagatacga tgccgtactt gctagcttca tgacagcttt cctcgatcgt ttcaatcggc
    13321 gcattcatca ctgcaaccat tttcccgctt ccagctgggg cagcttctgt catataagcg
    13381 cctctttttg ctactaacgc aactgcttca gtgaaggata gcgcattact agctactaat
    13441 gccgaatatt ctcctaaact cagtcctaaa gctgcatctg ggatgattcc atgctccttc
    13501 aataagcgat aatacgcgat gctgactgtt agaatagctg gctgtgtata ttgcgtctga
    13561 tccaaacgtt cattttctgt aaagcaaagt tcagccatat cgtagccgag aatctcactt
    13621 gcttcatcaa atgtctgctt tacaacggct tcttcatata aatcttttcc cataccttga
    13681 tattgtgcac cttggccact aaataagaat gctgttttca tgtttttctc caatatatcc
    13741 agaatatatg tttgattctt cctattcaaa aaggccgaga cattcctatt tcaggaaatg
    13801 tcccagcccc caacctaatt ttaatgatta tgcagtttgt ttttctacgt aagtcactaa
    13861 atcttgaacc gttttgattc cgtcttctgt ttcgattttc acgtcaaatt catcttcaat
    13921 ttcattgatg atttggaata aatccaagct gtcagcttct aagtcttctt ggatgtcagt
    13981 agtcaatttg atttcttcag tttcttttcc taattcttct gcgatgattt cttgaatttt
    14041 ttcgaatacc ataataatat attcctccga tattgtttgc tttttatttt tataatttaa
    14101 cagtctacaa ctccatgaga atacttcccc atgttaaacc tccgccatag cctgtaaata
    14161 ttactttctg ctgttttccc aaggtgatct ttccactaga aactgcttca tccaataaga
    14221 tagggatgct tgcagcagat gtattcccat atttatccat attggtcagg aacttttctt
    14281 gcggcatctt tacttttcgg gcaattttct caataattct gaggtttgcc tgatgcaaaa
    14341 gcagataatc aactggtttg tctttcatga cttcttggat attgtttgca acgtctcgta
    14401 cggcaaaatc aaaaatatct ctgccagtca tttgcaaata atacgaacct tctgaatctt
    14461 ctgaatagaa cggattttta ttttgctggt agccagcggt caatgagcta cttctggtac
    14521 cgtcagctgc taatttttct ttcagcaatt ttttctgtgg attcgcttct aagagaatcc
    14581 cacctgctcc atcgccaaac aacacagcag tcgtacgatc agaccaatcg attagcttag
    14641 atagtgtttc accaccaatc actagtccct tagttgagcc gcagcggatc attttttcag
    14701 ccatgcttaa cgcataaaca aatcctgagc acgcagcact gatatcaaaa gcgatagcat
    14761 gagttgctcc caatttccct tgtacttgtg ctgctacaga tggcgtcgtg taatcgggcg
    14821 acattgttgc tactaaaata aaatccaaat ctttagggtt tagatttttc tgttttatca
    14881 atttttccgc cactaaaatg cataaatcag aagtctcctg atttgttaca catcttcgtt
    14941 cacgaatacc cgtccttgac gaaatccatt catcagaggt atccatcatc ttggaaagat
    15001 cattattagt aataatcttt tcaggaagga cgctggctgt tgcagtaatc attccaaatt
    15061 gttccattaa atatcctcac ttatagtctt gcaagaagtc gtgcaagttt ttcagtgcat
    15121 ccagcaaggc atgctgttcc tccgtagcca ttccgtctaa gatgttcttg accatttcac
    15181 gatgaaaatg ctgatggacc cgaaatagta actttccttt tttcgttagt cccaacttta
    15241 caacgcggcg gtcatcttca ctgcggatac gttcgacata gccttttttt actaacttgt
    15301 taatagcagt ggtcaaggtc ccaacagtaa ttgatagttc tttggctact tctgaagttg
    15361 tttttttctt gtacatcccg attgcctcga tcgtgtgcat ctctgtgatt gacagatctt
    15421 tgaattgaga ttttttcagc tcagactctt cgattgtcaa aatatcattg aagacactaa
    15481 caagataatc atttactgtt tttaaatctg gttccatgtt ccaccgtcca ctaattagtt
    15541 tgattatcaa atcttttgat gttcaaacta tatcttaact ttt
//
LOCUS       DAXWOO010000057        15288 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.057, whole genome shotgun sequence.
ACCESSION   DAXWOO010000057 DAXWOO010000000
VERSION     DAXWOO010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.057"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            82..1170
                     /locus_tag="ACGE70_001400"
     CDS             82..1170
                     /locus_tag="ACGE70_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFF8693044.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            1298..2674
                     /locus_tag="ACGE70_001401"
     CDS             1298..2674
                     /locus_tag="ACGE70_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFF8693045.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            3075..4655
                     /locus_tag="ACGE70_001402"
     CDS             3075..4655
                     /locus_tag="ACGE70_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFF8693046.1"
                     /translation="MNNPELKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            4853..5077
                     /locus_tag="ACGE70_001403"
     CDS             4853..5077
                     /locus_tag="ACGE70_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HFF8693047.1"
                     /translation="METEWQIRERKKQKKEEMLRLCRLLDLKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(5282..5602)
                     /locus_tag="ACGE70_001404"
     CDS             complement(5282..5602)
                     /locus_tag="ACGE70_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFF8693048.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(5750..7984)
                     /locus_tag="ACGE70_001405"
     CDS             complement(5750..7984)
                     /locus_tag="ACGE70_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFF8693049.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            8463..8729
                     /locus_tag="ACGE70_001406"
     CDS             8463..8729
                     /locus_tag="ACGE70_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFF8693050.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            8729..10456
                     /gene="ptsP"
                     /locus_tag="ACGE70_001407"
     CDS             8729..10456
                     /gene="ptsP"
                     /locus_tag="ACGE70_001407"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFF8693051.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            10620..11666
                     /locus_tag="ACGE70_001408"
     CDS             10620..11666
                     /locus_tag="ACGE70_001408"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFF8693052.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYSIRVPIQAISNGIDLEKYRPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            11683..12906
                     /locus_tag="ACGE70_001409"
     CDS             11683..12906
                     /locus_tag="ACGE70_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFF8693053.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            12995..13882
                     /locus_tag="ACGE70_001410"
     CDS             12995..13882
                     /locus_tag="ACGE70_001410"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFF8693054.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            13968..14198
                     /locus_tag="ACGE70_001411"
     CDS             13968..14198
                     /locus_tag="ACGE70_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFF8693055.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(14255..15220)
                     /locus_tag="ACGE70_001412"
     CDS             complement(14255..15220)
                     /locus_tag="ACGE70_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFF8693056.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRAQLPDLQ
                     EMTFEQKKKELQGLFQGQNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKHFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMIASADVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQSEIELLNKKEAEKQLN"
BASE COUNT         4968 a         2621 c         3116 g         4583 t
ORIGIN      
        1 aatttttaac gaatataata tatactagta agaaagaaag gaggaaatct attgtttgaa
       61 aaacttagac agtctaaact aatgttttgg tcgcttgaat tattgatcat agctgcattg
      121 atcatgattt catcagaaat aaactttatt ttcgaaccga tcgggacatt tttttccacg
      181 ttatttgcac ctgtactgat tgcaggcttt ctttactatt tattgaatcc tattgtcaag
      241 ctgttgatga aagcaaaaat caaaagaata tgggctgtcg caattgtgct gctcctttta
      301 gttgcagcaa ttatttggat tttacttagt gtcatcccaa gtctagtaca gcaaatttcc
      361 tcattggcat ctaatatgcc tgatttcatc aaacaggtag aaagctggtt aaaagaggta
      421 gccgaattgc ctttgttcaa ggaagtagat atcaataaat attttgagca attagacatt
      481 tcttacggga caatcattca gcaattcctt agtggagtat ccaatagttt aggttcgatc
      541 gttggaacga tcgcttcggc aaccgtagta atcattacag caccattcat cttgttctat
      601 atgttgaaag acggagaaaa actagtgcca aacatccaac agtttttccc agaaaaaaga
      661 agaaaccaaa ttgtggagtt attggggcaa ctgaataaaa cattggctaa ttacattagc
      721 ggacaagcaa tcgaatgttt attcgttggt accttcacat ttttaggata tatggtaatt
      781 ggtgtggatt atgctttctt atttggggtc attgcgggat tgacaaacct aattccatat
      841 ttaggacctt atctaggact agcacctgca tttctagtaa cagtcttcaa tgatccggta
      901 aaagcattac tttgttgtgt ggttgtgcta atcgtccaac agttggatgg gaatatcatt
      961 tatcctaatg taattgggaa atcactgaaa atccatccat taacgatcat tatcgttctt
     1021 ctagtagctg gaaatattgc cggattgctt ggtatcttct taggtgttcc tttttacgca
     1081 atctgcaaga cgatcatttc ctatataatc aaaatcgtca aagaagacaa gcaaaatgaa
     1141 aacaagaaga aaattgctac gacctcgtag gtagttttag tacacttgtt tttattacaa
     1201 agaaaattgc tttgtgatac cattttaacg tggctatagt gccacgtttt tttctatcgt
     1261 aaactaaaaa ttaattttat aaggagagaa tgcgattatg aatgctgacc ctgagagtca
     1321 gtcgctgtta gcgcagattt tattattgat cgttttgaca ttgatcaatg cttttttagc
     1381 agcttccgaa attgctgttg tttcgatcaa taagaatcga atcgagcaaa aagctgatga
     1441 aggagatgtg aaatctcaaa agcttttaaa aatattgcag aatccaaata atttcctatc
     1501 gacaatccaa gtaggaatca ctttggtcaa cattttgtct ggtgcttcac tagctaatac
     1561 tttatcggaa cggttagctc cggtattagg tggcggcaca gcggcaaaaa atattgcaaa
     1621 tatcattgtg ctggctattt tgacctatgt atcgattgtt tttggagaat tgtaccctaa
     1681 acgtattgct ttaaataaat cagaagaagt cgcaaagttc acttcagggc tgatacgctt
     1741 gattggtgta gtagctaagc ccttcgtctg gctgctttct gcttccacaa gtttattggc
     1801 gcgtatcacg ccgatgactt ttgatgatga agattcaaaa atgaccagag atgaaatgcg
     1861 ttatatgctt gaaaacgaag gcgtattgaa taatgaagaa ttagaaatgc tccaaggggt
     1921 tttttctctt gatacgaaag ttgcccgaga agtcatggtc ccacgaacgg atgcatttat
     1981 gattgatatc aacgacactg tggaagaaaa tgtgaatgaa gttttatccg aaaactattc
     2041 aagaatccct gtttataacg aagacaaaga taaagtagtc ggaatccttc atacaaagaa
     2101 tttactaaaa gctgcccata aattcggttt tgataattta gatatcaaga aaatcatgca
     2161 ggagccttta ttcgttcctg aaacggtctt tattgatgat ctgttatatg aaatgaaaaa
     2221 gacgcagaat cagatggcta ttttattaga tgaatatggc ggcgtcgttg ggttagcaac
     2281 actagaagac cttttagaag aaatcgtcgg agaaatcgat gatgaatcag atgaagttga
     2341 gaatctttat gaaaaaatcg atgaacacga atatatcatc caaggacgta tgctgattga
     2401 tgagttcaat gaagcttttg acagtgatct gcatatgagt gatgtagata cgatggctgg
     2461 ttatttgatt acagcattag gcatgattcc ggatgaaggc gaaaagttat cgtttgatgt
     2521 cgataatatt acgctggttt ctgaagaaat ggaaggttct cgaatcctta aaatccgtgt
     2581 catttttcat gatccggaag aaacagaagc ggaacctgaa gaagaacgaa gatacttcag
     2641 aaaagaattc gaagatgacg agccaagaag ataattggaa acttggttat gaaagacgtg
     2701 ttgcttgaat aaacaagcaa cacgtctttt cttttagtca gctgacaaaa aaatatttga
     2761 ctcttatggt ttggtggctt tttcattcga tgaatccatg ctatactgat tcagttgtag
     2821 tagttgacaa aggttgtgaa aaagctatcc tgcattaagt aataagagca gccaagcaaa
     2881 aacagctttt cgtatttttg gcttgggtgg gcttattgct gataggctgt ctctagaacg
     2941 ccgtttactc ggaaaaagag actgctacac ggcttttgtc acagccctat atgaaattgt
     3001 ctttctttaa gagatcggac aatctgtgcc tagagaaaat ataaataatt gaatggaaga
     3061 aatggagtta ggttatgaat aatccagaat taaagcaaca agtggacaat cgcagaactt
     3121 ttgcgatcat ttcccatccg gatgctggga aaacaacgat tacagaacaa ttactattgt
     3181 ttggtggggc tatccgtcaa gccgggaccg taaaaggcaa aaaaacaggt aattttgcca
     3241 aatcagactg gatggaaatc gagaaacaac gtgggatttc tgttactagt tctgttatgc
     3301 agtttgatta tcaagggaaa cgagtaaata tcttggatac accaggacat gaagattttt
     3361 ctgaagatac ttatcgtact ttgatggccg tggatagtgc ggtcatggta atcgatagtg
     3421 caaaagggat cgaagcacag acaaagaaat tattccaagt tgtaaaaaaa cgtggcatcc
     3481 cgatatttac ttttatcaac aaactagatc gtgatggtcg tgaaccattg gaattgttag
     3541 aagaattaga agagctatta gatatcgaat cttatccgat gaattggcca atcggtatgg
     3601 gtaaagggct agaaggtctt tatgatatct accataatcg tgtagagttt tatcgtccgg
     3661 aaaattatca ggatgaacga ttagtagaat tggatgaaga tggactgctt cccgaaaatc
     3721 atcctctaac tgaaaattcc ttatatgaac aagtcttaga agaagtagaa ctaatcaaag
     3781 aggctggaga tgcatttaat caagaaaaaa ttgctcgtgg cgatcagaca ccagtatttt
     3841 ttggttctgc attgactaac ttcggtgtac agactttttt agagactttc gttgaattgg
     3901 caccagctcc ttatggacac aaaacacaag atgatcagat ggtaagtcct tatgaggaag
     3961 aattttcagg tttcgtattc aagatccaag caaatatgaa tccagcccat cgtgaccgaa
     4021 tcgcttttgt ccgaatctgt tctggaacat tcgaacgagg aatggatgtc tggttggaac
     4081 gtacaaataa aaaactgaaa ttaagcaatg tcacacaatt tatggcagat tcgagagaaa
     4141 acgtcgaaaa agcagtagca ggcgatatca tcggtgtata tgatacaggc aattatcaga
     4201 ttggggatac attgttcgaa ggaaaactaa aagttgctta tgaagaactc ccttcattta
     4261 ctccagagct gtttatgaaa gtgactgcaa aaaatgtcat gaaacaaaaa tccttccaca
     4321 aaggaatcta tcaattagtt caagaaggag cgattcagct ttataaaaca tatctaacgg
     4381 aagaatatat cattggagct gttggtcagc tacaattcga agtattccaa tatcgtatgt
     4441 taaatgagta taacgccgaa gtaatcatgt ctccaatggg acacaaaatc gctcgctgga
     4501 tcgatccaga agatttggat gagaagatga gttcaagtcg caatatcttg gctcgtgacc
     4561 gtttcgacca accattgttc ttattcgaga accaatttgc agaacgctgg tttgcggata
     4621 aatatcctaa tgtaaaatta aaaagtttga tgtaaagaag tcttgacaca agctgccctg
     4681 catcaagtaa taagaatagc caaacaaaaa cagtctctca tattttgttt agagccgtga
     4741 cggattttcc gtgacggctc tttttactat attaaaatca tagaaatgtc taatcaaaaa
     4801 aatataaaaa ttattttgcg tacatatgat taaagaaagg agagtgtgac acatggaaac
     4861 agaatggcaa atacgtgaaa gaaaaaaaca gaaaaaagaa gagatgttac gattatgtcg
     4921 attattagat ttaaaagact gggacaaaaa cgagcaaatt gcacaaaaag tacgatcaat
     4981 catccataaa gaggaaaaag ttaaaaataa agacacgcgt agaactgaaa atgaaaagag
     5041 aaaagtacaa aagaaaagag aaaaatcaaa aagctagaaa gaaaaagagg ctgagacaga
     5101 agcgtttaac tccaagaaat aagctggaat tcacaaaaat ttgaaaagca attttcgtga
     5161 attttagctt atttccgaag gagttgcttc tgcttccgcc gtttatacgt gtttagagcg
     5221 tgagacaaaa gtgttcttta cttttgtccc acgctcttta attattatgt tcaaaatttg
     5281 tttacacggc taatgtagcg cctaattcat gttttggaat cgagatttca acaaggcctg
     5341 ccaattcgat tactttgatt tcatcctcgt ggtaaacggc gtctttgatt ttatctaaga
     5401 aataattttt agcatcttcg atttctttcg gctggatttt acgtttgtca ttgatcagca
     5461 gaatttcatt atgttcaggg gcttcagtgt agacaatagt ggtatttccg gctgtgtaga
     5521 ccttgactag atgggcatca gtgttttcaa gctggtttaa tacaagacgg gaatggctgt
     5581 ttgttacatt taccaatttc atggcgctca cctcttttat gtatttttaa ttgcttgttt
     5641 ctcttattat aatttctttt ttataaaatg aacagtattt tgacttttct gaaaaaagaa
     5701 tgagacggca tttctcaaag aaataccgtc tcacgctata aaactggatt tattcagccg
     5761 tttcttcggc tgattcttta gccaaacgtt ctggtttaga agtaacgatg atttttccat
     5821 ctttatccag ttttgctttc aactcatgga tagaagggtg atcaagataa aattcagcga
     5881 taccatcttc gatttgttct tggatcgtgc gtcgaagagg acgagcaccc atagatggat
     5941 catagcctaa gtcaactaat ttttctttga cattcgtagg aacttcgatg tgaagttgct
     6001 gtgctgccag catttggttg acatcgtcta gcattagtgt gacgattgtc atcaagtttt
     6061 cttttgataa tgcactgaat tcgatgatcc cgtcaaaacg gttcagaaat tctggtgtaa
     6121 agtagttgtt taattggttc aatacagaac gagtaacacc ttcgcgtgcc gcaccaaaac
     6181 caacgtttgc ttctacttta ccagttccgg cattacttgt cataatgatg atcgtatctt
     6241 taaagctgac cgttcttcct tgtgcatctg tcaatcgacc atcatctaag atttgcaaga
     6301 acatatgcaa aacatctgga tgagcttttt ctacttcatc taacaagaca agactgtaag
     6361 gatttctccg tactttttct gttaattgac cagcttcttc gtatccgaca taacctggag
     6421 gagaaccaat caatttagag acactatgtt tttccatata ttcagacata tcgaaacgaa
     6481 tcattgaatc ttgtgaaccg aatagttcgt aggcaagttg tttcgcaagc tctgttttac
     6541 ctacaccagt tggtccaaca aataagaagg agccgattgg acgattctgt ttgtttaatc
     6601 caacacggtt tcggcgaatc gctttcgcta ctcgatctac agcgttatct tgtccgatga
     6661 catgagcttt caagtcatct gctaagtttt tcagttgtgt ctgttctttt tcttttaatt
     6721 cacccactgg aatacctgtc cgttgttcaa cgattttttc catatctttt tcggtaatca
     6781 caggtgtttc ctcttcagtg agctgacgtt ctttcatttt ttgaagttta ttgatctgat
     6841 cgcgataata agctgctttt tcgaaatctt cttctcgaga agctaaaact ttttgttctt
     6901 ctgcttcttg cagttttttc tcgattgttt taggatcaac gatttggatc gtcaagtttt
     6961 tcttagaacc tgtttcatct agcaaatcga tggctttatc cggcaagaag cgatcttgga
     7021 cgtaacgatt ggataaagtt gcggctgctt cgatggcctc atcagtatat tttacatgat
     7081 gatagtcttc ataacgtttt tgcaaacctt tcaaaatact gatcgtttct tcgacagttg
     7141 gttcatctac ccgtacaggt tgcatacgac gttctaaagc agcgtctttt tcgatgatgc
     7201 ggtactcatt taaggtagta gcaccaacca tttgcaattc tcctcgagct aaagctggtt
     7261 ttaaaatatt accagcatcc atatttccat caccagcaga accagcaccg acgatttcgt
     7321 gaacttcatc tatgaatagg atcacatttt ctgcttgacg gatctcttca atcaattttt
     7381 gcatacgttc ttcaaattgt cctcgtatac cagtaccttg gactaaagat acgacatcta
     7441 aacgaatgac ttctttgtcc attaattttt gcggtacatc accatctacg attttttgtg
     7501 ctaaaccttc aacaactgct gttttaccaa cacctggttc tccaatcaag acaggattgt
     7561 tcttggttcg gcgatttaaa atttcaatga ctcgtttgat ttcgtcatcg cgtccgatca
     7621 caggatcgat atcaccttgt cgtgcttgtt ctgtgatatt gattccgtat tctcccaata
     7681 aaccttctgt attgccttga ggtggctgac caccattgaa gttgttgttt ccgccaaatt
     7741 gtgttggtgg tgtttgctca taatttcctt gctgttgcat ttggcgagac atggaacgga
     7801 aaagatcgtc caaactgcca aatccaaatg gatcattttg ggccatatta gtcagacctc
     7861 cattttgttg atttttgatt ttttgataac agctctgaca gtaatcgagt tgtgtccgct
     7921 gaccattgac agtggcatac aagtgaatcg ttgcctcatt cttgccgcag ttttgacaaa
     7981 gcatgaatca ttcacgtcct ttcacaaaaa tgggtacatc catattttac atctcgtttt
     8041 tcatgaggta aaagaataac ccctagtttt ttaagagatt tatgaggtag aaaaggtgaa
     8101 atctctattg accattcctg acctttgact cttattatac taaaaataaa aagggaagca
     8161 agtaattcgt ttcatatgaa aacaaagagg gtcaaaggaa agaatgataa acgtcaggag
     8221 aagagaaagc gacaagaaaa agaaaataaa cacgtttgtt ataaaatgtg aagaaaagga
     8281 tgaaaaagaa tccggaataa tttgaatact tttatctgaa attgaatgaa aactgttgta
     8341 tctaacgaaa aaaaatgtta atctttacag atgaaagggt ggcatttgtt aggattctct
     8401 gaattactaa aaaagaaaac cctcacataa aaacaacact cacttaaagg agaccgatta
     8461 atatggaaaa gaaagaattt cacgtagtag cagaaactgg gatccacgct cgtccagcta
     8521 cactattagt acaaacagca agcaaattta actctgatgt aaacttagag tataaaggta
     8581 aatcagtaaa cttgaaatca atcatgggcg ttatgtcttt aggtgtaggt caaggttctg
     8641 atgttactat tactgcagaa ggtgctgacg aagctgatgc aatggcagca atcgttgaaa
     8701 caatgaagaa agaaggctta tctgaataat ggttgaaatg ctaaaaggga ttgccgctag
     8761 tgacggtgta gccgttgcaa aagcttacct gctagttcaa ccggatttaa cattcagtaa
     8821 ggcaacagta gaagatactg cggctgaaga agctcgtttg gatgctgcat tagctaaatc
     8881 tactgaagaa ttgcagcaaa ttcgtgaaaa agcagcgcaa agcttaggtg aagcagaagc
     8941 gcaagtattt gatgcccatt tgatggttct ttcagatcca gaaatggtag gtcaaatcaa
     9001 gcaaaatatt aaagacaaca gcgtaaatgc tgaatcagcg ctaaaagaag taacggatat
     9061 gtatatcggt atgttcgaag caatggaaga taatgcttat atgcaagagc gtgctgcaga
     9121 tattcgtgat gttgctaaac gtattttagc gcacttatta ggcgttactt taccaaatcc
     9181 atcaatgatc aacgaagaag tggtcgttgt tgcacatgac ttgactccaa gtgatacagc
     9241 acaattagat cgcaattttg taaaagcgtt tgtaacagac attggtggac gtacttcaca
     9301 ttctgctatc atggcacgtt ctttagaaat tccagcaatc gttggtacaa aagaaattac
     9361 tgctaaagtc aaagaaggcg atattttggc tgtcaacggg atcgaaggcg atgtaatcat
     9421 cgatcctaca gatgaacaaa aagctgaatt tgaaaaagca ggtgcagatt acgcagcaca
     9481 aaaagcagaa tgggaaaaat taaaaaatgc agaaactgtc acagctgacg gcaagcattt
     9541 tgaattagct gcaaacatcg gaacaccaaa agatctagtt ggtgtccata ataatggtgg
     9601 agaagcagtt ggtctgtacc gtactgaatt cctgtatatg gattctccag acttcccaac
     9661 agaagatgac caatatgaag cttacaaagc tgtattagaa ggtatggaag gaaaacctgt
     9721 agttgttcgt acaatggaca tcggtggaga taaagaacta ccatatctgc aattacctca
     9781 tgaaatgaat ccattcttag gatatcgtgc attgcgtatc agtttatctg aacaaggcga
     9841 cgaaatgttc cgtacacaaa tgcgtgcatt gcttcgtgca tctgttcatg gtaacttacg
     9901 tatcatgttc cctatggtag ctacactcaa agaattccgc gcagcaaaag cgatctttga
     9961 agaagaaaaa caaaaattga tcagtgaagg caaggaagta tctgatacga tccaagtcgg
    10021 catcatgatc gagattccag cagcagcagt cttagcagac aagtttgcaa aagaagtaga
    10081 tttcttctct gtaggtacga acgatttgat tcaatacaca atggcagcag accgtatgaa
    10141 cgaacgcgtt tcttacttgt accaaccata taacccatca attttacgtt tgatcaaaaa
    10201 tgtaattgat gcagcacacg ctgaaggaaa atgggcaggt atgtgtggag aaatggctgg
    10261 agatcaaaca gctgttccat tgctagtagg tatgggtcta gatgagttct caatgagtgc
    10321 gacatctatt cttaaaacgc gtagtttaat gaaacgcttg gacacagcga aaatggctga
    10381 acttgctgat cgtgcattga aagaatgcga tacgatggaa gaagtcgttg aacttgttca
    10441 tgaatatgta aaataaacaa cgtatcaaaa gctggtgtgg cttgtaaaag agctacgcca
    10501 gtttttttgt tcttttttaa gaaaacgttt ttgtcagtaa gaatagataa aaatagggct
    10561 taagtttgag tgacagtttg taggctatgt tatactatat aagtgaaggg ggacgtaagg
    10621 tgaaggtatt attatatttt gaaagtgaaa agatgcttgc aaaatctgga atcgggcgtg
    10681 cgcttgatca tcaaaaacgt gctctgacgg aagtcggtat ctcttataca ctagatgaaa
    10741 aagaagatta tgacatcctg cacatcaata catacggaat caatagtcat aatatggtca
    10801 ataaagccag aagagaagga aaaaaagttg tctatcatgc gcattctaca gaggaagatt
    10861 tccgaaattc ctttatcgga tcgaaccagc tttctcctat cgtaaaaaaa tatctggtag
    10921 gattgtacca aaaagcggac tatctgatca cgccaacacc ttattcaaaa cagttgttgg
    10981 aaagctatag tatccgtgtg ccgattcaag ctatttccaa tgggatcgac ttagagaaat
    11041 atcgccctga tccaatgaaa gaacaaaagt tcagagaata tttcaaactg tctccggatc
    11101 aaaaagtaat catttgtgtc ggtttgtttt tcgaacgtaa aggaatcatt gatttcgtag
    11161 aaattgctaa gaagatgccc gaatacacat ttatttggtt tggtcatgtt cccatgtact
    11221 cgattcctcg gaacatccgg aaaattgtaa aagaagatca tcctgaaaat gtcctgtttc
    11281 ctggttacat tcgtggagag atcatagaag gtgcgtattc aggtgcagat ctattctttt
    11341 tcccttctta tgaagagaca gaagggatcg ttgttttaga agctttagca agcaaacaaa
    11401 acgtacttgt tcgagatatt ccagtgtata atggctggtt agaagatagc aagaactgct
    11461 atatggggaa gacaaacgaa gaatttatcc gactgatcca acagattacc aatcaagaac
    11521 taccgagtac gacacaagcc ggttatcaaa cagcaaaaga gcgaagtatc aataaaatcg
    11581 gtgaagaact taaaaatgta tacgaaagtg tactgaatga aaaagtctct agcaaattaa
    11641 aaaaagtaaa tcaggtcaag gactagtttg gaggcgaaaa acatgaaaat cggctttttt
    11701 accgatacct actttccgca agtcagcgga gttgctacat cgatcaaaac attaaaacaa
    11761 gaattagaaa gacatgggca tgaagtctat atttttacaa cgaccgatcc taatgcggat
    11821 aagttggaaa aagatgtgat tcgaatgcca agtgtaccat ttatttcttt caaggatcga
    11881 agagttgttg ttagaggcat gtggtatgct tatttggttg ccaaagaact agaactagat
    11941 cttattcata cccatacaga attcggagca gggatacttg ggaagatggt agctaagaag
    12001 ttgaaaatac ctctgatcca tacttaccat acgatgtatg aagactacct tcattatatc
    12061 ggtaaaggaa aagtgatcag gcaatcccat gtaaaatact tgtctagatt atttgcaaat
    12121 catacgacag gtgtagtttg tccaagtgaa cgtgttattg atacattacg tagctatggt
    12181 gtaatagcac cgcttcgcgt gattcctact ggtatcgaca ttgaaaaatt caaacgttct
    12241 gatatcactc agcaggatat cagcaaatta agggagtcac taggtcttca ggaaaaccat
    12301 ttgatgattc tttctttaag caggatttct tatgaaaaaa atatccaagc cttgattaat
    12361 gggtttcctc aggtcatctc cgcttatcct aacgccagat tagttatcgt aggaaaagga
    12421 ccttatgtag aagagttaga ggaattgatc tcagaactgc agttagaaga atttgtgcag
    12481 ttcacaggag aagtggacaa caatgatgta gcactttatt ataaagcggc agattatttt
    12541 gtcagtgctt ctacttcaga aacacaaggg cttacttata cagaagcgat ggcagccgga
    12601 acaccttgtg tcgtggaagg aaatgcttat ttgaaccgat tgttcgatca tccttcatta
    12661 gggaaaactt ttgaaaccga cgaagacttt gcaccagcac ttattgatta tatttccagt
    12721 gaagttccta aagatccaaa agttttagaa gctaaacttt atgatatttc atctacacat
    12781 ttcggcgaag ctatgatcga attttatcaa gacatggttg tttatcatga agaagagatg
    12841 cgaagaaaag aagaagaaga ctcgattgaa cgaatcaaaa taaaattaaa ttcatttaaa
    12901 agataaaatt caaacatttt ttatgaaaaa atccttgttt ctagcgtata ctagcataga
    12961 agcaaggatt tttgattttt aggaggtcga aaaaatgaaa cttggtttta tcggaacagg
    13021 cgtcatggga agtgctgtcg cacgccatct actagaagca ggacacgaag tagctgtcta
    13081 taatcggaca aaagcaaaag ctgatccttt agtaacagaa ggagctatct gggcagatac
    13141 accaaaagcg gtagcagaac aaagtaatat tctctttaca atggtaggtt accccaaaga
    13201 tgtagaagaa atctactatg gacagtcggg gattttttct gcagatatct caggacatat
    13261 tttagttgac ttgactacaa gtacaccaag cctggcagaa aaaattgcaa aaacagcaaa
    13321 agaaaaaggc gcagatgcgt tagatgcacc ggtatccggt ggtgacttag gagcaaagaa
    13381 tggtacactg acgatcatgg tcggtggaga agaagccgtt tatgatcaag tactgccttt
    13441 attcaaagaa tttggtacga catttacatt gcatggttct gctgggaaag gtcagcatac
    13501 aaaaatggct aatcagatca tgattgcagg tacgatgaca ggaatgacgg aaatgcttgt
    13561 ctatgcacaa aaaaacggac ttgatttaaa gaaagtgatt gagacgcttt caggtggaag
    13621 tgcagctaac tggtctttaa gcaattattc tcctcgtatt ttaaaagaag attacacacc
    13681 tggttttttt gtcaaacatt ttatcaaaga tttaaagatt gcattagaag aagcagaaaa
    13741 aatggacctt gtattgccag caactaccca agcattgaaa ctatatgaag agttggcaga
    13801 taaaggtttt gagaatgacg gaacccaagc tttgatcaaa ctttggtggc ctgaaggaaa
    13861 aataccagaa aaaaaatcat aaaaaacgag tttttttaga gaaaatcaat gcaagtaggc
    13921 tatgcttttg atacaatata acagaaataa gaaaaggagg acctccgatg acacattcac
    13981 aattagtcgc aatcataaaa cgactggagg ctatggtcga gtcagcagat aatgaactgc
    14041 aagttcgccg ttttgaaaaa gaaggcgtag agaagtgtac agtaacttat gataaagcga
    14101 ccgatacatt tgaattaact gaaacagata ctcatcagca atacgaattt gataatatcg
    14161 atatcgtagc gatggaaatc tatgacttaa tccaataaat tttagaataa ttgacatttg
    14221 aagttgaaga tgcctaaaag aagccgagaa gcaactaatt tagttgcttt tcagcttctt
    14281 ttttgtttaa cagttcaatt tcagactgtt ctttttctaa ttttttgcgg taactgatca
    14341 agatttgtac aggatcgatt tcttcttttg acaattgacg tttcaaagat aaatgagctt
    14401 ctttcttttc ataatagaag tcaggagaaa cgtcagcaga ggcaatcatt gtttctgcaa
    14461 gttgaccata acaaacgagg tagtgctttt tctgactctc atctgctgct aatggcatgc
    14521 tgaaggtgag cgatggtgtt cttaaaaaat gcttttgttc ctggatccgt ctaataaaat
    14581 aagtaactaa ttcgatatct ttttgaagca taccggcttt ttcgaaattc aactgataga
    14641 tgaaatgttg ttgtcttttt ttcaagtaag ttagtagctt tttattttgg ccttgaaaca
    14701 gcccttggag ttcctttttt ttctgttcaa aagtcatttc ctgtaggtca ggaagctggg
    14761 ctcgcaaagc taacaaacta atttcagaca accaaggcat ttgataagtt tcttccatta
    14821 tacgcaaaat agacggcatt tttttataga gacgaaaagg cccgtatgtt cgagccgtag
    14881 gagtatctgt taatacaaat cccttattgg taatatgtac ataattataa ttactgaaat
    14941 aattcatttg tcggttataa aaaggacggt aatgttggat caattgacac tcgagcaata
    15001 aagcatctaa ttccgtgtct actacgacga aatcaaaatc tgttatatga tgaatcatac
    15061 gtaatacttt ttttgaatgc tgtttattct gatgaaaata gctgcttact ctatttttga
    15121 gccgttttgc tttaccaaca tagatgacct taccatcacg gtctttcata agatagacac
    15181 ccggcagcat ggggagagtt ttcatttttt cttttaacat aggatctcct tttacgcttt
    15241 gaataaaaag actataacaa attttgaaat aatggacaag ttttaagg
//
LOCUS       DAXWOO010000058        15100 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.058, whole genome shotgun sequence.
ACCESSION   DAXWOO010000058 DAXWOO010000000
VERSION     DAXWOO010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15100
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.058"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(21..251)
                     /locus_tag="ACGE70_001413"
     CDS             complement(21..251)
                     /locus_tag="ACGE70_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693057.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            complement(687..1253)
                     /locus_tag="ACGE70_001414"
     CDS             complement(687..1253)
                     /locus_tag="ACGE70_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFF8693058.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1253..2209)
                     /locus_tag="ACGE70_001415"
     CDS             complement(1253..2209)
                     /locus_tag="ACGE70_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFF8693059.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2209..2865)
                     /locus_tag="ACGE70_001416"
     CDS             complement(2209..2865)
                     /locus_tag="ACGE70_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFF8693060.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3235..5253
                     /locus_tag="ACGE70_001417"
     CDS             3235..5253
                     /locus_tag="ACGE70_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFF8693061.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    5323..5529
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5680..8094
                     /gene="leuS"
                     /locus_tag="ACGE70_001418"
     CDS             5680..8094
                     /gene="leuS"
                     /locus_tag="ACGE70_001418"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFF8693062.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8442..9995
                     /locus_tag="ACGE70_001419"
     CDS             8442..9995
                     /locus_tag="ACGE70_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFF8693063.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10376..12160)
                     /locus_tag="ACGE70_001420"
     CDS             complement(10376..12160)
                     /locus_tag="ACGE70_001420"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8693064.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12175..13896)
                     /locus_tag="ACGE70_001421"
     CDS             complement(12175..13896)
                     /locus_tag="ACGE70_001421"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8693065.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13996..14757
                     /locus_tag="ACGE70_001422"
     CDS             13996..14757
                     /locus_tag="ACGE70_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFF8693066.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4906 a         2771 c         2978 g         4445 t
ORIGIN      
        1 ctcattaaaa agcataacca ttactccgat ctgtttttaa aattagtttc tacctctaat
       61 atatatcgag tttttatttt gccttcagag aatactgttt ctttttttgc agttacaggt
      121 tgtttatttt cggaaaaagc taatatagct aagattgaaa catccatctg gaatttatct
      181 tttttactgc tttgatcata aaagtctgca tattcatcac tgatattttt tctaataaat
      241 tcttccatca taaaaatcac ctcaaaacga ttatacgaac ttacgttctt gttgtaaagc
      301 gatatttgag gggcaaaaaa ggggcataat ttgactgata ttaagttagc tattgatata
      361 tgaatgacat acaaaaatat aaaatctttt aaaaatgctt atatatcaat gtttttgcca
      421 ttcttttata tatctgttat atgtcaaaaa aatgtgttca atttcaattt gtatttgaat
      481 aaagaggttg tgaaaaagct gtcctgcatc aagtaataag aacagccaat caaaaacagc
      541 ttctcatgtt tttgatttgg ttggacttat taccgcagat ataagctttt gagcaccgtg
      601 aataaagagg ttatgaaatc aacgctctaa cctgagtatt agagaaaaaa acgcaaaaca
      661 tatcatttat gttttgcgtt ttttatttat ctttttttct caatacaata taaaataggc
      721 ggctggttct tttggttgat aaaccgataa ttcagcacat tatatgtttc ctgtggcagt
      781 ttggaacaaa aagaatcaac catatccagt tctttttctc caccttcatg accatagtag
      841 acgacaagga tcaatcgtcc gcgaggcaca agtcgtttca atatttcttc catagctgtg
      901 cgtgtagtct ctggcatagt gatgattgct ttatcacttt ttgggagata ccctagattg
      961 aaaatccctg ctttgagatt ttgttgttca gctatcactt ctcctaacgt ttcgtggcct
     1021 tgtagaaaca atgagacacg ttcttctaac tgacgctgtt ctagcctttc acgagtagca
     1081 tcgattgctt gttgctggat atcaaaacta tagacatgac cagtttttcc gatgtgttct
     1141 gctagaaaaa ccgtatcgtg gccattgccc atcgttgcat ctacgacgtg atcccctggt
     1201 tgcaagattt cttgcagtaa tgtatggctg aaatgcaaag cagtttgcaa catcagacgc
     1261 tcaccttttt ccgtacatca tattttcctt ggaagctatc tctgcgaaga agttcttcat
     1321 caattgcatt caagacttcc cattttttca agctccacat tggtccaatc aaggaatccc
     1381 acggcgcatc ccctgtcaaa cgatggatga tgatctcttt tggtatcatt tccagctggt
     1441 cgcaaatgac attgacatag tcaggacggc tcattaattg aaggcgacct tcatgatagt
     1501 cccgcaacat tctcgttttg cgcatcagat gcatcaaatg gagcttgatt ccttggatat
     1561 ctgaatctaa aatggtgcgt ctgacatttt ctttcatcat ttctaaactt tcgcctggta
     1621 atccgttgat caaatgcgta caaacacgga tattatgttt gcgtaattta gccacgccat
     1681 ctaaataagt ttgataatca tgtgctcggt tgatcagttt tgaagtttct tcaaaagttg
     1741 tttgcaatcc aagttctacc cataaataca tccgctcgtt cagttcagca aggtaatcaa
     1801 ctacttcatc tggcagacaa tccggtcgtg taccgactga taatcctacg acacctggta
     1861 aattgactac ttgctcaaat cgttctttga ttatttcaac tggcgcatgg gtgtttgtaa
     1921 aattttggaa ataaacgatg tattgttgga catgaggcca tttttgatgc atcatgtcga
     1981 tttctttctg gaattggatt ggaagagggt cttcaggtgc aacgatcatg tctcccgaac
     2041 cagatacact gcaaaaagta caaccgccat gggcaacagt cccatctcga ttcggacagt
     2101 caaatcctcc atcgatcggc accttgaaaa ttttcccgcc aaactcattt cgtaaagcgt
     2161 aattccaaga atgatatctt ttatttcctt ctgaataagg aaaagtcatt aaaagtcctt
     2221 ctttctgtaa gagttatcta tttgttttct tttttctcta tacactggat aagtcaggca
     2281 cagccaagaa agactgagta agtagccgcc gatgatatca gtaggaaaat gaacgcccag
     2341 ataaatgcgg cttatcccga cacttactat aataaacatc atcagcaatt gaagcaatgt
     2401 gcgtaaggtt cttttttgaa caaaagctgg aagcagaaat agtaaagaac cgtaaagaat
     2461 catactgcct gctgcatgtc cactagggaa actgtaactt gtttcaacta ctaagtgcgc
     2521 caatgaagga cgctctcgca tgaaaaatag tttgatcacc gggttgatga ttccagcaat
     2581 tacaataaat ccagaaacga accaaaatgt ttctgccctt tttttcttat accacatcca
     2641 taaagccgca caagcaaata aaatgatgac ggtaagagga ttaccgaatt tagtcataaa
     2701 gagttggtag ctgttcagat caggataaga agctctgaca atgcctgtaa accactgatc
     2761 gaaaccagaa agccacgaca gatggaactt tacgatccag cccaaaatga aaaacaggac
     2821 aagaaagaag ctaccagcca cctgaaatga tgttttatat ttcatacaat tcctcctttg
     2881 aagcaatgac ttattatagc atttttggtc cagcgaaaaa aacggtaaag agataaaacc
     2941 aatgtatgat tcctctttga ataaccaata ttcttataaa aaaaataact gtaatcggaa
     3001 agacattttt tggtaatgca aatgaaatgt tagggaaata aaatcagtgt ttttcgaaat
     3061 ccttttaaac aagaacgtag acgtaggaag aacttgaaaa tgaacagaag ttgttcatta
     3121 cggtaaatta caatatgatt acaaaaaaca acaaagctct taaataactt ttaataaata
     3181 ttaagagaag ttataattat taataataga taaattgaag ttggaggata gaagatggaa
     3241 aacattgcaa gaaaagaacg gagacgttta gaacagacca aacgttttcg aaaagcaaaa
     3301 cgaggggcag caatcgtggg aactgcgatg gtaggctgtt ctgtcgctgc cccattagta
     3361 cagccggttc ctgttcaagc ggatgaaaca cctgttcagt tcggcgcaag aatcaataca
     3421 gcggcattta tcacagaaat tgctacttat gctcaaccaa tcgctcaagc caacgatcta
     3481 tatgcatcag tgatgatcgc acaagcagtt gtagaaagcg gttggggcag tagtgcgtta
     3541 tctcaagcac cttattataa tctttttggg ataaaaggca gctatcaagg acaaaccgtc
     3601 tacatggata cgcttgaatt tttaaataat caatgggtaa ataaaaagga gcctttccga
     3661 caatatcctt cttttgctga atctttcaat gacaatgcct atgtacttag aaatacttca
     3721 tttggcaacg gttattacta tgccggtact tggaagagca acacgaaatc ctatacagat
     3781 gcgacagctt gtttgacagg gcgttatgcg actgatcctg gttacgcagg caaattaaat
     3841 aatatcatcg caacttacgg attgacccaa tatgatacac cagctagcgg aaacgcaggc
     3901 ggtggagcga ctacaggtgg caataatact ggaagtgcag gtagtactgg cggttcaagc
     3961 aattctggta atacaaactc aggttcagca acttcgacca cttatactgt ccaatctggt
     4021 gattctgttt ggggaatctc aaataaattc ggtatcacaa tggatcaatt gatccaatgg
     4081 aacaacatcc aaaacaattt catttatcca ggacaaaaac tgacaatcaa aggtagccag
     4141 gaaaatggct cttcgacgaa taattccggt aacaatacca actcttcagg aaatgctggt
     4201 acatcaaacg gaggacaaac aactggtgcg aaatacactg ttcaatctgg tgattctgtt
     4261 tggaagatct caaatgatca cggaattacg atggctcaat tgattgaatg gaacaatatc
     4321 aagaacaatt tcgtttatcc aggacaacag ctaatcgtat caaatggcgg ttcttctaat
     4381 gctggagcaa atactggctc aaatactggg aatacaagtg gctctagttc gtccagctca
     4441 agttcttcta atacagcttc aggaacgaaa tatacagtta aagccggaga ctctgtttgg
     4501 agcgtctcaa ataaatacgg aattacgatg gctcagctaa tccaatggaa caatatccaa
     4561 aataacttta tctacccagg acaacaactg actgtttcaa acagtggatc aacaaacagc
     4621 acgtcaggta atacaggttc gtcaaccaat acaaataaca gcagttcagt taatactggc
     4681 agcagttcgt ctcaaggcac ttataccgtg aaagcaggtg aatctgtttg gagcgtggca
     4741 aataaaaacg gcatcacgat ggatcagtta atcgaatgga ataatatcaa aaacaacttc
     4801 atttatccag gacagcaatt gatcgttaaa ggtggaactt cggcaaatac aaatacaggt
     4861 tcaactactt ctgcagcgaa accaaataca ccgaatacgt ctgctacgac aagtacatca
     4921 tccactggta atacgatgta taccgtgaaa gcgggcgagt cagtttggag cgtagcgaac
     4981 aaacatcata tcacgatgga tcagttaatc gaatggaata atatcaaaaa taatttcatc
     5041 tatccaggac agcaagtgat tgtgaagaaa ggttcatctc aatcaacaaa ccaacaatca
     5101 gttactggtt cgaaaaccta tactgtgaaa tctggagaat ctgtttggag tgttgctgac
     5161 agtcatggaa ttacaatggc tcaactaatc gaatggaaca atattaagaa caactttatt
     5221 tatccaggtc aaacgttgat tgtagctaaa taaaatagct gttcgaagaa ataacgattg
     5281 aattccacaa aaatatttgt tactataagg ctacttaaat atgacaacac ttgaaaagga
     5341 agtagtaaaa agactgaagt ttcagagagc gcatggctgg tgagaatgtg cacggatgct
     5401 tttgaactcg cctttgagtt gcccttgctg aatacccgag taagcaagcg gcggtgacgg
     5461 ccgttacaac gttcgaggcc ttttcactaa ggtaaaatta ggtggtaccg cgtagattac
     5521 gtcctataaa aagagcaatc tttttatagg atttttttat ttcttttgtg aagtttgaga
     5581 aaacaaagca aaaggaaata aaaataaaca gattctgccg gttttttcgt tatatttata
     5641 atagaaacaa gtgttttcaa tagaacagga ggacagcaaa tgagttacaa tcataaagaa
     5701 attgagaaaa aatggcaaaa atactgggca aaaacgaata ctttcaacac ccatgatgat
     5761 cctgaaaagc caaaatttta cgcactggat atgtttccct atccatccgg tcaagggcta
     5821 catgtagggc atcctgaagg ttatacggca acggatatcc tttcacgtgt gaaaagaagt
     5881 caaggataca acgtgctgca tccaatgggg tgggatgcat tcggtctgcc tgctgaacaa
     5941 tatgcgctag atacaggaaa cgatccagct gagtttaccc aaaaaaatat cgaaacattc
     6001 cgacgccaaa tcaattcttt aggatttagt tatgactgga atcgcgaagt gaatacaact
     6061 gatccggagt attataaatg gacacaatgg atctttacga agctttatga aaaaggtcta
     6121 gcttatgaag cagaagtagc ggtaaactgg gtacctgaat taggaacagt tatttcaaat
     6181 gaagaagtga tcgatgggaa aagtgaacgt ggtggctatg atgtagtcag aaaaccaatg
     6241 cgccaatgga tgctgaaaat cactgcttat gctgatcgct tattggatga tctagaatta
     6301 gttgattggc cagaaagcat taaagaaatg caaagaaatt ggatcggtcg ttcagtagga
     6361 gcaaatattg aatttaaagt ggctggtacg gataaatcct ataccgtatt tacgacacga
     6421 ccagatactc tattcggtgc aacttactcg gttcttgcac ctgaactaga tcttgttaga
     6481 gaaattacga cccctgaaca aaaagctgct gtagaagctt atatcgaaga gacagctaaa
     6541 aaatcagatc taaagagaac ggatcttgca aaagaaaaaa caggagtatt tactggtgct
     6601 tatgcaatca atccagttaa tggcaaggaa atcccaatct ggatcgccga ttatgtatta
     6661 tcttcatacg gcacaggagc catcatggct gttccagcac atgatgaacg tgactttgaa
     6721 ttcgctcaaa ctttcggact agaaattctt ccagttatcg aaggtggaga tgttgaaaaa
     6781 gctgcttaca cagaagatgg tacgcacatc aactctgaat ttctagacgg catgaacaaa
     6841 caagaagcaa tcgataaaat gaatgaatgg ctagaagaaa atggcgtagg gaaaaaagaa
     6901 gtcagctatc gcttacgtga ctggctattc tcccgtcaac gttactgggg tgaaccaatc
     6961 ccaatcatcc attgggagga cggaacagtc acagctgtcc cagaagaaga gctaccatta
     7021 cggttgccta agacaaaaaa catcaagcca agcggtactg gagaatctcc attagcaaat
     7081 atcgaggagt gggtcaatgt cgttgatcct gtaacaggta aaaaaggccg tcgcgaaacg
     7141 aatacaatgc cacaatgggc aggaagctca tggtattacc tgcgttatat cgaccctcat
     7201 aacaaaaaag aactagcaga ttatgaaaag ttgaagcgtt ggctaccagt tgacatctac
     7261 attggtggag cagaacatgc ggtacttcat ttactatatg cacgattctg gcataaattc
     7321 ttatacgata tcggtgttgt tcctacaaaa gaaccattcc aaaaactata caatcaagga
     7381 atgatcttag gcgaaaacaa cgaaaaaatg tcaaaatctc gtggtaatgt agtcaatcct
     7441 gatgatgtgg tcgaaactta cggtgcagat acactgcgca tgtacgaaat gttcatgggg
     7501 ccattagatg cttcgattgc ttggagcgaa aatggcttag aaggaagccg taaattcttg
     7561 gatcgtgtat ggcgtttgat cgttgatgag aacaataaaa tgcgtgaccg tatcactaca
     7621 ttgaatgatg gcaagcttga taaagtgtat catcaaacag tgaaaaaagt gacagaagac
     7681 tatgaaaatc ttcatttcaa tacagcaatc tcccaattga tggtctttat caatgaagcc
     7741 tataaagtgg acgctctgcc ttatgaatat atcgaaggat ttgttcaact actcgcaccg
     7801 attgctcctc acatgggtga agaactatgg gcaatccttg gaaatgatgg tggtatttct
     7861 tacgcaccat ggccaacata cgatgaagca gcacttgttg aagacgaagt agaagttgtt
     7921 ttccaagtaa acggtaaagt tcgtgcgaaa agcaatgttc cccgagactt aggaaaagat
     7981 gagctagaaa aagcagcatt agccaatgaa atagtgcaag aatatattga aggcaaaaca
     8041 gtcagaaaag ttattgcggt accaaacaag ttagtcaata tcgttgctaa ttaattggct
     8101 ctttgtcaat aaggactgat gagttgtgca aaatcaaatc tgggccagaa tgaacttcat
     8161 tctgtctcag attttttgtt atttcaaatt gattaattga ttgatcaaaa aggttttgat
     8221 tttcatgttc tcaaacacgt tcaatgtctt tttcttctga atggtcttgt aagagatctc
     8281 tgtaatcatc aaatttttgt ctattaatct tagtattttt tgatgtataa actacctgaa
     8341 attgagagta cataccaaat tcataaattt tttattagtg ggactagtga ttgtatggat
     8401 tttgacgcat aatgaatgaa gtatgagaaa aggagagaca aatggatcaa gaactattta
     8461 ttatcgaagt attatcagct aaacaaaaaa ttatcgagaa agcgaaaaaa agagcgaaaa
     8521 gaataatgtt caagctttta agcttagtat ttttctctgg tttgcttttt ttcggagctg
     8581 tatcgttcct atatcgccat atgagtgcag tagtccgttc agctgaaggg atgcaaaaat
     8641 taaataattt gtatcacttt tcgtggggag ttggcggaat aacaattgtt gtgcttattg
     8701 tttttcttct acttatttgg ctccgttacc atcaattgtc gcgtcatttc gatcaatggc
     8761 ttagtcaaag agcacaacag tttttacata ataaaccaac ttctcaagat gatgactatc
     8821 tttattttga gaagacttcg acgagtcaag cttttcggat acaaaaaagt gattgccagt
     8881 tattgctgag atcacttgat gatttaccta tctacttagg cgaaagcaaa tcaaaattcg
     8941 gaatgaacac attccatatt tttttactag aaaacgaacc cagatgggaa aaaagccagt
     9001 cttcgcctgg tggatacctg acaactaaga tgagtacatt agtcactggt tttttcctca
     9061 ttcttttagc tggaacaatc tattatggga gatacactca gccaaaaatg tcttctcata
     9121 taaatgctgc ttctcacatg acaatgaact caactaacga tacgcatata caaaagacaa
     9181 atgaagggtt gttagctgat cctaagcaaa aggtaaattt gacaacacaa aagataaatg
     9241 atctcaaact tgatacagag acgcactctc tctatatgac aacagatagt ggtgctaatt
     9301 gggtatttgt gccgattgat tcgcaatggt tgcgttttgg cgaatacacc ctgacaacgg
     9361 ggctgattcc tgaaggttat tggatggaca acacatatga tatttccgcc gatttctcat
     9421 ggtttatcta ttcttctggg gatgatgtac gtctgctgac ttcaagagat aatggtaaaa
     9481 catggcaaac gtctattatc acccaatcag ttcagccgat acgttatcga aaagcggtat
     9541 tttatggcaa cgatggtcta tttatctgta catcagccag tgggatgtct gcagaaactt
     9601 tgtacattta cactactatg gatggcggaa tcacttggat aaaaagtgga agtacaatga
     9661 tcaatcagcc agtacaaaat gtcagcttcg ttacaacttc tttaggtttt gtttcgacac
     9721 gtgaaagttt gttttataca aacaacggag gacgctcatt caaagaatca gttctgtcga
     9781 ttccggaaaa ctatcagaca ggggggatag atctttttca atcaccaaat gaagttactc
     9841 aaataagcgc gaatacgtta gagacaaaat tttatttgtt gaaaaaagga ccaatcgatg
     9901 cagggaaaat gtttgcctgc ctcttccgtt caactgataa cggggaaaca tggcaattcg
     9961 ttcagcaact atcgcaagta gaacaaggag attaaagaag agagattgtg agcctgacgt
    10021 tcagcaccga gtcacaagaa acaaattcaa aaaacagctt ctcatatttg ttgaatagtg
    10081 taactcgtga ttcggagttg acaagagcaa caagaagcga ttttgaagta tagcttctcg
    10141 tattttacaa gatcgtgtct tattgccgat aggttgtctc ttgaacaccg tttattcgta
    10201 aaaagggatt gtgacaagtc ttttgtcact acttaccgaa cgaaactgaa ggagctatcc
    10261 aaaaagtatc attagtatta ttctttgtgt ttctagtggc accactagta catttaaaaa
    10321 agctatcgat catggactaa tcatacatga tcgatagctt tttagataat gtttgttacg
    10381 ctatctgaaa ttgactatta taaagactgg cataaagcgt tggtgattgg agtagttcag
    10441 aatgtgttcc tttttcgacg atatcgccat tcttcattac aaggatcatg tctgcatttt
    10501 caatcgttga caaacggtgt gcaatcacaa aacttgtcct atcttttgtc acagcttcca
    10561 ttgcttgctg aatcaatgct tctgttcgtg tatctacgct agaagttgct tcatctaaaa
    10621 taacgacagg aggattcgct agaatgactc gagcaattgt caataattgt tgctgtcctt
    10681 gagataacgt gccgttttcg ctagaaatga tcgtatcata tccgtctggc agtgttcgaa
    10741 taaagtgatc acattgtgcg atttcagccg cttcgataat ttgttctctt gtggcatcca
    10801 ttttcccgta ggcgatatta tcagctaccg ttccttcaaa cagccaggta ttttgtaaaa
    10861 ccatcccaaa agaacgacgt agttctccac gtgaaagctt ggtaatatca agaccgtcga
    10921 attcgatctt tcctttattc agttcataaa aacgcatcag cagattgact aaagtcgttt
    10981 ttcctgcacc agttggacca acgatcgcta ccgtttgttt tggctttact gaaaaatcga
    11041 cttgattcat caatatttta tctgatgtgt aaccaaactg tacatttttg aaagctattt
    11101 ttcctttcgg ttgttctaaa agtcttgcat ctggaaggtc tggaatttca tctgtttcat
    11161 ctaatatctc aaatatccta tcgattgctg ccattgcgga ttggatcgag ttgatcacat
    11221 aagaagcggt agaaattggt tcagaaatct gattgatgta ctgaagataa gcttgtaaga
    11281 agccgatcgt cacaccacct gatagaacaa gcatagcacc gaataccgca cttatgatga
    11341 aggcgagttg attaatgaaa cgaatggccg gatagatagc acagttcata aattgagcac
    11401 gtttaaaagc gttgtattgc tttttattta cttcgtgtat agcagcttct gcatgagctt
    11461 gctgattgaa tgttttgacc actagattac cagctaagat ttcttccatt cgattattta
    11521 aaacacccag ttcatactga ttctgttcag aaatctgttt gtttttgttc gcaatcttag
    11581 ttgtaacaat tgaactgcca acgatcagca ataagacgag caaagtaagc ttgctttcga
    11641 tataaaacag catcagacca gcagcagcta ttgttacgac agaagtaaaa aattgattga
    11701 tacctgtcaa taacacttga gaaagttgat tgagaccggt agttgtccga ctaatgatgt
    11761 cacctacttg atgattgtca aaaaacgaca tcggtaatgt tttgaatttt tttgtgattt
    11821 cttttcgcac cctcaatgtg atcttttcgc ttaacgctgc cattgtccgt tcctgaataa
    11881 atgaagtgat gctgcttaag actgaacagc cgatcagcaa taagacaggg aacaacaatg
    11941 cttgtttgat atcactgaaa gaaagctgtc caatacctgt ttttcttatt tctgataaga
    12001 gatcatcgat cccgattccc atgatcaagg gcatggctac gactaatagg ttcccgaata
    12061 aactgcatag aaacaatccg taaaaatatt tatgttcagg tttgatcatt ttccaaaaac
    12121 gtatcaaagc acgcgtgttc aacgtttttt tcttttccat cgtatccctt ctttctatat
    12181 tccttgtgat tttgcaaact cctgataagt ttggcaatta gacagtagtt cttcatgagt
    12241 accttgtcca acgatttttc cttcatctaa aacaatgatg ttatctgctt gttggattgt
    12301 actgaggcgt tgagcaacaa tcacgagagt tttctccgac atgtgtttat gaagggctgc
    12361 tcttaatgct gcatctgttt tatagtctaa agcggagaaa ctatcatcga atacatagat
    12421 ctctgctggt ttgatcaacg ctcgagcaat acatatccgc tgtttctggc cccctgaata
    12481 attagaaccg ccttgagcaa caaagctgtc caatccgtca ggaagggtgt ccactagttc
    12541 ttctaattga gcgatcttga tcgctttaga caactcttct gttgaggcat attcatttcc
    12601 catctgcaag ttgcttaaaa ttgtcccgct gaataagaaa gccttctgag ggacataact
    12661 gatgtgggag cggatctgtt cttgagtcat ttgagtaaga ggaataccac caagggttac
    12721 ttttcctatc gtagggtcac taagtctcaa gagaagtttc gccaaactac tttttccaga
    12781 tccggtcccg ccaacgatcg ctgttgtttt tcctttaggt attgtgaaat caatatcttc
    12841 tagaactgga tcagagattt cagtatattt aaatgtgaca tgttcaaaag cagctgcatt
    12901 tgtctgatca aaggttggtt cagctactgt tttgatcgtc tcttcctcaa tttcgatctc
    12961 tgcttgcagt atttcttcta tccgtcgtaa tgaggcaaac gatttaggga taatgaccaa
    13021 taccatagct gcgatcatca agtaagcaag tgtcagcata gagtattcga ctacagcagt
    13081 cacgacaccg atttgtaatg ctccttctgc tacaagattt ccgccaaacc acaaaataga
    13141 agcataaacg attcccatga gcaaaaaagc aattggagtg acccaagcaa atattcggtt
    13201 cactttgatc atttgattag catagtcagc aaagctctta ttcgttcgct tttcttcata
    13261 tgattgatta tcaaaggcac ggatcatatt aatcccagta aagaattctc tcaatgtgat
    13321 ggtgatccga tccatttttg gctggatact caaagataaa ggtgcccctc gtttcatcag
    13381 tcgataaaca atccatacat aaaaaacaac agagatcaat gtaacccatg ttagtaatag
    13441 agaataacga aacatcatga gaatggtgaa aacgcaaata accggtgctg gtaagatcat
    13501 ttggcaaaat aaaacaatca tttgctggac attatcgaca tcattcgtca ttcttgtcaa
    13561 aagagatcca gaactaaaac gactgatatc cttcattgac agctgttgga atttacggaa
    13621 atatttctcc cgcgtttgga atccaaacct agcggaagcc aatgctgaaa agtaactaga
    13681 ggcaactgca gcgattgttc ctagaaacgc tacaagcagc atttcaactc caattttttt
    13741 tataactgag gcttcgccac ttgctatacc tgtgtcgatc agctttgcta ctaattttgg
    13801 tacacctaat gtaccggcga tttctaaaca gatcatcaca aaggtaagga gagcgagtcc
    13861 tttgtttttc tgtaagaatg tttttaagat agacatatta ttcctccttt tttcctgata
    13921 cttttcgatt ctataccttc aagtaacttg aaggtcaata gaaaaaagaa taaactgaaa
    13981 aagaggtgag aaaaaatgaa aaaaatgtat acgattggcc aagtatcgga actatttgat
    14041 ttgccaaaat caacgatccg ctattgggat gaacaaggat taatccgttc aagtagacaa
    14101 gaagagaatg attatcgatt gtttgatatc gatgatattt ttatgcttta tgatatcgtt
    14161 ttttatagaa aattagatat accgatcaaa cagatgaaga atttatatgg aaaaacattg
    14221 acggagttat atgagacact tgatgaaaca gaaagaagaa tccataaaga actggtggtg
    14281 atgaaacaaa aacaaaaaga gataagggag caaaaaaaac agttgaaatt gatgatcgat
    14341 acaaacgagg aagaatttcc tgtagaagag atcccttttg actgtatgat ctcaacggag
    14401 tttgaagata ttgtggaaat caaaaaattt ctacctaact attcaagctt tgggatgatg
    14461 agtatgccag cttccagttc acagacgatg tatggatttt ttatcgaccc tagtgaagta
    14521 catttattta ctcaagacgt gatttgggag aaaaaagata cggctgttta tcgtcgattc
    14581 ttattgaaat ccgagatgaa ccatgcggaa cgtaataata ttttagaaat cagagaaaga
    14641 atgtatgaaa aaggatggaa aacaggagaa gtgatcggcc agtatctatt gacgaatacg
    14701 gatgaaaaca atatcagaac tgaatattat catgcatgga tagaaatgaa aaaataggaa
    14761 atatgtgttt aagataactc gaaaatgatg gttgttttag ttataaagaa aaattttctg
    14821 gttcgattac gggtttaatg tgggggatta ttagtcgtgg atagatagaa ttttagtttt
    14881 ttagaggaaa tatgtcttta aaaatggaat ggttgcaatt cttagcgata tatacgatgt
    14941 aattgtcctc ttaaaaaagt atggaaaaat aattgtattg ggtgttatgt ataattcatg
    15001 gtgaactgag actaatgaga agaaaaattc atattgttat gagtgagatt gtatcagatg
    15061 agaatgataa attaaatagg tgcataaaag cattacactt
//
LOCUS       DAXWOO010000059        14995 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.059, whole genome shotgun sequence.
ACCESSION   DAXWOO010000059 DAXWOO010000000
VERSION     DAXWOO010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.059"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="ACGE70_001423"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="ACGE70_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFF8693067.1"
                     /translation="MAVLARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="ACGE70_001424"
     CDS             complement(322..891)
                     /locus_tag="ACGE70_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFF8693068.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="ACGE70_001425"
     CDS             1077..1979
                     /locus_tag="ACGE70_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFF8693069.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="ACGE70_001426"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="ACGE70_001426"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFF8693070.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="ACGE70_001427"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="ACGE70_001427"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFF8693071.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="ACGE70_001428"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="ACGE70_001428"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFF8693072.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="ACGE70_001429"
     CDS             8547..8732
                     /locus_tag="ACGE70_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFF8693073.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="ACGE70_001430"
     CDS             complement(8794..9990)
                     /locus_tag="ACGE70_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFF8693074.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="ACGE70_001431"
     CDS             complement(10160..11596)
                     /locus_tag="ACGE70_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFF8693075.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="ACGE70_001432"
     CDS             11889..12698
                     /locus_tag="ACGE70_001432"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFF8693076.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
     gene            complement(12884..>14995)
                     /locus_tag="ACGE70_001433"
     CDS             complement(12884..>14995)
                     /locus_tag="ACGE70_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFF8693077.1"
                     /translation="LEKIRKGAQEPKNKTFRRSHVPFRLNFLFFIIFTLFVALIARLG
                     YLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKANQAITYTRGTQVTA
                     KDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKRLSAKEKQLGNSEQY
                     SAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGVTDRELAVVAERASE
                     LPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLAKGYSRNDRVGQSYL
                     EKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSMNAEFQSKVEEILKR
                     NYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEITENALGTITSAFDP
                     GSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRSGQVALDAVKASELS
                     SNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFGLGTTTGIDLPNESP
                     GISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQYASTVANGGKRVQP
                     HLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGFHQVVHGTDPYTTAK
                     PLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEIAISVVLPNLLDEQD
                     HINLTIAKEIMDAYYDMFMAK"
BASE COUNT         4378 a         3179 c         2660 g         4778 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctagtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcat gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gacaattttt gtcacaacct tttttgccga gtaaacggtg ttcaaaagcc
    12781 agtttctcgg tatgaagtca aatttaagca aaaatgtgag aagctatttt cacaaaattt
    12841 ttcttcatac tcgaaactaa gcggcttttt cacgacctct tttttatttt gccatgaaca
    12901 tatcataata cgcgtccatt atttctttag caatcgtcaa gtttatgtgg tcttgttcgt
    12961 ctaataagtt cggcaagaca acactgatgg caatttccgg attgtctgtt ggtccgtatg
    13021 ccacgatatt actattgact gtcgcaatat ctggatgacc ttccgcaacc gtttctgcag
    13081 tccctgtttt tgcggaaagg tccattttcg cactggctaa tggtttggct gttgtgtaag
    13141 gatcggtacc atggacaact tggtggaatc cttcacggag gatcgccata ttttctgcag
    13201 aaatattgac cgtattttct actgttgtgc caatttcttt cttcacttca cccaagtttc
    13261 cattttcgtc attgccatag atcgctttga ctaggtgcgg ctgtactcgt ttcccgccat
    13321 tagcgactgt tgaagcatat tgagctaatt ggattggtgt atatgtgtca aactgtccaa
    13381 atgccaaatc ggtaaagtta cctggagcat accattcttt gccattatcc gcattgaact
    13441 tcttcatata atcaacagaa cgtgagatcc caggagattc attcggcaaa tcgatcccag
    13501 tcgttgtacc aagaccaaac tctttgaaag agtctctcaa ttgctgataa gctttagctt
    13561 cttcatctaa actaggtagt cccattccag gtgtatagtc tagtcctaac atctttaacg
    13621 ccactttgat catatacgta ttagaagaca attccgatgc tttgactgca tcaagagcaa
    13681 cttgtccgct tcgattaaag acagagcttt tttctgaagc accttgcaga cgaatcggct
    13741 catcaatcaa aacttgattt ccgctgatcg cgttacttgc ccagcctgct gtcaaagtac
    13801 cagccttgac tacagaccct ggatcaaagg cgctagtaat cgtgcctaaa gcattctcgg
    13861 tgatttcttt ggaaccttgt tcatgactga aaccagtcat tgctagcact tctcctgtct
    13921 gaggattcat agccactgca taagcaccag gcgaatactg tgctttgccg ttgttgatca
    13981 atgtttgata attccttttc aaaatttctt ctacttttga ttggaattct gcattcatag
    14041 ataacattaa attagaacct ttttccccag agaagatttc tttttgattc gaaacatttc
    14101 cttcattatc aagggaaact tcatattggg ttttggttcc ttgcaggaca tcttcatatt
    14161 gtttttccaa ataactttgt cctacacggt cattccgaga ataacctttt gctaggtatt
    14221 tctctgcttc atctgctgga agtccttgct tttcagtcgt cacactgcca agaatacttt
    14281 tcagtgaact tgctgcatta tattctcgtg tccagtctgt gccagtcgaa actcctggaa
    14341 gttcagaagc gcgttctgcc acaacagcta actctcgatc agtaacacca gagtttttga
    14401 tatagacagt gtttaattca tatgcactat tcatccgttt aaagatggta gcaattttta
    14461 attgctcctc gtttaaatta atctcatcag ctgtgacttt attgaccatc gcactatatt
    14521 gttcgctgtt ccctaattgc ttttcttttg ccgaaagtct ttttgtggct tctttcaaat
    14581 gtttgggatc agccagccaa aaatcttttt tatctctatc tgtcagattt tcatctaccg
    14641 gaacatcgat cagcgagttc agcttcgttg cgatttcaag caaatcttta gccgtaactt
    14701 gtgttcctct cgtatatgtg atggcctgat tcgctttatt cgtaactaaa gcttttcctg
    14761 tggagtcgta gatcattccg cgaggagtac tttcttggac cgtaatcgta gaagtagact
    14821 tcactcgcgc ctccatctct tcgccgttca cgatctgcaa ataacccaat ctagcaatca
    14881 acgcaacaaa taacgtaaat ataatgaaaa aaagaaaatt taagcgaaaa ggaacatggg
    14941 accggcgaaa cgttttgttc ttaggttctt gtgcaccttt tcttattttt tctaa
//
LOCUS       DAXWOO010000060        14684 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.060, whole genome shotgun sequence.
ACCESSION   DAXWOO010000060 DAXWOO010000000
VERSION     DAXWOO010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14684
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.060"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            92..667
                     /locus_tag="ACGE70_001434"
     CDS             92..667
                     /locus_tag="ACGE70_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFF8693078.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            667..1107
                     /locus_tag="ACGE70_001435"
     CDS             667..1107
                     /locus_tag="ACGE70_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFF8693079.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            1123..1824
                     /locus_tag="ACGE70_001436"
     CDS             1123..1824
                     /locus_tag="ACGE70_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFF8693080.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(2312..2737)
                     /locus_tag="ACGE70_001437"
     CDS             complement(2312..2737)
                     /locus_tag="ACGE70_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693081.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            3111..4082
                     /locus_tag="ACGE70_001438"
     CDS             3111..4082
                     /locus_tag="ACGE70_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8693082.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            4114..5511
                     /locus_tag="ACGE70_001439"
     CDS             4114..5511
                     /locus_tag="ACGE70_001439"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFF8693083.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            5670..6293
                     /locus_tag="ACGE70_001440"
     CDS             5670..6293
                     /locus_tag="ACGE70_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693084.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            7042..9135
                     /locus_tag="ACGE70_001441"
     CDS             7042..9135
                     /locus_tag="ACGE70_001441"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFF8693085.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            9295..10161
                     /locus_tag="ACGE70_001442"
     CDS             9295..10161
                     /locus_tag="ACGE70_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFF8693086.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(10183..10620)
                     /gene="ndk"
                     /locus_tag="ACGE70_001443"
     CDS             complement(10183..10620)
                     /gene="ndk"
                     /locus_tag="ACGE70_001443"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFF8693087.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     regulatory      10793..10967
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            11309..13957
                     /gene="mgtA"
                     /locus_tag="ACGE70_001444"
     CDS             11309..13957
                     /gene="mgtA"
                     /locus_tag="ACGE70_001444"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFF8693088.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     gene            14044..>14684
                     /locus_tag="ACGE70_001445"
     CDS             14044..>14684
                     /locus_tag="ACGE70_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFF8693089.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTV"
BASE COUNT         4930 a         2443 c         3077 g         4234 t
ORIGIN      
        1 ataccactaa aaaactacaa aatctagtct tttattttaa aaaaacacgt gtagaatgaa
       61 gatagacttt tgagttaggg agtgtttttt tatgaaactt actgtttttt gcggttcccg
      121 ttttggtaat aaagaaagtt acaaatttat tgcgcaaacg ttaggaaaat acatggcaca
      181 agaagaaatc gaactggttt acggtggttc atactccggg attatgggag tgattagtaa
      241 aacagtactg gaaaataacg gaaaagtcac aggtatctat ccgaatgggc tttttgaaac
      301 tgaattgcct ggaaaagatg ttactacatt tattcctaca gaaacgatcg atgaaagaaa
      361 ggtattatta tttgaaaagg gagatgctgt cctcgttttc cctggaggtc taggtacatt
      421 ggaggaattc tcacaacttc tttcttggat agcaatcggt ctgacgcccg acaagccaat
      481 cggaatattg aatattggcg gttattacag cggcttgcaa caattattag aaacttttgc
      541 aaaagaggat tttatggata aaaaatggtt ggatcaagta atcttttcca acaatccatt
      601 ggaactggtc aatgaattac gagcagtatc tattaaaaat cagttattat tgaaggaggc
      661 aaaataatga gtcaaatata tttagcagga ccattttttt cagaggagca aatcgatcga
      721 gtaagtcgta tcgaaaaagc gttggaggaa aacaaaacag tcacaagttt ctactctcca
      781 cgtcatcacc aagaaagcaa ttatgaacta ttcagcgctg gatgggcgca ggaagtatac
      841 gaaaaagata tggaagaact gacaaatgca gaatttgttg ttgctatcct tgactttgag
      901 catcaaacaa tcgatccagg aacggcttac gaattaggtg tggcaactat gttgaaaaaa
      961 ccgatgatca ttgtacaaga agagacggta cctacgaact tgatgattac acaaagtctc
     1021 catacgtatt taaaatcaga ccaagctgta cgtgaatatg attttgaaac attgcctgtt
     1081 gaaacttacg taggcgagta cttatagaag aggaagggtt gtatgtcagt gattttgatt
     1141 tcaggaacga ttggtgcagg taagagcagt ttgacggata tgcttgcaaa agagattgat
     1201 tctaaaccat tttatgaaaa tgtcgaagat aacgaagtat tgccgctctt ttattcaaat
     1261 cctgagcagt acgctttttt attgcaaatc ttctttttga acaagcgatt tttagcaatg
     1321 aaaaatgctt tagtgaacga tgacaatgtg ctagaccgat ccatctatga agatagcctg
     1381 ttgttccatc taaacgctga tttaggtcgg gtaacggata tcgaagtaca gcagtacgat
     1441 agcttattag atacgatgct aaacgagtta gatgatgttg ctccaaaaaa acggcctgat
     1501 ttgatggtcc atatcaaagt ttctttggac acgatgctag aacgtatcaa aaaacgtggg
     1561 cgtgactatg aacagctaga aagcgatgag acgttatata cctattatga aacgttgaac
     1621 acccgctata atcaatggta tgaagatttt gatgtatgtc ctaaaataca agtggatggg
     1681 gataaattcg attttgtaga aaatccagaa gatgcaaaaa aggtgatccg tcagatccaa
     1741 gaaaaattag ctgaattgga aggaaagcct gctggaaaat attttacgga taaccagcat
     1801 gcaaaaggtc aagaattagg ctaaaaataa aaaaccaaac gacgggaaag aaaaattact
     1861 acgtgatgta gaataagcct ttctatcgtt tggttatttt atattcaaat atttgaaata
     1921 aataaatctt tactctttga tttgtaaact gaaatgatcc actgtttgtt ttactgatga
     1981 agcttgatca aaggaagaag ctgagtttga agttcataaa aatcagccat agtgagtttt
     2041 gcttggattt gtttatcttc ttctactttt tacataattt tgaacaggat aagattcaaa
     2101 tgttttaaat gaatctttga ctagtttgtg acagttgtcg catatgctca agcttccttg
     2161 gtcattaaat tctgtatgga tatttttgtt gttattacat gtacaataat caggactatc
     2221 gatcatccat ttcgtctcct ttacaatcat cttgcttctt tactatagca gaaaagaagg
     2281 ggagaaacaa aagaagatcc aacagacggc actatagact gtagatgttg ccatcctcat
     2341 ccttcaaacg gtaattaccg atttttgtaa atatgactgt tgtttctttt ttattttctt
     2401 ttcctgaaat aatatattcg tcatctttgg tagtgatctt gatatctgtc agcactgttt
     2461 tttcttcaga tgattcagaa gaatcagttc cttgtgtctg atacgtgcgt gtccattcca
     2521 tttttttatc tgtaaatgtc aactgtcccg cggataaatc catatcttta cgtactttta
     2581 gcgtatatac actgttggtg gtatctgctt tttccgcttt taagacagaa gaatcggttg
     2641 tttcgtccga ggattggtca gagccgccaa acaaatcaca gccagtcaat gtcaaaagtc
     2701 ccgcagacaa aataaatagt acgattaatt tcttcaaaat acttccccca tttgatagga
     2761 taaagaaatt ataccattgg aatgtaaaac ttcaaacgga tggaagagaa aaacgcacaa
     2821 aacacccaca aatagagtaa attttagaat aaaattttaa aaaggcaaaa aatccgccac
     2881 tttcagaaag cgttttaaaa aagtgttgca atcttttaaa aaaatgctat attaaaaatg
     2941 aaccggttca aatacttgtt taagtataat taatagtgag aggaagttgc tctactgaag
     3001 actttagtta tatggatatt gatgacaagc aacactatgc aaagaatgcg gtcatcataa
     3061 tcgaacaaca agaaagagaa atcaaatgta actggcagga gtgagaatgt atggcatcga
     3121 tccgtgatgt ggctaagatg gctggtgttt cagtgggaac agtttcccgt tacttgaatg
     3181 gtcagcaact gaaggaaaag aacatgaaaa aaatagcaga agcgatttca gcgttggact
     3241 ataaagaaaa catcattgct aaagggttga agaataatcg aagcttctct attggacttt
     3301 tgataaacgg gatttccagc cgttttgggt cggaagttgt ctcagggatc gaaagagtag
     3361 ctgaagaaaa tgggtatagc ttattattaa gcgggtttgc cgatcagcca gaaaaaattg
     3421 aacaaaaaat tgaatacctg atgaagcatg tgatcgatgg gctgattgtc ttcttatccg
     3481 aagaagagtg gtctggattt gaaatgctca caaaaatgag tattccagtt atcgcattga
     3541 attgtccaga aggaccagca ggtattgata caatattagt gaatgatcga gaaagtgtag
     3601 caaaagtgat cgctcatcat ggtgaacaag gacataaaaa aatcggattt attgctgcca
     3661 ctcaaacaga ctacgtagca agagaacgtt tagcaggagc caaagaagcg gtccaaaatg
     3721 atccatcgat ccaattagag gtctttacag gagactattc tagaaaaagc ggctattacg
     3781 gggcaaaagc attattagaa aaaggggcaa ctgctatttt tgtcagcaat tacaatatgt
     3841 cgattggtgc catcgaatgt ttcaatcaag aaggtgtcag aatcgggaag gatattgcgt
     3901 tcagtcacta tgattattta gataaaaacg atctgagtat ccttccgaaa atcacgatca
     3961 cgccaccaac agataaaatc ggggaacgag cagcagaaca gctgttaaaa cgaatcaaaa
     4021 aagaagagga agaaaatgaa aaagtgattg tgatggaaaa tatcatacat ggaatcaact
     4081 aaaacgaact aaaaaagaaa aagaggtgct ttgatgaaaa aatatcaatt tccagcaaat
     4141 ttttggtggg gttctgcagc aagtggccca caaacagaag gaaggatttc aggcgatgga
     4201 aaaggagaga acatttttga ttattggtac aaaaaagaac cgcaaaagtt ctttgatcaa
     4261 gtaggacctg aaaaaacctc gcaagtttat acgaagtatc aagaggatgt acagttgatg
     4321 aaacagactg ggcataattc atttcgaaca tctatccaat ggagccgttt gatacctgat
     4381 gaatctggaa caatcaacga agaagccgtt cgtttctatc atcgttattt tgatgagttg
     4441 attgaaaacg gtatcgaacc ctttgtgaat ctgtatcatt tcgatatgcc gatgtatctg
     4501 caagaaaaag gcggatggtt gaaccgagaa acggttgaag cctatgaaaa atatgccaaa
     4561 agttgttttg aattgtttgg ttcgaaagta aaaaaatggt ttactcataa tgaaccaatc
     4621 gttccagtcg aagcaggtta tttatatcga tggcattatc cagaagagtc agatatgaaa
     4681 aaagcgatcc aagttggcta tcatgaagcg ttggcttcag cgttagccat caaatcttac
     4741 catgaaatgg agcaaggtgg tgttattggt atcatcttga atctgacacc aagctatcca
     4801 agagacgaat caaaccctga agatgttaaa gcagcgcaaa tagcagatgc gttcttcaat
     4861 cgttcattcc ttgatccagc tgtaaaaggc gagtttccaa gtgaattgat tgagatagta
     4921 aaagaacttg atctcatgcc tgtgatccat gcagaggatt tgacggttat caaagaaaat
     4981 acagtagatc ttttaggtat caattattac cagccacgcc gggtaaaagc aaaagaaaca
     5041 ccaatcgata ctaaaaacgg tccaatgcct gaggattatt tcgacaatta tgaaatgcct
     5101 ggtcgtaaaa tgaatcctta tcgcggatgg gagatctatg aacaaggaat ctatgatatt
     5161 ttgatcaatg tacgagatca ttatggaaat atcgactgct ttatttctga aaacggtatg
     5221 ggtgtagaaa acgaagcacg atttatcaaa gaagatggca tgatccacga tgattatcgg
     5281 atcgaatttg tccaatctca tctgaaatat gtccatcaag ccattcaaga aggtgccaat
     5341 tgtttaggct atcatatgtg gacatgtatg gataactggt cgtggaccaa tgcatataag
     5401 aatcgatatg gctttatttc agtagactta gcacaagatg gaaaaagaac aatcaaaaaa
     5461 tcgggtcatt ggttcaaaga cgtatcagat aaaaacggat ttgaggctta agaaagaata
     5521 gttgagagca aatgcgtgaa attctccatt cttaccattt tgtgaaaaag aagttcaatt
     5581 atttacgatc tttttcaact tttttgtctt tctatttcat tttttgtata taatagaata
     5641 gtcaaaatta aataggagtg gaggaaagca tgaggaaaaa aagaccgttt cttctctttt
     5701 ggttattcgg ttttttctta ctgcttattt tatccgcagc attttcagga aaaatgacag
     5761 cagatcttgc tcagaaagaa acagaaaaaa tcgtagcaag caaagaatca gtagataatt
     5821 tcaaaaaaga attctcaact tattggtggg cggagtataa aataactgcc aattacgaaa
     5881 aaggcgaatt ggacatttat ttgccaaaag gaaaagaaga aaatcaattt attaatatcg
     5941 atgacgtact tagtgcgatt tctctcagac aatatcaatt taaagacaag catttgaccg
     6001 ttttgctttt gactcatgat ggcgacaaag tagcccgttt gaaaaatgga aaagtgatca
     6061 ccgcttattc tgaaaaagta gacaaaaaag tggtcgccga tcgtttagcg gaatggaaaa
     6121 agacaaatga acaagtcaag aaaacaactg tctcttctca aaaaaacgag tcgacaggaa
     6181 caagtccttc agattctggc agtggacaaa cgataacggg aagtgaagaa aatcaagcct
     6241 caattattac gaatgagtca tcacaaggac aatatcaatc ctatggacaa tgaaaaacag
     6301 aggttgtgaa atcagcgttt tgacctgagt attagagaaa caaacggcaa aatagttcct
     6361 catattttgc cgttttttga gctaataccg aaggtcagct gattgaacac cgtgtatcaa
     6421 gaggtcttga gcctgccgtt taactctgag ccacaaggag caatttcaaa aaatagctcc
     6481 ttatattttt cgtttggttg ggcttattac cgcagatgca agcttttgaa caccgtttac
     6541 caagaggttg taaaattagc gtcctgacat aatctaatac cgagggtcag ctgattgaac
     6601 accgtgtatc aagaggtctt gagcctgccg tttaactctg agccacaagg aacaatttca
     6661 aaaaacagct tctcatgttt tataaatatt gtgacttgtg gccgaggagt tggcaggaga
     6721 acaccgttta aggaagaagt tgtgaaaaag ctgtcctgca tcaagaaata aggagctgcg
     6781 acaagaattt tgtcacagcc tctttttttg tacgaatacc gagaaactgg cttttgaata
     6841 ccgtggagtg aagcggtcac cacaagactt ttatctcttc attagtttaa tcgcttacat
     6901 cgcagcggtt ttatgctgtg aaaagtggac ggaataatct ttagaaagaa taaaaaaatc
     6961 ttaagataac aactttttta ggaaattttc atttttcagg agcaaaataa gaaaaaatgt
     7021 gaatagagag aagtgatata cgtgacacaa cttaaaaaat tgtgccaaaa tcgtttagca
     7081 atcgttttga cagctgtttt ctttttctgg ctgaagacga tcattgccta ctatgcagat
     7141 ttttcattag gtgtggaagg tacgatccag tactttatct tgtggatcaa cccaattgcg
     7201 actaccttat tgttttttgg cctttctttg tatattaaaa agccaaagcc agcgttagct
     7261 attcttttga tcattgatat tttaaatacg cttttgttgt acttgaatat tattttttat
     7321 cgggaattca cagatttcat tacagtcaaa tctgtattag gcttttcgaa agtgtcgcaa
     7381 ggattgtcag gaagctcctt ttctttgatg aagccccatg atgtcatcta ctggctggat
     7441 atcgctgtgt tcattgggct actggtttgg ttgaaggtca agaaaatacc tatcaaaagc
     7501 aaccctgtcg gcaaaccgat ggctatcgct gttacctgtc tttcagggct tattttttca
     7561 ggaaatctag cgctgagcga agccaaccgt ccacaattgc tgcagcgaac atttgaccgt
     7621 tcatatattg tcaaatatct aggaatcgat gcgtatacta tttatgacgg aatcaaaaca
     7681 ggaatgacta gcagtgttcg cgcgcatgca agcagtaatg ggattgatga agttttagat
     7741 tatacaaaga aacattatgc agagccaaat cccgaaacat ttgggatcgc taaagggaaa
     7801 aatgtgatcg ttctccattt agaaagtttc cagcagtttt tgattaatat gaaggtagac
     7861 ggacaagaag tcacaccgtt tttaaatagt attttccaga atcaagcaac gatcagcttt
     7921 gacaatttct ttcatgaagt aggacaggga aagacaagtg atgctgaaaa tatgttggaa
     7981 actggcacat ttggtttacc tcaaggctca ttattcacag aacttgggtc agacaatgtc
     8041 tttcaagccg caccagcgat ccttggacaa aagcaaggct atacaagcgc tgttttccat
     8101 gggaatgtag ctagtttttg gaatagagat catgtatata aaaacttagg ttatgacaat
     8161 ttctttgatc gatcttattt tgatgaatcc gacgaaacgt taggttatgg gatattagat
     8221 aaagatttat tcagagaatc tgcccaatat cttgagcatc tacaacagcc gttttacacg
     8281 aaatttcttt ccgtaaccaa ccacacgcct tattatacgg atgacaagaa tttcgacttt
     8341 ccatctttga atactggcaa tagcacagta gataattatg ttcgaacggc acattatctg
     8401 gatcaatcat tggaacaatt ttttacgtat ttgaaaaaat ctggtattta ccaaaattca
     8461 atctttgtga tttacgggga tcattttggg atctcgaata cagataataa agatttagca
     8521 agcgctcttg gtaaagatcc agatacatgg gatgaatttg acaacgcaca gatgcaacga
     8581 gtgccattga tgatccatat gcctggttat actaaaggga cagtcaatca tgaatacggt
     8641 ggagaaatcg atgttttgcc gacattacta catctattgg gaatcgatga taaagattat
     8701 cttcattttg gtacagactt attatcttct cagcatgatc aagtagttgc ttttcgaaat
     8761 gggaactttg tgacacccaa atacaccgtc ctaggaggaa aagcttacaa taatcagaca
     8821 ggagaaatca tcgatacgaa agcagcagga atcgatgagg aaatctccaa agatcaggaa
     8881 aaagtcaaga ctgcgttaag tctttcagat aagctgaatc aggaaaatct cttgcggttt
     8941 tatgtgccaa acggatttga gacgattgat ccgaaaaaat atgactataa aaatgaagcg
     9001 gaacgtaatg aagcaatcga aaagcaaaaa ggcgaacatt cgacaagtgt cttttcaaaa
     9061 aatggtaata aaacaacaac caacttatat ccagtaacaa gaacagacgt tgaacagcaa
     9121 aaaaataatc aataaacaat caaaccaacc ttcagtagtt agacaacatt ctaatgcttg
     9181 aaggttggta ttttttatga aaaaaacatt tttttatttt tttcgtgtat ttttcctttt
     9241 taacgtatct atttattgaa tgtggaaaaa tttaaattaa aaaggagaac tattatgtat
     9301 gatggtgaat tgatcaagaa acttcgagtc aacaagaagt tgacacagtc ccaattagcg
     9361 gaaggaatct gttttaagac ttctctagtc gggattgaga caaattcagt gaaaaaaatg
     9421 tcatttgtaa cattgagatc atttttagag cgaatgaaca tgacacttgc agaatatgaa
     9481 tggatgcgaa accagctgga tgaaccaata aagttaaaga aaaaaagacg aatgctagat
     9541 cgggtacaag aagacagctt tgatccttat aaagagatca gcaataaccg taggcatttt
     9601 aagaaaacag aagatctgta ttatttggtt ttgaatcttc acatggtcga aaaaacagaa
     9661 gtatctgaga cagaatttga atttttacgt tatgaatgcc gaatcattga agaatacttg
     9721 atgcagatac cagactatgg tcaatttgaa ctatctattc tagctgattt tccttggata
     9781 ttcagtgatc ggttcattga gaacaattat ttgaaaatca aaaaaagaat gcgacgatta
     9841 gcacagttga acaatcatga gcagtatctc tttacttttt taatgaacct gacttcgttt
     9901 tatatagaaa atggtcggtt gaaaaaggca cgtagtgtga atgaagatat gaaacggagc
     9961 ttagcacata aagaaagaag tacagtgatc tatgaaacat tgatggcaga ttattatcaa
    10021 agattgattt ctgctgcttt aggggaagaa aagacgaaag attaccaaga attcttcaca
    10081 gtgctcgaat atatgttagg aaagaatcaa cggaggtcct tagagaaaaa actgctggcc
    10141 attgctgaac ccagcaacta gaccagcagt ttagaatttg tatcacttcg ccgctttttc
    10201 agacggtttg tttatatcga atggcgggaa aaagcgggcg atctctttta ccgcagcatc
    10261 acgggaatca gaagcgtgaa tgatgttttc agtccccggc agcgcgtagt ctcctcggat
    10321 cgtacctgga acagcatcgg cagcttttgt tgctccaacc atcttacgga cgatatcgat
    10381 cacttcctcg ccgaccagca caagataaac gacaggaccg gaagtcatgt aatcgatcag
    10441 ttcatcaaaa aaactgcgct cagtcaagtg ctgataatgt tcttttgcta attcaggagt
    10501 catagtttca aacttcattt cagcaatggc cagtcccttt gcttcaaaac gttggatgat
    10561 gcttccgact aaatggcgtc tcacgccgtc tggcttgata ataacaagtg ttctttccat
    10621 aacagcacct cgtttcctac tatatttcta ttatagcggg aggtggaaaa tgagacaaat
    10681 aaaaccgcgt gagacaggaa tataacgtgt gtattttaaa aaaagtatga aaaacatgga
    10741 aaattgattt gacatcctct tttttagttg ttatattaac gaaggaaaac cgaataagat
    10801 ttgttaggtg aggctcctat acaaacatag gctactgccc aaaaacgtcg agagacgcca
    10861 atgggtagaa caggaattgt cgagataagg cttttcataa ggtagctaaa agcaggtatc
    10921 tgctttttac gttgtacagt gtcaaaactc aacgatgaga tctgagaagc tttttttaat
    10981 atgctttttc aaacctttcg ctgagtattt ggcgagaggt ttttttgttg ccgcaaatcc
    11041 ccatcggtga aaaataaaaa ggaggtggtc taattggaaa aataactatt cgaaaaatga
    11101 ttggaagtca tgaattaaaa ttacttagta aaggagatga gaacaaatgg atgacagtca
    11161 ttcgaaaaac aaggatctag aggaaaggcc agggctatta tctatctgtt ctcagcccct
    11221 ttcagttttt tctctatcga tccgatgaat agctaaagac caaaagcagc gagaacagct
    11281 gtcttttgga cacaaaggag agattgagat ggaaaaaaag aaagatacac aaaaaatcta
    11341 tgcagctttt gctggaaaaa cgaaacaaga attatttgaa ctattccatg catcagcaaa
    11401 tggattgaca gaagaacaaa tcgaacgttc aagagaaaat tacggtgaaa atgcgatttc
    11461 atatggcaaa aaaacaccat tcattgtaga aatattgaaa gcatacatca cgccatttac
    11521 attagtgttg atcgcattag gaattatttc attcatcacg gaatatgtat tagcagctcc
    11581 tggagataaa gatcttttcg gtgtagccat tatcttcact atggttatcg tcagcgggac
    11641 aatgacgttg attcagtccg tacgttccaa tcaagcggct gaaaaactga aatcacttgt
    11701 gaaagtaacg gcagctgtaa aacgtaatgg aaagtacaca gagattccta tggaagaaat
    11761 cgtctgtgga gatcttgttc ggctttcagc aggggatatg ataccagcgg atatgcgttt
    11821 attgtccgca aaggatttat tcatttccca agcagcattg acaggggaaa gctatccagt
    11881 agaaaagcat gcgaaagtat gtgaagacga tcagcaaagc gaaacaagtt acgaaaatct
    11941 tgtctttatg ggaagtaatg tcatcagtgg aagtgcggaa ggaatcattg tttctgttgg
    12001 aaatgaaaca ctatttggtc atgtagcaga aacgctatct gaaaaaccaa ttcaatcaag
    12061 ctttgaatta ggcatccata aaacatccat gctactgatc aaatttatgt cattgatggc
    12121 tccagcagtt atcgtcatca atgggcttac aaaaggggat tggctagaag cttttctttt
    12181 tggactatct gtagcagttg gtctaacacc tgaaatgctt ccaatgatcg ttacgacgaa
    12241 tttggtcaaa ggagcaagtg tgatggccaa aaaaggaaca gtgatcaaaa atttgaatgc
    12301 catccagaac tttggtgcaa tcgatgtgtt atgtacggat aaaacgggga cattgacaca
    12361 agataaaatc attcttgaat atcatatgga ttgtgaagga aatgaagatg atcgtgtttt
    12421 gcgccatgcg tatctaaata gctactatca aacagggctg aaaaatttgt tagatgtggc
    12481 tatcattgat gaagcaacag aaactttaga tacagataag atcaattatc aaaaagtgga
    12541 tgaaattcct tttgattttg aacgtcgtcg tatgagtgtt gtggttgctg atccagctgg
    12601 taaaacacaa atgatcacaa aaggcgcgat tgaagaaatg ctgaacattt ctaaatttat
    12661 tgatcttggc ggtactgtta caccattaac aaaagaacga aaagaagccg tacttgcaaa
    12721 agtacagtca cttaatgaag atggcttacg agtgattggt gtcgcacaaa agaccaatcc
    12781 aagcgtcgtt ggagaatttt cagtcaaaga tgagtcagac atggttttga ttggttatct
    12841 tgctttcctt gatccaccaa aagaaacaac gaaacaagcg ttgaaagctc tgaaagaaca
    12901 tggtgtagct gtaaaagttc tgactgggga caacgaatta gttacacgtt cagtgtgccg
    12961 tcaagtaggt ctagagatca atgaattggt taccggtgag aaaatctcgg aaatgagtga
    13021 tcatgaatta gcacaagtag ctgaagacca tgaagtattc gtaaaattga atcctcagca
    13081 aaaagcacgt ttgacgacag ctttacgtaa aaatggtcat actgtcggat ttttaggaga
    13141 tggtatcaat gacgctcctg caatgaaagt ggctgatatc ggtatttctg tagatacagc
    13201 tgtggatatt gcgaaagaat ctgcagacgt gatcctttta gaaaaagatt tgacgatttt
    13261 ggaaagaggc cttctttctg gacgagaaac ttttgggaat atcatgaaat atatcaaggc
    13321 tacagcaagt tctaattttg gcaatatgtt ttccgtgctg attgcaagta cgttcctgcc
    13381 atttttacca atgctgcctt tacaactttt gtttttgaat ctgatctatg atgtttcctg
    13441 catttccttg ccatgggatc ggatggataa agaatatttg gacgagccaa aaaaatggaa
    13501 tgcatctagt attggggaat tcatggttta ctttggtcca acaagttcga tatttgatat
    13561 tactacttac ttgttgatgt attttgttat ttgtcctgct gtagtgggcg gaagtttcca
    13621 tacattggat agttcacagc gagtgatttt catcgcatta ttccatgcag gatggtttgt
    13681 cgaatcacta tggacacaaa ccttagtgct ccatgcactg cgtacaccga aagtaccatt
    13741 tatccaaagc aacgcaacat ttgccatgtt tgttatcact acattaggaa ttattgtcgg
    13801 ttctgtcctt ccttttacaa gttttggtgc tgaattaggc ttgatgccac ttcctggcaa
    13861 ttactggtta tggttatttg ctacagtcat cgcttatctg gcgcttgtga cagctgtgaa
    13921 gaaaatctat atccgtcgat tccaagaact cttataaacg agaaggctgg ttctttgctt
    13981 ctgttctttt tggaccagtc gtgctaaact atgtgtattg ttttggaaag gattaatgta
    14041 tagatgaaag gtgcaatatt tgatttagat ggtttgttag ttgacacaga aaaaacgtat
    14101 cgtgatggct ggctttgggg atttcagcaa tttgatatcc atatcccaaa agaaatcgtt
    14161 gacgcttggg gcggaaaaaa ctggaaacaa agttttgata ttttagtgaa agcagcaggt
    14221 agccctgaaa aagtacagga agtccgagcg aagcgtgaag aatattttta tcaacagctg
    14281 acaagcggag ccatccactt aaaaccatat gcaaaagaaa tattggcaga cttaaaagaa
    14341 cgtcatatga aactaggcat tgctacgacc actgttacga aaagagcaac agatatcttg
    14401 gatcacttta aattagctga ttacttggac acgtttacat tcggagacga agtgtcggag
    14461 aacaagcctt caccgattcc ttatctgaca gctttggaac gaacaggatt agaagctagt
    14521 tcagcctttg cagtggaaga ttctttagta ggagcaacag ctgcttcacg agcaggatta
    14581 ggcgttgttt tgatacctga ttccagtttt gtccgaaatt ataccgtcga agaaaaggaa
    14641 aaattgaaac ttcttgcgga aggaaaagat cttcgaactg ttat
//
LOCUS       DAXWOO010000061        14548 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.061, whole genome shotgun sequence.
ACCESSION   DAXWOO010000061 DAXWOO010000000
VERSION     DAXWOO010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.061"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            78..1052
                     /gene="bsh"
                     /locus_tag="ACGE70_001446"
     CDS             78..1052
                     /gene="bsh"
                     /locus_tag="ACGE70_001446"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFF8693090.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNINKEDLDRHELVSYPVIKEQQINYVN"
     gene            1377..3272
                     /gene="asnB"
                     /locus_tag="ACGE70_001447"
     CDS             1377..3272
                     /gene="asnB"
                     /locus_tag="ACGE70_001447"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFF8693091.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKLDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(3467..3913)
                     /locus_tag="ACGE70_001448"
     CDS             complement(3467..3913)
                     /locus_tag="ACGE70_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFF8693092.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            4141..4290
                     /gene="rpmG"
                     /locus_tag="ACGE70_001449"
     CDS             4141..4290
                     /gene="rpmG"
                     /locus_tag="ACGE70_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFF8693093.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            4378..4896
                     /locus_tag="ACGE70_001450"
     CDS             4378..4896
                     /locus_tag="ACGE70_001450"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFF8693094.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(4973..5929)
                     /locus_tag="ACGE70_001451"
     CDS             complement(4973..5929)
                     /locus_tag="ACGE70_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFF8693095.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            6095..6901
                     /locus_tag="ACGE70_001452"
     CDS             6095..6901
                     /locus_tag="ACGE70_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8693096.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKITLLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            6898..7887
                     /locus_tag="ACGE70_001453"
     CDS             6898..7887
                     /locus_tag="ACGE70_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFF8693097.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            7884..8888
                     /locus_tag="ACGE70_001454"
     CDS             7884..8888
                     /locus_tag="ACGE70_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFF8693098.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIGLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            9020..9562
                     /locus_tag="ACGE70_001455"
     CDS             9020..9562
                     /locus_tag="ACGE70_001455"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFF8693099.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            9582..10280
                     /locus_tag="ACGE70_001456"
     CDS             9582..10280
                     /locus_tag="ACGE70_001456"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFF8693100.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            10301..10513
                     /locus_tag="ACGE70_001457"
     CDS             10301..10513
                     /locus_tag="ACGE70_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFF8693101.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            10513..11475
                     /locus_tag="ACGE70_001458"
     CDS             10513..11475
                     /locus_tag="ACGE70_001458"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFF8693102.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRYLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACANLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            11493..11888
                     /locus_tag="ACGE70_001459"
     CDS             11493..11888
                     /locus_tag="ACGE70_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFF8693103.1"
                     /translation="MVLWVINIILLLIVLAIIFWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            12051..12416
                     /locus_tag="ACGE70_001460"
     CDS             12051..12416
                     /locus_tag="ACGE70_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFF8693104.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            12413..14224
                     /locus_tag="ACGE70_001461"
     CDS             12413..14224
                     /locus_tag="ACGE70_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFF8693105.1"
                     /translation="MKIGIIGAGPRGLSIAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNDLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSREGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGSMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLEIPLTYQDESFAEASRAFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPVIRTKELAALIDAGIFHLFEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(14286..14453)
                     /locus_tag="ACGE70_001462"
     CDS             complement(14286..14453)
                     /locus_tag="ACGE70_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFF8693106.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
BASE COUNT         4500 a         2455 c         3054 g         4539 t
ORIGIN      
        1 tggccttttt tttgataaaa aaaaatacta gatgtaagat aaaagtgtaa aaacgatttg
       61 ttttaggagg gaaaacgatg tgtaccgcaa tcacttatgc aacgaaagac cattattttg
      121 gaaggaattt tgattatgag atgtcttata atgaagtagt cactattact ccaagaaatt
      181 atcgtttcga ttttagaaag gtaaaaaatt tagataaaca ttatgcaatg ataggaatag
      241 cagctggggt atccaactat ccactctatt atgaggctac aaatgaaaaa gggttgagta
      301 tggcaggact gaattttcct ggcaatgctg attataaaga attacaagaa ggaaaagata
      361 acgttgcacc atttgagttt attccatgga tactagggca atgctcaacc atagacgaag
      421 ctaaagaatt attggctact atcaatctag tcaatatcga ttttagtgaa aaactaccct
      481 tgtcaccttt acactggtta ttagctgata aagaaaaatc gatagtaatt gaaagtatga
      541 aagatggact tcatttatat gataatcctg tcggtgtgtt gacaaataat ccgccatttg
      601 attatcaatt atttaatctg aacaattatc gctcgctatc aaatggaaca ccagaaaatc
      661 atttttcaaa ccagatcagc ttgaatgttt atagtcgtgg gatgggagga ttgggcttac
      721 ctggagatct ctcttctgtt tctcgatttg taaaagccac atttactaaa atgaatgctg
      781 catcaggtga ttcagaatct gaaagtatca gtcaattttt tcatatacta ggctcagtcg
      841 agcaacaaaa aggagtatgc gatactggag aaggaaaata cgaatatacg atttattctt
      901 cttgctgtaa cgttgataaa gggatttatt attatcgtac gtatgaagac agtcaaatta
      961 ctgctgtaaa tataaataaa gaagacttgg atcgtcatga attggtcagt tatccagtca
     1021 taaaagagca acagataaac tatgtcaatt aattaatgaa tagatccgtt tggtatcata
     1081 ttttctgata cagtcataat tagacacgat ctttaggtcg tgtctttttt tgctgttttt
     1141 tttggaaata gcatccacta atattttttt aacacgaaga gaataaggta gcctcaaaaa
     1201 agaaaacatg gtaaattaga tattgggact ttattttctg taaaaacaga aactttcatc
     1261 ttttgttttc acgtgacgaa attgtaagag agaaaatatt cgtccgctat ttattctatg
     1321 ataagatgca tatgaatgaa ataaacagta attttatatt aggatgtgaa agttaaatgt
     1381 gcggtattgt aggttttgtt aacgacaagg acaacaaaaa aacaattatt aatacaatga
     1441 tggatcgaat cgttcaccgt ggacctaata gttcaggaga atatatcgat aaacatgttg
     1501 ctttaggatt cagaagatta agtattattg acttagaggg cggtactcag cctatttaca
     1561 atgaagatcg gacaaagatc attattttta atggcgaaat ctataattac cagcctttga
     1621 gagaagaatt gattgcagct ggccacgttt ttcaaacaca tgcagataca gaagtccttt
     1681 tgcatggtta cgaagaatgg ggaacagaat tattgcaaaa gatccgcgga atgtttgctt
     1741 ttgctatttg ggataatgaa aagaatgagt tattcggtgc aagagaccac tttgggatca
     1801 aaccatatta ttatgcagaa atgaacggaa catttatgtt tggttcagaa atcaaaagtt
     1861 ttctgcctca tcctgatttt aacaaagaat tgaacaaaga agcgttgaaa ccttatatga
     1921 ctttccaata ttctccatta aacggcgaaa cattcttcaa agatgtctat cgtttgcctg
     1981 aaggccatta ttatacgtat aaagatggaa aactagacat tcagcaatat tgggatgcag
     2041 attttgaaac aaaagaaacg cattcccgac aagaatggat cgaaaaaatc gacgaaaccg
     2101 ttcaagcttc tattgaagca catacggtca gtgatgtaga agttggttct ttcttatcca
     2161 gcggggttga ttccagttat gtaacctctg tattgaaacc agatcattct ttctcaatcg
     2221 ggtttgatga taaaacctat aatgaagcga ttgaagccag aaaactaacc gaactattgg
     2281 acttagataa tacagcagca gtgatcgacg gagacatgtc gttcaaagca ttccctttga
     2341 tccagtatca cttagatgaa cctgattcaa atccttcttg cgtacctttg tactttttag
     2401 caaatcttgc ttcacaaagt gtgcgggtag tccaatctgg agaaggggcg gatgaactgt
     2461 ttgcgggata ccaaacgtat ggattccata cgaattcaaa attcatccgt gtgattgctc
     2521 aaggattgaa aaaattacca aaaggaacac gctataattt aggacgtaaa atcggaaaaa
     2581 tgaaaaattt ccatggacgg atccatttat atgaatcact tgctcctgca aaagaatatt
     2641 ttatcggtca tgctcgagtg ttcgaagaat cagaagcatc agaagtgtta acaccaaaat
     2701 atcaaacagc accatctgtg gatgaaatca tgactgttca ttacgagaaa acagaaggaa
     2761 tcaaagatga agtcaataag atgcaatatg tcgatcttca tcaatggatg ccaaaagata
     2821 tcttgttaaa ggcagataaa ctttctatgg caagttcttt ggaagtccga gtacctttat
     2881 tggatatcga agtcatgaaa ttggctcaac aaataccaag taagttttta ttgaatcaaa
     2941 acaacacaaa agatattttc agacaagctg cgaacaaaca tcttccggaa gaatggtcga
     3001 atcgtgtgaa attaggattc cctgtgccaa tcaaagcttg gttgaaagaa gaacacggct
     3061 acgaacaagt caaagcatta ttcgaagcag attttgcaaa agaatttttt gatcaagaaa
     3121 aaatcatgaa gttgctagat gaccatcacg aaggacgtaa agaagagcaa agaaaaatct
     3181 ggacaatctt cagtttcttg acatggtata aagtctactt tgtagatgaa acgattccgc
     3241 aagcagaagc aatcgactat gtgactgttt gatgaaaatc aaatatcaat aaaaaagagg
     3301 ttttctccta ccgtttaaca ctgagtgata agaaacaatt tcaaaaaaca gctcttcccg
     3361 tttcatagat attatgactt atggttcgga gttggcagag gaacaccatt gtttcgaaca
     3421 taagggggtt gtgacacgat tttcgtcaca acccctttac atatgttcaa taatcgattt
     3481 ctttttcttt tgtcccatct tttttataaa tgaacaaagt tgattctttg ttttgtgcta
     3541 cttgctttcc tttttcgatt gcttcttttt ttgtatcaaa atggttgctt gccttttttg
     3601 cccctttaga tttcacgatc cattgttctt cctcatattc tacgatcaca tctgaatcaa
     3661 gcagttttcc agcacgagga ttcgtatcgt gttcatccgt cttgcttgga tttttttctt
     3721 tttcgaaagt tcgtttttct gattcactag catcttcata ccattttttt gcttgatcaa
     3781 tggcaattgg gatcgcacgg ttatcagggt acccttcatc taataatgca tttgcgatgt
     3841 cgattgcttt ttttcttgtc aatttatcta agtttttcat tgatgcagga aagtccttca
     3901 tgttccatgg catattagta tcccccttta ttaaaaaaca taatctatat aaacaagtgt
     3961 aagggtattt atacaaaaag aaaaagattt tgtttgagaa gtaaaaccgt tgtatttatc
     4021 gtcaagtagt tttacttaac aaaatcgtga aaaacactag caatctagaa aaaatcatgg
     4081 taagataatc aagtctgaga gtcatcttag aggattcttg taaaaatggg agggaaaaac
     4141 atgcgcgtaa acatcacttt agagtgtact tcttgtaaag aacgtaacta tctaacaagc
     4201 aaaaacaaac gtaacaatcc tgatcgtttg gaaaaacaaa aatattgccc acgcgaacgc
     4261 aaagttactt tgcaccgtga aacaaaataa tgtttttaaa gttctcaatt ccttgctggt
     4321 caaagaattg agactttttt tatctcaaaa catctaaaat cataaggagc ggataatatg
     4381 tttatttcca tgtgggcaca agataaaaat ggtctgatcg gcaaaaatgg tttgttacca
     4441 tggcgattgc caaatgatat gcgtttcttt cgcgaacata caatggatcg aattttagtg
     4501 atgggaagaa agacctatga aggaatgggg gatctttcac ttccttatcg ccatatcatc
     4561 gtgttgacaa cgcaaccaaa ttttcgcacg aaagaaaatg cggaagtaat gcattcgatc
     4621 gatgaacttt tgtcgtatag tcaaacgatc tcagaagata tctatgtttc gggtggtagt
     4681 cggatttttc aagaacttct tccttacact ggtcggatct ggcgaacatt gatcgatagc
     4741 acgtttgaag gcgatacgta tattgggaat attgattttt cagacttcgc ccttatagag
     4801 gagtatgagg ggataacgga tcaagaaaat ctatatgccc atcgttttca aaaatgggaa
     4861 agagtaaaaa atagggttgc ttcaaaagaa gaataaaatc tatgcgtcta gtttgttaat
     4921 aagaaaaaaa gcatctcacg actaatcgtg aagattcttt ttttagttaa aatcaattta
     4981 gtttttcttt gatatcattg accatttcct gactggcaat aggtccgtta tatagccaac
     5041 tagattcagg tccgaattgc agtacatgat tgtctgtcac tgctttaaga ttcttccagc
     5101 ttggttcatt gaacatggca gcatctttgt cactgttcac aaggatgatg tagtcagcat
     5161 ctaattcagc tagcttttca gaggaaacag cagaccagtc agaagtggct tttttggaga
     5221 cctcttccac tagatcagga acaccaaatt ccagatcgtc gtatacgatt cttccacttg
     5281 accgattttt gctgaccata aaggctgagt tgttggtcac ccacaacact gcagctgtct
     5341 tgttctcgat tttatctgct agttcttctc gagttttctt gatggatttt tgataatcgg
     5401 aaatgatttt ttctgcctct ttttcttttg ccaaagcttt gccgacatct tttagttgtt
     5461 cttcccatgt gacatctgtt ccgtttttta caacataagt tggcgcgatt ttgctatatt
     5521 gttcgtattt cccgccttcg acagtagcgg aagaagaaat caacagcaaa tcaggctcaa
     5581 agcttaacac tttctcataa ggaaggtcat aagaaatcgt tgggacatcc tttaaatcat
     5641 cttgcagata atcttgaatg gaaccactgc caacagtcca ctgggcgata ggtttttccc
     5701 ctagtgcaat cagatagtct tctaagtaac tgccgatgat ccgtttgggc gcgtttggaa
     5761 tctctacttt atgtccaagt gtatctgtga ctgtatgagt actattggat gaagtcgtcg
     5821 aattggcagt tgtagctgtt tctttatctg aagaggggga gcaagcgctc agtactccag
     5881 ccagcagcaa tatgctagat aatgtgaata cttttgtttt taatttcatt tctttcctcc
     5941 taacataatt gagaatcttt ctcattggtt attattcctg atatttcgaa cgctgtcaac
     6001 cgcttcattt ggctatagat atgaaagtag cgcttgacga aatgttgaaa cagcgtcata
     6061 attgagaatg attttcagcg aaaggatggg tgaaatgacg agattaagag cagctgacat
     6121 tgcagccggc tacgaaaaca aaaaaatcat tgaaaacatg tctgttacga ttcctgaagg
     6181 gaaaatcact agtttgattg ggccaaatgg aagtggaaaa tctactttat tgaaaagttt
     6241 tacccgtata ctacctgtga aatcaggaaa gatcattgtc aataactctg atatctctac
     6301 atacagttca aaagaacttg caaaaaaaat tacattatta gcacaaacta gtgaacaccc
     6361 tacaggtatg acagtagaag aagtagtatc ttacggacga tacccttatc aaaaactgtt
     6421 cagtggcatg gacacgcaag atcatgaagc aatcgagtgg gcgttggatg ctacaggtct
     6481 agctggattt cgtatgcggg atatcgcaac gctgtctggt ggacaaaccc agcgtgtctg
     6541 gatcgctatg gcgcttgctc aggaaacaga tatcttgatc ttggatgaac cgacaacatt
     6601 tttagatccg gcacaccaat tagagatcct acagttattg actgaaatca atcaaatgaa
     6661 acaaacaacg atcttgatgt cgattcatga cattaatcat gcttcacgtt tttcagatta
     6721 tttattagca atgaaaaacg gtaagttgat cacagaaggt acacctgaag aagtgatcac
     6781 aaaagagtgt atgaaaaaaa tatttgagat tgatgcaacg attgtcacgc ttccaagaac
     6841 aaataaacca gtttttctaa cctatgattt gttgccaaaa gagcaggagg atcaattatg
     6901 aggactatga agaaaaatag actcctgctt ttggtctgtt ttgttctttt gattattttt
     6961 tttgttgctc tttcaaatgg cgcagcgact gtgggaaaag cagatttttg gcaggcgttg
     7021 tttgattttg atagatcaaa acaaagtcac caaatcatac gaaatattcg tttgcctcgt
     7081 gtggcgggag catttcttgt agggagttgt tttgcattga gcggtgcatt gatgcaagga
     7141 gtgacaagaa atcctttagc agattcaggc ttactaggaa tcaacgcggg tgcttcgttg
     7201 gcaatggccc ttagttttgc tttttggcct aaagcttctg atttttctgt gtttctaatg
     7261 tcattggcag gttcttttgc tgtaactttg ctcgtatttg gttttttgca gttttcgaaa
     7321 atgggcatga atcctttaca gctgatttta gctggcgtag ctattagttc tttttttact
     7381 gctatcagcc aagcacttac ccaattcttt aatctgcagc aggatcttgc tttttggttt
     7441 gtaggcggag ctgccaatat cacatggaaa caattagcag tattgtttcc tttatatata
     7501 cttgctgttt tggtttcttt ttcattagga aaagcaatat ctttgattca tttgagtgaa
     7561 acacatgcat tagctcttgg aagtcatcct aaacgaacgc ggttgattgt tttctgtcta
     7621 gttgcagtgt tagcagccat tgccgtttcg ttagtcggac ctgtttcatt tattgggctg
     7681 ttggttccgc cagttgttcg cggaatggtt ggaacggact atcgttatgt tctacctgtc
     7741 tcttttttag ttggcggtgg attggttatg ttggcggatt ttacagcgag gatgatcaat
     7801 cccccttttg aaacaccttt tggattagtc attgcattga ttggcgttcc gtttttactg
     7861 atacaagtaa ggagggaaca acggtgaaaa aaatatattg gacgttctat ttcttgcttt
     7921 ttgtacttgt catcctgtct ataggtagtt taatgattgg tacgcctttt atttacctgg
     7981 atcagctttt tgccacgttc atagggaaag aatcatcttc tttggcgctg attgttagag
     8041 aatttcgagt tcctcgatta ctgatcagtt tattagcagg agcttgtctt ggcgtttctg
     8101 gttatatctt gcaaggagta acaagaaatg aattggcaga ttctagtata ttgggcatta
     8161 atgccggagc aggattttta gtgatgcttt atttaggttt cttttcgcac tattcgctgc
     8221 cgtttctatt gctagttgta gcctttctag gtggtgtttt agctgcttgc tgtgtctatc
     8281 ttgcggccta tgatcgaaat ggctttttgg gtatgaatcg aatcttattg tccggtattg
     8341 ccgttaatgc cggcctttct gcattaacat tgctatgcac gatccaatta tctaaggaaa
     8401 attatggatt tgtgaatgca tggttagcgg gatcaatctg gggtgcgagc tggccttatg
     8461 tttgggcatt gcttccatgg gcggttatct taattccgct aggaggactt tatgcacaga
     8521 ggattggatt gctatcattt ggtcaagaac gcgcgcaagg attaggcctt aacgtggcta
     8581 aaagtcaaaa aatccttttg ttgttagctg ttgctttggc aagcggtagt gtagcggtga
     8641 caggtagttt atcttttgta ggactactag ctcctcatgc tgcgaagcta gtcgtacgta
     8701 aagaaaataa ctggaccttt gttctgagcg gtttgttcgg ttccattttt ctgcttagtg
     8761 cagatatggt tgctagagtg attctaccaa gtggggaagt acctacagga attttgattg
     8821 ccctttttgg tgcgccatat tttctgtatg cactttttat cagacaaaaa cacgcgctga
     8881 acagttgaaa tttattaaaa aaggaaggcc aatcatttct aggtcttctt tttattctat
     8941 ttatgcattt atttgaaagt tcgattgcta tttttctttt tttcctatac cattttatga
     9001 gagaaggaag ggtgagtttt tggaccaatt gaaagtaaga ttacgccgga aaggaatcga
     9061 aaagctgaaa aatctggcca aaagtggcaa gaaaaaggaa aaagaacagc gaatctataa
     9121 acagctattc gccagcgagt actggaagtc agcaaaagtg atagggacga ctttatcgaa
     9181 tgaatttgaa ctggatacac agccggttat ccaacgagca gaaaaagaac aaaagaaaat
     9241 agttgtacct aaaactttgc cgaaacgtca gatggcgttt tatgaaatgg atgaacagtc
     9301 cgttcttgta cgttcgagtt ttggtgttct tgagccacaa tcagagaagc tctatttgcc
     9361 ggagatgatc gatctcctca ttgtaccagg tattatctac aatagagccg gttatcgaat
     9421 cggttttggc ggtggttatt acgaccggta tttaaataat tttcataaaa aaacatgtag
     9481 ccttgttttt cgtgaacaat tgaatgaaca atggcaaccg gagagctttg atcagaagat
     9541 ccaacaattg tttattgact aaaatgaaat gggaggtttt catgaattat caacagcaaa
     9601 taaaattgcg tgcatggtta agacgaccgt tatggaccta tgctttttta gggatacaga
     9661 cagcggtctt cctcatgatg gaattatttc cgcgacttga gatcccttat tatgccggaa
     9721 tgtatggtcc ttatcttgtt cacttcaatg aatggtggcg tttagtgaca ccaatcttca
     9781 tccattttgg actgatgcat ttcgtaatga actcactgat actgtatttt atggggcagc
     9841 aaatcgaggc aatctatggc cattggcgtt ttttcttgat ttatatgttc agcgggataa
     9901 tgggaaatac agcaagcttt gcttttaacg aagcgaatgt tctgtctggt ggtgcgagta
     9961 cttcgatatt cggtttgttc ggtgcgttgt ttatcttagg gtttcatttt aaacataacc
    10021 cagcgatcca gcagctagtc agacattttc ttttatttat tgtgttgaca tttgtttttg
    10081 gtcttctgga tacctctgta gatgtttggg gacatgtcgg tgggctaatt ggcggattag
    10141 tgttagggaa tgttttgggt ctaccgaaac aacagacaag ctattcgata caccaaagga
    10201 ttctttctac actagttttt gtatttcttt ttgtaatatg tattttactg ggtttaaaaa
    10261 aatatggttt acttgtataa tatgaaggaa gtgcttaaag atggagagct tatatgatgt
    10321 acagcagctg ttcaaaaaat ttggcatctt tatctatgtg ggtgcacgca tctatgacat
    10381 tgaattaatg actatcgaat tgcgtaatct gtatgatagc cgtttgattg atcatgatac
    10441 atacatgaca gcgtggcgca tattaaaaag agagcaccga attgaagaaa gccgaaagaa
    10501 aggaaatttg taatggacaa gaagattatt gggattgatt taggtggaac aacagcaaaa
    10561 tttgcaattt taacaccaga aggagagatt caacaaaaat ggagcatcga taccaatatt
    10621 ttggatgatg gtaagcatat cgttcctgaa atcattgaat caatcaacca tcgtttgaat
    10681 ttatataata tgaaagccga agactttatc ggaatcggca tgggtacccc aggaagcgtg
    10741 gacagcgaag ctggaacggt tattggtgcg tataacttaa actggacaga agttcaattt
    10801 gtgaaaaaac tgatcgaggc aggaacagga atcaaattcg ctatcgataa tgatgcgaat
    10861 gttgctgctt taggtgaacg atggaaaggt gctggagaaa acgatccaga cgttgtattc
    10921 gttactctag gaacaggtgt tggcggtggt atcgttgctg gcggaaattt gattcacggc
    10981 gtgagcggag caggcggaga gatcggccat atcactgtgg atcctgaagg gttcgaatgt
    11041 acttgcggta aacgcggatg tttagaaact gtgtctagcg ctactggtgt ggtacgtcta
    11101 ggtcgttact tggcagaaga atacgcagga gattcaaaac tgaaagcaat gttagacaac
    11161 ggtgaagaag taacaagtaa agatatcttt gaaatggctc aagaagacga tccgtttgca
    11221 ttgatggttg ttgatcgcgt atgcttctac ttaggtttag cttgcgccaa cttggggaat
    11281 acattgaatc cgtcaagtat tgtgttaggt ggaggagtat cggcagcagg agaattttta
    11341 cgtagccgtg tagagaaata ttttaaagaa tttactttcc cgcaagtaag agaatcaaca
    11401 aaaatcaaat tagcagagct tggtaatgaa gctggtgtca ttggtgctgc ctcattagcc
    11461 ttgaaatttg cttaaaaaaa cggaggcatt acatggtttt atgggtaatt aatatcattt
    11521 tgctattgat tgtgcttgca atcatctttt gggaagtcta tctacgagta atggccaagc
    11581 gttcagcaac gactttgaca gaagaagaat ttcgagaagg aatgagaaaa gcacaagtaa
    11641 tcgatgttcg cgaaaaagat gtgtttgatg ctgggcatat tttaggtgca agaaacatcc
    11701 cttacactgt tttgaaagat tcattaggat ctattcgaaa agaccagcct gtctatattt
    11761 acgatcaaaa gaaaagcttg agcatccgta cagcaaataa attacgtaaa gcaggttatc
    11821 aagagattta tttacttaag ggtggatatg acggatggtc tggaaaaatc aaaagtaaaa
    11881 aagtataaaa cagatgattg tatgacagct ttaaaaaccg aatcaatcat tagattttga
    11941 ccaagatcac atgaacggcg caatggatag ttgtgccgtt catttttttt attttgtgct
    12001 attatctaca tatataagct aagcgaaaag gcagaaagaa ggaaacaact atgatcaaat
    12061 taaaacgtgt ttaccttgat gctgaaaaag aggatggcta tagaatactc gtggataggt
    12121 tctggccacg aggaatttca aaagaaaaag cgcagttaga tctgtggttg aaagaaattg
    12181 cacctagtaa tgaagcacgc aaaacttttg accatgtacc agaacgattt gcttcatttg
    12241 caaagaaata ccagcaagaa ttgattttcg aagaagaaaa gaagcaagct atttttaaac
    12301 tcagtcaact tatgaaagaa catccagtca ttacttttgt ttatggtgca aaaaatgaac
    12361 atcagaacca tgctgtttta ttgaaaaaat ttatcgaaat ggaggcatgc aaatgaaaat
    12421 agggatcatt ggtgccggac ctagaggatt aagtatagca gaaaggattc tccataataa
    12481 tcaggacaga cattctcttg aactttttct tttcgatccc aatggtcctg gaggtcgagt
    12541 ttggcggttg aatcagccac cggagttatt aatgaattct gtttctcaac aagtgacgct
    12601 ttttacagat gaaacattgt ccagtggagg attggtaaca cagggaccca acttgtttca
    12661 atggagtaaa ggacctgcta cagattatat cgaacagacg gtaaccaaaa ataagcaact
    12721 aattatagaa gaaatcaatc gtctggaaaa agatcagcaa tctactcgat gtttatacgg
    12781 actctatcaa cgctggtttt tctcaaagtt gcaagaagaa tttccagctt ctattcagtt
    12841 tgtttattct ttagtaaaaa aagtcagcaa aagaaaagat ggttatcttt tacagacaga
    12901 tcagcaaagc tattctatcg atcagcttgt tttagcaagc ggtcactggg aaaatgatga
    12961 gacagaagaa gaaagagtgt gggtagacta cgcaaagaaa aatgatttac tgtatcagct
    13021 tccttctaat cctgcagatg tcgatctttc gaagataccg cctaaagaaa atgtcttttt
    13081 aagaggtcta ggcttaagtt tttttgatta tgtcggattg tttacagtat caagagaagg
    13141 gcgcttcgag aacgaaaaag gaaaacttgt ctatcatcca tcaggcaagg aaccgattgt
    13201 atacagtgga tcaagaaggg ggctccctta ttaccctcga ggaagaaacc aaaagcaagg
    13261 cggatcaatg gcatggccac ggttactaac aaaagaaaac ctggagcaat ggcatcagtc
    13321 gggatttttg actggcgaaa cattttttga ctatctgaaa aaagacgttg agttgtttta
    13381 ttacaagaaa atcatcgaag aatatcattt gccgattcca aagaaaaatt ttgagcatga
    13441 ttttctctcc ctctcttcag aggagtgtct cggaaagcat ccagcactaa tcccttatag
    13501 atgggattgg tcgtatttag aaattcctct tacctatcag gatgagtcct ttgcagaagc
    13561 tagccgagca tttattgaag ggcagataaa agaagcggaa aaaggcaatt gcacaggagc
    13621 atttgcttca acatttgatg cattaaaaga ctggcgtgat ccaatccggc aggctattga
    13681 atggcacgtt ttttctgcaa aggaatacaa agaaatacta tggggatggt ttactcatct
    13741 aaatagcttt ttaacgatcg gtccgccagt cattcggacg aaagaattag cagcactgat
    13801 agatgcgggt atttttcatt tgttcgaacc gccatttgat attcagcaaa agcacaaccg
    13861 ctatgttatt tctctacaag ataaagaaat cagcagtcgc tatttgattg aagctcgatt
    13921 gccacaaaca agcttttctc gttcaagtaa tcctgtcata aaaaatttga aggaaaacaa
    13981 acttgttcgc cctttttcat tttcagataa tacaggaagt tatcattcag aagctataga
    14041 tgttgagcga ggtacgaatc gcgtgattga tgcttcaggg gaaatccagt ctgactttta
    14101 ttgctatgga atacctgttg aaggtgtgga ttggctaact gcagcagtag ctagacctta
    14161 tacagatcca tggaatctga gacaggcaga taaaatagca caacttatat tgactgacaa
    14221 ataaaaatac gcatgatgac ttatctgttc tttcacaagt cttcatgcgt gtttttaaat
    14281 aattattaac gagaaacgcg tttgcgcatt gcttttttac ggttttcttc gatcattgct
    14341 tctttttcgt caattggatc gatcactgtt tttttcacag aagcgaccaa accagcaacg
    14401 gcagcggctg ttgccagact tcctactaat attccagata caaatttttt cattgaatcc
    14461 acctcattta ttatttgtta tatctattat ggaacaaaag agaaaaaaag gctagtttta
    14521 cgtttgtttg taaacctttg caaaaaat
//
LOCUS       DAXWOO010000062        14542 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.062, whole genome shotgun sequence.
ACCESSION   DAXWOO010000062 DAXWOO010000000
VERSION     DAXWOO010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14542
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.062"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            68..952
                     /locus_tag="ACGE70_001463"
     CDS             68..952
                     /locus_tag="ACGE70_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFF8693107.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            1066..1302
                     /locus_tag="ACGE70_001464"
     CDS             1066..1302
                     /locus_tag="ACGE70_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFF8693108.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            1390..1470
                     /locus_tag="ACGE70_001465"
     tRNA            1390..1470
                     /locus_tag="ACGE70_001465"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1424..1426,aa:Tyr,seq:gta)
     gene            1477..1548
                     /locus_tag="ACGE70_001466"
     tRNA            1477..1548
                     /locus_tag="ACGE70_001466"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1509..1511,aa:Gln,seq:ttg)
     gene            1673..1843
                     /locus_tag="ACGE70_001467"
     CDS             1673..1843
                     /locus_tag="ACGE70_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693109.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVRVKSKRP
                     TNHTKKWMAKNR"
     gene            2063..2770
                     /locus_tag="ACGE70_001468"
     CDS             2063..2770
                     /locus_tag="ACGE70_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFF8693110.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(2829..3473)
                     /locus_tag="ACGE70_001469"
     CDS             complement(2829..3473)
                     /locus_tag="ACGE70_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFF8693111.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDEAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGIPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            3599..3886
                     /locus_tag="ACGE70_001470"
     CDS             3599..3886
                     /locus_tag="ACGE70_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693112.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            3994..4314
                     /locus_tag="ACGE70_001471"
     CDS             3994..4314
                     /locus_tag="ACGE70_001471"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFF8693113.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            4395..5708
                     /locus_tag="ACGE70_001472"
     CDS             4395..5708
                     /locus_tag="ACGE70_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFF8693114.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLSNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKIL"
     gene            5705..6424
                     /locus_tag="ACGE70_001473"
     CDS             5705..6424
                     /locus_tag="ACGE70_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFF8693115.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVNSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQIAQPKIIRTISLAR"
     gene            6552..7103
                     /gene="raiA"
                     /locus_tag="ACGE70_001474"
     CDS             6552..7103
                     /gene="raiA"
                     /locus_tag="ACGE70_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFF8693116.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTANAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            7325..9859
                     /gene="secA"
                     /locus_tag="ACGE70_001475"
     CDS             7325..9859
                     /gene="secA"
                     /locus_tag="ACGE70_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFF8693117.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            9985..11086
                     /gene="prfB"
                     /locus_tag="ACGE70_001476"
     CDS             join(9985..10056,10058..11086)
                     /gene="prfB"
                     /locus_tag="ACGE70_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFF8693118.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            11191..11877
                     /gene="ftsE"
                     /locus_tag="ACGE70_001477"
     CDS             11191..11877
                     /gene="ftsE"
                     /locus_tag="ACGE70_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFF8693119.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            11870..12754
                     /gene="ftsX"
                     /locus_tag="ACGE70_001478"
     CDS             11870..12754
                     /gene="ftsX"
                     /locus_tag="ACGE70_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFF8693120.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            12906..14354
                     /gene="cls"
                     /locus_tag="ACGE70_001479"
     CDS             12906..14354
                     /gene="cls"
                     /locus_tag="ACGE70_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFF8693121.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
BASE COUNT         4858 a         2469 c         3180 g         4035 t
ORIGIN      
        1 aaaatgcttt caaatgctaa atagatacat tacaatagct ttagcaaaca aaaaggagaa
       61 tatttttatg ggaatattag tttttgattt tggtggatcg gctgtcaaat atggttgttg
      121 ggatggcaaa gagattaaag gtaagggaca atttgcaacg cctgaatctt gggaagaaat
      181 gaaagcgcaa ttatttcaag tttataaaaa aatactcgtt tcttttgaag tcgaaggaat
      241 tggaatcagc tctcctggag tagtgaataa tgagaaacag gtgattgagg gaatcagtgc
      301 gattccttat attcatggat ttaatatttt ccgagatttg gaaactctgt ttcaacttcc
      361 agtaacgatt gaaaatgacg ccaactgtgc cggaatggct gaattttatg aaggtgctgc
      421 ccgcgattat caagatgttg cttttgttgt ggcaggaact gggattggcg gggcgttgtt
      481 cacgaaagga caaatcaata aaggggcaca tctttacggt ggagaatttg ggttgatgtt
      541 cttaaatggc gataagacat tcagtaaact tggtacagcg gtacagatgg cttggcgtta
      601 ttgtgaacgc aaaggtctcg ataaaaatgc ttataccgga aaagatgtct ttgaattagc
      661 agaaactgga gatgcaatag caaaagaaga agttgagagt ttttatacgt atcttacaaa
      721 gggattattc agtattcaat tttcgtttga tccagaagtg atcgtattag gcggcggggt
      781 ttctgcaaaa gaagggctca ttgatgagat caaaagccga atgaagaagc tgacagaaca
      841 attcgatctg catgatttcg aaccgcagat tttattatgt gaatacagaa acgatgctaa
      901 tcttgtaggt gcagcagcaa actatctcgc tgtacgtcaa ggggagaatt aagagcttta
      961 gagaaggtgc agcttgcata aaagaagtga atgaaatgaa taggagattt ttacctaagg
     1021 aatgttttat ttgctgcagt atgataaaat atctgagagg tgaaaatgat gtcaaaaaaa
     1081 aataaagata ttgaagtgcg tatagaagaa gtgaaaaaag atatcaaagg taagaattat
     1141 gaagtcactc agttatttat agggaaaaaa atgattggtg agatcttagc ctatggacca
     1201 aaagaatatg aaattttctt tggtgaagaa gattttggaa aagaaaaaag tcttgaaaat
     1261 gcgatagaaa cagtgattcg acactggaat ctgcacgaat aaaaaaatat caaaaaaact
     1321 ttcaataaag ggttgcaaaa atctggaaaa ttaggtatta taactaagtc aggttgatac
     1381 aaacctttcg gaggagtagc gaagtggcta aacgcgacgg actgtaaatc cgttccttag
     1441 ggttcagtgg ttcgaatcca ctctcctcca tttcattggg gtatagccaa gcggtaaggc
     1501 aagggacttt gactccctca tgcgttggtt cgaatccagc taccccagta taccacaatc
     1561 gtaagctctc gttagttcga gagctctttt ttttgctttg ctaccttgtt ccttgaagat
     1621 tctgctgatt taagatagag ttaagttaac tacaagaatt ggagggatcc acatgaaatg
     1681 gaaagaaaaa gtagacgaag cagctgaaaa gttatatgat ttggtcaaat cagaaaaata
     1741 caatattgaa gtgaatattc cgaaaaaagg cggaaaagcg gtccgggtaa aatcaaaacg
     1801 cccaacgaat catacaaaaa aatggatggc taaaaaccgc taataataac aaaagatcca
     1861 taagtcggca ttttcatcga ttttagggtc ttttgttatt ttctaggttg gacatatcta
     1921 aaaaattgtt tctgacaata ggagtaggaa gaacgatgtg tgtctaaatc ctctgttgat
     1981 ggatagaaac ctttatcttt ttcagttgtg aatcaggtat ataccagtta taatagaaga
     2041 aacgaaagga cgggtgtacg ttatgtcgaa tcaattacct gtatatatac agattcatga
     2101 ccaattaaaa aatgaaatcg aacaaggtat atggaaagtc ggagaccgtc ttccttcaga
     2161 aagagaatta gcagtgaaat tcaatgtcag ccgtatgacg ttgagacaag caattcaaaa
     2221 cttggcagat gaaggaatct tagaaagaaa gatcggttcg ggtacatatg ttgcacgaga
     2281 aaaagtacaa gaaaaaatgt ctggtgcaac aagttttaca gaaattatgg aatcacaaaa
     2341 tcgagtaccg tctagtcgga caatctctta tttcttgact tcgccaagct caagtgaaat
     2401 ggaaaaacta aacttgagta aagatgacac gatcttgcga atggaacgta ttcgtttcgc
     2461 agatgatgtc cctatttgtt ttgaagttgc cagtgtgcca caaaagctga tcgatggcta
     2521 cagcaaagca gagattactg aatcgttgta tcgcacgcta gaagaaaaag gcgggaaaaa
     2581 gatcggtcca gcgaatcaaa cgattactgc tgtgttggct tctgagaaaa ttgctgacta
     2641 tttagaggta aaacgaggag atgcagttct tcgtttgcgc cagatttctt attttgaaga
     2701 cggtacgccg tttgagtatg tgcgtacgca atacgtagga aatcgttttg agttttattt
     2761 ggaaaaataa aaaacaggtg atccctgatt ccgtattaga aataaaagag aatcagaatc
     2821 acctgttttt atatatgatc agttaccagt tgctcgtgat aagaaacagg accctcttca
     2881 aattggactt gtccgttcag cagctcaatg atggattctt taaagtggtc agcacttttt
     2941 tcatctacag cacagatgac gctaacttga tcagtatatt ggatttcttt tataaaaaaa
     3001 tgttggagtt caagataatt ctgtaatttt cctagttgcg gatacgtgat catcacagag
     3061 atctcctgtt gcaaggtacc ttctacgatt ccaatgtcag acaatgcttg agagaccgca
     3121 tgagtgtatg ctcgtattaa tccgcctgtg ccgagtttcg ttcctccaaa atatcgagtg
     3181 actactgcag ctacattgat cagttgattt tttttgagga cttccaacat cggtatgccc
     3241 gccgttccac ttggttctcc atcatcgctg cttcgctgga tttcattttt ttctccaatg
     3301 atgaaggcgc tgcagttatg gttcgctttc caatgttctt tttttatttc ttgaatgaaa
     3361 ctttttgctt cgtcctctga ttctattcgt ctaagagaac agataaagcg tgattttttg
     3421 atctcgatct cactttgtcc attttctttt attgtacgat agcgctcgat catcttgtca
     3481 cctctttttt cattttcttt attcagtata aataaagttc taggttgttt caacactgta
     3541 acggttgcgc aattgctgta gtatgcgata ataaagacaa aggcaggtgg atctggggat
     3601 gcaggaacga atgaaaaagt acgatgcaat tacgcattat ttgaagaaca atggcggctc
     3661 tcaagtaacg ttgaccttta cccaatttga cgaactgctg tttcccagta acggacttcc
     3721 caaaacagca agggaaagta cagattggtg ggcaaatgat tataaacacc ctgaaaaagg
     3781 tgcctatggc tggataaatg caggctatga agtagttgtc atcaatttgg acaaagaata
     3841 tgtcgtgttc aataaattag tgaaatctag ttggctattt gattaaataa accgcgttta
     3901 gaaaacgttg acaaatttct ttctttttat ataatagagt aacagacgcg aagaaaacgt
     3961 ttgcgtagtt tgttttaaaa aggagagaaa agtatggcta aaaaaacaat catgcttgta
     4021 tgttcggctg gtatgagcac aagtttacta gtaacaaaaa tgcaaaaagc tgctgaagca
     4081 aaaggaatcg actctgatat tttcgctgtg tccgcttctg acgcagataa caatcttgca
     4141 aataaagacg tggatgtgtt gttattaggc ccacaagttc gctttatgaa agctgatttt
     4201 gagaaacgtc tggaaccaaa aggcattcct ttagatgtca tcaatatggc tgattatggt
     4261 atgatgaatg gcgaaaaagt gttagatcaa gcaatcacgc tcataggtga ataaatagaa
     4321 cagtataact atcgaacaag cgatccaaaa aatagatcgc ttgttttttc gtatattttt
     4381 ttagggcgaa taatatgaat catttttggg gtagaaggct tctgacgaga gatattaaag
     4441 caatagactg tgaggaggta aatttaccaa attatgagaa gatcgcagca gtggaacgtt
     4501 tgagcaactg gattctttgt catcgctgtg gaatgagaac gtccgtcatt gatggacagc
     4561 ttgcagatta cggttatttt tgtacacatt gtctatattt agggcgatgc gacactcaac
     4621 aagatttata tttgtttgat cagcctgaag caaaacctcg agaagtcgtc tttttttgga
     4681 caggacagct tactgaaaaa caaacggaaa tctcaaagag aatactttgc cattcagaaa
     4741 aaagacatca tcttatttgg gctgtaacag gtgcagggaa aacagagatg ctttatccta
     4801 ttttagtgga aactttaaaa gctggaggaa gagtagcgat ttgtacaccg cgagtcgatg
     4861 tttgtaatga attgttttta cgctatcgtt atgtttttcc agaagaaaaa atcacgttgc
     4921 tccatgggaa tacagcaacg gcttatacct ttacttcttt tgttatctgt acgattcatc
     4981 agctataccg tttttatcgg tcctttgatt taatagtgat cgatgagatc gatgcgtttc
     5041 cttataacgg ggatgcagga ttagctcatg ctgtgcaaac agcgattaaa ccagacggac
     5101 gattcatcta cttaagtgcc acacctgata aaaagttact aaaggagatc aagcagacgt
     5161 ttgacatcca caaacttgct ttacgctttc atcggcgttt gcttcctgaa cccgtcttgg
     5221 ttttcatgaa taattggcga caaaaatgca agaacaagaa gaagataaaa tctttggtgc
     5281 ggatcattca tcaattaatt aaagaaaacc atatcctttt attttgtcca agtatcacga
     5341 tgatgcatcg tttgaaagcc acacttcaag aggctcttcc gagatatgag atcacagatg
     5401 tttcttcaag agacaatcag cgggcggaaa aagtccagaa aatgcgagaa gcaaaatatg
     5461 acatactgtt aaccacgatg atccttgaac gtggagtcac atttgaaaga atatccgtta
     5521 tcgttttagg agcggatcat ccggttttta ctaaatcttc tttggtacaa atcgcaggaa
     5581 gagccgatcg taaagggcct tatacgaaca gtcaagtata ttttttctat gaagaaaaaa
     5641 ctgcagctat tcgaaaagct tgtcaggaaa tcaaagaaat gaatgaagaa gggaagaaga
     5701 tactgtgagg tgcagttgct gcagtaaaga tatcgtccaa aatttaacat tcagcgagat
     5761 tattggtgtt tcgaaataca gattacctca gacttgttca gtttgtcgcg cacaatttca
     5821 tttattggaa acaagaggct gttctggctg tcaaaaacca atccatttca atctagagag
     5881 tacgaaaaag aaagtaaatt ctatccccgt ttactgcagt gactgtcaag aatggaagaa
     5941 gaaatatccg acctatgatt ttcagcaccg tgcctttttt gtgtacgatg aaaatatgaa
     6001 ggcctggctc aatcaataca agtttatggg agatatccga ctagcaggca cctttgcata
     6061 taaatggacg cagataaaaa aagaataccc gacatacatc tattgtccaa tacctttatc
     6121 tgaaaaaagg cgaacagaaa gaggattcaa tcaagtaagc gagatgttga aagcagcaag
     6181 aattcctttt cagatgctac tcaaaagaga gaagcatctg ctggctcaag caaaaaacac
     6241 gcgaaaggaa agattggaga tgcctcaacc ttttgaactt gcagtcacct tttctaagat
     6301 acaaaataaa aaaatattga ttattgatga tgtctataca acaggacaga ctcttttcca
     6361 cgcagcagat tgtttgcaga tcgcacagcc gaaaatcatc cgaactattt ctttggcgag
     6421 atgaattaga tgtggaaatt tgatagcgat ttaatttgca aataacctag agttcgatat
     6481 aatagaatta agaagaggga aagagtggtc cttctccttc ttgattgtgg tagacgaaag
     6541 gggtaatttt tatgtttaga tataatgtac gcggcgaaaa tatcgaagta actgaagcga
     6601 ttcgcgacta cgttgagaaa aaagtaggca aactagaacg ctatttcagt gacgcacccg
     6661 atgcaacggc gcatgtgaac ttaaaagttt atacagaaaa aacagcaaaa gtggaagtaa
     6721 cgatcccatt accttatctt gtattacgtg ccgaagaaac ttctccagat ttatacgcaa
     6781 gtatcgattt ggttgttgac aaattggaac gccaaatccg caaattcaaa acaaaaatta
     6841 atcgtaaatc ccgtgaaacg ggcatgaata ctgcaaatgc tgctgcttta ttcaatgaag
     6901 aggacgaaaa cgaaaatgat tctgaattag atatcgtacg tacgaaacgc ctttcattga
     6961 aaccaatgga tagtgaagaa gcagtcttgc aaatgaacat gctgggacat aacttcttta
     7021 tttttgaaga tgcagaaaca aatggtacaa gtatcgttta ccgccgtaaa gatggaaaat
     7081 acggactgat tgaaacagac tgatcagcct tacttaaata acaacgaaaa cttgcagcag
     7141 ctactgacag ctgccgcaag tttttttgag tttcctcctc ttttagatga aatatgaata
     7201 aattatgaag ttcttacttt aatacgctta agatatttca attatctata actaatgata
     7261 aaatgaaaag taaagtctac atagtctgga taatacacag aaggaaaagg agattacaca
     7321 acgcatggcc aatttgattc gttcacttat cgaaaacgat aagaaagaac taaaacgttt
     7381 agatagcatt gcaaaaaaag ttgaagcata cgctgaccaa atggcagcat taactgatga
     7441 acaattacag gcaaaaactg ctgaattcaa gggcagatac caaaaaggag aaacattaga
     7501 tcaattactt ccggaagctt ttgcagtcgt ccgagaagca gcaaaacgtg tattaggttt
     7561 atatccatac cacgtacaat tgatgggtgg aatcgtgctt cacgatggaa atatccctga
     7621 aatgcgtact ggtgaaggta aaaccttgac cgcaacgatg ccagtatatc taaatgctct
     7681 gactggcgaa ggggtacacg tagttaccgt caatgaatat ttagcaaccc gtgactcgac
     7741 ggaaatgggc gaactttata atttcttagg attaacagtg gggttgaata tcaactcaaa
     7801 atcttcggat gaaaaacgcg aggcttataa ttgtgatatc acttatagta cgaataatga
     7861 attaggattt gactacttga gagacaatat ggtcgtttac cgccatcaaa tggtacaacg
     7921 tccgctgaat tatgccattg tcgatgaagt tgactcaatc ttgattgatg aagcgagaac
     7981 gccgctgatc atttcagggc aagcagaaaa atccacagct ttatatacaa gagcagacaa
     8041 cttcgtgaaa cgtttgaaag aagaagaaga ttataaaatc gatgtacaat caaaaacaat
     8101 cagtttaacc gaagcaggga tcgaaaaagc agaagaaaca ttcggtttgg ataacttata
     8161 cgacattgaa aatactgcat tgacccatca cttggatcaa gcattgagag ccaactatat
     8221 catgctttta gatatcgatt atgttgtcca agacggaaaa gtactgattg ttgaccagtt
     8281 cactggtcgg atcatggatg gacgccgtta ttcagatgga cttcaccaag caatcgaagc
     8341 taaagaaggc gtagagatcg aagacgaaac gaaaaccatg gcgacgatca ctttccagaa
     8401 ctatttccgt atgtacaaga aacttgcggg aatgactggt acagctaaaa cagaagaaga
     8461 agaattcaga gaaatttata acatccaagt ataccagatt ccgacaaatc gtccggttat
     8521 ccgtgatgat cgtccagatt tgctttaccc aacattacaa agcaagttcc acgctgtcgt
     8581 gcaagatatc aaagatcgct accgcaaagg tcaacctgtg ttagtcggaa ctgttgcggt
     8641 tgaaacatct gagttattat cagatatgct gaatcaagaa cgtattcctc atgaagtgct
     8701 gaatgcgaaa aatcacttta aagaagcaga gatcatcatg aatgctggtc aaaaaggtgc
     8761 tgtgacgatt gctaccaaca tggctggtcg gggaacagat atcaagttag gacttggggt
     8821 aagagaactt ggtggtctag cagttatcgg gacagaacga catgaatctc gtcgtatcga
     8881 taaccagctt cgaggacgtg caggacgtca aggagaccct ggtatgtcac agttctactt
     8941 gtcattggaa gatgatttga tgaaacgatt cggttcagaa cgaatcaaat ctttcttaga
     9001 tcggatgaag atcggtgagg aagatgctgt tatccaaagc aagatgctga cgaaacaagt
     9061 agaatctgca caaaaacgtg tagaaggaaa taactatgac acacgtaaaa acgtcttaca
     9121 atacgatgat gtgatgcgtg aacaacgtga agtaatctat aaacagcgac aagaagtaat
     9181 catggaagaa aaaagcttat ccaagccact aatgaacatg gtcaaacgga caatcagtcg
     9241 tgtagtagat gcgcatacac aattagagaa agaaaattgg aatttggaaa gtatcatcga
     9301 ttttgccggc agtactcttg tccatgaaga tagtattact cttgcagata tcgaaggaaa
     9361 aacagctcag gagatcaaag agtatctaaa cgaacgagca caagaagtat ttaataccaa
     9421 agcggctcaa ctaaatggtc cagagcaact gttggaattt gaaaaagtcg tgatcttgcg
     9481 tgttgtggac tctaaatgga cagaccatat tgatgcaatg gatcaattac gtcagtcaat
     9541 cggcttacgt gcttatggac agaacaatcc attagtcgaa tatcaaacag aaggctataa
     9601 gatgtttgaa gatatggtag gcgccatcga gtatgaagtc actcgaatct ttatgaaagc
     9661 tgagatccgt caaaacgtac agcgtgaaca ggtttctcaa ggaacagctt ctcactcaga
     9721 agacggagat gccaaagaaa atagtaatac tagcgcgaaa aaacagccaa tccatgtgaa
     9781 caaggtcgga agaaatgatc cttgtccttg tggaagcggg aaaaaatata agaactgtca
     9841 tggaaaaaat gcaaactaat ttctgacagc aggattggtg agacaaattc tttttttaag
     9901 aattcgtctc accttctttc tgttatttgc tgattctgtt agactagata taatgaaaaa
     9961 agtatagtga aagaaggaaa aaacatggaa atatccgaaa ttcgaaatct cctgacagaa
    10021 atgcaggaaa aggtcacaag cttcaggagg tctctttgac ttagaccagc tggaagaaga
    10081 cattgcggaa gcagaagcaa aaatggctga acctggtttt tgggatgata gcgaagctgc
    10141 tcaaaaagtg atcagtgaaa acaatgcaaa taaagaaact tatgatcaat tccattctct
    10201 tgctgaagaa ctggaagaaa tcgaattgat ggtggagatg ttgcaggaag aacctgatgc
    10261 agccatgcaa gaagaagcag aaacacgtat caatgcgcta gatgaaaaaa tgaagacata
    10321 tgaattatct atgttgttag atggtcctta tgatcgaaac aacgccatcg ttgaattgca
    10381 tccaggggct ggcggaacgg agtcccaaga ttggggcagt atgcttttac gtatgtatac
    10441 acgctgggca gagcagcatg ggtatcaagt agagacacta gattatcaag caggtgatga
    10501 agcaggaatc aagagtgtga ccctactgat caaaggacac aacgcatttg gttatctgaa
    10561 atcagaaaaa ggtgttcatc gccttgtacg tatctcgcct tttgactcag cgaaacgacg
    10621 tcatacttct ttttgttcgg tagatgtgat gccagagctt gatgaaacga tcgatataga
    10681 aatcaatcca gatgatttga aaatcgatac gtaccgagca agtggtgcag gtggtcagca
    10741 catcaataaa acagaatctg ctgtacggat cactcacttg cctacaggaa cagtagtagc
    10801 tagtcaggca caacgttcac aattgaaaaa ccgtgagcaa gcgatgggga tgttaaaagc
    10861 taagctttat caattagaag tggaaaagaa agaacaagaa gcagctgcac ttcgaggaga
    10921 acaaaaagaa atcggctggg gttcacagat ccgttcttat gttttccatc catattctat
    10981 ggtaaaagac catcggacaa acgtagagac aggaaatgtc caagcagtta tggacggtga
    11041 ccttgattta tttattgacg gctatttaaa aatgcatcta caataagaac ccattgttaa
    11101 attgaaataa aattgtaaga ttttgcaagc atgttgaaac attgccatgc tataatagct
    11161 aaagactgaa aaagatatgg agagaacgcc atgattgaaa tgaaggatgt aatgaagaag
    11221 tactcaaatg gtacgaccgc tatccgcaat ctctcaattg tcatcgaaca aggagagttt
    11281 gtctatgtgg ttggtccgtc tggagccggt aaatctacat tcatcaaatt gatgtatcga
    11341 gaagaaaaag cttcaaaagg aactttgaac gtcgcaggtc atgatctaat agcaatcaaa
    11401 aaccgcgaag ttccttatct acgcagagaa atcggcgtgg tgttccaaga ctataaatta
    11461 ttaccaagaa aaacagtata tgaaaatgtt gcgtacgcta tgcaagttat cggcagaaaa
    11521 cctcgggata tcaaaaaacg agtaatggaa gtgctggacc ttgttggttt gaaacacaaa
    11581 gttcgagtct ttcctagcga attatccggt ggggaacagc aacgtgtctc tattgctcgg
    11641 gcaatagtga atacgcctaa agtacttatc gcggatgaac cgacaggaaa tctggatcca
    11701 gaaaactcat gggaaatcat gaaattatta gatcggatca atgcccaagg aactactgtc
    11761 gtgatggcta cacataacag tacgatcgta aatacgattc ggcaccgagt aattgctatc
    11821 gaaaacggcc gcattattcg agatcaagcg gaaggggaat atggatacga tgattagaac
    11881 ctttttccgc catttattag aaagtatcaa gagtttgaaa cgtaacggct ggatgacagt
    11941 tgcttcagtc agtgcggtaa cgatcacatt gactttagtc ggtgtattca tggcagttat
    12001 catgaacgca actaaattag ctcaagatat agaaggaaac gttgatgtct ctgtattcgt
    12061 agatatcggt acaaaagacg aagagatgaa aaagctagaa aaagaattga atgacttaga
    12121 tcatgttaaa aaagttgaat tttcaagcaa agaacaggaa ttgaaaaaaa tccaaaacga
    12181 aatgggtgat tcctggaaat tgtttgaagg tgacaataac ccgttatatg atgtgtatgt
    12241 agtaagtgct actgatcctt cttatacaaa acaagtagca gaagaagcag cagatttgaa
    12301 gaatgtttct cgttctgatt acggtggagc ttcctcagat cggattttcc aaatcgctgg
    12361 tgcagttcgg acatggggat tagttgcagc ggcattgctg ctgttcgtag cgatgtttct
    12421 catttcgaac acgattcgga tcacgatttt gtctagacag cgtgaaattc aaatcatgcg
    12481 tttagtcggt gcaaaaaatg gatatattcg ctggccgttt ttccttgaag gtggctggat
    12541 cggtctgatc ggtgcgattc ttccgatttt gatcatcaca tttggttatg catggttttt
    12601 ccaattgatc aatccatcat tattacgttc ccattattca ttgattcatc cacaaaatat
    12661 cgtttggaaa atcaatcttt taatggtcgg cattggtgtg atcattggtt cacttggctc
    12721 gatcatttct atgagacgtt tcttgaaatt ctaataccac aatataaata aaatgagaaa
    12781 gttggacagt ttcgttccag ctttcttttt ttattggcta ggagaataga aggaagcggc
    12841 ttttttccct ttactttaaa aaacaacttg ggtattctta agttggtaga agaagaggtg
    12901 aagccgtgaa aattatcgtt gacaattttt ttacgatcct tttgattatg aatattttgc
    12961 tttcctttat catcgttttt cgagaaagaa aggaaactgc tcaaacatgg gcatgggtgc
    13021 tcgttttgat gttcattcca gttgtaggat ttattttata tattttttta ggaagaggga
    13081 tttccaaaga gaaaatcttt gatttgaaga tccaagggaa aatcggtaag aacctggaaa
    13141 ttgaagaaga caaacaggca ttgatgcgag ggctttatcc tcatccgcct actggacaag
    13201 tagatgtgaa acaattgatc tatatgctca cggtatttga aagtacgctc tatacgacta
    13261 aaaatgaaat cactttattt acagatggtc gtgaaaaatt cgatgcgctg attgaagata
    13321 tcaagcaggc agaagaccat atccacttcc agtattatat ttatcgcagt gatgcattag
    13381 gtgaagaagt tcgagatgca ttgacagagg cggcaagacg aggggtaaaa gtcagagtcc
    13441 ttcttgatgc atggggttcc acacaagttt cttccagctt ttttcagaat ttgaaaaaag
    13501 caggcggaga agtcgcattt ttctttcctc tatttgttcc gtatattaat cctcggatca
    13561 attatcgcaa tcatcgtaaa atcgtagtta ttgatgggaa aatcggatat acaggtgggt
    13621 tcaatgttgg aaatgaatat ctaggattgg taaagaaatt tggttattgg cgagacaatc
    13681 atttgcgtat ttacggagaa gcggtgtata ccttgcaaaa ccgttttttg atggactgga
    13741 attctcagca tacaaaagaa gtaggctatt ctcccaaatt ttttccgtcc attcactcta
    13801 ctggtgatat agctgttcaa atcgtgacaa gcggaccaga taccgaacat gaacaaatca
    13861 aaatgactta tttgaaaatg atttcgttag ccaaacggga gatattgatc caaacgcctt
    13921 attacatacc ggatggatcg attcatgaag cactgaagtt ggctcttctt tcaggcgtaa
    13981 aagttcatat ccaaatacct aacaaaccag atcatcttct cgtttactgg gcaacgtatt
    14041 ctttcgctgc agagctaatc gagtatggag caaggatcga aacatatgag aatggattta
    14101 ttcatgcaaa gacgatgatt atcgatggtg gcattgtttc agttggctca gcaaatatcg
    14161 atgttcgttc atttcgcttg gactttgagg tcaatacact ggtttatgat gaacgaatgg
    14221 cttcacgtgt aagaaaagct ttttttgaag actcgaaaat ttctacgcat ctcacaaaag
    14281 aaatgtatga aaaccgagga ataatcatca agatgaaaga aggattagca agattgattt
    14341 cgccgctcct ttaaaacata aaaaggacat gagaaccatc aacagatgga gatctcgtgt
    14401 ccttttctaa cggggccatt tctgagagta cgtcagaaat ggagaaagcg tctcgcatga
    14461 gcgaaattaa ctttggggta tctcttgttt tttggggaaa gcaagaaata agctaatata
    14521 tgctattaaa agagacacat tt
//
LOCUS       DAXWOO010000063        14430 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.063, whole genome shotgun sequence.
ACCESSION   DAXWOO010000063 DAXWOO010000000
VERSION     DAXWOO010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.063"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(82..777)
                     /locus_tag="ACGE70_001480"
     CDS             complement(82..777)
                     /locus_tag="ACGE70_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFF8693122.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(867..1967)
                     /gene="ychF"
                     /locus_tag="ACGE70_001481"
     CDS             complement(867..1967)
                     /gene="ychF"
                     /locus_tag="ACGE70_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFF8693123.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(1976..2167)
                     /locus_tag="ACGE70_001482"
     CDS             complement(1976..2167)
                     /locus_tag="ACGE70_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFF8693124.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(2188..3078)
                     /locus_tag="ACGE70_001483"
     CDS             complement(2188..3078)
                     /locus_tag="ACGE70_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFF8693125.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(3065..3832)
                     /locus_tag="ACGE70_001484"
     CDS             complement(3065..3832)
                     /locus_tag="ACGE70_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFF8693126.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(3844..4557)
                     /gene="rsmG"
                     /locus_tag="ACGE70_001485"
     CDS             complement(3844..4557)
                     /gene="rsmG"
                     /locus_tag="ACGE70_001485"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFF8693127.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(4789..6690)
                     /gene="mnmG"
                     /locus_tag="ACGE70_001486"
     CDS             complement(4789..6690)
                     /gene="mnmG"
                     /locus_tag="ACGE70_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFF8693128.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(6707..8104)
                     /gene="mnmE"
                     /locus_tag="ACGE70_001487"
     CDS             complement(6707..8104)
                     /gene="mnmE"
                     /locus_tag="ACGE70_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFF8693129.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLNTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            8403..8786
                     /locus_tag="ACGE70_001488"
     CDS             8403..8786
                     /locus_tag="ACGE70_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693130.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(8954..9712)
                     /gene="jag"
                     /locus_tag="ACGE70_001489"
     CDS             complement(8954..9712)
                     /gene="jag"
                     /locus_tag="ACGE70_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFF8693131.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(9730..10548)
                     /locus_tag="ACGE70_001490"
     CDS             complement(9730..10548)
                     /locus_tag="ACGE70_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFF8693132.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(10545..10901)
                     /gene="rnpA"
                     /locus_tag="ACGE70_001491"
     CDS             complement(10545..10901)
                     /gene="rnpA"
                     /locus_tag="ACGE70_001491"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFF8693133.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(11019..11153)
                     /gene="rpmH"
                     /locus_tag="ACGE70_001492"
     CDS             complement(11019..11153)
                     /gene="rpmH"
                     /locus_tag="ACGE70_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFF8693134.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            11676..13010
                     /gene="dnaA"
                     /locus_tag="ACGE70_001493"
     CDS             11676..13010
                     /gene="dnaA"
                     /locus_tag="ACGE70_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFF8693135.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            13208..14338
                     /gene="dnaN"
                     /locus_tag="ACGE70_001494"
     CDS             13208..14338
                     /gene="dnaN"
                     /locus_tag="ACGE70_001494"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFF8693136.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
BASE COUNT         4006 a         3041 c         2582 g         4801 t
ORIGIN      
        1 tattatttaa ctatggataa acaaaatgag gttctttact acccctagat atctagcaaa
       61 atagctgatc tttttgctag tttatcttct tgggacggat aaacttccaa taatcccgta
      121 ttttttcttc aagtagtaac ggattgctaa agcagttgcc ccgataatga taactactac
      181 tggatctaat actggattga taactggtgg caatagtgtt gtcgcagaaa acagtacgac
      241 ccaaagaagt gtcgttccac ctaggataag caatgtctta cccattcctg gtttcttgct
      301 tcgatctgct ccgggatatt catattgata gatgtatttg tacatcatat aaaagaccca
      361 gccaccgatt gcagaaccta gaacgagtgt agaaaagcca taagctggcg ttgttccacg
      421 tgaaaacaat ccggtaatcg caatcatgat acttaataga ccaagcaaca gcaacgtatt
      481 atctaaccac atcaataaag gggtggatga attttttact tctgctggct tattgatgat
      541 tgcttccgtt ctttcagaga ctgtcccaaa caattgacgg gctgttcttc cgcctttttg
      601 ttctgcaact aaagtaggca gaatgtcatg aagggctttt actttttctt cttcagacaa
      661 attcgctgct tctaaagatt ttttcaaatc aaaaatgtac tgttgatttc ttttggttaa
      721 ttttgtttct aaactattgt tttcttttac gattgctcgt aattgttctg gttccattat
      781 ctaatagtcc tccttagccg ttccttttct caacggtcgt cacaaaagac attctaccat
      841 attctacagc tagtttgata gcccttttac acattaaagc ggaataacat tacgtcgccg
      901 tcttgtacga cataatcttt tccttctaat cgaacacgtc cagcttcttt tgctgcatgc
      961 atgtttccgt aatgatccaa gtcttcaaaa gagacagttt ctgcacggat aaatccgcgt
     1021 tcgaaatctg aatggatgat tcctgctgct tgtggtgctt tgattccttt tttgaatgtc
     1081 caagcacgta cttcttgttc cccagcagta aagtaagtag ctaagcctaa caaatcataa
     1141 gcagcacgga tcaactgatc caaacctgat tcttcgattc ctaaagcttc taaaaattcc
     1201 gctttgtctt cttcatctaa ttctgcgatt tcttcttctg cacgagcact gaccacgatc
     1261 acttctgcat tttcttctgc agcaaagttg cgcacttctt gtacataatg attcgcgtct
     1321 gcatctgcta cgtcatcttc tgagacattg gctacataaa gaattggttt tgtcgtcaat
     1381 aaaaatagtg atttaacgat tttttgttct tcttcagtaa attcgatcgt tcgggcagat
     1441 tttccttctt ccagtacagg tttgatttta tccagtacag ctagttctgc cactgcatct
     1501 ttatctttgg ttttagcaac ttttgctaca cgagtatagc gtttgttgat agattctaag
     1561 tctgctaaca ctagttccaa gttgatcgta tcaatatctt ctagtggatc aacacgtcct
     1621 tctacgtgag taatattatc atcatcaaaa cagcgaacaa cgtgacaaat agcgtctact
     1681 tggcggatat ggcttaagaa ttgatttccc aaaccttctc ctttacttgc gccttttacg
     1741 attccagcaa tatctgtaaa ttcaaaagtc gtcggaacag tttttttcgg atgtacaagt
     1801 tctgttaagc gttgcaaacg ccagtcaggt acttctacca tccctacgtt ggggtcgatc
     1861 gtcgcaaagg gatagtttgc tgcttctgct cctgcttttg tgatcgcatt aaacaaagtt
     1921 gattttccta cgttgggcaa cccaacaatt ccagcagtca atgccataaa tttattcact
     1981 ctttctctgc atttttttca attacttttt ttaatttctt ttcaaaatct cgacgggtca
     2041 tcatgacgat atgtccgcaa ttcatacatc tgattttgat atctgcgccc attcgaatga
     2101 cttcccagcg attcgtctgg caagcgtgag gttttttcat ttcaacgata tcgtgcaagt
     2161 cgtacataaa aatagcctcc tttttgttta ttcttcatca aattgaatat tcaagatatc
     2221 aagaattcga gtcaaatctt tttgagaaag atattcaatc tcgatcttac ctttcccatt
     2281 tttttcttgg atagctacac ttgtaccaaa tttatccatc aaacgatctt cgctttctcg
     2341 gatataatat ggtttttcct ttgctagcct tgggattttt ttcttttctt ttccatcatt
     2401 ttgatttaac tcagaaacaa gctgttccag ttggcggaca gttaaatttt cagttactgc
     2461 acgattggct aatttcaaga tcaatgtttt attttttaaa cctaacagcg tacgggcttg
     2521 tcccatagat aaacgctgat cttgtaccat ttccttgaca agatccggta aagaaagcaa
     2581 gcgcaaataa ttggcgatat aaggtctgct ctttcctaaa cgactagcta cttctgcttg
     2641 tgttagtttc aggtttttca tcagcatctc atatgcttct gcttcttcta acggattcag
     2701 atcttctcgc tgcaagtttt ccagcactgc tacttgcatc atcgcttctt cgtcaaagtc
     2761 tctaacgata gcagggatcg tttctttttc agctagtttg gacgctcgaa aacgtcgctc
     2821 ccctgcaata atctcatatc cttttactgc ggaacggcgt acgatgatgg gttgaaagac
     2881 acctgattgt tcaatagaat tagctagttc ctgcaaagat acttcttcaa atgttttacg
     2941 gggctgatac ggatttggtc gcagctcatt caatggtagc tggacaacgg tatcatcttt
     3001 tacatccacc gtctctaagt tttctaaatc ttgaaacagt gcatcgattc ctcggcctaa
     3061 tcctttactt ttcttcacga cttaccactt cctttgccaa tgcttggtag acttcagctc
     3121 ctcgagaacg tggatcgtag tcgatgatcg gttttccatg actaggtgct tctgataaac
     3181 gaatatttct tggaatgatc gtttcgtaaa ctttttcgcg gaagtattta cgtacttctt
     3241 ctacgacttc atttcccaga tttgttcgtg catcatacat tgtcagtaag actccctcga
     3301 tctctaattc tggattaaag tgtttttgga ctaaacgaac cgtattcagt aattggctta
     3361 atccttccaa tgcgtaatac tcacactgaa caggtatcaa aatcgaatca cttgctgtaa
     3421 atgaattgat tgtcagatgg ccaagagaag gcgggcaatc gatcaaaata taatcatact
     3481 gactgcttac ttctgctaaa gatcctttta atcttgattc tcttgccatc attgacgtca
     3541 attcgatctc tgctcctgct aattggagag tcgcaggcac aatactcaaa ttttcgtgtt
     3601 cagtgatcaa agttgcctca tcaattggca attcattgac tagaacatca tagatgtctc
     3661 ttgtcacatc aggttttctg atcccgacac cactggtagc atttccttgt gcatccatat
     3721 ccactagcaa tacttttttg ccaaggctag caagacaagc acctagattc accgtcgtcg
     3781 ttgttttgcc gacgccacct ttttggttcg caacagatat tatttgtgcc atctcttctc
     3841 ctcctatttg attggctgct tattaggcag tccgggcttt cttgggtatt tctttggggt
     3901 tggtttcttt ttttgaataa cgatgatatg ccgttgatca gagacattcg gcaaggtaac
     3961 cgcctcttct ttgaggaatt taccacctaa taaagcaatc gcttgttttg cttctgtcaa
     4021 ttcctcttca ctttttgctg ctttcaatgc ataaaaatag ccttcttgtt tagccaaggg
     4081 caaacacaat tccgctaaca cattcaaacg agcaaccgca cgagcagtga caaggtcaaa
     4141 tgatgcacga aagtgaggat tttgcccaaa tgtttctgcc cggtcatgat ataaggaagc
     4201 atcgattcct aataatcggg ttaactcaga taagaaggtt attcgtttat tcagagaatc
     4261 aacgatcgtt atctgcagtt ctggaaaggc aatttttaaa ggtatgctag gaaaccctgc
     4321 ccccgaacca acatcgcata aagataaagg ttgcgtaaag tctacttcaa atgctaatgt
     4381 aagtgaatcg taaaaatgct tcagatatac gtctttttca tccgtaatag ctgtaaggtt
     4441 catcttttcg ttccattcga ctaatgtccg ataataaata gcgaattgat tcatttgatg
     4501 atcggtaagc tctatccctt tttctgccaa ggattggcga aattcttctg gtatcataaa
     4561 tattcctttc attgtgaaac agcaattgtt tcacagattc tctccctact ttaacgcaaa
     4621 atctctagac atgcaatcct tccatatgga ttcttaaaaa tttgaaaagt aagaaaagcc
     4681 gatagacttt ttctttctag tatgactcat aaaatataca cagaagagag aactagcttt
     4741 gagcaatttc tgcaaaaact agttctctct tctatttatt attacgtatt acgctggttc
     4801 actgactttc gcaatcttcc cttgttcgat atagaccatt aggatactta ggtctgcagg
     4861 gtttacgcca ctgattcggc tagcttgggc aattgtttct ggttggattt tctgcaattt
     4921 ttgttttgct tctgttgcta atccgttgat tgctgcgtag tcgatgtttt ctggaatctt
     4981 tttcgcttcc atgcgtttta atttttctac tttttccatg gcttttttga tgtagccttc
     5041 atatttgatt tggatttcta cttgttcgat ttcttttcgt gtcaattctt cattttctgg
     5101 aataaaccgt agaagttctg cataagtaat ctcaggacgt cttaaaaagt cgcttgctag
     5161 tacaccatct tttaatggtg ccgcattaat cgttgctaaa aagtcttgga cttcttctgt
     5221 tggtttgatt cgtacattca tcaaacgttt gatttcttgt tcaactgctt cttttttctt
     5281 caaataattc ttgtattgat cttcttttac taagcctaca cggtgaccca tttctgttaa
     5341 tcgcaaatct gcattatcat gacgtaaaag cagacggtat tcagcacgtg aagtcagcaa
     5401 acgataaggt tcgttcgttc cttttgttac caaatcatcg atcatgacac cgatgtaacc
     5461 gtcacttcgt ttgatcacta aaggttcttt tccctgaact ttcaaagctg cattgatacc
     5521 ggcaatcagc ccttgtccag ccgcttcttc ataaccgctt gtaccatttg tttgtccagc
     5581 tgtataaaga ttttcaacga ctttagtttc taatgtcgga cggagctggt gtggctctac
     5641 tacgtcatat tcgatagcat atcctgtacg catcatttct actttttcta atccagcaat
     5701 agaatgaagc atgttgactt gaacatcctc tggtaaagaa gtagataaac cttgaacata
     5761 gacttcttcc gtatttaagc cttctggttc caaaaataat tgatgacgtg gtttgtctgc
     5821 aaaacgcacg atcttatctt cgatggatgg acaatagcga gcgcctaccc cttcaacgat
     5881 ccctgtaaac attggggcac gatgtaagtt gtctcgaata atttggtgag tcgtctcacc
     5941 tgtataggtc agccagcaag attcctgatc tagtttatag gcactatctg gtgtgctgaa
     6001 gctaaaatga ttcggctctt tgtcaccagg ctgctcttct gtaacagaat agtcgatcgt
     6061 acttgatttt actcgaggcg gtgttcctgt tttgaaccgt tcgatagcaa agccaagttc
     6121 ttttagatga ttcgctagtc caatagaagg ttgagagtta ttcggacctg atgaatattt
     6181 caattcaccg ataatgattt ctccgcgaag tgctgttccg gctgtaataa caactgcttt
     6241 ggcccggtag ctcgctcctg tggacgtcac tactccatga caaacaccat cttcgacgat
     6301 cagttcttcg acgattcctt gtcttagcgt caaatttggt tctttttcga tcgtatgttt
     6361 catttctgtt gaataagcat gtttgtctgc ttgtgctcgc aatgctcgga cagctgggcc
     6421 ttttcctgtg ttcaacatac gcatttggat ataggtttta tcgatattct tacccatttc
     6481 tccgcctaac gcatcgattt ctcgaacgac aacgccttta gctggtccgc ctacagaagg
     6541 attacatggc atgaatgcta ccatgtccaa atttattgtc agtaataatg tttttgatcc
     6601 catacgtgca gcagcaagtg cagcttctga accggcgtgt cctgcgccaa caacgatcac
     6661 gtcatacgca tctgcttcat aatgttccaa tcgtttgttc ctccttttat tttcctaaac
     6721 aaaattgact gaataattga gtgattagct catcttgtac actgtcgccg acaacttctc
     6781 ctagatagtc ccagcaacgc gtcatatcta tttgaacgag atccacaggc attcctgaat
     6841 caatacctgc aatgacttct tcaagtgaga gggaagcttt ttctaacaac gcgatatggc
     6901 gggtattaga aacataagta gcgtctcttt ctcctgtttc tccgctgaag aataaatcag
     6961 aaatagctga ttccaaacga tctagtccgt cgtttttagc tacggaaatt gaaaaaactg
     7021 gttcattttc gatcaatttc ttcaattttt cttgttccag ctgtgctggc aaatctgttt
     7081 tatttagaag aatgatccgt ttcaatccac ttgtcgcttc caataattgt tcatcttctg
     7141 ctgtcagtgg ttcgctttgg tttagaacaa gtaagatcaa gtctgcttct gctaatgctt
     7201 ttcggcttct ttcgaccccg atccgttcca ccacatcttc tgtttcccga attccagctg
     7261 tatcgattag cttgagcggt accccccgaa cattgacata ctcttcgatt acatctcgag
     7321 tcgttcctgc aatatccgta acgattgctt tttcttctct taacaaatgg tttaacaaac
     7381 ttgatttgcc gacatttggc cggccgatga ttgccgtatt taacccttcc cgcagaacct
     7441 tcccttgctt ggatgtagca agtagcgctt ggattctttg ttggatcatc tgcgcttttt
     7501 caagcaataa cttcgtagtc aattcttcga catcatcata ttctggataa tcaatattta
     7561 cttctacttg tgctaatgtc tctaaaatct cttgtctcaa tgagcggatc aaagcagaca
     7621 gattgccgtc taactgatta agagctagtc ccattgcttt gtctgttttt gcccgaatca
     7681 aatccatcac agcttctgcc tgtgaaaggt caacacgacc attcaagaat gctcgttttg
     7741 tgaattcgcc tggctcagct aatcttgcac cttctcttaa caatagctgc aaaatttgat
     7801 tgactacaac gatcccaccg tgacaattga tttctactac atcttcccgc gtaaatgtct
     7861 ttggtgctct catcacggag accatgactt catcgacaag ctgattgctc ttgggatcaa
     7921 cgatatgtcc ataatggatc gtatgacttg gtacttctga taatcgtttg tttccagatt
     7981 gataaacttt atctgctagt tgcacagctt gatcgccact taaacgaacg atactgattg
     8041 caccttctcc aggtggggta gaaattgccg caatcgtatc aaattctaaa gtaatttcag
     8101 ccattttttc acttcctttt tcattttgcg cacaaaaaaa gtgcccactc caccctttat
     8161 gcacaataaa cggtgaactt cgcactttga ctgcttgttc ttgattaaat tctggtcata
     8221 gattatcaca cttgctataa ggaatcaagt gtttaaaaca attagatgaa gttgagcctg
     8281 ttctgcttcc attaccttat atttaattgt ccttggatgt ctctttgcgt tctttggttt
     8341 attcgaagag aagatactgt ataaacaagg ctttatcttt cttaaaaaaa acggtacact
     8401 aaatggtagc gctatctaaa ctatacactt ttgaaaggag aaaatatgtg agcccgttca
     8461 gatctgctgc attggaagaa gaaagtgacc ctcggatcaa cgatgtcgtt ttatctacag
     8521 ggaatatcaa ccagaaatat atcgtccgcg atatcgtttt tgttgtggaa tgcttagaag
     8581 ctgatttgtt tgacccatct tttcccaaag atgacctttt cttaacaacg aagaggaaat
     8641 taaagacgaa ggtacttgaa tatggtgcga atgcagtaat caactgtcat tttgagtatc
     8701 agcaaaaaaa acgcgacgga aagtattttc tggaactttt tgcttatgga acagtgatcc
     8761 aatttatcca aacaacgatt ggttaaatga aacagacaaa aaaagcggat caaaactgaa
     8821 gacagtttcg atccgctttt ttctatcaaa gttagtgact acttcatcgt atactctgat
     8881 aagaatgatt tctagtaaaa tatacttggt ttgaaaaact ttgtcaacgt gatttattca
     8941 aaaagtaatg gaatcaataa tattttttag caggttctac tactaaataa cgataaggtt
     9001 cctcaccttc cgaatgtgtt tttacatgtt catctttgct taatgcagca tggatttgtt
     9061 tacgttcaaa tgcaggcatt ggttctaaga aaacaggctg ccctgtccgt tttacttttt
     9121 cagccgtgcg atcagctaaa cgctgaatga tctcttggcg cttttcacgg taattgccta
     9181 cattgactac tacagacagc ttatttcttg ctaaacgatg gatgaagact tgtgccagat
     9241 attgcaatgc attcagtgtt tttccatgtt tcccaatcag tatgccttgc ttttgtgttt
     9301 ctaaatggaa aacaaccata ttatttgatt ctctttccat tttgacaaga gctggagcat
     9361 caagctgttt tgaaatttca gtaagataaa ctgccaattc cgttaaagct gcttcatcac
     9421 ctagctcact tacttcttct gttttttctt caagaatcgt ttcgttgact acctcgatct
     9481 cagctgttgg ttcttcttcc aaaatttctt ctacagtctg ttctactgtt tcagaaactt
     9541 tttcgctgat acttggctgg atcgaaagcc gtgcattctt tcgacccatt cctaggaaac
     9601 ctttttttcc ttcttccagt atttcgattt cgacttggtc tttggccaaa cccaaatcag
     9661 ccagacctag ttggacagct tcgtctattg ttgcagcttc ataaatcggc attagcatta
     9721 cctcccctat tatttccgtt ttttcttagg attcattgct ttcttaagag cacgttctct
     9781 ttcgcggatt ctccgtgctt cttcttcacg ctcttttcga attttgaaag ggttgttgat
     9841 cagcagcgtt tgtacaactt ggaaggcatt ggaaactacc cagtataatg aaagaccgct
     9901 ggctaaattg acacccatca ctaaaatcat tacaggcatc acgaaattca tgatcttcat
     9961 tgatgcattc gattcaattt gactcatgct agataaatac gtacttgcaa aagtaaagat
    10021 tgctgctaaa atcggcaaaa taaatgttgg atccgcgttc cctaagttca accataagaa
    10081 atgcccttgt ttcaactctg gtacacgtga aatagattgc cataacgcca tcaagattgg
    10141 catttgtact aataaaggaa gacaaccagc atatggattg acgccatttt ctgcatataa
    10201 acgctgctgc tcttctctta acttactttg tgtttccgga tctcttgaac tatattgttg
    10261 ctgcagtgct tttaatttcg gttgtaattc ttgcgtttta cgcatgctct ttgtttggaa
    10321 atgcatcaac ggcaatagga tcacgcggat gatcagtgta aataaaatga tccctatccc
    10381 tgcattacca aatgacaacg ccttgatcgc ctcagcaaag taataaacaa tataacgatc
    10441 ccaaattcct gtactctgtg cgctgacttc cccagttccg caaccggata agacgatgac
    10501 caaactaacg ataccggcca tcaaaagtaa acgtttgtat ttcttcactt actcttttcc
    10561 tctctgtcga ttatttttgc aagttttaag acgtggacta aattggagcg aacctcatca
    10621 taagtcaacc ccttcacact tggtctagca atcacaataa aatccaactc cggatcaatt
    10681 tcttctttta tagaataaac tgctgctcgg atttttcgtt tgacttcatt gcggtttacc
    10741 gcgttgccga ccttttttcc tacagataaa ccaactcgaa aatgaggctg tcccggtttt
    10801 tccaaacggt aaacgacgaa tttgcggtta gcaaaagaat ctcccgtatg gaacacggct
    10861 tgaaactctc gttctttttt tactcgatac gcttttctca ttagttttct ttccttttct
    10921 atctctcact tacgctcata atcataccat aattacgatt gtcctttcat catttcccta
    10981 aaaaagagta taaaaaaaac cactgaaacc ccagtggcct aagctgaaag aacttttctt
    11041 cctttgcgac gacggctagc taacacacga cgtccatttt tagtactcat acgtttacgg
    11101 aatccgtgaa ctttttgacg tttacgttta tttggttggt atgttctttt catatattcc
    11161 acctccaaaa tgttcttatc tattttataa acacagacat actttgatag tataacgaca
    11221 aagtagtgat tttgtcaata taaaatagaa gatcgattgt taaaacttat ccacaagatt
    11281 atttttttct atgggaatcc tatgtggaaa aagttatgca cagggattat ccacagataa
    11341 taaaaggctg gttttacaca gaaaacacac gtgtaaaaaa agtaatccac aatcaggcga
    11401 tttcctgtgt attccgtctc ctaactattg gtatatctgt attttttatc cacagacgca
    11461 agaggaaatc tattggtttt tacgagtttt ccacctgtgc attagtggtg tggaaaactt
    11521 tttttgacaa ctgtgttttt ctcttttttc tttttgtcga ttgtgtggat aattgtaatt
    11581 aaaaaatttt tttcttcggt ttctgtggaa aactattgaa aaaaatgcta aattagtact
    11641 agttattcac tttttaatga aggaggaggc cttatatggt atccctcgat gctttatgga
    11701 atgaattaaa agcaacatac caaaaggatt tatcgcctgc tagctataat acatggatcg
    11761 aaacagcgaa ccctcgaacc cttgatcaaa accagctcgt tgttgaggtt cctagcaaga
    11821 ttcataaaga atattgggaa aaaaacctgg cgacaaaaat cgttgaagtc ggttatatgt
    11881 tatcaggaaa tgagattatc cctcgcttta ttactggcga agaagcagag caagaagaag
    11941 ttatagaaga aaaaaatcca aaagttgtgg cacctagtcc gctaaaaaaa gccatgctga
    12001 accctaaata tacctttgat acgtttgtca ttggtaaagg aaaccagatg gctcatgcag
    12061 cagcgctcgt tgtagcagaa gatccaggtt ctatttataa cccgctcttc ttttatggag
    12121 gcgtgggact tggtaagact cacttgatgc atgcaattgg tcatcaaatg ctgcaaagtc
    12181 agcctaatgc caaagtaaaa tatgtaagca gtgaaacttt tgctaatgat ttcatcaatt
    12241 cgatccaaaa caaaacagca gaagagttcc gtcaagaata tcgaaatgtt gatttactgc
    12301 tagtggatga cattcaattt tttgcagaaa aagaagctac gcaagaagag tttttccata
    12361 ctttcaatgc cttgtacaat gaagggaaac aaatcgtact aaccagtgat cggttgccaa
    12421 acgagattcc gaaactgcaa gaacgccttg tttctcgctt tgcatggggg ctgtctgttg
    12481 atattacacc gccggatctt gaaacaagaa cagcgatttt acgtaaaaaa gcggcggctg
    12541 aacgtctaga gatcccagat gacacactaa gctacatcgc aggacaaatt gattctaaca
    12601 ttcgtgaatt agaaggtgcg cttgttcgag tgcaagcttt tgctgcgatg aatagtgaag
    12661 atatttcgac tagtctagct gcagacgcac tgaaaacgtt aaaatcagga aaaggacatc
    12721 ctcaattgtc aatcctgcag atccaagaag aagtggcaaa gtattatcac atccaattga
    12781 aagatctcaa aggaaagaaa cgcgtcaaat ctattgttgt tcctagacaa atcgctatgt
    12841 atttggcacg tgagctcaca gataattctt taccgaaaat cggagctgaa ttcggtggca
    12901 aggatcatac aacggttatc catgctcatg aaaaaatcca gcagctgatg gattctagtg
    12961 tcagtatgca aaatgaagta tcagaaatca aaaacttttt actaaattaa tttgtcgatt
    13021 ctatcaactg aaaatagcat gtggataaaa gaaaaaaacg ttcaaaagtt atacacactt
    13081 tttccacagc tgttttttcg tttctctctt acgttttttg agttttccac agaatcaaca
    13141 tgccctatta cttttattat tctttttaac taataataaa taaacaaaca ttcaaaggag
    13201 atacatcatg aaagttactt taaaccgagc tagctttatg caggaattgc aaactgttca
    13261 acgagctatt tcaagcaaaa ccacgatccc tattttgaca ggtgtaaaaa tcacactgac
    13321 acaagaaggt ttgactttga cggggagcaa cgctgatata tcaattgaaa cttttttgtc
    13381 tgttgaaaac gaaaaagcaa atatgcaaat cgaatctact ggttccattg ttttacaagc
    13441 acgtttcttt agcgaaatca ttcggagact tcctgaagaa acatttactt tagaagtttt
    13501 agaaaataaa caagtagcga tcacttctgg aaaagcgaat tttatcgtaa atggattaga
    13561 tgcagataac tatcctcatc ttcctgttgt cgaaagccat aaccagatga aattacctgt
    13621 acacgtattg actaaactaa tcaacgaaac agtttttgct gtctcacaac atgagagtcg
    13681 tccaatcttg acaggtgtgc atttcatttt atctgataat tctttattag ctgtagctac
    13741 tgattctcac cgtctaagtc aacgcgtgat tccagtagaa caagcggctg atcattttga
    13801 tattgttatt cctggaaaaa gtttgatcga attatctcgc tcattaacaa atgaagaaga
    13861 aatcgtcgaa atcagcatta tggaaaacca agtgttgttc aaaacagaaa cgatgtattt
    13921 ctattcccgt ttgctagaag gaaactatcc agataccaat cgtttgattc cttcaagttt
    13981 taatacagag gttgaatttt ctgttcctag ctttttagcg gcgatcgaac gggcttcttt
    14041 actttctcat gaaggccgta acaacatcgt tcgtttatct attcgtccag atgcggttgt
    14101 tttatacgga aattcgccag aaatcgggaa agtagaagaa agcttgagtt atacagcaag
    14161 cagtggcgat ccattagata tttctttcaa tccggattat atgaaagcag cgttgcgtgc
    14221 atttggagat atgagcatca aagtgaaatt tatctctgct attcgtccat ttacattgga
    14281 accaacggaa gatggcgttc aatttattca gctgattact cctgtacgta caaattaaca
    14341 atgattttca atgttcttga aaatttaatt gaaaagaaat agaaaaaata aaattttaaa
    14401 cagaaggccc taaaatctct tttaaggctt
//
LOCUS       DAXWOO010000064        14082 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.064, whole genome shotgun sequence.
ACCESSION   DAXWOO010000064 DAXWOO010000000
VERSION     DAXWOO010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14082
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.064"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(402..1058)
                     /locus_tag="ACGE70_001495"
     CDS             complement(402..1058)
                     /locus_tag="ACGE70_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFF8693137.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            1251..1529
                     /locus_tag="ACGE70_001496"
     CDS             1251..1529
                     /locus_tag="ACGE70_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693138.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            1548..2756
                     /locus_tag="ACGE70_001497"
     CDS             1548..2756
                     /locus_tag="ACGE70_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFF8693139.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(2904..3269)
                     /locus_tag="ACGE70_001498"
     CDS             complement(2904..3269)
                     /locus_tag="ACGE70_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFF8693140.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(3416..4189)
                     /locus_tag="ACGE70_001499"
     CDS             complement(3416..4189)
                     /locus_tag="ACGE70_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFF8693141.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(4363..5223)
                     /locus_tag="ACGE70_001500"
     CDS             complement(4363..5223)
                     /locus_tag="ACGE70_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFF8693142.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(5223..6641)
                     /locus_tag="ACGE70_001501"
     CDS             complement(5223..6641)
                     /locus_tag="ACGE70_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFF8693143.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(6698..7147)
                     /locus_tag="ACGE70_001502"
     CDS             complement(6698..7147)
                     /locus_tag="ACGE70_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFF8693144.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(7592..7855)
                     /locus_tag="ACGE70_001503"
     CDS             complement(7592..7855)
                     /locus_tag="ACGE70_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFF8693145.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(7954..9249)
                     /gene="rho"
                     /locus_tag="ACGE70_001504"
     CDS             complement(7954..9249)
                     /gene="rho"
                     /locus_tag="ACGE70_001504"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFF8693146.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(9246..10517)
                     /locus_tag="ACGE70_001505"
     CDS             complement(9246..10517)
                     /locus_tag="ACGE70_001505"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFF8693147.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(10682..11551)
                     /locus_tag="ACGE70_001506"
     CDS             complement(10682..11551)
                     /locus_tag="ACGE70_001506"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFF8693148.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            11654..11866
                     /locus_tag="ACGE70_001507"
     CDS             11654..11866
                     /locus_tag="ACGE70_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693149.1"
                     /translation="MKMKIFKKFFAKNKISNSALIEKSLLLSIFFPTNNNKSEKEKSD
                     KKIAYPLLLLLFSFYQVQKLFCFVIC"
     gene            complement(11843..13453)
                     /locus_tag="ACGE70_001508"
     CDS             complement(11843..13453)
                     /locus_tag="ACGE70_001508"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFF8693150.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(13675..13920)
                     /locus_tag="ACGE70_001509"
     CDS             complement(13675..13920)
                     /locus_tag="ACGE70_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693151.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
BASE COUNT         4003 a         2974 c         2400 g         4705 t
ORIGIN      
        1 aaaatcgtgt cacagcccct tttgttgcaa ataaacggcg ttcaaaagct tacatctacg
       61 gtgataagtc cagccaagtt aaaaacataa gagactgttt ttacttggct cttcttatta
      121 cttgatgcag aacagctttt tcacaacctc ttggtaaacg gtgttctcct accaactcct
      181 cggtcacaag tcacaatatt catcaaacat gaaaagctgt tttttgaaat tgttccttgt
      241 gactcagagt taaacggcag gctcacaacc tcttggtaaa cggtgttcaa tcagctgacc
      301 ctcggtatta gctcaaaaaa cggcaaaata tgaggaacta tttttccgtt tgtttctcta
      361 atactcaggt caaaacgctg atttcacaac ctctttgttc atcaaaagat aatattgtct
      421 tctaacaagt ccatattcaa aataacgata tgatggtctt taacggtgat aataccatct
      481 tttttcagtt gttttaaaat tctgtttaca ctgctagcag ttgagatgcc acaaaatttg
      541 gcgatttctt cattcgtgat cgaaaaatcg atcagcatcc cttcttctgt ccgcacaccg
      601 aataaatcat ataattcgta aagttgcgta caaacagctc ccagctttcc gtttaatagc
      661 atttgctgca tttttttcat cgaataaagc agacgatagc ggtaatactc ttttacatag
      721 tgttgtagct ttgggtcttg gttgatattt tgccaaaagc gcacacgatt gattcgataa
      781 aactctgcat attctgattc aactcgaata ttatacggcg aatcgatgaa ttgtgaatat
      841 tcgtctttta aaagagagac gatttcaaga tctttgatat agcgaaggtt aaattctctc
      901 ccatcatttg aaataacact tgcttttacc gttccctctt tcaagatata cgtatgggaa
      961 tcttccatcc cttcgaatgt caaatattta tgatatcctt tgcgtataat cggaaaatca
     1021 tttttatcca aatattcctt taagactcta ctatccaatg atgctccccc atccaaactt
     1081 tcatctaaaa agatcataca gtactttaaa actttttaaa gtccaaaggt attttactaa
     1141 tgatattaac atttgttaag tacctttttc catcacttat gcgatttttt tccttttttt
     1201 tattcgttat actgcagaag taaaattttt taagaataga gggaaaaaac atgaattact
     1261 ggatgaacac aatcattaat cgattagaaa cagcatatca aacaagattt gatatgaaag
     1321 cttctttagt ctttttaaat gatgcgtatc aaaactcgat cgaattgatc aaagcagtgg
     1381 atgagaatcc aacaaacgaa tgcgaagaat ttttaaatct gtttatgtct acaagagatt
     1441 tatttattcg ccaacttgtt gatcgctatc catccaatta tcacgatgta gaagttcaaa
     1501 tacaaaaatt aaaagcatat tcagcttaag gggaatgtat aacatacatg gaaaagaaat
     1561 tatttaataa atattttatt ggaatcacat tgatcaattt tattgtctac ctgatttatt
     1621 atctgttgat ggtcatcatg gcagtcattg cgcaagacac cttacacgcc agcttgagcc
     1681 aagcaggttt tgcttctggg atctatatca tcggaacatt gctggcacgc ttattcatgg
     1741 ggaaacagtt agaactattt ggtcggaagt ttaccttaag aggcggcgcg atcttctatc
     1801 tcctttcaac attagcatat ctttatacgc caactatcgg ggcattgtat ttagttcgat
     1861 tcttgaatgg attcggctac ggtacagttt ctacagctac aaatgctatc gttactgcat
     1921 atatccctgc atctaaaaaa ggggaaggca tcaattatta tggattaagt actagtttag
     1981 ctgccgctgt tgggccattt ttaggaatga ttttgctgaa tatgactgat tttcgtttca
     2041 ttatctggtt ttcaattgta ctggtcttct ttgttacgat tgcctgcctt gtctttcctg
     2101 taaaaaatat tccattatct gaagaacaaa aaatatctct tcgctcatgg aatatcaata
     2161 gttttattga aaagaaagca cttttcatta caggaattgc ctttttgatg ggtctttctt
     2221 attcaagtgt tttatcattt ttatcttctt atacaaaagt aatccactta gttgctgctg
     2281 ggtcattttt cttcgtcgta tatgctttgg tcatcacatt tacacgtcca ttgactggaa
     2341 ggattttcga tgtcaaagga gaacaatacg tgatgtatcc tagctattta ttcttaacag
     2401 caggattatt tttactgagt gtaacgacga atagtgtgac attgcttatc tctggagcat
     2461 tagtcggcct aggttatggt acgtttatgt caaatggaca ggctgtttgt ttaaaaatcg
     2521 tccgcgaaca tcgaatcagt attgctttat ctacctactt tattggttta gacctaggct
     2581 taggcgttgg tccttatttg atggggatgc taaaatctct tacttcattt agaggattgt
     2641 atgtgattgc cggagtcatt ccattgatat gtacagcact ctatcttgtt aatggccgaa
     2701 aacatagtca tgaagcagag ggcgaattga ttgaatcatt gaaaaatgaa atttgatctt
     2761 gatgaataga taaaaaaaac cgttcagatc tgaaaataat ctgagcggtt ttttgagtaa
     2821 aaatagttta tgattattct taattaaaca tctatttatt tttaaagctt tcatttatcg
     2881 ataagaatag tttaactgtt gatttattcc attttccgtt catctggtac gatgccttga
     2941 cttagcaacc acttgtggaa gaaccattct gccacaccga tcaatagagc aggaatcaat
     3001 actttcatcc aaatatcgac tgtatcattc ataaaatata gatagagcca caagataact
     3061 gcagctgaac cagcatctgc gatcgtcgct gcgatgttcc ctactttacg taagatcaac
     3121 agatccccta gcacatagat catgactaca ataattaccc caacaatcgt tgaatccata
     3181 aagctaacac cgtataaacc tgtcagcacg acccacagaa tcgcccagat catgattgcc
     3241 ttcactacca acgccttcac atgtttcatt ttctgcactc ctttctcctg gataaaaaag
     3301 taagattatc taacaagata accttttcat tattatacca tcggctgaga gaagcgagca
     3361 aagataaggt cctatctaaa aatgaggaag ccacttagaa cattcacgta ataatttatc
     3421 cgtacagaca ttcgatatgg tgttccttta ctttcgcata ctgttctttt gtcagttctt
     3481 tacttgtcac cagataatcg atttcatgaa aagcaatggc ttgatgcatg aatttatgtg
     3541 caattttttg ctgtgtaact aaaacggcaa gccggtctga ttgttttcta aaagcttttt
     3601 tgaccattgc ctctccttca tgagtttctg ttactccttg gctgaaatct agcccgcgac
     3661 aactcattat cgctaaatcc gcataatatg acaatgcatc gttataggct tttgaaccgt
     3721 tgattgttgc tcgtttcgga acaattgccc ctcccaatac agatactttt gccgttgtat
     3781 attcactcaa catagcagct gtgacgatgc cattcgtaat gatctgcaaa ccatcaaatc
     3841 ttgctaggtt tctgactaag tgtaaagagg ttgtactgga atccaagaat aagaccatat
     3901 catctcgaat caaagaagaa gctgtatctg caatcttacg tttttctgtt tcttcttcta
     3961 atttggtgat cttcatcagg taaggcacgt caatttttcg attgtcaata agcatggcgc
     4021 caccgcgaac tctacggatc aatccacttt tttccagaaa aacaaggtca cgtttaatcg
     4081 tggcttcgct ataatttact aactcgatca aatcggtgac tcttgcaaat tggttgtttt
     4141 ttagataatt cacaatctcc tgctgtctat tctccacttc attcaacata aaatcacccc
     4201 atttacttta tatagtaaca ggaaaaaaga ggttgtgaca gaagtgattg gcttcgagaa
     4261 ataagaagaa atttttcgaa aaaactactt ctgaacatcg tgtgtggtta agagggtatg
     4321 acaagactat tgtcataccc tcttgaccac acatttttat ttttagtata aactagcctt
     4381 gcctactgtg ttcaaaattt ccattttatg tttgaccact tcttgtacct ttttattagc
     4441 tgctggataa attgcattcg gctcgtacat cgatggattt tcactcaata tttctctcaa
     4501 agcattgaaa aagacgcttt taagatctga gctcaaattc actttggcaa ctccgtattt
     4561 tactgcttcg ctgacttctg cgtccggatt acctgaaccg ccatgtaaaa caaatggaag
     4621 atcgatttgt tggttcaatt ctttcagtaa gtcgatattt aattttggag ttctgttttt
     4681 cggatacaag ccatgcgctg ttccaattgc tacagcaagt gtatcgattc ctgtttcttt
     4741 cacaaaaatc ttcgcttgat caggatctgt ataaatgatt gtttcagcac ctccttcagc
     4801 agaaccatta ttcccaatcg ttccaagctc agcctccact gagacattta ccgcatgagc
     4861 taacgcaacc acttttttcg taatctgtac attgtcgtcg aatgatttca aagacgcatc
     4921 gatcatcacg gaagaatagc cgtttcgaat cgcccgtaag acatcttcta tcgtaccgcc
     4981 atgatccaag tggataacga tcggcagttt acttgtataa gcataggctc ttactgccgc
     5041 aacaaactca tcacttaaat aggcgatttc atcaggatga atttctaaga ttactggtgc
     5101 ttctaatctc tctgcttctt cgatgattgc tttcatcata tcatagctgc aaatattgaa
     5161 ggctggtact gcaaattgat tctctttagc cactttcaat aactctttca ttgtatataa
     5221 cattacgctt ccacctcatt tgttagattt tcatttttcg tccacttgtc ttgttttttc
     5281 tctttcactg tttttttcaa tagtccaagc ataaaggctg tagtgactgt acctgatgca
     5341 attgccaaga catacatagg taaattgttc ataacatttg gaattagcat cacccaaatc
     5401 cctccatgag gtgccattga agtaactcct actgccatgg aaatccctgc agcgactgct
     5461 gaacctgtca tcaaactagg tatcacgcgg atcgggtcag ataccgcaaa cggtatagca
     5521 ccttctgtaa tatacgaagc gcctaaaatc cagcatgatt gacctgaagt ccgttcttct
     5581 ttggtaaatt tatgtttgaa taataaagtg gctaaagcaa tacctagggg aggtgtcatt
     5641 cctgcagcca tacaagcagc aatcggtgca tttactccag ctgacatcaa accgatcgag
     5701 aaagtcgaag tcgatttatt gatcggccca cccatatcag ctgccatcat gatcccgata
     5761 agtaatccga acaatagccc ggatgtattt cccaagctat tcagccaatt tgtcaaagca
     5821 tccaacaccc atgtgatcgg ttgttcaatc acaaatacca tcaaaagccc aataattaag
     5881 atcgataaaa aggggacgac aatcagctga taaatagcag aaaattcttt ttttacgcta
     5941 attttatttg ccagaaaatc acagatatac cctgctagca cgccaccaat cattccgcca
     6001 agaaaacctg aaccacctgt agaggccagt aatccggcaa caattcctgg ggcaaaacct
     6061 gcttttccag caatcgatgt agcaatcccc gcgcctaacg caggaaccat catggcaaac
     6121 gctgtatctc ctccgatcct tgaaaatgca gctgctaaca cattatagtc tgctgagttg
     6181 ggatcagccg ctgtaatccc aaaggcaaaa ctcagtgcga tgatgattcc gcctgcgatc
     6241 acaaatggaa gcatgtaatt cacaccattc atgaaatgat tatagattgt cggctttttc
     6301 tcatttgcag ctggttcttt ttcattagac acaacgaata cgccaatatc tctcaatgct
     6361 tcgtctatta ctacttgcgg ttctttgatt gctcggcttg tagagacacg ctttactttt
     6421 ttcccggcaa atcggtccaa ttcgatttct ttatctactg caagaatgat tgcatctgcc
     6481 tctttgattt cttgttcaga taactgattt tctgttttta caccttgtgt ttcgaatttt
     6541 gcttgatggt ttagatttct tgctgttact tctaattttt ctgccgacat ataagtatga
     6601 gcaattccag tcgggcaagc agttacacca acgattttca tgattatttt cctccttttt
     6661 cttcactatc gatactcgga caaagactct actattttca ttcttgacta atctgtctga
     6721 tgatatctgc tggcgtagtg gcttctttta ttccctttct aaaatctgca ttcatcaatc
     6781 gtctggagag tgtgctgaga atctctagat gcatatctgc tgatttttca ggaactacga
     6841 tggcaaaaat atattcaatt ggcatttcat ctatcccgct atagtccgtt aatttggctg
     6901 tatgaaaaaa acacagtgct ggttttgtca catatttgct ttttgcatgg ggaatcccaa
     6961 atccatcttc tattcctgtg gagatctcct cctctcgttt gaatagtgcc ttaacgactt
     7021 tttgaacatt tcttacctta tttgtttttt tcaacacatc ccctattcgc tcaaataatg
     7081 atccttttga atctatcgat ggatcgaaaa caatcagttc ttctgcgatc aattgatcaa
     7141 acctcataaa aaacctctcc cccacaaaaa atgttaacgt tttcttggaa ctgaggtcag
     7201 tatagcatcg ctagctgtac cgttcaatat tatgaatgaa cagaggaaaa agacccatca
     7261 tccgcgtgta taagtcatta aaatgaacga tctagctcac aaacaatcgc aagtctataa
     7321 caaaacaaaa agccttcctt gaaaggaaag cttagcttct aattttaggt aaatggtttt
     7381 ttacttcgaa ataacccctc ttcatttcgt taatataagg tgtttttatc aagtggataa
     7441 ttaaaactat atactagttt ttcattgttt tgtgcattat gatcctacga ctttttcagc
     7501 atcaaacgaa gttttaaatt ggcaaaaaac ataaaaaaac agactatccc tatcagatag
     7561 tctgctaaac gttgagttat cagcgttttt attattccgc agcgtttgcg tctttgagac
     7621 cgtatttttt gttgaaacgg tccacacgtc cgtctgcttg tgtgaatttt tgacgtccag
     7681 tgtagaatgg atgtgagtca gaagttactt cgacacggat gactgggtat gtattaccgt
     7741 cttcccattc aactgtttct tgtgaagttt ttgttgaacc tgacaagaat ttgaaaccag
     7801 ttgttgaatc catgaatact actgggtggt aatctggatg gatgttttct ttcatgattt
     7861 tttcgctcct ttgccctgct ccatttgctg aaccagagtt gatttgtcga tttacaacat
     7921 taacatctta acatgtttca gtcaaggttg caattatctt ttcagatttc tggtcggatt
     7981 ttttttacca aaggagacat cttgaaattc tttgaaaaat tgctgattgt tttttgtttt
     8041 acgcaaaaag cggatcagtt gttccgtata ttctaatgaa tctccagtca tattttttcg
     8101 aagtttccaa atttcttcta attcttcact tggcatcaat aattcttctt ttcgggtacc
     8161 tgattttttg atatcgatgg ctgggaagat ccgacgttca gataattcac gtgaaagttg
     8221 taattctaga tttccagttc ctttgaattc ttcatagatc acgtcatcca tacggcttcc
     8281 tgtatcgact aaagcggtcg ctaaaatcgt caaactgcca ccttcttcga tgtttcttgc
     8341 tgccccgaaa aatttcttag gtttatacaa tgcggctggg tcaatcccgc cactcaatgt
     8401 acgtccgctt ggtggaacga ctaagttata ggcccgagca agtctagtaa tactatccat
     8461 caagataacg acatctcgtt tatcttcgac taaacgcatt gcgcgttcca atactaattc
     8521 agaaactcga gtatggtttt gcggctgcag atcaaatgta gaagatacca cgtcgccttt
     8581 cacgcttcgt tctagatccg ttacttcttc cggtcgttca tcaatcagta gaacgattaa
     8641 ttctacttcc ggatagtttt cggtgatacc atttgcgatt tcttttaata cagaagtttt
     8701 ccctgctttt ggcggtgcga cgatcaatcc acgctgacca aaaccgattg gagaaaagat
     8761 atcgatcatt cgggtagata gtctttgcgg tgttgtttcc agttgaagct gtctttgcgg
     8821 ataaagaggt gttaaggctg gaaaatgcgg acgttctttt gcttcttccg gatcttttcc
     8881 attaacgctt tccacgtgca tcaaaccata ataacgttcc gattctttag gtggacgggc
     8941 ttttcctgcc actttgtccc catttctcaa actgaatcgt ctgatttgtg aggcagaaat
     9001 atagatatct tctgcacttg gtccataatt gattggccgt aaaaaaccgt aaccatcttg
     9061 accaacgata tccaatattc cttccatata ataaaatcct tgtttctctg cttgtgcacg
     9121 aatcacagct agagataatt cctttttatt catcttactg taataaggaa ttttaaattc
     9181 tttggcataa ctataaactt cttttaacgt tttgttctct agttctgcca ttgtcaaata
     9241 atcgctcatt cagctcccac ttcctctatc atttcaattg atgcccctaa ggctgttagt
     9301 ttctcgataa tatgatcata accgcgtaag atataatcta cattgtaaat cgtcgttgtc
     9361 ccttctgcca atagtccagc aatcactaaa caagcacctg cccgaagatc agaagccacg
     9421 acttctgtgc cgtgaagttt atttggacca ttcacgatga tcatatttcc ttccacggta
     9481 atatcagcgc ccattcgtgc tagttccggc acatggttca cacgtttcgc atagatcgta
     9541 tcgatgattt ctgaagtgcc tgtagtcatc aatagtaacg cagtaagtgg ctgctgcaga
     9601 tctgtggcaa atcctggata aggataggtc atgatgtttg cgggttttaa ttctttcgag
     9661 gggtaaactt cgatttcatc ttcgctgatt ttcatatgga caccaatttc ctgcaatttt
     9721 gctataaagc tttccaaatg ttcaaagatc acatttttca ctttgatacc atttcctact
     9781 gcagctgcta atgctagata ggttcctgct tcgatgcggt caggaatcat gaaatgccga
     9841 cagccatgta atgtttcgac tccttcgata cggatcacat ctgtacccgc tcctcgaact
     9901 tttgccccca tattattcag taaagttgcc acatctatga tttccggttc tcgtgcagcg
     9961 ttttcaatta ctgtctgtcc ttttgctttt acagcagcta acatcacatt gatcgttgct
    10021 ccgactgaaa ccacgtccat gaaaatccga ttgccacgca atcctttatt ttctgtccgt
    10081 aaatacatgg caccatgttc atttgtcact tcagcaccta gagcttcaaa tcctttgacg
    10141 tgtaggtcaa tcggtctagg gcctaaataa cagccacctg gcaggcctac taccgcttcg
    10201 ccaaatttac caagcagtgt tcccataaaa taataagaag cacgcaaact attgattttt
    10261 ccgtgcggca tcggaatcga tacgacttct cgaggatcga tttctagtat gttgttggaa
    10321 aaatggacag ttgcgcccat gatctctaaa atttcaatta atgaatgaac atcttgtatg
    10381 tccggcacac cttccaagat cactggggaa tcagctaaga ttgcagctgg aatcaatgcg
    10441 actgcactat tttttgcacc gctgatcgtt acttcaccag ctaaaggtcg gtttcctttg
    10501 attacgattt ttttcatctg tttccacctt gtttattaat cttttttcat ggatatgctt
    10561 gtattttatc atagataaaa aaaagaaacg agcatggtct gccgttttaa gtaaaaaaga
    10621 atggagagcg acttcaaaag tcgttctcca ttcctatata tcgtatctgt tatgtggatg
    10681 cttacgcttt gttagctgaa ccgaaccagt cgatatgaga tttagcatct tcaacgattg
    10741 ctgctttacc tggagctaat aatttacgag ggtcgaatcc tttaccttct ttatctttac
    10801 cagcttcgat atatttacga gtagctgcag caaatgataa ttggaattca gtgttaacat
    10861 ttacttttga gatacccatt gagatagctt tttcgatttg atcttgagga atacctgatc
    10921 cgccatgtaa tactaaaggc atgtctggac ctacagcatc agcaattgct tgtaagtggt
    10981 caaaagctaa tcctttccag ttttctggat attgaccatg gatgttaccg ataccgcaag
    11041 ctaagtagtc gatacctgta gcaaccattt gtttgcattc ttcaatatca gctaattcgc
    11101 cgttaccgat gattccgtct tcttctccgc cgattgaacc aacttcacat tcaactgaga
    11161 taccttttgc atgtgctttt tcaacaacgt ctttagctaa tttcaagttt tcttcaaatg
    11221 gtagatgtga accatcgaac attactgaag tatagcctaa ttcgatacat tccaaagcag
    11281 cttcgtaatc accatggtct aagtggatag caactggaac agtgatgttc attgcttcaa
    11341 ctaaatcagt gattaaatct ttacagattt tgtaaccacc catgtattta gctgcaccca
    11401 ttgatgtttg gataagtact ggtgcttgtt ttgcttcagc cgcttcaagg attgcttgag
    11461 tccattccaa gttgtttgtg ttgaatccgc cgacagcgta accgccttta cgagcagctt
    11521 ttaaaaattc agctcctgat actactggca taataaaatt cctcctaagt tttgtaaaac
    11581 taaatttgtt aagacaattc ttaaccaacg accctatttt accaaacatt tcggtagttt
    11641 tctactaaaa tcaatgaaaa tgaaaatttt caagaagttt tttgcaaaaa ataaaatatc
    11701 caatagcgct ttaatcgaaa aaagtctttt gctttctatc ttttttccta caaataataa
    11761 caaatcagaa aaagagaaaa gtgacaagaa aattgcttat ccccttctct tgctactttt
    11821 ctctttctat caagttcaaa aattattttg tttcgtgatc tgctaatgat gctccgacaa
    11881 atgctttgat taatttttgc gggcgatttg gacgagaaat caattctggg tggaattggc
    11941 atcccacaaa gaattttttc tctggcagct ctacgatttc tactaaacgg ttatctggtg
    12001 agacacctga aaagactagc ccttgttctt caaacaattg acgatatttg ttattgaact
    12061 catagcggtg acggtgacgt tcttgcacaa catcggctcc atcatatgct tcagccgtct
    12121 tcgttccttt tttgatttta catgggtata atccaaggcg taaagttcca cccaaattct
    12181 cgatgttttc ctgatcagcc attaaatcaa taatattgtt tgctgtatct ggattcgttt
    12241 cagctgatga cgcatcttct aatccaacga cgttacgtgc aaattcgaca caagccatct
    12301 gcataccaag acaaatacct aaaaacggaa cgtcattttc gcgagcataa cgaatcgctt
    12361 cgattttccc ttctactccg cgatcaccaa atccaccagg aaccaagatt ccatctgcgt
    12421 ctttcaaacg ttcttctaca ttttctctcg ttaattcttg tgctttgatc caatcgatct
    12481 cgatgtctga attataggcg aaaccagaat gtttcaatgc ttcaactact gagatatatg
    12541 catctggtaa ctcgacatat ttaccgacaa gcgcaatccg tacttttttc ttcaagttca
    12601 agacacgttc ttctaattct ttccattctg tcatgtctgc ttctggtgta tctagtttta
    12661 aatggtcgca aacaatttga tccattcctt gtgcttgtaa aactaatggg atggagtaaa
    12721 gtgtatctac atctggcgac tcgataactg cttctggttc gacatcacaa aattgtgcca
    12781 atttgttttt cacgctttga gaaacaggtt gctctgtacg taccaccaaa atattcggtt
    12841 ggatacctaa accacgtagc tcttttacac tgtgctgcgt tggtttagtt ttcatttctc
    12901 cagcagcttt caagtaagga atcaatgttg tatggatata cataacatta tctgtaccga
    12961 catctgcttt catttgacga agtgcttcaa ggaatggcaa agattctata tcccctactg
    13021 ttccccctac ttctgtaatg atcacatctg aatcagtcat tttagctgct cgcatgattt
    13081 tttctttgat ctcatttgta atatgaggaa taacttgaac agttgctccc aagtattcgc
    13141 ccttacgttc tttacgcaaa acttcagaat agatttttcc tgtcgtcacg ttggagtatt
    13201 tgttcaaatt gatgtcaata aaacgttcgt agtggcccag atccaaatct gtttcagcac
    13261 catcatctgt tacgaatact tctccgtgct gatatgggct catcgtcccc ggatccacgt
    13321 tgatgtatgg atcaaatttt tggatagtta cttttaagcc gcggtttttc aataagcgtc
    13381 ctaaagaagc tgcaacaatc ccttttccga ttgatgaaac gacaccgccg gtcacaaaaa
    13441 tatattttgt cataagttca aaaactcctt tttaatttat gtgcaggaaa agtctcagac
    13501 aataaacaaa gctccctatt cttcaataag aatagggagc cgaaatttct gtaaacttct
    13561 gaccctctaa aagggtgccc aaggtgtata atacaagctg actaagggaa agtcaagtac
    13621 tcaaatgaat actatccgct tacaattttt tctttttcga tatcctttga gaaattattt
    13681 cttaagtcct ttatgagtgc aaaaaaaatt taacccacat aataagcttg atataaacat
    13741 agctaacgtt aaacctaagc aaatcagaaa atcgttactg aaaaatttta agtgaaataa
    13801 tagataaaaa aataaaacaa acattatgat agataatatc ccacctaaca aagaactact
    13861 ttttcctact tgcagcaaca gtttatatag cttatcgtag aaacaatctt tttctttcac
    13921 ccttatttac ctcctttacg taaaaaacgt agtccacagt tctcggattt ttggcatatc
    13981 ttttctatta aataatatca aaaacaaaac aattccacta attttttcaa agatgacctt
    14041 tttttcaaca aaagtgcctt gtagggattc attgaaggta ac
//
LOCUS       DAXWOO010000065        14082 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.065, whole genome shotgun sequence.
ACCESSION   DAXWOO010000065 DAXWOO010000000
VERSION     DAXWOO010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14082
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.065"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..282
                     /locus_tag="ACGE70_001510"
     CDS             <1..282
                     /locus_tag="ACGE70_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693152.1"
                     /translation="SFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRI
                     PIYVIEDYIWKLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(363..932)
                     /gene="thiT"
                     /locus_tag="ACGE70_001511"
     CDS             complement(363..932)
                     /gene="thiT"
                     /locus_tag="ACGE70_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFF8693153.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNSWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(998..1100)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(1106..2077)
                     /locus_tag="ACGE70_001512"
     CDS             complement(1106..2077)
                     /locus_tag="ACGE70_001512"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFF8693154.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(2171..3544)
                     /gene="glmU"
                     /locus_tag="ACGE70_001513"
     CDS             complement(2171..3544)
                     /gene="glmU"
                     /locus_tag="ACGE70_001513"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFF8693155.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(4047..4865)
                     /gene="purR"
                     /locus_tag="ACGE70_001514"
     CDS             complement(4047..4865)
                     /gene="purR"
                     /locus_tag="ACGE70_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFF8693156.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(5032..5856)
                     /locus_tag="ACGE70_001515"
     CDS             complement(5032..5856)
                     /locus_tag="ACGE70_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFF8693157.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(5829..6509)
                     /locus_tag="ACGE70_001516"
     CDS             complement(5829..6509)
                     /locus_tag="ACGE70_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFF8693158.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(6532..7470)
                     /locus_tag="ACGE70_001517"
     CDS             complement(6532..7470)
                     /locus_tag="ACGE70_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFF8693159.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(7715..8566)
                     /gene="ispE"
                     /locus_tag="ACGE70_001518"
     CDS             complement(7715..8566)
                     /gene="ispE"
                     /locus_tag="ACGE70_001518"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFF8693160.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(8720..11293)
                     /gene="mprF"
                     /locus_tag="ACGE70_001519"
     CDS             complement(8720..11293)
                     /gene="mprF"
                     /locus_tag="ACGE70_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFF8693161.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            11434..12240
                     /gene="proC"
                     /locus_tag="ACGE70_001520"
     CDS             11434..12240
                     /gene="proC"
                     /locus_tag="ACGE70_001520"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFF8693162.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(12319..13875)
                     /gene="rny"
                     /locus_tag="ACGE70_001521"
     CDS             complement(12319..13875)
                     /gene="rny"
                     /locus_tag="ACGE70_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFF8693163.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
BASE COUNT         4081 a         3095 c         2441 g         4465 t
ORIGIN      
        1 tcttttttca aaaaagaaac acctgttgaa aaaatcagtg tggaattatt gaaagaaatg
       61 cgtaatttga tgcaggaagg gatcattttt tcttttgatc aagttggttt gatcgatggt
      121 aaatatcgta tcccaatcta tgtgattgaa gattatattt ggaaattaaa aggcacccgg
      181 tatcgagaag aggaaatcat cgctacttta atctactctg tcaccgatcg acactggcaa
      241 ttggtagaag atgccgatct ttcttatctg ccattgaagt agatctattt cttatcaaaa
      301 aataaaaagg aaacaagaaa ctaggacatg attctgtctc aagtttcttg cttctttttt
      361 atttacagct cctgtcgttt atctggcaca aaaatcgaag gagtcgtttg ttggatccct
      421 aataaaacga tgatcgttac aatagctgta ctcaacccgc tggcaccatt cattacgaaa
      481 gaaaagagcc aagaattcat cccccatagt gcatacgctc cccagaaaac cactcctgca
      541 ataaaatgcc aaaaatatct ggaaagtgcg ccaatcaatg caccaaacac gatgcttttt
      601 ctaatttgtt tcgtattatc gtctacatag cctttttgga catttgaagc catgagccct
      661 gctgcgcctg caaaagtata ggcaatcggg tactcaatca acacttgaac gacacttaaa
      721 taataaactt gtcccaacgg aaaatgtagg atgccccaaa tgaaacctgc cagcatccct
      781 gcctttgtcc ctcttcttaa cgcaaaaaga gtcaatggaa tctgccctaa tgaaatagaa
      841 aaactgctgc cgatctggat cggaatcaac gacaacacca tcgctaacgc tgcaacaatc
      901 gttccttcta cccaaactct attttttccc ataaaaaaac ttcctccttt gaataaaaaa
      961 gtcacaaagg aggaagcagc tttcgaaaat agtcaaacag ctgtttattc acaattccta
     1021 cgctcgcata accgatacag gtacgaaggg tttagaaata gattcaatct cagcccaatt
     1081 ggactcccct ttgtgagttt atttattatt tattttttgt ttcaaacaat ggactaactg
     1141 gtttgttttc atggatacgt ttgattgcat ctcccattag tccgccaaca cttacttcat
     1201 cgattttgtc gattttacga tcttctggaa gatagatcga gtctgtaaca actagacgct
     1261 cgatagctga atcttctatt ctttgtaaag cagggccgga taatactgga tgtgtacatg
     1321 aagcatacac actagttgca ccagcttctt tcaaagcatt ggcagccaat gtgatcgtac
     1381 ctgctgtatc gatcatatca tcgatcagta cacaagtttt gcctttgact tgtccaatga
     1441 tattcattac ttctgcaaca tttgcttttg gacggcgttt gtctatgatc gcaatcggag
     1501 atttcaaaaa ttccgctaat ttacgtgccc gtgtgacacc accatggtct ggtgaaacaa
     1561 cgacaacatc atcgcctttg attccttttt ctaagaaata attggcaata agtggcgcac
     1621 ccatcaaatg atcgactgga atatcaaaga aaccttggat ttggactgca tgaaggtcca
     1681 aagtcagcat tcttgttgca ccagcttttt gaatcatgtt tgctactaat ttcgctgtga
     1741 ttggttcccg tgcacgtgct ttacggtctt gacgcgcata gccataataa ggcatgacta
     1801 cgttgattgt ttttgcactt gctcgtttca gtgcgtcaat catgatcagc aactccatca
     1861 agttatcatt gactgggctg ctcgttgatt gaataacata gacatgcgat ccacgaatac
     1921 tttcttcaat gtttacttga atttctccat cactaaattg agtaacagat gattttccta
     1981 actcgacgcc gactgctgct gcaattttct ctgctaatgg gcgattggaa tttaacgcaa
     2041 aaatttttaa tcttgggtca aagtaatgtt ttgacatggg gacctccact ttctttttca
     2101 caactctaga attctaccta ataaatagta gtcatatttt ttaaataaat caagtgattt
     2161 tgcaaaagtc ttagtttaga taagggagtt tttttgcata tccttcttta ttgacttgtc
     2221 ttgctctcgc aattgccagc gcatattctg gaatattgtc tgtaattgtt gacccggcag
     2281 caatcgaact gtttttagct acctcgacag gtccgatgat attcgtgcta gaaccaataa
     2341 agctgtggtc gccgacagtc gtatgatgtt tgttctttcc atcatagttg acgaagacaa
     2401 caccacatcc gacattgatt tcttctccca gcgtagcatc accaacataa gtcaagtgac
     2461 cgactttagt tcctttgccg atttgagcat ttttcacttc cacaaagttt ccgatatgga
     2521 cgttttcgcc aatttcagct tttggtcgta aatgtgcata aggtcccaca tctgcatgtt
     2581 tcttgacatg gctgctttca atgactgagt tttcaatgac gacgtgatcc tcaatcctac
     2641 tgtctacgat ttttgaatgt gacccgatga cacaatcaga accaatgact gtatttcctt
     2701 ggatttgtac acctgcttcg atcaatgtgt ctggaccgat ttctacgcct gcatcgatgt
     2761 atgtcgttgc tgaatcgaca aagcttacgc cattgaccat atgcatctgg ttaattcgct
     2821 ttctcatgat ctcatttgct ttagctaacg cgatccgatc attgactccc attgattctt
     2881 caaaatcatc cgtttgataa gcagcaactg tatggccttc ttcttttaag atttcgatga
     2941 tatctgttag atagtattca ccttgagcat tgtttgttcc aactttgctt aacgcatcga
     3001 acaaagcttc gttgtcaaaa cagtaagttc ctgtattgat ttcttgtaca agagcttctt
     3061 ctggtgttgc atctttttgt tccacgattt tttctacgat cccaatatgg tcacggatga
     3121 ttcgtccata tcctgttggg ttttctgctt gtgccgtcaa gatcgttgca cttgcatttt
     3181 ttccttggtg atattcaaaa agattgttca gcgtttcagt agtcaataat ggtgtatcgc
     3241 cactgataac taacgttgtt cctttttttc cttccaagaa agaagcagct tgcaaaacag
     3301 catgtcctgt tcccaattgt tctgcttgta acgcatattt actgcgttct cctagttgtg
     3361 ctttaacttg ttctgctccg tgtccaacga tcgtaatcac ttcatctggt ttcgtttcac
     3421 tgacccgatt aatgatatgt tctaccatag gtttaccaga aacgggatgt aaaactttat
     3481 ataattttga cttcatgcgg gtacctttcc ctgccgccaa aataattgca taacgtgcgt
     3541 ccaactgcgt cactcctaaa tatagtttgc tgatttcagt ttatcccagt atacccattt
     3601 tttcaacaaa acaagtgatt ttcttttatc ccagcgtttt ctggataaaa aaatagatag
     3661 accataacgg gcctatctat ttggtaaccg gtgctctcct gccaactcct cggtcacaag
     3721 tcacactact cataaaacat gaaaagctgt tttttgaaat cgtttcttgt gacttagagt
     3781 taaacggtag gcttacaacc tcttcattaa acgtttttca atcagctgac cttcggtatt
     3841 agctcaataa acggcaaaat atgaggagca atttttccgt ttattgagct aatactcagg
     3901 acaggacgct gatttcacaa cctctagata aacggtgttc aaaagccagt ttctcggtat
     3961 taagccaaat ttatgaaaaa atgtggaaag ccattttcac aaaatttttc ttactactcg
     4021 aaactaagcg gctttttcac aacctcttat tctgaaaaat aatttcctgg cacaaccgta
     4081 atatttttcg tttgagtatc tacatctttg acataaagca gtgacgtata atcatcgatg
     4141 gcgcgatgac cgttgaattt tgattcggca aatacagtga tgccaacaag ttctgattcg
     4201 aattcttcaa tcaagctctt cattccgttg actgttccgc cgcctttcat gaaatcatcc
     4261 actacaagga cacgtgaacc acgtttcaaa cttctcttag acagttccat cttttcaatt
     4321 ctttcagaag atccagacac atagttgaca cttactgttg atccttctgt tatttttgag
     4381 tcccggcgta caataacgaa tggcgcattc agataatagg acacagcttg ggcaataggt
     4441 acgccttttg tggctacagt cattacagca tcgatttttt caccaaggta tttagaagca
     4501 atgattcttc caacttgacg cagcagttca ggttctccta acaaatcaga gagataaaca
     4561 tatcctcctg gaagcagacg gtcttgttct gatagacgtt ctgctagaga ttcaatatat
     4621 tttttcgcct cttcatatga aatttcaggg atgaacaata caccacctgc agcacccggg
     4681 attgtttcca aggttccata ccctcgttct ttaaatgttt tcttgatgat tcccaaatct
     4741 tcactgatag aagatttagc agattcataa cgttcggcaa agcttgttaa ggaaatcaat
     4801 tcatgcgggt gattcaataa atactgtgtc atgtcaacta gacgttcact gcgacgtact
     4861 ttcacctatt ccaactcctt ttaagaaatt ttatcgtttt acattatata agtttttctt
     4921 caaaaattcg agcattttat gaaaaaataa gaaaatagtt cggtatttat taaaataaaa
     4981 agaagcctat aaaaaatttt tcgtttttat aaggcttctt ttctttataa tttagttatt
     5041 ttttcttctg aaggcaacca atattcgttt gatgatattt acaatcagga acaaagcaat
     5101 aaagactaat gtgattgttg ctcctggagg tgtatctaga taaaacgagc tagttagtcc
     5161 tgacagcatc cctagaaatc ctacacaaac actgatagca atcacgacat tgaagctttt
     5221 gcctaatcgc ataccgattg ctgcgggaag aaccatgatt gcggatatca aaagtgcacc
     5281 ggcaatcggg atcatcacag caatagccac accagtaatc acattgaaca tcatcgacat
     5341 caaatgaacg ggtagcccat ccacgtgggc ggtatcttca tcaaaagtca gaacatacat
     5401 cggccgtttg aataaaaaga acaatatacc gatggcaacg aataagattc ctaaaaagat
     5461 gacttgctcc catgtaatgg tcacgatcga accaaacaga taggattgga tacttgttgc
     5521 tgaattccct ccgctcaaat tcatcaatac taacgctagc gccaaaccac cagccattaa
     5581 aatggcaatg gagatttctg aataggttcg gtagatcgtt cgcagatact ccaatatcac
     5641 agctgctaat acaacgacta gcatggtcgt aagtgtagga ttaaggttca aaaagaatcc
     5701 taacgctacc cctgctaaag agacatgaga aagcgtatca gccatcagcg attgtcttct
     5761 gatcactaaa aacacaccta gcatcggggc gattccggca atgaagatag cggcaagaaa
     5821 cgctcttctc atgaattcat atgaaagcaa cgccattgag aatcctcctt tctcaccagt
     5881 cggatctgtc tgtccgcata ctgtttgata tcttcatgat catgggtgat cattagaact
     5941 gatttcccat ggacatgcgc actatgtcgg agaagacgat aaaattcatt tctggattct
     6001 tcatccatac ctgttgttgg ttcatcaaga acaaataagt cagggtcagt agcaaaaata
     6061 cgcgctaaac tgattcgctg cttttgtccg ccagacaatt cacctattcg ccgatttcgc
     6121 atatcccaca tccctacagc ttctaaagca cgctgtacat gttgatgatc tttttcagag
     6181 agacgcttga accagcggcc tcttggatat cttcctgaac ggactaattc gatcaccgta
     6241 ctaggaaaac ctgcattgaa agatgcaact tgttgaggaa tataaccgat acttaatttt
     6301 tttccttctt gatttttctc agcaatcttt atcattcctt ttgaaggttt cagtaatcct
     6361 aacgatgctt tcaccaaggt tgacttggct gctccatttt ctccagttaa aatgacaaat
     6421 tcacccgcat ctacatgata agagacatct tctagtacag gttcatcatc atagtaaaag
     6481 gtcaaatcag atatttcgat ataacgcacc ttacaacctc cattctaaaa cttaattgat
     6541 actttctttt aaagctttca gatttttttc catgacagaa atataatttt cgcctgcttt
     6601 gatttgttca ttagtcaagc tttctaaagg attcaacact tctagcttca ctcctgtttc
     6661 attcgacaat gtctcagcta ccttcgaaga ggcattttct tcaaaataaa tcactttgat
     6721 gttgtttttc ttcacaaatt cttttagctc agctaagcga cttggtgtgg gttcctgatc
     6781 tggtgaaacg cccgaaatcg ctacttgttt taagtcgtat tgattcgcta aataagcaaa
     6841 agcagcatgc tgagtgacaa atgtgcggtt tgttgcatct tttagctcat cggagtattt
     6901 ttgatccagt gcttcaagct ttttgatata agcatcggca ttcttcgtaa aagtttcctt
     6961 tttatctgga tatttttcgc ctaattcttc agcaatcgtt ctgacttctt ttatagctaa
     7021 taccggatcg agccaaacat gtggatcgta ttcatgtgaa tgaccatgcc cttcttctga
     7081 ttcttcatgt tcgtctgctt cttgcgattc taaacggtcg attcctttag ctgcttcgat
     7141 catcaaggtc tgtttgctat ccactgaatc ctttaacgaa tccacaaaaa attccatgtc
     7201 tgaactatta tacacgaaaa catctgcatc ggaaatttct gctctttctt ttgcacttgg
     7261 ttcgaaatca tgcggctccg ttccagcagg gatcaaaagt ttcacgtttc cttcatcccc
     7321 tacgatctct tttgtaaaat catacatcgg ataaaaagtc gtaacgatcg tcaaatcctc
     7381 ttttttgtct gcttccttgt tcgtatttcc acatgctgca aacagtaaag cacctatcat
     7441 taccgtagct gtcaaaagat attttttcat ttatatgctc ctttcaatga acaaatcgga
     7501 attttacgat ttattaacgt gaactaatgt atcaaaataa aaaaacacag tcaatagaaa
     7561 tccatcaatt tctgatttaa gcatgtatat tcttgattaa atcgtaaaat ggatgagttg
     7621 ctgctcatat ctagccattt cactacaata aaaaactgaa gaataccatt ttaggattcc
     7681 tcagtttttc atcacctatt ttacttctta tattttattg cagggttcgt actaaataaa
     7741 cttcctcaca aaaacctttt aacccattat atactctctg cgctcttgaa taatgttgac
     7801 acaaggcaaa aacggttggt ccgcttccac tcatcaatgc agcatctgct ccgtacttga
     7861 tcatacgctc tttgatttga tggatgactg gatgccgctt tcccgttata tcttctaaac
     7921 tgtttcccat ttttttgatc atctgctgat aatcttgctc tcggatggct tcagcaagtc
     7981 cgtggatgtc ttgatgttgg atggcttgcg tatctacttc tctaaacact gtacgtgtag
     8041 agacactcat tcttggtttg accaacacaa cccagcactg tggcatagaa ggtaatactt
     8101 ggatttcttc ccctttgcca ccgacaaatg ccgtacttcc gtagacacag taaggaacat
     8161 ctgtaccgac ttccaaaccg atttctgcca gttcttctaa cgaacagccc agattccata
     8221 attggttgat ccctcttaga gcagctgcac agtctgtact tcctccgcct aatccagctg
     8281 ctactggaat attcttctta acaaagatct tgacaccggt tttgatattg tatttatttt
     8341 ttagcaataa agcagcttga taaacattgt tccgttcatc taccggtaaa aatgctttat
     8401 ttgtttctac aatgattttg tcttctgtta tttcttcaaa aaatagatga tctgccaagt
     8461 ctacgctcga catcaccatt tccaactcat gatacccatc ttgccgtcta tataatacat
     8521 ccagtcctaa atttatcttg gctggcgctt tttcaatcaa ttccatcctc tcacccattc
     8581 gaaaagtcta agtcgcaagt attctatcat agggaaaaat aaaagtatag cttatgtctg
     8641 cctagtggtt gattttgtca tcgtttcaac agctttcaca gaattttctc ttcataatcc
     8701 aaacaagttc tagtgattct tataattctg tgagcacttc ttctgccaaa ctcggaacat
     8761 ttcctttttc ttttcctatc agactgagta gctgcatggt agcaaatccg aaattacttc
     8821 ctttcctata agcaaaataa cgtccttccc aatgatcgac atattttaat ttcttttcat
     8881 aagcagactg gaaggaatag aaaaaattac cataattata tacgatatgc atgacttttt
     8941 cttgaaagaa agaaaaatct gtttctccaa cattagctaa tggagctaag cctagattgg
     9001 cttccgagta gcccattttc tcgtatacaa taaacagatt ggctaacaga tagtcagcta
     9061 attcctcaga ttctttaggt aagacccgca acaaatcata tcccgcctgc ttcttcgtca
     9121 tcggctgagc ggtgatgaag ccaacgactt tttgatcttt acggacaata ccgacaccgc
     9181 tagataatac atatgattta tcaaatcgtc ccacagagaa gtatttttct cgtgcacctt
     9241 gaagccattt ttgtgaaact tcatttacag cttgatgtaa ttcttccggc agttgatcgt
     9301 accaagtaaa atggtatcct tctttcatca aacgttgata ttcaagttgc tgactcagcc
     9361 gagacaagtc ttctgttgct agatcaacaa tccctgcttc acctactttc gtgaagtgaa
     9421 aacccaattc atgaagcatc actacgtagc tttctgatat cttataaaac gcaagctgat
     9481 agcctaaaca atctgcttgt ttcataaact gattcgttgc tgcccgcatc attttaggat
     9541 tgccaactgg atctcctaaa acaaagcatt tgtttccttt gatctgataa ccaaaaacga
     9601 cttgatcttg gtcgtcttct tggtaataaa aataactgta acctgacaag cgaagctgat
     9661 ggcttgtagc tgtgcctccg tatgtttcca ataaacggtc aaaacggtct ttttcgtact
     9721 cactgccaag cttcgtagta tcttcagcta aatattgata aagcgccact aaagctagca
     9781 atgagatgcc caaaccaata agccctgaga accacacatc atctgaagga aacaaaataa
     9841 aatgatttcc tgttattcga ccgactaaaa agcgtccgct gtgatagcct gcaattgcgt
     9901 aaacgataaa caaaaagccg aacaagctag catcaaagat tatagctccc caagaataaa
     9961 cgaattttgt tcgataaaat tcttttctgg ataaccacac agctaacaga atcaagacat
    10021 ataccaatat caactgccaa gataacgtac gtgtgattgt atttaaaata ccaaaagata
    10081 gtaaagcaat cgtaggcaga aatgcctttt tgacttttac tgaaagtgct ctagcaaggc
    10141 cgagtaataa aaagccaacc aatagattca ctgtctgatc taaaaaattg aatgagaacg
    10201 gcaataatac ttgaaatagc cgactgattg cagacagatt agtgattgtt gatagaagta
    10261 ccatcatgat ccccgcaaaa tagacagcac ttacgagcac gatatgcgca accttttgga
    10321 aaaacagtcg cggaatgtta tctaaaaatc gattgatttt gactcctgta tgtgtgatga
    10381 acaatagaag tcccgtcata aaaggtagaa cataataaaa caaccgataa tataatagcc
    10441 aaactactgc tacatcttgc cgaacagaca attgactcat tcctaagatc atcaaaacgt
    10501 caaaagtccc cattccacct gggaccatcg tcagcattcc tatcaatgta gcaatcatga
    10561 acataggata gatcgaaagc atcgaaatat ctattctcat caacttgccg atgatcaaaa
    10621 ataccgcaag ggctcccgtc cattgtccaa aggatgccag tagcagacca agcaagcctt
    10681 ttggataaaa ctcgaagaac aatgtctttc tctttagata agcaaaaaag aaaatgactg
    10741 gtgctaaaaa actaccgccg aaaagccaga tccaatagct tcgaaaaata ctttctgact
    10801 gaataaaaaa gacatcaata aatgtaagaa acgaccatac agacaaacca gtcagcataa
    10861 atagtgcgac ttttgataca gtggctaaaa ccatttttct atttgtctgc tttccataaa
    10921 aactagcccg cagacttgct cctaccacac caccaaaacc ggcgagattg ttgatcgtgt
    10981 ttgttaccca cgcagcaaga agaaactctt ttctttccat ttttggtttt ccttgttttt
    11041 ctaagactct gatcgtcacc caatcataca acaacatcgg agtgacaccc agcagtccag
    11101 ccagaatcat gagcaggatc gtcatgcggc tttgttgtcc catcctttgc caaatatctt
    11161 gccaactcat gccattagca atatttgcga cttgatttgc cacaaagact aacacggagc
    11221 cgaaaaaaat cagttttaaa atcagactat gttcctttat ccattgcagc atcaagtatc
    11281 gtttattttt cacattttcc cattcctaac ttgtatactt ttgtgtcata ccccaatgtt
    11341 atcttattta ttttaatttt tccactttag acctgttctt tgcttgcgat gatagaaaaa
    11401 gaaagatatc ctaatgctaa aggagaggat aacatgaaag taggatttat cggcacaggg
    11461 aatatggcac aagctattat tgatggttta ttaaaaaaag gaacagtctc gaaagaagag
    11521 gtgttcgttt ccagtggaca ttttgaaaat gcacaaaaat ttgctgctga aactggcgtc
    11581 catgcatgca aaagtaatca ggaagttgca tcacaatcag atatcgttct tttagccgtg
    11641 aaaccaaaaa tcattgaaaa agtattggaa gaactaagag aatatagcca gaaagtctta
    11701 ttcatttcta ttgcttcagg aaaatcatta acagatttag aaacagcgct tgaagatcct
    11761 caggcctcca tcgtacgtgt gatgccgaat gtcaatgtca gcgtaggagc aggcgtttct
    11821 gcgatttgca aaaatgaagc tgccacggag gaacagcttc agcaagctac gatgattttt
    11881 gaagcaatcg gcagcgtcta tcctttagca gaatcagatt tcagtacatt cattggcctt
    11941 gctggcagct cacccgcatt cgtttacatg ctgatcgatg cgatgggacg gactggtgta
    12001 ctgaatggtt tgtccaaaca gacagccact gagattgctg caaaagcaat tatgggttct
    12061 tgtcaaaaat tcctttcaag tgataaaaat ccatgggaac ttgtagacca agtttcttct
    12121 cctggcggta caactgttgc aggtgtagtt gccttagaag aagcaggatt catcccagca
    12181 atcatcaaag gaatcaatac gacgatcgag aaagatcttg agatgttgaa taaaaaataa
    12241 tgatagcaat gttttttcgt gccttacaaa aaaagaagag ctgtggcttt tgccgcggct
    12301 cttcttttgt attttttatt atttagcata atcagttgca cgagtttccc gaataacgat
    12361 tactttgatg tgtccaggat aatccagttc ttcttcgatt cgtttacgaa tatcacgtac
    12421 taaacgaaca gcgtctaaat ccgagatttc ttctggcttg accattaccc gaacttcgcg
    12481 tccagcttga actgcgtaac ttgattctac accggcgaag ctgttcgaaa tattttccag
    12541 acttcttaaa cgacggatgt agttttctaa tgattcactt cgcgcaccag gtcttgctgc
    12601 tgagagcgca tctgctgcag ctacaaggac agagataaca gaagtagctt ccacatctcc
    12661 atgatgagaa gcgatcgcgt tgattactac tgggttttct ttgtacttgg cagctaattc
    12721 cgcaccgatc tcaacgtgag atccttcaat ctcatggtca agtgctttcc caatatcatg
    12781 gagcaacccg gcacgttttg ctaattggat atcttcgcca agctctgctg ctaagatacc
    12841 agctaatttt gcaacctcga ccgaatgctt caagacgttt tgtccataac tagttctgaa
    12901 tcgcagacgt ccgatgatct tgattagatc tggatgaagt gtatgagcac cgacttcaaa
    12961 cgctgcctct tccccgtatt cgcgaatacg ttcatccatt tctttacgag atttctcgac
    13021 catttcttcg atacgcgctg gatggattcg tccatcttgt atcagttttt ccaaggtcat
    13081 ccgtgcgatt tctcttcgga ttggatcgaa tccggaaaga acaactgctt ccggtgtatc
    13141 atcgatgatt aaatcgatac cagtcagcgt ctcaagcgtt cgaatattac gaccttctcg
    13201 tccaatgata cgacctttca tttcatcatt cggaagggta acgactgaaa cagtcgtttc
    13261 tgaaacttga tctgctgcgc agcgttgaat cgctaatgat aataagtttt tcgctttgcg
    13321 atctgcttct tctttggcac gctgttcaga ttcttttacc attagagtta actcatgatt
    13381 cagctcttct tcagtcgatt tcatgatgac atctcgtgcg tcctcttttg ataaaccagc
    13441 aattttttct agttcttttt gttgatcttc aatcattttt tcaacgtctt tttctcgctc
    13501 ttcaattaat tgctgttttg aactaagtgc ttcttcttta ctttcaagtg agttctcgcg
    13561 tttttctaac gagtcatctt tacgattcag cgtttgttcg cgttgaatca aacgattctc
    13621 ttgagatttt aattctaact tactttctct cagctcactt tcaacttcag aacggtattt
    13681 ctgattttct tccttagctt ctaacaatgc ttcttttttt agagtttctg cttctttttt
    13741 tgcactatcg atgattccag cagctgatga ttgtgcacca gctatttctt tctcgtgacg
    13801 tgactttgcg accataaacc ctaaaccaag accgacaatt aaaccgatga tagcgaggag
    13861 aatgttgaat accataaaat ccacctccat actatctttt tcatttttat catctataca
    13921 gcttctatga taaattattc taaactcgtc aatatgcatc ttcgcatata tgtgtacaaa
    13981 aaacatgcac aactttattc taatgtttgt accccgtagt gtcaacttta aataaacaaa
    14041 gaaacggctt tctacttatt cattaaacga tggtttttaa aa
//
LOCUS       DAXWOO010000066        14066 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.066, whole genome shotgun sequence.
ACCESSION   DAXWOO010000066 DAXWOO010000000
VERSION     DAXWOO010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.066"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            37..270
                     /locus_tag="ACGE70_001522"
     CDS             37..270
                     /locus_tag="ACGE70_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFF8693164.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(369..800)
                     /locus_tag="ACGE70_001523"
     CDS             complement(369..800)
                     /locus_tag="ACGE70_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFF8693165.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            879..1292
                     /locus_tag="ACGE70_001524"
     CDS             879..1292
                     /locus_tag="ACGE70_001524"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFF8693166.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(1330..2478)
                     /locus_tag="ACGE70_001525"
     CDS             complement(1330..2478)
                     /locus_tag="ACGE70_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFF8693167.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(2600..3196)
                     /locus_tag="ACGE70_001526"
     CDS             complement(2600..3196)
                     /locus_tag="ACGE70_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFF8693168.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(3396..6038)
                     /gene="alaS"
                     /locus_tag="ACGE70_001527"
     CDS             complement(3396..6038)
                     /gene="alaS"
                     /locus_tag="ACGE70_001527"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFF8693169.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            6426..6857
                     /locus_tag="ACGE70_001528"
     CDS             6426..6857
                     /locus_tag="ACGE70_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8693170.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(6902..8251)
                     /locus_tag="ACGE70_001529"
     CDS             complement(6902..8251)
                     /locus_tag="ACGE70_001529"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFF8693171.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(8321..9484)
                     /locus_tag="ACGE70_001530"
     CDS             complement(8321..9484)
                     /locus_tag="ACGE70_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFF8693172.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASRQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            9613..9936
                     /locus_tag="ACGE70_001531"
     CDS             9613..9936
                     /locus_tag="ACGE70_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693173.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(10119..11468)
                     /locus_tag="ACGE70_001532"
     CDS             complement(10119..11468)
                     /locus_tag="ACGE70_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFF8693174.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(11537..12490)
                     /locus_tag="ACGE70_001533"
     CDS             complement(11537..12490)
                     /locus_tag="ACGE70_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFF8693175.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(12592..13782)
                     /locus_tag="ACGE70_001534"
     CDS             complement(12592..13782)
                     /locus_tag="ACGE70_001534"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFF8693176.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
BASE COUNT         4120 a         2925 c         2442 g         4579 t
ORIGIN      
        1 aggctatgat cagactttag aaaggtcgtg agtgctttga ccacaggaat tgtaaaatgg
       61 tttgacaaca aaaaaggata cggctttatc agttatgatg acacagaaga aatttttgta
      121 cattttactg cgattgaaga agagggattc aaaactttgg aagaaaatca agtggtcgaa
      181 tttgagatca tcgaagggaa tcgaggaaca caagctgccc atgtcaagaa aggcaacgtt
      241 cagactaccg atactagtat gcatgaatag ataaacaaat agagtgagac gctaaacggg
      301 atgaaccgta cgtctcactc ttttcttttc ctgtaaaaat tcgttttttt aggttctgat
      361 ttttaaaatt agctttttct ttttttcgta taaggataag gaattgattc tgatttgggt
      421 ccgttttttt caaaatgatc aaaaaggact acttgatctt tttttcgcat catgaaaaca
      481 gatggggctt tctcatcttg caacatcatt tgcaccccag tattagttgg cgtcaaagat
      541 cgaatctgtc cgacagatat ttctcggtct ttccaaaaag cagcataacg tataatcaca
      601 gagtttgatt ttaaaataaa agaacgccgc aaacctagaa atagaaaaac aagaaaaata
      661 cctaaaacaa aattgctttt ccaataagga cctgtgtttt ctaaagaaag aatcagacta
      721 aaaaacagaa tgatcaaggt caatgaccaa tagatgatcg tatgtgccag ttcaggctgc
      781 catcgaatat tttttttcat tctctaacct ctcaccgatt cagtaatgat ataatcttac
      841 cataacatta gtttgaacaa aaaggagaat ctctttttat gttaaaagta tacattgatg
      901 cctctacaaa aggcaaccct ggtcctagtg gaggtggaat cttgattgtc tatcaaggta
      961 aacaagaaca gctatctatc ccgctttcta tagggacaaa ccatcaagca gagttcgaag
     1021 tctttttaaa aactttagaa tttctaaaga aaaacgactt acagaatgaa acgattttct
     1081 gtttttcaga cagcaaaaca ctagtttcta cggtcgacaa aaacgcaacg aagaacgagc
     1141 tcttccttcc ctaccttcaa agaatccaag aacttctatc cgaattttct ttattgatcc
     1201 tgcaatggtt gcccgaaaac aaaaataaag gagcagataa tcttgcacgt caggctctcc
     1261 ataaacaatt aaaagaaaaa aagactcttt aaattaaaaa ctttaaggag tctttttata
     1321 gtttctcatt taatataaaa gataattgtg atcaaacgta atttccaatt ggtaatctcc
     1381 gattttttct tctatttgac tacgtaagat tctttctagt tctgtatcaa caaactggaa
     1441 tttctcaggg accactacat caaattgcag cgtttttcct tgatgaatta ctctaaaatc
     1501 atgaatatta agccctgtac ctgtttcaag aagaatttgc tttaactgcc gcaagatatt
     1561 atagtatttt tcgttatcaa caggcattgg atccaagtga cagacaagtt ccacatccaa
     1621 agacttttta aagtcttttt ctatcagatc aataatctta tgagccacat tcaaattcat
     1681 tgattcatct acttcgatgt ggactgatgc aaagcgttta ttgggtccat agttatgcac
     1741 caataaatca tgatagccaa gtatatcatc gcaagcattt aaatgctctt ccatttcttc
     1801 aatctctgct ggcgttgggc gacttcctaa tagttcgtat acaaaatttc gaagcatcgt
     1861 caatccacta taaaggatat ataatgctaa agcaaaacct atatatccat caatacgcca
     1921 tccagttacc cattcgattg ctgctgaaag caatacagcc agtgtagtaa agacatcatt
     1981 ataactatct gttgctgtag ctctcaatgt ttcagaatca atggattgcg caataacctg
     2041 atacattttg ccttgtgcaa atttcaaaaa gatggaaatg attaggacaa aaaagacgat
     2101 gggtgttaaa gagacatttt ctggatggat gattttttca gcagaagact ctaaaaattg
     2161 aaatcctaca taagtaatga tgatcgaaac aaataaccca ctgatatatt cgaagcgttc
     2221 gtgtccataa ggatgttcct gatctgctgg ctttgcagca attttaaatc cgatcaaagt
     2281 caatatggat gaagcagtat ctgatagact gttcactgca tcagccataa tggagacact
     2341 tcctgaaaaa agcccgatca ttagcttaga gacaaacaag atcagattcg taaccaatcc
     2401 taagataccc gctagtactc ctttttttgt ccgctgttgc ggtatttgtt tctttttttc
     2461 ttcttgtttc ttagccaagt tttttacccc ttctagtttg cttgttgctc aggtaaaaag
     2521 atggagagga cgattgtttt cctctcctct cttccttttt ctatctatcg tgcgtcctat
     2581 tactattaaa agatggtcat tattctggtt tcaacggtgt tgctgcaaat aacgcttttg
     2641 ctagtaatgg tgttatgatt cccgcaccta ttgcttctgg tatcccattg attgctgcaa
     2701 cccctgctaa ggttttaaat aaaagagcag agtctacacc ataggcatct gccgttgcac
     2761 ctgtataaaa taatcccatg aatcctaaca ctaagactgt attgaccaat gaacccagta
     2821 cccctgcaaa ggctgagctg atcgttattg atttattttt ttgataaatc cagtggaaaa
     2881 ccaagcctgc tactaagcca acaagtactc tgggaactac cgagataatc ggattcgtga
     2941 acactaatgt atcaaatggc gttgttggca tggcataggc acgaacaatt gtagcgattc
     3001 cccaaaacaa gccaatcacc atgccatctt ttgggcctaa tactactgca gcaataatta
     3061 ctgtgatatg gataattgtt aaactaatga atcctaatgg aataaacccc agccaaggaa
     3121 ccatcgcttg tacgatgatg attgctaaca agatcgcacg taagactagt cgaaaggtct
     3181 tatttctact attcattttt tcaccccttt ttatttatac taatcgtaaa aatttctgtc
     3241 actttcgtca actaaagacc atccgaatcc gaccatttga ttagatgcgc catccaattg
     3301 aaaaaagtat gagggcaaga cttgtttgtc ttcgccccca taatcatgtt tcgttcaatg
     3361 tttgaacttt tgtttttctg tcaaacgtaa acgccctatt gttgcgcaac ccattctttc
     3421 gcagctttaa gtgcatctgg aataccagca ggatttttcc cgccagcttg tgccatgtct
     3481 ggacgtccac caccgccacc gcctactttc ggtgcgattg ctttaatcaa gtcgccagct
     3541 tttagtccag cttcatttga ttctttcgtc attgccgcta gcagactgac ttttccttct
     3601 tgttcgtttg ctaaaacaag aatatctgaa atattttttt gtttccattg gtcagctaat
     3661 tgacgcagct gattcatatc tttcactttt acttgtgctg caatgattgt ttttccattg
     3721 atcgtttctg gatctttgaa tacatcacca gcttgttgat tcgctaactt gctagctaat
     3781 tgttcatttt ccttttgtag ctctcttact tgctgttgca attgttccgc ttttgaaaca
     3841 acttctttca attgaggtga tttaacaatt gttgctactt gttttagttg tttttcttct
     3901 tcttcaagta gttcgtacgc ttctttactt gttacagcct cgattcgtcg cacgcctgca
     3961 ccaattcctg attcagaaac gattttgaag attccgatgt tttctgtatt tgctacatgt
     4021 gtgcctccgc acagttcgat tgaccagccg ccaatattta ctactcgaac gtcatgtccg
     4081 tatttttcac caaacaaagc cattgcaccc atgtttttgg ctgtatcgat atctgtttca
     4141 attgtttcaa ctgggatagc ttcccagatt ttttcgttga cgattctttc catttcagct
     4201 aattcatctg aagtgatttg accaaagtgg gtaaagtcga atcgtaaatg tccaggagcg
     4261 actaaagaac cagcttgatt tgcatgctcg ccaagtactt ctttcaatgc cttgtgcagt
     4321 agatgggttg ctgtatggtt tttgatgatc cgattgcgca tcgattgatc gatcaataga
     4381 tgataggttt gtccttcact cattggtgct tttacttcta cggtgtgcat aaattgacca
     4441 ttaggcgctt tttgaacatc tgtaacaact gctacgattt cgcctacttc attcatgatc
     4501 catccttgat ccgctacttg cccacccatt tctgcataga aaggtgtttg atcaaagatc
     4561 atttgagctt caccttctgc gacttcattc aaaagagact cacctttaac gatgaccaat
     4621 aattgactat ctgattcttg ttctgtgtat ccaatgaatt tactttctac tttgatatct
     4681 gtcaataaag cagattgtac ccccattgat ttttcagtac tgcgggcaga gcgagcacgt
     4741 tcgcgttgtg catccatttc tttttcaaaa ccagcatgat caactttcag tccttcatct
     4801 tctgccacct cttccgttaa ttctaccggg aagccatatg tgtcgtatag tttgaagatg
     4861 tcttttcctg ctagagtcgt cccgttttcg ttttttactt cttggatcac ttgatttaag
     4921 atttccaaac cttcattgat tgtttcattg aaacgttctt cttctgtacg gataactttt
     4981 tcaataaatt ctttttgttg aagtacttca ggataataac ttaccatgat ttccccaaca
     5041 acagggacta aacgatataa gaatgcttgt tcaataccta attttttccc atgcatgaca
     5101 gcacgacgaa gcaaacggcg taagacatac cctctccctt cattcgatgg caatgcaccg
     5161 tcgccaatag caaatgaaag ggcgcgaata tgatctgcga ttactttgta agaaatatct
     5221 gttactgggt tttcaccata ttttacttgt ttgcttagct tttctacggc gtggataata
     5281 ggcataaaca agtctgtctc aaagttcgtt gacgcatctt gaacgattga gaccatgcgt
     5341 tccaatccca tccccgtatc aatgttttta tgaggaagtg gttcaaatgt gttatcaggc
     5401 ttatggttga attctgaaaa tactaagttc cagatttcta aataacgttc attttctcct
     5461 cctggatagt tttcaggatc gtcttctgct aaatcgttat aagcttctcc acggtcatag
     5521 aaaatttccg tatccggacc acatgggcct gcaccgatat cccagaagtt gtcttctaca
     5581 tcaataatgt gatctagtgg caggccaact tcttcatgcc agatgcgttt tgcttctgta
     5641 tcttttggat aaactgttac ataaagtttt tctggatcga atgccatcca ttttggatca
     5701 gtcaaaaatt cccatgccca gtggatggct tcatttttga aatagtcacc aatcgaaaaa
     5761 ttacccagca tctcaaacat cgtatggtgc cgtgctgttt taccgacatt ttcgatatca
     5821 tttgttcgaa tagatttttg ggcatttgtg attcttggat tttctggcac aactgaacca
     5881 tcaaaatatt ttttcaatgt tgcaacacca gagttgatcc acaataaggt tggatcgttc
     5941 acggggatca aagacgcact aggttcaatc gtatgtcctt tagattggaa gaaatcaaga
     6001 aacatttgtc gaacttgtgc actacttaat tgtttcatgt cattacctgc tttctataaa
     6061 ttaaatacaa aaaaacacca tcacagccaa ggacgaaaac tcgcggtacc accttgattg
     6121 caatgatgtg taatcattac ctcttaaact cgataacgct gagctgcgtc ggtatttcta
     6181 ccaaaataga aaaagggagc aatttgatta gtgactattc ttttttcagc caagaaagaa
     6241 ttttctgtga gtcttaaaaa acaaatcata tccttttcgt acttaattag tatacgcatt
     6301 ctattgatca ctgtcaatat ctcaattttt attttcattt ccttttggtc atactccacg
     6361 atttttggta cgataagaat aaaggaatca atacatctct gctattcatg acaaggagga
     6421 atatgatgaa aaaaatagtt ggagcggatg aatggatacg cgcagctagc tgctttttga
     6481 gatatcaagt ttttgtatta gaacaaggga ttcctgcaga actagaattc gacggaaaag
     6541 atatcccgca acaaacctat tatctatttc ttgatcaaaa aagaccagta gctactttgc
     6601 gttatgaaaa gaaaacacct acttgcttca agccggaacg attttgcgta gcaaaagaat
     6661 accgaggaaa agggttaggc ggtcagttgc ttgaagctgc tgaaaaacgg gcggtttcag
     6721 aaggttgtca gactagttat gtagtagctg aaatgacggc tgtagatttt tatgaaaaac
     6781 aagggtatca tacttgcaca gaacctttta ttgaagatga agtactttgt atcggaatga
     6841 aaaaaagtct agcttaaaaa caagagattg tgacacgcct tttgtcacaa cctcttgttt
     6901 tttatttatg tgaatttttc ttatttttac gagcagtacg gctttgctgt ctgcgttcga
     6961 ttttgcgttc ttttttattt ttttctgcaa ttgcccaatc gatttttttc ttataaccag
     7021 gcttgatctt tttctttttc ttcttcacaa gaccaatcag cgtgggatct aatacatctt
     7081 ttgatttttc tcgtttggca cgtctattac gatcgtatgt tttgacgatt tccccgtttt
     7141 tgatttcttt tggttcaaat gagacgccca atttttctat cgcatcaatg gcttgctcat
     7201 catttggcga atataacgta atggccgttc cttttaagcc attacgtcca gtacgtccga
     7261 cacgatggat aaagaagtct agatcttctg gaatttctgc attaataaca tgagagacac
     7321 cttcgatatc gatccctcta gcagctaaat cagtcgctac aacatattga tactccagat
     7381 tctgtacttg tcgcatcact cgttttctct ctcttggggt gatgtctcca tgaatctttg
     7441 ctactttcaa gccttggttt ttcaagtaat cggcaatttc atccacatgc tgttttgtat
     7501 tggcaaatac gattgctaaa taaggatgac caattgtcag cagttgataa atcatttgat
     7561 tctcatcttt accttttgtt gaaatcagcc aattttcaat gtcttcagaa ataactgctt
     7621 ttggtttgat ttcttcaatg attggatttt ccatgtattt tcgtaagaat ggacgaagtt
     7681 tttctggaat agtggcagaa aaaactaaaa actgcagctt ttctggtaat cgacttgcaa
     7741 tttggtctac ttcatttaaa aaacccatat ccaatgtcat atccgcttca tcgacaacaa
     7801 atgcaaatgc ggtgtgaatc ttcagcgctt gttcattcat catgtctaat attctaccag
     7861 gcgtgcctac tacaacctgt ggctgttggt gatgcagacg atttaattga cgctgtttgt
     7921 ctgtaccgcc gacaaaattc gagacacgga tctccggtgt agaaaattta gcgatttgtg
     7981 tagctgcttc atagatctgt gcagctagtt ccctacttgg agctgtaata acaacttgta
     8041 cttcctcttt ttctgcattg attttttcaa acaaaggcaa caaaaacgta tgtgtttttc
     8101 ctgtaccagt ttgagattgc ccaatgatat tttttccttt tgcaattaaa ggaatcaaac
     8161 gttcctggac ctctgtgggc tgtttgaagc cctttgcggt taacgcttca ttgataaacg
     8221 gtttgaagcg gaatatatca aaattattca ttcgtgcacc tcttctcttt aaattatcac
     8281 gactttggca ttataacaca gacaaagcgt ttttgctcag ctaatttcga gaatcatcat
     8341 cctttgtttg gccaatcgtg ctaacagtat atgtgtgact agctttttca taaaacagaa
     8401 tccgtttttt tctcaaaaat gatagcaaga tatttaaaat aaaaatacct tgtagtaaca
     8461 agacaccgag agaaatgaat actgcactca tctgcaacac cgtattcgtt gtcgaattca
     8521 tgatttgctg aagcaactgc cccaaccagc ttaaaccata aaaaagaaag tacagatacc
     8581 cgtagcggat catcaatgct tttaaagaaa ttcgttcgcg gtcttcttcc accaaacgga
     8641 tacgcacaat ttttttgcca agtgtccatc cattggttat aaacggtaca atcataaagt
     8701 atacccctac ttctaataaa agccaccaga tatgagaagt aactgcccct gaatcgaacg
     8761 caattttaaa gaaaatcgcc gctaccaaat taataagaga taaaaatgtc cagtcgatca
     8821 aataagcgac caagcgtcga gtcaatgaga cagtctttcc tttttcataa gaaagttcat
     8881 ccattcgtct tcttgtagga agcataaatg taaataacgg cgtaacgaca aaaccaatga
     8941 ttccacccaa tgtatttgtc actaaatcat tcacatcaaa taagcggtat ggtcttgggt
     9001 agataaaata aagtccactt agctgcgtca attcaaaaaa tagagaaagg cagaaactag
     9061 caatcatcgt tttcataaaa gaaagccgga aataatatct taaataaatg ccaaatggta
     9121 tgagtaataa aacattaaaa gctggttcca aaaatacact ctgcttcatt gccggcaggt
     9181 aggtactagg ttcagacaag accaataccg tgttgtctct gaaatgttgc cttgaagcaa
     9241 ataaatgcag ctccatcttt ggccctgtca tttgggcaac cgtctccttt gccggaagtg
     9301 gtaagatcac caaaaaatac gcacacaata aataaaaaac gaaagaatac aaaataactg
     9361 cccttgttac tagaaatgtt ccatactttc gatattcata aataaaaaaa acagatgaga
     9421 tcgctaaggc taaaaatgga aaaacaatca gcgcaaaacg aatcggttcg atataacttg
     9481 ccatatgact cctccttgat tctttgtatc tctcttatct tagcagtcct ttgctgtttc
     9541 aagcaagtaa cgaggctatt atttctcatt tctttcaaag taaagtaaga taaacacaaa
     9601 gggggtgtcg agatgctgaa tatgtacttt gtttttggcg tacctatttt tttactgttc
     9661 ctttatgcta cgatcgctta tgtaagaaaa agaactacaa ttcattattt aggatttatt
     9721 ctcctgatta tttcgggttt catgctagtc ttcaatcttc agacttggca gcaagcactt
     9781 cttgaaatgg ataaaatgac tccacatgct ttgtctaaag tgcttggcta ccctgtctat
     9841 ctgatttggc ttcccatttt catttctggt tgcttggttt tattaaatat ttatcgcggc
     9901 gtacgccgaa tagttcagtt acgcaaatca aagtaaaaga cagaaatcgg ttgagcccga
     9961 tttctgtctt ttactttgct atttcatcgt agacaaaacg acatttacgt gatcaaaatt
    10021 aacgagtact tttgacattt cttccacgta ctcctcattt aaataaaata ttttgtttgg
    10081 cagagaagtg cagttctaat aaaattcgaa tgttttagct atctacgttt taaatagaga
    10141 taaatcggga taccagcaag tgtcaatact atccccatcg aagctaaaaa ggtctgagtg
    10201 ataagcgtgc ttgatacgat aaaagtccct ccaatgattg ctactagtgg aataaacgga
    10261 tacattggaa ctttgtatgg tcgaaacaaa tcaggttctt ttttacgaag aagaatgaca
    10321 ccaacaaaga ccatcgtata aaaaatccaa atgacaaaga tcagcatatc cgttagtgta
    10381 tcaaaaccac cgatcatcat catgccaata gcgatgacta gctctaagat acctgcggct
    10441 acaggaactt tagttttatc atgaagttta gcaaagagct tgctaaaagg caggttgttt
    10501 tcttttgcca taacataagg gagacgcatt ccagtaaggg tatagccatt gatggttcca
    10561 taaacagaaa tcaagatacc aactgtgacc agtcttccac cgaagccgcc aaaaatcatt
    10621 tttgcaacgt cacttgccgc attgctgttt cctgcgactc cgtcgataga tgcggttctt
    10681 aagaacaccg catttactaa taaataaacg atcatgattc cgatgattcc taaagaaatt
    10741 gcttttggca aatctttcgc cggctttttc aattctcccg aaatatttcc tacgtgtatc
    10801 caaccgtcgt atgcgaacat cgttgctagc aagcccgctc ccaaggctga gaaaaatgaa
    10861 agattttctc ctgcttggat cggaaacagt tggaagtcta ctccttcttg tcggaacaaa
    10921 ccgaaaatga cgatcacaaa caaaggaata agcttgcaga caagcgtaat cgactgaaaa
    10981 gcccctccag ctttagaacc taaaaaattg atcaacagga tcgatacagc agctgtaaca
    11041 gctacgggaa caatcattga ctgggaaaga ccaaatagat tgacgaactg tgtaccgaaa
    11101 atgatcgaca aggctgctac atttgctgga aaataaatca cgacttgcgc ccagcccaat
    11161 aaaaaagctg ctgtatttcc gtaaatccgt tcgatatatt taatcatccc tcctgtttct
    11221 ggaatagcag ctgccagttc agctcctgtt agtcctgcac agacagaaat cataccaccg
    11281 agaaaccaag aaaacatatg caaactggcg gatcctgtaa cttctgctac gcttgcggct
    11341 ttaaaaaata cccctgcacc gatcactgtg cccataacag tcgataaagc agtaaaaaat
    11401 cccatcgtac gttttaattc attttctatt tttacttctt ttactggcgt ggcttgatat
    11461 tcttccatcc cttgtcccct ttcataccat agccaatccc aaaagtataa aaaacttttt
    11521 ggatgtggtt cttgttttat accgtctcta ccaatttttc tttcagtttt tccaacatat
    11581 cctttgtcat cacatctaag tcgtatttcg gcgaaaagcc ccattcctct tgcgcacaag
    11641 aaacatctag tgaatttggc caagattctg ctattgactg acgtacagga tccacatcat
    11701 aatccatttc aaaatctggt atgatttttt gaatagatgc tttgatctct tccgggtcaa
    11761 aagacatcgc cgatatattg aaggcatttc gatgaaccaa tcgatcagga tctgcattca
    11821 tgagttttat aatggcatcg atcgcatctg gcatatacat catatccatt gatgtacctt
    11881 gatcaatgaa actagtgtac tttccttctt tgattgctga ataataaata tcgactgcgt
    11941 agtctgtcgt accgcctcct ggcaaggttg cataagaaat caatccaggg aaacggacac
    12001 cacgagtatc tacgccatat ttcacataat aataatcaca tagtagttct ccagctacct
    12061 tcgttacccc atacatggtt gtaggacgct gaagagtatc ttgtggggta tgatcttttg
    12121 gagtatttgg accaaacgcg ccgattgaac ttggcgtaaa aaatttcagc tggaattctc
    12181 tagcgacttc caatgcattg accagcccag tcacgttcaa attccaggct aattgtggtt
    12241 ttgcttctgc aacagctgac aaaagagcag ccaaatgaat caatgtgtcc acttgatatt
    12301 gttctgctat gctgcgcatt ttttcgtaat ccattacatc taggatctca aagatatcgt
    12361 ttttctcaat gacttcaggc atgcgtatat ctgtcgcaat gacattttca ctgcctagtt
    12421 cttgacgtag ccgtgtgatc aactctgaac caatttgtcc taaacatcct gtcaccaata
    12481 cttttttcat gatgatcctc cctgatcttt tttactacta tcttcagatg attaacaata
    12541 tatgcgtgaa catgttctct aattttaaat agacgaaaaa actaaattta gttaaataat
    12601 ttccatttct tttcctattt tttgataaac tgctactgct tcatcaagca tttccctcgt
    12661 atgttccgca gtcggcatat ttctgatcct gcctgtccca agaggcacag ttggataaac
    12721 gattggttta gcataaatcc catgttcgat cagcttctta gagaaagtct gagtcacttt
    12781 ttcgtctccc aaaatgactg gagtaatagg tgtttcagac ataccgatgt tatagcctac
    12841 ttttttcaat tcttctttaa aataattagc attttcccaa actttttcga caagttccgg
    12901 atgttcttgc atcaaagtaa tcgaagccaa tgcagctgca gctgcacctg gagttagcga
    12961 agtagaaaat aggaatggcc gacttctagc tttcaaccag tcgattagtg ttttcgatcc
    13021 agctacataa cccccaacaa cacctatagc tttcgataac gtgcccattt gcatatcgat
    13081 tttgtcgctt aatccaaaat gcttgacggt tcctgcacct tttccagtta cgcctgagcc
    13141 gtgcgcatca tcgacgtagg tgatcagtcc atatttttca acaatcggaa taatttccgg
    13201 caatcgtgca atgtctccat ccatggagaa aacaccatct gtgatataca tgattttttt
    13261 atatttctta ctttctatcg cttcctttgc ttttttctca aggtctttca tatcttgatg
    13321 tttaattcgg atgatttttg ctccggataa gcggcaaccg tcaataatag atgcatggtt
    13381 taattcatcc gataagatag catcttcttt tgtcattact gcagagatag cgcccatatt
    13441 gcagttgaat ccagattgaa aggcgattgc tgcttctgtc cctttgaatt ctgcgatttt
    13501 ttcttctaat tgctgatgga ttttcagcga accattgatc gttcggactg ctcctgctcc
    13561 tacaccataa gtttcggttg cttcaataca tgcttttttc aattcttcgc gattggcaaa
    13621 tccaagataa ttgttggatg ctagattgat catctttttt ccgtctacga tgatacaagc
    13681 accattttca ctttctagta tgtcgatcgt attgtataac cccttttctt tcagttgatc
    13741 caattctttc tctaaataca ttgttagttc tttggaagac attgtctcca cctccatatt
    13801 catctgtctt gcagaacata ccaaacatat atcgtgtcat tcatctacaa agcaagtatg
    13861 ctatattttt gataacgctt acactgatta tgagaattat ttaaccacat tttttattta
    13921 cagtatcttt ttactttcaa atgacttata tagtatgaac aattaccttt tagataatga
    13981 tctattttac tctacctggg cagataaata caacacaacc attgatagaa gccctttcgt
    14041 taaaatcaat tgtattctga atattt
//
LOCUS       DAXWOO010000067        13687 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.067, whole genome shotgun sequence.
ACCESSION   DAXWOO010000067 DAXWOO010000000
VERSION     DAXWOO010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.067"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(32..3685)
                     /gene="rpoC"
                     /locus_tag="ACGE70_001535"
     CDS             complement(32..3685)
                     /gene="rpoC"
                     /locus_tag="ACGE70_001535"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFF8693177.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(3764..7381)
                     /gene="rpoB"
                     /locus_tag="ACGE70_001536"
     CDS             complement(3764..7381)
                     /gene="rpoB"
                     /locus_tag="ACGE70_001536"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFF8693178.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8109..9077
                     /locus_tag="ACGE70_001537"
     CDS             8109..9077
                     /locus_tag="ACGE70_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFF8693179.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9095..10000
                     /locus_tag="ACGE70_001538"
     CDS             9095..10000
                     /locus_tag="ACGE70_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8693180.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            9997..10803
                     /locus_tag="ACGE70_001539"
     CDS             9997..10803
                     /locus_tag="ACGE70_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8693181.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            10960..11928
                     /locus_tag="ACGE70_001540"
     CDS             10960..11928
                     /locus_tag="ACGE70_001540"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFF8693182.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12245..12769)
                     /locus_tag="ACGE70_001541"
     CDS             complement(12245..12769)
                     /locus_tag="ACGE70_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFF8693183.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13056..>13687
                     /gene="scm"
                     /locus_tag="ACGE70_001542"
     CDS             13056..>13687
                     /gene="scm"
                     /locus_tag="ACGE70_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFF8693184.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFL"
BASE COUNT         3843 a         2961 c         2709 g         4174 t
ORIGIN      
        1 ataatttgtc acaacctctt ttttaaaata tctattttgt ttgattgttc attgcgtctt
       61 ctgcagccat ttgcgcttca atatctgaaa tactgtagac attttcgctt gctacaccga
      121 cttcttttgg ttccatgtca cgataacgtt gcataccagt accagctggg atgatcttac
      181 ctataataac attttctttc aaaccaagaa gaggatcttt cttaccgcgg atcgcagcat
      241 ccgtcaagac acgagttgtt tcttggaatg atgcagcaga taagaaacta tttgtttcta
      301 atgatgcttt tgtgatacct agtaagacag gtcgagaagt tgcaggaacg cctcctgaga
      361 ctagcgtatt gtagttttga tctttaaagt ctgcgatgtc taataatgta cctggtaaga
      421 tatctgtatc acctggatcc attacacgaa ctttacgcag catttgacga accattactt
      481 cgatatgctt gtcgccgatt tctacccctt gcatacggta aacacgttgt acttcacgaa
      541 gcaagtagtt ttcaacagat aatacatcgc gaacttgcaa caattgtttt ggatcgattg
      601 atccttctgt caatggtgca ccacggtgga tcatatcgcc ttctgctact ttcatacgtg
      661 ctgtataagg aacagtatac gtacgtgtat ctgtttttcc tttgattgtt acttctttgg
      721 tacgtgttgc aggatcttct gagatatcga tgacatcccc tgttacttct gtgatcactg
      781 cttgcccttt cggattacgt gcttcaaaga tttcttggac acgaggaaga ccttgagtga
      841 tatcgtctcc ggcaacccca cctgtatgga atgtacgcat cgtcaactga gtaccaggtt
      901 caccgattga ttgagcggcg atcgtaccaa ctgcttctcc gacttcaacg tcagagccag
      961 ttgcaaggtt gcgtccgtaa caatgtttgc agacaccatg ttttgtatta catgtgaaga
     1021 cagaacggat tgtcactgtt tcgacacctg catcaacgat ttgttttgcg atgtcttcgg
     1081 aaatcaattg gtttcctgca atgatcaatt catttgtttc aggatgacgt acttctttac
     1141 gggtgtaacg tcccactaaa cgatcttcta atgattcgat gatttcattt ccctcgcgga
     1201 tggcttggat ttcaagacct cggtcagtac cgcaatcttc ttcacggatg attacatctt
     1261 gggccacgtc aaccagacga cgagtcaagt aacctgaatc ggcagtcttc aaggctgtat
     1321 cggtcatccc tttacgagcc ccgtgagttg agataaacat ttccaagacg gatagtcctt
     1381 cacggaagtt cgaaatgatt ggcaattcca tgattcgacc attaggagcg gccatcaaac
     1441 cacgcattcc ggcaagctgt gtaaagttgg aaatgttacc acgagctcca gaatcagaca
     1501 tcatgaagat tggatttttc gcttccaaac cttccatcag tttttgttgg atgctgtctt
     1561 ttgcagcatt ccaaacagca atgacacgtt cataacgttc gtcatctgtg atcagaccac
     1621 gacggaattg ttttgtaata gtttcaactt gtttatgggc attttcaatg atttcttgtt
     1681 tttcattcag tacactgatg tcagcgatcc ctactgtgat accggcatgt gtcgaatgtt
     1741 tgtaccctaa gtctttcata cggtcaagca tcttagaagt ttctgttact ttgaatcgtt
     1801 tgaagacttc tgcgatgatg tttcctaagt ttttcttctt gaacggtaat actaattctt
     1861 gctctttgat atgtgcagga atatcagtac cagcttcaac aaagtatttg tccggtgttt
     1921 ggacggtcaa gttgaagtct gttggttcat tcagataagg gaattcagga ggcatgatct
     1981 cgttgaagat gatcttacca actgttgtga tcatcgtacg ttctttttgc cattcagtaa
     2041 atggtttttc acctaatgag ttagggttca caccgatacg tgaatgtaaa tgaacatatc
     2101 cgttgcgcca tgcgataacg gcttcgtttt gatctctgaa gaccattcct tcgccttcgc
     2161 gtccttcttc ttccattgtc aagtagtagt tacctagaac catatcttga gaaggagtaa
     2221 caactggttt accatcttta gggttcaaga tgttttgagc agctaacatc aacatacgtg
     2281 cttctgcttg tgcttcttca ttcaatggaa cgtggacagc catttggtct ccatcgaaat
     2341 cggcattata agcttcacat acaagtggat gcaaacggat tgcgcgtcct tgtactaaaa
     2401 ctggttcgaa tgcttggata ccaagtcggt gaagcgtagg tgcccggttc agcaatactg
     2461 ggtgttcctt gattacgtct tctaaaacat cccatacttc gtcttcttga cgttcgattt
     2521 tacgtttcgc atttttgatg tttgaagcta gttcgcgttg aacaagctca cgcatcacga
     2581 atggtttgaa taattcaatg gccatttctt ttggaagtcc acattggtac atcttcaaga
     2641 aaggtccaac aacgataacg gaacgacctg agtagtcgac acgtttacct agcaagtttt
     2701 gacggaaacg accttgtttc cctttcaaca tatgagaaag agatttcaat gggcggttac
     2761 ctggtccagt aactggacgt ccgcgacgac cgttatcgat caatgcatct actgcttctt
     2821 gcagcatacg tttttcattt tgaacgatga ttccaggtgc attcaagtct aacaaacgtt
     2881 tcaaacggtt attacggttg attacacgac ggtaaagatc attcaagtca cttgttgcaa
     2941 aacgtccacc ttctaattga accattgggc gtaaatctgg tggaataaca gggataacgt
     3001 ccataaccat ccagcttggt tgatttcctg aagcgcggaa tgcttctaaa atatccaaac
     3061 ggcggattgc acgtgtacgt ttttgtcctt gtgcagattt caattcttct ttcaattcgg
     3121 ctgcttctgc atctagatca acattatcaa gtaattgttt aatcgcttct gcacccatag
     3181 cagcttggaa ttcttgtcca tattgttcac gtttttcgcg atattcgcgt tctgtcaaca
     3241 attgtttttt ctctaaattc gtatttcctg gttcgatgac tacatatgaa gcaaaataaa
     3301 tgatttcttc caaagcgcgt gggctcatgt ctaatacaag acccatacgt gaaggaatcc
     3361 ctttaaagta ccagatatgt gaaacaggag ctgctaattc aatatgtccc atgcgttcac
     3421 ggcgaacttt cgaacgagta acttccactc cgcatcgatc acaaacgatt cctttataac
     3481 ggatacgttt gtattttccg caggcacatt cccagtcttt tgtaggacca aaaatccgct
     3541 cacagaacaa cccttcgcgt tcaggtttta aggtacgata gttaatcgtc tcaggttttt
     3601 ttacttcacc gtaagaccag cttctgattt tttcgggaga agccaaacct atttgcatac
     3661 tttcgaattt atttacatcg atcaaaaggg gttccctcca tttcatttaa tggcaaaccg
     3721 caagacggct gatcttcgac cgccttgcag ctcatagacc ggattagtct aaattatctt
     3781 ctgcggtttc aaagttttgg atgacatcgt ctttttcatc ttccacttgt tcagcagctt
     3841 tcttttctaa ttctttggca gtttgctgtt cggcaaattt tgtcaaagca tcaacagtga
     3901 tcaaatcgtc gtcttcatcg tccatgtcgc gaagttcgat ctctgtttct tcgatatcca
     3961 agacacgcat gtccagacct agtgattgca attctttcac caatacgcgg aatgattccg
     4021 gtacacctgg ttttggaatt ggttcaccct tgacgattgc ttcgtatgtt ttcacacgtc
     4081 cgactacgtc atctgatttg tatgtcaaga tttcttgtaa agtataagcc gcaccgtaag
     4141 cttccagtgc ccatacttcc atttcaccaa aacgttgtcc accaaattga gctttacctc
     4201 ccaatggttg ttgtgtaacc aatgagtaag gtccgattga acgtgcatgc aatttgtcat
     4261 cgaccatgtg agcaagtttg atcatgtaca tcacaccgac agacacacgt ccatcaaatg
     4321 gttcacctgt acgtccgtcg tacagaacag ttttcgcatc gctggccata ccggcttcac
     4381 gaactgtttc ccatacgtct tcatcacttg caccatcaaa tactggcgtt gctacgtgga
     4441 tacctaactg acgtgcagcc atacccaagt gtaattccaa tacttgcccg atgttcatac
     4501 gtgaaggcac ccctaaagga ttcagcatga tatcgattgg tgttccatca ggtaggaatg
     4561 gcatatcttc ttccggcata atacgggata caaccccttt atttccgtga cgaccggcca
     4621 ttttatcccc ttcatggatt ttacgttttt gaacgatata aacacgaact aacatgttta
     4681 cgcctggtga caattcatct ccagcttcac gagtaaagat tttcacatcg tgaacgatac
     4741 cgccgccgcc atgtggtaca cgtaatgatg tatcgcggac ttcacgagct ttttcgccaa
     4801 agatcgcatg caatagacgt tcttctgcag ataattctgt tacccctttt ggcgtgactt
     4861 tccctacaag caaatcgcca tcttggactt ctgcaccaat acggatgata cccatttcgt
     4921 ctaaatcttt taatgcgtct tccccgacat tagggatttc gcgtgtgatt tcttcaggtc
     4981 ctaattttgt atcgcgggct tctgattcat attcttcaat atggatagaa gtgtatacgt
     5041 catcttttac caaacgacgg ctcatgatga tcgcatcttc atagttgtac ccttcccaag
     5101 tcatgaaacc aactaaaacg ttttggccta gagccatttc gccttgttcc atagatggtc
     5161 cgtctgccag agtatcccct ttttcgactt tctcacccaa atgaacgatt ggacgttggt
     5221 tgtagcttgt tccggagttt gaacgacgga attttgttac atcgtattta tctaatgcac
     5281 cgttgtcgcg gcgtacgcgg atttcagaag catcaacgta ttcaacgaca ccgtcatgtt
     5341 tacataacaa agcagctcct gagtcatgcg ctgatttgta ttccatacca gtaccaaccc
     5401 atggtgcttg aggattgatc aacggcactg cttgacgctg catgttggca cccatcaagg
     5461 cacggttgga gtcatcgttt tccaagaaag gaatacatgc tgtcgcaacg gcaactactt
     5521 gtttaggtga tacgtccatg tagtctactt tatcaataga tacttctaag ttttcacttt
     5581 gagcacgagc cataacaacg tcttgagcaa atgttccatc ttcattcaat gggctgtttg
     5641 cttgggctac gatgtaatgg tcttcgatat cagctgtcaa gtagtcgatt tggtcagtaa
     5701 cacgacctgt ttcacggtct acacgacgat aaggtgtttc gataaatccg aatttattta
     5761 ctttcgcata actggacaaa ctattgatca acccgatgtt tgggccttca ggagtttcga
     5821 tcggacacat acggccatag tgggagtagt gaacgtcacg aacttcataa ccggcgcggt
     5881 cacgagtcaa tccgccaggt cctaaggctg aaagacgacg tttgtgtgtc aattcgccaa
     5941 gagggtttgt ttgatccatg aactgtgaca actgagaaga accaaagaat tctttgatac
     6001 ttgccacaac tggacggata ttgatcaatt gttgaggtgt caatgtttca gtatcttgga
     6061 tcgacattct ttcacgaacc acacgttcca tacgtgctag cccgatacgg aattggtttt
     6121 gtaaaagttc accgactgaa cggatacgac gatttcctaa gtggtcgata tcatcgacat
     6181 tgccgatacc ttccatcaag ttcaagaaat agctcattga agcaatgatg tcggctggac
     6241 gaaccgtttt tacagctgct tctggatagc cgtttccgat cacgtttact tcacgttcag
     6301 ggtctcttgg tgaaaatact ttgatgactt ggacagtcat tggatctgtg accacaccgt
     6361 cttctgatgg atagtaagtc actgaattta ggccattatc aatgaaagga gctagtgttt
     6421 ccatgacttg gtgagtcaga actgttcctt tttcaacgat gatctcgcct gtttctggat
     6481 caactaatgt ttcagctaat gtcagattca atagacgtgt tttcaaatct aattttttgt
     6541 ttactttgta acgaccaacg tttgctaagt cgtaacgttt tggatcgaag aaacgagcat
     6601 tcagcaagtt acgtgagctg tctgctgttt ttggttcgcc tggacgcaaa cgttcgtaaa
     6661 cgtctttcaa accttcttct gttcttgagt cgcttgcgtt tttatgcaag tctttttcga
     6721 ctgtattgcg taatgtttcg ctgtcgccga agatttcaaa gatcgtatca tcggaaccaa
     6781 atcctaacgc acgaactaat acggttaaag gaatcttacg tgtacggtcg atacgtacat
     6841 aagagatgtc ttttgcatca gtttccattt ctagccaagc tccacggtta gggataacag
     6901 tagaaccgaa tccttctttt ccgtttttgt cgacttttcc atggaaatag acacctggtg
     6961 aacggactaa ctgagaaacg attactcgct ctgccccgtt gatgatgaat gttccttgtt
     7021 ctgtcattaa tgggaaatca ccgaagaata cttcttgtgc tttgatttcg ccagtctcgc
     7081 ggtttgtcaa acgcaatgtt acatgtaatg gtgcagagta gtttgcatca tgcgcacgag
     7141 cttctgctac tgtatacttt ggttctttca attcataatc tacaaattcc aaagatagat
     7201 tgccattgaa gtcatcgatt ggcaaaatgt cttcaaacat ttctctaagt ccctcatcta
     7261 agaaccattg gtaagagtct gtttgaattt cgatcaaatt tggtaattcc aacacttcac
     7321 tgattcttgc gaaacttcta cgttcgcgat gttttccgta ttttactacg tgtccagcca
     7381 agctcttcac ccctctaaaa tgtcattcaa aaagactacc cgatcaattg ttacaataat
     7441 atttcaaata ttatttttag gtaacaacag acattttttg cggcttttag gcaaaaaaaa
     7501 accaaaaata gaacattctc ttgaaacctt acttcgtgag cttctacttt tgtttcctta
     7561 tattaaagcc gaaacggaca atatttcgtt tctgcctttt cttcatcaga tagtttttgc
     7621 aacttcatat actacactta ttttctataa aagtcaacac ttcctggcgt ttcttctgct
     7681 ttttcgcaac attttcttaa tgttttcaat tttatgtttt caattatctg acaattctta
     7741 attgacagac ctttttatct cggttatact ttagaaaatt cattttgtgg aggaacttct
     7801 atgcgaacca acatcaacca aatcgcacca acacaactga ataactttta ctttagctac
     7861 tttagatagg tttgtattca tcatgatgga tacaaacgtt aaacgacagt ttgtatctat
     7921 gatggctaaa gaactttgta ttgcagcaaa aagccacgta gacaaaatgc taagtggctg
     7981 agcagtgaaa atacaaagta aaagtatcaa gcattctact ttttcaattt attttccgac
     8041 ttttcaatcc atttggacat tttgtctgaa tggatttttt gtttcccaaa attaaggagg
     8101 aaaaagaaat gaaaaaaaat ataatcatcg gattaggcgc tgccctgcta ctagctttag
     8161 gaggttgcgc acaaaaagac aattccgcag aggcatcaga tggacaaaaa acaattggcg
     8221 tattgcaact tgtacaacat aactcgttag atgctgccta taaaggattc aaagaaggat
     8281 tagcagaaga agggtataaa gaaggcgata atctaacaat cgattatcaa aatgcccaaa
     8341 acaatcaaga caacctgaaa agtatgagtg agaaactcgt caaagaagat cctgatttgc
     8401 tacttggaat cgccactcca gcagcacaat ctttattaaa tgaaacaacc actatcccga
     8461 tgactgtcac agcggtcaca gacttggaac aagcccattt agtcacatcc aatgaaaaac
     8521 ctggcggcaa tgtgactggc acgacagata tggtgccaat cgaaaaacaa atcggcttac
     8581 tgttaaatat cgtgccagac gccaaaacaa tcggcatcat gtataacaac ggtgaagcaa
     8641 actctaagat ccaagctgat ttggcagaaa aagaattgaa aaaagcgggc gtcaaagtca
     8701 aagtactgac agcaaattcc acaaatgatg tccaacaagt gacgaaaagt ctagctaaag
     8761 atgtagatgg aatctatatt cctactgata acacgttcgc aagtgcggca agtgttgtcg
     8821 gtgaagtcgc aaaagaaaca aaaacacctg tcgttgctgg cgcagttgat caagtcaaag
     8881 acggcggtct agcaacagta ggaatcgact acgaaagctt gggcaaacaa acagggatca
     8941 tggcagcaaa aatcttagac ggcaagaatc caagtgaact tcctgtcgaa acagcgaatg
     9001 atttggaact gtatgtcaat aaagacatgg ccaaagcatt aggcatcaat ccagacagca
     9061 tcaaagaagc tgaataaaac acaggaggat tctcatgaca aatttgctct ctcttttctt
     9121 atcatctgca tcgcaagggg tcttatgggc cctacttgcg attggtgtat acgtcacttt
     9181 ccgtatcttg gacattgccg acttgactgc tgaaggaagc tttcctttag gtgcagggat
     9241 cgcggcagta agtattacaa atggctatca tccattgaca gcttgtcttc taggcttttt
     9301 agggggtgcg gcagcagggc ttgtttccgg actgcttcac acaaaattga aaattcccgc
     9361 ccttcttgca gggatcatca ccatgactgg tttatattct gtcacatccc gtgtgatggg
     9421 tgcgccgaac atctctttat tgggtcagcc aacgatcttt cattgggtcg agtcattcgg
     9481 cataacaaaa gacaacgctg tactgatcgt cggtctgctt tttgctttga tcgttgtatg
     9541 tttacttgtt ttgttcttac gtacagaaac aggacttgct attcgtgcaa ctggcgataa
     9601 tctggcaatg agcgaagcaa acgggatcaa cacagataat atgaagatca tcggttatat
     9661 gatttctaat ggatgcattg ctctttctgg ctccttactt gcgcaaaaca acggctttgc
     9721 tgatttaaat tctggtattg gaacgatcgt aattggactt gcttctatta tcatcgcaga
     9781 agttttgtta cgtaaccagc cactatttct ccgtctcttg actgtcgttt taggtgcggt
     9841 catttatcgt tttattttag cgttagtctt cgaactgaat gttgaaccaa gtgattcgaa
     9901 acttgcttct gctttcgtgc tagtgatctg tctttctttc ccgcagatcc agcaaaaatt
     9961 ccatttacct caaaaaaaga aaggcggtag tgtcaaatga atgacgtctt aacattggaa
    10021 aatctccatc atacctttga aaaaggaacg atcaacgaaa atcatgtcct gaaaggcatt
    10081 aatctttcct tagatcccgg cgacttcgtg acgatcatcg gtggaaatgg ggctggtaaa
    10141 tctactttgc tgaacagcat cgccggtaca ttttctgtcg atcagggtca aattttattg
    10201 aatggaaaaa atatcacgaa aaaatctgtt gtcgaacgtt cgaaatccat cagtcgcgta
    10261 ttccaagatc ctaaactagg gacagccgtt cgtttaacgg tagaagaaaa tcttgctctt
    10321 gctatgaaac gaggaaaaaa acgcggcttc ttccgtggtg tcaaaccaca agaccgaaca
    10381 tttttcaaag accagctggt tcggctaaat ttggggttgg aaaatcgttt atcctcagaa
    10441 atcggtttgc tgtctggagg acaacgtcaa gctattacgt tattgatggc cacattacag
    10501 cgtccagaac tgatcttatt agatgaacac actgcagcgc ttgaccctgc tacttccaaa
    10561 acggtgatgg aattgacaga aaagctgatc actgagcaga atctcactgc ttttatggtc
    10621 actcatgaca tggaagacgc gatccgctat gggagtcgac tgatcatgct gcatcaagga
    10681 aggatcgtcg tagatatcca gcaagaggaa aaacagcagc tcacagtgcc ccaattatta
    10741 gcgttgttca aacaaaacag cggcacggca ttaaaagacg atcaagtact tttatcttca
    10801 taatcagaaa gaggatggga caaaaaatga acgcccaccc tcttcttttt tctctttcct
    10861 tctttcgttt ttgctttatg tatcaaccgt tgtttcttcg aaaactattt tgttctctcc
    10921 ttcttcctga tagaatggat gggaaaggag cttgatgcca tgacaacgaa agacaaggta
    10981 cttcaattat tgaaaaagac accagatttt ctttccggag aaaagttggc acagcaactg
    11041 gaagtttccc ggacaagtat ttggaaagcc atcaaagaat tggaaaaggc aggctatcgt
    11101 tttgagcacg ggcctacagg gtatcgttac ctgccctctg acgtactaga tgcaaaagaa
    11161 atccagcaag agctacggtc gtttattcct gatttgaaag tacaaacgat gttgacttct
    11221 gaatctacga tgaaagatgc caaactggca gcagctcaag gagattctgt ttcgaagtta
    11281 tttgttgctg atacacaaga acaagctcgt ggacgttttg gacggccttt tttcgctgaa
    11341 cctggacgag gaatctacat gagtctcttg ctccatccag acaaacggtt tgatgaattg
    11401 cctcaatata cgatcatcgc agccagtgcc tgtgtgaaag ccattgaatc tgtaaccggt
    11461 cgttctgctt cgatcaaatg ggtcaatgat atctacatgg atggaaaaaa agtctgtggg
    11521 attttatctg aagcagtcag tgatatggaa agcggcagga tcagtcatat catcattggg
    11581 atcgggctta atttttctgt tcctcaaaat cattttccag ctgatctcca agaaaaagcc
    11641 atctctgtct ttcccgacgg acaagctagt gtcacaagga atcagctaat cagcaaaatc
    11701 tggcaagaat tgttcactta tctttctcac ccagaaaaag cgttggactt ctataaagaa
    11761 aaatcattcg tattgggaaa aaccgtgtcg ttcaatcaaa aaggaacgac ttatgtcggt
    11821 cttgcaaaag aaatcggtga tcatggcgaa ctgatcgtcc aaacaaatga acagaagatg
    11881 agtttgtctt ctggcgaaat cagtctcaca tctatccgta cgcaataatt atcagatttc
    11941 acaaaaggac acctgatctc atacagtgtc cttttttctg aattctcttc aaaagactct
    12001 ccttattcta tcagctgaca gtttctctat cctctagaaa aaggtattct aaaaatatct
    12061 ataaagcatg gtacactttc tttaaatgaa tcaatgcaaa aaacaagaga gtcatcagtt
    12121 tacacgatca aaagagatag caggccgctt cctttttcaa tcttatagat agaccatggg
    12181 taacgataaa aacaaaaaag aacttgcgac ttattttgcc gtaagttctt ttttagctag
    12241 tgccttaatc tcttaatccc aacaaacgtc tcgcatagat cacctgacca gagtgcatag
    12301 cagcatcatc aataatagaa acgagacgca ttcctcgtgt gactggtggt gtccagttct
    12361 cgtcaattac ttcatctagc tcggaaggtt ccagccttct taagtaagaa atagcaaaat
    12421 cagcagctcc tcgcaagtat cctagcagat cttctatttg attagcttgg atctgctttg
    12481 cttgttccag actatgcttc caatcttgtt cgttatctgc tatcgtcatc ggaaaccggc
    12541 tttcccagtc ttgactatgc caaattggag aagttcctgc taaatcagca atctggaaat
    12601 ctaattctcg agcggtatgc caagccagcc aagtcattga tttgatctgc ggggctctat
    12661 cttccactgg aaaacgattg gcctcctcaa tagagacgcc ttcaaacaaa cgttcaaacc
    12721 gatcgtatgc ccgatccaca ctctcgcata gcatatctaa ataatccatt tattttcctc
    12781 ctttttattt atcctactta ctttttccta attctaggat acctacttat atgttactac
    12841 acacccttat tttttacact ttaacagtca ccatcatcaa tagtttggag attgaaagaa
    12901 aaagatagtg tcgaactatc aattgaacaa gaagcaacaa tatgaacgtt tcattaatat
    12961 tctttaaata aacaatgatt tttcaaacga acagtttaag actaggagaa aaagagtata
    13021 ataggaaaat gtttgaaagg aaaggaaggg gaaaaatgaa aaaaagggca agtttactag
    13081 tcctagttgc tttgttgttc aatatctttt cgtatggtgt ggccgtttca gcggaaacat
    13141 acattcctaa agaccctggg aatatttata atgatctaac tggtaactat ggcttgttag
    13201 gaattgctag tcaattccac gtatttgcta aaaataagac aactgtgaat gcacatacag
    13261 acggaaatat agcaacacat gaacttgatg caaaaaataa ttttggtaca gagcttcatg
    13321 aaggtttatt gaaccaagaa ataaattata tacaaaaaac tgattctttc gctaattctt
    13381 caggcatacc tacaggtagc acaagaatga ataaatttgt cgttggcagt aacgtggatg
    13441 tagcacttca agatggtcat cctacagtca atggatccag gcttgatcac ttaactccag
    13501 atgattttta taaagatcgt gctgggcatg aatatattga tttcgagcag gaatttgcta
    13561 aattgaatgt agcagcaaat gatcttgcaa cgatcactcc agctaaaaca tatactgctg
    13621 cagattttcc agacatgaac aatcggacaa tcgatttaac tgatttatca gataccgggt
    13681 ttttatt
//
LOCUS       DAXWOO010000068        13674 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.068, whole genome shotgun sequence.
ACCESSION   DAXWOO010000068 DAXWOO010000000
VERSION     DAXWOO010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13674
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.068"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            50..292
                     /gene="yaaA"
                     /locus_tag="ACGE70_001543"
     CDS             50..292
                     /gene="yaaA"
                     /locus_tag="ACGE70_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFF8693185.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            279..1403
                     /gene="recF"
                     /locus_tag="ACGE70_001544"
     CDS             279..1403
                     /gene="recF"
                     /locus_tag="ACGE70_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFF8693186.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1400..3346
                     /gene="gyrB"
                     /locus_tag="ACGE70_001545"
     CDS             1400..3346
                     /gene="gyrB"
                     /locus_tag="ACGE70_001545"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFF8693187.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3366..5837
                     /gene="gyrA"
                     /locus_tag="ACGE70_001546"
     CDS             3366..5837
                     /gene="gyrA"
                     /locus_tag="ACGE70_001546"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFF8693188.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6073..6372
                     /gene="rpsF"
                     /locus_tag="ACGE70_001547"
     CDS             6073..6372
                     /gene="rpsF"
                     /locus_tag="ACGE70_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFF8693189.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6422..6934
                     /gene="ssb"
                     /locus_tag="ACGE70_001548"
     CDS             6422..6934
                     /gene="ssb"
                     /locus_tag="ACGE70_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFF8693190.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            6960..7196
                     /gene="rpsR"
                     /locus_tag="ACGE70_001549"
     CDS             6960..7196
                     /gene="rpsR"
                     /locus_tag="ACGE70_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFF8693191.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7339..9312
                     /locus_tag="ACGE70_001550"
     CDS             7339..9312
                     /locus_tag="ACGE70_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFF8693192.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9318..9770
                     /gene="rplI"
                     /locus_tag="ACGE70_001551"
     CDS             9318..9770
                     /gene="rplI"
                     /locus_tag="ACGE70_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFF8693193.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            10044..11411
                     /gene="dnaB"
                     /locus_tag="ACGE70_001552"
     CDS             10044..11411
                     /gene="dnaB"
                     /locus_tag="ACGE70_001552"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFF8693194.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            12191..13483
                     /locus_tag="ACGE70_001553"
     CDS             12191..13483
                     /locus_tag="ACGE70_001553"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFF8693195.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT         4553 a         2474 c         3013 g         3634 t
ORIGIN      
        1 aaaaggtata atagagtata cttttaataa tgaaaatgag ggatgggcat tgaaacaaca
       61 agtcattcta aatacggaat tcatgacgtt gggccaaatg ttgaaagaag tcacagttat
      121 cggtagtggc ggacaagcaa aatggtattt ggcagaaaac actgtgttag tcgatggcga
      181 accagaaaat cgtcgtggac gcaaacttta tcccggcatg atggttgaag ttccagaagt
      241 aggaactttt tttatggtaa aaaaaggaaa cgacgataat gaggctgaat aagctgtatt
      301 taaaaaatta tcgaaattac gaagagctga acatcgaatt ttccaagaac ctcgtcatct
      361 ttttaggaga aaatgcgcaa ggaaaaacga atcttttgga aagtatctac gtattagcta
      421 tgacgcgaag tcatcggaca agtagtgagc aggaattgat tggctggact gacgatcagg
      481 cgatgattca aggagagatc acaaaaggtt catccactct tccattagaa atcttattat
      541 ctaaaaaagg ccgaaaaacc aagatcaacc atatcgaaca gaaaaagtta agtacttacg
      601 tgggacaaat gaatgtcatt ttatttgcac cagaagatct ttctttagta aaaggcagcc
      661 cgcagaccag gcggaagttt ttagatatgg aattaggcca gatcaatcca atctatcttt
      721 atgatcttgt ccaataccaa gcaattttga aacaaagaaa ccagtacttg aagcaattga
      781 atgaaaaaaa acaaactgat ttgctttatt tggatatttt gtccgaacaa ctagcagcgt
      841 ttggcagcaa agtattgaaa gcaagagcac agttcgtcaa gcgattggag ttttgggcta
      901 attctttgca tcagcaaatc acccatcaaa aagagcagct ggaaatcgag tatctcaccg
      961 cagtcgattc tttagaaaca catacgcaag agcaaatcca agaacaattt ctcgcacttt
     1021 tgaaccaaaa taaaaagaaa gacctttttc ggggaacaac gacagttggt cctcatcgtg
     1081 acgatctttc cttttttatt aatcagaaaa atgtgcagac ttatggatcg caaggacagc
     1141 aaagaacaac tgcactaagt gtcaaattag cagaaatcga tttgattaaa gaagagacag
     1201 gggaatatcc aatccttttg ttagatgatg tgatgagcga gctggatgat aatcgtcaac
     1261 tccatctgct agaaacaatc gaaggaaaag ttcaaacgtt cttaacaaca acgacacttg
     1321 accatgtaaa aaataaaatg actgttgaac ctgagatttt ttatgttcaa caagggaaac
     1381 taaaaggaga agaaactgaa tgacagaaga aagaagttta gtagaacgcg ctaaagagta
     1441 tgatgccagt cagattcaag tacttgaagg acttgaagcc gttcgtaaac gacctggcat
     1501 gtatatcggt tcgactagtt ctgagggtct gcatcacctt gtttgggaaa ttgtcgataa
     1561 ttcgatcgat gaagtattag caggttttgc aacgaaaatc catgtgatta ttgaaaaaga
     1621 caacagtatc accgttattg acgacggtcg ggggatacct gtagatatcc aggcaaaaac
     1681 tggacgtcct gcagttgaaa cggtctttac tgttttacat gctggaggaa aattcggcgg
     1741 tggtgggtac aaagtttctg gtggacttca cggagtagga tcttctgttg ttaacgcgct
     1801 ttctacacag cttgatgtca aagtatacaa agaaggaaat gtgtactatc aagaatatcg
     1861 tcgtggagct gtagtggacg acttaaagat tatcgaacaa acggatcgcc atggaacaac
     1921 ggttcacttt acgccagacc ctgaaatttt tacagagaca actgagtttg atttttcaaa
     1981 attggctacc cggatccgtg aacttgcttt cttgaaccgc gggatgcgta tatcgattga
     2041 agacaaacga gaagaagaac cagtcatcaa tgaataccat tatgacggcg ggattaaaag
     2101 ttatgtcgaa tacttgaatg caaacaaaac cgtgattttt ccagaacctg tatatttgga
     2161 aggtgaacag caagatatcg cagttgaagt ctctatgcaa tatacagacg gctaccattc
     2221 caatatcatg agttttgcca acaatatcca tacgtatgaa ggcggaacac atgaatcagg
     2281 attcaagact gctttaactc gtgtgataaa tgattatgcc cgaaaacaaa agctgatgaa
     2341 agaaaatgac gacaacttga ccggtgaaga cgtgcgagaa ggattgacag ctgttatttc
     2401 tatcaagcat cctgaccctc aattcgaagg acaaacaaaa acaaaattgg gcaattccga
     2461 agtacggaca gtcactgatc ggttatttag cgaacatttc atgaaattct tgctggaaaa
     2521 cccaagtgtg ggccgtcaaa ttgttgaaaa aggattacta gcttccagag ctcgtctagc
     2581 agcgaaacgt gcgcgtgaag taacgagacg taaaggtgct ttagagatta gtaatctgcc
     2641 aggaaaatta gcggactgct ctagcaacga tcctgaaaaa tgcgaattgt tcatcgtcga
     2701 aggagattcg gccggcggat cagctaagca aggtcgtaat cgagagtttc aagccatctt
     2761 gcctattcgt gggaaaatct tgaatgttga aaaagcaagc atggacaaga tcttggcaaa
     2821 tgaagagatc cggtctttat ttactgctat gggtacaggg tttggagaag attttgatgt
     2881 atcgaaagct cgctaccaca aattagttat catgactgat gccgatgtcg acggcgcgca
     2941 tatccgtaca ttgctgctga ctctttttta tcgttacatg cgtcctattg ttgaagctgg
     3001 atacgtctat attgctcagc cacctttata cggggtaaaa caagggaaga atattacgta
     3061 tgtacaacca ggaaaaaatg cagaagaaga gttgaaacaa gtcgttgctt cacttcctgc
     3121 ctcaccaaaa ccaagcgttc agcgttataa aggacttgga gaaatggatg atcatcaact
     3181 atgggaaaca actatggacc caagcaatcg gatgatggcc cgcgtcagtg tggacgatgc
     3241 tattgcagca gatcagatat ttgaaatgtt gatgggggat cgagtagaac cccgtcgtgc
     3301 attcatcgag gaaaatgcac actatgtgaa gaacttagat atttaatcat gaaggaggaa
     3361 aaagaatgag tgaagaaatc agagaaaaca tccatgatgt caatctaacc agcgaaatga
     3421 aagactcctt catcgattat gcgatgagtg ttattgtcgc acgtgcactt cctgatgtca
     3481 gagatggtct gaaacccgtt catcgccgta ttttatacgg catgaacgaa ttaggtgtaa
     3541 caccggataa agcccataaa aaatctgcca gaatcgtcgg agatgtcatg ggtaaatacc
     3601 atcctcatgg agactatgcg atctatgagt caatggtacg gatggcacag ccattcagct
     3661 accgttatat gctagtggat ggtcatggaa actttggttc tgttgacggt gacggtgccg
     3721 ccgctatgcg ttataccgaa gcacggatga gcaaaatcgc tacagaaatg ctgcgtgata
     3781 tcaataagaa cactgttgat ttccaaagca actatgatga tacagaaaaa gaaccagttg
     3841 tactacctgc tcgcttcccg aatcttttag tgaacggaac aacggggatc gccgtaggta
     3901 tggccacgaa tatcccacct cataacttgt ctgaggtagt agcggctatc gatttgctga
     3961 tggataatcc tgatgtgacg accaatgaat tgatggaagt attacctgga ccagattttc
     4021 caacaggtgg tttggttatg ggaaaatcag gaatccgaag agcgtatgaa acaggtaagg
     4081 gttctattac cgtacgtgca aaagttgaga ttactgagat gccaaatggt aaagaacgga
     4141 tcatcgtgac agaattgcct tatatggtca ataaagcaaa attgatcgaa cggatttctg
     4201 aactgcacag agacaagcgt atcgaaggga tcacagattt gcgggacgaa tcttcccgtg
     4261 aaggtatgcg gatcgtcatt gacatccgtc gtgacgccag cgcatctgtt atcttgaata
     4321 acttatataa attgacatcc ttgcaaaatt catttggatt caatatgttg gcgatcgaaa
     4381 aaggtgtgcc gaaagttttg agcttgaaac aaattcttga aaactatatc gaacatcagc
     4441 gcgaagtcat cacaagacgg acagtctttg aaaaagaaaa agcagaagca agggcacaca
     4501 ttttagaagg tttgcgtatt gctttggatc atatcgatga aatcattgcg attatccgcg
     4561 ggtctaaatc agatgacgaa gcaaaagcga cgatgatcga acggttcgat ttatccgatc
     4621 gccagtctca agctatttta gatatgcgtt tacgccgatt gaccggtttg gaacgggaaa
     4681 aaatcgaaaa tgaataccaa gaactcttaa aactgattga agatttaact gacattctgg
     4741 caagaccaga acgtgttaca gaaatcatca agaatgaatt agcagacttg aatcaacgat
     4801 ttggtgataa acgacgcaca gaattactag tcggtgaggt cttaagtctg gaagatgaag
     4861 atttaatcga agaagaagaa atcgttatta cattgacaaa caatggttac atcaaacgtt
     4921 tggcaaataa tgaattcaga gctcaacgaa ggggtggacg tggtgtccaa ggaatgggta
     4981 tccacaatga cgacttcgtc aaaaatctgg tttcttgttc tacccatgat acattgttat
     5041 tctttacgaa taacggaaaa gtgtatcggg caaaaggata cgaaattcca gaatacgggc
     5101 gcacagccaa aggaatacca attatcaatt tactaggtat cgattcgagt gaaagcatcc
     5161 aggcaatcat tgcggtgacg ggagaagcag aagaaggcca ctatctcttc tttacgacgc
     5221 gtcaaggtac agtaaaacgt acttctgtca ctgcttttgc taacatccga agtaatggtc
     5281 tgatcgcaat tggtttgaaa gaagacgatg agctcgtcaa tgtgctactg acagatggtc
     5341 aatctaatat cattattgga acacataacg gctactctgt cactttcaaa gaagaagcag
     5401 tccgcgatat gggacgtaca gcttccgggg ttagaggaat acgcctgcgt gaggaagatt
     5461 atgtcgttgg ggcagcctta atgaaggaag atcaagaagt attagtgatc acagaaaaag
     5521 gttacggaaa acggacaaaa gtatcggaat atccagtgaa aggccgcgga ggtaaaggga
     5581 tcaaaacggc gaacatcact gaaaaaaatg gtccactagc tggattgacc actgtttctg
     5641 gagatgaaga catcatgctg attacagata aaggtgtcat tatccgtttc aacgtatcga
     5701 ctgtttctca aactggacgt tcaacattag gcgtacgttt gatgaagatg gaagctgata
     5761 cgaaagttgt cacgatggca gcagtcgaac cagaagctgc agaacaaaca gttgaagaac
     5821 aaggatcaga agaatagcca tcaagactaa ttgtgtaagc tagcaagaat gaccgattaa
     5881 aaaatctggt tattcctgct ggcttttttg attagttgaa tttctcttaa aatattcgaa
     5941 actgttcatt ctgtattgct tttgaccaaa atagagtgta taatcagagc ttgtgagtag
     6001 tctaattaat tagataactc tccttgctct taggaaccta agagccgaaa gtccacaggg
     6061 aggtgaatat caatggaaaa cacgaaatat gaaatcatgt acattattcg tccaaacatt
     6121 gatgaagaag caaaaactgc tttaatcgaa cgtttcgatg caatcttgaa agataacgga
     6181 gcagaagtta tcgaatcaaa agattgggaa aaacgccgtt tcgcttatga aatgaacggt
     6241 taccgtgaag gtatctatca catcgttaaa gtatcttctc catcatcagc aaatgctgtc
     6301 aatgaatttg accgtcttgc taaaatcaac gacgatatca ttcgtcacat gattgttaaa
     6361 gaagaagctt aattgttgtt tcatgtgaaa caatagaaaa cgatgagagg agatgactat
     6421 tttgattaat aatgttgtat tagttggacg tctgacgaaa gatccagatt tgcgttacac
     6481 agcaagtggt acggcagtcg caacatttac tttggctgtg aatcgtaatt ttacaaacca
     6541 aagcggcaat cgcgaggctg attttatcaa ctgtgtgatt tggcgcaaat cagcagaaac
     6601 tttagcaaac tatgctcgta aaggaacact tttaggtgtt actggacgta tccaaactcg
     6661 ttcttatgat aatcagcaag gacaacgtgt atacgtgaca gaagtagtag ctgacaactt
     6721 ccaattgctg gaaagtcgtt ctgcatctga aaatcgccaa caaagcggcg gattccaaag
     6781 ttctggtcaa tcagctggcg gatttggcgg aaacaataac tctaatcaaa catctcaatc
     6841 atccaacggt atgcctgatt tcgaccgtga tacttctgac ccattcggct caagttcaac
     6901 gatcgacatt tcagacgatg atttaccatt ctaaacgaat ttgataggag ggaaatagaa
     6961 tggcacaaca aagaagaggc ggacgtaaac gtcgtaaagt agactacatt gctgctaacc
     7021 atattgaata tatcgattat aaagatatcg aattactaaa aagatttatt tcagaacgcg
     7081 gtaaaatctt gccacgtcgt gtaactggta caggcgctaa aaaccaacgt aaaattactg
     7141 ttgcaatcaa acgcgctcgt atcatgggct tgctaccttt cgttagcgaa gactaataaa
     7201 tcctaggaat tgaatgactg atccttttga tcagctattc aattcctttt tttctgtaaa
     7261 aataacctct attcgtttag tcgctcgaat gacgaaagtt atggtaaaat caaattagtc
     7321 atatatggga ggaaaattat ggaaaataaa aacaatcatt tatccccgaa acctctcttc
     7381 cttatcctgt ttatattaat cgaagtattg gtagtcttaa ttttaccatt tcgttttgtt
     7441 agcgcttcgg tcattctatt gatgaattta tggggtgttc gctattggtt gaaaatgaaa
     7501 cgttggctta ttttaaataa acgtgaaaaa atccagcagg cttcgactgt agctgaaacc
     7561 aatctagctt atgtctcaga agtcatgcct gtaggagtga ttgcttatca gccatcaagc
     7621 caaaaaatcg aatggctcaa tccattagca ttgaaaatca aagatcaaag tggattaaca
     7681 gaggaagaat tgctagatac tttttttact gcaaaagaaa aaaagaaaaa ccaagtctat
     7741 ctagcagata aaacatttca gtttgatatg gatacagtaa agggtgttgt ttattttaat
     7801 gatattaccc aaaaccagtt gttaaaactc catcagacga acagtcagcc ggtgattggt
     7861 attttatcta ttgataacta tgcagatgcc attgataaat tggatgacaa agagatttct
     7921 tatttgaact ctttcgtcac tacactagtt tctgactgga tgaatgaata ccatgtcttt
     7981 ttcaaaagaa tcaacgcaga acggtttttc ttcgtagcac agatgatcga tctgaaaaag
     8041 atgatggata aaaaatttga tttgctggat cgctttcgag cagaagctga aaaacagagc
     8101 tcgcctatca cccttagtat ggggattgct tatggctcaa gaaccctcga tgaaatcggt
     8161 ggagaagctc agaataattt agatgttgct ttagtccgtg gaggcgacca agttgtcgtg
     8221 aaagaggtaa aagaaggagc aaaacctgtt tattatgggg ggaaatctgc taagaatgtc
     8281 aaacgcacgc gtgttcgttc tcgagcgatg agtaccgctt tgcgtcggat ttttgatgaa
     8341 acaggcgatg tgtttatcat ggggcacaag tttccggata tggatgcttt aggctctgct
     8401 tttggtgtca gttgtctggc acgcttcaac cagaaaaaag cttacatcgt tatcaacgag
     8461 aacgagatca ttccagatgt agaacggtgt cttgaagaaa tccataagca tcctgaatta
     8521 gaaatgcaac taatctctcc agaacgtgcc atagagctga aaaaagaaga ggacctcctc
     8581 gtgatggtgg attaccataa accttctcta tcgatctctc agggcctata tgagcaattt
     8641 gataagattg tgatcattga tcaccatcgt cgtggggaag agtttccaag taaacctctg
     8701 ctgacttata tcgaatcttc agcttcttca gcttcagagc tagtttctga gctgatccaa
     8761 taccaaagca gcaaaacaaa tcgcttagat cgttttgagg ccacattatt gcttgcaggg
     8821 atggttgtag atacaaatag tttcaatgtc cgtacaactg cacgaacttt tgacgttgcc
     8881 agctatttaa ggtcttgtgg agcagacgca tccttagtac aatatttatt gagctcggat
     8941 ttagcgacat acttggagat cagcaatttg attgctggaa gcgactatgt gacttctgat
     9001 attgttgtgg cggcagggac agacgaaaaa gagtataata gtgttatagc agcaaaaaca
     9061 gcagatacat tgctttcgat gatcaatatc aatgcagctt tcgtcattac aaagcggaca
     9121 gacggcttga ttgggatcag tgccagaagt accggcacga tcaacgtcca aatcatcatg
     9181 gaatccttag gtggcggagg acacttcaca aacgcagcta ctcaactgga aaatacgaca
     9241 gttgaggaaa caaaacagaa gctatttgct gcaatcaaag aaaatatgaa tcaaatatat
     9301 gataaggagt aatccaaatg aaagtaattt ttttacaaga tgtaaaagga aaaggtaaaa
     9361 aaggggacat caaagaagtt ccagcaggtt atgcacaaaa ttatctaatc aaaaataact
     9421 tggcaaaaga agcaacgaaa agcagtatca gtcagttagc aggacaaaag aaagcacaag
     9481 aaaaacacga agctgaaatc ttggaagaag ctaaaaagtt aaaagaattc ttggaaaaag
     9541 aagatactgt agtcgaattg aaggcgaaag caggggaaga cagccgtttg tttggttcga
     9601 tcccttcaaa acaaattgct gaagggttaa acaaacaata caaaatcaaa ttagataaac
     9661 gaaaaatcga attagaaaat ccgatccgtt cactaggcta cacaaacgta cctgttaagc
     9721 ttcatcatga ggtgacggct acgatcaaag ttcatgtcgt acaagaataa caaatcttga
     9781 tagcgatgtt cagctggtaa gtataaccag aaggaaaaaa gacaaaccga aatttatctg
     9841 agaaaacagc tgcagcaatg aaagcaaaag ctgcaaatcg atttcagatt gatttcggtt
     9901 tcttttttgg gaacagcaaa atagcataaa gaaaacgagt ataataaaag agaactattt
     9961 ttttattaca ctttcggtac gaatgatgac ttttctctca ttttcttgta aaatgaagca
    10021 attgaagaca aggagcgata cgtatgaatg aagtgtggca ggatcgaatc ccaccgcaaa
    10081 atattgaagc tgaacaagcg gttttaggct ctgtttttct tgacgcagaa acgatcattg
    10141 atgccatgga gtacatagag ccaaaagact tttatcgtcg tggccatcaa attatttttg
    10201 agacaatgat cgagttaaat gatcgtaatg aagcgattga cgtggttact gtcaaagacc
    10261 ggctggaaca agccaatttg ttagaagatg ccggtggttt aagttatctc tcagacttgg
    10321 cattagcagt tccgacagca gcaaatatcg tctactatgc gaaaatcgtt gaagaaaaat
    10381 cattgctgcg tacattgatc cagacagcaa caggaatcgt tacgaaagga ttcgagcaag
    10441 gagaagaagt tcaaacaatt ttagatgatg ccgaacgcag tatcttggag gtttcggaaa
    10501 aaagaaatcg aagcggcttt ttatctattg ctgaagtact gaactccacg attgaaaata
    10561 tcgatcgact agctcaaaac aatgaagaaa tcacaggtct accaactggc tatcaaggtc
    10621 tggataaaat gacagcaggt ttccagcccg aagaactaat cattcttgct gcacgtccag
    10681 cggtaggtaa aacagcgttt gcattgaata ttgctcaaaa tgtaggaaca aaaacagatc
    10741 gttctgttgc gatatttagt ttggaaatgg gggcagaatc tcttgtaaat cggatgcttt
    10801 gtgcagaagg ttcgattgag gccagccatt tacgtacagg tcagctttcg gaagaagaat
    10861 ggtcgaatct gatcgttgcg atggggagtc tttctcgagc aaatattttt atcgatgata
    10921 caccagggat caaaatctct gaaatccgtg cgaaatgtcg gaaactagcg caagaaaaag
    10981 gcaatctagg cttgatattg atcgattatt tgcagttgat tgaaggtaac ggtaaagaga
    11041 accgtcaaca agaagtatca gatatctctc gccaactgaa aaaactggcg aaagagctga
    11101 aagtacctgt cattgctctt tcacagttat ctcgtggagt agaacagcga caagacaaac
    11161 gaccagtgct aagtgatatt cgtgaatccg gatcaattga acaagatgcg gacatcgtag
    11221 ccttcttgta tcgagatgat tattatgaaa gagaaggcgg agatgacgat gatggaaacg
    11281 agccaaaaaa tgacaacgtc atcgaagtca tcatcgagaa gaaccgaagc ggtgcacgag
    11341 gaacagttga actactgttt atcaaagaat acaataagtt ttcgtcgctt tcgccaagag
    11401 aagaatttta aagaagaggt tgtgagcctt gagcaatccc gagagctctc gtaagaagat
    11461 gcaaggagta accgcaggaa cgactagagt acttaattta aacagatctc caaagggatt
    11521 gtgacaaaag tcttgtcaca gtcccttttt ccgaataaac ggtgttcaat cagctgaccc
    11581 ttggtattag ctcagcaaac gtcaaaatat gaggaactat tttgacgttt gtttctctaa
    11641 tactcaggtc aaaacgctga tcttacgacc tcttttcctt aaacggtgtt ctcctgccaa
    11701 ctcctcggtc acaagtcaca atatcatcaa acatgagaag cagttttttg aaattgctcc
    11761 ttgtgactca gagttaaacg gcaggctcac aacctctttc ttaaacggtg ttcaaaagct
    11821 tgcatctgcg gtaatacgcc caatcaaacg aaaaatatga gaagctattt ttgtttggct
    11881 gttcttatta cttgatgcag gacagctttt tcacaacctc tgattcacgg tgttccaaaa
    11941 gtagcttctt cgaaaaataa gtcgaaattt cacaaaaatt tgagaagcaa ttttcgtaca
    12001 ttccttctta tttcttgaag ctgaccactt ctgttacaac ctctttttta ataaccattc
    12061 taaaaagata aagttcgttt tttaagcgtt tttataaaat aaaaataaaa atcattcgat
    12121 tttatttgaa atatgtatct attattgata caatggttca ggattaaata atatagaacg
    12181 aggtgttcac atgtcatctg tagtagtagt aggtacgcaa tggggcgatg aagggaaagg
    12241 aaaaatcaca gactttctca gtgaaaatgc tgaagtgatt gcacggtacc aaggtggcga
    12301 caatgcaggg cacacaatta agtttaacgg tgtgacttat aaactgcatt tgattccttc
    12361 tgggattttt tataaagaca agatcagtgt gatcggaaac ggcgttgtag tcaatcctaa
    12421 atcattggtt aaagaattag cttatttaca tgaaaaaggt gtagaaacaa gtaatttgcg
    12481 tatttctgac cgcgcgcacg taatcttgcc ttatcatatc aaattggacc aattacaaga
    12541 agatgctaag ggcgaaaata agattggtac aacgattaaa ggaatcggac ctgcctatat
    12601 ggataaagca gctcgtgtag gtatccgtat tgcagactta ttagataaag aaatttttga
    12661 agaacgtctt cgtttgaatc tagatgacaa aaaccgtcaa tttgtaaaaa tgtttgattc
    12721 agaaccactt gattttgacg atatttttga agaatattat gaatacggac aacaaatcaa
    12781 acaatacgtc acagatactt ccgttatctt gaatgatgct ttggatgcag gcaaacgcgt
    12841 gttgtttgaa ggcgcacaag gtgtaatgct ggatatcgac cagggaactt atccttttgt
    12901 tacttcttct aacccagtag ccggtggggt aacaatcgga agcggtgtag ggccttcaaa
    12961 aatcaataaa gttgtcggcg tctgcaaagc ctatacgtca cgtgtcggtg atggaccatt
    13021 tccaactgaa ttatttgatg aaacaggaga acaaatccgc gaagtaggac gagaatacgg
    13081 tacgacaact ggtcgtccgc gtcgtgtagg ctggtttgat tctgtagtta tgcgccattc
    13141 taaacgagtt tcaggtatta ccaacctatc tttaaactcg atcgatgtat taagtggatt
    13201 ggaaacagta aaaatttgta cggcttatga attagatggc gaactaatct accactatcc
    13261 agcaagcttg aaagagttga accgctgcaa accaatctat gaagaattgc caggctggtc
    13321 agaagacatt acaggatgca aaacacttgc agaactgcca gaaaacgcac gtaactatgt
    13381 acgtcgtatc tcagagcttg ttggtgtacg tatctcaaca ttttctgtcg gacctgatag
    13441 aacacaaaca aacatcttag aaagcgtctg ggcgcaaatc taaggataaa aacgacgact
    13501 gaatcaggac gttcttacca gagcgagtcg aagtgtttat ctatacgata ataataactt
    13561 gaagaggtag tgaaagagac gtttagcctt attgccgata ggctgtctct tgaacactgt
    13621 ttattcagaa taagggactg tgacaaaaac cgtgtcacag tccctttttc cgaa
//
LOCUS       DAXWOO010000069        13527 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.069, whole genome shotgun sequence.
ACCESSION   DAXWOO010000069 DAXWOO010000000
VERSION     DAXWOO010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.069"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            115..1854
                     /gene="yfcC"
                     /locus_tag="ACGE70_001554"
     CDS             115..1854
                     /gene="yfcC"
                     /locus_tag="ACGE70_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFF8693196.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2011..3195
                     /gene="iadA"
                     /locus_tag="ACGE70_001555"
     CDS             2011..3195
                     /gene="iadA"
                     /locus_tag="ACGE70_001555"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFF8693197.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3306..3509
                     /locus_tag="ACGE70_001556"
     CDS             3306..3509
                     /locus_tag="ACGE70_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFF8693198.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3610..4272)
                     /locus_tag="ACGE70_001557"
     CDS             complement(3610..4272)
                     /locus_tag="ACGE70_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8693199.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4285..5787)
                     /locus_tag="ACGE70_001558"
     CDS             complement(4285..5787)
                     /locus_tag="ACGE70_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8693200.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6335..8014
                     /locus_tag="ACGE70_001559"
     CDS             6335..8014
                     /locus_tag="ACGE70_001559"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFF8693201.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8141..8620
                     /locus_tag="ACGE70_001560"
     CDS             8141..8620
                     /locus_tag="ACGE70_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFF8693202.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8839..9519
                     /locus_tag="ACGE70_001561"
     CDS             8839..9519
                     /locus_tag="ACGE70_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFF8693203.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9527..10858
                     /locus_tag="ACGE70_001562"
     CDS             9527..10858
                     /locus_tag="ACGE70_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFF8693204.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10873..11835
                     /locus_tag="ACGE70_001563"
     CDS             10873..11835
                     /locus_tag="ACGE70_001563"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFF8693205.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11946..12284
                     /locus_tag="ACGE70_001564"
     CDS             11946..12284
                     /locus_tag="ACGE70_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFF8693206.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12363..13070
                     /locus_tag="ACGE70_001565"
     CDS             12363..13070
                     /locus_tag="ACGE70_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFF8693207.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(13173..>13527)
                     /locus_tag="ACGE70_001566"
     CDS             complement(13173..>13527)
                     /locus_tag="ACGE70_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HFF8693208.1"
                     /translation="NGERNQISQYLSQLIQHLLQTETKEQLLIHLQITKQFTGNYHLQ
                     SHLQQLFRKDAVKITAYSEHADLIITDQHEKISDETETFYLDTTHNTVLFGKLIKAVH
                     ELYMKKGYENKTKRM"
BASE COUNT         4275 a         2385 c         2952 g         3915 t
ORIGIN      
        1 aagtgatttt ctaataaatt attcatgtgg aaatgtcatt tttactgtat aattcatttg
       61 attatccatt ttttattcat caaaattact gaaaaaagaa aggaagaaat aaatttgaag
      121 aaaacaaatt cttcaggaaa accgaaacgc gacttcacta aattatcgac accccatacg
      181 tatgtcatta tttttggtgt agtcatcttt gcatggatac tgacttttgt cgttccagca
      241 ggaaagttca gcacgcaaga tattgaatac aaagatgcaa atggcgaaac aagtactcgt
      301 actgttttac gtcaagattc cttccgttat gcttacgagc tggataagtc gtatgtgttt
      361 gatcaattag aagaactgca agatcatcca gcagaacgtg aaaaactgga tgttccggaa
      421 aaaggattgg aaaaagtcat tgctgacgga gaaaaaaatc ttacgcagga aaaactagat
      481 gaaatctcct tgacagatga tgtgctttac gatcagtacg gcgaaaatat ctatgatact
      541 tctaaaaaat tgcataaaac agccaaaata tggggaacag atgattttgg cggctttggt
      601 ttcttaaatt ttgtttttga aggactagta tctggagata agtatggatc tgctgtaggg
      661 atcgctgcat tgatccttgt tgttggaggt gcttttggta ttattatgcg taccggagcg
      721 atcgacgcag gtatctatgc gtttatcagt aaaactaagg gactggagcg tttagcttta
      781 ccgctattat tctttgcttt ttcattcggt ggcgctacat tcgggatggc agaagaggta
      841 attccattct caatggtgat ggttccattt gtcattgcgt taggatatga ctcgatcgta
      901 gcagtaaccg tgacttatgt cgcatctcaa gtcggaaatg cgacctcttg gatgagcccg
      961 tttagcgtag cggtggctca agggattgct ggaatccccg tgttgtctgg tgcgacattc
     1021 cgattgatta tgtgggtagt tgttactgca ttagccgctg gttatatgat gatatatgca
     1081 gaaaaaatcc gtaaaaaacc agaaagttcg ttgacttaca aatcagatga ttatttccgt
     1141 tctcatatca aaaagacatc tgatgaaaat aaaaaattca tgcttgggca taaattgatc
     1201 ttattagaga tgttagttgt ccttgtctgg atcgtttggg gcgtgactca aaaaggctat
     1261 tacattccag aaatcgcttc tcaattcttt gtgatgggat tagttgctgg aatcattgct
     1321 gttcttttca aattagatgg aatgaaagtc aatgacatcg cttcttcttt ccaatctggt
     1381 gcagctgatt tagctggtac agcaatcgta gtaggtatgg ccaaagggat tcttcttgtc
     1441 ttgggaggat ctgatgcatc tgttccttca gctttgaata cgattttgca tgggatcggg
     1501 acggcgttga ccggtgttcc agctgtcatt ggtgcttggg cgatgtacat cttccaaagt
     1561 ttgttcaact tggttgttac atcaaattca ggacaagcag ctttgactat gccaatcatg
     1621 gctccactgg cagatttagt aggtgtatcc agacaagtag cagttcttgc ttatcagctt
     1681 ggttcaggtt ttatggacgc ttttacaccc gtatcagcca gcttgattgg tgtgctaggt
     1741 gttgcacgga tcgaatgggc aaaatgggca agattccaaa tcaagatgca aggattcctg
     1801 tttgttttag gaactatttt catcatgatt gccattgcaa taggtctgca gtagtactgg
     1861 agacctgtaa ataaaaaagt aatccaaaca gtagataagg actccaagaa ccgtcatttt
     1921 acggtaaaat caaaggagta aagatcctat cgtttggatt attttttaag atcctatcat
     1981 cagttatact taaagaaaga ggaaaaatgt atgaaaatca ttcgacaaat tgaagtgttt
     2041 gcaccagatt acttaggaaa aatggatgta ctgattgccg gtgggaagat tttggctgta
     2101 gaagaacagc tagaaggcgg ctacgaagga gtggaagtag aagaactttc aggtgaggga
     2161 aaagtactaa ccccgggttt cattgactgt catttccata ttttaggtgg aggaggagaa
     2221 ggcggttatc aaaatcgaac gccagaagtc acattgagcc aattgactac agctggtgta
     2281 acgacagttg tcggttgttt gggaacagac ggagaaggta gagatatgac tgcgttgatc
     2341 agcaaagcga aaggtctgga agcagaagga atctccactt atgtatacga aggttcgtac
     2401 cgattgccgg taaaaacagt aacagattct atcatcaaag actttttgac aattgataaa
     2461 attatcggaa tcggagaaat cgcagtatcc gatcaccgtt cttctcagcc aagttttgaa
     2521 gagttcgctc gtgcagtagc agatgctcgt gtaggaggca tgttatccgg aaaagctggg
     2581 atcatcaatg tccacttagg cggaggaaaa agaaagctag agctattgac acgaatcgta
     2641 gaagaaacag aaattccgat cacacaattt ttgcctactc atgcaaatcg cacaccagaa
     2701 ttatttgaag cttgcgtggc atttgccaaa cgtggtggca cgattgactt cactgctagc
     2761 gaagatcctg atttttggga gaagacagat ggtgaagttc gcttcagcaa agcactgaaa
     2821 cgattgatcg aggaagatgt ttctttagat aatttcacga tgacctcaga tggacaagga
     2881 agcttgcctt attttgatga aaacaatcat ttcctagggt taggagtagg aagtgccaag
     2941 gcattgctag tagggatcaa agaagctgtg caaaaagaaa gtatcccttt ggaaatcgcc
     3001 ttacgagcta taaccagtaa tccagcacgt atattgaaat tggataaaaa aggaaaaatc
     3061 gaaattggtg ctgatgcaga tctgtgtatc ttggataaag aaacgcttga tattgataca
     3121 gttatcgcta aaggagaaat aatggtccaa gaaaaagaag tcaaagtatg ggggactttc
     3181 gaaaaaagtt tttaatcaac agctctaact atctttttta gaaaacgagc gttctgtttg
     3241 tctgtatctg ttgttgatgt taccctgaag gtagatcaat aaacaaacta agaggaggaa
     3301 gcacaatggc agatttaaaa ggacgttcta aagatatgaa agataaagta gtcggtgaag
     3361 gaaaagaagc atacggcaaa gcaacagacg acaagagcaa agaaacagaa gggaaagcac
     3421 aatcactagg tgctgacatc aaagaaaaag ctcgaaattt aggcgacgaa gtcaaagaca
     3481 agttttctcg aaaagacgaa aaaaaataaa aaccacttat tactaattga atcaagcaaa
     3541 taaaaaatcc gaaccatatc tgcgagacag atatggttcg gattttttat ttgatcatta
     3601 tcctgttttt taaacttcga ttttttgaaa ttttttattt tttagttcaa agacttcatc
     3661 acagcttttt gctacttcaa tctcatgagt cacgatgata atacagcgat tttgttcatg
     3721 ggcgatttct tggaaaagtt tgacgatatc ttgtgaagtt gtttcatcta gatttccagt
     3781 aggctcatcc gctacgatta gttgatgttc acagcagata gcacgaacga tagcgactcg
     3841 ctgctgctga cctcccgaaa gtttggtcac ctgttttttt gctaatattt catcaattcc
     3901 tacacgagcc agtcgatcta aagcaacttg tcgataattc tcttgcttgg tcttagtgat
     3961 ttccattgct gctatgacgt tgtctagagc cgataaataa ggaagcaagt tgtaagcttg
     4021 gaaaataatc gagacatcat ttttacgata acttcttaac ccgattttgt tcagtgaacg
     4081 atcttgatag tatacctttc cttcttttgg tgcatctagt ccagaaataa gcgaaagaaa
     4141 agtcgttttt ccagaaccac ttgctcctaa aatagcgtac atttttccag cttcgaaatt
     4201 cagttctaca tctttataaa gcgcatcttg cggatcttga taccaaaatc ccaattgttc
     4261 tgtccgtaac atcaaattcc tcctttagtt taataggatt tttcttgggt ttaatcttaa
     4321 tatccctata gaagctaaaa agacagaaaa cagactgatt ccaaatgcta ctccggctaa
     4381 tagtcccaat tgtgcaggtt tgacattgat ttccaattcg ctgacttgat cagaaacagc
     4441 aaatggatta ttcgtggttc cttgttgacc atcgcgttga ccgccttgtt cttgtctgtt
     4501 acctggcatt tgtccaccgc caggagcttg ctggttttgt gtcgtggttt ctgtctgttg
     4561 gtcaagtagc tgctgtccca cggcattcgc gacgacgttt ccgctaagcg aagcaactcc
     4621 taatgcaaga atcatacaaa tagccacttc ggtaaacaat tggagaatga ttttacttct
     4681 ggattctcct aaagatagca agacgcctaa ctcatgccga cgctcccgaa tggtaatcat
     4741 aatgatcaga gtaagtatga taataccggc tacagccact agtaaaacaa catttttaga
     4801 aaaacttgcc acgttgttta aaggttccaa cattgactga tacatggaat catttgattg
     4861 taagctaaat gtatctgtgt cgatcagctt ctcagcttct ttgagaaact catccatttt
     4921 atctggatca gatagagtgt aaatagcaga atctatcgtg ttatcgtcag atgtcccatt
     4981 tagttcattt gcaaaagtgt aagaagtgta aagtgtattc gacggattca tgaagttgaa
     5041 tttcataccc atactgccgc ttgtttcgct ggattcataa attcctttga tcgtcatttc
     5101 ataagtggta tcttcatctt cagtactcgt gattgtgaaa gtatctccga ctgccaggtc
     5161 atttgtttct gctagagtag aatcaatgac tacattgttt gtgcctttgt cgctgtcatc
     5221 tattccttca ccatccgtga tttttgctgt tccatcagaa aaattactat tttgtgcagt
     5281 tgctgacaca ccactgattt gaaagtctgc ttgagtcatt tgcggattcc cgcccattcc
     5341 tccagcaggt tcaccgcctg catcagtagt ttctgaactg tcttcacttg aatcagaact
     5401 agtgatggca gttattccat cttttgccat agctgaggcg gaactttcaa aggaataact
     5461 ctttacatta tctaattcag ctattttttc cgcgtccgtt aaggaaactg gtgtaagaga
     5521 aaaactgcct ggatctggac gttcttcagt ggtagaaccg cttgaagaag tttgttcctg
     5581 ttttttaaat gaagcttctc gattggcact taaagtcact gttgctccaa cttctttttg
     5641 tgcttgattc gctgcttctt ctgcagcgct tcgaatcgtg aggccagcta gcacaaaaat
     5701 aagaatggct gtgaacacgg caaacaataa caatgaacgt ccccatttta cttttgtact
     5761 tttccatgca cgtttcataa aattcatatt ttgcatccct cctagtagat gcatttgata
     5821 atagacaacc tatcttaaag ctagcttaaa tcttatgctg tttgcacacc atcaacatga
     5881 ggttctgtat atataaaaag tcactatagt tattgcaaaa aatgtggtaa aatgaaaaaa
     5941 gtatgtttca aaccgacgaa gaagaaatga aagaggaata gcaaaagaaa tcctagatgc
     6001 tacagaggga tttttacgtg catagttgtt ctaacaattg ctgatactag cgatttagtt
     6061 tatttgttgt ataataatgg ggaacagctg aatacttata taagtattct ggctatagcg
     6121 tttcgcaaaa gatccatttt gttaatgaag tatatccaaa ataaaatgcc catccaactg
     6181 tcagaacgcg gatgtgctgg taattttcct gctgattctc cataagttga gaaatagtga
     6241 ttttgcatat gcttcattgg aatggctcat atatttagat aaagaagaaa atcgacatct
     6301 ttaaacttgg atgtcataag aagaggtgaa gtttgtgagt accattaaaa ttatcccatt
     6361 aggcggcgtt cgtgaaaatg gaaaaaacat gtatgtcgca gaagtgggcg aggatatttt
     6421 tattttagat tgtgggctaa aatatcctga aaatgagttg ttggggatcg acgtcgtaat
     6481 ccctgatttt acgtatttag aagaaaacat tgatcgtgta gctggtgtct ttttaacaca
     6541 tggacatgca gatgcgatcg gggcacttcc ttacttgttg tcaaaaattt ctgtaccagt
     6601 attcgggaca gagttgacga tcgagttagc aaaattgaat gtcagtcgta atgaagcagc
     6661 caaaggattt aaggatttcc atgtggtaga tgagcataca gaaatagact ttggtgaaac
     6721 agtagtgagc tttttccgta ccactcatac gataccagat tcagtgggga tcaatctgaa
     6781 aacaaaagaa ggtagtatcg tttatacggg agattttaaa tttgaccaaa cggctatccc
     6841 aatgtatcaa acagattatg cacgattggc ggaaatcggc aaagaaggag tcttagcctt
     6901 actaagtgaa tcggcaaatg cagaaaatcc tcagcctgta gcttctgaag cccaaatagc
     6961 agatgaagta tttgatacga tcaaatattg ggaagggcgt atcatcgtcg cttgtgtagc
     7021 aagtaatttg caacgagtgc aacaagtatt ggatgcagca gaaaaagcac aacgaaaagt
     7081 tgtcttgact ggtcaagatt tcgaacgaat tattcgcaca gcgatgcgtt tagaaaaact
     7141 tcaattacca agtgaagatc tgcttgttac gttgaaagac atgaaaaact atgatccaga
     7201 agaactgatc attttagaaa ctggccgaat gggtgaaccc atcaagtctc tgcaaaaaat
     7261 ggcaaacggt acacaccgta atgtcaagat tgaagaagga gatttggttt atatcactac
     7321 tacgccaagc atcgcgatgg aaaccatcgt ggcgaaaacg gaagatatca tttatcgagc
     7381 tggtggaaca gtgaagttga tctctgagaa tatgcgcgtt tctggccatg ccaatcctaa
     7441 tgacttgcaa ttaatgatca atttgatgaa accaacatat tttgtgccag ttcaaggaga
     7501 gtatcgtgaa ttagctgctc atgcagattt ggctcatgca gtagggatgc catacaaaaa
     7561 tatctatatt actggacgcg gagacgtgct ggaatataag aacaagcgta tgagtgttgc
     7621 aggaactaca agtgcagaaa atgtaatgat tgatggatta ggcataggtg atatcggaaa
     7681 cattgtttta cgtgatcgaa aaatcttgtc agaagacggg attttcgtag cggttgtcac
     7741 tatcagtcgg cgagaaaaga aaatcatttc aaaacctcaa attacttcac ggggattcgt
     7801 ttacgtaaaa gctagccgtg atctgatgaa agaaagcagc aatatgattg aaacaatcgt
     7861 tgagaaacat ttagaaagca atgattttga gtggagcaaa ttaaagcaag acatccgaga
     7921 tcaattaggc cgttacttat ttgaacaaac aaaacgccgc ccagtgatct tgccagtcat
     7981 tatggaagct actcaacgtc gaaataaaaa ataaacaaaa agagtaagat gaacctctct
     8041 atagagtgct cgtcttgctc tttttttgcg aaaaacaaat aatttttgaa tacagcatcc
     8101 ctcgctttat aataaacgat agaataaagg gagtgaatca atgatgagtc aaacaaaagt
     8161 cgaacgttat ttagaagaac aaaagatcag ctatcaacca cttgatttta gtcatgtggt
     8221 agaagaatct ttcacagaag aactacaaaa aagagggatt gatccagctt tgatatgtaa
     8281 aactctcgta gtgaaaggaa acaaaactgg ggtgatcatc gcattgattc ctttgaacga
     8341 tcacttagat tacaaaaaaa cgagacaagt gacgaaagat cgtaaagttg gttttcctgg
     8401 aatggatttt gtattaatgc atacaggata tccccacgga gcaaacaccc ctattggaat
     8461 aaaactcgca catcctgatt attgtttttt agcagacagt tctttaaaag aaaaagaaga
     8521 agtaattgtg tcaagcggtg aaatcgggag aagccttcgg ataaaagtag ccgatttaga
     8581 aaagatcgtc catcctattt acgctgattt aattagttaa gggacgagaa gagaagctaa
     8641 gcagagcaga gacctcaaac ttgagattat atctgcttgg tacttggtgc atcctgtatt
     8701 atctgttaca attgctttgt acatactgta ctatcaatcc aatgaaaata tatttagcag
     8761 ctatattttt tcattgatgt gcaagtgatg aaatcgtttt taataaaaaa gatacagaga
     8821 aaacgcgagg tgtaaaatat gaaaattacg tatcatggac attcctgttt aacaattgaa
     8881 atggaaaatg gccaacgcgt gatgattgac ccgtttatta caggaaatcc attgacagac
     8941 ttaactgcag aagaagtaaa gacagattgg ttgctgatta ctcatggtca tagtgaccac
     9001 ataggagata tgattcctat tgcaaaagaa aatcaagcca caattatcag tattgttgag
     9061 attgctgatt atgcgaacag cagaggagta gattcattcg gtatgaatat cggtggaaaa
     9121 catgcgtttc cattcggcgt ggtaaaattc gtacatgcac agcatagttc aagctatgaa
     9181 gtagacggta tcgttcagta tatgggcgag cctagcggaa ttattattca agcagaagga
     9241 aaaacgattt atcatgcagg ggatactgct tatttcagtg atctaggatt gcttgcagaa
     9301 gaattcgata ttgatgtcgc tttcttgccg attggcgata actatacgat ggggccagaa
     9361 gatgcagcaa aggctgcgca aacaattcgt gccaagaaag tagttccaat ccattacaat
     9421 actttccctg tcattgaaca agatcccgaa tcttttgtag agttactgga agatagtgaa
     9481 ggaaaaataa tgagcgtctc ggaaacgctg gaagtgtagg cagaaaatgg cgacaaaaca
     9541 tgatttgatt ttagaatata ttgaaagttt acctgttggc aatcgaattt ctgtaagaag
     9601 catcgcaaaa gaattgaatg tcagtgaagg aacagcgtac cgtgcaatca aagatgcaga
     9661 aaatgtcgga ctagtttcaa caattcaacg agtagggacc atccgtatcg aacgcaaatt
     9721 aaaaaaacat attgaacgtt tgacatttgg cgaggtcgtc aggatcattg aaggggacgt
     9781 tttaggcggg gcggcaggat tagacaaggt cttgaataaa ttcgtcatcg gcgcgatgac
     9841 taaacatgcc atggaacgct atattacgcc aggatcacta atgatcgtcg gcaaccgggc
     9901 ggaagtccaa aaattagcat tggaagatgg cgccgcagta ttgatcacag gaggattcaa
     9961 tacgactgct gagattgcaa agctagctga tgatcttgaa atgccgatct tacgcacctc
    10021 ttatgataca tttacagtcg caaccatgat caaccgagcg ttaagcgacc aattgatcaa
    10081 aaaagatatc atgcttgtca gtgatatcta tatgccagct gaaaagactc attatttgca
    10141 gcagacagat acgatttctg attatcagcg tttgtcagaa gaaacgcagc attcgcgttt
    10201 tccagtcgtc aaccgccatc atcgtttgat ggggattgtt acggcaaaag atgttttagg
    10261 gaaatcgcca aaccagatca tcgatcgggt gatgacgaaa gaaccaatca gtgtaaagaa
    10321 aacaatgagt attgcctctg ttagccatca aatgatctgg gatgggctag aagtcatgcc
    10381 ggtcgtttcc gatgacttga cattggaagg cttgattacc cggcaagatg taatgaaagc
    10441 gatgcagctt gtccagcgac aaccgcagat tgcagatacg atttcggatc agatatctgg
    10501 aaatatccat acgattgata ctgaccgtga aggaaatcgt ttggaaaacc ctaagttcaa
    10561 attcgtagta gcaccacaaa tggtcaatgg cgttggaacc atttcttttg gcgtacttag
    10621 cgaaatcatt tctgatgtcg cacaaaaaac aatggtgatg aaccaaaaac gaaatatctt
    10681 gattgaacaa gtgaacctac attatttgcg gctgatccaa ttggaaagcg aattagacat
    10741 tcgtccacgt gtattggaaa tcggcagacg ttccgctaaa ctggatatcg aagtgtttat
    10801 tgaaaacacc atcgtttcca aagcaatcgt tgtttgtcaa gtaatggaac gttcttagaa
    10861 agcaggagtg aaatgacagt acagcaagaa atagtagata caataaaaaa atacgataaa
    10921 atcattatcc acaggcattt gcgccctgat cctgatgcat taggatcaca agtaggatta
    10981 gccgaactat tacgggaaag tttcccagaa aaagaaatac gtcaagtagg tgaaacgatt
    11041 gaagggcttc gctttttagc agaaatgcaa gaagttgaag atgtcttcta ccaaggagcg
    11101 ctagtcatcg tgactgatac agctaattca cccagaatca gtgatcaaag atatcagttg
    11161 gcagaaaaac tgatcaaaat cgaccatcat cccaatgatg acccttatgg agatcttgtg
    11221 tgggtaaata caaaagcaag tagctgtagt gagatgattg cagaatttgc tttgatgttt
    11281 cctgaatctt taaagatgaa cgttaaggct gcacggttac tttatgcagg aatcgtcgga
    11341 gatacagggc gctttttgta tccctccacg acgtcccgta cattagagat tgcttcgata
    11401 ttgagaaatt atccatttga tgcaagtgct ttgaacagag agattgaaca aatgccgatg
    11461 aaagtcgcta aactttccgg ttatctttat caaaatatcc aggtggatga aaatggtgca
    11521 ggaagaataa tccttccgca agaaattctt gataaatatg gaatcgatga ttcagagaca
    11581 gcttcgatcg tttcacttcc tggagtgatt gacgaagtgc tggcttgggg gatttttgtt
    11641 cagcagcctt cagggtatta ccgcgtacgt ttacgttcta aggggccgat catcaatgaa
    11701 ttggctaaac gccatcatgg aggaggccat ccattagcaa gtggtgcgaa tgcaaaagat
    11761 ttagcagaag tagaggaaat ctataaagag atccaagata tatgccaatc gtataagagc
    11821 agaaaaacag aatgatactt cgttcgaaaa cgtgcctttt gtagtaaaaa tcacaaatgt
    11881 atcttttcaa gatcttcttt ttccgttaca atagtgctca ctaaagattt taaaggagaa
    11941 agaagatgac attaccaaat tgcccagaat gtggctcaga gtacacatat gaagatcgag
    12001 gcttgttgat ttgcccagaa tgcgggaacg aatggaacaa agaagaacaa gaaactgtca
    12061 tagaagacgg attagtggta ttagatgcaa atggaaaccg tctgttagag ggcgatagcg
    12121 tgacagtgat taaagacttg aaagtaaaag gtgcgtcagg tgccattaaa caaggaacaa
    12181 aagtcaagaa catccgttta gtagaaggcg atcataatat cgattgcaag gtagaaggat
    12241 ttggaccgat gaaactgaaa tcggaatttg tgaaaaagaa ttaaagaata ttcaaggtct
    12301 tttcacattc ttttcctact actaatgata aaataaagta aacgaaaagg agtgaaacga
    12361 aaatgtatgg ttatggaatg tatggttttt tagatccgac atttcttttg gtcattcttg
    12421 gattagttat ttccggaatt gcgtcagcgt acgtcaatag tactttccga aaatacgatc
    12481 aagtgagaag caaaaacaat gtgactggta cacaagcagc acaatatata ttacaaagtc
    12541 aacagataaa taatgtcggt gttcaacaaa ttgccggaga tctcacagat aactacaatt
    12601 caggtaataa gatgttgagt ttgtctcaag caactgcaca gtctacttca gttgctgcga
    12661 taggtgtagc agctcatgaa tgcggacatg cggtacaaga tgcttcgggt tatgtcccat
    12721 tgaagattcg tgcagcactt gttccagcag ctaactttgg ttcgatgatt tcgttcccct
    12781 tgattatggt aggtgtattg cttagctgga actctacgtt aatcaatatc gggattttcg
    12841 ctttttcttt agcattactt ttccaattgg tgacattacc agtcgaattt aacgcgtctc
    12901 ggcgagcaat ccaaattttg agtgaaggcg gactactgac ggaagaagaa gtaccgatgg
    12961 caagacacgt attatttgca gcagcactga cttatgttgc agcagcgctt tcgacattcc
    13021 tgcagctgtt gcgcttgatc ttattgttcg gcggcaaccg aagagattaa aataaagaag
    13081 ttgtgaaatc agcgctttga tctgagctaa taccgagagt tagctgattg aataccattt
    13141 atttggaata agaggatgtg acaagaattt tgtcacatcc tctttgtttt gttctcataa
    13201 ccttttttca tatacagttc atgaaccgct tttattaact tgccaaataa cactgtattg
    13261 tgcgttgtat ccagatagaa agtctccgtt tcatctgaaa ttttctcatg ctgatctgta
    13321 atgatcaaat cagcatgttc tgaatatgcg gtaattttaa cagcatcttt gcgaaataac
    13381 tgctgcaaat ggctttgaag atgataattt cctgtaaact gtttggttat ctgcaaatgg
    13441 atgagtaact gctctttcgt ttctgtttgt agtaagtgtt ggatcaattg actaaggtat
    13501 tgactgattt ggtttctttc cccattt
//
LOCUS       DAXWOO010000070        13405 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.070, whole genome shotgun sequence.
ACCESSION   DAXWOO010000070 DAXWOO010000000
VERSION     DAXWOO010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.070"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(65..607)
                     /locus_tag="ACGE70_001567"
     CDS             complement(65..607)
                     /locus_tag="ACGE70_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFF8693209.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            742..1131
                     /locus_tag="ACGE70_001568"
     CDS             742..1131
                     /locus_tag="ACGE70_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693210.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1135..1899
                     /locus_tag="ACGE70_001569"
     CDS             1135..1899
                     /locus_tag="ACGE70_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFF8693211.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            2028..2975
                     /locus_tag="ACGE70_001570"
     CDS             2028..2975
                     /locus_tag="ACGE70_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8693212.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3105..3845
                     /locus_tag="ACGE70_001571"
     CDS             3105..3845
                     /locus_tag="ACGE70_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693213.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(4136..4804)
                     /locus_tag="ACGE70_001572"
     CDS             complement(4136..4804)
                     /locus_tag="ACGE70_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFF8693214.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            4938..5225
                     /locus_tag="ACGE70_001573"
     CDS             4938..5225
                     /locus_tag="ACGE70_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693215.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            5641..7179
                     /locus_tag="ACGE70_001574"
     CDS             5641..7179
                     /locus_tag="ACGE70_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFF8693216.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            7176..8141
                     /locus_tag="ACGE70_001575"
     CDS             7176..8141
                     /locus_tag="ACGE70_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8693217.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            8128..9450
                     /locus_tag="ACGE70_001576"
     CDS             8128..9450
                     /locus_tag="ACGE70_001576"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFF8693218.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      9513..9617
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            9691..10434
                     /locus_tag="ACGE70_001577"
     CDS             9691..10434
                     /locus_tag="ACGE70_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8693219.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            10449..11441
                     /locus_tag="ACGE70_001578"
     CDS             10449..11441
                     /locus_tag="ACGE70_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFF8693220.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            11453..12190
                     /locus_tag="ACGE70_001579"
     CDS             11453..12190
                     /locus_tag="ACGE70_001579"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8693221.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            12180..12902
                     /locus_tag="ACGE70_001580"
     CDS             12180..12902
                     /locus_tag="ACGE70_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFF8693222.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(12986..>13405)
                     /locus_tag="ACGE70_001581"
     CDS             complement(12986..>13405)
                     /locus_tag="ACGE70_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFF8693223.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         4416 a         2294 c         2786 g         3909 t
ORIGIN      
        1 taaataaaaa ctgtctaaca tttaaaattt aatttagata ttagacagtt tttaagtttg
       61 tgagttattt tccttgtcgt tttctcactt ttttagtcca gaaaccacct gaaatatatt
      121 ttggctcata agaaatgata aaagcacgct cttcgatttc tttgatcagg cgatacagtt
      181 cacgttcgct ttttctagga gaaagtattt cgagaactag ccgctcacct tccagcccca
      241 ttgcagaagt ctgtgtcaca ccataaccgt gttctctcag tatacccggc aaacttttat
      301 cttctgcgct atttgctggc aaaatcgttg tgaccatgat gtatcctaat gccaatttgt
      361 cttcaatcaa aattccgaca ctgataccta ctgcgtaacc taacgcatag acaaataaat
      421 tcagtggatt atctaaacga tctaaaacaa gggataagcc gacgatataa atcgtgatct
      481 cgaccatact gacaagtgga gcaatcagtc gatagccttt cattgtcaac atgaaacgta
      541 acgtgttcag agtgatataa gcaaaattaa tgaggaagat catccccaac atttttggat
      601 caatcatttg taaaactcct ttcatttata taacaacaat atcaaaaaaa atcgtaagtg
      661 aagaaaaatt gaaactttat acatacaaaa aaatgataaa atgattataa tagaattata
      721 aagataggtt aggtgattaa aatgaaaacg gtcaatcgtg tgttttgtgc cagccacgtg
      781 ctaagtctcc ttctcagtat atacttaggt gtctattcga ttcaacagcc aattgaggta
      841 gagaacattg tttttcagat gtcgctgaca gatggtttaa ttttaagcgt ttttttcttt
      901 ttgatactgt ttattggtaa tattttcggt agcgttgcca gttttttgaa tttttctatt
      961 tatccactcc tttcgctagc attaggagtg gtgggacttt gttctctgtg cttatttccc
     1021 gagccattca gtcctgcatt tatattattt ggtacactga acctgttcca gacaactgtt
     1081 ggagcatggt tgctatggcg atcaggaaat ttaatgaaaa taggtgagta acaattgaat
     1141 ccaattatat tgaaacgttc tgtccatcat attccagtgt tagaagttgt tctgaaagaa
     1201 ttaagagagg aacgtattcc cgttgtcatc tattatcacg gatggcagac aagtaaggag
     1261 ttagttttga cgcaaggaag aaaaattgcg cagcagggca ttcgtgtgat cctgccagat
     1321 gcgatgaatc atggggagcg caaacagccg gtttcttcta ttccatccta tacattttgg
     1381 gatagtatct acggaaatct gtttgaattt gacacactta tcaagcattt agaaaaaaga
     1441 ggtcttctac atgatcagct tgcagttggc ggggtttcga tggggggaat gacaacatgc
     1501 gccttgttgg ctaaacatcc tcatatagta gcgggcgtgt gtttgatggg ttcccctgca
     1561 cctttggaat atggaaaaga aatcgtaagg cgcgcatcgg aatataatta tgtcgtacca
     1621 aaagactatt ttgatttgat ttcatgggtt aagagctatg atttgtcatt gcagccggaa
     1681 aagttagctg gacgtcctct gttcttttgg catggacaag ctgacgaaaa aatcccttat
     1741 atacaaacgg aacgatttgt agaaaaaaat cggcataagt cttacggaaa agaaattgtt
     1801 tttcgttcct caaaaacaca gcgacatatg gtacaaatac cactaatgga agaagcttcc
     1861 acttttttag caaaaaattt gataaaaaag tataaataat tgaaacggaa aatcactcat
     1921 gacgatagag gattttccgt tttttatttt gggctataat aaactctgtt aggaaaattt
     1981 ggtataattg atagtaagaa tgacaacatg ttagaaagga agagagcatg cccactttag
     2041 cagatgttgc aaaacgtgcc aatgtttcaa aaatgacggt ctctcgagtc atcaatcatc
     2101 cggaacaggt gactgacgaa ttgaaagagt tagttttctc agcaatggct gaactggatt
     2161 accgtccaaa tattgctgca aaagcacttg tcacgaatcg gtcgcagatc atcaaattat
     2221 ttatactgga agaaatcgac acaactgagc cctattacat gaatttgctg atgggaatcg
     2281 ctaaacggat cggtaaagcg cattattctc tccaattagt tacaaatgat gccttcgacg
     2341 tcggttcttg tgatggatac atcatcacag gtgtcagaaa aaaagacttc gattggatcg
     2401 accgtttgga aaaaccagtt gtcttgtttg gggaaaattc tgaaggatat gattatgtag
     2461 acagcgataa tgaatatggt acagcaaaag caacgcaata tgccttagaa agagattatc
     2521 aaacgattat ttatatcggt atcgaagtgg acgagccttt tgaaatttca agggaaactg
     2581 gttatttatc tgtgatgaag gaagttgatc ttcctagtaa aatcttgcgg tttccaaatc
     2641 attcgacaga agtagaaaga tatatgacag aaaactgggc agattttaca gataaaactt
     2701 gttttgtatg tagttcagat cggctagcac taggagcagt aagagggatt ataaatgctg
     2761 gaggacactt gcccgaagaa tttggtgtga ttggatttga tggggtattt ttagaccaaa
     2821 tcagttcacc taaattgaca acgatcaagc aagatattat acgtatgggt gaagtatgtg
     2881 gagaaatgtt gttaaaaaaa atagaagaaa aaggggcgag tcaaggttat cgtcgttttt
     2941 ttcctgaact agttatcaga gagacaacga attaagtctt attactatat atatatttat
     3001 gaaattcatc gctattttct taaaataatc agcgcattgc tactgaaaac aatgaatctg
     3061 tgctattatt gagaaaagtt ccatttttga gaaggatggt ttgaatgaaa gaaatcagac
     3121 gaaaacattt ccgttttcct gcgtacgatg atgagttagg cgtaaaatta accaacgaca
     3181 agaagcgagt attatttgac ggacaagaag atcttttgac tgaacagaat cagtccccgt
     3241 ttgaacagat gaaagaattt catttcacga acacgaacac cgagaaaatc cgtcgcaagc
     3301 gtcgtgcaac aaatggattg aagtcagctc cgcgtaagga aaatttggaa aagcataaag
     3361 gaaaattacc ggattatacc ggccattacc aatcagaaat cacatcgaca ggacgaaaaa
     3421 aactttttgg tgattcctct cgttctgtca gacaaagtga aacaaggaca gagtccaagc
     3481 atactacacg aaacgcatct gttgacacga aggtgcggga gcgttcttat tttgttccta
     3541 aatatgtacc cgcatcgatt atcccagacc ctagagaaag caaaatttct gaggatcatt
     3601 taatcgaatc gatgaaaaaa acggatgact cttatctgtt gttcgataca gaaccgacgc
     3661 cgtatcaaga aaaaagaaat ggtgagccgt ctgtacgaac attcaatcga acaaaaacgt
     3721 tagaggaaac aaagcagtca gaagaaaaaa aatcgaaaaa aagccatggt atcttagaac
     3781 gctcattaca aggattgatc gatgatcaag gaaactcttt gggagaaaat agctatttta
     3841 aataagtgat atacaaattt atattcggct acagttggaa aggaaacgct gcggcgtttc
     3901 cttattttat actctatccg aaagtttata tcgtatcctc aaattttttt gagtttaagc
     3961 gaattttttc tcccaataag taagaaaata atggcaaaat aaaaaaacaa actattttcg
     4021 cagatcgttt gcaaaaatag tttatttttt tataaagaag attttactga ataatatcat
     4081 tttagtttac ataatgatat ttctgtaaat taaataaagt agtgaaaaat aagaatcagt
     4141 gctctaactg ttcaagagga tcaagtggtt caaatgtttc tgtagtctgt tttgcttcca
     4201 attggggcaa aacagatcca atttccagat acttcacttt attcaatgta taagatagaa
     4261 tagaacagct aagtaatgta tacagaaaat gtgtgagatc caaataaagc aaggagacaa
     4321 ctaaaatacc aaaagtaatc aagaaaagac ttttgccaat cttaatatga aaatattttt
     4381 caatggcttg ataaatgata tctgttccgc ctgtgcttcc gccgaatcga aagacgagac
     4441 cgcaaccaat gccagaaaga atacctgtca tcaaactatt gatcagtaga ctatctacag
     4501 caaaatgtat atgaaggctc tcccataaat gtaaaaagaa aactagcgca aatgttccga
     4561 cgatcgtccg gagaaaagcg tgttttccaa aaagaaacaa tgagataagc aataaaggta
     4621 aattaaaaag cagtgaagtg acagaaggag cgaatccaaa aagattatgc aaaagcaaag
     4681 caataccagt aaccccacca tctgccagct tagatggaat attgattcct gtcacagcta
     4741 atccgtagat tcctgcgcct aaaatgattc cgcacaattc tgtaaataaa tttaattttt
     4801 tcatttctag cctcttttct tttgaaaatt ttcagcaact tctttattat actgacttat
     4861 actcttactt caagaggaaa atgaaaaaaa attatttttg gctgtttctt tttatttatc
     4921 aaataaggag tggaaaaatg cgagcagcag acttattgac aattcttaca aaatcaccca
     4981 aaaaaacaga gtatcaagga gtttttttgg ctcaagaaca aaaactggtg cctgtgacaa
     5041 tggtccagat aaataaagca aatcaattta tctttttatt tgaacacggc aaagcaccac
     5101 ttgctatgaa agacatcttt ctttttttga tgaagaaccg tgagaaagct gtttattatc
     5161 agtcaaaaga agaactcatt cctctttatg gagtgaaaga gatagaaaat aaagtggtga
     5221 tataagtaaa aataagaaaa caagacgttt atgatgtcaa aacaaccatt ttttacattt
     5281 ttggcaaaaa agtgtcatga tacagtataa tattcgttga acaggccaat aaaggtgttc
     5341 tcccccacga gatttctact ttattggctg tttcttttta ttaaaataaa agttgtgtgt
     5401 gtaaaaaaga tatagctgat tccaagtgac aatttcgtat agatttttat ggtattcgta
     5461 gtgaaaaaac gagaaccaaa aagttgatcc gactttgttg cttggattct ttttttgtca
     5521 gcatatttag aggtattttt ttcttttttt tgttcgtgct gtaaggttaa ggcaagacaa
     5581 gttagttggg aaaaaggaaa gaaatgttta tacttaaaga ataacagaaa ggaaggtcgc
     5641 atgaataatt ttatacaaac attaagtgaa agaaaaggcg agttgctgga agctgcattt
     5701 caacatctgt ccatttctct catttctttg ctgattgcag cactgattgc tattccttta
     5761 gcgatttggg cagccaatca taaaaaaatg gcagaagtct tattgcagat cacgggtgtg
     5821 ctccaaacga ttccttcact tgccttatta ggtttactta tcccatttgt agggatcgga
     5881 actgttccag cattgattgc acttgttatt tacgctttgc ttccgatttt ccaaaacacg
     5941 tatattggtc tagctgaaat tgacccgtct attgaagaag cagcggtagc atttggtatg
     6001 tccagaatga gacgcttgct gaaagtagag cttcccatcg cattacctgt cattatctca
     6061 ggtatccgaa ctgccttagt tttaatcatc ggtacggcta ctttggcagc attgatcgga
     6121 gcaggtggat tagggacatt tattttgtta ggtattgacc gaaatacacc tgttttgaca
     6181 ttgattggtg cgatcagttc tgccttgctt gcaattatat tcagcggact gattcgctgg
     6241 ttgcagcata agaaacctcg atattccatc attactttag cagcagtggt gtgtttattt
     6301 ggcggcgttt ctttatatca gtctgccttg tttaaaaatg aaaccatcac gattgctgga
     6361 aaattaggag cggaacctga aatcctgatc caaatgtata aagaattgat tgaagacaac
     6421 acaaaaacag aagttgaatt aaaaccgaat tttggcaaga cgagtttttt gttcaatgca
     6481 ctggatcaag gtcaaatcga tatctatcct gagttcacag gtactgtcct agaaagcttg
     6541 gtgaaggtac ctgaagcaat gaaaagtgct gatttgacaa aagaaaaaac atatgacgaa
     6601 gcagaagaat tattgaataa gcaattcgat atgacattat taaaaccaat ggcttaccaa
     6661 aatacgtatg ccttggcagt caagagatct tttgcgaacg aacatcaact taaaaagatt
     6721 tccgatttgg cagctatctc tgatcagatc aaagcgggat tcacactgga atttattgat
     6781 cgttcagatg gatataaagg aatccaagaa gcatatggct tgaatttttc aagtgtacaa
     6841 agcatggagc caagtttgcg ttaccaagca atcaataatg gagatgtcaa tattgtggat
     6901 gcctattcca cagatagcca gctcaaggag tacgatctag tgactctaga agatgatcga
     6961 gggttattcc cagcttatca aggcgcaccg ctgatgaaaa aagattttgc taaagaacat
     7021 cctgaaatcg ttaaagcact gaataaatta agcggaaaaa taacagagga tcaaatgatt
     7081 gatatgaatt accaagtcaa tgtcgaaaag aaaaagccga cagaagtagc acgaatcttc
     7141 ctgaaaaatg aaggattagt aaaggaggaa gatcagtgac taaattaatc gagttcaaac
     7201 atgtgcaaaa aaagtatgat ggaaagtatg tcatcgatga cctaaatctg gcaatcaaaa
     7261 aaggagaaat ctttgttttg gttggtcctt ccggtagtgg aaaaacaact actttgaaaa
     7321 tgatcaatgg attatctaaa ccatcagcag gagacatcta cttcaaagga aaatcgttaa
     7381 atgaatataa tctgcagaaa atgcggtgga atatgggata tgtgctgcag caaatcgctt
     7441 tgttcccgac catgacagtc aagcaaaata ttgaagtcat ccctgaaatg ttaggttggg
     7501 aaaagcagaa aagagcagac cgtgtagatg aacttcttca aaaagttggc ttatctcctg
     7561 atatttatcg agatcgtatg cctagagagt tgtctggagg agaacagcaa cggatcggga
     7621 tcatccgagc tattgctgca agcccagatg tgattttgat ggacgagcca tttagcgcat
     7681 tagatccgat ttcaagaaac tcattacaag aattggtttt gtccttacac gaagaattgg
     7741 gcacgaccat cgttttcgtt acgcacaata tggaagaagc aatcaagcta ggtgatcgta
     7801 ttgcatttat gaaggatgga gaaattatcc aatgtgacac accagagcag ttattgatga
     7861 atcctaaaaa cgactacgtt cggcatttct ttgatgaacc gaaacagacg aaagagtggc
     7921 gagtagaaga tttagtcgtc aatggatatt tcttaaatga aataccagaa aatgctcgtc
     7981 aacaagtttg ctttgatacg cctatgaaag aagtatattc gcttctttcg gtgtatccaa
     8041 gcattgtgat agtagaaaag caaagaagta ttgggtcgct gacaagtaaa gaaatctttg
     8101 cttttttaag tcgagaggag gaaggaaatg aaaacatatg atgcaatcat catcggttca
     8161 ggtgtgagcg gcttaagtgc tgcttatgga ttgaaagaag caggtaaaac agtactagtg
     8221 gtcgaagaag atctatgggg cgggacttgt cctaaccgtg gatgtgatcc taaaaaagtc
     8281 ttgttaagtg cagtagaggc acgtaatcgg gtaaaacaat tgagtggaaa aggttttaac
     8341 gagatcccaa ctgccaactg ggaagaactt caaaaattca aacgaacatt tacggatcca
     8401 gtcccagaaa gccgaaaaaa acaattagca gaagctgaaa tcgatcatct ttcaggaacc
     8461 gctcgttttt tggatgattc atctattgaa gtcaacgaag aagtcttcca tgcggattac
     8521 ttagttttgg caaccgggca aagacctacg atcttaccag tggagggaaa ggaatacttg
     8581 aaaactagtg cagacttttt atcattgcct gtactgccta aagaaatcat ttttatcggt
     8641 ggtggttata ttgcttttga gctagctacg attgccaatg ctgcagggag caaagtaacc
     8701 attgtccatc ataatcaacg gccattaaaa gaatttgagg cgtctttggt agaggaagcc
     8761 gtccatcaaa tggaagcatc tggcattcag tttgcttttg gtgttgaaac ccaaaagatc
     8821 atttcagaag gtactcgcta tcgtttagtc ggaaaagaga cggaattagt agcagatatg
     8881 attttttgtg cgactgggag acagccgaat acagaatctt tagcacttga gcaagcgaat
     8941 atcgtctttg ataaacacgg aattgcagtc aatgattatc tgcaaacgag caatccaaaa
     9001 atttttgcgt gtggagatat tgtttcaagg aaaaccccaa aattgacacc agtcgccact
     9061 tttgaaggaa attatgtagc taagcggata acggatgcaa caagcgaacc tatcaaatat
     9121 ccgattatac ctacgatcgt ctacgccagt cctaaacttg ctgaagtagg agttacaaaa
     9181 agtcacgcct cttcgtcaga tcaagtggta gagatggatc ttaccagttg gttcacctat
     9241 catcgtgtca atgaaccagt tgctaaggct gaattgacct ttgaccagca aaattatttg
     9301 attggtgctg cagtgattag cgagcaagca gacgaattga tcgatgattt gacactcgtc
     9361 atcaatcaaa aattgactaa aaaagagttg gatagctata tcatggggta tccgacattg
     9421 gcaagtgatc tctcctattt attgaaataa tcagcatgat aactaaagcg cttgttgctt
     9481 tttccttctt gtgctacact ttaaaaaatg aatacctgtt tctggggatg tcgtagacga
     9541 catgagagta agttgtgaac ttagacccat tgaacctgtc agttaatact ggtgaaggga
     9601 gaaacatagc agacgcttga agcgggttac tatgtcgtgc ccatttcaag cgttttttgt
     9661 tattcatatt agaagaaagg aggtaacaaa atgaagaaaa atctttatcc agcagtcagt
     9721 atggcattca ttttaattat ttggcagatt gttgtcatag tttggaaaat acctgccttt
     9781 attttacccg gacctgctgc tgtattttct gcattagcgg cagatgcaca agtattactt
     9841 tcccattctg tcacgacatt aaatgaagca gtgcttggtt tggtaatagc tagcgttctt
     9901 tcttttatta ctgccttggc aatggattat tggaagtttt tagaaatgtc tttttatccg
     9961 ttactagtga tttcacaaat gttgccaatc atggtgttag ggccattact taccctatgg
    10021 tttggtttcg gtatgcttcc aaaagtgatc cttgttgtct tgataagcta ttttccgatc
    10081 gtagtttctt tttcggatac attgaggaaa gtatcaaaag agcaaatcat ctttttgaaa
    10141 acgatggggg cagatacagg gaagatttat cgaatatata aaataccagc gggtttatcc
    10201 ggcttctttt ccggtttaaa agtggctgct acttattgtg tcagcggagc agtcgttgga
    10261 gaatggctaa gcgctcaagc aggcttaggg tattacatga tacgcgtaaa aaacagctac
    10321 cagatcgata aagtttttgc tgcgatcatt tgtgtcattc tattgagttt attgctgaat
    10381 ggcgcttgtt cattattgaa aaaagggtat tatcgtatgc tttatatttt ttaagagaag
    10441 gggatcaaat gaaaaaatta tggcttttat taccgctact gttgctttta tccgcttgtg
    10501 gcactgcaaa agaaacaagt agcaaacaag aaataaagga tttgaaaaaa gtgacacttg
    10561 ttttggatta tgtaccgaat actaatcata ctggcatcta cctagcaaaa gaaaaaggct
    10621 attataaaga ggctggttta aatgtccaaa tcatcgaacc aggagataat tcgacaagta
    10681 ttggtctagt aggtgcggac aaagcacaat ttggtgtaag ttatcaagaa gatgtcactt
    10741 atgctcacgc agatggacag aatataccag tcaaagcaat tgctacagtg atcaaacata
    10801 atacttctgg attcgccacc ctatcagaca gtaacatcca ttcaccaaaa gattttgaag
    10861 gaaaaacata tgcaggttgg caaagtccaa gtgaagaagc tgtcttaaaa gctgtgatgg
    10921 aaaaagacgg cggggacttt tcaaaactga caatggtagg aagcaatgga gaagggccag
    10981 aaagcttagg aaaatcctca gatatccaat ggtactttga aggttgggat atgatcaaag
    11041 caaaagaagc gggtattgaa ctaaactata tcccattgaa agaattagat gaacggttag
    11101 attactatac tccggtaatt attacgaatg atcaactgat caaaagtgat ccagaattgg
    11161 tacagtcttt tatggatgcc acgaaaaaag gctatcagga agctattaag gatccaaatg
    11221 acagtgctaa actgttgcaa aaatatgcaa aagaaaatga tcggacgttt ttagaagaat
    11281 cacaagcatt cttgtcaaaa aattatacgg atgatcctga gaattgggga ttgatggaag
    11341 aaaaagtttg gcgcaattat acagctttta tgcaggaaaa cggattgatc aagcaagatg
    11401 taccttcgca agaactattc actaatcagt ttataaaata ggagtgatag atatgtcaat
    11461 ccaaatcagc cacttgaatg tagtcattga aaacctgaaa atattagaag acatccattt
    11521 cactatccct aaagatacct ttacatcgat tgtcgcgcca agcggagccg gtaagtcaac
    11581 attgctgaaa actttaacag gagtccagcc tatgacttct ggaacgatca aagtagacga
    11641 tcaaaaagta actggtctcc atacagcatt tagttatatg ccgcaagaag atatgttact
    11701 tccttggctg agcgtctatc aaaacgttac gttatatcaa aaaatcaatc atctaacaat
    11761 caatgaagaa caagtaaggg attatctgga tatctttggg ttaaacggct atgaacattt
    11821 tttgccggaa caattatctg gcgggatgaa gcagcgaacg gccttgttac gaacaatcat
    11881 gaatccttct tcttacttgc ttcttgatga accatttgga gcgttagatg ctatgaccag
    11941 agggcagatg caagactgga tgttgaagct gcctaaaaaa gcaaagcgga ctactttact
    12001 tgtgacgcat gatatcgaag aagcaattta tttatcagac aggattcttg tgctttccgc
    12061 ccgtccggca catgtgattg ctgagattca agtgcctgaa aaacgacggt cacgtgaatg
    12121 gctactgcag caatcagagt tgaagcaaac gatttatcag ttattggcag gtgaattaga
    12181 tgttaagtga tgctcattgt catttagatg ggagtcagag tttagcactc cttcagcagg
    12241 aacacacgat tttgacgatc atcaattgtg attcgccaga agaatggaag gaaaacagac
    12301 agcttgcagc aagtaagact caagccttaa gttacggtat ccatccttgg aaagcagata
    12361 gctatacgtt tgaacaagtt gagccttttt tgaaaaaagc aaggatcatc ggtgaaatag
    12421 ggttagataa tatttggacg aatgtaccga tgactacaca aaaaaaagta tttgagcgac
    12481 agcttgcttt tgcagccata aatgagaaac ctgttgtact gcacacaaaa ggatgcgaaa
    12541 aagagatctt aaattatatc caagcttacc ctaatcacta tttgatccat tggtattctt
    12601 ctttggacta tcaacaagac tatattgatt taggctgtta tttcagcatt ggtcttgatt
    12661 tgaaaaagaa tccagcagtc tggcaactag ctaaagcagt cccaatcgac cacttactta
    12721 ttgaaacgga tggactacat gcagcagagt gggctttagg ggggattttt tcagaaaaag
    12781 attatgtctc actgcttgaa gaacagttaa agacaatcgc tgagatcaaa gagctatcag
    12841 ttgaagaagt taaaaaaaat acttatcaaa acttggaacg tttcattaga ttaggtaaat
    12901 aaaaaagaga tgtgacacaa ctagtgtcgc acctcttttt ggctttcttt gtataaaagt
    12961 aaactgtttg attaataaat gaatcttagt accaaccgtt tgcttcccag aatgctttcg
    13021 ccgcttccca agaaccataa cgtgaagtaa catattgttc tgctactctt tcttggtttg
    13081 cagcagagta gtcaccattt aagtaagaag aatctaattg gtaacgtccg atgtaacgtc
    13141 cgttcgttgc tgtgtaagaa ccgcttgatt ctttttgtgc gatccattct tttgcagagc
    13201 ttgtgcttgc tggagtagca gcagtttctg ttacagcagg agcaggttgt gtttcagcaa
    13261 caggcgctgc ttgtttttcc gtttgaaccg tttcttgtac aacaggttgt tcagcttgga
    13321 ctggtgcttc ttgaacggct gcttgaggtt cagcagctgc ttgtgcagag ccttctgttg
    13381 ggattgttaa ttgttgtcct gagta
//
LOCUS       DAXWOO010000071        13320 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.071, whole genome shotgun sequence.
ACCESSION   DAXWOO010000071 DAXWOO010000000
VERSION     DAXWOO010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13320
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.071"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(370..1512)
                     /locus_tag="ACGE70_001582"
     CDS             complement(370..1512)
                     /locus_tag="ACGE70_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFF8693224.1"
                     /translation="MAKFEQYKKKNGDKAWKFQAYLGINPETGKPVKTTRRNFKTQRE
                     AKLALARLQSEYEDNLLTKEKPKTYKDVYDLWMTEYKRTVRGSTLLKTERIFKNHVLE
                     ELGDIYISEITPIKIQKLMDKWANKYDTAPKMMNYTGLVFKYAVRFGMIESNPTDAIR
                     KPKRRKKATVEEPFYDKKQLKLFLDELYNQPNLKIQAFFRLLAMTGMRKQEAGALEWR
                     DIDFKAKTVNIYKAVTRTANGLEIDTTKTVGSSRIISIDQGTLDKLLEWKKVALPPSD
                     EWLIFGHSSAKKPHDIMSLDTSRKWLLSIQDKMDKKQKKKLPRITVHGFRHTQASLLI
                     EMGASLKEVQFRLGHEDIQTTMNTYAHVSKLAKEQLADKFNKFIDF"
     gene            complement(1638..3410)
                     /locus_tag="ACGE70_001583"
     CDS             complement(1638..3410)
                     /locus_tag="ACGE70_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693225.1"
                     /translation="MKKKKGTQLSVDSPNWLLIITVLCFAAFIMVSPQVYLHSVILGN
                     DIMFHFNRFYETYMQIKTGNFNFFQSLYSFQSSGRIITAFYGADFAYLQGLLLIILKS
                     WFKYQLVSSFSCFFIAGASMFWLTYKCKVRTNIGLIIALLYMSSSAVSYYPIAQAFTG
                     WGAALMPLLFVPAIEALKNKERPIRPLQLAIPVTLLLSTHLLSLVIGILAILPFYVIA
                     FINTKNKLNMIFRLVESIGLTMLFSANTIIGFIDVYLSNKILSPSPISQMLSQSMSFS
                     LEGNSWGNYGLVFTAIFFAVIIYFFLNWAKTSLTSKVIVIVGAFFMLLSSKLLPWNSI
                     PHMFKFVSFFQFPQRFSVIAFVLLLLSFALILQESKLLKDVDKKYYILTLLCALFSIF
                     NVYNLMYDQSWHWNTNDPTAAGNNKSSMVEKDPQKLREAFYNKDLNIALKAIQKGTPD
                     YLPVQKNVEPSDVLKQNPYELYTNQIINNNVHFKKTVTSDSKLRLTWTNNSNEESDIQ
                     LPIIIYNHSTVTLNGKKLTPNEIKTTQIGAAIVTSSPGKNTLVIGYKPFVLFKIAFPI
                     KILSILSTIIYVIYKYKKTKIIEI"
     gene            complement(3598..4251)
                     /locus_tag="ACGE70_001584"
     CDS             complement(3598..4251)
                     /locus_tag="ACGE70_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693226.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDATSNQETKISKTKET
                     TETSSSTSKSTSKTDSSLTVTSSNQVTAEPSPTVISSSQSTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFSKYGIQ"
     gene            complement(4358..4567)
                     /locus_tag="ACGE70_001585"
     CDS             complement(4358..4567)
                     /locus_tag="ACGE70_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HFF8693227.1"
                     /translation="MPNYVVLQVVLKEKFIGKGSQNLSELENTINRQCSKGYRLHTIS
                     TTNGGSKGFGGGDRIQATLVFESLN"
     gene            complement(4644..5396)
                     /locus_tag="ACGE70_001586"
     CDS             complement(4644..5396)
                     /locus_tag="ACGE70_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFF8693228.1"
                     /translation="MKKNILGSVIKDARKKKKLTQEQLSKLTGYSQNTISNHENGNRS
                     LDEDNIKTYATALDLTPDDLFEALDIKNSLENKMMIIDKKQTKIDNLLDLYSQLEESR
                     QTKVYNFAEQQLEEQNRGNVVHFPKKEKLPTIKNSASAANPTELVYGDTVVEEEEFER
                     VPNNADFAVPIIGDSMEPVIRNGQFVFVKEQPDVEDGEIAIVEIDGDGVTCKEVYKDY
                     ESQTIILHSINELYEDRVVSPEQIRIIGKVVF"
     gene            5538..5771
                     /locus_tag="ACGE70_001587"
     CDS             5538..5771
                     /locus_tag="ACGE70_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFF8693229.1"
                     /translation="MSTMEKKKNLRVEFLKPKLKLKKARLANEWTTSYVADLIGLERR
                     QYELKEKGEYPFHDYEMYILAKNFNKKVSELFF"
     gene            5820..6581
                     /locus_tag="ACGE70_001588"
     CDS             5820..6581
                     /locus_tag="ACGE70_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HFF8693230.1"
                     /translation="MNQPQIFNFEHNEVRTFLENDVPYFVANDVAKTLGYKNPSKATN
                     DHCKKAIETWGNDSLGRRQKFKIIPESDVYRLIIKSNLPSAEKFESWVMEEVLPQIRK
                     HGMYATDELLNNPDLLIEVATKLKEERTLRLIAEQQVNELQPKADYYDRILNNKGLVT
                     VSTIAKNYGMSAVSFNKLLHELGIQFNQSGTWLLYSKFQDKGYTHIEPFDYEDKNGNR
                     QVKTRMKWTQKGHIFLYETLKKNNYLPMIEREQTA"
     gene            6712..6852
                     /locus_tag="ACGE70_001589"
     CDS             6712..6852
                     /locus_tag="ACGE70_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693231.1"
                     /translation="MEKTVSAKIEDLKLDILKQAKVAMEHAVDKEDSAMVAAIAEILA
                     HV"
     gene            complement(6849..7019)
                     /locus_tag="ACGE70_001590"
     CDS             complement(6849..7019)
                     /locus_tag="ACGE70_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693232.1"
                     /translation="MNKKIAADLVVAWLNHESQISTKGESISPKEIAQAYLDIQYAVI
                     YGQLPEDRKNDD"
     gene            7090..7290
                     /locus_tag="ACGE70_001591"
     CDS             7090..7290
                     /locus_tag="ACGE70_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693233.1"
                     /translation="MNRMDTQRIVLETEEKEFIETVISFVTEKGWTISNLQNAVSKVE
                     DYMKKNATLTELTIGMPAQIKD"
     gene            7364..7591
                     /locus_tag="ACGE70_001592"
     CDS             7364..7591
                     /locus_tag="ACGE70_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFF8693234.1"
                     /translation="MTQELINKNDLDSFLIGYVPKRYLTQKEAVHYTGTSAGTINEWV
                     KKGLKVIIFGENSRPKYDIKDIDEFMSKYKV"
     gene            7604..7777
                     /locus_tag="ACGE70_001593"
     CDS             7604..7777
                     /locus_tag="ACGE70_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693235.1"
                     /translation="MGKFNRALVFSAPLIVYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEYRAKKPTEGAD"
     gene            complement(7891..8298)
                     /locus_tag="ACGE70_001594"
     CDS             complement(7891..8298)
                     /locus_tag="ACGE70_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693236.1"
                     /translation="MSFDNTITISIILALVALISPWITAVINNKHAESMKDKEIELQK
                     HDSKTQTIQTTFSTFLNNVGICIGSNTDKNISAVKASGYAVLPYIQNEDIEVMKIFLS
                     RFGYGNTDAEQKSLETYLIDKILPILNKSLEKL"
     gene            8460..8684
                     /locus_tag="ACGE70_001595"
     CDS             8460..8684
                     /locus_tag="ACGE70_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693237.1"
                     /translation="MISVKGLGDEIFEAMMSKAQQDIKEKIISAAAQGQTSCTVRSRG
                     TTPSFLLALEDEGILNIKRRDGSVRLFWQF"
     gene            8694..8888
                     /locus_tag="ACGE70_001596"
     CDS             8694..8888
                     /locus_tag="ACGE70_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693238.1"
                     /translation="MKDFDSLGARQQPPNEASPVGVDWQENPLYPGDTCYLTEEGYVP
                     VDVILEYVQQHYPKIELGGI"
     gene            8891..9913
                     /locus_tag="ACGE70_001597"
     CDS             8891..9913
                     /locus_tag="ACGE70_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFF8693239.1"
                     /translation="MANDLTQTTQRSLDEQVISNLGRLQEQGLEMPPGYSPQNALKSA
                     FFELTNNTGENLLQMAANNQEMKTSISNALLDMVIQGLSPAKKQCYFIKYGNKVQLMR
                     SYFGTMAVLDRVTGGADITPVVVRQGDEFEVAMDGPNMVVKKHETKFENLDNEIIAAY
                     VVIKLTNGKETTTVMTKKQIDQSWAKSKMKGSGPQKEFPEEMAKRTVINRAAKTLINT
                     SNDNDLLVQAAKDTLENEFDNDRKDVTPQTEKVATLEQKFFSNKKITEPIQKEPDPIV
                     IPDDIQNEVTRVADVPAHPEIEQAHLIENEDINPIQEELIDIPDFGREEGVEDVSEFE
                     DDEYPF"
     gene            9876..10691
                     /locus_tag="ACGE70_001598"
     CDS             9876..10691
                     /locus_tag="ACGE70_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease-like domain-containing
                     protein"
                     /protein_id="HFF8693240.1"
                     /translation="MSQNLKTTSTLSDENYYSNEADWQYMSTSQYKSFLKCEAAALAK
                     LKGDWAPTSDPKALLVGNYVHSYFESKEVHEAFKEENKSKMFSSRKPYGLLKDFQIAE
                     QMIERLKQEEAFMNIYQGEKETIVTGELFGTTWKGKIDCLNVEDEYFVDIKTTKDMHE
                     RKWNENYGSRETFIVNFGYVLQMAIYQELLLQQYGKTFTPIIAAVSKQTPSEARLITI
                     DQDNINYELVMLKEKIERIVRVKNGEEKPNHCGLCEYCRGNLPITGFTSMDDL"
     gene            10707..11492
                     /locus_tag="ACGE70_001599"
     CDS             10707..11492
                     /locus_tag="ACGE70_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693241.1"
                     /translation="MTGGWIKLYRQLLEKPIWQESTPEQKVILITLLSMANHEEREWE
                     WQGEPYKASPGQFVTSLDSIRKKCGKGISLQNVRTALKRFEKYEFLTNQPTNKNRLIT
                     IVNWEVYQSKDNDLTKELTSNQQATNKQLTTNKNDKNDKNDKNDNNPRNSRKTREYAD
                     DDPNKKLAILLLKLIRKNQNIKEPDLDKWANTIRLTIESDKRTGREVQDMIVWATSND
                     FWSGVILSPTSLRKHFDKMAIQKNKRKQQNISNDELPETGEDW"
     gene            11492..12340
                     /locus_tag="ACGE70_001600"
     CDS             11492..12340
                     /locus_tag="ACGE70_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFF8693242.1"
                     /translation="MNKKLSAMAAPYGGLKTADHNCPKCGDPLYIWKTKNKDGTDRCG
                     PTCINKICGYREMVTKNQKEAIQKANEAMKKDAINRMLNSSMITDDAIWTFDFDGYKV
                     VDQETAQIKAMAQEWAKKIVNGSTIHAVITGRTGAGKTHLGAAVIKEVMRASNYKIAC
                     SFISYRELLEQLKFAMNDPEARKAVTGSLMAEIKKSDFVVIDDLGAELGRMEENNQAT
                     PYDVDVLTSLTEARLNKATIFTTNLSSKQLKHAYGERVFSRVMNGTKGNIAVFKTTTD
                     KRRNPV"
     gene            12337..12579
                     /locus_tag="ACGE70_001601"
     CDS             12337..12579
                     /locus_tag="ACGE70_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693243.1"
                     /translation="MTFVVKKMCYLDSRGRGEASVEFAKHHTTKEEADLVASVCGGEV
                     VEVIKPKRRFSKPKTKPVKKECCCPKSNQAWMRGAK"
     gene            12722..13090
                     /locus_tag="ACGE70_001602"
     CDS             12722..13090
                     /locus_tag="ACGE70_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HFF8693244.1"
                     /translation="MATRSKYGNKKHEVDGITFDSIAEAEYYDLALWQAEANGWKVKL
                     QERFELMPKFELEGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHK
                     YQVPLILAKKYRNTFKEERF"
     gene            13105..13266
                     /locus_tag="ACGE70_001603"
     CDS             13105..13266
                     /locus_tag="ACGE70_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFF8693245.1"
                     /translation="MTTEEVIQMHIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
BASE COUNT         4616 a         2177 c         2516 g         4011 t
ORIGIN      
        1 taaaaaaact tatttgaagg aacatgctta aaccataatt gtgtttttcc ttccatcagc
       61 ataaaagttc acctcaaaaa gagtgtacga acaaaagttc tttttgtaaa ccttaaaata
      121 gattttcctg aatacgtatt ggggatttct aaatgtttga atgttttttt gaatgcaaaa
      181 tcaatgcaaa aaattttgta tttctctact tttctctcct tgaccaaaat aaaaaaacgt
      241 tgatttaaca acgtttttca tacatgttta tctgtctcaa ccatacatat atggagacgg
      301 cgggagttgc ttaaatacta ttatatcaat gctcaaattt aattgaatgc cattttgaat
      361 gccacatagc tagaaatcta taaatttatt gaacttatct gctaattgtt ctttagcaag
      421 ttttgataca tgagcgtacg tgttcatagt agtttgaata tcctcgtgac ctaaacgaaa
      481 ctgtacttct ttaagcgatg ctcccatttc gatcaacaag cttgcttgag tatgtctaaa
      541 tccatgtaca gtaattctag gtagtttttt cttttgcttc ttgtccattt tgtcttgtat
      601 actcaaaagc cactttcgag aggtatcaag actcattata tcatgtggtt tttttgcact
      661 tgaatgtcca aaaatgagcc attcatcaga tggaggaagg gcaacttttt tccattcaag
      721 aagcttatct aaagtacctt gatcgattga aattattcgg ctagacccaa ccgttttagt
      781 tgtgtcaatt tctagtccat ttgcagttct agtaacggct ttatagatat tgactgtttt
      841 agccttgaaa tctatatctc tccactcaag agcgcctgct tcttgttttc gcataccagt
      901 catagctagt aatctaaaaa aagcttgaat ctttaggttt ggttgattat atagttcatc
      961 aagaaacaat ttcaattgtt ttttatcata gaatggttct tcaacagttg cttttttcct
     1021 tctctttggt ttgcgtatgg catctgtagg attggactca atcattccaa atcgaacggc
     1081 gtatttaaaa actagtcctg tgtaattcat cattttagga gctgtatcat atttatttgc
     1141 ccatttatcc attaattttt gaattttgat aggcgtgatt tcagaaatat atatgtcgcc
     1201 gagttcttct aatacatgat ttttaaaaat tctttctgtt tttaacaatg tagatcctcg
     1261 tactgttctt ttgtattcag tcatccataa atcatatacg tctttatatg tttttggttt
     1321 ttcttttgtt aataaattgt cctcgtattc actttgcaat cttgcgagtg ctaattttgc
     1381 ttcacgttga gttttaaaat ttcgccgagt agttttaaca ggctttcctg tttcggggtt
     1441 gattcctaaa tatgcttgga acttccaagc tttatccccg ttctttttct tatattgttc
     1501 aaatttagcc aattgaatca actcactttt ctggtaataa tgactaaaaa atctcagtga
     1561 tttttacaca tccattataa tattctgctg ttattaaatg gatgtgcatc acataacata
     1621 ttaatgtttt gtaattttta tatttcgatt atttttgttt ttttatactt gtagatgaca
     1681 taaatgatag ttgaaagtat agacaatatt tttattggaa aggctatttt aaataaaaca
     1741 aatggcttat aaccgataac taaagtattt tttcctggac tcgatgtaac aatagcagct
     1801 ccaatttgag tagttttaat ttcgtttgga gttaattttt taccattcaa agttactgta
     1861 ctatgattat aaattattat aggcaattga atatcggatt cctcatttga attattagtc
     1921 caagttaaac gaagtttaga atcactagtt acagtctttt taaaatgtac attgttattt
     1981 attatttggt ttgtatacaa ttcataaggg ttttgtttta atacatctga tggttctaca
     2041 tttttttgta caggtaaata gtcaggagtt cctttttgga ttgctttcaa ggcaatattc
     2101 agatctttat tataaaaggc ttcccttaac ttttgaggat ctttttcaac catagaactt
     2161 ttattgtttc ctgcagcggt gggatcattt gtattccagt gccaagattg gtcatacata
     2221 agattatata cattaaatat tgaaaataga gcacacagta aagttaatat atagtacttt
     2281 ttgtcaacat ctttaagtag ttttgattct tgcaatataa gagcaaatga taataaaagt
     2341 aaaacaaaag caatgactga gaatctttgt ggaaattgga aaaatgaaac aaatttaaac
     2401 atatgaggga tagagttcca aggtaataat ttggaagaaa gtaacataaa aaacgctcca
     2461 acaataacga ttacttttga agtaagacta gttttagccc aatttaaaaa gaaatatatt
     2521 attacagcaa agaaaattgc agtaaatact agaccataat ttccccaaga gtttccttcc
     2581 aacgagaaac tcatactttg gctcaacatt tgacttatcg gagaagggga taatatttta
     2641 ttacttagat agacatctat aaatccaatt attgtattag cagaaaatag catggttaac
     2701 cctatagatt ctactaagcg aaaaatcatg ttcagtttat tttttgtatt aataaaagca
     2761 ataacgtaaa aaggtaaaat agctaatatt ccaattacta atgaaagtaa atgagtagat
     2821 agtaataaag ttacagggat tgcaagttgc aatggacgaa taggtctttc tttatttttt
     2881 aatgcttcaa ttgctggcac aaaaagaagc ggcattaaag cagcacccca accagtaaaa
     2941 gcttgagcaa taggataata cgaaactgct gatgaagaca tgtacaacaa agcaatgatt
     3001 aatcctatgt tagttcgtac tttacactta taagttagcc aaaacatcga agcaccagct
     3061 ataaaaaagc aactgaaaga agagacaagt tgatatttaa accatgattt taagattatt
     3121 aaaagtagcc cttgcaaata agcgaaatct gcgccataaa aagcagtaat aattctgcca
     3181 gaactctgaa aagaatacag ggattgaaag aaattaaaat taccggtttt tatctgcata
     3241 taagtttcat aaaacctatt aaaatgaaac ataatatcat tccctaatat aacactatga
     3301 agatatactt ggggtgatac cattataaaa gctgcaaaac ataaaactgt aataattaat
     3361 aaccagtttg gtgaatcaac ggataattgt gtaccttttt tctttttcat tattttactc
     3421 tttcctttgt ttctttttat attttctttc tctaataact tttttactcc tcattcctat
     3481 gatattattt atatgtagga tcttagaaac gagattttag tccgtgttgc agcacgggct
     3541 tttttcttta taactttttt agagattata ggcaaaatag tagggcataa aaatatatta
     3601 ttgaattccg tatttagaaa atcctaattg aacttcaccg gaagtctttt gctgtgttgt
     3661 acgcaatgct tcttcttcag acattccatt ctgtactttc catgcaacag gcgacatccc
     3721 gtatttgtta acaaaatcag taagtgataa agtgtcagcg tcttgctgag cgcttgtttg
     3781 ttggtcttct ggattttgtt gagatgctgc ttgttgttct ttctgatctt gactgataat
     3841 attgccagca tcatctgtag tcaatccatt ttcataaagg gccacgccga aagcttccca
     3901 ctctttgttg gaccaatttg cacgatcagc tggagttgac tgtaaagtgc gttgtttcat
     3961 ctgttcatat gtttcttctt gaggtgcggt ttggattgta ctctgactgg agcttataac
     4021 tgttgggcta ggctccgccg taacttggtt ggagcttgta actgttaaac tagaatctgt
     4081 tttagatgta gatttgctag tagatgaact ggtttcagtt gtttcttttg ttttacttat
     4141 ttttgtttct tggttagaag tggcatctgt tgattcagct tttttattat ttgaacaagc
     4201 tgaaagtagc agagcagtac ttaacaacaa cataacgcta acttttttca ttttatactc
     4261 ctcttttctt tgatacaata agttgtaaag aagcctacta tataggtcta atatctcacg
     4321 tcactcaaac ttggtagggg agagggcgtg ttttttgtta atttagactt tcgaaaacta
     4381 aagtagcttg gattctatcg ccaccaccga aacctttact accaccgttt gtagtggaaa
     4441 tagtgtgtaa tcgataacct ttagaacact gtctatttat agtgttttca agttctgata
     4501 ggttttgcga cccttttcct ataaactttt cttttaatac aacttgcaat acaacataat
     4561 ttggcatatt cttttccttc tttctactat aataataaat actaacccct agccggagcg
     4621 ccaacttcga ctggggtatt tttttagaat acgactttcc cgattattct aatctgttcc
     4681 ggtgagacga ctctatcttc atataattcg tttatagagt gtagaataat cgtttgactc
     4741 tcataatcct tatatacttc tttacacgtc acgccgtcac catctatttc aacgatagca
     4801 atctcaccgt cttccacgtc tggctgttcc ttaacaaata caaattgtcc attcctgata
     4861 actggttcca ttgaatcgcc tataatagga acagcaaaat cagcattgtt tggaactctt
     4921 tcaaattctt cttcctctac aactgtatcg ccataaacta attcagtcgg gttggcggca
     4981 gaagctgaat ttttgattgt cggaagtttt tcctttttag gaaagtgcac cacgtttcct
     5041 cgattttgct cttcgagttg ctgttcagca aagttgtaga cttttgtttg gcgagactct
     5101 tccaattggc tatacaaatc taataaatta tctattttag tttgtttttt atcgataatc
     5161 atcattttgt tttctagaga atttttaata tctaacgcct caaaaagatc gtccggagtt
     5221 aaatccaaag ccgttgcata agttttgata ttatcttcat ctagggatcg gtttccgttt
     5281 tcatggttag atatagtatt ttgactgtag ccagttaatt tactaagttg ttcttgagta
     5341 agttttttct tttttcttgc gtcctttata accgaaccta aaatattttt cttcattaaa
     5401 ggcacctcct tcatgagata acaatatcac attgtgataa acgtgtaaat aaaaatatct
     5461 caaaaagtga taaaaaacta ttgacatata tctcaatatg agatattatt gatttatcaa
     5521 atgaaggagg cgattatatg agtacgatgg aaaaaaagaa aaacttaaga gttgagtttt
     5581 tgaagccgaa gttaaaactc aaaaaagcta ggcttgcaaa tgaatggact acatcttacg
     5641 tagctgattt gattggtttg gaaagacgtc aatatgagtt aaaagaaaaa ggcgagtatc
     5701 cattccatga ttatgaaatg tacattttgg caaaaaactt taacaaaaaa gtatcagaat
     5761 tattttttta aaatcaaata tctctatttg agataaaaac aagaaaggaa tgtaaaaaaa
     5821 tgaatcaacc acaaattttt aatttcgaac ataacgaagt tcgaacgttt ctggaaaatg
     5881 acgtcccata ttttgtagcg aatgacgttg caaaaacttt gggatataaa aatccaagca
     5941 aagctactaa tgatcattgc aaaaaagcga ttgaaacatg gggtaacgat tcgttaggtc
     6001 gtcgccaaaa gttcaaaatc attccagaat cagatgttta tcgcttgatt atcaaatcga
     6061 atttaccaag cgctgagaaa ttcgaatctt gggtaatgga agaagtccta cctcaaatcc
     6121 gaaaacacgg catgtacgca acagatgaac tacttaacaa tccagacttg ctaatcgaag
     6181 tagcaaccaa actcaaagaa gaacgcacgt tacgattgat agcagaacaa caagtaaatg
     6241 aattacagcc gaaggcagac tattacgacc gaatcttgaa caacaaagga ttagttacag
     6301 ttagtacgat tgctaagaac tacggtatga gcgctgtatc gtttaataaa ttgctacatg
     6361 aattaggtat ccagttcaat caaagtggca catggctgct atacagcaag ttccaagaca
     6421 aaggatacac gcatattgag ccatttgatt atgaagataa aaacggcaat cggcaagtaa
     6481 aaacaaggat gaaatggacg caaaaaggac acattttctt atatgaaaca ttgaagaaga
     6541 ataattactt accgatgatt gaacgtgaac aaacagcata ggaggtgaga tgaatggaga
     6601 gaccaaaagg gcgcgtaacc attgacctaa aaattacaaa aaccaactca aaaatgagct
     6661 ggcttcatag actattcttt tggaaagata taaaagaaag gagagaagaa tatggaaaaa
     6721 acagtctcgg caaaaatcga agatttgaaa ttagacatac tgaaacaagc aaaagtggcg
     6781 atggaacacg cggtagataa agaagactct gccatggttg cagccatagc agagatttta
     6841 gctcacgttt agtcatcgtt tttacggtct tctggaagct gaccatagat aacagcatat
     6901 tgaatatcta aataagcttg agcaatttct tttggcgaaa ttgattcgcc tttagtagat
     6961 atttgtgatt cgtgatttag ccatgcaaca actaaatccg cagcgatttt tttattcata
     7021 cttattcacc accttatgca ttatttcagt aggccactta ctgataagaa aattatatca
     7081 aagaaaggaa tgaatagaat ggacacgcaa aggattgttt tagaaacaga ggaaaaagaa
     7141 tttattgaaa cggttattag ttttgtaacc gagaaaggct ggacgatttc taatttgcaa
     7201 aatgctgtat caaaagtgga agattatatg aaaaaaaatg ccacattaac agagctgaca
     7261 attggcatgc cagctcaaat taaagattaa tagattctgc agaagcaaac aaaattatag
     7321 attcgattat tactatgtac gaattgtaga gggggaaaac gaaatgacac aagaactaat
     7381 caacaaaaat gatttagatt catttcttat aggctacgtt cctaaacgct atctgactca
     7441 aaaagaagcg gttcattata caggaacgtc agcaggaact attaacgaat gggtaaaaaa
     7501 agggttgaaa gtaatcatct tcggtgaaaa cagccgtccg aaatacgaca tcaaagatat
     7561 cgatgaattc atgtcgaaat ataaagtcta aggaggtaag cggatgggaa aatttaatag
     7621 agcattagtg ttcagcgcac ccctaatcgt ctatgcttta ggactttggg gaagcagaca
     7681 agcattaatc ggcacgatcg tttacatggt ttggattttt atggggcttg atgaagctga
     7741 gtacagagca aaaaagccaa ccgagggggc tgactaaagt gtggttctgt ttattaggtg
     7801 tttatttcgt ggccgtttta gtaaataact acatgagaaa acgtggcgaa tactggtata
     7861 catcctatgc ggttctagta tgtctaatgc ttacaatttt tctaatgatt tattcaaaat
     7921 aggtaatatt ttgtcaatca aataagtttc aagagatttt tgttctgcat cagtgttgcc
     7981 gtaaccaaaa cggcttaaga aaattttcat aacctctata tcttcatttt gaatatatgg
     8041 caggactgca taacctgatg cttttacagc agatatattt ttatcagtgt tactaccaat
     8101 acaaatgccc acattgttga gaaatgttga gaaagttgtt tgtattgttt gggtttttga
     8161 atcgtgtttt tgtaattcaa tttctttatc cttcatcgat tcagcatgct tattatttat
     8221 aaccgctgta atccaagggg atataagagc tactaaagct aggataatcg aaattgtgat
     8281 cgtgttatcg aaactcattt tttcaccacc agttttttac ctaaattata tcaaaaagga
     8341 gagaagaaaa aatgcaagaa ttagtaattt tgaaaaataa aaaaagacac gaacgccggc
     8401 aagcatgatc gtgtccaaaa gaaaaatctt ttaaaccatt ataacacagg aggaaataaa
     8461 tgatctcagt taaagggtta ggcgatgaaa tattcgaagc aatgatgagc aaagcacaac
     8521 aggacatcaa agaaaaaatc atttctgctg cagcgcaagg acaaactagc tgcactgttc
     8581 gatcgagagg aacgacacca agttttcttt tggcgctaga agatgaaggc attttgaata
     8641 tcaaacgaag agatggcagc gtcagattgt tttggcaatt ttaggaggcc gtgatgaaag
     8701 actttgattc tttaggtgcc agacagcaac caccaaatga agctagtcct gtcggagttg
     8761 attggcaaga aaatccgtta tatccaggtg atacttgcta tctaacagaa gaaggttatg
     8821 taccagtaga cgtaattcta gagtacgtac agcaacatta tccaaagatt gaattaggag
     8881 gaatttagga atggcaaatg atttaacaca aacaacacaa cgttcattag atgagcaggt
     8941 aatcagtaat cttggacggt tacaagaaca aggtttagaa atgccaccag gatatagtcc
     9001 acaaaatgcg ttaaaaagtg cttttttcga actcactaac aatacaggag aaaacttgtt
     9061 gcagatggct gcaaacaatc aagaaatgaa aacctccatt tctaatgcac ttttagacat
     9121 ggttattcaa gggttatctc cagcaaaaaa acaatgctat ttcattaaat acggaaacaa
     9181 ggttcagcta atgcgttcat attttggaac aatggcggtc cttgatcgtg taactggagg
     9241 ggcagatatt acgcctgtag tcgttcgaca aggtgatgag tttgaagttg caatggatgg
     9301 accaaacatg gttgtcaaaa agcatgaaac aaaattcgag aatttagaca atgaaatcat
     9361 tgcagcctat gtagtcatca agttaacaaa tggtaaagag accacaacag tcatgacgaa
     9421 aaagcaaatc gatcaaagtt gggctaagtc aaaaatgaaa ggttctggac cgcaaaagga
     9481 atttccagaa gaaatggcta aacggacagt aatcaatcgg gcagctaaaa cattgatcaa
     9541 cacaagcaac gacaacgatt tattagttca agctgcaaaa gatacgttag aaaatgaatt
     9601 tgacaatgat cggaaagatg taacgccaca aacagaaaaa gtggctactc tcgaacaaaa
     9661 attcttttca aacaaaaaga ttactgaacc aatacaaaaa gaaccagatc cgattgtgat
     9721 tccagatgat atccaaaatg aagtaactcg agttgctgat gtacctgccc atccagaaat
     9781 agagcaagca catttgatcg aaaatgagga tatcaatcca attcaagaag aattgataga
     9841 tattccagac tttggacgcg aggaaggtgt agaagatgtc tcagaatttg aagacgacga
     9901 gtaccctttc tgatgagaat tattactcca atgaagctga ctggcagtac atgtcaacat
     9961 cacaatataa gtccttttta aaatgtgaag ccgcagcctt agcaaagttg aaaggcgatt
    10021 gggcaccgac gtcggatccc aaagccttac tcgtaggaaa ctacgtacat tcttattttg
    10081 aatcaaaaga agtacatgaa gcgttcaaag aagaaaataa atccaaaatg ttttctagta
    10141 ggaagccgta tggattgttg aaagatttcc aaattgctga acaaatgatt gaacgactta
    10201 agcaagaaga agcattcatg aacatttatc aaggtgagaa agaaacgatc gtgactgggg
    10261 aactatttgg aacgacatgg aaaggcaaga ttgattgctt gaatgttgag gatgagtatt
    10321 ttgttgatat caaaacaacc aaagacatgc atgagcggaa atggaacgaa aactacggat
    10381 caagagaaac gttcattgtc aattttggtt acgtacttca aatggcaatc tatcaagagc
    10441 ttcttttaca gcaatacgga aagacgttta caccgatcat tgcagcagtt tctaaacaaa
    10501 caccaagtga agcaagactg atcacgattg atcaggacaa tataaattat gagctggtta
    10561 tgttaaaaga aaaaatcgaa agaattgtca gagtgaagaa cggtgaagag aagccgaacc
    10621 attgcggttt gtgcgagtac tgtagaggta atcttccaat tactggattc actagcatgg
    10681 acgatttgta gagtggtggt gatgaaatga ctggaggatg gataaagctt tatcgccaat
    10741 tactcgagaa acctatttgg caagaatcta caccagagca aaaagttatt ttaattaccc
    10801 ttctttcaat ggcgaatcat gaagaaagag agtgggaatg gcaaggcgaa ccgtacaaag
    10861 caagtccagg tcaattcgtt acttctctcg atagtattag aaaaaaatgt ggtaaaggta
    10921 tctcattgca aaatgttaga actgctctaa agaggtttga aaaatatgaa tttttaacaa
    10981 accaaccaac aaacaaaaat aggcttataa ccattgtcaa ctgggaggtt taccaatcaa
    11041 aagacaacga tctaacaaag gaactcacaa gcaaccaaca agcaactaac aagcaactaa
    11101 caactaacaa gaatgataag aatgataaga atgataagaa tgataataat cctcgcaact
    11161 ctcgaaaaac acgagagtat gcagacgacg atccaaataa aaaattggcc attcttttat
    11221 taaaactcat tcgaaaaaat caaaacatca aggaacctga tttggataaa tgggcgaata
    11281 cgattcgttt aacaattgaa tctgacaaac gaactggtag agaagttcaa gacatgatcg
    11341 tgtgggccac aagtaatgat ttctggtcag gtgtgatttt gtcaccgact agcttaagaa
    11401 agcatttcga taagatggct atccaaaaaa ataaaagaaa gcaacaaaat atttctaatg
    11461 atgagttacc agaaacaggt gaggattggt aatgaataaa aaactaagtg caatggctgc
    11521 accttacggc ggattaaaaa cagcagatca taattgccca aaatgtggtg atccattgta
    11581 tatctggaaa acaaaaaata aagatggtac tgatcgatgc ggtcctacat gtatcaataa
    11641 gatttgtggt taccgagaaa tggtaactaa aaatcaaaaa gaagctatcc aaaaagcgaa
    11701 tgaagcaatg aaaaaggacg ctatcaatcg aatgcttaac agttcaatga ttacagatga
    11761 tgccatatgg accttcgatt ttgatggata caaagtagtt gatcaggaaa cagcacaaat
    11821 aaaagcgatg gctcaagaat gggccaaaaa aattgtaaat ggtagcacga tccatgcagt
    11881 gataaccggt agaacaggag ctggaaaaac tcatctaggc gctgcagtga ttaaagaagt
    11941 catgagggca tctaattata aaattgcttg ttcatttatc agttatcgag aacttttaga
    12001 acagttgaaa tttgcaatga atgatccaga agcaagaaaa gccgtaacag gttcgctgat
    12061 ggctgagata aagaaatcag atttcgtggt aatcgacgat ttgggtgcag aactaggaag
    12121 aatggaagaa aacaatcagg caacgcctta tgatgttgat gttctcacat cgctcacaga
    12181 agctcgtcta aacaaagcta cgatattcac aaccaattta tcatcgaagc aattaaaaca
    12241 cgcgtacggt gagcgagtgt tctctcgtgt aatgaatgga acgaaaggga acatagctgt
    12301 attcaaaaca acgacagaca aaaggaggaa tccagtttga cctttgtagt gaaaaaaatg
    12361 tgctacttag atagccgagg gcgaggagaa gctagtgtcg agtttgctaa acaccacacg
    12421 actaaagaag aggctgacct tgttgcaagt gtttgtggcg gcgaagtagt cgaagttatt
    12481 aaacccaaaa gacgattttc aaaacctaag acaaagccag tgaaaaaaga gtgttgttgt
    12541 ccgaaaagta accaagcatg gatgagaggt gcaaaatgac ttgtttaaaa tgcaacgatg
    12601 aaacagttat ttggtataag acatcgcttg gatggtcaac ttgtgaacct tgtccaatat
    12661 gcaatgaaaa tggacgacgt tcgaaagaac gactcgaaag actaaagaag gagtatagca
    12721 aatggcaaca agaagcaaat acggaaacaa aaagcacgaa gtagacggca tcacttttga
    12781 ttctatcgca gaagcagagt actacgatct agccttgtgg caagcggaag cgaatggctg
    12841 gaaagtgaaa cttcaggaac gatttgagct gatgccgaaa tttgaactag aaggaaagaa
    12901 gtatcgcaag atcgagtata ttcctgactt cacattttat aaaaacggca aacttgtcaa
    12961 agtcgtagat gtcaaaggaa tgcagacaaa agactttaag atcaaggcaa agttgttttg
    13021 tcataaatat caagttccgt tgattttagc taaaaaatat cggaatacgt tcaaggaaga
    13081 gcgtttttaa cgaggtggtc catcatgaca acagaagaag tgattcaaat gcatattcga
    13141 agccttcagc gtgagattga cgatctggaa cgaacaaagg cagtgatggt caatgaaacg
    13201 gcgagaaagg caatcgattt gcacatagaa aatttaagaa gggaaattcg tagattggag
    13261 gaatgagcgt ggataagaaa gcagcaatga aacgaatcat tgaactgaca cattctgaga
//
LOCUS       DAXWOO010000072        13082 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.072, whole genome shotgun sequence.
ACCESSION   DAXWOO010000072 DAXWOO010000000
VERSION     DAXWOO010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13082
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.072"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(78..150)
                     /locus_tag="ACGE70_001604"
     tRNA            complement(78..150)
                     /locus_tag="ACGE70_001604"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(115..117),aa:Lys,seq:ctt)
     gene            531..1100
                     /locus_tag="ACGE70_001605"
     CDS             531..1100
                     /locus_tag="ACGE70_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFF8693246.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1087..1947
                     /locus_tag="ACGE70_001606"
     CDS             1087..1947
                     /locus_tag="ACGE70_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFF8693247.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2137..2841
                     /gene="yycF"
                     /locus_tag="ACGE70_001607"
     CDS             2137..2841
                     /gene="yycF"
                     /locus_tag="ACGE70_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFF8693248.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2846..4681
                     /gene="walK"
                     /locus_tag="ACGE70_001608"
     CDS             2846..4681
                     /gene="walK"
                     /locus_tag="ACGE70_001608"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFF8693249.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4678..5994
                     /gene="yycH"
                     /locus_tag="ACGE70_001609"
     CDS             4678..5994
                     /gene="yycH"
                     /locus_tag="ACGE70_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFF8693250.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5995..6864
                     /locus_tag="ACGE70_001610"
     CDS             5995..6864
                     /locus_tag="ACGE70_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFF8693251.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6919..7728
                     /locus_tag="ACGE70_001611"
     CDS             6919..7728
                     /locus_tag="ACGE70_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFF8693252.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7831..8460)
                     /locus_tag="ACGE70_001612"
     CDS             complement(7831..8460)
                     /locus_tag="ACGE70_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFF8693253.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8630..9922
                     /locus_tag="ACGE70_001613"
     CDS             8630..9922
                     /locus_tag="ACGE70_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFF8693254.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9998..10660)
                     /locus_tag="ACGE70_001614"
     CDS             complement(9998..10660)
                     /locus_tag="ACGE70_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFF8693255.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10885..11169
                     /gene="groES"
                     /locus_tag="ACGE70_001615"
     CDS             10885..11169
                     /gene="groES"
                     /locus_tag="ACGE70_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFF8693256.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11220..12845
                     /gene="groL"
                     /locus_tag="ACGE70_001616"
     CDS             11220..12845
                     /gene="groL"
                     /locus_tag="ACGE70_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFF8693257.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4343 a         2261 c         2829 g         3649 t
ORIGIN      
        1 ataaaaaaag aacagcgtaa atgttttcga aatgctttcg caccaacatt tacctgttcc
       61 gtgggtttgg cacccaatgg gccgtgaggg gctcgaaccc gcgacccgct gattaagagt
      121 cagctgctct accaactgag ctaacggccc gtacaaaaag tacttaataa tagtagcact
      181 aagaatttta aaatgcaaga ctacatttaa agaaaatcag aaaaatcttt acttccttat
      241 aggaaatcat tcatagatta ttcacatttg ctcgctatta tataagcata ccaacaaaca
      301 aagttcttgc aagagaacaa accctaacac ttttttcatt ttttactcct ttcccgccgt
      361 ctccccggcg ggtctcctgt tcttaacgat aaaagacaag aaggcttagc cttcttgttt
      421 tttgttttca tggaatcccg ctaatcaaat cacctagtta aagcaactag attgaatgtt
      481 tatcataagt ctgatatcat gtttatgaaa tgaaaaaagg agcggttatg atggaaaaaa
      541 tgcgtcagca attaatggag tgggggaagg aactagaaac attccgtctg cctcactggg
      601 aagaactccc tgacttagaa ctttatatgg accaagtcag aacgttagtt gatcgttatc
      661 tttcacctgt gatacaagga gaaaagcatc cattgcttac ctcttctatg gtaaacaatt
      721 acgttaaact aggtttgata ccagcaccag ttaaaaaaag atataacaaa gaacacgtag
      781 catttttact tgcaatcact acgttgaaac aagtattgac tattcctgaa atcaaagaag
      841 ggatattgtt ccaaggaaaa acagtaggaa ttcgtgaagc ctataatcta ttttgcgatg
      901 aacaagaagc agctgtgtgg atggtcagcc aattagcaca aggtaaatct catccgcaaa
      961 aattcgacca ggcaacccct gtagaatata ttgctgtaag agcggcaact ttatcgtttg
     1021 caatgaaact tcttgcggaa aaaacgattg ttctagaaac cgaatattta aaagaggaga
     1081 aaacaaatga aaaacaataa aatcgcaatt ttagtagatt caggtacgga tgtgccagaa
     1141 gaattgataa aaaaatacga gatgtatatg atcccattga aaattattta taaagatcag
     1201 gtatatacag ataaaattga cattacctct gaagagatct atcaacgttt gccagaggaa
     1261 atccctggta catctttgcc ggatggtgag tcgatcacag aaatctttga gcaaatcaaa
     1321 aaagacggtt atgaaaaggt acttgcggta acaatttcca gtggattgag cgggacatat
     1381 aatgtggttc gtttaattgc acaacagcaa gaagaattgg aagcatatgt actagatacg
     1441 aaaaatattg gtattggtgc aggtttttct gcgatccaag cagcaaaatg gctggaagaa
     1501 ggagtagaat gggatcaact gatcagtaat ctgaatgagt tagtcgaaag aacaaaagtt
     1561 ttctttaatg ttgcgacatt ggaatatctt caaaaaggtg gacggatcgg tttagttgct
     1621 tctattgtag gaaccgcttt aaagcttaac ccaatcatct catgtaatga agaagggatt
     1681 tattacacag taggaaaagc taggggacgt aaaaaaagtt tggataaaac ggttgcttac
     1741 gtcaaagaaa aaatcggtaa tgcaaaggtc ttcaacttag ctgttgccca tggggatgcg
     1801 aaagaagaag caattgaaat ggaagcgaga cttaaaagag agttcccaca agcaaaccaa
     1861 atctactttg gccagatttc tccggcatta gtagtccaca ctggtccggg attattgggt
     1921 gtcggtgtac aagttttaga aggataaatc aaaaaaagga aacactgagg tgaaaatcag
     1981 tgttttcctt ttttattgaa gttagaacaa aaaataataa agtattttgc actttcattt
     2041 taattttctc ttttgtttta taggcaaaga tgaaacaaaa gcagaaatac ggtataatga
     2101 gaaggtatga caataatata taagaggtgg ctctttatga aaaaagtttt agttgtcgat
     2161 gatgagaagc cgatttcgga tatcgtcaaa ttcaatttag ccaaagaagg ctatgatgtt
     2221 tatactgcat atgatgggga agaagccttg gaaaaagttg ctgaggtaga acctgatttg
     2281 attttacttg atttgatgct acctaaaatg gacggcttag aagttgctcg tgaggtacgc
     2341 aaaacttatg atatgccgat cattatggta acagccaaag attctgaaat tgataaagtc
     2401 ctaggcttag agttaggcgc cgatgattac gtgaccaagc cattttccaa ccgagaatta
     2461 gttgcacgtg tcaaagcaaa tcttcgtcgc ggtgccacgg ctgcgaagga accagaagag
     2521 gcagcacctg cggaattgac gattggtgat ttgacgatcc atccagaagc ttacatggtg
     2581 acgaaacgag gagaaacaat cgagttaaca catcgtgaat tcgaactact cttttacttg
     2641 gcgaagcacc ttggacaagt gatgacaaga gaacatttat tacaaacggt ttggggttat
     2701 gattattttg gagatgtacg aacagtggat gtaactgtac gtcgtttgcg tgaaaagatc
     2761 gaagataatc caagccatcc aaattattta gtgacacgtc gtggtgtcgg ttactatttg
     2821 cgtaacccag aacaggagta attggatgaa aagaaaagtc cgcttttttc gatcagtcaa
     2881 ttttaagatc gctattacct ttattctgat tttattgatc tcgatcgaaa ttattggtgc
     2941 ctactttata cgtggtttag aacgttccac gatcaataca tttattaaag atatgaatca
     3001 aacggtggaa tcattagcaa caacaatcag cccggaattg aatcgaaaag ataatgcaga
     3061 tgatgaagag gtaaatgcga atatcaaaag atttattgaa aacagtgcga ctagcgatat
     3121 catcgaaata cgggtcgttg acgaaaaagg gatcatccga ggaacaaccg atgtcaatga
     3181 acaaagcgct gtgggaaaga aaaatgatta tgttgatatc aatgatttca cgactaaacg
     3241 ttatgttgct ttagataacg acgataaacg ggtgaatatc aatgttcaac cgatcctgtc
     3301 cccgaccggc gatgccgtta tcggggctct ttacgtcaaa agtaatatcg aacaaaaata
     3361 ttcggaaatc aataacacag cagtcatttt ctttactgcc tcactaattg cggcatttat
     3421 ttcaatgatc gtatctgttt tagttgctcg ttcgattaca cagccgatcg gtgaaatgag
     3481 agaacaagcc attcgaatcg ctagaggaga ctacagtcgg aaagtcaaag tgcatggaca
     3541 agatgaactt ggacagctgg ctgatacctt caatcagtta gctgaacgta ttgaagaagc
     3601 tcaagataca atggaagctg aacgcaatcg tttggatagc gtgctatccc atatgacaga
     3661 cggcgtcatt gcgacagatc gcaggggtaa agtcattacg atcaatgata tggctgtttc
     3721 cttgctagat atcaaaaacg aggaagcaat cggacaatcg attttgacac ttttagatat
     3781 cgaagaagaa tatacgttgc ggaaattatt agaagatcca aacgaaatgg tacttgaccg
     3841 ttcaaccagt acattggaaa gtgatcagat gatcttgcga gtagactttt ctatgatccg
     3901 tcgcgaatca ggttttatca gcggattagt agctgtcttg catgatgtga cagaacaaga
     3961 aaaaacagaa cgggaacgtc gtgaattcgt ctctaatgtt tcccatgaac ttcgtacacc
     4021 gttgacaagt atgcgtagtt atatcgaagc attgagcgaa ggtgcgtgga aagacgagga
     4081 agtagcacca aattttttga aggtaacgct agatgaaaca gaccgaatga ttcgaatgat
     4141 caatgacctc ttaaatctat ctcgcatgga tacaggtaat acccagcttc aattagaata
     4201 tgtcaatttc aacgagttag tgaattttgt tcttgaccgg tttgatatga tggtgggaaa
     4261 tcaggaaaaa aattataaga tccgtcgtga atttacgcaa agagatcttt gggtcgaagt
     4321 agatacagat aaaatcatcc aagtagtcga taatatcatg aataacgcga tcaagtattc
     4381 accagatggg ggaacgatta cttgtcgctt attagaaacg cataataacg tggttttaag
     4441 tatcaccgat caaggattgg ggattcctaa aaaagatctg aaccgagtgt tcgagcggtt
     4501 ctatcgagta gataaagcac gtgcaagagc acaaggtggg acaggacttg gattagctat
     4561 ttctagagaa gtgattaagg cgcatcgagg cgctatttgg gcagagagca aggaaggaaa
     4621 agggtcgact ttttatatct cgctgccata tgaaccttat gaggaggaat ggtgggaatg
     4681 agaatctctg aaaaagtcgt tcgtatagga ctcattttga tggttgcgtt aagcatatac
     4741 ttctcttatg caatctggct gagcccagca ggaaaaagtt caatcaacgt agatgagaca
     4801 aacactcaaa tgatcgagtc tcaaagcaat cgaaaaactt ccgaagtctt tcttccgctt
     4861 catgttacat ggcaccattc gggaatgatc caagaaacca acagtgaaaa tctcgtgtac
     4921 cgattgcaat tgatcatcga taaagcaaga tttggaaagc tttctgaagt agtttcagga
     4981 gacaaatcac agtttgaaca ggtgaaaaat ctgaactctg gttttgagtt ggcctataat
     5041 gcgccttttt tgctaagaga atacaaggaa gcatttgatt tagatttaga tgtgtcgaac
     5101 ttggcaaaag aaagcgaaaa tatgtatttt actagaatac aattcgatca aggaaataaa
     5161 aaaatccgat ttttgaatta ttcgaaccat ctcgtatatg aagcaaatat ttcgttgaat
     5221 tggacagata tcaataaaga attaacagca aaagacacaa aatggacaga aatgacaaca
     5281 gaaccatcga cgatcgacac gcaatatgct acgaaacatt cgatcaaact gaaaaagtat
     5341 agttatatcc tgtcacagca accctataca ctttttagaa atgctttttt ccagaaacca
     5401 gatagcgtga aaaataatga tgaaagcaat gaattgattt tttacggtgg gaatgaaaca
     5461 atgtctatcc attcggaaac gcaagtagtt gattttcgtg gagagctgcc agagaaaaaa
     5521 gaaaatgcta gtgtattttc tgaaagtttc ccctatatta gcaaattagg cagttcttta
     5581 gggaacctca gatattttga ccgcagtgac aatacgatcg attatcggat attcgtggaa
     5641 ggcttcccgg tttttgggac agacagcaga ggacagattg gtgtggaagt tggcggtgaa
     5701 acgaatggtt ctgtgcaagt gaatatccaa accagtttga atacgattca agtgccgata
     5761 ccttcagacg aagaagtaga acttgcaagt acgaatgaaa tcatccagca gttaaaagct
     5821 aagggagcag atagcaacaa gattggttca gtgatcgttg gctatacatg gcaaaatgtc
     5881 aaagaaacga atcgtgtcgt tgatctctta cctgaatggt atgtgaaata taaagataac
     5941 tggtattcag ctaacgacct tctggaaaaa ttacctggat tggaggcgaa ttaattggat
     6001 ttcaaaaaaa tcgaatggat atttttcgtc gcttttttag ggttgaatgt gtttttattc
     6061 agcagttatc atgacgcggt ataccaagaa aacaatgtca ttcgctctaa tcagactgaa
     6121 tcaatccaac aacgtctggc tagcgacgat atcacttaca cggggaagat ttcgtctgag
     6181 aacaagcaag gctattatct tagcgcggag cagaccaatc ttagcacttc attagcgaat
     6241 tatcgcaagg ctggtaaaag cggcttgctg gcaagtggtg tgtttttaga cggtgatgtt
     6301 ttgacgaaat ctttatccag ttcagatgct gaaaagaacg tgatcgatcc agataaaatt
     6361 agtagttcct tggaagattt tttatctaac aaagacaata ttttattcgg taatgattat
     6421 gtgtacacga aaaacttttc tcatttagaa gaagaaccac tagaaatcca agcaagccaa
     6481 gagtatgaag gcatcccatt caatgatgat acagcaaaaa tcgttctttc tttagaaaaa
     6541 aacgataatg attatagcat cacgaaatac atgcagacgc atttatctga tatcgagcag
     6601 ctgcgcgaaa agacggaact gtatacggaa gaggaagcaa tcaatacatt gtacatcaat
     6661 aacaaaatac caagaggatc aaaaatttta tggcgtcagc tagcttattc ctgtattctg
     6721 aaagtccggg aaaaaaatgt gtatgttcct gtttggcatg tagcgatcga aacaagcgat
     6781 aaagtggtcc aaattgagag tgtcaacgct ttcagcaata cgatcgtgac aaacaatact
     6841 gtaccaaaag tggaagatca ataaaaaagc gagtataatg aaaaaagttt acaaagtaag
     6901 aagggaagta ggagtcaaat ggctgaagaa aaaccagctt ttaaaatcag tatcctagca
     6961 agtggaagtt cgggtaattc actttatatc gaaagtgaaa aaaaacggct tttggtcgat
     7021 gcaggactta gcggtaaaaa aatcacttcc ttgatggctc aaattgatcg gacaccggac
     7081 caattagatg gaatacttgt gacgcatgaa catcgagatc atatacatgg cgtgggtgtc
     7141 ttggctcgta agtatcattt ggatgttttt gccaatcagc agacttggga agcgatggaa
     7201 ccactgattg gaaatgtacc agtggaacaa aaacatctat ttgagatggg gaaagtcaga
     7261 acattcggcg atatcgatat tgagagtttc ggcgtctccc atgacgcagc ggctccgcaa
     7321 ttttatcgtt tccataaaga aaatcggtca ttcgtcatgt tgacggacac cggatattgc
     7381 agtgattata tgcgaggtgt cattgaaaat gccgatggtt atttgatgga aagtaatcat
     7441 gatcttgaga tgttacgcat gggaccttat ccgtggaatt taaaacagcg tattttagga
     7501 gatagaggac atctttccaa cgaagacggt gcacttgtca tgactgatgt gatcggtaat
     7561 cggaccaaac gaatctatct tggccatctt agcaaggaaa acaatatgaa agaacttgct
     7621 catttaacaa tggaaaatgt tttgaaagaa aaagattttg gcgttggaca tgattttgaa
     7681 atctatgata cagatccaga tgaagcaact gagctatttt cgatttaaac agttcaatca
     7741 ataaaaatat aaaaagcaga agataaaatc aatcagggtt ggttttatct tctattttta
     7801 tttttttagc atattaaaaa aataaccgta ttatcgactg actacactga tgtgctgctt
     7861 ttcatgtttc tcatccagta tctcatctac taatgcaatc gcgtaatctg ccatgctgat
     7921 cgtactgtcg ccagcagcgt ttctttgtaa ataatcatca cttaacaaat aagtacctgt
     7981 acgtttgcca tgtggatcaa ataaagaagc tggactgata taagtccaat gtagattttt
     8041 agattgtttc aattttaaca atccttcgta catattgaaa gctgttggat agaagggtgc
     8101 atttttaggt acttggtcaa tcatacgagt cgtcttttct gtatctaaga ataacgaaga
     8161 ggcaccccca acgacaatta agcgtgtatc ggttccttca agcatctcaa tcaaatggga
     8221 caaagaagtt tgatgcattt cttcttttcc tttgggagcg ttgaaagcat caacgacaac
     8281 atcaaaagat gcaagatcag tagttgtcaa ctcaaataaa tccttttgta taaacggaac
     8341 atctgatggc aatttttctg gatgacgcac gattgccgtc acgtctaaat ctctggacaa
     8401 tgcttcgtct aaaataaatg aacctgcatg gccagtagct ccaatgagtg caattttcat
     8461 aaaaaacctc cttttttatt cctgtccatt gtaagcagtt gctgaatatt cgtcaaagaa
     8521 tccgattttt gaaaagctca gtgcgcattc ctttcatggt tcgtgatcat tgggtatagt
     8581 taaattaaga aaactgattg ataaaaatag aagttgaagg agataagaaa tgaaagagtg
     8641 gttagatcgt gcctttttac caatgataac atctgttgtg aatcttagac ccttaactgc
     8701 tttgcgtaat ggaatgactt acacgatccc aataattatt gttggctcgc tgtttttctt
     8761 atttgctcat tttcctgtgg attacatcgc tcagtggata gacaaaacag gattttctat
     8821 gtattttaca caagtatacc aggctacatt tgatgtgata gccttttgga tcgtttttgc
     8881 cattgcatac gcttatgcaa aagaagaaga catcgatcgc ttaccagtcg gcataacagc
     8941 cttgtctgct tatctactgc ttattcagcc actaaatact cagatagata ctgtctggac
     9001 aggtgctgaa gggatactag gagcagttac aactgggctc atcatcggat ggggctatgc
     9061 acgtttagta agacttgcga aagggtggaa gcaaagagag aagatacctg aaaatgtttg
     9121 gcagtctttt acttcattga ttccaatcat tgttatagtc acttttacgc ttatcggttc
     9181 agcggttttt caacagttgt tcagaatgac tgttgttcaa ggagtatgga catatgtcca
     9241 gcttcccatt caatcgttca tagacacgtt gtttggtgtg cttttattag gtttttttgt
     9301 tccgttttta tggttatttg gtcttcatgg atctgcgatg gtcaatgggt tggttagtcc
     9361 cgtcttgcaa gcgaattcat tagcgaatgc agaaatctta gcctcaggac aagagttgac
     9421 ggtggcaaat ggcggacata tcgttactca gcaatttctc gatcagttca tgacggttac
     9481 gggtgctgga ttgacgttag gggctgtatt ttttatgacg gtttttgcga aatcgaagaa
     9541 atacagagaa ttaggaaatc ttgctctttt accagccttt ttcaacatca atgaatcaat
     9601 tattttttcc acaccgatcg tgatgaaccc gatgatggct gttcctttta ttttcgcacc
     9661 ggtcgtttca ggattgatta cctattccgc tttatatttt ggattcgttc ctttatttac
     9721 agcagtccaa gtcccttgga caacaccgcc gatcctctcg ggtttcttag taggcggagt
     9781 gcctgcagcg attttacaag gtatcgtgtt gtcaattagt tttttcattt atttcccatt
     9841 tttgaaaaaa atagattctg caaactgcaa aaaggaaaga cagacaaaaa atgatggagc
     9901 agcagaaaag atgaacgact aaaaaacaaa aatactcctg tcgctttaga ttgggaaaat
     9961 caccaatcta ggatgacagg agtatttata gtcatgttta tactagccct ttttggacag
    10021 ctaattgaac aagaacaact aatgtgttca ttcctacgtg tgtgatcatc gatgtccaga
    10081 tccgaccagt tgctttgtac tctaggctga agaagaaacc taggaaaaag tagattagca
    10141 gatgaccatc attatgagca aacgcaaaca gcaaagagct gatcactgct ccgacccaaa
    10201 aatttgaata tcgactgatg atccccagta tactgcggcg gaatacgaac tcttccatga
    10261 tgggtccagc aacagttgtc gccaaaataa aagcgggtgc ttccatgatc atctggatga
    10321 tattttgtgt attctcggaa ggggttgctt tgccaaacaa tacagattct aacatgacag
    10381 cgatgctttg cagaatcaat gcgacaaaga ttccaaccaa tccataaata acaatctttg
    10441 gagcagaagc tttttttacc gctgattcga tcgctaaggg ttcacgttgc ttaaaataaa
    10501 aaatagctag gacaacggcg cctagtatat acatgactgc agttgctgtg atcaattcag
    10561 acgtagattt tacaagtcct atcgaagcta ggatcaaggg tgagataaaa attagaccat
    10621 agcaaaaaat gctgaggaag ctatattttt ttaaagacat gtaattcctc cttttaaggg
    10681 ataacaagca tgcaaggcaa gcttatatcg attgatttca attatatcat aatttcaata
    10741 agcagctgtc agaataaaat ttcttttctt tgatttaaaa gcttgcaaaa aagagggaga
    10801 atgagtatta ttaaaaatgt aggttagcac tcgaaggtta agagtgctaa cagattaaaa
    10861 aaagagttat cggagggatt tatcgtgtta aaaccattag gtgatcgcgt cattatcgaa
    10921 gtcgcaaaag aagaagaaaa aactgtcgga ggaatcgttt tagcttctgc tgctaaagag
    10981 aaaccccaaa ctgggacagt tgttgcagtc ggtgaaggcc gtttgttaga aaacggtgaa
    11041 aaagttcctg ccgctgtaaa agcaggcgac caagttatgt tcgaaaaata cgccggtaca
    11101 gaagtaaaat acgaaggaaa agaatatctg atcgtcgcag gaaaagatat catggcgatt
    11161 gttgagtaaa tttggaagat aaaaaaataa tcaaaaataa tcaatgaggt gaatcaatca
    11221 tggcaaaaga attgaaattt gcagaagacg cacgcgcagc tatgttacgc ggggtagata
    11281 aattagcaga tacagtgaaa gtaacattag gacctaaagg ccgaaatgtc gtactagaaa
    11341 aatcttacgg ctctccattg atcacaaatg atggtgtgac gatcgcaaaa gaaatcgaat
    11401 tagaagatca ttttgaaaac atgggtgcaa aacttgtatc agaagtagca tcaaaaacaa
    11461 atgatattgc aggagacggt acaacgacag caactgttct gacacaagca atcgttcgcg
    11521 aaggtttaaa aaacgtaact gcaggtgcta atcctctagg cattcgtcgc gggatcgaat
    11581 tggcaacaaa agcagcagtc gaagaattgc ataatatctc aactgttgtt gattcaaaag
    11641 aagccattgc acaagtagca gctgtttctt cgggttctga taaagttgga catttgattg
    11701 ctgatgcaat ggaaaaagtt ggaaacgacg gtgtcatcac aatcgaagaa tcaaaaggga
    11761 tcgaaacaga actagatgtt gtagaaggta tgcagtttga tcgtggttac ttatcacaat
    11821 atatggtaac agataacgac aaaatggaag ctgttctaga aaacccatat atcttgatca
    11881 cagacaaaaa aatctctaac atccaagata ttctgccatt gttagaacaa atcttgcaac
    11941 aatcacgtcc attgttgatt attgctgacg atgtagatgg tgaagcattg ccaactcttg
    12001 tattgaacaa aatccgtgga acattcaacg tagtagctgt aaaagctcct ggtttcggcg
    12061 atcgtcgtaa agcaatgctt gaagatatcg ctattttgac aggtggtaca gtaattactg
    12121 acgatctagg tttagaattg aaagatgtaa caatcgaaaa cttaggaaat gcttctaaag
    12181 ttgttgtaga taaagacaat acaacgatcg tagaaggttc tggagaaaaa gaagcaatcg
    12241 aagctcgtgt gcaattgatc aaaaaccaaa ttgctgaaac aacttctgat tttgaccgcg
    12301 aaaaattaca agaacgctta gctaaattag caggtggtgt agctgttgtt aaagttggtg
    12361 cagctactga aacagaattg aaagaattaa aattacgcat tgaagatgca ttgaatgcta
    12421 cacgtgccgc agtagaagaa ggaatggtct caggtggtgg tacagctcta gtgaacgtta
    12481 tcagcaaagt ttctgctgta gaagcagaag gagacgtggc aacaggtatc aagatcgttg
    12541 ttcgtgcttt agaagaacca attcgtcaaa tcgctgaaaa cgctggttat gaaggatcag
    12601 tgattgttga taaattgaag aacgtggaac taggcactgg tttcaatgct gctactggag
    12661 aatgggtaaa catggttgaa gcaggtatcg tagacccaac taaagtaaca cgttctgcct
    12721 tgcaaaatgc cgcttccgtt tctgccttgt tattaacgac agaagcagtc gttgcagata
    12781 aaccagaacc agcagcgcca gcagctcctg caatggatcc atcaatgatg ggcggtatga
    12841 tgtaagaaaa agcaacttgc ttatctacaa ctgatcatta gtgaaagaca gataaaaaga
    12901 ggctggaaca gaagcgttta actccaagaa ataagaagaa attcacggaa attgcttttc
    12961 aaatttttgt gaatttcagc ttatttccga aggagttgct tctgcttccg ctgtttatac
    13021 gtgtttagag cgtgagacaa aagtgttctt tacttttgtc ccacgctctt ttttgtgtgt
    13081 aa
//
LOCUS       DAXWOO010000073        12989 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.073, whole genome shotgun sequence.
ACCESSION   DAXWOO010000073 DAXWOO010000000
VERSION     DAXWOO010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12989
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.073"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(<1..657)
                     /gene="cmk"
                     /locus_tag="ACGE70_001617"
     CDS             complement(<1..657)
                     /gene="cmk"
                     /locus_tag="ACGE70_001617"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFF8693258.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     L"
     gene            complement(706..1341)
                     /locus_tag="ACGE70_001618"
     CDS             complement(706..1341)
                     /locus_tag="ACGE70_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFF8693259.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(1407..2810)
                     /locus_tag="ACGE70_001619"
     CDS             complement(1407..2810)
                     /locus_tag="ACGE70_001619"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFF8693260.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(2797..3828)
                     /locus_tag="ACGE70_001620"
     CDS             complement(2797..3828)
                     /locus_tag="ACGE70_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8693261.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            3859..4080
                     /locus_tag="ACGE70_001621"
     CDS             3859..4080
                     /locus_tag="ACGE70_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFF8693262.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(4245..4982)
                     /locus_tag="ACGE70_001622"
     CDS             complement(4245..4982)
                     /locus_tag="ACGE70_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFF8693263.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(5135..6475)
                     /locus_tag="ACGE70_001623"
     CDS             complement(5135..6475)
                     /locus_tag="ACGE70_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFF8693264.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(6712..8235)
                     /locus_tag="ACGE70_001624"
     CDS             complement(6712..8235)
                     /locus_tag="ACGE70_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFF8693265.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(8613..9224)
                     /locus_tag="ACGE70_001625"
     CDS             complement(8613..9224)
                     /locus_tag="ACGE70_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFF8693266.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(9347..9472)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(9554..10270)
                     /locus_tag="ACGE70_001626"
     CDS             complement(9554..10270)
                     /locus_tag="ACGE70_001626"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFF8693267.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(10276..10845)
                     /gene="scpB"
                     /locus_tag="ACGE70_001627"
     CDS             complement(10276..10845)
                     /gene="scpB"
                     /locus_tag="ACGE70_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFF8693268.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(10829..11599)
                     /locus_tag="ACGE70_001628"
     CDS             complement(10829..11599)
                     /locus_tag="ACGE70_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFF8693269.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(11596..11967)
                     /locus_tag="ACGE70_001629"
     CDS             complement(11596..11967)
                     /locus_tag="ACGE70_001629"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8693270.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(11986..12873)
                     /gene="xerD"
                     /locus_tag="ACGE70_001630"
     CDS             complement(11986..12873)
                     /gene="xerD"
                     /locus_tag="ACGE70_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFF8693271.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         3654 a         2794 c         2226 g         4315 t
ORIGIN      
        1 caaaacaaca gcttttattg ctgcaacgac ttctggaatc gatttgccag tcgtatcgat
       61 acgaacagca tcctctgctt gcttcagcgg agagacgtcc cttgttgaat cataatggtc
      121 tctttcctgt atctctttcg tcagtgtttc aagatcagtc ggaattcctt tttcttggtt
      181 ctctttgaaa cgtctttctg cacgctcact tacgcttgct actaaaaaga ttttcacttc
      241 tgcattcggt aatacagctg tcccgatgtc tcttccgtcc atgacaacgc caccgcgttc
      301 ccctattttt cgttggacag caactaattc ttctctgact tttccatgtg cagagacttc
      361 agaaacagct tttgtgacat ctggttgacg gatttctaaa gtcacatctt ttgatgctat
      421 gaatacatgc tgtccatctt cttgttgcga aaaagagata ggatactttt tgatcagcga
      481 aactagccct tcttcatctg aaaaagatac attgttcgta atagctaaat aagttactgc
      541 gcgatacatg gcaccggtat ctgtataaat ataattcaat tcttttgcta atatcttggc
      601 aacggtactt ttcccagatg aagcaggccc gtcgattgca atactgattt gtgtcatgat
      661 ataactcctt tttctcagaa aaaaagccgc aaagcgcggc tattattatt taattcttaa
      721 ttcttgacca gggtaaagca taaagttgtt tggatctaag ccattcaatt caaacaattg
      781 atcagtggtg atccctgcac gctcagccac ttgttttgga ccttcgcctg cttgaacagt
      841 cgttgttgaa ccggctgcag ctgatgaaga aggtgttgtt tctgctgcat tgctgcttga
      901 atcccctgct tgataagttg cctcacttga ggaagtcggt gttgtctctg ctgcaatact
      961 gctttctgac gttacacctg tatcttgaga cacagtagat tcttccgcga ttgtactaga
     1021 ttctactgtt gaagtctcag ttgactcctc ttttgttgta cttgtagaac tttctactac
     1081 tgatgtcgaa gtttcagaag aagttgctgc tgttgcattg ttgcttgatc catttctgat
     1141 ccagaaataa gcacctgctg gaatagcgat acataagacc agtaaaatca aaaatagcga
     1201 aagaaataat gtatttcctt tcttttgttg tctctgagaa cttcttgatg aaacttcatc
     1261 atctgtatcg tagattggtt gttcccatgg ttcttgtgtt tcgttatttt ctgtagattg
     1321 atgtctatct tttctgctca ctgttttacc tcctaaactc tatcgtgtgt attataccat
     1381 aggaaaaagt gtcttgtcct aaaaaatcaa tgttccatgt tttttttaaa taaatttagt
     1441 aaaatcgttt cccaatgaag ttgttcagtc ttagaaaaaa gcttcctctt tggcagaagt
     1501 tctgcttttg ctccatcaat atcacaacaa ttctgcggct tttcggagag tttttcgcca
     1561 aaataagcta agatgaattt tctgcggcag ttttcttcat taatatagcg aagcatctgc
     1621 tgcaggcgga attctttttc tttttcctga cgtttgagcc gttccaccca attttctggt
     1681 ttttcagact gctggatcag ctccatccat tttttttgca gctcatcaaa gggagcttgc
     1741 tttgtggcat attccaaata aagctcaaaa ctttgtcgct gttctctaga aagttgatta
     1801 aagaaataat ggatacgttc atccccactt tgataaaaaa gaattgctgc actttcttcc
     1861 tgatctctgc ctgctcgacc gatttcttgt acatagtttt ctagactgtc tggcaaatca
     1921 taatggacca cataacggat atcagattta tcgatcccca taccaaatgc attcgtagca
     1981 atgagaaact gcagctgatt ttttacaaac tgctgttgaa gttgtcttct ttgagctgca
     2041 tcaagaccac cgtgatagta tccaactgta aaacgttccc tgaataaatg atacaatcgt
     2101 tcgacttctt tttttgttgc acaataaatg attgccgctc cttgtgctct ttccatgaag
     2161 tgttcaagtt cttgttcttt ttgctgcgtt ttccgaacaa aaagcgagat attttgccga
     2221 ttgactgatt gtctgatctc ttttggtggt cgatcaaata gtacatgacg tatatctctt
     2281 gctactaaat ctgttgctgt cgccgttagt gccagtgtga ccgggtttcc taaaagcttt
     2341 cggatttttc ctaactgttg atattctgga cggaaatcaa ctccccattg agacacgcaa
     2401 tgagcctcat ctactacata taacgcaata ttttgttttt tcagatgctc taaaagagaa
     2461 ggattagtca acatttcggg actcaaaaac aaaaatttat attgcgatag atgacggagg
     2521 acgtatcttc tttcagattg cgaaagtaaa ctattgaaag cagctactcg tttctcccct
     2581 cgtttttgta attgcgtcac ttgatcttcc attagagaaa gcagcggtga cacgatgatg
     2641 accagacctt ccattaaata ccccgtcaat tgatagcaaa ggctttttcc cgttcctgta
     2701 ggcaaaatag cgagagtatc ttctccatca agcaacgcct gaataatttc ttcttggccg
     2761 ggacgaaatg aatcaaatcc aaaatatttt tttaattcat ttattaacat tctgtatcgc
     2821 ttcctttctc aatagataga tttgatacaa acgaaattct ccataatcta attgtccatc
     2881 tacattccat tcgctaaagc gccactctcg cacggaatcc ttatttggtt ccaaacgttc
     2941 gattgtttct gcagagagaa tccactcgta tggaaaatca tcaaaaaaca aggcccactc
     3001 aataagatga tctgttactg ttcctctttt taaatgacgg attgccatga tctggtcaat
     3061 tgtctgacca ttcataaaca tctgcttggt gatcatcatg ctttgattga aattttgctg
     3121 taataatgaa tcgatcaaag cgtgccaaag cgaatctggc cgtttttcga tttgtgctaa
     3181 caaaaggtgg atacatctgc tttgttgcag ataaacctct gactcatcat tgttttttgc
     3241 cagctgataa ggaagcagcc ctgttcgctc atttccagat aattggttag ctaaaaaatc
     3301 tgctgcttct tctggtatct ccgaaaacag ctgcaccaaa ctctcatacg cagtatcaat
     3361 caaaatttca cgtggcaatt ggctttcact aagccatttt tttagttgga aagtataaaa
     3421 aggacttgtc tctgctggaa gatagtcttg aatacccgtt gccagattag aaattacttg
     3481 aacagcaaat ttaatgagcc gccaactagt ttcccctgtt ctgccatagc ggtcaaaacg
     3541 caaaccagta tgttctattt gatgttcgga caacatatca gcccctgcag gcgttagctt
     3601 tgcttcattt tcatttattt cgatccaacc ttgctgcgca attttttgca aaatttgata
     3661 aaacttatcc tgcttcaatt ctggaaaact gttatggata aacaaaagtt cgtgggtaaa
     3721 tccataaata agtactgaag tcgttcgttt tcccacaagg agttggtaaa gagaactaat
     3781 ccgcagcttg tgtcctgatg aaaatagcga taaaataaaa acaatcatat tattctcctt
     3841 aagttaggat ggttagaaat gtcactacta tgtaaacttg tacctgaacg ctgtattgca
     3901 tgcggtcttt gtcaaatcga agcgccagat attttcgatt atgatgaaga aggaatcgtt
     3961 ttatttgccc aagaaccaga ggctcatcaa cagttcgtga tgccggaaga agaaacatat
     4021 gtaaaaaaag cgtatcaaaa atgtccagtt cgagcgattt tattagaaaa taaaatataa
     4081 atataaacga aactttaaaa acgtacacta agttctctaa acttgaaaat aagccgggat
     4141 ctgtttctct tgttccgtga aatcccgact tattttgaaa gattcatttg ctatctatag
     4201 ttaattgact agttttttca cggaccctat aggacccatt tatattattt caactttttc
     4261 agccagtttt ccaatgtatt gacgatcaca gtttgctggt ctgcgttaga aatttctgcc
     4321 tctccatctc ctttttgctc accatagctg ccaaaacctc catgatttcc gccattgatt
     4381 gatacatact ctgttctttc cggtaaatac tgcttagctt cttgataagc ttcctgattc
     4441 aatactttat cttttgtcgc cgtgatcgat aaaacaggaa cagctgattc tgtcaatgtt
     4501 ccttttttgt cgggataact tgccatgaag aatacccctt ttaaatgttt gtctgaatga
     4561 ttcttattga cataacgact tgccattacg ccacctaatg aatggccacc gatcacataa
     4621 tcggaatttg gaaaatcttc tttgacctta tccgctttgt ttccgcccat cactgctaga
     4681 tcaagtggca ttttgacaat agcgactgaa aagccgtttt ttgctaaatc ttgcgcccaa
     4741 atactataac tttctggctc tacgagagca ccaggataaa aaataagtaa tggagacttt
     4801 ccatgattct ttttctctcc ttcaaaaaat aagtaatctt ctttgttttt tccttctttt
     4861 accaacactt gcgcttctga cgatggttcg tacgtatttt gctgtatata gtaataaccg
     4921 gctccaccga gtaatcctaa aatgatgact aagccaatta gtattcgtct aaatattttc
     4981 actttctccc accttttcta tctgagtgac ccctttttct taatcaagta tgattatacc
     5041 ttaaaaaaat aagagagaaa tgcaaacttt ccagcatcaa ataaaaaaag acggaatatc
     5101 catttgaata tccgcccttt ttgtatgttt atttttattt agcacataaa cgatacacag
     5161 cttctgcata aatttccatt gctttataca tactacttaa ttcccatcgt tcattcgcct
     5221 ggtgcataaa gtcaggagta tcagggaaca tcgcaccgaa tgccacacac tggttcatcg
     5281 tacgagcaaa agtagcgccg cctgatacaa acggttcagt catgtctcca gtaatatcgc
     5341 gatatgtccc caatagattt ttcactaact cactgtctag tggcacgtat aatgaatcaa
     5401 ggaagtcaaa ttcttcataa gtcaacccat atttttctgc tgttttagtt aatttagcta
     5461 ctaaatcatc tttcttgaat gtaacaggga ttctcatatc tacaccgatt tttgtttgtt
     5521 ctggtgtgat ttccaaactt gcaaagttca tcgttagctc gccggattgt tcatcttctg
     5581 ttttgcccac gacagcttca ccagttgcat tttcttgaac taattttcct aagaaattga
     5641 ttgggccaaa atcaaatact tctgaaagag caattgccag acgagaaata gcattcactc
     5701 cttgtgctgc atctttcgcg tggatacttt taccaagaac gacgatgcct tcaccttgtt
     5761 cttcgaaatc gaattcgtgt tttttcaatg cttcttttac ttcagaaagc ttttctccag
     5821 agtaaggagc tgcatcagga acaacattca acgctcctcc agcttttaca gaaaactcgt
     5881 tcgttcctgg tcctgtcaag taagcttgca ataagccttt ttctgcataa attaaaggaa
     5941 attcagcatc tggcgcaaat ccttgcgtaa ttccttcttc tttttcgtta tatttttcta
     6001 aacaacgcca taaattttct tcatctgtac caaagataaa acgaatacgt gtcttgaatt
     6061 ccacgccagc atcaatcaaa gctttgacgg catacattgc cgcaatagaa ggacctttgt
     6121 catcttggga tccgcgacca actaaccagc cgtcttttat agttggatcg aatggttttg
     6181 tttcccaatt attttcgtcc cctgctggaa caacgtccat atgacacaag ataccgaata
     6241 attcatctcc agagccaatc tctgaataac cgtaatagcc ttctggatct ttaaatgttc
     6301 taaatcctag cttttcactg atttccaata ctttatccaa agcttcaatc acttttttac
     6361 cgaagggatg tccttgtcct gaatctgctt catctagtac ggaaggaatt cggatcaatt
     6421 cgctcaatga ttctaatgct tgttcatgat gttgttctgt cacaaatttt ttcatgatat
     6481 tatcctccta atatgttaaa aagggtcatg tcaaattcct ccgacacaac ctcttcttct
     6541 caaataatga cgttttaagc ctgctccatc agcaagcaga atgttcttaa caaatgatga
     6601 aagaaaaaac actcactgaa tggagctatt gacttgtcca cgtgcttatc gatagccgtt
     6661 tatcggtttc ttcattctta tgatagacct tgtacctgta tttgtctagg tttagaacat
     6721 tgctgctaca cctaggatca cacagatggc tacaaataat atcgcaatta atttaccggt
     6781 aaatttccac cacactgaaa catcgaaacg agcaattgct acagcaccca ttacgatagc
     6841 agatgttggt gtaattaagt ttactaaacc agatgcagat tgataagcag tgattactaa
     6901 atccttaccg acgccggcaa attcacccat aggtcccatg attcccattg ttgcagcagc
     6961 taagccggaa gttgaaggaa tcaagaatga catagggata tagaaaataa atgtaataat
     7021 gatgaataca cttgaagata atgaacttaa tccttgttcg ccccaatgta agatggtgtc
     7081 tgtgattaat ccattgttca taaccacttg gataccacga gcaacagcta ctacgattgc
     7141 tacaccgatc aaatcagaag caccagctaa aaattcagag acaaattcag actctttcat
     7201 gccataagtg attgctacta cgatggacat tactaagaat aacatagtaa tttctgggaa
     7261 gtaccagtca cctaatggaa gcatgctttt acctagaacc gtacctagta ccggaaggtt
     7321 tgttaaccag tttgtcaatg attcaaaaat cgtgaaattc gcattcaagg ttgtccaagg
     7381 aatcaaactg atgatcatga taccaaaagt aataaagaac aatacattaa catgtttttg
     7441 ttttttcgtg attacttctt gacgaccgat tgcttcaata tcgaaatgct tcatatcttc
     7501 tttacgttgg ttgtatacta atgattttgt tggatctttt tcgatctttg atgcataacg
     7561 atacacatat acaatagaaa tagctaacaa aataacgaac atcaagaatc gaagaccaat
     7621 accttcaccc atcgaaatcc caacagcttg tgaagctacg cctgttgcaa aaggatttac
     7681 cgttgaagct agacagccta cttgagaccc tactaatacg attgctacag ctactactgt
     7741 atcaaagcct acagccatca tgactgggat cagtaaagca tagaatggaa tcgtttcttc
     7801 tgccattccg taagtagagc cgcctaatgc aaataacagc atcaatactg gaataagcat
     7861 tttttctttt cctttgaatt tcttcacaac tgaagcaatc ccggcatcta gtgcccctgt
     7921 tttgttgata atacctaaaa atccaccaac aaccaaaatg aagaaagaga ttgggattgc
     7981 agcaggtgtt tctttagtta gctcccccgc tggtacacct agcatcccat tgattggcgc
     8041 catgaaaata tcccacagcc cttgaggatt ttgttgtact cgttcatatg atccagcgac
     8101 gatgttgcct gcatcgtcta cactatagtg cccagctggt ataaaccaag taagtacagc
     8161 gattactgca ataataataa ataagaccgt atacgctgaa ggcatttgaa attttttctt
     8221 ttttgtttgt tccatcttct tcttcctttc ttttccgtta gagacaacaa ttttggctct
     8281 aatgttattt tatcaaaaag agtatcagaa caaaaataat aacacgcatt ttgtaaaact
     8341 tttgttcaaa aagttcgttt ttccctattc cctcctcttt tttcagtttt ttaaagcttt
     8401 cttctttttc attgtagcgt ttcctttttt ttataaacaa acattttcgt cttttaaaag
     8461 gagaagaatt tcacatttgc aaagaattga ttgattttta tgcataaatc aaaaaaatac
     8521 tcaaaacaaa caaataaaac gtttacatta ttgcatgtct tgtatttttt tccaaaaaaa
     8581 aagaccaaga aatttcttga tctttttgat atttatttga ctgctgtatg acgttgttcg
     8641 aactgatgtt gttttcgact taaccatgga agaagttgcg catgcagcac taaaaagaca
     8701 gcactcacga tgaatccttt gatcaagttg aaaggaacga tcccaatcag tacatagttt
     8761 gctaaagaca tgcccagcat ctgtgttgcg ttcagcccta aaaacattaa gtaaagaggc
     8821 gtaataacga aataatttgc gatactcata aaaatagtta gagcgatggt tcctgtaatg
     8881 acacctaaaa atttgtttct tcctttatgc tgtttcttta cactaaagaa ataataaatc
     8941 gggaatgtaa agatcgttgt tgataagaaa ctagcagtgt ctcctaccaa attatccgga
     9001 gaaaaacctg tagtcagcag atgtaatact gaacgcaaga acgctgttgc aactccggcc
     9061 gctggaccga ataagaacat gctgatcaat accgagatat cactaaaatc gatcttcaaa
     9121 aaactaaaag ccggcatgat cggaaaagca aaatactgta aaactaatgc gatcgcagca
     9181 aacatcgcta ctgcaaccat tttctgtaca cgggtgttct tcatttgaaa tcctcctgtt
     9241 aggactcctc ttcagcgaac tttttgacgt ttctctccaa atgaaaaaac tctattttcc
     9301 agggaaaata gagttagagt tatttgatca agcgtcgtca aataagctct atcttcttta
     9361 tccagactat actgtcggta tcggaatttc accgattcag ctattttttt gcaaaatagg
     9421 tcgtggacta taaccaccgg tcgggaattt caccctgccc ctgaagacga accttatata
     9481 atttttgttt acatattcat tatacatatc tattcaaaaa acgcaacatc taacaattat
     9541 aaaaagtaag tgttcatttt gagtggttca gcaaactgct gacttctttt ttgttcagtt
     9601 cacggtaatc cccaggacgt aggccttgta aagtcagatc tccgtatttt tcacgtttta
     9661 gtttttgcac aggaagacca actgcttgaa gcatattttt cacttgatga ttacgtcctt
     9721 cgtggatcgt caattccacg atactgtggt ttgtcttagg atccactgat aaaatttgga
     9781 atttagctgg agatgttttt cgtccttcga tctttattcc ttttgtcaaa ggtgcaagca
     9841 tcgattttgt agcgatccct tttacctttg caacgtaaac tttatccact tcatgttttg
     9901 gatgtgtcag gcgctgagca aaatctccat catttgtcag tagcaagatc cccgaagtat
     9961 cgtaatccag tcgacctaca ggatagatac gttcttttac catcggcaaa agatccgtaa
    10021 caacttttct tcctttgtca tctgcaacag cagaaataac tcctttaggt ttgtacaata
    10081 aatagtaacc gtattcttct tgataaattg ggacttcatc cacttccaca gtgtcatgtt
    10141 tcccaacttt tatccctaat tcccttacaa cttgtccgtt tacttttaca cgtcctgcaa
    10201 ggatgaattc ttctgctttt cgacgagaag tgatgccagc gtgggcaatt actttctgca
    10261 atctctccat tacaattatt ctcctttatt cattccatca aagaatagat ctggtgacac
    10321 gtcttcttca agtgaaattt ccatttcttg tgtatcaggc agttcttcta atgatttcaa
    10381 accaaaataa tctaagaagt agccagttgt tccatataat atcggccgac ctggtccttc
    10441 tactctacct ttttcttcaa tcagtttatg ggcagctaga cgctgtaccg ctcctgaaac
    10501 ctgaactcct cgaatctgct cgatttcagc tcttgtgatt ggctgtttat aagcgatgat
    10561 tgctaaggtt tcgacggcgg cttgagataa tgtgtttgat gtcccttgcg catatcgctt
    10621 aagtaaggga gccagctctt ttttagtcgt taatacataa tggctttccg tttcaaaaat
    10681 atgcaaagct gagtgcgagt tttgagtgta agattctttt aattctttca ctaaacttgt
    10741 gacttctgct tcgctgagat ttaacagtcc gcttaattct tcattactga tcccttcctc
    10801 gccaacaata aaaagaaggg cttcaagttc acttaatttc gtcatgttcg gtatctccta
    10861 ctgctggata aagcataatt gattcatagt tttctttttg ttgcgcaatg atctgccctt
    10921 ttttcatcaa ttctagcaaa gccataaaag tagtgacaac ttcactttta gaaggtgact
    10981 caaaaaaaga atcgaaagta cagccatgcg catttctttt tatacgctct tcgataaata
    11041 ccattcgttc ttcgatcgtt gtgtcatcgg gcgtgatcgt tttttccact ttttgagaaa
    11101 atttcttacg ttctaagatt ttctgcatgg cacggatcaa atcgattttt ttgacttgtc
    11161 ctcgcttcaa cggcttttca gcatccgcta acccatcgat gttcataggt tcttttgtgt
    11221 aataaagact tcgttcctct gctttttctg tcagtctttc agctgcatat ttatatttac
    11281 ggtattctaa aagctgagtg accaattctt ctcgaggatc ttcttcccat aattcttcat
    11341 cgtctgtgcc gaattcttgg acaggaagca atgtcttgct tttgattgcc atcaatgtag
    11401 cagccatcac taagtattct cctgcaacag ctagctcgag tgttttcatt gtatggatat
    11461 aattcatata ttgctccgtg atcgtagcga tcggtatatc atatatatcg atttccattt
    11521 gctgaatcag atgaagcagc agatcgagag gaccctcgaa tacatctaat ttaatattga
    11581 tctccgtcaa ctcattcact ctttcttctt tttctttgct agccattttc caatgtaagg
    11641 ctctgtgatt tcagttgcac ggatggagac atccgggtac ttttcctgta attcatctaa
    11701 catttgaaag cccatattct caccgcgata agacggattc aaagaaatgt catggatgat
    11761 cagctgatcg ttcgttgccc agtaaatacc aataatccct tgaatgttgg ttgaaccttc
    11821 tggcgtaaag aaaaaaagtt cataatcgtc ttcagattcg tacgtatcaa tctccttcaa
    11881 caaagaagtt ttacctgtca gtttcttatg gaaactcaat agtcccatag caattttacg
    11941 gttttctttc cggtaatgtg ttaacatctt ctcaccttcc tatgactaag cccttggaaa
    12001 atgctgctta taaacatccg tcattctttt cttcgtgata tgtgtataaa tctgagtcgt
    12061 agagatatcc gcgtgaccta acaattcctg tactgtccgc aaatcagctc cattttctag
    12121 cagatgtgtg gcaaagctat gccgcagcgt atgtggtgtt acttctttgt aaatcccagc
    12181 ctctcgtact aactgcttca gatttttcca gattccttgc cgagaaagac ctttcccatg
    12241 attattgaca aatacatgca tttcagatgc gtcctttaca agtaatggac gtgcttcgtc
    12301 taagtagcgt tccagccatt ggatcgcata atcacctaaa ggaatgatcc gctctttatc
    12361 tccttttcca actgtctgta ccagtccaag agaaagatga agatctccca atttcaatcc
    12421 aatcaattca ctgacgcgca tcccagtagc atacatgact tccaagattg cacgatctct
    12481 tatccctaac gtttttgtcg tatcaggtgc ttcaatcagc ttttctactt ctgtcagagt
    12541 caatgtactt ggtaatttct gtgctttctt cggcgtatcg atatgttgca tcggatcatg
    12601 atcagtgata cgctcttgcc gtaaaaactg atggaatctt ctgagacttg agatcatccg
    12661 agtaacagtt gctgtcgctt ttttttcttc atgcaacttc tccagaaaac ggatgactaa
    12721 aaagcgatcg acatcctgcc atgaagcaat tttctgctcc tgtagaaaat gcaagtactg
    12781 ttccaaatca cgttcatagc tttgtcgtgt attggcggac aatccccgtt caatattcaa
    12841 gtagtggaga taatcgttga tttgctcttt cataataatc cccctcatgc ctctctatca
    12901 tatcagatac cgcttcattc tgatagtctt tttccataat ctgagcaaag aaaaagccgt
    12961 ctagacactc agacggactg gtttctttt
//
LOCUS       DAXWOO010000074        12916 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.074, whole genome shotgun sequence.
ACCESSION   DAXWOO010000074 DAXWOO010000000
VERSION     DAXWOO010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12916
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.074"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            133..3660
                     /locus_tag="ACGE70_001631"
     CDS             133..3660
                     /locus_tag="ACGE70_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFF8693272.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="ACGE70_001632"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="ACGE70_001632"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFF8693273.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="ACGE70_001633"
     CDS             7448..7789
                     /locus_tag="ACGE70_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFF8693274.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="ACGE70_001634"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="ACGE70_001634"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFF8693275.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /gene="pheT"
                     /locus_tag="ACGE70_001635"
     CDS             9146..11566
                     /gene="pheT"
                     /locus_tag="ACGE70_001635"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFF8693276.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACGE70_001636"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACGE70_001636"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFF8693277.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
BASE COUNT         4386 a         2245 c         2738 g         3547 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggcaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gtgaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttaca
//
LOCUS       DAXWOO010000075        12898 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.075, whole genome shotgun sequence.
ACCESSION   DAXWOO010000075 DAXWOO010000000
VERSION     DAXWOO010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12898
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.075"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            173..2338
                     /gene="acm"
                     /locus_tag="ACGE70_001637"
     CDS             173..2338
                     /gene="acm"
                     /locus_tag="ACGE70_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFF8693278.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2610..3095)
                     /locus_tag="ACGE70_001638"
     CDS             complement(2610..3095)
                     /locus_tag="ACGE70_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFF8693279.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    3185..3530
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3575..4216
                     /locus_tag="ACGE70_001639"
     CDS             3575..4216
                     /locus_tag="ACGE70_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFF8693280.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            4235..4870
                     /locus_tag="ACGE70_001640"
     CDS             4235..4870
                     /locus_tag="ACGE70_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFF8693281.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4888..5703
                     /locus_tag="ACGE70_001641"
     CDS             4888..5703
                     /locus_tag="ACGE70_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFF8693282.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            6022..7458
                     /locus_tag="ACGE70_001642"
     CDS             6022..7458
                     /locus_tag="ACGE70_001642"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFF8693283.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7647..10094
                     /locus_tag="ACGE70_001643"
     CDS             7647..10094
                     /locus_tag="ACGE70_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFF8693284.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10324..11391
                     /gene="rlmN"
                     /locus_tag="ACGE70_001644"
     CDS             10324..11391
                     /gene="rlmN"
                     /locus_tag="ACGE70_001644"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFF8693285.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            11381..11587
                     /locus_tag="ACGE70_001645"
     CDS             11381..11587
                     /locus_tag="ACGE70_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693286.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(11584..12687)
                     /locus_tag="ACGE70_001646"
     CDS             complement(11584..12687)
                     /locus_tag="ACGE70_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFF8693287.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
BASE COUNT         4410 a         2154 c         2744 g         3590 t
ORIGIN      
        1 aaccttgtaa aaacagtttt ttggcatatt tattttttat aaatatttgt aaaataacta
       61 tcaatttttt agttaaaaga ttggaaatga gtgggggagg aattgaagaa aggatttttg
      121 agagatgata tagtaggttt tatttttagt taaaataggg gagatagaaa aaatgaaaaa
      181 gtgtagaaat attattttat caatgttatt tattattact aatataacat cactgattcc
      241 tgttcatgtc tatgcggatg caggcagaga tatcagcagt aatgtcactt cgttgaccgt
      301 ggacccaacg aatattacag atggaggaaa cataaaagta aaattctcgt ttgacgaaaa
      361 aaaacaaaat atccaaccag gtgattatct ttggatcaat tggccaagtg aaggaaatat
      421 tcgcggtgaa ggattccaaa aagaaatccc attaatgatt gaaaataaaa atgttggaac
      481 tttaacggtc agaaaagatt ctgcgcaggt tgtgtttaat gaaaatatta aaaacctaga
      541 ctcagtagaa ggttgggggc aatttgagat acaggccaga aacgtaaccg ataccggtga
      601 agaaaatacg ggaccattca ccgtgacgag cggtgataaa acagctactg taaatgtgac
      661 aaagccagct tctggttctt cttctagcgt tttttattat aaaacaggag acatgctacc
      721 tgaagatact aagcacattc gatggttttt gaatattaac aataacggca cttatgttga
      781 acaaccagtt aaaatcagtg atgaaatcca aagtggacaa agattagatc caagtacgtt
      841 tgaaattaat caaatacact taggtgaaca gaaggtttat agaggagaag agggaatcca
      901 acaattttta caagatttcc ccagcgctac atttaatttt tcagtaactg ataattacat
      961 tgaaataact ataccaaaaa actttgtcaa tcttaggaaa ataatggtga gttataagac
     1021 gatcatagaa aacccagaac aaataaattt tgagaatcat tcggaagctt ggtttaaaga
     1081 atttaataaa cctgctgttg atggagaaag tttcaatcat acagttaaaa atatttcagc
     1141 ttctggtgga gtcaacggta cagttagagg cgaattgaag attttcaaat atatcaatga
     1201 cacagagatt ggtatcccaa atgtgacttt cgaattaaga cgagcagatg aacagcctat
     1261 tcaaggacag tctagtatcc tgttgacttc gaatgaacag ggagaagcta gtatcaaagg
     1321 cctgcaagta ggagactatg ttgtaaaaga aaaagaagca cccaattgga ttgattttga
     1381 tccactaagt tcaaatgaac tcaaattctc cataaacgaa aatgatacag agggagtcag
     1441 tttacctatt tataacaaga aaaaggttac gaacatcaca gcaactaaaa tttggaacgg
     1501 tggaaccact ccaagaccga gtatttattt taaattattc agagcaagta caaacaattg
     1561 ggaaccagtt cctgatgcag aaacaaaacg attagacaat ggaataactt cagtgacttg
     1621 ggaagatatc caacagtatg atgatagtgg aaacgaatat acgtttaaag tgcaagaagt
     1681 agatcaaaac ggaaatgatt atgtcccttc aggttatcaa aaaattgaaa acgggctagt
     1741 cgttacaaat gagaataaag aagtcatttc agtctcagga caaaaaacat gggaagacaa
     1801 tgagaaccaa gacggcaaac gaccgacgac gatcacagta aatctattag ccgacggtca
     1861 accgatccaa cacaaagaag taagtgaaaa agatgattgg cgctatcgtt tcacaaatct
     1921 gccaaaatac agagacgggc aagaaatcat ctatactgta acggaagaca acgtgcccga
     1981 atacagtacg acgattgacg ggtataacat caaaaacagt tatacaccgg aaaaaacaaa
     2041 tatatctata ccaggtagcc atataacacc gtggaaaaat tttccagaga agacagagag
     2101 agagaaaatt agaaatctat tctctcaaaa attgcaaata tttgctcata caggagaatc
     2161 cagcgaacaa agaagcaatt atattagtaa gagagctgta cctaaacaga taacaaataa
     2221 atcaaaaagc attcttccta aaacaagtag taaaaaatct agttttgctg taatcattgg
     2281 tttattgata gtaactatct gtgctgggat attcttacaa aaatataaaa aagtttagtt
     2341 agaatataat attttttgtt aaatatgact gaaatcaaaa gaataaaatt ccgatttata
     2401 atcgaaaaaa gaagcatttt tatatatgct tcttttttcg tagaatcaat aaatatggat
     2461 ttggctaact atggaagaat actcgtatta gttttcagcg ttcatactag ttgttacatt
     2521 gtttgattgg ccaaaagaat ttctgatata ttttgtgcta aataaagaaa aagacgcttg
     2581 aattgggaaa taagaaaact actctatcat tacaaatttt cataaactgg cagatgttct
     2641 aagtaaacaa gtatcttttc aaagtcggaa aaatccataa tcaacgtagt ggtattgata
     2701 tttggatgaa agcctacggt atcttgacga tcaatagcag catcgatcac gacttggatt
     2761 tttttctctt tgtcgtgcat caatgctaat ggagtcactg tacctgctgg aacgcctagg
     2821 aggtcaggaa gctgttcttc agaaacaaaa gataagcgct tttcttttaa ttgttcagct
     2881 agttgtttta aatcggcgtg cttttcatca ggcaagatga caagatagat ttgttttcct
     2941 tttcttgatt tcaataatag attttttact tgcggaccag gtagggaaaa agggacattt
     3001 ttgactgacg taatcgcagg atgatcgact ttttgaaaag agatacccag tttttttagt
     3061 agttcataag cttcttgttc ggttgcacga ctcatcgcgc atcaccctcc tttttttatt
     3121 agtgtatcat aaaaaagagt tgacaaatga aaaataatca tgtaaattac acgtaaagtt
     3181 aaaacaaatg acatcgaaaa gaagagtagc cgttaggtgt ttttagagag tctctggttg
     3241 gtggaaagag gcagcagtta accgtgaaac acatcttgga gtcgattttc tgaagcgtat
     3301 agtaggaaaa tccggggagc ccgttacagc tgcggtcgtt gctagatgac cagataaggc
     3361 tggcttttcc gtgaggagtc ggtaaaaaaa ggtggaacca cgtgattaac gtcctttgat
     3421 cttcggatca aaggactttt tgttttcatc tgaacggcaa cgactgtgtg aggttttttc
     3481 tgcaaggaaa aaataaacag aggtggaacc acgtataata cgtcctcttg gcatttgcca
     3541 agaggatttt tttattttaa aggaggaaaa gaagatggag tatttgattg aagttatgcc
     3601 gcaattatta gtaggagcac tcacaacatt gaaattattt ttatttacct tgattggctc
     3661 attaccatta gggattgttt tagcattagg attgtctttg cattttaagc cgctacgcta
     3721 cttgcttcaa acatatgtat gggttatgcg gggaacaccg ctgttgttgc aactgatttt
     3781 tattttttat gggttgccga cgattgggat cgtttttgat cgtttcgaag cagcactcat
     3841 tgcctttatc ttaaactatg cagcttattt tgcagagatc tttcgtggag ggatacaatc
     3901 gattcctcaa ggtcaatatg aagcagcgca agttttgcaa ttaagtccct ttcaaacggt
     3961 gacgaaaatc gtcatcccac aagtgacaaa gattgtttta ccttctgtag ggaatgaagt
     4021 tatcaacttg gtcaaagatt cttccttgat ctatatatta ggtttaggtg acgtaatgcg
     4081 tgctgggaaa atcgcgatgg aacgcgacgc gactttactg cctttgctag gcgtggcaat
     4141 catctatctt acattgactg gtattctaac aatcgtaatg aaacaagttg aaggaaaatt
     4201 aaattattac cgctaactga taggaggaag aaatatgatt gaattgaaga aagtgaacaa
     4261 agcatttggt gaaaaaaaaa taatcaaaga tttgaatcta atcattccag atgggaaaac
     4321 attagctatc gtaggtcctt ccggtggcgg taaaaccacg cttttacgta tcttggctgg
     4381 cttagaaagt tctgacagcg gagaatttta tttagacggt gtaccgttca acccattcga
     4441 gcttaaaatg aaagaacaag ttgtaggagt cgtattccaa gacttccagc tatttccaca
     4501 tctgaatatt tttgaaaaca ttaccttagc accaaaaatg gtattgaaac agacaaaaga
     4561 agaatacacc aaaaaagcag aagaattagc ggcttcatta gggattagtg atttattgaa
     4621 tatctatcct tatcagcttt ctggcggaca aaaacagcgg gtagcgattg ccagagcatt
     4681 ggctatgaat ccacgggtat tggcttatga tgaaccaaca agtgctttag atcctgaatt
     4741 acgccagcaa gtcgagcgtt tgatcctatc attgaaagaa gatggggtaa cccaaattat
     4801 tgtcacccat gatttagtat ttgcagaaaa tgtcggtgat caattactaa aagtttcccc
     4861 aattcattaa gaaaggagaa gatagagatg aaaaaaacca gttttctatt cgctttgatc
     4921 gcaggtcttc ttctttttgc aggctgttcg aacaaaaaaa cttcagcaga taattgggcg
     4981 actatcaacg aagagaaaaa aatcgttata ggtttagacg atacatttgt tcctatggga
     5041 tttagagata aagacgggaa gctcacagga tttgatattg atttagccaa agcagtcttt
     5101 gaacaatatg ggattacggt agactttcag cccattgact ggtcaatgaa ggaatttgaa
     5161 ctgaacaatg gaacgatcga tttgatatgg aacggctatt ccaaaacgcc ggcaagagaa
     5221 aagaaagtgc aatttacaaa accttatatg gaaaacgatc aagtgctgat cacacctaaa
     5281 gaatcaggaa tcacaaactt tggacaaatg aataataaga gattaggtgc gcaaaatggg
     5341 tcatctggtt atgatgtatt tacgaaacaa ccagaagtat taaaagagat agtcaaaaac
     5401 caagatgctg ttttgtatga tagttttaat gaagcattga tcgatctgaa aagcagacgg
     5461 gtcgatggtc tgctgatgga taaagtatac gcagactatt atttgaaaca aagaaacgaa
     5521 ctagaagact tcaacataac aaaaggttct tatgagagtg aggattttgc agttggtgca
     5581 cgcaagtcag atagtacatt agtgaaaaaa atcaatgaag ccctagatca actagagaaa
     5641 acaggtgaat tccaaaaaat atccgataaa tggtttggcg aagatgttac gccgagaaat
     5701 taacaaatag gggactgtga caaaagacgt gtcacagtcc ctttttccga ataaacagtg
     5761 ttctcctgcc aactccttgt gactcggagc tgaatggcag gctcacaacc tcttggtaaa
     5821 cggtgttcaa aagccagttt ttcggtatta agccaaattt atgcaaaaat gtagaaagcc
     5881 attttcacaa aatttttctt actactcgaa actaagcgac tttttcacaa cctcctttac
     5941 atatttttat tttgttgtgg aaggggtttc gtgttattct aaaagaggaa cgattgaagg
     6001 ttataggaaa ggaagagatg aatggaacac aaagaactta cctcatttcc aaaagatttt
     6061 ctgtggggct ctgcttctgc agcgtatcaa gtagaaggag cttggcaaga agatgggaaa
     6121 ggagaatcag tctgggatcg atttgtacgg atacctggaa agacatttaa aggaacgaac
     6181 ggtgatttgg cagtagacca ctatcatcgt tataaagaag atattgcttt aatgaaagaa
     6241 caaggtctga aagcttaccg cttctctgta gcttggacac ggatttttcc taatggacga
     6301 ggagaagtca atcaagctgg gctcgctttt tatgagagat tgatcgacga attgattgaa
     6361 aacgatattg aacctatgtt gactttatat cattgggatc ttccacaagc attgcaagat
     6421 gaatataatg gttgggaatc acgtcagatc attgaagatt tcactcatta tgcagaaaca
     6481 ttatttgagg cattccgcgg gaaagtccat tactggatta gtttgaatga acaaaatatc
     6541 ttcacttcat taggttattt gttagcagca catccgccag gtgtgacaga cccgaaacga
     6601 atgtatgaag ttaaccatat tgctaattta gcaaatgcat ctgtcatcaa taaatttcac
     6661 gagttagaaa tgcctggaaa aatcggacca agctttgcgt atacgccaaa ttatccaatt
     6721 gacagtaatc ctgaaaatgt tctggctgct gaaaatgcag aagacctgat ggccaattat
     6781 tggatggatg tctatatgtg gggaaaatat ccgattgctg ccatgaggtt cttagaagaa
     6841 aaaggatggg caccaacgat tgaagctggc gatgcagaat tgctagaatc tgctaaacca
     6901 gattttcttg ggatcaatta ctatcaaacg gcgacgaatg cattcaatcc attaaacgga
     6961 gttggtgctg gaaaaatgaa tacgacagga aaaaaaggtt catcagaaga aactggagtt
     7021 cctggaatgt ataaaaaagt tgagaatcca tttgtcgaac gaacaaattg ggactgggag
     7081 attgacccag aaggattaag gatcggactg cgtcgtatca caagccgtta ccgcatgccg
     7141 gtactgatta cggaaaatgg cttaggagag tatgacaagt tgacagaaga taaacaaatc
     7201 catgacgact atcgaatcaa ttacttacaa agccacgtca aagccatcaa agaagcaatt
     7261 tcggatggag cagaagtcct aggctattgc acctggtctt atacagattt gcttagctgg
     7321 ctgaacggtt atcaaaagcg atatggattt gtatatgttg atcaagacga aacacaagaa
     7381 ggatctttgg aacgatataa gaaagacagt tattactggt accaaaaact gattgaagaa
     7441 aatggagaga acctttagtt cacatactta aaacaagaag gtacggctgg atatggccgt
     7501 gccttttctt gataaaatca tttagaaatt aagaaaaact gtaaaataag ctgaaaaatg
     7561 aaagaaagcg tcagttcttt caaacaaaac tttagttccc ttactttttt ttgtataatg
     7621 aaacttgatg aaatgaggga taataaatgg cacaaaagaa acggcctact aaaaaaagga
     7681 aaaagacgaa aaaaacgaaa aagcaaaaac agcagcagga acaaatgctg acgattggtg
     7741 ttgggtttgc cttgatttta tttgctgtct tcggcttttt aaaattaggt tttttaggaa
     7801 tattgattgc aaacggcttt cggatcattg caggaaatac ttaccaaatc ctctgtcttt
     7861 tattagccgt attaggattt tggattgtca ttaagaatac agagttttca attggtaaaa
     7921 accgacgctg gtttggcggc atactctttt atttcggtat cttattattg ttacatgccc
     7981 atttatttgg caaactccat actggggaac caaatatcat ggggacgaca tgggatttgt
     8041 tagccagtga tataaaacaa agtcaagtag acaataacgt tggtggtggg atgattggag
     8101 caatccttta tcattttact tacttcttga ttgcacagcc tggaagttat ttagcagcag
     8161 tgctattact ggcaggagga gcattcctgt tcagtaatct ggaaggctat cagctgttaa
     8221 atggtatcca atcaattgga gaacgagtac aggaattatt ggaaggtgat cctgccaagc
     8281 aagctcgaaa gcaagcggca aaggaagaga gaatgaaaca gcgcgcagaa gcgaaagaag
     8341 caaggcgctt agctgcaaaa gaagctgcag aaaaagaagc tgcagaatat gagaagaaaa
     8401 aagcggtgag ccagcaaagg gaaaatactg ctgatgaatg gcaggaacca gaaaatacag
     8461 agccggagca gctaagtttt gttccaatcg acagttttca agaaaatatc catcctgcga
     8521 atctagagaa accagtacca gatacaccaa aacagaccaa tacagcagaa ggtttttcag
     8581 atgagttgcc agaggatgac ggtacctcat tagaatttga gattgaagca gaacaggaaa
     8641 atcaagacta tgaattaccg acagttgatt tgcttgattc tataccaact gtggatcaaa
     8701 gtgatgagta taaaaaaatt gaaaaaaata tcggcgtatt agaacaaact ttccaaagtt
     8761 tcggtgtcga tgccaaggtc gtcaaagcca gcttaggacc atccgtcaca aaattcgaag
     8821 tccagccggc tgtaggggta aaagtaagta aaattgtgaa cttaacagat gatatcgcgc
     8881 ttgctttagc ggcgaaagat gtccggatgg aagcaccgat tccaggtaaa tcactgattg
     8941 gtattgaagt accaaatgga aagatcagta tggtttcctt tagagaaatc atcgaagcac
     9001 aaccaaacca cccagataaa ttactagaag tacctttagg acgtgatgtt tctggacggg
     9061 tacaaacggc tgatctttcg aagatgcctc acttactcgt ggctggttcg actggtagtg
     9121 ggaagtcagt agcaattaat gggattatca caagtatatt aatgcgtgct aagccccatg
     9181 aagtaaaact gatgatgatc gatccgaaaa tggttgaatt gaatatgtac aacggcatac
     9241 ctcatctgtt gacgccggtc gtgacgaacc ctagaaaagc agcgcaagcc ttacaaaaag
     9301 tagtacaaga aatggaagaa cgatatgaaa aattcgcggc aaccggagta agaaatattt
     9361 ctggttacaa tgaatttgtt caacagaaaa acttggaaaa cggaacgaaa catccaacct
     9421 taccattcat cgtagtgatc gtagatgaat tagctgattt gatgatggta gccagcaatg
     9481 aagttgaaga tgcgattatt cgtttagcgc aaatggctcg ggcagcgggg attcatatga
     9541 tcctagctac tcaacgtcca agtgtggatg ttatcacagg aatcatcaaa gcaaatgtac
     9601 cttccagaat ggcatttgcg gtatcaagcg gtacagattc tcggacgatc attgattcta
     9661 atggggcaga aaagctctta gggcgaggag atatgctatt cttgccaatg ggtgagaata
     9721 aaccgatccg tgtgcaagga gcattcattt ctgaccacga agtagaacgg gtcgttcaat
     9781 ttgttacaga tcagcaagaa gctcattatg aagagaaaat gatgccgaca gatgaagttg
     9841 aaacgacagg tgctccagaa cagccacaag atgagctgtt tgaagaagca aaagcacttg
     9901 tcgttgaaat gcaaacggca agtatttccc ttctccaaag aagatttcgg atcggttaca
     9961 accgagcagc acgtttagta gatgaactgg aagctcatgg agttgttggc ccgtcagaag
    10021 gaagcaagcc tcggaaagta ttcattgagc aggaagaaac atcagaaacg ttgccggacg
    10081 ataatgacat gtaaaacttg tgtgatgaag ggttgaaaaa cgaataacca ataaaaagac
    10141 aaggcatgat gataccaaaa gaaaaaatgg tttttttcca tcttttctaa tacggtatga
    10201 tgtcttgtct ttttagtttt tgtgagatta aagttctatt cgatttattt gtgaagtatt
    10261 gcattaagga aatgaatcat gtatagtatg tagcgaaact ttgagaaaaa gagggaaaat
    10321 actttggaaa aacagtccat ttatggcttg acgaatgaag aattaatcaa ttggtttata
    10381 gaaaacggcg agaaaaaatt ccgagctgcc caagtttggg aatggttgta tcaaaaacgt
    10441 gtaagtaatt tcacagaaat gacgaatcta tcgaaacaat tgatcgaaaa attatcggag
    10501 cattttatca tcaatcctct aaaacagatg gtcgtccaag aagcttcgga tggtacagta
    10561 aaatatttgt ttgaactccc tgataaaaac atgattgaga ctgtactgat gagacaagaa
    10621 tatggcttgt cagtttgtgt gacgacacaa gtaggatgta atatcggctg tacgttttgt
    10681 gcaagcgggc tgctcaaaaa gaaccgtgat ctgactgctg gagaaattgt tgcacagatc
    10741 atgatggtcc aacattattt tgatgagcgc aatcttggcg aacgcgtttc acatgtcgtt
    10801 gtaatgggga tcggtgagcc atttgataat tacgacaatg taatggactt tttgcatatc
    10861 atcaatgatg ccaaaggatt ggcaattggt gctcggcaca ttacagtttc cactagtgga
    10921 ttagcacaca aaatcaagga attcgctaat aacggcttgc aagtcaatct ggctatttcc
    10981 ttgcacgcgc cgaataatga ggtgcgaaca tcgatcatgc ggatcaaccg aagtttccca
    11041 attgagaaat tgatggaagc tgtggatgag tatttagaga aaacaaaccg tcgaatcacg
    11101 tttgaatata tcatgttgaa tcaagtaaat gatagaccag agcatgcgca acaattagca
    11161 gatctattaa aagataagaa aaaattagca tatgtcaact tgattccata taatccagtc
    11221 agtgaacatg atcaatattc tcgcagtcca aaagctgatg tattgagatt ttatgatgta
    11281 ttgaagaaaa acggcgtaaa ctgtgtgata cgtaaagaac atggtacaga tattgatgca
    11341 gcttgcggac aattgcgaag caagcaaatg aaaaaaacag ttgccaaata atcgtatcct
    11401 actcgttttt tcaattacca taaaaggagc agttttctta attaaggaac tgttcaactt
    11461 aaataagaga aaagattcta tagcgaaagc tataaagctt tttttgacaa gaaaaaccct
    11521 catcaagcaa agaaatgtcc tcaatttgat gacagcctgc ttgatggggg ttttagcaat
    11581 tatttaactg attttcttat atccttgctg gctttcttgt gtagcatgat aagtttctac
    11641 gtcaagaatg gcctgtttaa atttttctcg ggtgatttca taaggcacat gtttaatatc
    11701 atttgttgcc atggctttat ctaagattgc ttctaaatcg gtcgtgacat tgagattcat
    11761 gtcggttaac gatactggaa gttggttctt tttcataaac acatagattt tatcaaacaa
    11821 atcgaactgc tgatccatca tcaataaggt cagtacacca taactgacag atgctccgtg
    11881 aaggtatccc tccatttttt ctagaacagt cgtaccgtaa tagaatgagt gtgccatagc
    11941 agaattatag ttattgttaa ctaaaacaga aaccaatccg gtagtaacaa tgatatccga
    12001 aacgacttgt tcaattgcat atgagggacg atcgtcggca ctatctttca atgcttgcgt
    12061 accataagtc aagagacgat caatgcacat ttttccgatt tggattccta atctatccgt
    12121 gtagcctaga tcatcgccac gagcagaaaa agtagattcg acttctttgg ataaagcatc
    12181 gccaatacct gcccataagt atttatcagg tgcttcggca ataattgctg tatcaatgaa
    12241 ggtgtgtaca ggtggttttg aaggaaagaa gagtccgtcc attacgcctt cttcgttata
    12301 gatgacacat accgcagtga cgggcgaaca gttcgaacca atagtgggaa aggtgaagac
    12361 tggtttagct agtttttcac caatgacttt tacagtatcg caagcacggc caccaccgac
    12421 tgcaaacagg acgtctgctt tttggacact ttcatgctgg atcaactgtt caatatttcc
    12481 ataagttgaa ttacccccgt accagagact ggcgacaacc tctatttcct gtgtcaaagc
    12541 tacttttatt ttgttttctg cctttgaaag tgcagtcttc cctccaatca gcactacttt
    12601 tgtcccatat tctttgacga ttccatttgc tttttcatac gcatctgttc caatggtata
    12661 atttggtaag aaaacacttt gactcatatt catttccttc tttcttcgtt gaaacgggtt
    12721 aaaattttaa agagtgatcg atctgactgc ggctgtagat ggagtagagg tgaacctaag
    12781 acaggataag tgatgaataa ggtggcatga acaccttgaa atcatcaata agtatcctaa
    12841 aaacgaacaa gtacaaaaac attgaaaacg acaataatga atttttgttc ctcttagt
//
LOCUS       DAXWOO010000076        12828 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.076, whole genome shotgun sequence.
ACCESSION   DAXWOO010000076 DAXWOO010000000
VERSION     DAXWOO010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12828
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.076"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(340..702)
                     /locus_tag="ACGE70_001647"
     CDS             complement(340..702)
                     /locus_tag="ACGE70_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693288.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(776..1156)
                     /locus_tag="ACGE70_001648"
     CDS             complement(776..1156)
                     /locus_tag="ACGE70_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFF8693289.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1238..2413
                     /locus_tag="ACGE70_001649"
     CDS             1238..2413
                     /locus_tag="ACGE70_001649"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFF8693290.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2490..3074)
                     /locus_tag="ACGE70_001650"
     CDS             complement(2490..3074)
                     /locus_tag="ACGE70_001650"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFF8693291.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3275..4273
                     /locus_tag="ACGE70_001651"
     CDS             3275..4273
                     /locus_tag="ACGE70_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFF8693292.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4385..4894
                     /locus_tag="ACGE70_001652"
     CDS             4385..4894
                     /locus_tag="ACGE70_001652"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFF8693293.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4924..5583)
                     /locus_tag="ACGE70_001653"
     CDS             complement(4924..5583)
                     /locus_tag="ACGE70_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFF8693294.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5585..6349)
                     /locus_tag="ACGE70_001654"
     CDS             complement(5585..6349)
                     /locus_tag="ACGE70_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFF8693295.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6357..7034)
                     /locus_tag="ACGE70_001655"
     CDS             complement(6357..7034)
                     /locus_tag="ACGE70_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFF8693296.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7071..8456)
                     /locus_tag="ACGE70_001656"
     CDS             complement(7071..8456)
                     /locus_tag="ACGE70_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFF8693297.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8666..8750)
                     /gene="ffs"
                     /locus_tag="ACGE70_001657"
     ncRNA           complement(8666..8750)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACGE70_001657"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8773..9288)
                     /gene="tadA"
                     /locus_tag="ACGE70_001658"
     CDS             complement(8773..9288)
                     /gene="tadA"
                     /locus_tag="ACGE70_001658"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFF8693298.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9395..10252
                     /locus_tag="ACGE70_001659"
     CDS             9395..10252
                     /locus_tag="ACGE70_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8693299.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10333..10668)
                     /locus_tag="ACGE70_001660"
     CDS             complement(10333..10668)
                     /locus_tag="ACGE70_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693300.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10735..12105)
                     /locus_tag="ACGE70_001661"
     CDS             complement(10735..12105)
                     /locus_tag="ACGE70_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFF8693301.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12183..12719)
                     /locus_tag="ACGE70_001662"
     CDS             complement(12183..12719)
                     /locus_tag="ACGE70_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFF8693302.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
BASE COUNT         3828 a         2595 c         2126 g         4279 t
ORIGIN      
        1 ttttttaacg actcagttat cttatcatgt cgttagtaac atgtcaataa ctatttttta
       61 aattttttag aaatgtgata taacatccac ttcagaatca acgctgttaa atttatcatg
      121 tatttcttcc ttggtcaact ataaattaag cattactttg tgaaatgatt tttcgatcaa
      181 ttattattat tcgttataca accgttttca acatattttg aaaataaaat gcactaaaaa
      241 actatcttta gcagaacatt tcattgtatt ttttggacca aacaataaaa aaagaagttt
      301 gaaaaaaatt caaacttctt tgttcgctta atctgtattt tagaagaaag gcactccctc
      361 tacttcgtaa gttccaagaa cagagcgcat ttgacctggt tcttcccaaa tatatccttc
      421 gcctaattct tctctttgat tcttttgttc agtttcaaag acaaacaacg gaacattttc
      481 ctctttaata tgtgtatttg tcaattccat caaactaatc ttgctaacat cgatatgaac
      541 tatttctttt aagaaattaa gtatattcgc taaaccagtc tttccttcag aaagttccgc
      601 taacgcaaat tccaacttgt catcaattga atggaccaaa aattttttag taccatcctc
      661 caaatgcaac ataattgtac cagcaactct tttgcttgcc aaaatttcca cctcgatcaa
      721 cttgttctat ggcttacata aaaattctag cacattttca gaaaaaatac actttttatt
      781 tcttattttc tttccgttct agttcagata atgcttcttc tatccaaata ggtaactgct
      841 ccgcaatagt ggtaaatccg atcttacgat ttttattcgt gaaatacctt tttctgcgat
      901 atggaacagc tggatttgtt ttttctaacg ttcttaaaga taaactgatt tttttggaat
      961 attcatcaat gtcgatgatc tgaactgtta ctggctgtcc gacttttaac aatgaatgga
     1021 tatttttcgt ataaccggat tgtacttctg aaacatgaat aagtccttgc gtctcgttat
     1081 caagagaaac gaatgcgcca taaggctgta tccctgtcac ttttccttca attatttgtc
     1141 caattttata agtcatctac ttccctctct tcgcatttat cattatactg taaatagctc
     1201 aaaaaaagaa gcgacgaaaa tgactggagg tcttaagatg atcgaatttg ataaacagat
     1261 ttcaagaaaa gatacagaaa gtgtaaaatg ggacgcaatt gctgagacat atcaaatgga
     1321 tgacttactt cctttgtggg tagcagatat ggacttcttg tctcccggtg gggtagctaa
     1381 agcgtttagt gaatacatca aacatggaat ctttggttat gccactctgt ctgataaact
     1441 atatcaggct atcattcatt gggaacgcca acaccatgct gtggagttga cgaaagaaaa
     1501 tatcgttttt acaagtggtg tgttaaccag tttagctgca gcagtacaaa cttttacaaa
     1561 accaggcgat tctattttga ttcatgaccc agtttatcct ccattttcct ctattataga
     1621 aacaaatcaa cgtcacatcg ttcgaagctc tttgctagaa aaaaataatc atttcatcat
     1681 ggacttgtct gacatggaga aaaaaatcga agaaaacaat gtaaaagcaa tgattttatg
     1741 caatcctcac aatcctggag ggcgtgtttg gagcaaagaa gaattgaaac agttaagcga
     1801 attgtgttta aaacatcaag tgattctgtt tagtgacgag atccatcaag atttaacttt
     1861 attcgatcat acatttactt ccatgctaac gattgatcct aatttagatg atctactagt
     1921 tgcttttact tccgcaacca agactttcaa tctagctgca atcaaaaatt caatggtatt
     1981 tatcaaaaat ccagagatga aagctgattt tgaaaaacat ttagtgatga accaacagca
     2041 cgaaatcaac acttttggac tgattggaac ccaagcggct tatgagacgg gtggagaatg
     2101 gttaagccaa ctgattcctt atctagaaaa aaatgttcaa acagttaatc agttttttga
     2161 acagtatctt ccgaaagtga gaataatgaa ccctgaagga acttacttaa tgtggttaga
     2221 ttttaccgcc tatatttcag acgaccaaga attggagaac actcttgttc ggaaaggaaa
     2281 agtcgtatta aatcctggca tcacgtttgg tccttccggt catgggcata tgcgcttgaa
     2341 tattgcttgt ccagaagaaa ctttactgga aggacttaat cgaatcaagt atgctttaga
     2401 gacaatcgat taaatcattt attcaaaata aaaggacgag accttactag gtttcgtcct
     2461 ttttcataat tactccgtac cataccaaac tattctgaaa tttcaattgt ttcaatcact
     2521 acatcatgta caggtcggtc ttgtggacca cgttgtacat ttgcgatttc atctactgtg
     2581 tccatccctt ctagtacatg tccaaagact gtatgtcgga aatctagcca aggagttccg
     2641 ccacctttat acgcttcaat gatttctgat gggaatccag caccttctag ttgtccaagc
     2701 atattagctg gcacatttgt gttatttaca ataaagaatt ggcttccgtt tgtgtttgga
     2761 cctgaatttg ccatagataa agctccgcga aggttgaaca gttcacgact gaattcatct
     2821 tcaaaacttt cgccataaat actttcgcca cccattcctg ttccagttgg atcgccgcct
     2881 tggatcatga aatcaggaat aacgcggtga aaaatcacgc catcataata accttttttg
     2941 gaaagttcaa tgaaattttg aaccgtcttc ggtgctagtt cagggaataa ttgaacagta
     3001 atgtcaccac ggtttgtttt gataacagct tttggtcctt tttcactttt taaatctagt
     3061 tgtgggaatg ccatattgtt tcctcctaat atttgcatac ttcataataa taataacata
     3121 atacctaacc tctcgtaaaa atactagatt tgttttcttt tatttttcat cactagatac
     3181 attctataac ttaccgtttc gatttcgaat tcaaacattt ttgagaagca ttctcaatta
     3241 aattctgcta tagtgtgaaa agggaggaat ttttatggaa atctatgaca ttacaatcat
     3301 cggtgcaggt cctgtaggga tgtttgccgc tttttacgca ggaatgcgcc aagctaaaac
     3361 aaaaatcatt gacagtctac ctcaacttgg tggtcaatta gcaactttgt atcccgaaaa
     3421 atatatttac gatattcctg ggtatccagc aataaaagca agtgaattaa tcgatcaatt
     3481 agaaaaacaa ttaactactt ttaaccatac attccattta aaagaagaag ttctctcgct
     3541 tactcgtgaa gacgaagtta tcgagattac tacaaataaa ggcatccatt attccaaagc
     3601 agtcatttta gctttaggaa atggttcatt ccagccaaga aaattgaatt tagataatgc
     3661 tgaatcattt gaaaaccacg gattggatta tttcgtgaat gatttgatga agtatgctgg
     3721 aaaaaaagtc gctattgctg gaggtggtga ttctgctatc gattggacgt tgatgttaga
     3781 gcctattgca agcgaagttt acttgattca tcgtcgcccg gaatttcgtg cgcacgaaca
     3841 tagtgtatca cgcttgaaat cttcatctgt caacttgctt actccctatc tcatcgatgg
     3901 attatcaggc aataatgggg aactgacaga tatccggtta aaaaaagtga aaagtgacga
     3961 gacgattgat ttgatgattg attctctgat cgtcaattat ggattctctt ctaatttaga
     4021 acatctttct tcttggggac ttgatagtac cagaaatgcc attactgtaa aatcagatat
     4081 gtctacttct attccaggcg tttatgcagc tggtgacatc tgtacctatg aaggaaaagt
     4141 gaaactgatt gctactgggc tcggtgaagc acctactgca gtgaacaacg ctttacacta
     4201 tattaatcca aaggaacgaa ctcagcctgg tcacagcaca agcttatacg ataaaaatct
     4261 tcgtcctagt tgatttttta tatctctttg catttcaagt taaacccgaa agcagtttct
     4321 tgcatttttt tcatgttccg ttaacatgaa atagagaaag aaaaaataaa gggggagcaa
     4381 taatatggtc gtcaaaactg agactttaga agaaaaggca agagaactat tgaaggaacg
     4441 tggcgtaact gtagaagata tcgcacaact tgtcatgttt ttacaaaaag actatatttc
     4501 tgatttgact ttggcggatt gtactgaaag tgtcaacagc gtgctaacaa agcgtgaagt
     4561 acataacgcc attatcactg gaatccaatt agatatatta gcagaaaaga aaaaattgat
     4621 gagtccgttg caacatatta tcgaagatga cgaaggcctt tacggtatcg atgagatcct
     4681 agccttgtca atcgtgaatg tttacggtac gatcggcttt acgaattatg gttatatcga
     4741 caaggtgaaa cctggtattt taaaagaact aaacagccac gatggcgttc atgtccatac
     4801 cttcttagat gatatcgtcg gagcgatcgc cgctgctgct gccagtcgat tagcacacaa
     4861 agaaccagaa aaaagaagtc gccttactca ataattcaac aaaaaaagaa agaaaacgtc
     4921 tatttagacg agttctttct tttttgtttg tttcaagcta ttttttccta agaaaagaaa
     4981 aacagcaaaa aataattcta ataatacaaa aaataagata aagctatgca tgaaaaattc
     5041 ttctggaagt acattgatga ttggatcagt aacgggatcg aataaccagt catcgttgtt
     5101 gaaaaacgtt tcatgaaaaa ggataaaaaa gcgatcaaat cctatcatca tgaaaaaacc
     5161 gaaaacaact ggcagtaaca tgccgatttg aaacggacga atcaaccgcc aaagccgatc
     5221 attcttttgc aaatatttga taaacaaaat actcgggatc aaagtgatga aaaacacagc
     5281 ataatccact aaaaacaaaa ttttcacttc ataaaagtga tgggctccgc tagcagaaac
     5341 tgggaaatct ggcaagctga ggatggtttt gaagggatta ttgagataat tcattaggtg
     5401 atcaaaattt ttcagtaacg tctcttgatc taaagaagta taatccaaaa tttgcaaatg
     5461 tccgatatca aatacgtata gtggtcgaaa gttgatcgtc acaccaactg ccagactgat
     5521 cagtgtcaga aataggctga acaatccagc atattccatc cactgccatc tcgaattttt
     5581 catattagaa gctccattca tccagtgatt gtttcacata ggtagctggg ataggcaacc
     5641 caggtacatc ttcttctttt gtaaaacctg ataaaacgag caaagtatct atattattgc
     5701 gtatcccagc ttggatatct gtttcatagt tatctccaac catgattact tcttcttttt
     5761 gcagccctaa tacttcaacg gctttatcca taatgatggc ttctggtttt ccgatgtaga
     5821 tcggcggcgt ttgggttgct gtctctacaa aagagatgac tgaccctgct ccaggaagaa
     5881 ggccgcgttc agtaggtata ttcttatctg ggttcgttcc aatgaaagta gcaccttttt
     5941 gaatagcaag tgttgcttta actacttttt cataggtcag ataattatct aagcctacca
     6001 ctacatagtc aggagcctct tcttcccatg taaatccggc agctaaaatc aagtcaatca
     6061 atcctgcttc tccgataaca tagacttttt tcccttttcc atctgctttc ataaaatcga
     6121 ttgttgccaa agtagctgtg tagactgtct ctggagctac atgaatgtca aactcatctg
     6181 ctagacgatt agctactgtt tctggtgact tagtcgtgtt attggtaaca aacaagaaag
     6241 gtaattttct tttttgcaat tcttccacaa atcgtttacc ggccggtatg ggttgtgtcc
     6301 cacgataaat cgttccgtct aaatcaatta aatatccttt atagtccatt catccactac
     6361 tcttcccttt ttctgatcgt aaatgctgtt tttgtttttt ctacttgttt aggttgttct
     6421 tgttttttta ctttttcaga ggtttttgct gactctgttt ttcgattttt aataaccggt
     6481 tttttgcgat tagatgcgac tggtcctttt ttctcatcga tatacgcacg attatggttt
     6541 tttttctttt ttcggcgctg agttgtcttt tcttttttac cgccgattct ctcgatgaca
     6601 aaatacgcac aaccaaaatt gcaatattcg taaagatagt cttccaagga atcgattcgc
     6661 tgatctggat gcgcccgtcg attatccgct tggaaaaatc cttttaggcg tagttgttca
     6721 taaccccaat ctcctacgat ataatcatat cgggctagta catcactgta acgttctcca
     6781 agcttttctg cattaaatcc ttcacgataa tcggacacaa gccgataatg tctttcacct
     6841 atcaaaaaat tactgtcatc taacagtgtc actagctcgc ctttgacttt ttctgcttta
     6901 ggctcctcaa cgatttcttc caaagcagct gtcagttctt cggtgactgt tgtttcctgt
     6961 tcagctgatg attttttctt agcaatttct gttttatgct caacgttctg ctgttcattt
     7021 tgttttgcag acatgcgttc accacctatc ttttttatta taccttgttt ttatttgatt
     7081 gggtagtgag ctttcaagta atctcctacc acacttcgga taaattcagg gaacaaaaat
     7141 tcattttttc cagcaatctc aatcgttgga aaaaatggaa caaacatcaa atgatctacg
     7201 gtagtaaaag tataattttt attcagatcg attggcttgt tcttccaaaa gacttgatgg
     7261 ttaatagaat catatgtgat tccattatag accatctgcc caaaaatctt accgcgaaat
     7321 cccattccct tcataggata gttacgtaaa aagttacgat ttttttctat ttctaaaact
     7381 aagcggatca gatcatttcc acccaatgtg acatttaaca agtgcatcgg atgcggcaac
     7441 gcagtgtgaa gctgatcctg attgacccgc ccttttggaa tttcagttaa aaacagacca
     7501 ctgtttaata ttgctgcttc cgtctttcct cgttctttca ccgcctcaaa ggctacttgg
     7561 ataaacgaat gctcattgaa gatatctaga ctcagagaaa atggcaaatc tgctacttct
     7621 ttttctttta ataattcatg cccacgttct agataactat ttatttcttg ttcatcttct
     7681 gtaaaagctg tcattgtttc ggtagggatg gctttggctg aatgcttaag gatttttttc
     7741 tcttcatcga tggttaaatg gacttcaccg acatactgcc cgtactttcc agcggctgct
     7801 atttggacat cattgattac gtgaccttct ctgaataaat gatgggtgtg cgatcctaaa
     7861 ataacatcaa ttgctggtat ttcttttgca atttggaaat catcctgaat cccgagatga
     7921 ctcatcaaaa ctaaaacatc cacttgtgaa cgtaaatctt cgactaattc tggcaaaata
     7981 tcataaggat tccgtatgtc ccagccattc ggattataag taagcggaaa tggagcagta
     8041 aatgcaatta gtccgatttt cgtctggtgt ttcgttgtaa tgattttata tggtttcgtc
     8101 catgccggtg gctggagcgt atttttatcg aataagttat ccaggatgat atcaaactca
     8161 gcatgatcgt ataaatgatt caactcttct ttggaatttc ctacgccttc attgtttcct
     8221 atcgtcgctg catcataacc aacttcattc atcagcaaga tattttcctg tccattcgtt
     8281 gcttctgaca atggatgcca acgatccaca aaatcaccta gatctactgc aatcgttgtc
     8341 gttttttctt tcttttcgtt ttctcgttta cgttgatcca aaaaccttct gatccttggc
     8401 caattttcta aatgagaatg cagatcattc gtatgtaaaa tcactatttt ttccatgtac
     8461 cctctcgcct ctctttatct ttatcatacc ataggcagct ttttcttcca ttgaaacaag
     8521 ctctatttca gagtttttcc tgccttgaag caaattgtat aagtacaata ttcgataaat
     8581 cagtctggct aaaaaatcag gaacattcat ttttacagta ctataaaaat aaaaaaaaca
     8641 gacccgaaag tctgttgtcg tttcattata atcagacaca tggcggaacc tgcttagtgc
     8701 tgcttccttc cagacctgac acgcttcaca agcccaccat tgtcctagcc tttcggcaaa
     8761 gaatagtata ccttattttt cattcgttga ccagtctttt tttaatttct tttttcgctg
     8821 gcggagcttt cggaaaaagt cagtcaaaat catcccacat tcgtcttcta aaatgccacc
     8881 ttctacatat gcttggtggt taaaccgctc gtcctccaaa agattcatca aggtaccggc
     8941 tgttccgcct tttggatcat acgcgccgaa ataaacttct gctacacgag acaaaatcat
     9001 cgcgccgctg cacataggac aaggctctaa ggtaacaaaa agtgcagcat cttccaagcg
     9061 ccagcttccc actttttcac aggcttcctt gattgcaagc atttctgcat gtgtagtagc
     9121 atcttgcgtg gtttctctaa ggttatatcc tcttccaatc acttttccat ccaatacaac
     9181 cactgctcca atcggaactt catgaagcat ttctgctttc tttgcttctg ccaatgcaag
     9241 gcgcatccat ttttcttttt ctactgcatc aagatttgta tttaccaatt acccttccct
     9301 gcctttgttt tttcttgttt tactttagca tatgataaaa aggctgtaac taactttttt
     9361 ttctatggta aaataaatac aagaggtgaa caagatgaaa atggataaat tacttgctct
     9421 ttacccacaa gcacagaaaa aaaactctcc agcatcagaa gcggatatcc tttcttttgc
     9481 aattgatgat gcttttatct ggatcaaaca agattcatta tctcaacaag aaaccagcct
     9541 tttgaaagcg ttattccctg taatcaacga tcaaaaaaaa cacccatggt atcactatct
     9601 ttttaaagat gcttcttgtg ttcaagaaaa aagctttcgt ttgatccagc ttcacgtcga
     9661 atcaaaagga gaattcttaa aaaaagaatg gcaagatact attttggaat tatttcgcta
     9721 ttctgctgat ttcttttttt atacagacac agatgcgctc ttagtcgaac aaataaaccg
     9781 tgattatctt gatgctactg aaatcaacgg tatttttcta tctttagatg ctgattttga
     9841 tacgacaacg tctgtattcg ttggttcttt ccattcacct ggcaccgacc tcgtcccttt
     9901 gtttgctgaa gaaagaaaaa tttttttaac agagcaaaac aatctttata ctcacagccg
     9961 tactttttct atgcaagatg tggctttaca ttactataca aaagatactg taaaagatag
    10021 taagttgatc aaaagttaca tagaactgat caaacaaaat gaaatggaag tgatcattca
    10081 agaattatgg aatgaattag gcaatgtcag ctctgctgca aaaacactgt ttcttcatcg
    10141 taatactcta aaatatcgaa tcgaaaaatt tcaagaacag tctggtttca atctaaaaaa
    10201 agccaacgat ttactgtttt gctatctttt attgcttcat gaacataatt aaagatagat
    10261 taaaggggtt gtgacaataa ttttgtcaca accccttatt tcgaatgaat agtgttcaaa
    10321 agctagtttc tcttacatca ttttttcaac aaaatgcatt agccggtctt ttacatcttc
    10381 ggttgaatct ttgattgaat caccataaac agagattttc ttttttcctg atttgatttt
    10441 tgctaacatt cccatcaaag aatccaagtc actatctgaa agaccatcta cgatttctag
    10501 taatttttca tttccatcaa atttgtcata gacgaatttt tttactttcg tccgagtagt
    10561 catatgatgg atcttttcca ttacttttcc cgatacaacg actgctgtcg ctacactggc
    10621 agcggctgct acgcttaatc cgattgttac ttttgttgat gttttcataa gtatcagctc
    10681 ctttttatct atctccatca tagcatagac aaaaaaataa acgaactaat ccactcagtg
    10741 aaatctcaat cctttaggga aaaagttctt tactgttcct tttacttgtt tcccaaatcc
    10801 gacaggtatc ttatctaata ctagcaaaac ccatccttga ttcccatcac gttgaaatgt
    10861 ttctcctgct gtgtaagatt gccattcctt ttgagtgatc ggcaagcaag gtatattctc
    10921 tatctttttt gtagcaagcg ctaatgcata gctaggttca aatcgattct ttttgaaatc
    10981 tcctaaatgc aaacctgtac gtacgacttt taaaccatcg agagaagggg ctaactcagg
    11041 aacttcccaa agatggtcgt tgaacactaa aagtcttcca gtagcttcat aatgaaaatc
    11101 atttgaaaac tccgtccaga gtttttcctg ctcttttgtc atttgcactt tacttttttt
    11161 tcgtgtcttc ttttctttat gcatctgatt ttgtccatga aaagtcagct ttgcaacaaa
    11221 atgtccttca ccctgatctt tatgaggcca tatacgtatt gttttttcca aaccagctac
    11281 gcttccccat tcagaacgtc ctgaactaac ggattgagtc aaaggaatct cctcaatcgt
    11341 tactggataa ttttctacaa gccagctaat gatctcttca ttttcttctg gtgcaaacgt
    11401 acaagtcgaa taaatcagtt gtcctttatt ttttagcatt tttattgctg aagacaaaat
    11461 ctcttgctga cgtttttgac agtatagcgg agattcttct gtccactctt ttattgcatt
    11521 tggatctttt cggaacattc cttctcctga acaaggagca tcaaccacga tccgatcaaa
    11581 aaagcccgaa aaatgaggga caagttctgc gggtgcatga tttgtcacta ttgcgttcga
    11641 aactccccat cgttctatat tttctgataa aatcttcgca cgtttcggaa agatctcatt
    11701 cgtcactaat aaccctttcc ccttcatttg agcagctaat tgggttgatt taccaccggg
    11761 agccgcacac aaatccaaaa ctttttcacc gggttttgct gcagcagctg ttccaacgat
    11821 cattgcactt ggttcttggc tatattcata cccagcttga tggaggaaag actttccatt
    11881 gacagtgcca agaaatccct cattgctgta gggagcgggt tgtaattctt cgctatggta
    11941 agtttggacc atgtccaatc ctgccggttt tagtggattc caccgaaatc cttttttcac
    12001 gcttccttgt tcaagcgcag aaaaaaaatc ggatgcttct tcgcctaaaa gaagacgata
    12061 ttttttgata aattgctgtg gtaacgttgt cgcttcctcc ttcattccaa tgtttcgtta
    12121 gacattttac aatctttttt acgaaaaata aaggtggctg atctactttt tcatgttgct
    12181 gcctaaggtg actgttcctt taagatattt tccaagtatt catccgtcac ctcgttttta
    12241 tcccaaacag cgttcatcat ttcaagaaaa tcttgagaag aatcatctga gcgcaaactt
    12301 cccaatgcat taagccatac caattcaagc gacttttctt ctttgatctt cgtgtgacct
    12361 ttgacatatg agaaaaatgg ccgcttaaat agcgaatcca tgtctaaatc agattttgca
    12421 atgatttcac gaatactttc atggatagaa aaaaaatctt tcaataagta gacaatgctt
    12481 aaaacatcat cggaactgca ttcagctaag tagttttcaa aataaacagt gatttcttct
    12541 aatatcttct cttttaaccc gctgattgat ttgaaatgtg tataaatcgg gtatgtagaa
    12601 caaccgcaga actctgctaa ctctcttgca gtaattgact tcattccata ttcttccacg
    12661 tataccatac ttttgaacag caaatattct ttactataat ttgttactct ccccatcatt
    12721 cttacctccg tctcttttca tctacccata aataaataac ataaataatt ctaaaaatat
    12781 tattgataat tatcaaaaca tatttagtta tatccattaa aaatcaat
//
LOCUS       DAXWOO010000077        12716 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.077, whole genome shotgun sequence.
ACCESSION   DAXWOO010000077 DAXWOO010000000
VERSION     DAXWOO010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12716
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.077"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(<1..333)
                     /locus_tag="ACGE70_001663"
     CDS             complement(<1..333)
                     /locus_tag="ACGE70_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFF8693303.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEE"
     gene            complement(330..983)
                     /locus_tag="ACGE70_001664"
     CDS             complement(330..983)
                     /locus_tag="ACGE70_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFF8693304.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            1271..1791
                     /locus_tag="ACGE70_001665"
                     /pseudo
     CDS             1271..1791
                     /locus_tag="ACGE70_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(1789..2508)
                     /locus_tag="ACGE70_001666"
                     /pseudo
     CDS             complement(1789..2508)
                     /locus_tag="ACGE70_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010745868.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
     gene            complement(2459..2668)
                     /locus_tag="ACGE70_001667"
     CDS             complement(2459..2668)
                     /locus_tag="ACGE70_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693305.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            complement(2650..2718)
                     /locus_tag="ACGE70_001668"
                     /pseudo
     CDS             complement(2650..2718)
                     /locus_tag="ACGE70_001668"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
     gene            2945..4218
                     /locus_tag="ACGE70_001669"
                     /pseudo
     CDS             2945..4218
                     /locus_tag="ACGE70_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(4325..5356)
                     /locus_tag="ACGE70_001670"
     CDS             complement(4325..5356)
                     /locus_tag="ACGE70_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8693306.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(5363..6193)
                     /locus_tag="ACGE70_001671"
     CDS             complement(5363..6193)
                     /locus_tag="ACGE70_001671"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8693307.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(6190..7005)
                     /locus_tag="ACGE70_001672"
     CDS             complement(6190..7005)
                     /locus_tag="ACGE70_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8693308.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(7002..7826)
                     /locus_tag="ACGE70_001673"
     CDS             complement(7002..7826)
                     /locus_tag="ACGE70_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HFF8693309.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(7816..8916)
                     /locus_tag="ACGE70_001674"
     CDS             complement(7816..8916)
                     /locus_tag="ACGE70_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFF8693310.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            9094..9879
                     /locus_tag="ACGE70_001675"
     CDS             9094..9879
                     /locus_tag="ACGE70_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFF8693311.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            9912..10295
                     /locus_tag="ACGE70_001676"
     CDS             9912..10295
                     /locus_tag="ACGE70_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFF8693312.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            10371..10502
                     /locus_tag="ACGE70_001677"
     CDS             10371..10502
                     /locus_tag="ACGE70_001677"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFF8693313.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            10471..10695
                     /locus_tag="ACGE70_001678"
     CDS             10471..10695
                     /locus_tag="ACGE70_001678"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFF8693314.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFAGEVHRGLY
                     HFNGALALGDHGPHTNGNQFFYCAKHKGAG"
     gene            10670..10909
                     /locus_tag="ACGE70_001679"
     CDS             10670..10909
                     /locus_tag="ACGE70_001679"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFF8693315.1"
                     /translation="MQNTKAQADLLPRLLLPQNVKQAYLELGGLPELDGRYTVFGQVY
                     QGLEIVAAIAAQPTNSEDVPIEPVFIRQINFEKTS"
     gene            complement(10808..11779)
                     /locus_tag="ACGE70_001680"
     CDS             complement(10808..11779)
                     /locus_tag="ACGE70_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFF8693316.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            12431..>12716
                     /locus_tag="ACGE70_001681"
     CDS             12431..>12716
                     /locus_tag="ACGE70_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008177227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFF8693317.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGY"
BASE COUNT         3807 a         2852 c         2307 g         3750 t
ORIGIN      
        1 ttcttcaatt atcaaagaat tactatcact ttctaaatta acatttatat tatctttgat
       61 ttgtatttca ttcttattat ttttttcttt tatgaatact tttatttcac tactcttgga
      121 ataaaactcc aaagtttgtt ctatatattt tatttctttt ccattcatat tactagaaat
      181 ttcatttgcc atattatgaa tcttttcttt tctcgtctcc aaatatgttt taggaaagat
      241 aaaaaaaatt aatgaatgaa ctaatataat tattattcca agaacagaaa atatttgtat
      301 gaacattttt ggaaatatct ttaatttttt cattttctct ccaatttata tcctacattt
      361 cttatagtag ttatacagtc taattgtaat ttttttctaa gttcttttat atatacatct
      421 attactcgat cataaggagt ttcttcgcta tctttccata cataatcaat aatttgcatt
      481 cttgttaaca cttgtccatt attatccaat aaacatttta atacttctag ttcttttgca
      541 tttacatcta tttcttcacc atttatttta gctgtatagc tattaaaatt aaccgataga
      601 tccttatatt caaacttctc taaatgtcca taattcttct taattaaaga atctattcta
      661 gcttttaaga ccggcaatga aaatggcttt tctacatatc cgtctactaa attagtaaat
      721 gcatcaattt tatattcttc atcactaaat gcagtcaaga ttagaattgg taaattgctt
      781 ttctttctaa tttctttcaa cacttctaaa ccatttataa aaggtatctg aatgtctaag
      841 ataaccaaat ttatatcgct attaaatttt gacaaggctt ctcttccatc tttagcttga
      901 ataacagtat atccaaattc tgaaagatat tcacttattc cctctcttat catattatca
      961 tcttcaacag ttaatatttt catatatacc tccacttaag cctatacact agatattata
     1021 cagcatatta tggctctata atatctagtg ttggtgacgc cttctgttgc caatactttt
     1081 tctttttttt aataaatgca aacagacacc ttgaaatcct ttagaaaaaa ggcttctata
     1141 atattttgtg ttgttggaaa atcgaaggcg attttctact aacacttttt cttcgtttca
     1201 actcaaaaag agactcactt ttggtaaaat ttagtcgacc aaaacatcac cctaccaaag
     1261 gagtcgctct atggataatc atactagaaa attactcgga ttaacagata aacatttatt
     1321 ttttgatgaa gaatggctga ttgaaaaaga atataaaggc gttcaagcgc agtttattaa
     1381 agggagattg gatgactcac cttcccattg tgaacactgt ggatcgattc gtattatccg
     1441 caacggttcc tatacgacac gtactcagat gttgaaagtc aaagaaaagt tgactatttt
     1501 agaattaaaa cgtacccgtt ttctatgcta tgattgtggg cagacctttt ctgcgaaaac
     1561 ggacttagtt gatgaacatc atcaactaac aaaggaactg aagcaggcga ttctcataga
     1621 actgtacgaa aatcaatcac gtaatctgaa aatcagaaga agtaccgtcg gctcaagcga
     1681 tattggaaac tcttactcaa agattccacg acattggagc cgctcaaacg gcattatcat
     1741 cgactgttta aacgaccaat tagtcaaaca gaaatcgtcg atgaaccgtt gtgcacaatg
     1801 gtgtcctgaa cgtacagcga caccttccat atccagagca gtcgccacat cacggggatg
     1861 gatgccatcc aaattaaaag caaccacgcc agtatgatgt tcaggatctt gcggtccgta
     1921 aatggtgata ccgccgatcg cttgtaattt aggtaaaacg taggcaacca gttcttgctc
     1981 ataacgatga atattctcca taccaatctc attcaagtaa tcgattgctc ctgcaatgtt
     2041 cggagtacca gcttcaaatt tccatggcag ctctttccat gaactatcgt aaagattgac
     2101 aaaatctatc atttcaccgc cgaattcgac tggctccatt tgttctaaca attgacgttt
     2161 gccatataat acaccgattc ctgtcggccc gcacatttta tgcccgctaa aggcgaaaaa
     2221 atctgcatcc aacgcttgta catcaactgt catatgcggt gctgcttggg caccgactgg
     2281 actatcctca actacaacag cctgttcaac cctcagccgt ttcaagcaca acaaataacc
     2341 ttctggatct ggtttgccga cctgaagatc atcgatcgtc acaaaggaat caattttttc
     2401 cttaatcatc ggatgtgctt caaaaaaata gtctagctcc cgcagttcag aattgagact
     2461 actccgattt tccagtgctg ctgatccgct gctcaaaatg ttcagcatcc actggtctcc
     2521 caaccgcttg acttatccct tcataaaagg acagttctgc tgccgtccca acaccttgtt
     2581 gatagtctgc aaaaggaatt ttatagccga actgctccag taaataggct tcagtaaata
     2641 gtgatgaata ctctcgtaaa acatccattc actgtcaatc atcgtcccat caaaatcaaa
     2701 aataagtgct ttggtcatct tctttccccc ttacccaaaa caagcaaaag ctgtgtcctg
     2761 ctctctctac agtatcagca taaaaactgc cgtctgaaaa gtcaaaggtg aaagaaatgt
     2821 ggcgctataa tttctaggac tgatagtaaa tcattctatc agtccttttg agcataaaaa
     2881 aacaaaacat acagaagctc cgttagaatt gaatcaccac aacacaatca aaggagcgtt
     2941 ctgtatgcct gatcttcatc ttatcaaact attactagga atcaaagatg gaaacattca
     3001 aatcactcat gtcgagaaga aaaaaataca ttcaatcaac agtatcgttg tttctgccac
     3061 gctcattaaa acgattcagc cctgtcctca gtgtaaacag gtgaatcaac atgggctgat
     3121 cgttaagaac ggaaagaaaa aatcgctggt ccttctcaat aagtgtgcga atcaacggac
     3181 ttatcttgct ttggcaaagc aacggtacct ctgtcgtggc tgtcacacct attttacggc
     3241 gaatacctac attgttgaaa aaaactgttt tatcgccaaa caagtacatt ataaaatatt
     3301 ggaggaattg actgaaaaac aagcaatgac gacgatttct aaacattgcg gcgtttcttg
     3361 gtcaaccgtt tcgcgaacac tcgcttctct gctccccatg acaaacgtga aaataaattg
     3421 gttgcctcgc tgttcattgg tagatgaatt tcgttcattg aaaaatcaag tcggtcccta
     3481 ttctttttct tgtatggatg gcgatactgg gaaattactg gatattcttc cttcacgtaa
     3541 gaagaaagat ctcatttctt attttatgca atttgaacga cgtgcacgtt taaacgtaaa
     3601 aatactcgta acagatatga acgcctctta tgctttgctg ataaaagagt gctttcctca
     3661 tgccaaactc gttgtggatc gctttcacat cgtcaagcac ctcattcgca gttttaagga
     3721 tatttgacta tgagtcatga aaagcttcga ccgaaacgat ccaatacaag ccaaacacta
     3781 tcgacaagtg aaggctttat ctcgacttct gatcaaacga caggacacgc ttgtttatga
     3841 taaatggaca aaatggcgta attttggttg ggcctattta accgaaagtg aagtcgtaga
     3901 acgtctactt tctacttctg atgaactacg aatagcttat gcttactatc aagaaattct
     3961 tcaagctttt tatgacaaag aagcggacat cttctttcag cttgtaaaaa caatgcccaa
     4021 gtcggttcca agagaactgc atcatataaa aaaagcgttt ataaactatg aatccggcat
     4081 tcgtttagcc ttagagttgc cttactcaaa tggcaaaata gaaaatctgc atacccacat
     4141 caaagcattg aaacgaatag cttacgggtt aggatgagaa ccagaatttt cttaatcaat
     4201 catctgatca cttattaaac ataaaaaaac atctaaagca gaaaatcaat tctaccttag
     4261 atgttcttat atcactctat cagtcctatt tgacaaagag ccagaaatgt ttgccgacta
     4321 tacactagct ttgttgtgca aaatgctgag tatcaatttc cacagggatt gctagaatat
     4381 ctttcagctg aatgtataag gcatcgctaa ttttctcttc ccgatcgttc aaccgatcca
     4441 ctaaaatttc tttgccctcc agcgtttgaa aaacataccg ttgaatgttg cctaaaataa
     4501 atgattcctt ccatagaacc ggaatcttaa ccccctgagg aaccggttca aattgaatca
     4561 gctccggtct taaataataa atgcagcgat tgcccttagc ggaaaactcg tgggtatact
     4621 gattcagctc ataccattcg aaacgattat agttgccgat aaattccgca acaaatcggc
     4681 tctgaggatt ctgatacaat tccttcgagg tggaaacctg agccacccgc ccagcttcca
     4741 tcacaaaaat ccgatcactg attcgcatcg cctcttcttg gtcatgagtc acaaaaatca
     4801 tcgtcatccc tagcgattgc tggatagcgc gcaaatcccg ctgcagctgt tttctaattc
     4861 gggcatccaa agcacttaat ggctcatcca gcagcaggac tttcggttca gtgaccaatg
     4921 agcgagccag tgccacccgt tgtcgttgac cgccagaaag aaactggaca tttttatctg
     4981 cctgctccgt catatccacc aatgatagca tctcaaaaac cttttcctga atcacctcag
     5041 gactcagctt ctttaccttc aaaccaaagg ctacattttc aaaaacagtc atagttggaa
     5101 acaatgcata ctgttgaaaa acaaagccga tattgcgatc cttcgtaggc ttatcggcaa
     5161 tattctcatt gtccagcaca atctcaccgc tctttggttg ttccaatccc gtgatacagc
     5221 gcagcaacgt agactttccg cacccgctgg gacctagtaa agtaaccagc tcaccctttt
     5281 caatctgaat ttcatcaata gctaaaatct gtttttcatc aaaaaacaga ttgagatcat
     5341 gtatttctaa aaacattttc cgtcacaact cctttaaggg cttctttttc gactgcttct
     5401 cttccctcgt ctgcttaagg gacagctgtt gcttttgtcg ataagtcagc ataaaggtag
     5461 ttaaagcaat tcccaccata aacagcaaat acaaaaccat cacagcgcta gccaaatggc
     5521 cgttttcgtt catccgccgc agcatataga tgcgtaccgt ctgaaatcga ccgccaatca
     5581 acaggttcgt cagcatgtac tccccaaaaa ccgaagaaaa aatcatcaga gaaactaggg
     5641 tcacaccgat acggatgttt ggaaacagca cctggataat aatcgtagac atcggggcgc
     5701 ccaaggtttc tgctgcatcc actaattcct tcagattaat taagcgcatc tggttgttaa
     5761 gactaagata gacaatcggc aacgccgtaa taaacaatgc acctaccaac actacaaaca
     5821 ttgggatacc agtttcagca tacgttcgaa gcaaagccgt taccaaaatc actcctggta
     5881 aagcatacgg cagcaggagc agcttttcca gccaacgatt caaacgaggg aaatacaaat
     5941 gaatccaaat aatggtggga atcatcaaca acagaatcgt caccaagacc acacctgcta
     6001 ataaaagtga gcgacccacc gcagcaataa attccctgtc cgtcagcagc tgttggaacc
     6061 attgcaaggt aaaatcacta ggtaaaagcg attttgacca tttagtggag ctggcataca
     6121 aaatcgttcc aataatcggc agcaagtaat acaggacaaa gcagaacaaa atcggtttta
     6181 ttaacttttt cacgtctgca ccaccttgcc tttcttttga ataatcaaca ttgccgttac
     6241 cagcaggaca cctaaagcga ctgccaacgc actgcctaaa ttaggcttgg cgaacacatc
     6301 tcccgacact aaagccgcaa ttcgaatcgt taaaagattt acattactgc tggtcagggc
     6361 atacgctgtt tcataggtcc ccatgccatt agcaaataag atgacaaagg tgctggcaat
     6421 cgatggtcgc aaaaaaggca aagccacctt gaaccaagca tacgctgatg aagcgcccag
     6481 caattgtgcg gactccagcc attccttttt cacttccttc atgctgggat acaaaaacaa
     6541 gacccctaac ggaatttgga aataggtata cgtgatggtc aatccccacc aagaatacaa
     6601 attgaagtcc gctagaaatg gcaaaatcat tttcagcaca cctacattcc ccaacaaaat
     6661 aataaaagag aatgccagcg gaatcccagc aaaattagct gccaaattgg aaacaaaggt
     6721 gattttctcc tgcaatttag tcgatagatt cattaagcat aaacataaaa taaacgtccc
     6781 gattaaccca atcacactgg agctgacagc aatcagtacg ctgttaacaa aggcctgtgt
     6841 gtaatatttg ttgctgaaaa tatcactgta attggccaat gtccactggt tagttaaatc
     6901 actgtgcaag ctgcacagaa ccataaaaag cagcggcagc agtaaaaatg cacagaagat
     6961 aaaggaggca ggaaccagaa ccaagcgttt tttcatctct ttcatcccct catcaattct
     7021 tgaatgtcct gcattttctc tgacggtgca attcctaata accagcaaaa caatggagcc
     7081 gccagcagct gtggtacagt ttgttccttc agcttttggc gtttatttga aatcaaataa
     7141 aaaggaactt cacgatgtgc agccaagctg ccgccatgca gtccgtgcgc atccatcccg
     7201 tgatccgcag tcaccacgac ttgataacct ttttgcagcc attcaggaag gtaaatagcc
     7261 aataatctgt ccgcctgatt aaccgctgct gcatattcat gagaatcggc ggtaaatttg
     7321 tgaccgacat catcaatatt catcgaatgt acgaccaaaa agtctggctg atactgattc
     7381 actaaataat gggcatccgc aaataaatgc gaatcagggt aagtgtcctc actataaaag
     7441 atgccatgct gaatcagtcg ctcctcctga tgctggatgc gatcaaccaa acgattatag
     7501 ggggcttgat tgtacaactc gctgtaccag tggtaagcgg cggctcctgt ttttccgcca
     7561 gcttccttaa ctaattcaaa cagcgaccac tctttggatc gctgagcatg gtaattacta
     7621 tagatgccat tttcataggc tggaacgccg gtcatcagta cttcgtataa cggacgtgaa
     7681 ttgctgggca gctcactttt taccttcaat aagcttgctt gctggtgctc aactaaatga
     7741 ttcatgaacc caagctgggc gacggctgtg tcgtagcgac agccgtctag tataactaca
     7801 atcagtttgt caagtctact ttgcataggt cagtacctcc gcctgccaaa gctctggaag
     7861 ctctacagag gtcttgttcc aagcttcatt gtcttcaact ggcaccgccg cttcataagc
     7921 atcctccggc agtaattttt cagctacttc tttaggcagc tctaccttgc gaataggtcg
     7981 cgcatagccc tttgccaaat tgatttgccc ttcatcagat aaaatatatt cccgtgctag
     8041 ctttgccgca ttgggatgag gggcattttt attgattaag gttgtatacc cgctaatgat
     8101 ggagccgtct gttggcacag ttacctcaaa acgctcggag tcaatttggt cacgataatt
     8161 tagtccattg aagtcccaaa caactgccac atcgatttca cctttttcaa gatttgctac
     8221 gctggcatca acggtggaca aacggccttt ttctgccatt tttttgtagt aattcagacc
     8281 tggctgaata tccttttcgt cacctccgtt ggcaatcgcc gcagccaata cagcaaattg
     8341 tgcttggttg gcaatcgtca catcccccac gctaacttga tagtcaccct cttgcagctc
     8401 cttccagcta gtcggtgctt gcttcacatt ctctttatcg gtgataaagg aaatggttcc
     8461 cgtatagctt aacaaccaat ccccatcatc atcctttgcc caatccggaa tttcctccca
     8521 atagttggtc ttataaggta aggttaactc tttgctttca gccaacggac cgaagctgat
     8581 accgacatca ccaatatcgg cggttccatt ttttccttct gattcaaact tagccaattc
     8641 ttctgcactg gacatatccg tatccacgtg acttaagccg tattcttttt caaggtcgtt
     8701 ccaagtgcct acccaatttg cccaagtatc cggcattccc aagctggcaa tctctccttc
     8761 ttccttggct ttttctataa tcgtatctaa agagtcgtct gccgcagccg attgcttcgt
     8821 tgcttcacct gagccgcagc ctgtcagcag gatacttact gttgctgcac aaaataaacc
     8881 gatagtcatg ttacgaattt tttcattcat tttcattcat tcttctcccc tttcgataat
     8941 tccattctaa caaagctttg taatgagaga atcagtttct tgaaaaggtt tcatgaacaa
     9001 tgtaaaggtt gtatatggtt tgtaaatttc gacgaattcc cgttaacctt ctaaagcttt
     9061 tgcggtatga tagtagaaaa aaggaggctc cctatgcagt attatgatca gcatctccac
     9121 acgtattttt cctttgattc agaggaaaaa tttgaaaact atttagccta tcagccggag
     9181 tttttcgttt ctacggatca ctttgattta aacaatccat ctaccggaaa agatgacatt
     9241 ccggattatc cagcctatat aaacaaactg gactctttaa aagaaaccta ttccaccaaa
     9301 tttctgcgag gtatcgagat tggcgttgtt ccaggacaag aagagcggat cgtggattac
     9361 ctcgctcagc atccttatga tttaaagctg gtaagtattc atcaaaatgg ccgctttgat
     9421 tatatggacg atgtggtttt aaccaaagat cccattcaaa ctaccaagga atattttgat
     9481 cagatgttgc aggtcttgac ggattttcca gaaggagata ttttgactca ctttgattat
     9541 ggattacgcc gcttcacctt cacagcggaa gaattcgcgg agcattttga ggctcagctg
     9601 ctaacgattt tcaaaaaggt tgttgaacga gaattggcga tggagttaaa tgccaagagc
     9661 ttcttgaaat acggcaacga agacctttac cgttatgccg ttcctttgta tcaaagtgtc
     9721 ggcggaaagc tctttacctt aggctcggat gctcatgttg ccgaagatta tcagctgggc
     9781 tttaaaaaaa tgagtcagct gttggcagac tgtcaggtag aaaagttgct agtcattcaa
     9841 ggaaaagaac gccgcctgac gccgctgccg attttgtgat tcctatataa tagaagaaag
     9901 tggtgaaaaa aatgagcaac ctgtgggact acaatcaaga ggcacccatc cattatttaa
     9961 ttgcgcgaca ttgggatgcg ttgaaaattg aggcggtttg ccgatccttg ttagcagccg
    10021 ttccaaaaca acaactggaa aattttttgg tggctgacag cttgcagcgg gaaaaagttc
    10081 aggcttattt tgcagccttt aaagatcagc ctttggaata tttgcatgcg cagtttcatc
    10141 ttttttatca agtagccgcc ccagatgact acaacgattt acgaggacag ttgcaattga
    10201 cctttcaagc ggatgaaacc gcctataccg ttttattggg aatggctcgc cttggtgatc
    10261 aggcgaaggt cgagtggcgg attttcgata tttgatggca ggatcaaggc gtttcagttg
    10321 caaaccttcc tctctttcaa ttagaaaaac cgcagcagga agaagtttgt gtgctggtca
    10381 ccgatgctgg tgaaattcgt ctccgcttat ttcctgacca agctccccta gctgtagcca
    10441 actggcgctc cctagctgag caaggcgttt atgacaacac acctttcggc tcgtgtcatt
    10501 aaggaatttg ttattcaagg tggcgctttg gatggttctg gtgatgaagc tgtttccagc
    10561 tatgagggct ttttcgctgg tgaagtgcat cgcggacttt atcattttaa tggcgccttg
    10621 gccttagggg accacggccc ccacaccaac ggcaaccagt ttttttattg tgcaaaacac
    10681 aaaggcgcag gctgacctgc tgccgaggct cttgcttcct cagaatgtaa agcaagcata
    10741 cttggagctt ggaggactac cggagctgga tggtcgttat actgtttttg gacaggtata
    10801 ccaagggtta gaaatcgtag cagcgatcgc agctcagccg acaaacagtg aagatgtacc
    10861 gattgagcca gtgttcattc gacaaattaa cttcgaaaaa acatcgtaaa aagacactca
    10921 ctgacttcag caaaagctct attcttgagc tggctgcagc tagtgagtgt ctttcattta
    10981 tccataagat aaagcttgga ttcaataaat aaataaatgt tttggttaaa gctgtgacca
    11041 gcaaatgagg aaccgtgcga gccagttgat ctgccaatgc aaacatttct ttaggtgaag
    11101 gcgctggcgt gtctgccaac ggatactcca gtcccaatgc ttggtaaccg tgcataccat
    11161 agcagtggta cggtagtaat tctaacggta cgaaccgatc aagatctgcc acaaaacaag
    11221 caatcgtcag cagctcggtc aggttcatat tgatacctgg aaccacagga actcgaattc
    11281 gcacttcgca gttaggatac tcaattaaaa agcgacgaat attcttcaaa atttgttggt
    11341 tatctaagcc ggtccattgt ttgtggagct tgctggtaaa catttttaca tccacaaaca
    11401 atgtatccac atagggagct accttttgga tttcattata agcaccacaa aagctggttt
    11461 ccagtacggt attgattcca atgtatttcg atttttgcaa aatttcttta gcaaaatccg
    11521 cctgaaccag tgcctcgcct ccgctaatgg tcactccgcc atccgagtga aagtaaaaaa
    11581 cagcatcctt ctcaatctca tccattacct cttcagcggt catggtctgg ccaaatcctt
    11641 tttcaatctg cttgctttgt gattcgggaa tcgaacacca ggcacagcgt aatgggcagc
    11701 cctttaaata aacaacggtc cgtaaaccag caccatcatg tagagaaccc tgttctattc
    11761 ttaatactgt tccttccata tagatttttg aattacacac tagtttaaac taaaacacac
    11821 aaggaaaaac ttaccattta atcataaaat tagctcatat aaatatacta ggatgtaaaa
    11881 tagactggtc aaaatctggt caaaaaagag gactaataca ggtagtcctc ttttttgtta
    11941 ttttaatatt ctaaatcatt ttttattggt ttattccctt attcgatgta gactcaaatc
    12001 actatatcac tagatatcaa acggcgtcat acggaatcaa aaacatcaaa tttgacctgt
    12061 taaatggtca ttgaataaca ttcaaaatca ataaaacgga gtcagacacg gagtcaaaaa
    12121 cgctgagagt aaaaaatata tacagccttg taggtaaagt accacagggc tgtatatttt
    12181 ttatttcaga ttaccttaaa tgattccatt tactttttat ctcatttttt cctactagtt
    12241 agtatcaaat actctcctat tttctcaata aactattttt ggatacatgg tatctgaaac
    12301 agctaaaacc ttgtcattac agcaaaaaat cacgttatcc agactgtaga caaagtccat
    12361 tttatggatg atgtctacag tcttttcttt tataatagat ttatgaaaac gaatgcaagg
    12421 agtgatagct atgatgtcta aaaacaatac gaatggtaga aatcaatttg cgatgcttac
    12481 gattgatgat ctagttcctc aagatcatct cgttcgaaag attgatgccg cgcttgattt
    12541 tgaatttatt tatccaatcg tcgaagccac ttactccgat ttaggtcgcc caagtatcga
    12601 tccagtgatt ctgatcaaat tggtgtttat tcagtattta ttcggtatcc gttccatgcg
    12661 acaaaccatt aaggaagtcg ataccaatgt cgcttaccgc tggtttttag gctact
//
LOCUS       DAXWOO010000078        12456 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.078, whole genome shotgun sequence.
ACCESSION   DAXWOO010000078 DAXWOO010000000
VERSION     DAXWOO010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.078"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..154
                     /locus_tag="ACGE70_001682"
     CDS             <1..154
                     /locus_tag="ACGE70_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFF8693318.1"
                     /translation="SLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTL
                     RYSLGY"
     gene            complement(208..486)
                     /locus_tag="ACGE70_001683"
     CDS             complement(208..486)
                     /locus_tag="ACGE70_001683"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693319.1"
                     /translation="MENKVGYVRYNFFSSTPIMKDFASFNEALAVELSHDRERIHYEK
                     HVIINDLWNTEKTDLLALPDTDYPVFKEIEVKANKYNEIKQNKITNLS"
     gene            complement(601..1209)
                     /locus_tag="ACGE70_001684"
     CDS             complement(601..1209)
                     /locus_tag="ACGE70_001684"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693320.1"
                     /translation="MTDINTIRNLRNNHDKSINHIAKELDVNWRTAKKYADDDLLPQP
                     KARKKTGMMYTEKWGGIVSLWLSEDAKLPNKKRRTAEAMTTALKADGFPGSYRTVCVF
                     IQDWKATHYLETLEDQGFERLEHPPAEAQLDFGTMEVCHDGAFKDVKVLVMTFPFSNA
                     GFAVVLPAENQECLLEGMKELFSQSGGVPRKIRIDNMSTAVT"
     gene            complement(1613..2437)
                     /locus_tag="ACGE70_001685"
     CDS             complement(1613..2437)
                     /locus_tag="ACGE70_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFF8693321.1"
                     /translation="MEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAGANKKLV
                     YNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLKILTTFL
                     YVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKFVYDDFS
                     DELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGIPYINNL
                     GLPEQEVMKIGNKLNDSRVEKILTCHCTGSKAFNILKTQLGNKLEAIKTGQHLEIS"
     gene            2780..3196
                     /locus_tag="ACGE70_001686"
     CDS             2780..3196
                     /locus_tag="ACGE70_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFF8693322.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            3193..3663
                     /locus_tag="ACGE70_001687"
     CDS             3193..3663
                     /locus_tag="ACGE70_001687"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFF8693323.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            3677..4450
                     /locus_tag="ACGE70_001688"
     CDS             3677..4450
                     /locus_tag="ACGE70_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFF8693324.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAASPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            4464..5276
                     /locus_tag="ACGE70_001689"
     CDS             4464..5276
                     /locus_tag="ACGE70_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFF8693325.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            5330..6295
                     /locus_tag="ACGE70_001690"
     CDS             5330..6295
                     /locus_tag="ACGE70_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFF8693326.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            6432..9155
                     /locus_tag="ACGE70_001691"
     CDS             6432..9155
                     /locus_tag="ACGE70_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFF8693327.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESQILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGGSIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            9168..9800
                     /locus_tag="ACGE70_001692"
     CDS             9168..9800
                     /locus_tag="ACGE70_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFF8693328.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            10008..10232
                     /locus_tag="ACGE70_001693"
     CDS             10008..10232
                     /locus_tag="ACGE70_001693"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFF8693329.1"
                     /translation="MQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEVI
                     PENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            10334..10567
                     /gene="relB"
                     /locus_tag="ACGE70_001694"
     CDS             10334..10567
                     /gene="relB"
                     /locus_tag="ACGE70_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFF8693330.1"
                     /translation="MSQSTITFRIPDDEKELVLEYAKAHNSSLTELYRNAVLDKIEDE
                     IDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            10557..10826
                     /locus_tag="ACGE70_001695"
     CDS             10557..10826
                     /locus_tag="ACGE70_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFF8693331.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
     gene            complement(11005..12306)
                     /locus_tag="ACGE70_001696"
     CDS             complement(11005..12306)
                     /locus_tag="ACGE70_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFF8693332.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT         4064 a         2015 c         2444 g         3933 t
ORIGIN      
        1 ttccttagaa aaattaggtt gtcaaaattt caactcacgt tctgttaaag ggctagagag
       61 tagatttgaa agcatattac tggcttacag tgttctatta agtcgagcac aacgacgttt
      121 tgaaggaact ttgagatatt ctttaggata ttaaaattaa ctagcaccac gggtaaaata
      181 aatagttttt ctatcaagta tattgaacta cgataaattt gttattttgt tttgtttaat
      241 ttcgttgtac ttattggctt tgacttcgat ttctttaaat actgggtaat cggtgtccgg
      301 caaagctagc aaatctgttt tttctgtgtt ccatagatca ttgataatga catgtttttc
      361 gtaatggatt cgctctcgat cgtgtgacag ctccaccgct aatgcctcgt tgaaactggc
      421 gaaatccttc ataatcggtg tagatgaaaa gaagttgtac cgtacatagc cgactttgtt
      481 ttccatacta cctttctcgt ttccgctacg tggattgcac acctgtgtct caaaaccata
      541 gtgcatagaa aatcggagga aaccgtcagt cagcaccgct tgttcagtct ttgtttttgt
      601 ctaagtaacg gccgttgaca tattatcaat ccgtattttc cgaggaacgc cacctgattg
      661 gctgaagagt tctttcatgc cttccaacaa acattcttga ttctcggcgg ggagtacaac
      721 ggcgaatcct gcattactaa aagggaaagt catcactaat accttcacat ctttaaacgc
      781 tccatcgtga caaacttcca tcgtaccaaa atcaagttgc gcttctgctg gcgggtgttc
      841 aagacgctca aagccttggt cttccagtgt ttccaagtag tgtgtcgctt tccaatcttg
      901 aatgaataca cagactgttc ggtaggatcc tggaaagcca tcggctttta aagcagtagt
      961 cattgcttca gcagtcctac gctttttatt tggcagctta gcatcttctg aaagccatag
     1021 ggaaacgatg cctccccact tttctgtata catcatacca gtctttttgc gtgctttagg
     1081 ttgcggcagc aaatcatcat ctgcatattt tttagccgtg cgccaattta catctagctc
     1141 ctttgcaata tgattaatgg atttatcatg attatttcgt aaatttctga tagtattaat
     1201 atcggtcatt gtgagcatac ctttcttacc tccagttaca gtgtcgagac tataacttta
     1261 gaggagtttc aggttgcccg caatggcttt ttatcttaac ttaatcgatt tgcggagttg
     1321 tgtagttttt cgttgcactt gtatgtactt ttattctgcc ataaacagct taatgaatgt
     1381 ccacttgaaa aagtgaataa acgaaataga tgactcgatt taataaggaa gtaaacccac
     1441 acagaaatat aggtgatttt ccttgaatga ccaagatatt gaaagttagt gaaaaaacac
     1501 aaaaaggtag ccctattcac agaaggtctg ccttttgtgt tatcgccagc aaacattaga
     1561 gggggtattt catatagctt ttgtcctctc gacagtccta tcagtttctc ttttaactta
     1621 tctctaagtg ctgtccagtt ttaatagctt ctagcttgtt tcctaactgt gtttttaata
     1681 tgttgaaggc ttttgaacct gtgcagtggc aagtaaggat tttttctact cgtgagtcat
     1741 tcaatttatt tccaattttc attacctctt gctcaggtag acctaaatta tttatataag
     1801 gtatgccaat catatggaag ccaccaatta ggtattttat gggtttagtc ggaaatgttt
     1861 caagagcaat ttccatcata tcgataatgc catgatgact acagccagta aagacaatca
     1921 gaccgtcttc ttgctcaatt acgaccaata attcatctga aaaatcatca taaacaaatt
     1981 tatcatccat ctttttgtat aaattcttgg tggtcgagtt tgctttgcct agggggatga
     2041 tatggaagct cggagcaagt tctgttttcg tttttattgt atgtaaattc tcatgcattt
     2101 taaggatttc atttttagga ataccaacat acagaaacgt ggttaaaatc tttaaataat
     2161 ggggtaaagt tacaccttca gctgtataaa taggggcgtt tcctgatttt aaatatctta
     2221 acccgccggt gtggtcagaa tgaccgtgtg aaataatcac tgcctccaga tcattcaaag
     2281 tttcccccat tttagtcgca ttatagacta attttttatt agcgccagca tcgaagagat
     2341 atttcttccc tgagtagtta attaagagag ataacccatg tccactttta aagttcaaat
     2401 ttattcttga gttttcaaat aaaaccttga tttccactat aacatctcct ttcacatgaa
     2461 cgtgtattca tatattgtag cacattaagt tctatttttt gttgtaatta ccgaaaaaaa
     2521 tattaatgga cgctttttac gctttatttc gtatgtggaa gaaaatttct taggatgtgg
     2581 cgggggattg gggcaaagct aaattccaat tacagtgatc atttaattag acacctttaa
     2641 atacccacac tttcatcaac actataattt agggaaagaa aagtgttggc atgatataga
     2701 aaaaccttta attaacagcg tttcctaaaa ttggcacgca tcttgcttat agaaaagcgt
     2761 aaggatagga ggaatgaaaa tggcaactat aattcttgcc agccatgggg aatttgctca
     2821 aggattaaag caaacagcga caatgattat tggagatagt gtaccaattc atgccttgtc
     2881 agcatttaga gatgaagatg aatctatttt ggtacaaata aaaaaacttt tagccacaat
     2941 agatatagaa gaaacctaca ttttaactga tattctaggt ggtagtgtga ataatgatat
     3001 gctcactatt attaaagagt atgagggatt gcaattaatt actggaatga acttaccttt
     3061 agttatttct attgcaacct ataatggaaa aattggggaa agtgagctag aaaagcttat
     3121 taaagagagt caacaatcag tgattgattg tagaaaatta ttagtagaac aacttactgg
     3181 aggagatgac ctatgattaa gttagtacgt attgaccatc gtttgttgca cggacaggta
     3241 gtattttctt ggagtaaatc attaaatatc aatcgtatta ttattgccaa taaagaggca
     3301 gcgaatgatg aattcaaaaa aatgtctttg aatttatcta aaccgactgg cattaaatta
     3361 acaatttttt cagttgaaca gttactagaa aaaatgccta aaatcgaaac acttaacgaa
     3421 aatatcatgc ttatttttgg taatacaaaa gaaattcgtc aattttgtga agcctatcct
     3481 aaagtacaag aaatcaacta tggagggata atcaaaaaag aaggttccaa gcaatttagt
     3541 aatgccattt tcttgaatga atctgagcaa gaagatgcca aaaaattaaa agctcttggc
     3601 attaaacaat ttattcaaca ggtaccaact tctaaaaaag aagatttgaa tacaatgtta
     3661 taaaggagga atgaaaatgc tcgttcagtc aattggttta gggttaatcg gtgttttttg
     3721 tattttagac tcaaggcttt taggaagaat gaattttgaa cgaccgttga ttgtcagtac
     3781 gcttgttggt ttggtacttg gagacatgga aaaaggatta attgttggag cctctttgga
     3841 attgatgtcc ttaggattag taaatatcgg agctgcttca ccacctgata tgaatatggc
     3901 atcaattatt gctacagcct ttgccatttt atcaaatgcg aatgcagaag cagcattaac
     3961 gattgccatt ccaatttcag tgttaggtca aatactaggt atcttaatgc ggacgatttt
     4021 gtcaaatttg actcacgttg cggatcgctt tatcgaacaa ggaaaattta gccaagcaag
     4081 gagaattcat atactttatg gctcgggatt atatgctctc atgtatttta ttcctatttt
     4141 tctagcaatt tattttggaa cgaatcttgt ttctcagatt gttgatgcga ttcctgagtg
     4201 gctaacaaat ggcttaacgg ttgcaagtaa aattttacca gcatatgggt ttgcgttgtt
     4261 attacaaacg atgttaacga aaaaaatggt gccattttta ttacttggat ttttaattac
     4321 tgcttattct ggattgggaa tcattggtat tgcagccttc gcatgtattg ttgcgtttgt
     4381 catggttcaa gttcaaggta aaaactcaaa tggtaaccaa aatgagccag atccattaga
     4441 tgacttataa ggaggtagat taagtgaaag agaaagatca aaatgcatgg aaatatcggc
     4501 agttcttatg gcgttcatgg gcaattcaag cctcatggaa ctatgaacga caaatgaaca
     4561 tgggttttat gtacggaatt gcaccaattc tagataacat ttacaagaat ccagaagatg
     4621 aagcaatgaa gaaagaagct tacgagcggc atatgatgta ctacaactgt acccctcaaa
     4681 caagtgcatt tgtgttaggg ttaactgctt ctatggagga gcaatactac gaagaccaag
     4741 aaaactttaa tccggaatca atcagtgcag tgaagacttc tctgatggga ccgctatctg
     4801 gtgttggtga ttctttcttt caaggaacga ttcgggtatt agcctttgga ctaggaatta
     4861 atttagccca acaaggtagt attttaggtc caattctagc aatgttaatt tcctttgtac
     4921 cttccttttt agttacttac tatggtggga aaattggcta taacatgggg aataaatact
     4981 tgtctaaact gtatcaagaa ggtttgatgg ataaagtaat gtatgtttgc tccattgtgg
     5041 ggttgatggt cattggttcg atgattgcca gcatgatcgg gattacaaca ccaattcaaa
     5101 tgggtaaagc ttttgtctta caagatgtat tagatagtat tatgccacaa tttttaccat
     5161 taggattaac cttcttcatg tactggttat tgcagaaaaa ggtcaaaaca ggatggatgt
     5221 tggcaatctg tttagtcgga ggaattattt ttagtttgtt aggtatctta caataaggga
     5281 ataaccaata aatttggttg aataatataa ataaggagga attcaattga tgaattcaaa
     5341 agaaattgtt aaagaaatcg tagcgaaaaa agaggtaaaa tcaatcgtat ttgttggctg
     5401 tggcgcttca aaagcggacc tttatcctgc aaagtatttt ctagaccaaa atgcgaagca
     5461 gttacggatt agtcattaca cagccagcga atttaactat gcaacaccag catctgtaga
     5521 tgagtcaact gtcgttattt cagcttcttt aggaggtacg actccagaaa cagttgaagc
     5581 taacgcagta gctaaagaaa aaggtgctac agttattagt ctaacacgaa gcattgactc
     5641 accgttaaca aaagatgcag attatgtaat ttatcatcga tttgcagaga actatgctgc
     5701 taaattagaa aaaacaggct actgtttaga attggcagta gaacttttac atcaagtaga
     5761 aggttatgac cattatgaag caatgcaaga aggatttgct actatctatg atcttgccca
     5821 aaaaagtgca gaatcagctc gtaaagatgc gaagaaattt gctgaacgct ttaaagatga
     5881 aaaagttatt tatgtaatga gtagtggtgc tacacatgaa gtagcatact caacatcaat
     5941 ttgcttgatg atggaaatgc aatggattaa ttctggtacc ttccattcag gagaattttt
     6001 ccacggacca ttcgaaattg tggataaaga cgtaccgttt atattattaa tgaatgatgg
     6061 aaaaactaga aatatagatg ctcgagcatt aactttcctt gaacgctttg atacgaaaac
     6121 agaagttgta gatgccttgg attatggctt gtcttcacat attgctaaag aagtcttaga
     6181 ctactttaac ccattcgtga ttactgcagt atttagagtt tatgcagaag aattgtcaga
     6241 agcaagagaa catccattaa ctaagagacg ttatatgtgg aaactaactt attaaaaaat
     6301 agatcaaact aaataaggtg gcttgagaaa ttaagccacc ttgttttctt caatgtgatt
     6361 ttatacatat ggtaaaataa ctagtacata tggtaaaata actagtacat attgtgaaag
     6421 caggtggcaa gatgcagcga ttaaatgact atctttatca actaacattg cttttgaaat
     6481 ctacgccttc agataattat tcagctcgca gtatttcgca agctctacag ttgaatagaa
     6541 gtacaatcag tagctactta aatgaaggtg ttcgtgaagg gcttttcata aaagtaaaaa
     6601 gttacccggt tttattttta catagaacag ctcttgaaga actacatatc acactgaata
     6661 actccgaaat agaatcaatt gaaagcttgt taacaatttc tcaaaaaccc gcattagatc
     6721 aagtaattgg ctctaaagga agtttgaaag aagcaattga tcaaattaaa acggcagtct
     6781 tatatcctgg aaaagggttg cctttattat tgatcggcgc tagtggatca gggaaaacct
     6841 ttctagcgaa taaaatatat gaatatgcag tagaagaaaa ggtcattcac acatcggcac
     6901 ctttcataga ttataattgt gcacaatatg tgagcaatcc agagttacta tcctctgctt
     6961 tatttggcta cacgaaagga gcatttaccg gagcaagcca ggagcatact ggtttgctag
     7021 aaaaagccga tggaggagta ttgtttttag atgaagtgca tcgcttatct gaagaagggc
     7081 aagagaaatt atttacattt atggatacgg gtgaattctc tcctatgggg gataatagta
     7141 ttcggaaaaa agccgatgtt cgattagtct ttgcaacaac agaaaatata tatacgactt
     7201 tcttaccaac ttttcttagg agattgccag ttattgttaa tctacctaga tttcaacaac
     7261 gtccgtcgtt tgaacgtctg tcattgattg atgaattttt tgtttctgag agccaaattt
     7321 tagctaagga actatctgtt tctgatgcat tgattcattt cttaatgaat agtgattttg
     7381 aaggtaacgt ggggaatatt aaaaatatta tcaagtatgc ttgcggaaac gcatatatcc
     7441 accagaagag tcagcaaaca attcaagtta aattattgga tatgccttta gagtacagtt
     7501 ataaattcaa ggagcaacta aataaaccca gaaagaaaag agcagatcga atctactatc
     7561 cagaagaaac acgacaagtg caactagaaa gaaacaatca gctttttgtt caattttttg
     7621 accaattact tactggattc tctgaagtga ttgaaaataa caaggatatt aaactatttt
     7681 tagaagaaat ggttaccaag gtaacacaag taatggatac gcttattttt caagaggatt
     7741 acgaaaaaga gcagtcttta tacgccattc taaactatca tattcgtcaa agtattgatt
     7801 ttatgcgaga aagctacggg tttgaacaag atggtaaccg ggtagtcgct ttggccaact
     7861 atctatatgt caaggataat caagtgattt ttgaaaatag tcttcaagaa aagaaaacca
     7921 aagatcagct taacgaattt tttgctaatg ttcttgaaac cccttattgg tatgcaaaaa
     7981 aaatcttaag ccttttatct gagtgtttag atcaacctgt gtatgaagag gacattttct
     8041 ttattacgct atatttttat agtttgaatg tcacgaatgt taaaacaggt attaagagta
     8101 ttgtgctagc tcatggttat tctacagcta gtagtatggc gaatgttgcc aaccgtatgc
     8161 tgaataagaa cgtttttcaa gcttatgata tgcctttaga tattacatta gagaaaattg
     8221 aagcaaaatt gattcaattc cttggagatt atcgaacaga ttcaggcttg attttattgg
     8281 tagatatggg ttcactaaac caactaggcg gtttattagt gaatcatcta aatggaccac
     8341 tgttaatcat tgatcatatc aatacaccgt tattattgga agtcggccaa caagtattaa
     8401 acaataaaag tatcaccgaa ataggagcag aaattgaaac aagtcttact ttcaaaaagc
     8461 agttactact tcccaagaaa aagaaaaaga aagccatcat tacttgttgt tatacaggga
     8521 ttggtagtgc gacccagatt caggagattt taacgaaatg cttgaaagat gcagttagag
     8581 aattagtaat tattccgtat gattacaaaa agctgttaga aaacaagaat tttgagactc
     8641 cttttcaatt atacgatgtc atcatgattg taggtacaga agatcccaaa attaatcaga
     8701 ttccatatat tggattagat cgcctgatta atgggggaag cattggcgaa tttgttcatt
     8761 acttggaaaa agagctgact attgaagtga acgatttaca aaaagaatta attttcaatt
     8821 tttcaattag taaaattgtc gaaaatttaa ccattttgga tccagataag gtgctatctt
     8881 ttgtacaaaa agcaattaaa gaattatcga agttattaaa ttttgatttt tctaataatc
     8941 agctgttttt gttgtacctt cactgtagtt gtatgattga acgaattgta cgtaaagaaa
     9001 gtgtggatga gcaagaggac attgaagaat atatgcaagc aaatggagaa ataatggata
     9061 aaattcatag cgcttttttt gagattgaaa gggaatacac aattgaaata ccagatttag
     9121 agttgcgatt attgagcgat attgttcaag gataggagtg aattcgaatg acaatgggag
     9181 taatttttga tatggatggg gtactcatag atagcgagac attttatttt gagagaagaa
     9241 tgaacttctt tcaagaactg gagatagatc caggatcaaa ccagattgat gattatgtgg
     9301 ggaaaacaga agctgcaata tgggaaacat tagttcctga ggacaaatca ttaagagagg
     9361 aactttatcc gaaatatgta gattatcgaa aaacgcatcc aattgatttc gctaaagctt
     9421 tacgaaaaga agtgaaaccg ttacttgact tcctcaagaa gcgggatata ccattggcac
     9481 tggcatcttc ttctccagct gtcgagatag agagtatgct cattcaatgt gaacttactg
     9541 aatatttttc ctttgttata agtggtgagt cattaaaaca aagcaaacca catcccgaaa
     9601 tatatttaaa agcgaaacag caattagact gtgacttctt tattgcagta gaggattcaa
     9661 cgattggtat taaatcagca aaggcagcaa atatctatac ggcggcacta aaacaaccaa
     9721 tgcaaatcga tcagagtgaa gcagacatac aaattgaaag tctacaagaa ctggaacaaa
     9781 ttgttttaga aaaaggttag tgaattaatg gaaataaggc tatatatctg tgctagctat
     9841 tttgactaac caaaaaaact tgctataatt caggtgtagt aaaaggtgct ggtaacacct
     9901 tttacgaaaa tgcttcggca gtgataatgg gattggcttt ttaagttttt tattttgttc
     9961 gcaatcccta aggtcatgtg aatgatccta gggattgcta gaatattttg caacagaacc
    10021 aaaatacgaa tagttatcaa gcagccatat tgactgcggt caatttagga ggggatactg
    10081 atactactgc tgccgtaact ggtgctcttg cgggtattct ttacgggggt gaagtgatcc
    10141 ctgaaaattg gagaaaatcg cttcaaaatg accagcttct taaaacgatc agtcagagat
    10201 tttgcgatac cgtgctatct caaagaattt gaataatgga tatgatgcaa atcttgtttg
    10261 tactaacgtt gtattgacaa aaaggatttt atttagtaca atgttagtac aaatgatgaa
    10321 cggaggtaga atcatgtctc aatcaaccat tacatttagg attcccgacg atgaaaaaga
    10381 actggtatta gaatatgcaa aagcacacaa ttcttcattg accgaacttt atcgaaatgc
    10441 tgtactggat aaaatcgaag atgaaatcga tctacggact ttacggagtg caattgaagt
    10501 ttccaaagag aaaaaagaga gtggtatttc tcaagaggaa atggagaaac tgctaaatga
    10561 attataaagt catctacatg ccgcaagccc agaagcaatt aaaaaaaatg gacagaaacc
    10621 aagctaaaat aattgtcgcc tggatcagaa agaatttaga aaacacgact gacccgcgtc
    10681 agcacggaaa aggtttgact ggaaataaga gtggggaatg gcgctatcga attggaaact
    10741 atcgaatatt ggctaatatt tgtgattctg aaattgaaat ccagatattc tcaattggac
    10801 atagaagtac aatatataaa agataaccgt ttttggaacg aagtagagtt tcgaaagtca
    10861 atgtataatc ttttgaattt tgcataatac cacaggtaga aactacccga tactatcggt
    10921 tggtttctgc ttttttatgg ctctttgtca aataggactg atagagtgag ataagaacat
    10981 ctaaggtaga attccttttc tactttagat gttttttgat tcataggtaa tcagattatt
    11041 tagtaagaaa attctggtct tcattttccg aaagcttcga aagccataag cgactcgttt
    11101 caatgctttg atgtgggtat gcagattttc tattttggca tttgaataag gcaattcgag
    11161 ggctaaacga atgccggatt catagtttat aaaagctttt tttatagaat gcagttcttt
    11221 tggaacggaa ttgggcatcg ttcttacaag tttaaagaaa gtgtccgcct ctttgtcgtg
    11281 aaacgcttga agaatttctt gatagtaaaa ataggcgatt cgtaattcgt cagagatcga
    11341 aagcagacga tccaccactt cactttcggt gagatacgcc caaccaaaat tgcgccattt
    11401 ggtccatttg tcataaacaa gcttgtcctg tcgtttgatc agaagccgag acaacgcctt
    11461 taattgtcga taatatttgg cttgtgttgg atcgtttcga tcaaagcttt tcatgattcg
    11521 tacgcgaata tcctcaaatt tacgaatgag atgtttgaca atgtgaaaac gatccacaac
    11581 gagttttgcc tttggaaaac attcctttat cagagaggca taagaaacat tcatgtccgt
    11641 cacgatgacc tttaccttca atcgtgcgcg tcgttcaaat tgcataaaat aagaaacgag
    11701 agcctttttc ttccgtgaag gaagaatatc cagtaatttt ccagtatcgc catccataca
    11761 agaaaaggag tagggaccga cttgattttt cagtgaacga aattcatcca ttaataaaca
    11821 acgcggtaac caatttcttt ttacttttgt catggggatc agagaagcga gtgtccgaga
    11881 aacggtcgac caagaaacgc cgcaatgctt agcaattgtc gtcattgctt gtttttcagt
    11941 tagctcttct aaaattttat agcgcacttg tttagcgata aagcagtttc tatcgacaat
    12001 gtaggtattc gccgtaaaat aggtgtggca gccacgacag tggtagcgtt gtttcgctaa
    12061 agcaagataa gtcagttggt tcgcacattt attaagttga atgagcgatt ttttctttcc
    12121 gttcttaacg atcatgcctt catgattcat ctgtttacac aaaggacagc gacgaatttc
    12181 tttacttaac gtcgcagaaa caacaagact cttgatattc ttaatgctct tcgattccac
    12241 atttgtgatg aaaatgtttt tatctttgat tccaagtagt agtttgatat gatgaagatc
    12301 aggcatacag aacgctcctt tgattgtgtt gtggtgattc aattctaacg gagcttctgt
    12361 atgttttgtt ttttcatgct caaaagggac cgatagaatg attttctatc agtcctagaa
    12421 attatagagc cttttttatt tggtcttgaa gaagaa
//
LOCUS       DAXWOO010000079        12171 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.079, whole genome shotgun sequence.
ACCESSION   DAXWOO010000079 DAXWOO010000000
VERSION     DAXWOO010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12171
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.079"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(133..297)
                     /locus_tag="ACGE70_001697"
     CDS             complement(133..297)
                     /locus_tag="ACGE70_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693333.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            592..1920
                     /locus_tag="ACGE70_001698"
     CDS             592..1920
                     /locus_tag="ACGE70_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFF8693334.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2122..2955
                     /locus_tag="ACGE70_001699"
     CDS             2122..2955
                     /locus_tag="ACGE70_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFF8693335.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2971..3708
                     /locus_tag="ACGE70_001700"
     CDS             2971..3708
                     /locus_tag="ACGE70_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFF8693336.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3863..4831)
                     /locus_tag="ACGE70_001701"
     CDS             complement(3863..4831)
                     /locus_tag="ACGE70_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFF8693337.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5063..5896
                     /locus_tag="ACGE70_001702"
     CDS             5063..5896
                     /locus_tag="ACGE70_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFF8693338.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5930..6769
                     /locus_tag="ACGE70_001703"
     CDS             5930..6769
                     /locus_tag="ACGE70_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFF8693339.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6795..7355
                     /locus_tag="ACGE70_001704"
     CDS             6795..7355
                     /locus_tag="ACGE70_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFF8693340.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7554..9275
                     /locus_tag="ACGE70_001705"
     CDS             7554..9275
                     /locus_tag="ACGE70_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFF8693341.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9440..10582
                     /locus_tag="ACGE70_001706"
     CDS             9440..10582
                     /locus_tag="ACGE70_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFF8693342.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10598..11809
                     /gene="thiI"
                     /locus_tag="ACGE70_001707"
     CDS             10598..11809
                     /gene="thiI"
                     /locus_tag="ACGE70_001707"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFF8693343.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4148 a         2042 c         2527 g         3454 t
ORIGIN      
        1 aaagcttatg caaagaaaaa gaaatccaat cacttgggtt tctttttctt ttattattat
       61 gatgaatcaa gctttctgtt tttgtcacgt atgttgtaat ctttacgaaa gagtagagtt
      121 ggctactcat aatcaaccat acttcccatc atcgctaaat acgaaataaa tcagcattcc
      181 tacaccaatc aaagcacaag ctgcaccaag aaaagggatg acattcccaa agttatctgt
      241 attcataagt atgccaccac ccacaaacaa aaagccgcca gcaatcaggt ttttcatttt
      301 attcatctcc tttcttacgt tatagtattt ccttctttca atgagtattt tgaaagctat
      361 cttattgtat gatgttcttc taggcctctt ctttattata caggccattt tcgttatctc
      421 atagaaatat tcctttttat ctgaaaacta ttgtataccg tatgacattt ttcttcatga
      481 agttgtaagt atattctcat tcaaatgaaa atttcatcat agaagagatt gtatttctaa
      541 attcttaaga gtaaaatacg aagtataact tggatttgga aggatgattg gatgaaagct
      601 attacgcctg atatgacaac aaaatttaac aaacagtttc aagcaaatat caaacagcag
      661 gcattgcagc gggcagttgt gaagaacgga attactgctt ctgctgaaaa tgtttcagca
      721 catgtggaga atacaccggt attttctata gatttggcta cggggaaagt ggctaatcaa
      781 atgcaatcgg ggcgttgctg gatgtttgct gcactgaaca cattccgtca taagatgctg
      841 aatcaattcc atttaaaaga gtttgaatta tcacaaaatt acactttttt ctgggataaa
      901 tatgaaaagg caaattattt ctatgaaaac atcattgcaa caggaaaaga ggcaatcgat
      961 agccggaaag tagctttctt actcgctact cctcaacaag atggcggcca atgggatatg
     1021 atcgtttcat tattccaaaa atatggcgtt gtgcctaaat cggtgatgcc ggaaagtgcg
     1081 aatagttcaa attctcgaga tttgaataac tacttgaaca aattactgcg taaagatgca
     1141 gtagtgttac ggaaaatggt tgctcaagga gaaacattag acgctattga agaaaagaaa
     1201 gaagaaatgc tagaaagcat ttataacttc ttagctatct cattaggaac acctccaaaa
     1261 gaattcgatt ttgagtaccg cgatgaagaa aagaactatc atcttgaccg aggactgaca
     1321 ccacaaattt tttatgataa atacatcggt gtaaaattag atgattatgt tagcgtgatc
     1381 aatgcaccaa caaaagacaa accattcaat cgttcttata cagtagagat gctaggaaat
     1441 gtcgttggcg ggaaagaagt caaatatctg aatgttgata tggaaacttt caaaaaatta
     1501 gcgattgctc agcttgaaga aggagaatct gtttggtttg gttgtgatgt aggtcaatct
     1561 tctactagaa ctagtggcat catggcctta gatgcttatt caatggatga tctatttgat
     1621 acggatttta caatgactaa agctgaacgt ttagattttg gagaaagtct gatgacccat
     1681 gcgatggtat tgaccggtgt tgacttagta gacgggcaat caacaaaatg gaaagtcgaa
     1741 aatagctggg gcgaaaaagt tggaaagaat ggcttctttg ttatgagtga tgcctggatg
     1801 gacgaatata cgtaccaaat cgttgtaaga aaagatttat tgacgaaaga gcaattaaat
     1861 gcgttcaatg aagaaccaat tgttctatct ccgtgggatc caatgggtgc tttagcatag
     1921 acgagtattt caatgaagag tatgtagtaa agataaaaag agctggctta agccagctct
     1981 ttttatcttt aaagtccatt ccctgttttt tatggtccct gtgctataat tctaaataaa
     2041 ggataaggat tggaaataat ccttttatca ttaaaaatat gatgacgaag tagaataaaa
     2101 ttgggagtag gggggaaagt catggatatc caaactgttt tatctgatct gaaattaaat
     2161 ggtaaagtga ttccagttgt tggtggcgat gttaatcaga cctatcgtat aaaaaccgaa
     2221 catcgtgctt attttttaaa aatccatccg aatgtaaaaa aaggattttt tgaagcagaa
     2281 gtagacggat taaaagaact gtcagcattc gtacgtgtac cggatacgta tatgctaggt
     2341 gaaacaagtg aaggcgctta tttgttaatg gaatggatcg agccgggaaa aggggatcaa
     2401 cgagatcttg ctgcagcttt agctaatctc catcagcaaa cagcgccaca atttggtttt
     2461 cgtaaagata actatttggg tacacttgtt cagaaaaatt cttttgaaga agattggtgg
     2521 acttttttct ttaaagatcg gcttgaatcc cagatttctt tggcagaaga aacgaatcga
     2581 tggaatgtac agcgtcaaga aaagtatctg cgatttaaag aacgtgtatt gaaatcggtt
     2641 gaaccaaaaa agatcactcc acgcctcttg cacggagacc tgtggagtgg taatgtgttc
     2701 tttgaccagc aagggcatcc agtttttgtc gacccagctg tttcttatgg gaatcgggaa
     2761 caagatattg caatgagcca gttattcggt ggtttccgac cggaattttt agatgcttat
     2821 caaactatat ttccattaga aaagggatgg aaagaccgac tgccgattta ccagttatat
     2881 tatcttttgg cacatttgaa tatgtttgga gaaagttacg gttcccaagt agatcaatta
     2941 ctagaaaatt tttaagagga gggcgtgttt ttggcaaaaa aatataccaa aaagcatgaa
     3001 gtttcatatt atgagtgtga catcaaccaa acgatgacat ttccttcgat gttaggagta
     3061 gttatcaaaa cgtctgaaga tcaaagcgac cagttgggaa gaggcagtga attcgtcaat
     3121 agctttggac ttacttgggt catcacgaat tattccatgg agattaatcg tctccctaga
     3181 gtcggtgaag taatctcagt aacgactcaa gccatggagt acaataagtt tttctgttat
     3241 cggaacttct ggatccatga tgaagatggg aaagaattag tcaaaataga atctgttttc
     3301 gtattgatgg attttgtgaa tcgaaaaatg agcagtgtta atgaagagat cattgctcct
     3361 tttgagagtg agaagatcaa gaaaatcaag cgtcaggaaa aaattgaaaa gattgattcg
     3421 ggcttcatgt tgccatatcg tgttcgtttt tatgatatcg atagtaatca gcatgttaat
     3481 aatgcgatgt attttaattg gatcattgac gtattaggat atgacttttt gactacgcat
     3541 gttccagaaa aagtaattat tcgatttgat aaagaagtag agtacgggaa tgagatcgaa
     3601 agccattttg aacaaatccc tatcgaaaat ggtgtcaaaa caaaacatga aatccgaatg
     3661 caagaacagc tgtattgtga agcaaatatt atatggaaaa ataaataaaa aagaggttgt
     3721 gacagaaggg ggcaacttca agaaataaga agaaatttac gaaaattgtt tctcaaattt
     3781 ttgtaaattt tcgacttagt tttcgaagaa gctatttttg tcacagcctc tctttttaag
     3841 tttagctatt agataatgat gtttagtttt ggataaacga aatcaattct tcacccgaca
     3901 aaccatagtt tacagcaatc aatagtttta aacgtgcttt ttgactatta atactgttac
     3961 agaaaataat ccccattttt tctagttctt ttcccccgcc ttcataatca taaacaggct
     4021 ctgcgattcc attgaaacaa cgagaaacaa gtactacagg gattttttgg acaagaagct
     4081 tttccaaagc agctaaggct tgtggaggga gattccccgc acctaaagct tcgattacca
     4141 aaccgtctac tttcgtatgg gcgatggctt ccaataagtc cccttgcatc ccagcatatg
     4201 ctttaacgat cgggatagtc ccatctacag cggaaatgtc cagtcgcttt gtttctaaaa
     4261 gttcctgcaa gaataaaatt cgattcttag ttactaagcc gatgggaccc aatgtaggtg
     4321 ttcggaaagt agcaacattt gtggtatggg tctttgtcac gtagcgtgca gaatggatct
     4381 catcgttcat gaccaccaaa acccccttat ctagcgcttc ttcacaactt gcgacacgaa
     4441 tcgcactttc aaaattataa aggccgtcac ttccaagttc attacttgaa cgcattgccc
     4501 cagtcaaaac aattggtaat ttgttgccaa tcgttgtatc aagaaaaaaa gcagtttctt
     4561 ctaatgtatc tgtaccgtgt gtgatcacca ctccggatat acctgatgca taagcggaca
     4621 agatacgttc ttttaatacc aacatgtgag aaggcatgat gtgtggtgag ggaagattga
     4681 agatatcttc aactaataat tctacatgct tggggattgt gatttctgca tctaataatg
     4741 ggtttgccac atcaggacta acaccgcccg tgagcatgtt ttctttcatg gcgatcgttc
     4801 cgccggtgtg tagtacaagt atttttttca ttttacagac ttccttacga ataattttgc
     4861 atagccgtta aatcttattg tatactatat aaaagagttt tgctatcagt gaagaataac
     4921 gaatagccta acttatcata aaaaagctga ccttattaaa atggaaaaga ctcaaccttg
     4981 acctcctaat acaatggagt atctggttga gtttctttta caagaaagca gccaaggaaa
     5041 tcaatcacga aggtggccgg aaatgatcag aatcggatta acatcgttta aagaacatga
     5101 caaattaact ggtaaaaaga ataacactct ttatgaatat gcagggtttc tacccttagt
     5161 ggaattagat actgcctatt atggaattcc ccgaaagtcc tcagttgaga attggataag
     5221 tgaagtacct gaatcatttc gctttgtagt caaagtctat caaggaatca gcggtcaagg
     5281 agactggaag ccttattatc agaccgaaga agagatgatt gcgcattttt tagatgttat
     5341 gtcgccaatg gtagatgctg gaaagatgtt ttgcttttta gtccaattcc cctcatcatt
     5401 caaatgtacg aaagaaaatg tccagtactt gcgcaaactt agaaagtggt ttgagggtta
     5461 tcctgtttct atagaattgc gggattattc ttggtacagc gatgaatttc ttgaacgtac
     5521 aagaaacttt atgtctagag ccgctttttc tttagccatc atcgatgagc cacagttgct
     5581 gaatacaacg attccttttg atccatttgt caccaatccg aactttgctc tttttcgatt
     5641 ccatggtagg aatgcgcaag gatggcaagc aaatgataaa gagtggcgta aaaaaagaac
     5701 gttatatcgt tattcagaaa aagaactgca agaactagca catgaagtca aagtagttga
     5761 ttctaagaca aaagaggtag gtgtgatttt taataataat tctggtggtg atgcggctga
     5821 aaatgcatta gcactgcaaa aaatattaaa tatcaagcca gatggactca atccaaccca
     5881 aatggatctg ttttaagggc ttattttatt caagaaaggg gcgaagtcta tgatcaaagc
     5941 tatttttttt gatattgacg gaacattggt caccaatcgt tcaaaagcgt tggaatcaac
     6001 aaaacaagca attgaatatg ctcggaaaaa cggcatttta tgcggtgtag caacggggcg
     6061 cagcccagta aagatcaaag aaatcattga tgaattggaa ttagatatgt atgtcgtgta
     6121 taatggtcaa ctggttttta ctgcagatcg cacgattatc gaccaccctt ttgaacaaaa
     6181 agtattggaa catattgttg aatttgcaga tgagaaccat cgacaaatcg tttttggtgc
     6241 gagaaatcgg ttagatggat caacgacgat gctattagga caatcgattt ttatcaagcg
     6301 actagtcagc tttttaccgc gtaaattccc ggtacgtctg atgaaaaaaa tcctccaggt
     6361 ttttagccct caccgacaaa aggatcgtta tgaaacgtta gagatattaa aagaaccaat
     6421 ttatcaatgt atattgctca gtccggagtc agaacagaaa aagttagaac atcgctttcc
     6481 cgagtgtacg tttcagcgtt caaatacgta tactgtagat atcatcccaa aaggtggttc
     6541 aaaattacta ggcatccaag ctttcgcaaa tgctgtcgaa attgaaatgg aagaaattat
     6601 ggcattcggc gatcattaca atgatatcga gatgctgaaa ggtgtaggaa ttggagtggc
     6661 catggggaat gctcagatcg aagtaaaaca tgctgcagac tttgtgactc aatcaaatga
     6721 agaagatggg atatacgaag gactgaaaca cttcggtctg atttattagt caatgaaaga
     6781 aggaaaaaat cagtatggaa gcagagacac cttttcaaaa agtagaaaca ttccacaaaa
     6841 cttttgatcc aagaagacca gagataccca cggcttttac agaaaatgaa gctcatacac
     6901 gtgctgcgtt caaaatagaa gaacttgttg aatttgttgc agctacttca cctaataaag
     6961 aacaactgca tgaagctgtt atgctgctgc acgaagcatt agataaagca gaagaaaaaa
     7021 tacttggtaa aacacagtgg ggaagtgatc cgctggttga acaagtagat gcactgacag
     7081 atatccttta ctttacttat ggttcttttt cattactagg aatagtgcct gatcgaatat
     7141 tcgaaatcgt ccatcaggca aatatgggga aattatttcc agatggaaaa cctcattatg
     7201 accctgtaac aaataaagtg ttgaaacctg aaaactggca gatggaatat gctccagaac
     7261 ctaaaatcgc ggcagaaatc caacgtcaga ttcaagaagc gattgctaag aagaaatccg
     7321 ctgatatagg catgtgcaca aatgaaaaag agtgatcaga gtttcactat ttttgttatc
     7381 tttatacgat ggaaaaacaa aagattttcg tttaagttag tgaatgcatg aaaatagtat
     7441 gctataatct agaatagatg tcttttgcac gagagacgta ttatttggat ttatgccaag
     7501 cagaacgcca tctgttgcat aaaagatgca tactgaaaac ggaggaacca taaatgggaa
     7561 ataatgttat catcggaatc gtcattgcaa tcattatagt cgccattgtc gtctttttgg
     7621 ttagttactt gatgaagaaa aaaaaccaag atcgattgga tgccttagaa aaacgcaagg
     7681 aagaattatt tgacttgcca gtgattgaag aagtcgatga tgttaaaaaa atgcatctgg
     7741 ttgggcaaag ccaaaatacc tttcgtgaat ggagtcaaaa atggaatgac atatctactt
     7801 cctcgttcgc tgaactagaa agtcaaattt tcgaagtgga aaacctcaat gaaagttttc
     7861 ggttcttaaa aggaaaaaaa gcagttgttg aagcagaagc tacaatggac caaatggaga
     7921 aacaagtgac ggaaatccgt gaaggtctaa aggaattgcg ggaaacggaa gaacgcaact
     7981 cactagctgt tcagcaggca cttgacgtct atgaagaact gaaagaaaaa gtcagagaag
     8041 acgagcatga atacggacca gctctttttg aattgcaaaa acagatcaaa gtgatcgaaa
     8101 aagaatttac gcagtttgtg actttaaaca cttctggaga ccctgttgaa gcccgcgagg
     8161 ttttggataa agcagaaaaa catacgtatg aagtggaagc tctgatgaaa aaaattccac
     8221 cgctgtatga ggagctccat accactttcc ctgaacaact tgaagaaatc agtgatactt
     8281 acgagaaatt gatggaagaa caatatgtat tcccagaaga agatcttgct gaagatatcg
     8341 caaaagtaag tcgaagaatt gaaaattcat tagccaatct tgaaaaaaca gaagttgaaa
     8401 cggtcgaatt tgaaaaccgt gaaacagctg atttgattga ttcgttatat gatatcttag
     8461 aacgggaaat ggaagctcag agatatgtaa aaactaacca atcaacgatt gcagagtata
     8521 tcaaacatac aacaaagaat aaccgtcaat tattgattga gctggatcac acatctcaaa
     8581 gctatacatt gaaccacaat gaaattggcc gtgtacgagg tttccaaacc gaagtggaag
     8641 aaatggaacg tcaaaatgaa cagatgattc cacagatccg tcaacacgaa attccttatt
     8701 cagaagtgcg cacgttctat aaaacagtct tcaaagtctt agaagatatc gaaaagcaac
     8761 aagtagagat cgacgactca ttacatgaat tgagaaaagg cgaaaaagaa gcgcaagaaa
     8821 agatcgatac attcgaattc aaattgcgca gtttgaaacg ttttgtagaa aaacaacgtt
     8881 tgcctggctt gccgaatgat tacctagaat tcttttttgt tgctactgac cgtattgaag
     8941 aactaagtgt tgtcttgaat aaaattcgag taaacatgga agaggtcaat cgtttagttg
     9001 ctctatgtga agaagatcta gaattattag ataagaagac acatgattta gtagatgcag
     9061 cagcattaac ggagcaaatg ctccaatatg caaatcgtta tcgtcataca cacgaagaag
     9121 tgagagaagc aattgataaa agctactatt tattcaataa agaatatcac tatcaagaag
     9181 cattagacga aatcggtaca gcattagagc gagtagaacc tggtgctttc aaacgcatcg
     9241 aagattttta ttttaatcat ccagacttag tataaagcaa aaaagttagt aaaccagcaa
     9301 gagcaagacg gattattgtc ttgttcttgt ctttttttgt atatttgaaa attgaaacaa
     9361 gtgaaaattt cacctgctct tgaaagcacc ttggacaacc gttataatag aaaagaattt
     9421 caaaaaagag gggtttctta tgatatattt tgataacagt gcaacaaccc caatctttcc
     9481 gcaaagctta gatgcatatg taaaaaccag tcagcggatt attgggaatc catcaagctt
     9541 gcatgattta ggcaatcaag ctaaccggtt gttgcagcaa gcaagaaagc agatcgctga
     9601 tttgatccat gtagaaccag aagaaatcta ttttacttca ggcggaacag aaggagataa
     9661 ctgggtatta aaaggaacga tgatcgaaaa aagagaatat gggaatcata tgatcatttc
     9721 aggagtcgaa catccggccg tcagcgagac agcagaacaa ttaaaagaat taggaatcga
     9781 agtaagtatc gccccagtgg ataaacgcgg atttgtacgt gtcgacgaat tgaaagaatt
     9841 aataagaaaa gagacagtat tagtctctgt tatggctgtg aataatgaag taggatcgat
     9901 tcagcccatc caggaaatca gtgatctttt ggatgatttt cctaaaattc atttccatgt
     9961 agatgctgtt caggcgattg gaaaagtacc attctcgcaa tggttgacag aaagagttga
    10021 ttttgcaaca ttttcagctc ataaattcca tggcccacgg ggaactggtt tcatttattg
    10081 gaaaaagggt cgtcgtttag caccattact gacaggtgga gggcaagaaa gaaatcagcg
    10141 aagtgggaca gaaaatgttc caggtattgt ggctatggtt aaagctttac gtttatcatt
    10201 agagataaag gaacaaaaac ctgagcagac acaacgctta aaagtagctc taatcaatgc
    10261 gttgaagacg tatgagaaag tgacgatttt ttcagaagga actgactatg caccgcatat
    10321 tttatgtttt gctctaaaag gaattcgcgg cgaagttctc gtccatgcct tagaagaaaa
    10381 acaaatattt gtttctacta ctagtgcctg ctcctcacgt aaacatatgg caagcagtac
    10441 attgcacgca atgcatgtac caaatgatct ggctacatct gctgttcgaa tcagcttaga
    10501 tgaaaacaat tcgcttgcag aagtcgagca atttatgatt attttcaagc agctttataa
    10561 gaaattttct gcaatcaatt aaaagaggag tgttcttgtg aaatacagtg aaattatggt
    10621 ccgttatggg gaactttcga caaaaggaaa gaatcgaaaa agctttatca tgcaactagc
    10681 acaaaatgtc agacaagctt tgaaagactt tccagaaatc aaaatccatg ctgatcggga
    10741 ccggatgcat cttttattga atggcgcaga cgggcagcta gttattccaa aactagcaaa
    10801 aatatttggg atccaaaatt tctctccaag tattcgtgta gaaaaagatg tcgaagtttt
    10861 gaaacaagct gttcaagaca tcatgaaaga aatttatact gggaaagaaa catttaaaat
    10921 tatcgctaaa agaagcgatc accaatttga attgacaagt aatgaattga atcaaacatt
    10981 aggtaatgct gtttttgata ttttcccgca tatccaagtt caaatgcgtc aaccagatat
    11041 tccattacgt gtagagattc ggcgtgatgg cgcttatcta tcgtatgaaa cgatcaaggg
    11101 agctggagga ttgcccgtag gaacaagcgg aaaaggaatg cttatgcttt ctggggggat
    11161 tgattctcct gtagcagggt actttgccat gaagcgggga gtggaaattg aagctgttca
    11221 ttttgctagt ccgccgtata caagtgaaca agcactgcaa aaagcaaaag atctaacagc
    11281 caaactagca ccttatgtgg gaacgatcca ttttatcgaa gtgccattta ccgagattca
    11341 agaagaaatc aaaaagtcag tgccacaagg atattggatg acaattactc gtcgaatgat
    11401 gttgcgtttg accgatgaaa ttcgccgtat tcgtcatggt ttagtcattt tgaatggtga
    11461 atcattaggt caagtggctt cacaaacttt gcaaagtatg gtggctatca atgaagtaac
    11521 gaatacgcca atcattcggc ctgtagcgac aatggataaa cttgaaatca ttgaagtagc
    11581 agaacagatc gatacattta atctttctat ccagcctttt gaagattgct gtacgatttt
    11641 tgctcctcca cagccaaaaa cacgccctca actggaaaag gtccatcaat atgaagagcg
    11701 tttagcaata gatgaaatga tccaacgagc actagctggc ttgaagatgg aagagataca
    11761 agcagaatcg tctaatgaaa aaactgaaga attttcagaa ttgctataag aggttatgaa
    11821 aatagtgttt tgaccagagt attagagaaa caaaacgaaa aaatagctcc atgtatttta
    11881 ccgtttttga actaatgtcg aagattagct gattgaatac catttatcaa gaagttatgg
    11941 aaaagccggt tcttttttac taactgaaaa ataaaatttt atttagagat caagctacag
    12001 ttgataaatg atataaaaaa gagaatttcc catgaaatag cgaggttgtg acagaagtgg
    12061 gcagcttcaa gaaataagaa ggaatttacg aaaattgctt ttcaaatttt tgtgaaattt
    12121 cggcttattt ttcgaagaag ttacttctgg aacaccgtgt atcagaggtt g
//
LOCUS       DAXWOO010000080        12136 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.080, whole genome shotgun sequence.
ACCESSION   DAXWOO010000080 DAXWOO010000000
VERSION     DAXWOO010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.080"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..610
                     /locus_tag="ACGE70_001708"
     CDS             <1..610
                     /locus_tag="ACGE70_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFF8693344.1"
                     /translation="KRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWIQLIL
                     DEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSKITVF
                     PKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAYTIQN
                     ATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            723..1001
                     /locus_tag="ACGE70_001709"
     CDS             723..1001
                     /locus_tag="ACGE70_001709"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8693345.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            1119..1355
                     /gene="secG"
                     /locus_tag="ACGE70_001710"
     CDS             1119..1355
                     /gene="secG"
                     /locus_tag="ACGE70_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFF8693346.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            1458..2213
                     /locus_tag="ACGE70_001711"
     CDS             1458..2213
                     /locus_tag="ACGE70_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFF8693347.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            2216..4573
                     /gene="rnr"
                     /locus_tag="ACGE70_001712"
     CDS             2216..4573
                     /gene="rnr"
                     /locus_tag="ACGE70_001712"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFF8693348.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            4595..5059
                     /gene="smpB"
                     /locus_tag="ACGE70_001713"
     CDS             4595..5059
                     /gene="smpB"
                     /locus_tag="ACGE70_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFF8693349.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            5361..6080
                     /gene="atpB"
                     /locus_tag="ACGE70_001714"
     CDS             5361..6080
                     /gene="atpB"
                     /locus_tag="ACGE70_001714"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFF8693350.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            6133..6348
                     /gene="atpE"
                     /locus_tag="ACGE70_001715"
     CDS             6133..6348
                     /gene="atpE"
                     /locus_tag="ACGE70_001715"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFF8693351.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            6439..6963
                     /gene="atpF"
                     /locus_tag="ACGE70_001716"
     CDS             6439..6963
                     /gene="atpF"
                     /locus_tag="ACGE70_001716"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFF8693352.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            6950..7492
                     /locus_tag="ACGE70_001717"
     CDS             6950..7492
                     /locus_tag="ACGE70_001717"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFF8693353.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            7515..9071
                     /gene="atpA"
                     /locus_tag="ACGE70_001718"
     CDS             7515..9071
                     /gene="atpA"
                     /locus_tag="ACGE70_001718"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFF8693354.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            9085..9987
                     /locus_tag="ACGE70_001719"
     CDS             9085..9987
                     /locus_tag="ACGE70_001719"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFF8693355.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            10010..11416
                     /gene="atpD"
                     /locus_tag="ACGE70_001720"
     CDS             10010..11416
                     /gene="atpD"
                     /locus_tag="ACGE70_001720"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFF8693356.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            11430..11852
                     /locus_tag="ACGE70_001721"
     CDS             11430..11852
                     /locus_tag="ACGE70_001721"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFF8693357.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4035 a         2155 c         2677 g         3269 t
ORIGIN      
        1 taaaagatat gcagagaaag gattcaacgt tcttgtacct gatttacgtg cgcacggaga
       61 aagtgaagga gaaatcatcg gaatgggatg gctagatcgg ctagatttaa ttgcctggat
      121 ccaactcatc ttagatgaac agccgaatgc gtccattatt ttacacggcg gatcaatggg
      181 agcttctacg atcatgatgg caagtggtga aaaacttcca tcagcggtca aaggatttat
      241 tttagacagc ggatatgttt ctgtttatgc agaattccga tatatgttaa gtaaaatcac
      301 tgtctttcct aaaaaaatgg tcatgagata tgcgaatcat tatgcccaga aatacgccgg
      361 ttactcctta aagcaagcat cagctacgag acagcttggc agcaaccatc tgcctttatt
      421 agtcatccat ggtgaaagag atcattttgt ccctacagaa gcagcgtaca cgattcaaaa
      481 tgctacggca ggggataaag cactgctgct tgttccagaa gcagaacact tggaagcatc
      541 gatgaaagat ccgaaaacct attggacagt gattttttca tttattgaac aaagaataac
      601 gatggattaa ttttctcttc ttttttgtaa gtaattcgag gataaataat tgtttataga
      661 tctggagact gctaaaataa ggtaggaata aaaaaatatc agttatttga ggagtgcggc
      721 atatggaaat caaagaagaa aaaaatcgtc ttgctttatt caatgatgag caacaagaga
      781 ttggagaaat gacatggtca gatgcgggcc cagacatcat gatcattgac catacatttg
      841 tagatccagc atatcgtggt caaaagttag ctgaaaagct agtgtatact ggagtagaat
      901 tagcaagacg agacgggaaa aagatcattc ctttatgccc ttatgcaaaa aaagagtttg
      961 aaaagaaacc agaatatcaa gacgtcttac gtcagtcata agaataacag ctgaaagttt
     1021 tgaactttca gctgttattc catttatata gagttgtcac ggacttttat ctatgataaa
     1081 gttaaagaga cgatatcttt tgggaaggag gctgcgatat gtataattta attttgggaa
     1141 tcgtgatcgt tttatcgatt gttatggtca ttgcgattat gatgcagcca agcaagcaaa
     1201 atagtgcagc gagtgcattt accggcggtg cggatcaact attcggcaaa caaaaagctc
     1261 gcggctttga agctgtcatg cagcgatcaa ctgcagttat gggagctatc tggatgatct
     1321 tattatttgt cctagcatat ttatcttcga agtagttgaa taagcctctc gcaaaacggc
     1381 gggcggcttt ttttattgca aagagaacgc ttaagaagaa gttatgaaag aaactatggt
     1441 aaaatgaggc gaaagaggtg agacgagtga gacaattacc aaaacccctt tatgcaaaac
     1501 atggaaagcg agcggttttg ctgcttcacg cttactcagg aagtccaaac gatgttcgaa
     1561 tgctcgcacg atttttggaa aaagcagatt atacagttta tgcgccgctt tttaaaggac
     1621 acggaacaat ggagccatat gatatcttgc aagaaaaagc agaaagctgg tgggcagaca
     1681 ctaaaaaagc catccatttt ttacagtctg agcagttttc tgatattgcc gtattaggat
     1741 tatcaatggg cggcatcttt gctgtacgag cactagaaga agaatcagtg attggcggcg
     1801 gttttttctg ttcgcctttg tctcctgtaa aaacaaacgt gcctgaaaac tttgaaaaat
     1861 acgtacgtca agtgctgaag acagcaggta aatcagagaa agaaatcaat gaaaaagcag
     1921 ttgcttaccg ctcgttagct gaacaacaat taatggatat acaagatcag gcagcaatcg
     1981 tagaaagtag gttgtcagat atccaacagc cgatattttt agctcaagct gggaaagatg
     2041 aaatgatcga ccctaatggc gtatttgaaa ctgcacgcaa gttgagccgt cagcgagtga
     2101 cacttcaatg gtatcctgaa agtggtcatg tgattacagt tggtacagca agacgcgaat
     2161 tagaaaaaga cgtcttggaa tttttagaaa aattaccttg gaatgaggaa taaatatgac
     2221 aaaagaaacg ataaaagaga aaattctctt tttcatggaa agcagtcgca aacgcagttt
     2281 ttctatggaa gaaatcgctg aaggtttagg gttccaaaaa agcgatgatt ttaaattact
     2341 agtacaaacg attgctcaaa tggaaagaga acagagtata gttttcaata aaaaaggaaa
     2401 agtgaaactt cctttaaaac caatcttgat cgaaggaaca tttcgagcaa atgaacgagg
     2461 atttggattt gtaacgattg atcccgaaga agatgacatc tatatcccaa aagaagcgac
     2521 tggttttgca atggatggag acaccgtagc aatcgatatc atgaagcgag caaatacaga
     2581 tatggatcgc ggagcagagg ggaaagtagt cgagattcga gaccgggcga ctactcagtt
     2641 agcaggggaa ttcgttgctt attcagaaga agaaatacaa gaaacagatc tttacggcgt
     2701 tgttatcccg aaagatagaa agctcaacca atttaaagta tatgcagcag cagagggagt
     2761 tcgtccagtt gacggaagta ttgtgctgat cgaactgacc cattaccctg aaaaagggta
     2821 tgcgactagt ttagaaggga taatcaagca agtgattggt cataagaatg accctggcat
     2881 ggatatcctc tcaattgtcg tggctaatgg tatccctacg aaatttcctg atgatgtatt
     2941 gtctgaagca gatgaagttc cagatcaaat cagcgaaaag gatataactg gacgtagaga
     3001 tttaagaaat cagctcattg ttacgattga tggagaagat gcaaaagatc tagatgatgc
     3061 agttacggtt aaaaaattag ataacggtaa ttattttctt ggagtacaca ttgcagatgt
     3121 ttcttactat gtgacacaag gcagtcaatt ggacagagaa gcatatgaaa gagggacaag
     3181 tgtatatttg actgatcgag tgattccgat gatcccgcaa cgtttatcga atggtatctg
     3241 ttcactgaat ccccatgttc caaggctgac catgagttgc gaaatggaga ttgatccaaa
     3301 tggacaagta gtctctcatg atattttccc aagtgtgatc caaacaacag aacggatgac
     3361 ctacacagct gtcaatcaaa tcttagaaga tcaagacgaa gaaacgatgc aacgctatgc
     3421 tcagctcgta cctatgttcc aagaaatggg tgaattgcat cgaatccttg aaacgatgcg
     3481 aatcaaacgt ggcgcgattt cttttgagga tcgcgaagcg aaaatccttg ttgatgctga
     3541 aggacaccca aatgacatcc agctaagaag cagaggaatt ggtgaacgct tgatcgaatc
     3601 tttcatgctt gcggccaatg aaacggtagc tgaacattat aataaattaa atctgccgtt
     3661 tatttaccga atccatgaac agccaaaaga agaaaaaatg cagcgcttct ttgattttgc
     3721 ttcagctttg ggcattctgg tccgaggaac gaaaggaacg atcacaccaa aagatttaca
     3781 gcatgtgatc gaaggagtag aagacaaacc agaagctgca gttatcaata ctatgttgct
     3841 aagaagcatg caacaagcac gctattcaga agacaatttt gggcattatg gcttagcggc
     3901 agattactac acacacttta catctcctat tcgacgctat cccgatctaa tcgtccatcg
     3961 tctgatccgt tcttacagtg aagataaaag tgaaagcaat caaagttcgt gggctgcgga
     4021 acttcctgaa attgcagatc atagttctaa gatggaacgt cgtgcagtag aaacagaacg
     4081 tgaagtagat tccatgaaga aagcagaatt catgatggac aaagtgggag aagaattcga
     4141 tgggatcatc agctctgttg tgaaattcgg cctgtttatc gaactgccga atacagtaga
     4201 aggattgatc catatcaatg aattgaaaca ggactacttc cattttgttg aaaatcattt
     4261 agctcttgta ggtgaaagaa ctggtctgac ttttaaaatc ggacaaaaag tgcgcgtaaa
     4321 agtagtcaaa gctgatccag atgaacgagc agtggatttt gaactcgttt ccgcagaaga
     4381 ggtcactgcg ttggaacgtc caaaagcacg caatggaaaa tctggaaaac gagaaggaaa
     4441 acaagcaggc aaacgctcag aaaaaaatgg caagaagaac aaacgatata gtgatagtaa
     4501 gcaaacgttc ggtaagaaaa aagggaaaaa gccattttac aaagatgtca aaaagaagaa
     4561 aaagaaaaaa taaaatgagt tggaggaaac agctatgcca aaaggcgagg gaaaactaat
     4621 tgcacaaaac aagaaagctc gccatgatta ttcgatcatc gacacgatgg aagcagggat
     4681 ggtcttgcaa ggaacggaga tcaagtcgat acgaaacagc cggatcaatc tgaaagatgg
     4741 atttattcgc gtccgcaacg gggaagcttt cttgcataat gttcatatca gtccttatga
     4801 acaaggaaat atttttaatc atgatccgtt gcgcacgaga aagttattat tacacaagaa
     4861 acaaatcgtt cgtcttgaaa acgaattgaa aaacactgga atcactgttg ttcctttaaa
     4921 agtctatatt cgtaacggct atgccaaggt attgattggt ctggcaaaag ggaaaaaatc
     4981 gtatgataaa cgggaagatt tgaaacgaaa agatatcgat cgacaaattg atcgaacatt
     5041 aaaaaatttc tctagataat ttcattatcc tttttcgcat attcggtgct tttgctttta
     5101 cttaatgata aaatttgtta gcatttaatc tgataaaaca tgattatcac aggttttttc
     5161 ttagatcagg agcaccgtgc atggggaaaa attcgcttcg tttttgactg aaataaaaaa
     5221 gtgtctaatt ttcatgaaaa tgttttgaat tttctgtttc atggtggtat gataccgtgt
     5281 ggttagaaac cgattggaaa aaaccagata cgcacatgaa ttgattttat gtgaatttcg
     5341 gggaaaagag gtgaaatgga ttggatgaac gctcgctaac gtttcacatt ggaccaattt
     5401 ggtttgatgg tacagtttgt ataatggtgt tactgacctg tttgattgtt ttcttcttag
     5461 cttacttttt tacaagaaat cttaagttga aaccaacagg caaacaaaat gcgatagaat
     5521 gggtcattga ttttagtcga ggaatcgtaa cagataatct gcctagaaaa gaattgagca
     5581 atttccattt gttggcattt acattgtttt tatttgtttt tgttgcaaac aacataggtt
     5641 tagtaacaaa aatcgtctta cctaacgaaa caacactttg gaaaagcccg acagcagacc
     5701 cgtttgttac tttaactttg gcatttataa tgatcttact tactcactta ttcggggtga
     5761 aaaagttagg cttcaaagga tatttcgtaa attcgttctt gaaaccctat agttttatgt
     5821 ttccgatgaa actgattgaa gaatttacaa atttattaac acttgcttta cgtctttatg
     5881 ggaatattta tgcgggggaa gttttgctaa ccttgattgc aaaaatgatg acaagcttag
     5941 gctggttctc attaccatta gcaattccat tagagatggt ctggatcgcg ttctccttgt
     6001 ttattgggag catccaagca tttgtctttg tcactttgtc aatggtttat atgagccata
     6061 agattgaagt agaagaataa tactcggtta aaaaacaaac tactatttat ctattttagg
     6121 aggaaaacaa ttatgaatta tatcgcagca gcaatcgcaa tcatgggagc agcaatcgga
     6181 gccggttatg gtaacggtca agttatctct aaaacaatcg aatcaatggc tcgccaacct
     6241 gaaatgtctg gtcaattccg tacaacaatg tttatcgggg tagctctagt tgaagctgtt
     6301 cctatcttag gggtagttat cgccttgatt ctagtttttg ccgtttaatt gaatcagtat
     6361 taaggcagaa acaacatcgt ttgttactgc ttttttacat ggaaactaga aatactccaa
     6421 agaaaggaag gctcgcctat gctgaatcaa ttggcaattg cagaagttgg caatgcgatg
     6481 ttaggtaata tcatcgttgt cagcggctca tttttgatac tactggctct cttgaagcac
     6541 tttgcttggg gaccaatcag cgatattttg aaaaaacgtg aagacaagat cgccaatgat
     6601 ttagattctg cagaacaatc tcgcatcaac tcagcgaaaa tggaacaaga acgcgaacaa
     6661 caattgttag cctctcgttc tgatgcagct gatatcatca aaaatgcgaa agaaagtgga
     6721 gaattaagcc gccaaaatat tttgaaggat gctcaagaag aagcagctcg tctaaaaagc
     6781 aaagcccaag ctgatatcac tgtagaacgt gattcagcgc tgaactctgt aaaagacgac
     6841 gttgcagaac tctctcttca aatcgcggaa aaaatcttga acaaagaatt atctccagaa
     6901 atgcatgaat cattaatcaa tcaatacatt gaaggtctag gttcttcaaa tgaaactaga
     6961 taaatataca gtaggaagac gttacgggaa agctttattt gaactggcga tcgattcaaa
     7021 tcaagcagaa gaaatctatc aagatctcgt aagtttacgt caaatctatg aacaagtacc
     7081 tggattaggg aatatgttaa gtgatgttcg tctagaacct catgaaaaac gagaaatcat
     7141 ggacaagctt gtcagcggtt atgatgggat cgttaaaaac tttttagaag ttgtttatga
     7201 gtataaccga atggatgacc ttttgttcat gatcgaagaa tatgaacgtc gatatgatga
     7261 acatcaaggg ttactattgg gaagtgtcac aacagcagtc ccactttcag acgaacaacg
     7321 aatacaatta gaaaagaatg ttgcaaaaac aatggactat caatcagttg aattaaaaca
     7381 aatcgttgat tcttctatta ttggtggcgc gattgttgaa gccaaccatc gagtaatcga
     7441 cggaagcatt cgcacgcaat tagaaaaaat gcgtaatcaa ttaaacagat agtacaaaga
     7501 ggtaggtgaa tcgaatggcc atcaaagcag aagaaattag tgccttgatt aaagaacaaa
     7561 ttgaaaatta tcaaaacgtg ctttcagtcg aagaaatcgg taccgtaact tatgtaggtg
     7621 acgggatcgc tcgtgcacat ggtttagaaa acgcgatgag tggtgagttg cttgaatttt
     7681 caaacggctc atacggaatg gcgcaaaact tagagtcaaa tgatgtaggt atcattatcc
     7741 ttggcgattt cgagtccatt cgtgaaggag ataaagtaaa acgaacaggt aagatcatgg
     7801 aagttccagt tggagaggcc ttgatcggtc gggtagtcaa tccgctaggt caaccaatcg
     7861 acggactagg tgaaatcgtt actgacaaag ctcgtccagt agaagcgatg gcaccaggtg
     7921 ttatgcaacg taaatctgtt aacgaaccaa tgcaaacagg gctaaaagcg atcgacgccc
     7981 ttgtgccaat cggacgcgga caacgtgaat tagtcatcgg tgaccgtaaa acagggaaaa
     8041 cttctattgc gatcgatacg atcatcaacc aaaaaggtca agatatgatc tgtatctatg
     8101 tagcaatcgg acaaaaagat tctacagttc gtacacaagt tgaaacattg aaaaaatatg
     8161 gcgcaatgga ttacacaatc gttgtgaatg ccggtgcgtc tcaaccagca ccattgcttt
     8221 atatcgcacc atatgctggt actgcaatgg gtgaagaatt catgtacaac ggtaaacatg
     8281 tattgatcat ttttgatgat ctttcaaaac aagctgttgc ttatcgtgaa ctttccttgc
     8341 tacttcgccg tccaccaggt cgtgaagctt atcctgggga tgttttctac ttgcactcac
     8401 gtttattaga acgtgcagca aaattaagtg atgatttagg tggcggttca atgactgcct
     8461 taccatttgt tgaaacacaa gctggagata tctctgctta tatcccaaca aacgttattt
     8521 cgatcactga tggacaaatc ttcttagaaa gcgacttgtt ctacgcaggt actcgtccag
     8581 cggttgatgc cggactatct gtatctcgtg tagggggttc tgcgcaaatc aaagcaatga
     8641 aaaaagttgc tgggacttta cgcctagact tagcaagtta ccgtgaatta gaagcattta
     8701 cacaattcgg ttctgacttg gatgcagcaa cacaagcaaa actaaatcgt ggacgccgta
     8761 cagtagaaat cttgaaacaa aaattgcatg caccgctagc tgttgaaaaa caagtagtga
     8821 ttttgtatgc tttgacacat ggattcttag atagtatccc tgtagacagt attttagact
     8881 tcgaacatga attattcgaa tatctggata ccaatcattc agatatcttt gaaacaatcc
     8941 gtacaacaaa agatcttcca gaagaagaag cgttgaacag tgcgattcag gaatataaag
     9001 acatgttctt agctacaaaa ggaagtaatt cctctacaga agataaattg aaatccattc
     9061 aaaatgcata gtgaggtgag atgaatgggt gcttcattaa atgaaatcaa gacacgtatc
     9121 gcttcaacga aaaaaacgag ccaaatcacg cgggcaatgc agatggtgtc tgcttctaag
     9181 ttgacaaaat ctgaagcttc ttcacagaag tttcaggttt atgcaacaaa agtaagagaa
     9241 atcgttacgc atttgactgc tactcagtta aatgatattg cctcagaaaa tcctagaggg
     9301 gatatcaatt acaacagcat gctgctcagc cgtcctgtga agaaaactgg ttatatcgtc
     9361 atcactgcag atggcggttt agtcggtgga tataacagct cgatattgaa acaaacgatg
     9421 tctattttag aagaagacca ccaatcaccc gatgattacg tgatgatcgc aattggcgga
     9481 actggtgcag acttctttaa agcacgcggt atcaatctag catatgagct gcgcaatcta
     9541 tctgatcagc caagctttga tgaagtccgc aaaatcgtaa atatggcaac aactatgttc
     9601 caaaacgaag tattcgatga attatacgta tgttataacc atcatatcaa ttcgctaact
     9661 agtcagtttc gtgtagaaaa aatgctgcca atctcagact tagatccaga agaagcaacg
     9721 actttcgaac aagaatatat ttttgaacct tcaaaagaag aaatcttgtc tcagttattg
     9781 cctcagtatg ctgaaagttt gatctacggt gcgatcgttg acgccaaaac agcagaacat
     9841 gctgctggta tgacagcaat gaaaacagca actgataatg ctgctaatat cattgatgag
     9901 ctgacggttt cttataaccg tgcaagacaa ggggctatta cccaagaaat taccgaaatt
     9961 gttgccggag cttcggcact agaatagtat tgagattgga ggaaaaaaga tgagttcagg
    10021 caagattgtt caagtaatcg gtcccgttgt cgacgtggaa ttttctttag accaatcctt
    10081 accagatatc aacaacgcat tagttgttta taaaaatgac gaaaataaat caaaagttgt
    10141 tcttgaagca gcgttagaat taggggacgg agtgatccgg accattgcga tggaatcaac
    10201 agatggttta caaagaggaa tggaagtcat tgatacaggc aaagcaatct ctgttcctgt
    10261 aggtaaagaa acattaggtc gcgtattcaa cgtactagga gatacgatcg atttagaaac
    10321 acctttccca gaagatgcgg aaagaagcga aattcataaa aaagcaccag cctttgacga
    10381 attaagtaca agtacagaaa ttttggaaac agggatcaaa gttatcgatt tgcttgcccc
    10441 atatttaaaa ggtgggaaag tcggactatt cggtggtgcc ggtgttggta aaaccgtact
    10501 gatccaagaa ctgatccata atatcgccca agaacatggt ggtatttctg tatttaccgg
    10561 tgtaggtgaa cgtactcgtg aaggtaatga cttgtattat gaaatgaaag attcaggagt
    10621 tatcgaaaaa acagccatgg tgttcggaca aatgaacgaa ccaccaggtg cacgtatgcg
    10681 tgttgctttg actggtttga cgattgcgga atatttccgt gatgtagaag gtcaagatgt
    10741 actgttgttt atcgacaaca ttttccgttt cactcaagct ggatctgaag tatcagcctt
    10801 gttaggacgg atgccttctg cggttggtta tcaaccaaca ttggcaacag aaatgggtca
    10861 attacaagaa cgtatcacat ctacgaaaaa aggttcgatc acatcgatcc aagcaatcta
    10921 tgtgccagcc gatgactaca ctgacccggc gccggcaaca gcattcgccc atttggatgc
    10981 gacaaccaac ttggaacgta aattaacaga acaagggatt tatccagcgg tagatccatt
    11041 ggcttcttct tcaagtgctt tagcacctga aatcgtgggg gaagaacatt ataaagtagc
    11101 aacagaagtc caacacgtat tgcaacgata tcgtgaatta caagatatca ttgctatctt
    11161 aggtatggat gaattatccg atcaagaaaa agtgttagtt tctcgtgccc gcagagtcca
    11221 attcttcttg tcacaaaact ttaacgtggc tgaacaattt actggtcttc caggttcata
    11281 tgtccctgta gaagaaacag tgaaaggatt ccgcgaaatc ttggaaggca aatacgatga
    11341 tcttcctgaa gaagctttcc gaagtgttgg ccgaatcgaa gacgtcgtag aaaaagcgaa
    11401 aacactaggc tactagaaag gggtgcccca tggatcaata tttaacagta aatgtggtaa
    11461 ctcctgccgg tttggtctat gatcaccatg cagctatcgt cgttgcacgt acgacagctg
    11521 gtgagcttgg gattttgcct aaacatgcac caatcatcgt tccattgacg attgatgaag
    11581 ttcgtgtaaa acgaacagat tcagataccc acgtcgattg gatcgctgtt aatggcggaa
    11641 tcatggaagt tcgtgataat gttgtttcaa tcgttgctga tagtgcggaa cgtgaacgtg
    11701 atatcgatgt atctcgtgcg gaacgtgcaa aacaacgcgc agaacgccaa atcgaagaag
    11761 caaaagcaaa acaagatacg aacgaattga aacgggcaac tgttgcgttg caccgagcaa
    11821 tcaatcgtat caaagtatct aagcactcat aaaatgagat gcggatgttt atccgtgttt
    11881 aagaacagta aaaagaggtt atgacagaag tggtcagctt caagacataa gaaggaattt
    11941 acgaaaattg cttctcaaat ttttgtgaaa tctcagctta tttttcgaag aagctacttc
    12001 tggaacaccg tatatcagag gttgtaaaat cagcgttttg tcctgagtat tagagaaaca
    12061 aatggaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagatgat
    12121 tgaacaccgt ttattt
//
LOCUS       DAXWOO010000081        11929 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.081, whole genome shotgun sequence.
ACCESSION   DAXWOO010000081 DAXWOO010000000
VERSION     DAXWOO010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.081"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..258
                     /locus_tag="ACGE70_001722"
     CDS             <1..258
                     /locus_tag="ACGE70_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693358.1"
                     /translation="LKRKVTDRNKNLLNVVMICYISFLILGLPQAVFPEMFSGDWGIV
                     SYSILTLIAMVLGTSFYNAFVSKHVEEYEVLMYLKEKKTSN"
     gene            465..665
                     /locus_tag="ACGE70_001723"
                     /pseudo
     CDS             465..665
                     /locus_tag="ACGE70_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1377..1982)
                     /locus_tag="ACGE70_001724"
     CDS             complement(1377..1982)
                     /locus_tag="ACGE70_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFF8693359.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            2164..2499
                     /locus_tag="ACGE70_001725"
                     /pseudo
     CDS             2164..2499
                     /locus_tag="ACGE70_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387932.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            2664..2879
                     /locus_tag="ACGE70_001726"
                     /pseudo
     CDS             2664..2879
                     /locus_tag="ACGE70_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397514.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3017..3988
                     /locus_tag="ACGE70_001727"
     CDS             3017..3988
                     /locus_tag="ACGE70_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8693360.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            4128..5441
                     /locus_tag="ACGE70_001728"
     CDS             4128..5441
                     /locus_tag="ACGE70_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HFF8693361.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            5484..6350
                     /locus_tag="ACGE70_001729"
     CDS             5484..6350
                     /locus_tag="ACGE70_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8693362.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            6347..7195
                     /locus_tag="ACGE70_001730"
     CDS             6347..7195
                     /locus_tag="ACGE70_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8693363.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            7209..8681
                     /locus_tag="ACGE70_001731"
     CDS             7209..8681
                     /locus_tag="ACGE70_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFF8693364.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            8698..9570
                     /gene="scrK"
                     /locus_tag="ACGE70_001732"
     CDS             8698..9570
                     /gene="scrK"
                     /locus_tag="ACGE70_001732"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFF8693365.1"
                     /translation="MIYGSIEAGGTKFVCAISDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKVAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
     gene            complement(9806..11188)
                     /locus_tag="ACGE70_001733"
                     /pseudo
     CDS             complement(9806..11188)
                     /locus_tag="ACGE70_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            11650..>11929
                     /locus_tag="ACGE70_001734"
     CDS             11650..>11929
                     /locus_tag="ACGE70_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFF8693366.1"
                     /translation="MDSFQRLSLVNQFELLAKFEDQNRSYYERKIEILREGYEYHYDD
                     EIWSDLSEPFPKEDSRFVLDVLNMYRDINFSKSKLNLDENQITNSYYTH"
BASE COUNT         3700 a         2037 c         2401 g         3791 t
ORIGIN      
        1 ttaaaaagaa aagtgacaga taggaataaa aacttattaa atgtagttat gatctgttat
       61 atttcatttc taatactagg tcttccacaa gcagtgtttc ctgaaatgtt ttctggagac
      121 tggggtattg tcagctattc tatattaaca ttgattgcta tggttttagg tacttcattt
      181 tataatgctt ttgtaagtaa gcatgttgag gaatatgaag tattaatgta cttaaaagag
      241 aaaaaaacaa gtaattaata tgagaacacc acgcatagtt tttattttga gatattctag
      301 aatgaaacac attaagaacg aaagcaaagc aaaagacgcc agaacttcta ctaccttcta
      361 agttttttca tatttgtttg ttatgctata catagatctg cataattaga cttgcgctgc
      421 ccgttattga agtttcaggc agcgctcttt ttgggggatc gataatggaa gaagaaaaaa
      481 tatttgagaa acgctggcag ctagcatcaa gtgaacaaag agcaagatac aataatttga
      541 tcacctccta ccccactgtt gactagacat ataaggaaaa gaaatactta ctttggctgt
      601 gccagttaga tatagatacc tttgaaacat ttgaagtgat cctagataaa attaaacaga
      661 attgatcaga aacaagcgaa gccttctctc ggttcgcttg ttttgctatt tattagttgg
      721 ctcagggcaa ctctaagctc gtttgaaaga aaaccgatgg ataatcccct tgtttgtacc
      781 actttagaat acatggatgt acgtattgcc attgactcct attagttact gaaaaaaaat
      841 ttaaacaagt tgagtattgc agataaatag ctccagtgag gtgaataact gctccactca
      901 aggcaataga tagctccatc cagggcaaca aactgctcca ctttcatatt taatagatga
      961 ataaccttct gtataataga aaggcatacc tttcggtatg acaacttata cagaatgtta
     1021 ttttattggt tcactatcga aaaattttgg agctttattt ttccggtatt tctcaacgaa
     1081 cgattagttt aactgtggga agctccagac atacgattcg agaagttact tctcgagcag
     1141 acaggaaggg ggtgactgaa ctaaccgctg atatgagcga tacttggctg taggaatatc
     1201 tttttccgga aaaacaaaca acagcacgag gttaccttcc tgtagactgg gaatatgttc
     1261 ataaagaact aatgaaaaag aatatgacat tgaaactcct tcatagagaa tatggggtac
     1321 atggaacata gaaacgaaaa tgcacggtag aaaagcgaat gaatatagta taaattctac
     1381 aaatcattcg ctttctttat tttatatatg gttggtcgag agagaccatt ttctttggca
     1441 atttctgcaa ctggaactcc ttgatttaac atgtccacca cagttttaaa gactaatcgt
     1501 ttttgcggat ctgttgagtt ctcagaatat tcaattggtc gccccttata tcgaccagct
     1561 tttttggcca ataatactcc ttgccgctga cgttcacgga ttttctcacg ttcgttttgt
     1621 gcaatataac ttagcaatga taaaaacata tcgaacatag cagtatctag gagttcattt
     1681 ccagtattaa aatctaaaaa ctgtgcatct agtattttta atgctacttt tttctgtttc
     1741 ataaaggtaa ctgtattttt tatgtcttca taatctctgc ctaggcgatc cagtgatgca
     1801 acaatgacac aatcaccttc tcggatataa tttagtaatt tttgaaactc aggtcgctgg
     1861 atatctttac cactggcttt ttcttggaat aatttatcaa tgttaaggtc ttccatcatt
     1921 gccaattgtc gatctaaatt ttgatcttgt gaagacactc taatatatcc aatttttgcc
     1981 ataaaaactc cttttaaaaa atgatataat ttacatgtaa aatttacttg tagttttact
     2041 gttatcatag catttttaaa aataaatttt aagtctactt ataatttact ttggaggttt
     2101 tgaatgaatg aaattaagcg caataaaaat tcttttggtg tttatttagg agagcctagc
     2161 tcggaacagt taaataaagg tgaagctcgt catgctctat gtcgaaatat attttatggt
     2221 agaaaaggca aactttatca aacttattac gacggtatgg aggaacagct caacgcatta
     2281 ggattagtaa ctaatgctat catatattgg aattcactat atttagaaaa agtctttgaa
     2341 caaattaaaa cggaagggtt cgactgttcc gaagaagtgt tacgcaagca atttccatta
     2401 attagtgatc acattaattt tgttggcaaa tatagtttcc gctatgaaaa agaattatcg
     2461 aatggaatac tacgtccgtt aaatatagag aattattgag aatgttcgtc ttttggttcc
     2521 taccgtgcat tttcgcacgg atgttccaga tactattttt gaatgtataa aattgaaaac
     2581 acaagaatac taataaatca acgattcttg tgtttttaat ttttcactac cgtgcatttt
     2641 cgtttctatg ttccatgtac cccaatcttg aaaaattaat taaagactta gataaagcca
     2701 ttgacgactt agtcgtggtg ctaccggagt atcaatgttt aaccagtatc ccaggtattg
     2761 gtcctgttta ggctgctgga atactcgctg aaattggtcc aattgaacgt ttttctgatg
     2821 aaataaaatt gacaaagtat gccggcctat attggcgaca gagtcaatcc ggtaaatctg
     2881 aatacgaaaa tttttttacg aaaagcttca cgtctaatat ctattttaaa ataaaaatat
     2941 tcctgctaat ttggaatcgt ttccttcttt tggtataata atacctgaaa ttcttttgag
     3001 aggagttgtt cgtgaagtga aaactactat gaaggatgtt gctgcgttag caggagttgg
     3061 tgtgggtacg gtatctagag taataaatgg agtaaaggtg aaagaggtta ccaaagaaaa
     3121 agtaatggct gcaattagtg agcttaatta tgagcctgat gaatatgcca gaggtatgaa
     3181 aacgaatagt agcaatacaa tagctcttat tttacctacg atttggcatc ccttcttttc
     3241 cgagtttgcc tactatgtag aggaagctct cagccaaaaa aattataagc tttttttgtg
     3301 taatgcgaac ggcagtgcgg aaaaagaaaa agaatatatc caaatggtca agcagagtaa
     3361 agtagacgga attataggta ttacctattc agacattgat aaatatgtat catcaaattt
     3421 accatttgtc agtatagacc gccatttttc ggaacaggtt tcttatgtaa cggcagataa
     3481 ttatttaggt gggcaaattg ccgctcaaga actacttact cgtaactgta aaaacttgtg
     3541 ctatgttggg ggtgtttcac cttaccctaa cgaaacaaac aatcgtaaag ctggttttta
     3601 caatatttgt gaggaaaata aaatagctgt gaaggtattg gatatgcctg aaccaattag
     3661 caatttagag gaacaattgc tcgctttttt ttcggataat cctgaaattg atagcatttt
     3721 taccatcaat gatttcatgg ctttggatgt gataaaagcc caaaaaaaac tagggaagga
     3781 agcacctaag gattttcaaa ttattggttt tgatggagtc aagctatcgt tgcaaaaaga
     3841 ctatttacta tcaacgattg tacagccgat agaggaaatg gccaaggttt cagtagaaac
     3901 actcatggcg atgattttaa atgaagaacc gcctaagcgt gtggtagtac cggttttctt
     3961 tcacgctgga gagacgacta aagagtaaaa aaccggttta taacgatttt tttgagatag
     4021 gttgacagaa agtgatttca atgatatctt aaaaccaatt aattggaaac gttaccaatt
     4081 aattgaaaaa taaaacggaa acgtttcaaa aaataggagg aacatcgatg aaaaagagaa
     4141 tgttagcaat tgcagcaatt ttagcattaa cagctggagg acttgcagga tgtggcagta
     4201 aggagaaaag ttcaggtagc agtgatccag gagaaattaa gatttgggtt cagttttctg
     4261 atgaaacgcc tgaaggaaaa gcttgggcag agattgtgaa caattttaat gatgaatacg
     4321 agggtgaata taaagctgtt actgagtata ttcctcgtag cggaagcggc ggtggatatg
     4381 aggataaagt aaatgctgca attacaacca atacccttcc tgatgttatt acgttagatg
     4441 gaccgaatac tgcagcttat gctcattcag gagtcattgc tccattagat gattatttga
     4501 aagatgcgga catggatgat gtattagaca gcattattca gcaaggaacc tacgatggca
     4561 aaatgtatgc gtttggtttc tcagagtcca gtgtgggtgt ctattacaat aagtcgctgt
     4621 tcaaagatgc tggaattaca gaagatcaat tgccgacgtt agaagatcct tggacttgga
     4681 cggaatttca agcaatttgc caaaagctga ctgataaata caaaacgcct gcaattgata
     4741 tgcaaatggc gtcgggagac gaaatgctga cctacgcata tacaccattt atatggtcga
     4801 atggcggcga tgtcgttaat gctgatggaa ccaaagccga agactatttt aacggtaaaa
     4861 aatccgttga agcgttggaa tttattcaag aattggttga aaaaggttac accacaatca
     4921 gtcctattga aaaaggattt gaaactggca aatatgccat gatgctgagc ggttcttgga
     4981 caatggcaga tttagaggaa aactatcaag agattgatta tggtattttg ccttaccccg
     5041 tttcagatgc aactggggag ttagtttcac caaccggaag ctggcagtta gcaatgactg
     5101 agaaaacaga gaaaaaggat gcttctgcgg cctttattaa gtttgcaacc agtacagaat
     5161 ctagtcgtat tatgagttta gggaacagtg ttctacctat cagaaattca acagttgatt
     5221 taattaaaga tgaagtgtcg gaaggaatgc aggtattaat taagcaaaac caactttctg
     5281 gacatgcccg cccagtggtt gtagcttatc cgcaagtatc tagagcattc caacaagcag
     5341 ttcaagatat tagctacttc gaggaaaacc cagatgtaca aaaagtagca gatagtcgcg
     5401 ctgctgaaat gcaacaggca attgatactt cactaaaata gaagtttcta aagggggcca
     5461 atcaagtgtt aaaaaaaccg acaatgaaag acaatttgat aggttacggc tttctagcgc
     5521 cagctttgct gctgctgacg atttttctga tcatacccgt aatcatggta atttattatg
     5581 cctttactga ttattatttg ttaacgccag atgctagaca atttatcggc ttagaaaatt
     5641 ttaagcgact gttcgctgat ccaatttttg taaaaagtat ttggaacacg gctaaatttg
     5701 ttgtttggat tattccggtc caaatagggg cagctttagg aatggcactt attatcaaca
     5761 aaaatcgtaa aggaaatatc ttttttaaag tagccttttt tgcgccagtc gtgatgtcct
     5821 tagtcgttat ttcgatttta tggttgtact tgttaaatcc taatgaaggt ctggttaata
     5881 cgctatttgc aaagattgga attgcttcgc agcctttttt gactagtccc aaacaagcaa
     5941 tgtatacgct tgttttcgta tcggcatggc aaggtgcagg atatcagatg ctgctgtttt
     6001 tagggggaat gcaaaatatt ccgcaagacg tttatgaggc agccgaaatt gatggttttt
     6061 ctaagtttca gcaatttcgc tatatcacca tgccattatt aaagcccacg gcgattttta
     6121 ttttacttac cacattgatt tcggccttta aactaatcat tcagccaatg gtaatgactc
     6181 aaggtggacc aatgaattct actatgacaa tggtttatta catttaccaa actggattta
     6241 cagaccgtat ggtaggctac tccagctcca ttgcgttaat ctttaccact atgattggtt
     6301 taattacaat tgctcaaaga aaattggtaa aggaggatga tccagcatga ccaagaaatt
     6361 tggaccgcta atgatcgtag aatattgttt gctgttcctt ttagcggcat tattcatttt
     6421 cccgatgctg tggatggtag tttcctctat gaagcctgaa gcagatgtct atcaaaattt
     6481 agcttccttc aaagcttttt tacctagttt gaatccagct aattggttta aaacttatca
     6541 agaggttatt tcccggttca gtgtgggaac ttatttatta aacagtattt tttatggttt
     6601 aacctttgcc tgtgggtcaa tcattgtaaa ctctttggcg ggttttgcct ttgcgaaggt
     6661 taatttttca ggcaagaaaa ttatctttgg agctttgtta gctctgctga ttgtcccgat
     6721 ggagacggtt ttaattccgc aatttaccat cgttaattca ctaggcttag tgaatactcg
     6781 cttggcagtg attttaccag cgatggccag cgcgtttaat atctatctgt ttagaaattt
     6841 ctttatcgcg attccagaag aaatcattga atcagcgaaa ttagatggtg caaatattgt
     6901 taaaattttc tttcacatca tgctgccaat gtctaaacca gccatagcca cagtaggtgt
     6961 cttatccttt atctccagct ggaatgatta tatttggccg ttaatggtct taactgacag
     7021 cagcaaattt tcaatgcaag tggcaattac tacgatcaat actacacagc cagtttatat
     7081 caatcaagtg atggctgttt taaccattgc aacgattcca ctgattattg tttatatcgt
     7141 tgcacaaaaa tatattctgc agggacttgg cggttctgga acgggtataa aataggagtg
     7201 aatagtgaat gttgcaaaaa cttttaaagg ccaatgagtt tattgagaaa aatgctcaaa
     7261 cagtcaatca agaataccgg aataaatttc atttgatggc tccgattggc tggatgaatg
     7321 atccaaatgg ttttgtttat tatcaagagg agtatcactt gttttatcaa tattatcctt
     7381 acgacagcat ctggggacca atgcattggg gccatgctaa aagcaaggat ctaattcact
     7441 gggagcattt gccagtagca ctggcaccag gtgaaagcta tgacaaggaa ggctgttttt
     7501 ccggcagtgc catagaaaag gatggcaagc tgtatttgat ttatacagga cacaatgttg
     7561 tggaagggca agttcgacaa gttcagtgtt tggcagtttc agaagatggc attcactttg
     7621 aaaaatatgc cggaaatcca atcatcgcag aagatcactt agcttctgtt gcaacgaccg
     7681 aagatttccg cgatcccaaa gtgtttcagc gaaaagaaga ctattttact gtagtcgcta
     7741 ctaaaactgc agatgctcgt ggacaaattg tgatgttcca ttccaaagat ttactaactt
     7801 gggagtttta ttccgtattg ctggagggaa ctccagaaca aggcattgta tgggaatgcc
     7861 cagacttgtt tgaactagat ggcaaagaag tcttgattct ttctcctatt caaatgactc
     7921 cacaaggcaa tgcttaccaa aatacgaact caactgttgc tttcatcggt actgtcgatt
     7981 ggcaaacagg caaatttcta gtagagaatt ttcatgaaat cgatggcggt ttagattttt
     8041 atgcacccca gacctgtgaa aacgaagcag gccaaagaat aatgattgct tggatgcaaa
     8101 tgtggcagcg taaaatacct acgaatgatc tgggccatgg ttggtcaggt tccatgacct
     8161 tgcctagaga gctgagggtg gctgataatc aattgttgca acggccagtt tcatcaattt
     8221 atcaagtaat tgatccgcag gaaaggattg aatgggcgaa gactgaagag aatcgcttct
     8281 tactgaagga cttagtatat gaccagcact atttacgctt ggaaatcaat ttaaaacagg
     8341 caacgaaact agaacttcac tatgcgaagg gacccgacca tacattactg cttaactatg
     8401 atgttgaacg ggagtgcttt actgcttcca gggaacacat gccgtatgat ttatcaggag
     8461 ctgaaaagga agtgttgaaa aagcgcagtg tgacgattcc tttggaaaat aattgtctaa
     8521 ccatcgaaat tttccgtgat acttcagcta ttgagctttt tctaaatgat cgtctgacta
     8581 tgagtacaac tttttatgag acagaaaaag gacaggattt tcttttgctg gcagacagtg
     8641 aagtaacctt ttcattggaa agcggcttaa ttaatctata gaagggagag ctcagtgatg
     8701 atttatggtt caatcgaagc aggcggcaca aaatttgtgt gtgccattag cgatgaagaa
     8761 atgacgatca aggaaagagt gagctttccg acaacgacac ctgaggaaac aataccttta
     8821 gtcattgact tcttcaaaca ataccaagca gatttagcag ggattgggat tggctcattt
     8881 gggcctatcg atatccatcg cgattccgcc acttatgggt acattacctc gaccccgaaa
     8941 gtggcatggc aaaattttga tttcatcggt accatgaaaa aagaatttcc gatcccgatt
     9001 tcttggacaa cagatgtcaa tgcggctgcc tatggtgagt atgtcttcgg cagtggcaaa
     9061 ggattgagca gtgtcgttta ctatacgatc ggtacgggtg ttggcggcgg tgccttacaa
     9121 gaagggcgct ttatcgaagg gtttagtcat ccagaaatgg ggcatatgct cgtcgtccct
     9181 catccaaaag atgactttgc tggcagctgt ccatttcatg gcaactgttt agaagggatg
     9241 gccgctggac ccgccatcga aaaacgctta gggaaaaaag gacaagaggt tgcagaagat
     9301 gatccttatt gggaaatcga agcctcctat attgctcaat gtgcctacaa tacaaccttg
     9361 atgttttctc cagatgtgat catttttggc ggtggtgtga tgaaacaacg ccatatgctg
     9421 caaaacgtcc acgacgcatt tgcaaaattg gtcaatgggt atgtcgaaac acctgagtta
     9481 agcaaatata tcgtgacccc agcattagaa gacaacgctg gaacgctcgg ttgtttggca
     9541 ttggctcgag aagcggtctt acattcataa acgaaaaaag gctcttcgac atttagtgtt
     9601 ggtagagaaa gatacaactg aaaatttagt cataaccagt taggcagagt gcttaactgg
     9661 tttttgtttt acttggaaga tcaagccttg aatgagataa gttcttgctc gaaagttatg
     9721 gaaatttcga tagccgtagg ctacttgtat tgtgcaatag ttttttacaa aaaaaagggg
     9781 gtgcggatat tttttttgga cctttttaac aaatatatct ttgcttcttc cgatattgaa
     9841 gagggcttag ccttcctaaa gataacttaa ttcgttgatt attataccaa tgaatataat
     9901 catccaattt gtttataaac tgattgattg ttgtattctt ccagtcacgt tgataaaaca
     9961 tctcgttttt taatcgtcca aagaaacctt cgcacgctga attatctggt gaataactct
    10021 ttttagacat agagtgaagc aagttggctt tgttccaata gtccaactaa caatagcacc
    10081 atcgtaacaa tcgacaagtg gtgataagta gattttccct gcgagaattc taaattcagt
    10141 aatatctgtt aaccattttt catttggctt ctttgcttta aaattccgat ttaataaatt
    10201 ggggtgtgca ggagaaattt ccccagcata cgaagaatac ttttttcgtc tagaaaattt
    10261 aacaaataga ttttcctcag tcatcaatct acgtacaact ttttcagaaa gctttttacc
    10321 catatttttt aaagcttggt gaattctacg gtaaccatac gcacagaagt tatgattgaa
    10381 aatcgtgata attaatttga cgttctttgt aatacttatc tggtttctca agtgcttgtt
    10441 tttgataaaa atagctgctt ttaggaaact ggagctgttg aagaagctct ttcaaggtga
    10501 actgatttcg taaggcatca atcaatatcg ttttttcttg atttgataga caaagaagat
    10561 tgacgccttt ttcttttttt agcagttcag ccgctccttc gagtaatgct ttttcgagct
    10621 gtaattggtg tacttgtttt tgtaagtctt ctacttcctt taaaagtagc tctttttgtt
    10681 tttcctgatt ttttaaagtc atacacaatg gaaaatcctt tccaagaacc gtgtttttcc
    10741 attgatatag agtaactcta ctaattcctc cagattcggc taaatttttc ctattagttt
    10801 gcggtttata gaattttttc aacacagctt ctttttgatc ttgtgttaac tttaccgcac
    10861 tttttcggag ttttctagct cctggtgcaa gttcttcaca ccgctgccgc aaggactctc
    10921 tattaggata accaagcatg cgaatggttc gtgcataaca ttgaccatat tcaaaataat
    10981 gattcaccgc tattcgtttt tgttcttcag aatactttga tttttttcgg tgctcctgat
    11041 ggagctctcc ggattccaaa tactctttat accatttccg tagtgcacca atcgacggat
    11101 accctaattc tcgaatggtt gcagcataag agcaatcgta ttttaaaaat aactgaattg
    11161 cccttacacg ctctttgtgg gaaaacacac ggacattcct cctaagttta ttttttcgtc
    11221 caggattatg tccgcacccc cttgctttct gatgacttat tctggttctt tgagtgtcaa
    11281 tgcatagatg tgatgtagct agttagaaaa aaattttgaa tactcttttt ttctttcaga
    11341 ctccttttta agtaagattt tgaagtgtga gttacggggt cagaaactta aaagtttttg
    11401 atcctttatt ttcaagtctg atttggcagc ttttcggcag ctcatttatt tatggtcaga
    11461 aatagatgct gaaattttaa tctattagca aatagtccag aacgtatgaa gaggagtcgg
    11521 atatcatttg tcattaacac cataaatatt tataaaattc gtgttcacct acaattaaat
    11581 ttaatatata atattcgttg taagggctta taattttttg ttaatgaaca agataggaag
    11641 aaacatttaa tggacagttt tcagagattg tcactagtaa atcaatttga gttattggct
    11701 aaatttgagg accaaaacag atcttattat gaaagaaaaa tcgaaatatt gagagaaggt
    11761 tacgagtatc attatgatga tgaaatttgg agtgatcttt ctgagccatt tccgaaagaa
    11821 gattctcgat ttgtattaga tgtattgaac atgtatcgag atatcaattt tagtaaatca
    11881 aagctaaact tagatgaaaa ccaaattact aattcatact atactcatt
//
LOCUS       DAXWOO010000082        11854 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.082, whole genome shotgun sequence.
ACCESSION   DAXWOO010000082 DAXWOO010000000
VERSION     DAXWOO010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11854
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.082"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            256..531
                     /locus_tag="ACGE70_001735"
     CDS             256..531
                     /locus_tag="ACGE70_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693367.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            544..2622
                     /locus_tag="ACGE70_001736"
     CDS             544..2622
                     /locus_tag="ACGE70_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFF8693368.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            2785..4668
                     /locus_tag="ACGE70_001737"
     CDS             2785..4668
                     /locus_tag="ACGE70_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFF8693369.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            4680..5627
                     /locus_tag="ACGE70_001738"
     CDS             4680..5627
                     /locus_tag="ACGE70_001738"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFF8693370.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5647..6174
                     /locus_tag="ACGE70_001739"
     CDS             5647..6174
                     /locus_tag="ACGE70_001739"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFF8693371.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6237..6890)
                     /locus_tag="ACGE70_001740"
     CDS             complement(6237..6890)
                     /locus_tag="ACGE70_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFF8693372.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7022..7864
                     /locus_tag="ACGE70_001741"
     CDS             7022..7864
                     /locus_tag="ACGE70_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFF8693373.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8022..8918
                     /locus_tag="ACGE70_001742"
     CDS             8022..8918
                     /locus_tag="ACGE70_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFF8693374.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8921..9634
                     /locus_tag="ACGE70_001743"
     CDS             8921..9634
                     /locus_tag="ACGE70_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFF8693375.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9654..10172
                     /gene="msrA"
                     /locus_tag="ACGE70_001744"
     CDS             9654..10172
                     /gene="msrA"
                     /locus_tag="ACGE70_001744"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFF8693376.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10169..10396
                     /locus_tag="ACGE70_001745"
     CDS             10169..10396
                     /locus_tag="ACGE70_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFF8693377.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10548..11099
                     /gene="lepB"
                     /locus_tag="ACGE70_001746"
     CDS             10548..11099
                     /gene="lepB"
                     /locus_tag="ACGE70_001746"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFF8693378.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11182..11724)
                     /locus_tag="ACGE70_001747"
     CDS             complement(11182..11724)
                     /locus_tag="ACGE70_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFF8693379.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
BASE COUNT         4137 a         2011 c         2416 g         3290 t
ORIGIN      
        1 ttttgtcgca gccccatttt tttgttgtga taaaagaaca agaaaggcag gaatgggaaa
       61 gtaaacatcc ttttgtcaga aacttttatc tttcttaagt tttacttgat atctgatact
      121 atttggaata gaatgaggcg ttgaaaaatt tatggaaatt tctaactagc aataaacgat
      181 gaaacaatga aatatttttc atccaaaaag aactttgcta taatgatgga agtttgtgta
      241 taggagatct ggaaaatgag agagagtttt gaacaacaaa aaaaactgtt gcatgatcgc
      301 tatggcgccc tttcgatgga tgaccgccgt caaatacttt gtaagttgcg taaaagaaat
      361 atattgatgt atcgtcagtt ggaacgtctg aaacatgatc tgcttcgttt ggagtcaaaa
      421 cgtgtacagt gtgaattaga ggggaaccaa acacaggttg aagtagtcga aaccaaaatt
      481 ttaaagaaaa aagaacaatt tttaaaaatg cttacacaaa ataaaaaata aaggggatgg
      541 acgatggaat ttgctgagtt agttattgta tttgcagtaa cgatcacctt atcgaacata
      601 gctagccgca ttttgccaat gattccagct ccactgatcc aaattttcct tggagtggta
      661 ttaggtctga cagagtgggg acaatcaatc gattttgaac ctgaattgtt cttagttatg
      721 attattgcac cattgttatt tagagaagga gaaaaagcag atatttcttc gattctcaaa
      781 aatttcggga caattttgtt cttggcattt ggaggagtca ttttgaccct tgtcggggta
      841 ggtgcgacgt tatcattcct gttgccaagt gtcccacttg ctgcctgctt tgcttttgga
      901 gcagcgttag gtccgacaga tgcggtagca gtcagttctc tttctggacg agtgaatatc
      961 cctaaaaagg cgatgcatat tttggaagga gaaggtcttt tgaatgatgc atctggtgtt
     1021 actgccttcc agtttgctct tggtgcgctg atcaccggat ctttttcagc agtaaacgcg
     1081 gggatgagtc tagtagtttc tagtattggg ggagctttga tcggcttttt gcttgtctgg
     1141 ttcaaacaaa aaataattca tttgatcgaa aaagcatcag cacaagatgt cacagggtat
     1201 ctgctgatcg aacttttgct tccttttcta gcctatgtat tggcagaggc atttgatgta
     1261 tctggaatca ttgctgcagt ggcagcaggt attctccaag cttctggttt taggaaaatc
     1321 tctgtgtttg atgcagaatt gtcgagcttg tcccacagta catggacaac tattgctttt
     1381 acactgaatg cattagtatt tatattttta ggaatcgaat tgacgcaagt gttctcgcca
     1441 gtatggggag acggtttata tccaaacggt cttttattag cgattatcgt attaatctct
     1501 gtcatgcttt ttgtgatcag attcatttct atcagtcttt tttatgtatt caaagatggc
     1561 agtaaaaagt tcaaaaaaca gttgaacgag atcttgatct taacatttgg cggtgtaaag
     1621 ggaacagtca gtttggctac gatttttatt ttgccatttt caatcaataa tatgatgttt
     1681 taccagcggt ctttgctttt gtttctgacg gcgggtgtca tcttagtgac tttggtcata
     1741 gggatcattg tcttgcctat gctgacggaa acagaggaag cgaaaagcac ggatttgaat
     1801 gctttgatga ttttagaaga ggtcgttgaa gttttgcgaa aagaaataaa ggaaattgat
     1861 cgtaatacaa aagaattttt agccactgaa gcagtgatag aaaattatca agaacggata
     1921 cgtgatctgt acttggaaga tttaaccgat gatgaaaagc aagaagtaca agaaatacaa
     1981 gcactgattt tgtcgatcga acgggatgga ttggatgaaa gttatcgttc gggcaagctt
     2041 tcttctaatg gttatcgttt ttactcccgt tttctttctc gattcgaaca ttctatcaca
     2101 agccagattt tgtcttttat cggcttttgg ttcattgttg tccgtaggtt aatgcgaatc
     2161 gttctccatc cgaaaatgtt ttggcaaaga agatatgctg atcgtcagac atttattagt
     2221 gatgaagata tccaagaaat cagagagaca tatcaaaaaa atactcagtt gattattgaa
     2281 agtttagata acttgactga tatttatgat gaaacgatgg ttcaattttt tatccagcaa
     2341 cgcaaacttg aaggaataaa aatgaactcg gggaatttga tttcttcgtg gatgatccag
     2401 caagatagtc tgtttacgaa aaaaatgcta agaggttatt atcttgaaag aaaaatcatt
     2461 gatgaatacg aagttgctga aaaaatcacg acgttttctg cgaacagcta tcgcaggaat
     2521 atcaatttgt tagaatctta tacgatgaac aaaccatctg ataacttttc tttccgattt
     2581 gcgtttcgag caaagtcaaa aaaaacgagt ccaataaaat aaaaaggtaa aaattttcaa
     2641 gttttataaa aaacaaaaca tatcagaaca ggatcgaatc ggtcctgttc tttttttgag
     2701 aacacaaaaa gaacaaatac caaaaaaatt ttcctgaagt tcactatttt tatgatacaa
     2761 tatccaagat aaggagggaa catgttgaaa gaattaaaag taactgacct caaaaaaact
     2821 tatggtgaaa aagatttgtt tgaccagatt tcttttttgg tccacgaaaa agaccgtatc
     2881 gggttgattg ggacgaacgg gacgggtaaa acaagtctct taaatattat tgccggtatc
     2941 gatagcagcg acggtgatcg gcaaacagtg ttttatccaa ctgattaccg tattggttat
     3001 ctttctcaag cagctgaatt ttccgatgaa ttaactgttc tccaagcagt ctttcaagga
     3061 aacagcccct tgatccaaac tgttcgagca tatgaggaag cattgatcga actaggggaa
     3121 aacggagaag accctgatgt gcaaaaacgt tatgctaaag cagaagaaca aatgaataaa
     3181 gaagatgctt ggactacaga taccaacgca aagattattt tgcaaaaatt aggaatcagt
     3241 gaattagata aaaaaatcag tactttatca ggtggtcaga aaaaacgagt tagtctggcg
     3301 caagtgttga ttgatgaacc cgatttacta ttactagacg aaccaacaaa ccatttagat
     3361 tatgaagcga tcgagtggtt ggaaaactat ttgaaacaat accgtggcgc actattgatg
     3421 gttacccacg atcgttattt tctagataga gtagcaaacc gaatcttcga actttcattt
     3481 ggaaaattat acgagtataa aggaaattat gaagcttacg tcttagaaaa agcagaacgt
     3541 gatcgtgtag aagtagaaca agaagaaaag cgcaagcggc tttataaaca ggaattagct
     3601 tggatgaggg ctggcgtaca ggcacgtggc accaagcagc aagcacggat caaccgtttc
     3661 gaagatctaa aggagaactt gtaccaagta aaccaagaag atgatttaga gctgaaccta
     3721 gcgacacaac gtttagggaa aaaagtactg gaaatcaaag atggcagtta ccgtataaat
     3781 gaccaaacac tgctagaaca tttagattta ttgattcaat ctcgggaaag actaggtatt
     3841 actggtaaaa atggtgcggg aaaatcgaca ttgcttaata ttctagctgg cagaattccc
     3901 ctggatagcg gtacaatgtc tatcggtgaa acggttcatc ttgcgtatta tacacaagaa
     3961 aatgaagaaa tggcaccaga taaacggatg attgcttact tacaagaagc tgctgaagaa
     4021 gcaaaaacag cagatggcag tcaaatcgga gtagcagaat tacttgaacg atttttattt
     4081 ccaagattta tgcatgggac actgataggc aaactttcag gcggagaaaa acgccggtta
     4141 tttttgttga aactgctgat tcagcaacct aatgttttat tattggatga accgaccaat
     4201 gatttggaca tagcaacatt gacgattttg gaagattatt ttagaagttt tccaggtgct
     4261 gtgatcactg tttcccatga ccgttatttc ttggacaagg tagcagataa gttattagta
     4321 ttccaaggaa acggaaaaca agaactttat tacggaaaca tgtccagtta tttactgaag
     4381 caaaaagaga cccagcagcc tgctgaaaaa gcgaaaccaa aaatacagag caaggaacct
     4441 gctgaaaaga agaaattatc ttacatggaa caaaaagaat gggaaacgat tgaagatgag
     4501 attgcagaac ttgaagaaaa aatctcattg ctccaagaag agatgaatca tcaaggagat
     4561 aatttcactc gattacaaga attgcaaaac gacgtatctg aaacagaagc acaacttgaa
     4621 gaaaaaatgg cacggtggga atacttgagt gaatgggtag aagattagga ggaaaaagga
     4681 tggaacaagc gtatcttgac ttaggaagaa aattgttgga agaagggcat cgtaaagaag
     4741 acagaacagg cacaggcaca ttaagcttat tcggatacca aatgcggttt gatctgcaaa
     4801 aagggtttcc tttgttaaca acgaaacgag taccttttgg attgatcaaa agcgaattgc
     4861 tttggttttt aaaaggagat acgaatatcc gctatctatt acaaaataaa aaccatattt
     4921 gggatgaatg ggcatttgaa cgctacatca aaagtccaga ctataatgga ccagatatga
     4981 ctgattttgg ccatagatgt ttgacagatc ctgaattcaa tgaagaatat aaaaagcaga
     5041 gcaagctttt ttgcgagaag atcctaaccg atgattcctt tgctgaaaca tacggcgatc
     5101 ttggacacat ctacggctat caatggcgtc actgggaaac aaaagacggt ggctttatcg
     5161 atcaaatcaa acaagtgatc gaagcaatca aaaagactcc tgattctaga agattgatcg
     5221 tttctgcatg gaatccggaa gatgttccta gcatggcttt gcctccttgt catacgatgt
     5281 ttcaatttta tgttcaagag ggacggctaa gctgccaatt gtatcaacgc agtggggatg
     5341 tgtttctagg cgttccgttc aacattgcaa gttatgcgtt gctgactcac ctaattgcac
     5401 atgaaacagg tttagcggtt ggcgaatttg ttcacacatt aggagatgct catctttata
     5461 tcaatcatat cgaacaaatg aaagaacaat tgagccgaga aatacgcgca tttccgactt
     5521 tagttttgaa tcaggaaaag cagtcagtat ttgattttga gatggaagat atccaattag
     5581 aaggatatga cccacatccg gcgatcaaag ctcctatcgc tgtttagaat cagaaaggat
     5641 gagaaaatgt tagctgcaat ttgggcacag gatgaaaacg gattgattgg aaaagaagac
     5701 cgtcttcctt ggcgtttgcc aaatgatctg aaattcttca aacaaatgac agaagcaaat
     5761 acactagtga tgggacgaaa aacattcgaa gggatgggta gccgtccgtt gccgaatcgt
     5821 cagacgattg ttttgacgag agattcttcc tataaagcag aaggcgtgca tgtcatgcat
     5881 agtgtggaag acgtgcttgc tctggaaaaa gaaacagacg gtatcttttt catcgctgga
     5941 gggtcagctg tttatcaaga atttctgcca ttttgtacga tattataccg tacagtcatc
     6001 catcatgtat tcgatggaga tgcttatttt ccacctgttg actggtctga ttggtcactg
     6061 atcaatacta gtcaaggaga aatggatgaa aaaaatcgtt atccgcatca atttgaaacg
     6121 tatcagcgta atgagaatcc tgtaaaaagc gggacaggcg aattgacgat ataaaaaagc
     6181 tatggataga ctagctgtta tttagagctg tcccatccat agctttattt tttttattaa
     6241 gtaaagaata aaacagagaa gtacataaaa cctgcaccta acataacaaa caaatgccaa
     6301 acaacatgca tatagcggat agaattcaag ctataaaaga atgcgcctat cgtgtaagaa
     6361 agcccgcctg ctaccatcaa aataacacca gtcattccaa gagagtgata aagaggaaca
     6421 gcagccacga cacagagcca ccccataaaa atatagatga tcgtagatac ttttgatact
     6481 ctctcccgtt tatgcaacgt caacgcttta tatataatgc caagaatcgc aaacaaccag
     6541 attaatacaa ataaagtcca acctagccat cctcggatac tcagcaaaca aaaaggagta
     6601 taacttcctg caatcaaaag aaaaatcgaa ctatgatcga atacttggaa cacctttttt
     6661 gcttttgtaa aaatcaatga atgaaacaaa gtagacgtta aaaataaaag aatcatcatc
     6721 gatccataga tagcataaga gacaatatgg agggaggaat gcaagtgggc accttttact
     6781 aaaagaacca ctagtcccgc gatactcaat ccagtcccaa ttccatgggt gactgcatta
     6841 aacacttcat ttaatatttc atacgttcga ttaaatttcg ttttttccat gaaaacggct
     6901 cctttactag gtgtaattaa tgaaaaatca ttatttgtct gttatactaa aaatagaaat
     6961 tgtcttacat gtagtttaac atgaataagg catcttgtta aagaaagaga gagagaaaat
     7021 aatgacaaat gtaaaaatcg taacagattc ttcatgtacg atggaaaaaa atgtacgcga
     7081 tgctttaggc attcgtgtca tgccgctatc catcatgatc gacggaacag tatatcccga
     7141 tgatgaccaa ctagacggcg aaagatttat ggaaatgatg gcgaaagcaa aaaatctgcc
     7201 caaaacaagc caaccaccaa ttggagaatt catctcgatg tatgatgagc taggagaaga
     7261 cggcagtgaa gtgatttcga tccatatgac aaaaggcttg agtggaacag tagaagcagc
     7321 aagacaagcc agtaatctaa caaaaacgaa agtaacggtt atcgacagtg ataccactga
     7381 ccaaggatta tcttttcagg taattcgagc agctgaatta gcaaaagaag gtaaaacagt
     7441 agagaaaatc cttgaagaag tcaaaaaagt acaagacaat acaaaattat acatcggaat
     7501 atccacacta gataacttgg taaaaggtgg aagaatcagc cgaacaactg gtttgctgtc
     7561 tagtgttttg aacatacgcg tggtgatgaa tttcgcccat tctgaattaa tccctgtaac
     7621 taaggggcga ggaaagaaaa cgttcgataa atggtttaat aatttgaaaa atgaactgaa
     7681 agaattgcca aatgtgcgac aaatcggtat ttcacatgcg gatgcaaaag aattggcgca
     7741 agaattcaaa gagggtttgc aagaattatt cccggacatg catatccctg tactgcatac
     7801 gaatccggtt atcgctacgc atactggtaa agacgcgttt gcaatcatgt attatgtaga
     7861 ttagtcgaaa agaggataag ccctcagcct gaaaggcttg cagcttattc tctttttctt
     7921 tataaaatta ggaaaataat tgttttcttt tttcataaag actacctttt tgataaaatt
     7981 tttgataaca ttagaaccaa tcgaatacaa gaggtgaaaa aatgaaatta acgaaacgac
     8041 atttgatcct cgttttgttc ccattattaa ttgcgatcat catctatgca ggtttagctg
     8101 cttctatacc gaaagctggc ccactaatga aaccagtaac tactgtgaga gaagatccaa
     8161 ataaaaaaga aaaacttcac tacatagcta tcggagattc gctgactgaa ggagtaggag
     8221 atcaaaccaa gcaaggagga ttcgttcccc ttgtcgcaaa tgatctaaaa gaccgttata
     8281 atttgaccgc ggttgaaaca gaaaactatg gtgtaaatgg tgaacgaagt gaccagatct
     8341 taaagcgagt aaaaacaaaa gaagaaattc gaaacaatat caaaacagct gattttatca
     8401 cactgactgt tggcggtaat gatttgatga aagttatcca aaataattta ttcgggttaa
     8461 cggtcaattc ctttaaaaag ccactgaaaa actatcagga aaatgtcaca gaactattgg
     8521 aagaatttcg atccctcaat aaaaccgcac ctatttacat tttaggcatc tacaatccat
     8581 tttatttgaa ctttcctgag atcactgaca tgcagacaat tgttaacaat tggaacaaag
     8641 gaacagaaac ggtgacaaaa aaaatgccga actgctactt tatcccaatc aatgatttgc
     8701 tttatcaagg attgaatcag gaagtaggtg tcacagaaga aggaacagat caaacaacta
     8761 gcacgacaga aaatctaaat ggcatcaaaa ataatgtgct ctatgaagaa gatcatttcc
     8821 atccaaataa tttaggttat caattgatgg caaatgcagt gagagatcag ctgatcgaga
     8881 cacaaaacca atggttagag aaggaggatg atcaataaac atgaaaacaa gatctgacgt
     8941 atcttcaaac aaaacaaaga aaacaaatta ctggaaattc gcattcctta tccttcttgg
     9001 ggtagtactt ggttcgaccg tatttttagg cagccgtatt tttgctaatc gggaaccaga
     9061 actcccagaa ataccagctc ttacggaaag acaaggagat cctgtcttaa cgatcaattc
     9121 aaataaagaa aaagtcaatc aaatcatcag ctttttcctt agtgagtatc aaaaagatag
     9181 tgatattgaa tataaatttt atttggaaaa tgaagcacta ctcaatggta ctttcgaagt
     9241 actaggtttt cctatcgatt tctatttata ttttgatccg tatgtgatgg aaaacggaaa
     9301 tgtccaacta aaagctagaa gtctttctat cggaacattg agtttgccaa tcaaagatgt
     9361 gatgaatatg atcaagagaa actataaatt accagattgg atcgaagtca atacagatga
     9421 tctgactatc atgcttaggt tggatcaatt ccgaatgcaa aacgggatgt atattaaagc
     9481 agataaaatc gacttagtaa atgatgacat tcggtttagt ctatatttgc caaaagatac
     9541 ctctgatgct tcaaacaact caaataccaa caaaactagc accagcagta ctgaaagcaa
     9601 gacaaaagaa aataaaaaat catcggatga gtagtattag agggggaatc ataatggaac
     9661 gagcaatatt tgcaggaggc tgcttctggt gtatgatcca gcctttcgac acgcttcctg
     9721 gtatccatac agtgatgtca ggatatactg gagggcatgt accagatccg acttatgaac
     9781 aagtaaaaag ccaaacgact ggacatacag aagcagtgga gattatctat gatccaacat
     9841 tgatttcata tgaacaatta gtagaacttt actggcagca aactgatcca actgacgctt
     9901 tcggacaatt cgaggatcgc ggtgacaatt atcgcccggt gatcttttat ttgacagaag
     9961 aacagcggat cattgctgaa aaaagtaaga aacagttagc tgaaagtgga cgtttcgaac
    10021 agccaatcgt tacaaaaatc gaaccagccc aaccatttta tctagcagaa gaagaccatc
    10081 aagagtatta taaaaagcat cctgaaaatt ttaaaagaaa ccatgcacgc cgggctgcct
    10141 ttatcgaaga aaattggagg aacgaagggt gaatcgaagc ttttttcatt atttaatgac
    10201 actgcgaggc ggtggcacac atgatgcttt atctgttttc gctacagaag ccggtaaaga
    10261 catccaattt cctaagcaca gctctagcta cgaagaaatt tctaattatc tggaactgaa
    10321 tgtcgactat ttgccgtcca tggatatctt tgacgaagca tgggaaaaat atttagaaaa
    10381 taatcatgac atataagaga tagttttgta ctttatgatc aaaaaagttt tttgtccaat
    10441 agcctaattg aaagaattat ttttatttca gctatctagt cgcatttttt tgatattttt
    10501 cttataataa agaaagaatg aaaaaaagag ggatggattg atagagattg acaaaaaaac
    10561 aacgctatat taatcgcttc tggcttgtat tcaaatactt acttgtttct gtattccttg
    10621 cgttcatgtt gcggggattt ttattcatcc cggtaccagt ggaaggcaat tcaatggaaa
    10681 acgtgttgaa gcaaggtgat atggttgtga tggaaaaatt ttcggagatc agacgatttg
    10741 atattgttgt ttttcaatta gcagatggca cgatttatat caaacgagta atcggtcttc
    10801 caggagaaaa tgtcagctac caaaatgatc aattgaagat caatgggaaa gtagttaagg
    10861 aaccttatct aactaaaaac ctgaaatcag atcatgcaaa tgcatcctat acgacggact
    10921 ttacactaca agagctaacg ggacaatcca agcttccgga agataattat tttgtacttg
    10981 gagataatcg aagagtttca aaagatagtc gttcgtttgg cacgatcaat aaaactgata
    11041 ttctaggtaa agcaagattt gtctattatc cacttgatga aataaaatgg atccagtgag
    11101 aaacaataaa gcgtgcagtc tgttgagact gcacgcttta ttgtttaatc caaaatcagt
    11161 ttactacctt agtacgtaaa atcaaacagc tcgtttaaac acttttctag cctctaaagt
    11221 tttgataaat gaataaacaa ccattatttg aatagggatt tggatcatat tcctgatgaa
    11281 tctacctaga aaaattactt tgaaatctgc accaaacaaa acagacatca gccaagaatt
    11341 gataaaaagg ctgactggga tcataatcaa taattgggca gcgatgacgg aaggcaggtt
    11401 tatcttttta tgatataaaa ataagccatt aataaatcca ccaaggaaag ccgtgataag
    11461 aaaagcagct tgaaacgttc ctgaaccaaa tatcgtgaaa tttaaagtgt tcgacaacat
    11521 ttcaagcaaa ccagcccaga tcggtccata tagataaccc ataagcgcca tacttaaaaa
    11581 tccgaatgtg atttgaaaat aagcaggacc aatcgctatc ttgtataaga tgacatcaag
    11641 ggctaataaa agagcagagg tcgacaacaa tcttgcattc atttcgggca atctaaaaac
    11701 gtcttttgca atcaaagtgt tcaaaatatt ctctcctttg tcagttaata ataaaaggat
    11761 accataaaat caccaatgtg tcatgacaca ttggtgattt acatacactg gcatctaaca
    11821 attaaaaaaa gggtgagaag actgctttta taaa
//
LOCUS       DAXWOO010000083        11841 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.083, whole genome shotgun sequence.
ACCESSION   DAXWOO010000083 DAXWOO010000000
VERSION     DAXWOO010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11841
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.083"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(49..414)
                     /locus_tag="ACGE70_001748"
     CDS             complement(49..414)
                     /locus_tag="ACGE70_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693380.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            622..1104
                     /locus_tag="ACGE70_001749"
     CDS             622..1104
                     /locus_tag="ACGE70_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693381.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     LVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1301..2191
                     /locus_tag="ACGE70_001750"
     CDS             1301..2191
                     /locus_tag="ACGE70_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HFF8693382.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            2517..4991
                     /locus_tag="ACGE70_001751"
     CDS             2517..4991
                     /locus_tag="ACGE70_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFF8693383.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLLKRRNNTVAMTGDGVNDI
                     LAMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLV
                     KNIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNV
                     MEKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKF
                     RSQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLF
                     ERKNEKEQKPFSRVVKKFLSIFKREN"
     gene            complement(5094..6830)
                     /locus_tag="ACGE70_001752"
     CDS             complement(5094..6830)
                     /locus_tag="ACGE70_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFF8693384.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNGDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRAYTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLHGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(6827..7531)
                     /locus_tag="ACGE70_001753"
     CDS             complement(6827..7531)
                     /locus_tag="ACGE70_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFF8693385.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            7662..8165
                     /locus_tag="ACGE70_001754"
     CDS             7662..8165
                     /locus_tag="ACGE70_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFF8693386.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            8125..8463
                     /locus_tag="ACGE70_001755"
     CDS             8125..8463
                     /locus_tag="ACGE70_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFF8693387.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            8484..9902
                     /gene="ffh"
                     /locus_tag="ACGE70_001756"
     CDS             8484..9902
                     /gene="ffh"
                     /locus_tag="ACGE70_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFF8693388.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            10014..10592
                     /locus_tag="ACGE70_001757"
     CDS             10014..10592
                     /locus_tag="ACGE70_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFF8693389.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            10596..11117
                     /locus_tag="ACGE70_001758"
     CDS             10596..11117
                     /locus_tag="ACGE70_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFF8693390.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(11196..11750)
                     /locus_tag="ACGE70_001759"
     CDS             complement(11196..11750)
                     /locus_tag="ACGE70_001759"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFF8693391.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
BASE COUNT         3881 a         2146 c         2446 g         3368 t
ORIGIN      
        1 gtataaaaag aagaaagtag cttctctttg tacatatatg gtttgttttt atggatgccg
       61 agctttttta taagataagg ctaccgccca taacgaccca aggatttgtg gaactaagta
      121 aatacctgca agaagcaaga tcagctgggt cggttttaaa ggatgataga agtaacgcca
      181 ttcagtaaaa ttggcgcaaa ataaatagga agtgacagta gatataatat tggcaatgaa
      241 taaagatata atacgcctgt tcctttgaaa ataatatcca gcaaaaagag ataaaaatat
      301 caatgtgatg aacccgctta cggaatgata acgaaaatca agcagtaagg cgaaatgcac
      361 ataaggaaca aaagataaaa gcaacggaag gtttttccag caagttttta tcatctttca
      421 tcaccaccta acaagtgtta ttattttgtt agttttattg taaattgaat cctgcaataa
      481 tcaattactt tttcattatt tctagattaa atgaataaac tctcatgcca ttatctctaa
      541 aaaaaaagtt tttcccccta atttttttag atagaaaatt gtatagtatg atgagagaaa
      601 aagtagtttg ggggaacgtt aatgagaaga aaagcagtaa tcgatcggct ttatttattt
      661 ttttggtttt ttcctatcgt tttgctacct tttacatggg taaatgctgt tgtacaaggt
      721 gcggagatat taggcctgat ccaaggaagc ggattagtgg tttctaggaa ttttccagct
      781 ttgactgttt tctatttagt gataagtgta ggtctcttat taccaaaatc tctgatcaat
      841 cactacaatt ggcagctgct ttacttttct tgcttattag tatttacatc tatgttttct
      901 ttgctgcctc gcttgattat cggccctaat ggtttgagcg agagtggatt gaaggattca
      961 gcttattcct ccatgtattt cttgacgatc aaaccatggt tctatttagt aattgcctca
     1021 tttattcttg caacattttt ttacttctgg acagaaatca ggataaaaag tgaacgatct
     1081 gtatctataa aaaataacta ttgatggtgg atcatcagta aaaaagcata tgagctgcgt
     1141 aaaatgcatg tttctcatat gcttttttta ttattatgta aagaatgcga aaaacgttta
     1201 catgattttt acataactta actatatttt tacagcaacc atacgaggga tttacagtcg
     1261 actgataaac tttagttgta aataagaaag gagcaataaa atgaaaaaag tattgtttac
     1321 tctaggtatg atgggaattc ttttagccgg atgcggctca ggatcaggaa atacagatgg
     1381 ctctgccaca caaaataatt caagtgactc aaatgaacaa gtaaaaatcg ttgcggtagg
     1441 ttcgacagca ttacagccgt tagtcgatgc agcacaagaa agctttgtac aagaaaatcc
     1501 aaattatcaa atctctgtac aaggaggagg aagcggaact ggtttgagcc aagtcgaagc
     1561 tggagcagtt actatcggaa actctgatgt atttgcagaa gaaagagacg gagttgatgc
     1621 ttcaaaatta gtggatcata aagtagcagt ggtcgggatg gcaccaatcg tcaataaaga
     1681 tacagatgtc aaagatatca ccaaacagga attgattgat atctttacag gaaaaatcac
     1741 taactggaag gaagttggcg ggaaagatca aaaaatcaac gtcgtgaacc gtgcaaacgg
     1801 cagcggtacc cgcgcgacat tcgaaaaatg gggactggat ggtgctaccc ctgtgcagtc
     1861 ccaagaacaa gattcatcag gaacagttcg gcagctggta agtcaaacac caggagcaat
     1921 cagctattta gctttctcat atctcgatga ttccacacaa gcactaagta tcgacggtgt
     1981 agaaccaaaa gaagagaatg tagcagataa cagctgggga atctggtcat atgaacatat
     2041 gtacacgaac ggaaaacctt cccctgaagt ccaaaaattc ttagactaca tgatgacaga
     2101 agaaatccaa gaaggacctg tcaaagaatt gggctattta ccaatcacaa tgatggaagt
     2161 agaacgtgat cacgaaggaa atatcaaata actagcagat aaacatgcca cggtagtcgt
     2221 tactattaag gaaaaatcaa ctatgtttcc ccacatatac atttttcctt taattgtgaa
     2281 aaatagttcc tcatattttg ccgttttttg agctaatacc gagggtcagc tgattgaaca
     2341 ccgtttattc gaaaaaaaga gctacgacat agaactgtcg tagctttttt tttgctatat
     2401 gacaggaatg aaaggtgttt gcaagcttaa tgtgaggaac actttccata tctgttctat
     2461 actggatagg agaagtttgg aaatatccag aaaacgaaat caatcgaggt agccacatga
     2521 agaataaatg gataaatcga agtaaaaaaa agaaagtaac acaaagatca tcagataaag
     2581 tcatccgtct ttttccatcg tataaagaag gattaacggc agatcaagtc aaggaacgaa
     2641 tagaaaaagg tgcagcaaat aattcagtcg atcccacatt caaaacaaat cagcaaatcg
     2701 tattggaaaa catatttacg tatttcaatt tgatctttct tatcttagcc attttgcttt
     2761 gtttagtaga atcctataag aacttgactt ttttaccagt tatcatagcc aatacaggaa
     2821 tcgccatttt tcaggaaatt cgatccaaaa agatcttgga cgaactaaat gttttaaata
     2881 cagcaaaagt caatgtcata cgagacggga tagaacaaac aatagatata gaagaccttg
     2941 taatagatga cattgttatt ttagagactg gacatcaaat tccagcagat gcagaggtcg
     3001 tggaagggaa actgcaagtt aatgaggctt tattgacagg cgaagcagat gaaataacca
     3061 aagaaatagg cgatccatta atgtccggca gtttcattgt ttccggaaaa gcttacgccc
     3121 gattagacaa agttggagca gattcgtata tttcccagct gacagtcaaa gccaagacaa
     3181 tgggagaagg agaacagtcg gagatgattg cttctcttaa tcaattgatc aaatggattg
     3241 ggatcatcat tattcctatc ggtatcctgc ttttttctca aagttacttt tttaatggaa
     3301 acacgatcaa agaaagtgtc gttgcaatgg aagcagcatt gatcggtatg atacctgaag
     3361 gactgtactt gctgacgacg attgcattag cattaagtgc gactaaacta gcaaagcaaa
     3421 gagttctttt acacaatatg aaaagcattg agacattggc aagggtaaat gtcctttgtg
     3481 tagataaaac aggaacgatc acagaaaata aaatgtctgt tcaaaaagtg atcgtctcta
     3541 aaaaacaaga aataatcagt cagtcagatc aattggaagc atgcattgct gactatgcaa
     3601 aagcgatggc aggagacaat gcaacaatgg aagctgttaa agcatatttt acaaaaacca
     3661 ctgatcaatc ctatcaccat gttattcctt tttcttctgt ccaaaaattc agcagtgtag
     3721 cgttggcaga caaggtgtat gttttaggag cgcccgaaat ggttttgcgc gatcaattgc
     3781 cggaatatgc ggaagagttt gtttctttcg ctgaaaaagg ctatcgcgtg ttggtctttg
     3841 gtgtgtacga tgggattctt gaagaaccag tattgacaga agcagtcaca ccattaggct
     3901 atattctgat tgctaatcct atcagaaaag aagcaagagc cacatttgat tacttcaaaa
     3961 aacagcaagt agccatcaaa gtgatttctg gagataatcc gctcaccgta tccaatgtag
     4021 ctgtccaagc agggatttca catgctgatc agtacgttga tgcatctaaa ttatcagaag
     4081 aagaatatga agcagctgtc gaaaaatata cagtttttgg acgcgtcaag ccagaacaaa
     4141 agaaaatctt cgttcaattg ctaaagcgtc gtaacaatac agtagctatg acaggtgatg
     4201 gcgtgaatga tattctagcc atgaaagaag cagactgcag tatagcgatg gcttccggaa
     4261 atgaagccgc catgcaggcc tcacaagtgg tcttacttga ttctgacttt tcacgcatgc
     4321 cagaagttgt tgcagaagga cggcgcgttg tgaacaatat tgagcgatca gccagcttgt
     4381 ttttagtgaa gaatattttc tcctttttgc tctcactgtt ttcagtgatc tttgctctga
     4441 cctatccttt agaaccatca caaatcacat tgattagttt gtttactatc ggacttccat
     4501 ctttcttgct tgctctagaa gaaaacaaaa agcgaatcag agggaaattc atcatgaatg
     4561 tgatggaaaa agctgtgcca ggtggactaa cagatatgat cgtagtcggt gcgttggtga
     4621 tctgcggggt gactttgaat ctaaataaaa cagatgtttc tacagcatct accatgcttt
     4681 tgattgcagt agggttcctc gttctctata aaatctgttc accgctgaat aaattcagaa
     4741 gtcagatcat tcttttctgt gctagcggta actttttttc ggtcatcttt ttgcataagc
     4801 tgttttcaat caccggtatc tcagctgtat cacttttgct gttgagcatt ctatttttct
     4861 cggcagattc gatttttagg catttgacca cattggtaga atatttgttt gaacgtaaaa
     4921 atgaaaaaga gcaaaagccg tttagtcgcg tagttaaaaa atttctatcg atatttaaac
     4981 gagaaaattg acgaaaagta tctcaaaaaa gagtaaaaaa gaacaaccac tcgtgtatct
     5041 ttcgaagcag gatgagtcat ccaactttaa aacgcacgag cagttgctct tttttacatt
     5101 ttcggaatgg taacggtgaa agtcgaacct gttccaagat gactgtctac agtaatcgta
     5161 ccatgaagga gttgcacgta gtcttttaca atcgctaacc ctaatccggt cccgcctgaa
     5221 tgccggcttc gcgccttgtc tacgcggtaa aaacgctcaa agatccgttc ctgatcctca
     5281 atgtcgatac ctataccaaa gtcttggaca gaaaacgata gatcatctgt tgcctgatag
     5341 cggataataa tctcgctgtc gctttttgaa tattggacag cattttcaat cagatttttg
     5401 ataattggat aaaggatatc tgttttagta gtaaatggtt cgttctttcc aatcaatcga
     5461 atcgtcaacc gtttcttttc gatgattggc tgataatttt gaacgatttg ctggaaataa
     5521 ggttctgtat aaattgtctg gatctcgtaa gaattctcgc catcttttga cagctggatg
     5581 atttcccgaa tcagtttatc taaccggatc gcatcttttt gcatgatctc aagaaaagaa
     5641 gtcagagttt gcgggtcatc ttttgcacca tccagtaaag tctcggtaaa accaatcagt
     5701 gaagtcacag gagtttttaa ttcgtgagag acgttgccaa cgaaatcctt ttgtagcttt
     5761 tctaaacgtc gcactttcgt taagtcatac gccaccccta gaatctgtcc ggtaccttct
     5821 gaattagtaa agtagcgcaa actcaagtct aatgtctgcc gaccttctgt aagggtgatc
     5881 tcctgatgga tcagcggatg ctcaggagtc acttgatgaa tcagctgaat gagttttggc
     5941 tcttgaatga cttctgtgta ccgctgttca ggttgatagc ttaccacgtt taaatgtagc
     6001 tgcatctttg gattaagcag tagtaaacga gaatcagcat catcaatcag gaatacgcca
     6061 atcatcagtt cgttcagtaa cgtatataat tgctcttctg tggtagtata cgcccgatac
     6121 atcttactca tttgttcgct caacgcattg atcgtctgat acaattcttc ccattgatcg
     6181 ggtgtttgca tgattacttc cgcctgatta ggattttgca ccattttttt tagtacgggc
     6241 agcacggttt tcaatggctg gtttttttga tagatcagat aatagatcat tcctgcaaga
     6301 acaaggaaga aagacagaaa gaagaaaaag acccaacggc gaaaactctc agttctcggt
     6361 aaaaaaccgc ttgtcggttc tgcgatacgt aaaatggctt ctaactggcc actttttttg
     6421 actggtaatg cgacatataa aagatcccca ttcaaagtag tacttttgcg caaagcgcta
     6481 cctaggttcc caccattcaa tacggctttg atttcgggac gggagtttcg ctgttcattc
     6541 aatgtttcat tgtttgtgtc aaataagatc tttccagtag catccattaa agtaatccgt
     6601 tcgttcgact gatggacgaa ttcttccaaa gcagttttgt tttgcgctgc ttggaggttt
     6661 tcgacatcta attgccggat cagcagttgt gctttttttg tcaggtagct ttcttgctgt
     6721 tccacgatct gctgttgaaa ataatgggaa atcaattgcc agccaccaaa aaacaaacta
     6781 actaaaagaa taagaaagaa gagacgttca taaatttttt tccatttcat cgtttgggtt
     6841 cctgaaactt atagccaaat cctctaatag tcaccaagta ctttgggtgt tttggatctc
     6901 gctcgatttt ttctcgcaaa tgactaacgt gtacatctac gattcggctt tgtcctgcga
     6961 agtcaaaatt ccaaatgcgg tctagcaatg tgtctcggtc gatcacacgg tcttttcgtt
     7021 tcatgaaata aacaagtaat tcaaattctt tcggtgttag ttcaatcaga tggtcgtcta
     7081 cggtcacttg ataattggtc aaatcagcag tgatttgtcc aatcgataga tattcaggct
     7141 cagcttcatc ttttgattgc ttacgaggtt caattcgtct gaatattgct ttcatacggg
     7201 ctaatacttc gcgaggacta aatggtttgg tcaaatagtc gtcagcacct atttctaggc
     7261 caatgatccg atccacttgg tcatctttag ccgtcagcat caaaatcggt gtttcatttt
     7321 tttcttgacg cagccgctga gtgatctcca tgccatccat cgttggaagc atcacatcta
     7381 aaatgatgaa atcaaatgat tgttctaatg ctaattctaa tccttcgcct ccatctgtgg
     7441 cactggttac cttgtagcct tctttttcca aattgaacgt caagagcgtt acaatagaag
     7501 gttcatcatc aacaactagt atttttttca ttcaaatctc ctttaaatag gtttcattct
     7561 gtttatattt tagcatagaa tcagtacaaa gggctatttt acaaaagaaa taaatggtat
     7621 aataggaagg cactttgctg aatagaaagg aagagcagtc aatgattgga atcagtgcat
     7681 gtttaggcgg tctggcctgt cgctatgatg gacagaaaaa agaagtacca gaattgatgg
     7741 aactagttga aactggagaa gctgtcatgg tctgtccaga agtgatcggc ggattaccaa
     7801 ttcctagaga tcccgctgaa atcattggtg gaaatggatt cgatgtgtgg aaagatcaag
     7861 caaaagtatg gacgacaagc ggcgaagatg taacggaagc gtacaaaaat ggcgcgatca
     7921 aagcctatca aaaattgcag gaaaaacaca ttgatctagt catcatgaag gaaaaaagcc
     7981 cttcatgcgg aacccattcg atttatgacg ggagtttttc cggaataaaa aaaaatggtg
     8041 ccggagtggc tactgcttat tttattcagc aaaaaatgac tgtgctctct gatgaagaat
     8101 ggctgaaaac tagaggtgag cagaatggaa atcgaaaaga ccaatcgaat gaacgcgctc
     8161 tttgaatttt attcgacctt attgacagaa aaacaaatga actacatgga attatattat
     8221 gcagatgatt tttcattagg tgagatcgcg gaagaatacg aagtaagccg acaagcagta
     8281 tatgacaata tcaagcggac aagcaagatc ttggaggatt atgaaaaaaa acttcatctt
     8341 ttttctaatt atattgttcg agaacaggta ttggaaaata tgactgcata tattacagat
     8401 aagtacccgg aagataaaaa actacttgaa tatgtacagc aaattcaagc aatcgaagaa
     8461 taaataaaag gaatgaagaa aatatggcat ttgaaagctt aactgaacgt ctccagcagg
     8521 cgatgggaaa aataagaaaa aaaggaaaag tatcagaagc tgacgtaaaa gaaatgatgc
     8581 gggaaatccg ccttgcctta ctagaagccg atgttaactt gcaagtagtc aaagatttta
     8641 ctaagcgcgt acgcgaacgt gcggtagggg cagaagtgct ggaaagttta tcacctgcac
     8701 aacaaatcgt aaaaatcgtt gatgaagaat tgacgatcac acttggttct gaaacagcag
     8761 agttaaataa atcaccaaaa ataccaactg ttatcatgat ggctggtctt caaggggcag
     8821 gtaaaacgac ctttactggt aaattagcca attatttaaa gaaaaatgaa aatgctcgtc
     8881 cgctcctaat cgcaggggac gtatatcgac cagctgcgat cgaccaattg aaagtattag
     8941 gtcaacagct agacgttcct gtatttgata tgggaactga agtcagcccg gtagagatcg
     9001 tccgtcaagg gatggagtta gcaaaagaaa agaaaaacga ctatgtattg atcgatacgg
     9061 caggacgtct gcatatcgat gaaacattga tggatgagtt gaaacaaatc aaagaattga
     9121 cgcaaccaaa tgagattttg cttgttgtcg atgccatgac tggtcaagat gccgtcaacg
     9181 tagctgatag cttcaaccag caattaggta ttacaggggt cgtcatcact aaacttgatg
     9241 gggatactcg aggcggggca gcactatcga tccgctcagt gacaggcgca ccaatcaaat
     9301 tcatcggttc tggtgaaaaa ttgactgact tagaagtatt ccacccagat cgtatggcta
     9361 gccgtatcct tggtatgggg gatatgctga cattgattga aaaagcgcag caagactatg
     9421 acgagaaaaa agcggaagaa cttgcacaga aaatgcggga aaattcattc gatttcaatg
     9481 actttattga acagcttgat caagtgatgg gtatgggccc actggaagat ctaatcaaaa
     9541 tgatcccagg aatgaatcaa gtccctggaa tcgaaaacgt caaggtagat ccaaaagatg
     9601 ttgaacgcaa aaaagcgatg gtttattcta tgacgccagc agaaagagca aatcctgatc
     9661 tgttgaaccc aagccgtcgt cgccgaattg ctgccggttc tggaaacagt gttgttgaag
     9721 tgaatcgaat gatcaaacaa ttcaaggaat caagaaaaat gatgcagcaa atgtccaaag
     9781 gggatatgaa catcccaggt atggaccaaa tgtttggttc aggcgtcaaa ggaaaactag
     9841 gcaaaatggc gatgaatcga atgatcaaga aaaataagaa gaaaaagaaa aaacggaaat
     9901 aagatgaaaa aaacagcttc ggtacagact gaaaatgtct actgccggag cttttctatc
     9961 agcaattttt attctttact aatacaatta ctctaaacga agaggtggca cagatgagat
    10021 gtccgtggag tgaacaaaca gaaagtatgc ggcaatatca tgatactgtc tggggtgttc
    10081 ctgaatatga tgaccaaaag ctgttccgaa aattgatgct tgatattaat caagccggat
    10141 tgagctggca gacgatttta aataaaagtg aagcattcga tgaagcatat gatcgattcg
    10201 atattaaaaa agtcgcaaat tatggagaaa caaaaatcag tgaactaatg gaaaacaaag
    10261 gaattattcg taaccgaaga aaaatcgaag cagccgtcca aaacgccaaa gcagtcttga
    10321 aaattcaaga agaaatcggc agtttttcag agtatctttg gtcgttttct aatggaaaaa
    10381 tcgttgattc ttcttatcat tcacaagaag aagtcccaac aacaagtcct ctttcagatg
    10441 aattagcaaa agatctaaag aaaagaggat ttaaatttat cggaagcaca acaatctatg
    10501 cttttttaga agctgtcggg atcatcaatg atcaccttga cagttgtttc cgcaaagaag
    10561 aggtcgtacg tatcggcaga aagggcgaat aaagcatgcg aaaaatcata ttttatggtg
    10621 ctataagttt agatggctat ttggcaacga aaacggatga tcttcaatgg ctttttgaca
    10681 ctccaacagg taagcaaacc acttatgaag ctttttatga aacaatcgat acgaccatca
    10741 tgggcagaaa aacttatcag gaagcaaaaa aatatttgga gaccgaagca atttatccag
    10801 agaaaaagaa tttcgttttt tccacccacg cacaattaca attgccagat gctgaggtcg
    10861 taaacgaaga tcctgttgct tttctaaaaa atctacaaat gacagaagga aaagatatct
    10921 gggttgtggg tggcggtcga ttgatcaaac cgctattgga acatcaaatg attgatgaat
    10981 ggtatatcca gattacccct gtattattag gggacgggat tcctttattc caaaaaggaa
    11041 catatgaagc gcgttttgaa atgctagaca cgacacgatt cggtgaattt atcgaattgc
    11101 attatgtacg aaagtagaag taaataaaat aaaaaaggca actatatgca gaaatgtcct
    11161 gtacatagtt gcctttttta ttaccgttat gctttttact ctgttaattt gttagaaaaa
    11221 tctagatacg catctaaaat cgtttggaag aaacttaatg ttccttcaac gatattacca
    11281 ttttcatcaa ttagattcgc aacgtttccg atatatgctt ccggttgctg caaggttggc
    11341 acattcaaaa agacaagtga ttgtcgtaga tggtggtttg cgccaaagcc gccgatccca
    11401 cctggtgatg cagtaacgac taaacctggt ttatgatccc aaacactgtg tccataaggt
    11461 cttgagccta catctaaagc atttttcaat actgctggca cagaacggtt gtattcagga
    11521 gtaacgaaaa cgacaccgtc tagtcccctc acttcctcac gaaaacgagt ccattcagca
    11581 ggagcctgtt ctggagtttc gagatcctca ttgtaaaatg gcagatcatc gatttttaca
    11641 aatacacttt cgtacccttc aggaagcaat ttcgcaaaag cttctgctac ttttttgttg
    11701 taagaatctt ttcttaaact accaataaaa aatccaattt tttttgtcat acgatttttc
    11761 ctccttcaaa ctatctataa caatcttaca aaaaataagt aactggagca aataaaaaca
    11821 cttactgaca aaagctacat a
//
LOCUS       DAXWOO010000084        11609 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.084, whole genome shotgun sequence.
ACCESSION   DAXWOO010000084 DAXWOO010000000
VERSION     DAXWOO010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.084"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(44..841)
                     /locus_tag="ACGE70_001760"
     CDS             complement(44..841)
                     /locus_tag="ACGE70_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFF8693392.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(838..2022)
                     /locus_tag="ACGE70_001761"
     CDS             complement(838..2022)
                     /locus_tag="ACGE70_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFF8693393.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(2047..3294)
                     /locus_tag="ACGE70_001762"
     CDS             complement(2047..3294)
                     /locus_tag="ACGE70_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFF8693394.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            3509..5254
                     /gene="dnaX"
                     /locus_tag="ACGE70_001763"
     CDS             3509..5254
                     /gene="dnaX"
                     /locus_tag="ACGE70_001763"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFF8693395.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            5274..5588
                     /locus_tag="ACGE70_001764"
     CDS             5274..5588
                     /locus_tag="ACGE70_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFF8693396.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            5613..6209
                     /gene="recR"
                     /locus_tag="ACGE70_001765"
     CDS             5613..6209
                     /gene="recR"
                     /locus_tag="ACGE70_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFF8693397.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            6273..6917
                     /gene="tmk"
                     /locus_tag="ACGE70_001766"
     CDS             6273..6917
                     /gene="tmk"
                     /locus_tag="ACGE70_001766"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFF8693398.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            6932..7261
                     /locus_tag="ACGE70_001767"
     CDS             6932..7261
                     /locus_tag="ACGE70_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFF8693399.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            7275..8213
                     /gene="holB"
                     /locus_tag="ACGE70_001768"
     CDS             7275..8213
                     /gene="holB"
                     /locus_tag="ACGE70_001768"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFF8693400.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            8249..9073
                     /locus_tag="ACGE70_001769"
     CDS             8249..9073
                     /locus_tag="ACGE70_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFF8693401.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            9066..9413
                     /locus_tag="ACGE70_001770"
     CDS             9066..9413
                     /locus_tag="ACGE70_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFF8693402.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            9482..10354
                     /gene="rsmI"
                     /locus_tag="ACGE70_001771"
     CDS             9482..10354
                     /gene="rsmI"
                     /locus_tag="ACGE70_001771"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFF8693403.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            10463..11584
                     /gene="dinB"
                     /locus_tag="ACGE70_001772"
     CDS             10463..11584
                     /gene="dinB"
                     /locus_tag="ACGE70_001772"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFF8693404.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
BASE COUNT         3714 a         2174 c         2512 g         3209 t
ORIGIN      
        1 ttttgtcaca gccctttttt tgcgaacatt catttagcaa tttttagaaa cttctgccgc
       61 caccgccaaa agtcccgcca ccgctagaat gggtagtact tccgccaccg ccgcttccag
      121 gaggtgggct tttggggatt cttcttgtcg tgacaaaaga atttgtcaga cgatcggttt
      181 tttcagttaa tttgatactg gatttttctc ggaatggata tttgtagatg cctgatttca
      241 attgataacg cgaaacagtc acaaaataaa agacaagact aaggataaga gccaaaacaa
      301 cagcaatgat gatttctact gtcgtcaaaa ccttatagcg tgtaattttg ccagtttctt
      361 cgtctacacg ataatgtcct cctggaactc cgtcttccac atatgttgct gatttagaca
      421 ggtaagcttt tgcagcattg taataggcag catttgtcat ctgatcataa acatcatcta
      481 agatcgaatc gattctgcta tcagtcaaat aatcgatcat attaccagaa gtcgaaatgt
      541 agatttcacg ctgtcccata tctaatagca atactgagcc attttgatca tttccgactg
      601 cgtctcttaa gtaattatca gcaaagtctc ttggttcttc tgaattagat gtagtcgtta
      661 cgatcaagac tcgtcctttg attttttcat tcagcttttc tgcctgttgt tccaaagatt
      721 ggatttgatc tgttgtgaat aacccagcct catcatttac cgtagctgta tctgcatgag
      781 tagccaccgg ccagccaaca cttactagca aaagaaagaa taaaaaaaat tttttcatca
      841 tatcaagtaa cctccgatca gaaagaggat cgctagccca gcgaaaatcc caagagcggt
      901 cagtcccaat tttttctggc tgatcggcag gacaccgctg acctttcctg tttgtccatt
      961 cattgcgtag tagtagactt ttttacttga gtcattgctt cgataagtaa caagccatac
     1021 cggcagtaat acgtaattat ttacttcgtt tgtgattgaa gcagaagttc gcacaccggt
     1081 caatgttgta tatccatttg cggtatcttt taacagcttc tctgaatagt cttttaattc
     1141 agattgaatc tgtgatttga ttgcctcata ttcgatatct cttttttctg cttggaaccc
     1201 ggctaaatac tggcttttaa aatcaattgc tttatctaac gggaaaggtt gcacgccttc
     1261 gaccatcttt tgttgcgtat tttttgataa ggcatttttt actagttctt taaaagaaag
     1321 cttgcctgct cttgaaacag caaattgctt cgtttctgtg tattcgatgt cacctatccg
     1381 ccatatgcgg ataactgtac cattcgcctg catggcacca tctacttcag catctgtagc
     1441 ccaataaggg aaatacacac ctgtcaactt atcgatttga tccttattga aaaaagcttt
     1501 gggaacaaac cattttttct tggtccaagc gagaaatctg ttgattgctt cttctttctc
     1561 gacagcaaaa ggaagtactt tatttggcaa gaattctccg cttaaacgtc ccgatagtac
     1621 gacgggatta tggcagtagt aacaataagt cgcagcagtt gtcgcatcag tcacgatctc
     1681 tgcaccacag ttgggacaaa gaaaaagatc tatcgctgcg gtattatctg cagattcgtc
     1741 cgctgtatct gaaaaaccgc ctgcctcgtc aaccgctttg cgctcggctt cattcatatc
     1801 ggcaagctgt tcttgggctg taaaagtaaa atcttcggta ggtgaaggtt cttctttgct
     1861 ttcttcgcca gccatacgcg cttctttttg cttttgctca tattggctga cttcttcttc
     1921 tgtatagata ttcaggcagt attcgcaatg aaatttttgg tctttgggat caaaagtcaa
     1981 cggaccgccg caattaggac atttatgtgt aagaacatcc atcagctgca cacctttcta
     2041 aataaattaa ttaacgggga ttcctttaaa cggtttacca cagtttggac agaatttagg
     2101 gatgccgttg gaaagatcca cgatctcatt gcattcactg catcgcattt gtagcttagg
     2161 ctgaacgttt tctacaggtt tggcagctcc gcagttggaa cagaattttc cggaattttc
     2221 tgtcccgcaa actggacatg tccaagtatc cgcatttcct tgtgcagttt gttgattttg
     2281 tttttctgct tgttgtttga tttgttcttg gttattttga gctgcttgag aaagataaga
     2341 accatttgca ttcattccca tacctacacc cataaagccg gtcattgctc ctgcttcatt
     2401 ttttccggct gcttccatac cacgtgcaat ggaaccttgc acgtatcctt cacgtacgct
     2461 aggatcacct agcatcgccc cttcgttacg catattgatc agtttcacag aatcatcggt
     2521 gtaagaaatg ctggctacag ccacagaaac aatctccatc ccacgtaatt cacgccaaga
     2581 atcatccaat gcagtatcca tgtatttgct taattccaaa ctcttagatg gtacataaga
     2641 aatccgttga ccatctgcag acatctgatt gattgctgat tgtagcgctg ttaggaattc
     2701 agctaaatac tgctcattga tatcattgat ttccacttgt gttttgtttt tagggattgc
     2761 gtttgtataa aataaaatcg ggtcaacgac atgaatagaa taagtgccat gtgcccgtaa
     2821 gaataattct gcgttataga aattgtcaaa gtagttcaac ggcgcgcttg taccgaattt
     2881 gattcccttt atttcttgga gattgatata aaaaacttgt tgtttctgcg gtgtgacgcc
     2941 gccaaatttg aatcggtcaa aagtttcagc aattgcttct ttcagtgttc cattgaacat
     3001 ggaaggagct gcgtcatttt taatggtgta ataaccttcc tcagcggtat aatcgatgat
     3061 ttttccacca tcgaccaaaa gcatcatcat gtttggatag acatggacga ctgttccgtc
     3121 tgttaaaaca tcttctgttc ctttacgatt ggagccacgt ttgtcatctt tgcggacttt
     3181 gacacctttt gtcatgaccg ttgtatcact catattgtct ggttcgatga cttctagcca
     3241 ttgatctgcc agaccgccgc caaccatact tgttgctgct ttgataagac ccataaaaaa
     3301 ttcctcctct gattcagcta gtgccaaaca attattgttt atttattata ccataggaag
     3361 atgtgaaact tctcggacta gagacggaat tgaaatggaa aattgcgtat tgtttaaata
     3421 cctgaaaaag ccgagaagga tagtagtttt tttacctaat atcacgtata ataagagata
     3481 gtgaattttt taaaaaaggg ggatccttat ggcatatcaa gcactttatc gagtctggcg
     3541 ttcgcagcgt ttcgaagaca tcgttggaca aaaagctgtg acacagacac taaaaaatgc
     3601 tattgtttcc cacaaaacct cacatgccta cttgtttaca ggacccagag gaaccggaaa
     3661 gacgagtgca gcaaagattt tcgcaaaagc aatcaactgt ccgaacagta aagacggtga
     3721 gccatgcaac gaatgcgaaa tgtgtcgatc aattactgct ggtacgcaag aagatgtgat
     3781 cgaaatcgat gctgccagca ataacggagt cgaagaaatc cgatttatcc gtgatcgggc
     3841 aaattatgcg cctacaaagg cagcgtacaa agtgtacatt attgatgagg tacatatgct
     3901 ttcaacagga gctttcaatg ccttgttgaa aacattggaa gagccaaaag aaagtgtcat
     3961 attcatctta gcaacgaccg aacctcacaa aataccagct acgatcattt cacggactca
     4021 gcgatttgat tttaaacgga tcaatacggc agatattgtc gaacatcttg aatttatctt
     4081 gaaagaatcg actatcagct atgaaccagc tgcattgaat gtgattgccc gttctgcaga
     4141 aggcgggatg cgggatgcat tgagtattct tgatcaggca atctcgttta gtgaaggaac
     4201 aattacttta gatgatgcca tgcaagtgac aggtagctta actgacgaaa tgatggacag
     4261 ttacttgagt gcttgcgtga aaaaagaagt accagaagca ttggaaacat tgaaccaaat
     4321 tctcgcagcc ggcaaagaat cacgtcgctt tctagaggat atgttacaat actgccgtga
     4381 cttgctgatg tatcagcaag cacctaacct agttgaggaa caatcgagta cgatcagaag
     4441 tacatttaca gaattggcac aaactgtttc agcggatcag ctgttttcaa tgataaagat
     4501 tttgagcgat actcaaaatg aaattcgttt taccaatagt gccgctattt atttggaagt
     4561 cgctacagtc aaacttgcac agctaccgtc tgcttctgtt ccttcagcag atgtagcatt
     4621 tgcagatgat cagatagtaa aacagctaca acaagagatt aaggaactaa gaaacgaaat
     4681 caaggaacta cgcagtaata caggaattga gaatacaaaa gtacagccgg tacagcaaac
     4741 aaatcataaa aaacagacaa gtacctatcg tgtaccgacg gaacgagtat ataaagttct
     4801 gcgggaagcg acaagacagc atctgaacca agtaaaagaa gtatgggaag acttgctgat
     4861 gagtctatct gtcactcagc gagcaatgct taaagcaagc gaaccagttg cagctggacc
     4921 tacaggacta gtgattgcgt ttgattatga gattgtttgt caaagagcag caaatgacga
     4981 agaattgcag ctttcggtac acaacaactt aagccgaatg atcaaagatt atgcgcctga
     5041 tcctgtttat attacaagag aaagctggcc agaacttcga agaaattatt tgacgcaggc
     5101 acaagaagag ggaacagaaa actcttttgc ttctgaagac tcagaaggag aaatcgagtt
     5161 gcttcctcaa gaacaagaag taccagaaga aacacttgta gtaaaagcag aggaactgtt
     5221 tggctccgaa gctgttacag tggtagatga ttaataaaaa ggagtagata attatgatgc
     5281 gtggaatggg caatatgcaa ggcatgatga aacaagtaca aaaaatgcaa aaagaaatgg
     5341 cagaagcaca agagctgcta aataaaacag agtttacagg taaagccacc aatgaattgg
     5401 tcaaagcggt ttttacaggc gatcgtcgca tgaaagatat ccaaatcaat gaagaagtcg
     5461 ttgatcctga tgatatcgag atgcttcagg atcttgtcgt aatggcagtc aatgatgcga
     5521 tcgcacaaat tgatgtggaa actgagaaaa aattagggaa atacgcaaaa ggtttgccag
     5581 gattttagtt ttaaactgaa ggagtaatga atatgcaata tccagaacca attgcgaaac
     5641 tgatcgacag ctacatgaaa cttccaggaa tcggtcagaa aacagcgaca agactcgcct
     5701 tttatacgat cgatatgaaa gatgaagtag taaatgaatt tgctaaatct ttgttaagtg
     5761 taaaacgcga tttgcatttt tgtagtatct gcggaaacat cacagaagaa gatccctgcg
     5821 aaatatgtag aaatccttca cgtgatcgaa gcgttatcct agtggtcgaa gagccgaaag
     5881 atgtaatggc aatggaaaaa atgcgagaat atcacggact ttatcatgtt ctccatggtg
     5941 tgttatcacc gatggaaggg acaggtccag aagacatcaa tatttcttct ctgttgcagc
     6001 ggctgcatga tgcagatgta aaagaagtga ttatcgcaac aaatgcaaca acagagggag
     6061 aagcaacagc tatgtatctc tcacgtctga tcaaacccgc aggtatcaaa gtgactcgtc
     6121 tagcacatgg tctgtctgta ggctctgata tcgagtatgc agatgaagtt actttattga
     6181 aagctgtcga aggaagacgt gagttataaa tttctaatta agtgtttttt cagatacaat
     6241 ataaaagcag cacaagagat gggggcaaca atgtgaacgg actatttatt acaatcgagg
     6301 ggcctgatgg tgcaggaaaa accagcatct tgaatgaact ttaccctctt ttgaaagaag
     6361 tagcaaaaac agaaatcatt caaacaagag aaccaggagg cattccgatt gctgaagaga
     6421 tccgagcagt gatccttgat ccaaaaaatg atcggatgga tgaacgtacg gaagcactgc
     6481 tttatgcagc agcaagaaga caacatctag tcgaaaaggt tctgcctgct ctggcaaaag
     6541 ggaaaatcgt tttatgcgat agatttgtcg acagttctct tgcttatcaa ggtgcaggcc
     6601 gacgcatcgg cgttaccgag attgctcggt tgaatgagtt tgctacagaa ggtactacgc
     6661 cggattttac cctttatctt gacgtagatt cagacaccgg attaagaaga atcaagaaaa
     6721 acagacaaaa tcaaatcgac cgtttagatt cggaaggttt ggaattccac cagcgtgtac
     6781 gtcatgctta cttaaagctg gcagaagaga atccggaaag aatccacaaa gttgatgcaa
     6841 gaaaaagctt tgaagaagtg ctgcaaacaa gttatcatac aattattgaa cagtatccgc
     6901 aattttttga aaactaggaa ggtgaacgtt tatgaaaatt attttagcga ttatccaaga
     6961 caaagacagc aaccgtttat caaatgaatt gattgatgca aatatccgtg ccacaaaact
     7021 atcatcgaca ggcggcttcc ttaaggctgg aaacagtacg tttatcgtgg gtattgaaga
     7081 tgaccgtgta gaagaagcat tggaaatcat caaaaaaacg tgtgaatcaa gaaagcaatt
     7141 tgtatctaca cctgttacac tggatatttc gatggatggg ggcgtccctt atccagtaga
     7201 agtagaagta ggcggtgcca cagtcttcgt cttacctgtt gaaggattcc atcaatttta
     7261 ggaggagctg agctatgaat caagaaataa acttggctca aatgcaaccg atcgtgtatc
     7321 agcagcttca acggagtttt gagcatgggc gtatcgctca tgcttatctt tttgaagggg
     7381 agaaaggaac aggaaaacat gaagtaggta tatggcttgc acagcacctg ttctgtaccc
     7441 aaatgaaaga caatctgcct tgtggaaaat gcaacaattg tcagcggatc gaaaagaaag
     7501 aacatccaga tgttttggtt attgaaccgg aaggacaaac catcaaagtc gatcagatca
     7561 gacgtttgca gacggaattc agtcgcagcg gttacgaatc aagaaaaaaa gtttttttga
     7621 tcaaagaagc tgaaaaaatg aatagcagtg cggcaaacag tttgttgaaa ttcttggaag
     7681 agccccctgg agatttttta gcggtgttag aaacagatgc agtaggaaga attctcccta
     7741 ctattcaatc tcgttgtcaa gttctgcatt ttcaagaact ttcgaaagaa gcactgatcc
     7801 atcgattgca agaagagcag attccattgg aaaaagcaaa acttttggct ttcttaacaa
     7861 acagtttagg aaaagcagtt gaaatctctc aagatgaatg gtttaatgat gctaaggact
     7921 tgatttatca atggttcgtt tacttgcaaa agcaagatac tcaagccttt atctatgtcc
     7981 aaaaaaaatt ggtaaaatca tttaaagaaa aaacacaaca gtttacagga ttatctgtct
     8041 tgatgtttta ttatcaggaa gcacagcgtc aagcagtagc aaacggcgaa tatggcaaag
     8101 tgaaacagat aaaccagata attgaacgga tattgatcgc agagcagaaa ctacgcagca
     8161 atgtcagttt tcaggctgtt gcggaacagt tcgttttgca tacgattttt tagcttgccg
     8221 caaaggatga tgatcaagga ggatggaaat ggtagaagta gtaggtgtcc gatatagaga
     8281 tgcaggtcat atctattatt ttgcaccagg agactctgaa tatgtttata gtgacaaggt
     8341 attagtcgaa tctcagcaat ccttacatat tgctactgtt gtccttccta aaaaagagat
     8401 tgatcccaat gaccttcctg aagctatccg tccgatcttg cataaagcgt cacaagctga
     8461 attagaaaaa gacaaaaaaa acaaaaaaga tgcttatgat gcattgaaaa tcgcaaaaga
     8521 aaaagtgcag ttgcgtgagt tggaaatgaa aatggtcaaa gccgaataca catttgatcg
     8581 aaataaattg attttttctt tcacagcgga cgggcgaatt gactttagag aattggtaaa
     8641 agatctggcc gctgtatttc gtactcgtat tgagcttcga caaattggtg tccgcgatga
     8701 agcaaagata ataggcggaa ttggtccatg tgggcgtcca ttatgctgtt cttctttttt
     8761 aggcgatttc gtgcctgtat caatcaaaat ggcaaaagat caaagtttat ctttgaatcc
     8821 aacaaaaatt tccggtttat gcggacgctt gatgtgctgt ctgcaatatg agagcgaaga
     8881 atacgagaca gcgaaaaaag aactccctga ctttggaaaa gtaattgcta cacctgatgg
     8941 taaagggaaa gtcgtagggt tgaatctgct tagccgtatc gttagtattc ggctgactgg
     9001 acgggaaaat cctgtcgaat atgaatggga tgaattgaaa gaatttatcc aagtaggtga
     9061 gatgaatgga taagagatca ttatacgacg gattgaatca attagaatca gagttgagaa
     9121 atacattaac agaacttgtt gaaatgaagc atgctttgca tgagatcgta gaaaaaaata
     9181 caactttgga gatggaaaat caacgtctgc gggaacacct gcgggaattg aaccaagttg
     9241 cacaagctcc agctgaaaat gtgaagcagg aactttctaa gtctcgtatg aatcttgaaa
     9301 aaatttatga agaaggattc catgtctgct acgatttata tggatcaaga agggacaatg
     9361 acgagccttg tgcgttttgt ctagaagtga tctatcgcga aagcggtcga tgaccaataa
     9421 caagaggaga ctggagcgga gaaatactgc tccagtcttt ttattaagga gattgtgaca
     9481 gatgtataat caaaaaagtt tcaagggaaa tccggtatat ggggatttat atcttgtacc
     9541 gacgccaatt ggaaatttag aagatatgag ctacagaagt gttcgtatgc tgcaagaatc
     9601 cgatcttatc gcaagtgagg atacgcgtaa tacacaaaag ttattgaatc attttgaaat
     9661 ccatacaccc caaaaaagtt tacatgaaca taattataaa gaacgtgtcc ctcaattgat
     9721 tgctttgtta aaagaaggaa aaacgattgc tcaagtcagt gacgcaggaa tgccttccat
     9781 cagcgatcct ggacatgagc tagttttagc ctgcataaaa gaagggattt ctgtgatctc
     9841 tatcccagga cctacagctg ggatgacggc tttgattgct tcagggctag tgcctcaacc
     9901 atttttattt tatggcttcc ttccacgtaa gaaaaaagaa cagaaagaaa cattgaaaaa
     9961 gctaaaagaa cagacagtta cgctgatttt ttatgagtca ccttatcgga tcactgctac
    10021 attgacgaat ttcttagaaa tttttggaaa tcgccaagtc gttttgtgtc gggaattgac
    10081 aaaggtccat gaagaatacc ttagaggaag tacggaagag ctactcgctt atatcgaaga
    10141 acatccagta aagggtgaat gttgtctgct tgtagaaggg aatttgttcg aaatgccagt
    10201 gacggaagtt gatgaagagt tgggaacgtt aaaggaacaa gtagaagcaa agatcgcttc
    10261 aggcgaaaaa ccaaatgctg ctatcaaagc agtagcgctg aaaaatgggc tgaaaaaaca
    10321 agaagtctac cgtcagtacc atgaattaga ctgagatagt ataagttgtc tataatgaaa
    10381 gactaatgag gaaatcagct actaactttt caaatctctt gaacgcgaac gaatgttctt
    10441 gtataatgaa taagaggtga ggatgatgga gctgcaattt cctttacata atgatacatc
    10501 aagaaaaatc attcatattg atatggatgc ttttttcgct tcggttgaag aacgagatca
    10561 tcctgagttt cgcggacatc cattagtaat tgccagacac cccagtgaca ctggtgggcg
    10621 tggagtagtg actacagcaa attatgaagc aagaaaattc ggaattcatt cagcaatgag
    10681 tgcacaaaaa gcgtatgagc tttgtccgca agccatcttt aagcctggcg accatcataa
    10741 atacagcgat atttccaaac aagtacggga agtattctat cgttatacag atattgtgga
    10801 accagttagt atcgatgaag cgtatcttga tgtgaccgaa aataaaatca attgcaagtc
    10861 agccatcaaa atcgcccgaa tgatccaagc agatatttgg gagagtgtcc agctaacatg
    10921 ttctgcggga gtaagttaca ataagttttt ggccaaatta gcatcagact atcaaaaacc
    10981 tagaggaatc acggtagttg cccctcaaga cgctgtgcca tttttaaaag ctttgccaat
    11041 tgaaaaattt catggggtag ggaagaaaac tgtacctaga atgcatgagc tagggattta
    11101 tacaggaaaa gacctatatg aatgcacaga aatgatgttg attcgaaatt ttggaaaaat
    11161 gggctattct ctttatcgta aagtcagagg aatccatgat tctccagtaa acgttacaag
    11221 agagcgaaaa tctgtgggaa aagagcatac ttatgggcag ccgttgcaaa cggaagaagc
    11281 agtgctgact cagttgcgcc aactagctga aaaagtagaa gaagcactga gaagagtcca
    11341 aaaacatggg aaaacagtag ttttgaaggt caggtacacg gactattcta ctgtgaccaa
    11401 aagagtgaca ttgccagaat atatctataa aaaagaagca ctcttttatc aagcaagcct
    11461 tatatgggag gaaatcttgg gagtagaaaa aggaatccga ttgttaggga ttactttgac
    11521 gaatctggac ccgatgactt atgaaaacat cgtccttcca ttatgggaaa atcaagaaat
    11581 ataaaaacag ctttgagagt tgaaaccgg
//
LOCUS       DAXWOO010000085        11553 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.085, whole genome shotgun sequence.
ACCESSION   DAXWOO010000085 DAXWOO010000000
VERSION     DAXWOO010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11553
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.085"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(90..1250)
                     /locus_tag="ACGE70_001773"
     CDS             complement(90..1250)
                     /locus_tag="ACGE70_001773"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFF8693405.1"
                     /translation="MSKGKHRKKQKISISFLVILLLSVSFFSTGIIIGGQLTKIPHSQ
                     TYKAEPTQLTSNLTPDEQRLASKIDQLLQKSDYIGSIYVRKNNRVLLEKGYGYANLRV
                     KESNSPSIYYQIGSIQKAMTALLILKQIQAGKLTFDTKLAEFYPQIPGSQQITIKNML
                     YMRSGLHRTASPDKPMSDSEIIQFALHHLKLTDYDTYHYEPLNYTLLTGILIKLTNQP
                     YETLLKKEIIRPLHLEHTDFYDNVKNSPQHAVSYQMSATDDYSKALIEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGGIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVVAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVHLSN"
     gene            1415..2590
                     /gene="mltG"
                     /locus_tag="ACGE70_001774"
     CDS             1415..2590
                     /gene="mltG"
                     /locus_tag="ACGE70_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFF8693406.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            2726..3205
                     /gene="greA"
                     /locus_tag="ACGE70_001775"
     CDS             2726..3205
                     /gene="greA"
                     /locus_tag="ACGE70_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFF8693407.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            3373..4104
                     /gene="liaF"
                     /locus_tag="ACGE70_001776"
     CDS             3373..4104
                     /gene="liaF"
                     /locus_tag="ACGE70_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFF8693408.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDVEVIRI"
     gene            4101..5168
                     /gene="liaS"
                     /locus_tag="ACGE70_001777"
     CDS             4101..5168
                     /gene="liaS"
                     /locus_tag="ACGE70_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFF8693409.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEITKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILKLERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWEVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVETKVPLMKEA"
     gene            5175..5807
                     /locus_tag="ACGE70_001778"
     CDS             5175..5807
                     /locus_tag="ACGE70_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFF8693410.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDWPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            5947..6612
                     /locus_tag="ACGE70_001779"
     CDS             5947..6612
                     /locus_tag="ACGE70_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFF8693411.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            6640..6933
                     /locus_tag="ACGE70_001780"
     CDS             6640..6933
                     /locus_tag="ACGE70_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFF8693412.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(7001..7948)
                     /locus_tag="ACGE70_001781"
     CDS             complement(7001..7948)
                     /locus_tag="ACGE70_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFF8693413.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(8126..8929)
                     /locus_tag="ACGE70_001782"
     CDS             complement(8126..8929)
                     /locus_tag="ACGE70_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFF8693414.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            9075..9989
                     /locus_tag="ACGE70_001783"
     CDS             9075..9989
                     /locus_tag="ACGE70_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFF8693415.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKNVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10037..10507
                     /locus_tag="ACGE70_001784"
     CDS             10037..10507
                     /locus_tag="ACGE70_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFF8693416.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            10592..11200
                     /locus_tag="ACGE70_001785"
     CDS             10592..11200
                     /locus_tag="ACGE70_001785"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFF8693417.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNAAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            11334..11534
                     /locus_tag="ACGE70_001786"
     CDS             11334..11534
                     /locus_tag="ACGE70_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFF8693418.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
BASE COUNT         3871 a         1794 c         2367 g         3521 t
ORIGIN      
        1 gataatattt gttaatttat ttctactttt cagatagagt actaactaaa ttgactatac
       61 agagatgaca actaatatta tgtcaaaatt taatttgata agtgaacacc ttccatcagt
      121 tcataaatct gatttcttaa atcagtggcc ggcaatttga tgttcttatc cgattggata
      181 ttactttcca tgacgattgc atctttagtg tcttttttca tcgccactac tgcatggaat
      241 cgattgatat tcccctgagc aaggatataa tcgtttccac tgtaaacacc gccgctgtaa
      301 ggatgtttat caccattatt ttgccacaat gagaaaagta gttcttttgg tatgataccg
      361 ccagaaagaa cactggtaaa gaatttgttc aagtcataaa cagacatact tatgtttcct
      421 gtacctaatt catttcggat gtcggtttca ggctcgatca atgcttttga ataatcgtca
      481 gttgcagaca tctggtaaga tacagcatgt tgaggcgaat tttttacatt gtcataaaaa
      541 tctgtatgtt ctagatggag tggacgaatg atttcttttt ttagaagagt ttcataaggc
      601 tgattcgtta gtttgatcaa tatccctgta agcaatgtat aattcaatgg ttcataatga
      661 tacgtatcat aatcagtaag tttcaaatga tgaagggcga actgaatgat ttcactatcc
      721 gacataggtt tgtcgggaga agcagttcga tgaagaccag agcgcatata aagcatattt
      781 ttgattgtga tttgctgact tccaggaatc tgaggataaa attctgctaa ctttgtgtca
      841 aaagttaact taccagcttg gatttgtttt agtatcagta aagcagtcat tgctttttga
      901 atcgaaccga tttgatagta aatagaaggt gaattcgatt cttttacgcg cagatttgca
      961 tatccatatc ctttttctaa tagtacgcga ttatttttgc gaacataaat cgaaccgata
     1021 taatctgatt tttgtagtaa ttgatcaatt ttgctagcaa gcctctgctc atcgggcgtt
     1081 aaattggaag taagttgtgt tggctccgct ttataagttt gggaatgagg tatttttgtt
     1141 aattgacctc ctataatgat gcctgttgaa aagaagctga cagacaataa taaaattaca
     1201 agaaaagaaa tagaaatttt ttgttttttt cgatgttttc cttttgacat gttaattcct
     1261 ttctatccat ttttaactat ttttcattgt aatcctcaaa aagagtattt gccacttaca
     1321 attttgacat aatatttggt aaaataattc acggcagtag aaatttcatg tataataaac
     1381 agagttacga agatgatagt aaggaggagt tgttttgtca ggaaacaaac aacacccaag
     1441 taaagataaa aaagaagcca gtaaggcagc tacagcagat ataatcgaca agcggagaaa
     1501 tctgcgtaaa aaagaagata agattgtgag gaagatcatt ttagtgattg ctcttacatt
     1561 attaattatt ggcggatttc ttggatttac cgtgtatcgt tatgtagata gcgggttgaa
     1621 gcctttagat aaatcagatg accaattagt ccaagtggag atcccgagtg gctcttctaa
     1681 caagcagatt ggtgagattt tggagaagga caatattatt aaaagcggga ttgttttcaa
     1741 ttattataca aaatttaaaa atttaactgg ctttcaagct ggctattatc agttagcgcc
     1801 taatatgact cttgacgaga ttggcaaaca gctgcaagaa ggcggcactt ctgagccaac
     1861 aaaagttgca gatgggaaaa ttgccattcc agaaggctac gacatcgatc agattgctga
     1921 gcgggtagcg aaggtaactg gtaaagacaa gaaagaattt ttagatttag taaatgatga
     1981 gactttcttt aacgagttgt atcaaaaatt cccagaattg ttggaaagtg cctctaaagc
     2041 tgagaacaca aaataccgtt tggaaggcta cttattccca gctacttatg attactacac
     2101 gagtacttcg cttaaggatc ttgtgattga gatggtgaac aaaacaaata cggtcatgca
     2161 aaactattat tcagctatca aacagaaaaa tttgactgta caagaagttt taactttggc
     2221 ttctctagtt gaaaaagaag gcgtaaaaga aaatgatcgt aaaaatattg cacaagtctt
     2281 ctttaaccga atcaaggcaa atatgccact tcagtcagat atctctgttt tatatgcatt
     2341 aggagaacat aaagaattgg tgacgtatga agatacagct gtagattcac catacaattt
     2401 gtataccaat accggctatg ggcctggtcc ttttgacaat ccaagcgaag aggcaatcaa
     2461 ggcagtatta gaaccagcag aaaatgacta ttattatttc gtagcagata cgagcactgg
     2521 aaatgtttat ttcgctaaaa cgtatgaaga acatatggaa ctagtgcaaa aatatgtaaa
     2581 taattcttag aaaaacaggg gctagtcatt gatcattttt agaaaaacat ttacatttta
     2641 agaaaagcgt ggtaatcttt tttggactat attcctcaaa agattacctt ttgcggtatt
     2701 ttatcgaaaa aaggagacgt tacacatggt agagaaagtt tatccaatga ctctagaagg
     2761 taaagaaaaa ttagaaaaag aattagaaga actaaaaaca gttaaacgcg gagaaatcat
     2821 tgaacgaatc aaaatcgctc gcggtttcgg agatctttcc gaaaactccg aatacgaatc
     2881 agctaaagat gaacaagcat ttgttgaagg aagaatcaca acaattgaaa atatgatccg
     2941 ttttgcacaa atcatcgata atgacggcgt agatgcagat gaggtatcga tcggtaaaac
     3001 agttacgttc atcgagttgc ctgatggaga tgaagaagag tacacgattg tcggaagtgc
     3061 tgaagcagac ccgttttctg gaaagatttc caatgattct cctatcgcgc aagcattgat
     3121 tggcaaacgt aaaaatgatg aagtaaccat tgctacacca ggtggcgata tgttagttaa
     3181 aatcgtcaaa gtagaacaag cgtagtctga ctgaaagatc tagagagttc atcttactga
     3241 tgacgcactc tagatctttt ctctttgagt attgcaggtt tcactgaacg atcttacatc
     3301 taaagtccta tagccaacag tcaaaaaaat aggtatgatg aaatgagaat gtgtcaggag
     3361 ggagaaaaag cgatgaatag ttcttggcgt ttttttgtgg tagtcgaagc actgctgctg
     3421 ttatttgctg tttggcaaat cgtaaacaat actggacttt tgcttttagt aatattcggt
     3481 attttcaata tatatctagc tatgcgcaaa tatcctcgta ctaaatttca aaattttcaa
     3541 ttgatcttag ggagcttggt catttttttt agcttagtca acagtcctgc tttatggatg
     3601 atggttgtat tggccgtttt atttatcggg ctgaaaggtg tagagatttc aggaatagat
     3661 ttgacaaaaa atgctttttg gcgaaaaaaa cagatcatga tggtccaaac agagcaatta
     3721 aaaacacata ataatgagcg taaaagacag caattatttg gtaaccaacg tattggaaac
     3781 gacgtctatg aatgggatga tatcaacatt gccatcattt ctggtgatac tatcatcgat
     3841 ttgggcaata cacttttgcc aaaagatgat aatattgtga ttgtaagaaa agggataggc
     3901 agaacacgga tcttagtacc cctgggagtg gctattcgtt tagagcatgc gacattagta
     3961 ggaaatgtct tgtttgaaga agagcagttt tccttaaaaa acgaacagat caaaatctgc
     4021 agcagtgatt atgatgaaaa tcctcgcaga ttgaagatca ttaccaatac tttggttgga
     4081 gatgtcgagg tgatacgaat atgatgggaa gaatatccaa agcaatgcta gctgtttatt
     4141 cggggattgc ggctttcctt attattctat tttcactttt cacttatttt tatgccagta
     4201 accaaagtca ttggtgggga gaattgctgc gtgcgcgttt attatacgtt ccgcttatct
     4261 tccatttgat ggcaatttca ttaggtgtag gattgatcgt atttctattg ttatcactta
     4321 ttcaaaaggc aaaatacggg aaaatcgaag aaaagcttcg tgcactttct tctggcaatt
     4381 atgaatccaa acttttgcat cagccgatcc caagcgcgtc ggatgatctg tacatcaaag
     4441 atattgatat ggaaattact aaaataaaag aaaaaatgat cgaagtatcc agtgaattac
     4501 agattatgac tagccgtcct caatatgtag atggacaaac caaagaagag attttaaaat
     4561 tagaaaggca tcgattagct cgtgagcttc atgattcagt ttcacagcaa ttatttgcag
     4621 cgatgatgat gatgtcggca ttgacggagc aagcagaaaa aagtgagaca ccagaaatgt
     4681 tccgtaagca gttgaaaatg gtggcagaga tcatcaacgc ttcccagtct gagatgcgtg
     4741 cgctgcttct tcatctgcgt ccagtcaatt tagaagaaaa aagtttaaaa caaggcattg
     4801 agcagttatt gaaggaattg cagaataaaa tccagatttc gctaaaatgg gaggtagaag
     4861 atgtaaaatt aactagttcc attgaggatc acttgttccg aatcgttcaa gaattgttat
     4921 caaacacatt aagacatgct aaggctaatg aattagaagt ctatttgcat aaaatagata
     4981 acaatctttt attacgtatc attgacgacg ggacaggatt taatatgaat gaaaccaaaa
     5041 cgggaagtta tggattgaac aatatcaaag aacgagtagc tggtattggt ggaacagtaa
     5101 aaatcattag ttttaaaggg caaggtacga gcgtggagac caaggtccct ttgatgaagg
     5161 aggcatagat aaagatgata aaagttttat tagtagatga ccatgaaatg gtacgcttag
     5221 gcgtctcttc ttatttatct atccaagaag acattgaagt gattggagaa gctgaaaatg
     5281 gacgtcaagg ctatgaaaag gcgatggcac ttcgaccgga tgtcatttta atggatctag
     5341 tcatggaaga aatggatggc atcgaatcaa caaaagcaat cttaaaagat tggccaaaag
     5401 ccaaaatcat tatcgtcaca agttttattg atgatgaaaa agtttatcct gcaattgaag
     5461 caggagcagc aggatatttg ttgaagacgt ctacagcaca tgaaatagct gatgctataa
     5521 gggctacgca acgcggcgag cgagtactag aacctgaagt gacaacaaaa atgatggaaa
     5581 aaatgagtcg gcgaaatgat ccagtattgc atgaagaatt aacgaatcga gaaaatgaaa
     5641 ttttgatgct gatttctgaa gggaaaagta atcaggaaat cgcggatgaa ttgtttatca
     5701 cactgaaaac agtcaaaacg catgtgtcga atatattagc taagttagag gtagaagacc
     5761 gaactcaagc agctatctat gcgtttaaac atggtttggt gaaataaaaa cgatgataaa
     5821 gaggataatc aaaagctgag aaaataacta ttatttttct tggctttttt acaataatga
     5881 tgcttgcttt tgtttttgga aaggctttgt tatactgaga aaagaagata ctaaaggagg
     5941 aaaatggtga aacaaaattt tgcaattgta ggccttggac gatttggcgg aagcatctgc
     6001 cgtacactga tcgaagctgg acaagaagta ctggctattg acagtaatga agatcgagtc
     6061 aatgaatata tgaatatagc cacccatgct gttgtggcaa atgcacagga tgagatgaca
     6121 cttcgttcat taggtataag aaatttcgat catgtaattg tcgctattgg agaagatatc
     6181 caagccagca tcttagttac tctgatggta aaggaaatgg gcgtaccgaa tattttagca
     6241 aaagcccaaa atgagtacca tgcccgtgta ctagagaaga tcggagcaga tcgtgtcgta
     6301 caccctgagc gagacatggg gatacggatt gcacataact tagtatcaaa gaatattcta
     6361 gactacttag agctgtcaga taaatattct ttagcagaaa tccgagtctc caatcctaaa
     6421 tttttcaaca aaacattagc tgaattaaat ttcaggcaac gttttgactt aacagtagtc
     6481 gccatacgaa gagtagatca aacggttatc gcttctcctg cagcagatga atatgttcgt
     6541 gaaaatgata atctactggt tattggagag acaggcgatg tggatattct agacgataag
     6601 atgaatcaat agaaagaaaa gagaaagtag gttgttggga tgaaattaac ggtaacgcca
     6661 aaagcacaaa aatggtttga ctcagaaatt gatctgccag aaacttacgg aatacgtttt
     6721 tttggaaaag tctacggcaa aacagaggtt catgatggtt tttcgattgg aatgtcagtt
     6781 gaacagccag aacatcctgt aaagaaagtg gaaatggatg ggacactttt ttttatcgag
     6841 gaagcagatg aatggttctt taaaggatat gatttacttg ttgattatga tcccaacctg
     6901 gaggagccaa cctatcactt taaaaaacaa taaaaaaaga gagggtgaca caatttttgt
     6961 caccttctct tttttctttt gaaaatattg ttttcagcta ttaatatcct gcagacaatt
     7021 ttacttgatt ggtttgaagc tcatgggtgg caaagtagct tttcagattg gttaaaaaaa
     7081 tagccattaa tttcttttga aaatcaggtg tcaaacctgc aatgtgggaa gtaatcagta
     7141 cattgtccat tttccaaaga ggatgatctt gtggaagagg ctcttcctca aaaacgtcta
     7201 atgctgcaaa ggaaagttgc tggttattta aagcctgcac cagatcttgt gtcttaacag
     7261 aagcaccacg gccaacattg atgaatacag attttggatc aaagtgttca aacatatgct
     7321 gatcaaagag cccagttgtt tcctccgtta gaggtaagat attgactacg aaatctactt
     7381 caggaagaac ttcctttagt tgatttaaag aaaaagtctt ttccattcct tctgctggat
     7441 gaccggatgt attgattcca tagcagttca tgtctaatgc tttgcatttt tttgcaatca
     7501 tttttccaat atggccagtt ccaatgatta gtattttttt cccttggata gattggtaat
     7561 ggaccgatga acctaaccat ttcatgtcac tctgtgcctt ttgtgcttga aaaagaccac
     7621 gagaataccc aaggatgatt cctaagacat gctccgtaat cgattcactg tggatgccac
     7681 taccatttga aagtaaaata tgtttctttg aaaaagtctc aaggggtagc tgatcaacac
     7741 cagctgagat agattggacc catttcaagg tagaagaggc taatagtttt tcttggtagt
     7801 ctgagttcca gcccaaactg atctctacat tttgtagatc ctctggttgt aattctgttt
     7861 taaagatata accaggcgct atagtatgga tttcttcgat aaattcatca cgaaatggac
     7921 gatctgcata aatgattctt tctgacatag ttaatttccc gcctttcttt actctaagtg
     7981 tagcgcatac aagaagaact ttgaagcgcc ttgcttgttt tttgagaaag aaatattttt
     8041 caaataaaaa aggggctgtg acaaaaaaat gtcacaacct ctaaaagggt ctatttaagt
     8101 agaaataacg agttgtcttt atttcttaat tgatcgatat ttttgcccat cctttgacag
     8161 cttgagcaaa aataaagatt cctgcaaaag caatgaaggc gctagtgtca aaagaaggaa
     8221 gaaagaccat caagattgtt gcaattatag taggcaatga aattgaatag atcaatgttt
     8281 ttaaacaatc aagaaaagta gcttttgtta gtcgaaagcg tgcatagaga aaagcagcaa
     8341 tgttggtaaa tagcaaagtg atcagcagat tcagaaaact aggataaata gaaaatataa
     8401 atgtcaggac ttttatccaa agtggtatag atgcttcact taaaaaagaa cggagctgct
     8461 tacctgttaa gttttttaaa gattcattgg tgtagggaag ttctaattgg ttattgttca
     8521 acaaagcaga agtcgtgcct gtattaggaa gagctatgat aagtttgttt tttaacatcc
     8581 cgacactaac aaaattcccc atcaaatcag tagagatgtc ctgttctgtt cgttttccct
     8641 ctggatcaaa tgtgaagata atggagtttg tttggtaaat gaatccttct ttttcttcgg
     8701 tttgaagctg cccgttttgg attgtaaaat caggaatgcg agtagcaatc ttttgcccat
     8761 ccaattgaat gttatttaag acttgaaaaa cttgaaaaga gattggcaaa gccataatta
     8821 tactcaaaac taaaagatag acaatggctt tccaaaatgg gacatttctc gcctccttta
     8881 ggtctttgaa tcgtataaag gaactgataa ttaattgttt tgttgtcata agtactcctc
     8941 atctagtagt gtcatcactt gcaattctag cgaagtatca agtgaaatac aagtgcttta
     9001 gggaaacatc tgaatgattg gtgaaatatc aaaaaaatgt tattcttgtc taaagactat
     9061 ccaatataaa taaaatggag gaaggaatag tgcctacaca gccacagaga tcatcaaaaa
     9121 aactatttgg agtagtggtt ttgaatgcgg tgatcacttt atcagaattt attggaggga
     9181 ttatgtcagg gagtttgtct cttgtttcag atgctttcca taaccttagt gatacattgg
     9241 caattatttt ttcttacggg gcacaaaaaa tggcccgaaa agaagcaaat aaaaaacgta
     9301 cgtatggcta ccagcgttta gaaatattga gtgctttcat caactcattc atattgatca
     9361 tattatcctt gtttttaacc gttgaagcat tcaagcggtt cacttcacca gaaaagataa
     9421 atagtcattt gatgctaaca gtagcagtta ttggattgct cgcgaatttg ttctcagtat
     9481 tattattacg acaagaagca gatgaaagtt tgaatataaa atctagttat ttgcatttgt
     9541 taagtgatac actgtcttcg gtaagtgtga ttatcggagc aattttgatc cgttttttcg
     9601 atatctactg gatcgatcca gttattacac tgatcatttc gatatatata ttgatagaag
     9661 cgataatggt gatcaaaaaa gctgccgcta ttttgataca aagtgcacct gctattgact
     9721 atgaaaaaat ggaacaagaa attgaagcaa tcgatggggt aaagaatgtt catcatgtcc
     9781 atatttggca gtatagcgaa aagataatta ttttcgacgg acatattgat tttgaagatc
     9841 agctactaag cgagattgaa aagatatatc ctcgtataat ttctttatta aaattaaaat
     9901 atggtattac acatgtgacg atccaagcag agacaaaggc aaaggatcaa aaaaaattaa
     9961 tttttttaaa taaagatgaa agttattgaa atatagagta tgataaataa gaatttttaa
    10021 taaggtaggt tggctcatga agcagtatcg acagttaaaa ggatatttag aagcaaaagg
    10081 atattgggag atactagtct atctcttttt tggtggtttg gctacgattg taaattttgt
    10141 tagttttgct ctcgcacgcc aatattttga tttaagtatg gccttatcca attctatttc
    10201 gtggttttgt tctgtattgt ttgcttttgt aacgaacaaa ctttgggtat tccattcaaa
    10261 atcaccgaat tttacacatg cacttattga atgcggtaag ttcttcttct accgtatcct
    10321 ttcttacgga ttggacatgg gggcaatggt attgttgatc aatgttatga acagcaatga
    10381 gtatgtggcg aaaatcatca ctcaaattat tgttatccta gcgaactata ttttcagcaa
    10441 gttgtttatt tttaaagaaa ctgaggtttt tgaagaagaa gtaggtcaaa gtgaaaaaga
    10501 cgactaggca taacaattga agaatttcct tctctttgtt actctttgta atgtagacat
    10561 tataaagaaa acattgagga ggaatttttt tatgacttac acattaccag atctacctta
    10621 tgcatatgac gcattggaac cttatattga cgaagaaacg atgcatctgc atcatgataa
    10681 gcatcacaat acttatgtga cgaatttaaa tgcagcaatt gagaaatacc cagaattagg
    10741 cgaaaaaaca atagaagaat tattatctga tatggacgct attccaacag atatcaagac
    10801 agctgtacgt aacaatggtg gcggacatgc taaccattca tttttctggg aaatcatggc
    10861 accaaatgct ggtggcgaac ctacaggaga aataaaagaa gcgattaatg aagcttttgg
    10921 tgatttttct tcttttaaag aagaattcaa aaaagcagcc gctggacgat ttggttctgg
    10981 atgggcttgg cttgtaatgg aaaatggaaa attagctatt acctctactg caaatcaaga
    11041 ttctccattg atggaaggaa agacaccaat tctaggtttg gacgtttggg aacatgctta
    11101 ttatttgaaa tataaaaatg tccgaccaga ttatattgca gcattctgga atgttattaa
    11161 ttgggatgaa gtaaacaaac gtttcgaagc agcaaaataa ccatttcctc cgtcatatca
    11221 gtcttaaaaa taatttggaa aataaaagag cgaaagcttg ctagcgtttt ctaaattggg
    11281 tataatgttc ttgcaaggtt acttgcaaga acttttttgg aggctataca tttatggaac
    11341 acggaacggt taaatggttc aacaatgaaa aaggatttgg gtttattaca gtagacggcg
    11401 gagatgacgt atttgttcat ttctcagcaa ttcaaggaga cggcttcaag agcctagaag
    11461 aaggtcaaga agtagaattc acgattgttg aaggtgcacg cggacctcaa gctgctgaag
    11521 tgactaaatt ataataattt gttgaataaa gac
//
LOCUS       DAXWOO010000086        11534 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.086, whole genome shotgun sequence.
ACCESSION   DAXWOO010000086 DAXWOO010000000
VERSION     DAXWOO010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.086"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            341..547
                     /locus_tag="ACGE70_001787"
     CDS             341..547
                     /locus_tag="ACGE70_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFF8693419.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(749..1750)
                     /locus_tag="ACGE70_001788"
     CDS             complement(749..1750)
                     /locus_tag="ACGE70_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFF8693420.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1755..3668)
                     /locus_tag="ACGE70_001789"
     CDS             complement(1755..3668)
                     /locus_tag="ACGE70_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFF8693421.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3836..4342)
                     /locus_tag="ACGE70_001790"
     CDS             complement(3836..4342)
                     /locus_tag="ACGE70_001790"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFF8693422.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4502..4942
                     /locus_tag="ACGE70_001791"
     CDS             4502..4942
                     /locus_tag="ACGE70_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFF8693423.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(4968..5996)
                     /locus_tag="ACGE70_001792"
     CDS             complement(4968..5996)
                     /locus_tag="ACGE70_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693424.1"
                     /translation="MYWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWIS
                     LLRISLQRMKQGHSITYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTE
                     QAYLYFMFGVMNTYLEEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLY
                     VNLAMIHLYSRVFGTKKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNT
                     YYEKNRRLIFQYCMLVRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYS
                     ASADQLPDIVISDYPIDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSI
                     LEKLDFVN"
     gene            complement(6051..6125)
                     /locus_tag="ACGE70_001793"
                     /pseudo
     CDS             complement(6051..6125)
                     /locus_tag="ACGE70_001793"
                     /inference="COORDINATES: protein motif:HMM:NF016901.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6128..6484)
                     /locus_tag="ACGE70_001794"
     CDS             complement(6128..6484)
                     /locus_tag="ACGE70_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693425.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(6782..7756)
                     /gene="sgrA"
                     /locus_tag="ACGE70_001795"
     CDS             complement(6782..7756)
                     /gene="sgrA"
                     /locus_tag="ACGE70_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFF8693426.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSEISTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(7952..8791)
                     /locus_tag="ACGE70_001796"
     CDS             complement(7952..8791)
                     /locus_tag="ACGE70_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFF8693427.1"
                     /translation="MIRIIRLIGSVLIVFVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            8976..9863
                     /locus_tag="ACGE70_001797"
     CDS             8976..9863
                     /locus_tag="ACGE70_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFF8693428.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(10457..11151)
                     /locus_tag="ACGE70_001798"
                     /pseudo
     CDS             complement(10457..11151)
                     /locus_tag="ACGE70_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            complement(11135..11533)
                     /locus_tag="ACGE70_001799"
     CDS             complement(11135..11533)
                     /locus_tag="ACGE70_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFF8693429.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         3437 a         2298 c         1728 g         4071 t
ORIGIN      
        1 cttttggcct cattttaggt tatcatttgt aattttattt cacagttgat atgattgtta
       61 tgtaaaataa aaaagtttag gatacatcga atttttgggg aaattctttt gggggtatcc
      121 taaatctttt ctattatatt cccatatttt ctattattgg tctagacgta tgatggtaaa
      181 ctttgtacct atctgattgc tgaatcatta attgcttatc attagccatc tttgtttcaa
      241 gttcgctaga tgatcatttt attcacatcc taacaaactt gtcacattta tagattctta
      301 atctatttat catacaatta taaaaaagga ggtgctgctt atgaaagcta tccatatttc
      361 tttgcttttt ttcgctagtg cattgatttt atttgctatt catctttgtt cccagcctta
      421 tatcaataac aacggaatat tagtggaacc agcattcttt tgcttaccac tcggcttttt
      481 gtcccttctt gcctccgcag gcttcggatt tgttgctttt tttaaacgat caaaacaaca
      541 aatctgactt ataaaattta cgtaaaagag atcgtgacaa taatcgtgtg atattcccaa
      601 atttcgaata aaaggtgccc cttgccatct ctaactcctc agtcacaagt tacaatattc
      661 ataaaccatg aaaagcttgt ttattgaaat tgctccttgt gacttggagc taagtgacag
      721 attacaacct cttttgcatc ataagacttt actccttgaa aaacattact aacttttgat
      781 atgtttcttg cgcttgttta ttgctgaaat caaactgata ttcatggttc aaaacagcat
      841 ttttctccgc ataatctact tttgtgactt tcacccccgc tttctcaaat acagaagtta
      901 cttcttccat ttgtttcgta aacgtatttg tatttccatc cgttaagaat gtgttttgcg
      961 tccaacgatc cgcatgtctg accaaggcta aacttttgac cttctctgat tgttcaaagt
     1021 ccttattatc aaagtaagcc catccacatt tttcataaag aaagctagag attttatcac
     1081 ttacttgagt aagtgcagaa ggatccaaca gaccagacaa agaaataaac ttcgtaatct
     1141 gcttgttctc caaagtcgga tcaagatgat cagctttttt aatttcttca ttttgaagtg
     1201 ctaacaaata ttctccacta atttgtgcac ctgccgaatt tccaccaatc gaaatattcg
     1261 accaatctgc ttgatctttt atctcttttt tagctgcttg aatggcttcg ttcaattgga
     1321 taactggtgt aggatgtttt gcttccggtg atcgttcata atctatattt acgatgattt
     1381 gctgcgtgtc tgaacataga tattccaaat aatcacgaca atcattttta tcccctccta
     1441 catatgcgcc tccatgcatc cagaaaagaa tcggtaattt tttcttttcc actttcggtg
     1501 tgtagatatc catattatta tttttgtact ttgaaggata ggatatttct ttttttatat
     1561 cgatttgttc gattttttgc tggaaatcat ctggtcgggc atatacttgt tctttttcac
     1621 ttgtgtcaaa ttgttttcta acaagcaagc taatcggctt tggtgtgatt tgattaatca
     1681 aaaatacagt gactaaaata actacgatga taactggtac tgcaatcact attcgcttga
     1741 accatttcat tcatttaccc tctctttctt cgccaaatcc atgtgataat cgtgctaaca
     1801 gataaaaatc cacctgaata aatgagaagc gaatgtttct tttctccagt tttcggcaac
     1861 attctttttt ttgcatctgt ctttttttgt actttcactg tcatctgaat cggttgcgta
     1921 tcatcacttg attgatcctc tcgaggttca ttctttccct tattgtctgc attctctgtc
     1981 ggatcttgct tttttggtgg agctactggg atttttttgt aatagaaaat aatagttggt
     2041 atcgtctcat taaaaatccc ttcagtcggt ccttcaacat ttgctaattc gtatccttcg
     2101 aatgtctttt gctctgtcat ataattttct ccataagctc cgtaaagctg gatttcgtcc
     2161 tggtctttga gtagactttt attgttttca tccacatact ttacgtttat ctgatcatgg
     2221 aacactactt tcaaaaagtc cgttacatgt ttcgtgtagg cagcatggtc aacttcctgt
     2281 gcctttccat gccctgctcc ttctactaat aaaagatctt tataccctcc tgataactca
     2341 tataattttt gcgcgttaga cacaggtact acatcatctg ctgttccatg gataatcaat
     2401 ttaggcaatg aatctgacat tccaatcgta ggtagagcag cttttgtatc caaatgcatc
     2461 tcctgatcaa aatagtattg tttcaaaagg cccataaata aatcttcatg ctcttgatcc
     2521 ccaatgatgt ctaactgatt ttttatggca tccggtagtg cgggggctac tgtctggctg
     2581 tacgtaaaac gtagttgttc atcaattgat gcaaatccgc aattttcaat aattcctttg
     2641 acattgacag gaatatcttt cgctaataca gacatcatcg tagctgctcc catcgaacca
     2701 ccataaagta aaatcttttg agatggccgc tctcgaactt cttgatttat ccagtaaagg
     2761 acgtcatctg actcatattg tccataggtc acataatcgc ctccgctatt accagtcgct
     2821 ctggaatcaa caaataataa attatacccg gccttataaa aatcattgta ttcttctgta
     2881 acgatcccgt tattccaatt ccctcgaaat ccgccatgga tcatgactgt tttatctgaa
     2941 ccttgatcga cataatatgc atatacatcc atcattttgc catcttgttt gtttagtaca
     3001 gtttgatgaa gttgttttgt atttggatag aagcctgtgt tttctgtagg cgttgtccca
     3061 tcatctgtac ctccaagcat cgacatcaac acactgtttt ttgcaaaagc gatatcgtac
     3121 aatagataag tagcaatcag atgtttgata atctctttat ctacaccata atcattaaca
     3181 gcaagctcag tgactaataa ctctattaat tttgacttga tgtcttctgt tggtccatta
     3241 agtatttctg tcatctgttt accgaagtaa ttcaaatatt ctttaccatt gaaattttca
     3301 cctttaccga tatcatcatt ttctgtccca ttctctttgt cacttacttt agcagaagag
     3361 gattcgtcgt ttgacatttt tgtagctgtt gcttcttctg tttgttggtt atctgtggta
     3421 gcggtcgttt cttgatctgt gctagtaaca gatgtctcaa ccgttgtttc agtatttgtt
     3481 tctgtttgtg aagtactcga tttcgtttca tttccagcat cagtagtttc tgtggtttct
     3541 acctcccgag attccgattc ggaagaaatg ctctctccat atactgtcca ttgcgggacg
     3601 cttgtactta cagcaaataa gatgaccaac gctcccttta atacttgttt tgttctcaag
     3661 ctattcactc ctttttatgt ttctaggaga attatatgta aacgcaaaca ttttttatac
     3721 aaaaaataaa tggtttatcc cataaaaata gaacaggatt attttctcat tttacgtaat
     3781 aaagcagaat ggataaacca gtatccattc tgcttttttt aaacgaagaa acaacttatt
     3841 gctccaaaaa tgtcagaaat cgtttatccc aaattttctc gtaaaaagcg attgtttcct
     3901 ttgctttcat aaaaggatta tcataagttg tcgttgctcc tctacacatc ataagctggt
     3961 atcctaatcc atgagcaaac ttcacagtcg tatctataca atactctgct tgtgccccac
     4021 ataattcgat cgaatgacag tccaattcag ttaataaaac actcaaatta gtcttataaa
     4081 atgaattcgc atgggttttt ctcacaaaat aactgtgctc aggaatagat aattccgaca
     4141 atagctccca tggttttgtt tgtggcacta attgagcatc ttcgtgttga acgaaaataa
     4201 ctggtttccc aatcgtttcg tatgttttga ttcttttatt cactaatgct gtcactgttt
     4261 ctaattcgtg tatcggcgaa gaatcaaaac ataccccttt ttgtaaatcg atcactacta
     4321 ttgcgtcggc tgtgtttgac atacttgtgc cttctttctt ttttcttgat ttaatagtat
     4381 ttcttatcta gctagttcca ttatacgtga caacattttc ttattttaca cctctaaatg
     4441 gcacttttcg actatttctt catgttatta tttcataaat acttagctat tggaggaatg
     4501 tatgatgatt aaattgtata caggttctgg ctgctcgtct tcaagacgcg caaaacgttg
     4561 gcttatcgtc catgatatac cgtttatcga acagaactta attcgtcaac cactaaccaa
     4621 aaatgaattt atccacttac taaaattgac aaacaacggc gtgaccgaaa tgctctctac
     4681 tcattctatt agttataaaa atcttgttca cagaatagat gaactcagtt tgagtgaact
     4741 atatattgag attcgtacat ccccttcttt attgaaaaaa ccgattatct acgaccaatc
     4801 tgcaaaactt ttgactggat ttagcgaaga taaaatcagg atgttccttt ctaaaactaa
     4861 aagaaagcaa gagctcaacg aactcttgct ttctggtcat cttgtcgatt ttaaagaaaa
     4921 taatcttttt gcaatgtctt aattttggca taaacaatct gcttttctta attaacgaaa
     4981 tccaattttt ccaatatgga tctatttttc tcattcctga tttttatgat ttcagctttc
     5041 agacgcgccc aatcattttt tgtaggttgt ccattccagt aaaatatctg tgtattcgtt
     5101 tgatcatatg cctttttgtc gataggataa tcgctaatga caatatcagg taactgatca
     5161 gcggatgcac tatattttat tgggcgttca gtcatagata atatcctttt ctttgcccat
     5221 aattcctgtg ttttaccata ctttgattga atatagaata tgacaggttg ttcgtgtttt
     5281 ataaaaatat ccctgactaa catacagtat tgaaaaatca accgtctgtt cttttcataa
     5341 taagtattaa gctctggaat tctgcgagct accccactaa aaaactgttg catgattgga
     5401 tacatcactg gaaatatatg ctgaaaatct ctgtcttctg ctgattttcc aaaagcatcc
     5461 accttctttt ttgtcccaaa aacccttgaa tacaaatgga tcattgccaa attgacatat
     5521 agataatttt tttctgaagt tgtaaaacaa atcttgaagt ttttttcaaa attttctaca
     5581 atagacgatt caatccagcc ccaactttct tgtactttat ctggagtatc caacaaatct
     5641 tcttccaaat aggtgttcat tacaccaaac ataaaataaa ggtaagcttg ctctgttgga
     5701 gtgatgtgat tcatcgacca gaagtttgct tcaaaaaaag gctctagtat gtaatgcgta
     5761 aactcttctt ttgttatcaa tggatgcaca aatttgttca tctcttctgg gacatacgta
     5821 atcgagtggc cttgtttcat tcgttgcaac gaaattctta gtaggctgat ccatttcttt
     5881 ctatctatac tacgcgtata tggcgcatgt ttcgcaaatt tttctaatag ttgacgttta
     5941 ttttttatac ttgataatat atcagaatga ttttcgtcga atgactgcca atacatcaag
     6001 tgaaaaaagt atcggatacg gtattcttct ccttctatcg gattaccttt tttcaagctg
     6061 caagataaat gatagttttt caaaatagta ttaagttctc ttaattttct ttgaatcgta
     6121 cgcaaactta tataattttt tgactaagat tttctaagga ttgataagta cctttgtaaa
     6181 gtgaatccag taaaatataa ccaatcgttc cctctaaaag tatagaataa actttactca
     6241 gtaaaaactg agaagaaaac tcgactttta tcatcttgtt ttctgaaaga aaagataatt
     6301 tatattctgg atacctttcg caaaacgatt ctgcttgttg aatcaccgaa ttcatcttag
     6361 ccagggtgta gtttagatct aaagcgatat ccttcaccac atataatttc tcgtctcttt
     6421 cataacaata gttcaaaaat tgatatactt gcctttcatt gatatcaata aattcttcaa
     6481 acaaagtaca tactcctttt tatctattat ttagacttat tccgacagct acatgaataa
     6541 cgtacaaata gattcgttac tgatttttca gagatactac catgcgcagt gtgcattgtc
     6601 aattattctt gctcttattt tccatttgtg ttcctgttat ttcctgtttc tcaaatatgt
     6661 tagtaaacaa aaatagatct ggcaaagcta caacttcgcc aggcaataca gcactctcga
     6721 atgaaatatt cactcaaaaa tctatcgttt tattcaattt tagatctgtt gcttttattc
     6781 tttatttcac tcgatggaag agagaacgaa aatagcttgc tcctacaatc aaaatgagta
     6841 acacacccat ccatgtaatt agcttgttca aggttctact accagtttct ggtaaatcgc
     6901 tttttctatt atctgtttct ttcgcttttt cttgatgatc aattccacga gaaacaaaat
     6961 caggctgttt tagcacactt tttaggttgc tccctgcatt tctcttttgt tccttagttg
     7021 gtatgatagg taccaaatca agttcattat ttacttttcc tgtgctgctt tcgctgctcg
     7081 tgcttcctgt gctactctca ctactcatgc ttcctgtgct actttcacta ctcgtactct
     7141 ccgtactgct ttcgctgctc gtgcttcctg tgctactttc actactcgta ctctccgtac
     7201 tgctttcgct gctcgtgttt tctgtgctaa tttcactgct cgtactctct gtactgcttt
     7261 caccgctcgt gttttctgta ctgctttcac tacttgtgct atccgtactg cttggcggtc
     7321 caacaactgt ccaactgctt ggtttcaaat tctccgtgat cgtcatctgt ccatcataaa
     7381 aaacaatcaa atcaacttta tctttactct tgatctcatc aatcgatagt tctgcagaaa
     7441 tcattattgt atactcaata ccagcggtcc attgtacgtc ttttaaataa acttccccaa
     7501 taactttttc tgaacttcca ttaccatccc aaccatatgt tagaacagca tgtacatcac
     7561 tagcattcac gcttttgttt ggtttgactg gaatagtaat gacttgttgt gtcatgtcat
     7621 aaacaatccg atcagttttc acagtaaatg aaagattctc atttgcccgt tcatttgcag
     7681 aagtactgat tccaatcatt gctgcaatta tactcagccc aagaataaca aataatacgg
     7741 tcgctgtttt cttcatttct atcactctcc atataattac cttctatttg ataaaggaat
     7801 tatataaaat gaaaatgatg agaacacgac agtcagttgt gaaaatctaa caaaagatgt
     7861 cgtgtattcg cttgaatttg tttacttttt cgtttaatca atgacagact taaaaaagca
     7921 agaccgatat cagccttgct ttcttcatct tttattgata aacagcagtg tatttcttca
     7981 tccgctttgt tttatatgca tagagtaaca aaccaacaat caagactaac aagaataatg
     8041 tagttgatat agatgcttca gccccaaagt ttcccccagt aattattgta ttttgcgatt
     8101 ggatcagagt ggagaaaaaa ccttgttctt gcgttccgct gactggtata cccaaaatag
     8161 ctccttgtgt ccaattccaa gtaatatgag caccaatagc cgtaaataaa cttcctgtac
     8221 tgtagataag tgcgctgaaa aagcaacctg ctaaaaagat attgaaaact gccagaaatg
     8281 tcatgttagg attcaatccg tgcaaaagag caaataatgc agcagatcca atcattgcta
     8341 ttaacggacc aaatctaagc atctgatgct gaaaaaagcc acggaataaa agctcttctg
     8401 atgcccccac tgcaataaat aaaataaaac tcgttatcag cggcacaatg tttgcgtttc
     8461 ctgaatctaa ccgatacgcc cccgtcaaat ataatggact agtaataagc agaaacactc
     8521 caattccaac aacgagacct cccgctaaat acccccagtc ttttcctcga acagaaacac
     8581 cataatcttc tattcttttt ccttcgacct tactagcaag aaataaagct agtaaaaaaa
     8641 cagctaaatg tccgaccgta ccggcaattg ctccaatcca ttcactattt ccaataaaca
     8701 tatccgcaag tgcattattg acaagaattc ctaaaacaaa accaacaaag actattaaaa
     8761 cagatccaat caaccgaata atcctgatca tctttcatcc ctcctttata ttaagtttaa
     8821 caaccctttt ctttcttgtc tcgtgaaaac tatgtaaatg ttctccatca tagagagaat
     8881 agatttttac gaactaagaa tatctacagc aaccttacga aaatgaaaga ctgtctccct
     8941 aattcttctg ttagactgtt tttaataagg aaaggatggg aaattttttg aaaaaacaac
     9001 tactaattat gtgcctttca gcatgtgcaa ttttactatt ctcaggttgt agcaagtcca
     9061 atgaaacgat tgcaacgttt aaaggtggaa cacttacagt tcaggatttt tatgataacc
     9121 caaaagttaa atcaaataat caaacactat taaaaaaaat gattgtcttc aaagcatttg
     9181 atgatgtata cggaaaagag atttcttcaa aggaagtaac aaaagaatat aatgaacaaa
     9241 tcaaaaaact cggtccaaac tacaaggaac aattaaaagc agttggccaa acagaagaga
     9301 catataagaa actctttaaa cagatgctgg ctttccaata cggtttgaaa gccaatgtaa
     9361 aattaacaga taaagatcta gacactgctt ggaaagaatt ctatccagaa gttagtacac
     9421 agatcattct attttcgact aaagaagaag cagataaagc aaaaaaagaa gcaaatgaag
     9481 gagaaaattt ctccaagctt gttcaagctt atggaaaaaa taaaacatta aaggaaactg
     9541 atggaaaaat gaattttgat tcaaccaatc cagagattcc tactgaagta aaaaaagcag
     9601 catttaaact aaaaaacggg gaagtttctg atatcattcc tgtaacagat ccaacgactt
     9661 atcagcaaag ctattatcta gtaaaaatgg taaaaaaaca ggataaaggt tctaataaag
     9721 acaaatacaa atctgagcta gaaaaaattg ctaccgaagc aaaaactctt gatacggaat
     9781 tcatggataa aacaatccgt aaagtgatga aaaaagacaa tgtaactatt aaagatcctt
     9841 atgtaaaaaa tatattcaaa taaacaaatt tgacccaatt ggggatgtgg caaaatttga
     9901 gaagtgattt ttggaaattc cttcttattt ttgaggcaaa ccacttttgc cacaacttca
     9961 tcggggcctt tttttatttg taccaaaaca aatcactgga tttgttacaa tcatgaggat
    10021 ataatttaaa aaagtttttt atttctatca catgaatcaa tgcgccatta agtagattct
    10081 ttcatgcttc cctatttttt cacttgccgt atcgcttcaa aaacaactat cataaactca
    10141 aactcgactg aaaagttatt ttaaaaaaga ctaaaaatat cccttctgga cttgttcttc
    10201 ttattctgtt atactagaga aacacgaaag atatttttat gcgacacttt tcatttgttt
    10261 ttatgaaatt tattcaaaac tggcgtaact tcagagttac aaggatgtat tagaggttag
    10321 ggaatacatc gtttaaatga gaaataagta atagttagta atagtcaccc aaactttaaa
    10381 ttaatgaata ttgaatcgtc agatagcctc ctcgagtttt cgagaaggct atcgtttatt
    10441 ttatggagaa attgattcag aagtaatcag cgaccttttt tagttcacca atcgtttctg
    10501 cttggaaagt ttgcacccaa tcttcttgga agcccctcac tgctttatca atcgagggca
    10561 tctctaatac ttgatctagt agctctttac ctaacgttat cgctgagctc cagatcgaat
    10621 ggctgtagtg acttgttcac aatttttgaa actagctgct aatatttttg ttggtctttg
    10681 cgtacgttcg atctcagtcc gcaacgaatc aatcactttc atagcatcaa tgccttgatt
    10741 ttgcatcctg ttataataag gtgcaatata atctgctcca ttattaatag ctaaataccc
    10801 ttgtaattct gtgtaaatag ctgtaccggt aatcaaataa ccttcttttt tcaacttttt
    10861 gatcgcttga aggcctgctt gattcaccgg caccttgata aacgtatctt ttcccagttc
    10921 ttctatgata actcgagcat cctctatcat ttcttcttcc gttcttccta ttgtctgaat
    10981 atggatagga atctgtccga ttattttttt gatttctttc atatgcgaga agaagtttga
    11041 cgctccttct tttttcagaa ttgtaggatt tgttgttacg cctgttaaat tcaaaaactg
    11101 ttgacaagaa taaatacttt ttgtatttgc tgtatcaagc atgatttcca tatagctttc
    11161 ccccattctg atcctcttga tcgattttaa tgattgtccc ctttcttaaa tgcggcattt
    11221 ccttatcttc tacatacatt gttcctggaa gttccggctc atgagatcca tcaaatttta
    11281 tcgtgatatg tcctaattca ttcaaattag tttggacaac atctcctact gctgtgatta
    11341 catacatttg tttatcaaaa cagagcttca tatcacaagt tatttctttt cttacctgat
    11401 tcaattcaat cttataacaa taatctgtta atgtttccgg tgcttcatct ccaaataaaa
    11461 tcattatttt ttctttcctg aatacttctg cttctatacc aacatctgtt acaaccgttt
    11521 caaagatcgt catc
//
LOCUS       DAXWOO010000087        11394 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.087, whole genome shotgun sequence.
ACCESSION   DAXWOO010000087 DAXWOO010000000
VERSION     DAXWOO010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.087"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(128..370)
                     /gene="acpP"
                     /locus_tag="ACGE70_001800"
     CDS             complement(128..370)
                     /gene="acpP"
                     /locus_tag="ACGE70_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFF8693430.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            complement(492..1493)
                     /gene="plsX"
                     /locus_tag="ACGE70_001801"
     CDS             complement(492..1493)
                     /gene="plsX"
                     /locus_tag="ACGE70_001801"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFF8693431.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            complement(1542..3578)
                     /gene="recG"
                     /locus_tag="ACGE70_001802"
     CDS             complement(1542..3578)
                     /gene="recG"
                     /locus_tag="ACGE70_001802"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFF8693432.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALTGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            complement(3695..5377)
                     /locus_tag="ACGE70_001803"
     CDS             complement(3695..5377)
                     /locus_tag="ACGE70_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFF8693433.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            complement(5429..5791)
                     /locus_tag="ACGE70_001804"
     CDS             complement(5429..5791)
                     /locus_tag="ACGE70_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFF8693434.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            6061..6249
                     /gene="rpmB"
                     /locus_tag="ACGE70_001805"
     CDS             6061..6249
                     /gene="rpmB"
                     /locus_tag="ACGE70_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFF8693435.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            6487..9156
                     /locus_tag="ACGE70_001806"
     CDS             6487..9156
                     /locus_tag="ACGE70_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFF8693436.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(9247..9861)
                     /locus_tag="ACGE70_001807"
     CDS             complement(9247..9861)
                     /locus_tag="ACGE70_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFF8693437.1"
                     /translation="MINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKAMH
                     MVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPGIE
                     KRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTIIK
                     ITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            9979..10380
                     /locus_tag="ACGE70_001808"
     CDS             9979..10380
                     /locus_tag="ACGE70_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693438.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANS"
     gene            10515..10853
                     /locus_tag="ACGE70_001809"
     CDS             10515..10853
                     /locus_tag="ACGE70_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HFF8693439.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
     gene            complement(11234..>11394)
                     /locus_tag="ACGE70_001810"
     CDS             complement(11234..>11394)
                     /locus_tag="ACGE70_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HFF8693440.1"
                     /translation="IKNHEKFLIRNYKISGGGCYLLECRFTSKKSMNSFLEQLNEYAN
                     YKLSIVID"
BASE COUNT         3264 a         2291 c         2018 g         3821 t
ORIGIN      
        1 tttgcaacat aatttctgtt tatcgccaga attatcagaa aaatgacttt tacgtgtcaa
       61 aaagattaaa aaaagaagga taaaaagctg tatgcagctt tttatccttc tttgctttta
      121 tttgaaatta tttaagatgg ctgctgatat aatctactgc gccaccgact gtttcaattt
      181 gttcagcgtc ttcatctgaa atttccgtac cgaattcatc ttcaagctct aaaacgaatt
      241 ccataatact aattgaatca gcttttaaat catctttgat gttcaattca tccgtcactt
      301 tatcggtatc tatctcaaaa tgatttgaaa taattttcgc tactttgtta aatacttctt
      361 cgcgagtcaa ctttttcacc tccaagaatc atacacttgt caattaccta caccatggct
      421 aattttactg gcatttttca ggaatgtcca cctttttttc aatggaacgg attgcttcag
      481 atatccaaat actattcttc gcttttttca aattgttcga ctaattgtcc aactacatct
      541 gtctcaagca ttgtgtggat ttggcggatc gtatagcgaa cagcatcggg gcctgttgct
      601 ccatgagttt taatgactgg tgctttcaag ccaaacaaaa cggcaccgcc atgtttggaa
      661 taatccattt ctgctttcaa agaacgtaag ccgtctttta aaagcatagc gcccattttt
      721 cctttgatcc cttcatttag aatagcagat tttaatagat tcatcatatt cattgctgtt
      781 ccttcaatag atttcaaaac agcgttgcct gtaaatccat ctgtgaccac tacatcagcc
      841 acaccattga gtagctcacg agcttctacg tttccaataa aattcaagct tgtttcattc
      901 tgaaggagtt cataggcttt tttcgtcact tcactacctt ttgttgcttc tgttccgttg
      961 tttaagagtg cgacacgagg acgtccgata cctcggactt tacgtgcata aaaagaacct
     1021 aaaataccgt attgcaacaa atgttctggt ttgttttctg cgttagcgcc aagatccagc
     1081 atatcgaaac cggcaccttc tttccctact actggcaagg tagacatcaa gccagggcgt
     1141 tcgatatttt tgatacgtcc tacgatgaac aaccctgctg ctagtagcgc gcctgtattc
     1201 cctgctgaaa atacagcatc tgcttcaccg tttttcactg cttgtgcagc aaggaccatt
     1261 gatgcagtct tttttcgtcg aattgctttt actggctcat catcactatt gattttttcg
     1321 tctgtatgga taatcgtgat gtttttttca tttgttaagt atttcttgat ttctgcttct
     1381 tttccgtaaa gtaagaattc tacttctgga aaatcttgct gcgctaaagc cacgccttct
     1441 acaattgctt gaggtgcgtg atctccgccc attgcatcta cggcaatttt cattgtttcc
     1501 actccttttt ccgtcatcag tataaagtat agcacagtct gctaatcgaa ataacgatta
     1561 tcctgatttt ctatttggag gctttgcgct aaaggctgat actcaggaag catctgccaa
     1621 tcctttatct gccaaagggc tgcagcctct tctctggcta cctccaaaac atttccatcc
     1681 gtcaccaagt cagcaatcgc aaattgaggc agaccagatt gacggaagcc aaatacttca
     1741 ccaggaccac gtaattccag atctttttcg cttagtacaa aaccattggt ggtctctgtc
     1801 atgattttca tccgttccac acctagttca tttttaggat tagcaactaa gatacagtag
     1861 gaagcatcac ttccgcgtcc aacacgtcca cgaagttgat gcagctgtgc caatccaaat
     1921 cgatccgcat ccatgatcag catgacggtc gcgttcggca cgtttacccc tacttcaatt
     1981 acagtagtcg aaactaatat ctgcatctgg ttgtctttga aggcttccat gattacttct
     2041 ttttcctgat tcttcatttt accatgtaac aagccaactt catattttgg tgcaaatagt
     2101 tcacgaagtt tttcatagat ttccactgca ttttttacat ctaatgcttc tgattcttca
     2161 atcagcggac agatgacata catttgatgt cctcttgcca gttctttttt cgtccaatcc
     2221 agtacactgt ccaattgagg tgttcttacc cagcgtgttt cgatcgggat acgtccagct
     2281 ggaagttcat caatgataga tacatccatc tcaccatatg ctgtaatagc taatgttcta
     2341 ggaatcggag tcgctgtcat aaataagaca tctggtcgcc agcctttttc acgaagtact
     2401 ttccgctggt tcacgccaaa acggtgctgc tcatccgtaa taactagacc taaattctga
     2461 aattcgactc catcttggat caatgcatgt gtgccgatca cgatgttaat ttccccttgc
     2521 tctagttttt caaggagctc acgacgttct tttgctttcg ttgaccccgt aagcaatgcg
     2581 atggttactt cattgggatc aaacagctgt tgcaagcttt ccatatgttg ttgtgccaag
     2641 atttctgtag gcaccatcaa cgcaccttgg aaacctgctg taacggtagc gtataacgca
     2701 atcgcggcaa ccactgtttt tccgctacct acatctccct gcaaaagtcg ctgcatatgc
     2761 tgcggactgc gaagatcccg acagatttca ttcgttacac gtttttgtgc atttgtcagt
     2821 tcaaaaggta gcttttgagt aaattctttc aatttttgga catcataaag gatctccaaa
     2881 ccattcgttt ctgctttttc tgcttttttc aatccttgaa gacgcatttg aaataaaaag
     2941 aactcttcaa aaacgacacg tcgtttggct tgatggtttt cttcgtggtc ttttggaaaa
     3001 tgcatacttc ttacagcaga agctcggtca agcagtcgat atttctccac aaggttgttt
     3061 ggcagatttt cttcaatcaa tgtgccgtat tcttgaaacc cttttttgat tagatcaact
     3121 aatgtcgttt gacgaattga tttgttgacg tgatatatcg gcgaaaagtc atccattgtt
     3181 tgagaaccta aaatcttcat cccggtcaat gctttacgct ttgcatccca tttaccgtat
     3241 acggcgattt cctccgataa aatgactttt tcttttagat aaggttgatt aaaaaaagaa
     3301 acgttgaaca catcatgttc ttgcatcata cgaaattgca atcggctttt tttataacca
     3361 aatcgattca tcacaggagg cgagactacg attcctttca aagtaacctt ttcttgatct
     3421 tggatctcgt ttagatttct ttcctgaata tcttcgtagc ggaaaggata ataagtcagt
     3481 aagtcttcaa tcgtttggat gcccaagctg gcaagactag ctgctctttt ttctccgact
     3541 cctgccagca tagtgattgg atcatctaaa gaacgcataa atttttcctc cctcccttaa
     3601 caaaaaaagg acttggtcag cgatcatacc tgatcactaa ccgaaggcct tttatgaatg
     3661 aatactgtgt tagaaagata ttctgacaca gaaattattc agcagaaaat agataaggat
     3721 agacaggctg atctccttgg tggatctcga tttccaaatc aggatgtttt tctgttagtt
     3781 tctcagcaaa ttcatttgct tgttcaactg ttccttcatc acctgccaag atcgttacga
     3841 tctctgtgtc ctcattgatc atttgttcta gtgtacttaa cgctgttgct gtgatatctg
     3901 ggttagagat caggattttt ccatcgatca tgcccaaaaa gtctccttct ttgatctcta
     3961 caccgtcaat tgaggtatct ctgatcgcgt gagtgacttg accgctcaca acgctagaca
     4021 acatttctgt catcgtttct ttgttttctt ctaaagaagc ttgatcattg aatgaaagca
     4081 tagctgtcaa tccttgggaa atcgttcttg aaggaacaac tgctactggg atatctgcta
     4141 cttctgctgc ttgatcagct gccatgaaga tgttcttatt gtttggcaag acaatcactt
     4201 tatctgcgtt tacttcttga atcgctttaa caatgtcttc cgtacttgga ttcattgttt
     4261 gcccaccact gataacataa ttagcgccta aactttcaaa cagctcttgg acgcctttac
     4321 cagcagcgat cgctacgata ccatacggga tacgtggtgt ttcttttttc gcaaactcgt
     4381 tcacttcttc atcgtgttcc aaaatcgtct catgctgcaa gcgcatgttg tcgactttga
     4441 ttttgactaa tgaaccgaat ttttgtccat aattcaagac ttctcctggg ttttctgtat
     4501 gcacgtggac tttgatgatc tcgtcatcat tgaccacaag taaagaatct cctataccat
     4561 ctaaatagtt acggaattct tcgtaatcga atgtactatc gactgtaggt ccttctccga
     4621 tttttaccat gatttctgta cagtaaccaa aatggatatc ttctgtcgcc atttgacctt
     4681 ggacactgcg atgatgttct gcattgacca tatcatccat ttctgctgga gaaggttcat
     4741 atgtttcatc cgcttggaat tctccattca atgcacttaa aaagccctca taaacaaaca
     4801 gcagtccttg ccctccactg tctacgacac cgacttcttt taggactgga agcaagtctg
     4861 gagttttagc taaagcacgc tttgcccctt ttacaacagc tgtcattact tcaacgacat
     4921 catcagtact ttttgctttc ttttctccag ctttcgctgc ttctctagct actgtcaaaa
     4981 tcgttccttc tactggtttc attactgctt tatacgcagt ttcaactcca tgagtgaagg
     5041 ctgcagcaag atcggaagca tttaatgaga caacatctgg aatttgctta gaaaatccac
     5101 gaaacaattg agacaaaata acgccggaat ttccgcgcgc acccatcagc aatccttttg
     5161 aaagcattgc tgctaattct cctacttttt ctgaggttga atctactact gccttagctc
     5221 cactagtcat agatagattc atatttgttc ccgtatcgcc atctggcaca gggaatacat
     5281 ttaatgaatt gacgtattct gcatttactt gcagacgatt cgcacctgcc tggaccattt
     5341 cctggaactg gcttgcactt atttctgtta cactcaccta gataatcctc cttcagatcg
     5401 actgctgagt taccagccag tctcctgcct agtctggcag tacacgaaca ccttgtacaa
     5461 agacattaac tgaattggca gttacgccta ataatgcctc tagattgtat ttcacttttt
     5521 cttggacgtt gcgggaaact tcagagattt tcgtaccata gctgataatc gtataaacat
     5581 ctacagcaat accattatct tcttggcgga caactactcc tcttgagtaa ttttctttac
     5641 gcaaaatctc agtaatatta tctttaattt gatttttgct ggccattcct acaacaccga
     5701 acacatctgt cgcggcgcca ccaacaactg ttgcaattac atcattggta atttcaatgg
     5761 ttccagctga cgtttgaatt tttacagcca tgaatgagcc tccttaacgg gtaaaaaact
     5821 cccatttttc ttctatcata ttttatcata gcgcaacttg aaataaaagg cagttaccca
     5881 aagtactcaa aaaccttgca aaatttcagg tataaccttg tttaatttac aattattctt
     5941 ctcaattgtc aattgtatct atgaaaatgt acttgcaaat ttccattcta tatgatatgt
     6001 tagtctggta tgagctgaat tattctattg ctccaaatca aggcaaagga ggaaattacc
     6061 atggcaaaag tttgttactt tacaggtcgc aagacaaaaa gtggaaacaa ccgttcacac
     6121 gctatgaact caacaaaacg tactgttaaa cctaacttac aaaaagttcg cgttatggtc
     6181 gacggaaaac ctaaaaaagt ttgggtgtca actcgtgctt tgaaatcagg aaaagttgaa
     6241 cgcgtttaat tgaatgatac ttggaccgtt cagatagtga acggtttttt atttaataaa
     6301 aatttttaag ggtgggactt ttgattccta cccttttttt gcgtttattt ttgttatacg
     6361 aacaaattat gaagttattt attctaaatt ttattttttt gtgaaaatta gactcttttt
     6421 aagcagagaa aaacaaggat atgataaact gaatacaact tcatagaaat ttggtggaaa
     6481 ataaatatga aaaaaaattt aaagtcattc tttagagaaa acggacgcta cgcagcagct
     6541 agtttcctga ttccgttttt gattttagca cttgtttatc taagtatcgg gatctatcct
     6601 ggaagcagca gaagtatttt ggcaagtgat gccttttcac agttttctaa ttttcatgcc
     6661 agcttccgaa atgttcttct tggaaagcaa agtatttttt atacatggaa tgcttctcta
     6721 ggattgaatt atttatcttt aatttcttac tatctaggcg ggatcttcac accactggtg
     6781 ttcttttttc caaaccagat gatgccagat gcactgtatg ttctgacttt gcttaaggtc
     6841 ggctgtgccg gattggcttt ttggtttctt gcacgtacgt tcaagattcc tcgctggggg
     6901 catgttgcac ttagcgtcag ttacgcattg atctcattta tcactgcaca ttcggagatc
     6961 atcatgtggc tggatgcatt tgtttatctt ccattagtga ttttggggat tcatcgtgtt
     7021 atggatctta aacggccgaa acttttattt atcagttatt tactcttgtt tttatccagt
     7081 ttttatatgg gttttatgat tggcgtattt tctttcttat attttatcgc ccgtctattg
     7141 acaaactggt cagaatataa aaaaacgatt gttccttatg gtatcacttc tttactggca
     7201 ggcggcgctt caatgatcat tgtattgcca gcagtgctgg atcttcggtc aaatggcgag
     7261 gcactgagtc agatcactac gttgaaaaca gaagcgactt cttatctgga tctaatcatg
     7321 aaaaatatga ttggtgttta tgacacgaca aaatacggat cgattccgtt cgtttacgca
     7381 ggtctgctac cattagtcct ctgtctgttt tattttgcca gtcggaaagt accgttgaaa
     7441 aataaatttt tgtacggcag tcttttcgcc atacttatcg caagtttcta tcttgttcca
     7501 ttgaatttgt tctggcatgg tatgcatgca ccgaatatgt ttttattccg ttatgcctat
     7561 ctgttctctt tcttagtgct cctgcttgca ggaaaaggat gggaaaagat agaacaagac
     7621 gatcgtgggc tgttgttagg aatcattttg gtcgtagctg ctctctttgc ggtggcatgg
     7681 ggcatcaaac ctgaaggaag ctacacctat gtgacgatga cttcctttgt attgacagtg
     7741 gttttcttag ggatttatgc ttttgctgtg ggattttatc agcataaaaa actttcattc
     7801 aatcatctga gtatattgtt attattgatc atgtctgctg aagcactggt caatacgaac
     7861 agtatgatcc atggcattct tgatgattgg aattatgctt cccgcagttt atatactgat
     7921 ccttatcctg acttgaaagc tcttgtaaat gaaacaaaaa aagattccga ctcgttttac
     7981 cgcttggaaa atctggatcc agtatctgca aacgattcga tcaactatgg ttacagtggt
     8041 atcagtttgt tttcttctat aagaaatcgt aattcttcct cttacctgga taaattaggt
     8101 ttccgttcaa gagggacaaa tttgaatatc cgttataaca ataatacttt gctaatggat
     8161 gggtttacag gaatcaaata taacatttct aaaaacgaaa gcacgttcag taaatatggc
     8221 ttccgccaag ctggtcaaag tggcgactat acactgtatc agaatgtcaa tgcattgcca
     8281 ctagcattta ctgcaccgtt gtccgttaat gatgttgagc agccagtgac agataatttg
     8341 accagtcaga caaacttgat caatacattg tctggtttaa atgaacagta ttttacattc
     8401 tatacaccta ctttgaagaa tcagttgaat acgacgatca caaagacaac gactggcgtc
     8461 acttactcag aaacagcaga taaccagcca aaagagctca cttgggaagt ccatgtgcct
     8521 gcaaatacac aagcttactt aagtttgttc ccaaccaatt ttgcacaatt agaaagttct
     8581 agtgcgacga tcactgtcaa tggtacatcg cgaaaaacgc aaatcaacat ttctggtcaa
     8641 tactatgatc ttggctatta tcctcaagag acaacagtga catttactgc aagtttttat
     8701 ggaacgaaag aagtaagttt catggagcct aaagtcgttg gtctagatgt tgtagcctat
     8761 caagccgcaa tgaatcagat caaagaaaaa ggtgtcgaaa tggaagccac tggcagaaaa
     8821 gcttctggga ctgtaaatac cgctgaagat tccatgttgg tgacaactat tccttatgac
     8881 aaaggatgga aagctaaaat tgatgggaaa ccagtcaagg tcagcaattt caaagacgca
     8941 ttcttgatga taaaagttcc agcaggtaaa catactgtca ctttcagtta cttgcctgaa
     9001 ggatttacga taggtgcaat cctttttgtc ctatgtattg cattgtttat tctttatgtc
     9061 tggtaccttg accgcaatcg ccccttttat ggcacgattg atccagcaat agaagaaaaa
     9121 ccgcgaaata cacacagaag aagacgaaga aaataaagaa acgatggaat ctgtgtcact
     9181 cttctattta aatggcaaaa atttcaatct ttaaatgaca aaaaccacct gatttttggt
     9241 ggttttttat atacctatga ctaaactatc tcttgctgct tcgacaactt ctacagtgat
     9301 cttatccatt tgattgattc ttaaaatccg atcaatctgc gcaaataaac gatgaataag
     9361 cctaaaattt ccttttgtga tcttaatgat agttgtgata gcctcataat cagaaaaatc
     9421 ttctagcttg atatcaattc ctaaatctgc ccatttgtat tccaaaatat gatgtgtttc
     9481 gtccttacta aggttatcaa attcatgtgc aaatcctatg cgagagtaaa gctgcggata
     9541 acgagacaat cttttttcaa taccaggcat accgataaga accattgcca gatcatttct
     9601 atcgtaaata tctcttagct gttctaaatg ctgaagcttt aaacgatcaa tttcatcgac
     9661 aataattaag tcaattgctt cataggtttt aggcacagga ggatcttctt gattgtattt
     9721 tagtaccata tgcattgctt tagtcagacc tattctacta gttattgtgt taatatccga
     9781 actgacacga gacatctttt cagctggtac agtataaaaa actttattta cacttaatat
     9841 ttcatctgtt gcatttatca agaacaagaa aaagtctgaa tattcaaaaa ataacggtgt
     9901 acatttttaa aaggagatgt ataatcgaga tttagaaagc gttttcctaa aaatttaatg
     9961 aattaggaag tgattactat gaaaaaatta tttagcgcac tttttattct gttatttggc
    10021 tttattggta ttgggacttc tggaaatata gttaaagcta gcacgatggt tgaacaaaca
    10081 agtgaaatgg caaaaacttt tagtcaatta acaccagaag aacaaagtta cttcaaatcc
    10141 aaaggattta gtgataaaga tactttttac actcaaaaga tagagcctca agatccgaaa
    10201 acacgttttt ctgcaaatgt attgcggtta accgcatcta ctaaaaaaat tactaatacg
    10261 caggggcgga cagaatatat tataacaagt cccaattcgg cgatgtatcg tatttctaca
    10321 cgcttgagtt taggtagtgc aaaatcatat actcaaacag taaatctagc taattcttaa
    10381 ggttatggtg gaggaatgta ttttacattt actgggaaaa gacaatatta cagtgtaaaa
    10441 ttagctatgc aagtttataa tacatttggt gtagggtcag tctcttgcac tgcgggagga
    10501 ttgacaatag gaaaatgata atttcttact atgtcttaaa tagaagtgat gatgaaggac
    10561 tcttcgtttt tcaatcatta catgaggata cgatgctaaa agagttttat aatcggatta
    10621 agaataaaca gatagaaaca aatttatatt ttttaagtga ctctatcttg ccttttatta
    10681 gcaaccctaa aattcttaac ttgttgcaga caaaagattt gaaaagttta ccgattattg
    10741 tcattgatga ggaaattgta aaatatggcg cttatctaag cgtagaagaa ttagaaaaat
    10801 taactaacat tggaatgagt attcaggagg ataatggggt acatggaaca tagaatgaat
    10861 atgcacagtt gagaacggaa tttgacattt atttagacca acagggtata ccctgttggt
    10921 ctttttgtgc gttcatgata tacttggtgt catttttata tttaaatggc acaaatattt
    10981 ctaattattt gtcatttaac gctgaaaatt tttgctattt atctaaaaaa gtgacaggat
    11041 actcatttgg ccttcataga attttctaaa tatgaatcat caatgaattt aattaaaaaa
    11101 ataagtaggg caaaatataa atgtttttgc cttacttgtt tccatcagtt tagttttaaa
    11161 ttgataagtt atatagttat ggttgaatgt gtgtctcgca tattagtatt tttaccatct
    11221 gctagtttta ttattaatct ataacaatag ataatttata attcgcatat tcattaagct
    11281 gttccaaaaa gctgttcatt gatttttttg aagtaaatct acattccaaa agataacagc
    11341 cccctccact aatcttataa tttctaatca agaatttttc atgattttta ataa
//
LOCUS       DAXWOO010000088        11253 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.088, whole genome shotgun sequence.
ACCESSION   DAXWOO010000088 DAXWOO010000000
VERSION     DAXWOO010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.088"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="ACGE70_001811"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="ACGE70_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFF8693441.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="ACGE70_001812"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="ACGE70_001812"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFF8693442.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="ACGE70_001813"
     CDS             complement(2169..2906)
                     /locus_tag="ACGE70_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFF8693443.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="ACGE70_001814"
     CDS             complement(2997..3524)
                     /locus_tag="ACGE70_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693444.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="ACGE70_001815"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="ACGE70_001815"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFF8693445.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="ACGE70_001816"
     CDS             complement(4401..4886)
                     /locus_tag="ACGE70_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFF8693446.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="ACGE70_001817"
     CDS             complement(5039..5236)
                     /locus_tag="ACGE70_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693447.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="ACGE70_001818"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="ACGE70_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFF8693448.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="ACGE70_001819"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="ACGE70_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFF8693449.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9211..9423
                     /locus_tag="ACGE70_001820"
     CDS             9211..9423
                     /locus_tag="ACGE70_001820"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFF8693450.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9425..11104
                     /locus_tag="ACGE70_001821"
     CDS             9425..11104
                     /locus_tag="ACGE70_001821"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFF8693451.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
BASE COUNT         3265 a         2429 c         1971 g         3588 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata
     9001 acaattaatt gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga
     9061 taagcaaaaa cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga
     9121 aaaaccaggt ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca
     9181 ctgctttacg cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca
     9241 aaagtacgca accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat
     9301 gtagacgctc gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt
     9361 ctagaaggaa gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt
     9421 taatatgaaa ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg
     9481 tgaaatcggg aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc
     9541 cgggatcaaa ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag
     9601 ctacatcata caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga
     9661 ccacatcggc ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc
     9721 tctcgcatta gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga
     9781 gctccatgag atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt
     9841 cagaacgacc catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa
     9901 tatcgtagcg actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt
     9961 gcataaaatg gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa
    10021 tgctgagatt ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat
    10081 cttttcaaaa atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt
    10141 gcagcaagca gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag
    10201 catggaaaat gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac
    10261 atttgtagat gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac
    10321 aggttctcaa ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca
    10381 aatccaaatc cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac
    10441 aacaagtgtc aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg
    10501 aaaaatcaat aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct
    10561 tcgtttgatg aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt
    10621 acatgcttct ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa
    10681 tggcgatgtt ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg
    10741 agatgtgtat atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg
    10801 tcacctgctt tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa
    10861 agaaatcttg gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg
    10921 agaaatgatc aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg
    10981 acctgactgt tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt
    11041 attcgaacgt acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga
    11101 ataaataaga ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa
    11161 acatgagaag ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc
    11221 tcttaaattt atttactttt tcatacaaaa aaa
//
LOCUS       DAXWOO010000089        11084 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.089, whole genome shotgun sequence.
ACCESSION   DAXWOO010000089 DAXWOO010000000
VERSION     DAXWOO010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.089"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(6..2015)
                     /gene="metG"
                     /locus_tag="ACGE70_001822"
     CDS             complement(6..2015)
                     /gene="metG"
                     /locus_tag="ACGE70_001822"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFF8693452.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            2283..2561
                     /locus_tag="ACGE70_001823"
     CDS             2283..2561
                     /locus_tag="ACGE70_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693453.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            2658..3479
                     /locus_tag="ACGE70_001824"
     CDS             2658..3479
                     /locus_tag="ACGE70_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFF8693454.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(3764..4774)
                     /locus_tag="ACGE70_001825"
     CDS             complement(3764..4774)
                     /locus_tag="ACGE70_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFF8693455.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(4786..5739)
                     /locus_tag="ACGE70_001826"
     CDS             complement(4786..5739)
                     /locus_tag="ACGE70_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8693456.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            complement(5729..6799)
                     /locus_tag="ACGE70_001827"
     CDS             complement(5729..6799)
                     /locus_tag="ACGE70_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8693457.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            complement(6805..7848)
                     /locus_tag="ACGE70_001828"
     CDS             complement(6805..7848)
                     /locus_tag="ACGE70_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8693458.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            complement(7849..8790)
                     /gene="opp3b"
                     /locus_tag="ACGE70_001829"
     CDS             complement(7849..8790)
                     /gene="opp3b"
                     /locus_tag="ACGE70_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFF8693459.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            8874..9221
                     /locus_tag="ACGE70_001830"
     CDS             8874..9221
                     /locus_tag="ACGE70_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFF8693460.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            complement(9291..10952)
                     /locus_tag="ACGE70_001831"
     CDS             complement(9291..10952)
                     /locus_tag="ACGE70_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFF8693461.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
BASE COUNT         3111 a         2261 c         2023 g         3689 t
ORIGIN      
        1 aatttctatg cgatttctga gccatttggc atggattttg gtgcttcgat tacttgtaaa
       61 gagccatctg gtgcttctgc agaaaggatc attccttgac tgatttgtcc acgcatcttt
      121 cgtggtttca gatttgctac aatgacgact tttttcccga tcaattcgct tggatcagga
      181 taaaattcag cgattcctga caagatttga cggtcttggc tgtctccagc atctagtcgg
      241 aattgcagca acttatctgc cccttctact ttttgacagt tgatgacttc tgctactttt
      301 agttctactt tttcaaatac atcaaatttg atttgttttt ctttcgttga aacaagttct
      361 gtttcttctg gatcccattt gactgtatct tcgtttgttt gagtaccttc agacattttt
      421 tcttgaataa aagcaacttc ttcttccata tctaaacgtg ggaagattgg tgtacctttt
      481 gcaactactt ttgttcctga aggaaattcg ccaaagtgta atccttcaag attcattgtt
      541 tcagagtcca atcctagttg attgaagatc ttctctggcg tttctgtcat gattggctga
      601 agtaatatcg ctgcgatacg caaagattca gctaaatgga tcatgacact attcaattca
      661 gctttctttt cttcgtcttt tgctaatacc catggttctg tttcatcaat gtatttgttt
      721 gcacgagaaa tcagcaccca gatttcagcc aatgctgtat tgaattccat tttttccatt
      781 gcatcatggt aacgtccgac aacgttagca gccgtcgtag acagttcact atcaaatgga
      841 gtgacaagtg aggcatagtc aggaacgagt ccatcacaat atttgttgat catagcaatc
      901 gttcgattca ataagttccc tagatcattt gccagatcat aattgacacg ggaaacaaaa
      961 tcttctggcg taaagacacc atctgaccca aacggaacag cacgaagcaa ataataacgg
     1021 agtgcatcta aaccataacg ttccacaagc atttcaggat aaacgacatt tccttttgat
     1081 ttagacattt ttccgtcttt catcaatagc cagccatgcc caaatacttt tttaggtaga
     1141 ggcaaatcaa gggccatcag catgattggc caatagatcg tatgaaagcg aacgatttct
     1201 ttaccgacca tctgaacatc tgcaggccag tatttttcga atagactttc atcttcggag
     1261 ccatatccta gtgcagtaat ataattggat aatgcatcga tccatacata caccacatgt
     1321 ttcggatcac tcttgactgg tactccccaa gtaaaagttg ttcgtgaaac tgcaagatcc
     1381 tctaatcctg gtttgataaa gttgttgatc atttcgtttt tacgagattc tggctggatg
     1441 aattcgggat gttcttcata gtattccaat agacggtctg cgtatttgct catgcggaag
     1501 aagtatgatt cttcttttac caattctact tcgtgtccac ttggcgcttt accaccgatc
     1561 acgttacctt cgtcatcttt gtacacttct gcaagttgtg tttctgtaaa gaattcttcg
     1621 tcagaaacag agtaccagcc ttcatattca cctaagtaaa tatctccctg ttctaataaa
     1681 cgatcaaata ttttttgaac agctgcttta tggtaatcat ctgtcgtgcg aatgaattta
     1741 tcataactga tatcaagcgt tttccataat ttcttgacat cagccgccat tttatctaca
     1801 tattcttgcg gttttacacc tagttcttct gcttttgttt cgattttttg accatgttca
     1861 tccactccag tgagataaaa cacatcaaag cccatcaacc gtttataacg agcaattgcg
     1921 tcacatgcaa tcgtagtata cgagttgccg atatgaagtt tgccgcttgg ataatagatt
     1981 ggcgtagtga tataaaaagt ttccttttct gccacggggc tcttcctcct ttatcgcatg
     2041 atgcgaattc gtttccttgc tagtatacca taaaaaaagc atgctgttag ctttttttca
     2101 aaatgaaaaa gactggataa acgacttcta tctacgttca atctatcctt taaaaggatg
     2161 aataggttga ataaatgcgg gtttttgttt tgtttcatat ctactaaaaa acggcttttt
     2221 atttctcccc tcacacgtgg tataataaag actatctatc ttagtaaggg actggaacaa
     2281 tcatgcagaa gaaacatctt ttttttacac ttagtattgc ttttctttca ttagcacatt
     2341 taatattcag ctatttttat attcgtatgt atggttattt taacctccat gggcatttga
     2401 acagttttat gacagcagct tggattttgc gttttatcat tgacgtatat atcgttattt
     2461 gtggcttctt tgccattcgt gaagagcggt ataaagtatt gccgttttat ctgcttttct
     2521 ttttattcaa tctcatactt ccatttattt ttcatattta aatgataacc tagatttttc
     2581 aagtaaatcc ttggtttttt ttcagagtct gctacaataa ggtaaaaagt acttttactg
     2641 tcaatggagg tggtcagatg aaccgttttg cttctaaaga agaagaaaaa ttatattatg
     2701 aacataaagc aagtgaagaa gaatttctta actggtatca aaaacaggaa cgtccagaat
     2761 atgacaagcc ctctttgact gtcgatatcg tactgatgtg ctataacaaa gaagaagatc
     2821 agctgaaagt tttgttgatc caaagaaaag ggcatcctta tcgaaattca tgggcattgc
     2881 ctggtggctt tgtccaaaag gatgagtcaa ctggagaaag cgtcttaaga gaaacaaagg
     2941 aagaaactgg tgtggttatt tcaaaagaaa atattgagca actgcataca ttcagtacgc
     3001 ctaatcgaga tccgcgaggc tgggtcgtca cagtcagcta cttagccttt attggagaag
     3061 aaccgctaat cgccggagat gatgccaagg aagtacgctg gttcacacta gaacgtcata
     3121 gtaacaagat ccatttatct agcggagaag tggctatctc gcttgattta gtcactggag
     3181 agtcttctgg caaagatact ttggcttttg accatagtca aatcatcttg aaagcattca
     3241 accgtgtagt caataaaatg gaacacgagc cgcaagttct acaagtttta ggtaaagatt
     3301 tcacgattac ggaagctcga aaagtatttg ctaaattctt agggatcgat tataaaacga
     3361 tcgatcattc caattttaag aaagctatgc tccaatattt cgatgagatc ggtgaacgtc
     3421 ctgtaggaat cggccgtcct tcaaaaatct atcagctcaa gccggggcac catgaataag
     3481 gttgtgagca aaagattcag acctaatcat tcaagaaaaa acgcaaaaac aactctttgt
     3541 gagttacgtt ttttgtttca gaattttgat gtattcgtaa actttctgaa taaaaaatta
     3601 acaatagcag aaaaagctcg tacgaacttt ttctgctatt gttttattgg tttgtcataa
     3661 ttagtgagct tttcttcaaa agaaaaattc ctagaaacaa aagtcattaa gactttttat
     3721 taacaaggct aaccctaacg ctcctatcag tttcattcat tttttatccc ttgataaccg
     3781 aatgtctgcc cattttttct actacttggc tctttcttac ggaattgctt tcttctgctc
     3841 cgtcggtatt gatcaaaaga aattttttat tataccattg ggacttataa cgcaattttg
     3901 ttattaattt atgccagtcg tgaatagaaa aatcagcatg tgcctcttct tttggaaaag
     3961 attctacaaa taagtagtca tattgtttga ccaatttccc agataattgt tcaagataat
     4021 cttctcgctg gttcttttgt ttcagataaa gatccattac ttttctcttt tgtttctgat
     4081 aattgcttgc ctgatctaac ctgatctttc tatggtgagc ggagcggact ttcacattca
     4141 atttccgctg tgcatgttgc agcttttctt ctgtctggtg aaattttggt aaagttactt
     4201 ctagaggtcg agatgtttgg atcaatgtct tcggatgata ggcgatcccg atccatttag
     4261 ctaaagattc tttttttact ggcgaaacat cacaaagtag tgcgacataa aattcttcat
     4321 tatatcgagc agaaatcgta gcggagcgaa tcgatccttc aactggccga tgctgattta
     4381 tcttgattag ttctttttgt tttggtaact ttaagtagcc attttccaga tagatcgtac
     4441 gggtttggtt gttggtcgta taagattgcc acatactttt tttcgtttta agctttggga
     4501 agctggcacg tcccttgaaa taattgcgaa aagcccgatc caaattaagc tgtgcatttg
     4561 ctaaagcaag actgtctact tcttttaaga atgggtattc tttttttaat gtagcgggtg
     4621 tgagcgaaaa attggctact cctgtttgtt gccaactttt ctgccgcttt ttcagcaaat
     4681 ggttataggt aaatcgagca catccaaaag tacgaatcaa ttgctgtttt tgttcttctg
     4741 tcggatacat acggaattta aaagctttta gcatctatgt ccctcctagt tagcaaccag
     4801 aagagattcc ttttgtcgtt ttagtttttt tgcgtaatat gggacttcct gctcagttgc
     4861 atagacaaaa tgttctggtg cgatttcacg aagttgacgt acttgtccgt catctggttc
     4921 aggttgatat ttgattcttc tgcgttttct ctcatggtct ggatctggta gaggaatagc
     4981 agataacaaa ctttctgtat aaggatgaac accgtgatta tagatttcat cacttgttcc
     5041 catctctaat aacaagccgt tatgcattac accgattcgg tcactaatat gtttaaccat
     5101 cgacaaatca tgtgcaataa atagataggt caattgatgt tctttttgga gatcttgcaa
     5161 caaattgacc acttgggctt ggatcgacac atctagtgca gaaattggtt catcacaaat
     5221 gataaaacgc ggttttactg ccaatgctcg tgcaatacca atacgctggc gttgcccacc
     5281 tgaaaattca tgaggataac gagtaccgtg gcttggattc aatccaacag ttctcaatag
     5341 atcatcgact ttttttgcac gctcttcaga tgatttagct agtccattaa catcaattcc
     5401 ttcggcaatg atatctctga ctttcatacg aggattcaac gaagcgtatg gatcttgaaa
     5461 gatcatctga acatcatgcc ggaatttcgt caaacctttt ttatctttga tcttcgttat
     5521 atcttgaccg tcgaataata tctcaccatc agtaggttca ttcaagcgaa tgatcgtacg
     5581 gccagtagtg gattttccgc ttccagattc tccaactaaa ccgaatgttt ctccttcata
     5641 aatgtgaaag ctgatgtcat ttacagcatg tacttcattc ttttttccta cattgaaata
     5701 ctgtttcaat cctttaactt caagtagttt atttggcatc agcaattcct ccttgaccgt
     5761 aagtatgttt ttgtttctct ttaaagatag cccagcgacg ttggatttct tctggcggtg
     5821 tcactttggg tgcctgcgga gcaagtagcc aagttgctgc cttgtgtgtt tttgatagtt
     5881 caaaaaatgg tggttcttgt tctgcatcga ttttcatcgc gaattcattt cttggataga
     5941 atgcgtctcc ttttggcggg ttcaataaat ccggtggaga accaggaata gcatacaatc
     6001 gatcgttcgc actatctaat gtcggcattg aaccaagcag tccccaagta tagggatgtt
     6061 gcgggttgta gaaaatttct tccgatgttc caacttcaac gattcttcct ccatacatga
     6121 cagcgacacg atcagcgaca ttcgctacta caccaaggtc atgagtgata aagataattg
     6181 atgtatcgat ttttgtctga atttctttca gcagctccaa aatctgtgct tgaatagtca
     6241 catcaagcgc tgttgtaggt tcatcagcaa tcaggatttc cggataacat accagtgcaa
     6301 tcgcgatcac gatccgttga cgctgacctc ctgagaactg atgcggataa ctgttgatac
     6361 gtttctcagc ttcgggtatt ccaactaatt taagcaattc taatgcagct ttttgtccct
     6421 ctttttttga aactttatta tgctttctaa gggactcagc gacttgcttc ccaatgggca
     6481 tcgtcggatc taaagaggtc attggatctt ggaaaatcat cgctatttct ttaccacgga
     6541 ttttttgcat ctctctttct gattttccaa cgatatcgtt acctttaaag aggatttgac
     6601 cagcatctat attggcgttg cttgacagca agcgcatgat gcttctggtc gtcactgatt
     6661 ttccacttcc agattcccca acgatcgcca atgtttctcc tttattcaaa tgaaaattga
     6721 caccgcgaat cgctcgtacc tttccagcat acgtatcaaa agagatttct aagtctttca
     6781 cttctaaaat tttcgtcatc gtcttcactc tttcatttta ggatcaaagg catcacgcaa
     6841 tccatcagca agtaagttaa agccaatcat gataaccgat aaagttactg ccggaatcca
     6901 taatagataa ggcagataca ggaatgtctt gtacccttcg ctcaacatcg ttcctaatga
     6961 agctgacgga ggcgtaagac ctaaaccgat aaagcttaaa aaggcttcga agaaaatagc
     7021 agatggaata ctaaacatca tttgaataat aatgacacta gagatattcg gcaaaatatg
     7081 tttgaatgca attttcattc ttgattcacc taatgtcaat ccagctaaaa catattcttg
     7141 atcttttaat ttcattgttt gtgcacgaac gatacgtgcc attggtatcc agtttgtgat
     7201 ggccatagca gccacgattg aaaaaattcc tggttcaaaa acgaccaaca tcaaaatcat
     7261 gacaacgagg tttggaattc ctgataagat ttccaaaaaa cgttgcatca cgttatcgac
     7321 acgtccgcct aacaaaccag aaaccaaccc gtaagctaca ccaatcgtta catctagtaa
     7381 tgcagcaata aatgcgatca gcaacgagat tctcattccc ataaataagc ggcttaatac
     7441 atcacgtccc aaaccgtctg ttcctaaaaa gaaatttacg tttgaaggga cgtttgcttg
     7501 agcatattta tcaacaagtt caccaccaac cattgctttt ccattcaatc catcgatatt
     7561 gattcccgga atacgaggag gcaaattgat ataagccacg ttttgggcag ttggatcatg
     7621 aggagaaaca aagattgtga tcacagagat caaaatgatg accaatagta aaaccattga
     7681 aataacggca gctttgtttt tctttaaacg gcgccatgaa tcttggataa aattcaaaga
     7741 aggtgcagta atctgttcac gttcttcttc ggtatttttt tgtaatggaa gaaattcatc
     7801 cgcagggata tctgcaactg tttcataacg ctttggtatc atttccattt atcctcggct
     7861 cccttctgaa acacgaattc ttggatcaac tagaccgtac aatagatcca cgatcaaaat
     7921 aacaaaaatc agcataaatg agtaaaggat cgttacagcc atgatcgtag gataatcatt
     7981 tgttgtgatt gacttaacga attgttcccc gataccagga atagcaaaga tattttctac
     8041 caccagtgat ccggtcatca atgctacagc taacggtcct aataaagtca tcaatggaat
     8101 caaactattt cgaagaccat gtttaaaggc gatttcccaa cggctcaatc cttttgcttt
     8161 tgctaattcc acataatcac tatgcagtac ttccaccatt tccgtacgga taaaccgagc
     8221 agagtcagca agcggcgaca tggccaacgc aattgttggt aagattgtgt acacaaagcc
     8281 atcccatttt gcgattggca aaacttgtaa tttgattgca aagatatatt gtaataaaac
     8341 agcaaataca aagtttggaa tggaacgacc taatatcgct acgagtgtag aagatgtatc
     8401 tgcccatgta ttttgcttca tggcagagat cgttcctaaa atcgtcccta ggactgttcc
     8461 aaagataatt gcctgtaacc ctaactgcaa ggatggtccg attcttccag ctaacaaatg
     8521 tgcgactggc tggtttttaa attggaagga aattccaaaa tctccttgaa gcaaattaga
     8581 aagataaatc ccatattgta cgataactgg tttatccaaa ccgacttgtt tatttagcat
     8641 ctcgattgtt tcaggactca aacgttcctg attcgtatat ggcgtgcccg gcagcaattg
     8701 catcaagaaa aaggtgattg ttgcaattaa ccaaagcgtg atgatcatga aaaagacacg
     8761 ttttaataca tatttgatat aactattcac taatgtttcc ccctagcttt tgacttctga
     8821 cagatgtttc gctacattat tagataatga accaaaaata aaggagcaat caaatgaaaa
     8881 aaaaatctat cccctacgca gtagcttttc tactgattct ggttatttta ataaaaaatc
     8941 tcatcaacca ttcctttaca ctcattcaat tatctaatga tctattctta tggtcattgc
     9001 cgtttctgat tatcggcggt tttttatggg tcttttcttc gggtttcttc gatcattttc
     9061 agcgatcggt ccatcttgca cgaacaagga accgaaagaa aaaaccagaa ttttcctcgc
     9121 tctcgtctgc cagctacggt atgtacagct tttggctgat cattgccgga atactaattg
     9181 ctctttctgc tattttcatg cttttttctt tgttaggata agtgaatatg agaacgaggt
     9241 tgcgacattt gccgcagcct cgttttttct catgctcacg gtattaacga ttattcttca
     9301 acgtatgtcc atttgtaatc ccatgaagcg cctgccgcat ggtgaacgat acctttgatt
     9361 ttttcattca ctaggtgacc ttctgctttt tggtacaccg gaacgacacc catatcatca
     9421 gagatgatct tgattgcttt ttggaaatca tcccaacggg cttgttcatc gccagcgtct
     9481 gttgatgaag ctgctttaac tgcttcgtca tattctgcat tgctccagcg accacggttg
     9541 tatgaattac ctgtgacaaa caaatcagta aagctgcttg ggtctgcata gtcagcagcc
     9601 catccgccta gtactgcgtc aaaatcaccc gaagttgagc ggtcaagacg tacagagaat
     9661 ggaacaggtg ttggtttgac tgttacgcca tccaagtttt cttggattga gttttgaaca
     9721 tattcgatta cttttttcga tgaatcatca tcagaagcca tcaaatcaaa ttctaaagaa
     9781 tcaacgccca attctttttt cgctttttcc caatattctt ttgctttatc tttgtcataa
     9841 gaaactaatt ttccagcttc ttttgcaaag tcttcgttcg ttgttggact ttttaccata
     9901 tctgatggga tcaatcctgt agagacaact gatccgtcac ctagtacttg ttttactaaa
     9961 gcttcacgat cgatagaata agaaatagct tttcttaggt tcacattgtt gaatggtgaa
    10021 ttcttttcac gttgattgaa ttcgatatag cttgtccgcg cttctttaac agaagtataa
    10081 gctggatcgt ttgcgttttg ctgtgctaac tcaccagaca agatcacgtc atctgcttgg
    10141 ccatctttga ataagttcaa tgcagtagaa gattctttta ctacactgac tttgatttca
    10201 gaaagtttta ctgtatcttt gtcccagtaa tgatcatttt tcttataagt ccattctgta
    10261 tctgttcctg gtccatcaaa atcttctaat gtgaatggtc cattataaac agctttatca
    10321 cttgcagatg cataggcatc cccattttct tccactacgg attgtttttg tgggaaaaat
    10381 gatgggaaag caagcagata ttggaaatat ggtgtttgtt tcgtcaattt gatttctaat
    10441 tcatagtcac caactgcttt gatccctagt tctgatttat ctttctttcc agctgtaatg
    10501 tcttctgcat tttcaactgg cgcatacagg taagcatatt cagaagctgt agctgggtct
    10561 actgttcgtt gccagccata aacgtaatcc gctgctgtaa caggatcacc atttgaccat
    10621 tttgcgtctt ctcgtaattt gattttgtaa gttaatccgt cttcactgac ttctgccaac
    10681 tcgctagctc ctgccggctg tggtttgctg tcttcatcaa gacggtaaat tccttcataa
    10741 acgttattca atgctgtaaa actgattgta tctgttgcaa ctgataagtc agctgtaggc
    10801 atttcttgag gcacgactaa gttaaatacg cctcctccat ctgctgaacc gctttttgct
    10861 gaactactgc ttgtggctga actgccgcca taacatccag caagtacaag gccgcatacc
    10921 gcaaccaaac caaatgtaaa tttctttttc attttacgta cccctttatt ggaattttca
    10981 gaataattaa tattttctga agttgtttaa gagtttaatg aataattatt aaaaaatcaa
    11041 cacttttttt tgtctttttc ataaaataat gattttttcg tgag
//
LOCUS       DAXWOO010000090        11055 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.090, whole genome shotgun sequence.
ACCESSION   DAXWOO010000090 DAXWOO010000000
VERSION     DAXWOO010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11055
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.090"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..891
                     /locus_tag="ACGE70_001832"
     CDS             <1..891
                     /locus_tag="ACGE70_001832"
                     /inference="COORDINATES: protein motif:HMM:NF025298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFF8693462.1"
                     /translation="HNKRNGGMKKKKFSRSLLGILVLVAVGLSGKGYFQNNSTQEHDF
                     QIKSSVQVGNKADSESKEFLGIRYDPNKYPNNIVNINDGKYTLTKEDHEALSEKGSQT
                     FWVNYADLDQLGRAGDVEALITYDAVNSHSSKNVERPSFKSSVHIAGEYKDGVFDQER
                     DTWVGKDSNNEITQLEGYKGYLYNKSHSLAWSLGGDMEVHNLTLGTRAQNVGKNDQNG
                     GMAYSETIVRDAIYKNKNEKVLYKVDPIYKGNELVPRGSHVEAFSVNDNGKTVNLNVY
                     VFNNQKGININYQNGTWVRD"
     gene            911..1258
                     /locus_tag="ACGE70_001833"
     CDS             911..1258
                     /locus_tag="ACGE70_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693463.1"
                     /translation="METISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMIIEREMLDEIRVIIPKDTQMCE
                     DSISDDEPKSERK"
     gene            1271..2533
                     /locus_tag="ACGE70_001834"
     CDS             1271..2533
                     /locus_tag="ACGE70_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693464.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVASFSKSVYLVGRALVVGAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATSGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETERATSSIAVSNSQFSFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            2613..3989
                     /locus_tag="ACGE70_001835"
     CDS             2613..3989
                     /locus_tag="ACGE70_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFF8693465.1"
                     /translation="MAMKLLEKVKKRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKD
                     PTDAIFGIKPDGTIAHFCISEMPHMLVAGTTGSGKSVLLNEILVTVMCHSTPDELKIG
                     IVDPKRVEFGRYKKLPFMLADPITDMDEAYDFFEYLVILMHERYKLMEKAGLQNITLY
                     NEYAEKNGLDRFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMLIKLVDGSMVRAQG
                     AYISNKEIETIFNHLRNTMPEPEYVDYRAIVAETRGEGGEDEDFGGIGKAPVRKSAKR
                     NLNTPLANPSSKEPSIEKKSAFAIKLEKNKEEKVEQGAKTLEKLQAEKLEKVLRALGK
                     ADPEPQTSNNTNNKQPIASKTKPKANDPKGSENVVITTDKSEKKQINGSTSPIMQKML
                     KAKKQVHK"
     gene            3986..4615
                     /locus_tag="ACGE70_001836"
     CDS             3986..4615
                     /locus_tag="ACGE70_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693466.1"
                     /translation="MIIVSSFLRWLTPYKYTDEVVVKRAKKRDKKAVLTPKQMLDHGI
                     LGVKVLEVNKPFESNSAREKLLGAEADVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLRPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSNQNRVQINKFEKKVRK
                     FNDRKTILWFADICKQPDGTAKWEAVIFDENGKKVMSETWHDKAKFVWQ"
     gene            4685..5113
                     /locus_tag="ACGE70_001837"
     CDS             4685..5113
                     /locus_tag="ACGE70_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693467.1"
                     /translation="MGETIEYKDKNYIIIGIYNTRINRIGDPRITSDLVCQSVNYSPD
                     LTSRYKKYVLLEYRHKITDEIGVNNTRNIFKIGTVIPYSNNEEKIFYQIYGIEKFEYE
                     HVDLLVTYQMRLIEPWSQAEIDKAVKLNRLSKFNVLGNAF"
     gene            5672..6271
                     /locus_tag="ACGE70_001838"
     CDS             5672..6271
                     /locus_tag="ACGE70_001838"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFF8693468.1"
                     /translation="MREKNKNSSRYLVATVVFVCVLIFLFDFLQIGKSIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGFLGSLVPFLLHSNIVSMGLSGGIFGWIALFAIAYIRFDSKKHLLLSVFVLLIIGIL
                     LVSTFESGEANVLSHVIGFVCGMILGFILKNVSLIKQKN"
     gene            6287..6763
                     /locus_tag="ACGE70_001839"
     CDS             6287..6763
                     /locus_tag="ACGE70_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693469.1"
                     /translation="MPKRKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMYE
                     QREALRERIHYFDDIHTKIYEARTNHEDESKLGLAQQRILEWLNTNTDQEPLRVISNL
                     FEFGTKHWFNAVEKDADLQEAFDTLSKDSKSMRDLLITYLNEHEFEQTKTKKKNKE"
     gene            6780..7682
                     /locus_tag="ACGE70_001840"
     CDS             6780..7682
                     /locus_tag="ACGE70_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693470.1"
                     /translation="MLLTLLGYLCLILAFFGAIAIIIGQFQTNNPIMRNIGIGIVIVT
                     FIVFNATKIFGSNDKQFGISGVNNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDGK
                     KASDDVLATIKPSQSQFEVFYTVKENSPVHKLTLVFNVDGEEYYKDVKINKTSTKKII
                     ESAKKEVATEESTTESSSTVEAELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQMINVTLFNSVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGADLPVH
                     VVYEDGKVFGDSTANEALTIKLAK"
     gene            7755..7946
                     /locus_tag="ACGE70_001841"
     CDS             7755..7946
                     /locus_tag="ACGE70_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693471.1"
                     /translation="MVVNKEKVRKYVTLKIASVKYLEKRAKEEERARGKKVTVSELLE
                     ELIENDQTIRRAFTNRSIR"
     gene            8110..8697
                     /locus_tag="ACGE70_001842"
     CDS             8110..8697
                     /locus_tag="ACGE70_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693472.1"
                     /translation="MENNNDIRHFSIGERVTTVSELSKDYRDKEGTIISLNKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNNGSHSIEKAILVYPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITSKEAKIEDVLTVRELQSLIEYRNKQITNPEYLRKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDTFKKIYLKEIKKD"
     gene            8697..9596
                     /locus_tag="ACGE70_001843"
     CDS             8697..9596
                     /locus_tag="ACGE70_001843"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0000400 - four-way junction DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFF8693473.1"
                     /translation="MWTVTLPFQPVSAPRPSARRNAADETNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQDLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            9596..10510
                     /locus_tag="ACGE70_001844"
     CDS             9596..10510
                     /locus_tag="ACGE70_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693474.1"
                     /translation="MNNNEKILHVLDSFEIIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLVERKEQEKLIKAYFELGEKEVKNAMELNEERRVLDQLLEQLLVMFQ
                     KGRIDEDLIEEGLRKYPSNSGIGIVLKAIDEEEIEAFIPPEDFESAMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCESYGVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     EARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNATNRLEKAVSELDA
                     YYREHYLQAGGMPRNTKANISRYIKKAE"
     gene            10562..10777
                     /locus_tag="ACGE70_001845"
     CDS             10562..10777
                     /locus_tag="ACGE70_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693475.1"
                     /translation="MKRDVFELYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NSVVLKQFWLNNFSYKDFIKEKANTLY"
BASE COUNT         4243 a         1497 c         2252 g         3063 t
ORIGIN      
        1 cacaataaaa ggaatggtgg tatgaaaaag aagaagttta gccgatcgtt gttaggaatt
       61 ttggttttag ttgcggttgg tcttagtggc aagggttact ttcagaataa cagtactcag
      121 gaacatgatt ttcagatcaa aagtagtgtt caggtaggta ataaagcaga cagtgaatca
      181 aaagaatttt tagggatacg ttatgatcca aacaaatatc caaataatat cgtaaacatt
      241 aatgatggga aatatacact gacaaaagaa gaccatgaag cattatcaga aaaaggcagt
      301 caaacctttt gggtaaatta tgcagattta gatcaacttg gaagagcagg agatgttgaa
      361 gcgttgatca cctatgatgc tgtaaattct cactcatcta aaaatgtgga acgtccatct
      421 ttcaaaagtt ctgtccatat tgcaggagaa tacaaggatg gagtatttga tcaagagaga
      481 gatacttggg tcggcaaaga ttcaaacaat gaaatcactc agctagaagg ctataaaggt
      541 tatctctata acaaaagtca ttcattagca tggtcgctag gaggagatat ggaggtacac
      601 aatttaacac ttggaacgag agcgcaaaat gttgggaaaa atgatcagaa tggagggatg
      661 gcttactcag aaacgattgt acgtgatgca atctataaaa ataagaatga aaaagtattg
      721 tataaagttg atccaattta taaagggaat gaattagttc ctcgtggttc tcatgtcgaa
      781 gctttctcag ttaacgataa tggaaaaacg gtaaacctta atgtttatgt atttaacaat
      841 caaaaaggaa tcaacatcaa ctatcaaaat ggtacttggg taagagatta gaaattgaga
      901 aggtgaagaa ttggaaacaa taagcatcaa tggattagat aaatcagttg caaatcgaat
      961 aaaaaaagga aatggctact atcttctgaa gaagggtaaa tattcagttg ctagagagtt
     1021 agaaacaatg agtatcgtgt atgtagctaa gaatcatttt gacgtagaac aggctatttt
     1081 tgatccagat tctgaaatag ggaaatgtgt tgaattatca gagaaaactg ttagtacaaa
     1141 aggggcgatg atcattgaga gagaaatgtt agacgaaatc agagtaatca ttcctaaaga
     1201 tacgcagatg tgtgaggaca gcataagtga cgatgaacca aaatcagaaa gaaaataagg
     1261 agatattcag atgagcgaaa aatccattga attaaaggaa tataagcgaa gcgatggaga
     1321 aaaatattat cctgtagcca gtttttctaa atcagtatat ctggttggac gtgcattggt
     1381 agttggtgca attggcatat atattttgca ttgggtaaca aaggttgtac aactgcttgt
     1441 agatacctta gtaagtattc ttgtctcgtt aaatgatgtt aacggaacag atgatttagg
     1501 attaaaagag atgtttaaag agttactcaa tgcgtttttt cccaattatt taagttattc
     1561 atataatgta caaagctttt ttgcaaagct acacttggta gacatctcta ttttagtatt
     1621 agaagcagga acggtattct ttgttggata tttggttact ttgatgttca ggcatcacta
     1681 tggagaacaa agaccatttt ttgatgatat ggaaagtaga aggttgaaaa gagaagctct
     1741 agaagctatg gatgtgaagt atgatccaaa caagattata ggagatgaaa cgaacaacaa
     1801 aaaaagaaaa cgaaacaaaa cgcaattaga atccgatgtg ctatcaacgc ttaaaaatat
     1861 caaaatttct gttcatacaa gacgtgatct aagtgtcaat gaagatattc gagaatatat
     1921 cgtaaaaatc ccgcaaccaa aagggaagaa agttagagaa acagttttac gacaaattca
     1981 agatttaggg tcgatcctga caagagcaac aagcggacga gccaaatttg gtgaaaaaga
     2041 agatgaagtt tcaggcgatt atttcatttt ccgagcatca gtaaatattg aaagagaacg
     2101 taaggaaaga gtaagtacca aaaacaaaga aacggaaaga gcaacatctt caatcgcagt
     2161 ttcaaattca caattttctt ttccaatcaa tctctttgcc gataattcca aaaatatcca
     2221 aaaccaaacc gaaaaagcta gaaaatatgc cgaaactcaa aagcaatctt tagaacttta
     2281 tctatcttct gctgacgtac ctgcaagtct aacagatatg cacgttggga atacatctat
     2341 tgaatatttg tttactttat ctcgtaccag aaaaattcaa ctaagctcaa tgaaagaaga
     2401 aattgaaact tacttgcata tatcaggtgt aatcgtgcgg atcaacgcag ggcgtattca
     2461 aattacttta ccattaggag aaaatgatcg tattccaatt gatgtaccat caatgatcaa
     2521 tagcgtgttt tagaagacat cacaaataga aacagaaaat acaaactacc agttcacaaa
     2581 cagaaaagta ccgagaaaag tgaggaatag aaatggctat gaaattatta gagaaagtca
     2641 aaaagagagc cgaaaaagct gaatctattt tgctagataa agaacctaat ataaagctta
     2701 atggaaaatt cagtgtcaat atgttgaaca caaaggctaa agacccaaca gatgctattt
     2761 ttggaattaa gcctgatggc acaattgctc atttttgcat cagcgaaatg ccacacatgt
     2821 tggttgcagg aacgacagga tcagggaaat cagtattact gaatgaaatt cttgttacag
     2881 taatgtgtca ctcaacgcct gatgaattaa aaataggaat tgtagaccca aaaagggttg
     2941 aatttggaag atacaaaaaa ttgccgttca tgttggctga tccaattaca gatatggatg
     3001 aagcttatga tttttttgaa tatcttgtga tcttgatgca tgaacgttac aaactgatgg
     3061 aaaaagcagg gttacaaaac atcacgctat ataacgaata tgccgaaaaa aatggattag
     3121 atagatttcc ctatgtgatc ttattagttg atgaatattc acagttggtt ggaacacata
     3181 aagaagtaga aggtttgatt gttcaattag gacaaatggc aagggcagca ggtattcatg
     3241 ttatcttagc aacacaatct cctcgatcaa cagttgtcac tggtattatc aaagccaatt
     3301 ttccgtcacg tgtttgcctt atggtagcaa gtgatttaga aagtaggatt gtattagatg
     3361 aaggcggtgg agaaagtcta agtccaaagg gagatatgct tattaagctt gttgatggaa
     3421 gtatggttag agcgcaggga gcttacatca gtaacaaaga aattgaaaca atcttcaatc
     3481 accttagaaa tactatgcca gaaccagaat atgttgatta ccgagcaatc gtagctgaaa
     3541 ctcgtggtga gggcggtgaa gacgaggatt ttggtggaat tggtaaagct cctgtaagaa
     3601 aatctgctaa aagaaatttg aacacacctt tagcaaatcc atcatctaaa gagccatcaa
     3661 ttgaaaagaa gagtgcgttt gctattaagc ttgaaaagaa caaagaagaa aaagtcgagc
     3721 agggagctaa aacactagaa aaactgcaag cagaaaaact agaaaaagtt ttaagggcat
     3781 taggaaaagc tgatcctgaa ccgcaaacct caaacaacac gaataataaa cagccgattg
     3841 catctaaaac caaacctaaa gctaatgatc caaaaggctc agaaaacgta gtgatcacaa
     3901 ctgataaatc tgaaaagaaa caaataaatg gaagtacttc acctatcatg caaaaaatgt
     3961 taaaagcaaa aaaacaggtg cataaatgat catcgtcagt tcttttttaa gatggcttac
     4021 tccatataaa tatacagatg aagtagttgt gaaacgagcg aagaaaagag ataaaaaagc
     4081 ggtactaact cctaaacaga tgcttgatca tggcatttta ggggtgaagg ttttagaagt
     4141 gaataaaccg tttgaatcaa atagtgcaag agaaaagttg cttggagctg aagccgatgt
     4201 cgcatatttt taccatgaca aaagcaagca tcaaatagct attaaattca atccaaaatc
     4261 ggcatggcgg tatcaagcaa gagatgactt gaaacgtagg aaacttagac caatgctcta
     4321 tccagaagga aaagaagcat ttgatagaac ccacatcgtt cccattggtt ttcatggtac
     4381 agaatcagat aaccgcctat tagtaggttg gaacagcaat caaaatagag ttcagatcaa
     4441 taaatttgag aagaaagtaa ggaaattcaa cgatcgaaaa acgatcctat ggtttgcaga
     4501 tatttgtaaa cagccggatg gaacagcaaa gtgggaagcg gtgatatttg acgaaaatgg
     4561 taaaaaagta atgtctgaaa catggcatga taaagctaaa tttgtttggc agtagcagta
     4621 gaggtgaaaa agattgctag aactttttga agcaaataga acaggcttta atcaaaagtt
     4681 agaagtggga gaaactatag aatataaaga taaaaactat attattatcg gaatctataa
     4741 cacgagaatt aacagaatag gtgatccacg tataaccagt gacttggttt gtcaatctgt
     4801 taactattca cctgatctga caagcaggta taagaaatac gtgttattag aatatagaca
     4861 caagattact gatgaaattg gtgtaaacaa cacacgaaac atatttaaga tcggaacagt
     4921 gattccttat agtaacaacg aagaaaaaat attttatcag atttacggaa tagaaaagtt
     4981 tgaatatgaa catgtcgatc tgctagtgac gtaccaaatg cgtttaatag aaccttggtc
     5041 acaggcagaa attgataaag cagtaaaatt gaaccgatta tcaaaattta acgtattagg
     5101 aaatgccttt taaaaatcgc aatacacaaa cagaaaaata ccaaaaaatt agcaatgaac
     5161 gtaggtaaga acatgacaat aaataataat aaccctaaaa aatataatgc tcttgtccaa
     5221 tgctatatcg aaaacaaaat tacaatgcaa gaagtcgctg atgaattagg caaatcaaag
     5281 agcctagtat cacacgattt taagcacaat gtttttccga acccgattga gctgaggaaa
     5341 aaatttcaaa agagagttta tcaagaaata ggtaagtgga ttgatgaagg gaatgcaatt
     5401 gaccctttaa ttgaaaaata tcctttttta aaaagcaaaa ataaaattca aaggttaatt
     5461 cgtcaaggcg atattcaagc taaagaacct gttttgacaa ccaatgattt ggctaaaata
     5521 aatcttcata aacaaattat tgaagacttt gaaaaaaatc tttcaacaac tgaaatttta
     5581 aaaaaataca atattccgag aggaaagcta acatcgactc tttttagaga atatggagaa
     5641 tcggcagtta ctaaagcaaa ggaagtagcg aatgagagaa aaaaacaaaa atagtagtcg
     5701 atacctagta gcgactgtag tttttgtttg tgtattgatt ttcctgtttg attttttgca
     5761 gattggaaaa agcataattg gaaatacatt gagtgcaaag gaaatggttg ctttaggtgg
     5821 tttgtatgaa ggacacttgt tcccgaatat attcatttct atcgtttctc atattaactt
     5881 gtaccattta atttctaatt tattgttctt agtagttctt gaagaagcac tgaatgatca
     5941 gtatgagtgg ttaacattat tgatcgtttt aatcatatca ggatttctag gaagcttagt
     6001 tccttttctg ctacacagca acatcgtaag tatggggctt agtggaggta tttttggatg
     6061 gattgcttta tttgcgattg catacatccg ttttgatagc aagaaacatt tattactaag
     6121 tgtttttgta ttattgataa tcggcatttt attggtatct acttttgaat caggcgaggc
     6181 aaatgtcttg tcacatgtaa tcggatttgt ctgtggaatg atcttgggat ttattctaaa
     6241 gaacgtttca ttgatcaaac aaaagaatta aaagggagtg ctaaaaatgc caaaaagaaa
     6301 agaaaagaaa attccagtgt ttttgtctgt ctcagaaatg ttatcacttc aaatactgtt
     6361 aagaaaaaag attgaaacaa tagacgaaga agttagaaat aaaagggcta tgtacgaaca
     6421 acgtgaagcg ctaagagaac gaatccacta ttttgatgat atacacacta aaatctatga
     6481 agcacgtaca aatcacgaag acgaaagtaa attaggatta gctcaacaac ggatacttga
     6541 atggcttaat acgaatactg atcaagaacc gttaagagtg attagtaatc tttttgaatt
     6601 tggaacgaag cattggttta atgctgtaga gaaggatgct gacttacaag aagcgtttga
     6661 tacgctaagt aaggatagca aatccatgcg agatttattg attacttatc taaatgagca
     6721 tgagtttgaa caaacgaaaa ccaaaaagaa aaataaagaa tagaaagttg tgatgaatca
     6781 tgctattaac cctgttaggt tatttatgtt taattcttgc tttttttggg gcaatcgcta
     6841 taattattgg tcaatttcaa acaaataatc cgattatgcg taacataggc attggaattg
     6901 tgattgtaac ctttattgtg tttaatgcca ctaagatttt tggaagtaac gataaacaat
     6961 ttggtatatc gggagttaat aatagttatg ctccagattc tttgggaagg gtagtaatag
     7021 atggaacatt tgaaaatgta acgagttata aaaatgtttc gctgacagtg aaggaagatg
     7081 gcaagaaagc atcagacgac gttctcgcta ctattaagcc gtctcaatca caatttgaag
     7141 tgttttatac agttaaagag aatagccctg tacacaagct gacattggtt tttaacgttg
     7201 atggagaaga atactataaa gatgtaaaaa taaataaaac atcaacgaaa aagattatcg
     7261 aatcagcgaa aaaagaagta gctacagaag aaagtacaac tgaatcaagt tctactgtcg
     7321 aagcggaact accagataaa attgattttg atcctgaaaa agtcaaatcg tttgaatctg
     7381 tttctggttc tatggctgat cggtttaaca ctgaagtctt caaagctatc aaaactgtta
     7441 aattggatac tgaaaagcaa atgattaatg ttaccttatt caattcagtt gaatctatgg
     7501 atcaggataa aaaagttacg gttgcaagag aactaggaaa aagagtaatt ggtttgtatc
     7561 gtggtactgc taaaccagat tcagacggtg cagatttgcc agttcatgta gtttatgaag
     7621 acggtaaagt ttttggagat tctacagcaa acgaagcatt aacgattaaa ttggctaaat
     7681 agccagaaag ttataataaa caaatggtgt cacaccaata atatataggt gtgacacctt
     7741 ggagatgata gctgatggta gtaaataaag aaaaagttag aaagtatgtg actttaaaaa
     7801 tagcttctgt taaatattta gaaaagcgag ctaaagaaga agaaagagca agaggtaaga
     7861 aagtaacagt ttcagagttg ttagaagaat tgatcgagaa tgatcaaaca ataagacgtg
     7921 catttactaa taggtctatc agataacaat ataattaata aaaaagaaag agtgatttgg
     7981 tcttagacta aatcactctt tttatataaa gtttctttac actcattccg aataatgtta
     8041 aaattcttta tatgatatat agttccttgt cagaggaaga gataaccagc aatattggag
     8101 ggataataag tggaaaacaa taatgatatt agacatttta gcattgggga aagagttaca
     8161 acagtaagtg aattatcaaa ggattataga gataaagagg gaacgattat ttctcttaat
     8221 aagaatcgaa gaggcgtttg gcaatatgtt gttgatttag gtgatggcgt tacagttgag
     8281 tgtaaagata gtcaactgat cagaaataat ggcagtcatt ccattgaaaa agcaattctt
     8341 gtatacccaa aagtatcaag taagatcatg aatgactggt tgcttggaaa atttggtgag
     8401 tacaataaaa aagaaatcgc agaagtgatc ggcaaatctt atccaacagt attagggtat
     8461 ctaaaaaaga taacgagtaa agaagcaaaa attgaggatg ttttaacagt aagagagctt
     8521 caatcattaa tcgagtatcg gaataaacaa ataacaaatc ctgagtacct tagaaaagaa
     8581 gatggtctat tgaaacaatt ggaacaagta aataaaatcc ggaagatgca aaaggtagct
     8641 cctttaagcc ttgatacctt taaaaaaatc tatttgaaag agatcaaaaa agactaatgt
     8701 ggactgtaac tttaccgttc caacctgtat ctgcaccaag accaagtgca agaaggaatg
     8761 ctgctgatga aactaatgac gtagcagaga aaaggatttc aacctactat gataaaaagt
     8821 atcttgatta tttgaaaaaa ataaagagct ttatcgaaga tcaagattta ttagatgatg
     8881 aattttactc gattgtaaat gatcctatgg gcgcaattat ggaagtggac ttttactacc
     8941 agataccaaa aagctataaa aaagtctata acattatgaa gacaacagcg ccagatttag
     9001 ataatttagt aaaaagtgct atggatggta tttttaacat ctcagctata cgagatagcc
     9061 atatcacagg cttaattgct tttaaatata atgtggcgaa cgaaggaaaa acagttgtga
     9121 ggatcaaacg atattcagaa tttgagtggg atacttctga aggattgaac ggagatatat
     9181 gggaagctac atttgatttt aaaccagtag caacgccaag accaaagtct aaatttagag
     9241 gaaatgggat tatcacttac tatccaaaag agtacaacga ctatttggaa aatatcaaga
     9301 atacgcttaa agagaaagat ttggtaaatg atcaactgaa aaaggtaatg aatgctccta
     9361 tgggggttat agctcagata gattattttt accaagtgag aaaagataag aaaaagcttg
     9421 attcactgat gcgtacttca cagccggata ttgataattt agtcaaagga acattggacg
     9481 gaatttttaa caaggaaata ggcatcaaag atagcagggt tgtagggtta attgctttta
     9541 aatacaatac ttttgaaaag tcgaaaacaa acgtgagact acaggagatg ggctgatgaa
     9601 taataatgaa aaaattcttc atgtacttga tagctttgaa ataattcaag aagaactaaa
     9661 aaaatatcgg gatgttttgg aacaaagata tgattttgtc aatcagcaaa agagtaatca
     9721 catggatttc attctcaata tgaatgactt gaagaagaaa ttagttgaac gaaaagaaca
     9781 agaaaagttg atcaaagctt actttgaact cggtgaaaaa gaagttaaaa atgcaatgga
     9841 actgaatgaa gagcgcaggg ttttagatca gctgttagaa caattattgg tgatgtttca
     9901 aaaaggacga atagatgagg atttaattga agaaggattg agaaaatatc cttcaaactc
     9961 aggtatcgga atagtgctga aagcgattga tgaagaagaa attgaagctt tcataccgcc
    10021 agaggatttt gaatcggcaa tggaatatat aaaatattat agccaaggga tcactgcttt
    10081 tagagagttt gatcctgaag atgttattca tgacttgaac aacctaaaag aatggtgtga
    10141 aagttatgga gtcgatgatt ccggattgga ctacttgatc agtattatgg aaatcgaaga
    10201 agagatgccg gataagcctg atccaacaga tattttagaa ttgatccatg aagctagaaa
    10261 tccaattgcc tatatttcta gaggatatac tgtattggaa tactataagc cttatatatc
    10321 agcgatgaac catttaagac gtgtattaag ggaaaaacgt gaataccgct cagtgttgaa
    10381 tgcaacgaat agattagaaa aagctgtttc agagctagat gcttattatc gtgaacatta
    10441 cctgcaagca ggtggtatgc caagaaatac gaaagcaaat atcagtaggt atataaaaaa
    10501 agctgagtag gcggagttag cccataaaat ggcaaaaggt aacctgaaag gggcattttg
    10561 aatgaaaagg gatgtttttg aattgtacgt attagaacgt ctagaagacg gcatgatggc
    10621 acaagagata attgatgaaa ttgatgatat gatcagcaga tttggtcaat tggagttgaa
    10681 tgatggaaac aaaaattcgg tagtgctaaa acaattttgg ttgaacaatt tttcgtataa
    10741 ggattttatc aaagaaaagg caaatacatt gtactgacta aattcacgag gagcagtgat
    10801 gagagcttat tgctaagagc agttaggctc ttttttgttt gtgtaaaatt atttgtttta
    10861 taatacacat ttatacacaa aaaaatattg acagatagta cacaaatgtg gtaggatata
    10921 tttgtaagca agttagatta acaagttaaa ggaagagcag ttatgaaaaa agaagaacta
    10981 atagatatat ttcaaattgt tgaacgagca aataatatgg gaattatgtt ttttgatcgt
    11041 atttctttaa agatg
//
LOCUS       DAXWOO010000091        10806 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.091, whole genome shotgun sequence.
ACCESSION   DAXWOO010000091 DAXWOO010000000
VERSION     DAXWOO010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10806
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.091"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(40..1005)
                     /gene="nrdF"
                     /locus_tag="ACGE70_001846"
     CDS             complement(40..1005)
                     /gene="nrdF"
                     /locus_tag="ACGE70_001846"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFF8693476.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(1055..3214)
                     /gene="nrdE"
                     /locus_tag="ACGE70_001847"
     CDS             complement(1055..3214)
                     /gene="nrdE"
                     /locus_tag="ACGE70_001847"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFF8693477.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            complement(3373..3597)
                     /gene="nrdH"
                     /locus_tag="ACGE70_001848"
     CDS             complement(3373..3597)
                     /gene="nrdH"
                     /locus_tag="ACGE70_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFF8693478.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            3998..4471
                     /locus_tag="ACGE70_001849"
     CDS             3998..4471
                     /locus_tag="ACGE70_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFF8693479.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            4468..6600
                     /gene="feoB"
                     /locus_tag="ACGE70_001850"
     CDS             4468..6600
                     /gene="feoB"
                     /locus_tag="ACGE70_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFF8693480.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            6602..6736
                     /locus_tag="ACGE70_001851"
     CDS             6602..6736
                     /locus_tag="ACGE70_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFF8693481.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            6830..7474
                     /locus_tag="ACGE70_001852"
     CDS             6830..7474
                     /locus_tag="ACGE70_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFF8693482.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            7661..8854
                     /locus_tag="ACGE70_001853"
     CDS             7661..8854
                     /locus_tag="ACGE70_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFF8693483.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            8847..10574
                     /locus_tag="ACGE70_001854"
     CDS             8847..10574
                     /locus_tag="ACGE70_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFF8693484.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
BASE COUNT         3372 a         1932 c         2257 g         3245 t
ORIGIN      
        1 tttttatttt tcgaaaaatt tttgaattaa aataaatcct tattccaatc caatcaaata
       61 atcgtcgtct ttcatcgctt ctacatgacc taacaaatag ccgtttccga cttgtgagaa
      121 gaagtcatgg ttgcttgtgc ctgttgaaat cccgttcatt acgattgggt tgacatcatt
      181 ggcagtatct ggaaagagtg gatccatccc taggttcatc aatgctttgt tggcattata
      241 gcgtaggaat gtcttgactt cttctgtcca gccgattgga tcgtataatt cttctgtata
      301 acgttcttcg ttttcgtaaa gctcataaag aagattgtac atccagtctt ttaacgtttc
      361 ttgttctgct tcaggtaact cgttgaatcc taattggaat ttgtaaccga tgtaggttcc
      421 atgtacagat tcatctcgga tgattagttt gatgatctct gctacgttag ccaatttgtt
      481 gtttcctaaa taataaagag gagtataaaa acctgaatag aacaagaatg tttctaaaaa
      541 gacgctggca attttctttt ctagcggtgt accgtgtttg tagatttcat tgatacgctc
      601 agcttttttt tgcagataag gattcgtatt tgtccagtca aaaatttctt cgatttcttt
      661 tttcgtattc agtgtactaa aaatagaaga ataacttttg gcatgaacgg attccatgaa
      721 ttgaatattg ttcaaaacgg cttcttcatg gggagtgcgg acatctttac gcaactgatc
      781 catgccactt tcagattgga cagtatctag cagtgtcaaa ccgccaaata catgtcctac
      841 tgtatctttt tccaattgag acaatgtacg ccaatcatct aggtcatttg aaagtgggat
      901 acgtgtatct agccaaaatt gctctgtcag cttctcccaa gtggatttat caatgatatc
      961 ttcaatttca ttccagttta ttgcttcata atatgtttct gacatgagaa tgctccttta
     1021 attaaaatat gaataagcga atctatctat ggtattagat cacgcagctt tcacattgat
     1081 tgcttccgat ttcttctgca tcatcagtaa aggtacgtac gtaataaatg gatttgattc
     1141 ctttgtggaa agcgtaatgt cttaaaatat tcaaatcacg tgtcgtttgc ttcgttgttg
     1201 tcttccattc ataaatgcct tctgggattt cagaacgcat gaataaagtc aaactcatgc
     1261 cttgatcgac gtgttgttgt gccgttgcat aaacatcgat tactttacgc atgtccatat
     1321 cataagcaga agtgtaatac ggaatcgtct cattggatag ataaggcgct gggtaataaa
     1381 ttttaccgat tttcttctct tggcgttctt cgatcatgcg agtgatagga tgaatacttg
     1441 cactcgtatc gttgatatat gaaattgagc catttggtgc tactgccaag cgattttgat
     1501 gatacagtcc atctttcatt actgcttgtt tcaaagcatt ccaatcttca gcagttggga
     1561 taaaaatgcc ttcaaacaat gctttgactt tttctgattt tggctggaat tctccagtta
     1621 cgtatttttc gaaataagtg ccgtcagcat aagctgattt ttcaaaatta tggaaaactt
     1681 gttttctttc acgagcgata ttgttgcttt caacaagtgt ccaataattc aacagcatga
     1741 agtaaatatt tgtaaagtct aatgaatcct ctgagccata ttccatttgt tcttttgcaa
     1801 gataagtgtg taagcccatg gcacctaatc caatcgtatg attcagctta tttccgtttt
     1861 ggatcggtgg tacgacatcg atatcagatg catctgtaat aaatgtcaat gcacgagtca
     1921 tcgtacgcac agattgacca aagttagggc tttgcatcaa attcacgata tttgtcgagc
     1981 ctaagttaca tgagatatct gttcctaata cttcatattc ctgtttgccg ttaatcacag
     2041 aaggcgtttg aacttgtaaa atctctgaac ataaattact catgatgatc ttgccatcga
     2101 ttggattctc gcggttcgct gtatcaatat tgattacgta aggatagcca gattcttgtt
     2161 gtaattttga gatttcattt tcaagatcac gcgctttgat tttttgtttg cgaatgtttg
     2221 gatttgcaac tagatgatca tactcagctg taatatttac ataagagaat gggacaccat
     2281 attctttttc gacactatat ggactaaata aatacatatc ttcgttttta cgtgtcaatt
     2341 cataaaattt atcaggtacg atcacgccaa gagaaagagt ttttacgcga atcttttcat
     2401 ctgcattttc tttttttgct gaaaggaaag caataatgtc tggatggaaa acgttcaaat
     2461 aaaccacgcc agctccttga cgctgaccta attgattaga atagctgaaa ctatcttcga
     2521 ataatttcat gactggaacg acaccacttg cagcaccctc atatcctttg ataggagcgc
     2581 cggcttcacg aagatttgat aaagtaatcc ctacgccgcc accaatacgg gacaattgca
     2641 aagcagagtt gattgagcgc ccgatagagt tcatatcgtc agtgacttga gtcaagaaac
     2701 aagacaccaa ttcgccccga cgtttacgtc cagcattcaa gaaagaaggt gtagctggct
     2761 gatatcgttg atggatcatt tcgtctgcaa gagcaagtgc taattcttca tccccgttcg
     2821 caaagtaaag cgcattgaag gctactcgat cttcataagt ttccagataa gcggaaccgt
     2881 cgttggtttt caacgcatat tgagaataaa atttgtaagc agccataaaa gatttgaaag
     2941 aaaaatgttg ctcgtccaaa aattgatata gcttttcaat aaatgatggt gaatacaatt
     3001 gaatgaattc tgcttctata tattcttgat cgatcaagta attgattttt tccataatag
     3061 atggaaaagt catcgtattt ggtttgacat tttcctcaaa aaaggcaact aaagcttctt
     3121 tatccttatt caaagggatt tgaccattga ctggccgatt gatttcatta ttcagcttga
     3181 aataactgac atcttgtaaa tttttaagac tcatacgtac gcccaacctt catttcgtat
     3241 ttgatgatgc ttgattgcaa atcattgcat tgtttttaag ctttaaaaaa ggatttggaa
     3301 gtttgcgaca aaaactggtg ctgtctcaag cccttcaaat ccatgtatca cgttttgatt
     3361 cttttagtca tgttagctgg caagtttacg caactgatca ggacggaatc cgataactgt
     3421 ttctgcatca gaagtaatga caggaacact ttggaatcct tgtgctttca accagtctac
     3481 tgcttcaggc tgttcatcga tattgacttc ttcaaaagca atttgatttt ctgttaaaaa
     3541 ccgtttgacc atcttacatt gcatacaatt atttttagaa aagattttta cgttcatggt
     3601 gagtgcctcc tctaacacat tcttattatc tataatagat agtataaaac tcttagtaaa
     3661 aaagtcaaac aacaaacact acatattgtg ttgtggaaac tgtggaatac taagttttgt
     3721 gtttttgaaa aaagagctga aaacaagtat cgtgtgggaa aatagcaaat ttaatagaga
     3781 aaaaattgtg gaaaactatg aagaaatatt tttttctcat ttttatctat caatactaaa
     3841 gattcgctca taatatcaca agtataattg acattctcaa ttaccgagag tattctatgt
     3901 gagaatgatt ctcatttatt tgcgtaacac gtaagaaaat tagaacatat aatgaatgat
     3961 tttaatacat gatttatttc acaagagagg aatcaatatg cagaccttag cgcaggcaaa
     4021 gcagaatttc gtttatcaag tcgaacgaat cgaaacagaa gaaaaaacaa aacgccattt
     4081 gcaaaatatc gggatcgttg ccggaagtaa aatcgtcgtc gtcaatttat caggcaacaa
     4141 tgccatttta ctggtaaaaa gtcatcgagt ggcagtaagt cgggaaatac taggtcaaat
     4201 ctttgtcaat aaaaagactg cttcctctga aagctggact tctcttgatc aattaaaagc
     4261 aggagagact gggatcgtcg tagcaatcca cggacaaggc gcagtgaagc gacggttaat
     4321 ggatatggga ttgacgcgaa atgtaaaaat agttatccga aaactagccc ctctgggaga
     4381 tccaatcgaa ctgacagtgc gagggtatga actgacgtta cgaaagtcag aggccgaact
     4441 aatcttgata caaagggagg aaaaagaatg aatatcgcgt tagtcggaaa tccaaacagc
     4501 gggaaaacta gcttgttcaa tctcttgact ggttcgaatc aatatgtagg gaattggccg
     4561 ggcgtaacag tagaaaaaaa agaaggatcg ttgaaaaaaa atccgaacat tcatattcag
     4621 gacctacccg gcatctattc tttgtctcct tatacaccag aagaagtgat tgcaagagat
     4681 tatttggttc aggaacagcc tgaactgatc atcaacatta ttgatgctac caatttagaa
     4741 cgaaatcttt atttaactac gcagttattg gatttagaca ttcctatgat cattggtctg
     4801 aatatgatgg atgtcatcaa aaaagaagga aaaaaaatca atctagaaaa gctttcctat
     4861 ggtcttggtg taccagtttt accaatcagc gtattgaaaa agcaaggaat cgaaaaacta
     4921 atcgaaaaga ttcaggagtc tgcaaaaaag cggccttcta ttcctgttcc ctgtacatat
     4981 gatccacgtt tagaagcagc tttacatgaa attgaagatg tactaggaga acaaagcggc
     5041 aagaaacgct ggtatgcaat gaaatatttt gaacgagatc agaaggtaag agaggattac
     5101 gagatagcag tttctcagca aaaagagatc gagcaactga tccagttaac agagaaacta
     5161 ttggacgacg atagtgagac gattctggtc aatgaacgtt atgaatttat tacccaacta
     5221 tgtacactct gtgttgtgtc aaatgattct ttccaattat cgatgagtga caaaatcgat
     5281 cagattgcaa cgaatcgttg gttagccttg ccgatcttcg catttgtgat gtggctgatc
     5341 tattatctgg ctattcaaac agtaggaaca atggggacag attggatcaa tgacacattg
     5401 tttggtacat ggttaccaga acatgttggc agattgttag ctgaatggca agtagcggga
     5461 tggatgcagg agctgatctt gaacggcatc atcgcaggcg tgggagcagt gttaggattt
     5521 ctaccgcaat tgattatatt gtttttatgc ttgagctttc tggaagactg cggatatatg
     5581 gcgaggatcg cttttgtcat ggatcgactt ttcaggaaat tcggtctttc aggaaaatcc
     5641 ttcattccta tgctgattgc tacaggatgt ggagtgccag gcgtcatggc cagtcgcacg
     5701 attgaaaacg agcaagatcg tcgtttgacg atcatggtca caaccttcat gccttgttct
     5761 gcaaagttgc cgattattgc cttagtagca ggggcctttt ttcctcatca atcttgggta
     5821 gctccttctg cctattttct tggtatgact gcaattattt tctctggaat cagcttgaaa
     5881 aaaacacgtt tgtttgctgg agatacagct ccttttatca tggaattgcc cgcatatcat
     5941 tttcctcaat ggtcagcggt cctaagacaa acctatgacc gcagcaagtc ttttgttaaa
     6001 aaagcaggaa cgattatctt tgtttctagt atcgtgatct ggttcagctc ttcttataat
     6061 ttttatgctc aacctgtttc tgaagaccaa agtatcttag cctttttcgg gcgaatcctg
     6121 gcagttgttt ttcagccgct tggttgggga aactggcaag gaacagtcgc agcgattaca
     6181 ggattggtcg ctaaagaaaa tattattggc acatttggta ttttgtttgg tcatgcggaa
     6241 gtatccgaaa atggcagaga aatctggcaa gtgctacaac acacatatac tcccgcagca
     6301 gcttattcat tgttagcctt caatttactt tgtgcgcctt gttttgcggc aattggggct
     6361 atccatcggg aaatgaatgc caagaaatgg acttggatcg ccataggcta tcaatgcggt
     6421 ttagcttatt tagttagttt tgtgatttat cagttgggac acttattgga gggtggacag
     6481 gtagcgttag ggacgtatct tgctatttta ctgatgatag gactcgttta tgttttgttc
     6541 cgtcagccaa aatccaagaa ccagttttat actatattgt cattagaagg agaggaatag
     6601 gatggcgaca ttcttattaa gtgtgttaat ctttggaagt gcaggcgcaa ttgtctatac
     6661 tcgtttaaaa tccggtaaga attgtgatga ctgtcaaaca gcttgcccag taaaaaagga
     6721 acagatcaag cgatgatcag tcgttgcaag aggctattct cccctttttt gcttattttg
     6781 ctagagcata gtaagatgag ggaaagtaaa aaaagaaaga ggaaagaaaa tgtctggaaa
     6841 gaaaaaaatc gtccgcccgc gctaccagca gattgctgtt gatttagccg aacgtatcgt
     6901 tgaaaaccga tataaagtgg gtgagaagat ccatgcccgc tcaacacttg ccagtacgta
     6961 caatgcttct cctgagacta cccgcaaggc tatcaatgtg ctggtagatc ttggaatcat
     7021 ggaagtacgg catggaagtg gggcatttgt tgcttcgaag gaaaaagcaa aagatttcct
     7081 attgcaatat caagatgtac aatccatcca agatacaaaa gcagaaatca tggcaagtgt
     7141 ggagaagcaa caagatgagc tgaatcattt ttctcaatta ttggatcagc tggtcaatca
     7201 aacgaagcga atacataaga tgaatccttt acttccgttt gaattatctt tatcagaaaa
     7261 agcaatgcat ctggaccgtt caattagcga gttgaatatc tggcaggcga ctggcgccac
     7321 agttatcgca atttctcata atgaagaact gcttgtttca cctggcccct atgctaaatt
     7381 tacggcaggg gacatcgttc attttattgg aaatgaatat acactacagc gtatgacaaa
     7441 ctttttttac ccggagcatc agcttgcaga atgattgtgt gaaatattaa aaaaatatta
     7501 acttggctaa accttttatt tacaaagatt tagccaagtt tttttcagtt tggacaagca
     7561 aatttctttc atttttgaca aagtgactac atgatgttaa catagtgtgg tcactttttt
     7621 cagaaagaaa aatgaattta cataaaaaag gagaatttgt ttggtaaaag tagaagtaaa
     7681 acatttaaca aagatatttg gtaaaaagac acaagccgct ttagatatga tgaatgatca
     7741 ccagccgaaa acggagattt taaagaaaac cggtgcaacg gttggtgtgt atgacgtgaa
     7801 ttttgacgta aaagaaggag aaatcttcgt gatcatgggg ctttccggga gtgggaaatc
     7861 cacattgatc cgtctattga atcgtctgat tgagccaact tcgggcagta tctatattga
     7921 tggagaagat gtatcgaagt taagcaaaga agaattgcga gaagtccgtc gtcataaaat
     7981 caatatggtt ttccaaaact tcggtctgtt tccacatcga acgatcttgg aaaatacaga
     8041 atacgggtta gaagtccgtg gtgtacctaa agaagaacga caagaaaaag cagaaaaagc
     8101 attagaaaat tctagtttgc tttcatttaa agaccaatat cctagtcagt tatctggtgg
     8161 gatgcaacaa cgggttggcc tagccagagc tttagcaaat gatcctgaga tcttgctgat
     8221 ggacgaagcg ttctcggctc ttgatccgct gatccggcga gaaatgcaag acgaattact
     8281 ggacctgcaa gccaatgttc aaaaaacgat tattttcatt acccatgatt tgaatgaagc
     8341 tttacggatt ggggacagaa tcgccttgat gaaagacggc gaaatcatgc agatcggaac
     8401 aggtgaagaa atattgacca atccagcaaa tgattatgta cgtgaatttg tggaagaagt
     8461 cgatcgttcc aaagtgctta cggctcaaaa catcatggta cctgcattga cgacgaatat
     8521 cgaatctgat ggaccaaacg ttgcattgac aaggatgcgt aatgaagaag tcagcatgct
     8581 gatggctgtt gacagaaaga gacacctgaa agggatcatc actgcagatc aagcgttaga
     8641 agctaggaag caaaaacgtc cattgatcga cttcctagat gaaaatgtga cggtgattgg
     8701 gaaagacatg gtggtaagtg atattttcaa tattatttat gactcaccga ctccacttgc
     8761 agttgtagag gatggaaaat taaaaggtgt cgtcatccga ggtagtgtga ttgaagcatt
     8821 agcagaaaca agcgaggtga gtgaacatga ataatctatt gaactatcaa ttaccagtgg
     8881 caaactgggt agaaaatatc acagaatggt ttacgacaac tttttctggc ctattttcat
     8941 ttttacaaac gattggacag gcagtgatgt cggggattac aaatttactg ttagtgattc
     9001 cagctccttt gtttatcttg ttattgacgg tagcagcttt ctttatttct aagaaacgtc
     9061 caggacttac gttgttcaca ttaatcggtc tatggtttat ttataaccaa ggtctatgga
     9121 atgatttgat gaatactgtc acacttgttc ttttatctag tgtgatctcg atcatcattg
     9181 gggtaccatt agggatcttg atggcaaaaa gcagtaaagc ccaaagtatt atcaaaccga
     9241 tccttgactt tatgcagaca atgccaggat tcgtttattt gattccagca gttgcatttt
     9301 tcggtatcgg gatggttcca ggggtatttg cctcagtcat ctttgctctg ccaccaaccg
     9361 ttcgttttac gaatttaggg attcgacaag ttcctaaaga attagtagaa gcttctgatt
     9421 cattcgggag cacaagtcgt caaaaactat tcaaactaga attgccttta gctaaaagta
     9481 cgatcatggc agggatcaat caaacaacaa tgctgtcact ttcaatggtc gttattgcct
     9541 ctatgatcgg tgcacccggc ttaggacgcg gcgtattatc agcattgcaa agagcacaag
     9601 tcgggaatgg atttgtcaac ggggttgcat tagttatctt agcaatcatc attgaccgtt
     9661 ttacacagca tttgaaccag ccaaacaata aaaaagctgc aggagcaaca acacagaaaa
     9721 gtaaaaaacg tcaaggactg atcattggtg ctgtagtagt agtgatctta ggtgcgattg
     9781 gtatagggag tttttcatct gccaaagaaa caaaacgtat caacttatct tatgttgaat
     9841 gggatacaga agttgcttca acaaatgtag tcggtgaagt attgaaacaa atgggctatg
     9901 atgtaacgat gacgccatta gataattcaa tcatgtggaa atctgtttca aatggcgagt
     9961 cagatgccat ggtttcagct tggctaccta aaactcatgg ttcacaatat gcacaatata
    10021 aagatcaagt cgaagaccta ggtgccaatt tgacaggagc aaaagttggt ttagcagtgc
    10081 cagcctacat ggatgtaaat tcgatcgatg agttaaccga tcaggcaggt aagaaaatca
    10141 taggaatcga gccaggagct ggtgtagtta cggctgctga aaacacgatc caaaaatatg
    10201 acaacttgaa agactggaaa gtagagacat cttcttcagg agcaatgacc gttgcactag
    10261 gtcaagcgat caaaaaacac gaaccaattg tagttacagg atggacacct cattggatgt
    10321 ttgccaaata tgatctgaaa tacttagaag atcctgaaaa cggaatggga agcgaagagc
    10381 agatccatac gatggttcga aaaggattga aagaagacca gccagaagct tacaaagtat
    10441 tagacaattt ccattggtca gaaaaagata tggaaaaagt tatgttggaa atcaacaatg
    10501 gaaaagatcc acagcaagca gcaaaagact ggatcaaaga gaaccaagaa ttagtcgaaa
    10561 gctggaaaaa ataaaaagac agacacgaaa taaatggagc ttccaaaatt ggatcaaata
    10621 tccaaatttg gaagctccat gttcgtctct atcttttttg tctataaaaa aagagaatgc
    10681 gtagctcatc ttcgaaacgt tcaaagacgc cgacaaacta ttatgaattt atgggtgcga
    10741 gtcaaagaga tcgatcgtag gtatgaaaga ctaatgtgat taaagtttat tatgtacgaa
    10801 gtgcat
//
LOCUS       DAXWOO010000092        10559 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.092, whole genome shotgun sequence.
ACCESSION   DAXWOO010000092 DAXWOO010000000
VERSION     DAXWOO010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10559
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.092"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(78..2198)
                     /locus_tag="ACGE70_001855"
     CDS             complement(78..2198)
                     /locus_tag="ACGE70_001855"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFF8693485.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQLEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHKG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2428..3606
                     /locus_tag="ACGE70_001856"
     CDS             2428..3606
                     /locus_tag="ACGE70_001856"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFF8693486.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3622..4380
                     /gene="aroD"
                     /locus_tag="ACGE70_001857"
     CDS             3622..4380
                     /gene="aroD"
                     /locus_tag="ACGE70_001857"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFF8693487.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4403..4888
                     /gene="ybaK"
                     /locus_tag="ACGE70_001858"
     CDS             4403..4888
                     /gene="ybaK"
                     /locus_tag="ACGE70_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFF8693488.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSHIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4959..6212)
                     /locus_tag="ACGE70_001859"
     CDS             complement(4959..6212)
                     /locus_tag="ACGE70_001859"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFF8693489.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6328..7677
                     /gene="gdhA"
                     /locus_tag="ACGE70_001860"
     CDS             6328..7677
                     /gene="gdhA"
                     /locus_tag="ACGE70_001860"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFF8693490.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNAQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7789..9135
                     /locus_tag="ACGE70_001861"
     CDS             7789..9135
                     /locus_tag="ACGE70_001861"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFF8693491.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9443..9829)
                     /locus_tag="ACGE70_001862"
     CDS             complement(9443..9829)
                     /locus_tag="ACGE70_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFF8693492.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYTEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(10097..10246)
                     /locus_tag="ACGE70_001863"
     CDS             complement(10097..10246)
                     /locus_tag="ACGE70_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693493.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
BASE COUNT         3380 a         1905 c         2115 g         3159 t
ORIGIN      
        1 tattctaaga tttctcttta ccaacatcat ttgacaaaga gcctaatttt gtcgctgctc
       61 cttttaaatt tattttatca ttcggaagaa cttgtggtcg ttccgtgaag ttcttggtac
      121 gtttgcgttt tataaagatc aacagttgat tgtcccttgt gttcactaaa gacactagtt
      181 gacttgtcac caagagattt ttcagcttct tttaatttct ctaagccatt cttgtagtca
      241 taatcactgg tatcaattgg gtctaatcca ctattcgtat agaaccgaag aagatcacca
      301 ttattgattt ggtcagaaac agctaactgt ttatcaactt tttctttcag tgaatcaatt
      361 tcattctgta cttcctcagt aggttcagta atagcttcac cagtttcgtt actatagatc
      421 gtaccaccgt agtaagtata ttttggtgta acaaaatcac catcacggaa agaaacaagc
      481 tgttcatgat ctttcgagaa tagatcttgt cctaactgaa tataattttt tgtatctaca
      541 cctagtaagt gaagtaatgt aggcaaggca tcaacttgtc cgccgtaagt gtgattgatt
      601 ccgccctgtg tttgtccagg gatatggatc atataaggga cacgctgcat ttgtgcattg
      661 tcgtagtttg tccaagtaga acttgttttt ccaactaatt cagctagatt tttatttctt
      721 gaattcgaga caccgtaatg gtcaccgtaa aggacgatga ctgaattatc atacaagccg
      781 ctttctttca aataattgaa gaattcttgt acggcggtat ctaaataatt ggctgtcgca
      841 aagtaaccgt tgatcgtttc atcatttgta ttggctaacg ggaatcctgc ttcgtcattg
      901 gtgaattttg aataaggata atggttagat acagcaatga attttgaata aaatggttgt
      961 tgcagacgtt ccaagtattg tacggattgt tggaaaaatg gtttatcatg caacccgtat
     1021 tggaacgaat tgtcatcatt aacatcataa taagaagaat caaagaaata atcatatcct
     1081 aaattcttgt atgtctcgtt tcgattccag aatgtaccag cgttcccatg gaataccgca
     1141 ctagtgtaac cttgtgtttg acttaaaata tcaggtgcag cttggaaagt attttttccg
     1201 cctaattgtg tgaacaagga gccttgattc aagccaaaca atgaattttc catcagcgtt
     1261 tccgcatcgc ttgtttttcc tgcttttact tgatggaaaa agttgtcaaa actgaacgtg
     1321 ctgttgctgt ggtaaagact attgatgaaa ggtgtaactt caagctggag accattctca
     1381 tctgtcaatt tataatcgat caggaattgc tggaagcttt ctaaatggat ataaatcaca
     1441 ttttttcctt tagcgagccc gaacaaatca tcatttggtg ctgcataatg ttcttttaca
     1501 taattttcta cttctttcat atcattggga cttgcttctg cccgcacttg gtttgtctgg
     1561 taagtttgaa caccgtcata aaccatgaaa gcattcaagc caagatattt gaccaaataa
     1621 tcacgagaga atgttcgagt cagcaattct ggacgatcag cttccgccat aaaaagattt
     1681 cctgagaaaa tcataactgc taatgtagag atcgcaaaag ccattcttgc tcggatcggg
     1741 cgagggtcca acttgatttt ttttgcaatc agcagaacag tcaagataat aaaatcgatc
     1801 caatagatca catcatatgg gcgaaacagt cgaatggcac tttctcccag accactcgct
     1861 actttcccag cacctaaaat cgtgttgatc gtaatgaagt ccgtgaattc tcgataataa
     1921 gtgacattcg aaaaaacaag caatgtcaaa agaaaataaa tgaccatcat cgtgatataa
     1981 gcaactctag ggcgtctgat ataaagagca attccaagta ataacaatgt agttgcaata
     2041 gggttgataa acaagataaa gtattgaatg ccactttcga ttccgagatg gaaatcaact
     2101 aaataggcaa aaatcgtttt caaccaaagt aaaacaacta gtaaagaaaa aaatccgagc
     2161 ctcttattca aaaaggcagg tgtttttata catttcaaga taatcttcct ttctgcactt
     2221 cttaatcaca gaagtgtcat gaaaccatgt tacacagctc acttattctc atttatttta
     2281 ctagtatgac aatatagtgt caattgaaac cagaagttta tgctgtttca ttaaggtttt
     2341 ctttaagctt catgaaaaaa tgagcagtaa gacaagtcct atgttttttg ttatacttgg
     2401 gaagagaaag ctaaagcagg tgaaggaatg aaagtaaaag taaaaaaaag agcaagcaat
     2461 cgattgaaaa aaggatatcc actgatccaa aaagaagacc ttgctcatcc ccaaaaagaa
     2521 accacgcaat gggtggaatt agttgattcg caaaaccaat ttatcgcaaa aggatatcta
     2581 ggaaagcaga ataagggaat cggttgggta ttgagccagc agaacatccc atttgatcaa
     2641 tctttttttg aagaattttt tgtgaagtca aaagaaaaaa gaactttctt tttttcagat
     2701 gattctacta ccgcttttcg tatattcaat ggagaaggag acgggatcgg cggtctgatt
     2761 gttgattatt atgcaggtta tgctgttttt tcttggtata acgaaacgtt atatgcacat
     2821 cgtgaattat tgattcaagc ttttcgtgaa atctacccgg aaatcgtagg aggctatgag
     2881 aaaattcgat ttgaatcagt aaatctgcct gagagccagt tcatttacgg aaaagaggca
     2941 ccggaaccct tgatcgtaaa agaaaacgga gtaaaatttg ccgtttattt aaatgaagga
     3001 ttaatgactg ggatcttttt agaccaaaaa gaagtacggg gatcattagc agaaggtttc
     3061 gcttctggga aaacagtttt gaacatgttt agttataccg gcgccttttc tgttgctgcc
     3121 gcaatgggag gggcaatcga aacaacaagt gtcgatctag caaaacgcag tttaaaaaaa
     3181 acacaggaac agtttgaagt gaatggtttg gatgtcgatc agcaaaaaat cattgttatg
     3241 gacgtattcg agtatttcaa atacgccaaa agaaaacaat ttttatatga catgattgta
     3301 ttggatccac caagttttgc cagaaataag aaaaaagtct ttcgcgtagc aaaagattat
     3361 ggggaactgg tcaaggatag tctggatatt ttagcagaag acgggatact tattgcgtct
     3421 acaaatgctg cgaatgtctc gatagaacaa ttccaaacaa tgattgaaga ggaattccat
     3481 gccaaaaatg tttcttttga acaacttgct ttatatcgtc tgccaaaaga tttccagacg
     3541 acggatacct ttttagaagg caattatctg aaagtgtttg tttatcaaat taaaaataac
     3601 cattagaagg agcgatgcag aatggtttgg gtaaagggat tgaatatagg aagcggtaga
     3661 ccaaagattt gtgcgcctct tatcggggcc gtccgagagg aagtattacg tgaagcagtt
     3721 cttgcacgtg aagcaggggc cgatttagtc gaatggcgag tcgatcatta tcgtgaagtt
     3781 ttttcttttg atgaagtagc agaaacattg gttcttatcc atgagatgct tgatggactg
     3841 ccgttattat ttacgtttcg taccttagct gaaggcggag aacaagacat atcgatccgt
     3901 cagtatcgcg acctttatga acatgtgata cacacaggat tagtagatat ggtcgatatc
     3961 gagttgttca aagtagaaag tctaggaaaa agactgatag aggaaatcaa gtctttaaac
     4021 attcctttaa ttatcagtag tcatgatttt aaagaaacac cagcagaccc tgtcttgctt
     4081 taccgattga atgttatgga acattttgga gccgatatcg gaaaactggc agtaactccc
     4141 aataatgaac gagatgtttt gcggttgatg gagttgacta gacgggcaaa tgcttttgtc
     4201 tccatgccta ttattacgat gtccatggga aatcttggta aaatttctcg tctggctggc
     4261 ggaacgacgg gttccgttat gacatttggt tccttaagca gcgattctac ttctgctcca
     4321 ggacaattac cggtcaaagc attgaagcaa attattcaat tattaaatga accgttgtag
     4381 aacaagtttg aggtgatatt ttttggcaaa gaaaaaaata gtaaaaacga atgcaatccg
     4441 tatggtcgaa caaaaaaaga ttccctatac agagcacgaa tatgagtggg acgaaagcca
     4501 cctaagtgcg tcatcggtcg ctgaacagct tccagaaagc caatctcata tatttaaaac
     4561 actggttgct gtgggcaatg tgacggggcc tcttgttgct gtgataccgg gagaagccga
     4621 attgaatcta aaaaaattag caaaagtttc agggaataaa aaagtagaaa tgctccattt
     4681 aaaagatttg gaagcaacga ccggatacat acgtggtggc tgctcgccta taggtatgaa
     4741 aaaactattt cctacttatt tagatcaaat tgcagaaagc tatgaacaaa tcattgtctc
     4801 ggctggcaga agagggctac agatggagtt agcacctcaa gatatctgtg cattaacgag
     4861 cggacaattt gcagatataa aacaataaat catacgtata aaagaaagaa ggaggccggg
     4921 atgttgtttg tccagtctcc ttctttcttt tttatacatt atgctctttg cccggcattc
     4981 attcctgcta catgccctgt agcgaaagcc gctgtgatat taaagccgcc agtataacca
     5041 ttgacatcca gtaattctcc cgcaaaatac agaccatcaa tgattttact ctccaatgtt
     5101 ttaggataaa tttcctttag agaaacccct ccgccagtta caaatgattt ctcaatagga
     5161 aaagttttgt tgattgatag cttaaactct ttgcatagtt gaacaaaatc ttgaatttgt
     5221 ttttgagagg cttgttgtcc tgtcagatga tccatttcaa gccgctcaag aaagaaaact
     5281 aacaatcgct caggcaatat accatgccaa gcattgacca agttcttttt ggttgcttta
     5341 cttttttctg tcaatatatg aataagctct tgtttggttt gtgtagggaa acaatccaga
     5401 gaaagtgtca ctggttctcc cgttttctct aattctttat tgacaaaact tgaacaacgc
     5461 agtgcagccg gaccagaaat accgaaatgg gtgaaaagta aatccatcgt atgttcagtc
     5521 aatacacgac cttttttatt taaaacgcgt aaagtgatgt cttgtaaaga aagcccttga
     5581 agtgttttct cctgaatata cggttcatcc gatatcaagg gggactctgt cgcataaaga
     5641 ggcgtcaccg tgtgacccac tcgcttcacg atcttgtacc catctccagt agaaccagta
     5701 gatggataag tccgtccacc agttgttaag ataatggcag gagcatggaa ctcttcgaat
     5761 tccgtgcgta taccgtaaat tttatgttct ttgtggatca atttctctac tcgagtagag
     5821 aatagaagcg tcacatctag ttctttcaga cgattgagca atgcttcgat aatcgttttt
     5881 gatttatttg ttacaggaaa catccgtcca tgatcctctt cttttaaatg tacgccttgt
     5941 gattcaaaga agttcatgat atcgaaatta ttccattgcg caaacgtact atataaaaac
     6001 tttccatttc caggaatatg tgcaatcaaa tcatcaacag gacgattgtt cgtaacattg
     6061 caccgaccac cacctgtcat cagtagtttt tttcctgctt ttttgttttt ttcgatcaag
     6121 agtacgcgtg cgccttgttc agctgcactg atcgctgcca tcatcccgct tgttccagcg
     6181 ccgactacga tcacatcata cagtttttcc attccctcac ctcaaggaaa gtgtagcata
     6241 tctgaagaaa gaaaacagaa aaaatgtttt gagccaaaag agaaaatgag ctaaaatgag
     6301 ggaagataag caaaggagcg tggaacgatg acaaacgcaa cagaatatgt tcaagcaatt
     6361 caagaaaagc tacatcaaaa ggatcaaggg caagaagaat ttttacaagc aatcgatgaa
     6421 ttcatgccca cggttatgtc ttttttggac actcatcccg aatatatcga aaagaatatc
     6481 ttagaacttt taacggaacc agaaagagtt attcaatttc gagtgccttg gcaagatgat
     6541 gaaggaaact ggcgagtaaa tagagggtac cgtatccaat acaactcagc tatcggccct
     6601 tataaaggtg gattgcgctt ccatcctagt gtcaatctaa gtatcttgaa atttttggct
     6661 tttgaacaga ttttcaagaa tagtttaacc ggtctgccaa tcggtggagg aaaaggcgga
     6721 agtgacttcg atcctaaagg aaaatctgat aatgagatca tgcgtttttg tcaaagtttc
     6781 atgctggaat tgtcaaaaca tatcggccca tcgattgatg ttcctgcggg agatatcggt
     6841 gtaggagcta gagaaatcgg ctatttgttc ggcgaatata aacgattgaa aaaatatgat
     6901 actggtgtgt tgacaggtaa acctttggat ttttggggaa gtaagatacg tacggaagct
     6961 actggttatg gattagtgta ttatgtcaaa catctattga acgaagaaaa agattcattt
     7021 aatcaaaaaa cagtttttgt ttccggaagc gggaatgtag ccatttatgc aatcgaaaaa
     7081 gtgcaagagc taggcgggaa agttgttaca tgttcagatt ctagcgggta catctatgat
     7141 ccagaaggaa tagatgtgaa actattgaaa gaagtcaaag aaatcaatag acaacgtttg
     7201 acggaatacg caaaccaacg tccttctgct atttatcatg ctggagaatc tgtttggaca
     7261 ttgaaagaaa aagcggatat cgctttgcca tgtgctactc aaaatgaaat agacgaaagc
     7321 ttggccgaaa tcttagtaga aaatgggatc aaaatcgtag cagaaggcgc taatatgcca
     7381 tgtacagtaa aagctgtttc tgttcttcat gaagcaggcg tgtggtattg tccaggaaaa
     7441 gctgctaatg ctggaggagt agcagtatct gctttagaaa tgagtcagaa cgcccagcga
     7501 ttgacttggg aaagagagca agtggatgag cagctggatc aaatcatgga aacgatttac
     7561 catacgtgtc gccaaacagc agccgagtac ggcgacgaaa aaaatctgct tcttggagca
     7621 aatgttgctg gatttgaaaa agttgccaaa gcaatggcag cagaaggttt agtttgaaaa
     7681 atgaaacttt cttggaaata ttgtcagcat tttgtttatt tgcttgaaaa ataataaaaa
     7741 aaaacgtaaa gtattacctg tagagtaaac ctataaggag gagaacaaat ggcacacatt
     7801 cattttgact attcaaaagt agcaccgttt gtcaacgaac aagagttagg gtacatgcaa
     7861 agccaagtaa cagcagccca caacgaatta cgggaaggta ctggcgcagg aagcgacttc
     7921 cgcggctgga tcgatttacc aacgaactat gacaaagaag aatttgcacg tatcaaagaa
     7981 gcagctaaaa aaatccaatc tgattctgaa gtattagttg ttatcggtat cggtggttct
     8041 tatttaggcg ctcgtgcagc gatcgacttc ttgaatcaca cattcttcaa tttattagat
     8101 agcaacacaa gaaaagcacc acaaatcttc tttgccggaa attcaatcag ctcaacatat
     8161 attgctgact tgatcgaagt aatcggtgat cgcgactttt cagtaaacgt tatttcaaaa
     8221 tctggaacaa ctactgaacc agcgattgcg ttccgtgtat tcaaagagct tcttgttaag
     8281 aaatatggac aagaagaagc aaacaaacgt atctacgcta caacagataa agcaaaaggt
     8341 gcagttaaag tagaagcaga tgcagaaggt tgggaaactt tcgtgattcc tgatgacatc
     8401 ggcggacgtt tctctgtatt aactgctgtt ggtttgttac caatcgctgt tagtggtgca
     8461 gatatcgatg cattgatgca aggtgccgct gatgcaagca aagcatactc aagcgacaaa
     8521 ctagaagaaa acgaagctta ccaatacgca gcaatgcgca acattcttta ccgtaaaggc
     8581 aaagtaactg agttgttgat caactatgaa ccaggaatgc aatacttctc agaatggtgg
     8641 aaacaattgt atggtgaatc tgaaggaaaa gaccaaaaag gcatctatcc ttcaagcgca
     8701 aacttctcaa ctgatttgca ctctttagga caattcatcc aagaaggacg tcgcaatatt
     8761 tttgaaacag ttgtgaaggt tgaaaaacct cgtaaatcaa tcacaattcc tgaacaagca
     8821 gaagatttag acggtctagg ctacttgcaa ggaaaagaag tggacttcgt aaatacaaaa
     8881 gcatttgaag gaacattgct tgctcataca gacggtgacg tgcctaactt gctagtcaaa
     8941 attccagaaa tggatgctta tacattgggt tatacaatgt acttctttga aattgctgta
     9001 ggtatttcag gttacttgaa tggaatcaac ccattcgacc aaccaggtgt agaagcttac
     9061 aagaaaaaca tgttcgcact tctaggcaaa ccgggctttg aagatcttgc taaagaattg
     9121 aacgaacgcc tgtaagataa gggtttttcg ctagatttaa ataatcagtg atagaaacat
     9181 gttcgcttta aaatgaaaac tggtacccat ttaggtactc aaacatactg catcgtttat
     9241 tcagaataag gggctgtgac aagacgtttg tcacagtccc ttgtttgtat agaaatcgat
     9301 tgattgcctt gctctttagt ttgttttagg actttaagaa cagtgaggct gacttcgaag
     9361 gttaattctg tttttttgtt ttctagaatc agtaagcaaa aaagcactca cttcgagtgc
     9421 ttttttgcga tatattctta gcttaagata ggcctaattt ttcttttaca gatgagggta
     9481 ccttatcgct ggtaattact tttttctcta tgatccgctt ttcgcttgct ttcaactgca
     9541 agatcgttcc ttcggtgtaa gcagaaccat tggcagagtc accagaagta atgacatcga
     9601 aatctaactg tcttttttca ccgttttcag cataagcatc tacgttgtat ttaaaacctc
     9661 ttccaattac ttccccatta tctgctttga tatcagtttc tgcaggcatt ggagctttga
     9721 ctaccgcata ataatcttta cctacataag tagactgata gtagtcccaa cctttccatc
     9781 ctataaaagc aatcaatatg ataacgatca accctaaaat ttttttcatg aaagatctcc
     9841 ttttttattt ttattatatt aaatattata cagataataa cggaatatta tcatcgaaac
     9901 agctaacaaa attgtcataa aaaacgagat ggttgatatt agaacttgtg tttttgttta
     9961 ggtctagaga taaagagaga caatttccaa atatatgtaa tgaatacaaa aagacgtttg
    10021 tcgcggatta ccgttacaaa cgtcttttat tgattttccg agatctcatc agtcgccatt
    10081 tggaatggtt cactccttat ctagattcgt ttaatgctcg gactgcttgt tcgattgtaa
    10141 taccaaacgc aacgttattt tcgttttgtg catcaatcgc cataaaacgg ttagaagttg
    10201 tatcaaaagc tacgcggtat tgcatatttg tttttgtaaa cgtcataaaa ttcatctcct
    10261 ttgtaatgta atttagttct tgaaagtttc ctttaaactt tcgaacttat accaagttta
    10321 gcatcgtacg ttttttgatg caattaaaaa gcatttatca ttttccttat ttattttttc
    10381 ttaaatggtt tagctatttt cttttaatcc gataagtagc ccaatcatca aggttacaaa
    10441 tactattgct tgaatataaa tcatatgctc acctctttat aaataactta gcaatataaa
    10501 gaaaaaaacg cacgaacaac ataggaatat aaacaaacgt ttttgtcaca acgtctaag
//
LOCUS       DAXWOO010000093        10531 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.093, whole genome shotgun sequence.
ACCESSION   DAXWOO010000093 DAXWOO010000000
VERSION     DAXWOO010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.093"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            13..636
                     /locus_tag="ACGE70_001864"
     CDS             13..636
                     /locus_tag="ACGE70_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693494.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1086..2135
                     /gene="trpX"
                     /locus_tag="ACGE70_001865"
     CDS             1086..2135
                     /gene="trpX"
                     /locus_tag="ACGE70_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFF8693495.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2132..3013
                     /locus_tag="ACGE70_001866"
     CDS             2132..3013
                     /locus_tag="ACGE70_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8693496.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3016..3786
                     /locus_tag="ACGE70_001867"
     CDS             3016..3786
                     /locus_tag="ACGE70_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8693497.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4065..4919
                     /gene="aroE"
                     /locus_tag="ACGE70_001868"
     CDS             4065..4919
                     /gene="aroE"
                     /locus_tag="ACGE70_001868"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFF8693498.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5097..6260
                     /locus_tag="ACGE70_001869"
     CDS             5097..6260
                     /locus_tag="ACGE70_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFF8693499.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6377..6787
                     /locus_tag="ACGE70_001870"
     CDS             6377..6787
                     /locus_tag="ACGE70_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFF8693500.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6961..9189
                     /gene="topB"
                     /locus_tag="ACGE70_001871"
     CDS             6961..9189
                     /gene="topB"
                     /locus_tag="ACGE70_001871"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFF8693501.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSNLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9360..9644
                     /locus_tag="ACGE70_001872"
     CDS             9360..9644
                     /locus_tag="ACGE70_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693502.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9804..10040
                     /locus_tag="ACGE70_001873"
     CDS             9804..10040
                     /locus_tag="ACGE70_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFF8693503.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10037..10435
                     /locus_tag="ACGE70_001874"
     CDS             10037..10435
                     /locus_tag="ACGE70_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFF8693504.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3665 a         1770 c         2226 g         2870 t
ORIGIN      
        1 gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca agaaatgaat
       61 gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg tcttgttttt
      121 ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga aaaaatccga
      181 gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt gaaacctttt
      241 gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt gatgttcagc
      301 aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc tgagcttatg
      361 gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca tgaactggaa
      421 caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg aggaaaagaa
      481 gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga gttgatccct
      541 gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt ggatagaacg
      601 ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat gaaacggtgg
      661 gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc aaaataacaa
      721 ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact taaatcaatt
      781 cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag aaaacaaaaa
      841 aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc atagatacaa
      901 ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt gactgccgca
      961 tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc cattgtcttt
     1021 ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt tgcaggagga
     1081 aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt tatttagcag
     1141 gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg aaccagacaa
     1201 aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta gatgagattt
     1261 atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa aatcttgaga
     1321 tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt caacagctag
     1381 tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa tcattagcaa
     1441 acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg ggggctggat
     1501 tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat cagccgcctg
     1561 ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa gtcggaatct
     1621 tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa gaagctgcaa
     1681 aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag attgcacaaa
     1741 cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat aataccattg
     1801 ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc gtcattccat
     1861 ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat caatatcaat
     1921 tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa aaaccggaaa
     1981 cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct caagctgatc
     2041 gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt acagatgact
     2101 cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt cacaaggcat
     2161 gctttgggca atattaggat tagggatttt catgacttat cggatcttag actttcctga
     2221 tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag ctattactca
     2281 tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga tagcaggact
     2341 cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag ggattttagt
     2401 gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc tttctttatt
     2461 gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt actttgacac
     2521 agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat tcttcttcaa
     2581 tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg ctcggtcgtt
     2641 agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg gtgtcattgc
     2701 attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata aagggattgg
     2761 cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc aagaattgac
     2821 gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc tattgatttt
     2881 agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg ctatcatctt
     2941 agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa cgggttttga
     3001 gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa agtcgtaaac
     3061 aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat cagagaaggg
     3121 gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt caatacgatc
     3181 gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct gatcacaaaa
     3241 gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc caagatggga
     3301 acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag aggtcaaaaa
     3361 cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag actatgtcaa
     3421 gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt atctggtggt
     3481 caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt actattatta
     3541 gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg attaacaaat
     3601 cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat ggaagacgcc
     3661 ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca agatatttct
     3721 gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga agaagtagtg
     3781 gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca ttgacatctt
     3841 cctttgattc atcctatact taaaacaata aacaattaaa catatttggg tgttttgtag
     3901 gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg acaagtaaac
     3961 gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc gtatcaaacg
     4021 ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt tctggacaaa
     4081 ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca ttgatgcaca
     4141 atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa gtaaatccaa
     4201 tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt gtaaatcttt
     4261 cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag gaagcgcaat
     4321 tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc tataataccg
     4381 atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa aagaaaaaaa
     4441 tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagct gcgttagacg
     4501 gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa gtaaaagaaa
     4561 cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac tttgccgata
     4621 cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca acagatatcg
     4681 ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg catgagaatc
     4741 tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa gaagcaaaaa
     4801 aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga gcaatcgcgt
     4861 ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg tttgattaaa
     4921 aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa aagtaatcct
     4981 ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa tgataggctt
     5041 ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga accacaatga
     5101 gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag cgggatacgg
     5161 ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag gaagatgcta
     5221 cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac gagcaacaga
     5281 tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaaaaattt ttcaatcggc
     5341 aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa atcattctat
     5401 cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt aaatatgtag
     5461 ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg caaaaagcga
     5521 cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa gatgccgggc
     5581 gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg aaactgattt
     5641 cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag atcaattcag
     5701 cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat tttggaatcg
     5761 attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc gatgccttat
     5821 tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt gtagatatta
     5881 aaaaaggggt ccagaagatg gatggacatg ttcttttgca gtacgcacgt ttccgaatgg
     5941 atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac gctattttca
     6001 gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt aaagctttag
     6061 gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt tcgattgcac
     6121 gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg aattatgggc
     6181 aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa tctgctatta
     6241 cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca aggcttttgt
     6301 catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga tttaaccaag
     6361 agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc gtgaagccat
     6421 cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat acggtgaaat
     6481 gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca cgaatgcacg
     6541 gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa ttggtcctga
     6601 attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag aagaaacgaa
     6661 gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg atgctgttcc
     6721 gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc agattaacca
     6781 agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg gatcatcgtt
     6841 tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt tattgccaga
     6901 tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg aggtcagaag
     6961 atgaagacgg tgatactagc tgaaaaacct tcacaagcaa aagcctatgc cgaaaccttc
     7021 caacagcaaa agcggcatga agggtattat gaaatcagcg atccattgtt tgctggtgaa
     7081 gtaaccatca cttatggttt tggacatttg gtagatatgg ttgctccagg agcttatgaa
     7141 gagcgttggg caaaatggtc tttagaaaat ttgcctattt tcccagaaac cttccgttac
     7201 gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa aacgagaatt gcagtcagcc
     7261 gataccatca ttattgcaac agatggtgat cgagaaggtg aagccatcgc gtggtcgatt
     7321 attcgacaag caaacgcatt ttctaaggaa aagaactatt tacgtttatg gatcaattct
     7381 cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc aacctggaaa gaactatttt
     7441 ccaaaatata aagaagcgca agcaaggcag aacgctgatt ggctgattgg aatgaatgga
     7501 agtccattat atagcttgct acttcaacga aaagggattg atggcagttt ttctcttggt
     7561 cgtgtacaaa caccgacact ctatatgatt tatcagctgc aagaagaaat caaacacttc
     7621 aaaaaagaaa cgtactttga aggagaaggt ttaattacga caaacaaagg gcagttttct
     7681 ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag agttaacgaa ggaaatagaa
     7741 aaacttggag cgcatttagg taagcaaaac ggacggattc ttgaagttac gaaaaaggaa
     7801 aagaggttgc atagtccacg tttattttcg ctatctaatt tgcagacaaa gatgaatcag
     7861 ctaatgaaag caagtgccaa agctacactt gatgctgctc aaggattgta cgaaagcaag
     7921 tttctaagct atccaagaac ggattccttt tacattacag aaaatgagca tcagtatctg
     7981 gttaaacatc tgcaaaatta taaaaaattt ttaggaatag aagaagtaca gacaactgag
     8041 atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc aagaacatca tgcaattatt
     8101 ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc tttccaaatt acagcaagca
     8161 atctatttgc aagttttgcg cactactgtg gctatgtttg ctgcagatta tatctacgaa
     8221 gagacgaccg taatgacggg cgtacagcaa ttaaagctaa aaagcatcgg aaagatccca
     8281 ttacagcaag gttggcagga aatcttacgc atcaagtcaa aaaagaaaga agctcagact
     8341 cttcctccta tcgtagaaaa tgaagaagca gaagtagaac tgaaaagtat ggagaaagaa
     8401 acacaacctc caaaacccta taccgaagga acgttgatca cagcaatgaa aactgcggga
     8461 aaaacgctgg ataatgatga agcgcaagaa attctcaaag aagtagaagg aatagggaca
     8521 gaagctacac gagccaatat cattgaaaat cttaagcaga agcattacat cgaagtcaat
     8581 aaaaacgaaa taactgttac gccaaaagga atcactcttt gtaaggcagt agccaatgaa
     8641 ccattactga cgagcgcaga aatgactgcg cgctgggaag ggtacttgag aaaaattggt
     8701 aaacaagagg ggacaccatc tgttttttta gagaatatca aaaaattcat cctacattta
     8761 ttggcagaag ttcctaatca aatcgacaag gtagactttt cagatgagat tagcgggaca
     8821 cgtcagataa aagcgatgga gaagaaaaac gatcaattag gaagatgtcc aaaatgtaaa
     8881 aagggaatag tcatgcttta tcctaaaatc gctacatgct tgaatccgga atgcgacttc
     8941 aaattgtggc caacagtagc taaaaagaag ctgactaaaa cgaatctcag agatttgctt
     9001 tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg gtaaaaaagg gaaattcgat
     9061 gcagttttag tattaaaaga agatatgaca atcggttttt cttttccatg gatcgaaaac
     9121 agtggaacaa aagcagttga tggaaacaaa gaaacatcaa gaaaaaataa tgaaaacgaa
     9181 gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt ccgaaaagtg agaatataaa
     9241 taccaataaa actaaaaaat atcagtgaat caagcaaaaa cgttggggta ggctgatttt
     9301 atatagatag caaggagttt ttttatttga aaaaagtgca ttcattttta aattatctta
     9361 tgggagcaat cacgattgtg gctgtgtacc aagcatttct aaagtatcct gattacggga
     9421 cgaagttgtt gtcattgacg cacaatcaaa cagcatattc accaacgttg attttatcga
     9481 ctttgactgc aggagctaaa agcgctactt atttttttca taaaacgaca agcatgctaa
     9541 agcgaaagaa acagcgaaaa caattgttgg tggtggtagc ggctatgatc attatttatt
     9601 ttctaccagt tattacacaa gtatttcatc aatattttgg ttaagaatac gagagtttgt
     9661 gacaaaagtc atgtcacaaa cttttttctt tgtcattttt tcgtaagaaa aagcaaaata
     9721 aaagtatata cattgattaa aagtatatac ttggatataa tgtacttact atagaatgag
     9781 aatggtatag agggtggcga aatatgaaaa aaatgaaaaa gtataaaatt agaaaatctg
     9841 gcaatagcga tgtaacaact atcccaccag aagtaaaaga attcatgggt gtgcagacag
     9901 gtgacgcaat cagttatgtt tttcaatctg atggttctgt acgaatgatc aaagcacaag
     9961 aagaacctga tattgattca ttagttgatt cgatcatgaa tcaatatgaa gacgcgttaa
    10021 aagatttggt tgatttatga ggtatctttc gactaaagaa attatcaaaa tcaacgcgaa
    10081 agttatttta cgtcactcgc caggcgaaat gatcggtgta aaagatgcaa atgcattaga
    10141 tatggccgtc aaacagcctt cacaagcagt attcaatcaa gaactctatc ctgaaatcta
    10201 tgaaaaagcg gcgattttag caattaattt agctaaaaaa catccttttc ataatggcaa
    10261 taaacgaact gctcttgttg ccatgttgtt gtttcttcaa ttgaataatt gtccagttgc
    10321 tttttctcgt caagaagctg ttgattttat cataatgatt accactagtt cattggattt
    10381 cgattcacta aagtccaaga taaccagtta tttacgacaa accgcaataa aatgaacgaa
    10441 ttaaaaactt tatttctcaa ttagatcagc atgtcattaa ggaatatgct gattttttta
    10501 tgtttaatca tacaaaatag ttttttcata a
//
LOCUS       DAXWOO010000094        10496 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.094, whole genome shotgun sequence.
ACCESSION   DAXWOO010000094 DAXWOO010000000
VERSION     DAXWOO010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.094"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(1..461)
                     /locus_tag="ACGE70_001875"
                     /pseudo
     CDS             complement(1..461)
                     /locus_tag="ACGE70_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
     gene            complement(713..1072)
                     /gene="rplT"
                     /locus_tag="ACGE70_001876"
     CDS             complement(713..1072)
                     /gene="rplT"
                     /locus_tag="ACGE70_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFF8693505.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(1125..1325)
                     /gene="rpmI"
                     /locus_tag="ACGE70_001877"
     CDS             complement(1125..1325)
                     /gene="rpmI"
                     /locus_tag="ACGE70_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFF8693506.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(1397..1921)
                     /gene="infC"
                     /locus_tag="ACGE70_001878"
     CDS             complement(1397..1921)
                     /gene="infC"
                     /locus_tag="ACGE70_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFF8693507.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(1965..2090)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            2241..2873
                     /locus_tag="ACGE70_001879"
     CDS             2241..2873
                     /locus_tag="ACGE70_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFF8693508.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGNQSYLDAKN
                     MVLRESHIQKLTMQKTGLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTTIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2875..3780)
                     /locus_tag="ACGE70_001880"
     CDS             complement(2875..3780)
                     /locus_tag="ACGE70_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFF8693509.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWEIALIISLVLSIWMLIALWRRIR"
     gene            complement(3865..4524)
                     /locus_tag="ACGE70_001881"
     CDS             complement(3865..4524)
                     /locus_tag="ACGE70_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFF8693510.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     CVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVS
                     I"
     gene            4840..5784
                     /gene="mvk"
                     /locus_tag="ACGE70_001882"
     CDS             4840..5784
                     /gene="mvk"
                     /locus_tag="ACGE70_001882"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFF8693511.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5777..6754
                     /gene="mvaD"
                     /locus_tag="ACGE70_001883"
     CDS             5777..6754
                     /gene="mvaD"
                     /locus_tag="ACGE70_001883"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFF8693512.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKKGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6767..7852
                     /locus_tag="ACGE70_001884"
     CDS             6767..7852
                     /locus_tag="ACGE70_001884"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFF8693513.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7849..8913
                     /gene="fni"
                     /locus_tag="ACGE70_001885"
     CDS             7849..8913
                     /gene="fni"
                     /locus_tag="ACGE70_001885"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFF8693514.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKDPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAQEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(9162..9965)
                     /locus_tag="ACGE70_001886"
     CDS             complement(9162..9965)
                     /locus_tag="ACGE70_001886"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFF8693515.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIRDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9985..10308)
                     /locus_tag="ACGE70_001887"
                     /pseudo
     CDS             complement(9985..10308)
                     /locus_tag="ACGE70_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
     gene            complement(10341..10478)
                     /locus_tag="ACGE70_001888"
                     /pseudo
     CDS             complement(10341..10478)
                     /locus_tag="ACGE70_001888"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
BASE COUNT         3106 a         2220 c         2008 g         3162 t
ORIGIN      
        1 aaatacctaa tgacatcaag atcggcaaca caataactgc aatagaaatc actggtccga
       61 ttcctgtcat cgacatgaat ataacagcgg ttacaataca tagtaagctc atcgtgattc
      121 ttggcttgtc ccctccaagt tcaactacct ttcgaatcag ggtagctgcg attccgctat
      181 ctactagaac acgcccgaaa aaggcaccaa agaagacatt taccaagatc gatttggcgt
      241 actcagctgg ccccgtcgta tagatatgcg tcaacacatc aattactcct tgcccttcca
      301 tcgccgagtt tggtgaaaac gtgtttccga ctagcgctaa tcccatccat acagtagcca
      361 tgatgacaaa agccgaccat caagttgtag ccttttacac agtaccagac catgaaaaag
      421 aatgagataa ctaacaaaat accaataata atatcactca tgttattcct ccgtccattt
      481 tgtttttctg ataagcaaac taatatatca gtcaaactca ttctaactta tgtagttagc
      541 gctttcatta aaggatgacc ataaatgttc agttttcaat taaaaaacct taattatgtt
      601 attttagact tatacgttat tgtcctgttt catttgatgg aggattgtaa ataaaaaaag
      661 aggaaagaac ataacttttc gttatcttct ttcctcttta taattgttta gcttatttag
      721 ccaaagcttc ttttgcttgg tcagccaatg cagtaaatgc tgctgcatcg ttaacagcca
      781 agtcagctaa cattttacgg ttgatgtcga tttcagcaac tttcaaaccg tgcattaatt
      841 ttgagtagct caagccattc atacgagctg ctgcgttgat acgagcaatc cacaatttac
      901 ggaagtcgcg tttcttttga cgacgatcgc gatatgcgta gttgtatgaa ttcattactt
      961 gttcttttgc tgttttaaat aaagtgtgtt ttgaaccgta gtaacctttt gctaatttta
     1021 gcactctttt acgacgttta cgagttactg ttccaccttt aacacgtgcc atgtataatt
     1081 cctcctaata aaaataaatc ttcagattct gttatttgta cttcttattt catttttgct
     1141 agttgttggc gaatacgttt gaagtcgcca ctagaaacca tgcttgcttt acgcaattga
     1201 cggcgttgtt tttttgtttt gccgtggaaa cggtgacttg taaacgcgcg gaagcgtttc
     1261 aatccgcctt taccagtacg tttgaaacgt tttgctgatc cgcggtgtgt tttttgtttt
     1321 ggcatgacta tatttcctcc tcaaaatctt tcgttatccg actatcggct gtccaacagc
     1381 cagttagtca ttcaaattac ttactgtcgt ttttcggtgc cagcgtcaag aacatgctgc
     1441 gtccgtccat tttcgctttt tgttccactg ttgcaatatc cgcagtttct tcagctaagc
     1501 gattcaagac tttttgacca atttctttat gggtaatggc acggcctttg aaacggatag
     1561 aagctttcac tttatcgccc ttttccaaga acttacgtgc attacgaagt tttgtattga
     1621 agtcattcaa gtcgatcgta ggacttaagc gaacttcttt tacattgatc actttttgtt
     1681 ttttacgcgc ttcgcggtct ttcttttgtt gttcgaaacg gtatttaccg tagtccatga
     1741 ttcgcgcaac tggtggtttc gcaccagggg caactaacac aagatccaag tttgcttgtt
     1801 ctgcaatatt caatgcttct gcttttgtct taactcctaa ttgctcacca tctgaaccga
     1861 tcaatcgtaa ctctcgtgca cgaatgccgt cgttaaccat catatccttt gctatggtca
     1921 ttcacctcca aatattatga gagaaagaaa agcttaccga ataaacggca ataaaaaaac
     1981 gagttctctt acagaacccg cgcgtacttc acctaataga accagtgaaa actatctagc
     2041 ccagtgacat tatcaactaa ggcgagaagc gggtgcttct gcttttattt ctcaaccatt
     2101 atagtatagc aaaaactaat ctttttgtca atgtttttca tacagtttgt cttccttttc
     2161 tgaaaatgat tagattagca ggctttctag cagtgattcc cgcttctttg ctatactaaa
     2221 gaaaaacgag gtgaacccac atgaaaaaaa tcgaacctat tccaccttct cttcctcttc
     2281 ttacaaaggg agagttttat ttagaagatg aggagatctt ctctggtctt ctgatcaacg
     2341 gaggtaacca aagctatctc gatgccaaaa atatggtatt aagagagagt catatccaaa
     2401 aacttacgat gcaaaaaaca ggtttacgtc attttgaatg cagtaatgtc atttttgaaa
     2461 aatgtgattt ttccaatctg gagtggattg gtggcagttt ccaccaagtc gtgtttcatc
     2521 aatgtaaatt aacaggtacg aattttgccg aaagttatct tcgagactgt actttcactg
     2581 attgtattgc ttcgatggct tcctttagta gtacaaactt gaaagctgtc tccttcgatc
     2641 attgtcaatt agaagacagc gaattttatg aggtcacttg gaaaaacctt tttttgtctg
     2701 acaatcagtt aaccggttcg aactggtttc gaacttctct gaagggatta gactttacga
     2761 ctaatttctt cacaacaatc gctttatctc aagattcttt ggcgggacta atcgtcaatc
     2821 aagagcaagc acttgtgatc gcccaagccc ttggtttgca gatcaaagac tagatcaacg
     2881 aatccgccgc cacagtgcaa taagcatcca aatagataag accaaactga tgatcaaggc
     2941 gatttcccat ccgaaaacgg aatgggtaaa gggtaactgg agattcattc cgaagaatcc
     3001 tgtcacaata gttggaacgg tcaagatcag cgaccagacc gtcaaaaatt tcatcgtgtc
     3061 attcaagttg ttattcagca aattattata agtgccagag agctggtcta aaatctgcga
     3121 agctaagttc gtcatttcaa ctgcttgtcg tgcttcgatc agtgcatcgt ccaactcttc
     3181 tttttccttt tctgacaact tgcgataaat agccagtgcc ttgatttgtt ctaaaagtac
     3241 tacattttgt tttgttcctg taactaagta aaccaaaccg atctctaaat ctgaaagttg
     3301 cagcagattc ttgttcgttg ttttttctct taatttcaga ttaagccgct gacgttcact
     3361 attcacttcc tcgattaaag gaaaaaagga atcagaaatc aaaaacaatg tacgaaacaa
     3421 taaactgtat acactcatgt ctggcatccg ttccaaaagc gatcgaatta gtcgaatcac
     3481 gtactcggta ttcggcgtgg taaatgtaaa caaaccgtct tcttttaaaa taaaagcgat
     3541 aggactagtt tcataatgat tatcgacttt ttgctggtgc ggaacatttg atacaagcag
     3601 taacgcttca tcaaaagaat cgtactctac gcgcgcccgt tcatttttat ctagagaata
     3661 agtcagcatc tcgtcactaa tgccatattc ttcttttaag tttttaacag ctagcacatc
     3721 gttttgctgt acattgatcc aaaaattacg gtcttgatta aaatagtgtt tttcccgcat
     3781 actctatcct cctgacattt gtttccagta taaagaaaaa agtcgctcaa ataaaggttt
     3841 accctttatt tgagcaactg taaatcaaat ggatactttg gtttggcgaa tctctgctaa
     3901 tttttcttcg atcattttca agacttcttc ttttgcttga ggatcttcca caaaattata
     3961 gcggtctcca tcgatttgga tttttggact ttctttgtat tcttcatacc attgatcata
     4021 acgtctgttc aattctttgt aataatcgta tagacttggg tcaaaggaca attgttcata
     4081 cgaacgacca cgtttttcga tacgctctaa catcgtatca aaagatacac gaatatgaac
     4141 taataaatcc ggatgttttt tatgggcagc atagggaagt tcttccatca tgttttccag
     4201 cagttcatca tacacacaaa cttctgtttc tgttgctctt cctagatctg catttagatg
     4261 gaaaagcagt gaatcttcat aaatcgaacg gtccaataca ttattgtcat ctttcattgc
     4321 ttgtttgatg cttgcaaaac gtttattcaa aaaataaatt tgcagcaaaa aagcatactg
     4381 ttcagggttt gcataaaata agggcaagac ttcattatca tcaatcgatt cataaaatgc
     4441 ctgactatca aagtgttcag ccatcatttc tgttaaactt gattttccgg caccaattgt
     4501 acctgctaaa acgatcacgc tcatttacct ccagctcact tcatcactaa atattgtgtt
     4561 taactccaac cttttttaaa tataccccca atatctagta gtgtcaattg tattttttct
     4621 agtcaaagaa gaaaaaaagt caaaaaattc ttaagaaaaa agtgtttttt ctttagaaaa
     4681 cgtattctta gttttacttc tgagatggtt ttagtaaaat aaaggaggaa tgttttttga
     4741 gacccttagt ggacaaaaaa attcgtgaag cagcgcaact cgttttgggc gctttatttt
     4801 ttgcttaaaa atcactttta cgtgagaagg agttagatta tggcaaacta tggccaagga
     4861 gaatcaagcg gaaagatcat attgatgggc gagcacgcgg ttgtttatgg agaaccagcg
     4921 attgcctttc ctttctatgc aacaaaagtc accgcattcc ttgaagagct ggatgcaatg
     4981 gacgatcaac tggtttcttc ctactattca ggaaatttag ccgaagctcc tcatgcatta
     5041 aaaaatatca aaaaattatt cattcactta aaaaaacagc atgacatcca aaaaaacttg
     5101 caactgacca ttgaaagcac gattcctgct gaacgtggaa tgggatcaag cgctgcagtc
     5161 gccacagcag tcactcgtgc tttttatgat tacttagcat ttcctttgtc tcgtgaaata
     5221 ctattagaaa atgtccagct ttcggaaaaa atcgcccacg gtaatcctag tggaatcgat
     5281 gcagccgcta ctagcagctt gcagccgatt tattttacaa aagggcatcc ttttgactac
     5341 ttttctttga acatcgatgc ttttttgatt gtcgctgata caggaatcaa aggacaaaca
     5401 agagaagccg tcaaagatgt cgctcacctc tttgaaaccc agcctcatga aactggacaa
     5461 atgatccaaa aattaggata cttgacgaag caagcaaaac aagcaatcat tgagaattca
     5521 ccagaaacgt tggcacagac aatggatgaa tcacagtcac ttctggaaaa gctgacaatc
     5581 agcaatgatt ttcttaatct attgatccaa acagcaaaag ataccggggc attgggtgcc
     5641 aaattaactg gcggtggacg cggtggctgt atgattgcat tagcacaaac aaaaacaaaa
     5701 gcccaagaaa tcagtcaagc acttgaagat gcgggtgctg ctgaaacttg gatacaagga
     5761 ttaggagtac atacctatgt ttaaaggcaa agcacgcgca tataccaaca ttgctctaat
     5821 caaatattgg ggtaagaaaa atgaagaact catccttcca atgaacaaca gcctttcgtt
     5881 aacattggat gcattttata cagagacaga agtcatcttt tctgatagct atatggtaga
     5941 tgaattttat ctagatggca ccttacaaga cgaaaaagca acaaaaaaag tcagtcagtt
     6001 tcttgacctt tttcgtaaag aagccggcct ctcactaaaa gcttcagtaa tcagtcagaa
     6061 tttcgttcct accgcagctg gattagcctc ctctgccagc gggctagctg ctttagcagg
     6121 agcttgcaat actgctctta agcttggatt agacgatctc tctctttcaa gatttgctcg
     6181 acgcgggtct ggttcagctt gccgaagtat tttcggtggt ttcgttgaat gggaaaaagg
     6241 ccatgacgac ttaagttctt acgctaagcc agtcccttcc gattctttcg aagacgattt
     6301 agcaatggtt ttcgttttga tcaacgacca gaaaaaagaa gtgtccagca gaaatgggat
     6361 gcgtcgaaca gtcgaaacat ccagttttta tcaaggctgg ttagattccg ttgaagggga
     6421 tctatatcaa ttgaaacaag caatcaaaac aaaagatttc caacttctcg gagaaacgat
     6481 ggaaagaaac ggactaaaaa tgcatggaac gactttagct gcccagcctc cattcactta
     6541 ctggtctcct gacagcttaa aagcgatgga tgcggtgcgg caattgagaa aaaaaggcat
     6601 tccatgctac tttacgatgg atgctggccc taatgtcaaa gttttagtgg aaaacagcca
     6661 tttatctgag gtacaagaaa catttaccaa attatttagc aaagaacaag taattactgc
     6721 ccatgctggt ccaggaattg cgattattga ataaagggag atttttatga ttgaagtatc
     6781 tgcaccaggc aaactctata ttgccggaga atatgcagtt gttgaaacag gacatccagc
     6841 agttatcgct gcagtcgatc aattcgtaac agtcactgta gaatccgcac gaaaagtcgg
     6901 aagtatccaa tctgcacaat atagtgggat gcctgtacgt tggacaagac gcaacggaga
     6961 attagtttta gatattcggg aaaatccttt tcattatatc cttgctgcta ttcgtttgac
     7021 tgaaaagtat gcacaagaaa aaaacattct tttatccttt tatgatctga aagtaacaag
     7081 tgagttagac agttcaaatg gccggaaata tggattgggt tctagcggtg ccgtgaccgt
     7141 tgcaacggtc aaagccttga atgtttttta cgcgttgaat ttatctcaat tggagatttt
     7201 caagattgca gcactagcca atttagcagt tcaagataat ggttcctgcg gcgacatcgc
     7261 tgctagctgt tatggtggct ggatcgcttt ctcaaccttc gaccatcctt ggctccaaga
     7321 acaagaaact cagcattcta tcagtgagtt acttgccctg gattggccag gtctatccat
     7381 tgagccattg attgctcctg aagatttacg tttattgatt ggttggacgg gtagccctgc
     7441 ctctacttct gatttggtcg atcaagttca ccgttcgaga gaagacaaaa tggtggctta
     7501 tcagcttttc ttaaaaaaca gtacagaatg tgtcaacgaa atgatcaaag ggtttaaaga
     7561 aaataatgta acgttgatcc aacagatgat tcgaaaaaac cgacaattac tgcatgattt
     7621 atctgcaatc actggggtcg tcatcgaaac gcctgctttg aacaaattgt gtaatttagc
     7681 tgaacagtat gaaggagccg caaaatcttc tggtgcaggt ggtggcgatt gcggaatcgt
     7741 aattgttgac cagaaatctg gcatccttcc tttaatgagt gcatgggaaa aagcagaaat
     7801 cactccactg ccgttacatg tctatagcga tcaaagaaag gaaaaccgat gaatcgaaaa
     7861 gacgaacatg tttcactagc taaagccttt catgataaac aaaaaaacga atttgacttc
     7921 gtacggatca tccataaccc tttgccgcaa atagcagttt ctgatgtcga tctaagtaca
     7981 caggcagttg ggtttacact aagcagccca ttttatatca atgcgatgac tggcggaagc
     8041 gaaaaaacaa aaaaaatcaa ccaagatctt gcaatcgtcg caagagaagc agatttgatg
     8101 attgccactg gttcagtcag cgctgccttg aaagaccctt ctttagccga tacttatacg
     8161 attatgcgac aagaatatcc tcacgggaaa atcattgcca acatcggtgc agggacttct
     8221 gtcgaaagag cccaagaagc tatccgattg tttcacgcag atgccttaca gattcatttg
     8281 aatgcacctc aagagttggt tatgcctgag ggagaccgtg attttaccaa ttggaaagta
     8341 cttattcaag agactcaaac agccattgac gtccccctca tcgtaaaaga agtcggtttt
     8401 ggcatgacaa gagaaacgct caatgatcta gctgctttag gagttcatac agttgatatc
     8461 agcggtcgaa gcggaacaag cttcacccag atcgaaaacg cccgtcgctc caagcgggaa
     8521 ctgagttatt tagctgactg ggggcaatcg acagtctctt cattgcttga agcaaatgaa
     8581 gcagacactt ctatggaaat cctagcctcc ggaggtatcc gcaatgccta tgacatattt
     8641 aaagcccttt gtctcggcgc aaatgcagtg ggtacttcag gaaccgtact gacgcatctg
     8701 atgaatcacg gcgtagaaga aactattata ctgatgaagc aatggcaaga agagcttcgt
     8761 ctgctttata ctatggtcgg cgcaacgaat accgcggccc tacaccaaca atcactgatc
     8821 ttttccggac cagtgaagga ttggtgcgaa gcaagaggga tcgacttagt gaagtatgga
     8881 agacgcacag aaaagagcgt gggacaaaag taaagaacac ttttatccca cgctctaaac
     8941 acgtataaac ggcggaagca aaagcaactc cttcggaaat aagctgaaat tcacaaaaat
     9001 ttgaaaagca attttcgtga attccttctt atttcttgga gttaaacgct tctgtcccag
     9061 cctctttttt cgaataaatg atgtttaatc agctgtcctt cggtattcgt tcaagccgct
     9121 gatttcacaa cctctccact aaacggtgta ctaaagctct tttaaggttg ttttccgata
     9181 taggcaagta tgcctccgtc cacatacaag acatggccgt taacgaaatc agatgcgcta
     9241 gaagccaaga agacagatgg tccagccaaa tcgattggct cgccccagcg tgctgctggc
     9301 gtacgtccaa cgataaattg atcgaatgga tgacgttcac cgttttcttg tgtttcgcgt
     9361 aatggagcag tttgcggtgt agcaatataa cctggtccaa ttccattaca ttggatattg
     9421 tattgaccgt attcagaagc gatgtttttc gtcagcattt ttaatccgcc ttttgccgca
     9481 gcataagcac tgaccgtttc gcgtcctaat tcactcatca tggagcagat attgatgatt
     9541 ttaccgccgc ctttttcgat catatcccga ataactgctt ttgaaacgat gaatggggca
     9601 tttaaatcca cgtcgattac ttggcggaag tctgccgcat ccatttcgat catcggtgtc
     9661 cgtttgatga tccctgcatt gttgactaga atatcaactg aacctacatc ttctttgatc
     9721 tgttgaacca tcgcttgaac cgcttcttca tcggtcacat cgcagacata accttttgca
     9781 tcgatgcctg cttctttata atgcgctata ccttcatcaa ctgattcttg attcaagtta
     9841 ttaaatacga tttttgctcc tgctgtagct aaagagcgtg caatctcaaa tccaatacca
     9901 tagacagcac cggtgactaa tgctactttt ccatctaaac gaaacatatc catactaaat
     9961 tccatcattt tctctccctt atgcttattt taattgatcc atttgcacca tatccatgtc
    10021 tgtataggtg atattttctc cgcacattgc ccagatgaat gagtaattgc ttgtacctac
    10081 acctgaatgg atcgaccagc ttggcgaaat aatggcttgt tcattgtcta cgactaaatg
    10141 ttttgtttca tctggtttgc ccatcatatg gaacacacgt gtatcttcat tggcataatc
    10201 aaaatagaca taagcttcca tacgacgttc atgtgtatga caaggcatcg tattccaagc
    10261 acttcctggt tctaaaatcg tataacccat ttataaatga cagctatcgc tgaagcttct
    10321 actgctgtaa atatccccgt caagatacca ccgataatga tgacaatcaa tagaaggcta
    10381 ggaatcgcct cttttattat ttttccagca tgagccatct gtcctttttc gacaacaggg
    10441 tatttattct ttttagcaac gatgaatgct actagcatga ttcctagcgc ccacaa
//
LOCUS       DAXWOO010000095        10495 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.095, whole genome shotgun sequence.
ACCESSION   DAXWOO010000095 DAXWOO010000000
VERSION     DAXWOO010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.095"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            105..1001
                     /locus_tag="ACGE70_001889"
     CDS             105..1001
                     /locus_tag="ACGE70_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFF8693516.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1151..2092)
                     /locus_tag="ACGE70_001890"
     CDS             complement(1151..2092)
                     /locus_tag="ACGE70_001890"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFF8693517.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2218..3339
                     /locus_tag="ACGE70_001891"
     CDS             2218..3339
                     /locus_tag="ACGE70_001891"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFF8693518.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3597..5099
                     /gene="eat(A)"
                     /locus_tag="ACGE70_001892"
     CDS             3597..5099
                     /gene="eat(A)"
                     /locus_tag="ACGE70_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFF8693519.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5166..5693
                     /locus_tag="ACGE70_001893"
     CDS             5166..5693
                     /locus_tag="ACGE70_001893"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFF8693520.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            5904..6965
                     /locus_tag="ACGE70_001894"
     CDS             5904..6965
                     /locus_tag="ACGE70_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFF8693521.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            6968..7864
                     /gene="murQ"
                     /locus_tag="ACGE70_001895"
     CDS             6968..7864
                     /gene="murQ"
                     /locus_tag="ACGE70_001895"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFF8693522.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            7878..9860
                     /locus_tag="ACGE70_001896"
     CDS             7878..9860
                     /locus_tag="ACGE70_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFF8693523.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(9925..10371)
                     /gene="nrdI"
                     /locus_tag="ACGE70_001897"
     CDS             complement(9925..10371)
                     /gene="nrdI"
                     /locus_tag="ACGE70_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFF8693524.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
BASE COUNT         3424 a         1853 c         2274 g         2944 t
ORIGIN      
        1 caaatgttaa attttcatac aaatatagga aacgttttac tggcttttta ctatttaaac
       61 aggctatcat aggaaaaagt gacagagaaa gtgaggcgga aaagatgaac aaaaatgacg
      121 tactgctagt tgcaaaaaat aaatcacttt attttttgcc ttccatccgc tctttagatg
      181 tttggtccgg aaaagattta ctacagtcgg aattcattaa atcagctaga caacagttgg
      241 caaattatca gctgatcgtt tttttagatt atggatttga tatggagatg gctcgacgcg
      301 tgcgcccatt taccaatgca aaaattgtgt tatttttttg gaatcacttc aaagcagaac
      361 acgaggcgat gctgaagcag gcacaagatg aaccggcaat cgatgagatt tatcattttg
      421 attttttgga agcaaaagaa cttgggctga aacataatag ttcattttat tcgaaacata
      481 tgaatttttc ttctagcgaa ccaaagacgg atctattttt tggtgcaacg aataatggga
      541 gaaaagaacg agcagaaagc tataagcagg aatttataaa aagagatgtt acgaccaact
      601 atttcattct tccacatcga gggaatgaac aacctggata tcttacttat gaagagtacc
      661 tgaaaaaaac aggggaatcg cgcgggattc tggaactgtt gcgggagggt cagcaaggag
      721 tgactttgcg gacatttgaa tccatgtatt ttcaaaagaa actagtgacg gataatcaag
      781 cgattgctca ttatcatttt tatcgtcctg aaaatatttt tttgcttcga gaacgtgatt
      841 tagatgaatt accggaattt ctgcatacac catttaagcc aatcctatca gagctattgg
      901 aattttatga tgcagaaaat tgggcaaaac gatttatcac taacgattcc actatttatg
      961 agcaatacga atatgacctc tcctctgttg aaaacaaata aaaaacctac attttgaaga
     1021 gattatgtac tgaaacaaac aagcacaatt cttatggcat catgagagtt gtgttttctt
     1081 tttgatcttt tattgggctt aaaaaaagag aaccagctgc tgattcagct gattctcatc
     1141 agagagcctt ttattctgcg ccaaggattc cttcttttgc atggactaat tgggtcaaaa
     1201 aggcacaata tggtgtagca atatcatatt tttgtccttt tcgccaaatg gcaccattga
     1261 tgtagtcgat ttcagtcaaa cgatggtttt tgatcaggtc ttggtacata gatgggtaat
     1321 gcaacccgat gttgtcagga tcataagtag aagcaatatg ttggtacact tcttcttgat
     1381 ctagtatgat tccttctttt gctgcgatag aagcaaattc agatacgatt gttcgaacca
     1441 tgctctctgc cgctttttgc gcgcctagtt cagcaatatt acaatccaaa atcgtgcata
     1501 aaccattcaa tgttccattt acacaagctt ttcgccaaat ggaataacga acgttatggc
     1561 tatagatcgg atttaaattt gcttcttgaa agacttctac aacttttttt gtaaatgctt
     1621 cgccttctgg ttccaagttc tctagttcga tttctccatc acctaacaat gtgacttttc
     1681 cgggaccagc tagaccagct gtccacatcg tgatcccaaa taagatattt ttctttggca
     1741 catatttttc cagtacatct tcatgaccta atccatttag taaacacaaa acataagtat
     1801 tgtcagtaat aatggattgg atcgaacaaa acatatcatc taattgattg gcttttgtta
     1861 atgctacgat caagtccacg tgttcgttgc tttcgatgat ttcttctggt gaatagattg
     1921 gcagttttgc gaccacttct ttaccgttaa agtcagcaat caacccattt gtacgaatgg
     1981 cttcaatgtg agcaggccaa cgatcgatca agaggacctc atttccgctt tgatgtagca
     2041 tcaatccgat tcggctgccc attgctccgg ccccagctat tgcgattttc atgttttttc
     2101 tctcctttat tcaatgagtt cccttttagt atatagaaaa atggaagaaa atagcattgt
     2161 tttgtttgaa aaaaatgaaa aaaagtgtct tacttaagaa gaaagtgagg gatacagatg
     2221 gatattttgg cggcaattga ttcatttaaa ggaagtgctt cttcatttga actaaatcag
     2281 gcagttctaa atggctggga agatcgtaaa ggaagaaaaa tcaatgtgcc gattgcagat
     2341 ggcggagaaa atactgcaga agcaatctat tacgcgctca ggggaacatg gcgtaccatt
     2401 gatgggatgg atctcttatt ccgtcagcga aaaatcagct atttattgac aacttggcaa
     2461 ggaaaaaaca ccgcagtcat cgaatctgca caagcattgg ggttagatct acttgataaa
     2521 ccaacggatc aaactatccg acttgcttcc agttatgcct taggagaact tattaaagat
     2581 gcactgcttc aagaagtaga acaaattatt gtcactttag gaggaagcgg gtgtagcgat
     2641 ggtggtctag gattactcca atccttgggt gcttctttag aagggtacac agaaggaaat
     2701 cctttattat ctactaaaaa acttcatttt gatcgaagaa acgttcttgt ttctaaagta
     2761 aaattgatta tcgctgcaga tgttaccaat ccatatacag gtcaacaagg agctgcttgg
     2821 caatttggaa gacaaaaagg aggaatagat acgacgctag ctttcttaga taaacaagca
     2881 ggaaagattg ctcagcaaat aaaagatttc tgggaggtgg atttagatga tattccagga
     2941 agtggagcag caggcggtat tggtggagcc ctttttttgt tgggagcgaa gatggtttct
     3001 ggtttcgatt tagtcagcca aatcactgga ttggaaaaaa agatcgaaca agcagatgtc
     3061 atcattacag gagaagggcg gatcgatcag caaacgatcc acggaaaagt tccatacggt
     3121 gtagcagtgc ttgcaaaaaa atatcgaaaa ccaataatcg ctatttgcgg aagtagagac
     3181 caagagctag aaggactttc tgattttctc ccaattgtct taagtattca tttgggtcca
     3241 ataggtttag aagaagcaat ggaacacagg caaacattag aaaaaacaaa aatcttagga
     3301 cagacactta gcagattttt caccatcctc ccgaagtagt tgacaaaatc attttcttta
     3361 tgctatcgta atttcaaata taggacaaag tgagggatcc gtgcattgaa aaattaagtc
     3421 gattgatgaa agctgcattc agactgtacc ttgtggagac aaggaagcag tgtgcagatt
     3481 ttcgggattg gcttggtttt ttcgatgtat gggtcttttt gtgtgtatcc aacccttgca
     3541 tcctctttag ccagttctga aaataggact ggtctttttt tacgaggtga tggaaaatgt
     3601 ctaaaatcga aataaaaaat ctgacattcg gctacgacag ccaaggcaca ttattatttg
     3661 aacaagcaaa tctaaatttt gacacacaat ggaaactagg acttatcgga cgaaacggtc
     3721 gaggaaagac aactttactg aatattctac aaaacaaact accttatcaa gggcaagtaa
     3781 tccatcagca agaatttgcc tatttcccgc aacagacaaa agataaagaa cgtttaacct
     3841 attacgtgtt aaatgatatt acggattttg agatatggga aatcgaaaga gagctccaat
     3901 tgatgcaaac agatcctgaa atcttatgga gagaattcag cacactatcg gggggagaga
     3961 agacaaaagt cctactggca cttttatttg tggatgacac tcatttcccg ttaatcgatg
     4021 aaccaacgaa tcatttggat atctctggta gaaaacaagt agcggcttat ttgaaaaaga
     4081 aaaaacaagg cttcatcgtg gtcagccatg accggggatt tatcgatgaa gtagtggacc
     4141 atgttttagc aatcgaaaaa agtcaactgg aactttatca agggaatttc tctatctatg
     4201 aagaacagaa aaaacttcgt gatgaatttg aaatggctca aaatgaaaaa ttgaaaaaag
     4261 aagtcagtag gctaaagaaa acagcagctg aaaaagccga atggtctcgt tcccgagaag
     4321 gagataaaac aaagaaacaa gtcggattca tcgatactga atctagacga gtgaataaag
     4381 gagcagtggg tgctgatgct gcacggacga tgaaacgatc caaagcaatc gtgaatcgga
     4441 tggagaccca gatcagcgag aaagaaaaac tattaaaaga tatcgaatat atcgattctt
     4501 tgacgatgaa tagccaagcg tctcaccata agcgactttt aagcgtagaa gatcttcaat
     4561 tagggtatga aaatctgtta ttcgagccaa ttcattttac aatcgagcct catcagcggg
     4621 tggcgatttc aggtcctaac ggtgcaggaa agtcatccat tatccattat cttctggggg
     4681 cattcaacgg caaggttata ggagaaaaat accagccaaa acatctgagc attagttatg
     4741 caagccaaaa ttatgaagac aatcgaggaa cgttggcgga atttgcagag aaaaaccaag
     4801 tagactacca agcatttttg aacaacctcc gaaagcttgg gatggaaaga gatgtttttc
     4861 ataacaagat cgagcagatg agtatgggcc aacggaaaaa agtggaattg gctaaatctt
     4921 tatcacagcc agctgaacta tatacatggg atgaaccatt gaattatttg gatgtcttca
     4981 atcaagaaca attagaacaa ctgatcttga acgtgaaacc tgccatgtta ctagtggaac
     5041 atgatcaaac cttcctggat aaagtatcta ctgagattat ttctcttgag agaatctaac
     5101 ccttgtaatc tcaagaacaa caaggtatgg ttaaggggaa ctttgccaac gaaggagaga
     5161 atcacatgct ttatgtaacg agacacggag aaacaacttg gaacgcacaa ggattagttt
     5221 gcggacgggc agatgtgccg ctgactgaaa aaggacagat gcaggcacag aaactagcag
     5281 aaaaggtagt agatttgcct gttccaatca cgaaaatcat ccattcccct cttcaacgag
     5341 caagagatac agcacaagca gttgctgatc gtctcagctt gccgctgacc gttgatgaac
     5401 gattggtaga aatggatttt ggggattatg atggactgcc aagcaaagat gagaactttc
     5461 aaaaagcacg tttagcgttc gctgtacgat tcccaaatgg ggagtcagtc ttagatgtgt
     5521 atgcacggat cgttccttta ttgaaagaat gcatcgaaga cgaagaaaat gtctatctgt
     5581 tagtttgcca taatgccttg ataagagtga tcaatgctta ttttcatccg atgcctaatg
     5641 aggggttctt tacattcatg gtagataata cagaattggt ttcttatgag taattatgtt
     5701 tttgtgttat ttaaataaaa tatttcaagg cactgctttt gcttagcagt gcctttttta
     5761 ggttatgaca attcttttga taatataaac tcaaccttta gaaatgaaat ttaattattt
     5821 taataaaatt attgaaattt tgtttcttta gatatatact ctagtcatag aaatgaagac
     5881 ggatacagaa aaagagggat aatatgtttg gcttttcaat atttatgaac gattctttgt
     5941 cagaagagaa gaaacagtat atccgccaaa tggcatccaa tggctttgtc ggtattttca
     6001 cttccatgca tataccagaa gatgatgttt cggcatatca aaagcgattg atcgaactag
     6061 gaagcgaagc gaaaaagaat aaactagaat tgatggtcga tatttcagga gaggcattgg
     6121 aacgagcggg tttttcatta gaagatctaa cgcctttgaa agcaattggt gtaacaggtt
     6181 taagaatgga ttatcatatt tccaatcagc agattgccga ctggtcacat caattgaaaa
     6241 tcagtttgaa tgctagcacg atcacgccta aagatatcga tgaattgaaa gaagcagaag
     6301 ctgatttctc acagattgaa gcttggcata attattatcc acgtcccgaa acagggctgg
     6361 acaaagaatg gtatcagaaa aaaaatcaat ggctcaaaat tcaaggtctc cttgtccaag
     6421 gttttgtacc aggagataca gagttgagag gcccactata tcaaggattg ccaactttgg
     6481 aagaacatcg agggatccat cctcttgcag gcgctctaga tctattagca agtaacacgg
     6541 atattgttta catcggagat gcaggtctgt cagaaaacgt tcaggagcaa tttgcttctt
     6601 tccaaaaaga acagacagtc ttgctccata cggaacctgt agatgaagaa ttttacgaat
     6661 atatattagg gaagcatacg aatcggcaag acgatgcaag agatgtcata cgaagcgtag
     6721 atgcacgttt tcgagagatt ccgcctatcc cagcaagaaa tacagctacg cgtatgaaag
     6781 ggtctattac tttagataac gaaaagtatt tgcgctatat gggtgagatc caattaacca
     6841 aatatgattt gccagcggat gaaaaagtga atgtagtagc taaagttatc aaagaagagc
     6901 taccattgat caaccaaatc aaagctggta tgaactatca atttataaga aaagaaggaa
     6961 gataaaaatg agcaaaatca atttagagaa cctaacaacg gaacgcagaa accaagaaac
     7021 ttttggatta gatgagatga gtatttctca agccgttcaa ctaatgaaca aagaagatca
     7081 gaaagtggcc gctgcggttg aaaaagaact gacaagtatc gaaccagtga ttgctaacac
     7141 gattgagtcc tttaaaaaag gtggacgtct gatttatatg ggagccggaa caagtggaag
     7201 actaggtgtg ttagatgctg ctgaatgcgt accgacattc ggtgtagaac cggaaatggt
     7261 cataggtcta attgctggtg gagaagaagc aatgactgta gctgtcgaag gggccgaaga
     7321 ttcttatgag ctagggcaag aagatctaat caagctgaat ttgacaaaaa atgatatagt
     7381 tattggtatc gcagctagcg gtcgtacacc atatgtgatc ggtggattag attacgcaaa
     7441 atcgatcggt gctgtcacag gaacgatttc ttgcaataaa caagccgaaa tcagtaaaca
     7501 cgcggatttc ccaatcgaag tcgatgcagg tccagaattt ttgacaggct ctactcgatt
     7561 gaagtcagga acagcacaaa aacttatctt gaatatgatt tctactatca gtatgattgg
     7621 tataggtaaa gtattcaata acttgatggt cgatgtcaaa ccaacaaatg aaaaactaat
     7681 cgaacgctca aaacgaatca tcatgcaagc gacagaagct gattacgaaa cagctgaaaa
     7741 gtactttgaa gaagcagatc aaaatgttaa gctggctatc gtcatgattt tgacaaatag
     7801 tgataaagag gaagctcaag caaaattagc tgaagcaaac ggatttgtaa aagggacctt
     7861 atgaggggga aaagaccatg gcaaaaaacg aactgacttt ggaagaactg gcaaaacaaa
     7921 tttataaagg ggcaggcggc atgggcaatg tagaaagcat tgtccactgc atgactcgtg
     7981 tgcgtatgaa gatcatcaat gatgatcttg taaatgtctc tgaattaaaa gcgattccag
     8041 gagtgcttgg tgtagtagaa gatgaacagc ttcaagtcat catcggacct gggaaagtga
     8101 acaaagtagc acagcaaatg gttgatttgg caggcgtgaa attaggagaa aaactttctt
     8161 ctgtaagtac agctacagca aattcatcag catctggaaa agaccgtgta aacgaacggg
     8221 cacaagaaat gaaagcggca caaaaatcga aacaaaaacc atctaaaatc aaatcggtat
     8281 taaaagacat ttctaatatc tttgtgccaa tgattcctgc atttgtcggt actgggatcg
     8341 tggcagggat tgccgcagtt ttagcaaact tagtcacagc cggaacactt gatgcagcaa
     8401 catggcaaca atatatcgat gtgatgaaca tcttgaaaaa cgggatgttt agctacctag
     8461 ttatctatac aggtatcaat gctgcacaag tttttggtgc aacaccaact cttggtggag
     8521 ttatcggggc cgttgtgatg ctgacaggga tgaatccgga agcaccactg aaaaacttat
     8581 ttactggtga agctcttgca gctggacaag gtgggatcct aggtgtcatc ttcgctgttt
     8641 ggctattgtc aatcgttgag aaaaaactgc ataaagtagt acctgatgcg gtagatatca
     8701 tcgttacacc aactctttca ttgattgcca tcggtctgat tgaaattttc ttcatcatgc
     8761 ctttagcagg tttcatttct gatggcatgg tcggtgggat caactgggta cttggcgttg
     8821 gtggagcatt tgccggcttc gttctaggca cattattctt gccgatggtt atgttcggac
     8881 tgcatcaaat tttgactcca atccatgtgc aaatgattga tgaaactggt cgtacgctat
     8941 tgcttccaat tcttgcgatg gctggtgcag gacaagtggg tgcagcattg gcattatgga
     9001 ttcgctgcaa aaaagataaa gaattaacag aaatgatcaa aggagcttta ccagtcggga
     9061 tcctaggaat cggcgaacca ttgatttacg gagtaacatt acctctagga cgcccattta
     9121 ttactgcttg tatcggtgga ggtatcggcg gggcagtgat cggaatgatc ggaaatgtcg
     9181 gggctatcgc tattggacct tcaggagccg ctttgatccc attgatctct gacggtaaat
     9241 ggttaggcta catccttgga ttattggcag cttatgctgg tggcttcgtt gctacattct
     9301 tcttcggtat tcctaaagac cgtttagaaa aagaagaatt agcgactgaa acagccacag
     9361 taaatgaagt gcctgaaaaa gtagtacctc aaacttcagc tgaaagtgtg actatcctct
     9421 catcagttgc tgacggaaca gttgtttctt tagataaagc aagtgatcca gtttttgcac
     9481 aaaagatgat gggtgaaggc tactttgtag aaccagaaaa cggacatatc tattctccag
     9541 ttactggggt agtcagctct gtcttcccaa cgaaacacgc tattgggatc acaacgccaa
     9601 atggtttaga aatcttgctt catatgggaa tcaacacagt tgacttaggc ggtacgccat
     9661 ttgatgtgaa agtaacagaa gggcagcaag tgacacctga tacgcttgta gcagaagctg
     9721 acttagaagc gatcaaagca gcaggcaaag aaacatccat gatcgtcttg gtaaccaata
     9781 tggatcgtgt aaaaaacttt gtcttggaaa aaacaggtaa agcaaaagca aaagcacctg
     9841 tgatggatgt caaagcttaa tatgatagaa acaatagaaa cgaaagaaga aactgaaatc
     9901 tatttttcag cttcttcttt tttattattt attgttttct gctaaaatct gataaattcg
     9961 tgtcacatca gatggtgttc cccgtaattc ataatctgcc aagaaaggaa agccaaatgt
    10021 ttgtgcatat tgtttggctg ttagacaata ctgattattg aagtttttat ttccgctacc
    10081 gactacacca agacaaagtt tatgattgtc ttcatgttcc agatattcac gcatcgtttc
    10141 ggtcaagatt tctgtatcac cgttatctaa accatttccg ccgtctaaat aagtaggaac
    10201 gaaagtaaaa aatggttctg tttctttcgt aaaatcacta ttttcatgga tttcttttaa
    10261 agtaacttca ggattgacag gatcttctgc atgcattttt tcagcatatt ctgctagatg
    10321 tttggcaaag gcacgcgtat ttcctgaaat cgatatatat aaaatgttca tggatcatcc
    10381 tccaattctt tccttttagt atagcatctt ttttaagttt tttggtggtg taatttggaa
    10441 agggactagg cgctttgctt gtataatgag aaaaaagccg gtagaattat atttc
//
LOCUS       DAXWOO010000096        10431 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.096, whole genome shotgun sequence.
ACCESSION   DAXWOO010000096 DAXWOO010000000
VERSION     DAXWOO010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.096"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(242..754)
                     /locus_tag="ACGE70_001898"
     CDS             complement(242..754)
                     /locus_tag="ACGE70_001898"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFF8693525.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(768..3065)
                     /gene="recJ"
                     /locus_tag="ACGE70_001899"
     CDS             complement(768..3065)
                     /gene="recJ"
                     /locus_tag="ACGE70_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFF8693526.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3207..3650)
                     /locus_tag="ACGE70_001900"
     CDS             complement(3207..3650)
                     /locus_tag="ACGE70_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFF8693527.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3651..4439)
                     /locus_tag="ACGE70_001901"
     CDS             complement(3651..4439)
                     /locus_tag="ACGE70_001901"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFF8693528.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4436..5377)
                     /gene="rnz"
                     /locus_tag="ACGE70_001902"
     CDS             complement(4436..5377)
                     /gene="rnz"
                     /locus_tag="ACGE70_001902"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFF8693529.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5554..6141)
                     /locus_tag="ACGE70_001903"
     CDS             complement(5554..6141)
                     /locus_tag="ACGE70_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFF8693530.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6362..7675)
                     /gene="obgE"
                     /locus_tag="ACGE70_001904"
     CDS             complement(6362..7675)
                     /gene="obgE"
                     /locus_tag="ACGE70_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFF8693531.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7972..8982
                     /gene="gap"
                     /locus_tag="ACGE70_001905"
     CDS             7972..8982
                     /gene="gap"
                     /locus_tag="ACGE70_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFF8693532.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9067..10002)
                     /locus_tag="ACGE70_001906"
     CDS             complement(9067..10002)
                     /locus_tag="ACGE70_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFF8693533.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            10188..>10431
                     /locus_tag="ACGE70_001907"
     CDS             10188..>10431
                     /locus_tag="ACGE70_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8693534.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIK"
BASE COUNT         2922 a         2352 c         1805 g         3352 t
ORIGIN      
        1 cgggggtgtg acaaaaatcg gtcacatcct ttttttcgaa taaacggtgt tctaaagcca
       61 gtttttcggt attaagtcaa attgcggaaa aagtgatttt ttaaacttct actggtcttt
      121 gcttgtttcc atttataaaa aaatagcgac cctcaaaagt cagatagaga atctgacttt
      181 tgagggtcgc tttaaaatcg cttctttaag aataacatca agaatagctt cttttttctg
      241 cttaatattc cattaatgta atgatgtcat atcctttgat tttgtcgcgt ccatggagat
      301 ccatcaattc gatcaaaaat gcgcagccta cgactacacc gcctaattgt tcgatcagtt
      361 cgatcgtcgc tttgattgtt cctccagtag ctagcaaatc gtcacaaatc agcacacgtt
      421 gacctggttt aatggcatcc ttatgcaatg tcaatgtatc tgttccatat tctaatccat
      481 aagtcacttc gatggtttct cgaggtaact tccctttttt gcggactggg gcaaaaccga
      541 ctcctaactc ataagcaaca ggacatccta cgatgaatcc acgggcttct ggcccaacta
      601 ccatatcaat tcgtttttct tttgcataat ttacgatttg tttagtagct tcccgataag
      661 cgtcaccatc tgccatcagt ggggaaatat ctcgaaacac aattcctttt gacggatagt
      721 cgggaatact ggcaatataa tcttttaaat ccactctttg tttcctccta ggttgttaac
      781 catttcttta tggtaggaat atcgctcaaa agcaaaaatt cctcaacttt gattttattg
      841 agccgtcgct ggtataatct gctctcagtc aaaggatgac tttctgggct agcgactttt
      901 tgtaaaacac cgttctctat tgtaacaaat ttcaattcaa aaaacacctg tatcataaaa
      961 attaataatt tttcagggat acgcaaatat tgagctattg cttttatttt atgacgtata
     1021 tcaattcgtt cttgagaatg gatcaattta aacaattttg cgtactgttc tcttgtgcct
     1081 accccattca aataggcctc atcaggtgaa atccccatca gataaacacg attgaatgaa
     1141 taagaccgaa aaacttcttt cagatccgct aaatcatctg gacaatccat aacaactaga
     1201 gagtttggtt ttcttttttc aattattgcc tttacagaat ctagtccttc gaaaacaatc
     1261 gcagcttcct ggataatctc aggcacgaat ttcaacgtcg aagaatcaaa tgctaaataa
     1321 aggctacctt ccgacgttcg gctctgttca cggaaacgtt ttgcccgcca atcaaatact
     1381 tgcagaccac ccacagaaaa atcagatacc atcagctgcg gtttcttacg cccgttccat
     1441 tcattgatcg aaagctttcc taccacatct acctgattat tcagcagttc ttcttcttga
     1501 gtaccaaaac caaaagcgac cgcatcaagc tgactcgatg catcggaaag gacaaatttc
     1561 agatgctgtt ggtttgctcc gattcttttg atattttctg cttggacttg tcgaaataaa
     1621 aactgaggca aaggattatc cgtaccaaat ggcgccaata gtttcagctc atcaattcgc
     1681 tctactgtta cttcatttgg cagcaatact tcatcaattc tcaactcagg ccctctcatc
     1741 aagtcaattt gatgatcgac tatatattgg ttcatcttct cctgtagtat agtgacattt
     1801 tcacttggca tcgttaaacc gactgctgca tgatgtcctc cgaagtaagt aaataaatct
     1861 cgcatctggt caagcatttc aaaaagattc aaggcttcaa tactacgtcc agatccttta
     1921 gctgatccat cttcttttaa agtaaggaca agcgttggtt tacccgtttc attcattatt
     1981 ttcccagcaa caatgcccaa aacgccttca tgccatcctc ggtttaccaa tagatggata
     2041 tgattctcct tattgaccat cgctaatgct tcttgtgtta tctgctctac gattgctttt
     2101 ctttcttcat tgatctcatt caattttttt gcttgaacgg atgcttcctc ttcatcaaac
     2161 gtcgcaagta acgagacggc aggacgtgga tcgcccatcc gaccgattgc atttagtctg
     2221 ggagcaatcg aaaaaccgat cgttgtttcg tctgcgtccc tcatttttac gccgctttct
     2281 tcaaatagcg cctgaagtcc tatccgttct gaatgatgaa tggcatcgat tcctaatgaa
     2341 acaaggattc gattctcatc agtcaatgaa actaaatcgg cgatcgttcc tatcgctact
     2401 aaatccaaaa attctgcagg cggttcttcc aataatgcag aagctacttt gaatgctact
     2461 ccgactcctg ccaaatctgg aaatggatag tgccccgctg gatgacgagg gtgaacaatc
     2521 gcataagcgt ctggtaaatt ctcaggtagt tcgtgatgat ctgtaatgat cacgtctaca
     2581 ccagcttgct gagcgtatgc taccgcatca tttcctgcta ccccgttatc aacagtgatg
     2641 atcaactgga ttccttcttc gatcttttct tggtataccg attggtttgg tccataccca
     2701 tgctcaaatc gatttggcag gaatacttct acttctgcac caagtaattc cagtgtttct
     2761 ttcattaccg ttgcactggt gatcccatct gcgtcataat caccgtaaac gaggattttt
     2821 tctccactga ttacagcttc ttggattcgg ctgattgctt tatccatatc gtgtatcaga
     2881 tacggatcat gtaattcttg ttcctttgga tgtaaaaatc ggttgatatc cttttcctca
     2941 tggtaacctc tttgccacag taacttaccg acaaatggag ataatttcag ttcacggatc
     3001 atttggagaa aggattcaga aggctctgtc tcgcttaact tccaatcaaa atttgcttgt
     3061 ctcactttct cactcctaac ttagttatta tagcaaagga aatcagcaaa aaaaagcaga
     3121 aagaaagaat aaaaacagat cagatccgct agacagatgt tcagcaggac ctgatctttt
     3181 gtacgaccat ttatgaaata tccctattag tcaaaataat tgaagctttg tttgggcgat
     3241 tgagtttttt gttcttccgg cgctttcaat tgactgattt gtgcggtttt cgcttgtaac
     3301 tccgcctgat aagcagcttc caactccgca cgttcgtttc taaactcgcg ttgctgttct
     3361 tcttttgctt cttcaatttt cttttgaagt tctgtttgat attctgacat ttcctgagtc
     3421 agctgtttga tttgcttttt ttgctgccat agtgttgccg aagcagtcaa aaatacaatc
     3481 agtgcaccaa tcagcgcaga accgatgatc actaagatca atggcgcaga aaaggaagtg
     3541 aagccaaaac ttacaggaac atttttattg ttcaatacag caaaaagaac aataatcaaa
     3601 actaaaacaa atccaagaat cacattgctt tgctttttca attccttcac ctactttttg
     3661 ttaaaaattc cgctggctaa ataatcacca atcgttggaa acagcgtata aaattttgat
     3721 gcaacttcta aaataaacgg ttgattgatt tcccgttttg gatgtctcat attgcggacg
     3781 atcatttttg ccaacttggc aggttctaaa acgatttgtc cgactttttc caaatagctt
     3841 ccgcttggat cagccttatc gaagaaattt gtctcgattg gacctggatt cactgtcgta
     3901 acagctacgc caagtggttt caattctaat cgcaaagcgt tggagaagcc aaggactgca
     3961 aatttcgtgg cagaataaac cgttgatttt gcagttgcca tttttccagc catagaggcg
     4021 atattaatga tatgaccttg tcttctttct gccatatcga tcgcaaattt ttgtgtcaaa
     4081 accatcattc ctaagatgtt gacttcaaac atatcgtatg cttttccaag gtcgaatgta
     4141 aggaagtctt cgaaaatccc aaaacccgca ttgtttacca gaatatcgac gcgcccgact
     4201 tcttccatca ccttttcata taattcgtcc acgctttcag gattgctgac gtctaccttg
     4261 aaagcataag cctctttgcc gctcaagcgt ctgcactcgt ctctgacacc ttctacaaat
     4321 gccattcttc gtgcgcaact tataacgatt gctccttgct ttgctgcttc ataacagatt
     4381 tgtgcgccca gaccgcctga actacctgtt actaaaacaa ctttatcttc taaattcatg
     4441 cctcttcctc ctcgcgaaat ggaatctcaa tgctatcaaa atctttcata atcgtcgtgt
     4501 tttcaaacac ttcttgtgct tctttttcaa gctctaaagc tgcttttcct aaatatctcg
     4561 cactgatatg agtaagtagc agccgttttg cctgcgcttc ttttgcaact tcagcagctt
     4621 gatgagtcgt ggaatggaaa tagttgtgcg ccattcttgc ttcatcttta ttaaatgtgc
     4681 tttcatgtac cagaatatcg ctattttccg caagcacaac ggaattatgt gtttttctcg
     4741 tatcgcctaa aatagtcacg attcgtcctt ttttatctgg acctacgaaa tcttttccat
     4801 tgatcgtccg cccatcttct agttggatcg tttcaccttg cttcagtttg ccgtacagtg
     4861 gtccagctgg aatacctaat tcttttagac gatccacctg caattctcct ttatgatcat
     4921 gttcaaccac acgataacca aaactgtcga tcccatgatt tagttttttt gtataaacag
     4981 aaaattgctg atcagtaaaa atcggggctg tttcatttag ctcaatgaat ttcagcggat
     5041 aacttaaacg cgtttgcgaa atacctaaag agacttttat gtattcctct atcccttttg
     5101 gcccgtaaat ctctaatggc gtatccccac cttgaaatga ccggctgctg atgagcccgg
     5161 gaagtccaaa aatatgatcg ccgtgtaaat gtgtaatgaa aattttaccg atttttctcg
     5221 gacgaatagt cgttcgcaaa atctgcattt gcgtgccttc gccacagtcg aacaaccaaa
     5281 cttcattgcg ctcgtctaaa agtttcaatg ctatacttgt cacatttcta tgttttgcag
     5341 gaacacctgc acctgttcct aaaaattgta attccataat ctacctgact ttctttaagt
     5401 tcctctttaa ttttagggta aaaagaaggg ataatcaacc gtaagtcgag aatgggttca
     5461 aattaaaaat aacgttctaa agcgatagcg ttttctttga aaattactat aaaaacagca
     5521 gcgaacccgt ttgagatcct ctgctgtttt ttattaattt gaagatccag aactttcatt
     5581 gtcgttggaa gtttgcggat gtttgtcctt ccaagtcttt tgtgcagctt ctttcgcatg
     5641 attttcagct tctttcgcat catgcacatc tttttcgaca tcttcataat ccaagcgagt
     5701 aatttctccg cctaattcca tcattaccaa ttgattcagt gggcgattgt cctcttcatc
     5761 agcaatcaag atcagtcctg tagctccttc accgatttta tttcctacaa agtcgaacac
     5821 ggtctgggca tcttttattt ctttggcatc ttgtgtcgca ccgatcattc ctccggcaaa
     5881 ccatcctaat aaaatcccta gcggaccacc taatacacca accaacattc cgattaatcc
     5941 acccgtagaa gatttgttat taccggtaaa gtcgataaaa tcttctattt tgaattgatg
     6001 tgtcccatta ggatcatggc tgacaactgc catctgttct cctttaatcg aacgaacagc
     6061 atgtaacttt ttgatttctg aaaatgcctg atatgcctgg ctttctactt gaaaatttaa
     6121 aatgattact cgtttgctca ttaaaaacgc ccctttcata ccttcttgtt atttatttta
     6181 cctttaattg aaaaataaga aaagaaaagc actctttttt tccaataaaa tagggctgtg
     6241 acaaaagtcg tatcacagct cctttttccg aataaacggc gttcttgaag ctgatcactt
     6301 ctgtcacaac ctcccttaaa tctgaatcta tattttttgg tatttgtttt tatttgcttg
     6361 cctattcgac aaattcaaat tcaaactcac cgatacgtac aaggtcgcca tctttcgcac
     6421 cgcgtgcccg aagagcttca tccacaccca taccacgtaa ttgacgagca aaacgcatca
     6481 ctgtttcatc atgatcgaaa ttcgtcattt ggaataattt ttcgatcttc tcaccagata
     6541 agatccacgt tgcatctgaa tcacgatcga tttggaactc aggaccttct ggattgaatc
     6601 catagtgtac agtttcttct tccagttctt cttcgtacaa tgggaattct ggcgttactt
     6661 ctaacaaatc tgctgtagca ttcaataacg cgtccaatcc ttgtcgagtc actccagaaa
     6721 ttggaaatac tgggatgtca tcagcaaatt catcttcttt ttccttgttc agctgttctt
     6781 tgaatttcac tagattttcc ggtgcatccg gcatatccat tttgtttgcc acgatgattt
     6841 gcggacgctc tagcaagcgt aagttataag tagataattc tttgttgatg gcaagatagt
     6901 cttcataagg atctcgtcct tccatcccac tcatatcgat cacatgtaaa attactcgtg
     6961 ttctttcgat atgacgaagg aattgtgtac ccagcccaac accttgcgaa gctccttcaa
     7021 tcaagcctgg caagtctgct gcggcaaaac ttctgccatc acttgtagta accattccta
     7081 gattaggtac gagcgtcgtg aaatgataag cgccgatttt tggacgagca gatgaaataa
     7141 cggatagtaa agtcgatttc ccgacagatg gaaaacctac aagtccaacg tctgccaata
     7201 ctttcaattc tagttcgatt tttcgctctt gtcctggttc accgttttca gcaatttccg
     7261 gtgcaggatt ccgaggagag gcaaaacgga tattccctcg tccaccgcgt ccaccttttg
     7321 ccacaactag tgtttgacca ttttcaatta aatctcctaa aagagcacca gtctccgcat
     7381 ctcggacagt ggttccttgt ggtactttaa cataggtatc ttctgaacca cgaccgtgca
     7441 tccctttact catcccattt tcacctggct gcgctttgaa atggcgattg aaccgaaaat
     7501 ccatcaatgt acgtaaccct tcatcgacga tcaagatgac atcgccgcca cgaccaccgt
     7561 ctccgccagc cggtccgccg tcaggaacgt atttttcgcg acgaaatgca accattccgt
     7621 ctccgccctt gccagctttc acatcgatcg ttacttgatc taaaaacatg gacataatta
     7681 agtcctccta ttattcattt atttatttga ctgcctgaat ttccacaata aacaaaagtc
     7741 ttacttattt tatcggtgaa atctgatttt gtctatcatt tcagcaaaaa gattgtttac
     7801 cttcttcttt ttttatcatt tggtaaggtt tccaaccttt tttctgctac caagtataat
     7861 tttttgacaa aatattcttt gcgtagcaga ataacagtgt attaattgta ttaattgtat
     7921 taattgagga tgccgtttca cagcacccct aataaaggag gaacttttga tatgacagta
     7981 aaagtaggta ttaacggatt tgggcgtatt ggccgtctgg ctttccgccg catcaaagaa
     8041 gtctcagatg atattgaagt agtagcaatc aacgacttaa caagtccaac tatgttagca
     8101 caattattgc aatttgactc tactcatggt acttatcctg gcacagttac tgcaacagaa
     8161 gactcaatcg tagtggatgg cgaagcaact cgtgtttatg cagaacccga cgcaagcaaa
     8221 atcccttggg caaaagaaaa cggtgtagat atcgtactag aatgtactgg tttttataca
     8281 tctgaagaaa aagcaaaagc gcatttagat gctggtgtca aacgagtagt tatttctgcc
     8341 ccagctggtg ctatgaaaac catcgtctat aacgtcaatg atgatacatt agatgaaaac
     8401 gacaaaatca tctcagcagg ttcatgtaca acaaactgtt tagcacctat ggcttatttc
     8461 ttaaataatg aatttgggat cgaagttggt acaatgacaa cagttcatgc ctacacttcc
     8521 acacaaatgc tcttagatgg acctgtaaaa ggcggtaatt tgcgtgctgc tcgttcagct
     8581 gctgacaata ctatcccaca ttctactggt gctgcgaaag caatcggctt agttatccct
     8641 gagctacaag gaaaattaca aggacacgca caacgtgttc cagtcgttga tggttcatta
     8701 actgaattgg tttcagtatt gaaaacaaaa gtaactgctg atcaagtaaa cgaagcaatc
     8761 aaaaaacaca cagttgacaa tccttcattc gggtatgatg accgtcaaat cgtttctagc
     8821 gatgtcattg gaacaactca aggttcgatc tttgacccaa cgcaaacaga agtaacaact
     8881 gctggtgatt tccaacttgt taaaactgtt gcatggtatg acaatgagta tggatttact
     8941 tgccaaatga ttcgcttact agaaaaattc gctaacttgt aatttgatag atcaatcgat
     9001 aaaagaagcc tttgactgga tccagtcaaa ggcttctttc ttttcatccc ctacttttaa
     9061 actcgttcaa tgaacatatt ggcttaaaaa ttgttggact acttgctgat atttttcagg
     9121 atctgtcttg tatgcttccg catgctcggc tccttttaca acatactttt cttttggtcc
     9181 cttcgttgca cgatagacct tatctaacat ttcaaatggt acaaatgtat cttcgtctcc
     9241 atgtataaag agcattgggc gctggttttt cttcaattgt tccactgcat ttgcttcacc
     9301 gaaaaagtag ccagcacgga cttttgtcac tagactcgtt acttgcataa gcggaaatgc
     9361 tggaagacta aacatatcgt ctagctgata agcaagctca tcatgaacgg aagaataacc
     9421 gcaatcttca atgattgctt tcacattatc tggtagtttt tctccgctag tcatcataac
     9481 tgttgctgct cccatgctga caccgtatag aacgatttct tgcgattttc cattgttttc
     9541 tagcactttg tttatccact gtacatagtc tttacgctct ggccatccaa atccaatgta
     9601 atccccttcg ctttttccat gtcctcttgc atcaggaaca aggacattgt aacccatatc
     9661 atgaaacatc tttgcgtaat tggtcatcgt ttcagcgttt cccatatagc catgagccac
     9721 aagagctgtt ttttcacttt tcgtttcagc aggaagataa atcgctgata atttcagacc
     9781 gtcttttgaa gtgatggtcc aatcgcttcg gttttctttt gaactaaacc atttatgtgc
     9841 atccgtttct ttggatgtct gtgttgtacc aggtgtatct tcgtctaaaa agtctttctc
     9901 agaaggcaca acagcataat tataaaaata attccctgca aacactaacg caacaatcag
     9961 agctaaagcg acgcctaaaa ggattttcat ccaaagtttc ataaatcatc catctcattt
    10021 ctcttttttc atataacctt gaattagtat aatcaaagaa tctctactat gcaatgttca
    10081 tttttataac ccgcgaattg taaaattaag ttagaaaaat aaaaaggcat ttatggtaca
    10141 ctcaaattgt atttccgacg aaagaaaaca aaggagtgta accataaatg acctacaaac
    10201 atcttaccat agacgaactg acaatgatag aatcatatta tcttcaacat aataagccgg
    10261 ttgaaatcgc taaccgaatg ggacgtgcta tacaaactat ttataatgta gtcaataagt
    10321 tcaagcaagg caagactgct cttgattatt ggcaccagta taaagaaaat aagaaaaaat
    10381 gtggtagaaa agtcattcaa ttacctgctc atgaagtaga ttacattaaa g
//
LOCUS       DAXWOO010000097        10397 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.097, whole genome shotgun sequence.
ACCESSION   DAXWOO010000097 DAXWOO010000000
VERSION     DAXWOO010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.097"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(194..772)
                     /locus_tag="ACGE70_001908"
     CDS             complement(194..772)
                     /locus_tag="ACGE70_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFF8693535.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(792..1460)
                     /locus_tag="ACGE70_001909"
     CDS             complement(792..1460)
                     /locus_tag="ACGE70_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8693536.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(1475..2545)
                     /locus_tag="ACGE70_001910"
     CDS             complement(1475..2545)
                     /locus_tag="ACGE70_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8693537.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            complement(2716..4080)
                     /locus_tag="ACGE70_001911"
     CDS             complement(2716..4080)
                     /locus_tag="ACGE70_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFF8693538.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(4077..5636)
                     /locus_tag="ACGE70_001912"
     CDS             complement(4077..5636)
                     /locus_tag="ACGE70_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8693539.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(5692..8172)
                     /locus_tag="ACGE70_001913"
     CDS             complement(5692..8172)
                     /locus_tag="ACGE70_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693540.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(8156..8491)
                     /locus_tag="ACGE70_001914"
     CDS             complement(8156..8491)
                     /locus_tag="ACGE70_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693541.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(8505..9539)
                     /locus_tag="ACGE70_001915"
     CDS             complement(8505..9539)
                     /locus_tag="ACGE70_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFF8693542.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(9629..10309)
                     /locus_tag="ACGE70_001916"
     CDS             complement(9629..10309)
                     /locus_tag="ACGE70_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFF8693543.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
BASE COUNT         2994 a         2128 c         1807 g         3468 t
ORIGIN      
        1 attaatttct tcgttattaa attaaaacac ttcctggaac accgacataa taaataaaaa
       61 tataccctga taatcatgag agaataacaa atttgtattt atgaaaaact acctatataa
      121 aaaaaacaag atataacgat cttcgtcata tcttgcttct tcacctaact atttttctcc
      181 ctatataaca gctttacgct tctcctaata caccattcaa atataaatcg atcaattgcg
      241 ccacatttag cggttcttcc acaggtcttt tcgaccgagt gatgtaaggt gctaacacta
      301 agaaaaaatg ccttgctgcg atttcttttg aaatcccatt tcttagcaca gattggtttg
      361 ctgaaaagat ttcttcaaaa ggttcacgat aatttttctt ccatagaaag aaaagatgat
      421 ctcttgtttc ctttgaaaga ttgctttcaa gatcgtgaag catcagaaac aaatcgacca
      481 gatgtgtctc aatcaaaaac tgcgccattt ttgtcaaggt tacttgaaat cccgtttcgc
      541 ttgtttgaac gatttctctc aaagcttttc caacatcttt taagtggttt tctaaaactg
      601 ccgtatatag tttttcttta tcaccgaaat aatgatagag attgggttgg gtgatcccaa
      661 ctttttgtgc gatctctctt gttgaagtgc cgagatatcc cttggacata aacaagtcag
      721 tcgcttcttt caaaattttt tgctgtgtat ctttgttgta tcgatggacc atttttccac
      781 ctgctttttg gttattcttt ttctttcaat acaccatctt ccatcataaa tattctatcg
      841 catttgtcaa tcagccgtgt atcgtgagta accatgatcg ttgctttatt tttgtctttt
      901 gattcttttg ccaagatatc gactacttct cgtgcctttt ctgaatccaa actagcagtt
      961 ggttcatctg ctagaataat ggacggatca ttgtacaatg cacgagcgat tgcgaccctt
     1021 tgccgttctc caccagatag ttcttctgga tacttgttga tcaacttatc cacgccaagc
     1081 tgctcaaata attcttttgc ttctttttga cgatgttcat gtttgatctt atctactaat
     1141 aataactgct tttttactgt cagaaaagga accagattgg aagcttgcaa aataaagcca
     1201 atttctttaa aacgtagttt aacgcgttca ctttcttttt gattactgaa ttcttgatca
     1261 ttgataaata cagagccact gctaggcgtc tgcaatccgc ctgccaaagt caaaaaagta
     1321 cttttacctg aaccagaagg accgatgatt gcaacaaatt cccctttatc gatactcatg
     1381 gttgtttctt tcaatgcttt aactacttgg tcgccatctt ggaaactttt ttctacttct
     1441 ttaaattcta ttgctttcat gctcttcctc cttcttatcc aattgccttc aacggatcga
     1501 tttttacgat tgtccgaaca gaaaatagtg cgcctaacac agctactacc agcatcaaca
     1561 agctaatgac gccaaaaaac agccaattgc tttggaatgg aaccgcatca ggtagaacaa
     1621 gggatgttcc cactgttcct aatagaccaa gaagaatacc aacaaatgat aaaatgaatg
     1681 tttgtgccac tacagaacgc gcaataaatc ctcctgtgat cccttgtgct ttcattacac
     1741 caaagatatt aatcttttgc atcgtcagta cataaatgaa aatcccgatc acaatagccg
     1801 caataacgat caaaaatccg atcataaatc caaaagtcaa tacttgcgca ttatatccag
     1861 gtaattcatt gatgaattta gaaattttga tctgttctaa atcttcaggt aaattactga
     1921 ttttacctct gacaactatt gcgttaatcc gtgcattttc acttgtatct tctttttcga
     1981 agcggatctc ttggtaagca ttgatggttg tatagagaac tggtgagaca ttgaatttgg
     2041 catgatccgt aaagccagtt agtttaaacg tcttgtcact accagataac ttaactgtat
     2101 ctcctacggc tagaccatat tcttctttca aactaatatc gcctactgcc tcatcatcgt
     2161 tatcaaaagc ttttccttca acaagattgg gcatgatgaa ttgattttta tcgataccga
     2221 agaagctaac attgattttt ccttcttctg tactgtcttt actagtcacg actccaggtg
     2281 tctgggcaag atacgccact ttcccacctt cgacttcttc tgctatcttc ttagtaatca
     2341 tggacatccc tagatttgaa tttgcatctt tcgaaagcac aatcgaatct gcctcccact
     2401 tgtcgactgc tgttcgatta tcttgagcta agccataagc taacccagtc aaaaaaaata
     2461 ccaggtaggc gatcaaaaac atcacccctg caactaacgc ataacgtaac tttgaatgca
     2521 taatttcatt taatgctaaa aacatttaac tcctcctcct gaaataatca ttttatacaa
     2581 caattttatc acttgataaa tttttggcga atattatgct caaaaaattc ttccgctatt
     2641 tcattatatg aattttttat tctcgattga ttaatattta ttatatcccc acacaataaa
     2701 agaccgtgat atttctcaca gtcttttatc tactctattg ctatttgctc tcttagctag
     2761 tactttaaaa gctaactcat tgaggtagtg aaaacgagag gtgtgttcag acagaaatcc
     2821 aaaatcctca actgctagct gtggaatcgt agttttgtgt ttcacgatat ccgcaagcag
     2881 gttcaaatct gataataaga attcttggct tatcagctgt ccgccaatta tttctaatgt
     2941 atccggatgg tagatcagct ttgcatttac tgttttgttt tcttcatcgt attgtggaaa
     3001 tagatcaaat tctcctgaac acgaaccggt attttgttcc aagaatggcg cttcatcttc
     3061 tgtcagccct acagttccta aaaagtagtc tataatgact gctgctgtta tcttttgtct
     3121 gaggtcgtaa gcggttggct gaccactcaa agttagcgct actgctcttg ccatatgtac
     3181 agctcgagta actaaaggta aaaagagatg ccgtttattt ttgtcaaaac tgactgggac
     3241 gatatctcca atcgcataga tgtccggatc acttgtctgc agatattcat tgactatcac
     3301 agtctcatcc tcatacaaag ccactttttc ttttaacaat gtcgtattag gacgagtttg
     3361 ggcagaaaaa atgaccatat ctgcctttat tgcttgagat agaagttgta cggatactac
     3421 tttctcttct gattttttcc aatcgatcaa gaattcattt ggataaaaat tgatttgctt
     3481 agtttgcagc tgattttgaa aactgttgat catatcctta tcgaaatatc ttgataaaac
     3541 attcggcata taatcaacta gatgaataat gtatccttta tttctcaaag catctgctaa
     3601 ttccaatcca atataaccag caccgaagat cactacttct tttgcttctt ttaaagcggc
     3661 aagtacttgc ttagattgca ctagattttt ataggtgtag acattttcag cttcaatttg
     3721 tttatacaag gtgacaggag aggaacctgt tgctacgatt aatttatcat aggttatggt
     3781 ctcgtttttt ccttctttgg atgcgaggat gagctgatgt ttgtctggat caagttcagt
     3841 tactctccat tcggtgatca gttttgcccc actttcttga agagtccgca tgttatgggc
     3901 aacttcagct aaactacgta tctttccttt taaataaagg ttgattccat ttgaaacata
     3961 gcctagtcca tctttatgat cttcatcgat cagtaaaact tctgctttcg gttgaagttt
     4021 tcttagattt agggcagctg tgatgcctgc atgggaagca ccaatgatca cgattttcat
     4081 gttttattca tccttaaatt caatttttct aaataacatt cttttaaaaa ttggttcaaa
     4141 taggggaaat cataattata ttcgttgcta gtgaatacaa aaactttagc ggacttctgt
     4201 gacgggtact ctttcaattg gctagtcaaa atgatatcat actgcttttc tttctcgtat
     4261 tcgactaaat catatttgat cgtattcagc aattgttcct ggatgatttc tttcacgata
     4321 tccgccatta gcctttcata aggaagatca taagcaattt gcagacgtaa ttctaactgc
     4381 ttgatcagcg tccgcaaaat agatgcatag cgagcaaata attcaaagcg ggcattttcc
     4441 tcgtctactt cagtaaaatc aacaatagca aatgtttgtc tgaccaatct ttcacataaa
     4501 accaggaatt gtttatcttc tacttcagtt aaccgctgtt ttaatccttc ttggccaaaa
     4561 atattcatcc atcctctaaa ataggcaaaa tgagcgtgat tttgcatcac tgtcaacgca
     4621 atcttttgcg aaatctcttc tggaaaaaca taaaaagaga aatattcttc tatcgattgt
     4681 ttaaacgttc gattcaattg agctactacg tcgtctttgt atttttcgct tatcaaaaac
     4741 tcctggacat gcgagttatg tgtatctaat gtaaagttac tgatcatgaa aagataaaaa
     4801 accaacgttt cattttgatt ccaaggaaag gcatattgaa agaagtaacg ggtcaaaaat
     4861 gtatggaggt ccaataaaaa ttgtgtcgtt tgaaacaatc caacaggaag attgctataa
     4921 tctggtttgg attttttcgt acagcgcatt cgcttcttca taatggaaag ccatatttca
     4981 aatttcaatc gtccttcttc tgtaatagta aaactaaact ctttttgcag cagatctaaa
     5041 aaagtgtttc ctgataaatc actgtattct tttttgtttt caagataagg acgtccaaac
     5101 caaaataatt ggaaataaaa ataacaaatt tgactttctg gaccggtcag ccgcccgctt
     5161 tttattttca gatgaaactc atttaaagaa tcatttaatt ccttcactct gcgagacaaa
     5221 gtagccgaac tgatattaag ttccatgaca agtttctgcc gatcaaatga gccattttcc
     5281 aacagataaa gaagtatctg atattttgta gaatagcgca agtagcaatg ataataaaca
     5341 tcgactgctg ctatatttcc atgtctgatt aataaaatat tcccttcttg gataacaatt
     5401 tccaattgtt tgatattttc caagttagct tgtaaataaa acagatcttc tttcaaggta
     5461 gcccgatcaa ctgataagcg ggtaagtacg ttattaacgg aaatctctcc tttttcctct
     5521 aataaaagtt ggaaaagttt gatttgccgt tgttcatttt tttcaagcaa actgaaaagt
     5581 tccaagtcca ctcctccaag catttctgat tttaacagca gaagtttcct tctcatcctt
     5641 gaaaaagagg atctgtgaca gttatcacag atccatacaa attctaatca cttagctggt
     5701 tggcccagct gtcagttccc atgtgaccat gccttgatac gttgcgccag gttgagcatt
     5761 cgtactgcta tttacctcta agagtaaacc atcggtatcg ggaaagtcaa cgagaacctc
     5821 tcctttagct gttccatcac tctcataaat caacgtgttt tgaccttgct gaatcaattg
     5881 ttttgatcca gaatgattga agtataattt tgttccgctt aattcttgac cttgatcatt
     5941 cttaaataaa gaagtcaatg tccctgtcag ttgccaagga gacgtgcttg gaagccgagt
     6001 atcccgcaca tcaaaagaaa aatcgccttt tgcttcataa taacttgtct tcaacggttt
     6061 ggggagatcg ttaccaaatg taaaatcatc tggtacatta actaatgtta attcttgttt
     6121 ggggacaaga attgttttag cagatgtttg gacaactggg ttaggaagct tagcaccatc
     6181 tgctaatgta atattgctgg aaagtacagc tgcctttgtc agtatgctga cggattgtac
     6241 agaagtatct acagtattta attgatagtt gtaagtaaga tgaatggtat ctcctatttt
     6301 gctcaataga tctgctggga cagaaactcc ttgttgaatg ccggtatctg cgatagttgt
     6361 accatttaat tttaaggaac cattgatata agtagtattt tcaggaagtg agatcgtgat
     6421 ttttttgtcg gttatcgcat ccccagattt tgttaacgtg aatgtcacat tttcttgaat
     6481 cgtatctcct gatagagcag acaggggatc tcctgtctca ggttgtctgc taaggttttt
     6541 tagctgacta tctgcactca cagatggaaa attagccact gttatttgag gaagagtggt
     6601 gattccagct tgttcagact gccatttgta cgcaaaagac ggatacatgt tagtaagtac
     6661 atcgtccacc aagcgataat caaaataata ttgccctgaa gccaatgcag gtagtgtaga
     6721 agtagctgta tttacacccc cgttatttga ttggatcgtt tggaaaagtt gatctgtcca
     6781 tcctgtcgct aatttttggg ttcctttcga ataaagcctg taatggatcg aacccttttt
     6841 tagttcagtt gctgaccaat tggccgtgac ttggtacgat gcagaagaaa ggagctttcc
     6901 ccactgacta tcggctgtta agtttgttgg taaaaccgtt ccgtctagct tgtactgctc
     6961 caataccttt aaagcaacag gagaaagcaa tccgccttgc gcatctttcc cactgtacac
     7021 gatattggct gtctgattca atccacttgt gcttgctgga gtatgagtca gtattgctcc
     7081 ttgtgtaacg ttttttcctg taaaatagtt gactacgcga tcaaagcgac taaatggcaa
     7141 ctgatcaaaa cgtccttcag tcagccccat cgtcaatgtc cgactattgg tagagcctgg
     7201 aaggttctgg ttatttccat ccacgtattc tgtggtatca gcaaatgtat tatattttcc
     7261 tgttgaatca cttgttgaga tgtaactatt ccacgtattt atctctgcac ttgcagaaaa
     7321 tgcatatgct cctggattca agtttccgcc agttaattta ggataaaatc tacctgcagc
     7381 tggagctaaa atcgaattga acatacgatc ttggataata ttattcgaat cataaaaatc
     7441 aacagaaaaa acggcctttt tagacataga aggttgtaat ttacccttgg tcaaaatttt
     7501 gtttggactc gtatcatatt tatctccgtt ttctgtcatc ttcgcgacat tagctgtaac
     7561 atcaaattga agcgggatat cgtccacgtt gaattcgcct cgcgtaataa tagcaataat
     7621 atctgccaat agagtatttg tatcccacga ccccatccca aaacgcaagg tatggcttcc
     7681 tgctgataat aaactaccgc tagtaacctt gatgggggca ccggcaatat acaagtaggc
     7741 gttgttccag tcaattgctt ttaatacatc actgcttgat acatgttccg gcaaattgat
     7801 gatcgccaca aatgcaaatc cttgtgcagt gatattcacc aaatcaagta catttgttag
     7861 tttcacatgg ctttcccaat gaaaacggac cgtgccacct ttccaaggat catttgaacc
     7921 atcgttcaaa taagtagcag tcaatttagg caattggtta gccccagtta gttggacaag
     7981 cttcccgcca ttgactgcac tacgtccacc atttggaggt acgatatttt catacgtgta
     8041 tggatcataa gaaatggcag gaggcgttgc tccaacagct tctgccgaag gtgaaaagat
     8101 aaatatactt aaaacaaaaa gcgaagtaaa aaagaaggag cgaaaaaatg aaaatttatt
     8161 ttgtagcaac atgttcatcc tccttttcct ttcttctcca taacaacaac cacgcaagaa
     8221 gccataattc tataccaatg atcaaataaa gaaaacggat cttttcttca ttcgtctccg
     8281 ggaatacgcc atgttgatta gattctttat aaatcggtag ctgtgtctgc tgttttgatg
     8341 cgccattgac tgaaccactt ggatgcggaa ctggacttag atcataaaat gtaatcgtac
     8401 ctgaactttt ggtatctgct tggactggct cttgccagct gaagaacagc aaacttgcaa
     8461 taatccctcc caataagatc gatgttttca acgaaatcac ctcatcatgt tgtttctcct
     8521 tttttctttt tcttgtggac ccacaaaaac agcaaaatca accataacac tagaactgca
     8581 gcaaccaatc ctgcataaag ccatgggaaa gattgttctt gtactgcaaa aacggaattt
     8641 tcatttactt tttgtgcttt ttcttttttg atcgtaaagt tttgtgtaaa tcgccattca
     8701 ttactgtcgc ctgattttgc tatgatttct gcgcggtaat cgccggcttt tgctttatca
     8761 ccagctaaat caagattcaa gtgaaaattc gaatttggtg caactaaaaa ttcattagac
     8821 gtattttcaa tataaagctg attttcataa aagatcttgc tcgtaaatat caaattgctg
     8881 ataatcctag gctgaggatt ttggaaacga agctgaacag ttggctttgc gttgacctcg
     8941 tccaaatctc ctgataaaag tttcagatcc ggttctatcg tctcttgact ttcttgtaaa
     9001 acgactgcga gattataagc aatacgattt ttgatttgtg ctccttctgt agcattttct
     9061 gtttcagctt cttctttagg tgaaacatga atacccgcta atacacgtcc cttaaagctt
     9121 tttgctggga ttttcacttc tataggtata gttgtttctg catgtgctga tacattgatt
     9181 tcctttttcg caatgagcaa aacgtcacgg acatcaaacg gcaatgtctc atctaacgtt
     9241 gggttttttt gactataatc gatcgttcca ccatcactcg ttaccgccgg attgacagag
     9301 acctcaaatg ttcgttcttc atctgaagaa ttgctgataa ttacttctag tgtctgattt
     9361 tgttccggtg ccactaataa atcaaaataa ccgattgctt tatctatttg gttctctgga
     9421 aataccggtt tgattccaaa gtctcctgct gaatcctttg cttggctgat tgacggaaaa
     9481 atgaaaaata gacttaaaat agaaaaaaac gaaacaaaca gtcgtttact ttgattcatt
     9541 gtccaccaca tcctttcaac aacaactgcg gtaaaaaatc cttcacctat agtattttca
     9601 ccgcagtttg ttgaatttat ttcatactct atgctactgg gccagccacg aggttccaag
     9661 tcaaatcagc cgtgaatgtt ccagctgctg ctttcggcac tttcagtgtg atatttgctg
     9721 gatcataaac agaaagccat gttcccagac cttgatcttt tgctgctgta aatattggtg
     9781 tcgctgcact tgctttactc aacccagtta cagtttgact agttggtcca gtggattcgt
     9841 tatctgacgt acttttaaca gtggatgttc caaaatctaa ttcagcgcct tgcaaaactt
     9901 gtgaaccgtt tacaaaatct gaaaccgaaa ctgtcacgtt ccacccttgt gcttggccat
     9961 ctgcacctac acctcgacga tctgatactt ggagataagg tgtatcgttt tttgaagcgt
    10021 ctacttgata cgtttttgtt ccgctttcaa tctcgtgtgt accaaatggc aattcaggaa
    10081 ctacatccaa tgttaaagaa cctgtctggc cagttgctgg attttccgga tctgctggct
    10141 ctactggttt tgaaggatca gtagggttaa gaggctcaac ggtatttgtt ggttcagtaa
    10201 aggtaatatc tccttttgaa gtcgcttgac cctcagcaaa agctggtgtt gccaagccgc
    10261 ctagaagaag tgttgaaagt aataatgtcc caatcatgtt ctttttcata ataaatcact
    10321 cctttttctt agtgacctaa gtatagtaga gacagtatgc aaaaaatcct catgaattca
    10381 tccgcttact tgaaatt
//
LOCUS       DAXWOO010000098        10364 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.098, whole genome shotgun sequence.
ACCESSION   DAXWOO010000098 DAXWOO010000000
VERSION     DAXWOO010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.098"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            6..305
                     /locus_tag="ACGE70_001917"
     CDS             6..305
                     /locus_tag="ACGE70_001917"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFF8693544.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACGE70_001918"
     CDS             378..1739
                     /locus_tag="ACGE70_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFF8693545.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACGE70_001919"
     CDS             1761..2021
                     /locus_tag="ACGE70_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693546.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACGE70_001920"
     CDS             2035..2787
                     /locus_tag="ACGE70_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFF8693547.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACGE70_001921"
     CDS             complement(2881..3486)
                     /locus_tag="ACGE70_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFF8693548.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ACGE70_001922"
     CDS             complement(3539..4513)
                     /locus_tag="ACGE70_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFF8693549.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ACGE70_001923"
     CDS             complement(4891..5610)
                     /locus_tag="ACGE70_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFF8693550.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACGE70_001924"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACGE70_001924"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFF8693551.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ACGE70_001925"
     CDS             7161..8558
                     /locus_tag="ACGE70_001925"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFF8693552.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDFFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ACGE70_001926"
     CDS             8639..9079
                     /locus_tag="ACGE70_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFF8693553.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ACGE70_001927"
     CDS             9227..9553
                     /locus_tag="ACGE70_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFF8693554.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ACGE70_001928"
     CDS             9554..10303
                     /locus_tag="ACGE70_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFF8693555.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
BASE COUNT         3288 a         1828 c         2149 g         3099 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaaattta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag atttttttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacg
//
LOCUS       DAXWOO010000099        10341 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.099, whole genome shotgun sequence.
ACCESSION   DAXWOO010000099 DAXWOO010000000
VERSION     DAXWOO010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.099"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            151..300
                     /locus_tag="ACGE70_001929"
     CDS             151..300
                     /locus_tag="ACGE70_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFF8693556.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            311..1825
                     /gene="dltA"
                     /locus_tag="ACGE70_001930"
     CDS             311..1825
                     /gene="dltA"
                     /locus_tag="ACGE70_001930"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFF8693557.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1822..3027
                     /gene="dltB"
                     /locus_tag="ACGE70_001931"
     CDS             1822..3027
                     /gene="dltB"
                     /locus_tag="ACGE70_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFF8693558.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKLFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3072..3305
                     /gene="dltC"
                     /locus_tag="ACGE70_001932"
     CDS             3072..3305
                     /gene="dltC"
                     /locus_tag="ACGE70_001932"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFF8693559.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            3308..4576
                     /gene="dltD"
                     /locus_tag="ACGE70_001933"
     CDS             3308..4576
                     /gene="dltD"
                     /locus_tag="ACGE70_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFF8693560.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4717..5799
                     /locus_tag="ACGE70_001934"
     CDS             4717..5799
                     /locus_tag="ACGE70_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFF8693561.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5799..6602
                     /locus_tag="ACGE70_001935"
     CDS             5799..6602
                     /locus_tag="ACGE70_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFF8693562.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6672..7412
                     /locus_tag="ACGE70_001936"
     CDS             6672..7412
                     /locus_tag="ACGE70_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFF8693563.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7423..8427
                     /locus_tag="ACGE70_001937"
     CDS             7423..8427
                     /locus_tag="ACGE70_001937"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFF8693564.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8472..10316)
                     /locus_tag="ACGE70_001938"
     CDS             complement(8472..10316)
                     /locus_tag="ACGE70_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693565.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         3328 a         1706 c         2088 g         3219 t
ORIGIN      
        1 aaagaaaaaa gcagtgattt gatgtgctat ttttataaaa gctatgtgac aaaactgtta
       61 gtaaaaaaac ggtttctgta agctagacac atgtttcttt ttgtcaaaat tagttacact
      121 ttgtgtgagg ttatatgagg agggaatcca atgaacaaat ggcgttctaa tccaaaattg
      181 aattattggg gaaaattcat cattcgaaca attttttata gtgcagtact gctgttcctg
      241 atttatctgt atcactataa aaatattcaa ggaggaactt ttatctataa cgaattttag
      301 gaggaagagt atggaaatca aaacgattat tgaagcaatc gatgattggg gaataaatga
      361 acctgatcga gttgcttatc aggcggatga gacgcatact tttggtgaat tgaaacaagc
      421 atcagacgca cttgcttatt atttagaaaa aaaggtagaa ggcgatggac caattgtcgt
      481 ttttggcaat ttggagtttg aaatgattgt ctcatttcta ggggttgtca aatctggcca
      541 tgcctatttg ccgattgaag agcatacacc caaggagcgt atcctatcga tctttcgggt
      601 ggctaaacca tctatggtca tcagtatcgg ggactggata gaagatattc cttcggttcc
      661 tgtcataaca aaaaaagaat ttcaagaaat caagaagata ccagtaggat tttatcccag
      721 aaatagcgta aaaggtacag aaaattttta tattattttt acttctggta ctactggaga
      781 accaaaaggc gtacaaatca gtcatgataa tctagtcagc tttgttcaat ggacgatgga
      841 agactttggg atcaagcctg gtatgcattt cttagcccaa gcgccatttt cttttgattt
      901 atcggtattt tctatttatc ctgcattagt ttctggcgga atgctgaagc cgttaaataa
      961 agcagttgtt caagactttc gtcaattatt cgcgacattg ccagccttga agttgaatgt
     1021 gtgggtttct actccatcat ttatggatat ttgcttgatg gaacctacat tcaatggaga
     1081 aaatgtgccg gaattggaca tgtttttatt ttgcggagaa gaattgacga aaaaaactgc
     1141 agaaagtcta cttgatcgat tcccaaatgc acggatatat aatacttacg gtccaacaga
     1201 agcaactgta gctatttcaa gtattcagat tgaccagcat gtactagata gttatgaccg
     1261 actaccgatt ggctacgtaa aaaaagacac gaaagtttct attgtccaag aaggtaaacc
     1321 tgtttcaaaa ggagaaacgg gtgaaatcat tattgcagga ccaagcgtat caaaaggtta
     1381 cctgaacaat cccgaaaaga cagctgctgc gttcttttgt caaaatgact tagcaagtta
     1441 ccatacaggg gatgcaggta gactagatga atatgggctt ttattttatg aaggacgtat
     1501 ggatcagcag gtcaaattac atggataccg gattgaactc ggggatatcg aacattatct
     1561 gctccaagac aaccgaatca aacaagctgt cgtcgtacca aaataccaag gaacaaaagt
     1621 acagcagctg gttgcttttg tcgtattaga aaacaaaacg aaacagccgg attttcagtt
     1681 gactaaatct atcaaagaac aattactaga agttgtaatg gattatatga ttcctcagaa
     1741 gttccattat gtcgatctgc tgcctcagac agttaatgga aaaatcgatc gaaaaaaatt
     1801 aacagctgag gtgaatcctg aatgatgtta cctcatatga tcccttatgc agagccgttt
     1861 tatttttttc ttttagccat tgcgcttgta ccgatcgttc tatctttatt gatatgggaa
     1921 aaaagattac cagtttatca atcagcagtg acacttttct ttttatttgt cagctttgga
     1981 ggtaattatt ggcaacaggg agcggcgctg attgggtatg ttttgtggca gacgatactt
     2041 gtatggggct attttgttta tcggcaaagg aaaaatcaga caggatggtt ttttgccgcc
     2101 gttattttag cgataattcc actgttttta acgaaagtga ctcctttttt tgaacatgga
     2161 aaagcatcgt tacttggttt tttaggaatc tcttatttaa cctttaaatc tgtacaaatg
     2221 atcatggaaa taagagatgg catgatcaaa acatataagc catttcgtta tatccagttt
     2281 ctgctgttct ttccaacaat ctcttctgga ccaatcgatc gctataggag atttgaaaaa
     2341 gatcagctcc atccgcctgt tcgtgataaa tatgttgatc ttttgggaaa aggcgtccaa
     2401 aatatttttg tcggtttttt atataaattc attatcggtt attattttgg tcaagtactg
     2461 cttcctgtag tgggacgcat tgccttacag catggtggta tttcatgggc attagtaggt
     2521 tatatgtacg tgtacagtat gtatttgttt tttgattttg ctggctatag cttatttgca
     2581 gtcggtacaa gctacatgat gggatatcag acaccaatca atttcaataa acttttttta
     2641 tcatggaata tcaaagaatt ctggaatcgt tggcatatga cgctgtcctt ttggttccgt
     2701 gattatgtat atatgcgatt gatgttcttc atgatgaaga aaaaattttt taaaagcaag
     2761 atcgtaatgt caaatatcgg ttattttgct ttgtttcttc taatgggtat atggcatgga
     2821 ttgacgtggt actacatcgt atatggactg tatcatgctt gtctgatctg tctgacagat
     2881 gcatggatcc gatttaagaa gaagcataaa gagcagttgc cttctaacaa atttacgcat
     2941 ggcttagccg tatttttgac atttcaagca gtatgtttta gcttcctgat tttttcagga
     3001 tttttagata aacttttttt tagctaacga atagtgacat attaatttat ataacaaagg
     3061 agaaaaggaa aatggaagaa caaattttat caatcttaga agaaataaca ggaacagatg
     3121 aagtaagaaa aaatcgtgat attgatctat ttgatgaagg gttgcttgat tctttagcat
     3181 ctgttcaatt attagtcgag ctagacggag agctggatat ccaagtccct gtatctgaat
     3241 tcgagcgaga ggattggagc acaccgaata aaatcattca acaagcaact gcattgaagg
     3301 gttgagtatg aaaaagaaac tttttgctat tttcggcccg gttgttttag ctgttggctt
     3361 actgtttgtg ttttttacct cctcctatca agtagatatc tctaatccag aaacgattag
     3421 acgtgcctct tcttccatgt cagtgaacgt gttgaaagga gatgcgataa aaaattatgc
     3481 attatctgaa aaacaatata ttcctttttt tggctcaagt gaattaagca gaatcagtcc
     3541 atttcatccg tctgttctgg cagaaaaata ccagcgaaac tatcgtccat ttttgctggg
     3601 agcacccggg acacaatcgt taagccaata tatgatgatg cgttctgcag gagatgctat
     3661 gaaaaacaag aaagtcgtct ttattatttc tcctcagtgg ttcgttaaaa atggtgtaaa
     3721 aactgattac tttaatacgt attattctga acttcaaaca tacgactggt tgttttcaat
     3781 gaagaaagta acgcctgctg atcgctattt agctagaaga ttattaactt tctcaaaagt
     3841 aaaagagaat gatacgttga cggcgattct gcaaacgatc aaaaaaggga agctaccttt
     3901 acctgaatct ttgaatcagc tgcgcagcca atggaacatg ctgaagagag aggatgaagt
     3961 attcagcaat attgggttga aagatcgaca gcaaaaaatc gatcatgagt caaaacgatt
     4021 accaaaacaa taccaagaaa cagaattatc cattttagca aatcaaattg gagaacgaga
     4081 aacgacgaat aatccattcg ggttgaaaaa cgatttttat acgcatcgta tccgagccca
     4141 cgagccagag ctcaaacagt cacaaaaaaa ttgggattat cgtttttcgc cagaattttc
     4201 tgattttcag ctggtcttgg atcaattagc aaaaaaccat aacgaagtat tgttcatcat
     4261 cccaccggtt aacgagaaat ggagcgatta taccggacta tctcaagaaa tgttacaagg
     4321 atttgccaaa aagatcaaat ttcaattaaa tagccaagga ttcaatcgaa tagctgattt
     4381 tgtcaatcaa gcgggtacca actattttat ggaagacacg atccatctag gctggaaagg
     4441 ctggttggct gcagatcaac agatcagacc gtttttagaa gaaaatcata ttactgctag
     4501 taagtatcat cttgacgatg ctttttttag caaatcttgg caacatcaaa taccagataa
     4561 actacaatta aaataaagaa aacaggcgag tgttgctcgc ttgttttctt tatttttgaa
     4621 tattctgaat aaaagataaa aataccaagt ttgcctgaac aaaaatcata agcaatggta
     4681 taatcacaat atgaatatag aaatggggga aaacatttgg atacaaaaga agaacagcta
     4741 cttattgatt taacttctgc aaaaggagta cctggaaatg aagaagaggt aagagaagta
     4801 ttcagagaat atgccaaacc attcgctgat gatatttttt atgacggttt aggcagtgtg
     4861 attgccaaac atggtgctgg cggaggcccc aaggtcttta tttctggaca tatggacgaa
     4921 gttggtttta tggtaacaaa aataaccgaa aaaggattct tggaattcca aactcttgga
     4981 ggttggtggg ggcaagtcat gcttgctcag caagttgaaa tcaaaacacg tgaagggaaa
     5041 atcgtccacg gtgtgattgg ttccaaacca ccacatgttt taacacctca agtaagaaat
     5101 aaaccttatg aaataaagga tatgttcatt gatattggcg catcaagcca agaagaagcc
     5161 aaagagtggg gcatacgtcc tggagatatg gtcactccat atattgaata caaacgaatg
     5221 aatggcagta aatatttact ggcaaaagca tgggataatc gtatcgggac agctgtttct
     5281 ctgcgagtat tagagaatct atctaaagaa gcacacccaa atgttttatt cgcaggaagc
     5341 gatgtgcaag aagaagtagg cttgcgaggt gctcgaacaa gtacacacct tgtaaatccc
     5401 gatattgctt ttgctttaga tactggtaca gcaggagaca ctccaggaat gacaccaaaa
     5461 gaagcagatt cgattttagg aaaaggccca cagattttga tttttgatgc atctatgatc
     5521 ccgcataaaa aattattgaa ttttgttatt gatgtagcag aggaaatgga tattccattc
     5581 caatatacag tgatttctgg cggaggaact gatgcaggac aaatgcattt gacaagagat
     5641 ggtgttccgt ctgtagccat taccgttcct gttcgttatc tgcattccca tacatcagtg
     5701 attcatgaag atgattattt gaatacagtg aaactagtca cagaattagt gaaacggctt
     5761 gatagtgaaa cagttgaaaa aattcggagt tatgaataat gaataataga atacctattt
     5821 ctgaatatcg gaaaaaagca cgagtttctt tggaaaatca atggggaatc aatacttggc
     5881 ttgttttctt atctataata tttacgagcg tcatccagaa cttttttgac ggtttcagtc
     5941 cttttccgga agaaagcatg cagcagcact tattgagttt tctagtcgat aatttacttt
     6001 tattcgcatt caactatgca ttgttttaca ttgcgctagt agttgtacgg ggtggcagac
     6061 caaaaagtaa ccttttattt tcagttttcc aaagaaagta ttttggttcg atacttggca
     6121 tcaatttgat caattcagtc atcaactggc tactcaacag tttaatcttt ctgcccatgt
     6181 ttttaatcag cggagtgagt acttatatgc agctgctttt cagttcaaat gcggtaccta
     6241 cgggtgtcat gccagaagat tttcttgata tcagctttgt tttatcgatg atagggatga
     6301 tttttgtttc agcactcatc atgagtattg ttggcggttt attccaattt gctgcatgga
     6361 caaagttcga ctatcctgat ttgtcggtta ttcaatgttt gaaatatggc tggtttttat
     6421 tgaaagatag aattggtacg tatattttgt tgcagctgtc ttttatcggc tggtatttgc
     6481 taggagcaat cgctttgttc atcggtcttt tgtgggtcat tgtttatgtg aatgtgtcag
     6541 ttgcagaatt ttatgaacaa gcacggattg aaaaaggagg tccagacgcc tattttactt
     6601 agcatgtaag cagtttattc atcaaaaaaa taacttttgt ggtaggataa atctttgagg
     6661 gggaatgtac gatgaaatta actgttttag gatgcttagg agcgtatcct tataaaaatc
     6721 aagggacaac aggttatttg cttcaatctg acgggttcaa tttgttgatc gatgcaggta
     6781 gtgctacgct gatcaaatta caagaacacc ttgatccact tgatctagat gctgtgatcc
     6841 ttagccatta tcatcatgat catattgctg accttggtgt attacagtat tactggcaac
     6901 ttcatcctaa gagaaatccg gagacattgc tttcaattta tggacacaca aaagattcca
     6961 ttcactttga agaattgacg ttggaaaatg tctcaaaagg acatccttat tttgaagctg
     7021 aagaattaaa aattggtcct tttctgatta cttttatgga aacaatccat ccagtagtat
     7081 gttacgccat gcgtatcgta gaagaaacaa cggggaaagt attcgtattt acaggtgatt
     7141 caggttattt ggaaagtttc gctgattttg cgaaagatgc tgatttgttt ctagcagata
     7201 cctatctttt tgaaggaaat gaacatcatc atgcccattt cacttcaaaa gaagctggag
     7261 aacttgcgaa atcagctaac gtaaagaaac ttgtacttac acacctacct cagtttggtg
     7321 atttagaaca attgaaaaat gaagcgcaaa aggcagcagg caaagggatt gatatagaac
     7381 ttgctgcagt tgacaaagtg tttaacattt aggaggaaga acatgatttt tgtaccaaat
     7441 gaaaaccacg acccaagaat caatttagca attgaacaat atttgctgca agaaatgcct
     7501 ttagatgaac ctattttgct cttttatatc aatgaacctt caatcatcat cggcagaaac
     7561 caaaatacgc ttgaagaaat caatcgagat tatgtggaag aaaatggcat tcatgttgtc
     7621 cgtcgcttaa gtggaggcgg agctgtctac catgattttg gaaacctgaa ctttagtttt
     7681 atcatgcctg atgatgggga ttcattccgt gactttgcga aagtaacaca gccaatcatc
     7741 caagcacttc atgaattggg tgttgctggg gcggaattga aaggccgaaa tgatctagtc
     7801 attgatggca tgaaattttc tggcaacgcg atgtatgcta ccaacggtcg gatgtttgct
     7861 catggaacaa ttatgttcga ctctgatatc aatgaagtag tcaatgcatt aaaagtaaaa
     7921 aaagacaaaa tcgaatcaaa aggaatcaaa tcgatccgtt ctcgtgtaac gaatatcaaa
     7981 ccatttcttc cagaagaaaa gcaaaaaatg acaacagaag aatttcgtca agaaatcttg
     8041 ttgaaaattt tcggcgtaga gtcagtagaa gaagtaaaaa cgtacgaatt gactgatgaa
     8101 gattggaaaa aaatcaatcg aatctctgag gaatattatc gaaattggga ttggaattac
     8161 gggaagtctc cagatttcaa tttctctcgc caaaaacgtt tttctatcgg ttcgattgaa
     8221 gtccatttga atgtctcaga aggaatgatc aaagaagcaa aaatctttgg cgatttcttc
     8281 ggactaggag atatctctga cgtggaaaaa aaattggttg gacagaaata tgataaagca
     8341 tctctatcaa aaatagtcag ccaaattgat gtgaagaaat attttggtgc gatagaacca
     8401 gaagaattgc ttaccttact ttattgaaaa taaaaaatag cggaatccac atatatggat
     8461 tccgctattt tttatacagg attcgggtaa tttcgagaga gttgaacagt gatccgatca
     8521 atgaaagtat ctttttcctg cgttagtgtt cctgtgtaac gatccgctac gtctttcatc
     8581 atcattaagt caaaacgttc ctgatcatct aattcttctg tgatgcctcg actaaatagg
     8641 tttgtatcgt aatgtgcact atgttcgcaa caaatgacga aatgttcctc ttgttcgtgt
     8701 atcgacaaat aaatttttcg atacagtgga ttttcgatgg catgagtttc tctaaaggaa
     8761 tgctctaaag cgtggatcaa aagttgagcc agatcgtcat cgttgatagg aagttcttcg
     8821 ggaagatctg cttggaaggt tatctgaata ttccgtttgg cagctaactt ctggtaacgc
     8881 gctaagataa gatttgtcac ctggtggttg caaaaagaac acagcatttc gaaatttttt
     8941 gcttcttcta atatcttttc cacatagttt ttagcttttg tttggtccgt atgcattaaa
     9001 tcttgcagat tctccatctc ttggacaaat tccaggcgaa gtgtatttac ttcttggata
     9061 tgctgtcgaa gctgctcata atgttgctcc aacaaatcat ttttgatttt taagaaatct
     9121 acttgtctcc gatctttgtc ggtatttaag atgtgatcga ttagattgta gctgataata
     9181 acgattagaa tgataatata taaaatcgta gaccaattga tcgttgtttg atagatacct
     9241 aatgccgttt ggatgtaaat caagcaatgg ataatggcta ccaagacgat ccaaggtgta
     9301 caagctacat aaaaacggtt attcttatat gcttcgccaa tgcatgcaag tgaagtgact
     9361 aaagtgctta taacactagc aatattagca atcggcatta attcaggaag ttctgcagga
     9421 gtaaaagttg cataaagcag aacgccgata aaaatcaaaa gttggaacgt acaaaaaatt
     9481 ccgtaccatt ttttatagta aaacattcgg ctccaatagt agcagatgat gaaagctgac
     9541 gttaagataa taaataaatg agataaaaaa gaatgaacga atggttcaaa aagaatacca
     9601 cttaaaatat cttctgatac agcttcaaat cctaatgcca aagcaaagca acttaagaga
     9661 ataagtgagt attcaagacg tttttgttta tacatcaagt agatcgtata gaaaaaggtc
     9721 cctatagcga taaaaacagc gattatcatc gtcaatactt gcggcactga ttgtgaataa
     9781 ataaaactaa tcaatggtcc agttgttcct agataaactt tcggtggcag tccagcatag
     9841 ttctgataag gagatgagac tgtaagggtc aatttttttc cgatataatt tgctggtaaa
     9901 tctatcacag ccaatgagag tccgggattg ctttcttcct gataatcttt ccgttggtat
     9961 aaaatctttt cgcctagata tacagtaatc cattgatggt ttcctttgat tagtaaattt
    10021 gcttcgtcca acggtttttc aagtgtcgtt tccataatga atgtttcgtc tttatccacg
    10081 gtaggaatcg tacgagtgta cttgctgtca aacttaaaat caggatcatc ttttgtatag
    10141 aaaacccacg aatcttttat ttctttcatt tgattgctgt ccgcaggaga aaaaataaaa
    10201 taaaagaaat aagcaattcc tgttaaaaat aacaatgata atataatgat tatgctccac
    10261 tttatacgta aataaaaaga ttctttttta ttatatatgt tattaaaaaa agacataaag
    10321 gtattccttt ctaaaacttg t
//
LOCUS       DAXWOO010000100        10066 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.101, whole genome shotgun sequence.
ACCESSION   DAXWOO010000100 DAXWOO010000000
VERSION     DAXWOO010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.101"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            9..911
                     /gene="gnd"
                     /locus_tag="ACGE70_001939"
     CDS             9..911
                     /gene="gnd"
                     /locus_tag="ACGE70_001939"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFF8693566.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            913..1914
                     /locus_tag="ACGE70_001940"
     CDS             913..1914
                     /locus_tag="ACGE70_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFF8693567.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALIERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            1911..2195
                     /locus_tag="ACGE70_001941"
     CDS             1911..2195
                     /locus_tag="ACGE70_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFF8693568.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            2228..3025
                     /locus_tag="ACGE70_001942"
     CDS             2228..3025
                     /locus_tag="ACGE70_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFF8693569.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            3039..3872
                     /locus_tag="ACGE70_001943"
     CDS             3039..3872
                     /locus_tag="ACGE70_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFF8693570.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            3890..4747
                     /locus_tag="ACGE70_001944"
     CDS             3890..4747
                     /locus_tag="ACGE70_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFF8693571.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            4769..5278
                     /locus_tag="ACGE70_001945"
     CDS             4769..5278
                     /locus_tag="ACGE70_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFF8693572.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            5315..5716
                     /locus_tag="ACGE70_001946"
     CDS             5315..5716
                     /locus_tag="ACGE70_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693573.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            5828..6181
                     /locus_tag="ACGE70_001947"
     CDS             5828..6181
                     /locus_tag="ACGE70_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693574.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            6215..6658
                     /locus_tag="ACGE70_001948"
     CDS             6215..6658
                     /locus_tag="ACGE70_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFF8693575.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            6675..7760
                     /gene="uxuA"
                     /locus_tag="ACGE70_001949"
     CDS             6675..7760
                     /gene="uxuA"
                     /locus_tag="ACGE70_001949"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFF8693576.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            7753..8466
                     /locus_tag="ACGE70_001950"
     CDS             7753..8466
                     /locus_tag="ACGE70_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFF8693577.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(8673..8822)
                     /locus_tag="ACGE70_001951"
     CDS             complement(8673..8822)
                     /locus_tag="ACGE70_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693578.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            9218..9418
                     /locus_tag="ACGE70_001952"
     CDS             9218..9418
                     /locus_tag="ACGE70_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFF8693579.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            complement(9841..>10066)
                     /locus_tag="ACGE70_001953"
     CDS             complement(9841..>10066)
                     /locus_tag="ACGE70_001953"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693580.1"
                     /translation="VFRRKLIISKYSLKKLITIMPFLIYNTVIFKNKNAKSLYNNGES
                     TKNKKIITLLKKQMKDQVLLDLILSSLEGI"
BASE COUNT         3396 a         1559 c         2210 g         2901 t
ORIGIN      
        1 gcgctatcat ggaaattggt gtaattggtt tgggaaaaat gggattgaat ttagctttaa
       61 acttaaaaga gcaccatcac aaagtacaag gactcgacat ttcaagtaca gctgttagtc
      121 aggcaagaga aaatgacatt cctgcttatc aagagatctc tgaatttcta gctacttttg
      181 agcagaaaaa aatcatctgg ttaatgcttc ctgctggaga gataaccgaa aacatgttaa
      241 gaaaactttt tccaaaactc ggaccaggag acattattat tgaaggcgga aactcttatt
      301 ataaagattc aatccgtcga gcagaagagt ttcagaaaaa tgaaatcggt tacttggatt
      361 gtgggacttc tggaggaatc gaaggagcac gacatcaagc ctgcttaatg attggaggag
      421 ataaagaaac ttttctatcc gtggaacaat tattcaaaga tgtagcaatt gaaagaggat
      481 atctgtatgc tggtgcatcc ggaagcggac acttcttgaa aatgatccat aatggaatcg
      541 aatatggaat gatgcaagca atcggtgaag gattccaact tgtacaaaaa agtgagtatg
      601 cttttgactt ggaacaagtc gcacgtgttt ggaatcatgg ttcggtgatt cgcggctggc
      661 tgatggaaat catggaagat cagttaaacg aacatcccaa tttaagtaat tatcggggaa
      721 tcgtttctgc atcaggtgaa gcaaaatgga cggttgaaac agcgctagaa atggctgttc
      781 cagttccagt aattgcaact agtttgttca tgaggaatct ttcacaggaa gaagatagtt
      841 tttcagcaaa agtagtttca gcactaagaa atgggtttgg cggacatgaa atagttgaaa
      901 agggtgagta aaatgccaga ttatcatata gcaatcgtta attccagcag tttcggaaga
      961 gtctttccac accacttgga aagattaaag tcagttggag acgttcattt ctttactgtt
     1021 gatcaagaga ttccaggaaa agaactagcg gagttactta aaggattcaa tatcattatt
     1081 gcaagtgtta cgccattttt tcgacgagat ttttttgaaa acaaagatga actgctattg
     1141 atttcccgac atggtattgg ttacaacaat attgatttaa aagcagcaaa agaacaccag
     1201 acaatcgtat ctatcatacc tgctttaatt gaacgaaatg ctgtagcaga aaataatata
     1261 actaatttgt tgtccatgct tcgacgcacg acacaagcac aagcaagagt caaggacgat
     1321 cgatgggaag atcgggcgga gttcgtcggt aggacgctgt ttaacaaaac agttggtgtg
     1381 attggtgcag gaaataccgg aagcggagta atcgaaattt tgcgtaacgg ttttcgctgt
     1441 gatgttctag cttatgatcc ttacaaatca gatttataca tcgaaagctt tggtgcaaaa
     1501 aaagtagaac ttcaagaatt actggaacaa tcagatatca tttgtttatg tgcgaatttg
     1561 acagaagaga actatcacat gatttctgat aaagaagtcg atcagatgaa agaaaatgtt
     1621 tatctatcta atagtgcaag aggcgctcta gttgatgaag aagcgatgat taatggtctg
     1681 aaaagcggaa agatcgcagg atatgccaca gatgttttag aagaagaacc gggacgtcaa
     1741 gatcatccgt atttagcgtt tgaacaagta gtgattacgc cacatatatc ggcctacaca
     1801 agggaatgtt tagaagcaat gggtgaaaaa tgtgtcagag atgtcgaaca gatcagtcag
     1861 caccttcttc ctgaacgttc tgtccagaca aagagcaact atataaaagg atgatgaaga
     1921 aaatggtgaa cggtttaaaa gtgaaaacag gccctcaatt ttatttatat gaagaaggtg
     1981 gtatttcaaa agtttctgat ctcttgaaat catacggcgc gaaaagagta ctggtcaccc
     2041 acggaacggt atcatgggaa aaggcgctac ctaaattagt atttttgaat gacgaaacga
     2101 ttcaattttt ttatcatcgt tattctggag aatgtagtta tgctgaagcc agacgaatag
     2161 ctacaatcat caaaaaaatg aaatcgattt cgtagttggc gttggcggag gaaaactgac
     2221 cgatctagtg ggttatagtg ccaatcttgc aagaatcgat ttcggagtca tccctacttt
     2281 agctagtaat tgtgctccat ggacacctct tgctgtcatg tataaggaga atggacaagc
     2341 agaagggaaa acagaacact tttacagaca agctgctttt tttataacag atccccaatt
     2401 agtgattgat tcccctgttc catatttcat tgccgggtta gcagatacac tagcaaaatg
     2461 gtatgaatct gagctcatct tagaacaaga ttttttgcaa gatgaatcat ttttgaagct
     2521 agcacaagat actgcgaaga tctgcaaaga agagattttg aatcattctg ctaaagcaat
     2581 ccaagatatg gcacaaagaa aactgacatc ggagttcaaa cagctttcag agattatctt
     2641 tgctgttgct ggtttagttg gaggattagg agataaatac gcaagaaatg cggcagcaca
     2701 tgctatgcat gacgctatca gtaaatatat tccggaaagc catcgttttc tacatggaga
     2761 aaaagtagcg tatgggattt tctatcaact ggcactagaa ggtcgctggg aaaaaataga
     2821 cgagttatat cctttgtatc gggaattaga actgccggtt tctctagaac aaatgggatt
     2881 atttccgaaa gataaacaga taatagataa aatggctgaa tttattgatt cgaaagagaa
     2941 agtgcatttg attcctatag aggtaacaca gaaatcactg atacagtcaa tgatggaact
     3001 ggaaggttat atcagaaaga tctgaaggag gaggacacat ggaaacatta agtgtagtac
     3061 aaagtttgtt gattgcttta tgggtagccg cggttatgtc ccgctggtta ggtggaggag
     3121 ccacgttgac cttacgtttt tctcctttga tgactggatt agtcgtcggg ttgatcatgg
     3181 gggaagttgc acaagcgatg attgttacag ccgctttgca aatgatttat atgggggttt
     3241 tttctccagg aggatcaatg ccagctgaac catctattgc agcagctatc gcagtaccag
     3301 tcgcattatt aggggatctg tcgccagaag cagcgattgc cgttgcggtg ccagtcggat
     3361 tgctcggaag ttacttgtat cagtttcgtt ttttcatcaa tactttttta ggtaaatata
     3421 cagacaaagc tgtagaagat ttgaatacaa aagctatggc ccgatcaatt attctttatc
     3481 caacggttgc ctcattttta ttattcgttc cgcttgtttt cattgcttta tatttagggg
     3541 cgccattgat cgctgatgta atcaaagctt tagagggaac agtcgttatc cacattcttg
     3601 aagtagtagg tggagggtta gcggcaatcg gtatcgcgac aactgtatac gttatcggtc
     3661 gaaaagatta tctggtcttt ttcttccttg cttattttat gagtgttgtt ttgaaatctt
     3721 tagatattac gatggttact tatgccattt ttgggattat tatcgctttg atctttgtcc
     3781 agtcgcaaaa agggaaacaa gtacagcctg ccacaagcaa taacaattca gcatcttttg
     3841 atgacgaaga tgactatgat gacggatttt aatttaggag gaatgggaaa tggctgaaat
     3901 aatggataat caaacaggtg ctcctgaaga aatcacgaaa aaagatgtaa caaaagccta
     3961 tctgagatgg cattttgcaa acgaaattcc tcactctttt gaacgttact tggcgccatc
     4021 actactatac ggaatgatgc caatcttgaa aaaactttat aaagatgaag actctttaaa
     4081 agcagcatat aaacggcagt tactgttctt caacactcaa ttaagctggg gtggcggtgt
     4141 gattactggc ctaatgtctt ctatggaaca gcaaagagca gaagaagagt atgaagaaaa
     4201 agagatcttg atgcaggatg acttgatgta taacacgaaa gcgggattga tgggggcact
     4261 agcaggaatc ggagatgcaa tcgattcagg aaccgtccaa tatattttta tcgcaatcgc
     4321 ggtaccttgg gcacaacagg gaagtccttt aggcgcactt tttcctttta tctgctttgg
     4381 gttgtatcag gttttactag gggtattttt cgcaagacaa gctttcacta tgggccgtaa
     4441 tgctacaggc ctaatgcaaa acactggtgt tcagacagcg attgaaatgc tatcagtttt
     4501 agggttgttt atgatggggg tgcttgcggg aaactatgta aaagtagaat cttcattgaa
     4561 atttgcgata tccggacgtg agtttgttat ccaggaaatt cttgatcaaa tcgtgccagg
     4621 gatattacct ttagctgtcg taatgggtgt gtacttgttc tatacgaaaa aaggattgaa
     4681 agtcacacaa gcattattat ggctgactgg aatattaatt gtgctagcag gaatcggtat
     4741 tttataaaaa gaagggaacg tgaataagat gggtgtagtc aatttagcgc gtgtggatga
     4801 gcgtttgatc catgggcaag taatggtaac gttatctcaa aaaagtggag tgaattccat
     4861 ttttgttgta gacgaagttg tagcaaaaga taaatttatg agagatcttt acaaaaatgc
     4921 aggaagccgt acaggacaaa aaacaattgt cattacacca gagaaagcta aattctattg
     4981 ggatgaatat caatttaaag aatacaattg tattttgatc gcaaaaacag tccaaacgat
     5041 ttacgactta gtaaaacacg gaatccccat ggaagaattg aatattggag ggattgctca
     5101 aaaaaatcct gatacagatc ttttagtgac gaaatctgtt tatttaaata aatcagatgc
     5161 tgaaaaattg aaagaaatga atgaacaata tggcgtaaag aatatttatt ttcaagccac
     5221 tccatctgca cctaaaacag gattaaaaga tgtattgaac aagtttgatt tgtcataaac
     5281 aaatgcttag aaaaaagata aagagggcga aaaaatgaag aagaacgagc tgtttcgtga
     5341 ttgggaattt cgttatcgct atatttaccg taagcgccgt acaaaaaaga gtaaacagcg
     5401 ttttctttca gcattagtat cagatattta cagtatgaga acagatgtga cagtcattgc
     5461 ttacgatacg ctagcctacc gatcaaaaaa tatttatgtt ggagatatcg aaaaagcaga
     5521 gaaagttatc tgtacttatt acgatacacc cgttcatgca ttaggaagtt atttcatgtt
     5581 tgattggaaa gaccaaagga aaaaaacaat ctactctata ttattgtcct tcattttgtt
     5641 gttcagttta ggttggtggg gaatgatgat atataacaaa aatcctcatc acgtattcga
     5701 tctgctatcc gtataaacag gtatcactgt actagcattt gggagctact ttttcttatt
     5761 gggaaaagca gctcgcggat ggcctagcag acaaactttt attcgaaata cttcatccat
     5821 attaacgatg ctagaaatga tccgtacgat tgatgatccg agtgtagcgt atgcgtttgt
     5881 tgatgaaggc tgttatggag aaaagggctt agacagcgtg agatcgggta tgaaaaaaga
     5941 ggctattctt ttttatctag actctgttgg ggcagataca ccgctacagt tttcgggaaa
     6001 ttatttttca aataaagaac agtggctgaa acaagttgat aagttgaaag aaaaaaacgt
     6061 caattatatt tttagtgcaa gaaaaaagca agcgcagttc ttctatttga ccaaaacaga
     6121 tttacgaggg aaaacattta attggcaaaa tgccaatcag attattgctc tttttcgata
     6181 agtaaaaagt gcaaaacaag gagtgagaag actaatgttg gaaatcatga tcgttacaca
     6241 tggtaagttg agcgaaggac tgaaagatgc ggcggaagta attgtcggga catcgagcca
     6301 tattgaaaca atgtgcttag agtctgggga ggacatccaa acattcggag agaaggtcaa
     6361 agaaaaatta actatgatca acacagcaga acccatgcta gtatttgttg atctaattag
     6421 cgcaagtccg tacaatcaag cgctcttggc agtcaatgaa ctgactgaag aaaagaaagc
     6481 agccacttat gtcatagggg gcgtgaatct gccaatgttg ttagaagcaa tcaatcatca
     6541 gatgcttcac acaccgatag aagaagcggt ggaacaaatt gtcaaacaag gaaaagataa
     6601 tttaactgtt tggcatgctt cagcggaaga aaaaaaatta gatgaggacg atttttagaa
     6661 ggaaggctga aaagatgaag tggggattta gatggtatgg tgaaaaaggg gattcgatcc
     6721 cattaaaata tgttcgccaa ataccgggaa taaatgggat aattggaact ttgctgacta
     6781 aaatgcctgg agatgtttgg gagatttcag aaattgagga gttgaaagca tctgttgaga
     6841 gccaaggact ggaactgctt ggaattgaaa gtgtagcgat ccacgatgca atcaaggcag
     6901 ggacagatga acgagattac tatattgata actatatacg aacaatcaaa aacttgtctc
     6961 gatgtggggt tcgattgatt tgttatagct tcaaaccaat ttttggatgg gcgaaaactg
     7021 atttgtttaa tgaaaacaac gatggaagct attctctttt atacgatcag aaaatcgtcg
     7081 atcagatgga gccaggagaa atgtatcaat tgatacacag ccagtcaaag ggattcaaat
     7141 tgccgggttg ggaagaagaa cggttgaaaa aattcgctca gctggaaaaa ctgtatgaag
     7201 gaatgaccga agaagaatta tttgaaaatc tgacttattt tttagaaaaa gtgattccag
     7261 tgtgcgaaaa agcagatgta aaaatggcta tccatccaga tgatccgcca tgggaacttt
     7321 ttggttttcc tagagtgacg aaaaatcttg gcgacttgaa gaaaattcta aatgcagtag
     7381 attctccata taatggacta acgctgtgca caggatcttt aggggcaaat ccggaaaacg
     7441 atttagttga aatcatccat gaggtaggtt cgcgcatcca ttttgtccac ttcagaaacg
     7501 tggagttttt aggagaaaga aagttcaaag aaacggcaca tccctctaca gaaggctccc
     7561 ttgatatgta tcatattatg aaagcgttgg tgcaagaagg gttcgacggt cctatacgcc
     7621 ctgatcatgg tagaaccgtg tggggtgaag tggctatgcc tggatatggc ctgtatgatc
     7681 gtgcaatggg actcacttac atgcaaggtc tatatgaggc gataagtaag gaaaaaccac
     7741 tgggagagca ttatgaataa aatcaagctc ttcatctttg atatggatgg ccttttatta
     7801 gaaactggac gtttagctta tcgtgcttat gtaaaatcag cacaaaagta tgattatgaa
     7861 atgcgtaagg aagtctatta tttactaact ggccaaacag aaatggcgat aagagaacaa
     7921 atgggcattt tgtacggaga agatgttcca tatatcaaat ggcgagaagc aatcaatcaa
     7981 tataaagaaa aaatcgtgaa agaagataag agagtatata cgaaaaaagg agcagaagaa
     8041 attttatctt ttgcaaaaga aagaggaatc catacaatcg tggcttcgtc taatacacgg
     8101 gtaaaagtag aaatgtattt aaaaatggaa aatctatatg ccttgtttga tcaaatcatt
     8161 tcaggggatg atgtaaaaaa aggaaagccg gaaccagaga tctttttgaa agcttgttca
     8221 aaaatgaata ttcctccaag tgaagcactt gtatttgaag actcgattgc agggatcgaa
     8281 gcagcaaggc gagcgggcat tttgtctttt ctcatcaaag atcatttgat agggttgccc
     8341 gatcataatg ggaagcataa gttgaaagta gatgtaagtt tatatgaatc caataaagca
     8401 gactatgaat tttatgacct gcatcaagca aaacgatttt tagaaagtaa agggttgtat
     8461 ctctaataaa gaggactatt ttctagtcct ctttatttat tgtcctgcac gactgaggga
     8521 acagatgaaa aagaagtaat tttttatttt gaagtatcta ccttatttgt agtgaacaat
     8581 ttagaacaaa cacaaaacgc atctactgtt atctaccagt agatgcgttt aaagagaaga
     8641 acgagaatcg tccatatgag ggtttccact ccttatgcag atttttttaa ttcttgcact
     8701 gcttgttcga tcgtaacacc tgtcgctaca tgattggtat tttctttgtc aaaaacgaca
     8761 aatagattca aatctgtatc caaggttact cgatattgta tatctttgtt atagaatgtc
     8821 atagccattc tcctttcttt aaatacttgc tgaaaggcaa cgaacgcgtt gataacgaaa
     8881 ttccatttat aagtatacca ccaattgaaa ggttttcata gttaagaaat tttaaagtca
     8941 aaagttgcag ataaaagcaa ataaacaaga aaacaaaaag tgaatcaatc gcggttattc
     9001 aactttttct tcgattcgaa gacaatagaa gacaatgaat tttatatttc attgcattat
     9061 ccttgtgtta ttagaaaaag catgatatat taaatgagta atctattttg aaacgtaatt
     9121 tgagcgatat atctacatag ataaaactcc ttttgcctat caaatattta ttgcaacgaa
     9181 aaaacgtaca ttgatacgta tcaggaggaa tttatttatg aataacggta cagtaaaatg
     9241 gttcaacgca gacaaaggtt ttggctttat cactggtgaa gatggaaatg atgtatttgc
     9301 acatttttca gctatccaag gtgacggctt caaaacttta gaagaaggcc aagcagtgtc
     9361 ttacgatgtt gaagatggtc aacgtggccc tcaagcaaca aacattgtta aagcttaata
     9421 aaaattgact tttaaccgtc ttttcgaaag aaaagacggt ttttttgtac gaaaaaattc
     9481 attggtcgtt gaatgggctg aatagtagtg tataaaacaa ttttgtggag cgtgaagctt
     9541 ttatggtaga ggaaaatacg aataaagtga tgttgataaa aagattccta tcttgaatat
     9601 gaatcgagat gtcttgaaaa tgaatgagtt tgaccaatca ttatcctatc gaaatttaca
     9661 gtttcgttcg attggtctgt caattaatga aaaagtaaaa aagaatatgt tctaggaaga
     9721 gggcggcaca tctaaatttt taagtaacgg acagttcaaa tatcaagcat tgaaagaggc
     9781 tatcctactg tttgaaggaa tacgaatata taaaaacgac aaagaggaaa aagtagttca
     9841 ttaaatccct tcaagtgatg aaagaatgag atctaataaa acctgatctt tcatctgttt
     9901 ttttaataac gtaataattt ttttattttt tgtgctttcg ccgttattgt agagtgattt
     9961 agcattctta tttttaaaaa taactgtgtt atatattaaa aaaggcataa tagttataag
    10021 cttttttaaa gaatatttag aaattattaa ttttcgtctg aaaaca
//
LOCUS       DAXWOO010000101        10042 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.102, whole genome shotgun sequence.
ACCESSION   DAXWOO010000101 DAXWOO010000000
VERSION     DAXWOO010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10042
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.102"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(505..729)
                     /locus_tag="ACGE70_001954"
     CDS             complement(505..729)
                     /locus_tag="ACGE70_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693581.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            920..3697
                     /locus_tag="ACGE70_001955"
     CDS             920..3697
                     /locus_tag="ACGE70_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFF8693582.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            3778..4197
                     /locus_tag="ACGE70_001956"
     CDS             3778..4197
                     /locus_tag="ACGE70_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFF8693583.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4209..4679
                     /locus_tag="ACGE70_001957"
     CDS             4209..4679
                     /locus_tag="ACGE70_001957"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFF8693584.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4696..5475
                     /locus_tag="ACGE70_001958"
     CDS             4696..5475
                     /locus_tag="ACGE70_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFF8693585.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5462..6283
                     /locus_tag="ACGE70_001959"
     CDS             5462..6283
                     /locus_tag="ACGE70_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFF8693586.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6300..7349
                     /locus_tag="ACGE70_001960"
     CDS             6300..7349
                     /locus_tag="ACGE70_001960"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFF8693587.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7362..8366
                     /locus_tag="ACGE70_001961"
     CDS             7362..8366
                     /locus_tag="ACGE70_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFF8693588.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            8999..10018
                     /locus_tag="ACGE70_001962"
     CDS             8999..10018
                     /locus_tag="ACGE70_001962"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFF8693589.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3365 a         1657 c         2094 g         2926 t
ORIGIN      
        1 actctattta cttctttttt aatttgttct tgcaatcttt tttgctacgt aatttttgta
       61 aaaaagtggg ttgagagaaa tctaattgat tttcaaagaa ggagtatgaa aatgagaaaa
      121 atatttattc gtttattgtg ggtactatct tagaggaata aataatagtg gagaaaacta
      181 tggtttggcg cagattctat caggtttgat ggaacgaaga caacctcccg acatcaagtc
      241 aaaagctact tgagattttg atctgaaaat aaaaaaagaa aacaaggtag gaacatcaat
      301 gtttctacct tgttttcgta atcaatggga aaaaactaag agtggtgtac gtaatcaata
      361 aaaaggatgg tgacaacagt acctctttac tctctgaaag catcataact cgtttttcct
      421 cattatgcca ataaattgtt gttataccac atataaggat ttgtaatagc taaaaacaga
      481 caggaaagtt gaaatctgtg aatcttattt tgtatggaag gatttataga acgcaaaaat
      541 caagataaag atgaataaaa atcctgtttc ggctatatat tgcgtatcta cgccaataaa
      601 acgagtttgt tctgtcatag ccatataaag caaaggcaaa aatacaacta cagcgagata
      661 agatccgatg atttctcgaa aagttttcca ggaagtatgt ttcctgtttt tatatttatc
      721 cgatttcatt gaatagtcat agcctcctta ttctttcata tgttgaaatt ccagtataaa
      781 aactggaagg aactgtacgt tttttagaat aaaaattata agggataagt aggctgctgt
      841 ctataataag atatattttt caattcttta ttaaagattt tattgacaaa atgaatccgt
      901 tttctatgat ggaggagggg tgaataagtt gttgaagaag gaaattttat gttacttaga
      961 gaaccagaca gcctttttcg atttagaaca tatgaacgag gtctttacag cacaaaggtt
     1021 agctgatcat tttggggtaa aaagaaacac agtcagtcat tatttgaacc agttaaatga
     1081 tgagcaacta ctagtcaaaa tcaattctcg accagtgatt tatttccaca aagaagcttt
     1141 tgaaaaacaa ttttttaagt tacgcacgaa tttttatttt tctatcgatg atctcaaaga
     1201 ggaacagcct tttttcgcgc aaccaaaaga cttattctct tttatgatcg gatacgacgc
     1261 cagtttgaaa gaaagtatcg aacaaatcaa aacagctctt tactatccag atggcggatt
     1321 accacttttg attacaggcg aaagtggcac aggtaaaagt tatttagtta atttggtcta
     1381 tcagtattgt ctgcttcatg atctgctaga agattctgca ccattcatca cttttaattg
     1441 tgcacaatat gcagataatc ctgaactttt gactagcaat ttgtttggac atgttaaggg
     1501 agcgtacact ggtgcagaag aaaataaaaa aggggctttt gaagcagcag atggtgggat
     1561 cttgttttta gacgaggtac atcgcttaac accagaaggt caggaaaaac tgttcaccta
     1621 tcttgatcaa ggtgtgattt atcgtatggg agaacctaat ttgtcacgcc ggatcaaaac
     1681 acgtctcttt tttgctacga ctgaagagat tactagtcat ttcttaacaa ctttcatccg
     1741 aagaatacct atccgaatcg aactacctgc acttaatcag cgtagtcgta atgaacggct
     1801 agaactgatt tactcttttt ttctcgaaga acaaagaaaa atggggagcc ctttgtcagt
     1861 cagtggacca gctttgtcac ttttaacaag tcagaaatta tccggaaaca taggagagct
     1921 gaaaaatatc gtcaaagttt cagcagcaaa agcatatgct gaacagcgtg aacagcctga
     1981 aatccatgtc acgatccatc atttgcaaaa agaactactt gcgcagccta ttactaaaaa
     2041 tacagcacag caaggaatct atattacgca agaccagacc ttagagcaat taatggagaa
     2101 gcaacagccc gaacagcagc gaatcgttaa aagttttgag caaatcctaa ttgatttcaa
     2161 gaaaaataac tgtctgctat attcttgcga aggaaaatta aagcaagaag tgattcaatt
     2221 atttgatttt ttgctgtttg agaccagtag acaagaaaaa cacgagctgc tggtttattg
     2281 gacacaatct atccgagaga cctttcgaca aatggaaact gcctatcaaa tcaaattcga
     2341 tggaaacagt gtctatgcct tgagttacta tctctatcag cgaagcacta tcaaatggtt
     2401 accggaagaa catgatatta tccaactaat caaagaatta gaacaacaag tttctcagtc
     2461 ttatccatct agttatcact atgtccaacg aattctggaa ttaagtaaga tcaaattaga
     2521 tattgaagac acaagtatgg atcggatttt actgacgatc tatctaaaaa aagcgaaatg
     2581 gtcaaaagaa aaacgactgc caaaagcgat gattgctgct cacggttatg caactgctag
     2641 cagtatggca aatgtagtaa atcgtttatt acaagaggat ctatttgaat catttgatat
     2701 gccactagat gtcacaccac agcagattgc ttcagaaatc cttgattatt gcgaaaacag
     2761 cgatgtttca aatggattga tcatattagt agacatggga tctctgaaag aaatccatca
     2821 gttttttaaa aaacagttat ccgtaccgct tttgattttg aataatgtga cgacaccttt
     2881 agcaatcaca gtaggggaat gcctgcaaaa aaatgtatca ttagaagaga tagcagaaga
     2941 gactgttcga cagatccagc cagaatggcg actgctctat ccagaggaaa acaaacccaa
     3001 agcactcatt actacttgct tcactggaat cggtacggcg gtccacttaa gtgaactgct
     3061 agaaaaaagc ttacctacgg cttgtcagtt gaaaatcatc ccatatgaat atcagcaatt
     3121 aaaagataag aaaaacagtg acccattatt ttccatatac gaagtcgtag gaatgattgg
     3181 caccacagat cctgatatca ccaatatcga ctatctttca ttagaagacc tcatctcggg
     3241 ggaaaagatg tctgtactag ctgaatggtt ggacactact atgaatgctt cagagaagga
     3301 aatattcaat cagcgcatca ttcgtaattt ttcattggaa aaagtactgg attccgtgac
     3361 tattctagat acagagaagg tgatgggaga aatcgatcat tttatgagag aattagaggt
     3421 gcagttgaat cgaagcatca gcaatcccaa aaaattagcg ttgtatgtcc atgtgagctg
     3481 tctcattgaa cgattgatcc gacaaatgcc aatcgaaacc tatcaaggat tagagaaatg
     3541 gaagcagtgt caaaaagaag ggttatctgc tattaaatct gcgtttagtg tcattgaaga
     3601 gcattatagt gtcatgatcc ccaattctga gattgcctat atctatgata ttttgagtga
     3661 agatacagag tttctctcga tagaagaaga attttgaaca taagcaagtt gttgccacta
     3721 attttggcat gcaacttgct tttttagtat gtgtaagtga aaagaggaga tgaaaaagtg
     3781 gatagagaaa ttatcttagc ttcccatgga aaatttgctt cagggatatt agattcatta
     3841 gagctgatct atgggaaaaa tcattctatt actgtattcg attgctatac tgatgagaat
     3901 tttgatttag cggaaacagt aaggcagctt tttatgaggt acaaagataa agaaatcatt
     3961 gttctgacgg atctttttgg cggaagtgtc aacaacgaat tcttacaata tatcaatcgg
     4021 gatcatgtct atctcgttgc cggattgaat ttgccactat tgattgagct agtatctcgg
     4081 ttggacgctg aagtagagac aactgttttg atccagcagg tgctaactga ttcaaaaaaa
     4141 atgatccagt tttgtaacga gcggctcaat cagaaaatag aagaagacga attttaggag
     4201 gaagaaaaat gattgtattg acaagagtcg accatcggtt attacacgga caagtagctt
     4261 tttcatggac acaaacaata ggcgcagact gtattttgat tgctaatgat gatgtaccta
     4321 cgaatgagat acgaaagaca acgatcaaat tagccaagcc acaaggagtc aaactagtga
     4381 tcaaatcaat tgatgactcg attgccgcgt tgaaaagcgg ggtgacagat aaatataagt
     4441 tattcatcgt agtggaatcg atcgaggatg catataagct ggcagaagcg tatccagaaa
     4501 tcaaaaccat caacttagga gggatcaagg caaaacctgg tactcgcagc atcggaaaag
     4561 cggtaaatat tttaccagaa gaggaggaac gcttgaaacg attaattgac aaacaaatag
     4621 aagtagaaat ccgtcaagta ccagcagata aaaaagtgga tgtaagagaa gttttgtaag
     4681 aaaggaagaa cctcaatgat tgtacaagca gtcttattag gaattgttgc atttgtcgca
     4741 caatcagaat atgcactagg gacatcgttg ctttctcgtc cgattgtgac ggggctgttt
     4801 acaggaatcg tgttaggtga tgtaaaaact gggatcatta tgggcgcaac tttagagtta
     4861 gcatttatcg gatcgttctc agtaggtgct tcgattccac ctgatgttgt gaccggtggg
     4921 attttaggga cggcttttgc tattacagct ggagccggta cagaaaccgc gcttttacta
     4981 ggtttgccga ttgctacgtt gacattgata ttgaaaaata tttacttagg gcttttgatt
     5041 ccaattatga accataaagc agacaattat gcagaagaag gaaattataa aggagtcgag
     5101 catatgcact tgcttgccgg tatcggtctt tccttgatgc ttggtgttgt ggtaatggtt
     5161 tcttatatgg tgggaagcaa tacgatcgga aatctattaa atatgattcc tgacttcgtt
     5221 caaaaggggt tgtcagtagc tacagggttg attccagctc ttggttttgc gatgcttgct
     5281 cgtttgctga tcaacaaaca agttgcacca tattttttct taggttttgc tatagcttct
     5341 tacttagaga ttcctgtgac cggagtagct atttttggtg cgattttagc agttgtggta
     5401 gtcaatatta tgaatgtccg tcaactacca cttcaaacaa ctgcagggga ggtagaagaa
     5461 gatgaagaat tctgagatca aaaacagcaa aataacgaaa aaagagctaa atcacgtatt
     5521 ttggcgttct tttcaaatgg agttttcttg gaattatgaa cgtcagatga atatggctta
     5581 tgtttatgca atgattccga ttttgaaaaa gttatatcag tcgaaagaag aaatggcagc
     5641 agctttgaaa cggcatctag agttttttaa cacgacccca catatcgtta cactgatttt
     5701 aggaatcacg gctgcaatgg aggaagaaaa taaggatgat cctgaatttg atgtgactgc
     5761 gatcgatagt atcaaaacat ctttgatggg acctcttgca gggttaggag attctttctt
     5821 ttggggaact ttgcgattga ttgccacagg tgtgggtacc tctttagcca tgcaaggaaa
     5881 tattttaggc cctatcttgt tcattttgat tttcaatatt ccgcacattc tgtttcgcta
     5941 tctagcaaca ggttggggat atcgcttagg aacgggtttt ttgaaaaaga tccaggcaaa
     6001 cggtatgatg gaaagtttga cattaggcgc gtctattatt ggactgatgg tcgttggagc
     6061 aatgactgcc acgatgatca ccatcaatat tcctttaaaa atcggttcag gagaaaatgc
     6121 tgtgactgtt caaagtattt ttgatgatat cgttccaaat atcttatgtc ttggagcatt
     6181 tggcatagtg ttttatctat tgaagaaaga agtgaaacca ttaacgatct tgatcggact
     6241 agctttcttt ggaatttttg gttcgtggat agggattttt taaaaaagga gcagagaaaa
     6301 tgtttacgat gcaagattat atctatgaag aaaaggaagt actaagtacg atcttgaaaa
     6361 agaatgattt ttcgacaaga gaacatatga aaaaaacagt gaatttactg atcttagcaa
     6421 cgggttcatc ttataacgct tgtttagcag cgaagccagc attggaatct tatggtgatc
     6481 ttactgtcga tattcaagaa cctttttatt ttttcaatta tggaaaactt tctccttcaa
     6541 tcgatactgt gattgctgtg tcacaaagcg ggaaaagtgc ttctactgtt gaagcagtaa
     6601 aaatgataca aaaacaaggg ttacctgttg ttgctatcac aaatgacgtg caaagtccac
     6661 ttgctttaga agctgcacaa attattgatt taggtgccgg ggtagaatca gtaggcttcg
     6721 ttacaaaagg ctattctgcg actgttttac agttactact cttagggata ggaattggaa
     6781 taagtaaaaa taaaatcagc aaaaaaatag aacaagacta catgcaacag ttgcgcaaaa
     6841 taatcaacca tttaccagcg attattcaaa aaacggaaga attttttgat ggatatcaaa
     6901 gtttatttcg tttagcacag cgtttcgtca ccattggata tggtccaaac tggggaacag
     6961 ctaaagaagc agagacgaaa ttgactgaaa caatccgcgt tccgtcacaa ggatttgagt
     7021 tagaagctta tatgcacggc ccatacctgg aggcagatgc ttcgcatttg ctattcttta
     7081 ttgaagggga aagtgtaaat aaggaacgtt cgcaaaagtt gcaaagatat atgagtcggt
     7141 acgttggaga gactctcacc attaccacaa aaaaagcaag aaacgagaaa acacttggat
     7201 tagcaataga atgtgatgaa tatctgtctg tattagcgct tgtcgttccg tttcaactat
     7261 tcgcttataa aaccgcagta gctaaaggta ttgacttgaa caaaaagatt tttgaagact
     7321 ttgatacagt cttgaaaagt aaattataag aggtggaaat catgctgaaa ttcaatgaag
     7381 aagaacaaat caaagcaata aaaggcgcac tagctttacg tccgcaagta gaagaaatca
     7441 tcgataaact ttatgcggaa aaatttgacg ctgtctatta tctaggaatc ggtggaacgt
     7501 atgcttcctc gatgcaagct gtcacttata tgaatgggaa aagcaacttg cctgtctttg
     7561 tacagcatgc tgcagaatat tacactactg gaaacaaaag attaacaaaa gattctatcg
     7621 ttattttgtc ctctgttacc ggtacaactc aagaagtagt gaaagcagta gaacaaatca
     7681 agaaagtagg agcgacactg attggattta tcgataaagc gaacagtaag ttgtctcagc
     7741 tatgcgattt tgtggtcact tatccagctc ctggaacaga acaaatcaaa ttttttatgg
     7801 cagcagatcg attgatgtat ttgaatggag aattcgaagc ttattcagaa tactatgaac
     7861 agctcgaacg ttatttacca acagggttag tggaagcaga gaaaaaagca gatgcatttg
     7921 gcttatcatt tgcagaaaaa catcgacgtg atagtatgca ttattttatt ggagcaggga
     7981 atcaatgggg agctgtttat tcatatgcca tgtgttactg ggaagaacaa agctggcttc
     8041 catcgaaatc tatccatgct gccgagtttc ttcacgggac gttagagatc gtagaagaaa
     8101 caaccccagt cacattattt ttaggcgaag atgaacagag agtgttatct gaacgtgtgg
     8161 ctaaactttt gccaaaaatt tgcagcaatt atacattgat agacacaaaa gactatccag
     8221 tagaaggtat cagtgaaaaa tatcgcggac gtgttttgtc tttcttattg atgcatgtag
     8281 tgacacaacg catcgatgct catgtggagc agttgaactg ccatccatta gagatacgcc
     8341 gctactatcg tcagtttgat tattaggata gactggataa aaagaatata aaaaataaag
     8401 agagcagcga taactgctcc ctttattttt ttaaaatatt ggcatcaatt acgttataaa
     8461 acacattttt tgcattatct acagttcaac ttataatctg tttaatggga catgtgggtg
     8521 ccaaccattt tttgttatgc aatagtacta tttagttgtt gcatggatcc gtagtaaaag
     8581 tccaagtaca acgagttgcg ccagtttaca tagcgttttg tatccagaat gttgactaaa
     8641 acaaaagttc ctatgtcaac cgtcaacaga gacaatctgt ccgttgcagg acctgacaga
     8701 tttagaaatg ttcaatctgt ctcttatgta ttttaaatca atactcacta ggagaagttt
     8761 cctaacctcc taatagtaat gctggtagta acattgtcaa aactgcttcc tcttaggaac
     8821 aagcaaagca taacgagtgt aaaaataaaa tgtagactat tgtaacatat cattgattat
     8881 ttcaagtaaa aacgataaaa gaagtaaaca aacacttaaa aacaatggaa attagtccca
     8941 actttaagaa atgtatacta taatggaaat tatcaattat ttatttggag gaatatccat
     9001 gaaacaaaaa atcgcagttc ttggtcctgg atcttggggc actgctttag cacaaacatt
     9061 ggcagaaaac gggcatgatg tccgtatttg gggaaatgta cctgaacaga ttgatgaaat
     9121 caataagtat catacaaatc agcatttttt acctgatttg acgatcccag aatcgattat
     9181 cggatataaa gaattggcag aagcagtgga taacgcagat gctattttat ttgttgttcc
     9241 aacaaaagcg attcgttcag tcgcacagga acttattggt aaaatgaata ctaagccaat
     9301 cattatacat gcaagtaaag gactagaaca agatacgcat aaacgaatct cagaagtttt
     9361 ggctgaagaa attccagaag aaaaaagaca ggcaattgtt gtattatctg gtcctagcca
     9421 tgctgaggaa gtggcagtac atgatatcac aacaattaca gctgcaagta ttgatcaaaa
     9481 agcagcagcc tatgtacaaa aactgttcat gaatgattat ttcaggattt atacaaataa
     9541 tgatgtgatt ggcgtagaaa ctggtgcagc actgaaaaat atcattgcaa ttggtgccgg
     9601 cgctatccat ggattaggat tcggagatga tgcaaaagca gcaatcatga ctcgaggatt
     9661 ggcagaaatc agccgtcttg gtgtcgcgat gggtgccaat cctctgacat ttatcggttt
     9721 gagtggtgtg ggggacttga tcgttacttg tacaagtgtt cattctcgca attggcgtgc
     9781 cggtaacttg ttaggacagg gacataagct ggaagaagtt ctagaaaaca tgggaatggt
     9841 cgttgagggt gtggcaacga caaaagcagc agtagagcta gctcaacagc taggtgttga
     9901 gatgccaatt actcaaacaa tctataatgt tttgtataat ggagaagata tcaaaaaagc
     9961 agcaaaagaa atcatgttac gtgacggaaa aatggagaat gaattttctc ttcgttaaat
    10021 agatgatcaa tagttaatcg tc
//
LOCUS       DAXWOO010000102        10024 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.103, whole genome shotgun sequence.
ACCESSION   DAXWOO010000102 DAXWOO010000000
VERSION     DAXWOO010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.103"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(29..226)
                     /locus_tag="ACGE70_001963"
     CDS             complement(29..226)
                     /locus_tag="ACGE70_001963"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFF8693590.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            complement(372..1250)
                     /locus_tag="ACGE70_001964"
     CDS             complement(372..1250)
                     /locus_tag="ACGE70_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFF8693591.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(1289..2407)
                     /locus_tag="ACGE70_001965"
     CDS             complement(1289..2407)
                     /locus_tag="ACGE70_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFF8693592.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(2404..4503)
                     /locus_tag="ACGE70_001966"
     CDS             complement(2404..4503)
                     /locus_tag="ACGE70_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFF8693593.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(4554..5375)
                     /locus_tag="ACGE70_001967"
     CDS             complement(4554..5375)
                     /locus_tag="ACGE70_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFF8693594.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(5372..6181)
                     /locus_tag="ACGE70_001968"
     CDS             complement(5372..6181)
                     /locus_tag="ACGE70_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFF8693595.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(6202..6696)
                     /locus_tag="ACGE70_001969"
     CDS             complement(6202..6696)
                     /locus_tag="ACGE70_001969"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFF8693596.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(6708..7208)
                     /locus_tag="ACGE70_001970"
     CDS             complement(6708..7208)
                     /locus_tag="ACGE70_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFF8693597.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            complement(7273..9969)
                     /locus_tag="ACGE70_001971"
     CDS             complement(7273..9969)
                     /locus_tag="ACGE70_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFF8693598.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
BASE COUNT         3047 a         2261 c         1654 g         3062 t
ORIGIN      
        1 atttatttta tacggctgaa aaaatctatc accaaaaaaa ttcaaacatt aagccaagga
       61 ttatgaagtc aacatttcga aaaatgaaca tacattggta agcaactata actattacca
      121 cagacataaa gtaacacaat atattactat ttaagtgtac gccaccagtg aatatcctca
      181 aggtatacag cgcacaacaa aaagaaaaaa ggaaacgcag aagcatttcc tttcagtgat
      241 ttaatgaaaa caacacgatt tgacttagag cctttttatt tgaattcatt ttcgctttat
      301 ccaacttcat ttgatcattt gattactcgg ttaaatactt ttgacatgag tatcaatgaa
      361 acattcatta attatggttg cttatttctt accgcttttc tggccaatgc taatccgccg
      421 attattccgg cattatcacc taattcgggc gtaataatgt agctatcaat tggtggtatt
      481 tcaacatatc catttataag ctcaacaaat ttatcctgaa cttttttctt catgtgttct
      541 tgtttcatta caccaccgcc aaaaataatt ctatctggcg aaaacatcag tgttgtattg
      601 tatgcacatt gggcaaggta aaaggcttca atctgccaaa acgaatcgtc agccaataaa
      661 ttctggcctt ttacacccaa ccgtttttca atcgctggtc ctgctgccat cccctctaaa
      721 caatcttggt ggaagggaca gtttcctgaa aaattgtcct ttggatgtcg cttgatcaac
      781 atatgcccca tttccggatg actgaaccct tccacgaaag tcccattgaa caaggcacca
      841 cctccgatac ctgttcccac agtgaagtat gcaacacctg ctaacccttt gccagaacca
      901 gcaacatatt caccatagca agccgcattg acatctgttg tccattcaat cggaatattg
      961 aaatattgct ttagcgttcc tacaaaattg aaattttgcc aagcaagttt tggtgttgat
     1021 gtgatatgac cgaaactcgt tgatttgcat tgaatatcaa ttggaccaaa gcagccgacc
     1081 ccgatgcctg ccaattgttc cttaaaaggt ttaaaaaaat caatgacctg cttcattgtt
     1141 tcaactggag ttgttgtggg aaaactgact ctttccaaaa tcgttaattc ctcagtaccg
     1201 actgcacaga caaatttagt ccctccagct tcaattgccc catatatcat cataggttcc
     1261 tcccttcgaa tatgtgaaaa acacttcatt aacgcggcaa gcgttctcga tcagtgaaat
     1321 gacgtttagt atttcctgaa gcttctgttg aataccagta agcgacacta gcaacatcgt
     1381 cttgccgctc aaataagcga acatcatcgt tgccgatttg ttgtaaagtg accgataaat
     1441 cttcggtaaa acagatgggg tcttgaagat gccaacgata aagaccatgt cgtggcattg
     1501 cgtcgtcacc aaaggcatga acggcattca attttcccgt ttcaaaacga tctcttgtgg
     1561 catctctatt tgattggtaa ggatacccta aaaaggctgt tgaatagttt ttcgcatgtg
     1621 gacgtccgaa ctcatcgtac tcatgaaagg cccacgcccc gccaaaataa tcttctgaac
     1681 ctgttgaggt tacggtcgga tactcactat ccccatcaat gtagaattta aactcgcctt
     1741 cgccccacca ataacgttct aggcaggtaa gcgcaaggta agtacccacg taatagcctt
     1801 gaccctttaa tcgatcaatt aacttataat cctcctgtaa tttggtaatg cgttcccgat
     1861 gccaatttgc gtgaaaatat aggctatttt cttccaattc gtccaccaaa gcgtaattta
     1921 tcgtataaaa aaatgaggac atatccttcg gatgtaaatt ctcgatcgta attttagcgg
     1981 atgtttggaa aggcatttcg aaataggaat taaagccccc tgtaggatta accactatcg
     2041 gcaacgagtt cacctcgcaa cgctctccaa acccattaca aaagaaatcc cctaaaggac
     2101 tttcaacagc agggctatcc gacccatccc aataaattct taagattaaa tcacgtaaaa
     2161 caaaactgcc aaattctgtt cgatcacgaa tcgtcatcca catatgacga attattcccg
     2221 ttccagtaat atccgcaatc acagtttctt caccagaagt cagcggaatt gcgccacgac
     2281 ctttgcggct tggtcctagc tcgcttggca acatcgcagc tttacctttc tcgcctgttg
     2341 gattttcggg agtgattgct cgactctgga tttttttaaa ttgggtaata ttttttagta
     2401 tactcatctt tttctccttt gaatgtgaat cgtttttccc ttttctacgt gaagaatttt
     2461 tttcgtatca cttattttca atggcttttc tgacaaattt gaaatactca actcggattg
     2521 cgtacattca atcgaaatga aggcttgacc gaacttcaaa ttcttccaag aaaacgttaa
     2581 attatcaaca ggatcaattt gcaatactcc ttttttgaat ttcaatccga aatattgact
     2641 gatcattagg ctgacaaaaa gacccgatgc ccagccgcat ttgccgcagc caaagtctcg
     2701 cacgacttct ccttgttgag cctctaagcg atacggccac caccaccacg aaccgtctaa
     2761 atctgccaaa caaattagtt cttctaatcg ggttacgaaa gcttgagggt tgtgcagctc
     2821 gctcattaaa acagtgataa agccaggaaa agtggcgccg gattctaatc cccaagtgat
     2881 gcctttgatt tcctcgctat aggttggatt gaaagaacta gcagaaaagg ccatggtgtt
     2941 ctgatataaa tgatctttat tatctaaaaa ttgataaaaa ggcatcaacg tagtgtctga
     3001 ttcttctcca tcatgcatca ataaattaat ttttccatcc acaatcacat cacttaaaaa
     3061 gattaaccct tgctcaagaa tcggtttttg atagtgcttc actgaatagg ttgttttctc
     3121 ctcatcaccg attccctcca aaaattgctc accaaaagga ccatcgatag tcatttgttt
     3181 acaaattgat tcctttagtt tacaggcaat gtttttgtat cgcttgctaa gactcagttg
     3241 ttctagggct tcaaaaattt ctgcaagtcc aatacaggcc ttccaaagac aaagattgct
     3301 gccggtatga tactttccaa gtgcaaaggc gtctgataac caaacagatg gaaaaagcat
     3361 tggttcccct gaccgacgtt gagctaatat gtcctcaata attcttgaag cattcagtag
     3421 aacctgcgga caccgctcaa agaattttgt atccccagta tgttcaaaat aaagactagc
     3481 cagtaacgca ctggataacg aattactcag agaatgattg atacctcctg taaattttga
     3541 accaggaaac ttaacgccat attgatcaaa ccatagaacc gttttttgac ataactccgg
     3601 ttcaaacatg gtgaatggca atgatgcgta atacatatcc ttattccaaa ttcgattagt
     3661 agcaggatag ctgccccaat tgctgccgac gacctgtccc tgtcgattca taccgaaact
     3721 agaaaagctt tgataaagtg cacgacgata gagatggaca actctatcgt ttggcattga
     3781 cagctgccca aatagctgag caaaatagtt caatgtttcc ttcagccatt tttcgatatc
     3841 agattcctga aaagtcatta cttcttcaaa agcatttggg tccacaaaag caatggcgaa
     3901 gcttgccact tcctgttgtg cgatcgtgtc cttatttcct ttttgagaaa tcaacacatt
     3961 ttgctgatcc gaatacttct cttgatacga aggcacatct ttcactgaaa cttgaaggct
     4021 ttcgtgtgtt tcattcacaa tgtaaacctg ttgaacaagc atacttaaac gcttattagc
     4081 cagtatggga gcaaaggata ctacaatgac cttaaacgtc gaataatgat gaatatatct
     4141 aggcaaacag tcttgaatca catcgacttc aatacgatga tccgattctg acaaatggta
     4201 gacaatcccg tttacttcaa cctttaagga taacgaattg gtttgagaga gctgtttatt
     4261 gacccaaaca cctggttttt cctcagttag taaattttct cgataaaagg tcaacgtttg
     4321 aatcgtcccg caaacatcaa ggtttgctaa tgtcttttga ttacttacat cgaaggtcaa
     4381 tccctttaca tcaggaatat tccgatagta gctgtattcg tttgaagtag tcacatttcc
     4441 gtcgtttaac tcagcattaa aaaatgggtg tttgggtgaa taaattactt cattaatagt
     4501 catcttttcc ctccttgcta aaaaaactgc agcctagtag ctgcagcttc ctactatgcg
     4561 attccaagta atgacaataa aattcccaag acgaccattc caaaaataat tttgatgacg
     4621 ccgacttttt tatctagtaa tttgaaacag ctcaatgtta ccaataacgg tacaattcct
     4681 ttaaaaatac tatcgaggat ttcttggaca ttcattagtt cttcgccctt cattgaaaag
     4741 ctcaaaacgg tattgaattt gaccatactg atcgtcattg cacctaccat gatcagaccc
     4801 aagatactag cagcttttgt cagaatttca attaacccgt tcgcatacag cttttcaata
     4861 tatttagagc ctaatgaata gcctaaaacg ccgccatagt atcgcatcaa gtaattaggt
     4921 atattgaaca taactaaaaa gataattggt gccaaaatat ttccggcatt ggctaagttg
     4981 atcgctagtc ccgcggtaac gacacgccaa actccccaga acaatgaatc gccaattcca
     5041 gccaatggtc ccatcaaact cgttttgatc gcgttgatac tttccacgtc aaaaccagct
     5101 ttttcagaat tttccttctc catcgatgcc gcaatcccca tgacaaagga cgcgcaagga
     5161 atggtcgtcg aaaaatactg tgtatgacgt tccagcgccg cagctcgatc ttcttcactt
     5221 ttgtagaaat gattaatgaa gggcatcata gaataggcga aaccaccgcc gcccatcgtc
     5281 accggattca ccgaagcata catagtcatc gagcgccaaa acatcttccg aaccaatttt
     5341 ttctcatctt tagaaattcc atcagctaat ttcattgaaa gaaatcctcc tcttcttggt
     5401 caagcgttgc atttacagca gcatcgcttt gaactgcatg aacagatata ttttttagtt
     5461 gtagatctct ttgggctata acaattgcaa taatcgttcc aactgcggca attgcaatca
     5521 atggtaactc gagatacgcc ataacaacgg ccccaaagaa aaaccacaca gcaatatttt
     5581 tactccaaag catccgtaac agcatcgcca tcccaactgc cggcagcaga tttccagcga
     5641 ctgttaatcc gttcatgatg acctctggaa tattatccaa gacgcttttg acaacatctg
     5701 ctccaaataa aacggcaaag aatggaataa gagcataaag cgcatagtgg acaaaccaca
     5761 taaagaagtg cagattgatg attccttttt gatcaccaga ttttgcaaat ttgaaaaatt
     5821 tagcagccat cggtccacat acaaaagaaa aaatcatatt cattaactgt aatcctaata
     5881 ctgctagtgg aaagacaatg ggaataatcg catccacgtt tcctcccatt ttgattgcga
     5941 acgctgtagc cagcccagca gccaaagaag gctccgccgt actagagatt ccgatattga
     6001 cgacacccat aaagatggct tctagtgacg ccccgacaat caaccccgtc tgcaaatccc
     6061 ccatcaacgc tcctgctaac ggagcaacaa caatcggacg actcagcatg gacatcccga
     6121 acatttcatg tccagcaact ccaataaaaa cggccaaagc gactacaaat gcatcataca
     6181 taatttttct ccttcctttt tctaaaaatc actcaaaata gagatagctg aacgtttttc
     6241 catcgccggt gtaggctgaa aaacggtatc tggataaagt tcaactaatt cttttaagtc
     6301 ttgaatttct gacgtcgtta acatcacttc ctttgataag gttttcttat cttccgatgg
     6361 cggtgtcatt ttcccagcat tccccacgtt cattaattcg atttccgttg ggaaagcttt
     6421 cgcaactttt accgcatctt tgacattagg gactaaaacc atcaagcgca tgtccttcgc
     6481 ccgcggatct tttaacaaat gaatcgcgtc atcaactgtt ttaataatga ctttgacatt
     6541 cgatggtgca gccatcttca atgtcatctt ttgaatatcg ttggtagccg cctcatcgct
     6601 tgctactact aacccggtta aatttagact ctttgtccaa gtcatcgcta cttgtccatg
     6661 aattaatcgt tcatcaattc gcactgcttt aatcattttt cttcctccta aaaaaaatct
     6721 tcctcttcat ttactacatg tacctcatca agaatggtca tctttaattc ctgttggcat
     6781 ttatttacca gttcctgaat atcctccttt gttaattcct tggttgccaa tactatttcg
     6841 agcagaattg gcagattaaa gccagacaaa atagttacag caatctcttt ctccaaaaag
     6901 gttttggtaa tcttctgatt gacacttccg ccaaataaat cggtaaaaac aaaaatctga
     6961 tcctgctcgt tcacctggtt tacaaataca ttcaactcct gttcaaaatc acactcatcc
     7021 acataagcat taatcaccgt aatctcttga accttccccg tcagtaattg aatactgcta
     7081 agaaagcctg aagctaagtg accgtgagtc gctattagaa ttttcttcat atggttaatt
     7141 cctttctttc tgttcacttt tatataagca attcctgtgc caacttttaa aaacattggt
     7201 ttaatcacgt taattcacat taaaaaaagt gtctaaccta aaaaaaacag attagacact
     7261 tttttagaca ctttattttt gaatatacgg tcctaataat tcatagagca gcgaaagctc
     7321 ataagcattg actttaaaat tatatttcat ctccattgat tgaaaaatac ttctagaaac
     7381 ataataaaat tcatcttcat ctggtttcaa atcataattc ggttcttcag atttttctgt
     7441 ccgagccaag aataaacgtt ccatcatcaa tgccgtatgc atatagagat ttagttttac
     7501 acggccattt aaagtgaaat gatagtaatt ttcatattta gaaatgactg tttccacttc
     7561 actcatcacg atcgttgggt tcagaaagtt caaacggtcc gcaattcctt ccaacgaaaa
     7621 gaattttact agctcctgaa tcaagcggtt aaattctgcc ggcttcattt caggactcaa
     7681 aatatgctcc aaattggctg ctccatcacc gtctaagatg tcataaacat taatattagg
     7741 aattgaaaag gaacgtggaa gatccgaagt cgtgatgacc aattttgtct tgtcaaaata
     7801 agtttcatca ttttggtcga ttaattcacg tagttctctg tagtccttcg tgaaaacttc
     7861 gatggattca tccgataaat gatgacgaaa aacatctttc aatttgtccg aaatccccaa
     7921 acccgacata caggaaatga taatgttgct actttgagaa aagccttcaa agtattgaac
     7981 attgattgaa tacttgtctg ctgcatcctc cgcaatctgt ttaaatggcg cattcatcgt
     8041 catcttcaag ccaatatcaa gtgcaatgga agtcgtcaaa ttattcagaa gaagtaactc
     8101 cccttttaaa tcgttcttga tctgtgtgta aatttgattt aaagagccca tatctacgat
     8161 cagcactaga ctatctgtat tcacttgccg tttcacgaaa ttccttgtcg attcgacaat
     8221 ctctgacaca ttactctcta ctggcatatc caacgcttca aaaacataat tgccgcataa
     8281 ttgattcact actgcttgaa tgctgctagc cgtactatct ccgtgagcaa gcaacaggcc
     8341 cttcagctcg atggattcat ctacataatc agataaagtc acgacaataa gcagtcttag
     8401 aaagttctcg tccttttctg agagcggtgg taaatgctcc aagaactgat tacataagta
     8461 agtcgtccga ggtagatttg cagctaatgt acgatttatc ccggttaagt ctgtcttata
     8521 gcttggggaa atgacatagg atctgctaat aatttctgca acaggtccac tcgtcgtcaa
     8581 tccgtacgtt tttcctaaaa cggataccca gacctttcta aacatctgaa tcatcaattt
     8641 tctactcatt gataaaaaag aatccttatc aatgtgatgc accaaatgct gtacttcatt
     8701 ctttatcgaa gaaaaatttt cattgaaatt accttcattt atcagctgag taattttttc
     8761 caaaatcgaa tcataatcaa acggtttgat tcgatccgtt tttatctttt gatttttctt
     8821 tatcaataaa ctagcaatcg gcaagctttc tacttcggat tccagcatat ttaactgaat
     8881 caagatctca tttttcccaa tcgttaatgg ttcttcttgt gttcgactat acacatccgc
     8941 acaactgatt ttaatgatat tcttcagctt accaatattc ccactaaagg gatgagataa
     9001 cattaaagac aaggcttctc ttgaaacaat aatatctttt tgtaaaacat ccgcttcatt
     9061 ttgaaaaaat aattgaatca gctctaatcg ttctcgaatg gggcgatctt caaaagttgg
     9121 caacataaca ctaatttgaa tacgccgatc aaaggtgtct aataaataat tttcaccttt
     9181 ttccgtcgtg gcgaaaataa aacgaacctt agcagaatgc caatttacat tttcaccgac
     9241 tggacgatag tttccagtat caataaatag aaagagcttt tcttgatttt ctttcgatag
     9301 gcgatggacc tcatcgagaa aaagatagcc gttgttggcc gttgccaata aaccatcggt
     9361 atccttttca gcccccgtaa aggccccctt cttgtatcca aacagtgttg ccgaaagcag
     9421 ttcgggattg tccgcataat cggcacagtt taagatagca aatggtgcat ctttttcaat
     9481 aatttgctca tggatagcgt attcaaaaat ttttgtggct aaaaaacttt ttccaacacc
     9541 cgtggctcca tttattaata cactgagtcc gttaggcgga tatttgacag cggctataca
     9601 tttttgaatg acctctctca gacttccgtc atgaccaact acagaatgaa aggcttcgtt
     9661 tatttgctcc gtattatcta caaccgcata ttcatctaaa ggcttccaca aaacaggctt
     9721 tcccggtaat ttctcgacct ttttatcttc cactaatctc gtcaaataat ggctggcatt
     9781 ctgacgagag atacctagat tcttcgaaat tttctcggca gtgacagaag acatattccc
     9841 tgttagtcgt atcagctcat caaaaactcg atctttagcg ctctttttca ttttttcctc
     9901 cagtttaatg tgtctaaaag cataataaac tatcggctgc aatatgtcta ttctatattt
     9961 gtattccaat ttttactttg aaacacaaat agtcgaatat ttgcctcaga tatctgacaa
    10021 aata
//
LOCUS       DAXWOO010000103        10022 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.104, whole genome shotgun sequence.
ACCESSION   DAXWOO010000103 DAXWOO010000000
VERSION     DAXWOO010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.104"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(<1..722)
                     /locus_tag="ACGE70_001972"
     CDS             complement(<1..722)
                     /locus_tag="ACGE70_001972"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFF8693599.1"
                     /translation="MTYKNIVFDLDDTLYDHQLPFKRSVEKCFPTIDIQQIDNIYKRF
                     RCWSDVAFPKYTKKLISIEQLRIFRCQKTMEEFGIDSISRTEALDFQADYEYELDQIT
                     MIPEIHQLLLALHKNRIPIGILTNGPVDLQSRKLQNIGAYQYFEKQNIIISQSTHGGA
                     AENSYEGNVKNLQSYQNVQLSKRAIEILKEEIELNHQHIRFNPDYKDNGWIFTSKSRH
                     KPGMATVFLTNFIRCRTSFRML"
     gene            complement(727..1494)
                     /locus_tag="ACGE70_001973"
     CDS             complement(727..1494)
                     /locus_tag="ACGE70_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFF8693600.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
     gene            1983..2408
                     /gene="lacA"
                     /locus_tag="ACGE70_001974"
     CDS             1983..2408
                     /gene="lacA"
                     /locus_tag="ACGE70_001974"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050044 - galactose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HFF8693601.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            2425..2940
                     /gene="lacB"
                     /locus_tag="ACGE70_001975"
     CDS             2425..2940
                     /gene="lacB"
                     /locus_tag="ACGE70_001975"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_function: GO:0050044 - galactose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HFF8693602.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            2951..3883
                     /gene="lacC"
                     /locus_tag="ACGE70_001976"
     CDS             2951..3883
                     /gene="lacC"
                     /locus_tag="ACGE70_001976"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009024 - tagatose-6-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HFF8693603.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTISHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            3886..4866
                     /gene="lacD"
                     /locus_tag="ACGE70_001977"
     CDS             3886..4866
                     /gene="lacD"
                     /locus_tag="ACGE70_001977"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFF8693604.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            4894..5211
                     /locus_tag="ACGE70_001978"
     CDS             4894..5211
                     /locus_tag="ACGE70_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFF8693605.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            5211..6917
                     /locus_tag="ACGE70_001979"
     CDS             5211..6917
                     /locus_tag="ACGE70_001979"
                     /EC_number="2.7.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0019197 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase complex [Evidence IEA];
                     GO_function: GO:0015155 - lactose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactose-specific PTS transporter subunit EIIC"
                     /protein_id="HFF8693606.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTLLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            7059..8465
                     /gene="lacG"
                     /locus_tag="ACGE70_001980"
     CDS             7059..8465
                     /gene="lacG"
                     /locus_tag="ACGE70_001980"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFF8693607.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRLKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            8488..8761
                     /locus_tag="ACGE70_001981"
                     /pseudo
     CDS             8488..8761
                     /locus_tag="ACGE70_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
     gene            8782..9681
                     /locus_tag="ACGE70_001982"
     CDS             8782..9681
                     /locus_tag="ACGE70_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFF8693608.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDEDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSSLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
BASE COUNT         3164 a         1691 c         2006 g         3161 t
ORIGIN      
        1 aatagcatac ggaaagaagt tctgcacctt ataaaatttg tcaaaaaaac tgttgccatt
       61 ccaggtttat gacgactttt ggaagtaaaa atccatccat tatctttata atcaggattg
      121 aatcttatat gttggtgatt taactcaatt tcctctttaa gaatttctat agccctttta
      181 gataattgga cattttggta actttgtaag ttttttacat taccttcata gctattttct
      241 gcagctcctc cgtgagtgga ctgactaatg atgatatttt gcttttcaaa atattgatag
      301 gcaccaatat tttggagttt tcgactctgt aagtcaacag ggccattagt tagaatacct
      361 attgggatgc ggtttttatg tagagccagc agtagttgat gtatctcagg aatcatcgta
      421 atctgatcga gttcatattc atagtcagct tggaagtcta gagcttctgt cctagaaata
      481 ctatcgatgc caaattcttc catagtcttc tgacagcgaa aaattctaag ctgctcaata
      541 ctgatgagtt ttttcgtgta tttaggaaaa gcaacatcag accaacatct gaaacgtttg
      601 taaatgttat ctatttgttg gatatcaatc gttggaaaac atttctcaac gctacgttta
      661 aaggggagtt gatggtcata aagagtgtca tcaaggtcaa aaacaatatt tttataagtc
      721 ataattctag tccgctttta aaatagtagt gtactggcta aattcctcta agtgttgagg
      781 gctaatctgg ttatctgtaa tgatggtatc gagttgatct agattataga agttaaagaa
      841 atcgtatcga tcgaatttag tactgtctac taataagaat ttttctacag cattgtttag
      901 ggcaagttgt tgaatcacac cttccttgtc actatatgta gcaatagaat tatgggtaac
      961 agcatttgca ctaacaaaag ctttggcaaa tctaattgct tttaaatttg tcgaagccat
     1021 tgaacctaca aaagctccag ttatttctct atattcaccg ccaagaagca ataaatcaat
     1081 ggtttcgcta tcatttagaa tcaagaacac agggagacta tttgtaatga cacgaatttt
     1141 ataaccttta cgacctttca attctaatgc cagttgttca agtgtagttc cgggtccaat
     1201 aaaaatagta tcgccatctt tgattaaaga gcaggctttt ttagcaatgt caattttttc
     1261 ttttgtattt agacttttct tctcgatatg tgtcttttca agtggctttt tagaggaaag
     1321 taattgtgca ccaccatgag tacgtgttaa aagtccatca gcttctagag catcaaggtc
     1381 tctacgggct gtcatatcgg aaatatctag ttcatctatt atttctttta tggttatggt
     1441 cccatcaatc tttaacatat ctaaaatttt ttctaatcgt cgttttttgt tcatatgaag
     1501 acccttcttt cataaagtaa tttttttcca aagataattc tcttttaatt gtatcataaa
     1561 agacaatatt ttcaaggtaa aacaaacaat ttcaaacaaa aacaaatgtt agatgatgaa
     1621 ataagaacag tggattgacg tatattagct taggtcagat tttgtataag acgaaaataa
     1681 agtaggacct cttaatcagt aagttataga aagtaaaaga cttttgtaat acttgaatag
     1741 atatttcacg tccattttgt gatggattaa atgaacaaaa atgaacaata atttaacggt
     1801 gttatctatt ttttaagaaa acaaataaaa aaaacaaaaa ataaacaaaa aagtgttgct
     1861 ttttgtttga atgattgata ttatataaac aaagaaatga tgaaaacgtt ttttcgaaca
     1921 ttttgcaaaa tgttttctac ttctacgtag catttctttc taaaatttag gaggtggtca
     1981 aaatggctat tgttgttggt gcagatctca aaggtacgag attaaaagat gttgtgaaaa
     2041 attttcttgt agaagaaggt tttgaggtaa ttgatgtgac taaggacgga caagattttg
     2101 ttgatgttac cttggcagtt gcctctgaag taaataaaga tgagcaaaac ttaggtattg
     2161 taattgatgc ttatggagct ggtccattca tggtagcaac taaaattaaa ggtatggttg
     2221 cagcagaagt ttcagatgaa cgctcagctt atatgacacg tggacataac aattcacgaa
     2281 tgattacggt tggcgctgaa atcgtcggtg atgagcttgc taaaaacatc gctaaaggtt
     2341 tcgtcaatgg taaatatgat ggcggacgtc atcaaatccg agtagatatg cttaacaaaa
     2401 tgtgttaaga aaaggagtta aggaatgaga atcgcaattg gatgtgacca cattgtcaca
     2461 gatgaaaaaa tggcagtatc agaattcttg aaatcaaaag gacatgaggt ccttgatttc
     2521 gggacatatg accacgcacg tactcactac cctatctatg gtaaaaaagt cggtgaagca
     2581 gtagtaagtg gtcaagcaga cttgggagta tgtatctgtg gtacaggtgt cggcattaac
     2641 aatgcagtca ataaagttcc aggtgtccgc tcagcgttgg ttcgtgatat gacatcagct
     2701 atatatgcaa aagaagagtt aaatgcgaat gttatcggtt tcggtggaaa gattactggt
     2761 ggtcttctta tgaatgacat cattgaagct ttcattgaag ctgagtacaa accaacagaa
     2821 gaaaataaaa aattgattgc aaaaatcgaa cacgttgaaa cacataatgc acatcaagca
     2881 gatgaagatt tctttacaga attccttgaa agatgggacc gtggtgacta ccacgattaa
     2941 ggtataacaa atgattctga cagtcacact aaatccttca gtagacatct cttatcctct
     3001 agaaacattg aaaattgata cagtgaatag ggtgaaagat gttagtaaga cagctggtgg
     3061 aaaaggctta aatgttacta gagtcttgta tgaatctggt gataaagtta ctgctacagg
     3121 tttcttaggt ggtaaaattg gtgaatttat tgaaagcgaa ttagaacaat ctcctgttag
     3181 tccagctttt tataaaatct caggaaacac tagaaactgt attgctatct tacacgaggg
     3241 taaccaaaca gaaattttgg aacaaggacc aacaatttct catgaagaag cagaaggctt
     3301 tcttgaccac tatagcaatc ttattaaaca atcagaagtt gtgacaattt caggaagttt
     3361 accttcagga cttcccaacg attattatga aaaacttatc caacttgctt cggatgaagg
     3421 agtagcagtc gttttggatt gttcaggtgc acctcttgaa acagttctaa aatcgtcggc
     3481 taaacctact gccatcaaac ctaataatga ggaactttca cagttgcttg gaaaagaagt
     3541 aacaaaagat attgaagaac tcaaagacgt tcttaaagag tcacttttta gtggtatcga
     3601 atggatagtg gtctcattag gtcgaaacgg tgcctttgca aaacatggtg atgttttcta
     3661 taaggtagat attcctgata tcccagttgt taatccagtc ggatcagggg actcaacggt
     3721 agctggtatt gcatcagctt taaatagtaa aaaaagtgac gctgacttat taaaacatgc
     3781 gatgacattg ggtatgttaa atgctcaaga aacaatgaca gggcatgtga atatgactaa
     3841 ttacgaaaca ctaaactcac aaattggagt aaaagaggta taaaaatggt acttacagaa
     3901 cagaaacgca aatctttgga aaaaatttca gataaaaacg gtgttatctc agctttggca
     3961 tttgaccaac gtggtgcttt gaaacgtttg atggcacagt atcaagatac agaaccaact
     4021 gtagctcaaa tggaagaact aaaggttttg gttgctgatg aacttacaaa atacgcttct
     4081 tcaatgttgc ttgaccctga atatggactt ccagcaacaa aagcattgga taaagaagca
     4141 ggtcttcttc ttgcctatga aaaaacaggt tatgatacat caagtacaaa acgtttgcct
     4201 gactgcttgg atgtttggtc tgcaaaacgt atcaaagagc aaggcgctga tgccgttaag
     4261 ttcttgcttt actatgatgt agacagctca gatgaactta accaacaaaa acaagcttat
     4321 attgaacgtg ttggttctga atgtgtcgct gaagatattc ctttcttctt ggaaatcctt
     4381 gcttatgatg aagaaatctc agatgcaggt tctgttgaat atgcaaaagt gaaacctcgc
     4441 aaagtcattg aagcgatgaa agttttctct gatccacgct ttaacattga tgttcttaaa
     4501 gtagaagtac cagttaacgt taaatatgtc gaaggttttg ctgatggtga agtggtttac
     4561 agtaaagctg aagctgctga cttctttaaa gcacaagaag aagcgactaa ccttccatac
     4621 atctacctaa gtgcaggtgt atctgctaaa ttgttccaag aaacattgca atttgctcac
     4681 gactcaggtg ccaagtttaa tggtgtgctt tgtggacgtg ctacttgggc aggatctgta
     4741 gaaccttaca tcaaagaagg tgaaaaagca gcacgtgaat ggttgcgtac tactggattc
     4801 gaaaatattg atgaacttaa caaagttctt gttaaaacag ctagtccatg gactgataaa
     4861 gtatagtaaa aacataaaac ggaggatatt gttatgaaca gagaagagat gactctctta
     4921 gggtttgaaa ttgttgctta tgctggagat gctcgctcta aacttttaga agcgcttaaa
     4981 gcggctgaaa atggtgattt cgctaaggca gatagtcttg tagtagaagc aggaagctgt
     5041 attgcagagg ctcacaattc tcagacagct atgttggctc gagaagcttc tggggaggaa
     5101 cttccataca gcgttaccat gatgcatggt caggaccact taatgactac tgtcttatta
     5161 aaagatgtga ttcatcacct catcgaactt tataaaagag gagcaaaata atgcataaac
     5221 tcattgaact tattgagaaa gggaaaccgt tctttgagaa aatctctcga aacatctatc
     5281 ttcgtgctat tcgtgatgga tttatcgctg gtatgccagt catccttttc tcatctatct
     5341 ttatccttat tgcctatgtc ccaaatgctt ggggattcca ctggtcaaaa gacattgaga
     5401 ccttcttgat gactccttat agctattcga tgggtatcct agcattcttt gttggtggta
     5461 ctaccgctaa agctttgacg gactcaatga accgtgacct acctgcgacc aatcagatta
     5521 acttcttatc tacgatgcta gcgtctatgg tagggttcct tttgatggca gctgagcctg
     5581 caaaagaagg aggcttcttg acagccttca cgggaacaaa aggtcttttg acagcattta
     5641 ttgcagcctt tgtgacagtt aatgtttaca aggtctgtgt gaaaaataat gttactattc
     5701 gtatgcctga agaggttcca ccaaatatct ctcaagtatt taaagacttg attccattca
     5761 ctgtagcggt tgttctcctt tatggatttg aactcattgt taagggaact cttggtgtaa
     5821 ccgttgcaga atcaatcggt acccttcttg ctcctctctt ctcagctgca gatggttacc
     5881 ttggaattac acttatcttt ggtgcttatg ccttcttctg gtttgttggt atccacggtc
     5941 cttcaattgt cgaaccagca atcgctgcta tcacttatgc caatatcgat gccaacttgc
     6001 atcttatcca agctggacaa catgcagata aagttatcac ttctggtact caaatgttta
     6061 ttgttaccat gggtggaaca ggagctacat tgattgttcc attcttgttc atgtggattt
     6121 gtaaatcaga acgtaaccgt gccatcggac gtgcctcagt tgttccaaca ttctttgggg
     6181 ttaatgagcc aatcttattt ggtgcaccaa tcgtattgaa tccgattttc tttgtaccgt
     6241 tcattttcgc tccgattgtc aacgtttgga tctttaaatt ctttgttgac acattgaaca
     6301 tgaactcatt ctctgccaac cttccatggg ttactcctgg tccgttggga attgtactcg
     6361 gtactaactt ccaagttcta tcatttatct tagccggact cttggtagtt gttgatacta
     6421 tcatttatta cccatttgtt aaggtatacg atgaacaaat tcttgaagaa gaacgttctg
     6481 gtaaaacaaa cgatgctctt aaagaaaaag tagctgcaaa cttcaatact gctaaagcag
     6541 atgccgttct tggaaaagca ggcgttgcta aagaagatgt agcagcaaac aataatatca
     6601 caaaagaaac taatgtcctt gttctttgtg caggtggagg tacaagtggc ttgcttgcca
     6661 atgccttgaa caaagcagca gcagaataca atgttccagt caaagcggca gcgggtagtt
     6721 acggtgctca ccgtgaaatg ttgccagagt ttgacttggt tatcttggct ccgcaggtcg
     6781 cttcaaactt cgatgatatg aaggctgaaa cggataaatt gggcattaaa cttgcaaaaa
     6841 ctgaaggagc tcaatatatc aaattgacac gtgatggaca aggtgctctt gcatttgttc
     6901 aacaacagtt tgattaataa atttgtaaaa gttatttgag acgagattat tctttcagtt
     6961 ccttataata ttgttaacca tatcattgca tttcatttcc ttggtttccg gaactcagaa
     7021 tatcgtctca aatgcttttt ttgaaaggat ttatagttat gactaaaaca cttcctaaag
     7081 attttatttt tggtggtgct acagctgctt atcaagcaga aggtgcgact cacacggatg
     7141 gtaaaggccc agttgcatgg gataagtatc ttgaagacaa ttattggtac acagcagagc
     7201 ctgcaagtga tttttaccat aaatatcctg ttgatttaga attagctgaa aaatatggcg
     7261 ttaatggtat tcgtatttca attgcctggt ctcgtatttt cccaactggc tatggcgaag
     7321 taaatgaaaa aggtgttgaa ttctaccata atctttttgc cgagtgccac aaacgtcatg
     7381 ttgagccatt cgtgactttg caccactttg acacaccaga agttcttcac tcagatggag
     7441 atttcttaaa ccgcgaaaat atagaacact ttgtagatta tgccgctttc tgttttgaag
     7501 agttcccaga agtaaactac tggacaactt tcaatgaaat tggcccaatt ggagatggtc
     7561 aatacttggt tggtaaattc cctccaggca ttcaatatga tcttgccaaa gtcttccaat
     7621 cacaccacaa tatgatggtt tctcatgcac gtgctgtgaa attgtataaa gataaaggtt
     7681 ataaaggcga aattggtgta gtacatgcgt tgccaaccaa atacccttat gatccagaaa
     7741 atccagcaga tgttcgtgcc gctgagttag aagatatcat ccataacaaa tttatcttgg
     7801 atgcaaccta ccttggacac tattcagata aaacgatgga aggggtcaac cacatcctag
     7861 ctgaaaatgg tggagaactt gatcttcgtg atgaagactt ccaagcactt gaagcagcta
     7921 aagatttgaa cgatttcctt ggtatcaact actacatgag tgattggatg caagcctttg
     7981 atggtgagac tgaaatcatt cacaatggta agggtgaaaa aggaagttct aagtaccaga
     8041 ttaaaggtgt tggtcgccga atcgcaccag attatgtgcc tagaacggat tgggactgga
     8101 ttatttatcc tgaaggcttg tatgaccaaa tcatgcgatt gaaaaatgat tatccaaact
     8161 acaagaagat ttacatcact gaaaacggtc ttggatacaa agatgagttt gtagataata
     8221 ccgtttatga tgatggtcgt attgattacg tgaagaaaca cttggaagtt ttatcagacg
     8281 caattgcgga tggtgcaaat gttaaaggtt acttcatctg gtcacttatg gatgtcttct
     8341 catggtcaaa cggatacgaa aaacgttatg gactattcta tgtagacttt gacacgcaag
     8401 aacgctatcc taagaaatca gctcattggt ataagaaagt agcagaaact caagtgatag
     8461 agtaatatta cttgaaaatg taaaaaattg ttatccgata ctttagaaaa agtacaacac
     8521 ttatttagac caggctttga aaaggtatat attgttagtt tcgaagattg tcctatgatt
     8581 cctgagcttg aagctaccct cgttgctaaa atgtggtaaa tggtatgttt taactggaaa
     8641 agagtggatt tgccattctg atttagagtt atcagcattt gaatgggaat ttttacaatc
     8701 actcgatgta gaaatacgtg agactattca cttcgaggta aattatttgc ctttccaata
     8761 ataaaaagaa aggaattagt tgtgactatc gaattaaaaa acgagtatct tactgttcag
     8821 tttaaaacac ttggtggtca actgacttcg attaaagacg aggatgggat agagtatctc
     8881 tggcaagctg atccaaatta ttggaatgga caggcgccta ttctatttcc tatctgtggt
     8941 agtttacgaa atgattgggc tatttataga cctcaagaaa gacctttttt tacaggtctt
     9001 attcgtagac atggttttgt tcgaaaagaa gaatttattc tggaagaagt caatgataat
     9061 agcgtgacct ttagtattaa accaaatgct gagatgcttg ataattacct atatcagttt
     9121 gaactgagag ttgtttatac cttgaatggt aaatcaatta gaaccgaatt tcaggtaacg
     9181 aacttggaaa tggaaaaaac aatgccttac tttattggag cccatccagc attcaatagt
     9241 tctttagtag aaggtgaaaa ctatgaggat tattatattg aatttagtga ggttgagtct
     9301 tgctcgatac caaagagttt tcctgagaca ggattattag atttgcaaga tcggacacct
     9361 tttttggaaa atcaaaaaat tctcgatttg gactactcac ttttttccca tgatgccatt
     9421 acacttgacc agctcaagtc tcgtagcgta acgcttcggt caaggaagtc tggtaaaggt
     9481 cttcgagtag attttgatga ttttccgaac ttgattctgt ggtctacaac taataaaagt
     9541 ccattcattg ctttggaacc ttggagtggt ctctccactt cacttgaaga aggtaatttc
     9601 ttagaagata aaagacaggt aacgaaaatt tcacctcaag aaacttctag aaaaatctat
     9661 gatattacta tccttaatta aaatttgtta ttatttgagt cttcaataag atttgaatac
     9721 tcttaccttg tgatgataga ttggtcttct ccgtgccagt cttacatcgc aaggttttta
     9781 agttttttgt gggtctatgt caactgtagt gggtgacgaa aagctaatat ctaaagacag
     9841 tctggtagta aaaaataaga ttatttacta cttcttgatt taaaataata gtggagccat
     9901 taatttgtgg caaatttaat ttaatatttt gcctcaaatt agccacactt tttatagaac
     9961 taagaggaaa aatgaaattt tcttcctatt aatatagttg tttgaaatct taaatatagc
    10021 gt
//
LOCUS       DAXWOO010000104         9877 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.105, whole genome shotgun sequence.
ACCESSION   DAXWOO010000104 DAXWOO010000000
VERSION     DAXWOO010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.105"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(105..560)
                     /locus_tag="ACGE70_001983"
     CDS             complement(105..560)
                     /locus_tag="ACGE70_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693609.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(714..1922)
                     /locus_tag="ACGE70_001984"
     CDS             complement(714..1922)
                     /locus_tag="ACGE70_001984"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFF8693610.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2034..2366)
                     /locus_tag="ACGE70_001985"
     CDS             complement(2034..2366)
                     /locus_tag="ACGE70_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFF8693611.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2514..3389
                     /locus_tag="ACGE70_001986"
     CDS             2514..3389
                     /locus_tag="ACGE70_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFF8693612.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3471..4052)
                     /locus_tag="ACGE70_001987"
     CDS             complement(3471..4052)
                     /locus_tag="ACGE70_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFF8693613.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4058..5317)
                     /locus_tag="ACGE70_001988"
     CDS             complement(4058..5317)
                     /locus_tag="ACGE70_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFF8693614.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5319..6365)
                     /locus_tag="ACGE70_001989"
     CDS             complement(5319..6365)
                     /locus_tag="ACGE70_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693615.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6600..6875)
                     /locus_tag="ACGE70_001990"
     CDS             complement(6600..6875)
                     /locus_tag="ACGE70_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFF8693616.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7118..8428)
                     /gene="der"
                     /locus_tag="ACGE70_001991"
     CDS             complement(7118..8428)
                     /gene="der"
                     /locus_tag="ACGE70_001991"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFF8693617.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8610..9839)
                     /gene="rpsA"
                     /locus_tag="ACGE70_001992"
     CDS             complement(8610..9839)
                     /gene="rpsA"
                     /locus_tag="ACGE70_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFF8693618.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
BASE COUNT         2749 a         2107 c         1660 g         3361 t
ORIGIN      
        1 cttgtcacag tcccttattc tgaataaacg gtgtggaaga gacagcctat cagtagtaat
       61 aagacacatc tctttcacag cctcattttt gcgtaaaaat cgatttattc gaaaaattcc
      121 ccgtatacat cttctacacg ttgatctgga ttgatcacga tatacaagtt acctgttttt
      181 tcgctgattt ttgttgattg atattgattg tctaatcctt ctgttccttt atctagatat
      241 gggaagtaca caagatcagg tgtgccattt cggtaaagga tttccattcg ctcgatatct
      301 tcgtatttca atgcgcgatt aaacatgccc atttccagtc cgcctaggtt gatatcatgt
      361 gtcgcaactg cgtccccttc atggaagatt tcgattttaa atccagcaca cggatggatt
      421 tcaacaaatt cactgccatt gattcgtcca aaacttgttg tgatttgttt gatccaaaga
      481 tcgccgatat gacggcggtt gatcgtccag gtttctccat ttctaaaata aaattttagc
      541 gctgtcattt ctttactcat taaaaagact cctttatgat gtaatttatt cttgtactac
      601 ctcagtttag cacaaattta tgtaagtggt cacatcttat gttttttctt ctaatgacgt
      661 atctgcccac tttaagtagc caacaaagct gagcagatac agtctagtgt tttttaaatc
      721 aatttttctt tcgataaaaa atccaaaacc tgttttttat cattttttat tctttgttct
      781 acgacggctt tttcagctaa ttgcaaagct tcactaaccc acttaccagg tttttttcca
      841 gtttcttcta gcaagatttt accatctact gctaattcgc ttaatgactt gataggtaaa
      901 cgatcatagg attctcctac ggcatccatc tgaggtgttt cgtcaaaata gatcatcaat
      961 tcttctactt gcagtgcatg ttttttgcct aagaagtaca attcgaatgg ttccaaagct
     1021 ccagtttctc gttgtcgtag tccttggaca agtgcttgaa cgtctttaat catttggtta
     1081 gaacacttcc acgatttcaa aaaggaacga atctcatttt cttttagatc cagttggctg
     1141 atcaataatg cccatgcttg gctctcttct ttcaatggga cggatggtaa ttcagcaaaa
     1201 cgtaaaagtg cttcgccttc ttctttcaaa ccgggacagc aaatgtagca ctctgtttca
     1261 ataaatgcat ggattcccgc ggcccgttca tttcccaaga gcatcttgat gaattccaca
     1321 tttatacgtt ctacagatat tttacctaat agctcgtggt tttctttgat ggcgtagaag
     1381 gtttcttcct ctaatttgaa ccctagttga ctgacaaaac gcaacccgcg catcattcgt
     1441 aaggcatctt catggaaccg ctcatgagga ttgcctacag ctcgcaagat ctgttcttct
     1501 agatcttgca gaccatcgaa caaatcgatg atttctccat cttctttcaa agcaaacgca
     1561 ttgatcgtaa aatctcttct tttcaaatct tctgccaacg aacgaacgaa ttcgacatga
     1621 tctggtctac ggtagtcctg ataagttgat tccgtccgaa atgtggtgat ctcatattgt
     1681 tcatcatttt ctaataccaa cacagttcca tgttcgatcc caatatctat cgtttttgga
     1741 aaaagctgtt tgatttcttc tggaaatgcg cttgttgcga tatccacatc atggattggc
     1801 tggttcagca agatatcacg aacacttcca cctacaaaat aagcttcata tcctgcattc
     1861 tctaattttt tcagaactgg aattgccttt tggtattcca gaggtaattg ttttaatttc
     1921 atttttccaa cctttcctgc gtgtttcttg tcctgagata aagactcagt tgcatggtga
     1981 ctgtttgaca agtccgtttc agagtcttca aggccaggac cacttcatct tgtttactcg
     2041 tccttacgct caaagcgatt atggtctctt tgattgaatt tttccatgtt tttttcaaaa
     2101 acttcagtta agtcgatatt caatgaatta gccatgatca tcgtaacgaa taaaacatca
     2161 ccaagttctt ctgctaccga gttaactggt tcagaagcct ttttgctttt ttctccataa
     2221 gtgtggttga cttctcttgc caattcacct acttcttcag ttaatcgagc catctgagct
     2281 aatggagaga aataccccac cttgaattgc tggatatatt catccacttc ctgctgcata
     2341 ctgtataaag aacgcttttt atccatcttt ttccctccaa aactatctta ccaaaacttt
     2401 aacgcaaatc ctagataaga gcaattgaga aaatcgaatt tccatgctac atttaggaag
     2461 gaatttctaa atggaaattc tgtgaaaata cataattgaa agctggaatt catatggtca
     2521 aacgaaaaac agtaaaagaa gttcttttta tcattatagg aacaagtatt tatgcatttg
     2581 gtcttgttta cttgaacatc gctaatcatt tagcagaagg gggagtctca gggatcacct
     2641 tgatcttaag agctttattt ggtattgatc cagcttatac gactttatta atcaacatcc
     2701 cattgatttt actaggagga aagatcctcg gtaaacgttc cttggcctat actgttctag
     2761 gtacactttc tttgtcattc tttttatgga tctggcaaag ggttccacta gaaatcaatt
     2821 tacagcatga tctactgatc gtttctctac ttgcgggttt agttgcaggt gtaggaagcg
     2881 ggatcgtcta ccgcatgggt ggaacaacag gcggcagtga tattatcgct cggattttag
     2941 aacaaaatta cggcatcact atggggcgtt ccctattggc atttgatatc atcgtcttac
     3001 ttgcttcatt aacctatatc gacttgaaac gtatgatgta tacgttgatt gtttcgtatg
     3061 ttttcagtcg tgtgattgat tcgatcttag atggcggata ttctgcaaaa ggtctacttg
     3121 tcgtctccaa caaaagcgaa gagattgcac cactactgat gaccggtctg caaagaggtg
     3181 tgactttcct ttctggagaa ggcgcattct ctggtaattc aaaaaaaatc atctatatgg
     3241 tcgttagctc tagtgaatta actgaagcta agaggatcgt ccacgagatt gacgaaaaag
     3301 ctttcctttc aatcatcaat gtgcatgaag tggaagggca aggctttact tatttgaagc
     3361 cacagacaag attactgaaa aaactataag aaaaaagaca aagataggct atgaggaaag
     3421 attccacatc cagctgacct ttgtcttttt tagtactata ctatttttaa ctaattgtta
     3481 tgttcactta cccggttact tttcttttgt gtctgcaaaa ggatctttcg gttaacttca
     3541 tcagataatt tcaaaaaagc atccctatct ccatcttcta gtgcttgatc gatttgagca
     3601 taaagcattt ttagttttgc ctcttcttct ttttcttgaa aatatcgttc aattaattca
     3661 gtatcttctt cactgactga agaatcccaa gaagaaaatg gattgtcttc taagatagat
     3721 agatattctc ttgtctgcca agcattttcg aatatacatt ccacatataa aggctttttc
     3781 caattcaaac ggatttcatg aaaaatctgg tctgtgtccg taaattcttt tccctgcaaa
     3841 aacaatttca tgggttcccc ttcaacagat agatcactga tctgcagtcc tcgatccgca
     3901 aaattcgccc cttcaacaaa gtggacattg cttaaaatcg cttcatgatt ggcaagataa
     3961 ttcaagatcc aaaaaacttc tcttcggcta aaagatacat gattgaccat ccacgttaaa
     4021 aaattcttct tttcccgtac atcaattagc atttttctca cctctctgtt aggtcatcta
     4081 ataatgacag tgcctccata tctcctggtt caagtgttaa ataataagtc aataattctt
     4141 gcgctctttt cagctgccct tcttctctta agaaaaaggc atactctttc aagaaatctg
     4201 gttcatgtga tagttcatga taagcctgtt catagtgtac agctgcaacg gtaaagtctt
     4261 ctaattcatt ataagcatgg gctaggttcc attctgcata aggattagca gtttcttcca
     4321 tctgattgat cacttgtatc acatcttcat atctttcatc atccaagtaa agattactta
     4381 aagtaagtaa tgtttcatct tgtttgtctc ctacttctaa agctttcagt aaaagttctt
     4441 ctgctcgctg tctatcatgc agacggaaag cattttccga ggccaggtga taaagttcga
     4501 cttggtaagg attttctttg attccttctt ctaacactgt ttgtgcttct tcaatcattt
     4561 cttcttcttg aagagcttgt cctagatata gatataacga ttgataatgc ggattaactt
     4621 ctcgaagttc ttccagatag ttgattgcct gctgattttc tttcaattga agattcgtaa
     4681 aagctagctg gaacagtcga tcatctgtcc tttcttcttt cagtgcttct tctaaaggta
     4741 cgatagctgc ttcgaattct ccttgcatac ttaaggattg tcctaaacgt tcctgcatcg
     4801 agattcctga aatttcattt atcccgcttg ccaacaatgt ttcataaacc actgctgcct
     4861 ctttgaaacg atcaactgaa aaatacaatt cagctagcgc aaactggatc aatggttcat
     4921 caggaagcaa cccagctgct tctttcaatt ttgcttcact cacttcaggg attccgataa
     4981 cttggtacaa atcagctatc gccaataagc tttgcggata gtaatcactt tgcttatcca
     5041 ctttttccag gtattcaaaa gctaagtcga tctcattatc ttcgattgca atttctgcta
     5101 acgggatgtt cagtccgtca ttttctgggt attggttgat cagttgttca aaaatctgtt
     5161 ttgcttcgat caaaaagcca atagccagca attcttcgcc tagttcagct aatatatctg
     5221 ctgggtcttc ttttaatgct ttttctaaaa agatttgtgc ttgtgacaag tcttcatctg
     5281 ataaagcttg caacattttt tcgctatttg tattcatttc aattccctct tatctcactg
     5341 acgatttgtt gaaataccgt cattgttctc aataattctt tttcgtttgg atgttggtat
     5401 ccgtctatta tacctatttt ttctaaaaca agtatttttt tgcttttttc tgccaaaaaa
     5461 agaacttttt ccaaatactc catctttgaa atctgttcca gccagtggat tggatagcca
     5521 agcaagtcca gccacacaaa aaagtttttt agttggaaat caaaaggata tgcttggaga
     5581 ttccacacga aatgaaacat cattcctaaa aattttttca tactagcaga aagtagatgg
     5641 ccattttcgg tcagataaaa agcttgttca aacagtttac cataatttga caggttttct
     5701 gcctctaatt gataaaaata gatcatttct tcgatcatcc cggcaaacgg ccgagtcgtt
     5761 aattgctttt tatttggata gtttttatac agattttgca aaaaacgatg atcacaaatc
     5821 attccgcaac agataaaagt caaaaagtca atctgctttc cgtccacctg ccgctcgctg
     5881 attgtttgat cataaacgat gcgttctggc agattgatgg tttgcagcac tacacgattt
     5941 ggtagctgca aaatcgtccg ctgcggtgtt aatgctcggc ttagagaacg gatcgataca
     6001 gggatcatcc atagtgatga atcaagggtt gtatgttttt gaaaaaaacc tgccagatcc
     6061 atgatccctt catttccaaa tccgatgata aacatggatt gattttcggg atagcgtttg
     6121 acgaaatcaa gcaggttctt taattctgtt aaatgattgc attgagtgtt ggcacaaata
     6181 taccagtcat tgtttgcttg atttgcaaat aacgggttga tcttatctgc aaacaaatca
     6241 taatagcgct gattcgttaa aaataagatt ggctgatcaa tggaaagttc tcttagctga
     6301 ctgactagcg tttctccgta aaacactttc gtcaacgcat cttctacttg ataatcaagt
     6361 tccatacgca tccctcctct tcttattttt attttaccat tagtaagcaa attctagaaa
     6421 agataagagc aaagctcacc tcataacttg gaaataaata aaaattaaat aaaatacatg
     6481 tgttttctat gtatgacata cggattcgga atctctgtct cttatttatt ccttttctgc
     6541 taggaaataa aaagaaaagc acttgtagcc ctagagccac aagcgctttt tcaactgtct
     6601 tatttaacag cgtcttttaa tgctttacct ggtttgaatg caggtacttt gctagctgga
     6661 atttcgattt cttcaccagt ttgtgggtta cgacctttgc gagctgcgcg ttcgcgaact
     6721 tcaaagttac caaaaccgat taattgaact ttttcgccgt tagctaaagc atcttgaata
     6781 gttgaaaata cagcatctac tgctgcagtt gcatcttttt tagttaaatc agtagctgaa
     6841 gcaacttttt cgattaattc tgctttgttt gccatggata gattcacctc ctccacaaga
     6901 gatatgatag aaatatcatt tagttaaaac tttctgagtg gtccagaagt ttaaaaagaa
     6961 atattgatta acttcaatat tctgttatca cgatatcata tgaaccgctt aatagcaagg
     7021 tttttaccaa ttttcacatg ctttttttag tcatagaaac gtaacaaacg aaactgtctt
     7081 tttcttttga aaaaaaaacg tcgatatggt aaaatgccta ttttctccta cgagggataa
     7141 ttcggatgga cgtcccttca aaagtaaatg cttttctgat ttgattttct aagaaacgtt
     7201 cataagaaaa atgcatcaat tcctcttcat tgacaaaaac gacaaatgta ggtggtttga
     7261 cagcgacttg tgtagcataa aaaatcttca ggcgttttcc tttatctgtt ggtgttggat
     7321 tgattgctac agcatccata atgatatcat tcaacacagc cgaagagata cgaagattct
     7381 ggttcatact tacttgttcg atcatttcag gcagacgttc caaccgttgt ttagtcactg
     7441 cagaaacaaa aacgattgga gcgtaatcaa gataccggaa ttcatcacga atctcttgtt
     7501 cgaaatcacg cattgtattg gtttctttct ttacaagatc ccatttattc acgacgataa
     7561 taattccacg accagcttca tgggcgtaac ccgctactcg cttgtcttgt tcccgaatcc
     7621 cttcttcagc attcagtacc atcaaaacga tatcagaacg gtcaatggca cgcattgcac
     7681 gcatcacgct gtatttttca gtattttcgt atacttttcc gcgtttacgc ataccagctg
     7741 tatctatcat taagaatttc tgaccacttt cagaaacgaa gtgggtgtcg attgcgtctc
     7801 gggtcgttcc ttcaatatcc gaaacaatta cacgttcttc tcctaaaatc gcattgatca
     7861 aagaggattt accaacattt gggcgaccaa tcaaactaaa cttaatcgtg tcgtcgtcct
     7921 cttcttcagt ctcttcagag aaatacttca ctgcttcatc taaaatatca ccaatcccta
     7981 gtccatggct tcctgaaaca ggataaggat cgcctagccc taaggaataa aattcataga
     8041 tatcgtttct catttctgga ttatctactt tattgactgc taaaatcact ggtttattac
     8101 ttctgtataa aattttcgca accatctcat ctgcgtctgt cacgccttcg cgtccgctga
     8161 ctacacaaat gattacatct gcttcttcaa tcgcgatttc tgcttgatgc ttgatctgat
     8221 ccataaaagg ttcatcgcca agatcaattc cgcctgtgtc gatcacgctg aattctcgac
     8281 ctaaccattc accttttgca taaatacgat cacgagttac tccaggagta tcttcgacaa
     8341 tcgagatacg ctcaccagcg atgcggttaa aaatcgtaga cttaccgacg ttcgggcggc
     8401 caacgattgc aattgttgga tttgccatat tatttcctcc tcacaagttt ccaatctcac
     8461 ttagtttaac gaatgcattg ccaactttca agcatagatt gtcaattaaa cgaagtattt
     8521 ttattcctta ttgaaatact aatcgctttt caaaagaaaa actgagacaa aagcagctgc
     8581 tttcatctca gtcacagata caaaatcaat tatttttcag aatcatcggt tgctttttct
     8641 tctacttctt ctttcaacgc atcacctaaa atgtctccca tagtaaatcc agtattttct
     8701 tctggtaatt cataagactc aacatttttt ggttcttctt ctgaagctgg tttttcttct
     8761 aaagctttga tactcaatgc aatacgatgt tcttctggat ggatctctaa tactttcact
     8821 ttcacttcgt caccttcatg aagcacttca tgtggtgtcg caatatgttt atgtgaaatt
     8881 tgagagatat ggactagccc ttctactcca gggaacactt caacaaaggc accgaagctt
     8941 gtcaaacgtt taactgttcc gtctaatact gtgccaactg gagcttttgt atcgatttct
     9001 gaccaaggtc caggctgtgt gtctttgatt gacaaggaaa cacgttcttg ttcaggattg
     9061 atagacaaca ctttgacatt tacatcatca ttgacagcca atacatcact tggttttgct
     9121 acatgactat gtgaaatctc tgagacatgg actagaccgt caattccgcc taggtcgaca
     9181 aatgcgccaa aatcagtaag acgtgcaaca cgtccactga gaacatctcc ctcatggata
     9241 gttgaaagaa tttctttttt cttttcttct ctttcgtttt ccactaccgc tttatgcgat
     9301 aagatcaaac gattttctga tggttcgatt tcgatgattt taaagctcaa tgttttgcct
     9361 ttatagtctg aaaagtctgc aacaaaatga tcttccacca ttgatgcagg gacaaaacca
     9421 cgtacaccga cgtcaacgac taaaccgcct ttgacaacat ttgtaacagg tgcttcgatg
     9481 accttgccct cttggaaatc tctttcgata tcttcccaaa cttttttagc atctaaacgg
     9541 cgttttgaaa gtaggtagct gccgttttct ttatctttgc cgatcgtact gataacgact
     9601 aaatctaata catcaccgac ttttactact tcgctgatat cttctaccgg caaagttgat
     9661 aattcttttg ccggaacgac tccttcaact ccagcccctt cgattccgac aatcacttgt
     9721 ttgtcttcaa tgactaaaac ttcgccctta acaacatcgc caacttttac ttcttgtacg
     9781 cttttcattg cgtcttccat tgactggttg ttctcttgat tttgatcaaa ttctgtcata
     9841 acacttgtcc tcctaaccaa cattctgccg taagtac
//
LOCUS       DAXWOO010000105         9834 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.106, whole genome shotgun sequence.
ACCESSION   DAXWOO010000105 DAXWOO010000000
VERSION     DAXWOO010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9834
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.106"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(7..213)
                     /locus_tag="ACGE70_001993"
     CDS             complement(7..213)
                     /locus_tag="ACGE70_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693619.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(229..843)
                     /locus_tag="ACGE70_001994"
     CDS             complement(229..843)
                     /locus_tag="ACGE70_001994"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFF8693620.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(990..1673)
                     /locus_tag="ACGE70_001995"
     CDS             complement(990..1673)
                     /locus_tag="ACGE70_001995"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFF8693621.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(1782..2240)
                     /locus_tag="ACGE70_001996"
     CDS             complement(1782..2240)
                     /locus_tag="ACGE70_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFF8693622.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2324..4087)
                     /locus_tag="ACGE70_001997"
     CDS             complement(2324..4087)
                     /locus_tag="ACGE70_001997"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFF8693623.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4308..5768)
                     /locus_tag="ACGE70_001998"
     CDS             complement(4308..5768)
                     /locus_tag="ACGE70_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693624.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(5976..6902)
                     /gene="glsA"
                     /locus_tag="ACGE70_001999"
     CDS             complement(5976..6902)
                     /gene="glsA"
                     /locus_tag="ACGE70_001999"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFF8693625.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(6925..8352)
                     /locus_tag="ACGE70_002000"
     CDS             complement(6925..8352)
                     /locus_tag="ACGE70_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFF8693626.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8383..9597)
                     /locus_tag="ACGE70_002001"
     CDS             complement(8383..9597)
                     /locus_tag="ACGE70_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFF8693627.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         2918 a         2041 c         1628 g         3247 t
ORIGIN      
        1 aaatatttat ttgttaaagt aattaaacaa tccgctaatc gtttgatctg gcatttctat
       61 ttccttacca tctaaagtaa cagtagtaac gacaaaatgg tcttgcaatg gcggaacttc
      121 taagtactgc ccttgcagaa aatcattcac tgtttcatat tcttttttca tttcttttga
      181 atctgtcgat ggtttatagc ttatttttaa cataactagt gctccttttt attcgggacg
      241 tgcaatcgtg aaaatttctc ctattgctgc ataatcattt cctgccaaac ggcttacagc
      301 attcaatttt cttggatcga tccttcctgt cttatcatca tagactgttt catttaaatg
      361 atatttctgt acttctagca aaaataaatc agctgttact ttatttttat tccgtatctc
      421 gacatgttca tataaggatg tttcaaaacg aacttttgct tctgtcaatg ctggtactga
      481 aatgattact gaatcagtta aagtcaagtc agtcaggtac agttcgcttt cctcattagg
      541 cagattggct gctgttttat ttacttcttc tacgttgtcc tcatcaagga tatggataac
      601 agcttctctt gtctcaagaa tattctttgc tgtgtctttt actgcatctt gttttctctg
      661 gatcgccaag gataagatgg gtcgattcga actgacgata ttgaagtaac tgaagggtgc
      721 gatattcact atatcttttg ctgatttagt cgttactact gctactggtc tagggatgac
      781 tgaaccaatc agcaacttgt aattttcacg ttctgtcaat tctgaaggac tccgttcaaa
      841 catggtttcg ccttctttct ataaaattcg ttaaaaagca gatcttgaag acaaaatgaa
      901 aagtaatgct tttttcatct tcttagaatc tgctttaaac tatctatcaa tttttctttt
      961 ttgtcaagaa tctgtgccgt gcgtgttttt tagttatttg ctttacttgc ggcttcgaca
     1021 tatcttttag tgatcaattg cggacgggtg ataatagatc caactacgac tgaaaaagca
     1081 cctaattcat atgctttctc tacttgttca ggagagtcag tatgtccttc cataattact
     1141 gggatatcga cttgttctat gatttgattc aataaatcaa aatcaggtgt ctgcgtcttt
     1201 cgtgaatatt ctgtatagcc tgctaaagtc gtagacaaaa gatcaaaacc atcttcttgc
     1261 gcttgaagcg cttcctcaaa agtcgaaata tctgccatag ctaaccgacc gtgttcgtga
     1321 atcatttgga caagttcagc agttgttttt tctgtcggac gtttacgaga agttgcatcc
     1381 agtgcaatga tttctgcttg cgtctccaaa agttcttcca cttctttttc tgtcgcagta
     1441 ataaacactt ctgacccatc gtaatctctt ttgataatcc ctattaccgg taaatcaacg
     1501 attcgcttga tttcattgat atcttctgtc gtatttgctc gaataccaac tgctccgcct
     1561 tcttttgcag ccaaagccat tctccccata ataaaagaac tgtgtagcgg ctcgttttcc
     1621 aaagcttgac aagaaacgac gactccgcct ttgatttgtt ccagtgtatc cacaatatct
     1681 cctccagtaa tgttatataa aaatacagct tcatataaaa atgtaaccct tttctattca
     1741 ggagacaata atgagctatt tctttcagca gacctttctt actaaaagaa aattacttca
     1801 aattcttcgt atacgaccag cttctcttct gtaaattcta tgttcaacgc ttctttataa
     1861 gcatgtttaa aaaactcagc atggactttc cgccaataag ctaatgtccg atccccttct
     1921 ccttctttcc aagcatgctc tttcgtgact tcactaaaag gcaagatggt cacatttata
     1981 tttttgatga tacaaactgg ttcatttttt ccattcagga ccacgtctaa ttgaccaact
     2041 tttggtaaag gctcattctc ttgtttatac agatcgtaac cacttgatgt accagtcttt
     2101 atctttttta caactagttc gctcaactcg tctgccattt tttctgtatc cccaaatgaa
     2161 aagaaaccta atttttctga ttgataacta ggatattttt cttggaactt tttgacatat
     2221 tcttctactt gctgatacat aacatccacc cctttgcttt ttataatgaa aaaaccatcc
     2281 agatactagt ataaacaatt atctggatgg tgagcataat gaattattga atattcaaga
     2341 cacgcagact atcttcaacg atttgttttg gtgttaaatg ataacgttca taaagctgcc
     2401 ctacaggaac gctatccgtg aattctttct ctgcaccaaa atttaatacg ttcattgaag
     2461 aatcaccata ataacgactg atattttcgc caaatcctcc tgacaaacta ccatcttcta
     2521 atgtgatgac taattcgtgt gtttctttta atttatctaa tgtttctacg tcattttctg
     2581 tgatcgttcc tggattgatc aacgttgctt cgattccttt ttttctaaaa gcttttactg
     2641 tttcttttcc taattcaaga aaaccgccta aagctaaaat tgctactttt cctccattat
     2701 ttactgtttg ataggctgga atactataat catttgcgat agctgtacca tgttctacac
     2761 catgtgtagg aattcggatt gctacaggct gcgctgtttg ttcgaacgcc caatctagca
     2821 tagagatcat atcctctttt gttgctggag caagatattt gatgttcggg atacttgata
     2881 agtaaggaat gtcaaatgtt ccttggtgtg ttcggtcact agctgagatc gttccgccac
     2941 cgactaaaat aatcactgga ttctcgttga tcgctaaatc atgtgataat tgatcatatg
     3001 cccgttgcaa gaaagtactt gattggaaga cgactggacg tactccctga gaagccaaag
     3061 cagaagcgaa agtgatagag tgctgttcag cgatccccac gtcaaaataa tgatctggat
     3121 gtttttcttg gaatgttttc agtccaaagg cgcctggtat cgctgcattg actgcggcaa
     3181 taggagctgt acttgtttgc attttcttat ccaaataatc catgatgatc atattaaatg
     3241 tttctccaga agttacttgc tttgcctccc cggtggacag atcaaatggt acatgccaat
     3301 gatatttcat tttattatcg attgccggct gataaccgcg acctttttcc gtatggacgt
     3361 gaagtacaat cggatgatca atatctttta cttcttcaaa taaacggatc accgtttcca
     3421 gatcgtttcc attatccacg taacgataat caaatcccat tgctttaaat aggttattgc
     3481 tgcttttacc gttggtttga cgaagctcag ctaagttttt atataaactg ccatgattct
     3541 catcaataga catctcatta tcgtttacta atacgattag attggattgt aaaacaccac
     3601 cattgttcaa tccttcaaaa gctaagccac cacttaggga gccgtcaccg ataaatgcta
     3661 ccacatttcc tgattctttc ttcagatctc tcgctttagc taaacctgtt gctaagctga
     3721 tagatgtaga tgtgtgcccc actgtaaaat agtcatgttt actttcatgt tggttggaat
     3781 atccgctcac ttcacggtaa tgtccttttt caaaaccatt ttttcgacca gttaaaagct
     3841 tatgaccata agactgatgg gaaacatccc aaacgatttt atcctttggt gaatcaaata
     3901 cataatgaaa cgcaatcgtt aattctacta cacctagatt cggaccaacg tgcccaccta
     3961 catcgctgtc cttttgcaag attttttggc gcatctcttc tgccaactgt ttcatttcct
     4021 caacagataa ttttttgaca tctgcggggc tattgattgc atccaataca tgtaatactg
     4081 tactcataat acattctctc ctccgtacta tcttctttta atgaaaatag atttgttaca
     4141 ctttatctat tctactactt agagttgact ctaagtcaat agcattttaa aaaaatttca
     4201 gagaaagctt gttatataag tagatcttcg cacgaatcag gactcaaata aaaaccttct
     4261 acagacaatt aaaaagcttt gtagaaggtc tcctaattaa ggtctcatca agaaatatgt
     4321 ttttttaata ggtgaagttc ctgagcaata tgatacaaat ggatgtttgc accttttaat
     4381 gagattggat agatctttgt cttttcagtt tctaatccat tttgatagct agcagcatcg
     4441 cacaaaacca ctcttttttt cgcacataga cattcatcag gcaattcttc taatgaacga
     4501 aataaaatag gatttatttc agcgatgcct tcttccgcat acaaatgaaa ttgggttcga
     4561 taaaaaaatt gtttgactga actttctgaa acaacaaaaa tttctatttc ctgtctcccc
     4621 gcttgttcac tgttgatcaa agaaagactt ttgacgaatt ttcgaatgaa attttcattc
     4681 catcttagtt ggatttgatt tttctttttc cactcttcca atatttctac aatttttccg
     4741 tacagaagtt gttctttttc tgatagaaga tagatccttt ccggtaaaaa tacttgtcca
     4801 tctgcccaca acgtacggac aaattgtaaa aaggattgct tgaaaataaa attttctgca
     4861 tcgtcgattt cagcgatact aataatctgt aaaatcaatt cattcagtga taggtcattg
     4921 agaacaaaat ttttataaac aacttcgaaa tcttttctta acagctccat gttctcattg
     4981 gtataatttc tactgttgaa caaagaaaaa atataataga catcttcttc ttgtaagtct
     5041 agaattcctt tttgctcttc caatccttga cagactaatt gaaataacgg cttcgtttct
     5101 gcgcttttct tatcttcttc tttcagtcca aggggaaatg cctttcttcc gattgcgatc
     5161 tctattcctc gtatgataaa ggacttttca tcttccgaat atcttctttt aaaaaaatga
     5221 gtctctacat aatcaatcaa ctcgttacaa gcttcttcta catacggatt tttattttca
     5281 tagccttccc aattgacata acgtactaaa gctagcaaga tgttccgcga gtcaaattct
     5341 gggataatga tttctttatt tttcaaataa tcattttctt taaaaaaatc caatacgatt
     5401 tttttgattg tatatacttt gctcaaagaa ataaattcaa gatcagccag ttctgtactc
     5461 gtgaatatac cattaaaata ataacttaaa aactttaaaa aataagattg accgtacaga
     5521 aactgtgtga gctcagaaag agcaatacca tgtttgaata cgagtttata cttatgcttc
     5581 tctcttactt gttcttttat acaatattct agacactcgg ctagactttc tagatcgtga
     5641 aggatcgttg gtgtagtcac ccttagagaa atagctgttt gttcgatgtc tatttcttgg
     5701 aattcaaata attgttcaca cagatacact tttctaaata tatttcgctc gatgtaattt
     5761 tccagcattc ctgatacacg cctccagttt ttcttatgat tttatttaat catatctgta
     5821 aatcgtattt ttgtttcctt tttatttaga aaagcccttt caaaaaccaa taaaaaaaga
     5881 aactgggaca aaagtcaaac aagctcacaa tttcagcctc attgtaggta gctttgagcc
     5941 gtttggactt ttaccccagc ctctttcaag aatggttatt caaagatgtc agcgtctagt
     6001 tctttgacta catctttcaa caattggata ccagctgcgc tgttaccaaa tggatctagt
     6061 gccgggctga agacacctat gccgtaacga tttggcacag ctgccatcaa cccgccgcct
     6121 acaccgcttt ttgcaggtac accaacatgg acggaaaact ctcctgattc atcatacaaa
     6181 ccagcagttg tcataattga tttgacgatt gtagcacttt cttcagtaat gatttgtttg
     6241 ccattccaag gagcaattcc tttatttgcc agcacagcag ctaattttgc tatgcctgta
     6301 gcggtgacat tgagggagca ttgtctgaaa taagcatcga gtatctccgg tacgtctcct
     6361 tcgatcattt gattaccttt catatagtaa gcaagagagc gattgatatc acctgttctt
     6421 gattcagaat gatagatttc ttcattcaat gtgatattag gatcatcaca gatctctttc
     6481 ataaatgaca agattcgctc aaatttttca tctgcatctt taccggcaat caacgaggtt
     6541 gttgcaatcg ctcctgcatt aacaaaagga ttcattggat gtttgcgatg attgatttcc
     6601 atattcatga tagaattgaa ggcaaaaccg gttggttccg tgttgattgt ttgaaagact
     6661 ttttctattc cgttgtcttt gattgctaat aacaatacgg gaacttttga catgctttcg
     6721 atggcaaagc gcacatcggc gtctcctccc tcaatcagtt gttttgtatc caaatcataa
     6781 atcgccatac ctaattgttt gggatccact tcagctaatg ctggaatgta atccgccact
     6841 tgtcctgttt gataatagtg gaaatttttt ccaatcaatt gttctaattc ttttacttcc
     6901 atttgaataa ccagctttca ttttttattt ttctgcatgc tggaatttat gacggcatgc
     6961 gtagatcgta aatggaataa ctaagacgat aagataagaa acaatcagaa tcgtttcata
     7021 aatcccatgc tgtccagctg cgatactagc aggtggaaca aatgagatac aaaaagcaaa
     7081 aagtgaagtc aaaagaccta aacaagcaac gattactttt acaacttttc cgcctggaac
     7141 ctgataggct ctttcaagac tgtcatcttt aaaaaccagc ttcaaatagc ccgcgaaaaa
     7201 cataaagtac aacatcaggt agattacaac agtcaatgac atcgctgcaa gaaatgaaag
     7261 attattcccc ccgccgctta atgtaaagac ggctgcccag attgttgcaa cgatcccatt
     7321 tgcgatcatc aaatgagcag gaacaccgtg tgtgttttct tttgcaaaat attcaggaag
     7381 taatccttct tctgctgcta cgtgaagtgc ttttgaagga gaggttaccc aagaagcaat
     7441 ttctccgatg acacctaaac aaacaagaat cgcaagaatt ttcgcaatcc atgcaagatt
     7501 tggatcaatg tgtcttaata ggaattctaa cgattgaatc acccctgtat taagagacaa
     7561 accatgttga ggtactgtag tagcgacggt caatccgcca aaagtatcta aaatgattgc
     7621 cagaataacc aatacaaaca ttgctaatgg ataatccttt ttagggtttt ccatttcatt
     7681 tgcatgactg gcagatgttt ctacgcccat atatgctaaa ataaaggaaa caaaaacaac
     7741 cagcgtaggc aatttcgaaa aatcaggaat caatgttttc atagacagta tcgtttgtag
     7801 tggagcgcct gaagcgacat aatggatccc taagccaaac aagactaatg ctggcaacaa
     7861 gataccagct acaaaaccga tccgagcaat caatgaagtg tatttggttc cttttaactg
     7921 tgagagtaaa atagcccaga aaataatcaa taggattgcc aatttcagat atttattgtt
     7981 ttgtatagca ccaatacccg ttgcatagga taatgcacca gttaagaaat acagcatggt
     8041 caagtagcca actgttatct caaaccattg gaaaaagatt gcggcaaaac cgaatcgttt
     8101 tcccaatgtg cgactgaccc aagtatagac accgcctttt tcccatcctt tcaccgttgc
     8161 catctctgct gcgcacaatg ccacagggat aaaccatagc aaaccgccta ataacaagaa
     8221 aaatactaag ctgaagcctg aggtcgcgaa tgtgggatat tcatataatg aaacgaccat
     8281 tgagatggtc atagccaaaa agccaaagaa cgttaatttt tttgcaggaa ttttcgtttt
     8341 gctttgttgc atgaatcttc ctcttttcta tttgaatttg tttcaattga gtttgttgcc
     8401 aaagaagaaa aagtccaatc cttcttcttg ttcttctttt gtagtagtga tcgttgtaaa
     8461 gtgcatcgcc gattttaaaa tcactgttgt cagcacatag gcatacacgg cagtaatgat
     8521 gacaccaatc acttgaatcc ctaagaagta ccagctgctt ggattcgtta gtaaatggct
     8581 gttagatgca aagatcccta tcagaatcgt acctgtgaat ccgcccatgc catgaacacc
     8641 ccacacatca agtgcatcat cccattgttt ctttttacga aaatcaacag cagcatgaca
     8701 gacgataccc gcaatgatcc caatcagcat ggcacttttt gcttctacat aaccggcaca
     8761 aggcgtaatg gctgccaatc cagcaacgga tcctgttagt acgtcgacaa aatcaaagcc
     8821 atttcctttt acttttgcaa tgatcagcca agtaatcatc gcaaatgcaa gagcgatgaa
     8881 tgttgaacca aatgcattga ctgcttgttc atctgctcga agtgcaccac cggaattaaa
     8941 accgaaccag ccaaaccaaa gcagtcctgt accgatagca gctgccataa gactgctatg
     9001 ctgacttctt tctcctaaag gaatctttct ttgaccaagt gtgaggatac atgctaaaga
     9061 gccgaatcca gctgtcgtgt ggatgactgc tccaccagcg aaatctctaa atcccatctg
     9121 atccaaaaat ccgccacccc atatccagtg gcaaacaggg aaataaatca gcagattcca
     9181 aaaaataaca agtataatat agccttttaa attcaatctc cctgcaaaag ctcctgtcat
     9241 caaaggaacg gtaatcacga taaacatcag ttggtaaaga aagaacatta aaaatggaat
     9301 cgttgcacca tgaaacatgt tggggaagct tgtaacatgt ctcatcaaaa agaaatctgc
     9361 cgggtttcca ataacaccgc cgatatcccg tccaaaggcc aatccgaatc caccaaatac
     9421 ccacataatc gttgttatcc caacggagag aaaagattga gacatgatcg ttagtatatt
     9481 attcttccgt gctaatccgc cgtaaaaaaa agctagacca ggagtcatca ggcaaaccat
     9541 tgctgtacat aaaatcatga atgctgtatc tcctgtattg ataggtaatg tattcatttt
     9601 tttcgcctcc tttctacgat tacgagtata aaagaaggtg tagttgtttt tttcttctgt
     9661 ttttttgaat taatacttgc atttataaaa gacagacaat taataaaagc ttgatgtgtt
     9721 gtgctctttt tcgtttttca cacaaataaa gacaaaaaat aaacggtctt ctttttatag
     9781 aaaaccgttt attttcgtct ttttttattt ttaaagatat tttttcataa ggaa
//
LOCUS       DAXWOO010000106         9698 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.107, whole genome shotgun sequence.
ACCESSION   DAXWOO010000106 DAXWOO010000000
VERSION     DAXWOO010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9698
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.107"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(174..1157)
                     /locus_tag="ACGE70_002002"
     CDS             complement(174..1157)
                     /locus_tag="ACGE70_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFF8693628.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(1162..1809)
                     /locus_tag="ACGE70_002003"
     CDS             complement(1162..1809)
                     /locus_tag="ACGE70_002003"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFF8693629.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(1894..2526)
                     /locus_tag="ACGE70_002004"
     CDS             complement(1894..2526)
                     /locus_tag="ACGE70_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFF8693630.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(2542..3093)
                     /gene="coaC"
                     /locus_tag="ACGE70_002005"
     CDS             complement(2542..3093)
                     /gene="coaC"
                     /locus_tag="ACGE70_002005"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFF8693631.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(3090..3827)
                     /locus_tag="ACGE70_002006"
     CDS             complement(3090..3827)
                     /locus_tag="ACGE70_002006"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFF8693632.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(3824..4813)
                     /locus_tag="ACGE70_002007"
     CDS             complement(3824..4813)
                     /locus_tag="ACGE70_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFF8693633.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            5071..5613
                     /locus_tag="ACGE70_002008"
     CDS             5071..5613
                     /locus_tag="ACGE70_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFF8693634.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            5630..6715
                     /locus_tag="ACGE70_002009"
     CDS             5630..6715
                     /locus_tag="ACGE70_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8693635.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            6727..7533
                     /locus_tag="ACGE70_002010"
     CDS             6727..7533
                     /locus_tag="ACGE70_002010"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8693636.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            7534..8361
                     /locus_tag="ACGE70_002011"
     CDS             7534..8361
                     /locus_tag="ACGE70_002011"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8693637.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            8358..9431
                     /locus_tag="ACGE70_002012"
     CDS             8358..9431
                     /locus_tag="ACGE70_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFF8693638.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
BASE COUNT         2926 a         1795 c         1981 g         2996 t
ORIGIN      
        1 taaagaacac ttttgtctca cgctctaaac acgtataaac agcggaagca gaagaaattc
       61 cttcggaaat aagctgaaat tcacaaaaat ttgaaaagca attttcgtga atttcttctt
      121 atttcttgga gttaaacgct tctgtcccag cctctttttt gatcagcatg attttattca
      181 gttatcttca aaatgatttt tccgatgttt ttgtttgctt ccatatattc atgcgcttgt
      241 ttgactgcgc tgaattggaa tgtacggtca atgattggcc gaatcttgcc attttctaga
      301 aaaggcccag ctttttgcat aaaagtttgt gcgagctctg ctttatactg atcacttcta
      361 ggtgtcaata atgtaccaag cagttggaca cgtttcccaa tcaatacacc aaggtctatc
      421 tcaggcacga tggtcccacc tagcatacca atcaagaccc aacgtccatc aacggctata
      481 gaagctaaat tcttttccca gtaagaagcg ccgatgaaat ccaaaataac atcgactccc
      541 cgaccttcag tgatttttcg tacctcttct tgaaagtctt gttttttata gttgatcaat
      601 tcatctgcac caagttcttt acaaaaggcg agtttttctt ctgatcctgc agtaacgatg
      661 attttcgcgt gagaaagctg tctagctaat tggatcgctg ctgttcccac accacttgct
      721 cctgcatgga tcaagacagt ttcgccttct tgtaattttc caagccagta aagtgtttga
      781 taagcagtca aaaaaacttc tgagattcca gcagcagaaa catagtctag cgtatcaggg
      841 agaaggattg cacgatcggc aggcatcaca gcatattcgg cataaccacc tagattgact
      901 aatccgtata cttttgtccc aggagtaaaa gtggagatat cacttttatt ctcaactacc
      961 tctccagcta cttcgactcc caaaataggg tagggaggtt tcaaagagac tgattctctt
     1021 ttcataatat ctgttcgatt gacagctgct gtatggactt tgatcagtaa ttcgccttgt
     1081 tttggtttag gaacaggcac ttcaatttgt tctaatacat ctacagaacc aggatgcttg
     1141 attgaaatgg cacgcatgga tctattcctc ctttgcccat tcttggaatg ctgtattcaa
     1201 ctctttgcta ccttcagaaa gtactagagt cgtacgagaa atatccgttt cttccactag
     1261 agcggcaagt acagcagcaa ctgtagagcg agtgatcgtt tttgatgcct tctcattagg
     1321 cttagttaat tgaatggaag taacttcttc atcgttagtc aatgtgactg gttgaagaat
     1381 gacaaaatct aagttagttc gtttcagcca ttcatctgca taatgtttag taatataata
     1441 atctgtcatg gactcaggcc attttgctgg ttcatctgca taaacagcac tgaccatcag
     1501 atagcgagag acatgcgcat tttcagctgc gatcactgtt ttgattgcgc catccaagtc
     1561 tacttgcaga agattctttc cttgagagcc ggcagcaaaa atcaatacat cgatgccttt
     1621 gaatgcttcg gtcatttttt ccacggacca agttaaatca aaagagacat acgaaacatt
     1681 ctcttttttt actaatgttt gggaagcaac atctctcacc cctgcaaaaa tttcatggga
     1741 acttggagct aaatctttta tcaggtgtcg gccaatttgt ccgtttgcac ctacaacaaa
     1801 aattttcatt tggatcatcc tttcgacgtc aaatctttcc attcaatagt aacaccaaaa
     1861 gagaaaaaaa tcagagatcg gctacgatga ctatcagtta gtaagagaag ggtgcttgaa
     1921 gtatcttaac aagacattgc atactgctgc agtcgcaaga cctgcgacga ttccttcagg
     1981 tataccactt gtaaaaataa ccgctagaat agctgaataa acagcattta aatttgttcc
     2041 gatgactttg gcataatctt gctggaataa aaagtagatc atattcatca ctaaaatcgt
     2101 attggtcaat gaaccggaaa gtccagctaa gaataaagaa acggaatctg ctttttgttt
     2161 cattagtttg cgcagacctt tataaacgaa ataaggaacc accccaatca agatgcgagg
     2221 aataaacgcg atcagcaagg ctttccagct tccatgttct gttccgatga ctgggataaa
     2281 cggagaaaag acaaaagaca aaggtgtttg gataactgtg ctacggatca tgctgatcag
     2341 tccaaatgta ccgccaagaa aagcacctag tcttggtcct aaaacgatcg aagcaatgat
     2401 tacaggaata tgcatggttg ttgcattgat tggaccgata gggataaaac ctaaaggcgt
     2461 aacagctaat aaaatcaaaa tagctaaaaa catcgcagtt aatgtaaagt tttttgtatt
     2521 tttcataagt actccttttt tctaagcatt tctttcatta agagtcagca tgacctgttc
     2581 gatgatcgtc tgaatatcag ctaaggcacc ttttccaaaa tctccacaag ctaaaagaga
     2641 ttctcttggt tcaatttcct gatagccgac agatctcaat gtttccagat tacgctgagt
     2701 gatcgggttt tgatacatat tcgtgttcat ggcaggggca atcaattttg gtgttcccac
     2761 tggaagcgcc attgcgactg ttgaaagaag atcgtctgca atgccgtttg ctagcttgcc
     2821 gataatgttt gctgtagcag gcgcgatgag aaacaagtca gcttgttttg ctagttcgat
     2881 atgattgatt acctgcggat ttgattcttg catgacgtct gtatggactg cgcgcttcga
     2941 cagagattgc aaggttaatg gcgtgataaa atgtgtgctg cttttagaca taatcacatc
     3001 tacctgggca cctaatttga cgaattgact tgtaagatca gcgcttttgt aagcagaaat
     3061 acttccagta acgcctagta agatcttttt cattttgatt cctccagtct aattttctca
     3121 gtaatcaatg cagcaatctc agatttactt tgagcttctt ccactgttcc atctttagat
     3181 aagagatagc cgtggtgtgt ctcgccatgg atttccgtca aatcatttgc gaaaataaaa
     3241 tcagttttgt tatttatcag actatttttt gcgactgcca gaagagattc ctttggtaca
     3301 tctactaata gtttgaaacc aatgattttt gcttttggct gccattcacg tagataagaa
     3361 atgatttttg gtgttttctt caaaacgagc acaagatgct cggtgttaga agagatcttt
     3421 gaaacagtgg tatctgtctg ttcattttcc gcaaaccaat tgtccagttc ttcttgcgtt
     3481 gaagaagtgg gtaggttttt tgccagctgt tcttctgaaa aactaaaagc aggagtaaaa
     3541 tcactgactg ccatactgtg gataatcgca tcgtagtgtt cttttttcgt tagttcttct
     3601 aattgtaaaa ataagtccag tgcagattcg atcgtataaa tcgttatatt ggagtgtctt
     3661 ttcggtttca gtgcttgttt cgtagtcacg taatcgattg tggtatccgg attggcagct
     3721 aaataatcag ctaatgcttg ccctaatcgt cctgtcgaat gatttgtaat agcacgtact
     3781 tgatcgattt tttcagtcgt tcctccagct gtgattagaa tcttcattta tggtcaccta
     3841 catttactag tgttcttcct tggtgtgttc cagcaagtag tttagatgct gtttcaggca
     3901 attgatccag agtaatctct tggacgacca gttcatctgc aatctgcaaa tccgccatct
     3961 gttgccataa agaaagacgt tgatctattg gtacattgac agaatcaatt ccaatcaact
     4021 ggatgtttcg cagaataaaa ggcaagacag tcgcgtttag tttgattccc ccagcattgc
     4081 cacataaagc tactgcaccg ttataagaga tcaatggaag aagactgctt agctgctcgc
     4141 cacctactgt gtcgatgaca tagtcgattt gctgttttcc taaagctttt gttgtctctg
     4201 gcagaaattc atcaggtcgt acgacttgca aagcacctag actttttagc catgtgacag
     4261 actcttcttt tctggaaaag gcaatgattg actggaatcc tagtttatgt aacagggcta
     4321 tcgctgtact gcctacaccg ccagaagcac cagtaacgac aactttttta tcggaggtca
     4381 taccttgacg gattaaagcg ttcacacaca acatagcagt aaaccctgct gtaccaagga
     4441 tcatcgcttg acgcagcgtc atattttttg gtagaggaac taaccagtct cctggtactt
     4501 tttgatattg gctataaccg cctggatggg tgacgccaag cccaaaacca gtagcaagta
     4561 caagatcttc ttttgaaaaa cgtggattgt tactttggac gacagttccg gataaatcaa
     4621 tccctggagt catcggatat gttcgtatca cctgaccaga ctcgctcatt gccaatgcat
     4681 ctttataatt gacatcagaa taggcaattt ttaccaagac ttcattttca gccaactcag
     4741 taaatggcag atcactgatt gctgtatgga aaacaggttc ctttattacc tgaaaagatc
     4801 gaaaagcgtt cattcgtgag acactccttt taaaattttc ttttatcata ccataaagcc
     4861 tactctttta ttcttgttct tccgtaaatt tagacaaaaa ttaggtgaaa aataaagaat
     4921 caaacaacta gaatttattc caaaagataa agaaatattc agcaaaaaaa attttacaaa
     4981 gggttgcctt tttcaataaa tttcgtatac taatacgagt ggtagtaaac aggaagttta
     5041 ctattagaaa acttcgattc ggaggaaatt atggaaattg gtgagaagtt aagaaatttg
     5101 cgcatccaaa agaatttgac acaagaagaa ctaggcgaac gcacagattt gtcaaaaggg
     5161 tatatttccc agttggaacg agatttaagt tcaccatcaa tggaaacatt cttttctatt
     5221 cttgaagtct taggtgtgac tccagaagaa ttttttcatc aagaagcagc gaatctgcaa
     5281 gtggtttatt caaaagaaga tcatacggtt tatttcgatg aagaaaacgg gtatgaattg
     5341 gaatggctcg tggctgattc gaatgaaaaa gaaatggagc ctgtcttgct gacttttgaa
     5401 ggtgcaggag aatataagac gtttgaacct tcattatctg aaacgtttat ttttgtatta
     5461 gaaggggaat tagagttaag cttaggagag acggtctatg tggcaaaagc aggacagtcg
     5521 atctattatc aagcgacaaa acaacatcaa ttaaggaatc atctcaaaaa acgaacaaaa
     5581 gtattgattg ttgcaacgca atcatatttg taatataggg ggtaaaaacg tggggaaacc
     5641 aatcatttca tttgatcaag tagtgaaacg ctatgatgat gaaacagtat taaaaaaagt
     5701 aagttttgag atcgaacaag gaaaatttta tacactgctt ggtccttccg gctgtggtaa
     5761 aacaacgatc ctacgtatta tagctggatt caccgaagct acagaagggg acatctattt
     5821 tgaagggaaa cggttgaatg atttgccagc taataagcga caagtaaata cagtcttcca
     5881 agattatgca ctttttcctc atatgaatgt ttttgataac gttgcttttg gattgaagat
     5941 caagaaaatg tccaaatcag atatcgaaaa gaaggtaaaa gatgctttgc gtatggtcca
     6001 gcttcctgga tacgaaaaac gggagattag tgaaatgtct ggtggtcaaa gacagcgtgt
     6061 agcgattgca cgagcaattg taaatgaacc gaaagtattg ctattagatg aaccgttatc
     6121 ggcgcttgat ttgaaactac gtacagatat gcagtatgaa ctgcgggatc tgcaacaacg
     6181 attaggaatc acgtttattt ttgttactca tgaccaagaa gaagcgttgg caatgagtga
     6241 tgagattttt gtcatgaata aagggaaaat cgtccaaagt ggtacacccg tagatattta
     6301 tgatgagcca atcaatcatt tcgttgctga ttttgttggg gagagcaata tcgtggacgg
     6361 tgtaatgatc gaagataatc tagttgagtt tgttggtaag caatttgaat gtgtcgatgg
     6421 cgggatgcgt ctaaatgaac cagttgaagt tgttttacgc ccagaggatt tgacgatcac
     6481 gacaccagat aaaggaaaac tagtcgtaac ggtcgatact cagctgtttc gtggtgtcca
     6541 ttatgaaatc atctgttatg atgaacagca aaatgaatgg atggtccatt caacgaaaaa
     6601 agctaaagaa ggaagcaaag taggattggc ctttgagcca gaagatatcc atgtcatgcg
     6661 tttcaacgag tctgaagaag aattcgatgc acgtcttgac agctacgaag aatagggggg
     6721 acaagaatga aaacaatgcg caggatctat tccgtccctt atattttgtg gctcggttta
     6781 ttcgtgatcg cgcctgtatt aatgatcatt tatcaatctt tttttgatat gaatgggcaa
     6841 ttcacgctga ataattatct atcttatttt acgtcgggga catcgctttc tatgacgatc
     6901 aattctgtat ggtatgcatt tttgatcacg tttttcacgc tgatcatcag ttacccgact
     6961 gcttattttt taacaaaact gaagcacaag caattatggc tgatgctgat tattttgcct
     7021 acatgggtga atctgctgct taaagcttac gcatttattg ggattttcag tgtgcacggc
     7081 agcgtgaatc atttctttga ttttatcggg atcgggccga aacagatatt attcacagac
     7141 ttcagttttc tgttcgtggc aacgtatatc gagattccat ttatgattat gccaattttc
     7201 aatgcattgg aagagatgaa tccttctttg atcagtgcta gtagagattt aggcgcgaat
     7261 agctttgaga ccttccgacg agtgattttt ccgttatcat taaatggtgt gaaaagtggt
     7321 gtccaagcag tatttattcc ttctctttct ttattcatgc tgactcgtct gattggcgga
     7381 aatcgggtga tcactttagg aacggctatc gaacagcatt tcttggttac acaaaactgg
     7441 gggatgggtt ctacaatcgg tgtgatttta atcattgcta tgttcatcgt gatgctcctg
     7501 acaggcgaga agaaaaaagg aggtcgctca taaatgaaaa aatttaaatg gtcgtatctt
     7561 tatttaggga tcgtcttttt attcttatat gcaccgatct tctatctgat tttttattcc
     7621 ttcaatgaag gaggaacgat gaacagcttt acaggattta cttgggaaaa ttatgaagct
     7681 gtttttgaag atacacgttt gatcattatc gtattgaata cgttcatgct ggctttctta
     7741 tctgcattga tcgccacgat catcgggaca tttggggcta tgggaatcta ttacacgaaa
     7801 aaacgacgtg cacggacagc gttgttgagc atgaataaca tcttattagt ctctcctgat
     7861 gttattatcg gtgctagttt cttgattttc tttacactac taggattcgg attaggtttt
     7921 cagagtgtat tgctttccca tattgctttc agcattccaa tcgtcgtctt gatggtcttg
     7981 ccaaaattgc aggaaatgaa cgattcaatg gtcgatgcgg ctagagacct tggtgctaat
     8041 aatttccaag tagtcaaaaa tatcattttg ccttatcttt ctccaggaat catagctgga
     8101 tattttatgg catttaccta ttctcttgat gattttgcgg ttacattttt cgtcacagga
     8161 aatggcttta cgacgctctc agtagagatc tattctcgtg caagacaagg aatcagttta
     8221 gaaatcaatg cgctaagtgc gttagttttc ttgttctcca tgatattggt cattggatac
     8281 tactttatta gtaaagaaaa ccgtccaaaa cggctgaaga aaaacagaag agtacgcgag
     8341 ggggtggcac ggctgcgatg aaaaaattac aatctctgtt tttaggtatt ctcatcatta
     8401 ttgcggtgct gctgcttggt gttcgtcaac ttgaaaaaac aagtggcatg agtggagcaa
     8461 aggtacttac catctataac tggggagatt atatcgatcc tagtttgatc acgaaatttg
     8521 aaaaagaata tggatacaaa gtgaattatg aaacatttga ttctaatgaa gcgatgttca
     8581 caaaaatcca gcaaggtggg acaaactacg acatcgctat accaagtgaa tatatgatcc
     8641 aaaaaatgat caaagaaaaa ctagtacttc cattagatca ttcgaagatc aaaggattaa
     8701 ataatattga tccacgattt ttagacttgg attttgatcc agataatcag tattcgattc
     8761 catatttttg gggaacttta gggatcgtat ataacgataa attctttgat gcaaatcaaa
     8821 ttaagcattg ggatgatttg tggaagccgg aattaaaaga cagtttgatg ctgattgacg
     8881 gtgcacgaga agttatgggg ctttctttga atagtttagg ctattctttg aatagtaaga
     8941 atatggcaca gctgacagaa gcagcaaata aattgaatca tttaacacca aatgtcaaag
     9001 caatcgtggc agatgagatc aaaatgtata tgatcaatga agaagcctcc gtggcagtga
     9061 cattttctgg cgaagctgcc gatatgatgt gggaaaatga acatttacat tatgtgatcc
     9121 caccagaagg atccaatctt tggtttgata atatcgttat gccaaaaacc gtgaaaaata
     9181 aagaaggtgc ctatgatttc atcaacttta tgctagaacc ggaaaatgcg gcacaaaatg
     9241 cagagtatat tggttattct acaccaaatg aagaagcaaa agcgttattg ccaaaagaca
     9301 tttcgtcgga cgaacaattc tatccatctg atgacacgat ctcccatctt gaagtctaca
     9361 aagatctagg atcgaaatac ctaggcatct ataatgacct attcttagaa ttcaagatgt
     9421 ataggagata atggcaaaac tcagtaagaa tggggactta tcaacgttct aaagagctaa
     9481 aaatcgtcct ttttctatct gagttttcca atcttctacc aaaagcggat gaaacgccgc
     9541 tttttttgtg ttattttatt ttttctacca cgtttttcta ccagagaaaa gtggacaaaa
     9601 ccattgtaaa atagggcccg agacgggcta gtaaatctaa gattgaaaaa gttacagaag
     9661 gataaagtaa ttggaaacta tgcaaatata cttaaccc
//
LOCUS       DAXWOO010000107         9607 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.108, whole genome shotgun sequence.
ACCESSION   DAXWOO010000107 DAXWOO010000000
VERSION     DAXWOO010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9607
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.108"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            735..1538
                     /locus_tag="ACGE70_002013"
     CDS             735..1538
                     /locus_tag="ACGE70_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFF8693639.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKDLSDKLGNLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
     gene            2123..2578
                     /locus_tag="ACGE70_002014"
     CDS             2123..2578
                     /locus_tag="ACGE70_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693640.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            2697..3755
                     /locus_tag="ACGE70_002015"
     CDS             2697..3755
                     /locus_tag="ACGE70_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HFF8693641.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLDERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVSKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            3797..5746
                     /locus_tag="ACGE70_002016"
     CDS             3797..5746
                     /locus_tag="ACGE70_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFF8693642.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKSEDKENEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQL
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFK
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            5749..9390
                     /locus_tag="ACGE70_002017"
     CDS             5749..9390
                     /locus_tag="ACGE70_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3659 domain-containing protein"
                     /protein_id="HFF8693643.1"
                     /translation="MKFKKYLLQNLRIKRWVVRGLVIGGVLAVTLGAPFGMNDPTPDN
                     QDSKQETVINKNVAYAESKPAEQGMWTLYARILMGDKEVNDSAAKTGQMNLLGQGGIS
                     ADFPYSDISKQGNSIAGGNAGTQLASTLATYSYYGYIETVSGNAIASQASSILGSIGR
                     TIGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGEGGSDVSNPVSKALTSLL
                     NNLGLNNELFKAIASLSMLFFTAILGYQGMKLLSKDGVNSKGLSPVLEKWIVRIFVLF
                     IFLPVAGSLLGVYINGIQSAKKDGDIPTNVVSDYIINTRLWASTQNLAPSSDTGGDVP
                     DGKPTSEIKFVDTDYEPSKKSAMIRAINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NINTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGSAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTAHNFDATQPWGVANNATFSTQSVALLLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFIRIWEVVLTGSLASGAGVMAISLGVVTTSLIGLQLPIFLITFIRAFGDTAV
                     GFIPNTLVGDGVANIVRSAMILGVGYLLVFKKGEKVNNLINMLIGLPLEVAFGFDERA
                     KLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGNSSVKDMVAGGLGAVGAQKMAER
                     LNNMGDSNADAKSIADKVIPETPEGMSFNGGGVGSTINNTSFKDLVRQDQLRNNSQQY
                     IDHLGRMVDENGNFVDENGEILKDSKYGKDAKGNFIDEKGRLVDEDGNLIDTNGKPLE
                     DSEDGNIGMDSEGRLIDEKGRLVDEDGNLVDENGRRLLNGDFGHDSNGRLVDEKGRLV
                     DEDGNLVDEFGNKLGKHSDGYNVNGDLVDEDGNIIETRDALVADRNRELEQQRHTPIA
                     VAIPENEEDEITLDQTHQELRNEMSGEDVSDRVHRELGDGASGESQSRELNSNFDNSA
                     IRNDTVLKDRESLTKETDKEQSDVKKKFIPTPNVNQTDKYETNIGSGMSKDNQSIREP
                     ELTNKNNRTNNSKSTSDNSGKSNQSTKKTSTAKSAVEYLSKTGKNVYTSADKALAKAP
                     KGSLAGITYEAKNEAIKIGKGTAKVAKATATGASKTGKVVKKSTMSVKDYIVKYGNQK
                     STENKSEDE"
BASE COUNT         3441 a         1384 c         1964 g         2818 t
ORIGIN      
        1 ttttcttttt ttggatatat tcggaatacc acatcgcttt ttgtccacgt ttgcatcgct
       61 ttttgtccac attcacatcg ctttttgtcc atgttcgcat cgctttatgt ccaccctaaa
      121 atctatttaa gattgatata aagccttttt ggaggttgct aaagttttaa agattaaaat
      181 atatattata aatataaata aaaggggaag ctgaagcttc ctccttttcc tttttttcaa
      241 ttattcaaag ttacacaagc aaaataaact cctagatagt gttagttttt tttaagattt
      301 tttattttta agattttgaa ataagagagg agtaatcctc ctttattaaa tatctgttaa
      361 tatatctgtt aaatatctgt taatatatct gttaggggag gcttaatctc ttgggagagt
      421 aagtattaaa ggggtataaa ggtaacactt ttggacagta aaggtaacaa aagtggacag
      481 taagggtaac agttttggac agcaaaggta acaaaagtgg acatttgagg tatcacacta
      541 ggataagacg ttaccttcgg ttttcttgat ttggaaggcg aaaaaataga aaattagatt
      601 agtaacaaat ctttgttgac tgttaccttt agttgtgatt taatttaggt aacaagcaga
      661 aaaaattgtt tcctttaatt agtaccagat taacatgatt aaaggagcgc aaaaagaaga
      721 ttggagagta atcaatgtct caagaagtta ccgaaaagat tattgatagt gatgtagatt
      781 tagtatcaga acaaacaaat gcaattgtgc gttataacaa tggtctgaat acaacagctt
      841 tgcgaggatt taagcctgtt gaaatggatg tgttttgggc tattttatca aaaatgaaac
      901 gtcaaggaac agcagaagta acttttgatt tcaacctctt tcgttatttg gtcaaatacg
      961 acagaagaga aaaggacagt ttttattctg tgttaaaaga tttgagtgac aaactaggta
     1021 atttaaccta ccgttttgaa gattcagacg tctatgaaca gctgtggctt tttcaaaaat
     1081 tccggataga taaacagaag gaaacagtta caattaaggc aaatgaggaa tttgaattta
     1141 tcttaaactc aattggcaaa aactttactc gttttgaact tgataatttt acaaaactaa
     1201 gaagtagcta cacaaaagaa ttgtatcgtc atctcatgca atacagaaat aaagagtggc
     1261 gaaatggtta ttgggtagtc aaagttgaag attttagaag agcattaagt ataccttcaa
     1321 gttatagaat gacaaatatt gataaaagaa tactagagca ggcgaaagaa gaatttttag
     1381 ctcctgatga aaacggaata caggtatttg aaacttttga ttatgaaaaa atctatgcaa
     1441 agaaaggaag aagagttgat cggctcagat tcacttttag tgagccagaa tcaaacttgc
     1501 caaccatttc aatgcataat tggttagaag aagattgaga agaagcaggt tgtaatttta
     1561 atacaacctg cttctttaaa aaagtatggg taacactttt ggacaataaa tgcaagaagt
     1621 ttttttgtaa gcaagtttca gtctaaataa tatttgaaag aatggctaat ctatacaaat
     1681 tttaacaact atgggtaaca cttttggaca agtcaagaaa aaaaacaatt tttcttaatt
     1741 tgttgatcga ctaaaggtaa cacttttgga caacacagaa taaaaagtcc ggtgatatca
     1801 gttttggtag gaaacatctt aatatctttg tgagattgaa ggtaacattt ttggacaagt
     1861 caactatttg ttaaaagtta atatagagta attagataag ttttttcttt tcgtctgata
     1921 aatggataat attagttatt ttattgctaa ttagtctttc atgatgactg aaatagctaa
     1981 aataaatatc tcgttggata attgctgata ctttaaggga tatctaaatt tgctgattga
     2041 tttatttatg ttattatgat attataataa cataaatgtg ttacgcccga acctcatttt
     2101 aaaaaaatga aggggtgaga caatgaatgc tattaattgg catgaatttg ttaactatat
     2161 ggagagctta ctagatgatc taaaaagttt tagtattgct gatgcagtaa agcatgggtt
     2221 accagttagt acaacgatga aattacgagg attgaaaatt ccagatgaaa ttattaagct
     2281 gagaactaaa acaattattg aaaccgcaaa agtcgttgac aatatgaacc atgaactaaa
     2341 aggcaagaaa acaacttacg agaaagattt tgaacgtttt ttaaaatctg ttattggaaa
     2401 atatctaggt aaaaaatctt tcagtgaatt gagagatgaa gagattgtac ctactccaat
     2461 ggttagcaaa atccgaaaaa acaaagccga ggggaattac tatcttggta caatagcaaa
     2521 aatcattgat tatttggaaa ataaagagca aaagaaagga acgaagaaag gaagataatt
     2581 tttgtgttaa atattaatgg ggaagaatat accgaaatga ttcaaaatcc cgaacctaga
     2641 attaccgaaa aaacgtctga ttgtttgctc ttttatgaaa gggggcagac aaactaatgg
     2701 aaaaaaacgt caattcaaca tctaccaaag atcaatacag tattattgga gggttgcttg
     2761 gtgataagga taagagaaca cgtcacggat tacgcattat gcttgcaact tttctaggag
     2821 tgcttttttt attaacaggc ttgggtagct acatttatca attaggaaac tataattatt
     2881 atctcgtgaa caatacaaca cctataggct cttcattgga gtttgctcga tcaggagcag
     2941 agatcactat ttcagatgtg tggacagatc aaaacagaga tgtaacagta gtaaaactcg
     3001 gttactcgca aaacgctaga aagttgttat ctacgcaagg aaaaaactac aacttatata
     3061 tggcaacgaa agaaaaagat cgtcctaaaa agcttgaaat gagctatggg atgttaagta
     3121 ctgatgggga tgctttctta ttcctaaaag gtaaattaga tgaaagagct tatcaagttt
     3181 ttattgcaaa ccaagtatca ctaatttctg gtgatgatac agctgacgat gataccaaag
     3241 tcaatgtgaa tgaagcagat tcagttacaa aagcactttc tcaatattct atgaatgatg
     3301 ttaataatga aggagtaatt tcttttggga aaaaagcaaa acctgttaat actcctgata
     3361 atatcaattt cagaataaat ccatattcag attcaactaa tgtctatcaa ggaagctttc
     3421 ttaccaaaga tggcgatatt gattattcaa aagttgtagc agttacaagt aagaataatg
     3481 ttgtaagtaa gttagaagat cagttaaatg cttcaaatac taacatcaag aaaattgaag
     3541 ctacaaaagc tgagtatctg gaacgtttac aacaaaatcc tgacgataaa gattcgcaag
     3601 aaagtattcg tcaactagac agtcgaatgg agaccgaaat taaagcaatc aatgatacac
     3661 aaaaattgat tgatcaatac aaaaatgctg actttaacaa agattctttt ggagacatga
     3721 agacgacatt taagtaccta actctgactg actagaagta aattattctt attttagagg
     3781 aagtgaacac aaacagatgg aaaatagtac cgagtttttt aagaatattc aaactgaatt
     3841 taatcatgct ctcacaccaa tgaaaccacc gaaacataag ttagtgggac gtgatgacga
     3901 actccatgag ttgtcactga tcttgaatag accggaaaca ccgcaagcta tcttgttagg
     3961 tgaagccgga acaggtaaaa cagcacttgc aagtgaatgg gctgaaagac agcgacaaga
     4021 tggaaagaaa gtacaagtat acaaattatc aatcggacaa ttgcgtgttg ggggagatga
     4081 tgtcctccaa acacgtttag aaacattaat tccaagattg aaagaatatg agcgaatttt
     4141 aaaatctgag gacaaagaaa atgaatttgt cctcttcatt gacgagtttc ataaagttgt
     4201 tagctctttt ggtagtggca ataaaattgg gggtgattcg ctaaaagaac cgttagcaaa
     4261 tgaatcactt caattgatta ccgctactac aagaaaagaa tatgaaaact atatcaaaag
     4321 agatgatgcc ttagggagac ggttacaacc gttagaattg aatgaactaa aaagcgatgt
     4381 tgtcgtcgat attttgagac aatggctaaa tacatcctct gttaaagatg atcctcaggc
     4441 ttcttttaaa ttatcgcaaa ggattcctga tgaattactt caagaaatcg tacaagcaaa
     4501 caagctttat cgtgaggatt tgtccgaacc ggctaaatca attgccgtaa tggaggattt
     4561 gattgcaaaa gatcgagtag acggaaatgc ttataatcga gaaagtctat tgcaaatatt
     4621 tggtaagtat aatattcagc tagattctga ttttgatatg gaaaaacttc aagaggaatt
     4681 aaagtccata aaagggcaac cgttagcagt ttatactata acaatgttgt ttaaacgtat
     4741 gaagcttgga tttgatcgtt ttaaaggtcg aaagcaacca aaagcaactg ctctgtttac
     4801 aggatcaaca ggggtcggta aaactgcaat ggctaaagcc gtggcaagag gactttatgg
     4861 cgatgaaaat aaagttctct tagttaatat gccggactac aaaacaaaag atgatgcaat
     4921 gcgttttaga cgactcatag gtactcatgt aaggcatgat ccttctgcga ttattgtgtg
     4981 tgatgaatat gagaaagctg gtgtagaaac actcgatgcc tttttatcaa ttttagatga
     5041 aggtaggata agttttactg aaagaggatg ggatgatcga gaagttcacg acactgtttc
     5101 attgtcaaat acaattttat ttgcgacaac aaatgcaggt gcggagatgt tcgattcgtt
     5161 aaacagatat agctcactat ctcgtgttac aaacaaggaa aattctaatg aatatagcac
     5221 tgaactaaaa agagaatacc gaaaagtcgc atcagggatc aaacaagcct tgattggtga
     5281 aggtctaaag cctgaagtta ttaaccgatt tggacgaata gttccatttt gggctttatc
     5341 cgaacaaact cttctggaaa ttgcttataa tgaaatgttg aagagtgctg aattagtacg
     5401 aaaacattta aatatagatt tacgtcttcc acctaggaaa gaaagagtat atggtcaaaa
     5461 tttttatgca gacgatctct ccatgcactt agtttttgac atggcttcac aagaggattc
     5521 taaatcaggg ggtgcaagga atatcttgca tttagttgaa tctgagtttg aagaagcgat
     5581 tactgaagca ataattactt atccaaacgc aaaaatattc gatgttaagt tgagtgaagc
     5641 cggagcgttt gaaggaaaag gtgattctaa tgctgaaatt attgcaaagc cttccgttgc
     5701 tgaaagtaaa gaaaacaaga ttgtattttc gattggaggg gcgtagctat gaaattcaag
     5761 aaatatttac tacaaaacct tagaataaaa cgatgggtcg taagaggtct agttattgga
     5821 ggagtattag cagttacttt aggcgctcct tttggaatga atgatccaac tcctgacaat
     5881 caagatagca aacaagaaac agttataaat aagaacgttg catatgcgga aagtaaaccg
     5941 gcagaacaag gaatgtggac actatatgct cgtatcctaa tgggagataa agaagttaat
     6001 gatagtgccg ctaaaactgg acaaatgaat ttgttaggac aaggtggtat cagtgccgat
     6061 ttcccttatt ctgacatttc taaacaaggt aattcgattg cagggggtaa tgcaggtact
     6121 caactagcat ctactttagc aacttatagt tactacggat atattgaaac agtctcagga
     6181 aatgctattg ccagtcaagc atcatccatt ctaggaagta ttggtaggac gattggtagt
     6241 tttatcggat caattagttt aatcctttct aatattgctg aatccataaa tgaactcctt
     6301 ggaaaatttt tcatgagtat gaatatattt tctcttatgg ggtggggaga aggtggatcg
     6361 gatgtatcta atccggtatc taaagcatta actagcttgc ttaacaattt gggattaaat
     6421 aatgaattat ttaaagcgat agcaagctta tctatgctct ttttcacagc aatactaggt
     6481 tatcaaggaa tgaaattatt gtcgaaagat ggtgttaact cgaagggatt atcaccagta
     6541 cttgaaaaat ggattgttcg tatttttgta ttgtttatat tcttaccagt ggcaggatcg
     6601 ctattgggag tttatatcaa cggcatccag tcagcaaaaa aagatggaga tataccaaca
     6661 aatgttgttt ctgactacat cattaacaca agactttggg cttctactca aaatcttgct
     6721 ccatcatctg atactggtgg tgatgtacct gacggaaagc ctacatccga aatcaagttc
     6781 gttgatacag attatgaacc atctaaaaaa tctgcaatga taagagcgat caatactagc
     6841 agttatgcca gacaaaataa tgatctagat tctaattcag tcggtatggc tctattaaaa
     6901 gaatggtcat cgaatgatac tttcaatatt aatacttatg ccggtgatgt taatgtcgcc
     6961 gaaaatgatg cttatatggg aagagcgcct gctgtttgga caggctataa tttaggggat
     7021 ggaaaagaag gaagtgcaat taagaaacca accaatttaa atggatatat ttggtcagca
     7081 aatcaaaatg tgacagacga caatcgcaag cctgatggaa aaggaacagc gcataacttt
     7141 gatgctactc aaccttgggg cgttgctaac aatgcaactt ttagcacaca gtcggtagct
     7201 ttattgctcc aatcggcact tgatacatcg ggagctaagt tttatgcgta taatatcgct
     7261 ccttcaggaa ttcaaggcag tgctaagaat atgtcaacag taaaaacaga atggcgtaca
     7321 gcatccttag ttggtcatgg agctatgggt aagtttggct cgtggctagc gatgaacgct
     7381 gaatcaatcg ttttagcagt tctttactta gcatgtgctt gggcgatgtt caatgtgaat
     7441 atcgtgaaag catttaaacg tgaatttatt cggatttggg aagtagttct aacaggatca
     7501 cttgcatcag gagcaggtgt gatggcaatt tcattaggtg ttgttacaac atctctgatc
     7561 ggactacaat tacctatctt tctgattact tttattcgtg catttggtga tactgctgtg
     7621 ggattcattc ctaatacatt agttggcgat ggcgtagcaa atattgttcg atctgctatg
     7681 attttaggtg taggatatct tttagtcttt aaaaaaggcg aaaaagtaaa taatttgatt
     7741 aatatgctta ttggcttacc acttgaagta gcgtttgggt ttgatgaacg tgctaagtta
     7801 ttatttggta atagaaatga tgctgtgcga gttgtggacg gcgtttctaa agcaatcaag
     7861 aaaagccaag gagctaaaaa tccctattat gatggtggca acagctcagt aaaagatatg
     7921 gttgcaggag gtcttggagc agttggagcg caaaaaatgg ctgaacgttt gaacaatatg
     7981 ggagatagca atgctgatgc taagtccatt gctgataaag taattcctga aacaccagaa
     8041 ggaatgtcat tcaacggtgg cggagttggt tccacaatca ataatacctc tttcaaagat
     8101 ttagttcgtc aagatcaact acgtaataac tcccaacaat atattgatca tttaggacgt
     8161 atggttgatg aaaacggaaa ctttgtcgat gaaaatggtg aaatattaaa agatagtaaa
     8221 tacggtaaag atgctaaagg caactttatt gacgaaaaag ggcgtttggt cgatgaagat
     8281 ggaaacctta tagatactaa tggtaaacca ttggaagata gtgaagatgg taatattggt
     8341 atggatagcg aaggacgttt aattgatgaa aaaggtcgct tagtcgatga agatggaaat
     8401 cttgttgacg aaaatggacg caggttgcta aatggtgatt ttggtcatga tagtaacgga
     8461 cgtttagttg acgaaaaagg tcgtttagtt gatgaagatg gaaatcttgt tgatgagttt
     8521 ggtaataaac ttggtaaaca cagcgatgga tataatgtga atggagattt agtcgatgag
     8581 gatgggaata tcattgagac gagagatgcg cttgtggcag atcgaaatag agaactcgaa
     8641 caacaacggc atacgccaat tgctgttgca attcctgaga atgaagaaga cgagattact
     8701 ttagatcaaa ctcatcaaga actgaggaat gaaatgagtg gtgaggacgt gtcagacaga
     8761 gtgcatcggg aattaggaga tggagcatct ggtgaaagtc agtcaagaga gcttaacagc
     8821 aactttgata atagtgctat tcggaatgac acagtactta aagacagaga atcgctaaca
     8881 aaagaaacgg ataaagaaca atcagatgtg aagaaaaagt tcattcctac tcctaatgtt
     8941 aatcaaacag ataaatatga aacaaatatt ggatcaggaa tgtcgaaaga caatcaatca
     9001 attagagaac ctgaactgac aaataaaaat aatagaacaa ataattctaa atctacttca
     9061 gataattcag ggaaatctaa tcaatcaacc aagaaaacat caacagctaa atcagccgtt
     9121 gaatatctat caaaaactgg taaaaatgtt tacacctcag cagataaagc tttagcaaaa
     9181 gctccaaaag gtagtctagc agggataacc tatgaagcta aaaacgaagc tattaaaata
     9241 ggtaaaggaa cggctaaggt tgccaaggct actgctacag gagcatccaa aactggtaaa
     9301 gtagtgaaga aatctaccat gtcagtcaag gactatattg taaaatacgg taatcaaaaa
     9361 tcaactgaaa ataagtctga agacgagtaa aaagaaggag cattgatttg ctccttcttt
     9421 ttactttgat ttctaactga agatgtcaag gaaaatttct atttatgcaa aaaaattatt
     9481 ttaaattgtc tataaatgaa ttaaatattt actgttcaaa gttgtataat taaaaagggc
     9541 ttatgctggt aaagaacaga attattatct aaacaaaaac ttgataaacc agcattttta
     9601 gccaata
//
LOCUS       DAXWOO010000108         9527 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.109, whole genome shotgun sequence.
ACCESSION   DAXWOO010000108 DAXWOO010000000
VERSION     DAXWOO010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.109"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(<1..608)
                     /locus_tag="ACGE70_002018"
     CDS             complement(<1..608)
                     /locus_tag="ACGE70_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HFF8693644.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSK
                     RILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRK"
     gene            852..1499
                     /locus_tag="ACGE70_002019"
     CDS             852..1499
                     /locus_tag="ACGE70_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693645.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(1592..3871)
                     /gene="helD"
                     /locus_tag="ACGE70_002020"
     CDS             complement(1592..3871)
                     /gene="helD"
                     /locus_tag="ACGE70_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFF8693646.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKNLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            4149..5972
                     /locus_tag="ACGE70_002021"
     CDS             4149..5972
                     /locus_tag="ACGE70_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFF8693647.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            6019..6198
                     /locus_tag="ACGE70_002022"
     CDS             6019..6198
                     /locus_tag="ACGE70_002022"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFF8693648.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            6535..8475
                     /gene="thrS"
                     /locus_tag="ACGE70_002023"
     CDS             6535..8475
                     /gene="thrS"
                     /locus_tag="ACGE70_002023"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFF8693649.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            8699..>9527
                     /locus_tag="ACGE70_002024"
     CDS             8699..>9527
                     /locus_tag="ACGE70_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFF8693650.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     "
BASE COUNT         2988 a         1713 c         2004 g         2822 t
ORIGIN      
        1 aatttacgcg taggcaagac atcaattaat tgtcctgttt cgccatctgc gcaaataaag
       61 ctcattttat cttctatgga agcgtgcgaa cgaaattcat caaccatcaa tactctgggg
      121 agaatcctct tagattgctt tggtaagtag cttttaaact ctttcaacgt acgaataacg
      181 gtggtcaaag atacctgaca atttttcgca ataaaagata aagatacttt ttcagtcagt
      241 aaagaagcaa ttttatatct gacatgattc gcgattgaat gtctgggttg aacaaaataa
      301 ctttgagccg tccaatgggt tcgacagttt ttacaggtat agcgctgctt ttttaagcgc
      361 ataaccaaag gcatgtgatt gtattgttca aagcggacaa tcgtttcctt cttcccattt
      421 ttcactacaa ttactttccc gtttccatct accacagtag aaccacaatt tctacaggta
      481 tggggagcag gtgagaaaac agcatcgacg atcaacgtct ttttcttctg aaaggtctcg
      541 taagagacct ctgtaatcat caaatctttc tctattaatc gcagcatttt tttgatagaa
      601 tcattcatat agcatatcgt cctctcagtt gtttattttg tcgtgattta atcatactag
      661 agaacgatat gttttttaat acctaaaata aaaatggggc tgaagaatca attctgattc
      721 atcagtccca taaattatag agcccttttt tgtgtgattt attttctttt ctaattcaac
      781 ctgtggtatg atgagagcat aagtatcatg tgaaatgata tgaagtatgt ggaagatgga
      841 cggggaagaa tatggaagca aaaatcactt tagaaccttt tgaacgtatc ttaagcggtt
      901 accgcaaagt agaagagtta gctgtgaacg taactgattg ttcaaaatta gcgcaaaaat
      961 acgcaagatt cggcgttgaa ggctatcgat taggaaacta tgtcggtaca gggtatctga
     1021 accgttattt agagtgtatg gtggaccgcg caccaatgct gatttataga caaaattatc
     1081 tgattccgct gctttttcgt cgttctgatt cagcctttcg gttatttgaa gaagagtatc
     1141 ggatggaggc tttctttcta ctattagagt ggagcttgaa acatcgtcct gaaaaaattc
     1201 ttatcgaaag aaatgagaag atagacacga aaaaaaataa agtgatcgat agtgcatacc
     1261 tagcatttcg tgtatcagag attttggatt gtggcggcta tccaatcagc aatttccaat
     1321 caattgacca attcattgaa tggaatcgaa tttaccgatt gatcgacaat ggcgggattg
     1381 gccgtcatag caaggtattt gacccagaat atccagaaaa catggaagaa ctgaaaatga
     1441 ttatttcgct tgtcaaatta aaatatcctg aaacagattt ggatttatat atagaataaa
     1501 tgaccttaaa tgattgaatt actttttctg caattattat tgtaaataaa aagacaactg
     1561 ctaacattaa tgatagcagt tgtcttttta cttacagtac ttttttctca taagatgaaa
     1621 tatccatatt gtttaacaaa ggactttcat tattggaggt aaaaagggtc aattcatgca
     1681 tcgcacgtgt acacatggtg taaaggatca atttgtcttg ctgagaagaa aaatgttctg
     1741 catctacatc ccacaagaaa actcggtcaa actccagacc ttttgctaaa aaggctggaa
     1801 gtaaaataat ggaacgcttc ataaagtcac tctcatttaa aatcagctgg acttgttgtt
     1861 tcaactcgtc tggcagttga gcataaagag cttcgcattc ttcttgtgtt ttagctatga
     1921 ttgctgtacg ccaataccgg gcttttccct gagtcgttcg aatggtatct gctaaccaat
     1981 tcaatttgat atcatctgtt gttccttgga tgagcaaagg aaggtctcct tttcttgctg
     2041 ttgtttctac ttgatgttct ttcacaataa aatgattagc aaaatcagtg atttcttttg
     2101 tggagcgata gcttgtcgtt aattggaaac gagtgacttc acggtccacg aaaaggtctt
     2161 ccaatgatcc aacgatcgtt tcatttccaa agacattttg atttagatct ccacagaaag
     2221 tcaaatttgc ttttgggaac aaggcttgca gcaatgccac ttgtgatggt gcgaaatcct
     2281 gcatttcatc aataaagata tagcgagctt tgtgtgtaac cgtcactgga tatgttcggc
     2341 gcatcagtaa aaagaatagt acagcatcct cttgacgcaa ttctctttga cgcaagtgtg
     2401 tacgaacagt tgtcaaatgt tcctgccacc tggtctcgct gattttatgt cgtgttagaa
     2461 gagaaagtgg cacagtttgt agaaagtgga gatattgttt cgttatattg acaaattgat
     2521 aattcatgat ccctcgatgg attcgacgga atcttttacg tacgatttgg ttggccaggt
     2581 ttttacgtaa tgaacgctcc ttttgttctg tatattcaag atttggatct gttgcgtata
     2641 gttcttggag ctgttcttcc gccagatctt tgacccattg ctgacgtgtt tcgtcttttt
     2701 gcagaccgcc taattttttc aggagcttcg tttgtaatag agaaagtcta tgatgtaaag
     2761 gtaattgttc atttgtttct ttataccatt ggcgaatcga ttcctttgat aggatcgtac
     2821 ggccatttat tttcatatca cggaacaagg gaccgtagtt tgtgatgcct tggatatagc
     2881 gatcaatctg atcgactaag gtcaatccgc ttttcatcac ttgtatcgga tctttttccc
     2941 ctagcaaaaa accactttct tgttgctgtt catctagtaa agaaaactct ggcagcagct
     3001 gcaccaaata atttttgaat gtctgtgtag gcacgccgct ttcacctaat gaaggcaata
     3061 cagtcgagat gtaatcagaa aacaaatggt tgggtgaaaa tagaagaacg ttttcagcct
     3121 cgagccactt gcgattatga tacaacagaa aagcaatacg ttgaagcaaa gcggatgtct
     3181 ttccacttcc ggcgattcct tcaatcagca ttactttact tgttgtgtcc cgaatgattg
     3241 cattttgtgc tttttgtatc gtagagacga tgtttttcat atggttgctt gatgattcat
     3301 ccaatatctc tagcaaaaag tcatcattga ttacttccga cgtatctacc atagataaaa
     3361 tacgtccttc ttggatttta aattgtcgtt tcaactgcag atcgacatct attctatctg
     3421 tttcagtttc ataaaatgcg ggaccaatct caccttcata ataaagattg gcaattggtg
     3481 cgcgccagtc aataacgatt gtttcttctg ccagatctct taaagaagcg atacctagat
     3541 ataatgtttc tgtttcacgg tcttctttaa agtcgatacg ggcaaaataa ggactatctt
     3601 tcatgacaga caacgtttgc aggcgttttt gctgtgcttc aagagtatgg tatttcaaaa
     3661 gcaattcctg ctcatgttgg cgatattcaa cagcagattc ataaaaagct tcattggaac
     3721 cagctcgaat cttgtgatta gctgtgtctt ttgtcgaatg attcatttcc tgagtcaatt
     3781 cttgttgttt tgtatccagc atattttgtt cgatctggat tagttcgatc gtttcatcca
     3841 cgtgcgtttg ttctagttgt ttctcattca tcgcatgttt gctccttccg ccgtaaataa
     3901 cgaattaaaa gtatagcata gtttttcgaa aaaaggtact ttttaactga tgaatcgaaa
     3961 aagtaatcct tgctgaatat ttttgtttgt agaactgatg aattttggaa acgctgatag
     4021 tgatagttgt tttcaattaa ggcctattgt tttaaaaagt gctagtcaag gactatgctt
     4081 aagatgaaag aggcagctgg aaatttatta ctaacctctc taagtcacat aaaagaggag
     4141 aaaaaacaat gacacatttc aaaaaatttt tacttacttt gcttattggt gggatagctt
     4201 tattaagtga atttgttttt caccagccgg cccttgcatt tgcgattgtc gcatttactg
     4261 gagggattct cgccttcttg atgtttttgg aaatgatcaa gacgttacgt tcaggaagat
     4321 atggcgtaga tgtattagcg attaccgcaa tccttgcaac attagccgtc aaagaatact
     4381 gggctagttt gatgatattg atcatgctta cgggcggaga tagtctagag gattacgcga
     4441 ataaacgagc gggtaaagaa ctgcagtctt tgttagataa caccccaaga attgcccatc
     4501 gattcaaaga aggaaaattg caggatatag taatcgatga aataaatatt gatgaccagt
     4561 tactagtcaa gcctggcgaa cttgtacccg cagatggtcg agtgaccaaa ggagaatctt
     4621 cggtcgatga atcttctttg acaggtgagt cccgcccgga aaccaagaaa cctggagatc
     4681 agattatgtc agggtcttta aatggagacg gatcgatcca aatgatcgct gaaaaacgtg
     4741 ccgcagacag ccagtatcaa atgattatta aactagtcga agaatcaaaa gaaaagcctg
     4801 cccgatttgt ccgtttagct gatcgttatg cagtcccttt cacgttgatt gcttatttga
     4861 tcggtggtat ttcttggtgg ttctcaaaag accctttacg atttgctgag gtactggtag
     4921 tggcatcacc ttgtccactg attctagctg cgcccattgc tttagttgca ggaatgagcc
     4981 gttcaagtaa aaacgggatc gttgtaaaaa ctggaacaac gattgaaaag ctcgcacgta
     5041 caaaaacgat tgcttttgat aaaacaggaa cgcttaccaa agggatattg gaagtagagg
     5101 cgattcagcc agtcaatgga ataaaaaaag aagagctact tctgtatgca gctagtgcag
     5161 aacaggaatc tagccatatc ttagctcgtt cgttagtgaa caacgtgaaa aacagccaac
     5221 tgtatcctgt tagtcaactg aaagaagtga ccggtcaagg aatccaagca gtagtcaata
     5281 atcatgaagt aaagattgga cgtgcctctt ttgtttcagc tgaagaaata gaaacgtcca
     5341 atacagcaat ctatgtaaca attgatcgat cttttgcagg aacgatccta ttttcggatc
     5401 agattcgccc agaagcagca aagacaatca aggagctaag aaaacttgat gtgaaaaatt
     5461 tgatgatgat caccggggat ggatctgcca ttgctcaaac gattgcagaa aaagtccaat
     5521 tagattctgt acatgcccgt agcttgcctc aagataaact gaccatttta gaaagtatcc
     5581 ctaaagatca aagacctgtt gcgatggtag gagacggcgt gaatgacgca ccagcacttg
     5641 ctgctgcaga tatcggtatt gcaatggggg ctcatggttc tacagctgct agtgaaagtg
     5701 cagatgcggt catcttgaaa gatgatctct tgcgtgtagc aaaagcagta gtgatttcaa
     5761 gagatacgat gagaatagcg aaacagtctg tactgattgg catctttatt tgtatttttt
     5821 tgatgttaat tgcaagtacg ggactaatcc ccgcattatt cggagctgtt ttgcaagaag
     5881 tcgtggatac ggtctctatt ttgagtgcct tgagagcaaa aaaagatcca gccatttttc
     5941 ctgattcagt acccaagaca aacatagtat gatagaatcg agttgaaaaa agaacgatca
     6001 gaaagaggag agagcattat gccattcgta catgtcgaat tagtagaagg tcgcagtccg
     6061 gaacaattag aaaatatgat gaaagatatc acggaagctg ttcataaaaa cacgcaggca
     6121 ccaaaagaac acatccatgt catcatcaat gagatgaaaa aaggaacata cggtgtaaat
     6181 ggggaatgga aaaaataagt ttagtttccc acttgatttt tctatttatt agtggttaaa
     6241 taaacacata attgattgat gagaaagcca actgaatttg agagtcttcg ataagagagg
     6301 aaaagctagg ctgaaacttt tccaggagaa aaccaaagga tgaccacttt cgaaaccctt
     6361 gttgaagcaa gggcggtcgg accgttacaa cgcttgagga acgcatttgc gtttgaacat
     6421 taggtggaac cacgaacttt tcgtcctagt atctcttttc aagagatgct aggatttttt
     6481 tgtttttcag cttgatgatc tgatcaatca aaacatttca aggaggaaac tattatgata
     6541 aaaattactt ttccagatgg tgcagtcaaa gaatttgagt ctggaacaac aacattggca
     6601 attgctgaaa gcatttcaaa aagcttagca aaaaaagctt tagctggtaa agtgaacgga
     6661 aaactcgttg atttgactcg tccaatcgag gaagatgcaa gtattgagat catcacacca
     6721 gaccatgaag atgcacttgg cttagtccgt cattcagcag ctcatttgat ggcccaagcc
     6781 atgcgtcgcc tctatccaaa tatccacttt ggcgttggac cagctatcga ttcagggttt
     6841 tactacgata cagataatgg acaaaaccaa gtaactgcag aagatttgcc ggcaatcgaa
     6901 gcagaaatga tgaaaatcgt caaagaaaat ctgccgatcg aacgccgtgt tctttcaaaa
     6961 caggaagcat tagaaatttt tgcaagtgat ccgtacaaag tagaactgat tagtgaattg
     7021 ccagaagaag aagtgattac tgcttatcaa caaggcgaat tcatcgattt gtgtcgtgga
     7081 ccacatgttc catccactgg ccgtatacaa gtatttaaat tattatcagt ggcaggtgct
     7141 tattggcgtg gaaactcaaa caatcaaatg atgcaacgtg tatacgggac agcctttttt
     7201 gacaagaaag ctttgaaaga atatatccgt ctccgtgaag aagcaaaaga aagagaccat
     7261 cgtaaactag gaaaagaact tgacttgttc atggtttccc cagaagtagg ttctggtttg
     7321 ccattctggc ttccaaaagg ggcaacaatc cgacgcacga tcgaacgtta tatcgtagac
     7381 aaagaagtta gtctaggtta ccaacatgta tatacaccaa taatgggaga tgttgagttg
     7441 tacaaaacat ctggacactg ggatcattat caagaagaca tgttcccgcc aatggatatg
     7501 ggagatggcg aaatgcttgt tcttcgccca atgaactgcc ctcatcacat gatggtctac
     7561 aaaaacacga tccattctta tcgtgaatta ccaattcgta tcgctgaatt aggaatgatg
     7621 catcgctatg aaaaatcagg tgctttgtca ggtttacaac gtgtacgaga aatgacgttg
     7681 aatgatggtc atacatttgt tcgtcctgat caaatcaaag atgaatttaa acgtacgctt
     7741 gaattgatgg tggcagtata tgctgacttc aatattacag attatcgttt ccgcttgagc
     7801 tatcgcgatc caaacaatac agataaatat tttgatgatg acgcaatgtg ggaaaaagcc
     7861 caagcaatgc tgaaagccgc tatggatgaa ttagaactag attactttga agcagaaggc
     7921 gaggcagcct tttacggacc aaaactagat gttcaagtca aaacagctct tggtacagaa
     7981 gaaacattat caactataca attagacttc ttattgccag agcgctttga tttgacatat
     8041 gttggagaag acggggaaaa tacgcatcgt ccagtagtta tccaccgcgg aattgtttct
     8101 acaatggaac gttttgtcgc ttatttaaca gaagtataca aaggcgcttt tccaacatgg
     8161 ttggcaccta tacaagcaac aatcatccct gtttctgttg atgcacatgg cgattatgct
     8221 tatgaaatca aagaacgttt gcaaatgaag gggttgcgtg tagaagttga tgatcgtaat
     8281 gaaaaaatgg gctataagat acgtgcctct caaacacaaa agatccctta ccaattagtt
     8341 gttggagaca aggaattgga agatgcaaca gtgaacgtac gtcgttatgg aagtaaagaa
     8401 acagctgtag aagatttgaa tatctttatt gatgctatgg aagcagaagt caaaaattac
     8461 agcagagaaa actaataggt aaatttcctg taaaaaagta gtagatagaa agcaatccga
     8521 acaatagata acaggtattc ctcaaagaca cagatgctca atagaatctg tgggattgat
     8581 gggataagat attctgttgg gcggattgtt tttatgtttt ttatccagta tacgaccgtg
     8641 aataagggtc tgatatggta tacttacata gactatcatt aaaagggtgg aggaacttat
     8701 gagcgtaatt agtcgtattg aaagtatgat ggaagattat tcttcagcag aaaaaaagtt
     8761 ggccaactac attatcggta atattgagaa ggtccctaca atgacagcta atgaattagc
     8821 agaagcttcc gggttgagtg cgccgacagt tgtacgtttt tccaaaaaaa tcggttttca
     8881 aagtctgacg gacttcaaaa tcacgatttc aacagaacta cagactggaa tcgatgaagg
     8941 ttttagtgat atagaagcaa atgagtcctt ttactctatc aaaaacaaac ttggtaacaa
     9001 tgcacaagta gcgataaaag aaacagtaga tattctagaa gaagaaacga tccaaaaagt
     9061 cgtagagtgt ttggaatcgg cagagactgt ttttttatat ggggttggcg cctcttcttt
     9121 agtagtagaa gatattctgc aaaaatggtc tcgtgtcggt aagccaatca tttttgaaaa
     9181 agatatccac gtcttgctcc cgcaacttgt cagcaatgag aaaaagaaag tgctttggct
     9241 tgtttcaaac tcaggtagaa gtgcagatgt agtcgcatta gccgagctgg cgaaaagcat
     9301 gaatatcgaa atcattgcgt tgacgcaatt tggaaataac ccgcttagca agatagccga
     9361 tgtactcgta caaacatcta gaccaaaaga aatcacgaat cgaagtgcgg caactaattc
     9421 acttttggcc caatttgcta cgatcgatat cattttttat ttctatatgg ctaaaaacga
     9481 aaagcttagc gaaaaagtga aaaaaacacg agaagcaatc caagaat
//
LOCUS       DAXWOO010000109         9409 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.110, whole genome shotgun sequence.
ACCESSION   DAXWOO010000109 DAXWOO010000000
VERSION     DAXWOO010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.110"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            239..2074
                     /gene="tdc"
                     /locus_tag="ACGE70_002025"
     CDS             239..2074
                     /gene="tdc"
                     /locus_tag="ACGE70_002025"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFF8693651.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2379..3857
                     /locus_tag="ACGE70_002026"
     CDS             2379..3857
                     /locus_tag="ACGE70_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFF8693652.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3823..5154
                     /locus_tag="ACGE70_002027"
     CDS             3823..5154
                     /locus_tag="ACGE70_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFF8693653.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5268..7820
                     /locus_tag="ACGE70_002028"
     CDS             5268..7820
                     /locus_tag="ACGE70_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFF8693654.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7827..9266)
                     /locus_tag="ACGE70_002029"
     CDS             complement(7827..9266)
                     /locus_tag="ACGE70_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8693655.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
BASE COUNT         2944 a         1567 c         2009 g         2889 t
ORIGIN      
        1 gtttgttcgc tttttttagg ttatctattt tagaagactg atttattgaa aaattgttcg
       61 cttttttgcg ttgaattatc agaaaaatcc gactttattt atttttgttt gtaaaaatct
      121 acgtggattt gatgttataa aaaaaaaatt ctcatttttg gcgctatagt gaagaggtgt
      181 tacagataaa cacatgtatc tgcaagacat tgaccaacaa aatatggagg gaagaaaaat
      241 gaaagatatg gatatcaagg ccgtctttat aggcgacaaa gctgaaaatg gaccagttta
      301 taaaatgttg ttgaacaaga tggtagatga acatcttgga tggagagaaa attatattcc
      361 ttcagatatg cctgcaatta gcgaaggaga taagctcact ccagattatc tagccacccg
      421 cgaccatatg atagaggtct tggatgaagt cagtcagcga ttacgcgcag gatcgatccc
      481 atggcattca gcaggccgtt actggggaca aatgaatgct gaaacattga tgcctgcttt
      541 attagcttac aactatgcga tgctgtggaa cccaaataat gttgcattgg aatcttctat
      601 ggcaacttcg caaatggaag cagaagtagg acaggatttt gcttcattat tcaatatgac
      661 agacggatgg ggacacattg cagcagatgg ttctattgct aatcttgaag gattatggta
      721 cgcccgttgc atcaaatcga ttcctttagc tgtcaaggaa gtgctgcctg aaaaagtgaa
      781 aaaaatgtct gagtgggaac ttttgaattt atctgttgaa gaaattttgg aaatgaccga
      841 aagctttacg gacgaagaaa tggatgaggt caaagcagct tcttcccgta gcggaaaaaa
      901 tatccaaaga ttagggaaat ggctggtccc tcaaacaaaa cactattctt ggatgaaggc
      961 gttagatatt tgcggagtcg gcttagatca gatggttgct atcccagttc aagaggatta
     1021 tcgaatggat attaatgctt tggaaaaaac aattcgtgaa ttagctggac aaaaaatccc
     1081 aattctaggt gtggttgctg tcgtaggaac gacagaagaa ggtcaagtag acagtgtaga
     1141 taaaatcgtc caattaagag aaaggttaaa agatgaaggg atctatttct atttacatgt
     1201 tgatgccgca tatggcggct atgcgcgttc actgtttcta aacgaagcag gagaatttgt
     1261 gccgtatgct tccttagctg aattctttga agaacatcat gttttccacc actgcgtaac
     1321 gattgacaaa gaggtatatg aagggttcag agctatttca gaagcggatt ctgtcacgat
     1381 agatcctcat aagatgggct atgtccctta tgctgctgga ggaatcgtaa tcaaacataa
     1441 aaatatgaga aatatcattt cttatttcgc gccatatgtg tttgaaaaat cagtaaaggc
     1501 tccagatatg ttaggagctt atattttgga aggatcaaaa gctggagcaa ccgccgccgc
     1561 agtctggaca gcacatcgtg tattgccatt gaatgtgacc ggatatggtc aattgattgg
     1621 ggcttcaatc gaggcagctc aaagattcag agaatttctc gatcatctga cttttactgt
     1681 taaaggaaaa acaatcgaag tctatccatt gaatcatcct gattttaata tggtcaattg
     1741 ggtattcaaa gaacaaggct gtacagattt gaacgctatc aatgaattaa atgaaaaaat
     1801 gttcgatcgg tcttcttata tggatggcga tgtttatggt gaacggttta ttacttctca
     1861 tactacattt acacaagaag attacggtga ttctccgatt cgttttgttg aaagaatggg
     1921 tctaacgaaa gaagaatgga aaaaagaaca gaaaatcact ctgctacgtg cggctatcat
     1981 gactccttat ttgaatgatg atcgaatttt caatttttat acaaaaaaaa tcgccaaagc
     2041 aatggaaaag aaactaaacg aaatcatcca atagaactga tttttgtgtg aaagaataac
     2101 aagagacata tggagaacag acaattattt gcttcatcaa aacaattgta tttccccctg
     2161 tttttcatat attcctcgcc aagatattct tttttataaa acgttctata agttggatcg
     2221 ttcgtttcat gtatcaaacc cagttcacta agatcgttca atggatgttt cggtgatact
     2281 gggttttgta ctgaatcgat gaaaccttgt catttctgtt cttgaacaaa aataaaagaa
     2341 aacaaaaaca tatgtcgcta ttgaaagaag gaataaagat gaaagatact ggttcatcaa
     2401 agcaaatcac gaccatgacc tttatcggaa tgacctgcgc ccttgtagca agtgtacgaa
     2461 atattccaga tgtagcagca acgagctgga cgatgatttt ttacatgctg gttgcagtat
     2521 tattgtacgc ttttccaatc tcattgatct ctggtgaatt tgctggaatg tttccccaaa
     2581 aaggtggtcc agaactttgg gtatcgaatg ctctaggaaa aaaatggggg ttcgttgtct
     2641 catggctgtt atgggtccaa atgtttcctg ggatggtgat ggtcgcttca gcactcgctc
     2701 cgttgtttgg taatattatc gataatgtgc cgcttggttt gaacagcaaa tttacattag
     2761 ttgtcatttt agtcgtttat tggatcatca cttttttgaa tttgaaattt gatatggcaa
     2821 aaatcggcgg aaaagtcggt gtttggttag gattgtacgt gcctttgacg atgatgctct
     2881 tattagggtt tgctgcgtgg atcaaagttg ggatcgtacc aaccggaaca ttaggcagtt
     2941 tttcatggga caaattaata ccagatacta cgactgcatc ctcatttgtt tattttgcgc
     3001 cgatcatgtt tatttttact ggtatcgaaa tgtcctctgt gtatatcact cgattggaaa
     3061 atccagttaa aacgtatatt cgcggagtat ttgccgcgtt aatttttata tttttcgtca
     3121 atattttgaa tgctttagtt gtagcaaatg tcgtaccaaa aggacaaatg gaattgaata
     3181 atatcgcgca atctatttcg atttattgcc aaattcttgg tttgccacat cttattgtga
     3241 atctgtttag tttacttgtc ttcattggtg tagcagtcca attatctgct tgggcctcag
     3301 gaccagcaaa gacagtgaca gaaagtgcta gaaaaggtgc gtatccacca aaattcaatt
     3361 tttggaagac gaatcaattt gacgtatcca aatccgttat tttgactcag tctgtcatta
     3421 tctcaatctt tgcacttttt tatttgctga ttcctggcgt caatcaagca tttttgatgc
     3481 tcgtaaattc aactactgtt atctactgta tcgtctatgt catcatgggg atcgcagttt
     3541 tgagattgcg ttatacacat gcaaagttga atcgaccatt tagaattgga aaaaaagaga
     3601 aaaagagcaa tcttggagtt tggactgtcg ttattgtttt atttgcggca atcggatttt
     3661 ctgtcggact gacgatgaaa gctggaacat ggatcaatct aattgcagtc acagccatca
     3721 gtgtcatttt gtttgtagta ccactctgta ttgaaaaaat aaagaaacct tcttgggaac
     3781 aggaagtcgc catgctgctt gaaaaagaaa aaggtgaact tgatggaaaa accggcaatg
     3841 aaacggactc taactaatcg aaatatccag ttaatcgcat taggtggcgc aattggaaca
     3901 ggattatttt taggttcagc aaaggcaatt gcatcagcag ggccagctgt tattctcgtt
     3961 tatttgatat gcggtttggc tatttatggt ttgatgcggg ccatgggtga tttattttta
     4021 gcaaatagta catttaactc tatcagtgat ttcgttgact attatttagg caaaaagtgg
     4081 gcattttttg tcaattggac ttattggctt tgctggcttg gagctggact tgcggaatta
     4141 acagcaatag gattgtatat ccatttctgg tttgtacatg taccagcttt gataagcgga
     4201 ggtagcgcac taatgatctt aatggcagtc aaccttgttg cagtcaagtg gtttggtgaa
     4261 ttggaatctt ttttctcaat catcaagatc ttggggattt tcctttttat cttattgggt
     4321 ataggattga gtgtctcaga ttttcatacc cagtctatcg gaatcaaaga aaattttgga
     4381 cagatcgatg aattactacc gtttggcttt gctggaatgg tagtctcatt tcctatggtt
     4441 ttattcagtt ttgctggaat tgaaatgatc ggcttaaccg ttggcgagac aagtgatccg
     4501 caaaagaatt tgaaaaaagc aatcaactgt ctgccttggc gtattctctt tttctatata
     4561 ggaacgattt tgtcgatttt atttgttgtt ccatggcatt gtttagattt aaaagaaagt
     4621 cctttagtga ctatgctgca agtgatccat tgccaaggcg gagcagcaat tgtcaatcta
     4681 atgattttgg ttgcttcttt gtcctcagca aatagtgcag tctacagtac tagcaggatt
     4741 ttatatcaaa cagcaaaaga aaaaagtttg ccccattggt tttgtgcgct ttctaagaaa
     4801 ggcgtacctt tacacggtat tttaattaca ggaatgttat tttttgccgg tctttgtcta
     4861 catttttttg tagctgatag tcgattgttt tttcagcttt tggcaggcat gacaacgagt
     4921 tgttttctat ttgtgtggtc ttcgatcttg tgtgcacatg ttcaatttat taaagaaaag
     4981 aaacggaaca ctacacaaag atataaagat cttgctttgt tggtgttttt tacttcaatt
     5041 gctttcagct tgttattcga aagcacgaca cgctttattc cttttttatt ggttatctgg
     5101 tttgtattct taaattttgt atataaaaga ataagcaata gaaatatcag ttaggcccag
     5161 atacattttg caaaacgaat tatttcagac ccccctgaaa taatcgatac gcctgaaaaa
     5221 acttccctgc gtataataac agtatcaatt aaacaggagg ggataggatg aactggtaca
     5281 gattgacggt agaagatgta ttgaaaaact atcaaacatc aacagaaggc ttgtcgcaag
     5341 aagaacgaca gaaacgcctg actgatcaag gatataatga aataaaagca aggcagcaga
     5401 caaaacgatg gaaaaaaata gcaaagcatt ttactgatct gctgatgatc gtgttgatca
     5461 ttgcctcttt tttgaaattt gcttctagtg aatatattga aggaagtatt attttattcg
     5521 tagtgatcgt caatggactc gttagttact ggcaagaaag aaaagcggaa gaatcattga
     5581 atggtttgaa gcagctgatg ggacaagaag cgatagtact atcaaacggg atgcaagaaa
     5641 aaatccctgc gaaagaactg gtaaaaggag atgtcattgt cttacgtgcc ggagatgtcg
     5701 ttccagcaga tatccgatta atcgaagcac atgatttatt agtggaagaa tcgattttga
     5761 cgggagaatc agaagcatct gaaaaaagtc atgccgtttt gacagaagaa gaatcgatcg
     5821 gcgaccagaa aaatatgggt ttttcaggaa cgcttgtaca atctggttct gcagtcggtg
     5881 tcgttgtaga aacaggaaac acgacagaaa tcggtaaaat caatcaagca ttgcaatcgg
     5941 tcgaacaaca aacgacgcct ctgattaaaa aaatcaaccg attaaacaaa caaatttttc
     6001 aaggcattct ttgtttgagc ctattcttag ttatttttac gacatttcga tatggtatgg
     6061 attggcatat tcttctttcg gctacgattg ctttggtggt gtcaatggta cccgaaggat
     6121 tgccagctgt tttgaccatg attctatccg ttggagtaca tgaaatggca aaagaaaagg
     6181 cgatcatcaa aggacttcct tcggtagaaa cattaggttc tatgacagtg atctgttcag
     6241 ataagacagg aacattgaca aaaaatgaaa tgacagttgt ggatgtagct gcaaaagaag
     6301 aagcctgcgt gttgtccatt atgaaaaact gtcaggaatt aaaaacaaaa gaagaacaga
     6361 aaacagaaaa tttaagtggc aatccgacag aagttgcgct gattcgacac acagaaacag
     6421 ccaatctacc tttgaaaaaa atagaagcaa agattccgtt cagttctgaa tacaaatata
     6481 tggcaacgat gcatgcggag gaaaaaggag cggtgattta cgtcaaaggt gcaccagaag
     6541 tattatttgc taaatcaact ctttcttctg cagaacaaga agcatggagt cagacagctg
     6601 cagaatttgc gagaaaaggt cagcgtgttt taggatttgc ttataaaaaa gtagattcga
     6661 aacaggaact cacgcatgag actttaaccc aattaacttt tgcagggatc gccggattga
     6721 tcgatccgcc aaaagaaagt gcagtcaaag ctgtaaagga atgccaacaa gcagggatct
     6781 cagtgaaaat gattacaggg gatcacaaag acacagcaaa agcgattgcg gaacaaattg
     6841 gcttaaaaca tactgccaaa gttttagaag ggatcgattt ggatcttatg tctgatgaag
     6901 agttgattca gcaagtgcca atagtcgatg tctttgctcg aacgacacca gaacataaac
     6961 tgcgaattgt taaagctttg cagaaaaatg gagaaatcgt cggaatgacc ggagacggcg
     7021 taaatgatgc acctgctttg aaaagatccg atgtaggaat tgctatggga atcaaaggta
     7081 gtgaagtaag caaacaggca gctgatatgg ttttaggaga tgataatttc catacgattg
     7141 caaaagccgt aaaagagggc cggcggattt tggataattt gcaaaaaacc atcaatttct
     7201 tcttgccaac tgcattagct caaggattga tcttgatttg ggcacttatg ctgaatcgcc
     7261 cgctaccttt gtccccagta cagattttat gggtcaatat ggtaacgaca atcactttat
     7321 cttatgcact agggttcgag tcgccagatc cagaaatcat gaagcgccca ccaagagatc
     7381 caaagcaagg aatcctatct ggttatcatt tgtttcggat catctatgtc tccctactga
     7441 tcatggttcc tgcctattta ttggctatgc agtttgaagg gcaaagctta cagcaaacca
     7501 ttcttctgca aaatatcgtc ttagctcaag ctgtttacat gatcaattgc agagagttgt
     7561 ccaagccgtc attgaaccaa ggtcttttgc aaaacaaagc gctttttctt tcattaggta
     7621 tccttgcttt attgcaaggg atggttattt ttctaccagt cggccagcag ctgatcggta
     7681 cagtaagttt aacgatgaca caacagttat tgattggagc aaatgtgatt cttctattct
     7741 tagttgtaga aatagaaaaa gggatcatga acaaactgtt tcgcaaaaaa gaaaaaacag
     7801 ctagagaata cagtcgctag ccgttactat aaaaaagttt tgaaagtgag ttcagtcaaa
     7861 cgattgagct catttttcgt tggaatagaa gaaatgtata caatagggat atctagagaa
     7921 tctagaggga aattggaaat caagatatct gttttttcaa aagaaatagt ggaaagatcg
     7981 gttagttcga taggtaagaa gcagacacgt tttcctaagt aatcttttaa ttcctgctgc
     8041 aagaacgcct tccatgaagg ttctccttgg aaaataaaat agacattgaa caaattcggt
     8101 ttgatagaaa aaatcgtttg ttggatcagt agagacaaga gttggagaaa gtaatttggt
     8161 tcattgattt ctatttcttt ttttagatcg ttcagtaaat tctctttctg ttgatacaag
     8221 tgtgcatatt gctgatcata tgtttctaaa aaacgatggt attgacggtc caatgcttca
     8281 atcaatatcg gcgattcatg atattttaat atgaggagag ccaagttatc agtcagagat
     8341 gtttttttta gttgatcaag agacagctct gaagctagtt cctctacaaa cgtttcgagg
     8401 acctctaaaa atggagaatg atgcaggcag cgagcggttt cttgtccata gttattgttg
     8461 taattccaac tttccagaaa gcagaaagaa gcaaaagaaa gctcagtgtc tttaagataa
     8521 ataccttccg ttaaatacaa ttttttcaat gcagagtcat acagggagga gagtgtgctg
     8581 ttggtatgga tagaagaatc taatcggcaa ttcgctttta tacggatcgt attgataaag
     8641 aaccaacatg ttccgaaaat ctcttctgtt tcaaccgcta gttcagtttg actgaggttt
     8701 cttaaaaatt gaaaataatg ggattcatgg atcgaataat cctcgaaaaa atagttgtta
     8761 tgagtgaaag gaatcagcaa tcgatgataa aaagtacgaa tcgcacgctc ttttccaaga
     8821 atacaagctg tatatttcgt caaatcgatc tgcaagtcgt aacttgctaa acgctgattg
     8881 attttagtca catgtctttt caatgtccca taagaaagcc catggtcggc tagaaaatca
     8941 gggacagcaa ctttttttgt aaaaagcaga tttttcagca gttcgacact gactgattgt
     9001 ttgaaaaaaa tctcccaaag gtcatttgtt aataaatttt gctggttttg caaactgatt
     9061 cctgcttgat cgctgatgat gtcccagcct tcgggcagct gagagcgaat agattgaagg
     9121 tcagaaaaaa tcgttcgttc ggttgtccct atgatttcag ctaattcatt tacggttatc
     9181 tttgtatgat tcagcaattg ttctactaaa gaaacatgac gtcgaatatc tttttcggta
     9241 atgatttttt tcattagtga atacatcaat cagactccct tctattctaa gaatacggag
     9301 aaaataaacg gtggtcaagt ttcaactgat ttttcaatca ttgtttattt ttctggagca
     9361 tttactgatg ataagaattt catcatgaac ttgaaaaatg ataccacta
//
LOCUS       DAXWOO010000110         9311 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.111, whole genome shotgun sequence.
ACCESSION   DAXWOO010000110 DAXWOO010000000
VERSION     DAXWOO010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.111"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..435
                     /locus_tag="ACGE70_002030"
     CDS             <1..435
                     /locus_tag="ACGE70_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFF8693656.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(759..842)
                     /locus_tag="ACGE70_002031"
     tRNA            complement(759..842)
                     /locus_tag="ACGE70_002031"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(806..808),aa:Leu,seq:cag)
     gene            1370..1870
                     /locus_tag="ACGE70_002032"
     CDS             1370..1870
                     /locus_tag="ACGE70_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8693657.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(2060..2404)
                     /locus_tag="ACGE70_002033"
     CDS             complement(2060..2404)
                     /locus_tag="ACGE70_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFF8693658.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(2558..3478)
                     /locus_tag="ACGE70_002034"
     CDS             complement(2558..3478)
                     /locus_tag="ACGE70_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFF8693659.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            3665..4156
                     /locus_tag="ACGE70_002035"
     CDS             3665..4156
                     /locus_tag="ACGE70_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFF8693660.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(4180..6555)
                     /locus_tag="ACGE70_002036"
     CDS             complement(4180..6555)
                     /locus_tag="ACGE70_002036"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFF8693661.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            6881..7507
                     /locus_tag="ACGE70_002037"
     CDS             6881..7507
                     /locus_tag="ACGE70_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFF8693662.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            7530..8234
                     /locus_tag="ACGE70_002038"
     CDS             7530..8234
                     /locus_tag="ACGE70_002038"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFF8693663.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            8426..9259
                     /locus_tag="ACGE70_002039"
     CDS             8426..9259
                     /locus_tag="ACGE70_002039"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFF8693664.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
BASE COUNT         2834 a         1793 c         1656 g         3028 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacctg ttcaagctga acaacctgtt
      121 gtacaagaaa cggttcaaac ggaaacacaa gcagcgcctg ttgctgaaac acaacctgct
      181 cctgctgtaa cagaaactgc tgctactcca gcaagtacaa gctctgcaaa agaatggatc
      241 gcacaaaaag aatcaagcgg ttcttacaca gcaacgaacg gacgttacat cggccgttac
      301 caattagatt cttcttactt aaatggtgac tactctgctg caaaccaaga aagagtagca
      361 gaacaatatg ttacttcacg ttatggttct tgggaagcgg cgaaagcatt ctgggaagca
      421 aacggttggt actaagattc attatttgaa cgtttaaaca cattcaaaat atagtaagag
      481 gtcatgcgat ttattctttc gcatgacctc tttttgagtt taagtttagt ctttattcct
      541 taactatcgt ttgccagtat ttcttgtatc agaatctata aatctactat ttgaatcaat
      601 ctattaggtt ttaaactgtc ccaccttcac acaaattttt tttgatttat ttgaatctgg
      661 ttccgttgtt taaaattttt cctatattct tttagataat atgttatttt ctttctcatc
      721 gctccaggtg taccttctct ttataaaaaa acaaattatg cggctaagaa gactcgaact
      781 tccacgaggt caccctcact agcgcctgaa gctagcgcgt ctgccaattc cgccatagcc
      841 gcatcacatc ttttagttta ggattacatg tgttttttgt caagaacata tttttgttct
      901 ttttcatttc tgtacgccct cccttatgcc atctacacaa ataattcatc cgagcaactc
      961 catcatctat tgagggttgg tgaaaaaatc tgttttatcc atatgatcga tttcaatgtt
     1021 aaacgaacga ctctgggatt agtaactatt ttattttttt cttcatttct tttttcctct
     1081 ttttctcgat tcagctatct tctttcattt ggatattgat tatttcttct gttgattact
     1141 atccttctgg aattctagac gctgaagtaa ttcacgtttt ctttcttcta gagatcataa
     1201 gtaacgaatg aacgaacttc aaataatttt tttattgtaa ttcatatttg tattgttcga
     1261 tgattcttaa tacgatatga cacaataaat aaaaataggc atggctattt acaacaatag
     1321 tgctatagta gaaaacaaat gaataaggtt acttaaagag gtgaatacga tgaaactgat
     1381 caaactatgg gaagcagatt taaagaaagc ttatcagcta caaactagct ttggaccaaa
     1441 tgagagtgga tttatcaaca atgcttatgg ctatacattt gctgaatttt tagattatgt
     1501 aaaaaaatgc gaaaaaacgt ctttaggaat cgatcttcct gagagttacg ttcccgcaac
     1561 cgtctttatt ttagaaagtg atgaacatga gtatgtagga atttttaatt tgcgccataa
     1621 actgaacgaa acattaagaa acggcgctgg ccatattggt tacggcatct cccctaatta
     1681 tcgtaaaaaa ggatatgcca cacaaggatt agcattagta ctacaagagg caaaaaaaat
     1741 cggcattgat gaggcacttt tgtctgtaaa taaaaaaaat cttggcagtc ttaaagcaca
     1801 acaaaaaaat ggggctgcga tttatcgtga agatgagaaa gaatactata cgaaaatcaa
     1861 actgatgtaa aaaagaagga ttccaaaagg aaagggattt gaatgtcctt tatcaataga
     1921 acacatttat ttaattaact tataatatag cagacaaaat tttataaaca aagaaacctt
     1981 ttatctcttt tcttctagaa aggataaaag gcttttttgt ttttcctatt tgcttctcat
     2041 agctagtcag ccaagatttc tatcgtatcc ccaacgcaat ccgtgcataa cggctcatct
     2101 tatctgtcgt ccatgctgga taccagacta gtttgacttc tgctttcgtt acttctggca
     2161 cttcttttaa tgcgtcatgg atagattctg ttaaaatatc tgctaatgga cagcccatag
     2221 ttgttagagt catcttaatg actgtttctc cattttcagg attgaattct atatcataaa
     2281 tcaagcctaa attgacgatg tcgattccta attcaggatc aatcaccatt tccaaggcag
     2341 caagaatacg ttctttgatt agttctattt cttgtccaga ccgttcttgg tttgcttcgc
     2401 tcatcgtacg acactcctta acacgatttt tatttatcat actctttttt tagctgcttg
     2461 taaatgagaa tttagaagta agattctcgt ttaaatttat aaaaatgttc ttattagatg
     2521 taatagatta aatttattct aaaataaacc tgattcctca ttttttttgt gaacgatttt
     2581 ttatttctgg caatacgcca gttgttcgat acattttttg ttcgttttca tttgcaaacc
     2641 ggccaaatac tgaatgagtt ggtaaaatat tgataaatgc atcgtcgtcg atttcatgaa
     2701 cgatctgatc aagatcatac atttcgtagc gggtgatgac catcatgatc atgttccctt
     2761 gtttttttga atagccaccg acagacggca gtaatgtcat tccacgtacc atccgttttg
     2821 ctagttcttc tgttacctct tctggcttga cggtaacgat cattgctgtt actttttgat
     2881 gactcgtgtg gatggcatca attacttgag tcattgcata aatggaaata atcgtataca
     2941 aggcactttc ccagttaaag aagaagcctg cgatgactac aatgatacca tttagaatca
     3001 gcatgtagtt tccgactgtt tttccagttg tttgtgaaag tacaagagag atgatatcca
     3061 tcccaccagt tgtaaagccc atttttaaag ataatcctac gccaagacct aataaaacac
     3121 cgcctactag ggcattcatc aaaatattat cagtgatctg tccgactgga agcagcatcg
     3181 tcatcaccga tacacataca acattcacaa aactaaggat cgttgctcgt tttcctactt
     3241 tgagaaaccc taatacaaac acagggatat tcaaaagaaa aataaatgtt cctgtgtcta
     3301 tcttgatatg aaaccattca tataaaagcg aggaaatgat ttgagcgatc ccattcatcc
     3361 ctgcagaaaa gacattggct ggaatcaaaa aataatttaa agcgaccgca gcggtcaatc
     3421 cggttatcaa aataaccact attttcttta atctttcatt tttatgatac gcagtcataa
     3481 ttttactcac aagcacctct cccctacttt ggtattccaa atgtaagaat aacagtttat
     3541 ctattaaaat aaaatacttc gaactcaaat gagaagaatt ttagcaaaca ggtaagaatc
     3601 tgccctaatc ctatgataag atagatgtat ggtaaatatt tatatgtata tcaggagggt
     3661 tcctatggca aaagatgcta gcttcgacat tgtatcagaa gtcaatacag aagaagtaaa
     3721 aaatgcgatt cagcaaacaa caaaagaatt gtccaatcgc tttgacttca aggattctac
     3781 agtagaaatc aaattagaga acagcaatct agttgtattg agcgatgatg atttcaaaat
     3841 cgaacagatc aaagatgtat tatttggtaa gctgatcaaa agaaatgtgc caatcaaaaa
     3901 tatccatttt tccgaatcag aacacgctct aggtggcaaa gctcgacaaa ctgccgattt
     3961 ggtcaacgga atcgatcgtg aaaacgcgaa gaaaatcact acagctatca aaaatgaaaa
     4021 actgaaagta aaagcacaaa tccaagaaga tcagattaga gtgacaggta agaaccgtga
     4081 tgatctacaa gctgtgattg ctttattacg taaacttgac ttaccaatcg agttgcaatt
     4141 cactaactat cgttaatcaa aaaacgaggc tgacgtctgt cattcctcgt ttttttgatt
     4201 tactgcttct tttgataacc aaaaacgctg tattccttct tccaattccc caacacttcg
     4261 tacttctctt gctgtttgcc aatgttgtgg gaataaacgt cgataagaaa aactagaaaa
     4321 tcgttgatct gccaatacaa cgattccttg atccgccatg ccacgaatca ctcgaccacc
     4381 agcttgcaag actttgttca ttccaggaag ctgataagcg taagcaaatc caaggtgttc
     4441 cttctcatca taatatgatt tgatcagttc ctgctcatga ttcatctgcg gcaaacctac
     4501 acctacgatc atactgccga tcaatcggtc ccctctcaaa tcgattcctt ctgaaaaaat
     4561 cccgccaagt acgcaaaatc caatcaatgt ttcagcggga tttgtttgaa attttgttaa
     4621 aaatcgttcg cgttcttcct cattcatgtc cgtttcttgg attagcacat tgctgtcggg
     4681 atattgctgt ctgaatgctt ccaccgcaag atctaaatat tgataagaag gaaagaaaac
     4741 gaaataattc ccgacctttc catcagcaaa agcatgaatc gtctctacta attttgccag
     4801 actgtgttcc ctttttcgat aagtcgtctc taggtaattt gcgatgaaaa cttgctggtt
     4861 tccttctggg aaaggactcg gcagtttgta agccagactt ttttgtcccc ccagtgtttc
     4921 ctgataataa gagagtggtg aaaaactagc agaaaaaaga atactgcttt ttcctttatc
     4981 caacgactgc tcaagaaaca agcttggatc aatacaaaac tcttttacta tcagatcgcg
     5041 atacgttttt tcgactgttg tttcgtagtg atcatcataa aattcactga ccttcaaaaa
     5101 atgaagcaaa tcaaaataat aaggaagcaa ttgttcttgt gatgggtgct caggaaattc
     5161 tgccagccat tctttaattt tttcgcttaa ttgataacct gcctttacta aagattcagc
     5221 cggtgctttt tgatgatgat aatcccaatg atcttctttt gctatttctc ggatcgaatc
     5281 aaattctttt aatagtttgt tgaaacggcg atgaagtttt ttgaattcct ttggtatagc
     5341 ttccttactt cgtttcgttt tttcataaga tagttctgcc gaatacatct ctcgggaacg
     5401 attcaccaaa ttatgtgctt cgtcaatcaa aaacagatag tcctcgtttt tttcatcttc
     5461 aaaaaaacgt cttaaataaa cagttggatc aaataaataa ttgtaatccc caatgattac
     5521 gtcacaccag atactgacat ctaatgatag ttcgaaagga caaagtgtat gctttcttgc
     5581 ataagtttcg atgacttcac gagtgatctg attctcatga ttcaaaagat cccataaacc
     5641 ttcattaatc cgattgtaat aaccattggc atatgggcac tgatctggat tacaggtcgt
     5701 ctcatctaaa aagcagatct tgtctttcgc cgtcaaagtg acacttttta cttcagctcc
     5761 tgtatctttt aatgctgtca tagcatcttc agctacttgt ctagttattg ttttagccgt
     5821 tagataaaag atcttttctc cctcttcttc ccctacagct ttcaaagcgg ggaataacgt
     5881 agagattgtt tttcctgttc cggtaggtgc ttctacaaac aatttctgtt tcgtacggat
     5941 cgttttataa gcagctaccg ctagttcacg ctgacctttc ctatactcat catgggggaa
     6001 tttcaaagcc atcaaagagg cattgcgtac agtgcgccag ttttcttgaa aaacaagcca
     6061 ttctttataa tcttcagtca gctttttaaa aaaagtttct aactgctctc ttgtctgatg
     6121 ttcaaccttt ctcgtgatca cttcttctgc cgtctggaag taagtcaatt gcagatttat
     6181 ttcttgtaaa tcatgttctt gactatagat ataggcatac acccttgctt gatgaaagaa
     6241 aagttccact tgttctggtt ctaaatcttc aaatcgtggt tcggaggttt tgatttcatc
     6301 tatataataa ataccgtctc ttttgaaaat accgtctgca cggccttcaa cgatcaggta
     6361 atattcatct aattgaactg ttgtttttaa aaagacttct ttttcatagt tttctcccgc
     6421 atctttttgg agcttgcggt ggattctcgc tccttctaat gcagtatggt tacttttttt
     6481 acgattgtcg atacttccac gtcgcaaaat aaattctact aagtgccgca cagatatttt
     6541 tgactgtttg atcatttcca ccacctcttt ctctctaaga ttacctgaaa aaacctagtt
     6601 tagcaaaaag agctctcttt ccaaacataa aaaaacaccc tgctgattag gcagggatat
     6661 aggatataga atttggattt ttggatgaat ctaatgtaac aaggacatat taaaatgcta
     6721 tgaaaactta ctttccttct ctttagaata tccaaatact tttcatatag tctgataatt
     6781 cagataagta aagagaaata agtacctgtt tttgtttcct agcaatgaat gcgttatact
     6841 aatggatgaa agaagttttg gctatcaagg aggaatgcac atgcatatcg aaagattaaa
     6901 aacaggaatg attgaagaaa actgttattt ggtctacaac gaagaagcac ttttgatcat
     6961 tgaccctgga gaagatgcgg aacgaatcaa acaagaaatc aaaaaaacaa accaacagcc
     7021 ggtagcgatt ttgcttaccc atactcacta cgatcatatc ggtgcagtgg aacctttacg
     7081 cgcgtattat caaatcccag tttatgtcag cccactagag caaaaatggc taggtgatcc
     7141 gattttgaat ctttctggac tcggtagaca tgatgatatt gctgatgtta ttgtctcgcc
     7201 tgcagaatat gaatttgaaa tgaaaactta tactttagga aacatgtctt ttcgcgtcgt
     7261 ccctacccca gggcattcta taggaagcgt cagctttatt tttgatgatt ttgtcgtttc
     7321 tggcgacgcc ctgtttagag gaagtatcgg tagaaccgat ctgtatacag ggaatttaga
     7381 acaattactt catagtattc ggacccagct gtttgtatta cctgatgaat ttgcagtcta
     7441 tccaggacat ggcgatgcaa cgacgattga acaggaaaaa aggacaaatc cttttttcaa
     7501 tcgttaaaac gacttaggag gagaataaaa tgacagaaac tacactttat atcgtacgac
     7561 acggaaaaac gatgttcaat acaattgaac gagtacaagg ctggtgtgat acacccctca
     7621 caaagcaagg acaagaaggc attcattatt taggtcgcgg gttaaaaaat gtcgactttt
     7681 catttgctta ttctagtgac agtggtcgtg caatcgaaac tgcccgtatt attctaggag
     7741 aacattctaa gggaaaagaa attccttatt ttattgaaaa aaggattaga gaatggtgtt
     7801 tcgggtctct tgaaggtgga tatgacatgg aaatgtgggg tgtgatccct cgtgtattga
     7861 attttaaaac atatgatgaa atgtttacca cagaagtttc ttttgaacaa attgctaatg
     7921 ctgtatacaa cttagatacc gctgggtggg cggaaactta tgaaacattg cgagaccgtg
     7981 tatggacagg atttgaagat atcgcccatc actgcgaaaa aaatggtggt ggaaatgttt
     8041 tagtcgtatc tcatggtttg acgatcgcct ttttgcttaa tctgatcgat cctaaacagc
     8101 ctgttcgtgc tgggctttta aatggcagcg taacaaaggt cacttatgca aacggtcgat
     8161 tttctatcca cggcatcaat gatacgtctt atatcgataa cgggaaaaca catagtcgcg
     8221 actcgtttct ataatgggga tattttaatt tttaccagag aatcataaaa ggcaaagtct
     8281 gaacgagggc tttgtcttct tttttctctt cgtgtactta tctgctttat ttctcaagtt
     8341 tttcttaata ttaaaaaaca gattgggttt ctcaaaaaat aacggtatac tataagatgt
     8401 atcttaattg ggaaggaagg aatttatgtt ttttgcaaat attatcaaag cagtgattct
     8461 cggtattatt gaaggaataa cggaatggct gcccatcagt agtactggtc atttgatcct
     8521 agcagatgaa ttcatcaaac tggatgccag ttcacaattt atgtcgatgt tcaatgtcgt
     8581 tatccaatta ggggcgatct tggctgtcgt cgttctttat tttcacaagt taaacccatt
     8641 ttctccaagt aaatcaacgg tagaaaaaaa agatacgtgg acactttggt ttaaagtgat
     8701 cgttgcttgc ataccagcca tcatcattgg tttgcctttg gatgattggt tagaagcaaa
     8761 tttccacaag ttcttaccag ttgccattgt tttgatcgtt tacggtattg cctttattat
     8821 tgtcgaaaaa cgcaacaaaa acaaaacacc tcgttggact tctctgaatg actttacgta
     8881 ccaagcagct cttcttgttg ggttattcca agtcttggca cttgttcccg gaacatcccg
     8941 ttctggcgca acgatcctag gatctatctt gattggtgct tcgcgttttg tcgctacaga
     9001 gttttcattt ttcttaggga ttcctgtcat gtttggcgca agcggcttga agatcgtcaa
     9061 atatttagct gaagggaatt cattcaaaat ggaagaaact gttgttcttc ttgtaggaac
     9121 attcgtatct tttgtggttt ccgtcttagc tatcaaattc ttactaagct atttgaagaa
     9181 aaacgacttt accgcttttg gctggtatcg tattatcctt ggtatcattt tgattggtta
     9241 ttggtttatt gcaatgtaga aaaaaccaga acacttctct tatttgagaa atgttctggt
     9301 tttttatttt c
//
LOCUS       DAXWOO010000111         9001 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.112, whole genome shotgun sequence.
ACCESSION   DAXWOO010000111 DAXWOO010000000
VERSION     DAXWOO010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.112"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(69..1208)
                     /locus_tag="ACGE70_002040"
     CDS             complement(69..1208)
                     /locus_tag="ACGE70_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFF8693665.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1607..2905)
                     /gene="eno"
                     /locus_tag="ACGE70_002041"
     CDS             complement(1607..2905)
                     /gene="eno"
                     /locus_tag="ACGE70_002041"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFF8693666.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(3021..3776)
                     /gene="tpiA"
                     /locus_tag="ACGE70_002042"
     CDS             complement(3021..3776)
                     /gene="tpiA"
                     /locus_tag="ACGE70_002042"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFF8693667.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(3880..5070)
                     /gene="pgk"
                     /locus_tag="ACGE70_002043"
     CDS             complement(3880..5070)
                     /gene="pgk"
                     /locus_tag="ACGE70_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFF8693668.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            complement(5180..6181)
                     /gene="gap"
                     /locus_tag="ACGE70_002044"
     CDS             complement(5180..6181)
                     /gene="gap"
                     /locus_tag="ACGE70_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFF8693669.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            complement(6222..7313)
                     /locus_tag="ACGE70_002045"
     CDS             complement(6222..7313)
                     /locus_tag="ACGE70_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFF8693670.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            complement(7462..8796)
                     /gene="rpoN"
                     /locus_tag="ACGE70_002046"
     CDS             complement(7462..8796)
                     /gene="rpoN"
                     /locus_tag="ACGE70_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFF8693671.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            8856..8927
                     /locus_tag="ACGE70_002047"
     tRNA            8856..8927
                     /locus_tag="ACGE70_002047"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8889..8891,aa:Arg,seq:ccg)
BASE COUNT         2336 a         1905 c         1658 g         3102 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgtttatta
       61 aaaaaatctt acgctaagta ggataaaata attccttgat atttttcgat catatccaaa
      121 taattatcga tttcaacata ctcatctact tgatgaggca aagtaacgac accaggccca
      181 aagacgacaa agtcgaagga atggttagct ttcgtaaatt cggctgcatc cgttgttgct
      241 gccgctccaa ctaaaggtaa tggctggctg aattgttgct gaatgcaatg aatcaaagga
      301 gaatctggat cagctttgac aggtattttg ttgtaatcga tcatcaattc caaatgatag
      361 tctgtctctt gattcaattc attgacgatt gattgcagca atgcaatgat tttatcattt
      421 gggtattctg gaatcgatcg aatgttccct tggagctgcg catggcttgg gatgctgttg
      481 acttgattcc cgccactgat caatgtaaca ttatggattg tttttcctag gacaggattc
      541 tcgattgttt cagcgaggtg attcatttca gcatttgctt tcgtaatgaa ttcatttagg
      601 tgattgattg cattataacc ttggtctggc atagagctat gcgcttcttt tccgtgcgag
      661 gtgactgtat agttgattga tcccatgtgc gtatacatca aactgtaatt ggttggctct
      721 ccgatgatca aggcatctaa gtcatctaca tagcctgctt tcgtgagctg ttccccacct
      781 agttcaccca cttcttctcc gacagtggct aataatttga ctgttccgtt aaatggtttt
      841 cctgactctt tcagttcgat catggcaatc accattgctg ccaagccact cttcatatct
      901 gtagcaccac gtccgtacaa acggttccca tggatttctg ctgcaaaggg tgcataggtc
      961 caagacgatt catcaccagc cgccacgaca tccatatgcc cagataggcc taacactttt
     1021 cctgattggc ccttttggta acgagcaatc aaattatctc taccatctct gtaagaaaca
     1081 atctctcctg ttatgtcatg ctttgccaaa agcttattca aataggctgc tacttcacct
     1141 tcgtttccat tcactgattt aattcggata atctcttgta aaatcgcgat tttttcttct
     1201 tttttcatct aatcctctcc cttgccattt ctttttattg tatttgagtg tgctcgaatt
     1261 ttcaaatagg agcaaccaaa gtaaaaatga agaaagagag ccactttatg aaaaatatgc
     1321 tgctacttcc ctcctatttt atttatacga gaggacttgc ccatagattc ctattctact
     1381 gttctatcct taaaagctct tatatgatgc tagttattgt tagctttcct ttttcaagga
     1441 tgatcttttc ttgtaccatt ctttgtaaac atcatataca gaagctagtt gcaaaaaaac
     1501 tctctacctg ctgatatttc agcaagtaga gagttcgatt acttaactaa gtgatcatac
     1561 tcgactatct ttcaatagtc gatcatgtat cttcattaga aaagaattat ttgtttttta
     1621 agttgtagaa agatttcaaa cctttgtatt ctgcaacttc accaagttgg tcttcgatac
     1681 gtagtaattg gttgtattta gcaatacggt cagtacgtga tagagaacca gttttgattt
     1741 ggccagcgtt tgttgcaaca gcgatatcag agattgttga atcttctgtt tcaccagaac
     1801 ggtgagatac aactgctgtg tagccagctt ctttagccat ttcgatagct tcgaaagttt
     1861 ctgttaatgt accgatttgg ttcactttga taaggattga gttagcaatt cctttttcga
     1921 taccttcaga taatttttga gtgtttgtta cgaacaagtc gtcaccaact aattgaactt
     1981 tgtcgcctaa tacgtcagtt aatttcttga atccgtccca gtcgttttcg tctaatccat
     2041 cttcgattga gatgattgga tatttagaaa ctaattcttc gtagaatttg atcatttcgt
     2101 cagttgtttt ttcgccttcg cctgagtcag ctagtacgta aacacctttt tctttgtcgt
     2161 agaattctga agaagcagca tccatagcaa gaacaacgtc tttaccaggt acatagccag
     2221 ctttttcgat tgcttcgatg attacttcaa aaccttcttc gtttgaacca aggtttggag
     2281 caaatccacc ttcgtcacca acagaagttg ctaatccgcg agctttcaag attgaagcta
     2341 atgcgtggaa tacttcagca cccatacgta gagcttcttt gaatgttgga gcgccaacag
     2401 gcatgatcat gaattcttgg aagtcgattg agttatcagc atgtgatccg ccgttgatga
     2461 tgttcatcat tggtgttggc aatacttttg tgttgaatcc gcctaggtag tggtataaag
     2521 gtacttctag gtagtcagca gctgcacgtg caacagcgat agaaacacct aaaatagcgt
     2581 tcgcacctaa tttacctttg ttaggagttc cgtctaaagc gatcatagct ttgtcgatag
     2641 ccatttggtc acgaacatcg tagccaatga ttgcttcagc aatgatgttg tttacgttgt
     2701 cgacagcttt agttacacct ttaccgccat aacgagcttt gtctccgtca cgtaattcaa
     2761 ctgcttcgta ttcaccagtt gaagcacctg aaggaaccat tccgcggcca aaagctccgc
     2821 tttctgtgta tacttctact tcgattgttg ggttaccacg tgagtctaag atctcgcgag
     2881 cgtaaacatc agtaataatt gacatgtttt gtctctcctt tgagtttgtt ttttcctaag
     2941 ggatttccct catatacgat tttagttaat tttgctcatt agtgcaaaca tttcttgcat
     3001 ttttcgcaaa ttttcaacaa ttattttaca gcgtctaaca atgacaagaa tgaatctgct
     3061 tgcaagcttg cgccaccgac taaagcaccg tcaacatttt cttttgccat gtattcagca
     3121 atattttctg gttttacaga accgccgtat tggatacgaa ctgcttctga tacttctttt
     3181 ccataaagtt tttcaactgt ttgacgtaca actccacaaa tttcgtcagc gatttgtgcg
     3241 tcagctgatt taccagtacc gattgcccag attggttcat aagcaataac catgtttgac
     3301 acctgttcag ctgaaagatc agctaaacca gctgtaattt gtccttcgat ccattcagca
     3361 gttttgcctg cttcataggt ttctaaagat tcgccgcagc ataggattgg tgtcatacca
     3421 tttgcaaaga tagctttcgc ttttttattg atgtcttcgt ctgtttcatg gaaatattca
     3481 cgacgttctg aatgtccgat gatcacataa tgaacaccta gatcagcaag agctgctggt
     3541 gaagtttcac cagtgaacgc acctgagttt tcccagtagc agttttgtgc tgagattttt
     3601 agttctgtac cttcagctga tgtcattaat tcttgtaaga ataatgcagg agatccgatg
     3661 actgaatcaa ctttgtcatt tgaagggatt ttgtttttta ctgcttcagc aaattctttt
     3721 gcttctgatg cagttttgtt cattttccag ttacctgcga taataggttt acgcattgag
     3781 gaacacactc cttaatttat tgaaaaatgg ctagggagtc atgaaaactc ccttcacctt
     3841 ttgttcgatt actcgtcaga attgagcaaa cttggtcgtt tatttatcgt tgattgctgc
     3901 tagtcctggt aattctttac cttcaagcaa ttccaagctt gcgccgccac ctgttgagat
     3961 gtgtgtgaat ttgtcagcaa atcctaattg ttctgctgct gcggctgagt ctccgccacc
     4021 gatgattgtt gttgcgcctt ctaagttagc gattgcttca caaacaccga ttgtaccttt
     4081 agcaaagtta ctcatttcaa atacacccat aggtccgttc caaacaactg tttttgcacc
     4141 ttgtaatgta tcagcaaata attttactga tgctgggccg atgtctaaag ccatcaaacc
     4201 atcaggaatg tctccttcga ttacttgtgt ttcaacatca tttgaaaatt ctggtgcaca
     4261 gacattgtcg attggcaaga caagtttgtc gcctgctttt tcgattaatt cttttgctag
     4321 ttccacttta tcagcttcta ctagagagtt accgatcttg attccttttg cttcgtagaa
     4381 tgtgtaagtc attccgccac cgatcaagat tttatctgct ttaggaatca agttttcgat
     4441 tacgccgatt ttatcagaaa cttttgcgcc accaaggatt gcaatcattg gtcgttttgg
     4501 ttcttctact gcttcaccga tgaacttgat ttctttttcc atcaagaatc cagcaactgt
     4561 tgggatacca gttgaagcga tacctacgtt agaagcatgt gcacggtgag ctgtaccgaa
     4621 agcatcgtta acaaatacat cgcctaatga agcccagtat ttacctagtt ctgtatcatt
     4681 tttgctttct tttttaccgt caatatcttc aaatcgtgta ttttcgaata cgacaacgtc
     4741 gccgtcattc atgttgcgga ttgcttcttc taattgttct ccgcgagttt caggtacgaa
     4801 tgttacttct ttacctaata attcgcctag acgttttgca actggagcta aagattttcc
     4861 ttctttatct tcttctgttt ttacacgtcc aaggtgagag aaaaggattg cttttccgcc
     4921 ttgttctaga acgtagttga ttgttggcaa tgctgctttg atacgagtgt cgtcagtgat
     4981 cacgccgtct ttcaaaggaa cgttgaagtc aacacggaca agaacttttt tgtcttttaa
     5041 atcgatatct ttcactgttt tcttagccat tttatttcct cctgaataaa cttattatca
     5101 aaaaaagcgg aagaagcgcg attgcttccc cgcttttaaa tgtacatgtc tataatttac
     5161 acttgtacgt ttgtctatct tataagttag cgaagtattc taaagtacgt actaattgtg
     5221 cagtgtatga catttcgttg tcgtaccaag caacagtttt aactaattgt ttgtcgccaa
     5281 ctgtcattac tttagtttgt gtagcatcaa ataatgaacc gtaagtcata ccaacgatat
     5341 ctgaagaaac gatttcgtct gtgttgtatc cgtaagattc gtttgaagct tctttcattg
     5401 ctgcattgat ttcgtcaaca gttacttctt tttcaagaac tgttactaat tctgttaatg
     5461 aacctgttgg tacaggaaca cgttgagcag ctccgtctaa tttaccgttc aattcgggaa
     5521 ttactaaacc gatagcttta gcagcacctg ttgagttagg aacgatgttt gcagcagcag
     5581 cgcgtgcacg gcggaagtca cctttaggat gaggtccgtc tagagtcatt tggtcacctg
     5641 tgtaagcgtg gatagttgtc attaatcctt caacaacacc aaatttgtcg ttcaatgttt
     5701 tagccatagg agctaaacag ttagtagtac atgaagcacc tgagataact gtttctttac
     5761 cagttaatgt ttcgtggttt gtgttataaa cgattgttgg aacatcgttt ccgccaggag
     5821 cagagataac aacgcgttta gcaccagctg ttaaatgttt ttcagctgct gatttagaag
     5881 tgaagaaacc agtacattct agaacgatat ctacgcctag ttcgccccat ggtaattctt
     5941 ctgggttgcg gttagctaat actttaactt cttttccgtt tacgttgaat gaaccttcat
     6001 gaacttcaac tgttccgttg aaacgtcctt gagttgtgtc gtattttaac aagtgtgcca
     6061 acattttagc atctgtcaag tcgttgattg ctactacttc gattccatct acatcttgga
     6121 tacgacggaa tgctagacgt ccaatacgtc caaaaccatt aatacctact ttaactgtca
     6181 tttaagattt cctccttatg aaaatcaaaa aaatatttat tttaaagggt tacccctttt
     6241 aaaatctcat ttgcagcagc ttcatcagtg atgagccacg tttgttttgg tgcatttttc
     6301 atataagctc gaattgcttt agcttttgat tttccgcccg cgattgccat gatgatcggg
     6361 actttttcca aatcttgcaa ttctatgccg attctaggga ccttataaac gacttcacct
     6421 ttttcattga aaaagtagcc aaaagattcc gcaacagcat ttgcttgttt cagcatgact
     6481 aattcttttt cagtcatctt acgacgtgct gccatgtgta atgcacgccc aatactatga
     6541 ataacacaat tcgcttcact aatcaagttt aagacttctt ggatagatgg ctcattgagt
     6601 aaagaattgt aagttgccag acttaattgt tccggaacat aaagtgcgcg gtggttaccg
     6661 tttgctttag cagccattct tgcactgaca gaatttgcct gtacagttat cgcttcacca
     6721 atcccgcctc ttgcagggac aaacaggtta tgccgtttct tattttccag attagacaat
     6781 tgttcggcta ccacagccat agtagttcct cccatcacag cgatgatatt ctcgccttct
     6841 ggaagtagtc ggtctaaggc ttcgctcagc gcttcgccga atgcatcagc cactttggtc
     6901 tgctcttcgc tgtttcctga aacaacgata cagcgttggt taccaaaata ttctgccaac
     6961 tgttgttcgg tttgatgcgt acctaaaagc tgatccatga agttttcaag gctttgataa
     7021 acttcttcgc ccttagttgt cagcgtcata ccgctttttg aggtgtcgat caaattcaga
     7081 tttttcaaaa ggtctgtttc tgttcgtaag actcgttctg tgatccccat actttccgat
     7141 aaacttcgac gcccaatcgg ctgcatccaa tagatatttc gtaaaatttt atagcgttct
     7201 tgcaacactt cgataatatc tggtgcaaca gattcaatca tttttatttc atccagcata
     7261 gcgatctcct tagttggaca ctttacgtcc aatgtagact taaaacgacc catatacgat
     7321 aaaaaaagaa attaactatt cgaatttcac atcaataaag ggaaatctca acctcacgac
     7381 tgtaagtcta acagttttgc tctcgttttt caactaaaaa gactagaaaa agtgaaatac
     7441 tttttctagt cttttgactg attagttatc ataacgtttt ctttttgatg aactcgggat
     7501 tcctaataaa tcacgatatt ttgctactgt tcgtcttgag atcgctatct catctttttt
     7561 cagcaattca accaatttct ggtcagacaa cggcttgttt ttgtcttctt gatcgactaa
     7621 ttcctgcagc tttttcttcg ctgtatctgc tgaaaggtct tctgtctgtt ctgtactatt
     7681 cgtaggaatt cgtgtagtga aaaatttttt taattcaaat acaccgaagt ctgtttccaa
     7741 atatttcccg ttgactgcac gactgaccgt ggattcatgc acatctatct cagttgcaac
     7801 ttcctttaac gtaagaggct tgattggacg ttccttgtcg ataaagaaag ctttttgccg
     7861 ggaaacaatg acttgagcaa cccgcaaaat tgtatcccca cgttgtagga tcgttttctt
     7921 caaccattcg aattcctgtt tccgttcttt taaataattt tcagtttctt tatctgcttg
     7981 ttgtgacatt tgtttgaagt aactctcttg aaagcgtata tttggctgat tttttcggtt
     8041 tgagataact tgtacatttt tatcgtcgtc cactaatacc cggacatcag gaataatgta
     8101 caattcgctg ctacggtcga aaatccggcc agggcttgga ctcaacgttt gtaaatagtc
     8161 gaatatctgc tgaacagcat gtagatcaac agaaaaacgt tgtgcgattt ctttccattt
     8221 tcgctccacc agttcctcaa aacattcttc caacacgata taagctaatt ccggcgcata
     8281 atcatctcgt tctgtttgaa gcatgagaca ttcttgcaaa ctacgtgcac ctactccagc
     8341 tggttctaat tgctgaatca gagtcaacgc atcgagcatt tggataggcg tagctccaat
     8401 ctcttttccg gcttcttcta aactaatcgt caaaaatccg ttcaaatcga tgtattctac
     8461 aagataaaga acgattttcc tcaaaaaagt atctcggtaa ttcaaatgga cttgttcgat
     8521 caatgaatca aataaagacc cttttgtatc aggaatctga tttaggtaat ttgtctcttc
     8581 tccttcataa gaagatgaag aagcttttat gtaatctggc tgccattccg gctcaacaac
     8641 ttccaccaat ggattttcca ttgcttgatt ctcaacgaat gccaacagct cctcactgtt
     8701 atattgtaaa acttggatgg actgttgcaa ctgttgcgtc atggctagtt tctgtgtctg
     8761 ctgctgtttt tgtgaaaaat tttgctcaaa tttcattttt ttgtccaatc cccttgtaat
     8821 tttttatctg tttagttaca attatcccag tgcttgcgtc cttagtgtag tggatatcac
     8881 gtaagattcc ggttcttgag acgggggttc gattccctca ggacgcgttc tactgctatt
     8941 ttagcataat ttacgaatag ttgcttcttt atttttaagt gaaaactgtc cgccaaagaa
     9001 a
//
LOCUS       DAXWOO010000112         8900 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.113, whole genome shotgun sequence.
ACCESSION   DAXWOO010000112 DAXWOO010000000
VERSION     DAXWOO010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.113"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            74..1414
                     /locus_tag="ACGE70_002048"
     CDS             74..1414
                     /locus_tag="ACGE70_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFF8693672.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1632..2075
                     /locus_tag="ACGE70_002049"
     CDS             1632..2075
                     /locus_tag="ACGE70_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFF8693673.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(2579..3997)
                     /gene="nhaC"
                     /locus_tag="ACGE70_002050"
     CDS             complement(2579..3997)
                     /gene="nhaC"
                     /locus_tag="ACGE70_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFF8693674.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(4400..5623)
                     /locus_tag="ACGE70_002051"
     CDS             complement(4400..5623)
                     /locus_tag="ACGE70_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFF8693675.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(5654..6493)
                     /locus_tag="ACGE70_002052"
     CDS             complement(5654..6493)
                     /locus_tag="ACGE70_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFF8693676.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(6510..7190)
                     /locus_tag="ACGE70_002053"
     CDS             complement(6510..7190)
                     /locus_tag="ACGE70_002053"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFF8693677.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(7192..8265)
                     /locus_tag="ACGE70_002054"
     CDS             complement(7192..8265)
                     /locus_tag="ACGE70_002054"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFF8693678.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(8321..8559)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(8602..8835)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         2553 a         1817 c         1619 g         2911 t
ORIGIN      
        1 tagtcattgt taaatattgc tatttattct taatataaat tttgattgtg aatttaaaag
       61 aaagaggtca tttatggaag ataaaaaatt gaaaaaacaa cttagctcca gacatatcac
      121 catgttagct ttaggtggag caatcggtgc aggtctattt aaaggaagtg gcgaagcgat
      181 tgggatcgcc ggcccttctg tattgattgc ttttttgatt ggtgggctga ttttatatat
      241 cgtgatgaaa ggtctgggaa aaatcgtttt aagcggtggc gacacgcatc acggattatc
      301 tggtttgata cgcccttatc taggtgctca ttctgctgat tttactgatt gggtctactg
      361 gtctatgtgg attatcaata tcatcgctga agctgttgca gctgcttcct ttttgcagtt
      421 gtggttcccg cacattccag cctgggtatt tgtttttatg ttggctgttt taactacgat
      481 catcaatctt tattcggtac ggttattcgc agaaacagaa tattggctgg cttttgctaa
      541 aatcagtgtc attatcttac tgatcatttt tgctacttat cttgtcggtc agcaaatgtt
      601 gggcacaggc gttttcccta cccttcagca attgactgac catggtgggt ttactcctca
      661 tggtatgaag ggaatcatca gttctttgct tgtagttatc tattcttacg gcggttctga
      721 actgattgct atcacggtaa gcgaagcaga tgatcctaaa cgtgcgattc ctaaagcaat
      781 caaaggtgtc atgggaagaa tcatttcctt ttacatcatt cctttgtttt tgcttttgat
      841 tctttttcct tggaatactt tagcaggcac gaatgtcagt ccttttgtga tggtatttga
      901 aaaaatgaat atcccatttg cagcggatat cgtcaatttt gtgattgtcc ttgctttgtt
      961 ctcatctatc aattcgggtg tgtatgcatc ttcaagaatt ttgttcttcc gtttgaagga
     1021 tcgaaaaggt tcctctagaa aactagctgt tttaaacaag catcaagtac cgcaacgtgc
     1081 tgttttattt tgtaccagtg ttctttatct tggtgtagct ctttcttatt ttgtcggtga
     1141 caaattattt ggctatctgg caggttccct ttcgtataca gtattattga tctggatcat
     1201 tatcagtgcg gcatcctttg tactggcact aagaaaagga acattatggg ataaaggaat
     1261 cagctttttt gcattagctg tcttaggatt gattttcctt ggtatcctgt tcacgaactc
     1321 cattggggta actgtattga ctggcctgct ttacctgttc atttatttca gttatcaaaa
     1381 aaagaatgat gcgttcgtat tgacgaatga atgaaatcga tagaaaattt tattttattt
     1441 atgtggaata gatggagcta ttgaagaaag gagttgtgac aaaagtcttg ttgcaactcc
     1501 tttttccgaa taaacagtct catgacctct tggtaaacgg tgttcaaaaa ccctctgtgc
     1561 aaaataattg attgttctcc atgctcatga catgatagaa gaaaggaatg aaaaaaagga
     1621 gtttgtttta aatgaaaaag tattggaagt ttttgttgat gatcggtgtt tcaacggtag
     1681 ttatgtttgg tatgatgtac ttgaatgtct atgcgctcga tcatatcttc ttcagtcgta
     1741 cacgagtatt catggcgttg atgatgggtg cagtcatggc agtcatcatg ctgttgttca
     1801 tgtggaaaat gtatgaaaac aaaaagataa atgttatgat cttagccgct agtcttcttg
     1861 tttttgccgg ttccttgttt atggtacgaa gccaaactac tgtcaacgac acagcttgga
     1921 tgaaggccat gattcctcat cattctatcg ctatcctggc aagtaaacga gctgatttaa
     1981 aagatcctga agtaaaaaaa ttagcggaag aaatcattca ggcacaagaa gaagagatcg
     2041 acaaaatgaa aaaaatgatc aaagaaatgg aatgaccatt atccgtccaa ataaaaaaag
     2101 aggatgttcc agaagttatc agcttcaaga aataagaaga agctacttct ggaacgccgt
     2161 gaataagggg ttgtgaaaaa gctgccctgc atcaagtaat aagaacagcc aaacaaaaat
     2221 agcttctcct atttttcgtt tggttaggct tattaccgca gatgcaagct tttgaacacc
     2281 gtttattaag aggttgtaaa atcagcgttc tgacctgagt attagagaga caaatggaaa
     2341 aatagttcct catattttgc cattttttga gctaataccg agggtcagct gattgaacac
     2401 cgtttatcga gaggttgtga aaaagtcgct tagtttcgcg tattaagaaa aattttgtga
     2461 aatggctttc cacatttttg tataaatttg gcttaatacc gagaaactgg cttttgaata
     2521 ccgtttattc gtaaaaggga ttgtgacaaa agtcttgtca caatcccttt ttatgttttt
     2581 attgttctaa ttgcgctgtc ttgcttactg cttcttgcgg tttcacaaat ttttttgaga
     2641 ttccccaacc tgtgaaggca ctgaccatcg aaaacacagg agaaagcaaa ctgaaaaata
     2701 cgaatggcaa gtaagtgagt gtaggaatac caaatgtact tgctacgaaa cttccggcaa
     2761 ttccccaagg cactaagtag ttgattactg ttccaccatc ttctagcgtt ctacctaata
     2821 cagttggatc aaatcctgct ttttcataag tttctttaaa tgcattacca ggcagaataa
     2881 cagataggaa ttgttcacca ataaaaatat ttactccgat acttgtcatc aatgttgtaa
     2941 aaatcaaatt tggtgtgttc gtcatctttc ttgatactcg ttccatcacg gttgagatca
     3001 agccaaattc gatcaacaat ccacctaatg acaaagttag aataatcaat gacacagtag
     3061 gcatcatact ttcgattcct ccgcggctaa gcagtgaatc gatttgttgg tttccagtat
     3121 ttgaaacaaa ccctgattcg atgaccccag ccaacttcgt taaggaagtt tgtgggtttt
     3181 gaataaaaat cattcctact gctacagcga tattcaacaa aatcgttaaa attgccggca
     3241 cctttttcca tgcacacagt aacattaaaa ataaaggaat cagtgaccag attgaaatag
     3301 taaagtttgc ttctagaaca tcagtcactt cacgaataga tgaaagactt gcggctttac
     3361 ttgtgtgtcc taaaatcatg aataaaaaga aagaaacaac aaatgccggg acagttgacc
     3421 acatcatatg cttgatgtgt ttgaacaaat cggcatcaac aatagctgct gctagatttg
     3481 ttgattcgga taatggagac atcttatctc caaaaacggc acctgaaaca atagctccaa
     3541 caaccagcgc tggatttaag cctagtgttg tcccaatacc gaaaaatgca atcccaatcg
     3601 ttgacataac agtaaaggca ctccctacag cgctacctac gattgcacac acaacaaata
     3661 cagaagggac aaaccactgg acactgatca atttgaaccc aatgaccatt agagttggaa
     3721 tgatccctgc ttgtatccat acagcaatca atgaaccgac taaaataaaa ataaagattg
     3781 ggatgattcc tggtttgatg ccattgatga ttccttcgtg cagccaatct gtcgggtatc
     3841 tttttatcac tgcatacaac atgaccattc cgatcgctgc taagatcgtg atattaggtg
     3901 agacctttaa tccgacaacg ccaatcgcaa tgcctaaaac aataaccatt aatacaagtg
     3961 ttgcttctaa aaaacttggt gttccttttt ttcccatgat aatctctcct tttttagttg
     4021 ttcttatgtt ctaaaaaagt taagatatcg gattactccg ccgcaattca tccgcgattc
     4081 ctcctttata aaaataaaaa gacctgtttt aaactaagga cgaatgactc cgcggtacca
     4141 ccttaattaa aaacagatct gtttttcact ttcaaacttt tattcaaatg tatggacttt
     4201 ccatgtaatt cgtcaattag gcttgatcgg ttcacagcga ccaccgactt tctgaacaaa
     4261 acacctaacc gctactgcgt gaaaagttga cttgtacgaa gtttaccttc cataagatca
     4321 tttgtcaatg aaaaagaaaa tattttttca tttttctgaa aattattttt ataactttca
     4381 cacacaagtt tattttttac tagaaagtag cgccctcttg tttttttctt gctactattt
     4441 cttgatgttc ttgcttgatt ctttctaata gctttggctc tttcaacaga tcaagagctg
     4501 tattggcgag tagttctgct gcaatcgcaa tagaagcaag gcctttgtca cttttagcag
     4561 cagctttgaa ttcttctgtg tgtccggcaa tatatgtctc agagattgct accgtaggct
     4621 ggattgttgg tatgacttgg ctgacatttc ctacatcaga agaaccgaga cttgttcttt
     4681 gttccgtttc tatttcttca tctggaacac ctgcttcttt tgcatgctgg aagaataagt
     4741 catcaaaaga aggagtaacg atgacatcat caactgcatt ttggaacaat ccaaactcaa
     4801 aagtcgtgcc cgtacttatc gcagcacctc gtacgatatt ttccactttt tcatatactt
     4861 tatctaatgt ttgccgattt cctgcacgta aatagaaacg tccagcagcg taatctggaa
     4921 tgacgttggc agctactcct ccgtctgtaa tgatcccatg gatattgaca tcttttggca
     4981 attgcaagcg tagagcgttg attccattaa acacttggat caatgcttcc aaagcattta
     5041 ctcctttttc aggtgctgct gctgcatggg aagcgacacc gtaaaatttg atgtcgactg
     5101 gatcattcgc taaagattct gtcgttttcc cataacgaaa agctggatgg acgcataacg
     5161 ctacatcgac atcctcaaaa aaaccttctc gaacaaagct gcctttagct gatccgttct
     5221 cgcccccttc ttcccctggg gtgccatata cacggatttc tccaccagtt tcatcaatga
     5281 attgcttcac tatgctggcg gctaatacgg agtaagtacc aaacaaatta tgcccgcatg
     5341 catgaccaat acctggtaaa gcatcgaatt ctgccaaaaa ggcaattgtg ggaccgggtt
     5401 tattggaaat ataacgtgca tcaaaggctg ttcggtgtcc agctacttct ttttctaccc
     5461 aaaacctttc tttttctaat tgtttaatca acgctgctga ggaataaaac tcatagttac
     5521 ttacttcagg atgttcgtgg atatctagag caagtgcttg atattctggt aaacgatatt
     5581 ggattagatc cttcaattgg tttgttgaga ttgttgttgt cataaaaaat tcctcctatg
     5641 ctagtcattt ttcttatttt ctcccgaatg tttcccatgc aggcacgctt gatccttttg
     5701 atgttttctc gattactttg actgtgtctt ctgtttgata agcgtctact attttttgat
     5761 acgttttatt gttttcatct tcttttcttg caacgatgat attcacataa ggacgtgcat
     5821 ggtcatccac tggttctaag aagatggcat cagaagcagg attaaatcca gcatctacag
     5881 ctacaccgct attgatcact gaaatgtcta catctgacaa agcacgtgct gtttgggaag
     5941 catctaattc agaaatgtct agattttttc tattctcggt aatatcagaa acagtgggcg
     6001 cttgtccttt tgccttatcc actttgatca atccagcagt ttgcaataac agaagagctc
     6061 tgccgccatt tgtaacatca ttagggattg ctacttttgc tccgtctttg atatcagcta
     6121 catccttcaa agtgttagaa taaattccca gcggtgcatt gacagtattt ccgatcgata
     6181 ctaaatcagt tccgtgttct ttattgtaat tgtctaaaaa gaattgatgt tggaaagaat
     6241 tcaaatcaat cgatccatct gctaaagcca catttggttg attgtaatct gtaaatttca
     6301 cgtattccaa attaatgttc tcctttttca aacggtcttt cacgttgtcc catacatctg
     6361 tatcttctcc aataatccct agttttacga tagtttcttt tttgtcagtt gtatctgctt
     6421 ttccttggct acaccctgcc aaccaaataa ttgctacaaa tgtaatgact acaaaacgaa
     6481 taattttttt cattttttct cctccacttt caatgtgtaa tttttttaat cagttgatta
     6541 ctgataaatt gacttaaaaa aactaagatt agaatcaata atgtagccac aatcgttaca
     6601 tctgtttgga atcgattata ccctctcgat atagcaagat tccctaatcc gcctgcacct
     6661 actgctccag ccatggtagt cagtccgatc acgttgatga tagtcaacgc cgagacccga
     6721 atgataccag gcaaaccttc taacaaatac acacgtaaaa tgataccgat tggactggtt
     6781 cccatcgctt ctgccgcttc gatcacccca ggatcaactt ctagcagtgc gttctcgatt
     6841 tgacgagcaa aaaacggaac aactccaaaa atcaatggca ctaaagctgc tttcgcacca
     6901 atcgtcgttc caactaagaa acgagtagtc aatgctaaaa gagaaagcaa gataatgaaa
     6961 gggatggaac gaatgatatt gatcagctta tcaagtattt caaatagcag acggttagct
     7021 agtaatccat tagggcgtgt cactactaac gtcacaccta agatgattcc aatcatcccg
     7081 ccaatgaatg ctgtccaaaa agacatatat agtgtttgct tcgtcgcttc gacaaattca
     7141 tccggaatca aataaacatt gggcaaatat gttttaaaga aatcagtcat tttatcttcc
     7201 tccttcgatc actttgatac gactatctaa atcgtttttt tgtaaaatat ttatttttac
     7261 accagatttg atcaaataat ccaatgcttt tttccgctgt ttggtttctc cagctaaagt
     7321 aacgatcaaa ctccctaacg gcgtttcttg taataactct acattgccat ataaaatatt
     7381 cgctgttact tgatatttgc tgtacagatg agcgattaga ggttcgttcg tctgatttcc
     7441 aatataactt aattccacca gccatttatt ggcgatctga tcggcaaaag cagaatgggc
     7501 aatgatcgtt tctaatgctt ggtcaagatg agtagctgtt cgaataaaat ctttggtcaa
     7561 ttcttcttca ggattgctga agatctggat gctgctacct ttttcaacga ttcttccatc
     7621 ttccataacc gctaccttgt tgcagatttc tttgacaact tgcatctcat gagtgatcag
     7681 gacgattgtt aaacctaact ggcgattcaa tttttttaac aatgcaagga tctgaagggt
     7741 cgttttcgga tcaagtgcac tggttgcttc atcacataat aataccttag gttcattcgc
     7801 caaagctcgt gcgattgcta ctcgttgttt ttgtcccccc gacagttgtt taggataagc
     7861 agatgctttt tcttccaaac cgacaagttc cagtagttca ttcacttttt gacgacgttc
     7921 ctgtttcgat tttccagaat actttaaaga aaaatcaacg ttgtcaaaaa tagttcggct
     7981 ttccattaga ttgaaatgct gaaagatcat accgatggcc tttctttctt ttcgcaattc
     8041 tttgggagag agctgactca gattcgtctg attgacaatg acttctcctg ctgttggttt
     8101 ctgcagcaaa ttcatcacac gaactaacgt acttttccct gcacctgagt aaccgacgat
     8161 tccataaaca tcgccttttt caatcgttaa atctacagaa tctactgcct gtatctcttt
     8221 ttctttttgt ttgaaacata cagaaacatc tcgcaattca atcattaaaa ttcctcctta
     8281 tctaaaaaaa gcgtcctcaa gaaaatgaaa atgtcatttt cttgaggacg cttttttgcg
     8341 tgttaccacc tcatgttcaa gctgtgttca cacacaactc ctcttcaagt acgatcatac
     8401 tctagcaaga tatcggtcgc agccgtcatc gccttacatc tatgctcggg atgaattgct
     8461 cgatggccat tttccaatag tcgttccttg cttcttctca gctaccgaag ctctctgtca
     8521 aaggttcttc tatcgtactt accatttcaa tgcaaataat attcaaaaaa aaagtccttt
     8581 tactaacata aactatgtca gtaaaaggac gacgtatcgc ggtaccacct tttttcggaa
     8641 gtttccttcc ctcaagccca acatctcaaa aagagagatt ggcgttcgtt ttaacggtcg
     8701 aacagttccg aatttgccta cttatcgaca aattattctc caaggccatt ttcagctttt
     8761 cttacttgac tcttctcacc agccagagcc tctctgcaaa gtttccaaac ttactttcct
     8821 tttcaacgaa atcgatattt tgttgttggg attaatctac atgaggaaaa ctgaaaagtc
     8881 aagtacgaaa ttcaaattat
//
LOCUS       DAXWOO010000113         8867 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.114, whole genome shotgun sequence.
ACCESSION   DAXWOO010000113 DAXWOO010000000
VERSION     DAXWOO010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8867
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.114"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..606
                     /locus_tag="ACGE70_002055"
     CDS             <1..606
                     /locus_tag="ACGE70_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693679.1"
                     /translation="KEQEEKQRQKEKEREQLEIRKREQDLAEQKMAIEKQRLEQEKEK
                     EAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQANLTPSPQRSFNGWMVGCI
                     ASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQEQARKEYEQQLEELLNRTNQ
                     STTESSMENSKESDSSKAKEGSSDSTVQSNTSESSESNEVK"
     gene            606..3218
                     /locus_tag="ACGE70_002056"
     CDS             606..3218
                     /locus_tag="ACGE70_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFF8693680.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPENEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHKGK
                     KFHEERVKVGYRGFCEINFDCHLEQGDHVCLKYNHQGKQYVAWYEFSQKVLKDEEGKI
                     VYRKKRGETHLPELEKEAVLTLDEKKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSFENETAKKLLIQFYEEQATDRLQEYVEKLIKNEEISEMI
                     RKRLLLEAEKAVKE"
     gene            3215..5593
                     /locus_tag="ACGE70_002057"
     CDS             3215..5593
                     /locus_tag="ACGE70_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFF8693681.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PDQAKKMSESSDISTVQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYRRY
                     ISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSAIE
                     DISQSFLLLAFTILELRIYTLMLSGIGDLHAVGKINGFLYVVALIVLTIALFKGSQSV
                     TKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGFGH
                     RKDITHDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGTVE
                     DLTEKAKDTLEEKLDGTRAKEFAEAVKNPSEALEKQKEKASAVKEGLKDAYGEGQVNA
                     AVKANTTSEKENQEGAAIPSAKDHQDELHSNENSIGEKDSEEGQHGQLPKTDPEQYRY
                     KSNINSENEKRISDLPKRNQEVQTDEDSIDLPNDNGIEVGTEKSTKIGLPKTQNLPMK
                     DSRLNQFKENDQSFVHQSPAGTVQMKSSRLPENGNLTTVSYNLAESGETENASVQNIV
                     TKEAAPKPINKVHNGYRPANVAFTAPTKINVTRETKNRNNELKEQLLKDIKP"
     gene            5654..6196
                     /locus_tag="ACGE70_002058"
     CDS             5654..6196
                     /locus_tag="ACGE70_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693682.1"
                     /translation="MEDILIATFITALGAFLTIFVVTIKGFKDTDSLQKDHQSILDGQ
                     KTHTESIIVHLSEGFTYLKDAMPTFTVKLTEIFHSVEQISSELKEQRQQKDRQLGSLN
                     FRQETIQESLENVNRLNDEMQRVHLENEELRAQNYDLIQKKRNLEQKIVELKEENRQL
                     NYQLRRGKNRSRDLGNELEL"
     gene            6274..6606
                     /locus_tag="ACGE70_002059"
     CDS             6274..6606
                     /locus_tag="ACGE70_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693683.1"
                     /translation="MDKYIHMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWLAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
     gene            6607..7200
                     /locus_tag="ACGE70_002060"
     CDS             6607..7200
                     /locus_tag="ACGE70_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693684.1"
                     /translation="MRVKGQNSVDKKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            7222..>8867
                     /locus_tag="ACGE70_002061"
     CDS             7222..>8867
                     /locus_tag="ACGE70_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFF8693685.1"
                     /translation="MLDVIKTAFSTQSNQKVIDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLAFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECH"
BASE COUNT         3215 a         1418 c         1793 g         2441 t
ORIGIN      
        1 aaagaacaag aagaaaaaca acggcaaaaa gaaaaagagc gtgaacaatt agaaataaga
       61 aaacgggaac aggatcttgc ggaacaaaaa atggcgatag aaaaacaacg gttagagcaa
      121 gaaaaggaaa aagaagcagc tgctcgtaag gaacgtcaag aacaaatgga tacattgcgt
      181 caagcacagt tgaattatct agcacaacct caaccagtag ctgctgttca aacgccagtg
      241 aacaaccaag caaatttaac tccttcaccc cagcgttcct ttaacggatg gatggtggga
      301 tgtattgcaa gtttaagctt gcttctaggt ggaggaacag tatttgcttt taatcatatc
      361 aatcaatctt cacaggctga ttctgtagcc acaacggcgt caatcaagga agaagcccag
      421 gaacaagcaa gaaaagagta tgaacaacaa ttagaagaat tattgaatcg taccaatcag
      481 tcaacaacag agtcaagcat ggaaaatagc aaagagagtg attctagtaa ggctaaagaa
      541 ggatcaagcg attcaactgt acaatcaaac acttccgaaa gttcagaatc aaatgaggtg
      601 aaataatgcg tgttagagaa acaacgaagc gaaaagacaa acgattcaat ttagaaaaga
      661 ataggtatct ttctcatttc tatcgctata ctccctcagg agaagagcta ccagaaaaac
      721 atgacaaacg catttcgaaa aagatttttt atagtggact tgtatacctc cctttagagt
      781 atttagtaac aagagtacca gaagttttca accgattgga aattgttaag ggaagcttac
      841 aaggaataaa tctgtttcaa ttacaggaaa tgccacaggt ctttcatttt gattcagctg
      901 gtttttggaa tccatttact cctacaacta gtttgggtac acgtttagca atttcgacag
      961 tttgtgtggt tccagctgta cttatgggat tgaaaatgga catgtggttt cgtcctctag
     1021 cagatggaca aaaaggggat aatcgattaa tgaccttaaa agaaatccaa cagatttatc
     1081 cgcaaatacc tgaaacaaaa actacttttc caggttatgg aggtattcca atcacacatt
     1141 acaaaaaata ttggtacatt caaacagata ctgttaatac gtgtattgtt ggtacctctc
     1201 gatcagggaa aggtcagatt gaagtgttag ccaccattga caatttatct cgtgcagaaa
     1261 aacaaagttc aatggtcgtt aatgatccaa aaacagaatt atatgttggt agtaaagatg
     1321 aactagaagc tagagggtat gatgtatatg cctttaatat caccgatcca ctgcaatcaa
     1381 tgagtgataa tccattggca ctcattgtga aatactggaa gcgaggagac atagacaccg
     1441 caacacagtt aaccaatact tttacgaata cgatttatta tgatgcaaat gcttcagaca
     1501 ataaatactt taacgaaaat gcgcagaaag ctgtcaatgc gcttattttt gcgttattgg
     1561 aacaagcgga tcaaacaggg gattactcca aattaacacc aaataatctc gttgaactac
     1621 tttcagaaat tggcggtttt aattattatc cagatcctga gaatgaattc aagcagctca
     1681 atgccttaga tgagttcatg aatcagttac cccctggaaa tatagcaaaa aaacaatatg
     1741 gggcaacaaa aattggatct gataaagcga aagggaatat cttgtctacg gcaattacag
     1801 gattgaatcc atttacccta acgaaaattg cgaagatgac ttctcaatcg accattgatt
     1861 acaagtccat tggttttccg aagtatttgg atctcaaatt acatgaaagc ctattaaatc
     1921 aacgcattgt cgtggaattt tatcataagg gaaagaaatt tcacgaggaa cgagtcaaag
     1981 ttggctatcg tggtttttgt gaaatcaact ttgattgtca tttagagcag ggagatcatg
     2041 tttgcttaaa atacaatcat caaggaaaac aatatgttgc ttggtatgag ttttctcaaa
     2101 aagtattgaa agacgaagag ggaaaaatcg tttatcgaaa aaaacgagga gaaactcatt
     2161 tgccagaact agaaaaagaa gccgttctaa cattagatga gaagaaaagt aacctattag
     2221 tagagtctat tcggatgcac tataccgata aaccaatggc tatttttatg ttaacgcctg
     2281 attatgatcc atctaaccat atcattgtta gcatctttct tagtcagttg tataaagaat
     2341 tatctacgca atgtgttaag actaaaggag ataaatgtca ccgtaggatt cattttattt
     2401 tagatgaatt tggaaatatg ccaccgatgg acaacatgga aggaatcatg acggttacgg
     2461 caggtcgtaa tatgttatgg gatctgttta ttcaatctta tcagcaactt tatgcgaagt
     2521 atggagaaca aaacgcagcg attatcaaag aaaattgtca gacacatatt tatattatgt
     2581 cgacagatga taataccatc gaagaatttt ctaaaaaagt ggggaataag acggttgaac
     2641 aagagaacag taccatcaat tcattaggta tgaatactca tatcaatcgc aatgttgatt
     2701 cagatcgtat cttaactcct gaaagaattt ctactttttt agaaggtgaa acgattgtac
     2761 tcgctccttt aaaacgacaa gatttgaaag gtcataaaat acggccgtat cctattttta
     2821 atcatgggga aacgaatctt ccttatgctt atcaatatat tgaagatttc aatccatcgt
     2881 gggatatcaa tgatatagac atacattgta cacatgcgga cttggatctt aaggctttac
     2941 aaattgatta tgattatttc ctaccagatg aatatgctag aaatctctat catggactgc
     3001 atgatcaatt agaagaaaaa aacgaagaaa atatgtttta taaaatcgtt caatcttttg
     3061 agaatgagac ggctaaaaaa ttgttaattc aattttacga agaacaagca acggatcgtc
     3121 ttcaagaata tgttgaaaag ttgatcaaaa atgaagaaat ttctgaaatg attaggaaac
     3181 ggctgttatt ggaagctgaa aaggcggtga aagaatgatc attggaggaa ttttctctgg
     3241 catagccgac tggtggtcag gtttattcgg agaaggggat gcagatccca aaaccgtaac
     3301 gaaagtctta caagattatg gtaattatct gcagtatcaa gatgtattca gttggatttg
     3361 taatactttt ttatggggat tgatcaaagg gttatataat ctcaatactt tattagaaaa
     3421 aacaatttat caaagctttg atttaaaaga cttattgaac gccgctggtg ttaatgaact
     3481 atttcaaagt ttgattagta aggtattggc tcttttttgt gtcgtcacat taatttattt
     3541 aggcttgaaa ttttttatga gtaaacaccc accgaaagtc aaaaatatct tggttaattt
     3601 atttatggcc atgatcttga tttttggtgg tagttcttta atcgatgaag ggttaaatat
     3661 cagtaaaaac ttttatggag acgtcactgc agcaaataaa gcagataaaa cgggttcacc
     3721 tgcttttcaa attattcaaa ataatgtgat tgatgtcagt acattactag cgactgaccc
     3781 gaataaagta gaatccttac caacagataa aagaaatgct ttaacagcga aaaatttcaa
     3841 aacagcgaat atcaatggga ttattacacc tgatcaagct aagaaaatgt ctgaatcaag
     3901 tgatattagt acagtacaaa aagatcggtt aaaggcatta cagtatcgac tagagttaga
     3961 cgataatggg aaagaaatcc ctgttcaaat tagtgatgaa ggtttagcaa aatatgtgta
     4021 tacgtcaggc tatcgtcgat acattagtag tccagggatt attttggctg gggaatcttc
     4081 tttgtcggta gcctatttat ttattttgtt tacaatcgtt acttgcatta ttgagctagt
     4141 atttaaaaaa ttctatttag taattgccgc gtcaacggac tttgagacag gacaacggat
     4201 gaaaagtgcg atagaagata tttctcaatc cttcttgtta ttggcgttta cgattttaga
     4261 attacgaatc tatacattaa tgctttcagg tatcggtgat ttgcatgcgg taggaaagat
     4321 caatggattc ttgtatgttg ttgctttaat tgtgctaact attgcgctat tcaaaggctc
     4381 tcaatcggtt acgaagattt tcggtgttga tacgagtctg aaaaacggca gcaattctct
     4441 tcttagcatg tttgccttag gaaatattgc gaaaaatgtg ggtagcggag ctaaaaacct
     4501 tgcaagtgct ggtaagagtg gtttaagcca tttaaatgat attcgtaaga atggatttgg
     4561 ccatcgaaaa gatattaccc atgataacgg ggaagaacaa tcagacgata aagctacaac
     4621 tgtacctaat agtaagaaga cagtttccga aatgttcggg aaagcgaaaa atggtgtttc
     4681 taaagccgca gaaggtcttg gttacgtcaa tgaacgtggg ttaaaaggaa cagttgaaga
     4741 tcttacagaa aaagccaaag acaccttaga agagaaatta gacggcacga gagctaaaga
     4801 atttgctgaa gctgtgaaga atccatccga agcactagaa aaacaaaaag aaaaagcttc
     4861 agcagtcaaa gaaggactaa aagatgcgta tggtgaagga caagtcaatg cagcagtgaa
     4921 ggcaaataca acaagtgaaa aagaaaatca agaaggtgca gcgattcctt cagccaaaga
     4981 tcaccaagat gagttacatt ctaacgaaaa ttcgatcgga gaaaaagatt cagaagaagg
     5041 acaacatggt cagttaccaa aaaccgatcc agaacaatat cgttataaat ctaacattaa
     5101 ttctgaaaac gaaaaacgca tttcagattt accaaaacga aatcaagaag tacaaacgga
     5161 tgaggattcc atcgatctgc caaatgataa tggcatcgaa gttggaactg aaaaatcaac
     5221 gaaaattggt ttgcctaaga cacaaaattt accaatgaaa gatagtcgtt tgaatcaatt
     5281 caaagagaat gatcaaagct ttgttcatca atcacctgca ggtactgttc aaatgaaatc
     5341 aagtcgtttg cctgaaaatg gaaatctgac aactgtttct tacaatttag ctgaaagtgg
     5401 tgaaacagag aatgcgtccg ttcaaaatat tgtgacaaaa gaagctgcac ctaagccaat
     5461 caataaagtt cataatggat acagaccagc aaacgtagca tttactgcac cgacaaaaat
     5521 aaatgtgaca agagaaacta aaaacaggaa caatgaattg aaagaacaac tattgaagga
     5581 tatcaaacca taaaaggcct atttaagggc ctttttcttt ataaaacaag tttaaaatgg
     5641 aaaggagaaa attgtggagg atattttaat agctacgttt ataactgcac tgggagcatt
     5701 tttaacgata tttgtggtga ctattaaagg atttaaagat acggatagtt tacaaaaaga
     5761 tcatcagtct attttagatg gtcaaaaaac acataccgaa agcattattg tacacctttc
     5821 agaaggattc acttatttga aagatgctat gcccacattc acagtaaaat taacggaaat
     5881 atttcattct gttgagcaaa tatcgtcaga attgaaagag caaagacaac aaaaagatcg
     5941 tcaacttggc tcacttaatt ttaggcaaga aacgatacaa gaatcattgg agaatgtaaa
     6001 tcgattaaac gatgaaatgc aacgagttca tttggaaaat gaagaattac gcgcacaaaa
     6061 ttatgattta attcaaaaga aaagaaatct ggaacaaaaa attgttgaat taaaagaaga
     6121 aaatcgtcaa ttgaattatc aattacgacg aggaaaaaat cgttctcgtg atttaggaaa
     6181 tgaattagag ttgtaatatt tagaaaaacg ctgtgagaaa tcacagtgtt ttttatttct
     6241 aaaaatacaa aatataaggg gtatggttat attatggata aatacattca tatgaaaaat
     6301 cctccaagat ttagtgggga atggaagttt ttcgcctttt tctttttaga tctattaacg
     6361 cttggtggct gctttttttg ggcgaaaata gtcaataatc ttgtccactt accactcttt
     6421 tttgcactct tgaattatgg attatggctt gcgatagcgt tagtgattgt ttggcgaccc
     6481 tataataacc ctggtcaacg ccaaattttt gcgattatcg aatctatttt ctttacggat
     6541 aagaaccact atcatgcgtt agatcctaac cgaaattatc atggtacgta taaggaggat
     6601 gaataaatgc gagtcaaagg acaaaattca gttgataaga aatccatttt gcagtttgga
     6661 aaaccagaag agcgctctat cttggactta tttccatata aatcctgtac agaagaggga
     6721 gtcttaatta ctagagaaga tcattttcag cgttattatc gtgtcgtttc cactgacgtg
     6781 gagggattaa atgaacaaga gaaactagaa cgaatgaatc agttgaccac cgtcatgcgt
     6841 acgtatgtgc caacaattaa gttagttagt ttgaccacag agacggatct aacggaacaa
     6901 atcgtgcaaa agcggcagtt attagataaa aatcgtatgg aacagtcact aggaagaaat
     6961 ctacagcgat taagaaaata tgaaaagaaa ttagtagagg agatccagga attaaaacgg
     7021 gcagaacaag aacgtcctga cttaagcttc tttttcgtta ttgaaggaaa gacaatcaag
     7081 gatttacaaa ctagaaatcg tcagttaatg cgatctagtg gcattttagg gttaaaacca
     7141 ctctacaaaa aagaattgat taagattctt tatcgaatga acaacatgaa tgacgaataa
     7201 aaaagatgga ggaaatgatt catgctagat gtgataaaaa cagctttttc aacgcaaagt
     7261 aatcaaaagg tgattgatcc attaaaacaa cttggtaatc gtgaaataga gattttaaaa
     7321 tcaaaaggtt atgatttaga tttcttaaaa caagtgatgc cacaaggtgg gttgaatttt
     7381 gatgaagaat atgtgaccta tggaaatggt tattcacgat gtgtgacagt ttatcattac
     7441 acaaaaaatc ctactctttt ttggttggcc aatctcatga acaatcctca tacagtggcc
     7501 acactagatg ttagcacaga tgaaaagcaa aaagtgattg atcaaatcaa ccgcgcatta
     7561 gatgaattaa atgatcgatc agataatgaa cgaaaagcga ctgaccgaaa taaaagtgcg
     7621 gaagaatacc tggaactagc ggaatatgcc cgaaaattga cttctaatgg ggaaatttct
     7681 aaacaagtca aagtgagagt acatgtttac ggaaatacga tccaagaagt ggatgaacgg
     7741 gcgcaggaac tgatcgaaaa tctaaaaggg atggattatc gtgcggtgac ttatctgcat
     7801 aaaaccaaac gagattggca atcgatgttt accatgttcg atgaacaagc tgaatggttt
     7861 ggaaatatca aagggcaatc acttccatcg ttaaacattg gcggaggatt cccgttccac
     7921 caccaaacgc taaaagatcc aggaggcaca caatatgggg aaaccatgac aggcggccca
     7981 tttgtctgga atgccacgta taaaaatagt gtacgtttat ctaccaatac cttgcttcta
     8041 ggaatgatgg gagctggtaa atcgaccgtt cttaaaatga tcgcggaagc ccatttagct
     8101 gcaggagact ttatttgggg ctttgaaaag ggaaaagact ttattccttt cttaaaggaa
     8161 tataacggta ttatggtgcg tttagacgga tctgacggaa tgatcaatcc tttagagata
     8221 tttgccacac ggacgtatga tgaagcatcc agtttatatg acgatggatc agtcaaagat
     8281 ttgaagatca atgaagcagc gtcctatcaa acacacttag acaaagtggt ctatcaggta
     8341 cagttagtga gtcctcagct aaaaggaacg atgaaagcag aatttaagac ctacttaaat
     8401 cgtttttatg aagagtatgg aacagtacca cgcggcttta cttcctcaaa ttcgcgttca
     8461 aatactgata cgcaagtcac agggaaagat ccagaagctt atccgacgtt caaagaattt
     8521 ttagcctttt taggacaact ggaactacca ggggcttccc aagagaaaaa gaatcggaaa
     8581 gaagaaatgg agtctatcgt ggaatcctta tgtgagactt atggcatgat ttttgatggt
     8641 catagtacga ttcgacacct tgaccaacaa cagttggtct tttttgacat tgactctatt
     8701 acaggcctag atcgaggggt acaacaatgt cagatgtaca tggccatgac gttgatttgg
     8761 aatcaggcat tgattcaagg acgaagacaa cgacgcttaa ttaaagaaaa acaaatggaa
     8821 gaacaagact tcaaacgctt cttggcgatg attgatgagt gccataa
//
LOCUS       DAXWOO010000114         8864 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.115, whole genome shotgun sequence.
ACCESSION   DAXWOO010000114 DAXWOO010000000
VERSION     DAXWOO010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.115"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(347..2728)
                     /locus_tag="ACGE70_002062"
     CDS             complement(347..2728)
                     /locus_tag="ACGE70_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFF8693686.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITERTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2718..3365)
                     /gene="recU"
                     /locus_tag="ACGE70_002063"
     CDS             complement(2718..3365)
                     /gene="recU"
                     /locus_tag="ACGE70_002063"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFF8693687.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3429..3968
                     /locus_tag="ACGE70_002064"
     CDS             3429..3968
                     /locus_tag="ACGE70_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFF8693688.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            4058..4477
                     /gene="gpsB"
                     /locus_tag="ACGE70_002065"
     CDS             4058..4477
                     /gene="gpsB"
                     /locus_tag="ACGE70_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFF8693689.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4499..4866
                     /gene="rnpB"
                     /locus_tag="ACGE70_002066"
     ncRNA           4499..4866
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACGE70_002066"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            5075..6241
                     /locus_tag="ACGE70_002067"
     CDS             5075..6241
                     /locus_tag="ACGE70_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFF8693690.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6287..7783
                     /locus_tag="ACGE70_002068"
     CDS             6287..7783
                     /locus_tag="ACGE70_002068"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFF8693691.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7864..8547
                     /locus_tag="ACGE70_002069"
     CDS             7864..8547
                     /locus_tag="ACGE70_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFF8693692.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
BASE COUNT         2807 a         1595 c         1868 g         2594 t
ORIGIN      
        1 atcagataac tcaaaatgaa gccttctttc aaaaaattat tcaaaaccat atattgtatt
       61 ttttatttga taataacaat gggattatta agaagtggca ctaaataaaa gactagtgtg
      121 tatttagccg atttaattat aaacaagtaa atggataaag tatatgtaat gataaaagaa
      181 aaatggattt tctaattttt aagctaaatg ttagatcaca catgtatatg ctggatagtc
      241 ttataaaaaa tcatgtaata cagcttggta catgattttt atttgtagct gttttttaca
      301 tagacacaaa aaagaaacca cgcttgtaga cgtggtttct tttttactat ctgtttccag
      361 aagctgaggt gctgctggat ggtgtatggt catttgcagc atccgctccg ctagttgctg
      421 gctcactgga agaagcagga ggagcagatg aagacggtgt cgagctttct tcggatgaag
      481 aagaaggaac cgttgttgaa gacgctggct cgcttgtttc tgctgtcgtg ctttcttttg
      541 aggactcagc agttgattcg ctttgactag tagagctaga gctttgagtc gttgtctctg
      601 ttgttgagct tccaggagtc tggacatagc tggaagatgg aatagttgtt gatggtgtaa
      661 ttgcattcga tctagctgta tattgatctt tgtagtacag ttcaccgccc acacgaatta
      721 atccgcttgg catctcccag tctgtatttg tcacgttagc tgaaacatac tgcataagct
      781 cgcgataaac atcagaggct acgtgtgacg attctgaagt gacaggtgtc atttttttat
      841 tgtaacctgt ccacacggaa atcgaataat ttggcgtata gcccgcaaat aggatatctg
      901 gatacacccc agaagaaata ccaagttttg catactcgtc atctgtgtaa ttggatgttc
      961 cggtcttacc ggcttgatat aacccagcga tttgcgcatt cgtaccagtt ctttcagtga
     1021 tcgtatcttt taaaatgtct gtgatcatat aagctgtttc tggagacatg gctgtttttc
     1081 catctggctc ataagtttct tctgttccgt cttggaagac gattttatta acatattgag
     1141 gtttgtaata agtccctcca ttggcgaaag ccgcataggc tgctgccatt tttaaggaag
     1201 aagctccgta ttttgtccca tcttgttctt ccgtattact agagatcgca ttcgattgat
     1261 ggatcgtact gtattcgatc ccaagatttt ttaagaaaga agccacttta tctgaaccga
     1321 cttcattgaa taatttgact gctggaacat tacgggataa gtaaagtgct tggcgtaaag
     1381 taatggtgcc catatattga ttatcccagt ttcctacagg agtcgaagtc ccttcatagt
     1441 tatacggtgc gtcagtaata gtttttccag tagaatattt caaatattca aaagctggtc
     1501 cgtaatctgt gacaggtttc atcgtagaac caaaatcacg ggaagtattc actgctaagt
     1561 tgttgcctaa agttacgtct tctgcgatgt gacgtccgcc aatctgggct tttactttcc
     1621 cggtattggt gtcgataagg gtggaggcaa cttgcatctc gtcgtctggg taagagacat
     1681 actgatccgt gttgacgata tcatatagtt tcttttgggc gtccagatcc aaattcgtat
     1741 aaatctctaa accgtctgtg tagacattct tatctgtttt ttcttgtact tcattgatga
     1801 cttctttgac ataattatcg acgatcttag tattgtcatc ggattgagtt agttcttgca
     1861 gaccatccgt taccggtaca ttaacagctt gatcatattc tgtttgagag attttttcgt
     1921 tttgaagcat cgtatacaaa acagtatctc gtcttttctt cgcctgatct ggataaacat
     1981 aaggatcata ggcggaaggg gcttgaggca tcccagcaag caatgctgtt tgcggtaaag
     2041 aaagttctga taatttctta ccaaagtaca tctcagaagc tgtctccatc ccgtacaagc
     2101 cgtttgacat atacacttta tttacgtagt aggtcaagat ctcttgtttc gatttttttt
     2161 gttccaatct tacagccatc cacgcttctt gggccttgcg tttcagagtc tgatcctcgg
     2221 cactggtgga gaagaaggaa agtttgatca attgctgtgt caaagtactt ccgccttgca
     2281 gtccgccaga ggtaaagtta gagagggcag aaccgatgat ccgaataggg tcaaccccga
     2341 tgtgtttata aaaacggcga tcttctaccg acacgatcgc atcttctaat gttttcggta
     2401 attcattcgc agaaattttt tcacgctttt ctgcgcctaa atcttcgaat agttcgccat
     2461 cttgtgtata tagtttggaa gatacggtag cgtctaattt tttatccgtc aattcgggcg
     2521 catcttttgc gtaataccag aaaagtccta cacctgctaa aaagaggata cagaaaaatg
     2581 agagtattcc taaaaggatc ttgataaata atcctttttt atgtgttcca gaggactttc
     2641 cagaaccttt gccagaattc ttcttcacac tctttttagg agtaggctgt ttctgtcgtc
     2701 tagagcttct agtttgttca tttgccattc ttgctttctc cttcaataga taatgttgat
     2761 aaatagaagt caaccgcgtc caaataagga atccgcggag aaataccagt tttgatctct
     2821 attcctagtt cttcgatcaa agataaaggt agtgatttgc gtcctttttc cttttgggcg
     2881 tcccagtaag caatcaaatg aacactattc aaataaaaac agcgcttcaa agacgaaaac
     2941 cataacaaga caaagcagac cccattttgt tcgaggcatg ctttcatatg ttcgatttga
     3001 tgggcatgga agtttttaaa gggaaaagat gttttgttct tggtttcttt tgcttcaaag
     3061 tccaagtaaa agcctcgata gacgccatta tagtctgtag ttgaggcttg ggtaaagtag
     3121 gcttccttga tcacagcagc acttctctta ggatagtcta ctttcacgat ttggataggg
     3181 gtaggctttt tatggataac cgccaacccg cgggaaagat aatatttatt actttcattg
     3241 attgcttctt caaagcgcat cccccgattg ccgaattcag tgatctgatt tcttttttct
     3301 tgaatttttt cgacgggaga ggcattctta taataaggtt ggccatttgg atagcgcaat
     3361 accatcttta atcacactcc taggccacat tatagcaaaa aagcggatag aaagaaaagg
     3421 aatctgagat ggataagtca aaagtaatct atgtgtctgg gtatcgaagt ttcgaactcg
     3481 gtgtttttaa agaaaatgat cctaaaatcc aagttgttaa aaatgtttta aaaaaagaac
     3541 tccatcagtt agcagaagaa gggctggaat gggtacttgt cagtgggaat ttgggcgtcg
     3601 aactatgggc tgtgcaaata gtagcggaat tgaaagaaga atatccggaa ctatctcttg
     3661 gtattttgta tcctttttct gattttggta ataactggaa tgaagggaac caagaaaaaa
     3721 aacaattagc agaacaatta gcagattatg tcgaagctgt tagccatcaa ccctaccaat
     3781 cacctagtca gctaaagaat cacactcgtt ttatacttga gcatacagtg ggctgtctgc
     3841 tgatctatga tgaagaattc cctggaaaaa cgaagtattt ttttgaagat gcacagaatt
     3901 accaagcaga tcatccctac gaaattcggc tgatcagtat ggacgatctt caaaatttta
     3961 gtgaataatc gatattgtgt ttgatttatc aacttttttc ctatacaatg aaaaaggtag
     4021 aaatgttatt acgtaaatga aaactagagg tgtaaatatg gcgaatttgg tttatagtcc
     4081 taaagacatt ttacaacaag aattcaaaac aaaaatgcgc ggctatgatc ccgttgaagt
     4141 tgatgaattc ttagacaata tcatcaaaga ttatgaaact tacagcaaag aactccttgc
     4201 tttacaagaa gaaaacgatc gattgagtgc aaaagtagct caattatcta aaactcaagg
     4261 agcagctcaa acacgcgtgc aacaaacaga ggcaccaaag agcgcagcag tgaccaattt
     4321 tgatatctta aaacgcttgt caaatcttga acgtgaagta tttggtaaaa agcttgatca
     4381 acaagcaagt gcagtcaaac cagcacaacc taatccaaat aactacacaa atgcagacac
     4441 aagcttagat gataatgaaa aaacacgtca attttaattt ttgattaatg aatttgcggt
     4501 tttcgggtaa tcgcggtctg acttgatcag gctgaggaaa gtccatgctc gcacaggctg
     4561 tgatgcctgt agtgttcgtg cttagcgaaa ccataagcta aggtactctt tggagtaacg
     4621 gcaggaaaac aagctaaggc ttcggctatg cttaagtatc cttgaaagtg ccacagtgac
     4681 gaagcggctg gggaaaccca tccggtggaa cgcggtaaac ccctcgagcg agcaacccaa
     4741 acaatggtag gggcgctttt tgtctggaag aagaacagga caaaaaggca gtataactgc
     4801 agatagatgg ttaccgcttt gttttttctc cctggaaaag caaagtacag aacatggctt
     4861 atagaaaacc gcaaacatca gggaattagg tcgtgacaaa agtaatgcgt tataagaaac
     4921 aagaaaaaca tttgaaagct atttttcaat acttcagatg aatcgttctt attacaaaac
     4981 aaacttttgt atcaccggtt attcggaatt tgagtaaaag tgtggggtag gactgatgtc
     5041 ccacctcctt tttttataaa taaggagatt gtgtatggaa aaaaacagga agttcaacct
     5101 cgtcgcaact gcggcaagcg ggctagaagc actggttggt aaagaattaa gagatcttgg
     5161 aatcgaatgc caagttgaga acggacgtgc acgttttgaa ggaacaatgg aaacgatcgc
     5221 tacagctaat ttatggttac gtacagcaga tcgagtaaaa atcgtggttg gggaattcga
     5281 tgcgtatacg tttgatgaat tgtttgaaaa cgtaaaagca ttgccttggg aagattttct
     5341 tccactagat gcagcttttc ctgtagctgg aaaatcgatc aagtcgaaac tatatagcac
     5401 acctgattgc caagcgatca caaaaaaagc aatcgttgaa cgtctccgta cctattatca
     5461 ccgtccagct tctgtaccac tgacggaaac tggcgcacat tttcagttgg aagtagcttt
     5521 gctgaaagac catgtgatgg taactctgga tacgacagga ccaagtttat tcaaacgtgg
     5581 ttatcgctta gaaaaaggtg gagcaccatt gaaagaaaac atggcagcag cacttattat
     5641 gttgacaaat tggcgaaaag accgtccatt ttatgatcct gtatgtggtt caggtacgct
     5701 ttgtattgaa gcagccttaa taggccataa tatcgcacca ggattcaatc gtgagtttgc
     5761 ttgcgaatct tgggattggt tcagtcaaga aatcatggaa aatgttcgat cagcagccga
     5821 agaaaaagca gattatgata ttgaattaga tattactggt tcagatatca atggacggat
     5881 gattgaaatt gccaaagcca atgcagaaga aatcggatta ggctcttcga ttacctttaa
     5941 gcaacaagca gtcaaagact tcaaaacgga taaagaatac ggggtcatcg tggctaatcc
     6001 gccttacgga gaacgtctag gagaagaaga aactgttcgg aaattatata aagaaatggg
     6061 cgatgtattc cgtccattaa aaacatggag taaatatatc ttgactagcg acttagcgtt
     6121 tgaggaattt tacggacaaa aagcaaccaa aaaaagaaaa ttgtacaatg gtgcattgcg
     6181 tacggatcta ttccagtatt ggggaactcg tccgcctaga aaaaaagaga aagacgaata
     6241 atcttactaa ttgattcatt caattggatg tttggaggaa tagggaatga aagaaaaaga
     6301 gtttttacaa gaagtaaaag aaatcaatct tctacaacaa gctttaggaa tcctcgattg
     6361 ggatacacaa acaggtatgc ctgaaaaagc tagtgcatac cgaagtgaag tagacagcta
     6421 tctttacagc atttattttt caaaaaaaat cggtccaaaa atcaaagaag ccattcgcta
     6481 tttttctgaa catccggaag aattgtcgga agttggaact gccgtttttg ccaaagtaaa
     6541 agaagagtat gatttggaac atcgcgtacc ggaagatctt atgagaaaat atacagctgc
     6601 cgtttcttca gcacacggac aatggcaaaa agctagagaa acacaacaat tttctgattt
     6661 tcaacacgca ttgaatcaaa atattgaatt aacaaaacag ttgatacctt actggagaaa
     6721 agatgaaaag acgaattatg atgttttgct gaatcaatac gaacccggga tgactgtcga
     6781 aatccttgat aaggtatttg atcagcttaa agaaggaatt ctttcgatcc gccgtacgct
     6841 ccaagaaaaa gggactgaac ctgaaacaga ctttttaaat cggagaatga cgaaaaaaca
     6901 acagaagaga tttgtagtga aagtaatcga acaacttggt tatgatttta gtcgtggtcg
     6961 tttagatgac actgtccacc catttatgac tggaattaat ccgaatgatg cacgaatcac
     7021 gacacgatgg aacgaaacag atttcaaaat ggctgttttt ggagtgatcc acgaggctgg
     7081 tcatggtatg tatgaacaag acatcgatga taaatatgcg tatacaccgg tttatgaagg
     7141 tacatcaatg ggtatccatg aatcacaatc gttgtttaac gagatcatta ttggaagtag
     7201 tcgcagcttc tggaaaaagc aatatccttt ctttcaagaa tgtgcagaag gaacattcga
     7261 tgatattcct tttgacaaat tttatcgttc attgaaccat acaaaatcaa gtttggttcg
     7321 tatcgaggca gacagtttga cttaccctct ccatatcatc attcgttatg aaatcgaaaa
     7381 aatgattttc aatgaagggg tagatgttgc tgaactacct gaaatatgga atcaaaaata
     7441 tgaagagtat ctgggaatac gaccaacaaa tgatgtggaa ggcatcctac aagacgttca
     7501 ttggtcaggt gcaagctttg gttattttcc ctcttatgca ttagggtaca tgtatgcggc
     7561 acaattactt cattctttgc agcaagaaat agatgtggaa aatgttctag cgtcagacga
     7621 ttattctcca atcaaaaact ggatgtcaga gcatatccat caatatggtg catctagaaa
     7681 accaaatcaa ttaatcatgg atgcaacaca tgaaccgctt aatccgcaat acctgatcga
     7741 ctatatgaaa gacctgtatt ttaatgtata tcaggtaaaa taaaatacaa tttacaaaga
     7801 tagctgaaat tcatctaatc tatagatgtt ttcagctatt tacttaagaa agaagtggaa
     7861 cgaatggata aattacaagt gatcgctgat tatagtatgg accaattagc gcatgatcaa
     7921 acaggacatg ggagcgatca taccaaaaga gttgtcaagt tagctgaaaa aattttggag
     7981 acggaaccgc aggcagaccg ctttgtgacg cttgcagcag cttacctaca tgatacgatt
     8041 gatgataaag tcgttaaaaa tgagaacgag gcaaagcaac agcttcgtgt attccttcgc
     8101 tctttgccaa tagtagagga acagatctcg atgatttttt ccattatcga aaatatgtcc
     8161 ttttctaaaa atctctctga agcggccgaa ctatctctgg aaggtaagat cgttcaagac
     8221 gctgatcgta tcgaagcatt aggagcaatc ggcattttgc gtactgctta tttcggcggt
     8281 ggacatgggc acccgatttt tgatcctgaa ctgtatccgc aaacattcaa ggacaaagaa
     8341 aattaccgaa aaggcacgac agtgatcaat catttttatg aaaaactgtt cttgctgtct
     8401 gataaaatga atactgatta cggatatgaa gaagccaaac gaagagaaac cttcatgcgt
     8461 gaattcttag aagaattttt tctggaatgg tctattgacg atgaaaattt tactccagac
     8521 aattggatca aaaataacaa aaaatgatgg aggttgtgac agaagtggtc agctccaaga
     8581 tatttcggat cattttccgt aaaagttact tctggaacac tgtttaccaa gaggtcgtga
     8641 gcctgccgtt cagctccgag tcacaaggaa caatttcaaa aaatagcttc tcgtgtttga
     8701 tgaatattgt aacttgtgat tcggaattgg caggagaaca ccgtttattc ggaagaatag
     8761 gactgtgaca agacttttgt cacagtcctt ttttcgaatt caacaagtaa aacttttaag
     8821 aaatattcag agagtaaaat taaaaattgc tgataaacga agat
//
LOCUS       DAXWOO010000115         8774 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.116, whole genome shotgun sequence.
ACCESSION   DAXWOO010000115 DAXWOO010000000
VERSION     DAXWOO010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.116"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(476..1063)
                     /locus_tag="ACGE70_002070"
     CDS             complement(476..1063)
                     /locus_tag="ACGE70_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693693.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            1238..2560
                     /locus_tag="ACGE70_002071"
     CDS             1238..2560
                     /locus_tag="ACGE70_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFF8693694.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            2715..3491
                     /locus_tag="ACGE70_002072"
     CDS             2715..3491
                     /locus_tag="ACGE70_002072"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFF8693695.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            3484..4053
                     /gene="rnmV"
                     /locus_tag="ACGE70_002073"
     CDS             3484..4053
                     /gene="rnmV"
                     /locus_tag="ACGE70_002073"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFF8693696.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            4046..4930
                     /gene="rsmA"
                     /locus_tag="ACGE70_002074"
     CDS             4046..4930
                     /gene="rsmA"
                     /locus_tag="ACGE70_002074"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFF8693697.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            5381..6010
                     /locus_tag="ACGE70_002075"
     CDS             5381..6010
                     /locus_tag="ACGE70_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693698.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(6134..7243)
                     /locus_tag="ACGE70_002076"
     CDS             complement(6134..7243)
                     /locus_tag="ACGE70_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8693699.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            7452..7874
                     /gene="mgsA"
                     /locus_tag="ACGE70_002077"
     CDS             7452..7874
                     /gene="mgsA"
                     /locus_tag="ACGE70_002077"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFF8693700.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      8061..8159
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            8197..8733
                     /locus_tag="ACGE70_002078"
     CDS             8197..8733
                     /locus_tag="ACGE70_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFF8693701.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
BASE COUNT         2774 a         1554 c         1796 g         2650 t
ORIGIN      
        1 ttcaaagggg gtatgacact agttgtgtca tacccccttt ttccgaaatt tgagaagcaa
       61 ttttcgtaaa ttccttctta ttacttgaag ccaatcatat aatacagaaa cagagaacta
      121 tccaaaataa atcctagaaa aagaagtacg agcttcgctg atttttgtta atttagaata
      181 atactaatag gaatcgtaaa aacagaaaaa aagcataatg ataaagggtc gggcagattt
      241 taattgattc atctggtact cttttttatt gtccttacat tcataaagtt tgaatgcaat
      301 gactttatta catctatatt tatatctaac agtagcgttg atgtgttaat tataagttct
      361 atcactttat gttcaattag tttgtgctct tatatgtgtg attgaaaata tagataaaaa
      421 aacaatcatc ggctttgact tcgatgattg ttttctttat tttgtaaaaa tcctatcact
      481 tgattttatg gtaacctttt atttttgtct tcaaacggct tggtaagaga gcagtaagca
      541 gtgcaaatgg gatcgaaaag acataccaga aaatcgtacg tccgatacca taatgttgat
      601 agagctttgt caacatcacg tgtaacaaga tcaaccctaa aagcaaggag ccagctataa
      661 accaatgatt atcccaatgc atgacttcac taagtgccat cactccaaaa ggatagaaat
      721 aaaaatagat cccgactaag gtcatccgcc agttctttgt aaatgttccg atattcaata
      781 aaaagaaaat cgctgaaagc aaaataccga taggcggaaa aagcccaacc aaaaaggaat
      841 acaacatccc ccacaagatc atcacaggac ctttgatcaa tgcaacgatt gcgatgaaca
      901 aagtcagcaa tattagtttt tgtgataacg ttgcaacagt cattaatcca aataaaatta
      961 atgtgaacca atgccaatgg gactgctgtt gttctttacg ggaaaaatcg acccggttgg
     1021 ataaataatc tgctaatttc ggataagaac gcttaaaatc cataaactgt tcctccttcg
     1081 tggaaaaggt aggtttagta tacgaaaaat aaaaaagaaa tacatccgtc ttaagaggga
     1141 gtttttcact gaaagtggag aaaaaagaat ggtaaattaa aatattctca gttatactga
     1201 aaattatacc aaatttcaaa gaagaaagag gatgggaatg aaaggcaaac taaaacgaga
     1261 aatcaatctg tttggcgcat tagctaccgt tatgggaacc gtgatcggtg caggcgtctt
     1321 cttcaaaact gctgctgtta ctgctagtac tcagtctaca agtttgacat tgctagcttg
     1381 gcttttgggt ggtttcttaa cgatctgtgc tggattgaca gtggctgaat tagctacagc
     1441 aatcccggaa actggtggag ctgtaaaata tattgaagct gcgtatggaa aattgcccag
     1501 ctttttatta ggatgggcgc aaagcttgat ttactttcct gctaatattg ctgcattatc
     1561 gattatcttt gcgacacaat tgacgaactt attacaatta tctaccgatt atcttttgtt
     1621 gattgcagtg atcaccgctg tttctgtgac agggttgaat ttgttaggga caaaagtcgg
     1681 tgcttctgta caatcagtga cgttgattgt taaattaata ccaattgctg tgattgtcat
     1741 atggggatta ttgaccccag gacaaggaac tgtccagtta tttccaatcg aagctggaaa
     1801 agatgttact tttgtcgaag gattgagcag tgcgttgtta gcaacgttgt ttgcttatga
     1861 cggctggtta ggtgtcggtg caatggcagg ggaaatgaaa cggccggaaa aagatttgcc
     1921 taaagcaatt attcttggtt tgagttttgt aacagtcgtt tatctattga tcaattttgt
     1981 ttttttgaaa actttgccga ttgatcatct tgccggtaat ttgaatgcag cttctgaagc
     2041 atcagacgtc atttttggag gaatcggtgg aaagctggtc actattggga tcttgatctc
     2101 ggtatacggt gcattgaacg gttatacact aactgggatc cgcgtgcctt atgcgatggc
     2161 tttagaagac gagctacctt tcagtaaaca gctgacaaag ctttcaaaga agtttacggt
     2221 tccttatgtt cctgcggttt tccagttagc agtagcttgt attatgatga gccttggttc
     2281 tttcgacttt ttaacagaca tgttgatttt tgtgatgtgg ctattcagtt tactgatttt
     2341 tatcggtgtc tttattttaa gaaaaaaagc gccggaattg ccacgtccat ataaagtacc
     2401 actttatcca atcgttccga ttattgcgat tctcggtgct attttcattt taggtatgac
     2461 gatgatgacg caaacgaaat tagcattgat cggtataggt gttaccttga taggtatccc
     2521 agtctattat caaaaaaaga aaaaacgctc agaagagtaa aatatttcaa caacagacag
     2581 aaaaagtacg aactaggtcc tctagatctt cgtgcttttt ctatttcaat aaaacaagtt
     2641 cttctatttt gatttttgct acttctgcaa gtagtgctta tgtgctaaag taagaatgaa
     2701 aaggagagat aaacatgata tttgattctc atacccattt aaatgctgaa cagttcaatg
     2761 aagatattcc agaaacgatt cagcgagcac aagaaatggg ggtaacgaaa atggcggtcg
     2821 tgggcttcga tacgccaacg attgaaaagt ctttgcaatt aagccatgac tatccaaata
     2881 tatacagtat tatcggctgg catccgacag aggctggcag ttatacgaaa gaaatcgaaa
     2941 ataaattaca agaacaattg actttaccaa aggttgtagc attaggggaa attggcttgg
     3001 attatcattg gatggaagac ccgaaagaag tccaagataa agtatttcgc agacagattg
     3061 ctatcgcaaa agaaatgaac ctaccaatca gtattcatac tagagaagca cttgaagaca
     3121 cgtatcaaat cctaaaagaa gaagatgttc gagatatcgg tggaatcatg catagtttca
     3181 gtggtgatta tgaatgggca aaacgatttc ttgacttagg gatgcatatt tcatttagcg
     3241 gtgtcgtcac tttcaaaaaa gcattagacg tccaagaggc tgcagcaaat gtaccaatcg
     3301 aacgattact tgtggaaaca gatgcccctt atctggcacc agttcctttt agaggaaagc
     3361 gaaatgaacc aggctatact cgttatacag tagaaaagat tgctgaactt cggaatcttc
     3421 cgacagaaga agtggctgcc caaacatgga caaatgcgca ccgattattt ggtctaagcg
     3481 aacatggata agatgaagat ccaagagatt atagtcgttg aaggaaaaga cgatacaaga
     3541 aggctgcggg aagtagttga tgcagatacg atagaaacaa tagggtctgc aatcaatgaa
     3601 gagatcctga tgcaaattga acatgcccaa gagactcgtg gagtgatcgt ttttaccgat
     3661 cctgattttt caggagaaaa aataagaaaa atcattatgg aagctgttcc tcaggcaaaa
     3721 catgcttttt tgccgcgcac acaagctaaa ccaaagaaaa agggaagcct tggtgtcgag
     3781 catgcaagtg atgctgctat tttagaagca ttgaaaaagg tcgtgacgcc agacatttcc
     3841 caaacggctg tttctgaaat cactagacaa gaccttttag actatggttt aatagctggg
     3901 gcacaagcaa aagaaaaacg agaaaaacta ggtgacgagt tgcgcatcgg ttacacgaac
     3961 ggcaaacaat tggaaaaacg tttgaagatg tttcgtatta cgccgaaaga actcgcccaa
     4021 gcaatgaaaa tagtggagga acaacatgag tgaatatcgt gaaattgcga caccctctag
     4081 aacaaaagag attttattga aacatggttt ttcatttaaa aaaagtttag gacaaaattt
     4141 tctgactgaa ccaaatattt taaggaaaat cgtagaaact gcgcaaatag atgaacatac
     4201 gaacgtgatc gaagttggac caggaatcgg tgcattgacg gaacaattag ccaagcatgc
     4261 aaaacaagtc gtagcatttg agatcgatga tcgtttgatc cctgtattgg ctgatacgat
     4321 gcaaccctat gacaatgtga ctattgtaca tcaagatatt ttaaagaccg atttgtcaac
     4381 agctgttaga gaaacatttc atgaggagct tccgttaaaa gtcgtagcga atttacctta
     4441 ctatattacg acaccgatca tgatgcattt tcttgaatca gatttggttg tggatgaact
     4501 agtcgtcatg atgcaaaaag aagtagctga tcgtatctct gctgaaccag gaacgaaagc
     4561 ttatggttca ttatcgattg ctgttcaata ttatatggaa gccagtttag cattcatcgt
     4621 accaaaaaca gtatttgtcc ctcagccaaa tgtagattca gcgattttga aactgacaag
     4681 aagagatata ccggcagtcg aagtgacaga cgaaaaagca tttttccgat tgacaaaagc
     4741 ggcatttcag caaagaagaa aaacgctttg gaataacttg cagcattcgt atggcaaaga
     4801 tgaccagaca aaagcatggt tagcaaaaag cttggaaaca gctggaatcg atccaaaacg
     4861 tcgtggggag actttgtcat tacaagaatt cgccgcatta agcaatgcga tgtcagaaaa
     4921 tatgcagtaa aaaaagaggt tgtgacagaa gttgtgaaaa agctgtcctg catcaagtaa
     4981 taagaacagc caaacaaaaa tagttcctca tgtttttcgt ttgtttgggc ttattaccgc
     5041 agatgcaagc ttttgaacac cgtttaagaa agaggttgtg ggcctgccgt ttaactctaa
     5101 gtcacaagga acaatttcaa aaaacagctt ctcatgtttt tgagttagtt ggtcttatta
     5161 cttgatgcaa gcttttgacc actattatgc cgaacaagga gatgtgacac aaattttgtc
     5221 acatctccta tttttgtttc ttgatcaaaa aattcttata cttgatactc acactcattt
     5281 tgtatggatt tagaaaagaa tatgtgtctt gacagttatt agtaagtgag ttatactaag
     5341 tcgacattta tgtgtcaaga catattagga gagagaaaaa atgagaaaaa attcagttcg
     5401 aaaactaacg atttctgctt tattgattgg gatgggggtc attatcccaa tggtcatgcc
     5461 taaaatcatg attccaccag cttcctttac tttggcgtct catgtgccat tatttattgc
     5521 gatgtttttt acaccaggag ttgcagtggc tgttgcgcta ggtacgacat ttggtttctt
     5581 tttaacaacg ccggttatca tcgcattgcg agcattgtca catcttgtgt ttgcgttgat
     5641 tggggcttgg tacttgcaaa agcatccgag tatcgtttta aaaaatggac agtttacttt
     5701 tgctaattgg cgtttccaag ggttcaattt tgtgatcggt gtgattcatt ctttagcaga
     5761 aatgctagta gtcagcatct tttactttat tggtaatatg cctgaaacat attattctca
     5821 aggatatttt tatacagtat tcatcttgat ggggattggt gggcttatcc acagtttagt
     5881 tgattataat attgcttatt ttatagcagg aacactcagc aaatattttg atatccccgt
     5941 atttacggca gcaaaaaaag aaaatcggaa agagtcgatt caagggtacg cgaagaaaac
     6001 gacatattag acatgatctc tcaaaaaaaa aactctcatc tagtaatgga taataaatcc
     6061 tctaagatga gagttttttg tcctaaaaag agctgcacca acatcttctg gcacagctct
     6121 aacagggggc aatttatgga agtttgatga cttgttctgt ttctttatca aagaagtggc
     6181 ctttgctgat gttgaaggca aggtcgacta tttcgcctgg tttatggaaa tctcttgcat
     6241 ctacttttga gatgaactca gtagaaccag tttttgtgta aagcatcgtt tcagctccaa
     6301 gtaattcgga aacgacaact tctgatttca ctacggcttc aggtgaagct tcaagggcta
     6361 cttgttcact gtggatgtct tctggacgaa taccgaagat aacttctttt ccttcataac
     6421 ctttttcaac taatactttg ttttttcctt caggaatacg aagcttaagc ccatgtccat
     6481 ctgtgataac accatttttc aatgtcacat taaagaagtt catggcagga gatccaataa
     6541 agccggcaac gaacatattg acgggtgtat cataaacttc tttcggtgta ccgatttgtt
     6601 ggataaatcc gtctttcatg ataacgattc ggtcagccat agtcattgct tctgtctgat
     6661 cgtgagtaac atagatggtc gttgtttcaa gacgctggtg aagtttggca atctcagcac
     6721 gcatggctac acgtaattta gcatctaaat ttgataatgg ctcatccatt aagaagactt
     6781 ttgcatcccg aacgattgcc cgtcccaatg ccacacgttg gcgttgtcca ccagataaag
     6841 cagctggttt tcgtttcaag tattctgtca gacctaagat ctctgctgca ttgtccacac
     6901 gttttttgat gtctgcttta tcatatttac gtaatttcaa gccaaaagcc atattatcaa
     6961 aaacagtcat atgaggatac aatgcgtagt tttggaaaac catggcaata tcacggtctt
     7021 taggtgccac gtcattcatg acttttccgc cgatatttag ttctccttca gagatatctt
     7081 ccagtcccgc gatcattcgc aaagtagttg actttccaca accggaagga ccaacaaaaa
     7141 cgataaattc acggtctttg attgatagat taaaatcagt tacagaataa ttttctgcat
     7201 tgtcatattt tttatatacg tttttcagag ccatttctac cataatacgt tccacacttt
     7261 cttttttagt tttattaact atttaaagta taaatgaaag cgttaactaa cttcaatgtc
     7321 atgttgaaca agaatttttt cttttttttg tcaatgtgct agttttaagc tttaataaat
     7381 atccataaga aataaaaact gatacactgg agtaaataag tatgttaaca atatcaaaag
     7441 gagtgtctct tatgaaaatc gctttgatcg ctcatgaccg aaaaaaacct atgatggtga
     7501 aattagcaac agcgtataaa acaatattgg aaaaacatga actttttgct accgggacga
     7561 ctgggcagag gatcacggaa gcaaccggct tgccgatcca tcggtttaaa tccggaccgt
     7621 taggcgggga tcagcaaatt ggagccatga tttcggagga tcagttagat ttggtgattt
     7681 ttcttcgaga cccgctggct gcacagcccc atgaaccaga tgtaacagca cttatccgtt
     7741 tatctgatgt ctatgagatt ccgttagcta cgaatatcgg aacagctgag gtgctactaa
     7801 gaggactgga agccggcttc ctagattttc gaagaatcgt ccatgaaata gacaagcatc
     7861 cactttcatt ttgatggata agcgtatgga aaaataacgt ttaatgttcg cctatatgta
     7921 gctataaaag ctaaacaaac tgataatatg cgatataata cgaacgtatt ggataatata
     7981 aacaataata ttagtttttc gtatttgcag taaatgtgaa aatatgctat agtttctgtg
     8041 ggaaaagatc ccaaaaataa tacagagtag aaaataaagc gttaagtgct aagaggatgg
     8101 gatgttgcct cttggacgaa ggacgtaatg ttcgcggttt tattttcgca ttcgctgcat
     8161 gatacgatgt agcagctctt aaaggagatg ctaaaaatga agaaacaccg gttagacgca
     8221 cgtatgattg ctattatggg cttattgatt gcattgatgg tcactttgtc ccgactggta
     8281 gcaatcgaga ccccatttat taaaatcagt gtcacattta ttccacaagt gatcatgggc
     8341 attctttttg gtcctttttg gtcagggata ggcgcagttt tagctgattt agtaggaatg
     8401 gccctcttct cgaaatctgc attctttatt ggatttacat tgaatgcatt tattgaagga
     8461 gcaatctacg gattcttttt ctatcgaaaa gagatcactt ggaaaaatgc cattctggct
     8521 acgcttagtg tcacgctgat catcaactta tttttgacac cattatggct tgcgttgatg
     8581 taccatgtgc cattgttcag ctgggtcgtt tgggcgcccc gcctgttaaa aacagtaata
     8641 tggttaccga ttcaatcaat tgccatttat tacgtaggac gtagtattcc ttacaaaaaa
     8701 attttaagaa gtttagcgat ccacgctaaa taaaaaacaa gcagtcttcc catcccagaa
     8761 gactgcttgt tttt
//
LOCUS       DAXWOO010000116         8715 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.117, whole genome shotgun sequence.
ACCESSION   DAXWOO010000116 DAXWOO010000000
VERSION     DAXWOO010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8715
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.117"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(244..1197)
                     /gene="menA"
                     /locus_tag="ACGE70_002079"
     CDS             complement(244..1197)
                     /gene="menA"
                     /locus_tag="ACGE70_002079"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFF8693702.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1210..2313)
                     /locus_tag="ACGE70_002080"
     CDS             complement(1210..2313)
                     /locus_tag="ACGE70_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFF8693703.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(2403..3338)
                     /gene="pplA"
                     /locus_tag="ACGE70_002081"
     CDS             complement(2403..3338)
                     /gene="pplA"
                     /locus_tag="ACGE70_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFF8693704.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            3640..5574
                     /locus_tag="ACGE70_002082"
     CDS             3640..5574
                     /locus_tag="ACGE70_002082"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFF8693705.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            6031..6447
                     /locus_tag="ACGE70_002083"
     CDS             6031..6447
                     /locus_tag="ACGE70_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFF8693706.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            6461..7027
                     /locus_tag="ACGE70_002084"
     CDS             6461..7027
                     /locus_tag="ACGE70_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFF8693707.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            7050..8036
                     /locus_tag="ACGE70_002085"
     CDS             7050..8036
                     /locus_tag="ACGE70_002085"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFF8693708.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(8191..>8715)
                     /gene="scm"
                     /locus_tag="ACGE70_002086"
     CDS             complement(8191..>8715)
                     /gene="scm"
                     /locus_tag="ACGE70_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFF8693709.1"
                     /translation="TSASSDSTDTTSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSD
                     STDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSD
                     TRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVI
                     GLRITFMSYKKSKR"
BASE COUNT         2565 a         1682 c         1764 g         2704 t
ORIGIN      
        1 tgttcaaaag ccagtttctc ggtattaagc caaatttatg caaaaatgtg gaaagccatt
       61 ttcacaaaat ttttcttaat actcgaaact aagcgacttt ttcacaacct cctgataaac
      121 ggtgttcaaa agcttgcatc tgcggtaata agcccaacca aacgaaaaat ataaggagct
      181 atttttattt ggctgttctt attacttgat gcagggcagt tttttcacaa cctcttcatg
      241 ctgttaaatg gcttgcataa tgattgttag taatagcccc aatacataac tggcattgaa
      301 taagaccata tttttgatgg aatagctgaa actgataggc tggggtaacg tttgcttgaa
      361 ttgatccaaa tgcttcttca caattggcaa ggtcaagaag gtgaccaaga tcggccattc
      421 atagataccg ctcaacaacc ccattaaaat cacgaaatag caagcataca tcaatagctg
      481 gaataagact gttcccgcag gacgtccgat ataaaaaact agtgtatagc gatgattttc
      541 aatatcacgg tcaagatccc gcaaattatt agccaacatg atattggcaa tcgtaaacac
      601 caagggtaag gaagctaata cgattgctgc tatagcccaa agatttccta ccaaatcaaa
      661 ttgtccagat gaaaagttga ctgtcagatc atagacatct gaaccgaatg tattgacata
      721 aacgaccatg gcaaaaattc caaggcccat agtcacccca ctgaaaattt cccctaaagg
      781 cattcgagat aaaggaatcg gtccgaatgt atagaatact ccaataaaac aacagataaa
      841 tcccatcaaa agtaaaagcc aacccgtatt gatcgttaga ataacgccga taacaaaaac
      901 aaatgcaatc atagctaaaa tcatgtttcg aatcaataca ggagatagat ttctgcggcc
      961 aatgacattt tcttcatact tatatacttc agaatgagcc ttttcataat ccatgtaatt
     1021 attgatagcg gtagtcgcta aatcaaaaac tagcattcca ataaaaaata aaaaagtgtt
     1081 tagccaatgt atctcatgaa aaaaagcgat agagaataat acccctaccg caaaaggaaa
     1141 tacactagcc acctttgttc gtatttcaac tacttctaaa aagattttta gtgtcatggt
     1201 tcgtactcct tattttagtt cagaacggtc acccatcgtg taatgggagt cctccgataa
     1261 tttgaaggtg tctttgattg gatcggtcac atatacttta tcgtcttttg tcacgaagat
     1321 cgcttccgtc ccgttattca attcgctttc gacgtattcc agtccttttt tcacacccat
     1381 tgaaaagact gctgtagaca acccgtcacc atcaattgat ttatctgtta tgatcgtgac
     1441 acttgcgata tcattgtcga acggataacc cgtttttgga tcaaacaaat gatggtattt
     1501 tttgccatct acttctaaat aacgctcgta aattccagag gtaactaatg ttttgttccg
     1561 ttctttaata gaacctaaaa cagatccgcg agccatgttc ggatcttgga tgccgactgt
     1621 ccagtcttgg ttttcgcctc ttgggctgtg gcctaagaca tagacattac cgcccaagtc
     1681 aacgatggca gtggtcactc cttgtttttt caagactttc actacttcgt ccgtaatgta
     1741 acctttggcg attgcaccta aatcgatgat cattcctttt tcttttagat aaacagtttt
     1801 ttctttgtca tttagttcga tcttatgata atccactaat ttcaatgcct gatcgatttc
     1861 ttcttgggaa ggttttctgg cgtcatcaaa accgattcgc cataattgtg tgattgcacc
     1921 aatcgccata tcgaatccgc cttgagaatc ttggctatat tcgtaggccc gcttgactaa
     1981 cgtgtagacg tcatcagaaa cttttactgg tttgatccca gcttgctcat tcacttcatc
     2041 gatctcagaa cctttttgat tgatcgtgat tttatcacca agttctttta ctcgatcgaa
     2101 ggcaggtttc aatgctgatt ctttgccttc atcatacaca cggatacgaa cgtaagtccc
     2161 taacaagaac tgttcatcag aatatggatc tttcaatatc ttggtagctg ctgaactttc
     2221 agtggttgtt ttatttgctg agttgctgca gccagttagc accataaaag agatgcccag
     2281 tgtgattaag gaaaatagta gttttttccg catctttttc ctctcttcat tttgaaaggt
     2341 gagacgactg cctaatctgg ctatcgtccc accctccaac ttttattagt atgattatgt
     2401 gattattttt ctgttacgat attatctact tcgattgttt gagtgtcgcc tttttcagct
     2461 gcattcacta attgttgtgc gtacatgatg aatgaatgtg agctgtgtgt agcacctgta
     2521 acaacttcta cattagcagg tgagccatct tcttcgccca ttgctttgac gaattcgtcg
     2581 ttcagagtag ggatgtaagt ttttggttct gtaccgcttt ttgctttcat attttcatta
     2641 tattctgtat catcttgttt tgatttaccg tctttgtcga tataatcaaa gtttgattca
     2701 gttactttgc cgccagcaac tgtcatttcg aattgcacac ggtagccgtt agagtagttc
     2761 ttttcttcaa gcttgtaagt gccgtctttc aaatcagcac cattgtcgat ttcgattgta
     2821 tctgtgttac cagcttgagc agcttggatc aattgttgtg cgtagttttg gaagctttca
     2881 gatgagtgag tagctccagt tacgacttcc acaccgcttg cgctttgtgc ttttaagaat
     2941 tggtcattca actcaggaat gtatttttct gggtttgtgc cagattttgc ttccatgttt
     3001 ttgttgtatt ctgcatcttt tgtttttgat tcgccgtttt cattgacgtt atcatatttt
     3061 gattcagtga ttttgccgtc ttttacaacc atttcaaaga ctgcacggta accatttgaa
     3121 taattttttt cttctaattt atatgtgccg tcttgcaaat ctccgccagc tacaactttt
     3181 gcagatgatt ctgttgtgct ttttgcagtc gaagattgtg ctgtagatga ttcagaagaa
     3241 gagtttgagc ttccgttgtc atctgcgccg caagcgccta acactagagc tgaaaatgct
     3301 aatacggcaa atccagttac aaaacgtttt ttcttcattt taatacccca ccattccatt
     3361 tctagtccac aatttttctt tgtgattcat tatacattct aatcttttta gcctataagt
     3421 caactatttt tgataaataa cccatgtaat atttcaaacg ttttctttca gcaaatttcg
     3481 tgaaaaaacg ctttatttta aaatttgaaa aaaatccaaa aggagttaga caaaagaaca
     3541 gaacatctat ataatatatg gagattgtgt aataagtatc aaactaaaag aattatcata
     3601 tattcattat atagatagtc tataaggagc ggatgatcaa tggcaaagca aaatattgtc
     3661 gttgtaggag caggatatgc cggtgtttca gctacaaaat acttggcaaa gaaactgaaa
     3721 aaagaagatg tagcaattac tctgatcgat cgtcattcgt accatactat gatgaccgag
     3781 ttgcatgaag tagctggcgg acgtgtagaa ccagaagcaa tccaatacga tctgcagcat
     3841 ttgtttgctc gtcaaaagaa tgtacaactt gtcacagaca ctgtaatcgg tatcgataaa
     3901 gaacgaaaaa tcgtcaagac caaattaggt tcctatcctt ttgatcagct gatccttgga
     3961 atgggcggag agccgaatga ctttggtaca cctggtgtca aagcacacgg attcaccctt
     4021 tggtcatttg aagattctgt aaggattcgg gaacatattg agaagacagt agcaaaagct
     4081 gcattggaac cagatgatga actgcgtaaa gccatgctga ctttcgttgt ctgtggttct
     4141 ggatttaccg gtattgaaat gattggggaa ctgatcgact ggaaaaatcg cctagccaaa
     4201 gattataaat tagatccaaa cgaaatcaca ctgatggtcg tagaggcaat gccgacgatc
     4261 ttgaatatgt tggatcgaaa tgatgctgca aaagctgaac gctatttgcg taagcacaac
     4321 gtggaattat tattgaatgc accaatcgta gaagtggctg ctgatcatat caaattgaaa
     4381 gacggacgca acattccgac tcacacactg atttggacag ctggtgtcaa aggaacttct
     4441 gacgctgctg actttggatt agaatcagca cgcggacagc ggttgatcgc caatgaatat
     4501 atgcaggcaa aaggctacga agataaaaac atctatatca ttggtgattt agtcttttat
     4561 gaagaattcc caggaacacc gacaccgcag atcgttcaag ctgctgaaca aacaggacac
     4621 acagccgcag cgaatatcgt ggcttcgatc aaaggcggag aaaagcataa atttaaagga
     4681 aactatcaag gatttatggt ttctattggt gccaaatggg gggtagctaa cctatttgat
     4741 aagatccatt taagcggatt tttagctatc gtaatgaaac atatcgtgaa tttgaaatac
     4801 ttctttgata ttcgttctgg ttattatatg ttccaatatt taatgcatga gtttttccat
     4861 atcaaagatg atcgcaacgt gactcgagga cattcttctc gttacggcaa tgtcttatgg
     4921 agtgtgccac ttcgtatttt ctatggttta gtttggttcg ttgaagcttc caaaaaaatc
     4981 attgggaatg gtgaagtgtt caaaccaagt acttggtttg gtgaaggatc atggttcact
     5041 ggaaacagtg tgttcccttg gacatgggaa tacaatgtaa cagatgttgc cagtggtgct
     5101 tctgctgctg gtgatgcaac cacagctgcc agcggagcag gggcaagtgc cggagaagca
     5161 gcgactcaag ctgcacattt tggtttgagt tatgcatacg gtgaagaacc aatggctgtg
     5221 attgagaagt caccagactg gttcacaaag atcatgcaag tgatcatgcc aaacaatgat
     5281 gtagcaatgt tcttccaaaa attcatggta ttctttgaaa ttgccttggc attagcattg
     5341 attgctggat tattcacatg gctatgcagt gcgacaacga ttgtattggt gatcacgttc
     5401 tgcttatcag gcatgtttta ctgggtaaac atttggttca tttttgttgc tttcgcattg
     5461 atgaacggtt ccggacgtgc gattgggttg gatcgctggg taattccatg gctacaacgt
     5521 acactaggta attggtggta cggtagacca agttcccagt acggcaagaa ataaatagat
     5581 aaagaacgtt atttgaatgc aagatttcaa tagaaagggg ctgagacaaa ccgtcacagc
     5641 ctccttttta tgtaaaatta aaggaataag atgcccgaga taagcctcca tgagcaagac
     5701 acaggtgtca caacctctgt tacaacctct tgttataaac ggtgttccag aagtagcttc
     5761 ttcgaaaaat aagccgaaat ttcacaaaaa cttgaaaagt aattttcgta aattccttct
     5821 tatttcttga agttgcccac ttctgtcaca acctcttgtt ataaacggtg ttccacctgc
     5881 tgcttctcgg taataagccc aaagaattag aaaacatgga aagctgtttt ctattcttcg
     5941 ttcttattac tcaaagcaag acacaggtgt cacaacctct tgtttttatg ctaaaattgg
     6001 gtgagttaat ctggaaaaag gagaacaaaa atgagcctta aagaatttgc tgaaaaaagt
     6061 ctgctcaaac cttgggatgt ttttattatt ttgcttttga ctgttctttc ttttttacca
     6121 gtcatgattt ttggtcttca gcaaaacacc gcaacagcag aaaaagaagc ggtactgcgt
     6181 gtggatggca cagaaatcaa aacattccct ttgatggctg gagaagagag caacacatat
     6241 acttacaaag atgaacacgg agattataac ttgatcgaga tagatggcga tcgagtacgg
     6301 ataaaagaag cagattgcga tgaccagatc tgcgtacgtc gaggctgggc aacgaaaaat
     6361 ggcgaaacca tcgtctgttt gccacataaa ttagtgatcg aagtccgttc aacagatgga
     6421 ggtgacgaag acagtctgat ttattagacc ttcacacacc atgagtaaat tacaaaaaat
     6481 gatttatatt tcgctacttg ttgcacaagg agtgatcatt ggtttattgg aaaatatgat
     6541 cccttatcct ttttcttttg cgcctggtgc aaaactaggc ttagccaatc taatcacgat
     6601 cgttgccatt tttaccatga aaaaacaaga cagcttctta ctactttggc tacgactatt
     6661 cctgactact ttgctaggcg gaacgatttc cacattcctt tatagtatga gtggtgcttt
     6721 gcttagttat tttggaatgt tattggtcaa gcaattagga ccaaaacgtg tcagtatcat
     6781 cggaattagt gcggccggcg gtttcatgca taatgtcgga cagcttttga tggcttcttt
     6841 cattgcacaa tcatggacag tcatgttgta cttgccagtc ctttcagttt tagggatttt
     6901 atcagggatt gcaatcggga tcgcagcgaa ttatctttta cgacatgtgc agacacttca
     6961 acgattccaa aaagattatg agcagggaac caaacaaaaa tggagcccaa tgtttaccaa
     7021 aaaataatcg tcacacaagg agttttttta tgaagctaca tccaatgtgg gaaaactatc
     7081 ctgctttaag taaagaattg acagaaacac taaaacggat ggaagaaact gtccgcctga
     7141 aaaacaaacc tgtcgaacaa gccatcaaag aaacgatcca tgccggcgga aagctgttgc
     7201 gtcctgccta tcagctattg ttctcacaat ttggaccaga gcaagatcga gacaaagcca
     7261 tcgctttggc agcttctatc gaaatgctcc atacggccac actgatccat gatgatatcg
     7321 ttgatgaagc ggatctgcga cgtggtcagc ctaatatccg tagtcagttt ggtaatgtcg
     7381 tttctgtcta tgcaggagac tatttatttg tctgctgttt taaactattg tctgattact
     7441 ccacttcctt gaaaagtctc caattgaatt ctcgcagtat ggaaaaagta ttgaatggcg
     7501 aattagggca gatggatgat cgctataatt atgaactttc tgtaaaagaa tatttggaaa
     7561 atatttcagg taagacagct gaactgttcc aattaagttg ttctgttggc gcttttgaaa
     7621 gtggaacaag cgagcgcttc gcaaaaaaag caggagatat cggactttct atcgggatgg
     7681 cctttcagat tatcgatgat attttagatt atacaaaaga gagccaggaa atcggcaaac
     7741 ctgtactaga agacatgcgt caaggggtct attctttacc gttgatctac tctttgcaaa
     7801 aaaacaaacc aaagctacta ccttatttag agaagaaagc ggcaatgaca gaagaagatg
     7861 tcgacgccgt acgcaaaatc gttgaacata caggaggcgt ggaagaagct aggaagttag
     7921 cggcctctta cacagaaaag gctttgaagg aaataaagaa attacctgcc acctctcttc
     7981 gaacaaaaga gaatctcttt tcccttaccc aactgatttt ggatcgcaaa gactgaatag
     8041 ggaaaggttc gatacgtaaa taaatttctc agaaggaaat ctacgtgttt tttgacaaat
     8101 tgaattgtag ttcttgtatt tatgataaaa agcaaaaata aagagcaacg tccgttagcg
     8161 acgttgctct tttttatctt gaatattttt ttaccttttc gatttcttat aactcataaa
     8221 agtgattctt aaaccgatta caagtaatat aacgcctgcg agtgttatcc aattattcga
     8281 ttgacttcca gttttaggaa gtgctctttt tgatttgttt ccattgtcat tgcttgtttt
     8341 attagttaag ttaaagttcg tcctgttatc cgaacttgtt tttgagaagg agacacttgc
     8401 atgacgtgta tcgcttgtgg tagacgacgt gtctgtacta tcgcttgaag ttgatgaatc
     8461 tgagctatca cttgtggtag aggatgtatc tgtactgtca cttgaagctg acgtgtccgt
     8521 gctatcactt gtagtggtag atgaatctga gctatcactt gtggtagagg tagtatctgt
     8581 actgtcactt gaagctgacg tgtccgtgct atcacttgtt gtggtagatg tatccgtact
     8641 gtcgcttgaa gctgacgtgt ccgtgctatc acttgtggta gaggtagtat ctgtactgtc
     8701 acttgaagct gatgt
//
LOCUS       DAXWOO010000117         8542 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.119, whole genome shotgun sequence.
ACCESSION   DAXWOO010000117 DAXWOO010000000
VERSION     DAXWOO010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8542
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.119"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(122..412)
                     /locus_tag="ACGE70_002087"
     CDS             complement(122..412)
                     /locus_tag="ACGE70_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFF8693710.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(548..727)
                     /locus_tag="ACGE70_002088"
     CDS             complement(548..727)
                     /locus_tag="ACGE70_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693711.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(724..1074)
                     /locus_tag="ACGE70_002089"
     CDS             complement(724..1074)
                     /locus_tag="ACGE70_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693712.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(1067..2392)
                     /locus_tag="ACGE70_002090"
     CDS             complement(1067..2392)
                     /locus_tag="ACGE70_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFF8693713.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            2842..3456
                     /locus_tag="ACGE70_002091"
     CDS             2842..3456
                     /locus_tag="ACGE70_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFF8693714.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(3621..3863)
                     /locus_tag="ACGE70_002092"
                     /pseudo
     CDS             complement(3621..3863)
                     /locus_tag="ACGE70_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4067..5386)
                     /locus_tag="ACGE70_002093"
     CDS             complement(4067..5386)
                     /locus_tag="ACGE70_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HFF8693715.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            complement(5520..6128)
                     /locus_tag="ACGE70_002094"
     CDS             complement(5520..6128)
                     /locus_tag="ACGE70_002094"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693716.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSLNNSYF"
     gene            complement(6766..7023)
                     /locus_tag="ACGE70_002095"
     CDS             complement(6766..7023)
                     /locus_tag="ACGE70_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFF8693717.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(7016..7285)
                     /locus_tag="ACGE70_002096"
     CDS             complement(7016..7285)
                     /locus_tag="ACGE70_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFF8693718.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            complement(7416..7595)
                     /locus_tag="ACGE70_002097"
     CDS             complement(7416..7595)
                     /locus_tag="ACGE70_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693719.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            7693..7929
                     /locus_tag="ACGE70_002098"
     CDS             7693..7929
                     /locus_tag="ACGE70_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693720.1"
                     /translation="MKQNTSIAKNTTKFKCTAAAWRISISPTNRTNNRGMILFIWISF
                     FLLGYRVLPAYFAPPKRRGRLERLVRNKNHSLSY"
     gene            complement(7976..>8542)
                     /locus_tag="ACGE70_002099"
     CDS             complement(7976..>8542)
                     /locus_tag="ACGE70_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693721.1"
                     /translation="EEQLYLESETSQTKVTVSHNRLDVYKRRPETVKKGTELLRLETE
                     KYIMNVYLERSELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATID
                     DYYLVFTRFLTCGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTY
                     TYDDTEEIQNELDRKRMPYQRFERKKRQ"
BASE COUNT         2605 a         1611 c         1330 g         2996 t
ORIGIN      
        1 ttactaaact gtctatattg ttactaataa ttcccttctt atagtgcatt ttcttagaaa
       61 aaaacaatat tacgatatca tgatattaag atatcgtaat attatacaat tgtgattgat
      121 attatatatg tttttttatt tttttatcat gtttaatctt gaaggaatca tattcttttt
      181 ctaaagacag gagcttttca taaacatcag acatgtttaa actagaggcc aggcttaaat
      241 cataaacgaa tgaagcaggt aatgatttta ttctacgttg tcgggttatc cagcttgcaa
      301 ccgttccttg tgggtaggca tataactcac aaaattgttc tacagtgatt cctaaattct
      361 tgataatgta aacattgatc gggtgcgagt aaacaaatgt attgatagac atggtgcgta
      421 agcctacttt cttttattat tgcgtttgca ataatagttt aacacacgca ttattctagt
      481 atctactcct atttgtaaaa acaaaaaagg attcatagag ataactctat agaatccgta
      541 aaatacctta tatatataac gggaagatat aaaacctaaa attatgataa cctgctgcag
      601 cttctacata gaaagagaag cgctcaacac caacctcatt tatcaaacgt tggccataac
      661 cccaaatctc ttctgatagc tgatcaaagg ttatatactt tagctcatat tgctggataa
      721 tgttcattct aatcgactcc atttattatc ctggtaaaat tcgatatgac gtatttcgtc
      781 gtagtgaatt ttgtcttttc caacgtagat ccccagctca tcactaccta agatcttgcc
      841 aatgatatct tctggatagt atccttcagc gtttcgttct tctttttgaa tagatactga
      901 tctatttttt atgactgcag cgttcaatac ttccgcaatc tcttcagtcg tcatttgttc
      961 ttttgctaaa ttttgactgg ttcgttgttc attgtcggca tcaatttctt cagtatgatc
     1021 agataaataa aaccctgccc actttaaacg tccacgatca ttgtaatcaa tcatttttaa
     1081 ttcccccatt tccgcccgca tgaccaccaa caagtgtgct tctagtaatg cttctagcgc
     1141 cttctaacat tgaagaagca tgtacaatcg acgtaaaacc atatttttgc cgaatcttgt
     1201 caataatttt atctaaattt tcttcgtcaa tttgcttttg tggatcacta aataaatcca
     1261 gctgaagcga atcggtgtac acaagcttgc tataggttat tccaacatgc cttacttctt
     1321 gtccttcata atacttatca aacaagaaaa gacaataatt ctgcaattcc tttgtattat
     1381 ctgtgatagg gatcttcatc tggtgtgaaa accctggtct cgtttctaag atcgacgtgc
     1441 caatgtgtaa atggacacaa cctgttttac agttatgctg cctaatgcgt atcgctactt
     1501 gttccgccat ttcttttacc accagctcga tttcatttct tctagtataa tctcgtggta
     1561 acacttgtga atttccatat gatttttcct ttgtcggttc tggtgggatt gctatatctg
     1621 ttcgatcgat cccattagca tggaagtaca gctgtaagcc aatcacacct agtctattct
     1681 taatcgtata aggattccag ttagcaagat ctctaatgct cataatcccc atctgattga
     1741 gtcttttttt cattcgagag ccaattcccc aaaagtccgt catttcagga atattccata
     1801 ctttttcagg aacgttttcg tacgtccatt ccgcaacaaa tccttcatta tgctttgctt
     1861 cgttgtctaa ggctagttta gccaaaaggg gattatctcc gacacctact gtagcaatca
     1921 atcctagctc ttctttaata ttctcctgga tcatttgagc cagcttcttt ctccgttgac
     1981 ttcgtgttcc ttcagtcgta aaaagattca gtgatcgggt cacttttaag attgattcat
     2041 cgatcgagta aatcagtaga tcttcatcag ccacatatct tctgaaaata ttatttacct
     2101 gcatatttcg cttgatatat aggttcatac gtggtggaac aacgtgtagt gttttaggaa
     2161 atggttgtgg taagtcacgt ggtctactca catttgtaat accataccgc ttttttgctt
     2221 caggagaaga agctaatatc aatcctgaac cagtattatc agctcgactc ataacaacaa
     2281 gttctgttgt taatggatct aaatttcttt ctatacattc gacacttgcg taaaagcttt
     2341 tcatatcgat taaaaagtaa tcatttactg gctcttttga ataatccatc ataaataaca
     2401 ctccattctt tcacattata caaacatatg ttcgatattg taaagaagaa atagctaaat
     2461 acataaaaaa agaagcatag gtatgctatc accctgcttc ttcttttgat ttgtatgatc
     2521 aatttattct atcactttac taattaattt gaaaatctaa taaaaactct cactctaaaa
     2581 gagtgagaga ctgcaaatta gagtacggac ttttttggag ttccgttctt taatattaac
     2641 aaatgaataa aatccttaca acatttgaga ataagttatc agtagacact tacaaaaaat
     2701 tctcatacta tttcctccat cattagtttt tgtctatata aggtttaccc taaatagaca
     2761 ctggtataac attgaaaaat atggcttcct cgtgtatgat tagggtgtat tttaaatagt
     2821 tgcagaaagt aggtaaaagt tttgagagtt gcatacatac gtgtctcatc gatcgatcaa
     2881 aatgaacaac gccagattga agaaatgaaa aaattcggtg cagagagaat tttcatcgag
     2941 aaacaatctg gcgcaacgat tactcatcga cctgttttcc aagaagcatt agattacgtc
     3001 cgagatcaag atatttttat tgtggaagct attgatcgac ttggaagaaa ttatgacgag
     3061 atcatcgctt cagtcaatta cttgaagaag aaaaatgtga agctgatcat tactagttta
     3121 ccgatcatgg ctgaagcaat tggcaatcct ctcctggata aattcattaa ggatctgatc
     3181 atccaaattt tggccatgat cgctgaacaa gaacggactg aatcgaaacg tcgccaggca
     3241 caaggaatta agatcgcaaa agcaaacggc gtctacaaag ggcggcccaa attatacagt
     3301 gcagacgcaa aagatcccca acgtcgcctt gtttacaaaa gcatcgttga agatcttaaa
     3361 aatggtgttg cgattgctaa gattgctaaa gactacaatg ttacgagaca aaccgtgtat
     3421 agaataaaaa aagacagcat ggtcaatgac aaatagttga tcatgctgct tttattatct
     3481 tctcatcttt aatttcctac atataattac tatctataaa attaagtctt actcagaaca
     3541 gataaagcat aaaattccaa caaaccattt tttacttaac taaataaaga gtctgttaat
     3601 ataatcttca cttatttacg ttaatcccct actgtatttg ttggttcctt atctatattt
     3661 tctacaaaat ccttattatc ttgtgattta gcttcgtata attctttgta tgtcgacaca
     3721 ttggttatac taattcctat agctagttct tgagactcta cttttctctc tagctccttt
     3781 gacaaagtga tttgagattt atttggttcc gttggagtca attctataga ttcttcataa
     3841 aaatctctat acataaagct atccctgaat aattcatagc tttaattctt tggcacattt
     3901 tattgaaata tgtatcacta atatccctat caactgattt tgactaaaaa gttacttcaa
     3961 acattcactg ttcaaaaata ttgaggaata aattttgggt gtaaggcatt ttgagcatac
     4021 ttctccctgg ataacttccc agtaaaaaaa tcgttagatt gttggattag atccattgac
     4081 tggctcgcct caggcgagca aagaccctgt agaattcatt ctgatacaca tgataactag
     4141 ataatttgag atagactcgt ctacccgttt ggactaattt cccagcaact ttcagaaact
     4201 tcaatcgaat agaatgaatg gtcattccct tttggacttc ttcaaagcca atcgttctta
     4261 agaagttgac taagttgtaa gctatcaagc tgagcatcat tcggacatga ttctccaaaa
     4321 agcgaggact gtctgttttg tcaaagtaaa agccagcttt cgcttcttta atgaagttct
     4381 ccattgtgcc acgtttggca tagatagaaa agatggtatc aggagaaaca ttttctgata
     4441 gattcgtcac gataaattca tgtcgaaaga gtagttcgcc cgcttcacgt gttgagcgta
     4501 tacacacgcg acgactttgt gaccaggatt gtgcttgata agtggtcgag aagtactgaa
     4561 cttctcgttc ttcccacttt tgattatcgc cataaagtac tgatttctca gctatttgac
     4621 ctagtctacg attattcttc agtcgaataa cataatgact cttttttgat tcacacgaat
     4681 catacacacc aggtgtagcg aacccgctgt ctccacgaac caagatgtca gtgtttggta
     4741 gagagtgatt atagtgctct aataaaggtg tgagaaactc ctttacgcct tttgatgtat
     4801 attgatttcc tgaacgtagt tcagctttta agaagtcacc agtcaatccg tcaaaagcta
     4861 ccaatggatg gtaaccatag gtttggtagt gtgtattata attcgtttgt tcttggtgac
     4921 caaatgtatc tgaatgggtc gaatctaaat caatgattaa ttccgtatca ttacggatga
     4981 ggcgtgcttt atcgataagt tcttggttca aagcttgaag ttcatggata ttctcctcag
     5041 atagtcgatc taaaaatcgg gaaagtgaag attgagaagc gagttctttc ctatctaaaa
     5101 cagctttaaa cacaggatct tgtctcagga gattagctgc agaatcagct gagtaaccag
     5161 caattaattg cataatgaac tgctcaagta tggataagtt gtcatgagta aaatacgctc
     5221 gttcgtcttc aatgtgaatg tgttgcttag ccagttcaga aaaaccaaag atgttcatca
     5281 gctctttcac taggacgaga cccgaatcac tcgataattg accacctgta tgagaaatag
     5341 tgatatttga attgaatttt acttggtttt tgtgtaagct agtcatgaaa agaactcctt
     5401 tcttttggtt ggtttagtca ctttaaccat agcagaaagg ggttcttttt tcatcactta
     5461 acgggtgaag atgaaaaagt aattgtaagc tgccgtgagg cagtttcaca tggttttaat
     5521 taaaagtatg aattattcag gctatcatta attttataaa aaaaattaaa tgtttttagc
     5581 actgccatat aacattcaaa acattcttct atagttggtt tacttccttg aggagaatga
     5641 accaatttat tacgtttttc tatgaaattt ttaatttcct ttcttatttc tttcggaaac
     5701 tcaatattaa agtacccttt aaacatgctc cctaataatt tgtctacagg gggagattgt
     5761 aaattctcaa atattagagt taaatcaggt ttctcttgtt gataaaattc ctttacccct
     5821 atttcacagg ctgtaattat cgaaataaac ctatctctag tatttgatat atttttactt
     5881 cttttaagga gtataagaga aaaggggata ctttcgtgtc catgcatgta attaactaat
     5941 tgttgagctt cagaagttat atctctaatt atatagttaa cggaattagg aagtgtcttt
     6001 aactctaatg aaatttctat ccaattacta tcttctaatc tgtatatgct cgaagtataa
     6061 tttgattcgt cagcaagcat cgctacatcc caaacactga tcataaaatc ttttaaacgt
     6121 tcaatcattt cagcaatatt gctaaaatca gctaattttt ctttagaata aaaaaaaaca
     6181 tttatatcag aatcaataaa ttctattttt ttattactat atcgtcatca ttatcttgac
     6241 catttatgtt tattgttccc tgagtgggga gtacttcttt caaattcaag ttaaatttat
     6301 attccattac ttctcctcca atatataaaa attacttaac tgtatccaaa tttaatatgt
     6361 aaataaatca ttaattttta gctaaattct atgagaagtt tatttttgtt gtttttgcta
     6421 attaactaaa actagtacaa aaaatttaaa atagattatt aaacctattg cttattctat
     6481 tcagtcatat atataagcgt catcctttag taactaacat cccacactac aatagtatta
     6541 taacatatct attcaacttc aaattgttga aatttaagcc atttatattt acttttcctc
     6601 aactttagaa atgattttac taaccttata tccgtaatac gcgtaacaaa cacattcatt
     6661 aataacaatc aacactaagc tatattagct ttttaaatga ctagggtaaa taaaataagg
     6721 atcaaaaact tttaagtttc tgaccctttc cttacttccg attggttaat agtgatcttt
     6781 tgcagaataa ataaatatcg tttcattttc aactctatat atcagtctat gttcatctgt
     6841 aattcttctg gaccattttc cagataaatc atgctttaat ggctcaggtt ttcctaatcc
     6901 agcaaagggg gaacgatcga tatcttttat taacttgtta atctttttta tattgctttt
     6961 gtttccttgc tcatgccaat aaagataatc atcccaagca tcatcagacc aagccttaat
     7021 catcagattc aacctcgatt aagtcatgtg ttttaaatgc acctttggag aattgttcat
     7081 ctcctcgacg aattttttcc atgacgtaat tattagaaag tgttctcaac gtttcttgca
     7141 tagaatcata atctcttttt gataatacaa caactgtatc ttctacatct ttacttgtta
     7201 caataagtgt ttcagcatcc tcattaactt gtttcatata actacgtaaa ttttggcgga
     7261 aatttgaata agctactgct tccattcctt tcacccctta tttatttcta taaaacaatt
     7321 gtacattata ttgtacaatt aagcaaatga ttaatattct aacttcttat aattaaaagt
     7381 gctatcactt ttagttttta ggataaaaag tgacgtcact ttttgagtgc tggtacctca
     7441 aatcctgctt tttcttgggc aatcaataaa tctaacgcaa tacacataat cctggattca
     7501 cttttgccgt acttctgctt tagttgctgc aattcttttt gtctcttttc atccaacgta
     7561 acgggtaacc gtttatttcc tattgcaatt ggcattagta ctctctcctt tcatcgaaat
     7621 aaccctatga aaaatatggc tgcaatcatc ttgaatagta gactcacaag ggctaataca
     7681 aagcgccaaa agatcaaaca aaataccagt attgccaaaa atactacgaa gtttaaatgc
     7741 acagctgcag catggcgcat aagtatttcg ccaacaaata ggactaacaa tagaggaatg
     7801 attttattca tatggatctc cttctttctc ttaggttatc gggtgttgcc cgcttatttc
     7861 gcaccaccta agcggcgggg gcggttggag cgattggtgc gaaataagaa ccattccctt
     7921 agttactagg ttttttgtcc ttatttcaca cattctcact tttcggtata aatgattatt
     7981 gccttttttt cctttcaaat cgttgatatg gcattcgttt acggtcaagt tcattttgga
     8041 tttcttctgt atcgtcgtaa gtataggtgt ctaaaaaatc agttaaagtc gtgtaccagt
     8101 cgtcgcctgg gttatctact tcttctacat attccctcaa aaatgcttcg ggtacagcga
     8161 gttcataatc cattcccatt aacacgatag attccgttcc acacgtcaaa aatctagtaa
     8221 atacaaggta ataatcgtct atcgtcgcat ctttgctgtt tagctcttcc atttcttctt
     8281 ccattaattc catgctctca aaaattgaat aatattctga ttctgagtaa tagtgtttcc
     8341 tgcaaatcgt gtagctgaca aagttttcga ccagctcact cctttctagg taaacattca
     8401 tgatgtattt ttctgtttct agtcgtagaa gctccgttcc tttttttacc gtttcaggac
     8461 gacgtttata cacgtctaaa cgattatggc tgactgtgac cttcgtttga ctcgtttcac
     8521 tttccaaata caattgttct tc
//
LOCUS       DAXWOO010000118         8525 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.120, whole genome shotgun sequence.
ACCESSION   DAXWOO010000118 DAXWOO010000000
VERSION     DAXWOO010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.120"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            476..1339
                     /locus_tag="ACGE70_002100"
     CDS             476..1339
                     /locus_tag="ACGE70_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFF8693722.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="ACGE70_002101"
     CDS             complement(1468..3090)
                     /locus_tag="ACGE70_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFF8693723.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="ACGE70_002102"
     CDS             3339..3860
                     /locus_tag="ACGE70_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFF8693724.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACGE70_002103"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACGE70_002103"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFF8693725.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="ACGE70_002104"
     CDS             5941..6759
                     /locus_tag="ACGE70_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFF8693726.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="ACGE70_002105"
     CDS             complement(6862..7059)
                     /locus_tag="ACGE70_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693727.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="ACGE70_002106"
     CDS             7228..7806
                     /locus_tag="ACGE70_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFF8693728.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="ACGE70_002107"
     CDS             7901..8038
                     /locus_tag="ACGE70_002107"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693729.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         2586 a         1523 c         1672 g         2744 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta gtttttccac agtggataac ttcgtaagag ataaaaataa aaaattagtc
     8221 aaagaagggc gtatagattt cttttgatag gagatataca acagaaatga ataaatatta
     8281 acgtgttttc aaacagtata aaacggtgtg gtttttggaa taaaaaaata acatatctca
     8341 ttttacgctt ttgttatttt atacttaaaa aataacgaca cagaaaatac cgtgtctcag
     8401 atttctctac tacttctaaa aactactgtt tttttccttt gaaatgcctt caaaaagtag
     8461 gatcaggcag aatatactgg cagaatcaag gagaagggag cctaaataag gagaaaaacc
     8521 ataga
//
LOCUS       DAXWOO010000119         8505 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.121, whole genome shotgun sequence.
ACCESSION   DAXWOO010000119 DAXWOO010000000
VERSION     DAXWOO010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8505
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.121"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(116..874)
                     /locus_tag="ACGE70_002108"
     CDS             complement(116..874)
                     /locus_tag="ACGE70_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFF8693730.1"
                     /translation="MDTKLSESEKYLWDFIQSHILEIPSYSIVKLSEYANVSTATVVR
                     TMKKKGYEGFTSFKHSLKEKENTNINFAALDKVDEGIKTAILKNEQEVIRTINMVESG
                     NIEDAIQRIKAARRIMIFARGFSELIAQEMMVKFQLTGKYCEMHTDPNIIKNISRKLS
                     KDDIVIFVSLNGETKELTTAAENCYKAEIGTILLTANRHSSLMEHIEIPFVGFKSEGS
                     FFPDYEVRSRLPLSILARILLDSYALRTSGFTQH"
     gene            1042..1977
                     /gene="pfkB"
                     /locus_tag="ACGE70_002109"
     CDS             1042..1977
                     /gene="pfkB"
                     /locus_tag="ACGE70_002109"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFF8693731.1"
                     /translation="MSVYTCTLNLAIDLFIETDEMKPSQVNRTNDDDIQANGKGVNVS
                     LIMKQLGIDSTALGFKAGFTGNYIEDYLHQKDISTDFISVPGMTRINVFTQVNKTNEE
                     FKLVNKGPEIPQEQIKTFLNQIQKLQADDYLCVSGSLPRGVSPEILIDISRICFENKV
                     HLIIDSSYPEIIDCLPYQPFLLKPNEEELQSWYNVELSDREGYIHYAERLIADGAKNV
                     LLSLGGEGGIFLNKDIVYSGNSPKGKVVNTACAGDTLLGTFLAGYLKGEQVDINLKKS
                     LAAGSSTAFRKGLTDFSDVAELEKQIEIKEGASKK"
     gene            1974..3419
                     /locus_tag="ACGE70_002110"
     CDS             1974..3419
                     /locus_tag="ACGE70_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFF8693732.1"
                     /translation="MTTYKIVAATGCPTGIAHTYMAQEALEQAAKRKGVSIKVETHGQ
                     VGIENELTPEEIKNADAVIIAADKDVQAERFAGKRVIDVPVSKGIKDADQLIEDALAG
                     KGSITAENKTVDAAGEKAEMGVAAGIGHSIYKNLMNGVSHMLPFVVAGGVIIAISFAI
                     WGVYSFDPNDPTYNPTAAMLKGVGDAAMGMMVPILAAYIAEGIAKRPGLVVGFVGGLL
                     ANSGGAGFLGGIFAGFLAGYFILLLQKVMKGLPKQLDGLKAIFLYPVIGVLFVGVVMS
                     LLANPMEAVNQGMMNFLAGFQNSNPLILGIIVGCMSAFDMGGPVNKAAYVTGTALLAQ
                     GNHTFMAGVSAACIAPPLITGFAALFFGKYFDTNDRNAGLVNFILGSTHITEGAIPFA
                     AKDPLRVLPTMMLGSSIAAVLTYMFGVQVPAPHGGFLVLPVVTGKVAWVGSILLGSIV
                     GGVILGLIQKRSVEKREAEAAAVEMKEATNN"
     gene            3447..3902
                     /locus_tag="ACGE70_002111"
     CDS             3447..3902
                     /locus_tag="ACGE70_002111"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFF8693733.1"
                     /translation="MTLIKTNKVFMNKNFTTQDEVMNFLADQSIVLSISEDRDAVYEK
                     LMEREEESTTGMMDGFAIPHAKDASIKDADIVIVRLNGKVDWKSLDGEGTDFVIALFI
                     PDSEAGTTHLKFLSQVARLLMHKDVTEGLKQAQSAEEIAELLNQKLEEN"
     gene            3921..4895
                     /gene="lacD"
                     /locus_tag="ACGE70_002112"
     CDS             3921..4895
                     /gene="lacD"
                     /locus_tag="ACGE70_002112"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFF8693734.1"
                     /translation="MPLTEKKKTYIRQLSNESGIISALAIDQRGALKKMIGKFQNKSA
                     TDEQIINFKKIISSELTPYASAILLDPEYGLPAAEVRDKNAGLLLAYEKTGYDASTPG
                     RLPDILSIWSVKRLKEQGADACKFLLYYDVDESDDINDQKKAFMERIGSECVAEDLPF
                     FLELVSYDANDPDTSSKEYALKKPHKVNEMMKEFSKPRYGVDVLKVEVPVNMNYVDGY
                     TKNEVAYTKEEAAAYFKEQSETTNLPFIFLSAGVTAELFQETLKFAKEAGSTFNGVLC
                     GRATWANGVEPFVTKGEYAAKEWMQTQGRKNIEELNNVLKETATAIKL"
     gene            complement(5006..5567)
                     /locus_tag="ACGE70_002113"
                     /pseudo
     CDS             complement(5006..5567)
                     /locus_tag="ACGE70_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            5711..6325
                     /locus_tag="ACGE70_002114"
     CDS             5711..6325
                     /locus_tag="ACGE70_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8693735.1"
                     /translation="MELAFKQLEKKDYKKAIQFAIKGMHFDWYTKNKLLLNLYGRYFW
                     YLELNRATQIIALYADDELAGVLLADIKGKSKKHHSFSQKLYVKLFDFLQNAFVKDSK
                     GGYDDTNDELLSDYLKKHSPDGEIVFLAANPDLKIKGIGSKLLKEFERREQGKEVFLF
                     TDSGCTYQFYERRGFERKKEKDNVMKILNKEVDLRCMLYSKIIQ"
     gene            6566..6949
                     /locus_tag="ACGE70_002115"
     CDS             6566..6949
                     /locus_tag="ACGE70_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFF8693736.1"
                     /translation="MPKYKEIALALKERIVEGKYQEGSKLPEQESLAREFKTSRVTIR
                     KAIQLLIDEGLLYTRRGSGTFIRSNIRQDNENVTQVNNVFGTSSQENTNVTSKIIRFD
                     VRFPTDYERRLSATYFRSPSCKSWV"
     gene            7312..7821
                     /locus_tag="ACGE70_002116"
     CDS             7312..7821
                     /locus_tag="ACGE70_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFF8693737.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILEVFAEAELYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            7921..>8505
                     /locus_tag="ACGE70_002117"
     CDS             7921..>8505
                     /locus_tag="ACGE70_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693738.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEVRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLER"
BASE COUNT         2806 a         1328 c         1788 g         2583 t
ORIGIN      
        1 atagaaggag caatgataga aagcacgaga atacaaatag cgattaaata tttttctaga
       61 caaccttagt taattcacac agatatattt taggtacagt cttacagata tagagttagt
      121 gctgtgtgaa tcctgatgtt cgcagtgcat aggagtcaag taagattcta gccagtatag
      181 aaagtggaag tctggagcgt acctcataat ctgggaagaa ggagccttct gatttaaagc
      241 caacaaaagg aatctcgata tgttccatca aacttgaatg acgattagca gtaagcaaaa
      301 ttgtcccgat ctcagcttta taacagtttt cagctgcggt agtcaattcc tttgtttcac
      361 cgtttagtga aacaaagatc acgatgtcat ctttggataa ttttctacta atgtttttta
      421 tgatattggg atcagtatgc atctcacaat atttaccggt aagttgaaat ttgaccatca
      481 tttcctgagc aatcaattct gaaaaacctc gggcaaaaat catgattctt ctagcagcct
      541 tgattcgttg aatagcatct tcaatattcc cgctttctac catattgatg gtgcggatta
      601 cctcttgctc atttttaaga atagcagtct ttattccttc atcaacttta tcaagagcgg
      661 caaaattgat atttgtattt tctttttctt ttaacgaatg tttaaaagac gtaaacccct
      721 catatccttt tttcttcata gtacgaacga cggttgctgt tgaaacgttg gcatattcac
      781 ttagttttac aatggaatag gaggggattt cgagtatgtg actttgaata aaatcccaga
      841 gatatttctc cgattcactt aattttgtat ccatattttg cctccgatta gttaggataa
      901 ttctattgta aagcgggagt ttcctttact tcaagtttat ttaatatatt ttgtaaaagt
      961 tttcaatgaa tttagtcatt tatgaaaaca tttacattcg atcttgatac ataatgaata
     1021 tgtaagaaag gaggagcgta aatgtcagtt tatacatgta ctttaaactt ggcaatcgac
     1081 ctttttatcg aaacagatga aatgaaacca tcccaagtca atcgaacaaa cgatgatgat
     1141 atacaagcta atggaaaagg tgtgaatgtt tcattgatca tgaagcaatt aggtatcgat
     1201 agtacagctt taggatttaa agcaggtttt accgggaatt atattgagga ttatttgcat
     1261 caaaaagata ttagcactga ttttattagc gtaccgggaa tgactagaat caacgttttt
     1321 acacaagtta ataaaaccaa tgaagaattc aaattggtta ataaaggacc tgagattcct
     1381 caggaacaaa tcaaaacttt tctaaatcaa atacaaaagc tgcaagctga cgattatttg
     1441 tgtgtttctg gcagtttgcc gcgaggggtc tcacccgaaa ttcttattga tatcagtcgt
     1501 atttgttttg aaaataaggt ccatctgatt attgattcaa gctatccgga aataattgat
     1561 tgtctgccgt atcagccgtt cttattaaaa ccaaacgagg aggaattgca atcttggtat
     1621 aatgtcgagc ttagtgatcg tgaaggatat attcattatg ctgaacgctt gattgccgat
     1681 ggagcgaaaa atgttctcct ttctctagga ggagagggag gaatattttt aaataaggat
     1741 atcgtttatt ctgggaattc tcctaaaggt aaagtagtca atacagcgtg tgctggtgac
     1801 actttgttgg gaacatttct tgcaggatat ttaaaaggtg aacaggtaga tattaatttg
     1861 aagaaaagtt tagcagctgg aagttcaacc gcttttcgta aaggattaac agatttttcg
     1921 gatgttgcag agttggaaaa acaaatcgaa atcaaagagg gagcgagtaa aaaatgacaa
     1981 cttacaaaat tgttgccgca acaggatgtc caacaggaat tgcccacact tatatggctc
     2041 aagaagcatt ggagcaggca gctaagcgca aaggggtatc tatcaaagtt gaaacccatg
     2101 gtcaagtagg gatagagaat gaattaacac cagaagaaat aaaaaacgct gacgctgtaa
     2161 tcattgcagc tgacaaagat gttcaagcag aacgtttcgc aggtaagcgt gtaatcgatg
     2221 tgcctgtcag caaggggatt aaagatgctg accaattgat cgaagatgcg ttggctggta
     2281 aaggttcaat cactgcagaa aacaaaacag ttgacgctgc tggtgagaaa gcggagatgg
     2341 gggttgcagc aggcattggt cacagtattt ataaaaatct catgaacggt gtttctcata
     2401 tgctgccgtt tgtagtggca ggtggggtaa ttattgcaat ttcgtttgcc atctggggtg
     2461 tgtactcttt tgatcccaat gatcctactt acaatccgac tgccgctatg ttaaagggtg
     2521 ttggtgatgc ggcaatggga atgatggttc ctattttagc agcttatatc gctgaaggaa
     2581 tcgctaaacg tcctggtttg gttgttgggt ttgttggcgg actgttagcc aattctggcg
     2641 gagccggttt cttaggcgga atttttgcag gtttcttagc aggctacttt atcttactat
     2701 tgcaaaaggt catgaaaggc ttgcctaaac aattggatgg tctgaaggcg atcttcttat
     2761 atccggtgat cggtgtcttg tttgttggtg tagtcatgtc tttattagca aatccaatgg
     2821 aagcagtcaa ccaaggaatg atgaatttct tggcaggatt ccaaaattct aatccattaa
     2881 ttttagggat tatcgttggc tgtatgtcag catttgatat gggaggtcca gtcaataaag
     2941 cggcttatgt tacagggact gcgttgttgg cacaaggtaa ccatacattt atggcagggg
     3001 tttcggcagc ttgtatcgcg cccccattga ttactggatt tgccgcactg ttttttggaa
     3061 aatatttcga cacgaatgat cgcaacgctg gtttagtcaa ctttatcttg ggctcaacgc
     3121 acattaccga aggagcaatt ccatttgcgg ctaaagatcc gctgcgtgtt ctgccaacta
     3181 tgatgctagg ttcatcaatt gctgctgtac tgacatatat gtttggtgtc caagttcccg
     3241 cacctcatgg aggttttcta gtattacctg ttgttactgg taaggttgca tgggtagggt
     3301 cgattttact tggttcaatc gttggcggag taattttagg gttgatccaa aaacgtagtg
     3361 tagaaaaacg tgaagcagaa gcagctgctg ttgaaatgaa agaagcaacc aacaactaaa
     3421 ggttattcta gaaagaagga tttcaaatga ctttgatcaa aacaaataaa gtttttatga
     3481 ataaaaattt tacgacgcaa gatgaggtaa tgaacttcct tgcagatcag tcaattgtct
     3541 tgagcattag tgaagaccga gatgcagttt acgaaaaatt gatggaaaga gaagaagaaa
     3601 gcacgactgg tatgatggat ggttttgcca tccctcatgc caaagatgct agtatcaagg
     3661 atgccgatat tgtgattgtt cggttaaacg gtaaggtcga ttggaaaagt ttagatggag
     3721 aaggaacgga ttttgtaatc gctctgttta ttccagattc agaggctgga acaacccatc
     3781 taaaattttt atctcaggta gccagacttt tgatgcacaa agatgtgaca gaaggtctga
     3841 aacaagccca gtctgctgaa gaaatagctg aacttttaaa ccaaaaatta gaagaaaact
     3901 aaaaaggaag tagtgaaatc atgcctttaa cagaaaaaaa gaaaacttat attcgtcaac
     3961 taagtaatga aagtggcatc atcagtgcgt tagcaatcga tcaacgtggt gccttgaaaa
     4021 aaatgatcgg taaatttcaa aataaatcag caaccgatga acaaatcatc aatttcaaaa
     4081 aaatcatttc ttctgaattg actccatatg cttcagcaat cttattagat ccagaatatg
     4141 ggttgccggc agccgaagtt cgcgataaaa atgctggtct gttgttggcc tatgaaaaaa
     4201 ctggttacga tgcttcaact ccaggacgtt taccggatat tttatctatt tggtctgtaa
     4261 aacgtctaaa ggagcaaggg gccgatgctt gtaagttctt gttatactac gatgtagatg
     4321 aatctgatga catcaatgac caaaagaagg cgtttatgga gcggattggt tctgaatgtg
     4381 ttgctgaaga tttgccattt ttcttagaat tggtttctta tgatgccaac gatcctgata
     4441 cttcttcgaa ggaatatgcc ttgaaaaaac cgcataaagt aaatgaaatg atgaaggaat
     4501 tttctaagcc tcgctatgga gttgacgttt tgaaagtgga agttcctgtt aatatgaact
     4561 atgtggatgg ttacactaaa aatgaagttg cttataccaa agaagaagct gctgcttatt
     4621 tcaaagaaca atccgagact accaacctgc catttatttt cttgagcgca ggagtaactg
     4681 ctgaattgtt ccaagaaacc ttgaaatttg caaaagaggc tggctctaca ttcaatgggg
     4741 tactctgtgg gcgcgcaact tgggcgaatg gtgtagaacc attcgttact aaaggtgaat
     4801 atgctgccaa agaatggatg cagacacaag gacgcaagaa catcgaagag ctgaataatg
     4861 ttttgaaaga aacagcaaca gctattaaat tgtaagaaag ctttaactat aaccttaagt
     4921 ttgtaaccta atgattttta ataatcaatt tgagctttta aataaagaat tggaagcgtc
     4981 ccgacgcttc ctttttcgtt tgtttttaag gcatgatagg ttctatgttg tcgttcaata
     5041 agcgcaagaa ggtcagcatt tttttcttag agtatgcaaa attgcttttc tacccaatga
     5101 tttaaaacgg agaggcaccc ttggatatgg tagatggtga agtaacgcat ttcttctgtg
     5161 atcacggttt ttttgtcttc ttgtagccag atttttaaat aattttcttc gatcagatta
     5221 tttaatctct tttgaaattg gatatctccg tggttgatga cgaataactt gtatacgaac
     5281 gtattcgtat agacatagtc gatcaattga gcgtatagat cctcgtagct atgtgtcgta
     5341 tcgtaggaaa tgatttcatc caagtcttgg aaaaagctat tttccagttt ttcataaaga
     5401 tcatagatat ccgtataatg tgtataaaat gtcgaacggt gaagatcagc agtttcggtc
     5461 agttcacgga tggtgatgtt ttgaattttt ttatctacta ataattttga taatgcttct
     5521 gctaacgctt tttctgtttt tttgacgcgg cgatcctctt ttttcatttt gcagcctcct
     5581 aatcaacata atcaaggaat tgtgtcggtt atccgacaat ttcaccgcta tctgaacata
     5641 gataaccgac accttgtcta ttataatata agtatagcat atcaatattt aaagaatagg
     5701 agaataagtg atggaattag cctttaagca actggaaaag aaagactaca aaaaagccat
     5761 tcaattcgcg attaaaggta tgcactttga ttggtatacc aagaacaaac tccttttgaa
     5821 tctttatgga agatactttt ggtatctaga attgaatcgc gcgacacaaa tcattgcttt
     5881 atatgcggat gatgagttgg caggtgtttt attagcagac atcaaaggaa aatcaaaaaa
     5941 gcatcattca tttagtcaaa agttatatgt taaactattt gatttcttac aaaatgcatt
     6001 tgtgaaagac agcaaaggcg ggtatgacga caccaatgac gaattgcttt cagactattt
     6061 aaaaaaacat tccccagatg gagaaattgt ctttttagct gccaaccctg atttaaagat
     6121 caaggggatc ggtagtaaat tattaaaaga atttgaacgc cgagagcaag gaaaagaagt
     6181 atttctgttc acggatagcg gctgtacgta tcaattttat gaacgacgcg gtttcgaacg
     6241 gaaaaaagaa aaagataacg tcatgaagat ccttaataaa gaagtagatc tccgctgtat
     6301 gttatatagt aagatcattc agtaatgttt tttactatca gaaagcagaa gtaccacgaa
     6361 ggtcaatgga aaaagcgagt aattgatcca tttagatgaa gataggtgta gtctcaaaga
     6421 tattagctgg caaagatatc cccagttaaa aatagatttc tgtatccaac aataaaaata
     6481 tttttgtatt tattagtata tatactttac aaaaatttgt atatatacta atttttttgt
     6541 aggatcaatg aaatgagggg aaaatatgcc aaaatataaa gaaatagcct tggctctaaa
     6601 agagaggatc gttgagggaa aatatcagga aggttctaaa ttacctgagc aagaaagtct
     6661 ggctagagag tttaaaacaa gccgtgttac tatccgcaaa gctatacaac tccttattga
     6721 tgaaggtttg ttatatacga gacgtggatc tggtactttt atacgaagca atatcagaca
     6781 ggacaatgag aatgtcactc aagtaaataa tgtatttggc acttcctcac aagaaaatac
     6841 aaatgtgaca agtaaaataa tccgttttga tgtccggttt ccgacagatt atgaaaggcg
     6901 gctttctgca acctacttcc gatccccttc ctgcaaatca tgggtataaa aaataggcat
     6961 attaagcggg ctaggaggag aaatattcac atatcatttt ttcattccgc aagctgcgtc
     7021 aagttatttg gaatttgtag aacagatgag agaggtagaa gcagcactac aaactacttt
     7081 tcaataagta ggaattatag aaattttact caaaaaatct tcattaaaaa atctgactaa
     7141 ataagttgag aaaacagaat atacttcaat atccagtgat ttataaatat cattcactca
     7201 tgatacccag ctccgtatca taagtgatgc ggtttttatc attgatccta aaaatcttag
     7261 caagacaaaa agtacgtaag aaagtaaaga ctaaatactt cggaggaaca aatgaataac
     7321 aatgataggt tattgcgatt gcgttatgcg attgatttga aagatacgga tttgattaaa
     7381 gcttttgagc taggcggtgt accactgact aaagaagaag ctcaggcggt actgaccaaa
     7441 gtacaggata agaacaaaga taatgcagaa aataatgtgt atgaaaaaac catcaacaac
     7501 caagtttttg acgcattcct aaatggcctg attctcttgg cgcgtggtcc gcaaaaagat
     7561 aagccgatag tagacacgcc gcaaaaaagc aaggaaatca aatatatcaa caatgtgttg
     7621 ctaaaaaaat taaaaatcgc cttgtctatg accactgatg atattttaga agtttttgct
     7681 gaggcagaac tctatccatc aaaaggcgaa atcggggcat ttttgagaaa agaaggccaa
     7741 cgaaatttta agccttgcgg agataaatat atgcgtaatt ttctcaaggg gttaggaatt
     7801 tataatcgga gaaaagtata aagaaatttc atgagaaatc aaaacaatgg ttgttttgat
     7861 gagagctagt ttttgtatga gattaaaata ttatcttaat cgaggagctg gattggtttt
     7921 atgaatgatg aatataagaa tgatgaagat aagatgcttt ttgaagagat agagaatcga
     7981 tgtaggctca attttgagct tcggggaaag atgtctttaa tacaacaaaa aaagtattta
     8041 gcgaataaat cagagtttac tctgggacat gttgaaaaat taatctcgga ttggattagt
     8101 agtagaagcg aatttacaaa aataaaacag ccgattaaat ttgacatgaa aaaacttttg
     8161 ttaaataaga gtgagatagg taatagagat caatatatta gagctaaagg gcaagaaata
     8221 attgattcat taggtgaagt gaggtcatac aattatttgt atgtaacaca tagagctgat
     8281 ggaatggtca ttactgtggg gaaatcttct tctaatgata tttttttaga tggagatctt
     8341 ttttatcaac tcaatacaaa tcacttgtct ggcactgaaa atataatttt aaggacggaa
     8401 tatggcaatg aaatatttgc aaaatatgat gagattttaa aaaattattt ggactgggct
     8461 tggataattc cggttgagtc aggagatgct aagaaactgg agaga
//
LOCUS       DAXWOO010000120         8505 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.122, whole genome shotgun sequence.
ACCESSION   DAXWOO010000120 DAXWOO010000000
VERSION     DAXWOO010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8505
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.122"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(194..871)
                     /gene="nth"
                     /locus_tag="ACGE70_002118"
     CDS             complement(194..871)
                     /gene="nth"
                     /locus_tag="ACGE70_002118"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFF8693739.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(878..1576)
                     /locus_tag="ACGE70_002119"
     CDS             complement(878..1576)
                     /locus_tag="ACGE70_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFF8693740.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            1844..3028
                     /locus_tag="ACGE70_002120"
     CDS             1844..3028
                     /locus_tag="ACGE70_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFF8693741.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3034..3600)
                     /locus_tag="ACGE70_002121"
     CDS             complement(3034..3600)
                     /locus_tag="ACGE70_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFF8693742.1"
                     /translation="MTSFVNPFFSWFQIGFSYKIFLSLLNRKQKNNIMKEKEGVEMDK
                     RQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHIGKIRDPNVTKLANASYM
                     TRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKIRNRHEQLHQKWVQRDAT
                     VFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(3703..3990)
                     /locus_tag="ACGE70_002122"
     CDS             complement(3703..3990)
                     /locus_tag="ACGE70_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFF8693743.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4161..5513)
                     /locus_tag="ACGE70_002123"
     CDS             complement(4161..5513)
                     /locus_tag="ACGE70_002123"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFF8693744.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(5786..6232)
                     /locus_tag="ACGE70_002124"
     CDS             complement(5786..6232)
                     /locus_tag="ACGE70_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFF8693745.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(6468..7394)
                     /gene="cysK"
                     /locus_tag="ACGE70_002125"
     CDS             complement(6468..7394)
                     /gene="cysK"
                     /locus_tag="ACGE70_002125"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFF8693746.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(7534..8421)
                     /locus_tag="ACGE70_002126"
     CDS             complement(7534..8421)
                     /locus_tag="ACGE70_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFF8693747.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT         2313 a         1750 c         1410 g         3032 t
ORIGIN      
        1 ttattttcta catcgggtat tttcccaata aaaataacaa cttttatttt tatcgcaata
       61 aattgtaata agttaccatc ctagctactg agtatgtaca tttacttttt catgcaaaaa
      121 agataaacga aacagctttt ctccataata acaagataat tcatacattt atttgcaatt
      181 aaagaaacca cgtctacagg cgtggtttct tttttagcgc cttttctttt agacgcatcc
      241 gatttttccc atcttgacat attgatagca aaggacatac ctcgcacttg ggatttcttg
      301 ccgtgcaatg atatctacca aaaaaaatca gtgtatgatg tgtctttacc cataattctt
      361 gtggtacttt ccgcatcagt gtttcttcta cttccatgac cgtagcatct agtttgcaga
      421 tcctcaggcg ttttgagaca cgttccacgt gggtatctac tgcgattgct ggtatcccga
      481 atgcatcccc caatacgaca tttgctgtct ttctgcctac accaggaaga gacatcaatt
      541 cttctcttga cgttggcact tgcccatcga aacgttcaat cagctgctgt gcacacgctt
      601 tgatgttttt tgctttattt cgatacaaac caatcgtttt tattttaaga atgatttcat
      661 cgattgaagc ttctgctaac gcatcgggtg tcggaaaaga ggcaaacaaa tctggcgttg
      721 ctttattcac tgatacatct gtcgcctggg cacttaagat cactgcaatc aataactcaa
      781 atggattatt gtgcttgagt tctccatgtg cttcgggaaa cattccatac atcgtttcta
      841 gagcttccat tgttttttgt ttgcttaaca tattccgcta ctcctccaac caatttttca
      901 gtgagacttt cggcaattct tcttgttgag ctgactgttc tacttctctt tgaagaagct
      961 gctgttttct tcgtttttgg tcttcttcca cttgttgttt tgtacgtaga ttttttcttt
     1021 cccacgatag caaaatgcgg tcaacgtaat taaaactata agcctgattt aatacggctt
     1081 cccgcagtgc tagcttcaaa atttccggct gataatggtc ttcttctagc cattgtccga
     1141 tccgctggaa ctcgatcgaa gaaagcgggc gtccaaattc ttgttcaaat aattgataca
     1201 ctgactggat ttctttttct tgtgtttttt cttcgtattt tttttcttgt gtttttaaat
     1261 actcacctaa tagatcatag atggggtaaa gattatatcg atctgctttt ttaccgttga
     1321 caatcgtcgt ttctatcttg ataaatccat ttgttacgag acgatccagt atttgaaaaa
     1381 tctgatcttg tttgattccc atatccatag caatcatctg caaatcaggg aaagtatctc
     1441 cttcaagctg tgccatatgc aactgtagaa caaataaaaa ttcttcagat tgtagaccga
     1501 tcctatgata atttttaagt aaaagattag atatgactgt ttgtccagcg cttaagtaat
     1561 tttctaaatt caacatgatt tctcctccgc tcaatcacag tatacgtaaa aaaaacatgg
     1621 gaagcaagaa caggtttctt ttctatttac ttcactgatc ggtcagattt acaatgtcgt
     1681 ctacttgatt tttgtttgtt catgtttttg atgcgtatct tcacttttcg gaataaataa
     1741 attaagtaga tttcgctgtt ccttagaatg ttgacaagga aacaaaatta ctatacacta
     1801 tttttgtttc caagttaaca ttattgcata aaggagtttt tacatgtaca gtttcacaaa
     1861 taaacattct aaagtttttg gatacgtctc tgtcttattc actggattag gatttactat
     1921 cgtcagtcct gtacttccgt ttttaacgct gccttattct cactccatac atcagcaagc
     1981 tttctatatc actttgctca tgtctgttta tgctctagct gccttcttat ctgcccccat
     2041 tctcggtagc ttaagtgatc attttgggcg tcatccgatc cttattatca gtttattagg
     2101 atcaagtatc ggatatttgc tttttggttt aggaaattcg atatggatgt tgttccttgg
     2161 tcgtatcatt gaaggattga cggcaggaga aatctccacc ttatatgctt actttgccga
     2221 catcacggaa cctaatgaaa gaaccaaagt ttttggatgg atgggtgctt tagtcggtat
     2281 aggaacaacg ctgggtccta ttataggggg tctattagct gagttgggga attctgttcc
     2341 tatatttatt ggagcactct ttactttctt aaatgccgtt tatggctaca catttatgct
     2401 agaaagcctt ccaataaaaa aacgttcagt agatttaagt ttctcccatg taagaccttt
     2461 tcaccaatta caacaattat tcaagatttc atccgttatt cctttattga cagcaggttt
     2521 tgctgtttgg ttggcagctg gctctcttca atctatcttt tctcaatttt cgattgacgc
     2581 ctttcagtgg aaggctgggt tggttggttt aagcttctct ttgattggaa tcttggatac
     2641 tgtctcgcaa ttgttcatta tgcctcgatt gctcaaaaaa ttttcagaac agcagatcac
     2701 aagaataggg atgttcagtg aaatcctcgc ctatctattc atcacattat ctggaattct
     2761 tctactgccg attttgtttt tatttgggat tatctgttat ggtttcggtg attctatttt
     2821 tactcctgta ttcaatggcc aactatccaa ttctgtctca gataaccagc aaggtcttgt
     2881 aatgggcggt acccaaagta tacaatctct atcgcgcgtc atcggcccgc taatcgcagg
     2941 acagctttat gctgcagcac catttcttcc gacggtattc ggatttttgc ttatgatttt
     3001 tgcctattat cactacaata aaaaatagta gcttcatttg ctacttcgta taaattctct
     3061 gtatcgaccg ataaaacgaa atactgtgtc aaattcttct tttgacatat tttcaaatac
     3121 agtagcatct cgctgtaccc atttttgatg gagttgctcg tgtctattcc ttattttttg
     3181 tccgattggc gtcaattgaa aatagatttc ttttttattc tcttcgcttt gataagtgtt
     3241 tatgatccct ctagctatca gtttttttgt tagcttactg atggctcctc tagtcatgta
     3301 actagcattc gctaattttg tcacgttagg atcacggatt ttcccgatat gttcaatcac
     3361 ttctagttca tttagagtaa atcctgcaaa ttctttttcc atttctggtt tattacgatg
     3421 aataatctca ttagctaaat ccagatattc ttctattagc agttgtcttt tatccatttc
     3481 tacaccttct ttttctttca ttatattgtt tttttgtttc ctattcaaca aagataaaaa
     3541 tattttgtag gaaaacccaa tctgaaacca agaaaaaaag gggttgacaa agcttgtcat
     3601 aacccctttt tctgattaaa agatagtaaa gagacagtca tcggtaattg tgatgatttt
     3661 ttgtcatcac ttaaggatga gcggcctttt caaaaactgt ctttaaagat catcatcagt
     3721 ttctagcaca tatggctgac cttcatatgt gtactctgta ttcaatgtgc cgtcattttc
     3781 atctgagtag atgcgtctac tgaaaggaaa acgatgagaa acattatctt tggcgtcatt
     3841 tgctggcatc gtgtaatatt cctcttgcgt ttcattcaac tgagagagta cttgatctgc
     3901 caactcatca atattgatcg ttaatttctt cgaagcactt ttataatctt ctaaaaatgc
     3961 atcttgttgg acaaaatatt ctttattcat cttctttctc acctttcttt tcaaaatcaa
     4021 ataaagctgc ttgttctttt atgtaacagc tttatctttg ctgcttattg tgacaaaaaa
     4081 ttcgaagaaa gacaactaat tgtacttttc acaaaaaaag acagagggtc gtgacaaaat
     4141 tgtgtcacaa ccctttttct ttattgtgca gtttcgattt cgcctagcgc tgcttgtgca
     4201 agtaggttca agtaattcca tgggcggtca aaatgtggtt ggaagaagaa atctgaaagt
     4261 gcgagatctt ctacagtcat tttattttga acagctaaag acatggtatt tgctgattgt
     4321 gtaatgtcgt atttggacat gaactgagca cctacgatac gattcgttcc tttttcatag
     4381 actagctcca tcattacttt ttcagtagtt ggcatgaact ctggacgata attatcttca
     4441 aatacagtcg cttggacttc tagatcgttc aaagttgcgc tttctttggt cacacctgtc
     4501 gaaccgatcg tccagccaaa taggtaaagt cctgaagttc cttgagtacc acgatacgcc
     4561 attttatgat ctgtcaagtt atttcccaca agcaaacctt gacgaacggc atttgttgcc
     4621 aaaggaatat agttttgcgt gccacttgga ttgtagttga ctactgcaga gtctccagct
     4681 gcaaaaatgt ctggattact tgtctgcatg tattcattca ctttgattgc cccgtttggc
     4741 agtgtatcta cttttccttc gatcagtttt gtatttggac ggaagcccac acataaaatg
     4801 accatatctg tatcaaatgt atctgttggt gtagctactt gtttcacttt tccttcttca
     4861 tctgtgataa attcagtaac attttcgcct agcgcaagag taacaccgcg gtctgtcaat
     4921 tctttttcta agatatctgt gaatggttta tctagatatt tattcaagat gcgatctaat
     4981 ccatcaatca atgtcacttg ttttccagat tcgacaaaag cttctactaa ttcaataccg
     5041 atatatccac caccaacaat tactacacgt tttgcttctt tcgcttgtgc aataatttca
     5101 tttgcttgat tgtagttttt gcataggagt acatttttcg catcgattcc actaatcggt
     5161 gggataatag gccatgaacc agttgtcatc actaatttat cataagaaac cgtttctgtt
     5221 tctcctgttt tcagatcttt tgccgttact gttttatttt ctgtatcgac gtctgttaca
     5281 tcgtgttcca ttttaactgt tgctcctaat gaagataatt cttcaggatt tgagtagaac
     5341 aagcctgctg ggtcctttac tactccgccg acataaagtg cgataccgca agataagaaa
     5401 gaaatattgt catttctttc atatactgtc acttctgctt ctggatgatt ttttaaaata
     5461 ctttttacag ctgcagtacc tgcatgagta cagccaatta ctactacttt catttatggt
     5521 ccctccgatt aacattttgt ccttcgacaa aactactata acagaaataa aacggataca
     5581 acagtttaat gcttgtgaat tcacaaaggt ttgtttgtag tttcattcct tttcaagcat
     5641 aagaacccaa tatctacaag attcttattt taataatatt cacttttata tctaagattg
     5701 tgaataaaag acaaaaaaat aaaaaagcta gcgttttttt tacacagcta gcttttttta
     5761 ggtataacac ttgtagaaac tgtttttaca tttgcgcctt ttttgctgac tctttacaat
     5821 ccggacagag tccatacaat tccaaccgat gttcattaat ctcaaaatca gtcagtctgc
     5881 ttgcagccat ttctatatct tcaagccctg ggtaatgaaa atcaacaatc ttaccgcagt
     5941 tttcacaaat tacatgataa tgacgctttg aactgaaatc aaatcggcta gaagcatcgc
     6001 cgtacttcat ttcttgaaca aatccaatcg aagtaaataa gcgaaggtta ttgtaaacag
     6061 tcgcaacact catatttgga aaccgatgtt caagtgcttt ataaatctca tcagccgttg
     6121 gatgggaatg atgttcaatc aaatattcta aaaccgcgta ccgttgaggt gtgattcgaa
     6181 tgttctcttt ttttaacttt tcgataacct cttcgactaa cttattttcc atgtaaacac
     6241 cccacgttgt tattctcgtc ttatcatact atttttacat tttaaaatcc acttaacatg
     6301 aaaagaaaat tcaattttat tgtattttaa tgaaaaaaga cgtaaaacag tcgcgttcac
     6361 tattctacgt ctctattcaa ataatttttc tcatcataaa tttttgtgtg ttcttttcat
     6421 ctttaaaagt tttttacata aacctatctg gagaaaaacc aagtgcctta tatctcttgg
     6481 taaagggctg tagataaata acgttcacca ttgtcgggaa ccacagctaa taccttcttg
     6541 ccttttccta attcttttgc gacttttagc gcggcagcaa ttgcagcgcc agatgagatg
     6601 cctactaaaa tcccttcttt tcgcccaact tctctagctg tttccatcgc ttcgtctcca
     6661 ctaatagaaa ggactttatc atacacagaa gtatccagtg ttttaggaac aaaacctgta
     6721 ccgattcctt ggattttgtg aggtcccgct tctttccctt ctaaaatagc agattctgct
     6781 ggttctaccc cgattaattc gattttcgga tacacacgtt ttaattctcg gccagcgcca
     6841 gtgatcgttc ctcctgtacc gatacctgca acaaacgcgt ccaagccatt tactccaaat
     6901 gcttgatgta tctcaggacc agtcgtcttc tcatgcacaa gaggatttgc ttcattttca
     6961 aattgtaatg gtaaaaagta gccgttttct tttgccaaac gttctgcttc tctgattgat
     7021 ccagaaatac catccgcacc aggagtcaaa atcaactctg cgccgtaccc tttcatcaat
     7081 aaacgtcttt cgatactcat cgtctcaggc atcacgatga ttactttata tccttttgca
     7141 actcctacca tcgacaatcc gattcctgta tttccagaag taggttcaat gatagtatca
     7201 cctggtttca gtagaccatc gtgttctgct ttttctatca tacttaaagc aatacgatct
     7261 tttacacttc ctcctggatt aaaaaactca agctttacaa aaacatctgc tgaatcttct
     7321 ggtacgattt tatttaattt gacgattggc gttttgccaa tcaattcggt gactgagtta
     7381 tagatagctg tcatcttcaa ttcctccttg tctttgctct tcttacttat cataatacaa
     7441 tctgttcatt tatggcgagt gattcgtctt tgactagcaa aaaaaagaca aagatatacc
     7501 atatatggta atttctttgc ctttttatct aaatcaaacg tttagttctt gaccaactaa
     7561 taaagaataa ctggacatag acatgccatt tttcgccatt agatcatcaa cagacatgcc
     7621 aaatttttgt gcaatatccc atagcgtatc acctgacacg actgtatatg tctgaccatt
     7681 atttgtattt acatctgttg ttgaagatga tgacgttgaa gtcgtcgtag tagtcgttgt
     7741 tgttgtactt gtggttgttg tagccgaacc tgttgatggc gtatcaaaac gggttaaatt
     7801 ataagtatta atcaatgagt tcaatttggc cccataattt ggatcggtag cataacgacc
     7861 tgtcaagtac gctgttgcat cataaaaact tgttgtattg cttttccaca cgccagcata
     7921 atgataatcg cccgtggaaa aactggttga acgaagaaca ttcgcatggt cttggaagga
     7981 ttcccaataa gaagaatatt ttcggaatgg ctcattcatc gttacccatt gtccgtttac
     8041 atattctttg gttgccatat aaacgacctg tccgttatag cttcctttca caccgaatag
     8101 attataattt ggcgcactag ctaatccgga agtgccatat gcactttcca acaacgcttg
     8161 agcgatcatg actgaagcat acaagtcatt acttgcagca acttctgacg cagaccagcc
     8221 gatttgatcg ataaacgctt gaggatcagc gactgtttga gatccttgtt catctgcatg
     8281 aacatgttgc ggtaccgctg taaccatcat cggcccaact aaaagactcg ttccaattaa
     8341 aggagcaatc gcttttttat tgatcaccat ttttctttta ggtctatggt gccgggcact
     8401 gcgggtcttc agctcttgca ttcaaattcc tcctcaaaat aaaaaaactt aataaaaagt
     8461 tctcttctta aaacaaactt aattaattaa attatacttt taatt
//
LOCUS       DAXWOO010000121         8484 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.123, whole genome shotgun sequence.
ACCESSION   DAXWOO010000121 DAXWOO010000000
VERSION     DAXWOO010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.123"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..208
                     /locus_tag="ACGE70_002127"
     CDS             <1..208
                     /locus_tag="ACGE70_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693748.1"
                     /translation="DVYDLLIHKNENSNIGRIIITNLIVITIGVTTLTNKALISSMIL
                     IVFDFIIIYSLLFLIKKVTEYLNR"
     gene            375..938
                     /locus_tag="ACGE70_002128"
     CDS             375..938
                     /locus_tag="ACGE70_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693749.1"
                     /translation="MTAIYKALKNSNVSEYVKSRVLNNNLDYILENGVADLISKLSDP
                     IDIDHLVVWQDGTLEVSIYGKFYSRNKVTTFKNSISGREDLKGINKNSKFYRRNKELL
                     EKISKRVSELVNPFSKKRSKELFGIFYDGFSAKSNVEKFFYFNDSAYVAVKDSKRIPE
                     ATLEYLEPIKLSVFYSLYEKEIADEAV"
     gene            949..1809
                     /locus_tag="ACGE70_002129"
     CDS             949..1809
                     /locus_tag="ACGE70_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraA"
                     /protein_id="HFF8693750.1"
                     /translation="MSKKIGKESLSLGLKKEIVKFKSMYREPEFRLIFQLDIVRGLKI
                     SFSCLGYLVWLFIARVGWSNAYSFYKLFAGTDRLKESLEVKEALNILGSLAFSLFIFA
                     LFILQVGKIMMSTNDNYINYLKPFQKGIKIICRQGTVLFLLSLYEHEIHFQNYYNTTT
                     KLGAFLLLYLVSNIAEKMLFKHIVKQVFSDIQKESYCLKTPYGNLKNVELFLFKMDSK
                     WDKYIYRSKEVFLTGSYNETLQESLYDTGAIFNNEPMVLKDFFGDYELVWNPKQLSEQ
                     GAEDEFAEVH"
     gene            2307..2561
                     /locus_tag="ACGE70_002130"
     CDS             2307..2561
                     /locus_tag="ACGE70_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693751.1"
                     /translation="MYNVGDQIDEIVKIGNVKLVKSKYYISETEIIFNTESGCILIWK
                     LPFPKDETTLKKINTLKIRYCIHEIIDKNTYVINQVKVMG"
     gene            2959..4560
                     /locus_tag="ACGE70_002131"
     CDS             2959..4560
                     /locus_tag="ACGE70_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFF8693752.1"
                     /translation="MLNSCEYADVLAKILKNGIKQFNSKNLVAFKKNSNKIGAIAGFT
                     SKTNMIKTKGLIYRSIDRVLEDSDVLTHWTPNVYSWLGGGENKTIFGHKEANLIQINT
                     FVADIDYPVGEKKTDLNLLVLYLLEEGLLPTLIVDTPKGYHCYFLIQNYNQETDSFDK
                     ASYISNSNDFKSLRVAKRISENIRKSIQNRLPQVDMGCNHFGIFRFPTKKNIVHYEPN
                     FVDTFEGYLKWSKDFEEKEYALKRSNLSLLAPNSTGKKGSKRQIDTKWYDFLIHTDIA
                     EGDRNKTIFTLALACKSSGLSIGECMDQLDQLQFNNDLSYSEVERTIRSAYRGDYKGA
                     KSSYVNEIIENYATPTEFEKYSINVNRQAVKRKGNRVDPSNWVKFAKPREERKYSHFE
                     ESKADLLAYLERKQRSLKKNELFLSVSMGTISEETQIPVSTLKVILKELRTANTLILQ
                     TSRGRYGSTKIATSKFVEAKILSTVIQNKKDKQVFVLELLGTANSHVETILRRYEDKT
                     VPFHANILKEREKSKHAPIRGANTG"
     gene            4794..5093
                     /locus_tag="ACGE70_002132"
     CDS             4794..5093
                     /locus_tag="ACGE70_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFF8693753.1"
                     /translation="MVIKKYVYSHPINVFIIGKLGLSVEVFCELYGFKQGTLSSWITR
                     EREVADLPISFLYALSLASGGNMNEVYDCLCEYEKEYKEYLKISAPKRNKKYIED"
     gene            5228..5605
                     /locus_tag="ACGE70_002133"
     CDS             5228..5605
                     /locus_tag="ACGE70_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HFF8693754.1"
                     /translation="MNIDGIYNESDFKKGIVDIDRKEYLFPDEIGLFKIVGETVCRYW
                     GKKGNIILLVDTIEGRKLKFSFWSNTKYCCEPVPKREVDVAKESVRFLPDRSKITLTC
                     ERTSNAKFTRIRDILLSTTSSQS"
     gene            5654..8017
                     /locus_tag="ACGE70_002134"
     CDS             5654..8017
                     /locus_tag="ACGE70_002134"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HFF8693755.1"
                     /translation="MAVVILAEKPDQGAKFASALAGKKLSKKSGKYEFKSEIFGDTIV
                     TWGIGHLVGLSLPEKYEWLPNKDKWHLDNLPFLPKFNELKYEVSKGKNQQFSIVKSCL
                     ENADEIIIATDPDREGENIAYNIFKLCKQSIFNKPIKRLWINSLVQKEIIKGFKNLRN
                     SEETISYFKEANARQIADYLVGMNYTELYSLKLQEKGISGVVSLGRVQTPVNTLVVEN
                     DLAIRNFRPEKYKTIECHTTDKQPKAIFKNSLEYFKLEKFEEDTKKFGLDSASTGIIK
                     KIEVSKKQTEPQKLFSLGGIQEYANKKWKYSAKKTDKIIQSLYDRGYLSYPRTDSELI
                     TTNEFEYLKANLDVYKSVLGISIDTPCLEPKKRFVDNDKVLEHYALIPTMDIPDIANL
                     TLDEKNIYEIVTRRACLMFAEPYKYENTKVTLDVNGLEFTTSGNVPISLGWKQVDTNL
                     DESDDKEETLPKFHEQETINIDIRFIDKLTKEPKRLTEGKLLGKTGIMAKLGLGTPAT
                     RSNIVDTLLNREYIKIENTNVYPTPKGYLLWDLTKNKDLLIGKPENTAIWEESLGKIA
                     QGNYTQEAFLRNIYNYINKTVSDLKKVEFTSDYLEKTVNQNKFDIGSYQVEEKKKVYS
                     ILNINSGEQFVIFKNISGKAINKKVLQELLLNNRTDKVLSGFKSKEGKAFNARLLFDP
                     NVMKVVMEFEKNSPKGDKKSKHDTVNGYEIVEKAKVFEIKELATGESFIFYKNNSGKT
                     LSSKTVKELLENGKTKEKVTGFISKDNKKYSAYLVFDNKNKVIKKEF"
     gene            8257..>8484
                     /locus_tag="ACGE70_002135"
     CDS             8257..>8484
                     /locus_tag="ACGE70_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693756.1"
                     /translation="MRAISFKKDYRLLILAYIIHWKGGDPMNLKNTNSNKSTLYVISD
                     DYINKLKKVDYRVQNNYREQRLYFKSNLKISN"
BASE COUNT         3286 a          998 c         1537 g         2663 t
ORIGIN      
        1 tgatgtctat gatttactaa ttcataagaa tgaaaatagt aatattggta gaattataat
       61 cacaaactta atagtaatta ctattggggt tacaacatta acaaataaag ctttaatatc
      121 aagtatgatt ttaatagtgt ttgattttat tatcatttat agtttgctgt ttttaataaa
      181 gaaagtaact gaatatttaa atcgttaggc acgaaataaa ttttaattga ggttgaatta
      241 tagcggtaaa tcatacaaaa tagaaatgta gtcttgttaa gcgtcttggc tatctaattt
      301 tgaatgagga gggttgtata tagaaaaagg gtagttaaat tcgtagatca aaaggctacg
      361 aaggagaata aagaatgaca gctatttata aagcactaaa aaattctaac gtatcagagt
      421 acgttaagtc gagagtatta aataataatt tagattacat tttagaaaat ggtgttgctg
      481 atttaatcag taagttaagt gatcctatag atatagatca tttagtggta tggcaagatg
      541 gtactttaga agtatcaata tatggaaaat tttatagtag aaataaagta actacattca
      601 aaaatagtat tagtggtcga gaagatttaa aaggaatcaa taaaaatagt aaattctata
      661 gaagaaataa agagttgtta gaaaaaattt caaagcgtgt ttctgaactg gtgaatccat
      721 tttcaaaaaa acgtagtaaa gaactttttg gaatttttta tgatggtttt agtgcaaaaa
      781 gtaatgtaga aaaatttttt tattttaatg attcagctta tgttgcggtt aaagacagta
      841 aaagaatccc tgaagcaact ttagaatatt tagagccaat taaattgtct gttttttatt
      901 cactttatga gaaagaaatt gctgatgaag cggtttaagg ggaggaaaat gagtaaaaaa
      961 ataggcaaag agtcgttatc gctcggattg aaaaaagaaa ttgttaaatt taaaagtatg
     1021 tatagagagc cggaatttag actcattttt caattagata tagtgagagg gctgaaaatt
     1081 agttttagtt gtttaggtta tctggtctgg ctattcattg ctagagtagg atggagcaat
     1141 gcttatagct tttataaact gttcgccgga actgatcgat taaaagaatc attagaggtg
     1201 aaagaagcct taaatatttt aggaagtctt gctttcagcc tgtttatttt tgctttattt
     1261 atcttacaag taggaaaaat aatgatgtca acaaatgaca attatattaa ttatttgaag
     1321 ccttttcaaa aaggaataaa aatcatttgt agacagggca cagttttatt tctacttagc
     1381 ctgtatgagc atgaaattca ttttcaaaat tattataata cgacaactaa acttggtgca
     1441 tttttactcc tatatttagt tagtaatata gctgaaaaaa tgctgtttaa acatattgta
     1501 aagcaagtct tttctgatat tcaaaaagaa agctattgtt taaaaacacc ttatggaaat
     1561 ttaaaaaatg ttgagctgtt tttatttaaa atggatagta agtgggataa atatatctat
     1621 agaagtaaag aagtattttt aactggtagt tataacgaaa ctcttcagga gagcctgtac
     1681 gacacaggag caatatttaa taatgaacct atggttttga aagatttctt tggagattat
     1741 gaactagttt ggaatccaaa acagcttagt gaacaggggg cagaagatga atttgctgaa
     1801 gttcactaac atagtgtaca gaaaaacatt atctgaaaga agaacgcctt caaaagaaag
     1861 tataagaacg attgggccgt acagaagact aacgaaagat cgtaaaaaaa aggaaagaca
     1921 atgaaaatgt ctttcctttt ttttgactac aagtagcctg aaaatgaaat tccaaaagac
     1981 tacaagtagt cttttgtggc aaaaataaag agccaacgaa agctaccaac aatcgttgac
     2041 tcgtaagact gttcgtagta agaaaaatct ttggcaatcc acgaagtctt tatttcataa
     2101 tttatataaa tgattataca gtcattttcc tgaatttgca agtaaaaaag ctaagaaata
     2161 ttgtttaagc gtttccttaa aagaatcttt aaataaattt ttttccaatt gaaaacatga
     2221 ctacaagtag actatttctt ttattccaag ttgactactt gtagtcaagg tgatataata
     2281 tgtatatcaa gaaaggagaa acaaaaatgt ataacgtagg agatcaaata gatgagatcg
     2341 taaaaatagg taatgttaaa cttgttaaaa gtaagtacta tatttcagaa actgaaataa
     2401 tttttaatac tgaatcagga tgtattctaa tttggaaact gccttttccc aaagacgaaa
     2461 caacactgaa aaaaataaat actttaaaaa taagatattg cattcatgaa attattgata
     2521 aaaatactta tgttataaat caagtaaaag tgatggggta aaaatctttt aaaaaaaact
     2581 cttgttaaaa gtaattctat tagcttataa tgttggtatt agttctgaag ccaaaggaac
     2641 actaaaaaat tctagtagaa aagttagtta cagactctct actatgtatg aatgtaacga
     2701 aaaaaatagc gaaaaagata attaaataag actaccaatc ttatttaatg gaacttctgt
     2761 agtaagaaaa gcgatttggc aatccacgaa gtccaccaag atgagctacc aactcatttt
     2821 aatttttgca cgtttaaagt ttagcattta agtgcttact ctaaagacat agtgtaattt
     2881 taggactcct tattacggaa tctaaagtta ccctatgtct tttttgctat tcaaaattga
     2941 tagcaaggag aaagtgcaat gttgaatagt tgcgagtatg cggacgtttt agctaaaatt
     3001 cttaaaaatg gaattaaaca gtttaatagc aaaaatctag ttgcttttaa aaagaatagc
     3061 aataagattg gtgctattgc cggatttact tctaaaacaa acatgataaa gactaaaggt
     3121 cttatatatc gttctattga tcgtgtcttg gaagattcag atgtattaac acactggact
     3181 cctaacgtgt attcatggct tggtggcggt gaaaacaaaa caatttttgg tcacaaagaa
     3241 gctaacttaa ttcagattaa tacttttgta gcagatattg actatccagt aggagaaaag
     3301 aaaacggatt tgaatttact agttctttat ctattggaag aagggttatt gcctacactg
     3361 attgtagata cgccaaaagg ttatcattgc tattttctga ttcaaaacta taaccaagaa
     3421 actgattcat ttgataaggc aagctatatt tctaattcaa atgattttaa atctttgaga
     3481 gttgctaaaa gaatatcaga aaatatcaga aaatctatcc aaaatcgttt accacaagtc
     3541 gatatgggct gtaatcattt tggtattttt agatttccaa ctaaaaaaaa tattgtacat
     3601 tatgaaccta atttcgtaga tacgtttgaa gggtatttga aatggtcaaa agactttgaa
     3661 gaaaaagaat atgctttaaa gaggtctaac ttgtcattat tagctcctaa ttctactggc
     3721 aagaaagggt ctaaacgaca aattgatact aaatggtacg actttttaat tcatacggat
     3781 attgctgaag gagatagaaa taaaactatt tttaccttag ccctggcatg taaatcaagt
     3841 ggcttatcta ttggcgagtg tatggatcaa ttggatcaac ttcaattcaa taatgatttg
     3901 tcctattctg aagttgagag aacaataaga agtgcttatc gtggcgatta caaaggtgct
     3961 aaaagttcat atgtcaatga aattatcgaa aattatgcta cacctaccga gtttgaaaaa
     4021 tactcaataa atgtaaatag acaagctgtt aaacgtaaag ggaatcgtgt cgatccgtct
     4081 aattgggtga aatttgctaa acctagagaa gaacgaaaat atagtcattt tgaagaaagt
     4141 aaagctgatt tattagctta cttagaaaga aaacaaagat cattgaaaaa aaatgagttg
     4201 tttttaagtg tctctatggg aactatatct gaagaaacac aaattcctgt atctacgtta
     4261 aaggtgattt taaaagagct tagaacggcg aatacattga tattacaaac gtctcgtggt
     4321 cggtatggat cgactaaaat agcaacaagc aaatttgtgg aagctaaaat attaagtaca
     4381 gttattcaga ataaaaaaga taaacaagta tttgtcttag aattattagg aacagcaaac
     4441 agccacgtag aaacgatttt aagacgttat gaagacaaaa cagtaccgtt ccatgcgaac
     4501 atactgaaag aaagagaaaa gagcaaacat gcgccaatta gaggagctaa tacaggttag
     4561 agttctattt atttttttga tttttaaata gctaaattgc caattaacat atctagtgtt
     4621 tctgcccact cctattcctg ttttaacgta tactaatgat tagtagttag tactctcttt
     4681 tatgtatgga gtgtatagaa ctatttttac ttgtgtcttt tcccttaatc cacgtgtacg
     4741 gaatacagct tataactgcg tatgcagtta tgtttgaagg aggaagattt tctgtggtaa
     4801 ttaagaaata tgtttactca cacccgatca atgtatttat tatcggtaag ctaggtttat
     4861 ctgttgaggt gttttgtgag ttatatggat ttaaacaagg aactctatcg tcttggatta
     4921 ctagagagcg tgaggtagct gatttgccta tttctttttt gtatgctttg tctctagctt
     4981 caggagggaa tatgaatgag gtttatgatt gcttgtgtga gtatgaaaaa gagtataaag
     5041 agtatttgaa aatttcagcc ccaaaacgga ataaaaaata tattgaagat tagagaaaga
     5101 agaaaagaat aatttgtttt ttgactacaa gtagactgat agctactctg ttttttgata
     5161 gtttgatatt ggctgttttt ttgttagaat taatatatat caggtaaata aaaaaagggg
     5221 gctattaatg aatattgatg ggatttataa tgaatcagat tttaaaaaag gcatagtaga
     5281 tattgataga aaagagtatt tatttcctga tgaaataggt ttatttaaaa ttgtaggtga
     5341 aactgtctgt aggtattggg ggaagaaagg aaatataatt ttacttgttg atacgattga
     5401 gggaagaaag ttaaaatttt cgttttggag taatacaaaa tattgttgtg aacctgtacc
     5461 taaaagggaa gtagatgtgg ctaaagaaag tgtacgattt ttacctgatc gatcaaaaat
     5521 aactttaact tgtgaacgaa caagtaatgc taaatttaca agaattagag atatattact
     5581 ttcgactaca agtagtcagt catagagtaa gtacttttgg ttaaaaatca attgaagaaa
     5641 gtggaggaag tatatggcag tagttatatt agcagaaaaa ccagatcaag gagcaaaatt
     5701 tgcttcagca ttggccggaa aaaaattatc gaaaaagagt gggaagtatg aatttaaaag
     5761 tgaaatattt ggagatacta ttgttacttg gggaataggt catttggtag gattatcatt
     5821 acctgaaaag tatgagtggc taccgaataa agataaatgg catttagata atttgccttt
     5881 tctacctaaa ttcaatgaat taaagtatga agtctctaaa ggaaaaaacc aacaattttc
     5941 aatagtgaag tcatgtttag aaaatgcaga tgagataata attgctactg atccggatag
     6001 agaaggagaa aatattgctt ataatatttt caaattatgc aagcagtcga tttttaataa
     6061 accgataaaa agattatgga ttaattcttt ggttcaaaaa gaaattatca aaggatttaa
     6121 aaatctgcga aattctgaag aaacgatctc ttacttcaaa gaagctaatg ctagacaaat
     6181 tgctgactat ctagtaggga tgaattatac agagttgtat tcattaaagt tgcaagaaaa
     6241 aggtatatcg ggtgtggtat cactcggtcg agttcagaca ccggtaaaca cacttgtggt
     6301 tgaaaacgat ttggcaatta gaaattttag accggaaaaa tataaaacga ttgaatgtca
     6361 tacaaccgat aagcaaccaa aagcaatatt taagaatagt cttgaatact ttaaattaga
     6421 aaaatttgaa gaagacacta aaaaattcgg ccttgattcc gccagtactg gaatcataaa
     6481 aaaaattgag gttagtaaaa agcaaacaga accacaaaaa ttgtttagct tgggtggtat
     6541 tcaagaatac gctaataaaa aatggaaata ttctgctaaa aaaacagata aaattatcca
     6601 aagtctatat gatcgtggat atttatctta tccaagaacc gatagtgaat tgataactac
     6661 aaatgaattt gagtatttaa aagcgaattt agacgtgtat aagagtgttt tagggatcag
     6721 tattgataca ccttgtttag agcctaaaaa gcgatttgta gataatgaca aagtattaga
     6781 gcattatgca ctaataccaa caatggatat tcctgatata gcaaatctga cattagatga
     6841 aaaaaatatt tatgagatcg ttactaggcg tgcgtgtcta atgtttgcag aaccttacaa
     6901 gtacgaaaat acaaaagtca cattagatgt aaacggctta gaatttacca cgagtggtaa
     6961 tgtacctata tctttaggtt ggaagcaagt agatactaat ttagatgaat ctgatgataa
     7021 agaggaaaca ttacctaaat ttcatgaaca agagacaata aatatagata ttagatttat
     7081 tgataaatta actaaggaac ctaaaaggtt gactgaaggt aaattattag gtaaaaccgg
     7141 tattatggcg aaattagggc taggaacgcc cgctacacgt tctaatattg tagacactct
     7201 tctaaatagg gaatatatta agatagaaaa cacgaatgtt tatcctactc caaagggata
     7261 tttattatgg gatttgacaa agaataaaga cctattgatt ggaaaaccag aaaatacagc
     7321 gatatgggaa gaatcacttg ggaaaattgc gcaaggtaac tatacacagg aagctttttt
     7381 aagaaatatc tataactata tcaataagac tgtttcagat ttgaaaaaag tagaatttac
     7441 ttcggattat ttagaaaaga ctgtaaatca aaataaattt gatataggta gttatcaagt
     7501 ggaagaaaag aaaaaagttt attctatttt aaatattaat agtggtgaac agttcgtgat
     7561 atttaaaaat atatcaggaa aagcaatcaa taaaaaagtt cttcaagaac tattactaaa
     7621 taatcgtaca gataaagtcc tttccggctt taagtcaaaa gaaggaaaag cttttaatgc
     7681 aagattactt ttcgatccaa atgtgatgaa agtggtaatg gagtttgaaa agaacagtcc
     7741 aaaaggcgat aaaaagagca aacacgatac agtaaatggt tatgaaatag ttgaaaaagc
     7801 aaaagtattt gaaataaaag aattagcaac aggtgaaagt tttattttct ataaaaataa
     7861 tagtgggaaa actttaagtt caaaaacagt caaagaattg ttagaaaatg ggaaaactaa
     7921 agaaaaagta acgggtttta taagtaaaga caataaaaaa tatagtgctt atcttgtctt
     7981 tgataataaa aacaaagtta taaagaaaga attttaattc ttagtggcgg aaaacatttg
     8041 tttttccgct tatttttatt caaaagtacc caaaatatag gtatactgaa ttaaggataa
     8101 atacaaaact agttagagga cagttaaaaa aatacaaaca aattagattg ctattttgtt
     8161 aaattggaag tagaataata ccgttttgat atgtatagtg tgttttacca ataaaattac
     8221 ataaacgtga atatattttc atgaattaaa tgtaaaatga gagcaattag ttttaaaaaa
     8281 gattataggc tgttaatctt agcatatata atccattgga aagggggaga tccaatgaat
     8341 ctaaagaata caaactcaaa caaatctacg ctatatgtaa tttcagatga ttatataaat
     8401 aagctgaaaa aggtggacta tcgtgtccaa aacaactatc gagaacaacg tttatatttt
     8461 aaatcaaact tgaaaatttc taac
//
LOCUS       DAXWOO010000122         8473 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.124, whole genome shotgun sequence.
ACCESSION   DAXWOO010000122 DAXWOO010000000
VERSION     DAXWOO010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.124"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            99..374
                     /gene="rpsP"
                     /locus_tag="ACGE70_002136"
     CDS             99..374
                     /gene="rpsP"
                     /locus_tag="ACGE70_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFF8693757.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            386..637
                     /locus_tag="ACGE70_002137"
     CDS             386..637
                     /locus_tag="ACGE70_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFF8693758.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            761..1552
                     /locus_tag="ACGE70_002138"
     CDS             761..1552
                     /locus_tag="ACGE70_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693759.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTATTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            1722..2243
                     /gene="rimM"
                     /locus_tag="ACGE70_002139"
     CDS             1722..2243
                     /gene="rimM"
                     /locus_tag="ACGE70_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFF8693760.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            2233..2994
                     /gene="trmD"
                     /locus_tag="ACGE70_002140"
     CDS             2233..2994
                     /gene="trmD"
                     /locus_tag="ACGE70_002140"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFF8693761.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            3130..3477
                     /gene="rplS"
                     /locus_tag="ACGE70_002141"
     CDS             3130..3477
                     /gene="rplS"
                     /locus_tag="ACGE70_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFF8693762.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            3787..4029
                     /locus_tag="ACGE70_002142"
     CDS             3787..4029
                     /locus_tag="ACGE70_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFF8693763.1"
                     /translation="MNDGKVKQVPSSTKKKNILLKEVLKRFDHGVTYTETEVNSILLN
                     VFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(4117..4263)
                     /locus_tag="ACGE70_002143"
                     /pseudo
     CDS             complement(4117..4263)
                     /locus_tag="ACGE70_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
     gene            4320..4634
                     /locus_tag="ACGE70_002144"
                     /pseudo
     CDS             4320..4634
                     /locus_tag="ACGE70_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345994.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
     gene            4663..5922
                     /locus_tag="ACGE70_002145"
     CDS             4663..5922
                     /locus_tag="ACGE70_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFF8693764.1"
                     /translation="MKRWKKMMIGSIVLGMSLVFGACGNQEDSSEESANEITVSTWNY
                     ETTPEFKALFEAFEKKTGIKVKAVDIASDDYDTKLTTMLSSGDTTDVLTMKNLLSYSN
                     YALRDQLVDQTERIRELDTDAAEETYEMYDIDGKTYALPYRTDFWVLYYNKKMFDDAG
                     IEYPKNLTWDEYEDLAKKLSKNDGQVYGAYQHIWRSTIQAIAAAQNEANLVEPDYQYM
                     TDYYDRALRMQKEGAQMDFGTAKSTKVTYQSQFEEQKAAMMYMGTWYMAGILANKEAS
                     KTEVEWGITAIPQKKKGESVTTFGSPTAFAVNKNSKKQKAAQEFIEFAASEEGAKVLA
                     GVGVVPSYRTDEIDQLYFNLAGMPTDEISKKAFSPDEIKLEFPIDTYGPAIDKILQEE
                     YDLVLVGDETPEKGTVNMEKRVAAEKE"
     gene            5975..6871
                     /locus_tag="ACGE70_002146"
     CDS             5975..6871
                     /locus_tag="ACGE70_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8693765.1"
                     /translation="MENSINKKATNHLRRKRTWIAYSFIAPNFIGFFVFTLIPILFSL
                     VLAFMKWDSFSTPESAGLDNFRKMANDETFWISLKNTFLYTAAVVPGTLVCSLGLALL
                     LNQKIRGMKLFRTIFFFPYVTSLVAIAVVWNMLFHPTVGPINIFLRRFIEHPPGWTSS
                     SNWALWAIVIVSIWRGMGYYMILYLAGLQSIQKELYEASSLDGANRWKQFLNVTLPSL
                     RPTTFFVTIMLVINCFKVFDLVQVMTDGGPGRATNVLVYQIYNEAFKKFDFGYASAIS
                     LVLFVIVLGVTVVQFSWNKRRT"
     gene            6882..7751
                     /locus_tag="ACGE70_002147"
     CDS             6882..7751
                     /locus_tag="ACGE70_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8693766.1"
                     /translation="MEESVRNERKKAKIRSKNMPFIQWLGMILLIVLALLTIIPFLWM
                     VSASFKANNEVLTIPIQWIPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFT
                     SSFAVYGFSELNFKGRDILFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQ
                     AFNAFGVFLLKQYYSSIPDSLCESARIDGLSEWEIYRKIILPLTKPAIASLTIITFVN
                     TWNDYMGPFIYLSSTENKTIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQK
                     YFVEGIASSGMKE"
     gene            7761..8436
                     /locus_tag="ACGE70_002148"
                     /pseudo
     CDS             7761..8436
                     /locus_tag="ACGE70_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
BASE COUNT         2798 a         1388 c         1754 g         2533 t
ORIGIN      
        1 cgaatttaat caagctgtaa agaaaaaaag ctttacaggg taaaaaaaaa ctgttacact
       61 acatcttgtg aataaaacaa tggaggtgga aataaataat ggcagttaaa atccgtttaa
      121 aacgtatggg ttctaaaaag agtccgtttt accgtatcgt cgtagctgat tcacgttctc
      181 ctcgtgatgg acgtttcatc gaaactgtag gtacttacaa tcctttgaaa gaccctgcag
      241 aagtagtttt aaaagaagac ttagttcttg actggttatc aaaaggtgca caaccttctg
      301 acactgtacg taacatcctt tcaaaagaag gcgttatgca aaaacaccac gaagctaaat
      361 tctcaaagaa ataaggtgac cggatatgaa agatttaagc gacttagtct taacaatcgt
      421 tcgtccgcta gtcacttatc ctgatcaagt caagttagac gtcgtagagt cgaaagactt
      481 ttacgagtac aatctaacag tggctcctga agatattggc cgtatcattg gaaaacaagg
      541 gcgtgttgct aaagcaatcc gcacaatcgt ttacggtgtt cggatcaatg caccgaaaaa
      601 agtgcgtttg aatatcatcg atgataagga aaagtaaata aacgtgctgt actcgaattt
      661 tcgagtgcag cttttttgtt tttttattaa ttgaagaaat gattatttgg caaaacaagc
      721 aagaaagcgt atccttatag gaggagggag tgaacagcca atgagaaaga aaatgttatt
      781 tattccattt gctctattgt taagcgcttg tagttcggtt actcaagaat cagacaatac
      841 acatctatca agcagtatat ctgaaactgt taccagtcca gaaaaaagta gtaccaaaac
      901 ggctaccacc actgaacaaa caatggctac ctcaaataat gagaaaaaga ctgctttaga
      961 ccagttgaaa gaacaacagc caaatgttcc catgcctcta gatgtaccag tctcttcagg
     1021 ctatctcaat atcgcagcga cacatacaaa gcaaggttat tctattttat attacagaac
     1081 agatcgtccc ctcgggttga atgctgacga attgaatcaa gaaacgccta ttgcaaccta
     1141 tctatatcaa tatgggtttg cttctagtca agaaacaatc caagttttgc agccctttga
     1201 gatcgacaca aatggtcagc aagtggactt aggttctcga atcacagggt atcagcaagg
     1261 tgcagcaggt tcaagttttc ttgaatggca agaaggaaac tggcgtattc gaatccgtgg
     1321 gaataatata gaaggccaag atcccttact attagctaaa gaaatcgtag cttatttgga
     1381 agaaaacagt ctaccagcac ctgaacaatt tggtaaaatt actgttgata tgggtgatac
     1441 gacgaatcga gccgtagaag taagctggca agaaccgaaa aatgcctata cgatcactca
     1501 tcaagatcct atgtctgcgt tgaagatggc agtttctatg aagaggttgt gaaatcagcg
     1561 cgctgatctg agcccatcat ttaactccaa gtcacaggaa gtaatcaaaa atagcacgcc
     1621 gcatttttgc ataattttgg cttaatacct taaaagaaaa ctagaattgc ctcttcacac
     1681 atgctaaaat aaaacagaca aaaaagaaat ggggatataa gttgactgaa tacttgaatg
     1741 taggaaaaat cgtaaacaca caaggtatca aaggcgaagt ccgtgttatc tcaacaacgg
     1801 atttcccaga agaacgttac aaaaaaggaa cggtgttaac actattccaa gacggaaaag
     1861 cacctgtgga attaacagtc aaaagtcatc gcaaacataa aaattttgac ttattgagtt
     1921 ttgaaggaca tccttctatc aatgatgtgg aaaaatatcg ggatggcata ttaaaagtgt
     1981 caaaagacaa ctctgtagat ctagctgaaa atgaattcta ttaccatgag atcatcggtc
     2041 tgcatgtatt agaagatgga actgagttag gtaaagtaaa agaaatcttg tcaccaggtg
     2101 ccaatgatgt ttgggtcgtt caacgtccga aaaaagctga tatattgctt ccatatatcg
     2161 cttctgtcgt caaagaagtc gatttagaag ctggagtggt ccgtgtagaa attccagaag
     2221 gactgattga cgatgaagat tgatgtattg actttatttc caagaatgtt tgaaggtcct
     2281 atgggggaat ccatcatcgg taaggctgta gataaaggat tattagatat caatatctct
     2341 aacttccgcg attattcaga taacaaacat cagacagtag atgattatcc atacggcggt
     2401 ggagcaggta tgctgctcaa agtccagccc atctatgata acatccaagc cattgaaaaa
     2461 gctgcaccgg atgtcaaaaa aagagtaatt cttcttgacc cagctggtaa acgattcgat
     2521 cagaaaatgg cagaagaatt ttcacaagaa gaacacttag tattcatttg tggtcattat
     2581 gagggatatg atgaacgtat ccgcacatta gtgacagatg aagtttcctt aggagattat
     2641 gtccttactg ggggagaatt aggtgcgatg gtcatgatcg atgcaacagt tcggcttttg
     2701 ccagatgtgt tagggaatca gacttcagct caaacggatt cttattcaac cggtttattg
     2761 gaacatcccc aatatactcg cccggcagaa tataaaggga tgaaagtacc agaagtattg
     2821 acaaatggaa atcataaatt gattgaagaa tggcaattaa aagaatcact aagaaggacc
     2881 tatcaacgtc ggcctgatct tttggaaaca cttgaatgga cacctcaaat gacgaaattt
     2941 ttagaagaaa tcaagaaaga agaacaagaa aaaacagcag atgaagccaa gtgacttttt
     3001 ctaaaatatt ctttacagta ggttgataat atgttagtat tttatggtga gtgcaaaagc
     3061 acttaactat tacgatattc cgctatgaga aaagctcata agaatatttg gaataaggag
     3121 aagaaaacca tgaatccatt aatcgaagaa ttaacaaaag aacaattacg ttctgacatc
     3181 ccagcgttcc gccctggtga cactgtgcgt gttcacgcaa aagttgttga aggtactcgt
     3241 gaacgtatcc agttatttga aggtgttgtt atcaaacgcc gcggtgctgg aatcagcgaa
     3301 acttatacag tacgtaaagt ttctaacgga gtgggcgttg aacgtacatt cccattgcat
     3361 acaccacgtg ttgcaaaaat cgaagtagtt cgctacggta aagtacgtcg tgcaaaattg
     3421 tactacctac gtgcattaca cggaaaagct gctcgtatca aagaaatccg tcgttaatca
     3481 ttgttatcag actgagaaag aacccttggt attcaagggt tcttttttct tagcatgtaa
     3541 tttgaacacc ctgatccagt ttttttcttt aattattggt aggggagaat aatggattag
     3601 aataaatcta cctataagta gtagtaaaaa aaagagcact atgataatat ctttcttaag
     3661 agtaaccgct ttaataaaaa atccagaata ttctaacata aatggagtat tcttagaatc
     3721 aaaaggagaa agaagatggt ttatacttat acggagcaag aaaaaatgaa agtttgagac
     3781 acattcatga atgatggaaa agtaaaacaa gtaccaagtt caacaaaaaa gaaaaacatc
     3841 ctattgaaag aagtcttgaa acgctttgac catggagtga cttatacaga aacagaggtt
     3901 aattcgatac tgctgaacgt attttctagt ggcgattatg tcgaacaaag acgttatcta
     3961 atcacatttg gctttttcaa aagaagttca gacggtcggg catatcagat gatgggaatt
     4021 gaaaattaga agttttaatt tatactcatt gaaaaaagtt caagtatgcg atattggttc
     4081 tacaccgcat acttgaactt tttttgtcat ctgtatctat aatacaaagc caagaacgtt
     4141 attcaaaata tggatagcta tgataaaagc taatgagcga ttcgcagtta aataagcaat
     4201 cgtcagtaaa atcgttccag gagcgtacat gaaaaaatca attggatgcg ttacatgaat
     4261 aaagataatt ctcttttatt tgtacgaata aataccaaaa cgtacctcct aaaattagat
     4321 tcaaagaaga tagcaaagag gaaaaagata gttctgcagc agctatagcg gcatgtggat
     4381 tgctagaact ttccaaacaa ctgcctgttt cagatcctcg tcatttcgat tacgaagaga
     4441 tagcaatcaa aatcttggaa gaattacaga aaaactatac aacgaaacga gttaacggat
     4501 caaacggtct gcttctccat gccgtttatg ataaaaacag cttaaaaggt gtagatgaat
     4561 gtgtgatctg gggcgattac ttttatgtag aaggaatcac tcgtctagct aaaacatggt
     4621 actgttactg gtgatgtata agagaagagg aggattcata taatgaagcg atggaaaaaa
     4681 atgatgattg gatcaatagt tttaggaatg tcactcgtat ttggtgcttg tggtaatcaa
     4741 gaagacagta gtgaagaatc agcaaatgaa attaccgttt cgacatggaa ttatgaaaca
     4801 acacctgaat tcaaagcgct ctttgaagca ttcgaaaaga aaacgggaat caaagtgaaa
     4861 gcagtagata ttgcttcaga tgattatgat acgaaactca ccacgatgct ttcttctgga
     4921 gacacgacag atgtattgac gatgaaaaat cttttgtcgt attcaaatta tgctttacga
     4981 gatcagttag tcgatcaaac agaacgaatt agagaattag acactgatgc tgctgaagaa
     5041 acatatgaaa tgtatgatat cgatggaaaa acatatgcat tgccatatcg aacggacttt
     5101 tgggtactat attacaataa gaaaatgttt gatgatgcag gaatcgaata tccaaaaaat
     5161 ctaacttggg acgaatatga agatttagca aaaaaactta gtaaaaatga cggacaagtt
     5221 tacggagcat atcaacatat ctggcgttcg acaatccaag ctattgcagc tgctcaaaac
     5281 gaggcgaatc ttgttgaacc agattatcaa tatatgactg attattatga tcgagcattg
     5341 aggatgcaaa aagaaggcgc gcagatggat tttggcacgg ctaaatcaac gaaagtaacc
     5401 tatcagtctc aatttgaaga acaaaaagca gcaatgatgt acatgggaac ttggtatatg
     5461 gctggcatac tcgcaaacaa agaagcaagt aagacagaag tagaatgggg aattacagct
     5521 attcctcaga agaaaaaagg agaatcggtc acgacgtttg gctcaccaac agcatttgct
     5581 gtcaataaaa actcaaagaa acaaaaagcg gcgcaagaat ttattgaatt tgcagccagt
     5641 gaagaaggag caaaagtatt agcaggagta ggagtagttc cttcatatcg tacggatgag
     5701 attgatcagc tttattttaa tctcgctgga atgccaacgg atgaaatttc taaaaaagct
     5761 ttttctcctg atgaaatcaa acttgaattt ccgattgata cgtatgggcc tgcaatcgat
     5821 aagattttac aagaagaata tgacttagtt ttggttggag atgaaacgcc ggaaaaagga
     5881 acggtaaata tggagaaacg tgtagctgcg gaaaaagaat aatcatgaaa ggagagaagc
     5941 caagagataa aacactcttg gtttcgtcat cgaaatggag aatagtataa ataagaaagc
     6001 tacaaatcat ttgcgtagaa aacgaacgtg gatcgcttat tcgtttatcg ccccgaattt
     6061 cattggtttt ttcgttttta cattaatacc aatcctgttt tcgcttgtac tagcatttat
     6121 gaaatgggat tctttttcaa cgccagaatc tgctggacta gataactttc gtaaaatggc
     6181 caacgatgag acattttgga tttctttgaa aaatactttt ttatataccg ctgctgtagt
     6241 gcctggcaca cttgtttgtt cgttagggct agctttacta ctaaatcaga aaattagagg
     6301 gatgaagctt tttcgtacga tatttttctt tccgtatgtt acttccttag tagcaattgc
     6361 tgttgtttgg aatatgttgt ttcatccaac agtgggacct attaatatct ttttaagacg
     6421 atttattgaa catcctccag gttggacttc aagttcaaat tgggcgcttt gggcaattgt
     6481 aattgtcagt atttggcgag ggatgggtta ttacatgatt ctataccttg caggactaca
     6541 aagtatccaa aaagaactat atgaagcgtc ttcgctagat ggagcaaacc gttggaaaca
     6601 atttttgaat gtgacattac cttcgttgag accaacaacc ttctttgtaa cgatcatgct
     6661 tgtcatcaat tgtttcaagg tgtttgacct tgtgcaagtg atgacggatg gcggccctgg
     6721 tcgagcgaca aatgtgctgg tttaccaaat ctacaatgaa gcattcaaaa aatttgattt
     6781 tggttatgct tcagctattt cacttgtttt gttcgtaatt gttttgggcg ttacggttgt
     6841 tcaattttct tggaataagc gtcgtactta ggaggtgtga tatggaagag agtgttcgaa
     6901 atgaaagaaa aaaagcaaaa attcgttcaa aaaacatgcc ctttattcaa tggttgggta
     6961 tgattctgct gattgtatta gctttattga cgatcattcc ttttttatgg atggtttctg
     7021 cttcattcaa agcaaataat gaagtgttaa cgattccgat tcaatggatc cctaaaactt
     7081 ggcatttaga gaattatctg gttatttggt ctcgtattcc tttagtgaca ttttttaaga
     7141 atacagcttt tttaagcgtt gtgatcacgt tgattcagtt gtttacttct agttttgctg
     7201 tttacgggtt ttctgaacta aattttaaag ggcgcgatat tttattttta gcatatatcg
     7261 gcacgattgc tgtcccttgg cagtcttata tgatcccgca gttcattatg atgagaaaag
     7321 tgggtttaac agatacgttg tggtcgttgg tgctattaca agcgttcaat gcttttggtg
     7381 tgttcttgtt gaagcaatat tacagtagta tcccagattc tttatgtgag agtgcgcgga
     7441 tcgatggctt atcagagtgg gaaatctatc gcaagatcat tttacctctt acaaaaccgg
     7501 cgattgccag tttgacgatc atcacattcg ttaatacatg gaacgattat atggggccat
     7561 tcatttattt gtcttctacg gaaaataaaa caatccagtt agggttgaaa atgtttattg
     7621 ggctatttga tgcagagtac gcattaatca tggctgcctc agttgtttct gttcttcctg
     7681 ttgcttttat atttttagcg atgcaaaaat attttgtaga agggatcgct tcatcaggaa
     7741 tgaaggaata ggaggtgatt atgaaagagc agcgttttga caaaaatgtt tatatggaat
     7801 tcttttattg gttgtatcaa tcattggcaa gtagcctttg tttttggtgt gtcaatatct
     7861 tgtatatagc agctgcgttc cttttgtcga tagatggaag gaatgtcttt tcctttattt
     7921 tttctttact ttttattggt ccaggttgca tcgtgatgat aggtatgaca gaagaattag
     7981 caaaagaaaa aagtttttca ccagtacgtt tgttcataaa gacgtttcgc caattttggc
     8041 taaagggatt tttttattgg ctatttgctt ggatagttag tgtgataatg atttttgatt
     8101 gtttcttttt tatacgtttt tcttatggaa aatggttgat tccgttgttt gttttacttg
     8161 cttgttttac ttgcttgttt atcagtttcc tttagcatca actgctggta ttttcaggtg
     8221 agaaatccag cgtcgaaacc aaatcaagtt ctacggatag cgttctacta tacgttgaaa
     8281 aaatggtatg tgagtttact agatttttta ctattgacaa gtttgtttct cttctttttc
     8341 gtaaagcccc aatggtgtat tctactaggt ccttctatcg tatttggttt gatttacttc
     8401 aacaatcgta aattgatgag aactatggat ctgtagactt tataaattga aactttcaat
     8461 taagaatgac agt
//
LOCUS       DAXWOO010000123         8409 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.125, whole genome shotgun sequence.
ACCESSION   DAXWOO010000123 DAXWOO010000000
VERSION     DAXWOO010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.125"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            254..733
                     /locus_tag="ACGE70_002149"
     CDS             254..733
                     /locus_tag="ACGE70_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFF8693767.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            766..2994
                     /locus_tag="ACGE70_002150"
     CDS             766..2994
                     /locus_tag="ACGE70_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFF8693768.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            3123..4766
                     /locus_tag="ACGE70_002151"
     CDS             3123..4766
                     /locus_tag="ACGE70_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFF8693769.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(4839..5384)
                     /locus_tag="ACGE70_002152"
     CDS             complement(4839..5384)
                     /locus_tag="ACGE70_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFF8693770.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5407..5892)
                     /locus_tag="ACGE70_002153"
     CDS             complement(5407..5892)
                     /locus_tag="ACGE70_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFF8693771.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(5882..6694)
                     /locus_tag="ACGE70_002154"
     CDS             complement(5882..6694)
                     /locus_tag="ACGE70_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFF8693772.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(6833..8365)
                     /locus_tag="ACGE70_002155"
     CDS             complement(6833..8365)
                     /locus_tag="ACGE70_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFF8693773.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
BASE COUNT         2843 a         1599 c         1654 g         2313 t
ORIGIN      
        1 acaaaaaaga tgttggtgaa gttcaccaac atcaaaaagt atagagccaa aatattccaa
       61 catcatcttg gactttctac atgttactaa tgagcgtgta aaagaacgag atggtgtttt
      121 tatttctatc aatgaagctc tttttctttg caatcctcac ttcaatcctc tataatgtcg
      181 catatagttt agtaaatgag taaactacta atttacttat ttactaaaaa taaaaggatg
      241 aggagttaaa ataatggaaa taccaacaaa tttaaaacat aaaccagtaa tcgttgtaga
      301 agactatgat aaagttgatg gaagaaatgc attaaatatg gatgcaaaag ggctttcact
      361 gggccttgct cagtggaatg atcgtggaaa agtcgatatc tcaggcaagg tatggcgaca
      421 tactggagaa aaatggtcac gacagtcgga agaattacca atcacacgta ttttagattt
      481 agcgatctta gctgcgcaag gaagtttata cttccaagat gcttatcgtt atgaaaaatt
      541 ttatgatcca gaaaatccag tcgtagacat cattggatta caagggaacc gaatgacggt
      601 agaagtagat accaaaaatc caaagatcga tgaagacatc attttgtatt acgatacact
      661 acaaaaagac ggagaactga ttggggaacg attccgtatt ttaaaacgtc ttttagatga
      721 gctaggatat taatagacag agatgatcaa aaggggaaga gaagcatgga gaatttctca
      781 ctaacagaaa accgaacctt ttcacggcta gaaaagcaaa tgatctggaa gaaaccgaaa
      841 agccatcgga caagtgcaga agaatggcgg atcgcctccg aaatcaaagg gaattggcaa
      901 gatcctgaaa tgaaaatcag taacgtactt ttggaaggag atgccgggtc ggggaagaca
      961 caattagcca aagcactatc tgctgatctg caacttccct atacaaaagt gacctgtttt
     1021 gcagatatgg acaaatcaga tgttttcgga gccttattac ctgtagtaga ttctaataag
     1081 gaacaagacc aagaattact agaagctatt taccaaacag atacacttga agctgtactg
     1141 acactgatcc agaaccatta cggcattgat cctttttctg ctaaagaaaa attagcagag
     1201 ctagttcaaa ggatcgaagc tgattatcca gatgcaatcc attacagcta ttacccttct
     1261 gaaatcgttc gagcgattga aaaaggctat ttattagaga ttcaagagcc gactgtcatc
     1321 cgagatgcct ctgttcttgt ggcacttaac tcagcgctcg aaccaaatgg gatgttgaat
     1381 ttaccgacag gaatcattcg tcgccatcca gattgcgtca ttgtgatcac tacaaatcga
     1441 aattaccaag gaaatcgccc attaaatgaa tctttaagag atcggatgca acatgcggaa
     1501 aaaatggatc ttcctgcctt agaagtcatg gcagaacgtg cgatggcgaa aactatgatc
     1561 caaaaacaag aacttctctt aaagatggca gagattatcc gtttgttgga tgaaactgca
     1621 aaagctaacg ccatcaaagg tgtagcagga atgcgttctt atttttattg ggcaaatacc
     1681 tataagcaag gacaagattt actccagtcg atttatccaa aagttttgta taaattatcc
     1741 actgattcag atgaattggc aattcttgaa caagctttgt ctgacagtgg attgctactg
     1801 gaacttcagg acatattgag aaaagaaaaa tggggtcatc ctcaatctga gcatacaaaa
     1861 gaaagaacca ttagcgccga agaagcaaat gaacgaaaca tcactgaaaa tgctgaagaa
     1921 attctgcgct tagatgaaaa gaaatcgttg tctgctgaag aaccaaatga gggagaagag
     1981 aaacaagaag aaacaataga aaaaaacgag attcctcctc aaatgcaaaa agaacgcagt
     2041 agcgatatcg aaggaaaaaa tcaaaaagaa caacaaacag gagatgattc tcaagaaatg
     2101 tctgcttctg acatggaagc acatgataag gaaatcaaga agcaactgaa taaagaagcg
     2161 cgacaaatca tgaaggaaac gattcatgaa aaagaaggtc tcatcgttca ccgaccagct
     2221 ttccaaggaa aaacgccgga agcacgtgag ctacacatgc aagtaatgcc aatagtagaa
     2281 tcacttagcc ggcaaatttt agagttactg gataatgaac aaacagagac gtaccaaaaa
     2341 ggaaaatacg aaggtcagcg gttcaatgca agcaaagtag catatggtga tttgagaaat
     2401 tttgacaaaa agaacccgcc acatgagcaa ccttctttag cggtagctgt acggatcgat
     2461 gaatctgggt caatgatacg ggatgatcgg atcgaagcgg caaaaaaagc agcgattgcc
     2521 attgccgaat tttctaagaa agtcgatatc ccattgttga tttacggaga tacagcagac
     2581 cgttcgtcaa gagaaaagac ttctttgttt tcttataaag aatttgaaga cggatttcaa
     2641 tggttaaatg aaaaattagt cactatgaaa ccaaggcaaa acaatcgtga tggtgctgca
     2701 cttcgtttag cagcagaaaa attaaacaga caatccgcaa caaccaaact gttgcttaat
     2761 attagtgacg gtcagccaaa agcgttgccg gattatacag gcaaaaaagc aaaagaagat
     2821 atccagtctg ttttgaaaga atatgaacgt caaggaatcc tatttgtctc cgcagctatt
     2881 ggccaagaca aagaagaaat caagtcgatc tacggtgaaa gtcgttttgt agatattact
     2941 gacttgaatg aatttcccaa gcaaatgatt caactgattg cccgctatct atgataaaat
     3001 aaagctcaac gaacgcactt agcagattcg ttgagctttt cgtttctagt taccggtaac
     3061 aaggatactt ctaaaataga atcggttata atcaagaggg accgttaaag gagtgaaacg
     3121 atatgataca agaacaaaaa tggtggaaag aagcagtagg ctatcaaatc tatcctgcta
     3181 gtttcaaaga tagcaatggc gatggtcaag gagacatcaa tgggatccgt gaaaaactta
     3241 attatttgaa agatcttggt atcggcttta tctggatcaa tccgatctat caatctccat
     3301 ttgtcgataa tggttatgac gtaagtgact atcaagatat agaagagaga ttcggaacga
     3361 tggaagattt tgatttattg ctaaaagaag cacatgaatt agggatcaaa gtgattatgg
     3421 atcttgtcat caaccatagt tcggatcagc atcaatggtt tgaagaatcg aaaaaatcta
     3481 aagaaagccc gtatcgcgat tattacattt gggtcgacgg ggtagatggc aaagaaccga
     3541 ataattggac atcgattttc ggcggttctg cttgggagta ttctcatgaa actggacagt
     3601 attatctgca tgtttttgca aaagaacaac cagacttaaa ttgggaaagc gagaaattga
     3661 aagaagaact attcaatatg atccgatggt ggttggataa agggatagat ggatttcgtt
     3721 tagatgcgat ttctcatgta aaaaaagatg aatattcagt aaaagccaca gagaaccctt
     3781 tctcaccctt tcaaaatgtc tcaggaatcg aagaacattt gaccgatttg aaacatgttt
     3841 ttgaagagta cgatatcatg actgtcggag aagcaagtgg tgtcactgca gaagaaggac
     3901 cacaatgggt agggaaagac ggttatttcg atatgatatt cgaatttgat catatccata
     3961 tctggcagca agaaaaagaa gggcaattag atgtccttaa attgaaacat gcattaagcg
     4021 cttggcaaac ttcattggat ggtatcggat ggaacgcttt atatatggaa aaccatgatg
     4081 ttcctcgtgc cgtttcagtg tttggagata ctcgtcccga tttttgggct atgtctgcaa
     4141 aagcaatcgc tatgatgtat ttctttttgc aaggtacgcc atttatttat caaggacagg
     4201 aaatcggaat gacgaatatg ccctttgaat cgatcgatca agttgatgca gtagattcga
     4261 aaagacttta taaaaggctg cttgcggaag gtaagacaag agaagaagca ttggatatca
     4321 ttcgtgaaac aacgagagac aacagcagga cacccatgca atggacttcg gaacaatatg
     4381 ctggtttttc tacacatgaa ccttggctga tcactaatcc aaataccaaa acgatcaatg
     4441 tagaacaaca agaatatgaa ccagagtcgg tcctgcaatt ctataagaac atgatccgca
     4501 ttagacaaac caataaaggt ctgatatacg gttcttataa agaatatctt catgaacatc
     4561 cacaacttta tgtctatgaa agatatttag aagatgaaca atatcttatt atggtaaact
     4621 taacagaatc acttgctgac tatgaacttc caaaagaagc agatcagtcc tggacattac
     4681 tcttgtcaaa ttcctctagc ggagaatttg aagctaaagg catactagca ccttatgaag
     4741 ctcggttata taaaacaaac aagtaaaact tcctcgttaa agttcattag ttggactttg
     4801 cgaggaagtt tttcagattg ctctgtcaat tactcatttc aataattcgc tctaggacca
     4861 ccgatatatt taggacgaga gcgaagggct gtcacgtgag cttgtccaag ggttttcaaa
     4921 cgaggtctga caggtacttg gacatgcttg atatgcatac cgattgatgt gtcaccgata
     4981 tcgataccgg cttgtccaac tacgtgttcc acttcgacag gatcattgaa ctggtcaaaa
     5041 gcagcaacag aacaagcacc tccagcatgt agtgcaggaa caacagagac aatttcgaaa
     5101 ttttttgctt ctgccactgt tcgttctact actaatgctc ggttcaaatg ttcacaacct
     5161 tggacagcta gcgcaatttc tttaggatcc aacaactctt ttaacgtacg gatgatccat
     5221 tgaccaactt ccgcactgga attttttcca atatgtcctc caacgacttc gcttgtgctg
     5281 catcctaaaa caaaaagatc accttttttc aagtgtgcct cagccaatat gtcattaact
     5341 atttcagtca attggtcttt taattctttt tctgacaact tcattttcta aaaccttctt
     5401 tttcaattaa tattgcttcc gtgcttgaat acgttgaaga cttgcagtta taggaaaggc
     5461 aagtcccatt ccaatcagat tttggaagat gtttcctggt atagaagcca ctccagccgg
     5521 ccaatcatat aaaatccaac cggcgatacc atagccgatg atcataacta agcttcctaa
     5581 taaaagccct aatccccaca tacgagtaga agatttgctg aaagaaccag caacatatcc
     5641 ttgtaacccg tggatcacta atgaaaaaac tgcccactgc ggatatcctg aaatcaaatc
     5701 gatcaataga ccactggaag caccaacaac taatccaccc gttggaccat aaagaaaagc
     5761 agcggtatag atcccggcct cgcataatgt cacaaatcca ttcgtagccg gtacagggat
     5821 caaaattgtt aatgaaaggg caactgtcaa cgcaatcagc atagcataga tcgttaattg
     5881 cttagttttc atttctcatt cctccttttt gaataccgcc agaccagaca cttccggtat
     5941 gatcgttcat cagcacgcca ttagaaatac aatgggcaac aaatttttta ctttctgcta
     6001 ctgctttggt aaaatctgcc cctaacacaa gattcgctgt tattgcagaa gaaaaggcac
     6061 aaccggcccc gtttactgtt gtagtgtcga tttttttatg agaaaatacc tcataatgat
     6121 caattgaata gagaagatcc acagcttcat ctccagccat ccttgtgccg cctttgatca
     6181 caatgttttt tgttcctgaa gcagtgagtt ctttagcgag atggatcatt tctgttaacg
     6241 aagagggaag agcttgacca gaaagtaaag cagcttctgc caaattaggc gtaatcatcg
     6301 taacaagtgg aaaaagttct tcgatgagct gcgaaatata caattgattg atcacagctt
     6361 cagtttcttt gaatgccaaa acagggtcta aaatgattgg gatattttgt tttgttttta
     6421 tgaagtctcg aacaaccatg attgcttcac ttgaattcaa taacccaatt ttgataccat
     6481 ctaatgaagt cattttatca atcgaaacta actgtcgttt aagcaattcc aaagatatag
     6541 attccagcat aaatcgctca ttaatatcta gtgcccctat agaagtcaag gcgcttagtc
     6601 cgaatagacc aaaattctca aaagttttta aatctgtttg gattcctcca ccagcaaatg
     6661 gatcagaacc gccaattgtc aagattcttt tcataacatt ttcctccatg aataagagta
     6721 taacgaaatt ttcaaaaaag aaaaacagcc aattggcata tttttatctc atccaattta
     6781 aaaaaacaaa aagaaaagaa agaccaaaat ggatctttct ttccttaaat agttaggact
     6841 tttgtgattt taattctttc tcgatttgaa gccgttgcac tttcattgta gctgttcgcg
     6901 ggattgcatc aaaatcacgg atgataggga cgttcaagcg aggaagttca tggacttgct
     6961 cccaccatcg attccaatcc atttctttat caggagctaa agcaataatc ggttgtggag
     7021 aatgattttt atcccttaca atcactactt ctgccaaaaa atctaacgaa tctaataaaa
     7081 agtcttcaat agctaatgta ctattgatcg tttcgatcaa atccacttgc cgatctttca
     7141 aaaataggtg gccatgctta tccataaaac cgtaatctcc gctatcccac caatcgccat
     7201 aaacattttc ctggaaccgt gcatcttcct tgtaataagt caaagcccgt cctttagaca
     7261 gtaattgtat atgaccatct gtgttttcag gtaggacatt cccaactgaa tctgtaatcc
     7321 gagcgcttgt catgtcttcc agtccaacgc ccatatctct tgcatcactt gttttcaaac
     7381 tctctagtgt atgagcctta agaatcattg gtccgcattc actttggcca tatacttgaa
     7441 gaaataccgc atcctgtgcc atcgacgttc gcaaaaatgt cttcattgtc tggttgttta
     7501 tagcatcaaa agtggagtga tagtatcgga tacccgaaaa agcttctgga tgctccttag
     7561 cagtaaaacg ccactgaaca aagttattcg gatgggtctc tagtgcaatt ggccggtgag
     7621 cttccaacat atgaacaact tttgaacttt gtgcgtttgc taaaggcatc ataggaaatc
     7681 ccatggacat caaagaagag ataccaatat tgaatctgga atgcactgga gagatgtgaa
     7741 aagcgattaa tttcttttcc gaaatttttg tgaaaactgt tttttgccat ttcgtccgcc
     7801 atcccataga atgagcagaa tgacaaataa gtttagggat accagttgta ccagaagtat
     7861 gggtcatata agaaatttca tttttgtcta aaaatgattg agaaacagac aaagcaggtt
     7921 gttccattaa ataaagaata ggaatctgtt ttgattttgt tccatttgaa atttctctca
     7981 ctcgcttttt tgtctcatca tcaaataaaa tgaaagggtc ttctaaacga tcgataaata
     8041 cttccattgt tgttgttggt agatgataag aagtcatgac aggcactgca ccaaggtaag
     8101 acacagaaac agctagaagg tatgtgtcaa aagcggaact cttataaata ataactttgt
     8161 ctccggattt cactccaaga tgagccaatt gataagcccg tttcaacaca ctttcgcagc
     8221 attttttgta agttgtttca agtcctaatt ctgggaaagc tgaaagtggc tcatcaaaga
     8281 taatcggggt ctcggaaaag ttagctgcag cttcttgaaa attcgtaaat aagttcaaag
     8341 gggaatacgt tatggtctcc atcattcaca ctccattatc ttttgttaag taatatacaa
     8401 attcaagat
//
LOCUS       DAXWOO010000124         8356 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.126, whole genome shotgun sequence.
ACCESSION   DAXWOO010000124 DAXWOO010000000
VERSION     DAXWOO010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.126"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            12..839
                     /locus_tag="ACGE70_002156"
     CDS             12..839
                     /locus_tag="ACGE70_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8693774.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            complement(1112..1882)
                     /locus_tag="ACGE70_002157"
     CDS             complement(1112..1882)
                     /locus_tag="ACGE70_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFF8693775.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            2100..3167
                     /gene="ulaG"
                     /locus_tag="ACGE70_002158"
     CDS             2100..3167
                     /gene="ulaG"
                     /locus_tag="ACGE70_002158"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFF8693776.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            3185..3646
                     /locus_tag="ACGE70_002159"
     CDS             3185..3646
                     /locus_tag="ACGE70_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFF8693777.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            3666..5150
                     /locus_tag="ACGE70_002160"
     CDS             3666..5150
                     /locus_tag="ACGE70_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFF8693778.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            5193..5492
                     /locus_tag="ACGE70_002161"
     CDS             5193..5492
                     /locus_tag="ACGE70_002161"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFF8693779.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            5507..6145
                     /locus_tag="ACGE70_002162"
     CDS             5507..6145
                     /locus_tag="ACGE70_002162"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFF8693780.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            6150..7010
                     /locus_tag="ACGE70_002163"
     CDS             6150..7010
                     /locus_tag="ACGE70_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFF8693781.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            7000..7710
                     /locus_tag="ACGE70_002164"
     CDS             7000..7710
                     /locus_tag="ACGE70_002164"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFF8693782.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            7942..>8356
                     /locus_tag="ACGE70_002165"
     CDS             7942..>8356
                     /locus_tag="ACGE70_002165"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8693783.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKV"
BASE COUNT         2747 a         1315 c         1799 g         2495 t
ORIGIN      
        1 aggaaattgt tttgattaaa gtagatggag cattactaaa agaataccga aagaaaaagg
       61 ggttaacaca agtacaatta gcagaaggca tttgtgaaca agcaactgtc agcaatatcg
      121 aaaacaagaa tatgtcgcca ggcatcaata tcttaagtgc gctttgcaaa aagctgaatg
      181 tcgagatctc cgatatatta gtagaaacag aagaagacaa aatcaggaaa aaaataaatg
      241 agattcaatc attgaattct gtatcaaaat accaagaatc ttataaaatt ttacaaacga
      301 tcgatcagga aaaagtaagt aattctgatc ttttaaatca aattttattt tatcaaggac
      361 tgcttgatca tctttatgcg aaaaaaccag atgcagcatt attttatttt aatcaagtgt
      421 tggaacaaac gaccgaaaca gatatccatc acttacaagc agctgcaaac gtggcaatga
      481 tctacttgtc aaaaggagaa ttagattttg cgaaagttta tgtggagcgt acacttaaaa
      541 tattgagtga gactgatttt gataatctga tggtctgcat tgtatattat gatatcgcca
      601 cctattaccg taaaatcgag gattatgata aagcaatcca attatgtgag aagggtattg
      661 agtataataa gaaacataaa tcgacttatg cattggaata cttgctttat gaaattgctt
      721 cttgccataa gcaattagga gaagatgatt atttagaaag atatatggac gcaaaaaaaa
      781 tagcgcgttt caacggaaat gattacgcgg taaaggtgat tgaaaacgac ctgaaataaa
      841 gtggttgtga aatcagtgtt ctgatttgaa taccgtttat tcgaaaaaag gaggtgtgac
      901 ttttgtcata cctccttttt tcgatattgt ttgattccta tttaggcaac atataacggt
      961 tataaaagaa gcaatagatt atgatggtat cgattggatg gatacatctt gggattcctt
     1021 tatcaatttg agcgtagcgt gtttttcatt tatcgataaa tttgttattc atttttttga
     1081 taatcctatt tacatattta cgtcctggtt cttaaaatga atctacttga atggttttta
     1141 tccccgtatt tctgatctcc tctaatactt tttcagaaca aaatttatca gtgatcaaat
     1201 aatcaaagac tgaaatatca gcaatcttga actttgatgt cagtccgatt tttcgatgat
     1261 cagcgactaa aatcttacat ttgattgtct tgttgaccat catttcgttg atcgttgatt
     1321 catttaatat ctttgttgtg accccatgta ctacatcgac tccgctacag ccaatgatac
     1381 aaatgtctgc attgactgaa gaaatcgtat ttaatgcgat gtcgcccact agtgcttctt
     1441 ttggaaagcg catctctcca ccagttaaaa tataactaca attatctttg tgcggagagt
     1501 aattgatttt cacgttgttt gtaatgactg tcaaatggtt agattgaaga aaatccagtg
     1561 ttttcaacgc agtcgagcta gaattgataa acagcgtatc ataatcgtta atgtatttac
     1621 tagcttctct ggcaatcgct tgtttaatcc tttcaggacc agattgatca aaatcagtct
     1681 ggtttttgta attataagaa caataaccgt aattgcgtat gatctcgttt ttttcttcta
     1741 ataaatttaa atctcttcgt atcgtactaa tagatacatc aagggattca gctaattcga
     1801 aagttgatag ttgttcagtc tgctgaagca gttccaaaat ttctttttga cgtttttcaa
     1861 tattgacaag tgagtttttc atgacttttg cctcctgagg gacatcttga tccatggatt
     1921 tatttgacta gttgttcatt taaacattca tatctgtctt tattatactg aaacaatcat
     1981 tgattactga aagaaaatca tataaaaaac aaacggtcat ataaaagggt tttcatggat
     2041 gctgatacac attaatattt acttgtaagt tggaacaacg atatgggggg agcaagcaaa
     2101 tgagtaaaac tgtcaatgaa gtaacaaaag aaaagtggtt agtagaaaca tttcctgagt
     2161 ggggcacata cttgaatgaa gaaatcgaaa cagaagtagt ggatcctgga acttttgcta
     2221 tgtggtggct gggctgtacg ggaatttggc tgaaaagtca tgaaggaacg aatattttat
     2281 gtgatctatg gtgtgggacg ggaaaacgta gtcatgggaa tggcatgatg aaaaaaggcc
     2341 atcagatgat gcgtatgagt ggcgttcaaa atatgcagcc taatttacgt actcagcctt
     2401 tcgtaatcga tccttttgcg ataaaaaatg tagacgcttt aatcgttaca catatccatt
     2461 ccgatcatct agatatccat acagccgcag cagttcatca aaattgtcct gaagcaacat
     2521 tcattgggcc aaaagaagtg gttcgcacgt ggcaaaaatg gggaattcct gaagaaaaaa
     2581 cgattacagt aaagccaggt gatgcagtca aaatcaaaga catcaatgta gtagctttag
     2641 aagcgtttga tcggacagcg ttggtcactt gtgaagatcc agaagaggta ttaaaaggaa
     2701 aaatgcctca ggagatggat caaattgcag taaactattt attcgaaaca tcaggaggga
     2761 atctttatca tgcaggagat tcacattact ccaatttatt tgccaaacat ggtaatgaac
     2821 acaaaatcga tgtctgttta ggggcctatg gtgaaaaccc acgagggata acagacaaag
     2881 ttacttctgt tgatatgctg cgcatggcgg agtcattgaa tgcaaaagta gtgattcctg
     2941 tccactacga tatttggtct aattttatgg ctgatccaaa agagatcact gaaatttgga
     3001 aattcaaaaa ggatcgcctg aagtatggat ttaaacctta tatctggcaa gtaggcggga
     3061 aattcatcta tccgacaaac aaagatgatc ttgagtttaa tttttatcga ggatttgaag
     3121 atgtcttctc gattgaaaat gatacaccgt ttccgtcgtt tttataagaa agaaggggag
     3181 tttcttgctg aaatactttt ttgaccatca attgattcat tacccagata aagaaatcac
     3241 ggattggcgt gaagctatcg ccgaaagctg tcttgtattg atcgataagg aaattattga
     3301 tgaaacgtat gtggaggaga tcatccattg tgtagaaaaa tacggtcctt atattgtgat
     3361 cgcacctcaa gtagcaatgc cgcattcttc agaaaaaagt gatggtgttt ttggaacagc
     3421 tatttcattt accaagttga aacagccaat ccgttttgaa agtgaagggg agaatgagga
     3481 gaaaacggct agtttgtttt ttacactcgc agcaaaagat ccagaagaac atctagaaaa
     3541 tatccaaaaa ttatccgaat tattaatgac agatggattg gttgagcgtc tcatctctac
     3601 gaactccatc gatgattacc aagttgtgat ggcagaatat aattaattga tagaggagaa
     3661 aaaagatggg ggaatttttg ttaagcattt ggacgtattt tgcaacaaat atcttgacgc
     3721 agccagcata cctgattggt tttatcgtgt tattaggcta tattttgctg aaacgcccgc
     3781 ttcatgaatg catcgctgga ttcttaaagg caacggtcgg ttactttatt ttgactgttg
     3841 gctctggtgg tttggtcaat aattttcggc caatcttggt tggactaaag gaacggttca
     3901 atttggatgc catggttaca gatccttatt ttggacaaaa tgctgtggat gctggattaa
     3961 tggagacatt tggccgtacc tttggagatg tgatgatttt attactgatt gcatttgttt
     4021 taaatatctt attagtccgt tttcagaaat atacgaaact gcgttccgtt ttcactaccg
     4081 gaaacgttca ggttcaacaa gcagctaccg ctttttggat tttactgttt tgctttcctg
     4141 atctaggtag gatacaagca ttgattttta tgggactgat tttaggttgt tattggacag
     4201 tagcttctaa cttaacagta ggaatcacgc aggaattgac agaaggtgcc ggatttgcag
     4261 tggcacatca gcaaatgttc ggaatcttta tatttgcaaa attagctgag tggatgaaaa
     4321 agcgggatga aaagaaaaat agatcaatca agcaagacaa aaaaattgaa gatatcaaat
     4381 taccaggatt cttgtcgatt ttcaatgaga atatggttgc tacttcgtta ctcatgctat
     4441 ttttctttgg gatcattctg attgtactag gcaaagatta tttgattcaa gcgcaattca
     4501 tgcaagaagg gcagagtttc ttattctaca ttatgacgac ttctttaaat tttgcggtat
     4561 atctggcgat cttgcaatta ggtgttcgga cattcgtcga tgaactaact caatctttcc
     4621 aaggaatctc aaacacgatt ttaccaggag ctgttccagg aattgacgta gctgctactt
     4681 ttggctttgg ttcgccaaat gcggtaacga ttgggttctt gtttggtgct ttgggacagt
     4741 ttttgatgat tggtttactg attttattga aatcaccaac gatcgtcata gccggtttca
     4801 ttccgttgtt ctttgataat gccgttatag cagtttatgc caataaccga ggtggattca
     4861 aagccgcttg cctgttcccg tttctttcag gaatcataca agtaggcggt tcagcattat
     4921 tcgctacatg gattgggatg tcaacatacg gtggttatat cgggatgttc gactgggcaa
     4981 cagcttggcc attgtttaca gtaatcatga aagtcctagg ttatgctgga attgccgtag
     5041 ttgtcattgg tttattggct attccgcagt tacaatatcg gaaaaatcct gatggttatt
     5101 ttttgattgt agaagactat gacacatatg cagaaaagca gagagtataa aagaaaatct
     5161 gattcgacaa cactcataat ggagggatta agatgagaat tttagtatcg tgtgcaaatg
     5221 gatcaggaac aagcttgatg atgatgagaa gtgtggaaaa agcaatgaaa gaactaaacg
     5281 taccaattac caaaatccat cattgtgcca tttcagaggg aaaaagttca gctggccaat
     5341 acgatgtagt ttttacgcca gtcaattttt tgaatatgtt tactcaggca gagaaaaaag
     5401 gagttacagt tatcggtatc aaaaatgtga tgtcagtcaa agaaatcagt gaaaaatttg
     5461 ctgaaacaaa gtggtaccaa aaaaacaagt aaaggaagga actaagatga gcaggccgaa
     5521 tttgcaaatt gcgttagatc acaatgactt agaacatgca ttaggagatg ttatgaaagt
     5581 tggtgatgta gtagatatta ttgaagtggg cacaattctt tgcttacagg aaggacaaaa
     5641 agcgattcgc tgtattcgca gtatgttccc agataaaaaa ctagtcgctg atacgaaatg
     5701 tgcagacgct ggaggaacag tggcgagtaa tgttgcaaaa gcaggagcag actggatgac
     5761 agtcatttgt tgtgcaacga ttccaactat ggaggcagca caaaaagaaa taggtgaatt
     5821 gcaagtagaa ctctacggca attggacttt tgaacaagcc atggattggc ataacatcgg
     5881 tatccggcag gtaatctacc atcaaagccg tgatgcgctg ttatctggtg aaacttgggg
     5941 tgaaaaagat ttatcgaaga tcaaaaaact gattgaacta ggctttaatg tttctgtaac
     6001 tggtggatta aatccacaca cattacactt atttgaaggg atcgatgtgt atacctttat
     6061 cactggtcgt ggtattacag cagcaaatga tccgatgaag gccgcacaaa acttcaaaga
     6121 tgagatcatt cgtatctggg ggtaaggaaa tggctcaaat tgggatttat gaaaaagcgt
     6181 tacccaaaga tatttcatgg aaagaacgtt tttcccttgt aaaagaaatg gacttcgatt
     6241 ttatcgagat gtcgattgat gagacggatg agcggttagc acgattagat tggtctgaag
     6301 aaaagatagc tgagctgaga gaagagatgt tcagctccgg tgtacgtatc cattcgattt
     6361 gtttaagtgc gcatcggcga tttccgtttg gttctgctga tccagaaaag aaaaaagcag
     6421 cgaaacaact aatgaaaaaa gcaatccatt tggcgcataa tctaagcgtc aaagtaatcc
     6481 aaatagctgg atacgatgtt tattatgaag agaaatcaat gaattccaga gaagacttta
     6541 tcgaaggaat aaaagaaagc gtaaaggaag cttctaaata tggcatcatt ctttccgtgg
     6601 aaatcatgga tgatccattt atgaactcca tttcaaaatt tttagaaatc aaagaacaga
     6661 ttcattcacc ttttctacaa gtatatccag acctaggcaa tttgtccgca tggccggaaa
     6721 ataatccggc tcgagaactt gagaaaggga tcgattatat cactgctatc cacttaaaag
     6781 atacttaccc tgtaacaaaa gaatattcag gccagtttcg tgatgttcct tttggtagcg
     6841 gttgtgttga ttttcttgga ttattaagac atttgaaacg tctagattat gatggaacat
     6901 ttctgattga aatgtggagt gaaaaaagcc aagactttcg aagagaaatc cagcaggcaa
     6961 aagactactt aagttctaaa ttgaaggagg ctggctatga tgtcttgtga agaaaaaatt
     7021 gcagcaatga agcaacgtgt ccttgaagca aatctggcat tgccgcgata cggattagtc
     7081 aaattgactt ggggcaatgt gagtgaagtg gaccgtgagc taggagtcat cgtcattaag
     7141 ccaagcggtg tcagatatga atgcatgcaa gcagatcaaa tggtggtaac agatttgtcg
     7201 ggaaatataa tagaagaaga tagcttaaaa ccatcttcag atctccccac acatgttgtt
     7261 ttgtatcaaa cttttgaaga tataactgct attactcata ctcattccac gcactccgtc
     7321 atgtgggcgc aagcaggacg cgatttgcca gcttatggca cgactcatgc tgatgctttt
     7381 tatggtaagg ttccttgtac acgccaatta acgaaagaag aagtaagaga agcttacgaa
     7441 gttcatacag ggaatgtgat tgtcgagacg tttaaggaaa gaaaattaga tccaaacgaa
     7501 gtccctggag tacttgtcta tggacatgga ccgttcacat ggggggattc acctatgaaa
     7561 gcagtagaaa acagtttgat cttagatgaa atttgtctga tggccaaaga aaacgagctg
     7621 atcaacccca atatatgtga aatcccgcaa tatttactag ataaacatta ttttcgcaaa
     7681 catggaaagt atgcatacta cgggcaatag agatgctagg gtaagggtag aaaattaaaa
     7741 gataacactt gatagagaga aacagaggtt gtgaagaaaa tttatcacaa cctttttatt
     7801 tatcaaaaag tatgcattct aaaaggaaag aggaatttta aggaatcgag agaatcttta
     7861 caggagagtt atatgtgata gattaaatag tcaaagaact gtattattag agggagaatt
     7921 gtataagaaa ggaagaaaaa aatgaatatt gattccctta tgatcgatga gatttctgaa
     7981 ttatataaga gcgttggttg gacgaattac acaaaagaca ctgcaaggtt agctagagca
     8041 tttgaacaat ctgaatctct gataaagcgt aatggggaag ggaagattat cggaatcgtt
     8101 cgttggatca ctgattttgc gacgatcgca tttattcaag atatcctcat tcatccgagg
     8161 tatcagcgcc aaggaatagg caaagtgctt ttgaatgaag ctttggagaa gatcactagt
     8221 tatggaccag tccaaatcga attattgaca gatgatactg agaaaacaaa aaaattttat
     8281 gaatcagtag gatttgttca agtaaaggaa atgggtggta tttcttatgt catagatact
     8341 agaagaaaaa aggtgc
//
LOCUS       DAXWOO010000125         8296 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.127, whole genome shotgun sequence.
ACCESSION   DAXWOO010000125 DAXWOO010000000
VERSION     DAXWOO010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.127"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(<1..187)
                     /locus_tag="ACGE70_002166"
     CDS             complement(<1..187)
                     /locus_tag="ACGE70_002166"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693784.1"
                     /translation="MSENKKKWLLIHPFDYISRTNILEEILTENSIEYFNVVSDYSHI
                     RKQFLSSKERHMIKNRHV"
     gene            complement(180..809)
                     /locus_tag="ACGE70_002167"
     CDS             complement(180..809)
                     /locus_tag="ACGE70_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFF8693785.1"
                     /translation="MSNRGIYEKFFKRLIDILFSILGLIMLWPLMTFVAILVRIKLGS
                     PVIFKQQRPGKHEKIFLMYKFRTMTDQRDEEGNLLSDDIRLTQFGKILRSTSLDELPE
                     LWNILKGDMSIIGPRPLLVEYLSLYSNEQRKRHFVRPGLTGLAQVSGRNAISWKEKFE
                     KDIEYVENISMMKDIKIFFLTLKKVIVRDGITSVASGTTEKFEGAKIDE"
     gene            complement(802..1920)
                     /locus_tag="ACGE70_002168"
     CDS             complement(802..1920)
                     /locus_tag="ACGE70_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFF8693786.1"
                     /translation="MENKRILLASPHMSDEGYEQEYVKEAFDTNWVAPLGMNVNGFEE
                     ELAEYVGASHAAALSSGTAAIHLALKAAGVGKDDIVICQSLTFSATANPIIYQGAIPV
                     FVDSDYETWNMNPEHLRTALEKYPNAKAVLVVHLYGLSAELDEITKICKEHNVVLIED
                     AAESLGTTYKGKYTGTFGDYGIYSFNGNKIITTSGGGMLVSNDKEKVEKVRFWATQSR
                     EKARHYQHNEIGYNYRMSNIVAGIGRGQLKVLNERIEKKRYIHSFYKKELSKLSNIQF
                     MPENNWNEPNYWLSVITVKGINTNHLIDELDKNNIEARPIWKPMHLQPVFDKYEYIGT
                     DVAQTLFEKGICLPSDSKMTDDDLYRVTNTIKRVMQNV"
     gene            complement(1922..2554)
                     /locus_tag="ACGE70_002169"
     CDS             complement(1922..2554)
                     /locus_tag="ACGE70_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFF8693787.1"
                     /translation="MKAIIVGAGGFGKEIAFLLESFSRYELIGFVDDSLKMQNQELVG
                     KTVLGTIDSLLELEEETAIFLGIASPDIKEKIYQKINKNNKLIFPNLVASSALVGINV
                     QLGIGNILMPYTTYTADVVLGNFNMINIGSTIGHDTQIGNYNSIFPSVNISGHVLLGD
                     KNEIGVGTKIIQNLRIGNMNIIGAGSVVIKNINNKTKNVGVPTKVIERWD"
     gene            complement(2565..4382)
                     /locus_tag="ACGE70_002170"
     CDS             complement(2565..4382)
                     /locus_tag="ACGE70_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFF8693788.1"
                     /translation="MFVLTRRRKIATLVFVDSLLLIAANIAAAKFMKPFVAIPMDLIL
                     ISIGLSIGFYLFYGSLFKVFTRINRYTNLREIIAIFGSLSASAASSIIILLFINKQYS
                     LRLVIFAYLLSLLLIIGSRLIWRIYIETKNMRYLSTDSAKNTLIVGAGEGGRILYNSF
                     LGSKTAQDIHVIGFVDDDPNKRNTYLSGKKVLGSLKDIPELIEKYDIQMVTIAIPSLS
                     RKKLRRIFELVESAHVKVNTMPSMEELASGKISVSKLKTIDVVELLGRDEVELDIESI
                     KDQITNKVILVTGAGGSIGSEICRQIIQFDPAKLLLLGHGENSIYLIDRELRTHHQKC
                     PTEIVPIIADIQDRKKINEIMEQYHPDIVYHAAAHKHVPLMEYNPKEAVKNNIFGTKN
                     VAEAAKAAKVKNFVMVSTDKANNPPNVMGSTKRIAEMIVTGLNEEGCTKFSAVRFGNV
                     LGSRGSVIPVFREQIAQGGPVTVTDFRMTRYFMTIPEASRLVIQSGALAKGGEIFILD
                     MSEPVKIVDLAKNMIRLSGYSEDEIEIIETGIRPGEKLYEELLLDKERNDEAVYEKIF
                     VGNIKGYSMQTVMEFVKSLPQDDEQLAKDIVTFANASNK"
     gene            complement(4395..5159)
                     /locus_tag="ACGE70_002171"
     CDS             complement(4395..5159)
                     /locus_tag="ACGE70_002171"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFF8693789.1"
                     /translation="MIDLHCHILPGIDDGAENIDASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEWLFFELLQLGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKMMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVAQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(5187..5885)
                     /locus_tag="ACGE70_002172"
     CDS             complement(5187..5885)
                     /locus_tag="ACGE70_002172"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFF8693790.1"
                     /translation="MARTQKQKATQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSRDAGYYYGN"
     gene            complement(5897..6676)
                     /locus_tag="ACGE70_002173"
     CDS             complement(5897..6676)
                     /locus_tag="ACGE70_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFF8693791.1"
                     /translation="MEETISLKEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGSAPARPKATNDKTAPSRRSRSRV"
     gene            complement(6692..7636)
                     /locus_tag="ACGE70_002174"
     CDS             complement(6692..7636)
                     /locus_tag="ACGE70_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFF8693792.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTLQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(7682..>8296)
                     /locus_tag="ACGE70_002175"
     CDS             complement(7682..>8296)
                     /locus_tag="ACGE70_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693793.1"
                     /translation="SKEVDKLYSTKEKNFLNPEITEETITSLSNKLSSVKLSADDYDI
                     KESELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVVIKE
                     KVTSADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTA
                     TATYDQYLTALSQIEQIRNEKISAAYATKAQTVAQQMGYSNISY"
BASE COUNT         2212 a         1730 c         1413 g         2941 t
ORIGIN      
        1 tcacatgacg atttttaatc atatgccttt ctttactgga tagaaattgt tttcttatat
       61 gtgagtaatc agatacaaca ttaaaatatt cgatactgtt ttccgtcaga atttcctcta
      121 atatattcgt tcgactgata taatcaaaag gatgaataag cagccacttt tttttattct
      181 cactcatcta tcttcgcacc ctcaaatttc tcagtagtcc cacttgctac tgatgttatt
      241 ccatcacgga caataacttt ctttagcgtt aaaaaaaata tttttatgtc tttcatcatt
      301 gaaatgttct ccacatattc aatatctttt tcaaattttt ctttccaact aattgcatta
      361 cgaccactga cttgagctaa tccggttaaa cctgggcgca caaaatgtct ttttcgttgt
      421 tcattactgt atagtgaaag atactcaact aacagaggac gcggtccaat aatactcata
      481 tctcctttta aaatattcca taattctggt agttcatcaa gactagtcga acgcaatatt
      541 ttaccaaatt gagtcaatct gatatcgtct gataataaat taccttcttc atctctttga
      601 tctgtcattg ttcgaaattt atacatcaga aaaatctttt catgttttcc aggtcgttgt
      661 tgtttaaaga tcactggcga tccaagcttt attcgaacta ggattgcaac aaacgtcatc
      721 agtggccata acattattaa acctaaaatt gaaaaaagaa tatctatcaa acgtttaaag
      781 aacttttcgt agatacctct attagacatt ttgcatcact cgcttaattg tattagttac
      841 tcgatacaaa tcatcatcgg tcattttaga atctgaaggt aaacagatac ctttttcaaa
      901 caatgtctga gcaacatctg taccaatata ctcatactta tcgaaaactg gctgtaaatg
      961 cattggtttc catatcgggc gggcttctat attattttta tctaactcat caataagatg
     1021 attcgtattt attcccttta ctgttatgac acttaaccaa taatttggtt cattccaatt
     1081 attttctggc atgaattgta tatttgacaa tttagacaat tcttttttat aaaaactatg
     1141 aatatatcgt tttttctcaa ttctttcatt taaaactttc aattgtcctc gaccgatacc
     1201 tgcaacgata ttgctcattc gataattata gccaatttca ttgtgttgat aatggcgtgc
     1261 cttctcacga gattgcgtag cccaaaacct cactttttcc accttttctt tgtcattaga
     1321 cacaagcatt ccgccacctg aagtcgtgat tatcttattt ccattaaaag aataaattcc
     1381 atagtcgccg aatgtaccag tatattttcc tttatatgtt gttcctaatg attctgcagc
     1441 atcttcaatt aaaacaacat tatgttcttt gcatatttta gtaatttcat ctagttcagc
     1501 tgataaaccg tacaaatgca ctactaatac agcttttgcg ttaggatatt tttctagcgc
     1561 tgttcgcaaa tgttcagggt tcatattcca agtttcgtaa tcactatcaa caaatactgg
     1621 aatagctcct tgatagatga ttggattggc agtagctgag aacgtcaaag actgacaaat
     1681 cactatatca tctttaccga ccccagccgc ttttaaagct agatgtatag ctgctgttcc
     1741 agaagatagt gcagctgcat gactagcacc gacatattct gctaattcct cttcaaaacc
     1801 attgacattc atacccaaag gagccaccca atttgtatca aatgcttctt ttacatactc
     1861 ttgttcatat ccttcatcac tcatatgagg agatgctaat aatattcgtt tattttccaa
     1921 cttaatccca tctttctatg actttggtag gtacgccaac atttttagtc ttattattaa
     1981 tattttttat tactacactt cccgcaccta taatgttcat atttccaatt cttagatttt
     2041 gtattatttt agtacctact ccaatttcat ttttatctcc taatagaaca tgtcctgaaa
     2101 tatttacact tggaaaaatt gaattataat taccgatttg agtatcatgt ccgatagtag
     2161 agccgatatt gatcatattg aaatttccta aaactacgtc agcagtgtaa gtcgtatagg
     2221 gcatcaaaat attgccaatt cctaactgaa catttatgcc cactagtgca cttgatgcaa
     2281 ctaaattagg gaaaatcaat ttattatttt tatttatctt ttgatatatt ttctctttga
     2341 tatctggcga agcgattccc agaaaaatag cagtttcttc ttctaattct aataacgagt
     2401 ctattgttcc caatacagtt tttcccacta attcttgatt ttgcattttt aacgaatcat
     2461 cgacaaaacc aatcaattca tatctagaga aactttctag tagaaaagca atctctttgc
     2521 caaatccacc agctcctaca ataattgctt tcactttact ccccctactt attcgaagca
     2581 ttcgcaaaag tgacaatgtc tttagccaat tgttcatcgt cttgcggcag gctcttgaca
     2641 aattccatca ctgtttgcat ggaataacct ttgatgtttc ccacaaagat cttctcgtaa
     2701 actgcttcat cgtttctttc cttatctaac aatagttctt catatagttt ttcccctgga
     2761 cggatacctg tttcaatgat ttcgatctcg tcttcgctat atccgctcaa tcggatcatg
     2821 ttttttgcta gatctacgat tttcactggt tcactcatat ctaaaataaa gatttcgccg
     2881 ccttttgcta acgccccaga ttgaataact aaacgacttg cttcaggaat cgtcatgaaa
     2941 tatcgggtca tacgaaagtc tgttacagtg actggccctc cttgagcaat ttgttcccgg
     3001 aatactggaa tcacactacc gcgagaacca aggacattcc caaatctgac agctgagaat
     3061 ttcgtacatc cttcttcatt caacccagtc acgatcattt cagcgattcg ttttgttgaa
     3121 cccatgacgt ttggcggatt gttggcttta tctgtcgaga ccatgacgaa gttttttact
     3181 ttcgctgctt tggctgcttc tgctacattt tttgtaccga aaatattgtt tttgactgct
     3241 tcttttgggt tatattccat caatggtaca tgcttatgag cggctgcatg gtagacgata
     3301 tctgggtggt attgttccat gatctcattg attttcttgc gatcttggat atccgcaatg
     3361 atcggcacga tttctgtagg acacttttga tgatgcgtgc gtaattcacg gtcaatcaag
     3421 taaatcgagt tctccccatg acctaaaagt aatagttttg cagggtcaaa ttggatgatt
     3481 tgccggcaga tttctgaacc gattgatcct cctgcaccgg tgacaagaat gactttgttt
     3541 gtgatctggt ctttgatgga ttcgatatcc aactctacct catctcttcc cagtaattcc
     3601 actacgtcga tggtttttaa ttttgatacg ctgattttcc ctgaggcaag ttcttccata
     3661 gaaggcatcg tattgacttt cacatgagca gattcgacta actcgaagat tcttcgtaat
     3721 ttttttcttg ataaagaagg gatagcgatc gttaccattt ggatgtcata tttttcgatc
     3781 aattccggta tatcttttaa tgaccccagt actttttttc ctgaaagata ggtatttctt
     3841 ttattcggat catcatccac gaatccaatg acatggatat cttgagccgt tttagaacca
     3901 agaaagctgt tgtataaaat acgtccacct tcgccggccc caacgatcaa ggtattttta
     3961 gcggaatctg ttgatagata cctcatattt tttgtttcga tatagatacg ccagatcaat
     4021 cggctgccaa tgatcagtaa tagcgaaagt aggtatgcaa agatcactaa acgtaaactg
     4081 tattgtttat ttatgaaaag caatatgatg atacttgagg ccgcacttgc agataacgaa
     4141 ccaaaaatcg caatgatctc tcttaaattt gtataacgat tgattcgagt aaaaactttg
     4201 aataaactac catagaataa ataaaaacca atcgacaaac cgatagagat taaaataagg
     4261 tccattggta tagctacaaa cggcttcatg aatttagctg ctgcaatatt cgcagcaatc
     4321 aacaacaagc tgtccacaaa taccaatgtg gcaatttttc ttcttcttgt taatacaaac
     4381 accctacatt ccccctaaaa taaaccaaac ttcttctttt ttacctcttc atagactgga
     4441 taactgatct catccccgtt gatcaggtcc tttgcgactt gtttcatctg tgctactttt
     4501 tccttaccaa agtccttggc gatttgctca taacattctt ttaagtaaaa ggtacgttta
     4561 gagaccccat gggcatcgga agcagccatt tgcacgaggt tatgtttgac catcattttg
     4621 gctgttttct ggatgtcttt tccaaataca ccgacataac ttggtgcggt gagttgagcc
     4681 aagcatccca tatctaggaa tggcagcaaa tgatttgggt cttctcgaaa atgcgcgttg
     4741 cgttctggat ggacgatcac tggtgtcttt cctagttgta acaactcaaa aaagagccat
     4801 tctgcatata acggcacttc cagtgtcgga aattcgatta gaagatatgt atcatctaaa
     4861 tctgtaaata aaatttcatc tcggtggatg tcttcaatca gtgttccggt aatacgtact
     4921 tcttgtcctt ccaataacgt caaaggcaat tgtcttttct ctaattctgc ttgtaacgaa
     4981 gcgactagag aaatgacttg tgatttttca ttgctgtatt ttccattatt gtgatggggt
     5041 gtgcataaaa tatgtgtgat cccttgttga atcgcttttt cagccatggc gatactggca
     5101 tctatgtttt ctgctccgtc atcgattcct ggcaggatat ggcaatgtag atcgatcatt
     5161 tctcttgtct cctctctttt tatttcttaa ttcccgtagt aatagccagc atctctgctg
     5221 tgttctgctc cattgtatac aacaccaagg actcgcgctt gtaccatatc taacagttct
     5281 tttgctttag tcaatgattc ttttcgtgac gtattttccc gtacgaccaa taaggtgcca
     5341 tccgtttttg aagacatgat ctgtgcatct gttacggcaa cgactggtgg catgtcgaaa
     5401 atcactacat caaatagttg tcttgcttct gctaaaatct gatccattct tggcgaactt
     5461 agtagttctg atggatttgg tggtttaggt ccacttggca gaatcgacaa attgtcgact
     5521 ggtgttcgtt ggatctcatc tgctacactt cctgatgaac taagaacagt acttaatccg
     5581 cttgcgttgt tcaacttgaa cgttttatag atgacgggct tacgtaagtc tgcatcgact
     5641 agcagtaccc gttgaccaga tttggcaaaa acaacggcaa tgtttgctgc agtggtggat
     5701 tttccttctc caggacctga tgaagtcacg acaatcgttt tgatttgctg accagctgcg
     5761 gaggcaaact ggatattcgt ccggatcgta cgatattgtt cggcgattgg tgaagatggg
     5821 tttgttaatg tgatcaaact gactgcatga gtttgagttg ctttttgttt ctgtgttcgt
     5881 gccatttttc ttcctcctag actcttgagc ggcttctgcg tgatggggct gttttatcgt
     5941 ttgtagcttt tggtcgtgca ggagctgatc cttttttacg gataccagcg ttcaattctt
     6001 ttgctgacat ttctggaact gttcctaaga ttgggaatcc taatgtatcc gtcacatatt
     6061 tttcatcttt cactgtccga tcaagtaatt cgaataagaa tgccagacct acgcctacca
     6121 tcattcctaa cactaagcca atcactagat tcaatttatt atttggcgaa acaggtgtcg
     6181 tattggcaac tgcacctgaa ataatcgaga ttttatcgac attcatcacg tctttggcgt
     6241 tttcttggaa aacttgtgaa gttgtattag cgatctgttg agcagtcact gcattcgtgc
     6301 ttattgcctg gatcgagaac atttgtgaat tttgtgactg attgacggag atcgcatctt
     6361 tgatttgtcc agcagtcatt ttcacattgt cttctgtttc taaacgatct ttgacttcat
     6421 ttagtactaa atcactgacg atcatatctt tatacgtatt gatcatttgc aggttcgtat
     6481 tgacatcatt gacatttgct gtgtctgatt gtggcagtgt cacgatcaat tgtgcttgtg
     6541 aactgtattt tggtgtcaag atgaaaaatg ttacgattcc tgagatggcc aagccagcaa
     6601 acattgagat gatgattgtt accaagtgtt tgcgtaatgt ttcaaaaatt tctttcaagc
     6661 tgattgtctc ttccattttt tctccacttt cctatttatt ctctagattc atttgatctt
     6721 ttaattcttt tttcacacga tcaagttctt gatcgctgac tttttgatag gagatattat
     6781 cttgcatgaa gccttctcct tgcagctgat cttgcttgat cgtaccaaat gcctcccgat
     6841 aatctaaagc gatttttttc atttcatcaa agctcatgtc tgttttcata ttcgactcta
     6901 gagcattcaa aacggattga tagttgctga caccgtttaa actcaatact ttttttgcta
     6961 caccttctac gatctttctt tgacgctctt gtctaccata gtcaccattt ggatcttcat
     7021 aacgcatacg agaataagat aatgcctgat ccccgttcaa tgtaatttta ccgattggaa
     7081 agtcatagcc atcttgtgag aaagtgaggt cattattgac ttcgatacca ccaactgcat
     7141 caacgagttc ctttagccct ttcatattga tagagacata gtgatcgatt gggatgtcta
     7201 catatttttg tagggtatcc atcgccatgg cagctccgcc gaatgcgtaa gcgtggttga
     7261 tcttgtcttt tgtgtcacga cctacgatgt cgacatacgt atctcgagga atgctgacga
     7321 tcgttgtctg tttgtcttca ggactgactg tggcaaccat catcgtatct gatcttcctt
     7381 gatcgacccg acctaaatct ccagtatcaa tgcccatcaa tagaacagag aaagattctt
     7441 gtttgtccag atcaactggt ttcgttcgtt ctccttgggt tcgttccact gattcgtaag
     7501 ttttctcaat agaatgtgag agatccagat ataattttgc tcctactcct acaattgcta
     7561 atactaatac gataagaact gctaaaataa tcagaaatat ttttttccct cttgattttc
     7621 ttcttcgtct tgccatagtt tcctccgttt gctaaccttg cttgaatcaa cgttttaact
     7681 gtcaataact tatattgcta tagcccattt gctgagcaac agtttgtgct tttgttgcgt
     7741 atgctgcgct tattttttca ttccggatct gctctatttg actgagcgcc gtcaaatatt
     7801 gatcgtaagt agcggtggca gtcacttgac catccttcaa catcgtatcg aataactgat
     7861 ctagttgcgt gacttgcgcg atctgagtat cagcgaatcc tagatattgc ataacaaccg
     7921 ttttccaagg actatcttta aaaaagctca tattttcacg tacacgagcg acatctgcac
     7981 ttgtcacctt ttctttgatg acgacatcgt ctacagcttg ctgccagttt gatacatttt
     8041 tagtaaataa atcgttgaca gctgtctgga tcttcagttt tgattctgca ctatctaact
     8101 gtgtcacgat tgctttcttc tcatcttgga ttgcaacagc ttctttaggc aattctgatt
     8161 ctttgatgtc gtaatcatct gctgaaagtt tgacagagga tagcttattt gacaaacttg
     8221 tgattgtttc ttctgttatt tcagggttta gaaaattttt ttcttttgta ctatacagct
     8281 tgtctacttc ttttga
//
LOCUS       DAXWOO010000126         8147 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.128, whole genome shotgun sequence.
ACCESSION   DAXWOO010000126 DAXWOO010000000
VERSION     DAXWOO010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8147
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.128"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     regulatory      1..78
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            76..1269
                     /gene="metK"
                     /locus_tag="ACGE70_002176"
     CDS             76..1269
                     /gene="metK"
                     /locus_tag="ACGE70_002176"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFF8693794.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1399..2871
                     /locus_tag="ACGE70_002177"
     CDS             1399..2871
                     /locus_tag="ACGE70_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFF8693795.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            2954..3742
                     /locus_tag="ACGE70_002178"
     CDS             2954..3742
                     /locus_tag="ACGE70_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFF8693796.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(3809..4477)
                     /locus_tag="ACGE70_002179"
     CDS             complement(3809..4477)
                     /locus_tag="ACGE70_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFF8693797.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            4592..6304
                     /locus_tag="ACGE70_002180"
     CDS             4592..6304
                     /locus_tag="ACGE70_002180"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8693798.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6307..8079
                     /locus_tag="ACGE70_002181"
     CDS             6307..8079
                     /locus_tag="ACGE70_002181"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8693799.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2488 a         1373 c         1798 g         2488 t
ORIGIN      
        1 tcgttgcaag ttcccgaaag gatccgcagg acggagatgc cttgtaaccg aatttaacta
       61 gggggaatca ttataatggt agaaagacac ttatttacat cagaatctgt ttctgaagga
      121 catccagata aaattgctga ccaaatcagt gatgcaatct tggatgcaat tttaaaacaa
      181 gatccaacag cacgagtagc gtgtgaaacg tctgtaacaa ctggtcttgt tttagtattt
      241 ggagaaatat ctacgacggc atatgtagat attcaaaaag tcgtacggga aacaataaaa
      301 gaaattggtt atacacgtgc aaaattcgga tttgatggag atacagcagc tgtattggtt
      361 gcgattgatg aacaatctcc tgatattgct caaggagttg atgaagcact tgagatccgg
      421 gatgaagata aaaaagacgt attagatgag attggtgctg gtgaccaagg tttaatgttt
      481 gggtttgctg tagatgaaac accagaattg atgcctttac caatcgcttt gagtcatcgt
      541 ctggtacgac gcttggcaga tttgcgtaaa tcaaatgaat taacgtattt acgtccagat
      601 gcaaaatctc aagtaacggt tgaatatgat gatcaaggac aaccggaacg cgtagataca
      661 atcgttattt caacacagca tgatgatgca gtagataatg aaacaattcg tcatgatgtc
      721 atcgaaaaag tagtgaagga agttattcca gctgaattat tagatgatca aacgaaatat
      781 tatatcaatc cgactggccg atttgtcatt ggtggtcctc aaggggatgc cggattaaca
      841 ggaagaaaaa tcattgttga tacgtatggc ggttatgctc gtcatggtgg cggtgctttt
      901 tctggtaaag atgcgacaaa agttgaccgt tctgccagct atgctgcacg ttatattgct
      961 aaaaacattg ttgcagcagg gcttgctcgc aaagcagaag tacagctagc ttatgcgatt
     1021 ggtgttgctc aacctgtttc gatctcaatc aatactttcg gtactggaac ggttccggaa
     1081 gaagaactga ttgctgcagt gagagaaaac ttcgatcttc ggccagcagg aatcatcgag
     1141 atgcttgatt tgcgtcgtcc gatttacaaa caaacagctg cttacggtca ttttggtcgt
     1201 acagatgtag atttgccttg ggaacatacg gacaaagtag atgcgttgaa agcaagttta
     1261 gcaaaataag ttatattcag ctggaacgat cgccaagtgg tggttgttcc agcttttttt
     1321 atggcagtta acctcttgaa aaagatagta attatttatc tatttttaga tcctgtcata
     1381 atgaaaggga ggaataagat gaaaaaagaa acaaatgtta ctttggtcac agtgagtatt
     1441 ttcattgcaa cgttcatgac ggctattgaa ggtacaatcg taacaaccgc tatgccaacg
     1501 attgtcggtt cattacatgg aatggaaatc atgaactggg tattttcgat ttatttgctg
     1561 acaaacgcta tgcttactcc gatttacgga aaacttgcgg ataaagtggg acggaaacca
     1621 atctttattt ttgggacatt attgtttatt atcggttcgt tattttgcgg tttatctcaa
     1681 agcatgattg ggttgattgc tgctcgtgcg attcaaggga tgggtgctgg ggcaatgatg
     1741 ccagtagctt tgacgatcat cggcgatctt tattctattg aaaagcgagc aaaagtttta
     1801 ggtttgaata gttcagcctg ggggattgcc agtgtttttg gaccgcttgc gggaggattc
     1861 atcgttgaaa cgatcagctg gcactggatc ttttttatca atgtgccgat cggaattcta
     1921 ttaattattt tgatttggct atatttaaat gaaccaaaaa tcatacatga gtctaaacca
     1981 atggatattt ttggtagttt tagtttgatg gccatgcttg tctcattgct gttagggttc
     2041 caattattag gagacgaagg aatgagtccg cttgtttctg ctttgctgct aggtagtatc
     2101 gtaatgttag gtatttttgt ttttgccgaa aaaagagcta aagatcctgt catcatgatg
     2161 catttattca aaaatccact atttgttgtg gtcaacttgg ctgcagcatt ggctagtggt
     2221 tttttgatgg gaatcgatgt ttatatccct atgtggatgc aaggtgtatt agggctgaat
     2281 gcagcaattg gtgggctggt attagcacca ttatcactag tctggatggt cggttctttc
     2341 cttgcaagca agtggatgac tcaaatgaaa actcattggg tcttgagaat tggactacta
     2401 atcgcagcta ttgggggcgc ttggttgtat cttatgcctt tcagaagtaa ctatttatgg
     2461 ttttttgcta tatcaacggt catcggtata ggcttaggtg tgaccatgac tacctcgact
     2521 gtcagtgcac aaaatagtgt atctcgtgac cagcttggtg tagcaacttc ttttaatacg
     2581 ttagttcgaa caattggtca aacagtcatg gtttctattt ttggtttatt gataaatggg
     2641 gtaactaaac gagaacttgc agcagcaaat ttaacaaatg atgcagatat catgaataaa
     2701 ctggtgaatc cacaaacggc taaatatatt gatgcggata ttttgttacc attaagaaaa
     2761 attctttatg atgggttaca ttccgtttat ctagttggct tgttactcgt gatcattgct
     2821 ttggtcttaa cgcttttcgt tcatgaaaaa agtggtaaaa taaaacagta gtttcggtta
     2881 agatatgtac gtagccgaat cattcaaatt taaagttatt caaaaataac ttcttactaa
     2941 ggaaggcgaa aatatggcat ttattcaagc aaatatttat tcgaatgtgt tagaaatgga
     3001 agtcaatcta aatgtgatac taccgcaaac aacaaaaaag gtgattggca ccagttcaga
     3061 gcaagagaca acggatatac ctgttttata tttacttcat ggaatgggtg gcaatcatag
     3121 tgtatgggaa agacgtacgt cgattgaacg ttatgtagca aactacggat tagctgtcat
     3181 catgccatct actgatcttg gctggtacac agatacagtt tatgacatga agtattggac
     3241 attcattgca gaagagctac ctgagatttg tcatgaatta ttcccgcaat taacaagaaa
     3301 aagagaaaaa acatatgccg ctggattatc gatgggcgga tatggcgcgt taaaactagg
     3361 ccttgcaaaa tcagaaaatt tcgcagcagt aatctctctt tcaggcgctg tttctgtagg
     3421 aaatcgaatg gatgatcttt tgatggttcg taaagcatct ttttgggaag ggatctttgg
     3481 accattggat caaatcgaag gttcaaaaaa tgatccgcaa tacttattga aagaactagt
     3541 aaaaagcgga aaaccagttc caagatttta tatggcttgt ggggaatctg attttcttta
     3601 tcaggcaaat caggaaatgg tatctgctat gcaggaacaa ggcttggcgg tcacctttga
     3661 acaaggacct ggcgatcata actggatctt ctgggaccaa tggattcaac gtgcattggc
     3721 gtggctaatg gaagaaaaat aaaaaaagag tcttaaaaag atagctccta tagagatcac
     3781 aaacatagtg atctctatag gagctttttt actgctcgtt ttctcttctt ttattttgtt
     3841 ggcataagcc ttcaatatag gtttgtgcac tcgttagaaa gagtgctttt tcattcaatt
     3901 ctgcatggga ctgcatactt tctttaaatc cctctaattg attttcaact acataattac
     3961 gcataaatag aactgtttct ttcaaaaatt catttccgtc cataattttt tgtcgaagct
     4021 gctgttcatg ctgctgatcc attaactgtc caatcaataa attgtggaga gaggcaatgg
     4081 cctgatgact ttgggtgact gaaaatcctt cacgaacaag taattggatc atttgctcca
     4141 aatgttctaa tcgactcgga aaagcgggca cagtattgat ttctaattct gccccacaag
     4201 ggaaacgtgt ataaaggtca tagtaatttt ccatatactg cagcaactgt tcattccaag
     4261 gcaaagttgg atctggaagc gctaattctt cttccattgt ttcagccatt ttttgcaaga
     4321 gctgttgttt gtttttgaaa taccaatata aagcaggtgc ttgaacatga attctgtttg
     4381 cgatgtttcg catggaaagc tgcgaaagag cttgtgtttc agccaggata tcaaaggcgg
     4441 cctgaatgat gattgcttga gaaagcttta tttccatttt atcaacctcg tgtattgcat
     4501 tatcttttta aacatggtaa attgttagtc ggtctaattt tacagtgtta aattagtcct
     4561 gtcaatttaa acaaaggggt gtaatcaacg aatgaaatta atcttacgac atgcgaaaaa
     4621 atataaagta gctgttttta tttccttgct gtccgtagca gtcatggttg cagcagcatt
     4681 atggcaacct aagttactac agcaagtatt ggaagctatt attacagaag ataatgatga
     4741 aatgaaaaca attggtattt acctgatcag tctggctttg ctaggattag ccgcaggtgt
     4801 aacgaacacg attttctctg caaaagtagc tcaaggcgta agtgcagata tccgagaaga
     4861 agcatttcga aagatccaga cattttcatt tggaaatatc gaacagttct ctgctggaaa
     4921 tcttgttgtt cgtctaacaa atgatatcac gcaaatccaa aatttagtga tgatttcttt
     4981 gcaatcactt tttcgaattc cattcttatt tattggtgcc ttcattttag ctatgatcac
     5041 gatgccgcaa ttatggtgga tcatcgtagc gcttatcgtt acggtatttc tgattacggc
     5101 actttcattt acaaggatgg gcaagcactt catgatcatt caaaaactga ttgatcgtgt
     5161 gaatagcatt gcaaaagaaa acttactagg gattcgtgta gtgaaatctt ttgtacaaga
     5221 agaaaaccaa ttgaagtcat tcagcaaagt aagtgaggac ttaacaaaac acaacatcat
     5281 agtcggtaca ttattttctg tgatgatccc gtccttcatg ctggctgcca atttggctgt
     5341 ggttggggcg atcttttttg tgagcgatct tgttaaagat gatcctacgt taattggtgg
     5401 aattgcttca ttcatgaact acttgatgca gattatgatg gcaatcatta ttggcggaat
     5461 gatgatgatg atgacttcaa gagcagccgt atcgctgaag cggattgcgg aaattttaga
     5521 tgctgaacca gatcttactt atttagacgt tccagaacaa gagttgacgg gttctgtcac
     5581 ttttgaccat gtttcttttc gttatcctgg tgaggataca gatacgctta aagatatcag
     5641 cttttctatc aaacctggag aaatggttgg tatcgtcggg gcgactggtg ccgggaagtc
     5701 aacactagca caattgatcc ctcgtctatt tgatccgaca gaaggcaaag ttgaagtagg
     5761 tggtgtagat cttcgtcaag taaatgaaaa gagtttaaga caaacggttt cctttgtttt
     5821 gcaaaaagca attttattct caggaacgat tgctcaaaac ttgcgccaag ggaaaaagaa
     5881 tgcaacagaa aaagagatgg aacatgcttc ttctattgct caagcaaaag aattcatcga
     5941 aaagttatct gatcgttacg aagcaccaat agaagaacgt agcagtaact tttctggtgg
     6001 tcaaaagcaa cgtttatcca ttactcgtgg cgttatcggt aatccaaaga tcttgatcct
     6061 agatgacagt acgagtgcac tggatgctcg ctctgaaaag ctggtccgag aagcactcga
     6121 tcgagatttg aagggaacaa cgacgattgt catcgcacaa aaaattgctt ctgttgtcaa
     6181 agcagaccgc atcttagtat tggatgaagg cagattagtc ggcgaaggaa cccatgagga
     6241 attggtagct ggaaaccgtg tctatcagga aatctttgaa actcaaaaag gaacggagga
     6301 gtaagtatga ctgatctaat aaaagcaagt aaatttttct atcactattt gaagcggtac
     6361 aaactctcct ttttctttat ctttatcaca gtgatcattg ctacctatct acaagtaaag
     6421 gcacctcaat acatcgggga agcttttcaa gaattagcta attacgtagg aggcttgatg
     6481 caaggcgtag acgataaaag caaatttgtt tctgtaatat ggaaactctt gctgttttat
     6541 gtactagcta gtgcagccaa ctttatctat agtattttgt tcacacaagt agtagggaag
     6601 tcaacgaacc gaatgcgtat cggtctattc aacaaattgg aaaaattgac gatccgtttc
     6661 tttgattctc atcaagacgg tgaaatttta agtcgtttta ccagtgactt agataatatt
     6721 caaaatagtt tgaaccaagc tttgcttcaa gtcatcacga atgctgtttt acttgtcggg
     6781 atcttgatca tgatgttccg ccaaaatgta gagttagctt gggctacaat tgcttccact
     6841 ccagtagccg ttttgatagc tgtcttgatt atccgcaaag cacggaaata tgtggatctt
     6901 cagcaagatg aagtggggaa actaaatggt tatatggatg aaaaaatcag cggacaacgt
     6961 gtcatcatca caaatggctt acaagaagaa acgattgaag gattcttaga gcataatcaa
     7021 agtgttcgcg aagcaacctt caaaggacaa gcttattctg gtctactatt tccgatgatg
     7081 caaggaatgt cactggtcaa tacagcgata gttatctttt tcggcggctg gttagcatta
     7141 aatggtgatt tggaacgttc ggtggcatta ggcttagttg tgacattcgt ccagtattca
     7201 caacaatatt accaaccact gatgcagatt tcatctggat acagcatgat ccaattagcg
     7261 attacagggg caagacgatt gaatgaaata tttgatgaaa aagatgaagt caaaccagaa
     7321 aacggtaaag catttgatgg tgttgaaaaa ggtcttgcgt tgcagcacgt agattttggt
     7381 tattctccag aaaaactagt cttgaaagat gtctcgatca atgtggacaa aggagaaatg
     7441 gttgctcttg ttggtccaac aggatcgggg aaaacgacga tcatgaatct gctaaaccgt
     7501 ttttacgatg tcaatgatgg ttcagtagtc atcgatggaa tagatatccg agattatgat
     7561 cttgatgcat tacgtagaca tgtaggaatc gttttgcaag aatctgtatt gttctccgga
     7621 acgatcaaag acaatatcac attcggcaaa ccggacgctt cagatgaaga agtcatcaat
     7681 gcagcaaaac aagcgaatat tcatgacttc atcatgacgt tagaaaaagg ttatgataca
     7741 gagatatccg aagaaaataa cttattcagt acaggtcaaa agcagttgat cagtattgct
     7801 cgaacgatca tcacaaatcc atctttattg attttggatg aagcaacaag taatgtagac
     7861 acggtaacag aagcaaggat ccaaaaagca atggatgaag caatcaaagg aagaaccagc
     7921 tttgtgattg ctcaccggct gaaaacaatc ttgaatgcag atagaatcgt tgtcttgcgt
     7981 gatggggaag tcattgaaga aggcagtcat catgagttat tagcagaaaa tggattttat
     8041 gcggaacttt atcataatca atttgtattt gaaagttaaa ttaaaagaac ctcgttataa
     8101 cggggttctt tttatatatt ttatgttgtt tgtttttata aggggca
//
LOCUS       DAXWOO010000127         8074 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.129, whole genome shotgun sequence.
ACCESSION   DAXWOO010000127 DAXWOO010000000
VERSION     DAXWOO010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.129"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            92..372
                     /locus_tag="ACGE70_002182"
                     /pseudo
     CDS             92..372
                     /locus_tag="ACGE70_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(450..983)
                     /locus_tag="ACGE70_002183"
     CDS             complement(450..983)
                     /locus_tag="ACGE70_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFF8693800.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(1077..2255)
                     /gene="mutY"
                     /locus_tag="ACGE70_002184"
     CDS             complement(1077..2255)
                     /gene="mutY"
                     /locus_tag="ACGE70_002184"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFF8693801.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2248..3045)
                     /gene="recX"
                     /locus_tag="ACGE70_002185"
     CDS             complement(2248..3045)
                     /gene="recX"
                     /locus_tag="ACGE70_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFF8693802.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNRGKSQKELLRKIQQTLMTKGFSQEVIQQAMADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            3152..4534
                     /gene="rlmD"
                     /locus_tag="ACGE70_002186"
     CDS             3152..4534
                     /gene="rlmD"
                     /locus_tag="ACGE70_002186"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFF8693803.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMICEINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4708..5286)
                     /locus_tag="ACGE70_002187"
     CDS             complement(4708..5286)
                     /locus_tag="ACGE70_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFF8693804.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5608..6078
                     /locus_tag="ACGE70_002188"
     CDS             5608..6078
                     /locus_tag="ACGE70_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFF8693805.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGEYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(6186..7412)
                     /locus_tag="ACGE70_002189"
     CDS             complement(6186..7412)
                     /locus_tag="ACGE70_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFF8693806.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
BASE COUNT         2358 a         1609 c         1363 g         2744 t
ORIGIN      
        1 tcttttattc tttttttatt tttgaaaact tgccatactt tttatttgtt ttattttcca
       61 gtatactata cgaaaggaag gtggctagac catgaaagtt gtacaaacaa aagatacaat
      121 gagcaatatt tacttagatg ctgtacggat caggcatcaa gtttttgtag tcgaacaggg
      181 tatgcctttg tctcgagaaa tagacaaaga tgaagcccat tgtatccact tcgtcctgta
      241 ctcagataaa aaagaaccgc aaggaactgt acgtcttttg cctttagaaa acggaaaaat
      301 gaaattacag cggatggcga tccttttgaa gatgctggca tgcctcatat ttatatgaaa
      361 aagactttat aaaaatttat ttcgaaaaaa gcaaaaaaca gcaagctttt gagaaaaatc
      421 tctcactggc ttactgttct tttttgtgtt tacttacgcg acaattgctt gtaacggtta
      481 taccaaatat caatatagcc ttccgagaac gggccatctc cattattgat ccagtcaacc
      541 agaatcttca cattttcttt taggatgaag tcaatatcgt tcggataatt catcattttg
      601 ctgtgcatct catattcatc gacatccaat agtcgtttct cgccgtcagg aaataccttg
      661 atatccaagt catagtcgat atatttcaag gcctcatcgt ccagcaaaaa aggtgaagct
      721 agattacaat aataggaaac ccccttttct ctgatcattg ctattacatt gaaccagtat
      781 tttttatgaa aataaacaat cgctggttcg cgcgttaccc atcggcgtcc atcagattca
      841 gtgactaatg tgtgatcatt cacgccgatc atagagtgtt cgcttgtttt taataccatg
      901 gtatctcgcc acgttcgatg caatttgccg tcgtgcttgt aactttggat cgtcacaaac
      961 tctccttctt ttggaactcc catagcaacc cttccttaag cagcattttg tgctttgtgt
     1021 ttgggtgttc attacaccga catcataatt attatatcaa taaaaatgag ctttgcctag
     1081 aaaggattcc tattgtttcg atttttctct aactgatcta ccagtttcat ttggacctta
     1141 ggaaaaacga ccgaatcaaa tgcatcaggc ttcaaccact tcgaagtttt gttctcggta
     1201 aattcttgtt ctgctccctc ggttgcccgt ccgtaaaaaa ggagaacatg ccatttcaga
     1261 tgactaaaaa tatgagtaac ttctcctaaa tgtcgtgtct gccaaacaaa gagttcctgc
     1321 ttttcaaatg ggagattttg ctcttcttct gctactaggt catcaaacag atcgatttct
     1381 ggctttgttt cccattcttt tttcaatcgc tcatactcct cttgtgtcac ttccatcatt
     1441 ggaaatgtcc acatattcgc cagcaatttt tggctatctc tttcttcaaa ataatacgct
     1501 ccagaatgat tttgaagagc cgcactgata taataaacgt cttttggctt tgcttttttt
     1561 gtttttacag gaaaactcgt ttggattcct cgtttgttag ctaaacaaaa ggcttggatc
     1621 ggacaagtct cacactttgg agaagttggt gtacagatag ctgaacctag atccatcatt
     1681 gcttggttga attcacctgg atgcttttca tcaatgattt tccgcatcgc ttcatcaaag
     1741 attttcctgc tagaagcttt tgcgatgtct gcttcaatgc aaaacaatct gctgactact
     1801 cgcatcacgt tgccgtcaac cgcgggttct ggaagtccaa atgcgatact tgcaatcgcg
     1861 cctgtcgtat atggtcctat tcccttcaat gagctaattt cttcaggcgt ttgcggcatt
     1921 tccccatcaa attctgacat gatctgttta gcagcagctt ggatgttccg ggctcttgag
     1981 taataaccaa ggccctccca tgcttttaaa agcttttctt ctggagcatt tgctagctct
     2041 tcaattgtag gaaaccattc catgaaacga taaaaataat cgatcactgt gtctactctg
     2101 gtttgctgaa gcatgatctc agagatccag atacgataag gatcacgatt atagcgccaa
     2161 ggcaggtttc ttttttcttg ttcataccat tgaatgaact gatcttgaaa ttcttttgtt
     2221 tctttatctg tccatttttc ccattcttta ttcactttcc tcttcctttt ccgtaatgaa
     2281 tcgctggatc atatccaaat caaatccttt ttgatataag ctttgcttta cttttagatt
     2341 tcttttttta ggttcaaacc ttaaatttct tcgccatagt ttatctcctt gtttgactaa
     2401 ataatcatat tctatttctt gatctgcttc ttgcggaaga tcagccatcg cttgttggat
     2461 cacttcttga gaaaaacctt tcgtcatcaa tgtttgttgg atttttctca ataattcttt
     2521 ttggcttttt ccgcgatttt ttccaaacgc tttttcggct gtctgatgtg cattttgaac
     2581 ttggtcttca aatgtatatt ggctgagcgc ttgttcaatg atttcaggtt tcaaaccttt
     2641 ttgctgcatc tgctgcgcta atttctttgg tcctttgtca cttaagcgca tattcgtgcg
     2701 cacataactt tccccataga ccaaatcatc gacaaggtcc aattctttta agcgggctac
     2761 gattttattt cgatcttctt gaaggatttc cttttctttt aagtatgttc ggatttcttt
     2821 ttccgatcgc aactgataac ttaagtaatt cattgctagg tgaaaaccga aatcagctcc
     2881 gctactcttc ttgatctctt ggatagtttc ttcgtctaat tcccgtcctt ttaataatcg
     2941 atagcgtact aatacatctt cagatacttg taaagcttct ccttcagata aatctacgcg
     3001 atagaaaggg cctttgcctt tactgattct cgtaacggtt aacataaaca tcctcctcga
     3061 acatatattc tctatcatac cacagaaaat ccccgtaatc aggtattata tgttagaata
     3121 aaccacagaa aaaagaaaaa gggagatact tatggcagaa acaattgtca aaactggaca
     3181 aacggttcct ttgaaaatca agcgtttagg cataaatggt gaaggaatcg gttatttcaa
     3241 acggttgatc atatttgttc catatgcact accaaaagaa gaagtactag tgaaaatcac
     3301 gaaagcaact cctcgctatg cagaaggaga attaataaaa gtcaaaaaaa caagcaaaga
     3361 tcgcgtagtg gcaccatgtc ctgtttacta tgaatgcggt ggctgtcaat tacaacactt
     3421 agcttatcaa gctcaattgg attttaaaaa ggatctgctt ttgcaagcat tggaaaaatt
     3481 caaaccagca ggctttcgat cttatcaact tttgccgacg atcgggatgg atgagccttg
     3541 gcattataga aacaaagcac aatttcagtt acgaaaaaat aagcagactc aaaaaattga
     3601 agctggttta tacgaagcaa actctcatca gttagtccct ataacagact gcttggttca
     3661 agaacctcga acacaaaaag taatgaatac tgttgttcaa ctattgaata aatttgatgc
     3721 acccatctat gatgaacgga caaacagtgg aatcttccgc acaatcatgg ttagaatcgg
     3781 tgttaaaact ggggagttac aagttgtttt tattactcgc agtaaaaaat tcccgcaaaa
     3841 aaacgcaatg atctgtgaga tcaatgaacg actgcccgaa gttgtttcaa tcatgcaaaa
     3901 tgtccaggca caaaaaacgt ctctcgtgat gggggatgaa acgatccact tatggggaaa
     3961 agaagccatt gaagaacaaa tcaatgaagt gactttcgat ctttctcctc gtgccttctt
     4021 tcaactcaat cctcagcaga ccgaattgct ttatggcgaa ggaatcaaag cattagatgt
     4081 ccagcctaat gaaacgatcg tggatgctta ttgcggagtt gggacgatcg gcttaagtgt
     4141 agcaaaacaa gccaaagaag tacggggaat ggataccgtt cctcaagcaa tcgctgatgc
     4201 aaaaaaaaat gcggaaagat taggatatca caacactcga tatgaagtcg ggacagcaga
     4261 gcaagttctg cctaaatggt tgaatgaagg ttttcagcct gatggtatca tcgtagatcc
     4321 accacgtacg ggtttggata acgcattgat ccaatcatta ttgaaatacc ctccaaaaaa
     4381 acttgtttat atctcctgta atgtttctac acttgccaga gatctggtta atttaagtaa
     4441 aaagttcaag attgaatatt tacagtctgt cgacatgttt cctcagactg cacgttgtga
     4501 agttgtcgtg aagatgacga gactcgctga ctaaatgaaa gaggttgtga aaaagtctgc
     4561 cctgtatcaa gtaataagaa cagccaaaca aaaatagctc ttcatgtttt atgaataccg
     4621 tacttgtgac cgaagagtta acaggaggac actatttatt taaaaaaagg gttgtgacat
     4681 agtttttgtc acagcccttt ttgattatta ttggatattc acgtctatca cttgataaaa
     4741 tgcatttgtc gtatcgctga tattccatac tgctaaaatc acattatatc cttttcgatc
     4801 ctgcggaagt gtgatcgttt ggttgactgt ttttggtgga acagttgctt tgtcgtctat
     4861 ttttgtgatc aattcaaaat cactaaattt caaaggttga ttaggattcc aagttggttt
     4921 ggtaatgtag taatcccaag tacttgtccg atgttgtgct gttaaattcc aagtaatatt
     4981 taatgttcct ggattaacca tgtttttatg ccatctgtta gatgtctgtt catctaaagg
     5041 ctcaaagccg ctgactcctg cactagcaat ttttccatca atgaatgtat ttttggtagc
     5101 ttcgatactt tgcggttccc attgtgctct tccgacattt tgatttaagt ttcctgctgc
     5161 tgatgttcct aggtatgcac ggcttcctgg agaagtaata taaccatgcg caaaacctgg
     5221 aatgctaaaa ccgaacaacc ctaaactaac taacccaata aacaataacg tactaatctt
     5281 tttcatgtag aaacctcctt catttgataa ccacattgta aacgctttat tgtattaaca
     5341 caaacattat gcttttataa ttttatgttg aagtaaaaaa ggtgaaaaaa gtaagatgca
     5401 gattataaca gaagtagacg agaaaagtac aaatagaaaa catgagaacc aaatcatatg
     5461 atctttttca aaaacatcat tcatataaac aaatgttcaa ctttttaata acaacaatca
     5521 tcaatttttt tcttacttca caaactaaat acaggtcttg ctgtgagcct tcatggtaaa
     5581 ctactattat agaaaggagt tagtaaaatg aagatgataa aagatattcg tttttgggtt
     5641 tgtgttatag gaattgtcat attaggcttt ttatcagggt tgcttagcgg aaatcccgga
     5701 gaatattact attctttgca acttccacca tttgcaccac ctagctggat atttgggccg
     5761 atgtggacct tactgtatat attgatgggt atttctcttt atttgttact taatcacaac
     5821 aacaaaaaac agcgaaacaa tcttgtcggt ctttttgtga ttcagttcat ttttaatttt
     5881 atatggtctg ctctattttt taatttaaga aatattttca ttgcagcaat cgatattaca
     5941 cttttagtta ttttcttgtc agtgcttatg tatcagttgt ggctacatca tcgcctggca
     6001 atgtggctca tgattcctta ttatttatgg gtactattcg caactctatt gaattattca
     6061 atttattttt taaactaagc gctaaaaaga gacgtcctgt aatttaacac atagaaaaag
     6121 tgccacctaa gttagatttt ttgtctaatt taggtggcac tctttattaa ccaaactatt
     6181 tttttctagt acccacttgt tgttccatac tcattattct ggtcataacc ataaccattt
     6241 tgttcctgtc cagcgtaagg atcataactt tgagtatctt gacttggtgt atagtactga
     6301 cttgtatctt ctgtttgctg ataataggta ttcggatctt ggtactgatc atatgtgttt
     6361 tctgaatagg aagaataatc ggtgtaccct tcactattcg atccatcacc gtttgtcgaa
     6421 tcgccatctt ccgaatcatc aaagaacatc attgaagcat tctcattttt caaattaggt
     6481 aatgtatcgc ttgtttttat atttaattgt ttcttcagtt cattttggat cgaaagaaga
     6541 tcattttttc ctaaaatttg atagtagata tcattgatca tttgtccttc gccttgcaac
     6601 tggtcttgtt tgattgtttc aaacgcaggg gtgtagttcg tcgctacatc aagcatgtca
     6661 tcccaatcta agtccgtcgt cacattgttc tcaactgctt ttaaaatttt tctataattg
     6721 cttacgccat ctaaacttaa aactttattg acgattttcg tcactacttc ccgctgacga
     6781 cgttgtcgac cgatatcgcc ttctggatct tcgtgtcgca ttcgagaata ggctaaacct
     6841 ttttctccat tcaatgtttg ttcacctact ggtacatgga caccatccaa agtaaagtca
     6901 attttattat tcactttgat gccaccgacc gcattgatca aatcttccat gccatccaaa
     6961 ttgatcgtta cataatgatc aataggaata tcgagcagtt gctcaattga atccatggcc
     7021 atttcgacac caccaaatgc ataggcatga tttaatttat ctaccgttcc ataacctacg
     7081 atattcgtat aaatatctcg gtccaaactg atgatcgttg attttttttg tttcggatta
     7141 actgttacta ccatcatcgt atcagaacgt ccttgttccg ttcttcctaa tccaccggta
     7201 tctaaaccta atagcaaaac cgaaaaaggt tctttgtcat caatgcttac tctctcagaa
     7261 cgtttggagg atattcgatt agagttttta gaaattttgt ttactgtttg gctcgcttct
     7321 cccataaatc tgattccgta tgctgataat ccggcaatca gcaaaacaaa tacccctaac
     7381 aaggttaata gaactttttg ccatcgcttc ataatacacc tctgtttcac attttctaaa
     7441 atattgtctc atataactgt tcaatcagca aattcacttt ttaatcataa gaaaacttta
     7501 aaaaacattt ttgtgtaacg attactattt tttaaaattt ctcaaaattc ttaactatca
     7561 taccataccg tgaaaaagat cgaaagaccg aatacacgca gttcgcaatc gttttatctt
     7621 tttttacgta ttttttccag aaaaagcgaa caacaaggta ttttccaaat aatcgtgcag
     7681 taaaatgatc gcacatacct ttcccatctt tttaacagtc tttgcctacc gttcagatta
     7741 tcattcgatt tcacgaaaat ttcttcctat gactggtcct tttgttaaac ccatcttcta
     7801 ttcgttttat ttccagctca ctgcatggct cttaaaatga ctcgaactag tgatttcatt
     7861 accatcatct tttaatggat agatatgctc ctcttcaaag taaaacgatt ttcttttaaa
     7921 gtaatagaat tgacatggta atgagtatgt atactagtgg tttcacaaat ggtcttttat
     7981 tcaacatcca aaaactgaaa tttctttaaa atccattact aacgaagaaa atgttccttg
     8041 cttgataatt agttaggaca tgtcaagtga acaa
//
LOCUS       DAXWOO010000128         8060 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.130, whole genome shotgun sequence.
ACCESSION   DAXWOO010000128 DAXWOO010000000
VERSION     DAXWOO010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8060
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.130"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            126..1217
                     /gene="phnW"
                     /locus_tag="ACGE70_002190"
                     /pseudo
     CDS             126..1217
                     /gene="phnW"
                     /locus_tag="ACGE70_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
     gene            1210..1995
                     /gene="phnX"
                     /locus_tag="ACGE70_002191"
     CDS             1210..1995
                     /gene="phnX"
                     /locus_tag="ACGE70_002191"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050194 - phosphonoacetaldehyde hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFF8693807.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(2341..4032)
                     /gene="argS"
                     /locus_tag="ACGE70_002192"
     CDS             complement(2341..4032)
                     /gene="argS"
                     /locus_tag="ACGE70_002192"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFF8693808.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(4454..5401)
                     /gene="arcC"
                     /locus_tag="ACGE70_002193"
     CDS             complement(4454..5401)
                     /gene="arcC"
                     /locus_tag="ACGE70_002193"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFF8693809.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(5516..6535)
                     /gene="argF"
                     /locus_tag="ACGE70_002194"
     CDS             complement(5516..6535)
                     /gene="argF"
                     /locus_tag="ACGE70_002194"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFF8693810.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(6626..7855)
                     /gene="arcA"
                     /locus_tag="ACGE70_002195"
     CDS             complement(6626..7855)
                     /gene="arcA"
                     /locus_tag="ACGE70_002195"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFF8693811.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         2306 a         1640 c         1464 g         2650 t
ORIGIN      
        1 atcatttttt attcaatttt ttcaacacct tgattgaatc acaataatca tcatttatat
       61 tggatgttaa caagagcagc ttcttgctct ttgtgtattt caaaaaagaa atgaaaggaa
      121 atcatatgta taaattatta actcctggac cattgacgac ttctgaaaca gtgaaaaatg
      181 ctatgctgat cgatcattgt acttgggatc aagaatacaa agaaattaca caatggataa
      241 gaaaaaaatt attggatctt gctcaagtat cggaaggaat atacacaacg gtcttaatgc
      301 aaggtagcgg ctcctttggg gtagaatcgg tattaagttc tgtgatcagg ccagaagata
      361 ctgtactgat ttgttcaaac ggcgcatatg gacaacgaat gatcaacatg tgcgaatgtc
      421 tgaaactgaa ctataccgtt tatgaggtat cgtaacatca aatcctccaa gctcatgtga
      481 ttacacagct gattgaagag aatccctcta tctccgcact agcaatggtc catagcgaga
      541 ctacttccgg tattttgaat aatttggaag agattgctgc tgtcacacaa gaaaaagatc
      601 ttttgtttat agtggacgcg atgtcaagct ttggtgggat accgattcgt gtagccgaac
      661 tagggattga ttttttgatt tccagctcaa acaaatgtat tcaaggtgtt ccaggctttt
      721 cttttgtact tgctaataaa gcggaactag aaaagcgaaa aaatgtgagt cgaagtttgt
      781 cacttgatct atatgaccag taccgcgtga tggaaaacga gaatggcaaa tggcgtttca
      841 cttcccctac ccatacggtt ttggcttttc aacaagcgct gaaggaacta gaggcagaag
      901 gaggaatcga aactcgatat tcacgttatc tcgccaataa tcatgtacta cgacaaaaaa
      961 tgacagagat ggggttccag cctttcattc aagaatatca ggggccattc atcacctctt
     1021 ttctttaccc taaggactgg cgatttgatt ttaatcattt ttatcagtat ttaaagcaaa
     1081 acggatttgt gatctatccc gggaaaatca gcgatcatga atgttttcgt atcggaaata
     1141 ttggcgagat ctatccggaa gatattttgg aactgacagc aattatcaaa gactataagg
     1201 agaacgttaa tgattaaaac tgtaattttt gattgggcag gaacaactgt ggactttggc
     1261 tgtatggctc ctgtccacgc ttttagaaat gcttttctag aaaaaggaat ccaactgact
     1321 gacaaagaaa tccgcgaacc gatgggaaaa ttaaaatggg atcatatcca acagcttctt
     1381 tccctccctt ctgtcaaaaa acaatggata gctatttatg gacaactacc tcaggaaaaa
     1441 gaggtggagg aattgtatca acaatttgaa cattatctgt ttaaagattt ggtcaagcat
     1501 tctaccttga aacccgacac attggaaacg atcgaacggt tgagaaacgc tgggatcaat
     1561 attggttcca cgactggatt tactgctcct atgatgacaa tcgtagcaga aaccgcaaaa
     1621 aaagaagggt attcaccaga cttcatggca acttctgaag acgtcgaagg gtatggtcga
     1681 ccgtttcctt atatgatcca taaaaacatg cagtacttca aaaacaagtc ggttgatgaa
     1741 gtaatcaaag tcggagatac ggtctcagat atccaggaag ggaaaaatgc cggagtgttg
     1801 acagtaggtg taattgaagg tagttcgcta atggggattt cttatgatga gtaccaaaaa
     1861 ttagagatgg ggaaaaaaga tcgtttaaaa cagtctgttg ctcttagatt ccaacaagct
     1921 ggcgctgata tttgtgtaaa caatttaaaa gagttaacag aatacatttt gtacgctgac
     1981 aagaatccag aatgactttt atacaaaatc agaggtcgtg aaaaagctgt actgcatcaa
     2041 gtaataagaa cagccaaaca aaaatagctc ctcatatttt tcgtttggtt aggcttatta
     2101 ccgcagatgc aagcttttga acaccgttta ttcgaaaaaa aaagggagct gtgacacgac
     2161 ttttgtcaca gtccccttat ttacaaacaa acagtgttta agagacaacc tctccactaa
     2221 acggtgttca ttcagctgac tctcggtatt tgctcaaaaa acgataaaat ttgaaaagct
     2281 attttctctt ttgtttctct aatactcgga tcagaacgct gatttcacat tctctttttc
     2341 ttacatctta tctggtgcat gaagtcctag caaacgaagg tcttctttca ataatacagt
     2401 cactgcataa actagagcta agcgtgcttc tttttgttca tcatcagcta aaatcttcgt
     2461 atgagcatag tatttgttga atgcttgtgc taatttgatt gcatgtttcg caatcactga
     2521 cggttcgtat ttttccccag cagacaagac cgtttcaggg tatttttgaa ctaatttaac
     2581 cacttcccag ctggtatcgt cattcaatgc atagtctgct gtttctgaag ggacaaatcc
     2641 tgctttttcc aacaggctga ctgcacgagc atgtgtatat tgcacataag gtccagtttc
     2701 tccttcaaat cgaacaactt cttccaggtt aaagtcaaat gtattcagtc gatcgttttt
     2761 caaatcgtgg aaaatgactg ccccaacccc aacttgttta gctacagcat ctttgttttc
     2821 caaatcaggg tttttctcgc tgatttgttc tttagcagat tggattgctt catttaatac
     2881 ttcttctaat aagacgatct ttcctttacg agttgataat tttttaccac cttgcgtgat
     2941 caaaccaaat ggaatatggc gcatatcgtc agaccagtca aagcccattt ccttcaagac
     3001 agctttcaac tgtttaaaat gatagctttg ttcattccct actacatata gagattgttt
     3061 gaagtcgtaa gtacgtttac ggtacaatgc agcagctaag tcacgtgtaa tgtatagtgt
     3121 cgcaccgtct gacttcttaa tcaacgctgg attcaggtca taagcagata ggtcaacgat
     3181 ctctgcacct ttatcctcgt tcagcaaatg tttttcttct aaaagtttga cgatttcatc
     3241 catcttgtca ttgtagaaag cttcaccgtt caacgaatcg aaacgtactt ccagcagatc
     3301 atagatttta ttgaattctt tcatcgattc atcacggaac cattgccata attgaatagc
     3361 ctcttcatcg ccttcttcca gacgtttgaa ccaagcacga gcttcttcat ttaattctgg
     3421 ttctgtttca gctacttcat ggaattggac atataaacga agcaattcat tgattggttc
     3481 tgctttaaca gcttcttctg taccccattt tttgtaagcg acgatcagtt ttccaaactg
     3541 tgttccccag tcacctaaat ggttgatgcg gattggctga taaccgattt tttccataat
     3601 gaaaccgatc gaattaccga taactgtcga acgcaaatgt cccattgaga ttggtttggc
     3661 aatattagga gaagacatgt cgattggtac tgttccttga ttcccgatat tgctatcccc
     3721 ataatgttct ttttctttca ctactgtttg caaaactttt ttgctgataa gttctttatt
     3781 catgaaaaag ttcaagtaag gtccgacaac ttcgattttt tcaaagttgg cagaatcgat
     3841 tttttctgct aaatctgcag cgatttgctg tggcgctttt cggtaaactt ttgctaatga
     3901 aaaggctgga aatgcaacat ctccgtgttc agctgatttt ggattttcta gtaattgttc
     3961 gacttgttcc agtgttaaat catctttgac agcgtcatag acagcttttg cgacaagatc
     4021 tttattattc atctttattc ctcctaaagt gctcccttga ttgttaaaca aaaaaaatct
     4081 ctagcgacgg catacttagt atgccatcac tagagacgag atttcccgcg gtaccactcc
     4141 aattgtccta aaaaaggacc agctccatct gatatcggta gacgaccgtc tgtttaaaac
     4201 agaattctcc aagtgcgctt cgttgtatct tcttcattcg gcttccacca cttccgaatt
     4261 cgctagccaa gaatgataca agtactctct tgttcaacaa atcttagtct tattaaactt
     4321 tcttgaatta tatcagaaaa aaaaacgaaa ggctagtcgt ttctgataaa aaaacaatcc
     4381 agatggccta aaggagaaga aataccacct ggatagctat aaatcatttc gtaaatacga
     4441 aagacttaac aaattaatca gctacgataa tcgttccttc ttcagaagct aaaagattct
     4501 cgatattttc taaagaagtg atgattgcct tagcgtttgg ttttgcttca acaaatgcaa
     4561 tagctgcctc tactttcgga agcatgcttc ctggagcaaa ttgcttttca tcaatgtatt
     4621 gtttcatttc tgaaacagta acagtttcta atttcttttg atctggtttt tggtaattga
     4681 catacacatt gtctactcct gtcaatacga tcagcaaatc cgcatcgatg atttcggcta
     4741 atttttctga agcaaaatct ttatcgatta cggcttctcg tccttctaat ccagtagctg
     4801 tttctacaac aggaatccca ccgccaccaa cagagactgt gataactcct tgatcgacca
     4861 gcgtttcgat gacacgtgct tctttaatcg agatgggttt aggggaagcg actacttttc
     4921 tccatccacg tccagcatct tctttgaatg tcacagtgga gtcagcattc atttgttctt
     4981 tggcttcttc ttccgtgtag aatggaccga ctggtttact tggatttttg aatgaaggat
     5041 cattttcatc cacaataact tgagtgacta aagaaaccac atctttatcg atcccttttt
     5101 ccttcagtac ttcgcccatg gcattttgta accagtaacc gatagatcct tctgtcattg
     5161 caacacatgt atctaaaggc attgccggtg ttttctcaga atcagcagct tgctgctgaa
     5221 tcaataggtt accgacttgc ggaccatttc cgtgggagat gatcaattca tctccttgtt
     5281 cgataaattt cactaaatac tttgctgttt ccattaacgc ttcttgttgg gcttttgcac
     5341 ttgcatccgt agacaagatt gcattacccc ctaaagcgac aactactttt cgtttggcca
     5401 tttcattcgt cctttcttca gttctaccga attttctaga taacaaaacg agccagcgtt
     5461 ttgtgatttt ccctcaatgc tgactcgttt tttcgtgaga tgaatgtggt tatgcttaaa
     5521 cgcgagggat aaacaagtta cctaatgttg ctgccatgat cgctttgatt gagtgcatac
     5581 gattttctgc ttggtcaaat tgacgagcat atttgctgcg gaagacttca tctgttactt
     5641 ccatttcagt gatgccaaag cgttctttca tttgttcacc gtaaacagtg tttgtatcat
     5701 ggaaagctgg caagcagtgc aagaagatca aacgatctgt gttgtgtgtt ttttctacca
     5761 tttccatatt gatttggtaa ggtttcaaca atttgatacg ttcttcgaat ttatcttctt
     5821 cacccattga tacccataca tcagaataaa ggacgtctgc tccgtctaca cctttatcaa
     5881 catcgtcggt aatcatcaat tgtgcaccgg atttttcagc aaagccttct gccattttga
     5941 cgatttcttc ttcaggttgt aattcttttg gtgcaacgat acgcatattc gctcctaaaa
     6001 ttgcacctgt gactaataaa gagttagcca tattgtttcg accatctccg cagtaagcaa
     6061 cagtgatacc ttctactgtt ccaaagtttt cttggatagt taaaaagtca gcgatcattt
     6121 gtgttggatg ccattcatca gtcaagccat tccaaactgg aacaccagag aattctgcta
     6181 gttcctctac cattttttga ctgaatccac ggaattcgat tccatcaaac atacgaccta
     6241 gtactttagc tgtatcttct gtggattctt ttttacctaa ttggatatca tttgcaccta
     6301 gatattcagg atgtgcacct agatcgatcg ctgcagtagt aaatgcagaa cgagtacgtg
     6361 tagatgtttt ttcaaataaa agcgcaatat tttttccttc tagatagtga tgtgggatac
     6421 cacgtttttt caagtctttc aagtgttctg aaaaatcgat caaatattgt aattcttctt
     6481 ttgtaaagtc tttttctgct aataagcttc ttccttggaa tactgattct ttcatattaa
     6541 acattctcct ttagattgtt tttatttaag attaaagagg aaatcccgac ggcagctgac
     6601 agtaagaccg ccgtcaggaa acaaattact tcaagtcttc acggacaagt ggttggctca
     6661 tgcagcgtgg accaccacgg cctcgtgaca attcacttga attgatttct aacactttga
     6721 tgccatagct acgaagcaat tcgttcgata cataatttcg gttataagtg actacaacac
     6781 ctggagcgat tgccaatgtg tttgatccat cattccattg ttctcgaggt gcaacgatct
     6841 catctccatt accagttggg atcagtacaa gatcatctaa tccaagagca gctttcatcg
     6901 tttcgtgtaa gttatctgaa tgagtaatct tgatatcatc accatctggt tcgattgtaa
     6961 agacatccat cttaccgtct ttactttgaa ttgctggatg gatagtgaat ttatcgtgat
     7021 ctaccattgt aaatacggta tccaaatgca tcattgcacg gttgtttgga attttgattg
     7081 ctaacacttt ttcaaatcct gaattttttg cgaataggtg gcgtgctaat ttttctaaag
     7141 ctttcgcatt agttcgttga gaaatcccaa cagctactac tttatcgctt aagatcaatt
     7201 cgtctccgcc ttcgatatga tctggatttt cacgatccaa ccatacttcg attcctttat
     7261 ttgcaaatct tggatgatgt ttcaaaatat attctgtaaa catcgattca cgacgacgtg
     7321 cttcaaaagt catcttattg actgtcatcc catttcccat tgatgcggaa gggtcgcgtg
     7381 taaagtacaa gtttggcatt gggtccatgt aaaatggata gtcatcgtct gctgacagat
     7441 cgtatagact gctagaacgt acttcaatat cttttgtacg aaccccagcc atgattttat
     7501 ctaccatttc ttgcgtttcc attgatagaa ggaactcatg aagaccctct cttacagcat
     7561 tcgatgcaat atgagattca tccaacattt tattcaagaa ttgttctttg acattgcctg
     7621 cgtcaatcgc ttctgccgct aatttttcca agtaaagagt ctcgacgcct tcattttgca
     7681 atgtctttgc aaagttgtca tgttcttctt gagcaatcgg aagatatgga atatcatcga
     7741 acaacagacg atccataata tctggtgtca aattttccac ttcttgtcca ggccttttca
     7801 gtaaaactgt ctttaatttc cctatttcag agaaaacgtg aataggttta tccatgttgt
     7861 gttgcctcct tctttttgat tacatgtata ggatatcctc cttttgtaat cactttcata
     7921 attttgtctc attcaaaatg agacattcct cacattaata ttcgataatt aaataattaa
     7981 acgtttttct atttttaagc gtaatttatt cattttttat tctgtataca ctttcaaaaa
     8041 aatatttatt cattgaaaaa
//
LOCUS       DAXWOO010000129         8003 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.131, whole genome shotgun sequence.
ACCESSION   DAXWOO010000129 DAXWOO010000000
VERSION     DAXWOO010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.131"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(220..600)
                     /gene="yajC"
                     /locus_tag="ACGE70_002196"
     CDS             complement(220..600)
                     /gene="yajC"
                     /locus_tag="ACGE70_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFF8693812.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(697..1842)
                     /gene="tgt"
                     /locus_tag="ACGE70_002197"
     CDS             complement(697..1842)
                     /gene="tgt"
                     /locus_tag="ACGE70_002197"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFF8693813.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            2060..2758
                     /locus_tag="ACGE70_002198"
     CDS             2060..2758
                     /locus_tag="ACGE70_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFF8693814.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2926..5565)
                     /locus_tag="ACGE70_002199"
     CDS             complement(2926..5565)
                     /locus_tag="ACGE70_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFF8693815.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(6184..7215)
                     /gene="queA"
                     /locus_tag="ACGE70_002200"
     CDS             complement(6184..7215)
                     /gene="queA"
                     /locus_tag="ACGE70_002200"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFF8693816.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            7408..>8003
                     /locus_tag="ACGE70_002201"
     CDS             7408..>8003
                     /locus_tag="ACGE70_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8693817.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEA"
BASE COUNT         2268 a         1721 c         1466 g         2548 t
ORIGIN      
        1 tttgtgattt ttttcacagt tatttttaaa tataaaattg ctttatttac aggtgtttgt
       61 atagaaaaat atataaagtt atttattatt atttatatga cacacccgat tattcaatat
      121 tgagcaatat atcccatgct cttatctctt tcttttttga ataaaaaatg aggtaggaca
      181 tctttctgcc ctacctcgtc tttaaataag ctttgatact tatttttctt ccttctcttc
      241 tgtctcttcc agcgtttctg ttgtttcagt ttttacttca actgtctcag ctggttcttc
      301 tgttgtttca acagtagtcg ttgcatcatt tgttacgaca gtccctggtt tgaccgtttt
      361 gattgctgca cgatcgtact caaggaagat cccttcgcag tctaaaacaa ctgtccgttt
      421 gtcattgtcg atttctgaaa caactccatg taatccgccg atcgttacga ctgaatcacc
      481 tggtttcatg ctatctaaca agttttgacg ttcttgctgt tgtttctttt gcgaacgtga
      541 cataaaaaac cacattgcta atagcgcgat aaacatcaca agcattggta atgttcccat
      601 ttttttcacc tcactaattc tgtatctaca aaaaacactt tagcagaaaa atgctgataa
      661 cactagtttt ttacttaatt tttaaaagat tttcctttag aagctttttg cgttttcttt
      721 attgaacccg tattcttcaa aaaaggcttc tctaaattca agcaaattat catccatgat
      781 tgcttgacgt acttgtttca ttagattcaa caagaagtat aaattgtggt aagaagtcaa
      841 acgaatccca aatgtttcat cacatttgat caaatggcgg atataggcac gtgtatagtt
      901 tttacatgta taacaatcac atttctcatc aattggacgg aaatcatggg catatttcgc
      961 atttttcact actagacggc cttgagaagt catacatgtg ccatttcgag cgattcgagt
     1021 aggcaagaca cagtcaaaca tgtctactcc acggataaca ccatcgatca atgaatcagc
     1081 agcaccgaca cccatcaagt aacgtggttt gttttcgggg atcaacggtg tagtgaattc
     1141 taatacacga ttcatttctg atttaggttc cccgacagat aacccgccga ttgagtaacc
     1201 agggaaatcc atcccgacta gatctttcgc gctttgacga cgaagatctt caaaccctgc
     1261 tccttggatg attccaaata atccttgacg gtctggatta gcatgagctt tcaatcctcg
     1321 ttctgcccat cgagacgtac gttctactga ttttttcacg tagtcgtaac tttcatcgaa
     1381 tggtggacat tcgtcaaaac tcatcatgat atcagagccc aatttatttt ggatgttgat
     1441 tgctttttct ggtgataaaa acatacgcga accgttcaag tgatttttga aatgcacgcc
     1501 ttcttcttcg atattccgca tatcacttaa tgaaaaaact tgaaagccgc ctgaatctgt
     1561 taaaatcggt tgatcccagt tcataaattt atgcagtccg ccagcttctt ctactaagtc
     1621 ttcaccaggg cgtagccata aatgatatgt attacttaaa ataacgccag cacccatttc
     1681 tttcaactct tctggggaca ttgtttttac tgtagctaat gttcctactg gcatgaacat
     1741 tggtgtgggg aaagttccgt gtggcgtgat caattcccct aaacgcgcgc cagtatgttt
     1801 ttctttcttg attaaacgat agcgaatggc aggttgcgtc attcatttcc ctactttcta
     1861 tataaaatat cttgacaact cccaccatca taatcgtttc aagtgctttt tgcaagttcc
     1921 ctcggcttcc ttctacttta tttaatattc ttttttttat ccttttttta tgaataacaa
     1981 aaaaaagaaa aacattatga cagaaatatt ttttgataca tggtatattg gtgttattaa
     2041 agaaaaggag ggaaaaatca tgcaatcttc tgcagaatta aaacgccaag caaaagagag
     2101 tctaaaagga cgttggggac aagctgtttt actaaatcta atccctacat tgattggtat
     2161 tgctactatt cttcttatta tgattcctat tatcttgttt gccaccacca tatatgatgg
     2221 atcagcgagc gtctcagaag cgacaacttc aggaggcggt agttcaggag gcggtggcgg
     2281 aattatttcc agcatcatta gcgctttgtt catgagcggt atttcttgga cttatcttga
     2341 tattttacgt ggacaaaaag atcagatcga accattcaaa gatgcatttc gtggattcaa
     2401 aggtttgttt atcggcggag tgatcagctt agctgtgctg atctcgatct ttacaacatt
     2461 atggggtttg ctattagtta ttcctggaat cattaaaagc tattcttatt ctcaaagcta
     2521 ttttatttac tacgatatcg tccaagaaac aggacaaaca cctaaagtac tggatacgat
     2581 cactgcaagt cgaaaattga tggatggtta taaaggacaa cttttctggt tagatctttc
     2641 atttatcggc tggcatatct tagcattagc aactgctggt attggttatc tatggctcag
     2701 cccatacatt tctgcaacaa aagcagcatt ttatgaacaa ttgccaaaag atatttaaaa
     2761 gagagtgtga cttgtgaccg agggggtggc aggagaacac cgtttatcga gaggcagtaa
     2821 aagaaacatt cagcccatta gcaaaaagga ggtgtgacaa cattcatgtc acacctcctt
     2881 tttgtatagt tccagtaaaa agaaaatcaa cttagaaaca gttaatcatt cctcttcttc
     2941 tgcttccggt aatcgatgga acttgatcag ttcattcacc gcgattggta cgatagataa
     3001 aatccctacc aagaaccagt gattcaaact gatgtgtact aggccgaata gttcttgcgt
     3061 aaatggcagc aaagcaacag ccgttgtaat agctaatgcc agtaacgcca tttctaccaa
     3121 agctttattg gtcgctaaat tgactttgaa gatcgattgg ctgctgcgta cattgaacac
     3181 atgcagaatc gatgaataag ctaagatcaa gaatgcaaca gtttgaccga catgctgact
     3241 tgcgtctacc catgggctga tagtatcgac ataactacct aaataataac caaacaaggt
     3301 tacgatggtg aagacccctg catttatggc aattttttgc aataaacctc gagcaaagat
     3361 cccttcattt ttcggaatgg gtggctgatc catgatgccg tgttccgctg gctctcttcc
     3421 taaagcaaat cctggaagtc catcagcaac tacattgaca aacagtagtt gcacagctgt
     3481 aaatggtgct ccccatccta acagcatcgc aatcaaaaca ataaagatct ccgaaatatt
     3541 acagcttagc aaaaagttga tagctttacg aatattctga taaaccgaac gtccttgact
     3601 gacagcgtct acgatcgtcg caaagttgtc atccgtcaaa atcatatctg cggcactttg
     3661 cgccacatct gttcctgtga tccccatggc acacccaacg ttacttgctt tcaacgctgg
     3721 tgcatcattc acaccatccc cagtcatcgc tacgactgca cccgtacgtt gccaagattt
     3781 gacgatccgg atcttgtctt ctggcgttac ccgagcatag acagagagag ctttgacacg
     3841 cgcatctaat tcttcgtctg ataattgatg cagttctgat cctgaaagtg cttctgacgg
     3901 atcattcaag atacctaatt ctttcgctat ggcactggca gtcaccacat gatctccagt
     3961 gatcatgact gttttgatgc cagccttttt cgctcgcttg attgctcctt tactttctgg
     4021 acgtggtgga tcgatcattc cgatcagacc aatcaaacgt aagtcttttt ccagtgcttc
     4081 agaagaaata tcttgcggtt cttcgtcata agtagcataa ccaacagcaa tcactctcag
     4141 tgcccgttta ccaaagcgat cattaacgat cgccgcttgt tctacatcgc ctgaactaaa
     4201 gcgcggagca agaacatcga atgctccttt ggtgattgag atgtattttt cgcccatttg
     4261 gtgaactgtt gtcatcattt ttcgttcaga atcaaaagga agctcgttta ctcgtggata
     4321 tttttcttct aattctgcct ttgtatgata attctcttca acagccctta cgatggctac
     4381 ttctgttgga ttacctttga taacaaggtc gtcatcttct tgctcgaccg tcacatctgt
     4441 acatagtgca gccattttca atatttccat cgcgctgttc gtcattgaat cttcgatatt
     4501 cgttacttca tcttctttgc tccatacccg gcggacgcgc atttcatttt gtgtcagcgt
     4561 tcctgttttg tctgagcaga taacattagc ggttcctagc gtttcaactg ctggcaactg
     4621 tcggatgatc gcatgtttcc tcgccatctt ctgcacaccg tatgcaagtg tcaacgtaac
     4681 aataacagtc aaggtttccg gtacagctgc aacagccaag gaaatcgaag tcatgaacat
     4741 ctcaaggagt ggttctcctt gcagttcacc aatcacaaat actaaagccg cagccgctaa
     4801 agcgatcata ctgattcgtt tgcctagctg attcaatcgg acctgtaaag gtgttttttg
     4861 catagtattt tcgttcaaaa gatccgcgat cttacccatt tctgtagcca tgccaatggc
     4921 agtgacgatc gctttcccac gacctgcagc aaccgtacat cctttaaata ccatattaag
     4981 ttggtcacct aatgactcat cttcttcagc aataaatgct gcatcttttt caactgcttc
     5041 actttctcca gtcaacgccg attcttctac acgtaattgt gatgcttcga tcaaacgggc
     5101 atcggcagaa atagcatcac cggactctaa aacaagaata tcacccacta ccagttgctc
     5161 tgactcaaca gtggtcactt tgccgtctcg tatgacagtt gccgtttgtt tattcatatc
     5221 ttgcaaggat tccagtgctt tttctgcgtt tccttcttgg acaatcgcca ataccgagtt
     5281 gatcacaacg atagcgatga tcagcagtcc ttcaaataaa tccccatgtt ctgtagcaaa
     5341 tgcggtataa aaggaaatca ccgctgctac aagaaggatg atcgttgtaa aatcggacaa
     5401 gcttcgaaag aattttttga tcatcgattc tttcggcgcc tcatcgaatt tattcaatcc
     5461 ctgctttttt cgccgttcat ccacttcttg tgaagacaac ccagtatctg gatctgcttc
     5521 tgttaccttc tttacttctt ctattgtctg cttgtattct tccatcctat tcctccttta
     5581 aaatcttcgt acaaaaaaag gtacttattt tactataaca tccttttttt atttacttca
     5641 aataaagggc ttatcgatta tttgttaaga aagcgtaaaa tttaaagtgc ttatatttgc
     5701 aaacgctacc aaaggatgct attatataga tgtaaggaag gaggatacca tcaaatagac
     5761 agaagaattt ctcgatagaa cagtgtaagc aaacaaagtt gttagaagta caggtttcac
     5821 ccagacatgc gtgtagagta caaacctata aatagtgaat gcgtatggat gtttggatca
     5881 cgaagtatcc gaaacaagaa gtaatgcgat acgtaacaat tgaataacgt aaacgtctga
     5941 aaattgaata ggaacaagag atgtttcaaa tgttgaagat gtaacgaaaa caaaattttt
     6001 gtttaacatg aagaaattca acaccgtgct ttgcatgtat gatggaaagt tacaatcatt
     6061 tctgtttatt tgaatccttc cgaggtacaa gaaagccgag cttatcgaga gctcggcttt
     6121 ttctttgtca taaaatcttt gtaaagcttt tttgttattc aaaactagcg gcttttcata
     6181 accttatttc acgaacatcg catctccaaa actaaagaaa cgatagcgct cttcaatcgc
     6241 atgctggtaa gcagataaag tcaattctct tcctgcaaag gcactaacta acatgactaa
     6301 agtcgatttt ggcaaatgga aattagttga gaaagcttct accactttga attcatagcc
     6361 aggcgtaatg aaaatatcag tccatccgct gtctgctttg atttctccat taaatttcgt
     6421 tccaatcgtt tctaatgttc gaattgaagt tgttccgaca gccacgattt tcccgccatt
     6481 tttacgtact tggttcaatc gctcagccgc ttcttccgtc aaacggtaga attcactatg
     6541 catatggtgt tcttcaattt tatctacgct gacaggtcgg aatgtaccta accctacatg
     6601 tagtgtcaga tagactaatt caacaccctt tgcttcgatc tcatcaagta attctttcgt
     6661 aaaatgcaat ccagctgttg gcgctgcagc agaaccattt tctttcgcat agacagtttg
     6721 atagcgatct gggtcttcca gacgttcttt gatatatgga ggcaatggca tttctcccaa
     6781 tgattcaaga atctctagaa aaattccttc atactcaaag tcaatgatcc gtccgccgtg
     6841 ttccaattct tctttcacta ctgcttgcaa tctgccatca ccaaaagaga ttcttgtccc
     6901 aactttggca cgtttcgcag gtttgatcaa tgtctcccac gtatctcctt gtgtattcgt
     6961 tagaagaagg acttctaaat gcgcaccagt atctggtttt tctccatata aacgagcagg
     7021 cagaactcgt gtattattca taactagcgc atcaccaggc tgtaactctt caatgatttc
     7081 atggaaatgt ttatcttcca tctctccagt tttccgatcc aaaacaagta aacgagaatg
     7141 atcgcggtct tttaatggtg tttgtgcgat taattcttcc ggcaagtcaa aatcaaaatc
     7201 ttctgttgtt aacatatcgt cactcacttt ctatcatgtg cttcatcatt ctatcatgtt
     7261 tttcgttctg ttggcaaaat cagcactttt tcacttctat tttttgtttt cctaaataaa
     7321 aaaactaccc tatttgtatt ttttcattgt tttaggcata ctagtaatag atagttcttc
     7381 attgaaaata gaaaaagagg tggcaccatg attgaatttc aaaatgtgtc aaaaatttat
     7441 aaaggtggaa aagtcgctgt agaggacgtc aatctttcat ttgaaaaggg tgagttcatt
     7501 tgttttatcg gtacgagcgg aagcggaaaa acgacgacga tgcggatgat caaccgaatg
     7561 actgatccaa cacaagggaa aattttgatt gatggaaaat ccatccaaga aattgatcca
     7621 gtggaattgc gacgtcagat cggctatgtt atccaaagta ttggattgat gcctcacatg
     7681 acgatccgtg aaaatatcac tcttgtccaa aaattgttga aagtcagtca agaagaacgg
     7741 aataaaacag ctgaaaaaat gattgatctt gtggaattgc cccgtgagat gttagatcgc
     7801 tacccacatg aattatctgg tggtcagcag cagcgtattg gagtcgttcg tgcccttgca
     7861 gcgaatcaag atatcatctt gatggacgag ccattcggtg cgcttgatcc aatcactcgt
     7921 gattccttgc aagaccttgt caaagattta caagagcgtt tgggaaaaac gatcgtattt
     7981 gtaacccatg acatggatga agc
//
LOCUS       DAXWOO010000130         7816 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.132, whole genome shotgun sequence.
ACCESSION   DAXWOO010000130 DAXWOO010000000
VERSION     DAXWOO010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.132"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(137..655)
                     /locus_tag="ACGE70_002202"
     CDS             complement(137..655)
                     /locus_tag="ACGE70_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFF8693818.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(657..1274)
                     /locus_tag="ACGE70_002203"
     CDS             complement(657..1274)
                     /locus_tag="ACGE70_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFF8693819.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(1271..2125)
                     /locus_tag="ACGE70_002204"
     CDS             complement(1271..2125)
                     /locus_tag="ACGE70_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8693820.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(2122..3033)
                     /locus_tag="ACGE70_002205"
     CDS             complement(2122..3033)
                     /locus_tag="ACGE70_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8693821.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLTPKWVYYSGGDGK"
     gene            complement(3074..4408)
                     /locus_tag="ACGE70_002206"
     CDS             complement(3074..4408)
                     /locus_tag="ACGE70_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFF8693822.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGISDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            4616..5446
                     /locus_tag="ACGE70_002207"
     CDS             4616..5446
                     /locus_tag="ACGE70_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFF8693823.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(5690..5939)
                     /locus_tag="ACGE70_002208"
                     /pseudo
     CDS             complement(5690..5939)
                     /locus_tag="ACGE70_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6130..6324)
                     /locus_tag="ACGE70_002209"
     CDS             complement(6130..6324)
                     /locus_tag="ACGE70_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693824.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(6829..7014)
                     /locus_tag="ACGE70_002210"
     CDS             complement(6829..7014)
                     /locus_tag="ACGE70_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693825.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
BASE COUNT         2570 a         1518 c         1324 g         2404 t
ORIGIN      
        1 taaaatttta taacgttctc atttaacaaa acttaagtta ttaagacaaa tgaataaaca
       61 actacgcttt cctcgaaaca aaaaaataaa gcacaaatgc gcatttacgc actcatgctt
      121 tattcgtaca attaattcag ataacggcag cgcataggat gccaataaca cttcctaaaa
      181 tagctcctcc taaaatgtca gtaggataat gtaaaagtaa aaaaatcctc gaataagcga
      241 tcaaacaagc aaataaaaag gctaatattc ctgcaactgg acttaattcg aacaacgcag
      301 ctactgataa aaaggctagc gctgtatgtc ctgaaggaaa agaagaagtc ttcggtttac
      361 ttgtgtaaag gacaagccga cgattttttt cataaggtct tttccgtctt gtcgtttttt
      421 tgataaaaag caaaccaaca aaaaaataga tcaaactgcc gataagaatg gctagaccat
      481 attctctagt atcaggtatc aagactaaaa cagcagcaag gagcaaccaa ataaaaccac
      541 cattagagat attactgata aatttcaaaa aattgatttt tcgactatcc gttttccgtt
      601 gcttggcgaa cttgttgata atactatcat cgattttagc aatcaaacgc aacatgttat
      661 tcctcctttt taagaacctt ttcttgcgta cgatggataa aaagcaggac ttgaaaataa
      721 aacaagaaac tgaaacctaa taaagcaaaa tagattggtc ctgcaaacca gaatgcgaca
      781 agaaggatca acgtgcctgc caaatagaaa agcgcgcttt tgaaatcatt aaaaaacaag
      841 aaaaatgcag taatccaaga agcttctttt tgttccagtt gaagcaagat tttgtaacta
      901 aagagaagcg cgcttaatag aagcaaaaac aacgtagtat acatcaatgt atagccaatt
      961 cccccacccc aatagcggaa aatctgataa tcgatcaata gaagaagaaa ataaacactt
     1021 gctaaactat cttgctttcc ataaagagag aagttctcac gaaacttttt ccagaatagt
     1081 gaaccgagct ttttctcttc attttttttg taatgataaa tcatatacag aactgttcga
     1141 cttgcagttc ctaatgtgaa aacaggcaag acgcacagga accatagtag ctgtaaatgg
     1201 ataagacgtg gaatccattc aatgatttct aagaaataat cattggcttt tttaccaatc
     1261 gatcgattca tcatcctttt gaacctcctg tcaaatccat cccttcaatg aaataacgtt
     1321 gtgcaaaaaa gaacagcaaa atcgtcggga tcaagacaag tgttgctcct gccatcagat
     1381 aattccattg tgtcgttgct tggttctgaa aaacttgcaa gccaatctgt aacgtgtatt
     1441 tttcttgatc ttggatatat aacagaggac ctaagaaatc attccaagct gcgttgaagg
     1501 aattgatcgc aatggtaatc aaggcaggtt tcgtcaatgg aagcatcaaa tgactccaaa
     1561 tatacaaatg attcgctccg tcgatttcag cagcttcgat caattcatta ttgatcgata
     1621 agtaaaattg acgcatcaga aatatattga acgcattgcc gaagaacgat ggaacgatca
     1681 ggggcaaata agtacctacc cagccgatct tggagaataa tacatattga gggatcatcg
     1741 ttacaaaccc cgggatcatc atcgtagaaa gaaccaaagc aaacatcagc tttttcccag
     1801 gaaaatcaag cttcgcaaat ccataagcaa taaaagaatt tgaaaggaca ttaccgatca
     1861 ctactagcac agtgatgaat aatgtatttc ttgcatagcg ccaaaaagga aaggcggcaa
     1921 tcgtatccag atagttttcc catacgattt tttctgggaa aaaggtcgga ggataacgaa
     1981 cgatttcttc cattggtttc aatgaagtaa acaccatcca aagcaatgga atgatcaaaa
     2041 tgattcctat tactgtcatg aggacataac ttagtatttt ttttgtttgc cgattcacac
     2101 tggtcttttt gaaataatcc atcatttgcc gtcacctcca gaataataga cccacttagg
     2161 tgtaagcttc aaattgacaa acgtcagaac caacacgatc agaaacagga tcattgacat
     2221 agccatcgca taacccatat caaaagctac gaacgccttg ttccacatat gcagattata
     2281 aaaaagcaaa gaatttgcag gtccgcctgt tccatttttc gtcatgacat aagcttcttg
     2341 aaaaatttgg aaagaaccga tcgtagaggt gatcacatcg taaaaaataa taggagtgat
     2401 taatggcaaa gtaatacttt tgaactttcc ccaaacgcct gctccatcga tttctgctga
     2461 ttcatataaa gatctaggaa cattttgtaa tgtagctaag taaagtagca ttgctcctcc
     2521 agaactccat attttcatca aaatcaacgc aggtttcgtc cattgtggat caaacagcca
     2581 agctggtcca gggatatgga accagcccag aaatgtgttg atcaatcctg cagatggaga
     2641 aagcagctgc atccataaga tgtagactgc tactcctgat aagactgccg gtaaatagaa
     2701 agctgtacgg aatacactga ttcctttgat tttttgattg agtactgtgg ctaaaaacac
     2761 actgaaaatc gtcgtcagcg gtacattgaa gatgacgaaa taaacggtgt tgtataacga
     2821 tttccaaaat aaatcatcgt gaacgaacat tcttttgaaa tttgctagac caatgaaatt
     2881 catcttggat gtcaaattat aatcggtgaa actaccaata actgaaaaaa gcataggccc
     2941 tagcataaat accaagaacc cgataataaa aggcgagata aataaatagg cccatcttgc
     3001 ttcttttttc tttcggatag ataaacgctt caaattttct tgcctcctct gtacctagac
     3061 agtcaaaaat ggattattga ttttgcttga ctaagttttc tactgctttt tgtgcatctg
     3121 ccaatccttc ttttggtgat tttgttccta aaagaatctg gtcaatctgt tcattcacta
     3181 atgtcgtgta atccggagca tctaaaggaa ctggtgtgat caccgtattt ttaaagtttt
     3241 cgtccgccat ttgatagatc atttttcctt tatccgttag gttgtcatta ttcactaaat
     3301 tctcattggc tgtcttgctc gccatgatat caaaactttt ttcgccgaat ttttcttgta
     3361 cttctggaga agaaagatat ttgatgaatt cataagaggc ctcaggatct tttgctccgt
     3421 atggtacttc caagacgaat ccgccacccc acgaccaatg gcctgaacct tcttcttttt
     3481 ctgggagctg catcacgcca aaatcaaaat catccggtgc attttccatc aaactagagt
     3541 aataattgat attttgggca cgcatggcta ctaatccgga aataaatgga tcagatatac
     3601 cagacccaaa ttcggcttct aatttattga tcgtattttt cccataataa tcttgccagt
     3661 cttggatcca ctccaaggct tccactttat ttttagtatc aatcttgact ttttcatctt
     3721 tgtcaaacca actagtcccg ttatctgcgt ttagcgccca tacatcagcc cctaaattcc
     3781 ataatggata aaaaccgatg cgtgtaaatt cttttccttc ttttttatct agtttacgtg
     3841 ctgtttcttc taattcatcc caagttgtcg gtagatcttg ttcactgatt ccagcttctt
     3901 taaataactt tttattataa aaaatgactt gtgtatctgt attgaatggg atcgcataag
     3961 gatcaccttc atattctact gtatcccaaa gctgcggata aaaatcctta gaagtgtctt
     4021 tttctacata tttggagaga ttagttgctt gctgtgcttc tgctctttgg gcaacggaat
     4081 ttatgtcctg tacaataaca tcgggtgcat ttccagctgt tattgcagat aaagatttag
     4141 tccagatgtc accccatggc tgatacttat aatttacatg gatctcgttt tgtgattgat
     4201 tgaaatcctc aatgatttct tcgatagctt cttttcgtgc accggatccc caaaaggacc
     4261 agaaatcaat attttttctt ccatcacttg ttttttcaga agaatttgct gcacttgaac
     4321 tggcatcgct tgcattactt gatccgcatc ctcctaataa aaacaatgct gaacttgcca
     4381 gcaaccctgt aaacaataat tttttcatat tttacctcca aattttaatg taaccgatgc
     4441 cggttttaat ataacgatat cattttgttc tgaaaataac aatttataag gtatgattct
     4501 aaaaaaatta ttaatgatac caatacttta tataattcgc gagcgctttc tttgaatttt
     4561 ttaatctttc gtagcagaat gagaatgtat taaaaataga ttaggaggaa ttttgatgtt
     4621 tagacaccag aaagaattgc agtttgaagc aaaaccagat cgtccgaatc cattaatcgc
     4681 gaaatatctg caggagctga tcggcggcca atatggcgag atgagtgtag ctatgcagta
     4741 cctgttccaa ggctggagct gccgaggaga agaaaaatat aaagatatgc tgatggacat
     4801 tgcaacagaa gaaatcggcc acgtggaaat gttggcgaca atggttgctc gtttattaga
     4861 gggtgcgcct gttgaagatc aagaagcagc gatgcaaaaa gatccaacaa tcggtgccat
     4921 tatctctgga atgaatccac agcacgcgat cgtagctggc ttaggacctc gtcctgctga
     4981 cagcgtgggc aatccatgga gcggtgccta tatcattgcg agtggcaact tgttagcaga
     5041 ctttagagct aatttaaatg ccgaatcaca aggaagactt caagtagcac gtatttacga
     5101 aatgatcgat gatcgtggcg taaaagatat gctgtctgtt ctccttgcaa gagatagcta
     5161 tcatcaaaat ctatgggaat gtgcagtgaa agaattagaa gaaaaagaag gttcaatctt
     5221 agtcccaagt actttcccgc gtgacaaaga acgaaaagac attgcttatg acttctataa
     5281 cttctcagag ggagaccaaa gtagtgaagg ttcttgggct agtggcaaag cactggatgg
     5341 cgaaggcgaa tatcaatggt tgaaagaacc taagatcgaa ggcgctgcac caaaattaga
     5401 accagtaaca ccaaaaatgt atgggacacc tccattcaag aaataaatat ctacccgtag
     5461 aaacacccgt tttttgttcc acacgctttg tgagcaaaac tgactaaagt cattaatcat
     5521 aaccaccctt caaaagggtg gttttctcta gagctataag ctcaaatcac cggccagcgc
     5581 ctaaagacgc tggctttcat attttatgtt caagccatat tgcctttgcc agttttgccc
     5641 acctcttaaa aggtttatgt actactcctt agcccttaaa aggatcgtct tactctcgta
     5701 cactcagctt atctaacgca ctatcatgtt tttcttgttc ttgaatgtac tttttgatgg
     5761 tcgcttcatt ttgtcctact tttgtcacat agtatccttc cacccaaaag tgtcgatacc
     5821 caaatttata ttttagattt gcacaatctc ttgatgatct cctgaatatt ggctcgatat
     5881 tgattataaa ttatttttct tctatacttt ggtgtgaata caatgtggta tttacacatc
     5941 tactttgtac gggctaaact atttcccttt attgctccac ccgcatagca agtggttttg
     6001 tatttcaatc gctcggcgcg ctcaattagg tctaaagaca ataaatctaa aaacccgctg
     6061 cttcactgac agaaacaacg ggttttcaga aaaaaatgaa atgaaaaaga tctcttacgg
     6121 caatctattt tatttttttg attttttata aatggaaaat gcttcaagaa gtccaagaaa
     6181 taaagccgta taaaaacaag aacctatgaa atctttattt attagatatt caacagaaat
     6241 cccgataaca ctagccaaca atatatttat cagaagtgta aaataaggat gctttttata
     6301 gaatagaact agtgttgttt tcatagactc tcctttcttc atatttccaa ttaatcacaa
     6361 attcacggca ttactagaaa aattaaaggg gcgcagaagt ggaaaaatac tcttcgactt
     6421 tccctacaaa ccaggccatc ttgtgtttaa taactatacc aaatctaagg tacctctaaa
     6481 tctaaattcc atttgtggcg gtgacctcca atttttaact cttctatctc ccatccagta
     6541 tacataacct tgatacttcg accacttata tatcgaacac acaaatacct atccattgca
     6601 ggagtactac ctaacttagt tgctacttgc atctaaataa cagctgaaaa gataggactt
     6661 actcttagta aagtatctga actattaatt tttaaactat catttttatc ctcaactaca
     6721 tgcacatctg ttgcaacttc actttctgtc tcatccagat aaatgattaa ggaatcggga
     6781 attgcaatat cttcagctga cgctggaata gttgagctaa attagtcatc atatctttat
     6841 tataaaataa ccaacagcaa aaaatacaat gaaaaacaaa cttggaacta atgtatcatt
     6901 aataaatgtt gaaaaattca gcaactcatt actgtatgat tttagcagcc caaccaacat
     6961 ataccctata aaagctaata agatggttag gaatccttta gaatttttac tcattcatag
     7021 acacttcctt aaataacttt tattatagaa ggtattgtaa ataataatat tgacaaaaag
     7081 tgactcaaaa taaaaaaaaa cttacattat actggaatga actgtattga tagtgtacct
     7141 aaaagctcta ctgacataag atcgtgtaat cggtaaagta attttattaa ttatacggga
     7201 tgtaatcaat tagagaataa actaatcata aaaggtactt ttacattcat aattgtcgct
     7261 ttgagattct actttggttc actatctgac ccaattgcac attttcgatc attacaagta
     7321 ttggtgtcgt tagaacaact aaataaaaac tagcggtaga tttttcagaa tctatgctaa
     7381 tttctgtatg gatgttccag tgatggcaaa tttctctttt cattgttttt cttgtcgcat
     7441 gatatgcctt tttgtgttat tcagttaaac tatagaaatt ctttcctaga cagatcttga
     7501 tcccaaattt tacgtttttg agcatcgata tagtttaaaa tgacattact catatctaaa
     7561 gagtttagat tttttaggac taaaactgtc agttgttctt tttcttcttt ctgttaaagt
     7621 attgtcgcga cttttcgtat acgttaagat atgtcgtgaa aaaacaagtt ttgtgacaca
     7681 aaaaaacgaa tgacttgagg aaatcacctc aatcattcgt ttaaaatgct atatattctt
     7741 tagtttaaga tacggaaatt aatgccatcg accacactag tagaatgaac gacatttact
     7801 tggccttttt ttaatt
//
LOCUS       DAXWOO010000131         7801 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.133, whole genome shotgun sequence.
ACCESSION   DAXWOO010000131 DAXWOO010000000
VERSION     DAXWOO010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.133"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            90..791
                     /locus_tag="ACGE70_002211"
     CDS             90..791
                     /locus_tag="ACGE70_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFF8693826.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1363..2379)
                     /gene="tsaD"
                     /locus_tag="ACGE70_002212"
     CDS             complement(1363..2379)
                     /gene="tsaD"
                     /locus_tag="ACGE70_002212"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFF8693827.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2376..2840)
                     /gene="rimI"
                     /locus_tag="ACGE70_002213"
     CDS             complement(2376..2840)
                     /gene="rimI"
                     /locus_tag="ACGE70_002213"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFF8693828.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2847..3389)
                     /gene="rimI"
                     /locus_tag="ACGE70_002214"
     CDS             complement(2847..3389)
                     /gene="rimI"
                     /locus_tag="ACGE70_002214"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFF8693829.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3373..4095)
                     /gene="tsaB"
                     /locus_tag="ACGE70_002215"
     CDS             complement(3373..4095)
                     /gene="tsaB"
                     /locus_tag="ACGE70_002215"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFF8693830.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDKIIVPMFDARRKNVYAGAYRWKENRLEIVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFYEIKEILPSAMINTVPAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4286..5266)
                     /locus_tag="ACGE70_002216"
     CDS             complement(4286..5266)
                     /locus_tag="ACGE70_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8693831.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5290..6774)
                     /gene="galT"
                     /locus_tag="ACGE70_002217"
     CDS             complement(5290..6774)
                     /gene="galT"
                     /locus_tag="ACGE70_002217"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFF8693832.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6786..7775)
                     /gene="galE"
                     /locus_tag="ACGE70_002218"
     CDS             complement(6786..7775)
                     /gene="galE"
                     /locus_tag="ACGE70_002218"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFF8693833.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         2175 a         1690 c         1382 g         2554 t
ORIGIN      
        1 ttgtttctaa tgttggtatg ttaaaacttt acaaattctt taaaaaaagt gacaataaat
       61 atagtgaaca ataaggagag gactttttaa tgcgtgttaa taaaatcgat tttcactttt
      121 caaaactcag atatcaacct gattttgaac atttctcgga tgaagagttc caacagatca
      181 aggaacatac tgttcttcgt tcttataaaa aaggacagat ccttttcgac gatggtgatc
      241 ccagacaacg gttgtatatc ttgaccaaag gactcgttcg tttagaacgt tatgatgaaa
      301 gtgcaactta ttactactat gactatgtga atacaaataa cctttttcct atgtcaggaa
      361 tgtttggaga agagacctac acttatacag ctcaagcgat gactgatatt gaaacattct
      421 accttcctgt ttcctactat gaaaaattag cacgaaaaaa tcccgaacag ttgatctata
      481 tgatacaaaa aatttctaaa gtagttgaaa tacatgaatt gcgtatccaa atcggattga
      541 cctctagcgc ctttgaccgg gtaaaacaaa atttattcat tttgaaagaa gaattgggcg
      601 taccaacaaa agatggtcat atcaccattc cttatccgat caccttaaaa gaattagcag
      661 tgaatagcgg cacgacacgt gaaactgctg gacaagtaat caaacaacta cgtgattccg
      721 gactgttaga ttatgacaaa aagcgtttta ctttctgtaa gacagctact actccagcta
      781 tgagagaata acgcgaacaa aaataagcag tgaccctgat atttatcagg gtcactgctt
      841 attttcctcc ctaaaacttc tgaaagatcc ggcagtttta gatcactggg caagactcaa
      901 agattttcta tgtgtgtttc agttatgtaa tattcactgt ttgcaaaaaa tactgataga
      961 ttaactagag gaagcgtctt gtcctcatcc gacctagatt attcgatatt cctcgacaat
     1021 aaacgattaa tcagtgaggg caatcagagg aacggataat gatgaataag ctgtaaaaaa
     1081 tagggctttg acaaaagcgt gtcacagccc ttatttcgat taaacggtgt gtacctgtca
     1141 actcttcggt cacaagtcat aatgttcata aacatgagga gctgtttttt gaaattgttc
     1201 cttgtgaatc agagttaaac gacaggctta cgaactcttg attaaacggc gttcaaaagc
     1261 ttgcatacgc ggtaataaga acagccaggt caaaaacatg agaagccgtt tttcctgggc
     1321 tgttcttatt acttgatgca gggcagattt ttcacaacct ctttattcag ataaaaccaa
     1381 gcttggatca gcattcaact gatagctagc aaaatttctt ttttgcattt ccacatgtgc
     1441 tgctgctcca atcattgcag cattatctcc acaaagtcga agagggggaa tcactagttc
     1501 tacctcaggg agtttagttg ccaaagcatc ctgcaatcct tcacgcaacc cttgattggc
     1561 cgctacacct ccagctacaa tcagttgctt gactggataa ttttgacaag ctcgaagtgt
     1621 tttagtaatc aatacatcga tcacacttgc ttgaaagcta gctgctaaat catttttgtc
     1681 tagatcttct ccacgctgtt gtgcattatg caccaaattg ataaatgcac tttttaatcc
     1741 gctaaaactg aaatcataat tatcttcatg gatcatcgct cgtggaaaat ggtaattatc
     1801 ttttccctga tgagctagct gatcgatttc ttttccgctt gggtaagaaa gacctaatac
     1861 tcttccaact ttgtcataag cttctcctgc tgcgtcatct cttgtctccc caatgatttc
     1921 atatgatcca tcttcttgca tatagactaa ttctgtatgt cctccgctaa caagaagtgc
     1981 catcaatgga aactggaatg gtttaaccag acgggctgca tagatatgcc ctgccatatg
     2041 gtttacagga ataagcggca attggtgtgc ccaagcaaaa gccttagctg cagaaatacc
     2101 aatcagcaac gagccgacaa gtcctggtcc ataagtgaca gcgactgccg ataaatcttc
     2161 tgccgacaca ccagcttcta ccagtgcatc ttccaagcat aaggtgattt gttctacgtg
     2221 atggcggcta gctacttcag gaactacgcc accaaaacgt ttatgactgt ttatttgaga
     2281 agcaacgatg ttagataaga tctctgttcc atttctaacg accgctacac tcgtttcatc
     2341 acaacttgat tcaatcgcca gaatcaattc ttcattcatt tcatcgtctc cgtttttaac
     2401 tttctgctca taatgatcgc atcttccgtc ggattgcgat aatatgattt ccgtttacct
     2461 aatatacgaa acttttctga ttcataaagt tttcttgctg cttcattaga tagacgtact
     2521 tctaaaaaaa catgcagcga atcttttatt tgttccgtaa tcagtaattg taacaaatgc
     2581 cgtgcatgcc cttttccttg ttctgaaact gcaacagcaa tatttgtgat ctcgacttca
     2641 tctattactt tcgagtatcc aataaagcca cagatctggt tatcttcaac taaaagcaga
     2701 taatcagagt gaggctgttg gatgtctgcc aaaaactgca ttttcgtcca aggtgaacca
     2761 tgtggataag cattttcgct aatcatccat acgcattctg ccaagtacgc ttcatcaaaa
     2821 tctttttttc tgcaaatcaa ctaatctcac tcattttcta aaagttttat catatccaac
     2881 gcatcttctt ttgtttcagt gtaatatctt tttttgatcg ttgttgcttg aaaacctaac
     2941 ttccgataaa gtctttgtgc atcttgatta ctcatacgca cttctaatga catcgtctca
     3001 cagcgattca ttatagcaaa gttctcgatt tcgtcaataa gcagcgaacc gattcgttta
     3061 cgctgatatg ccggtcgaac ggcaatattc gtgatatggc aatctaaacc gaacgctctt
     3121 gaaccgataa aaccaatcat tcttccatta tcaaagatac ctagatatag atgaggaagc
     3181 ggtgacttca attctgatat aaaggcactt tttgtccatg gcaattcgcc attatagact
     3241 tctcgttcga catccagcag ctctttgata tcttctgtag tcaattctct tagtacgtaa
     3301 cgacgatcga tttgttcgat tgttttttct gtataaatca ttttatcttg aaaaaagctt
     3361 ctggcatacg ctctaaattt tttcaacata actttcgtct ccaggtgtgt gcgtctccaa
     3421 ccatttttct tctgcttcta cccgttttaa atagcgagga agaaaagctt gtttctctgc
     3481 aactggttca gctaaagcac ctaattgagc cacagttaca ccacttggaa tatcccatgc
     3541 tggaactgta ttaatcatcg cactaggcaa tatttccttt atttcataga agaatttgtg
     3601 acaatcactc ccgacgaaat aaatagcttc attcagcttt tgtagttgtg tgaacaaatc
     3661 agaagcagaa atatgtacat cttcaagtac aatttctaat ctattttctt tccagcggta
     3721 tgccccagcg tatacatttt ttcttcgagc atcaaacatg ggcacaataa ttttatccac
     3781 accgatgcaa ttagccgcga tggttttcag actagatact cctactagct ctttatctaa
     3841 tgtataagca atcgtttttg ctgtcgttac acctatacga agccctgtat aagaaccggg
     3901 accatcagaa accgcgattc ggtcaatgtc ttttggactt atttgaagat ctgcaaacaa
     3961 ttggtcgatt gcaggcatca gtgttacact gtggttgcgt ttgatatttg tttgtatttg
     4021 tccaagaatt tgttcgtctt ctaccacacc gatcgccaaa gtttggtttg cagtatctat
     4081 tcctaatgta atcatatatt tgatcctttc tgctatgaca ttctcgcctt ccattgtagc
     4141 atactttttt attctgagga tgaaaatacc aaaggcaaaa ctagtacaat gatacatgat
     4201 tatctgctgg cgggcaaatt ttgccccttc ctgaaacaca aaaaagctgc ggtatcactt
     4261 catttcccca cagcttttcc atcttttatt tcgttgattc tcgaataatc aactctgttt
     4321 ccacaataat ctttcttgaa accggtgctt tggaattact taattgaaga atcgtctcca
     4381 ctgctagctc tcccatccat tcggtataaa cttttacagt agaaagagag ggcgttacat
     4441 atttcgctac actgacatca ttgaaaccca tcacagaaat atcttctggt accactaaac
     4501 ctttttcttg aatcgctttc agtgcgccaa tcgctaatgc atcattagct gcaaaaagcg
     4561 catcaggaac atgcggattc gtttttaaat actgcttcac tgcttgatat cctgactcga
     4621 cagaaaaatc tgcttcgata attggacctt cgtcgtagcc tttttctgcc aaaacttgtt
     4681 gaaatgccat aattctggga tcttttagcc gctgatggtt tttcttcgta tattctgttc
     4741 cagcaagcat agcgatatgt tgcgctttat gggagacaaa atgttcaatg actttttgaa
     4801 cacttgactt aaagtcgaca actagagagt cgattccttg agaagtgccg tcagaatcta
     4861 ccaaaagcaa ggcattttgg gatcctttca tttctaataa ctgagcttcg tcgaattttc
     4921 ctagtgcgat cgttccatct gtcggaagat cactaatgga atcccacgtt tctttcaata
     4981 aagtaacgtt tgcttcttct gccttttttt cgattcctag acgaatagat aaataataaa
     5041 gatcttcaag ctcttccgta tcgtcgtacc actggatcag gcgaagcgtt tgcttagtat
     5101 ttgctgcatt tttacgatgt ttcgtataat ttaagcgttc tgctgcttca aaaatttttt
     5161 gctttgtggc taagccaacg gaaagatcag gatcatagtt gagaactctt gaaacagtag
     5221 caggagaaac tcctgccaac tgcgcaatgt ctttaattgt tgccatttgt ctctcctcct
     5281 tttttacgtt tataaaattg cagtaaatcg ttcaaacgct gcctgtcctt tttcatctcg
     5341 cttgaatact ccagcgtctt ccagtacacg tgcaaaaacc tgacctacag atttttgtac
     5401 gacatcggtg accgtatctt tcgtcaaatc aggatgttct ttttttaatt gttctgccca
     5461 cggacgatga tattcagcca tctggttttc ttctcctaat aagaaatgct ctacttctac
     5521 tagttctggt ttcaaacgag gtggtaaaac agcaagcccc attacttcga tcaacccaat
     5581 gttctccttt ttgatatgtt ggacatcttt atgtggatga aaaatcccat caggatgttc
     5641 ttctgaaaca ttgttgtctc gaaggacaag gtccaactca aagtaattgt cccttcttcg
     5701 tgcaattggc gtaatcgtat ggtgtggtgt accatctgct gaaaaagctc tgatagatac
     5761 tgtttcatcc gaatatgctt tccacttttc caatactgag ttagctgctt caatcaacaa
     5821 ttttttctct gttgattgca aacggatcac agacatcggc catttcacga ctcctgcgat
     5881 gacttgcgga taatctgcta gtttgataag tttttctatc ggtgcttttg ccataggaaa
     5941 ttcatgacgt cctgcctgat aatgatcatg agaaagaatc gatcctccaa caatcggcaa
     6001 gtcagcattc gaaccaacaa agtaatgagg caagacttca gtaatccgaa gcagtctagc
     6061 aaatgtttcc tttgtgattt tcatcgggcg gtgttgttct gataaaatga tcgaatgttc
     6121 attgtaataa gcataaggag aatattggaa tccccagctt tcaccgtcta gattcattcg
     6181 aataatccgg tgattggtgc gggctggata gttcaagcga cctctatatc cttcgttttc
     6241 catgcaaagc atgcaggtag gatagtctac ttttaccgcc tcgcgttctg ctgcgatttg
     6301 ttttggatct ttttctggct tagataaatt gattgtgatt tccagttcac catactccga
     6361 ctctgccgga aatttgatgt tttttgcgat ggctcttgtt ttgatgtagt catttttctt
     6421 acttaaatta aagaaatact cagttgcttc ctctgggctt ttcgaatagt gctgtgcaaa
     6481 gaatgcattg acgactgaag gtggcggtgt gagaaaatcc attaattgcg cttcaaaaat
     6541 ctcttgttct gctggcaatt ctgaaagaac accattttct cttgccttag cgacaagctg
     6601 atccaataag tctacagatt cttccttaac tggtcttact tcaatgcttt ctagagcatc
     6661 ttctcctacc atccctaaaa gacgattctg taaatataag cggtccattt ccatccatcc
     6721 gccagcttcg atcgcaagtg tggcaaaatc acaaatcgtt tgactgattg acatatctca
     6781 ttcccctatt cttcataacc atttggatgg ctgacatgcc aatcccatgc tgttttgata
     6841 attgctttga tatctgtgta ttcaggtttc caacccagaa cctctctcgc tttttcactt
     6901 gaagcgacta atcggctagg atctccagct cttcttggtg ctatttctgc tggaatttct
     6961 tttcctgtca cttctctagc tgcttctagc atttccttga ctgagtatcc tttgctgctg
     7021 cctaaattga agaagttgct ttcatttcct tctttcaagt attccaaagc taaaatatgt
     7081 gcagcaatca aatcttctac ttgaacataa tctctgatac atgttccatc tggtgtatca
     7141 taatcgtctc cataaacagc caatgctttt ctttgaccaa gagctacttg taaaatgatt
     7201 gggacaagat gcgtttctgg tgtatgatct tctccgatag atgcatctgc ttttgctccg
     7261 gctacattga agtagcgcaa agcgacatag cgcatgccat atgcttggtc acaccacttc
     7321 atcatttttt ccatcatcaa tttactttca ccatacggat ttttaggatt agctggtgta
     7381 tcttctgtga ttggagactc ttttggttct ccgtaagttg ctgcagtaga agagaacacg
     7441 attttattca cattgaactc gtgcatgact tcaagtaaga tttgcatacc gtatacgttg
     7501 ttgttgaaat acatcaatgg tttttcgaca gattctccta ctaaagagct tgcagcaaaa
     7561 tggattactc cttcaatcgg ttccttttca aatacggaac gtagaaattc tttatcccgg
     7621 atgtctcctt cataaaaatg agcatctgga tgtactgctt gtttgtgacc tgtgagcaaa
     7681 ttatctacca cagctacttg ataccccttt tgaactagct ggtctacagc gtgcgaaccg
     7741 atataaccgg ctccgcctag tactaaaatt gtcataaaaa acactccttt gattagtaaa
     7801 a
//
LOCUS       DAXWOO010000132         7760 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.134, whole genome shotgun sequence.
ACCESSION   DAXWOO010000132 DAXWOO010000000
VERSION     DAXWOO010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.134"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(552..1898)
                     /gene="gor"
                     /locus_tag="ACGE70_002219"
     CDS             complement(552..1898)
                     /gene="gor"
                     /locus_tag="ACGE70_002219"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFF8693834.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(2032..3285)
                     /locus_tag="ACGE70_002220"
     CDS             complement(2032..3285)
                     /locus_tag="ACGE70_002220"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFF8693835.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(3291..4214)
                     /locus_tag="ACGE70_002221"
     CDS             complement(3291..4214)
                     /locus_tag="ACGE70_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFF8693836.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(4435..6927)
                     /locus_tag="ACGE70_002222"
     CDS             complement(4435..6927)
                     /locus_tag="ACGE70_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFF8693837.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(6934..7401)
                     /locus_tag="ACGE70_002223"
     CDS             complement(6934..7401)
                     /locus_tag="ACGE70_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFF8693838.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(7537..7610)
                     /locus_tag="ACGE70_002224"
     tRNA            complement(7537..7610)
                     /locus_tag="ACGE70_002224"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7574..7576),aa:Arg,seq:acg)
     gene            complement(7668..7741)
                     /locus_tag="ACGE70_002225"
     tRNA            complement(7668..7741)
                     /locus_tag="ACGE70_002225"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7705..7707),aa:Pro,seq:tgg)
BASE COUNT         2161 a         1691 c         1247 g         2661 t
ORIGIN      
        1 aaatcagtgt tttgtcctga gtattagaga aacaaatgga aaaatagttc ctcatatttt
       61 gccgtttttt gagctaatac cgaaggacag ctgattgaac actatttgtt cggaaaaagt
      121 gactaactat gacacaattt ttttcacagt cccttttttc acttgatttg attcgtacca
      181 gcgaacttga cgagaacttt cttggtttgg taactatatg gtattctttt aacatcctcg
      241 tttgcttctt ctaatttatt tatacaaagt cacacttttt taaacatctg ttcttcgata
      301 catttctatt tcttttaaac atcgatacca atattttgaa cttaagagtg cataccaaat
      361 ggattcattt actcttcgtc agttacgaag ataatcaaaa tctccaactt ccctacttat
      421 ttcgttttga gaattatcga agcaatctta cataaataac taaaaaacaa aaaaccagaa
      481 aatcagtata ctgattttct ggttttacat ggtttgttca ataaaaagct gttggaactg
      541 ttaatattaa attatcgcat cgttacgaat tcttccgctc ctgttggatg gatggctact
      601 gtattgtcaa agtcttcttt agttgcaccc atcttgatag ccacggcaaa accttgcagc
      661 atctcatcga caccaacacc aatagcatgt aagccgacga ttctttcttc ttttccgaca
      721 cagatcaatt tcatttcgca tttttggcga taatcattta atgcaaagta cattggggta
      781 aagcgtgaac gataaacttt caattcattt tcgccatatt tttccaatgc ttcttcttct
      841 gtcaaaccaa ttgttgcaat tggtggatgc gtaaagacga ctgtcggaac aagtttatag
      901 tcaagataag aatctttctt tccattaaat aaacgttctg ataaacgtct accagctgcg
      961 attgcaacag gagtcagatc taatttaccg atcacatcac caacagcaaa aatatgttca
     1021 tccgttgtat tttggtattt atctactttg ataaaccctt ttttgtccaa ttccacattt
     1081 gtattttcca gacctaattt ttcggtattc ggtacacgtc caccggcaaa aagtacacaa
     1141 tcaccagtta aggtttcacc attttcaaat agcaccgtat attcgtcatt gtttttagta
     1201 atttctttag caatatgatg tgcgtaagtt tttattccag cttcttggta catttggatc
     1261 aaattgtccg aaagcatttt atcaaacgtt ctaagtggtc gttcttttcg atatgcccac
     1321 atgacttcac tacctaaacc acgaaataca cctgaaagtt cagccgcaat gtatccagca
     1381 cctaaaacga ttgctctctt aggtaactct gttaattcaa aaaatccatt tgaatcaatt
     1441 gcgtattctc caccaggaat ggtcatttta cttgcacggc ctccagtagc gatcaagatt
     1501 ttaggagcac gataggtttc accgtttact tcgatcgttt gactatcgat aaaagtagcg
     1561 taacctttta tagcctcgac atgattgcta tctaatcctc tttgataagc accgtgtaga
     1621 aaatcaatat atttttctcg tctttctact agaccggcga aatcaacatt ttttaaatct
     1681 gcttggatac cgtaacttga ggcatctcgt tcaattgttt ccaaaatcgt acttgcttgc
     1741 cacataactt ttttaggtac acaaccgacg ttgacacatg ttccgcccaa ttcattcgct
     1801 tcaatcagca aaacttttgc gccatgcatc cctgctctgt ttgctgaagc aataccgcca
     1861 gagccgccac caattacgat gtaatcatat atttgcattt tgttcctcct tattgaagag
     1921 atcaatcaaa acctttattt aacgatcctt tttctacaga aaacaagtgt aacacttttc
     1981 ttgaattttg tatgcgaaga tgcttaaaac agggatatat cagctttttc cttatttacg
     2041 ttttcggatc atatctccag gtttcaccgg ctgtgcgact ggcatagtta aaatcatcat
     2101 tggatttggt gctcggtcaa tggattcgcc ttcttcatta tgcataactt caacggtttg
     2161 actgaaatga ctgaatcctg gaccataaaa ttcgatttca tctcctacag aaaacaagtt
     2221 tctctgacgt atcgtagcaa tctgtgttgc ctcatcataa gataatactt caccgacaaa
     2281 tttatatact gggattttac gacgttcacc gaataattgt tcgttttcag ttggtgtatt
     2341 gtagtaaaaa cctgtagcta attctcgttg tgctactttc cataactcgt cgatccattc
     2401 ttgcttgcag acatagtttt caggatcttc catataactg tctacagccg ctttatagac
     2461 atttgacacc gtagatacat aatggatcga tttcattctt ccttcgatct tgaagctgtc
     2521 tacgccgttt tcaatcaatt ctggaatatg ttcgatcatt gacatatcca ctgcactcat
     2581 cgagaattct tcttccactt cgcctgtatc agttaaactg tttcgttctt gaccaaaagg
     2641 catatcgaac aatccatact tccagcgaca agattgagaa catccgccac ggtttgcatc
     2701 ccgcatcgac atatggttgg acaatgtgca tcgaccagaa taagagatac acatggcacc
     2761 atggataaat gcttcgattt cggtatccgt atttttacga atctctgcca cttcctccat
     2821 tgaaacttca cgagctaaaa cgacacgttc caacccttct tctttccaga attccaatgt
     2881 ctcaaaatta gttgctgaag cttgagtgga taaatggatc ggcagacctg gggcttctgt
     2941 cgcacaaatt tcgatcaaag ctggatcaga aacaatcact gcagaaatac cgacatcacg
     3001 caaactgcgg aaaaattccc ctgctccatc ttcatttcct tcatgggtga ccatattagc
     3061 agcaacgtat accttcgcat catgttcttt tgcaaaagca actccttcag ccatttcttc
     3121 ataagtaaaa ttacctgcac ggcttcttaa accatatgca ttcccgccaa tatatactgc
     3181 atctgctcca taatggatcg cggtttttaa cttttccagt gttcctgctg gcgctaggat
     3241 ttctggtcgt ttcaattttc tttttactgt catttctttc atcatactcc ttatttgata
     3301 gcagaaggat caatataaaa gaatcctgta tctaattcac ggttttcagg atgcaattta
     3361 ctgatttcgc tagtcatttt ttccgctttt tcttgatccc agctaccttc taaaatttgt
     3421 ttttttgctt cggcgaatag tcgagtgatg tttacgaatt tttcgcctgg agtaaataat
     3481 ccgtccaatt tccatgtaca gtaatgatgc tgataaagtt cattcaattc attcgagaga
     3541 ttcagatcat tgctggcaaa aatatgtgtg ccatgactat cttcataaat agaataatgt
     3601 gtctcttctt ttttaggttc cgagataaaa agacctcttt cacgatcttt ttgttcgtct
     3661 tgtttcgtat agttatagta attctgtagc agtggacgtt tggattgatg gatgcatgta
     3721 gcaccataca caagggtttc tacaggaata tcgagttttt cttccatcgc cttcatctct
     3781 tcaaaaggaa cttcacgagc aagtactgct ccaactgcgc cttttttcgc ccagaaattg
     3841 atttgacgtg cgctcgttac taatgtttca gcatcataaa tatacggtat acgcaattca
     3901 ggattttttt tcatcgtata aataatacct gtatcaccaa ctgtgatcac atctacgttt
     3961 atttctttta aaaaagccaa atattcaggt acatttttca ttttttcagg atgaatgatc
     4021 ccatttacag caatattcgc ttttttacca tacttgtgtg ctaattctac caattgtcgc
     4081 tgttcttctc gtgtaaatga agcaggcaga cgcaatccaa attgctcttc accgaaatat
     4141 agtgtatctg cacctgcagc aagcaactgt tctgcttgtt caatagtttc tactgtggtt
     4201 atgagttcga tcataatttt ctcctcctac aacttcataa tagtagcata gctctaaaaa
     4261 ctttccaata cttcttctat ttaaagtcgg atttttgaaa attatttcac aaaaaatcaa
     4321 aataaagttc gatatattct gtccaaatat aaaaagagat tgtgacaaaa atcttgtcac
     4381 aacctcttaa tcaagtctta ctattataaa aattcagcta cacaggccaa gtaactatag
     4441 ggaacctact gtttcctttt taggtccgcg aacattcaat gtgatcttac cttttgaagc
     4501 acctactgtt acgtggtcac ctaaacgaat ttctccgcca agcaaagcct cgctcaatcg
     4561 atcttcaatt tctttttgaa gtgcgcgacg gattggacgt gcaccatatt caggatcaaa
     4621 tccagctttc ccgatcacat cgattgcaga tggtgtgatt ttcacttgaa tatcttgttc
     4681 tttcaatcgt ttgataatcg ctttgctcat gatttggacg attgtatgga tttcatcttt
     4741 actcaatgaa tggaagacga cagtctcatc gattcgattc aagaattcag gtcggaaagc
     4801 tttctttaac tcttctaaga tacgtttttg catggctttg tgatctttag taacatcttg
     4861 gacattgaat ccgacatttt tttcttctcg aatttgtgtc gctccgatat ttgaagtcat
     4921 aatcatgatt gtatttctaa agtccacttt tctaccttta gaatctgtta aatgaccatc
     4981 atccaatact tgcagaagta aattgaaaac gtctggatgt gctttctcca cttcatctaa
     5041 gagaatcact gaatatggtt tttgacgaat tttttctgtc aactgtccac cttcatcata
     5101 ccctacgtat cctggaggag acccgattaa tcggcttgta ctgtattttt ccataaattc
     5161 tgacatatca acacggatca gggcatcttc acttccaaac atgacttctg aaagtgcttt
     5221 ggccaattct gtttttccga caccagttgg tccaaggaac atgaatgaac caattggacg
     5281 atcaggatct ttcaatccgc ttcgtgcacg gcggatcgca cgtgaaactg ctttgacagc
     5341 ctcatcttga ccaacgacac gttcatgcag aagtccttcc agttctaaca agcgttcgct
     5401 ttcttttttc tccaactgct gtagagggac accagtccac tccgatacaa ctgtagcaac
     5461 atcttcttct gttacacgat cagaataacc ttgctcctgt tttgcttcaa ttgcagaaac
     5521 tttttgcagc tttttacgta gtgctttttc ttgtctgcgt aattgagcag cattttcaaa
     5581 gtcttgtttt tggatcgctg cttccttttc ttgtactagt tcatcgattt ctagtttgat
     5641 cacagttgat tttgttaagt gatcggtttg atccaaacgg actttcgctg ctgattcatc
     5701 gatcaaatca attgctttat cgggtagttg acgagaagtg atataacgaa cagaaagatt
     5761 gactgctgct cgtacagctt catctgtgat ctccactcca tggtgctctt catatcttgt
     5821 acgtaatcct tgaagaatca cttctgcttc ttcaggagtt ggctcatcta cttgaatgcg
     5881 agcaaaacgt cgctctaatg cagaatcttt ttcgatatat ttttgatatt catctagggt
     5941 cgttgctccg atcgtttgta attctcctcg agctaacgcc ggtttcaaga tattcgaagc
     6001 atcaattgct ccttctgctc cacctgcacc aattaaggta tgcaattcat caatgaataa
     6061 gattacttga ccatcattat agatttcgtc gatgaccttt ttcattcgat cttcaaattc
     6121 accgcgatat ttcgtaccag caaccaaaga tcccatatca agcatcatca ggcgcttttc
     6181 ttgcatgtct tctggcactt cacctaaaat gattttttga gctaaccctt cagcaatggc
     6241 tgttttgcct acaccaggtt cacccaccag tacagggtta ttctttgtcc gtcggctaag
     6301 aatctggata agccgtttca cttctctcga acgaccaaca actgggtcca gcttgttttc
     6361 ttttgctaat ttcgtcaaat cacgtgctaa tgaatctagc gtaggtgttc cttgcggtgt
     6421 ttgccgttta tcttgtacat ttacatttct acgacgattc attccttttc cagcagaatc
     6481 accagctagc cccatttttt gtttcagaac ttgacgggct tttgaaaggc tgatacctaa
     6541 gttgatcatg attcgagtag ccatgatact ttcatctttt aataagctca tcaaaatatg
     6601 ttctgttcca actttttgag aaccagtttt cttcacttcg ctgctagcta aagcaaaaac
     6661 ttgtttcatt cttggtgaat atggaagaaa gactccttta gggtaagaac ggataccacc
     6721 ataaccaatc agatgttcaa tttcttcata aatttccgtt tcattcaaat ccatttcacg
     6781 taatactttt ccagcaattc cattttgctc gaccactaag gctagtaaaa catgttctga
     6841 actaactgat tgatgtttca accgttttgc ttcttcttgt gcaatttcta atactgcttg
     6901 ggctctttca gtaaaaagtt cttccatcta atctcacttc ccatcttcat aacttaaacg
     6961 ttccagaaat gctcggagaa tccgagcacg tgtatgatct tcatattcat tgaaattcaa
     7021 tgtgtttttt gcaattgcac tcaacatcaa gtttccttct ttttttgtaa ttatttggtc
     7081 ttcataaagt ttttgaataa tagcaaaagc atttttctca ctgattgtat catcaaataa
     7141 ctgatttatc tgctcaagta attgtttttt atctgaaatc cttacttttg caattcgtat
     7201 ataaccacca ccaccacgtt tgctttctac tagataacca cgttggatgg taaaacgggt
     7261 attaatcaca tagttgattt gtgaaggaac acagttaaag aggtctgcca tttcaatcct
     7321 tcgaatctcg atcatttcac tattttccag aattttcttt atatatgatt caattaaatc
     7381 cgaggtattc tgatgtgcca tcttattcat tccctttgac taatattgac cttaattata
     7441 acgaatttga taatagaaag aaagaagaac gctttttgtt aaataaatcc tttagaatca
     7501 acaaaaaaaa atacggaata tactattccg tatcgacgcg cccaagagga gtcgaacccc
     7561 taaccttttg atccgtagtc aaacactcta tccagttgag ctatgggcgc atattcaatt
     7621 taaaaaaatt ataaatcatt tgaaaacaaa aagcaagata aaaaaaatcg ggaagacagg
     7681 attcgaacct gcgacccctt ggtcccaaac caagtgctct accaagctga gctacttccc
     7741 gtttaaaaaa taaaaaagac
//
LOCUS       DAXWOO010000133         7724 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.135, whole genome shotgun sequence.
ACCESSION   DAXWOO010000133 DAXWOO010000000
VERSION     DAXWOO010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7724
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.135"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(630..3779)
                     /locus_tag="ACGE70_002226"
     CDS             complement(630..3779)
                     /locus_tag="ACGE70_002226"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HFF8693839.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(3792..4991)
                     /locus_tag="ACGE70_002227"
     CDS             complement(3792..4991)
                     /locus_tag="ACGE70_002227"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFF8693840.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLIDLVVRLNKSTNSSRLPKVEF
                     EDIVAGEGRLNKDVSQKFDNRKGIEFLPNDILYGKLRPYLKNWLKATFTGVAIGDFWV
                     FRAKKSDSDFIYSLIQADRYQKAANDTSGTKMPRSDWKKVSDTVFYIPNDLKEQQKIG
                     IFFKKLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWEERKLGE
                     LGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIERKKWKPI
                     KDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFETINLPR
                     YSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLLKETKKS
                     FLQKMFV"
     gene            complement(4981..6576)
                     /locus_tag="ACGE70_002228"
     CDS             complement(4981..6576)
                     /locus_tag="ACGE70_002228"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFF8693841.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEMWLQGALEVFKHEQ"
     gene            6701..7486
                     /locus_tag="ACGE70_002229"
     CDS             6701..7486
                     /locus_tag="ACGE70_002229"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HFF8693842.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
     gene            7479..7721
                     /locus_tag="ACGE70_002230"
     CDS             7479..7721
                     /locus_tag="ACGE70_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693843.1"
                     /translation="MTNNILISIYPRYMEKIINKQKNFEFRNFYLTSDPYNIFWIYET
                     IPTKALKYKLIVKNPITKLKKNQHYFLGDDEFEQLV"
BASE COUNT         2285 a         1530 c         1257 g         2652 t
ORIGIN      
        1 aaattgatct atcaataatt atcaaactga gtcatataga gacaaaaatt tcaaaaatga
       61 cctgttaaat ggtcatcgaa tatcatttaa aatcaatacg acggagtcag acacggagtc
      121 aaaacaagcc cgctgagagt aaaaaatata cagtcccata ggtaaagtgc cacaggactg
      181 tatatttttt tatttgagat tacctgaaat gattccattt aatttttatc tcattttttc
      241 ctactagtta gtatcaaata ctctcctatt ttctcaataa actattttgg gttacatggt
      301 atctgaaaca gctaaaacct tgtcattata gcaaaaaatc actttatctc cgtttcattt
      361 cagatacatt tcacttaagc ggattccaaa aaaacaaaag aaaaaggaac ccctagttag
      421 cctatgcttt ttattggttc ctctcaaaac acggtcatat aacaacgggg tggggtaaat
      481 gaatgaaaaa ggtggaggaa acattattca atagctgtct taaatacttc gttataaaat
      541 gtgaacaaat aaaagacatc tatccctatt ttctacactt ccacttgact accatctttg
      601 aatcgtacaa ttttcttaac tcttctcgtt tacctcaatt cattctcaag tggaatcaac
      661 tcatcatcta acattttacg ccatgctcgc ttcatttctt gtgggtattt aaatggcttc
      721 gcttctggat gtttggcttt atatccttcg taatccttac tgttgataat tgctttcata
      781 ttaggaattg gatcttcacc tggtagatat tgcagattag aagcatgtaa ttctttctca
      841 gaaacaaacc actcattgga aaagacatga atcaaatcat cactcgcttg tgtaaaatag
      901 cgacgtttaa tgacaaagat atcttcatta ctatctaaag tccccacatc aaatgcattt
      961 aaaatttgtc ggtaaacttc tttgactcct tctgttttat tcaatttagc caaagctttt
     1021 tcaatttctt ctcgtgcatc tgaactattc gcagcataag ttcctagtaa ttgatcaata
     1081 taagttgaat caatatcata caacttagtg gtattttcac tataaaattc gatcgttgaa
     1141 aaatcttctc cttctttttc tggttcttcc tctattaatg aacccttcac ggtctcgtac
     1201 acgccaattt gttcttccaa aagttgaatt tgattgttca aagttggaga tttcaccatg
     1261 tcatcattgt aatcgtcata agtcactaac gattcataag cacgattcaa ttcttgaaat
     1321 gctttgacgt actcgatacg gatttcaagt ggcgtatgtt catcaatgga ttccatggat
     1381 gaaacctctt ttaacttacc atatgcttgt ttgaatcttt ttttggattc ttggtaactt
     1441 ggataaatca aacctgattc ttcttttgct tttgaataca gttttgtcgc atcctcgaca
     1501 ttcttctcca ttgtcgctgg cttacgaaac gtcacaatca atccttttgt cttatcagga
     1561 tacgtacgat tcgtacgtga aaaagcttgg atcaatcctg catactctag attacgatcc
     1621 acaaataggg tttgaatcgt aggggcgtca aaacctgtta atagacgatc cactacaatc
     1681 gctaaatcga tttgtttccc aaattgtttg aattccgctc tttttcgtgc caaacgatta
     1741 ttgatgtctc cgttataacg ctcaatgtct gctaatgacc aggaagtatc ataatactga
     1801 ttatattcct caataatttt ttgcatttct tcttgttggg cacttgaatc tcggctattt
     1861 tcttctaatg aataggtaat agcgattcgt ggaaaatcag gatcctccat cgttcgtcct
     1921 tcacgaatga gctgattggg aaactctttt gttaaccaat caggctcttt cgtcatctct
     1981 ttgatggccc gataatactt tttggccatg tcaatggaac tcgtggttaa gattgcagat
     2041 ttcgttggcc tgccatttcg aaaatcaaat tttgtatagg cattatctgg acggaacatt
     2101 ttatgaatga ctttttggat atgttcatct gcttcaaaaa cagctgggtc caaataactt
     2161 tcttttttga caggttccat ttgatcaatc atacgattga tctgttctga tgaatactct
     2221 gcatatgttt ctacttcttt taatttccga tagattttat tctctagtga agtcggctcc
     2281 acggtgtttt catgctctac ttgaaatcct aagacagagc catcttctag cgcattctta
     2341 atggtatagg tatgcaagac ctctccatac tgatcatggg ttgtacgtgc caattgccct
     2401 ttggcttgtt tccgattctc aggaaaaatc ggtgtacctg taaagccaaa ccaagtggat
     2461 ttcggaaaca tttttttgat ttcttccata ttttcagcac ttaacgctcg atgacactca
     2521 tctactacaa acacgatatg ttggcccatt aatttttgga agcgattggt tccttttttc
     2581 tcttcttgtt tctttgcata tttcaacgcc gcatctagtt tttgacgggt tgtaatgatt
     2641 acaacatttg cattggcatc tgccaacagg gtttcactta attctttggc acttcccgtt
     2701 ccaacgatca agctattcgc tttggcgttc ccggaagaaa tcccagtatt aaattccgaa
     2761 gcaaattttg taaactctgt ggtggtttga ttatctaaat ctttgcggtc cactaacatg
     2821 atcgttcgat cgatccccga ttttcgtgct aaaagcttag tagatacaaa actagtcaac
     2881 gtctttcctg aaccagtcgc atgccacaca taccctgatt gatgtttatt tgcggcaata
     2941 aacaaggctt gaatcgcatg tacttgataa ggatgtaaca ccatgagggt tttattatct
     3001 tgatcttcac tgacgattgt ataatccgca atcaaacgat gagcatccgg gatatttaac
     3061 acttgtttac aaaactcata taagttttct actttttcat tgtctttcgt tcgccaacta
     3121 aataaaaact tagggtgcaa gtctttcggt aaggcatttg caaaataccg agtggtttgt
     3181 tcattggaaa ccacaaacaa ctgtaaggtc gaaaagatat tgttccgaaa cataccttct
     3241 tctgcatatt ttttgatttg gttaaatgcc tgataaacac catcttttgc agttacttgt
     3301 ttcagctcga tttggacaat cggtaatcca ttgattaata aggtgacgtc aaaacgtcga
     3361 tcccgtgctt ctatgttact tccacgtttt gcaatttgat ggaccacttc ataagtagag
     3421 attcctccac caatgtcttg attggaataa aggataagtg agacagaacc caattgcgaa
     3481 tcttcccgtt caatcgtgat ccgagccatc ccattttcac cttttaacca tttcgcagca
     3541 tcaaatgggg ttttcgttcg cagcagtaat tccgttttga tcgtttcaaa ttctttatcg
     3601 gtcaaaggaa aatcattcag ttctgcttga ttatttgaga taattttttg gcgtaaattg
     3661 acccaaaggt cttcttctga ttttagatcc ggacgatacg tccactgatt atgcccttct
     3721 ccaagtacac gaatcaactg ttcttcgact tctgcttcct cacggtgact aattttcatt
     3781 cttcttccct cctagacaaa catcttttgt aagaagcttt ttttcgtttc ttttagtaaa
     3841 tctaacttac gttgatgaag agcgatcgta tcatctagct gtttgaagaa agtaccgatt
     3901 ttttgttgtt catcctttac aggaacctta acactgatat tctcaatatc agaactgtta
     3961 taagaaggca atgcccctac ttgggagtat cttggcaaat ttatagtttc aaacaatatt
     4021 ttaccaaaat agatatccca agtattatta aataaataag ccatggtatt gttatctatt
     4081 aagaaagggc ttgtgaccaa acgttttcta ttaagcatta ttgctgcgcc aactttagca
     4141 aatataatcg ctggcacatc ttttattggc ttccattttt tacgctcaat ttgctcatta
     4201 gatacataat gatttgcatt tctcatttca tactcattac caatgttatt catgtcggaa
     4261 actttaaaaa atggagtacc ttcagaacca ccttgctcgg catcaggaaa tccaattccc
     4321 gattgagttt tacctaattc tcccaactta cgctcttccc aatcttccgt aaaaccagga
     4381 aaccggattt ctggcacttt tgctccgttt tcagggaaca ttttttgtaa gaatcctttt
     4441 ttcgtttcct tcagcaaatc taacttacgt tgatgaagag cgatagtgtc gtcgagtttt
     4501 ttgaagaata tgccgatttt ttgttgttct tttaaatcat tgggaatata gaatactgta
     4561 tctgaaactt ttttccagtc tgaacgaggc atttttgttc cagatgtatc atttgcagcc
     4621 ttttgatatc tatcagcttg aataagagaa taaataaagt ccgagtcgct cttcttagct
     4681 ctaaaaaccc agaaatcccc aatcgctacg cctgtaaaag ttgccttgag ccaatttttt
     4741 aaatatggac gtaactttcc atatagaata tcatttggta aaaattcaat cccttttcta
     4801 ttatcaaatt tctggctcac atctttattc agtctacctt ctcctgccac gatatcttca
     4861 aattcaactt ttggaagtct actggagttt gtacttttat taagtctaac aactaaatca
     4921 attaacttac gctcttccca atcttccgta aatcctggga aacggatttc tggtcgtgta
     4981 tcattgctca tgtttgaaca cctccagcgc accttgtaac cacatttcgt tttcaggtgt
     5041 tgtttgcagg gaagagatag cttctaaaag ttctttttct agcacttgct gttcttgtcg
     5101 tatcttcttg atgtcatttc cgacatgaac catatcaatt ggttcttctt cttcgaaagt
     5161 atctacatag cgtgggatat ttaagttata gtcattttct ttgatttcat caaaggttgc
     5221 caaatgagcg tatttctcga catcttttct ttcagcgtag gtttcgagga ttttttgaat
     5281 gttttcttcc gatagtttat tttgattttt ccctttaaca aattcacgac tagcatcaat
     5341 gaataataca tctcgagttt gacgattctt cttcaacaca atcaccgtcg ttggaatcga
     5401 tgtcccaaag aacaagttcg caggcatacc gatcacagca taaatactgc catcttctag
     5461 taatttttga cggatgaccc cttctgcagc gccacggaat aaaactccgt gtggtaagac
     5521 gatggccatg gtacctgttt ccttcaaatg atagaaccca tgaagaagaa acgcaaaatc
     5581 tgctttggat tttggcgcta atttcccata acgattaaac cgagaatcat ctaagaaagt
     5641 cgtatctgct gaccaattag ccgaataagg tgggttcatt actactgcat caaatgtgta
     5701 aggttcatct gttggccagt ccttattcaa cgtatcccca ttgcggagat tcatttcttc
     5761 ggcgtcgaca ccatgtaaaa tcaagttcat ttttgctaga ttgtatgtcg ttgtatttag
     5821 ttcttgccca tgatatttaa cgttatctgg gtgtgttaag tagttacgta cgtttaacat
     5881 caatgaacct gaacccatcg ttggatcaaa aacactaaag aatcgtcgtt ccttttgatc
     5941 gagcgttacg atttgcgcca tcatatcaga aaccatatga ggtgtgtaaa attctccggc
     6001 tttcttccct gcttctgaag caaattgact gatcagaaac tcatacgcat ccccgatcac
     6061 gtccccatcg tgttctaaaa catctacatc attcaatttc ttgatgactt cggtaatcgt
     6121 cacatttcgt tgttgctcat ctgttcctag tttttttgat tggagatcca catcatcaaa
     6181 taacccatta aattgattgt aggtagatgc caattgcaca aaggctttgt ttagatcgtt
     6241 caattggaac gttgcttgtt ttgcttgatc cgctaagacg ttgaaaagat actctggtgc
     6301 aattgcatat cccaaaatat caacgattgt ggcaatcaga tcttcttttg attcttcatc
     6361 tgaaagtaat tcacgataca acattgtttg tttgcttact gtgtcatatt cctctaagga
     6421 ttcatctgct aaaagaacca cttgttccaa caatcgatcg gataaatatt tatagaaaat
     6481 cagtcccaat aaataatttt tatattctga cgcatccatt ttactacgga gattatctgc
     6541 cgcactaaat aatttttgat ttaattctgc acccaaaaag atacactcct atctcttatt
     6601 taaagttgct atataccctc ttattttagc atactccctt ttagaaaaag caaaaaaaaa
     6661 agaaaaaatc ttattcttga taacaagcta cgtgacgtat atgcctaaca aaaatagaac
     6721 ttttgaatca atcttaaatg aaggtttaac cacagaaccg gcatccatta tttttgatga
     6781 agttatcgat gacgttgaag atagtaagta tcataaaaat ttaaaagaac ttttttattt
     6841 tgcactcaac aatggaccaa gtttgttcac tggtatatca gtacctgata gtgataatcc
     6901 attaattatg gcaaaaattt acttaacaaa atggtgtaat aaatacatca gggataggaa
     6961 taacccagca ttaaaaaaac ctcttaaaac atttggcgaa aaagatgctg ctttaactac
     7021 tagagtagca gcaaatgcta atattgatga tcaaaaagta ttgaatgact acttgcgtgg
     7081 acatttttta tatatgagcg ctgagaacat gaacggaagt attttagagg aatatttagc
     7141 cgaagtgtta gaacctgaag gttggatttg gtgcgccggc tctgtatatc gagctgttga
     7201 tttctgttat ttaggaacct caccaattct tttacaagtg aaaaataaat acaatacaga
     7261 aagcagttcg agttcagcta taagagtagg aacaacaatt agaaaatgga atcgtttaaa
     7321 taagtcgaca aaaatttctg gtttggatag ccctatcccc aattggaaag cattaataga
     7381 aatgactgaa gctagtaagg aactggcagc taagttaact gaaaatagtt atttagctta
     7441 tattaatgaa aaatccacac gagaattatg gacactcgat gactaacaat atccttatta
     7501 gtatctatcc acgatacatg gaaaaaatta taaacaaaca aaaaaacttt gaatttagaa
     7561 atttctatct tacctcagat ccttataata tcttttggat ttacgaaaca atccccacca
     7621 aagctttaaa gtataaattg attgtaaaaa atccaattac taaacttaag aaaaatcaac
     7681 actacttttt aggagacgat gagtttgaac aattagttta aaag
//
LOCUS       DAXWOO010000134         7693 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.136, whole genome shotgun sequence.
ACCESSION   DAXWOO010000134 DAXWOO010000000
VERSION     DAXWOO010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7693
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.136"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     regulatory      80..246
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            394..951
                     /locus_tag="ACGE70_002231"
                     /pseudo
     CDS             394..951
                     /locus_tag="ACGE70_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            1009..3048
                     /locus_tag="ACGE70_002232"
     CDS             1009..3048
                     /locus_tag="ACGE70_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFF8693844.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLYAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(3157..4308)
                     /locus_tag="ACGE70_002233"
     CDS             complement(3157..4308)
                     /locus_tag="ACGE70_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFF8693845.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4462..4992)
                     /locus_tag="ACGE70_002234"
     CDS             complement(4462..4992)
                     /locus_tag="ACGE70_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFF8693846.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(5091..5441)
                     /locus_tag="ACGE70_002235"
     CDS             complement(5091..5441)
                     /locus_tag="ACGE70_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFF8693847.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5598..7127)
                     /gene="ahpF"
                     /locus_tag="ACGE70_002236"
     CDS             complement(5598..7127)
                     /gene="ahpF"
                     /locus_tag="ACGE70_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFF8693848.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(7137..7691)
                     /gene="ahpC"
                     /locus_tag="ACGE70_002237"
     CDS             complement(7137..7691)
                     /gene="ahpC"
                     /locus_tag="ACGE70_002237"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFF8693849.1"
                     /translation="MGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFTFVC
                     PTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSHQIS
                     RIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRTHPG
                     EVCPAKWKESGETLKPSFDLVGKI"
BASE COUNT         2626 a         1487 c         1410 g         2170 t
ORIGIN      
        1 taaatttcac tatttcattt caaattccat tgcctaatca ttaggaaaag tctatcatga
       61 actcgtaaat agaatatagt aataaccttg ttaggtgagg ctcctataca aacataggct
      121 gttgcccaga aacgtcgaaa gtcgccaatg ggtaaaacag aaactgttgg attaaggctt
      181 tttccaatag ctaagaatgt tttctttacg ttgtatagtg ctaaaactcg gacgatgaga
      241 tgatgctagg tcagaattct tgttcgaaaa ggtttctgac cttttcttta tatttttgtg
      301 actgacttat ttgatgagac aaactcgctc ctcacctgac aagtggattg ctgtgtgagg
      361 aggagatgcg gtgaaaaaag atcgattggt ggaggtcgct cggattcact tctggttgca
      421 caatacaacc agaaaacaca acaagcaaaa ctgatctcta tcatgagaga ttcatatgtc
      481 gatattccag gttacggaat gaataagata aatgcagctt actcttatgg gggagttgat
      541 ttattaaatc aaacattaaa ggaaaatttc aaatttgaag ctccctatta tgcaagtatc
      601 acatttcaag attttatcga ttgcgttaat gaactgtttc ctggtggagt caagatcgat
      661 gcagaaaaat ctctagattt agatggcgta tatataaaca aaggagagca agtgatggat
      721 ggtaatactt tactccagta tgctcgattt cgggaagaca aagaaggaga cttcgggagg
      781 atcagaaggc agcagcaagt gatcaaagct atctctcagc agctgaaaga cgtaacttca
      841 atattcaagc tacccaaagc ggttgggaaa ttacttggta gtatccaaac gaaccttcca
      901 gaaagtgtgc ttctggattg tggtatggat ttcctcaaag acatatgtta gaataaacag
      961 gtataaatag tgaaataaag gaatgacaag caagagaagg aggaaaaaat gaaaagaagt
     1021 gacaagcacg gcaaaaatcg aacaggcgct tatattgccg gcgcagtgat cttaatagca
     1081 gctgcgggtg gcggttattt ttactaccag cactatcaag aaacccaagc agtagaagct
     1141 ggagaaaaga cagttgagca atttgtccaa gctttgaaca agggagatta taacaaagct
     1201 gcagaaatga catcaaaaaa ggcggcaaat aaaagtgcat tatctgaaaa agagatctta
     1261 gataaatacc agaatatata cggtgctgct gatgtcaaag gacttcagat atcgaatcta
     1321 aaagtagata aaaaagacga ttctacttac agtttttcat ataaagcaaa gatgaatacc
     1381 tcattaggtg aattgaaaga tctttcttat aaaggaacat tagacagaaa tgatggtcaa
     1441 accacgatca actggcaacc caacttggtt tttccagaaa tggaaggaaa tgacaaagta
     1501 agcctgacca cacaagaagc agcaagaggg aatattatag atcgaaatgg tgaaccatta
     1561 gcaacaacag gcaaactaaa acaattagga gtcgttccaa gtaaacttgg agatggaggc
     1621 gaaaaaacag ccaatatcaa agcgattgct tcctccttcg acttaacaga agatgctatc
     1681 aatcaggcga tttcacaaag ctgggtacaa cccgattact ttgtcccatt gaaaatcatt
     1741 gatggagcaa cgccagaact tccagctgga gctaccattc aagaagtaga cggcagatat
     1801 tatcctttgg gtgaagcagc tgctcagctg attggttacg tgggagatat cacagcagaa
     1861 gatattgata agaatccaga attaagcagt aatggaaaaa tcggtcgctc aggtttggag
     1921 atggcttttg ataaggatct tcgtgggaca acaggtggga aattaagcat tacagatgca
     1981 gacggtgtag agaaaaaagt tctgatcgaa catgaagttc aaaatggcaa agatatcaaa
     2041 ttgacaatcg atgcgaaggc acaaaaaata gcttttgaca gtctaggagg aaaagctgca
     2101 tcaactgttg cgacaacgcc aaaaaccggt gatcttcttg cgcttgctag ctctccaagc
     2161 tatgatccaa acaaaatgac aaacgggatc tcacaagaag attacaaagc ttatgaagaa
     2221 aatcctgaac atccattcat cagccgattt gcgacaggtt atgctcctgg atctacgttt
     2281 aaaatgatta cagcagcaat cggtctcgac aacggcacta tcgatccaaa tgaagtgttg
     2341 acgatcaacg ggcttaaatg gcaaaaagac agttcttggg gatcttttaa aacaacgcgt
     2401 gtcagtagtg atgtatcaca agtagactta aaaactgctt tgatctattc cgataatata
     2461 tatgcggcac aagaaacgtt gaaaatgggt gagaaaaaat ttcgtacagg cttagataaa
     2521 ttcatttttg gtgaagacct tgatttgcca attagtatga atccagcaca aatttctaat
     2581 gaagatagct ttaactcaga tatcttgcta gctgatactg gatatggaca gggcgaactt
     2641 ctaattactc ctatccagca agcagcaatg tattctgttt ttaccaacaa tggcacactt
     2701 gtctatccta aattgattgc agataaagag acaaaagata agaagaatgt catcagcgaa
     2761 acagcagtac aaacgatcgt gccagatctg agagaagttg tgcaagatgt aaatggtaca
     2821 gcacattctc tttctgcttt agggattccg ttggcagcga aaactggtac agcggaaatc
     2881 aaagaaaaac aggatgtaaa ggggcaagag aacagtttct tgtatgcttt caatcttgat
     2941 aaccaaggtt atatgatggt cagcatgttg gaaaataaag aagatgatga ttcagcaacc
     3001 aaacgagcat cggaactatt gcaatacctc aaccaaaatt atcaataaaa aattgctttt
     3061 taagtacagt aaaaggactg tggcgttatc gtcacagtcc ttttttcaaa taaacggttt
     3121 tcacgatgtc tctttcacaa cttctttttg atttactcac acttcataaa ctttttttgt
     3181 aaaatatttg agaacaagtg gagaaataag tgtacttaat aaaatcacga taactaaagg
     3241 tgaatagtac tgtggttcga tcaattgact ttgctgtcca atttgtaaaa tgatcaaagc
     3301 catttctcca cgcgaaatca taccagcacc gaccatcaat gaactatgtt gagaaaaacc
     3361 ggcaatctga gcaccaaaat aacctccaag caatttggtc aaaatcgcta caagcgtcaa
     3421 tatagaaata aagagtagtt gttcactaaa gtcagaaaaa tcaacttcta atcctacact
     3481 gacaaaaaat actggtataa atactgcata tcctaaagcc tctacattgt ggtatacttc
     3541 ttctttaacc tttgtctgac tgacagctat cccagcaaaa aaagcaccaa tgacagagct
     3601 aagcccaaca agatccgcta cgtaagacat tcctaaacaa ataactagcg atacgatgat
     3661 aatagaagaa tttgcgtaca ctttttctgc aaatgaaagt aggaaaggcg caatccattt
     3721 gactaataag aaaataaaaa caaaataaat tagctgttca atcaaaacaa gcggtaaaga
     3781 agcattgcta gaagcttccc ccgttagaaa agataggctg aaacttagaa ctaaaacgac
     3841 taaaatgtca tctaccacag aagcacccaa aattgttgat ccttcttttg tattcactac
     3901 gtttagttct tttaatactt ctactgaaat actgacagaa gttgccgcaa gaataatccc
     3961 aaagaaaatc gcttcatttc ccgaaacttg gaatgctttg cctgtcaacc agccaaaaac
     4021 aaccgggaag atgatcccaa gcaaagcaac gaacatacca ggtcggaaat attttttcaa
     4081 aagagataaa tcactttcca gaccagctaa aaacattaat aagatcacac caatctccga
     4141 aaagtcatgg actagaatat caggtttgac ccaattcaat ccagcttggc ctagcaaaac
     4201 accaacaagt aattgtccaa tcactgctgg gatacctaat ctccgcgaga tatgtgagcc
     4261 aatcgttgtt gaaatcaata tcaggcataa aataccaata aattccattc tcatcattcc
     4321 tttgtataaa atatagcccg aacttagcat aacatacatt attgaaaaaa taggaaaata
     4381 tactcacaaa aaaaccaaca atccaaaaga tttttttgga ttgttggttt ttatacggtc
     4441 tattttaata actaaaaatg attaacgtat agtcattttt cttattctga tgaaattgat
     4501 aatctcctgg atgcccatga aaatcaaaat aaagccgaat atttggaata gaagcatcaa
     4561 acttttgaat ggattgaaca ataagacgaa tccgccaatc aacaacagaa tactactata
     4621 aacactccaa cttttagctt ttgtattcat gctgaagaac aactggaaca agctgttgat
     4681 gataacagct aatccaagaa gaatcggcag gatagaaacg attgcaggtg caaaagcata
     4741 gacgatcaga gctaagatca ggaaaaaaag accactgaac agaccgatac tccaagaacc
     4801 tgttttcctt ttaaaacggt aagcctcaaa aagattcaac aacccaagca aaacaagata
     4861 ggcggtgatc agataaccga taaaatgaaa aacagcagaa gggtttagca caatcgcaat
     4921 cccagaaata atataaacag cagctcttaa taaggcgtat ttttgaatat tttgtttaat
     4981 cgtctcaaac atataagaaa acctcctttg acttatttta acgtgaacta gtcattttac
     5041 caaattaaat gttacaaaat gagcgaagga gaagaatatc tctggaaaaa tcaacttctg
     5101 aggatctttt ccattttttt gccttttgct agctcatcga tcattttatc taaataacga
     5161 atttctttca tcaagttatc ctcgatgtct tctactcgta caccacaaac aacacctgta
     5221 atcaaggaac gtgaaggatt ggggacaggg gcttccgcaa aaaattgttc gaaagtcact
     5281 tgtttttgta gttgatattc tagttctgtt tgggaataac ccgtcagcca acaaatgatt
     5341 tcatctactt cctcttttat acgatctttt ctctccgctt ttgctacata cataggataa
     5401 acgcttgcaa aacttgtcgt aaaaatacga ttttgcgcca taatcagtca cctttctttt
     5461 tttcaaatta tttactactt atagtatata agaaaaagag aagaaaaagc gattattcaa
     5521 aaaaacaaaa aggattgtga caaaattctt gtcaccatcc ttttttccgc tctctgactt
     5581 tttacaatct tcatagctca agtgcgcatc agataatcga atgctcctaa agctgcagta
     5641 gctcctgaac ccattgaaac aatgatttgt ttgtatggac tatctgtaca atcaccagct
     5701 gcgaaaacgc cggggatatt cgttgcgcca tttttctcgg tcactatttc atttcttgga
     5761 ttcatttcga tcgttccatc tagccaatcc gtattaggga gtaagccgac tagaatgaat
     5821 actcctgaaa cagctagtgt atgcatctcc atcgtacggc gatcgatata ggagagagat
     5881 tctacctgat tcgtaccgct gatttctttc gtcgctgcat gtttgataac agtgacattc
     5941 gcaagagaga ctagtttatc ctgtaagatt tgatctgctt ttaattctgg taagaattct
     6001 agaacatata catgttttgc taacccagcc aaatcgattg ctgcttcaac accagaatta
     6061 ccaccaccga taacagcaat ctcttttcct ttaaataaag ggccgtcaca gtgtgggcaa
     6121 taagcaattc ctttattttt aaactctttt tcgcctggta cattgataga acgccagtgc
     6181 gcacctgtcg cgatgatggc ggtttttgct ttaagtgata taccgttagc taagccaata
     6241 ttaataaaat gttgctcttt atttaaggaa acggcctgtt gattcatcat aatgtcgata
     6301 gggtaactac gcacatgact ttctacttgc tgcatcaatt ttggaccttc tgtgtaagag
     6361 gtaccaatca tattttcaat ccctagtgtt tcaacgactt gtccgccgaa agaatcagcg
     6421 acaagtcctg tacggattcc tttacgtgct gcatagatag ctgcactact agctgctggt
     6481 ccaccgccaa tcactaatac atcataaggt tctttttcaa acaactcttc ttcaatcaaa
     6541 gggccagtga ttttttcaac aatttgttcg atcgtcatgc gtccgctact aaattcttct
     6601 ccatttaaaa acacagttgg cactgccatg atttttttat catcgatttc tttttggaac
     6661 atcccacctt caatcatcgt atgagtaata tttggattta atacagctaa tatattcaat
     6721 gcctgtacaa cgtcggggca attatgacaa gtcaagctaa cataactgtc aaaatgcaat
     6781 gtttgtgtta tttttttgat tttaagactc aaagattcac tgattttcgg tgcccgtcca
     6841 ctaatttgta acatagccag taaaaaagag gaaaattcat gaccgagggg gataccagca
     6901 aaaacgattc cactttcttt gccaactgga ttgatagaaa aactaggtgt gcgcatcgaa
     6961 tctctttttt cccaagagat tttaggagac atttctgcta tttccttcgt aaattcagct
     7021 agtttttgac tattttcact attatccaaa ctaacagaaa aaacaatagg agattcaatc
     7081 agtgctaaat attgttcgag attttgttta gtttctttgt ctaacatatc caccacctaa
     7141 atctttccaa caagatcaaa acttggtttc aatgtttcgc cactttcttt ccattttgcc
     7201 ggacaaactt cacctgggtg ggtacgtaca tattgagcag cgcgaatctt atcaatcagt
     7261 gcactagcgt cacgaccgat tccatctgca ttgatttcca tagcttgaac gattccgtct
     7321 ggatcaataa taaatgttcc tcgctgagct aatccttgct cttcatccaa aacatcaaaa
     7381 atacgagaaa tctgatgaga cggatcaccg atcatcacgt attcgatttt accgatagca
     7441 tctgatgtat catgccatgc tttgtgagta aagtgggtat cggtagagca agaaaagact
     7501 tcaacaccta aagatttcaa tgtactgtat tgttcttgta agtcttctaa ttcagtagga
     7561 caaacaaaag taaaatcagc tggatagaag caaagaatac tccagttccc cataagatct
     7621 ttttcactga cttctaaaaa ttctccttga tgataagcat gtgcattaaa ttcttcgatt
     7681 tttttcccaa tca
//
LOCUS       DAXWOO010000135         7600 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.137, whole genome shotgun sequence.
ACCESSION   DAXWOO010000135 DAXWOO010000000
VERSION     DAXWOO010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.137"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(138..428)
                     /locus_tag="ACGE70_002238"
     CDS             complement(138..428)
                     /locus_tag="ACGE70_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693850.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(546..806)
                     /locus_tag="ACGE70_002239"
     CDS             complement(546..806)
                     /locus_tag="ACGE70_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693851.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(943..1356)
                     /locus_tag="ACGE70_002240"
     CDS             complement(943..1356)
                     /locus_tag="ACGE70_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFF8693852.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1812..2297)
                     /locus_tag="ACGE70_002241"
     CDS             complement(1812..2297)
                     /locus_tag="ACGE70_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693853.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2436..2651)
                     /locus_tag="ACGE70_002242"
     CDS             complement(2436..2651)
                     /locus_tag="ACGE70_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFF8693854.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2875..3675
                     /locus_tag="ACGE70_002243"
     CDS             2875..3675
                     /locus_tag="ACGE70_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFF8693855.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3694..5562
                     /locus_tag="ACGE70_002244"
     CDS             3694..5562
                     /locus_tag="ACGE70_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFF8693856.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5555..6046
                     /locus_tag="ACGE70_002245"
     CDS             5555..6046
                     /locus_tag="ACGE70_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFF8693857.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6034..6588
                     /locus_tag="ACGE70_002246"
     CDS             6034..6588
                     /locus_tag="ACGE70_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFF8693858.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6606..>7600
                     /locus_tag="ACGE70_002247"
     CDS             6606..>7600
                     /locus_tag="ACGE70_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFF8693859.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2707 a         1213 c         1443 g         2237 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctttgt gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag
     1501 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
     1561 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
     1621 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
     1681 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
     1741 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
     1801 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
     1861 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
     1921 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
     1981 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
     2041 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
     2101 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
     2161 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
     2221 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
     2281 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac
     2341 ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc
     2401 atgaagataa taactttttt aataattata gctttctaat atctttcatc aacttttcta
     2461 gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg
     2521 atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac
     2581 aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct
     2641 aaagttttca ttagaattct cctttcttaa atgataaaac aattatatat caaaaagaga
     2701 aacattgctt atataacgct caggaatttt agcaatctat gcaaaaagat gaaaattttt
     2761 tttgctcttc cattagtaca cattttttag ctgaagctgt aaatttcatg tcttaatttt
     2821 ttttagtgag cttttatact ggatttgtaa gaaaaaagaa ggagaggatc acatatgtcg
     2881 gattggttgg atattgcagg aaaagtagta attgtaactg gaggttcctc aggaattgga
     2941 agaagtatcg tcgaaaattt gttgaagcag aatgcacaag tagcgaattt tgatgtaact
     3001 gaatgccgta tacagcatga aaacctacta tctttaaaag tcgatgtgtc ttctaaaacg
     3061 gacatagaag aagggatata taaagtaatg aaacatttca agacgatcga tggtctagtc
     3121 aataatgcag gaataaatat tccttctctt ctaatagata gaaatcatcc aaaaagcaag
     3181 tatgaattat cagaacaagt ttttgataag atgatcgctg tcaatcaaaa aagtgtatat
     3241 ttgatgagcc aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt gatcgtgaat
     3301 ctttcttctg aatcaggatt ggaaggatca gaaggacaga gctgttatgc tgctacaaaa
     3361 gcagcgatga acagttttac tcgttcttgg gcaaaagagt taggaaagag gaatgttcgt
     3421 gtggtaggtg ttgcaccagg gatactggaa gaaaccggtt tgagaacaca agaatatgaa
     3481 gaagctcttt cttatacgag aggtataagc gtcgaacagc tgagaaacgg ttacagtcga
     3541 acaagtacga tccctctagg aaggagtggt aagctgcaag aagtggcgga tctagtctgc
     3601 tattatcttt cagaaagatc aagttatatc actggagtca cgactaatat ttcaggaggt
     3661 aaaacaagag gataaaggat aggggggacg cacgtgacgc tagtaaacag atggtaccaa
     3721 ttattgaaat tactagtcac tcacaagaaa gtttcaatcc aggaagtgca aaaagaaatc
     3781 aaaaccagta atcaaaccat aaaaaaagat attggggagt tgaatgaaga aattgccggt
     3841 attgcaaaaa tcatccaaaa aaacaaacat tttgaacttg aaatttatga ttttgatttt
     3901 tttgatgaga tcatgcaagg aaaattgaaa acagccagtg atttcaattc ggcatccaaa
     3961 agaatcgctt atttactaaa taggctgatc gaaacagagg atttcttacg tatggatgat
     4021 ttgtctgaag aagtcggtgt cagcagagga acagtggtca aggatctaaa gacattaaaa
     4081 gagatgatca atgattttga tgtaaagatt acaggcacac caaataaagg actaagaata
     4141 ataggagatg agctagaatt acgactactt ttattttatt ttgtttctcc ctacttttca
     4201 gagacgaaaa cagaacagtt tctagaagaa aaacaagcaa tcaaacattt tcagaagaag
     4261 acgaatgcta gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat ttctttgaat
     4321 agaatctcta gtaactatct gttgaagaag cccatcaatc gttacagtgg gtgttttgaa
     4381 tatatccaag aattcaatga actgatcaat cagttggaag aaatctatga actaactttg
     4441 agccaatatg aaaaagattt tatcagtttt cctttgaaca tcatcaacac aagtataacg
     4501 atgataagga aagctaactc tgatttgcgc ttatatttcg accccatgat gatgcgtatt
     4561 cgacgattag atctcattga tctggatgag tccttcttat atcaagagat gaaacagcac
     4621 ctctctttat tgataaatcg ggtaattttt cgttatcgat tgactgattt attttatggt
     4681 gagatagaaa aaaagtatcc tttttcttac caaatcgcta gcgaatgcat acaagcatta
     4741 gaagctatct tgcaacataa gatctctaaa gtagagatca gctacctaac gttgtatttg
     4801 gagttagcat ttcgatatca aaaagagcat ttttcctcta aaaaagtagc cattgttcat
     4861 acgacaggaa aaggaacagc attaatgatc caaagacaaa tcaaaaggat attggggagg
     4921 gatatacaaa taacacatgt ttcagaagaa caatatgaaa aagtagatct aaacaaatat
     4981 ttcgctgttt ttacaacgat tccgttaaaa aatgtaaatg aagagacacc ggtgatccat
     5041 ttgactaatc tttttgatga ctcttggtta agggcacaat ggcaaaaagc aaaaaaaaag
     5101 cgattagccg ggtttcattc tactgttttt ttctatcaaa tatggaaaga aaaaaagacg
     5161 tatcaagaat atttaaacaa attagtcgct cctttgatca gaggctatta tgtggatgaa
     5221 gaatttgaaa aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt taacaaagaa
     5281 tttgcttttc ctcacacaac caaccagcta tctcaacaca ttgttttatc agtagggcta
     5341 ataactgatc aaggtttagc agaaacagga ggaaatggag ttattttttt atttgccata
     5401 cctgaagaaa tgacggctga agttgagaat gaattatttg agctatacga tatcctattt
     5461 gaactcataa aaatgggaat agatgagttt attgaattag caccgacaca agaatcattt
     5521 ttaaaactaa tcaaaagtaa aggagagaga cttcatgaat gatatgtatc tattcatagt
     5581 tttcgcagct tcagcttatg tgctgcaagc agttatcagt tggaagcaac taaagcattt
     5641 caatcaaaca tatagcgaac tgcgaagaaa aggaaaagta gcgattggta aacgacctgg
     5701 gaaaatccgt tcaggcacga ttgttatgct agctatagac gatcatgatc aagttttaga
     5761 agtcagaaaa atgcaagggg ttacatcttt ggcaaaattt cattcattac ctgtctatgc
     5821 gggtgaagat atccattaca tcgatcgtta ccatccctca gttcgagcgg aagatcgttt
     5881 gacacaagca gctttagaaa atgccagaga agtatactta cgaatagagg caggaaatga
     5941 aaaaagtgaa caaccttcat tgccttttat tgctatgctc aataattttc ggatatggaa
     6001 aagtacgatc aaagaaaaaa ggagtgacaa acgatggata tcataacaaa atttgctgag
     6061 ggatttatga ggctgttcca aacaggtgca gaaacattca taagctggat gacaggaatc
     6121 gtaccagttg tcttgatgct gatggttgca atgaatacgc tgattgcttt tttaggtgaa
     6181 gaccgagtaa cgaaagtagc caaattatca gcgaaaaatc cgcttactcg ttaccttgtg
     6241 ttaccgttct tatctgcttt tatgttggga aatcctatgt catttacaat ggctcgtttc
     6301 cttccagaat actacaaacc aagctattat gctgctcaag ctcaattttg tcatacgagc
     6361 aatggtgtat tcccccatat taatcctgga gaattgtttg tatggatggg gattgctcaa
     6421 gggatcgaaa ccttagggct aaattcgatg gagctggcta tccgatactt gcttgtggga
     6481 ctgctgatga actttattgg tggttggata actgatttta ctactggatt tgtatgcaga
     6541 cagcaaggaa tcgttcttag caaaaaagta gagctttcag ttgattagag aaaggagagt
     6601 tttaaatgta tggtgatatt aagatcgtaa gaggaaacgg cgggtatgga ggtcctttga
     6661 ttattaggcc gacaaaagat aaacacaagt tcatctatgt cacaggtgga ggtgagaggc
     6721 caatgatcgt agataaaata gaagaactga caggtatgga gtctatcaat gggttcaaaa
     6781 catcaatacc tgatagtgaa atcgcgcttg caatcgttga ctgtggaggg actcttagat
     6841 gtggaattta tcctaaaaaa gggataccaa ccatcaatat cgtttccaca ggaaaaagtg
     6901 gcccattagc acaatatatc actgaagata tttatgtttc agcagtaggg ttagaacaaa
     6961 tacagccact aatggaaact gatgataaca aagaaacagt aaatactgca tctccagata
     7021 aaaagtacaa atatgatact tctaaaaaga taaccgagca aaaagcagag cagcaaaact
     7081 ggatagcaaa aattggaatg ggcgcgggaa aagtagttgc tacattcaat caatcagcaa
     7141 gagaagcagt agatacgatg atccggacag tgatcccttt tatggctttc gtttcccttt
     7201 tgattggtgt gatacaaggt tctgggatcg gtaatgtatt tgctaacatt atggcacctt
     7261 tagcaggaaa tgcagttgga ttaacaatca ttggctttat ttgttcactg ccatttttat
     7321 ctcctttgct tggtccaggg gcagttatta gccaagtgat tggaacattg atcggtgtag
     7381 agattgggaa aggaaatatt cctccccaat tagcattacc ggcactattt gctatcaata
     7441 ctcaaaatgc atgtgatttt attccagtcg gtctaggttt ggaagaagca aaagctgaga
     7501 cagtagaagt aggtgtacca tcagttcttt attcaagatt tcttaacggc atcccacgag
     7561 ttatcattgc ttgggtagca agttttggat tatatagctg
//
LOCUS       DAXWOO010000136         7464 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.139, whole genome shotgun sequence.
ACCESSION   DAXWOO010000136 DAXWOO010000000
VERSION     DAXWOO010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.139"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            313..876
                     /locus_tag="ACGE70_002248"
     CDS             313..876
                     /locus_tag="ACGE70_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFF8693860.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
     gene            complement(1155..1442)
                     /locus_tag="ACGE70_002249"
     CDS             complement(1155..1442)
                     /locus_tag="ACGE70_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFF8693861.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPNLLCGTHENFYEQLKKIR"
     gene            complement(1432..1761)
                     /locus_tag="ACGE70_002250"
     CDS             complement(1432..1761)
                     /locus_tag="ACGE70_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFF8693862.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            2066..2395
                     /locus_tag="ACGE70_002251"
     CDS             2066..2395
                     /locus_tag="ACGE70_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HFF8693863.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            complement(2682..3167)
                     /gene="vanZ-A"
                     /locus_tag="ACGE70_002252"
     CDS             complement(2682..3167)
                     /gene="vanZ-A"
                     /locus_tag="ACGE70_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFF8693864.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(3320..4231)
                     /gene="vanY"
                     /locus_tag="ACGE70_002253"
     CDS             complement(3320..4231)
                     /gene="vanY"
                     /locus_tag="ACGE70_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HFF8693865.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYELWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            complement(4659..5267)
                     /locus_tag="ACGE70_002254"
     CDS             complement(4659..5267)
                     /locus_tag="ACGE70_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFF8693866.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            complement(5273..6304)
                     /gene="vanA"
                     /locus_tag="ACGE70_002255"
     CDS             complement(5273..6304)
                     /gene="vanA"
                     /locus_tag="ACGE70_002255"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFF8693867.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            complement(6297..7265)
                     /locus_tag="ACGE70_002256"
     CDS             complement(6297..7265)
                     /locus_tag="ACGE70_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFF8693868.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
BASE COUNT         2272 a         1495 c         1258 g         2439 t
ORIGIN      
        1 aattcaaata ttgcatgtta tatctaatat gggagcattt tataattttc attgtgataa
       61 actcgttatt aactttagga tacactagtt aggattaaat taggtatcta gaaatttact
      121 taagattttt ttgtttattt cgtatacaca ctcgtatcaa aaacaatcga ttatgagaca
      181 aaatgaaatg acctctttta ttaaataaaa gaggtcatat tttgtatcat aacttgttgc
      241 attaactatg tatctataac tcccttttat gatacaatca agccagaatt aaagtacgaa
      301 aggaagaaaa gcatgagtgg atacatttat ggttatgccc gagtgagtac acgttcacaa
      361 gaactaaatc gtcaattaga cttactcaat gaacaaaatt gtaatgaaat cctaactgag
      421 aaaatgacgg gaaccaaagc ggttcgtccc caactaaatc gtttaaaaga taaaattcgt
      481 ccgggagata cggtaattgt agaaagtttt tctcgattgg gtcgtagtac aaaggatttg
      541 attgaccttg ttacctattt tgaagaacac catgttaagc tggtgagctt gaaagaaaac
      601 ttcgatacca gcacgcctca cggacgctta atgatgactg tcttccaagc atttagtcag
      661 ttcgaacgtg atttaattgt tgaacgtacc aaagaaggtt tgaaaagtgc acgtgcgaga
      721 ggtcgtaaag gtggccgtcc acgagtgaac cagaaagatg ttgatcgagc agtaaaattg
      781 tataaaagtc aggtttatag cgtaaaagaa attacggaaa tgactggtat tagcaaggca
      841 acactctacc gctacttaaa agacaatagg gaataatcta ttttaatctt taggaatcta
      901 taatatttat gggagaatta gagtgatttt tttattatta ctaaaaaaca atataaaata
      961 acgcatttta agccctcttt gtgcataagg atgaagcttt taatttgaaa gaggcttaaa
     1021 atgcgtttta attttttcta tttttcataa atatataggt tagtggttta aaattatagg
     1081 gtattacaac tctcagctgt taaaaattat cttttaaagt gtcgtctatc ctctcaagtt
     1141 gaaacattgg gttcttacct aatctttttt aattgttcat aaaaattttc atgcgttcca
     1201 catagtaagt taggtatcac tgcattatct ttaatttcat atgcaacgcg atatgtcgta
     1261 cctgcatact tgaatccaat agcccaaatt cctgataaat cacccgtttt ttgttctccc
     1321 gaatgaggac gaactgctat ctcatcataa attatcgtta aaaattgttc ttttaatgtt
     1381 ttctctttta atttttttag atactttatt acgtgtttag aaggtagaat ctcataagcc
     1441 aatttctgct gccaactcct cttttgtcat cggtttagaa tttgcaacag tatcttcagc
     1501 aatagattta aatgcagaag ttaacgcatt ttttctattt ctaaattctt ttaagatctc
     1561 ttgtttgcta taaccttctg aaagaatatc agccaaaata tcttcactaa aatcaaaaaa
     1621 ttctttttta ggctctacaa actcataaag gataccattc tctacaagct tagcttctac
     1681 ttcaacacca ttgggcacat cgaattcttt agggaccgtt aacataatag aatttccttg
     1741 ttttcttgtt tttgtaatca tattatttcc tccattattt tacatttcgt aattacttag
     1801 taattacatt gtacgctatt tttagtagaa atacaaaata ataaaactcc tttagaacaa
     1861 ttttcaaagg agttttattt gattaggaaa ttatgtagaa taataatatg tttttaactt
     1921 ggaatcaaaa acgcacttgc aagtaagttt acttcaatac atgaaattgc ttataatagt
     1981 gtattggaat taaggtagat tgacaaaaga gtgatcaaat aggtatactt cattcaaata
     2041 atcgtttgaa tgtgaggtga gatgaatgaa aaaagtttgc caagtgactg tcgtacataa
     2101 agaaaaagta aatggaataa aaaataaatt ggatcaaaaa gatttttcaa acttacttgt
     2161 gttagggaaa tgtttcagcg atttttctag aattaaaatt ttctatgctt tagaaaccta
     2221 taaggaaatg tgcgtctgtg atttagcaga aatacttact gctagtgtcg ctacaacgtc
     2281 acaccattta cgttttttaa aaaaacatgg aatggcaaaa tcacgtcaag atggaaaagt
     2341 agtttattac tctttagcga atgaagacat cctttccgct attcgtgttt tttaaagtta
     2401 tcagaaaatc tatctatgaa atcttagttt gggggtaccg tcaggaaaat gcggatttac
     2461 aacgttaagg aaatttatta aagagaccaa ttcctcagca ctaaaattga taattgggct
     2521 ttctttgtta ctatagtgtt aggtaagctt tttactttat caccggtttt cccctcactt
     2581 cacacctacg ggcgagtttc cccacatccc gttttccatc taatccaatt cattcaatat
     2641 ctttatgtgt atgaaagatc agattgcttg atttagacat cttacacgta atttattctt
     2701 aaatgggtac ggtaaacgag caataatacg agcaaaagta tacctacaaa aataataact
     2761 ctgtctaatt ttttttgatt catatgctta ttgcttaaac catataattt cagtccaaga
     2821 aagcctccaa cagtatttgt aattacatct gttatgtctg tcgctccaat agcgaagata
     2881 aattgaatta tttcaaaagt aagacttaaa accagtacaa aagcaaactt aggtaaaaat
     2941 ccgatttctt taaaattgac attcaaaagc aagccaaatg gaataaagat tataacatta
     3001 tctatcatct ctctgaaatt cccagtagca gtaaatggag tcaagttaag acttctttga
     3061 tgataattaa atactgataa aatattgtat tgtaatttga ataacactaa ccagattagt
     3121 gtcactaaat ataaagctag caatcctcta gataatattt ttcccaatat aatttcctct
     3181 aattcttttg tttaagttgt atcattagta tagcaatgtc ctagttaacg tgggttttaa
     3241 attaaaaagc taacgttacc ccaaaagtgc tagtacaata atgctgttca ttaatttcgt
     3301 tggtttcatt ccgccatcct tacctccttg aattagtatg tgttgatccg ggaaacactg
     3361 tcacaattac accgtctata ttgtttcctg atatctcata acgaagatta gtcggcacat
     3421 gaatggtggt atttttagta acaggataat aaaagatctc atatttttcc ccatttacac
     3481 taacagaaat ggttttttct tcttttaggt aatccatata ttcctcgaga acgaaattct
     3541 tttctttcat aatcgcacta tgtggtaaac caacatagcg aatatgccat agttcatatt
     3601 gaattcctgt taactctgtt ttgtcctctg gataacgtaa aatgaacccg tatttccaag
     3661 cattttcttc tatccacttt ccttcagggg ctcgttccat tttcgtcaag cttgatccta
     3721 catctagtga taaacctgaa ttatgctcac tataacctgc tggtaaggca tactcagccc
     3781 ccatttcttg gtaaagcaca ctttgctcat caaagtctcg atagccacta ttaataataa
     3841 aatgactaac gccacccttt acagcatcat tgaccatctc tgaaaatttt tgtgctattt
     3901 cttttgacat ataaatatta ctatcaagca acccgtatcc atttattaat tcgtcatgtt
     3961 tagataaatt cacgatatct gacttcacac tttcttggcg aacaggatat ttactattga
     4021 ttaatagcag atttccttga taaacctgtt cttctgtaat tgttttctct tgggtatttt
     4081 cagaagtccc actattttct aaatgttctt tgggattttg atcataattt tgaaattcga
     4141 ctttttcctg agaaaacagt gcttcattaa cgtagtcata acctaagtat attaagaata
     4201 acaataacaa taaaaaaaac aacttcttca ttttcagtct cctccttaac tatatagttg
     4261 gaagtatgaa atatttctag aatcttcccc ttaaacggca caatctcgtt tgagattgtg
     4321 ccgttttgcc ccgtaaatta cagtcaatta tttcgcccta ctggatttac tattatcacc
     4381 aatgtagatg aattttcata agtgataccc atgctgaatt taacgaggct gaactgcctg
     4441 ttgacaatgg gcggtgtgaa ataccacccc tctatgcgat cagtcttatt atcaggctat
     4501 cttgccgcag cgcacgcaga taatgaccgc aatatcgctc tgccttacat cttcaatact
     4561 atctactata tataaattcc ctaagaacca gttacatacg tcgggtttcc tgccgaaaga
     4621 gttagcttat atagtttgtc cgtgcaacgg ttaaaagttt atttaacggg gaaatcaaaa
     4681 tagctattgg ggtatggttc gtctcttaat acatagtgcc accattcgag gctatatgct
     4741 tcaaacccac tgttttccat gatggagcgc aaacgtctgc gattttgcgc ttcattgcat
     4801 gatattccat ttgccgcatg atgagagcgt tcatccataa aatcaaatcg gctccccatt
     4861 ggtacaagct cacccgtgtc taatcgataa agcgtaagat caatggcact gccgcggcta
     4921 tggcttgatt ttgaagccac gtatcctttt gaaatcatct cagttcggtc aatattggga
     4981 taataacttt cctttgtcag gttattttcc ggctgtgcag cccattgcat aaaacagttt
     5041 acagcacgat taggacggta accgtcccat agaagcaatc cgtacccttg ggtagcagcc
     5101 agttcttttg ccttcaaaag cgattcagcc aactcgtatg tccctacaat gcgatttact
     5161 tcataaccgt caaccggttt tccggtgaaa ttatcccaag tggcatattt agcgtcccaa
     5221 cgaacaccgt gtactatttc atctaaaaaa gtaaatccta tttccatgct tatcacccct
     5281 ttaacgctaa tacgatcaag cggtcaatca gttcgggaag tgcaatacct gcagcggcca
     5341 tcatacgggg ataacgactg tatgacgtga aaccgggcag agtattgact tcgttcagta
     5401 caatgcggcc gttatcttgt aaaaacatat ccacacgggc tagacctcta cagccgagcg
     5461 ctttatatat tttttttgcc gtttcctgta tccgtcctcg ctcctctgct gaaaggtctg
     5521 cgggaacggt tataactgcg ttttcagagc ctttttccgg ctcgacttcc tgatgaatac
     5581 gaaagattcc gtactgcagc ctgatttggt ccacctcgcc aacagctaac gcggcactgt
     5641 ttcccaatac cgcacaaccg acctcacagc ccgaaacagc ctgctcaatt aagattttgc
     5701 tgtcatattg tcttgccgat tcaattgcgt agtccaattc gtccgcgcta ttgacttttt
     5761 tcacaccgaa ggatgagcct gaacgcgccg gcttaacaaa aacaggatag gtaaacgtag
     5821 ctgccaccgg cctatcatct ttattaataa cccaaaaggc gggagtagct atcccagcat
     5881 ttttcgcaac gatgtatgtc aacgatttgt ccatacaaat tgctgagctt tgaatatcgc
     5941 agcctacaaa agggataccg gacaattcaa acagaccttg tatggatcca tcttcacctg
     6001 acttgccatg caaagctgaa aatgctacat caacatggtt gatttcatat tcatggttct
     6061 ttttaacaag taatccgtgc atttttttat ccggcgagag tacagctgaa tagcaattgt
     6121 cgttttccca ttccgcgcaa ggtttttcgc acattttcca tacaccagat ttcgtaattc
     6181 caatgtataa cggctcgtat ttttctttat taatgttagc ggctatctct attgcagatt
     6241 ttaccgatac gtcatgctcc tctgagcaac ccccaaacag tattgcaact tttattctat
     6301 tcatgctcct gtctcctttc aaaatccaaa cagtttttaa tggttttttc aacggtatca
     6361 cgcaacgctt gctcggtata ataggccgta tgcggtgtga ttatcacgtt aggcattctt
     6421 tgaagtttaa gtaaaaattg attatcaatt ggtttttggg tgcaatcaga gtagaaaaac
     6481 tcttcctctc cttccaatac atccaatgcg gcaccgccca gtttcccgtt ttctaatgct
     6541 ttaaccaact cataggtatc tacaagtgga ccgcgcccag tattgataag aaatgctcct
     6601 tgcttcattc tctgtatttg ttcgtggctg ataatatagt gcgtatccgt attgagcggc
     6661 acatgaagcg taacgatatc gctattttgc agcaactcat caaacggtac atagtttacc
     6721 tctatacttc ggctgcgact ataagccaac actttacatc caaatcctcg cagccgctca
     6781 ataaccgctt tgcctatctg gcccgttccc accacaccaa ctgtcatgtc gctgagtacc
     6841 ttgccacggt cgctgtccaa cctgaaatca tgtttttcca cagagcgcac aatcgatttt
     6901 acgttgcgta ctgccataag aattagcatc atagtataat cggcaacgct atccggcgag
     6961 tacgccacat tgtcgacagt gatgcccatt ctcttagcag cagttgtatc tatatgattg
     7021 cagccgatgc ttcgggtaga aatatatttc acaccggctc tcttcagcgc aagaagaata
     7081 gaggcggaaa tctctgattt atgtcccaca ctgatacatt gattgaaagg cgcggatttg
     7141 gcgttggatt ccgacacgtt ggcgttaatt atcgttgcca taacgccaaa gcgaggcgaa
     7201 agagcatgga atgcatctgc ctcatcctgc tcacatccat aaacagtaat gccgatgtta
     7261 ttcataatta agaccaaccc tttctgtgaa aggcaccaaa taaaacgggg atgccaatgg
     7321 taatcttgtc aaatctttct ctgaagaacg aaaacggctc gttcttacgg tattaagtct
     7381 accgtgtaag aacgagccgt ttcttaatat taaattgtta atttcctaag aaaaagtaaa
     7441 gataaccttg agattttcct aaaa
//
LOCUS       DAXWOO010000137         7395 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.140, whole genome shotgun sequence.
ACCESSION   DAXWOO010000137 DAXWOO010000000
VERSION     DAXWOO010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.140"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            67..150
                     /locus_tag="ACGE70_002257"
     CDS             67..150
                     /locus_tag="ACGE70_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFF8693869.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            275..1012
                     /gene="erm(B)"
                     /locus_tag="ACGE70_002258"
     CDS             275..1012
                     /gene="erm(B)"
                     /locus_tag="ACGE70_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFF8693870.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1182..1442
                     /locus_tag="ACGE70_002259"
                     /pseudo
     CDS             1182..1442
                     /locus_tag="ACGE70_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
     gene            1605..2270
                     /locus_tag="ACGE70_002260"
                     /pseudo
     CDS             1605..2270
                     /locus_tag="ACGE70_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558228.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2306..2551
                     /locus_tag="ACGE70_002261"
     CDS             2306..2551
                     /locus_tag="ACGE70_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFF8693871.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            2654..3523
                     /locus_tag="ACGE70_002262"
     CDS             2654..3523
                     /locus_tag="ACGE70_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFF8693872.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3504..4238
                     /locus_tag="ACGE70_002263"
     CDS             3504..4238
                     /locus_tag="ACGE70_002263"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFF8693873.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4271..5179
                     /locus_tag="ACGE70_002264"
     CDS             4271..5179
                     /locus_tag="ACGE70_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFF8693874.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            5176..5718
                     /gene="sat4"
                     /locus_tag="ACGE70_002265"
     CDS             5176..5718
                     /gene="sat4"
                     /locus_tag="ACGE70_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HFF8693875.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            5811..6605
                     /locus_tag="ACGE70_002266"
                     /note="aph(3')-IIIa"
     CDS             5811..6605
                     /locus_tag="ACGE70_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFF8693876.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            6881..>7395
                     /locus_tag="ACGE70_002267"
     CDS             6881..>7395
                     /locus_tag="ACGE70_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFF8693877.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMI"
BASE COUNT         2592 a         1117 c         1554 g         2132 t
ORIGIN      
        1 gtgtataata ggaattgaag ttaaattaga tgctaaaaat ttgtaattaa gaaggaggga
       61 ttcgtcatgt tggtattcca aatgcgtaat gtagataaaa catctactgt tttgaaacag
      121 actaaaaaca gtgattacgc agataaataa atacgttaga ttaattccta ccagtgacta
      181 atcttatgac tttttaaaca gataactaaa attacaaaca aatcgtttaa cttctgtatt
      241 tatttacaga tgtaataact tcaggagtga ttacatgaac aaaaatataa aatattctca
      301 aaacttttta acgagtgaaa aagtactcaa ccaaataata aaacaattga atttaaaaga
      361 aaccgatacc gtttacgaaa ttggaacagg taaagggcat ttaacgacga aactggctaa
      421 aataagtaaa caggtaacgt ctattgaatt agacagtcat ctattcaact tatcgtcaga
      481 aaaattaaaa ctgaacattc gtgtcacttt aattcaccaa gatattctac agtttcaatt
      541 ccctaacaaa cagaggtata aaattgttgg gagtattcct taccatttaa gcacacaaat
      601 tattaaaaaa gtggtttttg aaagccatgc gtctgacatc tatctgattg ttgaagaagg
      661 attctacaag cgtaccttgg atattcaccg aacactaggg ttgctcttgc acactcaagt
      721 ctcgattcag caattgctta agctgccagc ggaatgcttt catcctaaac caaaagtaaa
      781 cagtgtctta ataaaactta cccgccatac cacagatgtt ccagataaat attggaagct
      841 atatacgtac tttgtttcaa aatgggtcaa tcgagaatat cgtcaactgt ttactaaaaa
      901 tcagtttcat caagcaatga aacacgccaa agtaaacaat ttaagtaccg ttacttatga
      961 gcaagtattg tctattttta atagttatct attatttaac gggaggaaat aattctatga
     1021 gtcgcttttt taaatttgga aagttacacg ttactaaagg gaatggagat aaattattag
     1081 atatactact gacagcttcc aagaagctaa agaggtccct agcgcctacg gggaatttgt
     1141 atcgataagg ggtacaaatt cccactaagc gctcgggacc ccttgtagga aaatgtccta
     1201 agtgtggcaa caatattgta ttaaaaaaat cgttttatgg ttgttcaaat tatcctgaat
     1261 gtaagtttac tttagctgaa cattttagaa agaaaaaact aaccaaaacg aatgtaaaag
     1321 aattactgga gggaaaagaa accctggtaa aaggaatcaa aaacaaagag aaaaagccct
     1381 acaatgccgt tgtaaaaatt ggggaaaagg gatatattga ttttatctct ttttcaaaat
     1441 aaacatgggg ctgacccaaa agtagctcct atatgaaaaa tagaggtctt tccatttaga
     1501 tagtagtgga aagacctcta tttatctttt gaaagtgaat acaaaaataa agcacactct
     1561 gctataatat aggtgattaa tccatttgag agaggtgctt tatcatgtat caaaattata
     1621 ccataaacca actttgtttg cctatagatc ttgaaattaa attagaagaa aatgattttg
     1681 ctcatgccat tgtgcagttt gttgactcta ttcccgatga agtctttctt ccttattatc
     1741 aatctatggg tagaccccaa tatcatcctc gtatgctact ttccattatt ctttgtgcct
     1801 atattcaagg ggtttattcg ggccgccaaa tacaaaatat gctgatagat agtattcgga
     1861 tgcgctactt atctcaagag caattcccta actttcgtac tattaatcgt tttcgcgttc
     1921 atccgattat gaacgatatt ctagatcatt catttgttca atttcgtgag ctactggttc
     1981 aaagtggctt aatcagtggc agtgctctat acattgatgg tacaaaaatt gaagcggatg
     2041 cgaataaata ctctttcgtt tggaaaaaaa gtattttaaa gtataaggat agccttgatc
     2101 aaaaagccct tgaaaactac caacaaatgg tggaaacaaa aatcttacct gagttaatag
     2161 atgatttaaa ggatgaactt tctatagaag agattgaaag gattcgtcaa tctttggaag
     2221 aaaaggaaaa tcaattaatt gaagcgattg accaaaccga aacagtagaa gggggctgac
     2281 ccattattaa taaggagtgg acaacatgat tgatgtaaat gagctattgg aagaagctat
     2341 tagagaaact gaaaatctaa atgatggcga agtttttctt gttaaagatt tattcaaggg
     2401 atatgtgggg aataggatac ccagaaagga tcgacttctg cttggaacgt tatttttaaa
     2461 ccatgttagc aaaatgaatg gtaatttaaa ggctattgaa aagacttcat ctaatcagca
     2521 gaggtataaa aagacaattg ataagtttta actatctttt gtgaaattaa aaaaattata
     2581 aatatgttga gtagaagcaa aagatttggc ttggaatttt tacagcagtg aatcgataaa
     2641 aggagcatgc ttgatggtag ataatattat taaatcagta gcagagaaat tatcctctct
     2701 gtcttatata gaaggtattg ttttaggtgg ttcacgtgca aggggcaccc atacagagga
     2761 ttcggatata gatatcggca tctattacaa ttcagaatca tttgacataa atactattaa
     2821 tcaattcgct acaaagctgg atgatgagca tagaaataac cttgttgtac ctcccggagc
     2881 atggggtgat tggattaatg gcggcggatg gttagtcata aacgggtatc atgtggattt
     2941 aattttacgt gatattaaac gtgtggaaca aataatgaaa gatacagagc acggaattgt
     3001 tactgccaat tatcagactg ggcatcccca tggttatatt agtgcaatgt atcgaggaga
     3061 attagcgatt agcaaaatac tatatgctaa gaatgaaagc ttatgcgaat taaaaaaaca
     3121 ggcagaaact tatcccaatg ctttgcagaa aagtttagtt aactttttta tgtttgaagc
     3181 agggttctct ttaatgtttg taaaagcaaa ttcgggaaca gacgataaat attatattgc
     3241 gggtcatgtt tttcgtatag tttcatgttt aaatcaagtg ttatttgcat gtaataatgc
     3301 ttattgtatc aacgaaaaga aagctataaa actgcttgaa acttttgaac ataaacctga
     3361 aaaatatacc gagaaggtaa atcatatttt tgaagtactc ggtatctcac tttttgaatg
     3421 ctacgacatg accgagaagc tttataatga agtgaatgaa attgtatcgg agataaataa
     3481 ctttttaaac gaggagagtt cagatgaaag aaaacaaata tgatgataat atattttttc
     3541 aaaaatacag tcaaatgagt cgctcgcaga aaggactggc tggtgcggga gaatgggaga
     3601 ctttgaaaaa gatgctacct gattttaagg gtaagcgtgt gcttgattta ggatgcggct
     3661 atggatggca ctgtatatat gcgatggaaa acggtgcttc ctctgtagta ggtgttgata
     3721 tttctcataa aatgctcgaa gtagcaaaag gaaaaaccca ttttccacag attgaatatg
     3781 aatgctgtgc catagaagat gtggatttcc cagaggagag ctttgatgta atactaagtt
     3841 cgcttgcgtt tcattatgta gcagactatg agaatttaat aaaaaagata tataggatgc
     3901 tgaaggctgg tggcaattta gtttttacag ttgaacatcc tgtttttact gctcatggaa
     3961 cacaagactg gtattataac gaaaaaggag aaatactgca tttcccggtg gacaattatt
     4021 attatgaggg caaacggaca gctatgtttt tggaagaaaa ggttacaaaa tatcatagaa
     4081 cactgaccac atatctaaat acactgcttt caaatagttt tataataaat cagattgtgg
     4141 agccacagcc gccagagaac atgatggata ttccggggat ggcggatgaa atgcgacgcc
     4201 caatgatgct gattgtatcg gcaaaaaaga agatgtaata atatagaaaa aataaacgag
     4261 gagtatgtaa atgagatcag aaaaagaaat gatggattta gtactttctt tagcagaaca
     4321 ggatgaacgt attcgaattg tgacccttga ggggtcacgc gcaaatatta atatacctaa
     4381 agatgaattt caggattatg atattacata ttttgtaagt gatatagaac cgtttatatc
     4441 taatgatgac tggcttaatc aatttgggaa tataataatg atgcaaaagc cggaggatat
     4501 ggaattattc ccacctgaag aaaagggatt ttcctatctt atgctatttg atgattacaa
     4561 taaaattgat cttaccttat tgcccttgga agagttagat aattacctaa agggcgataa
     4621 attaataaag gttctaattg ataaagattg tagaattaaa agggacatag ttccgactga
     4681 tatagattat catgtaagaa agccaagcgc aagggagtat gatgattgct gcaatgaatt
     4741 ttggaatgta acaccttatg ttattaaagg attgtgccgt aaggaaattt tatttgctat
     4801 tgatcatttt aatcagattg ttcgccatga gctgctgaga atgatatcat ggaaggtcgg
     4861 catcgaaaca ggctttaaat taagtgtagg caagaactat aagtttattg aaaggtatat
     4921 atccgaggat ttgtgggaga aacttttgtc cacctaccgg atggattcct atgaaaacat
     4981 atgggaagca ttatttctat gccatcaatt gttcagggcg gtatccggtg aggtggcgga
     5041 aaggcttcat tatgcctatc cggagtatga taggaatata acaaaatata ccagggacat
     5101 gtataaaaaa tacactggta aaaccggctg cctggatagc acatatgccg ctgatataga
     5161 agagaggcgg gaacagtgat tacagaaatg aaagcagggc acctgaaaga tatcgataaa
     5221 cccagcgaac catttgaggt gataggtaag attataccga ggtatgaaaa cgagaattgg
     5281 acctttacag aattactcta tgaagcgcca tatttaaaaa gctaccaaga cgaagaggat
     5341 gaagaggatg aggaggcaga ttgccttgaa tatattgaca atactgataa gataatatat
     5401 ctttactacc aagacgataa atgcgtcgga aaagttaaac tgcgaaaaaa ttggaaccgg
     5461 tacgcttata tagaagatat cgccgtatgt aaggatttca gggggcaagg cataggcagc
     5521 gcgcttatca atatatctat agaatgggca aagcataaaa acttgcatgg actaatgctt
     5581 gaaacccagg acaataacct tatagcttgt aaattctatc ataattgtgg tttcaaaatc
     5641 ggctccgtcg atactatgtt atacgccaac tttgaaaaca actttgaaaa agctgttttc
     5701 tggtatttaa ggttttagaa tgcaaggaac agtgaattgg agttcgtctt gttataatta
     5761 gcttcttggg gtatctttaa atactgtaga aaagaggaag gaaataataa atggctaaaa
     5821 tgagaatatc accggaattg aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg
     5881 aaggaatgtc tcctgctaag gtatataagc tggtgggaga aaatgaaaac ctatatttaa
     5941 aaatgacgga cagccggtat aaagggacca cctatgatgt ggaacgggaa aaggacatga
     6001 tgctatggct ggaaggaaag ctgcctgttc caaaggtcct gcactttgaa cggcatgatg
     6061 gctggagcaa tctgctcatg agtgaggccg atggcgtcct ttgctcggaa gagtatgaag
     6121 atgaacaaag ccctgaaaag attatcgagc tgtatgcgga gtgcatcagg ctctttcact
     6181 ccatcgacat atcggattgt ccctatacga atagcttaga cagccgatta gccgaattgg
     6241 attacttact gaataacgat ctggccgatg tggattgcga aaactgggaa gaagacactc
     6301 catttaaaga tccgcgcgag ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac
     6361 ttgtcttttc ccacggcgac ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa
     6421 gtggctttat tgatcttggg agaagcggca gggcggacaa gtggtatgac attgccttct
     6481 gcgtccggtc gatcagggag gatatcgggg aagaacagta tgtcgagcta ttttttgact
     6541 tactggggat caagcctgat tgggagaaaa taaaatatta tattttactg gatgaattgt
     6601 tttagtacct agatttagat gtctaaaaag ctttaactac aagcttttta gacatctaat
     6661 cttttctgaa gtacatccgc aactgtccat actctgatgt tttatatctt ttctaaaagt
     6721 tcgctagata gagttctata ttagagatgt aaagagtttt ggtgaagagg tttgtgagta
     6781 tggcttttat aaccagggtg caatgagaag cactaacagc agctagctta agaacgcata
     6841 ggatatactt tttatggaag tcacagagga ggaataaaat ttgaacagga ttaacagagt
     6901 aacctctatt cttattcagc tgcaatcaaa aaaaataatt cctgccaaag aaattgcaca
     6961 gcgatttaat ataagcttaa ggacagttta cagagatatc cggacacttg aagaagccgg
     7021 aataccaatt ggatctgagg ccggaaaagg atattttctt gtagagggct tcctacttcc
     7081 gccggtaatg tttacagcgg cggaagtggg tgcattgatt acagcaggga aatttttaaa
     7141 ttgccatgga gatgaatcat ttataaagga ttttgattca gctatgtata aaatcaaatc
     7201 tattttgaag catggtgaaa aaaactatgc acaggagttg gagaacagta ttaacgtgta
     7261 cagcacatct ggacagaaaa atacattggc ggataacgtt attgcagcaa ttcagactgc
     7321 aatctgcaat aaaagggtaa tatcaattca atatcctgca tcaggagggc aagaaccgga
     7381 aagcagaatg ataga
//
LOCUS       DAXWOO010000138         7372 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.141, whole genome shotgun sequence.
ACCESSION   DAXWOO010000138 DAXWOO010000000
VERSION     DAXWOO010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.141"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..570
                     /locus_tag="ACGE70_002268"
     CDS             <1..570
                     /locus_tag="ACGE70_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFF8693878.1"
                     /translation="ASRIAIMSEGRVIQFDTPENILRHPANDFVEELIGEDRLLQAKP
                     NATRVGEVMLNTAITITPEKSLQEAIKLMREKRVDTLLVTDNSNVLKGFIDVETLNKK
                     RGKVSSVGDILNKHVFYVKESAYLRDTLQRILKRGLKYVPVVDEQNKVVGILTRASLV
                     DIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            574..1215
                     /locus_tag="ACGE70_002269"
     CDS             574..1215
                     /locus_tag="ACGE70_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8693879.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            1212..2129
                     /locus_tag="ACGE70_002270"
     CDS             1212..2129
                     /locus_tag="ACGE70_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFF8693880.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            2126..2797
                     /locus_tag="ACGE70_002271"
     CDS             2126..2797
                     /locus_tag="ACGE70_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8693881.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(3338..3826)
                     /locus_tag="ACGE70_002272"
     CDS             complement(3338..3826)
                     /locus_tag="ACGE70_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFF8693882.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(4027..4293)
                     /locus_tag="ACGE70_002273"
     CDS             complement(4027..4293)
                     /locus_tag="ACGE70_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFF8693883.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(4293..4556)
                     /locus_tag="ACGE70_002274"
     CDS             complement(4293..4556)
                     /locus_tag="ACGE70_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFF8693884.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            5077..5217
                     /locus_tag="ACGE70_002275"
                     /pseudo
     CDS             5077..5217
                     /locus_tag="ACGE70_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(5359..7182)
                     /locus_tag="ACGE70_002276"
     CDS             complement(5359..7182)
                     /locus_tag="ACGE70_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFF8693885.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
BASE COUNT         2211 a         1552 c         1414 g         2195 t
ORIGIN      
        1 gcaagccgta tcgccatcat gagtgaaggc cgtgtgatcc aatttgatac accagaaaat
       61 attttacgtc atcctgcaaa cgatttcgta gaagagttga ttggagaaga tcgcctacta
      121 caagccaagc caaatgcgac aagagtcggc gaagttatgc taaatacagc aatcacgatt
      181 acaccggaaa aatcattaca agaagcaatc aaactgatga gggaaaaacg tgtagatacg
      241 ttgttagtta ccgataattc gaatgtatta aaaggattta tcgatgtaga aacgttgaat
      301 aaaaaacgag gaaaagtatc cagtgtcggc gacattttga ataaacatgt tttttacgta
      361 aaagaatccg cctatcttcg tgataccttg caacgcattt taaaacgcgg gctgaaatac
      421 gtgccggtcg ttgatgaaca gaacaaagtc gtaggtatcc tgactcgtgc atcattagtg
      481 gatatcgtct acgatgtgat ttggggagaa gatcccgcaa ctactgatgt tgcagagtca
      541 gctactcctg aagaatctaa ggaggtctga cctatgcagc agttttttca tcagcacggt
      601 tcagaaattc tctcaaaagg ttttgaacat ctttatattt caggacttgc tttgatcatt
      661 ggtatcatca ttgctgtacc gcttggtgtc ttactgacac gttttccaaa agctgccacg
      721 attgtgattg gcttaaccag tgccttgcag acaatccctt ctcttgcttt attggcattg
      781 atgatcccgc tatttggcgt aggtaaatta cctgcgatta tcgcactctt tatttattca
      841 ttattgccga ttttacgtaa tacgtacatt gggatgaaaa atgtcgattg gaattatcgg
      901 gatgtggcaa aaggcatggg gatgactgag ttccaatcca ttttttccgt tgaattgccc
      961 attgctatgc caacgatcat ggctggtatt cgtttagctg ccgtttacgt gattgcatgg
     1021 gctacacttg cctcttatat cggcgctggt ggtttaggcg atttgatttt tagtggactg
     1081 aataattacc aaccagatct cattttggca ggtacgatcc ctgtaacaat tttagcacta
     1141 ttggctgatt ggcttctagg tctcttggaa caccggctga caccgatcgc acttagagag
     1201 gagaatgaag aatgaaacgt atactaaaaa tgaccatggt tcttttctct cttcttatac
     1261 ttgcgagttg ttccttccct ggtcttgcca gcaatacaga tgaagacacc atttcgatca
     1321 caggtggaat cacttcagaa gcacagatct tagcaagtct agttgccgga atggttgaac
     1381 attatacaga taaaaatacc gcaatcatca acaatctggc tactacaact atcaaccatc
     1441 aagcgatgat gaacggagat gcttcaattt cagcggctcg ctacacagga acagatttaa
     1501 cgactacatt gaaccttcct cctgaaaaag atccgaaaaa agcgtttgct accgtgaaag
     1561 atgaatttga gaaaagatac ggtcaaacgt ggttcccgtc ctatggtttt gaaaatacgt
     1621 atgtctttct tgttcgaaaa gacaccgcac aaaaatacca tctatctaaa gtcagcgatc
     1681 tgaaaaatgt agcggatgaa ttagtagctg gtgtagatac ttcttggatc aatcgtaaag
     1741 gagacggtta tgacggcttc caagaaactt atggcttctc cttcaattcg atattaccta
     1801 tgcagattgg acttgtttat gacgcagtcg aagcaggaaa aatggatatt gttcttggct
     1861 actctacgga tggacggatc gccagttatg atctagtcat gttggaagat gatcgtcaat
     1921 tcttcccacc atatgatgct gctccagttg tagacaacaa gatattagct gatacgcctc
     1981 atttaaaaga agccttaaat aagttaggtc ataccatcag tacggaaaaa atgcaggaat
     2041 tgaactacga agcagataat aatctagtgg aaccatcagt agtagcacaa cgtttcttac
     2101 aagaaaacca ctattttgaa gggaagtgaa gcaattggat aaaatgaatg tctttcaaca
     2161 atttttctat tatttccagg aaaatggctc ctatatcttt gcacaattta tccgtcactt
     2221 cctgatttct atctatggtg tcctctttgc agcagttgtc gggattccgg ttgggatcat
     2281 gatttctagg agacgtaaat tagcaaactg ggtgattcgt ttggcgaata tcatccagac
     2341 gatcccttct ctagccatga tctccatttt aatcattgga ttaggcctgg gagtcaatgt
     2401 cgtgattgtt actgtatttt tgtattccct cttaccgatc atcaagaata cgtataccgg
     2461 gatgatccaa gtagacaaaa acatccttga tgtcggcaaa ggcatgggga tgactgctag
     2521 acaacgacta ttcatggtag agttaccact ctctgtttct gttatcatgg caggcataag
     2581 aaacgcgtta gttgttgcca tcgggataac cgcgatcggt gcattcgtcg gtgctggtgg
     2641 tcttggagat atcattatcc gaggaaccaa tgcaacagag ggcacatcga ttattttagc
     2701 aggtgcattg ccaactgcat taatggcgat catcacggac tggcttttag gtattttgga
     2761 aagacggcta gatccagcca gccgtacatc acgctgatgt tattgtaaaa aacaaaagct
     2821 tctgcgacca tttgttggtc gcaaaggctt ttttagttct gtcattttta aggcaataat
     2881 ccactctgat tcttccctcc ttatgggcaa agagttgctt ttcttagcta tttatccatt
     2941 ccgtatactc caacttcata ctgccaacgg gttcatatct gcatccaccg actaagtttt
     3001 tttcttatcc gctaaggcat tttgccaatc cattttccca tcactcgtca tggaattcat
     3061 accacatgat ctctaaatac tcgtctgctt gttctttgaa ttgggcatca attcttacat
     3121 cctttggcgt ggctttcatt taagcctttt ccatcagtcc tcgattgttc gatgccttca
     3181 gctgcatcat caaaactggt gactgccgta tcggtaactt cgatcatctt ttatacaatc
     3241 gggaacttgc catcctcatc cttggctaaa aacatatcta gccattccag cttctcgcca
     3301 tgggaggttt gcggttccta aaaccggtac acgattctta tggactgcat ccgtcgcaac
     3361 tagacttggc gatacgatga tccttcacca gaagagcacc cccgtaaacc aattgatcga
     3421 tatattgcca ataagaaaag acattggcgt aaaagatatc gatccctcgt gatgcattgc
     3481 cgttgatact gctattcatg gccgaaagcg ccacgacttt aacttcttcc gtttgagtga
     3541 cattcatgtt cggcaatgtc gctttggata cccgctctgc taaaggaacg acactggtat
     3601 tgtatttcgt atctgtatct tttccaaaag accatgccaa cgaatcctct gggaaccaat
     3661 aagacgactc cggttgattg tccattgctt ttttcaacat acttttggct gtttctcgat
     3721 aaacgacatt tccaattgtc ttcgcctcac tttggcgata atcgatggtc gttgccactg
     3781 ccaacactaa caacgatgct ccaattttac tgattttcct tgtcacactt cttcctcctt
     3841 gttttacata aacccgtcaa cattccattc ctaaactcaa acaatgaccc ttcaaaggtg
     3901 gagatatagg aacaaattaa agaaatcatg caataaatcc aagaaggttg gttttagaac
     3961 aaaaaaagag tctccttttt ggggagactc tttgttaact atctaaaaag gttggctctt
     4021 ctcacttcac tcataatgtg tccaggcaga ataaatttct acaactttat tttcctcatc
     4081 aactttgtaa accactcgat gctgtctatt cagcctgcgt gagtatctgc cttcatgtgt
     4141 aggtctcagc ttttcaaaag actgcgtggg catatatgga tcttccttca atgtctgaat
     4201 cacttcttca aattgtttct ttaaattcgt ttgcttgatt tttcttaaat ccactttcgc
     4261 agagtttttg attgcgactg tatagttact catcaaagag cttcccaatc aatatcatcg
     4321 atattcgtaa agctactgtc gtctttttcg cggtctcgta ctttatctaa agtacctgtt
     4381 tgctcaagaa aaagggtttc ttgaatcgct ctccagtctt ccagcccaat caatacagca
     4441 ttgtttccac tacgatcact aataatctcg atttcagtat gattttcatt tacctctttc
     4501 aataactgat aaaaattttt acgagcagtg ctaggattca tcgttgtatt agccattgtc
     4561 tctcctcctt tttctgatat aattatagcg tacttgatta agtacgtcaa aaaaatacaa
     4621 taaagcactt aaattcagca aacttttcct cttttttaca tagagaaaaa gacccctttt
     4681 tagggaggat ctttgtccac gctcacttct tactccttta cgactagcgg tagccaaacg
     4741 taaagtaatt ggtaacaacg tatcatcttt agcaaaggtc gaatccaaag cctgtgagtc
     4801 tatgcaacaa caccaataca cgaaggttcc tcgttgcagt gtgacttgat tgatatcttg
     4861 aataacctga ggatacgcac gaaagatttg taacggttat tcaaaactga ttttggccgt
     4921 caagtcttct actgcctcaa aaatgattgc ctctttcgtc tcctgtcatt catgttcact
     4981 atcgatttga tacttgctta gaccgttggg tttgtgaatc accaattaaa aatggcactg
     5041 agttatggta acaaaaaatg gtcacttgac gaggctgaga ttcctactta tgaagacaca
     5101 tggcacttaa gagtcgaggt aaaggaagaa gctgaaaata taggaggctg tacattattt
     5161 ggcaaaggat tcggaaatta tgatcaagac atcaaaatca aaatgtttta cagctaaata
     5221 ggaagaataa taacatttca tacgctgttc ccatctttaa tggcttatag agggcacctt
     5281 tagctgagaa taagacaaaa cagaccctct agcttcaatt aagagctaga gggtctgttt
     5341 ttgattttaa aacttggttt atttcttcaa atggtagcta taggtgctga caacgatatt
     5401 ttcacgccaa gacaatcgca atcttaaccg caaacttgtt tggttatgga gaatattctg
     5461 ccagcgtctg tttggtacaa attgaggaat caacacagta gttgtatagt tttgtttcgc
     5521 tgcattttta cttacgatat ctacataacg gataattgga ttttcaatcg agcgataaga
     5581 agagtggacg attgaaaaac ggacatctgg gaaatgcttt ttaaattctg cttcgatttc
     5641 tttttctttt tcgacatctt cgtctagcga aacatgcatc gcaacgacat aatcaccgat
     5701 cgaacgtgcg taattcaacg caccgatatt tacccgtgtc acattcccga ctaatacaag
     5761 aacggtattc ccgtcataat catgtagttt tgcatcattt tctaaacgaa gctgttctgc
     5821 tactttttta taatgatcat ggatcttata aaaaatgaac ataacgattg gcataatgat
     5881 gaagaaaggc caaatgtccc ctaaccgata gacaaacaaa atcgcaatga ttgcatatga
     5941 gatgaacgca cccgtaatat tggcaatcga tttgcttaac catctttttc cttctttttt
     6001 ccatttgacg accatccctg tttgagataa agcaaatgga atgaacacgc cgatagagta
     6061 taacggaatc aagcgttctg ttgagccatg gaaaataaac agcaatacga tcgaacctag
     6121 cgctagtgtg ataatcccat tggaatagcc tagtcgatcc ccacgatctt gatacatgtg
     6181 aggcatgaat ttatcttttg ccagattgta tgcaagaact ggaaaagcag aaaaaccggt
     6241 gttagcagca actgctaaaa tcagcgctgt agcaaattga aggacgtaat acaaaatacc
     6301 atgtccaaag acagctttac caatttgaga caaaacagtg acttcttttt caggaacgat
     6361 cccataccaa tagttgataa atgtgatccc cacgaagaag aaacctaaga tcaatgccat
     6421 catcgtcaat gtagcagcgg cattttttgc tcttggcttc ttaaagaatg gtacagcatt
     6481 actaatcgcc tccaccccag ttaaagagga agaaccagaa gaaaatgctc gtaaaatcaa
     6541 agcgatagaa acacccggca cagccgctcc tggaagtgca gtagcttgca atggcacagc
     6601 acctgtcacg atattatata aaccaacaac gatcagtaac gagatcacaa ggataaatgt
     6661 gtacaccgga aataatagga acgatgcaga ttccctcagc ccacgaagat tcagcatcat
     6721 cagtagcaaa acgattgtca cagaaatagc gacttgatga ccgtagagtg ctggcaccgc
     6781 tgatgtgatc gcttcagcac ctgctgagac agatactgct actgtcaaca tgtagtcgat
     6841 caataaagag ccaccggaaa tcaagccagc attctttcca agattctcac tgctgacgac
     6901 atatgctccc ccgccatgag gataagcatg gatgatttga cgatatgata gcgttaatga
     6961 aatcaataaa ataataacaa aagcggcaat gggtagcgaa taccagatgg ctgcagcaga
     7021 caaagcgact aaaacgacaa cgatttgttc cgtaccatat gcaatagatg ataacgcatc
     7081 agacgacaat aatgccaaag cagcaaatcg ggttaatttg tgctcatcat tttcagcaga
     7141 ttttaaaggc ttacctacta acaacctttt taagtaatcc aatttctaaa ctcctttaca
     7201 cacaatttta cttcaagttt tatagtcgaa aatttatagt aaacaagatg ttatagtacg
     7261 ctcactgacc ttaaaagtca ataaacttta agaaaataat tggaaaaaaa cagaaaaata
     7321 tcaagcgctt tttcaaacgc tttaatacag caaaaatcta acattttttt at
//
LOCUS       DAXWOO010000139         7304 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.142, whole genome shotgun sequence.
ACCESSION   DAXWOO010000139 DAXWOO010000000
VERSION     DAXWOO010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.142"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            150..797
                     /locus_tag="ACGE70_002277"
     CDS             150..797
                     /locus_tag="ACGE70_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFF8693886.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    926..1142
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1190..3835
                     /locus_tag="ACGE70_002278"
     CDS             1190..3835
                     /locus_tag="ACGE70_002278"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFF8693887.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4145..5458
                     /locus_tag="ACGE70_002279"
     CDS             4145..5458
                     /locus_tag="ACGE70_002279"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFF8693888.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5448..6101
                     /locus_tag="ACGE70_002280"
     CDS             5448..6101
                     /locus_tag="ACGE70_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFF8693889.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6156..6848
                     /gene="radC"
                     /locus_tag="ACGE70_002281"
     CDS             6156..6848
                     /gene="radC"
                     /locus_tag="ACGE70_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFF8693890.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6998..7198
                     /locus_tag="ACGE70_002282"
     CDS             6998..7198
                     /locus_tag="ACGE70_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFF8693891.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
BASE COUNT         2407 a         1239 c         1604 g         2054 t
ORIGIN      
        1 tacgtttggt tgagcttatt accgcagatg caagcttttg aacaccgttt tatttgtctc
       61 acaccctttt ccttcctact tgtaaccttg ctcccgtgtt agaaacatgt tacactaaat
      121 atgtgaaatt attaacaagg agcgtcgtta tgcgagaaaa aacagataca ctgccaaaag
      181 caactgcgaa aagattacct ttatatttac gctacttgaa gatgctggat gactcaggta
      241 taagtagaat caaatcaaat gagttcagcg aaatcaccca aattccttct gcaacgatcc
      301 gcagagactt ctcgcaatta ggcgagctag gcagaagcgg atatggctat gatgtccctt
      361 ttcttatcga tgtattcaat aatatcctta atactaaaga agaaaaacga atcgcattag
      421 taggatatgg aaatcttggc aaagcattga aacacaacaa ttttagaaga aatgaaaatc
      481 taaatatcgt gtgcgtcttt gataatgatc cagcactgat caatcgagta attgatggag
      541 aaatgatcta tccgattgat cgtttcgcgg agatagccaa agcaaaaaat gtgacagtag
      601 caatctctac ggtaccaagt aaatattcac aatctgctat tgatgaaatt gtcaaaggaa
      661 atgtaaccgc tatacttaat tttgcgcctg acagagtaac tgtcccagct tatgtcaatg
      721 ttcaatacat tgatttgaca accgagttgc agacgcttat ttactttgat gagaattact
      781 cagaagtttt ttcttaggaa aatttgagta ggcttattga tcttgcgaga actcgtcgta
      841 attctttcaa aaatttttta gtactaaatg aagtggtttt ttcacgacca cttgttgact
      901 aaaaaaaaaa accattgtag aatgaagcat aacgctgaac aagaggagta actggcacat
      961 tcattcttag agagagaact atctgctgaa aggttctcaa tgacaaccag tgaaggtagc
     1021 ttgggagttt ctgtcttgaa tggagtagag atagacgagt cgtgatcgtt aactcacttg
     1081 agaggcaata atgattgatt attgcaattt aggtggtacc gcgtaaatca tacgtcctag
     1141 aatacgaaag tattttagga cgttttttag tttaaaggag gaaaacagca tgacggaaga
     1201 aaataaccta tcaacaaaat ataatccgca agaagttgaa gctggacgtt atcagcattg
     1261 gttagaacaa gatttgttta aaccgagcgg agataaaaaa gcaaaacctt attcaatcgt
     1321 aatcccgccc ccaaatgtta ctggaaaact tcatttaggg catgcttggg atacgacctt
     1381 gcaagatatg ctgattcgtc aaaagagaat gcaaggatat gatactttat ggctgcctgg
     1441 tatggaccat gcaggaatcg cgacacaagc aaaagtagaa gcgaagctta gagagcaagg
     1501 gatttctcgt tatgatctag gtcgtgaaaa gttcatcgat caagtgtggg aatggaaaga
     1561 agagtatgcg agccacatcc gcgaacagtg ggctaaaatg ggcttatctt tagattacag
     1621 tagggaacgt tttacattag atgatggatt atctgaagca gtacgtaaag tttttgttac
     1681 cttatacgaa aaaggtttga tttatcgtgg tgaatatatc atcaactggg atcctcaagc
     1741 acgaacagct ctatctgata tcgaagttat tcataaagat atcgaaggcg ccttttatca
     1801 tatgagctat gaactagcag atggatcagg cgtagtagaa attgcgacaa cccgtccaga
     1861 aacgatgttg ggagatacgg cgattgctgt tcatccagaa gacgagcgtt atcaagcgct
     1921 catcggcaaa aaagtgattc tacctttagt caataaacaa atcccaatca ttgcagatga
     1981 atatgtagat atggaattcg ggacaggtgt agtaaaaatc actccagcac atgatccaaa
     2041 cgactttgag gtaggtaatc gtcatgatct tccacgcgta aatgttatgg atgatgatgg
     2101 tacgatgaat gaactagcag gaaaatatgc tggaatggaa cgttttgctg ctcgtaaagc
     2161 aatcgtgcaa gatctgaaag aaatcggtcg attgattaaa atcgatacga tgatccacag
     2221 tgtgggtcat tccgagcgga caggtgtcgt tgttgaacct cggctgtcta ctcaatggtt
     2281 tgtaaaaatg gcgccgttag cgaaaaaagc cattgataac caagaaacag atcaagcagt
     2341 ggaattcttc cctccaagat tcaatcaaac gtttttacgt tggatggaaa atgtccatga
     2401 ttgggtaatc tcacgacaat tatggtgggg acatcaaatt cctgcatggt atcacaaaga
     2461 aacaggtgaa atgtatgtag ggatggaagc accagcggat agtgaaaact gggtacaaga
     2521 tcctgatgtt ttagacactt ggtttagttc agctttgtgg cctttctcaa caatgggctg
     2581 gccagatacg gaaagcgaag attataagcg atacttccct actagcactt tagtaaccgg
     2641 ttatgacatc attttcttct gggtaagcag aatgattttc caaagcttgg aatttactgg
     2701 cgaacgtcca tttgagaatg tcttgattca tggattgatt cgtgacgaac aagggcggaa
     2761 gatgagtaaa tcattaggta atggaatcga tccgatggaa gtaatcgaaa agtatggagc
     2821 agatgctctc cgctggttct tatcgaatgg ttctgcacca ggacaagatg tccgcttcag
     2881 ctatgaaaaa atggatgctt cttggaactt tatcaacaaa atctggaatg cttcacgttt
     2941 cgttatcatg aatatcgaag gaatgaccgt agaagagatc gatttatctg gagaaaaatc
     3001 agtggctgac cggtggatcc ttactcgttt gaatgaaacg atcgaacgag taactgagct
     3061 atttgatcgt tttgaatttg gtgaagcagg tcgacaactg tataacttta tctgggatga
     3121 tttttgtgac tggtacatcg aaatgagtaa agaagtactt tatggagatg atgctgtttc
     3181 aaaacaaacc acccgtagca tcttagtcca tacacttgat cagatcctac gtttattgca
     3241 tccaatcatg ccattcgtta cagaagaaat atgggaacat attcctcatc agggaaattc
     3301 tctagtagtg gcagaatatc ctgttgtacg tccagagttt gatgatgaaa cagcttctaa
     3361 agggatggaa gtattgaaag aattgattcg agccgttcga aatattcgtt cagaagtgaa
     3421 tacaccattg tctaaaccaa ttacgttgct aattaaaaca aacgatgcta aaatcgatca
     3481 atttttagtt gaaaatacaa gctatatcga acgcttctgt aatcccgaag agttgacgat
     3541 atcaagtgag atcactgcgc cagatctagc gatgtcagct gttctgacag gtgcagaaat
     3601 cttcttgccg ttagcaggat tgatcaatat tgaagaagaa atcaaacgat tagaaaaaga
     3661 actggaaaag tggacaagcg aagtaaaacg tgtccaagga aaactttcta atgaacgttt
     3721 tgtttctaac gcgccggaag aagtagtaga ggcagagcgt gcaaaagaaa aagattattt
     3781 agagaagcaa acggctgtca aagaacgaat cgaaagtttg cgtactattc aataagaata
     3841 gaaaaagaga gtgtgaaatc agctagctat tactaaaagc tagctgattg aacatcgtta
     3901 caggaaagag tttgagcctc actcttagct tcgattcata aggagatgtg acataaattt
     3961 tgtcgcatct cttttttagt ctactcttat tatgtaggag aaggagggag gtggatcatg
     4021 tatcaagagt caataaagta gttgataaaa tcgttcctct ctctgtttct tttctctttg
     4081 tcatactttt atttttctta tgtgttctat ttgttataat aaaagagaaa tgggggaatt
     4141 gttgatgacg attgaagaag caatagaatg gatccatagt cgattaccat ttggttcacg
     4201 tcctggcctt gaccgagtcg aagctttatt agaagtggta gggcatccag agagaaaagt
     4261 aaaaacgatc catatcgctg gaacaaacgg aaaaggttct actgtagctt atttgagaag
     4321 cctattagaa gaggctgggc tgacagtagg tacatttact tcaccttata tcgaaacgtt
     4381 caatgaacgg atcgcaatca atggcaaaag cattccagac aatgatttga tccagcttgt
     4441 aaaaaaatat cagccactga tcaaacaact tgatcaattt gaagcagtag ctggtattac
     4501 tgaattcgag acattaacag caatggcatt tgattatttt ttgatgaaga aagtggacgt
     4561 agcgattatc gaagttggct taggcggctt attggattct accaatgtag ctgtaccaat
     4621 gctgacggga ataacaacga ttggacttga tcataccgaa atacttggag atacaattga
     4681 agaaatcgct gcacaaaaag ctggaatcat caaacaaggc gttccagtag taacaggaaa
     4741 tattgcccca gaagctttta ctgtcatcca acaaacagct aaagcaaaaa atgcaccgct
     4801 ttatgtatac ggggaaaatt ataaaataaa ctatctgcat cctgatgaga tgtggggaga
     4861 agtatttgaa ttttttaatg aaataggaaa gatccctcaa ttaaccactc cgcttttagg
     4921 gcaacatcaa gctgaaaatg caggagtagc catcgaattg tattttgttt actgtcaatt
     4981 ggaaaagctg cctttccgag aaaaagatgt tttgaaggga ttgaagaaat ctcgatggcc
     5041 aggaagaatg gagcgtttga gtaacgagcc attagttgtt ttagatggtg cccataatac
     5101 gcatgcaatg aaccggcttg ttgagaattt gaaaaaagaa ttcaaaggat atcaaatcaa
     5161 cattttgttt tccgccttac gaactaaaaa tgttagagaa atgctagaac gattgcagca
     5221 agtaccaaat gtccatattt atttgacgac ctttgaacat ccaaaagcca tcgaattaag
     5281 tgaagtatca gatttagcaa gtgagagagt atcaatcgtt tcattgtggc aattcggatt
     5341 agcagatata ttggaaaaga tgacaaatga agacgtatta gttgtgaccg gttcacttta
     5401 tttcatttca gaagttcgcc atttattaat acaattagga ggagatcatg aagtataatg
     5461 cagtgatttt tgacatggat ggtttgctgt tcgacacaga gttagtgtat tacgaagcca
     5521 cacaagtagt agcagacaag atgggttttc cttatgataa agacctgtat ttaaaattct
     5581 taggtgtttc agatgaagaa gtatgggcga attatcatat gattttctcg gaatttggca
     5641 aaacaagtgt acaaagattc atagatgatt cataccagga aacgcttgaa cgtttttcgc
     5701 aaggagcggt acaaatgaag cccggtgtgt tggaactcct ctctttttta gaagaacatc
     5761 agattcccaa agttgtcgct tccagtaacc aacgagcagt cattgaactt ttattgaaga
     5821 agaacaatct gtttgaacga tttgagacga ttgtttctgc agaaaatgtt caacgagcaa
     5881 aaccagaccc agaaattttc ttgttagcta atcagaagct aggtacagcg aagaaagaaa
     5941 cattgatttt agaagattca caaaatggta ttttagcagc ttctgcggct gagatcccgg
     6001 tagtcatggt accagatctg cttccaccat cccaagaatt gatcaaaaaa acagctgggg
     6061 ttgtttcttc tttacatgaa atccctgcat tgttaaaata aaaatccatc tcttttcgta
     6121 tcttatttta gataagatag aaaggagatg tttttttggg aaaacgattg atcaaagaag
     6181 tacctactag ctctttacca agggaacgta tggaaattta tggcgcggaa gctttatcgg
     6241 atcaggagct tttggcaatc ttactgcgta ccggtcaaca cccttatagt gtgatgtcga
     6301 ttgctggaaa tctgttaaaa acgtttggag gactggcctc tttacgacag gctactttgc
     6361 atgagttaga agagattcga gggataggac gcgtgaaagc aatcgaaatc aaagccttga
     6421 tcgaattagg tcgacgaatt cagacggatc atgaaagagc ggctccaccg gttcgtacca
     6481 gctacgaatt ggcccagcaa ttgattctgg aaatgaaaga tcacaaacaa gaacatttgg
     6541 tttgtattta tttggacacg aagaatcaag tgatcttaaa aaaaacagtg ttcatcggtt
     6601 cgctgaatca gtcagtcgct catcctagag aaattttcca ttatgctgta cgttattgtg
     6661 cagcaaggat cgttttagcg cataatcatc cttctggtaa tgttattcca tcgcagcagg
     6721 atatgaattt taccaaaagg atacaaaagt gtggagaaat gatggggatc actgtactgg
     6781 accacttgat catcggacgc aaacgatatt ttagtttgag agaagaagga atgatggaag
     6841 agaagtaagt tgctttcttg caaaaaaaat gaagcaagtg tacaattata ttgtaatttt
     6901 gtttgggaat agaagttgaa acatctgttg cacctctatc gtacacgagc aatgttacaa
     6961 tttaaattag tgcttttggc acgaggagga tattattatg gaaaacggaa cagtaaaatg
     7021 gttcaacgct gaaaaaggat ttggatttat ctcacgtgaa gatggaagcg acgtattcgt
     7081 tcacttctca gctatccaag gtgacggatt caaaacatta gaagaaggtc aagcagtgac
     7141 tttcgacgtt gaagaatcag accgcggccc acaagctgca aacgttgtaa aagcttaatt
     7201 ttatctgtaa atcaaattga tggtagacat caatttttac gagagaacaa agcgagtaac
     7261 aaaaatacct gccacaagag acaatttctc ttgtggcagg tatt
//
LOCUS       DAXWOO010000140         7158 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.143, whole genome shotgun sequence.
ACCESSION   DAXWOO010000140 DAXWOO010000000
VERSION     DAXWOO010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7158
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.143"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            18..899
                     /locus_tag="ACGE70_002283"
     CDS             18..899
                     /locus_tag="ACGE70_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFF8693892.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            912..2522
                     /locus_tag="ACGE70_002284"
     CDS             912..2522
                     /locus_tag="ACGE70_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8693893.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAKGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2599..3258)
                     /locus_tag="ACGE70_002285"
     CDS             complement(2599..3258)
                     /locus_tag="ACGE70_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFF8693894.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3541..4206)
                     /locus_tag="ACGE70_002286"
     CDS             complement(3541..4206)
                     /locus_tag="ACGE70_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFF8693895.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(4353..4958)
                     /gene="dhaL"
                     /locus_tag="ACGE70_002287"
     CDS             complement(4353..4958)
                     /gene="dhaL"
                     /locus_tag="ACGE70_002287"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFF8693896.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(4986..5996)
                     /gene="dhaK"
                     /locus_tag="ACGE70_002288"
     CDS             complement(4986..5996)
                     /gene="dhaK"
                     /locus_tag="ACGE70_002288"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFF8693897.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(5971..6366)
                     /gene="dhaM"
                     /locus_tag="ACGE70_002289"
     CDS             complement(5971..6366)
                     /gene="dhaM"
                     /locus_tag="ACGE70_002289"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFF8693898.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            6520..6840
                     /locus_tag="ACGE70_002290"
     CDS             6520..6840
                     /locus_tag="ACGE70_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFF8693899.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(6952..>7158)
                     /locus_tag="ACGE70_002291"
     CDS             complement(6952..>7158)
                     /locus_tag="ACGE70_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFF8693900.1"
                     /translation="IKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTA
                     SVSYSLAGAGVLVAAAYGIIRKKK"
BASE COUNT         1880 a         1508 c         1390 g         2380 t
ORIGIN      
        1 aaaggagtgg ggtttttatg gcagaaatcg tcaaagtcac acatttgaaa aagagctttg
       61 gtcgtttcga agctttaaaa gatgtctctt tcaccgtaca ttcaggtgaa gtcgttggat
      121 ttatcggacc aaatggttct ggaaaatcca cgacgattcg gacactttta gggattataa
      181 aaaaagacgg cggaacggca acgatttttg ggcaagatgt atgggaagat agtttatcta
      241 tccatgaacg tctttcttat gttccaggcg atatctcttt gtggggaaat ttgactggag
      301 gagcgatcat cgatctcttc atgaagcttc atggaaaagg gagtgctcaa aaaaaagatg
      361 aactgattca acgtttctct cttgatccta agaaaaaagc aaaaaactat tcaaaaggaa
      421 atcgccaaaa agtgggcttg attgctgctt tggctgttga ttcggatctc tatttatttg
      481 atgagcctac ttctggttta gatcctttga tggaaacgat tttccaagaa gaagttgaaa
      541 aattgaaaga acagggcaaa tccatcctac tttcttctca tattctcagt gaagtcgagc
      601 gactagcgga taaagtcgtg atcatacgcg aaggaacact cgatgaagaa ggaacactcg
      661 atgaattgcg tcatttgact cgttcgacca tcactataga aacagaagca gagaatagtc
      721 acttagcaca attgaacggt gtccatgact ttgtgcaaaa aggtcacctc gctcaatttg
      781 ctgctgataa cgaagctttc gatgtgattt tagctgaagc gtccaaatta ggtgtgaaaa
      841 aatttgaatc tattccaccg actcttgagg atttattcat gagacattat gaaggatgag
      901 gaggaagcga aatgactgat aaattttcac gctggtccac ccttttttct caaaacctta
      961 gccgtgactg gaaaaagatc ctgatatggc tagtcggtgt cactgctttt tctggtggtt
     1021 ttgtccctgc gtttgaagaa ttgacttccg gacaaggatt agctggatta tacgaaacct
     1081 tgcaaaaccc ggcaatgatt gcaatggtcg gaccaactcc agcttcgaat gctgctgagt
     1141 atactttagg agcgatgtat gcaaatgaaa tgttgctttt ttgcagtttg tttgctgctg
     1201 tgatctctgt tttacacgtg gtgagccaaa cgagaaaaaa agaagaactt ggtctgattg
     1261 aacttattcg ttcctttcga gtcggacgtc aagcaaactc tttagctgtc atcgctgaaa
     1321 gtattctgat caatttgctt ttagcaatat cgatcagctg tacgatgatt tttttccaag
     1381 cggattcgat cactgcaaaa ggtgcttggc tttttgggct atctgtaggt agtatgggga
     1441 ttttggggag tgccgttgca ctgtttgctg cacaactgat gccaagttca gccagttcga
     1501 atggtctgtc tttagcgttt ttaggtggac tttaccttct acgagcaata acagatgtag
     1561 ctgattcctc cctttcgatg tggaatcctt taggctggtc ttatttgacc tatccattta
     1621 caactaacaa ctggattcct ttgctttatt tgtttttgtt cagtctaatc ttgataatca
     1681 gttcatttgt tttagaggaa catcgagatt tgaacaccgg ctatatcccc gaacgtgaag
     1741 gaaaaccgta tgctaaacgt tctcttttat ccttgcgtgg tttattattc tatctaaata
     1801 aagggatgac aatcagttgg cttatcactt ttggtttgat gggggcggca tacggctcta
     1861 tctacgggaa tatgcaacag tttttagaaa gtaacgactt aatgaaacag atgtttatcc
     1921 aatctggcgt ctcaattgaa aaaaacttta caagcacgat catggtagtc ttgatttgtc
     1981 tagctgctat tttaccaatt gctagcatca atcgtttatt tacagaagaa acccacggac
     2041 gtatgaatca gctgttcgca acaaaaattt ctaggaacca actttattgg accaatatgt
     2101 tactagctat tctatttgct atattggcaa tcctgattag ttctggtgca ctagggcttt
     2161 ctgctttggc ttcgatgaag gatgcgaaag acatggatct attgatgttt cttaaagccg
     2221 ggttcaattt cctgcctgcc gttttagtgt ttatcggaat tagtggcttt ctattcggat
     2281 ggcttcctcg aatcggtaaa cttgcttata tctacttagc ttactccttt gcactcaatt
     2341 actttggcgg aattttggat ttaccagaat ggttttccaa aacagctgtc cctagctggg
     2401 ttccacggat gccaaacgat tcttttgatc tttccgtgtt ccttacgtta tcatgtatca
     2461 gccttgcact gttcgtcctt ggttctattg gttataggcg aagagactta ctagaaggat
     2521 aataaaaaag catgtacaat ttcatgctag cctccaaatc tgggtcaaaa atccaacatt
     2581 tggaggtcat ttttttattt acaagtcttc taactgctct ttatcaatga tccagacttc
     2641 tcgattcttt ctttttacat aaccattctc ctctaataat ttaaattttc tagataaagt
     2701 ttcgggggtc gtaccgataa atgcagcaag ttctttcatt ttcattggca ggaaaaagtg
     2761 atccttccct gtcgctttag ctaagtcaag cagatagaca gcgactcgtt cttctacttt
     2821 ttccatcatc aaaaaagttg tctgctgctg tacttttgtt aatttctcaa cgttgattgc
     2881 taaaagtttc agactaaggg cgggataatg cagtaagagt tccgagaaat ctttttgtct
     2941 caatatgcaa atttccgttt tttctagtgc ttcgccaaat aaagactcat ttggaacgcc
     3001 tagcaactgg ctctctccct catatccgcc cggctctatt actcgtaaaa gctgttcctt
     3061 tccgctagca gataattgat acactttcat actgccggag gccacgatca ctaattgagg
     3121 ttctccattc ggcgcaagaa tctgttctcc tttttccaca tagtgatgtt tgacaagtgc
     3181 gtgtactttt tcttgatctt gctgtactaa gtgattgaac aaaggaacaa atgaaacaca
     3241 taaatgatca tgactcataa tctctcctcc ttctaaacag ctatttctaa taaagaaaca
     3301 taacttccct tattccaact gataattttc ttttatcata gcataaacgt caagcgaacc
     3361 agataccagc aaatagagtg gatttctttt tgtcccttgc tttattttcc ttcttttgag
     3421 accttaagta attgattcct atcaattttt taaatattta ctttttgaat aatcaaaaac
     3481 aaaccttcac tcgcaagccg agagtaaggt ttgtttttcg cattgcttat ttcgtgttgt
     3541 ctattgaaat ttttccgcac ctagcttttg atatagtggt gctagttctt gtaaggtttc
     3601 agcggttatt tgctcaacgt gttcgatttc ttgcggatag aagaatagtt tacccatttg
     3661 aaaggttcgg tctctcatcg cttttttcaa ctcttcttgt ttctttgtat cggacaaata
     3721 gtcagacaaa gctaagtggt cgtcaagttc atgttctggt tgcggccata tgtaataatc
     3781 cttgatatcg actgttttgc tccactcttt cagtatcgca agcaactggt tatacgctgc
     3841 aatgctacca tatcgttctt cgcttttata atgttggtac atcaaataga tttggtattg
     3901 tttttgattc aacaaaactg ctagacaggc attctcgtct tgacgagatt cgcttcggta
     3961 cgcgctccaa aaatgactgc gtaaattcca gccattcgtc cagctttcga ttttaggttt
     4021 gctcatgccg tacatatcag gcaaaagtgc agctgtctgg agctgaatct ctttccattt
     4081 ttgccagtgt tctttatatt ctttcttgat ttgttcaatc ttatctgttg gatggctctc
     4141 tttgagctgt ttgaatgcgt agattggtcg atcaaacaac tgataatctg attttccaag
     4201 ttccattgtt tcgcctcttt ccgttatctt ttgtattcta cctgaatttt gcgatatgga
     4261 caaatagagg ttgtgaaaaa gctgttctgc atcaagtcat aagaacagcc aaacaaaaaa
     4321 ctgcgatgag catcaccgca gttttattga acttaaggtt gattgatcac atctaaaagc
     4381 gttgaaaata aaatggcact agaagcagct cctgggtcga tatgtccgat tgcacgctct
     4441 cctagatagg aagctcgtcc ttttttagct accaagactt tggtgtgttc ttttgcctgg
     4501 tcaatgattt cttttgttaa attccctgcc ttcaatgcct ctgtgactgg cagccaaaca
     4561 tctaacatcg ttttatcgtc agcttctgcc tttcctcggc tttgaatacc cgctgttccc
     4621 tgctcgatca cttcgcctaa tttttcttga gaatcaatag aatcgacctc ttttgtcgct
     4681 ttcatcatgt tcatgaatgc agatccgtat aacggaccag aagccccacc gactttcgat
     4741 atcatcgcca tagagaatac tttgaatgta tcgctgattg tttcaggatg atcggcttga
     4801 aaagcttctt cataagctga aacccctctt cccatgttat ttccatggtc tccatcgcca
     4861 atcggggtat ctagatcact gagatattgt ttgttctgat tgattgcctc tgcaaattgc
     4921 gtgagccatt cttggatttc ttgaacggtt agtttcatat tatcaccctt ttatcttgag
     4981 attttttacc atgaaatcgt ctgtacattg ctttctaaag aatctttcca ttgtgtctcc
     5041 gtcaagtcga tcagtgttag agaaagacct tgcatatcta gtgaggtcat aaaattaccg
     5101 acttttcgga aaactacatt gacgcctctt tcttccaaca gagctaaaac atcattcatg
     5161 aacacgaact gttccatcaa aggcgtcccg cccattccgt tgactagtac acctgcttct
     5221 tttggtaaat tcgagtaatc atctaaaatc ttagtaacaa gttcctttgc cagtacttta
     5281 gaaggctgca tcttttctcg acgatagcct ggctcaccgt ggataccaac accatactcg
     5341 atctcatctt ctcctagttc gaatcctggt ttccctactt ctggtacagt tgctgctcga
     5401 agcgctagac cgatcgtctt agttgcctgt acgacctttt ctcccagttc tttcaactca
     5461 gctagggaag caccgtttct tgctgcatcg cccacgattt tgtgtacaag gacagtacct
     5521 gcaactccgc gttttccagc agtatacgta ctattttcaa cggcgatatc atcatctacg
     5581 atcacacttt ctacttcaat atcgtccata gcagctaatt cctttgccat atcgaagttc
     5641 aatgcatccc ctgtataatt cttaacgatc agcaaaactc cttttccact atcggcttcc
     5701 ttaatagccg tctgaatctg atctggagtt ggagaagtaa acacatcacc aagcaccgct
     5761 gcacttaaca tcccttcccc tacaaacccg gcatgggatg gttcatgtcc gcttcctcca
     5821 ccagaaacca aaccaacttg ttccgctttc tgattcttgg ctacaacacg ggaagattct
     5881 acacgatgaa caagttctgg atagcttttt actaatcctt ccagcatttc ttcgacaata
     5941 ttgcttggat cattcatgat ttttttcaat ttattcccat cctttctttc cggcattttg
     6001 tgcttctgtt aagatttgct gagtatcatc tgttataccg gcagtgatgg ctgctgcaaa
     6061 agctccttcc actaaaggcg catccaccag atgataacgt tgctgctgct cttcttccaa
     6121 catgtctttt gccagttccg agttcaatac cgcgctaccg atatccgcaa aaacaaaata
     6181 actagctgca tcgcttgatt cattgactgc ttctacgatt ttcatcgggt ctgatccgat
     6241 ctcgccattt attccgccga gagaaaaaat ttgcacctct tcggattttt gcatctgctc
     6301 aatcatttct ttaataccat ctgttacttt ttgactatga gaaataagca aaatactttt
     6361 tttcacaaga atcttccttt cttttacata gttgctaacg attggtttcg ctttcattaa
     6421 tgaacaattc aagtatataa taaatttcta attttgggaa aatatgtgta gtgcttcttg
     6481 aagtttgtta tactttaatt aaaagagagg agtgatcgaa tgattacaac aacaacgaac
     6541 agtgtagagg gaaaagccgt agtagcttat aaagggatcg tttttggtga ggtcattact
     6601 gggatccatg catttaaaga cttggaggcg gggttgcgaa atatctttgg cggccgctca
     6661 aaaagctatg aaaacgaact aatgggtgca agagaagaag cacttgccga aatggccgaa
     6721 cgtgcaaaag cattaggagc tgatgcgatc attggcgtaa aaatggacta tgaagtatta
     6781 ggcagcgaca atggcatgct gatggtcaca tgtagtggaa cagctgtctc cacgagataa
     6841 gtaggggaca tctgttttaa taactaaaga gagatgggac atgtagtggc actcaagaat
     6901 tagatttttc agtctaactt cgaggtactc taccttccca tcctctcttt tttatttctt
     6961 tttgcggatg attccgtaag ctgctgcgac taacacaccc gcccctgcta aggaataact
     7021 gacagaagct gttttttcat ttgtttttgg caaagtctga cttgttgatt tggatggctc
     7081 tgtcgtttgc tgaactgttt ctgtcgtgct tccagaggtc actgattttt ctcctgccga
     7141 atgcaaaata tcttttat
//
LOCUS       DAXWOO010000141         7144 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.144, whole genome shotgun sequence.
ACCESSION   DAXWOO010000141 DAXWOO010000000
VERSION     DAXWOO010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7144
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.144"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(<1..2777)
                     /locus_tag="ACGE70_002292"
     CDS             complement(<1..2777)
                     /locus_tag="ACGE70_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen binding domain-containing protein"
                     /protein_id="HFF8693901.1"
                     /translation="MKKLKLISVALFSLITLSTARQSVVSAEGTSTKAVVEESGDKKV
                     AIDEKVKNEAEKATVVQGADGLMYVVVDENNPEKLLEVESPLKNQQENTGAVEVGVDE
                     KRQEKADKANEKVKNFDDIDYNDISSLQSALNDPKFPFLSEDELNEKLQKTKNTFETE
                     ALMKKTRGYFSQRILELSNNLNDVPKRAKVSRRALVSNRASQVSETRINPPNGYPWVI
                     YLIRSNGYSYTENYAIRRTIDGKEAFCLQAGVQATNTYTGSTSGYLSNSQREMISSIL
                     SAYKELGGWANNDAGKQRWIATQMLVWENSNEKPKSITVTPNWKNGATKWKNDLVARA
                     NAIRVRTKWDSTSKTVDEGDKATFKVTNTYGDHNLHVKSVSGGGKATISNGTLTVDTT
                     GATGNKITVDMIKGSHPSDAHPTTVWSNGSYQKLVTGSFYISTSVTVNVKRKGTVEVL
                     KVDSETKKPLSGATFRFSYSGKTKDVTTDSNGKAKLTERLKAGTVVKVKEIKAPNGYQ
                     LDSSEFSITVKENQNVTTTRTNKKSTGSVEIEKIGDLDGLGLPNVIFTIYSSDNKVVK
                     DNLKTDSNGKIKVDELQFGKYYAVEKQGVTGYDPDGKKYNFEITQDTPSTKPAKVKVT
                     NIYEVSVSEKVSDMNESQVLKNVWSADPSDYLQYDITTGNLKKSGERNVQSFTIEGFY
                     DNKNVDVSKSEVYVGSTNVTSSFDISNDTGNGKVVARAKATVLTNNDFYNKSYNLRIT
                     MKIKKSTLENTEKQKPLTIVKNSKVTVGSKISKSANSNDVETVLYSRKITINHIDEKD
                     KHLLKQDIDYKYDGETYEYKPRTDLFDKDGNNYKSDLIHKGKIDGEDIVLTTPYHIPV
                     LSVNVDRIQIDTARAVKNGYLPTELVLSKKEEYEKELEKIVFKVKITDIDNNKVVYDE
                     NFKYKEFNSHMS"
     gene            complement(3195..3518)
                     /locus_tag="ACGE70_002293"
     CDS             complement(3195..3518)
                     /locus_tag="ACGE70_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693902.1"
                     /translation="MARSRIAKIEQTKKDQKISKNKTKFFKAEDVKRIAWSVKTFDED
                     RYVKVSMNGLFVQLGNFSNFKTIDELYPEIRNIVDSKGLIVAKRKSRNSGLAYCKDKG
                     IMIPA"
     gene            complement(3663..4106)
                     /locus_tag="ACGE70_002294"
     CDS             complement(3663..4106)
                     /locus_tag="ACGE70_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFF8693903.1"
                     /translation="MQILEDIKIYWKRIIGYTLVLLVVNLFLSMIALVFVNQRKDNVT
                     ELVTFISFFISGIFAPFFEETLMRGIIQNQLELKTNLSTRSIYIIVAILFSLFHLNLY
                     FIPYFMTSLILSFVYDNSDKRLIVPVTIHCFYNLIVILLSIVFYT"
     gene            complement(4109..4408)
                     /locus_tag="ACGE70_002295"
     CDS             complement(4109..4408)
                     /locus_tag="ACGE70_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693904.1"
                     /translation="MDHKSNYSYKENYIWKYVVAVFLLVGGIIALVKWAIIPNALVFI
                     NWFVPNFWSFFGYILIIWFMFKVLSFFWSPVYSLLGKWSYLILIATAIYIIMQIN"
     gene            complement(4426..4860)
                     /locus_tag="ACGE70_002296"
     CDS             complement(4426..4860)
                     /locus_tag="ACGE70_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693905.1"
                     /translation="MSKTKKVTSAGSNGSGNPFGIKSGSGGFNIGQQMLKGKAGIKST
                     GDKRKSVRVPVELYDEVVKDLSYGAKYSLMTEILALFLKEFETHGNSIVTDFGKLKTR
                     PVKISEEAFKVLKVIKFETDQSNTNIIASALTWYLDKRKGKA"
     gene            complement(4876..5727)
                     /locus_tag="ACGE70_002297"
     CDS             complement(4876..5727)
                     /locus_tag="ACGE70_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFF8693906.1"
                     /translation="MKSKFLGYVIAINMFKGGVGKSTLTQLMADILSARGFKVLVIDV
                     DPQGTVTKKFQRQYDLKVVGKMTLFEAVINMSFLDCISPVKENLDIVLGSEQMSRFTK
                     VLRDMWRSTGFTDEGEEVFWNLFNYMIGNDNLRDQYDFILFDTIPTVSEFTDNVYVAS
                     DFLVIPTQTEQDSIDNLHSTVTNYQTAVKEVNGDLEILGIVPYLVDTKLKTGLELLSE
                     LQEEYGDLVYSHIVSERGVIKRWGREGLNKSIPHSKTAYSMYVDIVEETLVRLEKATG
                     ENILERV"
     gene            complement(5810..6016)
                     /locus_tag="ACGE70_002298"
     CDS             complement(5810..6016)
                     /locus_tag="ACGE70_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693907.1"
                     /translation="MIENRVLGYVECKDNLDDTLEVLESIIFNTKVLDLKAINGDMTT
                     HIILDEISAKEIGETLIAWANKEL"
     gene            complement(6399..6623)
                     /locus_tag="ACGE70_002299"
     CDS             complement(6399..6623)
                     /locus_tag="ACGE70_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693908.1"
                     /translation="MISMARPRKYNTEEERKEAMKKARKKWVEKNQETIAYIRAKSFA
                     KKFIEMSEDEDIELLEKWLEDRKNNGQNDE"
     gene            complement(6735..>7144)
                     /locus_tag="ACGE70_002300"
     CDS             complement(6735..>7144)
                     /locus_tag="ACGE70_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type III toxin-antitoxin system ToxN/AbiQ family
                     toxin"
                     /protein_id="HFF8693909.1"
                     /translation="KENQRSLTNKSVFKIYGDREEKDFLGVLHINNMIPVPKDCAKVW
                     TPNSPNVDEKYKMLVIKQAKYLKLHESEINESCELLYNSKLGKLDPSYFKKNRKEVIT
                     YQKIMTNVESLEAVSIAESQRNIHVQTQSIDVE"
BASE COUNT         2200 a         1363 c          881 g         2700 t
ORIGIN      
        1 gacatatgac tattaaattc tttgtatttg aagttttcat cataaacaac tttattgtta
       61 tctatatctg taattttcac tttaaataca attttttcta actctttttc atattcttct
      121 tttttgctta gcaccaattc tgtaggtaaa tagccatttt taacagctct agccgtatca
      181 atttgaatcc gatccacatt cacagaaagc accggaatat gataaggagt ggttaaaact
      241 atatcttctc catcaatttt ccctttatga attaaatcag atttataatt atttccatct
      301 ttatcaaaca aatctgttct aggtttatat tcatatgttt caccatcgta tttataatca
      361 atatcttgtt tcaaaaggtg tttatctttt tcatcgatat gattgatagt aatttttctg
      421 gaatataaaa ctgtctccac atcattagag ttagcggatt tagatatttt tgaaccgaca
      481 gtaactttac tatttttaac aatcgttaag ggtttttgct tttcagtgtt ttcaagcgta
      541 gattttttta ttttcatagt gatccgtaaa ttataggact tattataaaa atcattattc
      601 gttaacacgg ttgccttcgc cctagcgact acttttccgt tgccagtatc gttactaata
      661 tcaaaagagc ttgttacatt tgttgatcca acgtatactt cactttttga aacatctaca
      721 tttttattat cgtaaaaacc ttcgatagta aagctttgga cgtttctttc tccggatttt
      781 tttagatttc ccgttgtaat gtcatactga agataatcgc ttggatcagc agaccaaaca
      841 ttttttaata cctgactttc attcatatcc gatacttttt cgcttacaga tacttcataa
      901 atattggtta ctttcacttt agccggtttt gtggaaggtg tatcttgggt tatttcaaaa
      961 ttgtattttt ttccatcggg atcataacca gtaacacctt gcttttctac tgcataatat
     1021 ttaccaaatt gtagttcatc aacttttatt ttcccattac tatctgtttt taagttgtct
     1081 tttacaactt tattatcgga actataaatc gtgaatatta cattcggcaa tcctaatcca
     1141 tctaaatcgc ctattttctc aatttctaca gaacctgtgg atttcttatt ggttcttgtg
     1201 gtagtaacat tttgattttc tttaactgta attgaaaatt cgcttgaatc tagttggtag
     1261 ccattaggag cttttatctc cttaactttt actacagttc cagctttcaa cctttcagtt
     1321 aattttgctt taccattcga atcagtagta acatcttttg ttttacctga gtagctaaat
     1381 ctaaatgttg ctcctgataa tggttttttt gtttctgaat caacttttaa aacttcaaca
     1441 gttccttttc tcttaacatt taccgtaaca gaagtactaa tgtaaaaaga accggtaaca
     1501 agcttttgat aagaaccatt actccatacc gttgtagggt gcgcgtctga agggtgagaa
     1561 cctttaatca tatctacagt gattttgttt ccagtggctc ctgtagtatc aacagttaaa
     1621 gtaccattgc ttatggtagc tttgccgcca ccagatacag atttcacatg caaattatga
     1681 tctccataag tattagttac tttaaaggtt gctttgtctc cctcatcaac cgtcttagac
     1741 gtagaatccc attttgttct tactctaata gcatttgcac gagctactaa atcatttttc
     1801 cacttagtag ctccgttttt ccaatttggc gtaacagtta tacttttagg cttttcatta
     1861 ctgttttccc aaacaagcat ttgagttgct atccatcttt gttttccggc atcattatta
     1921 gcccaaccac ctaattcttt atatgctgat aaaatcgaac taatcatttc tctttgcgaa
     1981 ttagaaagat aaccacttgt tgaaccagtg tatgtattcg tcgcttgtac accggcctgt
     2041 aaacaaaaag cttctttccc atcaatagtt cttctaatag cataattttc tgtataacta
     2101 taaccattgc tcctaattaa ataaatcacc cacggataac catttggagg gtttattcgt
     2161 gtttcactta cttgtgaagc acggttacta actaaagcac gccgactcac tttcgctctt
     2221 ttcggtacgt catttaaatt attgcttaac tctaaaatcc tttgactgaa atatcccctt
     2281 gtctttttca ttaacgcttc agtctcaaat gtattctttg tcttttgcaa tttttcattt
     2341 aattcatctt ccgataaaaa agggaatttc ggatcattca acgctgactg caacgaacta
     2401 atatcgttgt aatcaatatc gtcaaagttt ttcacttttt cgttagcttt atcagccttt
     2461 tcttgtctct tttcatcaac accgacttca acagcgccgg tattttcttg ttgatttttc
     2521 aaaggagatt caacttctag caatttttca ggattatttt catcaacgac cacatacatc
     2581 aagccgtctg caccttgaac aacagttgct ttctccgctt cgtttttcac tttttcgtct
     2641 atagcaactt ttttatcacc agactcttct actactgctt tcgtagacgt tccttcagcc
     2701 gaaacaactg attggcgagc cgttgataaa gtgattaggc tgaatagagc aacgctaatc
     2761 agtttcagtt ttttcattat cacacttcct ttagaaagaa atgtaataaa gaatcataac
     2821 aactctttac tacatttctt gtagtaaaga gttaccctgt gtctagcaca tttattactg
     2881 tgctgaattt tttagtgtca atattctgat ctctaggacg attttcatag gttcttccta
     2941 gatagaaact ttgaataatt accatttacg ccacctcata attagtagtt tcaatggatc
     3001 gacttttgta ttttttggcg tgaaatacta accttatcat ttctatcgct aacgtattta
     3061 catacctctt ggcaaaggaa ataacagtat gcttggttgc tattttgctt gttagcccta
     3121 cgcttttatc tgtaacatag caacattgct acagtacgag tagtattaag ttttcaagga
     3181 acaaattcac tgatttaggc tggaatcatg attcccttat ctttacagta agcaagacca
     3241 gagtttcttg atttccgttt agccacgatt aaacctttac tatccacaat atttctaatt
     3301 tccggataaa gttcgtcaat cgttttaaaa ttactgaagt taccaagctg tacaaacaga
     3361 ccattcattg aaactttaac ataacgatct tcatcaaatg ttttcacaga ccatgctatc
     3421 ctcttaacat cttcagcttt aaagaatttt gttttatttt tagaaatttt ttgatccttt
     3481 ttagtctgtt caattttagc aattcttgat cttgccaaca gctatcactc ctttaatttt
     3541 tttactaaat cagttcatac ttaattatat catttatgac aatttctatc aacctgatag
     3601 ataaaaacaa aacaaaaaaa atagataaaa accttgtatt tttaggtttt cgtctatttt
     3661 tttcaagtat aaaaaactat acttaacagt attacaatta aattgtaaaa acaatgaata
     3721 gtaaccggaa caatcagcct tttatctgaa ttatcataga caaaagacaa aattaaactc
     3781 gtcataaaat aaggaataaa atataaattt aagtggaata gactaaacaa aattgcaacg
     3841 attatatata tgcttcttgt agataaattt gtttttaatt ccaattggtt ttggataatc
     3901 cctctcatta aggtttcttc aaagaaagga gcaaaaatcc cacttatgaa aaacgaaata
     3961 aacgttacta attcagttac attgtctttt ctttgattaa caaagaccaa cgctatcatc
     4021 gaaaggaata aatttactac aagaagaact aatgtatagc ctattatcct tttccaataa
     4081 attttaatat cttctaaaat ctgcataatt agttaatctg cataatgata taaatagctg
     4141 tcgctattaa aattaggtac gaccactttc caagcaaaga atatacaggc gaccagaaaa
     4201 aagagagtac tttaaacata aaccagatta tcaatatata gccaaaaaaa ctccaaaaat
     4261 tagggacaaa ccaattgata aatactaaag cattaggaat tatagcccat ttaaccaatg
     4321 caataattcc acctaccagt agaaaaacgg ctactacata cttccaaata taattttctt
     4381 tgtatgaata atttgattta tgatccatag tcttacctca tttcattatg cttttccttt
     4441 tcttttatca agataccatg tcaaagctga agcaataata ttagtattag attgatctgt
     4501 ttcaaatttt ataactttca aaactttaaa ggcttcttca gaaattttta ctggtctcgt
     4561 tttcaacttg ccaaaatcag taactatact atttccgtgt gtttcaaatt cttttaaaaa
     4621 taaagctaaa atttctgtca ttaatgaata tttagcaccg tatgataaat ctttaacaac
     4681 ttcatcgtat aactcaactg gtaccctaac agatttccgt ttatcaccag tagacttaat
     4741 gccagcttta ccttttaaca tttgttgtcc tatattaaat ccaccactac cgctttttat
     4801 tccgaacgga ttacccgaac cattactgcc ggcactagtt acttttttag ttttcgacat
     4861 catgcaacac ctttcctaaa cacgttctaa aatattttct cctgtagctt tttctaaacg
     4921 tactaacgtt tcttctacaa tgtctacata catgctataa gcagttttag aatgtgggat
     4981 agatttattt agtccttctc taccccaacg tttaattacg cctcgttctg aaacaatatg
     5041 agaataaact aagtctccat attcttcttg aagttctgat aaaagttcta atcctgtttt
     5101 taatttagta tccactagat atggaacaat acctaaaatt tctaaatctc cgtttacttc
     5161 ttttacagca gtttgataat tagtaaccgt actgtgcaag ttgtctatcg aatcttgctc
     5221 agtttgcgtg ggaattacta aaaaatcact cgccacgtaa acgttatctg taaattctga
     5281 aactgtagga atagtgtcaa acaaaataaa atcatattga tctcttaaat tatcattacc
     5341 gatcatataa ttaaacaaat tccaaaagac ttcttcacct tcatcagtaa aaccagtact
     5401 tctccacata tctcttaata ctttggtaaa ccttgacatt tgttctgaac caagcacaat
     5461 atctaagttt tctttaacag gagaaatgca gtctaagaaa gacatattaa ttactgcttc
     5521 aaacaatgtc attttcccaa caacttttaa atcatattgt ctttgaaact ttttagttac
     5581 tgttccttgt ggatcaacat caataactaa aactttaaag cctctagccg atagaatatc
     5641 agccattagc tgagtaagag ttgacttacc aactccccct ttaaacatgt tgattgcaat
     5701 tacatatccc aaaaactttg atttcatcat gtatacctca cttttgataa tttattatac
     5761 tatacagact acttgtagtc aattggccac tttattttaa aattatcttt tacagctctt
     5821 tattagccca tgctattaaa gtttcgccaa tctcttttgc gctaatttca tctaatatga
     5881 tatgtgttgt catatcacca ttaattgctt tcaaatccag aactttggtg ttaaatataa
     5941 tactttcaag gacttctaaa gtatcgtcta aattatcttt acactctacg taccccagta
     6001 ctctattctc tatcaacttt agctcctcct tataatcaga taacaaaaaa attctctaaa
     6061 agtcctacaa aaaaaatcta gtaaaaaagg tcaaaagtga acgattcatt aatataattc
     6121 gttattttcc attatgagcg gttttgtaat aatttcaata tttttttcta cgcttgaaca
     6181 atacatagat attaagaaaa tatttagtga aatttatcaa cgctcattta ataaatcgtt
     6241 cctcaaaata ttcaaattcc tcctttcttt aggccataat cgtctgtttt tattcccgat
     6301 ccctcaattg actacttgta gtcattagac ttttagaaaa gggatatttc tatccctaaa
     6361 accattctac accattgact acttgtagtc aatggttatt attcatcatt ttgaccgtta
     6421 tttttccgat cttctaacca tttttccaac aattcaatat cttcatcttc agacatttca
     6481 atgaattttt tagcaaatga tttggctctt atataggcaa tagtttcctg atttttttca
     6541 acccattttt tcctagcctt tttcattgct tcttttcttt cttcttctgt attatatttt
     6601 ctcggtctcg ccatagaaat catcctttaa gttttatctt ctacagtata ataatattta
     6661 aatacatatt cgattaaaat tcaatgtatc ataatatcca atagaataat aaaataagat
     6721 aggtttagcc tatcttactc tacatctata ctttgagttt gtacatgaat atttctttgg
     6781 gattctgcta tactaacagc ttccaatgat tctacattcg tcattatttt ttgataagta
     6841 attacttctt tacgattttt cttaaagtaa ctaggatcaa gtttacctaa cttagaatta
     6901 taaagtaatt cgcatgattc atttatttca gactcatgta atttcaaata ttttgcctgt
     6961 ttgattacta acatcttata cttctcatca acatttgggc tattaggtgt ccatactttt
     7021 gcacaatctt ttggcacagg aatcatatta tttatatgaa gtactcctaa aaagtccttt
     7081 tcttctcgat caccataaat cttaaacact gatttattag tcaaagaacg ttgattttct
     7141 tttg
//
LOCUS       DAXWOO010000142         7036 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.145, whole genome shotgun sequence.
ACCESSION   DAXWOO010000142 DAXWOO010000000
VERSION     DAXWOO010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7036
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.145"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            177..1034
                     /locus_tag="ACGE70_002301"
     CDS             177..1034
                     /locus_tag="ACGE70_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFF8693910.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            1027..1929
                     /locus_tag="ACGE70_002302"
     CDS             1027..1929
                     /locus_tag="ACGE70_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFF8693911.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            1962..2312
                     /locus_tag="ACGE70_002303"
     CDS             1962..2312
                     /locus_tag="ACGE70_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFF8693912.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            2361..3284
                     /locus_tag="ACGE70_002304"
     CDS             2361..3284
                     /locus_tag="ACGE70_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFF8693913.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            3362..4255
                     /locus_tag="ACGE70_002305"
     CDS             3362..4255
                     /locus_tag="ACGE70_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFF8693914.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            4293..4856
                     /gene="amaP"
                     /locus_tag="ACGE70_002306"
     CDS             4293..4856
                     /gene="amaP"
                     /locus_tag="ACGE70_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFF8693915.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            4870..5058
                     /locus_tag="ACGE70_002307"
     CDS             4870..5058
                     /locus_tag="ACGE70_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFF8693916.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            5071..5973
                     /locus_tag="ACGE70_002308"
     CDS             5071..5973
                     /locus_tag="ACGE70_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFF8693917.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            6005..6247
                     /locus_tag="ACGE70_002309"
     CDS             6005..6247
                     /locus_tag="ACGE70_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFF8693918.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(6503..6874)
                     /locus_tag="ACGE70_002310"
     CDS             complement(6503..6874)
                     /locus_tag="ACGE70_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693919.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         2569 a         1194 c         1395 g         1878 t
ORIGIN      
        1 acctttgaat aaaaagccat gaaaaacgaa tagttagcgc ttaccgatgc gttttgtgat
       61 aaaaaaattt taaaatattc aatgaaaaca aagcatatat tttgctttaa aaccaaatga
      121 ggaatattct agatgtagaa caggttagaa aaagattaga aaaaggagtg attttgatgg
      181 agaagagata cggcgtagtt cctgtggtaa ctgcttcaga cgaaaattat gctccttact
      241 tgagcgtgat gatcgcaact gcattagaaa attgcaataa aacaagacgc ataaaatttt
      301 atgtgattga cgatggtcta tcagaatata gtaaagaagg attggaagaa acggtaaata
      361 aatattcaag taatgcaagt atccaattcc tgacagtgga aaaagatatc tacgaagact
      421 tcctagtcag tgaccatatc acaactaccg cttacttaag aatttcttta ccaaacttat
      481 tagctaaaga ggattataag aaagtccttt acttagattc agatgtgtta gtactagacg
      541 atatcgtaaa actatatgat gagccgttaa atggaaaaac aatcggcgct attatcgatc
      601 caggccaagt gaaagctttg gaaagattag gaatcgattc cgatgacttg tacttcaatt
      661 caggagtgat ggtgattgac attgatcaat ggaataaaaa agaaattaca gaaaaaacta
      721 tccattacct cagtgaaaat ggcgatcgaa tcatctatca cgaccaagat gctttgaatg
      781 ctgtacttta tgaagattgg gaacaacttc atccaaaatg gaacatgcag acttcgctga
      841 tttttgaacg tcatccagca ccaaatgaaa aatatgaacg tcagtataaa gaaggaaatg
      901 aaaaaccttc gatcgttcac tttacaggac atgataaacc ttggaatacg ttgaaagatc
      961 atccgtatac caatttatac ttaaaaaaat tagcacacag tactctaacg aaagtaggcg
     1021 agatcaatga ataaaaaaga aatagcagta gtcgcaagtt gtaacacaaa attcgtacca
     1081 catttagctg cattatttgt ctcagtatta gataattgca acccgtctaa atttgttcgg
     1141 ttctatgtga ttgacgatga tatcgatttt gaaagtaaac agcttttacg cttctcagtg
     1201 aaaaacgcac gaatgaactc agatgtggaa ttcttgaaaa tcaataaaga attctttaca
     1261 aatgtggtaa tcagcgatcg tattccagaa acagcttatt atcgtattgc gattccggaa
     1321 ttgttccgcg gcacagaagt tgaacgtatc ttatatatgg attgcgacat gatcgcttta
     1381 caagatatct ctaaattatg gcgtcttgac ttcggtgatt caatcgtagc agctgttgaa
     1441 gacgctgggt tccatcaacg actggaaaaa atgaaaattt cagctaaatc aatgcgttat
     1501 ttcaactcag gtttgatgct gatcaatgtg aaaaaatggt tagatgaaaa tattacgcaa
     1561 aaagtattag atttcatcga acataatcca gaaaaattac gattccatga ccaagatgca
     1621 ttaaatgcta tcttacatga ccgttggtta ccacttcatc cgcgttggaa tgcacaaggc
     1681 tatatcatgg caaaagcaaa aaaacatcca acagcagcag gtgaacgtga atacgaagaa
     1741 actcgcaaca atccttacat tatccatttt tcaggacatg tgaaaccgtg gagcaaggat
     1801 tttgaagggc caacgaagaa atattacgaa aaatatgcag ggatgacggc tttccgttgt
     1861 gtagctaaat tccctaaata tccaaaatat gcaaaagttc aaccaaaaac agatgtagtt
     1921 gttaaataat agaagaccac aactgaagag ggggagtagg aatggaaatt acaatgtcta
     1981 aaaatgcagt agagacgtta attgaaaagg ttggggaaaa tacaaaaatc gcactcgcgc
     2041 ttatcaatga ttccgatcca tttctaaggg acaaaggtgc ttttgcaaaa ggcagctttt
     2101 ttcaaatcat tccttttgtt tcggaatttg gagaatatgc gactaaaatc gagcatccat
     2161 tattggacat ctatacatct aaattagagc aaaattattt cggtaaacga ttgaacatgg
     2221 acttcaacaa acagcttgac agcttttcat tagaaaatga aatcgcagta ctcgattata
     2281 atatcaaatt aaagaactgc ttcttcagct aatagtttcg agaaagtcca aaaaggaggt
     2341 atacattgag atactacttt gtggatgaag aaaagaatgt aatagaaaca gatgaaaaaa
     2401 aatataattg gctcgtactt gatagtgcaa agcccgaaga gattgagcaa gcaatcgaag
     2461 aattcgagct tcctgatgat attttcgtag gaaccgaata tccggaagaa gtttctcgga
     2521 tggagcgcct ttatgagacg aaactagagc atccattttc tttggtagtg attaatctag
     2581 atcagtcaga taatcgtatc gaaaaaaaat taacgccaat atcttttgta ctttccgata
     2641 atctcttgat tacttgtatc gatagaaaaa ccgaatttgt ggatcggctg ctgacaaagc
     2701 atggaaatca tttaaactcg tttgaaaaga tcatcacgta tacattatta gatatttaca
     2761 cccattacgt gaaagaacta agagagatga aaaagcgaat tgacgctttg gataaagcag
     2821 ctcgtaaaac aacggaaaat gaagaactgt ataaacaggc agatcttgaa agagacatcg
     2881 tatatatcga tcatacctta agagatcaac gggaaacaat ggatcaatta tggaattctg
     2941 aaactttcat caaacgttta gacgatgaac agctactcta tgatgtccat ttgagacagc
     3001 gacaaacaga aaaaatgatc gagatctatc gagatttatt agagagtatt ggtgatctat
     3061 tcaacgggat gatggacaat aatctaaatc atatcatgaa atatctagat tcagctgcat
     3121 taatcatttc cgttccggcg ttgttcgcag ggatttgggg aatgaataca ggaggattac
     3181 cgggtaaaag ttctgctcta ggctttatac tcgtagtctt tggctctttg atagcagcag
     3241 tagttttagg ttaccattta taccggaaag attatacaaa ttaaagaatc tatttaaaac
     3301 aggtatcaaa caaagaaatt tgttttggca atacaattta aaaactagga ggaatacagc
     3361 aatgacagaa ccaaaattaa tcgacccgcg aaacttgtac catacggaga aatttcctaa
     3421 acaagatcag gatacaccag ctttacagca gaatatgatt cctgttcctg attgtggaga
     3481 agaatcctat gaaggcaata atcaactaga aaaccgccgt gtactaatca ctggtggaga
     3541 ttcagggatc ggtcgtgcag cagcgattgc atttgctaga gaaggggcag atatcgctct
     3601 tcaattcttc ccaggagaag aaaaagatgc agaggaagta gcaaaatacg tgaaagatgc
     3661 aggacgtaag attgtcttac taccttttga tcttcgtgac gaacaagcac ctaaagaaat
     3721 catagataaa gccgtgagtg aacttggcgg gctagatacg atggtactaa acgcaggaca
     3781 gcaaatttct tgtcagtcta ttgctgagtt accacttgag caagtcaaag acacatttat
     3841 gataaacatc atcgctatgt ttgctttagt gaaagaagct gttccgcata tcccagcagg
     3901 aggagcaatc gtaacaacaa cttctgttca agcattcaat ccaagcgaac atttattaga
     3961 ttacgcagca acaaaagctt caatcgctaa ctttacgatc ggtttagcaa aacagcttgc
     4021 acctaaagga atccgtgtaa atggtgtagc acctggtcca atctggacac cattacaact
     4081 agacgaagga caaccttctg attcgatccc tgaatttggt cagcaaagtt tactagaacg
     4141 tgcaggtcag ccaggagaat tagcaccagt ttatgttttc ttagcatcga ataaagcaag
     4201 ttatgttaca gcccaaattt acggagtaac tggtggagaa gcgatcaact tataagaagt
     4261 tgatcgtttc acccctatga ggaggtgcga aaatgaataa aggtgtaaaa acgatcggag
     4321 tgatcggaac attacttttg ctatctgtgc ttttgtttgc gatactatta agtgcaacct
     4381 atccattgcc ttttcagcta gagaagtttc gattcttttc catcactaat tcatacgtgc
     4441 aacaatatgt attttggctt gccgttgcat ttgctatttt gacaattatc acgttacttg
     4501 ttttcctgtt ttatccaaaa acaatcggaa actttctact gaaacatggt gatggggagt
     4561 tgacgttgga taaaaaagca attgaaggaa tggttcgttc tcacttgcat gaagatgaat
     4621 tcatccattc accaaaagta aatgtcaaag caacgaaaaa caagatcgcc gtcaatgtaa
     4681 aaggggagtt gaaacgaact tcagcactca ttggtaaaac aggaacattg atgaaagaaa
     4741 tcgaaaatga agtacaacac atattgggaa caaaagagcc agtgaaagta tcggtgaaat
     4801 acacaggctt tgaagaacca gctaaacgaa gcaatgaccg caaacgagta gaataggggg
     4861 ggaccaaaaa tgaaagaact attttcgaca tacaagctcc ctatcatctt tggggtactt
     4921 ggattgatat tagccgtttt atggatcacc gtgggctttt tcaagacctt attgatcttg
     4981 atcatggcag cgatcggcgt cgctatcggt ttttatctga aacaaacgaa aatcttagat
     5041 cactatttta aatcttagga ggaaacatcc atgaataaca ataacaaaaa taataacaat
     5101 aaaatgaaca accaaaaacc agtgtctgca catccagacc caacgacacc aaaccaatca
     5161 aatggtgtga acgatgaaaa gaacaaaaca gcaccagtat ctgcacatcc agatccagta
     5221 gtccctaacg ttcaacaagg agtagaagag aagaaacatg atgcagaaaa gcatgcacct
     5281 catcatccag gaactccaga atctaaagtt ccagaacaac atgatgcaat cgatccttct
     5341 aaacctgtgg aagagcaaaa acatccttct gaccatgcga aagcacaaga aaaacttagt
     5401 tctgaccaca caaacggaca aggaaaactt ggttctgatc atacaaataa ccaaggaaag
     5461 catgattctg aacaggaaga ttcaatgcgc ggtgagctga attatgaaga taaagttgtg
     5521 caaaaaatca tcggaatcgc aattgaacaa gtagacggtt tattatctgc aaacggtggt
     5581 ttcttctcta atgtggcagg aaaacttgtg aatacagata atgtaacggc tgggatcgaa
     5641 acagaagtcg gtaaaaaaca agtagcagtt gatatggatg tcattgtgga atatggcaaa
     5701 gatatcgaaa aaatctttga agaaatgcaa gaagtaatcg gcaaagaagt aaaaaatatg
     5761 acacatttag aagtgatcga agtcaacgct aacgtagtgg acatcaaaac aaaagaagaa
     5821 tttgaaaaag atcaagaaac ggtgcaagac aaagtaactg acgcagcgaa aaaaaccggt
     5881 gaatttgctt cgaatcaaac agataaagta aaatcagcag ctggaagcgg cgcacaaaaa
     5941 gtaaaagaaa atacagaacc acgggttcag taataaggaa gtaaaacatt aggaggaacg
     6001 aaacatggga tttatttggt cacttatcgt cggaggaatt atcggagcaa ttgcaggagc
     6061 aatcacgaat aaaggttctt cgatggggat tattgcaaat gtcgtagcag gtttagttgg
     6121 atcagcactt ggtcaagcat tattaggttc ttggggacca agtattgcag gaatggcaat
     6181 cgtgccatca ttgattggtg ccatcatttt agtactaatc gtatcattct ttgctcgaaa
     6241 actgtaaaaa gacttttaaa aagaggtcgt gaaaaagctg ttcttcatca agtaataaga
     6301 acagccaaac aaaaatagct tctcatattt ttcatttggt tgggcatatt atcgaagatg
     6361 caagcttttg aacactgttt atacggaata agaggctgtg acaagacttt tgtcacagcc
     6421 tcttatttat gcgggcaaat tcccaattag atttttaaaa gtttttcctt aaatgtgtct
     6481 ttattgatac agagaagctt ctttaataaa acaatgcctc cctaaatttt cttgagatag
     6541 gaaaggtgaa catacaaaat aatacgacca ctttgaaaac ccagatgatc cacaaatgga
     6601 tcgtatgata ctgatgaagt aaaaagcaat ggatcagaaa ctcaaagaga ttgaaaaaga
     6661 caaggaagaa ataatatctc ttttttactc ttgtctgttt tagccaataa tagctcgtaa
     6721 agtaatctag acaaacaaat agaagtaaac aaagtttcgt aagagcagca atcgtatcat
     6781 gttgctctgc tagacgattg acgagcagga aaaatgttgg taatggaata tagatcacga
     6841 gtaatgaaag aaaaaagtaa acaataaatg gcatactatt cctcctaatt gtgtgagttc
     6901 gccctcattt taaaataacg atatttgttt gtgaagcttt ttcgtgtcgt aacaaatgtt
     6961 atctaatgta tttttaaata aataatcatt attatcaaaa taatgttaaa aataaaaaag
     7021 aattgtccat cactgt
//
LOCUS       DAXWOO010000143         6963 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.146, whole genome shotgun sequence.
ACCESSION   DAXWOO010000143 DAXWOO010000000
VERSION     DAXWOO010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6963
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.146"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            44..265
                     /gene="relB"
                     /locus_tag="ACGE70_002311"
     CDS             44..265
                     /gene="relB"
                     /locus_tag="ACGE70_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFF8693920.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKQLMKEHEENDQSISHEDMLKELGL"
     gene            267..533
                     /locus_tag="ACGE70_002312"
     CDS             267..533
                     /locus_tag="ACGE70_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFF8693921.1"
                     /translation="MTYQVRYEREAQKALKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            700..1185
                     /locus_tag="ACGE70_002313"
     CDS             700..1185
                     /locus_tag="ACGE70_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693922.1"
                     /translation="MGWYGDYSSANEVIEEKKGYAKNGLEIVGTKVTKTYGIILFKDA
                     EGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFFVGDQEKMVKLQLDRIKEE
                     SNKPKLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQDGSLTRFTRLSAGDVKEV
                     T"
     gene            1394..1576
                     /locus_tag="ACGE70_002314"
     CDS             1394..1576
                     /locus_tag="ACGE70_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFF8693923.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKAIGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            1679..1963
                     /locus_tag="ACGE70_002315"
     CDS             1679..1963
                     /locus_tag="ACGE70_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693924.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            1979..2215
                     /locus_tag="ACGE70_002316"
     CDS             1979..2215
                     /locus_tag="ACGE70_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693925.1"
                     /translation="MEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTSLEC
                     VWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            2237..2491
                     /locus_tag="ACGE70_002317"
     CDS             2237..2491
                     /locus_tag="ACGE70_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693926.1"
                     /translation="MNYTDEEQIALEYVFTNEEGEEIEKVLIISSKDLEKALAYEPYE
                     STVTVIEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            2573..2758
                     /locus_tag="ACGE70_002318"
     CDS             2573..2758
                     /locus_tag="ACGE70_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFF8693927.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            2825..3220
                     /locus_tag="ACGE70_002319"
     CDS             2825..3220
                     /locus_tag="ACGE70_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFF8693928.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            3237..3587
                     /locus_tag="ACGE70_002320"
     CDS             3237..3587
                     /locus_tag="ACGE70_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693929.1"
                     /translation="MKATHLNYFSRTLINNLLKDTTGISVSTKHFANDLIIFEDNNLR
                     IEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKKAKLLFEFQT
                     DYHNELLAQSEILD"
     gene            3609..3983
                     /locus_tag="ACGE70_002321"
     CDS             3609..3983
                     /locus_tag="ACGE70_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693930.1"
                     /translation="MKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLWKYKILFL
                     FWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVISDAGIERN
                     IKRVSFKITLNKPTDKKLSEAF"
     gene            complement(4290..4688)
                     /locus_tag="ACGE70_002322"
     CDS             complement(4290..4688)
                     /locus_tag="ACGE70_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFF8693931.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
     gene            complement(4880..5647)
                     /locus_tag="ACGE70_002323"
     CDS             complement(4880..5647)
                     /locus_tag="ACGE70_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFF8693932.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            complement(5682..6842)
                     /locus_tag="ACGE70_002324"
     CDS             complement(5682..6842)
                     /locus_tag="ACGE70_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFF8693933.1"
                     /translation="MKITEKNVNPKFETTESNLKKFDLTVTLSNQLVDSIVEMDAMPQ
                     KIYLYAIGHLDPKNPPFNNTVKVSKKALFALMSSSSSSSKHSRLKQVIEKLHQQSVIK
                     IQRTKPNGKTEYKVISPLEITEFNDFSDAVSIKFSSSIMPLLTNLENHFTQYTFDQVV
                     NLTSKYAISFFQLFTKDFNLYEANRDNLTWSTKSLDRLKNPVFSIADLRKKFSIEKKY
                     KAFRDFERRVIKEPIAQLNKYTDFKIDYELIKKGHTYTNIKFLLTRTMSVPVPYKQEQ
                     RDPAYLKGAEDKTEKMNQSYLKAMNSKYTKLLIKNFLISSADLCDLKLMSRLQNKVYP
                     LYDRLSKDRGFESVVNHLSYVATHKEDYTIKNTVKYLEKSITSYLPYVNLYD"
BASE COUNT         2375 a         1162 c         1146 g         2280 t
ORIGIN      
        1 atacaatcgt agtacaatgt aattgaaaag gagttgataa actatgccag taataagtat
       61 tcgtgtatct aaagaagaaa aagattttct tgaaaaagtt gctgaattca acggagataa
      121 catttctgaa tttatgcgta gtaattccct gcaaacagca gaaacgttag tagactttgc
      181 cacctacaag caactaatga aagagcatga ggaaaacgat caaagtattt cccacgaaga
      241 tatgctaaaa gagttaggct tataagatga cttaccaagt aagatatgaa cgtgaagctc
      301 aaaaagcgct aaaaaaaatg gataagtttc aggcaaaggt tatcctaaac tggatcgaga
      361 aaaatcttgt aggcactgat gatccaagaa agcatggtaa aggtctaaca gctaataaat
      421 caggctactg gcgatatagg gtaggtgcat accgtataat agctgatatt agcgagcatg
      481 aagtgacaat tcttatattg tctattggtc acagaagtga agtttataag taacagacaa
      541 gctgataagg cttgtctgtt tttttgttaa cccaacaaaa tcactgttta cgatcccact
      601 ttacctaaaa aagaacaata tcctcttgat attatacaca ccagtgtgta taatataagt
      661 gtaaacaagc taattaactt tcaaaaggag cgatttttta tgggatggta tggtgattat
      721 tcatcagcaa atgaagtgat cgaagaaaag aaagggtacg ccaaaaatgg tttggaaatc
      781 gtaggtacca aagtaacgaa gacctatgga attatccttt tcaaagatgc agaaggaacc
      841 tacgatattg acagcttcat ctttcgagat gaaatgtaca aaccaatcta cttcttaaat
      901 gacagtcctg cttactttaa gaaaattccg aaaaaatggt tgaaattctt tgtcggtgat
      961 caagaaaaaa tggttaagct acaactagat agaattaaag aagaaagcaa taaaccaaaa
     1021 cttgatgact tattagttgc aggtaaaact tactcaatct gggatggatc gtatgtcgct
     1081 gtctatcagt ataaactcaa acgtagcttt atttttcgac tacaagatgg atcactcact
     1141 cggttcactc gactatcagc aggagatgtt aaggaagtaa cctaaaactt aaatacataa
     1201 acataaacgt gtatacgttt atgtttatgt atatgtatat gaatataaac gtattaggta
     1261 cgaacacttt taaattcttt tctgggcttt gtttttaggg tagggctaaa aaaaacatca
     1321 ccatctgatc caaaacagct cagaaacgca tttaaacgta tctgatacca atattacaga
     1381 aggagtgtat cgtatgatta acctatttct tttagtgaca gctagtacat tattctttac
     1441 tggaatatca ctgataattt cctatttgat ctataagaaa gccgaaaatc acacaagcaa
     1501 agcaatcgga ctagccgttt catccttttt aactttgctg actttatttg ttactttatc
     1561 gttttctttt gtttagaaca gctgatccat tagcaccttt ttctctttac attatacaca
     1621 ctgttgtgta taatgtattt gtaaacaagc taattaactt ttaggaagga gtgacaagat
     1681 gctaacagct actttacagc aacctactca cgttgctatt gcagaaagtt atacaattaa
     1741 tgcagggatc gttaaacagc tgatcaagga tcgagaattc aaaaagttta acagtcggca
     1801 agcgtataac agacagctaa agaaagctat tctaaagcac aaagattcct tcatcgggaa
     1861 cttggaaagt gtagatgtta aaactgtttc aacggcattt tcagttgttg aagtgactat
     1921 tgacggtcgc ttgatttctc tatttgcttt tggaattaat tgatagggag tgtgaacttt
     1981 ggaaggtaag acttttgcag aaatttggac tgaatttatt gaggtaccaa ctcgcattga
     2041 tccgataact tctgaaaagg tattggatgc agacttctac aatctcaaag caggtactag
     2101 ccttgaatgc gtttggatgt atttagaaga gcatttcaaa ggcgtaacgc tcggtcacgt
     2161 cttataccac aatggtgccg agtgggatgc aattggaaat atccattttt cttaatataa
     2221 gaaaggagaa cacaatatga attataccga tgaagaacaa atcgctttgg aatacgtttt
     2281 cacaaatgaa gaaggggagg aaattgaaaa agtcttgatc atttcatcaa aagacttgga
     2341 gaaggctttg gcttatgaac catacgaatc aaccgtaact gtcattgaat tcttagatac
     2401 ctatacttct aatgatactt gggaaattat gaatatttgc aacgctaata acattcaatt
     2461 tgctgaaaaa accaataaat cattttatta gttttgcaat aaaacatcct ctttttctat
     2521 tgattccata cacaacaatg tgtataatag attttgtaaa gggagggtag ctatgcctaa
     2581 gacagcaaga gaaattgtaa aaattctcaa atctaacggc ttcaaaaaga ctggacaaac
     2641 tggcagtcat gcaaaatttt ataattccaa gactaaaaga acggtcgttg ttccgatcca
     2701 caatggtgat gtaccactag gaacagaagc taatatctgg aagcaagcag ggctttaaca
     2761 gccttgcctt cctatttggt attaggcata ctatcctctc ttacactaga aaaggagcga
     2821 ggaaatgaag acaaaaaata ttttttatcc ggcaatcttc gagaaagaag gagaagctta
     2881 taacatcgtt ttccctgact taccggaaat atctaccttt ggaaatagtc ttgaagaagc
     2941 atatttaaac gctaaggatg ctttaggatt agcgctatat agtgtaccag atgcagaatt
     3001 tcctaaacca tcagcagtgg aagaattaac tttgaaagcc aaccaagtgg tagtaatcat
     3061 ccaactaaac attaaacttt tccgaagaag cttaaataca aaaacaatta gaaaaaatgt
     3121 tagtgttccg gaatggttag ttcttttagg gaaggagaaa aacataaact tctctcaact
     3181 acttcaaaaa gcactagaag aagagttgct tgaaaagtaa aaaggagacc gaacctatga
     3241 aagctacaca cttaaactat ttctctcgaa cgttaatcaa taatctatta aaagatacaa
     3301 ctggcattag cgttagtacc aaacactttg ccaatgatct aattattttt gaggataata
     3361 acttgcgaat tgagatgttt attgaacagg caacccctaa ctcaatacct gaattctgct
     3421 atacgcacac tgaaaaaatg aaacatgcaa aatcagttaa agatgttgaa gcattaagtg
     3481 aatcactaac gaacgcttta aacctcgtta agaaggcaaa attgttattt gaatttcaaa
     3541 ccgactacca caatgagttg cttgctcaat ctgaaatttt agattaattg aaacaggaga
     3601 agagaaagat gaaacaaaca caaacagaaa ccgttaacga tacgcttgct gatcaattag
     3661 caggttatta taaattattt gagatgggta aagcacagcg cttgatccac tatctttgga
     3721 aatacaaaat tttgtttctt ttttgggaat caacactaac attctcagtt cttttttata
     3781 agtcagattt ttataatcaa cacttagttt tagggagttt tttgcttatc tacaccattt
     3841 tgatgcttaa tttatatacc tacatcacac ttgaagaaca cttagtaatt agcgatgcag
     3901 ggatcgagag aaacatcaaa agggtatcct tcaagatcac gttaaacaag cctaccgata
     3961 aaaagctctc agaagcgttc tgagagcttt tgacattttc ttgtataatt ttatcctaac
     4021 acattacaaa gcacctaatt cgagcttata tcaagcaaat ttgctattta ttactcaata
     4081 catatctaaa aattacttga tggaaaatta gatacatggt aagcatgaaa agaaaatcaa
     4141 tttgagcaga tgcagtacaa tacatagcaa tttaaaagct ctcagaaggc ttctgagagc
     4201 ttttaatata ttctttgata atttgtcttt cttaaatcca tacgtcctaa aacaagctgt
     4261 ttttgcttaa tatgatcatc tgagagacat caaatcaact tttctctcaa caaatcattc
     4321 aaagctttcg acagattaat attctcttca gccgctaaaa catttaaata gtgttccaca
     4381 gtcactgatt ttcgatcata attttgcttg tttcgagctt tagcaacttt cgtattagct
     4441 tttacgacaa ataaattaac attctcagat cgcttgattc ttgcatttaa ctcttgaaca
     4501 ggcgtaggcg atggaatttc tttttcttgc aactctaaaa gcaccaagta tccttccaat
     4561 acttcttgcg ccatctcaaa agcatcttct aaagtttcac catgtgtata taagtctgga
     4621 aaatctggga atttaactgc atattcattt cctttccatt cgaatactgc acaaaaaaag
     4681 tatttcatcg cattgcacct ctctaattaa aaaataattt ttgctttttt gtttcaaaaa
     4741 aaagaatcgc ctaccaaaaa aattgatagg cgatcacaag ctaattaacc tctaaaggag
     4801 attaatacct tgctcccaag gcactatata tcttagcata cgtctgaagc atcttcaaac
     4861 gagtaagata tattttgcat taacctaatt ttttttctct ataagttttt tcatcaaact
     4921 cactagaaat attaaactgg acagcaaaat tgtaatttct gccttttcta actttagcaa
     4981 aatcaatatt cagcattgat ttttcattaa tttcctcaac tgattccctt aaaacacgtc
     5041 tatcaaaatt cgatattgtt ttatactcat tttctgtttt cgtaatctca cgaattttaa
     5101 ctatttcaat caggggattc ttatatattt cagctttctc aaaatctcct ttatttttgg
     5161 cgataagata ttttttataa tacatcgcca aatatttgta taacacgatg ctatattttt
     5221 tttgaaaaga tgctaagtct tcaaaaacaa agaacggata cttctttttg attgagacaa
     5281 ttaagggaat tacatcttct gaaaaactga aagatacact atgctcttct aaatcccaat
     5341 gaatccgatc aatagcagat acagtagtat acgtcttata ttttttgtct agattcgaaa
     5401 tttcataaac tgcttgtctt tgcaaagtgt taactatggt ttttagttcc gtttcaagat
     5461 tttgatcaac atccatgtaa ttaataatcg ctttaacagg taaatctact acgcctgttt
     5521 tctttagctt tgtgggtgag atataactga acagcaattg ataaagttca tacgacttta
     5581 aatccatttt catgtcaatt gttgctaaga actcctcatt gtactttgtc agttcatctt
     5641 ttttcaaaag tttttccttc cttcacgcat tttttcagtt gttaatcata aaggttcaca
     5701 taaggcaaat aactcgtaat tgatttttcg agatatttaa cagtattttt tattgtgtaa
     5761 tcctccttat gagtagcaac gtaagatagg tgattgacaa ctgattcaaa tcctctatct
     5821 ttggataaac gatcataaag cggataaact ttattctgaa gtcggctcat aagtttaaga
     5881 tcgcataagt cggcagaact gatcaaaaag tttttgatta gtaatttagt gtatttgcta
     5941 ttcatagctt tcaagtacga ttgattcatt ttctctgttt tatcttctgc gccttttaaa
     6001 taagcagggt ctctttgttc ttgcttataa ggtaccggaa ctgacatggt acgtgtaagc
     6061 aagaacttta tgtttgtata agtatgaccc ttcttgataa gctcatagtc aattttaaaa
     6121 tcagtgtatt tattcagttg ggcaatcggt tctttaatta cacgtctttc aaagtctcga
     6181 aaagctttat attttttctc aatactaaat ttctttcgta aatctgcaat ggagaaaaca
     6241 ggatttttta atctatcaag tgattttgta ctccatgtta agttatctct atttgcttca
     6301 tacagattaa aatctttagt gaataattga aaaaaactga tcgcatactt gcttgtcaag
     6361 ttaactactt gatcaaatgt atattgcgta aaatgatttt ctaagttcgt taataaaggc
     6421 ataattgaac tactaaattt aatagatact gcatcagaaa aatcattgaa ttcagtaatt
     6481 tctagcggtg agatgacttt gtattcagtc ttaccgttag gtttagttct ttgaatttta
     6541 atgacagatt gctgatgcaa tttttcaata acttgtttta atcttgaatg ttttgaagaa
     6601 gatgaactag aagacatcaa cgcaaataaa gcctttttag atactttgac ggtattatta
     6661 aaaggcggat ttttagggtc aagatgtcct atagcgtaaa gataaatttt ttgtggcata
     6721 gcatccattt caacaatgct atctacaagt tgattcgata aagtaacagt taaatcaaac
     6781 ttttttagat tgctttcggt agtttcaaat ttcgggttta cattcttttc tgttattttc
     6841 atacgattgc ctcttctttc taagcttggt acgagcttaa aaacttggaa ctggtacgat
     6901 tccattgaat aagtatagac ctgttttcga tacgttacaa taagaaatgt atcgtaaatt
     6961 tat
//
LOCUS       DAXWOO010000144         6951 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.147, whole genome shotgun sequence.
ACCESSION   DAXWOO010000144 DAXWOO010000000
VERSION     DAXWOO010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6951
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.147"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..1153
                     /locus_tag="ACGE70_002325"
     CDS             <1..1153
                     /locus_tag="ACGE70_002325"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFF8693934.1"
                     /translation="KIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAVN
                     RANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFAR
                     SFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAMM
                     ITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKEL
                     SGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNLY
                     TGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQEM
                     LDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            complement(1175..1927)
                     /locus_tag="ACGE70_002326"
     CDS             complement(1175..1927)
                     /locus_tag="ACGE70_002326"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFF8693935.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(2291..4957)
                     /locus_tag="ACGE70_002327"
     CDS             complement(2291..4957)
                     /locus_tag="ACGE70_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFF8693936.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5298..6749
                     /gene="cls"
                     /locus_tag="ACGE70_002328"
     CDS             5298..6749
                     /gene="cls"
                     /locus_tag="ACGE70_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFF8693937.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
BASE COUNT         2042 a         1447 c         1267 g         2195 t
ORIGIN      
        1 gaaaataggg attgatcgtc tttccttttt tattcctaat ttatatttag atatgacaga
       61 gttggcagaa agccgtgggg atgatcctgc aaaataccat atagggattg gtcaagacca
      121 aatggcagtc aatcgtgcaa atgaagacat cattacacta ggagcaaatg ctgccagcaa
      181 aatcgtaaca gaaaaagacc gtgagctaat cgacatggtc atcgtcggaa cagaatccgg
      241 gattgatcat tcaaaagcga gtgcggtaat tatccaccat ttactgaaaa tccaatcttt
      301 tgctcgttct tttgaagtaa aagaggcttg ctacggtggc accgcagctt tgcacatggc
      361 gaaagaatat gtcaaaaatc atccagaacg aaaagtacta gtcatagcaa gtgatattgc
      421 tcgttacggc ttggcaagcg gtggtgaagt gacgcaaggt gtcggtgctg ttgcgatgat
      481 gatcactcaa aacccgcgta ttttatcgat tgaagacgac agcgtatttc tgacagaaga
      541 tatctatgat ttctggcgtc cagattatag cgaatttcct gttgttgatg gtcctttatc
      601 taattctacg tatatcgaat cattccaaaa agtttggaat cgacataaag aattgtcggg
      661 tcgaggactc gaagattatc aggcgattgc tttccatatt ccgtatacta agatgggaaa
      721 aaaggcattg caaagcgtat tagaccaaac agacgaagaa aatcaggaac gtcttatggc
      781 tcgctatgaa gaaagcatcc gttacagccg acgaatcggt aatctttaca ctggttcatt
      841 atacctgggg ctaacttctc tactggaaaa ttcgaaatca ctacagccag gagatcgcat
      901 cggtctattc agctacggtt ctggtgcagt aagtgaattt tttacaggtt atctggaaga
      961 aaactatcaa gaatatctct tcgcacaatc tcatcaagag atgctagatt ctcgaacacg
     1021 gatcactgtt gacgaatatg aaacgatttt cagtgagacg cttcctgaac acggagaatg
     1081 tgcagaatat acttcagatg tacctttttc gattacaaag atcgaaaatg atattcgtta
     1141 ctacaaaata taataaaatc ataaaaagaa cccttcacga gtcgtgaaag gttctttttt
     1201 tatctgcgga ccaaaggagc agtctatagg tcagcttagt cgtactaagg atttctcgtt
     1261 tttcactgaa tagtttgcga atgtacctgg tcacaacagg atcttttgcg tacgcgtcat
     1321 agtatagctt catcaattct tcagtaattg attcctgtgt aaaataagta aaatcttttt
     1381 tcttcacttt gaatccttca tcaattaagt atgtttcaaa ttgatccatt acaactggcg
     1441 atgtattagg atcttcttct atcattccta gttgcttttc gataggataa aaaacatcat
     1501 cgattgattc cactactctg attatcacgc accatttgct tgactttttc atcaattttt
     1561 ctaattgaat aagatgtacc aatgctggat ttgctgctgt aaaaaccatt tcatatgtct
     1621 catttgattg gataaattct tctaacgact gtttgataaa ggaaacattt ggcaactcat
     1681 ttttttctgc ttcttttttt gcattttcta gcattcctga agaaaaatcg atcaaatcat
     1741 agcgattaat ttcttttgct aaggaaagaa atctgccaga tcctccagca acgtctgctg
     1801 ctgtttcagc aggcaggact ttctccgttt tcagataatt ttttacatct tcagcaatag
     1861 ttacttgcga ttcttgctgg atttcataat attcttcagc aaattcatcc cattcttttt
     1921 cattcatttg atttcctcta attttttgtt atatgttttc acttttcgga ataaatcatt
     1981 ctcctctttt atctattcta tctcttttga tgaataataa aatgattggc tttccgtcta
     2041 tcataaataa aaaaagggac tgtgaccaaa gtcgtgtcaa agtccctctt tccgaataaa
     2101 cagtgtccaa tcagatgacc ctcagtatta gctcaaaatg ctgattttac aacttcttcc
     2161 ttaaacggtg ttctcctgcc aactcttcgg tcacaagtca caatattcaa caaacatgag
     2221 aagctgtttg ttgaaattgc accttgtgac tcagaattaa acggcaggct cacaacctcg
     2281 ttgattcgtt ttatttattt ccttgtactc gtacaatgat caatttagtg atttccatca
     2341 aaacaattgt cacaagtgcg attcccatac atgtaaagac ttctgtcaat ccaaatgctg
     2401 atgggataga gaagacttct ttcagtcctg ggatcattgt taagcagtaa agcgcgctac
     2461 atacaactac agctagtaag acacttctgt tagagaagaa ccctgcttgg atagctgttt
     2521 gtgtgtttga acgtgctggg aatgtttgca atgtccgaca ccaaatcaat gttgaaaatg
     2581 acatggctac accgaattca ggtgaatgct gcatgccaag ccattgagca aagataacag
     2641 caatcccaat cagcacacca cgatacgtta cagaaatcaa tgttttgtcc gtaaagattc
     2701 ctgaatttgg atctcttggc gggcggttca tcgtagcagg ttcagctttt tctgtaccta
     2761 atgcaattgc tggcagtgaa tcattgacta aattgatgaa caacagttgt aatgctgtaa
     2821 atggattgga ccagtcagca actaaggcaa agatgatcgc aatgatcgca cctaaatttc
     2881 ctgcaaatag ataagaaata gctttcttga tattatcaaa cacattacgt cctacttcta
     2941 ctgcgctgac aatagaagca aagttatcat ccgtcaatac catcgctgct gcatcttttg
     3001 ccacatctgt acccgttccc atagcaattc cgatgtctgc ttgtttcaat gctggtgcgt
     3061 catttacacc atcacctgtc atagctgaga tatgaccttt attttgccaa gcacgaacga
     3121 tacgaatttt attctcaggt gaaacacgtg cataaacagt gactttttct aaaacttcgt
     3181 ttagttgatc ttctgtcaat tcatctagtt ccgtcccggt caaagccagg tttccttctt
     3241 caaaaatccc gatttgtttt gcgatagcaa cagctgttgt tttgtggtca ccagtaatca
     3301 tgattgtttt gattcctgca cttcttgctt ctgttaccgc ctgagtcact tcagcacgtg
     3361 gaggatcgat catagcaagc aaaccaacaa ggatcaaatc gtcttcatct tcaaatgata
     3421 aatcaaaatt atcaagtgga cggtaagcaa aagctagtac acgtaatgcg cgtgttgaaa
     3481 aagcttcatt ttgctcttgg aattttttca tcaattcatc cgtcatcgga acgacttcac
     3541 catttagcaa tactttttta ctgcgttgga agacgatatc tggtccgcct ttaacaaaca
     3601 agtaattttt gttatcaatt gtgtgtccag tagacattaa ttttcgatct gagtcaaaag
     3661 gaagttctgc ttgtcttggg tagttgtttc ggatttcctg ataaggctgg tttaattttt
     3721 cactaaagtc gataaacgct acttccgtag ggtcgcccaa tttagtaccg tcttcactga
     3781 tcgatgcatc attcgctaaa acactgattt cgatcaagcg acgttcgtca gctgtccatt
     3841 ttgaagggtc atttgtgaaa tccccagtat cgccattacc taaaaagtag tcaacaactg
     3901 tcattttgtt ttgtgtcaat gtaccagtct tatctgtaca gatgatactt gtcgaaccaa
     3961 gtgtttcaac tgctggaagc ttccgaataa tggcatgacg ttttgccatt ttattcgttc
     4021 ccaaagataa cacgattgtt acgatagact gtaatgcttc tggaatagca gctacggcta
     4081 cggctacggc aaacatcact gcgctgacga tatctgcagt catatctcct gtaccttctc
     4141 caagataaat acgaaacagt tggataccta aaatcaataa agacaatgcc aaaatagcca
     4201 aacttagttt tttactgaat ttatccagac ctcgttgtaa cggagtgact tgctcagttg
     4261 ttgattccaa aaggccagca acttgtccga tctccgtatt attcccagtt gaagtcacta
     4321 agaatttacc gcgtccgtaa gttacgatcg ttccactgaa taccatattt gtacgatctc
     4381 cgattggtac cgtacttgtg atatccgtta cttctttttc tgcagaagtc gactctcctg
     4441 tcagcatacc ttcatctact tttaatgatc ccgcatctag taaacgtcca tcagcaggta
     4501 cataatcgcc tgcgtctaac aacacgatat ctcctaccac aagttcgttt gccggaatcg
     4561 ttttttcctt accatcacga atcactcgag catcgggcgc tgaaaggttt ttcaacgctt
     4621 ctaaagaacc ttctgctttt tttgtttgga caacactgat tatagaattc aacatcaata
     4681 ctgcaaaaat aactaagaat tcgatgtagt cacccatgac catctgaatc agtgcgacaa
     4741 tcagcaatac gataaccatt gcatctttga aagtttctaa gaacaatttc caagttggct
     4801 tcttcggctt ttcttttagc tggttcaagc catctctttc taatttttcc ttcgcttgcg
     4861 tggtgctcaa tccatcggtt gagctctgcg tttgctgcat caacgagtcg atcgtttctt
     4921 tatagaccgc ttgttgtgct tgttccttgt tttccaaatt tcatccccca ttttttgatc
     4981 ttggccctag tcctgtctga aaaaaaagag acctaagaca aatacgcatt tgtcttaggt
     5041 ctcaccattt catacaatac cagaaaacca tttggttaac ttactgttga tattatcgca
     5101 acaaacgttg ccggttactc ccctaaggag atattcaact gtactaatat cctaccttat
     5161 taacctacaa aaagcaacta ctttagcccg atttacctac attttcatga aaggagtgaa
     5221 aagtatattc accttggtag gcttctttct tctatcatgc tatactaatg ttattgtatg
     5281 taaggaggaa catttttgtg gtatctagta ttataaccgc cctttatcta ttaaatgcac
     5341 ttattgcttt ggtggctatt ttgatcaaac cccgagatgt agcagccatt tgggcatggc
     5401 tcttagtatt gtttgccctt cctggggtgg gctttgtatt atatttattt ttcggacgtg
     5461 gattaacgga taagaaaaaa ttctatctcc gacaaagtga cttgaaagaa ttagaaaact
     5521 ttcagtcttt tagaggagat accattgaac attacgatcc tgacatgggc gataaagaca
     5581 aacagcaatt tgttgacttc ttctcttcat taaatcgtat gccgctgaca agaatgaatt
     5641 ctgtcactct tctcacagac ggacaagaga aattggattc actgcttcaa gatctaaaaa
     5701 aagccaaaca ttcgatccat atcgaatatt acgcatttgt gacagataat atcggccagc
     5761 aagtcttaca tgttttagaa gaaaaagccg cagaaggcgt ggaagttcga atattatatg
     5821 atgcatttgg ctctcatggc acaaaagcaa aagatttcaa tcgtctaatc aaaaatggtg
     5881 gacatgtcca tacatttgtt acctcacaaa gggcattact tcgtttccga ttgaattacc
     5941 atgatcaccg aaaaatcgtt gtgatcgatg gaaagattag ttataccggt ggtttcaata
     6001 ttgccaatca atatgtaaat acaacaaaaa aattcggcta ttggcgcgat acgcatatac
     6061 ggattttcgg tgccgcttct tctttgctcc agcttcgctt cttaacagac tggaacgtct
     6121 cggtacctga agaaaaaaag gtcggctatc atttgaatta tttctttaaa aaagcagatc
     6181 gagatgaatc taagcttgct gatacatcca tccagcttgt ttcaagcgga ccgaataacg
     6241 aaagggaaca aatcaagctt tcatttatca aattgattac ttctgctaaa aaacgtgttt
     6301 ggatacagac accttacctt gttcctgatg aaagtgtcat tgctgcttta aaaatcgcaa
     6361 ctgcctctgg tgtagatgtg aaaatcatga ttcccaacaa accggaccat ccttttattt
     6421 atcgagcaac acaatattat gctcggcagc tgatcaagga aaatgtacaa atccttgtct
     6481 atgagaacgg cttcctccat gcaaaaacat tgataatgga tgatgaaatc tgcatggtag
     6541 gttcagcaaa tcaagatatt cgtagctacc gattgaattt tgaaacaagt gctgtcattt
     6601 acgatcctga gtttttagaa gaacttgcta ctcagttcaa agaagatgag acacattgtt
     6661 catccatgac aactgaaaca gtcaaggaaa tgtctaactg gctattattc aagcaacaaa
     6721 tttctcgatt attttctcca atcctataat ttttttattc aagtagagaa aaatatacta
     6781 agattaaagg gactgtgaca aaaatcttgt cacagtccct tcttccgaac aaacggtgtt
     6841 ctcctgccaa ctcctcggtc acaagtcaca atattcaaca aacatgagaa gctgtttttt
     6901 gaacttgttc cttgtgactc ggagctggac ggcaggctca cgacctcttt c
//
LOCUS       DAXWOO010000145         6922 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.148, whole genome shotgun sequence.
ACCESSION   DAXWOO010000145 DAXWOO010000000
VERSION     DAXWOO010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.148"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            197..1069
                     /locus_tag="ACGE70_002329"
     CDS             197..1069
                     /locus_tag="ACGE70_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFF8693938.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            1066..3024
                     /gene="nagE"
                     /locus_tag="ACGE70_002330"
     CDS             1066..3024
                     /gene="nagE"
                     /locus_tag="ACGE70_002330"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFF8693939.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(3275..4126)
                     /locus_tag="ACGE70_002331"
     CDS             complement(3275..4126)
                     /locus_tag="ACGE70_002331"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFF8693940.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            4249..4716
                     /locus_tag="ACGE70_002332"
     CDS             4249..4716
                     /locus_tag="ACGE70_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFF8693941.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(4979..5461)
                     /locus_tag="ACGE70_002333"
     CDS             complement(4979..5461)
                     /locus_tag="ACGE70_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFF8693942.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(5561..6424)
                     /locus_tag="ACGE70_002334"
     CDS             complement(5561..6424)
                     /locus_tag="ACGE70_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFF8693943.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         2079 a         1426 c         1248 g         2169 t
ORIGIN      
        1 aattgacaaa acataactgt ctatataaaa ttgattacgt taacaataat tcgtttcatg
       61 tgactagatc atactaggca tgaaaaatct ctgaggaaca tggtttccct aggaggtttt
      121 tcgtgtctat ttttgtttgg ccgcaatcaa ctggtctagc aaacgtgaaa accaaatctt
      181 caaaaagggt gcgcgcatga aaatcaaaaa gatctttaat caaaatgcag tattggtaga
      241 tgacggtgga caggaaaaag tagctattgg aaaagggatt ggatttgaca gaaaaagaaa
      301 tgatttgatt tttgatcgtg atgtcgaacg gatatttgtc atggaaccag aaggtcaaat
      361 caagttgcaa aatttactta atcagattga tgaacgcttt ttatttgcag cagaaacgat
      421 cattgatcat gcggaaactg ttttgatgga aaagttgaat gaacatgtat tgatcgcttt
      481 gaccgaccac attgcttttt ctgcggagaa cattaataac ggtatcgtca tccgcaacaa
      541 acttttaaga gaaatcgaag tgctttacag tgatgaattc agtattgctc aatgggcagt
      601 agagtatctg acaaaagagc ttgggattcc atatacttat gacgaagcag gatatatcgc
      661 aatccatatt cacagcggac gaagcggtcg gaacaataac catcgcagta tccgtgaagt
      721 gacgattgta tctgacatca tccatttagt ggaatcagaa ttggaaacag atatccattc
      781 agaaccattt tcactgaatt attcaagact ggtgaatcat ttacgactgc ttctacaacg
      841 aacccatgcg caacaatatg ctgttttaga cacggaaatc gtagaaatgg tcaaacgaaa
      901 atacccagaa agctataaga tttctaaaaa aatccgtgtg ttgctgacca aagaatacca
      961 gctcgcgatc acgaaagaag aactgggtta cttggccatc catatcgagc gtttaagaag
     1021 cgcaatcgta caaacaaatg ttaacaatca tgaggaggaa aaaaattgaa agcatatcta
     1081 caacgaatgg gacgttccct tcaacttcca gttgcggtct tacctgctgc tgctttacta
     1141 gtagggatcg gaaactggtg ggcatcttat agtaacgaca ttgtcgctca ttttttacaa
     1201 gctggcggta acgcagtttt aggtcaatta cctttgctgt ttgccgttgg gttagcacta
     1261 gggatgtcag tggacaaatc aggtgctgct gccttggcag gtttggtcgc atttgaagtt
     1321 ccagtaaatg ttttaaaaac tgattcagta gctactttat tgaatatcaa accagaatca
     1381 gttgatccat cttttgctca gatcagtaac gtatttattg gtattattgc cggattgatt
     1441 gcggcagctc tttataatcg tttccacgaa gcaaaattac cgatggcttt atctttcttt
     1501 agcggcaagc gtctagtgcc aatcttggct gcctttgtca tgttgctgat cagtgcagtg
     1561 ctactgctcg tatggcctcc ggtttactct gctttagttt cttttgctaa attcattttg
     1621 agtttaggct ggggtggtgc aggtctttat ggcttcttca accgtttact gatccctaca
     1681 ggattgcatc aagcaatgaa ctccgtgttc tggtttgacg tagcaggaat caatgatatt
     1741 ggtaaatttt tggcttctga aggaacaaaa ggcgtaacag gaatgtatca agctggattt
     1801 ttcccaatca tgatgttcgg gcttccagca ggagcatatg ctatttacag aaatgcacgt
     1861 ccagaaaaca aagcaaaaac agcttccttg atgctagcag cagcttttgc ttcattcttt
     1921 actggtgtga cagaaccact tgaattttca tttatgttcg ttgcttggcc attatatgtc
     1981 ttgcatgcaa ttttcactgg gatttcttta gcagtgagtg cattcttcca ttggacagca
     2041 ggatttgcct ttagtgccgg atttgtcgat tacttcctat ctttgaaaaa tccgattgcg
     2101 aatcagccat tgatgttgat cgtccaagga ttagtaacag cagtgattta ctactttggc
     2161 tttaactttg ctatcaaaaa attccatctg atgactcctg gccgcgagga agcagatctt
     2221 tcagatgacg atactgcaac gactaattca gacaataaat atgcggcaca agcaagcaaa
     2281 atctacgctg cattaggcgg agccgacaat gtaacttcga tcgataactg tactacacgc
     2341 cttcgtttgc aagtcaaaga tacaggcctg atcgatcaaa ataaaatcaa agccactggc
     2401 gtacctggta tgaagatcat cgacggaaaa aatgcgcaag tcatcgtagg tacagaagta
     2461 caattcgttg cagatgagat ggcaaaacta catggtggtg ctgcagcacg acctgcacaa
     2521 acaaacaccg tggttaaaac agaaacattt gccggtgaaa cgatcgaaca agatatttac
     2581 gctatcgcaa atgggaaact gattccaatc actgaagttt cagatgatgt attcgcggaa
     2641 aaaatgatgg gtgacggcta tgctgtatta ccagaaaatg gagaaatctt ctctccaatc
     2701 gctggtacga tcacaaacat tttcccaacg aaacatgctg tcggaatcca aacagatgct
     2761 ggtatcgaag tcttgcttca tatgggcatc aacactgttg acttaaaagg cgaaccattc
     2821 actttatatg tagaagaagg tcaaaaagtc gcaagaggtc aattgattgc tttagttgat
     2881 cttgccgcta ttcaatctgc aggcaaaaac acggatatgg tcgttgtatt cacaaatggc
     2941 gacaaagtcc aaagcttaga aatcgaaccc gcacgtgatg taaaagcaaa tgataaaatc
     3001 ggctctgttt ccaacaaagc ctgagccatc gtcttcccca gactttttta tcgataaaca
     3061 aacaggagct gagacaaaaa tcttgtctca gctccttttt ccgaataaaa acggcgttct
     3121 cctgccaact ccgcggtaat aagtccagct aaacgaaaca tatgaagagc tattttttat
     3181 ttgactcttc ttattacttg atgcagaaca gctttttcac aaaatttttc ttcatactca
     3241 aaactaatcg gctttttcac aacctcttta tacattattc ctgatccaat atttctttta
     3301 atgctgcttc aagagaagtt aatggttttc ccaatacttt ttctaaatca tcagaggaaa
     3361 tagctaactg atccttttta atatcatgtt ggatcgctaa aaacatgtca gctgcttctt
     3421 gagggaatcc ggcttctttt agattttcga caaacgctgc atcacttgct tcaataattt
     3481 ctaactcttt acctgtcgca cgctctaatg ccttggctaa ttcttcatag tcagttagat
     3541 tgccggataa ttctaagatt tctggagagt cactttctac tagtactttt gctgcagctt
     3601 ctgcatattc acgtttcaat gcccaacctg tttttccatt tcctgcagca taaacgaatt
     3661 gaccagtttt taaagcctgc ccaatgatag gtaattcatt ctctacatac caattattcc
     3721 gtaaaaatgt atgggaaata cctgaatcct taatcatttt ttcagtgaat tggtgatctg
     3781 gcgctaatac actgatcgag tgatcggcat ctgcaaaact tgtataagcg atataagata
     3841 ccccagctgc ttgtgctgcc ttcactacat tcttatgttc ggcttgacga tttcctggaa
     3901 cgccagagac aaaaagcaat cgatctattc ctttcaacgc ttctttcatt gagtctaaat
     3961 catcataatc accaatacgt atattaaatc cagcttcttt cagtgcttct cctttttctt
     4021 cactacgtac tagtacatag atttcatttt gtggaaccat cttttttaag aattccagcg
     4081 cataaccacc aaaacctcca gttgctcctg ttactaaata atgcatattt gccactccct
     4141 attcttttta tttgttaaga ttcacagctg tacatttata aattacacct ttatcataaa
     4201 gagtgtggta agataagtca agcaaaaaaa atgaaaggat gacaactgat gaagtattct
     4261 ctgcaattta gtgatgcaat ccatgtactt gcctatattg aaatgtttca agatacccga
     4321 tcactttcta gtgagatgat tgccagtagt atcgaaacaa atgcagcaaa tgtacgtaaa
     4381 atcatgagtc agctcaaaaa atccggtttg atctttacaa aaaccggaaa accgcagcct
     4441 tcccttgcca aaagtccaga tcagatttct ttactagatg tctatcgcag catcgaaggt
     4501 aatacacagc ttatccaagt cgatccaaaa acaaatccaa attgcgtcgt tggtgcgaat
     4561 atccaagaag ctttagaaga aagctacgct cggctgcaga aaaaggcaga agaagaaatg
     4621 gctaagatca cacttgatag tctgatacat aaaatagccg ttttggaaaa agaaaaaaga
     4681 ccagaaaaca caggcctcgt agagagattt ttataattct ctctactgtg cctgcatttt
     4741 tctgatcgga atcgtctaca taaattcttt tattctccgg gggacaaaag ccttgtcatg
     4801 cccctttttc ctctctaatg ctcaggtcaa agcgctgaag tcatgacgtc ttgataaacg
     4861 gtgttcaagt agctgaccct cggcattagt tctgaaaaat gcaaaatatg gaaagctatt
     4921 tcccattttt cctctctaat gctcaggtca aagcgctact ttcacgacct ctatattatc
     4981 atggtttatt gactaccggt atccagattt ctcccatata tttcccgttg gtaactccca
     5041 tttcgactac agcatttgga cccccaacat aggcgtaatc ttctacttct cttaaaacat
     5101 gcccaaaggc cgtattcgtc aaatcatttc ttaattcttc aggcgattct gctaaagcgc
     5161 ttacaacaag atacgttcct tttgggaatg ggatgattcg cgtagtgttt ggaacatcct
     5221 gactcgtttg aacaccgaca tagtacatca ttttcccgtt atagggttcg ttgactgtaa
     5281 agaaataatg attttcagca gcttgtttca atgcatctat tgttccatta ccgataactt
     5341 tttgctcaaa tgcttgtttc tctgcagcca aaccgacaaa atccgtattg ctactcttca
     5401 attccacacc aatacccgtc accgtaaaat ctggtttttc ttcgatccta tactttgcca
     5461 tttctcccac cctttctatt atattaagtt catcttacat aaaattaaag ggaaatgaca
     5521 cgattacgct cttttttcta gggatatttt tattttttaa ctattttttt gctttcactg
     5581 caatccaaat ctcactgtaa acattactga aatcctctcc ccattttgta aaagagatct
     5641 cgggagcttc gactaaccca taatctgaag aaggaagcca ttctgcatag attttccccc
     5701 aagtatcttg taaagtttgg ggaaatggcc ctttattagg aaaaatggcc caagtgtgtt
     5761 cttctatagc aagcatttct aaatcagcgt acggattttc ttttgtcgta gcaaagccaa
     5821 tcatgtgcat caaactgccc ttttcttcca agcggtctcc atcaaatcca taagaaacgt
     5881 tgattacttg atttggatat aaatctccta acttatgcat ctcttccctt tgagtctccg
     5941 tgatcgtctg cgctaacttt tgtatttctt gattgactcc ttcaaatttg acaggaactt
     6001 ttttcgccac acctgccaca taaaactgtt ctttcttttc gatacgaaat tccatcgatt
     6061 ttcctcctct gacatcaata aaaaatgtca agcgtggaaa agatttttgt atgccgtatc
     6121 tactgatttc tgaaggcaga tatcctgacc aatcacgaaa agctcttgaa aaaccttcga
     6181 ctgtttcata tccatatcta aacgccgttt ctgtcactct tgatccatct agaagatcgc
     6241 gattagccaa agccaatcta cggtttttga tatagtccat tagcgacatc cctgcaataa
     6301 acgtaaagat gcgttttaat tgatattcag agattcctgt attttcggct attattttat
     6361 aggaaagtcc ttctgtcaaa tgttcttcga tgtaatcgat caattggttc agatctttaa
     6421 gcatatttat cttcctcctt gattgccttt ttatcatatt atcaaagaaa gtcaattact
     6481 tgatgaatca ggaaccaatt tagtcagatg tagaaaatgt tgtatggatc taaagaaaat
     6541 tctcaagcag cgtttcgtaa cggataattg gtattatctg cctatctatg ttttaccttt
     6601 agaaaaataa attttgggga agacagattc taagtaaaca aagcggtaaa aatatctttt
     6661 attcccttag ttaatccgct tactacctac tgtacaaagc aaattgttgt tcaaactgct
     6721 gatcagaacg ctgcttggat ttttctttgc gatacatcat acctctttta tcaattggaa
     6781 caattattta tttctaacaa aatcacttca tttttgtcag ataaataaaa aacaaaaaaa
     6841 gttccatctt taaaacacgc tattctttta ctttttctca cattctctaa gaaaagaaaa
     6901 ggagaatgaa tttttgacaa tt
//
LOCUS       DAXWOO010000146         6921 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.149, whole genome shotgun sequence.
ACCESSION   DAXWOO010000146 DAXWOO010000000
VERSION     DAXWOO010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6921
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.149"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            715..2592
                     /gene="ltrA"
                     /locus_tag="ACGE70_002335"
     CDS             715..2592
                     /gene="ltrA"
                     /locus_tag="ACGE70_002335"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFF8693944.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            2723..3100
                     /locus_tag="ACGE70_002336"
                     /pseudo
     CDS             2723..3100
                     /locus_tag="ACGE70_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            3100..4305
                     /locus_tag="ACGE70_002337"
     CDS             3100..4305
                     /locus_tag="ACGE70_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFF8693945.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDP
                     LPFLNSSKK"
     gene            4321..4965
                     /locus_tag="ACGE70_002338"
     CDS             4321..4965
                     /locus_tag="ACGE70_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693946.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPTDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            4976..5782
                     /locus_tag="ACGE70_002339"
     CDS             4976..5782
                     /locus_tag="ACGE70_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693947.1"
                     /translation="MSKKKGERQRFKKRKSYLRWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            5804..6034
                     /locus_tag="ACGE70_002340"
     CDS             5804..6034
                     /locus_tag="ACGE70_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693948.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            6580..6855
                     /locus_tag="ACGE70_002341"
     CDS             6580..6855
                     /locus_tag="ACGE70_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693949.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
BASE COUNT         2601 a         1062 c         1464 g         1794 t
ORIGIN      
        1 aaaagctgtg ggttgcaagt agtctaaaaa ctattgtaat aactcttata acgagaagtc
       61 aaatgagtgg gtaacgccac aaagggcttc cactgatact ccgacatgca acaagtagtt
      121 tatgcagacc aacgttgtgt gaagctcggt gaagtcggct gaaggatacc gtaaggtaaa
      181 agaaatagtg aataccacga aagttctcta gaggtaaagg gtgtatccga taagtcgggt
      241 gtccaaaaaa acttaatatg gtgagtatgt acaaacgtac tgacgaacta gcgaatgtac
      301 tgtccgaaag gcatagctcc tagaaatggg agagacagtg agcaaaaaat cactcattgt
      361 gctaacttgt ggttgagtaa aagtcgttgt tatgaaagac catgctgcgt tacaggcgta
      421 ggagaagtta gggggcaaaa gctagcatct aagttaaggc atgtggatag atataaggga
      481 acaggggaag ccatcaacat ggagagtgca tactcgatga agtggtagta agaaatatgt
      541 gaatgacaaa taacttactt tagtgatggt gaaagtagag tcacgactga tgaaggttct
      601 gtaatggaac tggaggaaca ggctcgattc gtctgtaaaa gtcatgaaac ccgttcaacc
      661 atgggattct cgtatgacta aaaaggtcac gagccaattg gagcgtgata aaccatgaca
      721 aaagaaacaa gtccaaaaga taggaagttg cggcatgcag aatattatgg catgacggaa
      781 atcttcgata agatatactc caaaagtttg aagggagata atttcaagaa tctaatgcag
      841 attatcattt ctgataacaa cattgtccta gcatatcgta atatcaagag aaatcacggt
      901 agtgtgacaa gaggagaaga tggagtaacg attaaagata ttgaaaatct caaccgtaat
      961 gaattcgtag aaattgttag gaagcgattc gcacattata accctcggaa agtaaggcgt
     1021 gtagaaattc ctaaatataa tggtaaatta agaccgttgg gtattccatc aatatgggat
     1081 agaatcgcgc agcaatgcat cttacaagta ttagaaccaa tatgtgaagc aaaattcaat
     1141 aatcacagct atggtttccg cccaaataga tcagcagaac acgcaatagc agattgtatg
     1201 gcccgaatga atcaaagtca catggaatat gtggtagatg tagatatcaa aggtttcttc
     1261 gacgaagtca atcatactaa actcatgaga caaatctggt ctatgggtat acgagacaaa
     1321 cagcttctct taatcattag gaaaatgtta agagcacccg tagtacttcc aaatgggaag
     1381 ttgatatacc caacaaaagg aacaccacaa ggcggaatac ttagcccatt acttgcgaac
     1441 atcaatctca acgaatttga ctggtggata gcaaatcagt gggaggaaaa agatgccaaa
     1501 gagctaaaac atcattttag gaaaaatgga gcttgggata aaggtaatat ctatcacaag
     1561 ctcagaaaga gtacaactct aaaagaaata tacctagtga gatacgcaga tgattttaaa
     1621 attttctgta gaaattataa tgaagcgaaa aaagttttct atgcaactaa aatgtggtta
     1681 caggagcgtc taaagttacc aatatctgaa gaaaagtcaa aaatcacaaa tctttcaaaa
     1741 gaagaaagtg aatttttagg ctttacgatg aaattagtga ccaaaggtaa caaaaaggtt
     1801 gttaatacgc atataggtac aaaagcacaa agcatgatta aagtgaaact aaaaaaacaa
     1861 atcaaacgaa ttcaaaaaag tcctaatagc aaagatacct ttatctatat tggagtctat
     1921 aacagtatgg ttattgggat tcataattac ttcaaaattg cgactcattg tagtgtagca
     1981 atgcgaaaaa ttgcaagaga actcagacca agttttgaaa tacgactacg agaacaagga
     2041 atctcaaaag aaggacaata cggaaagtac gaaggataca agccttacct acggtcagga
     2101 caaatacgat atataagagg gtttccaata ttaccagtag ggtatattca acacagacat
     2161 ccgatgtgta aaaaaagaaa tattaataaa tacaccgaag aaggtaggga agccattcat
     2221 aagaagcaac aaaccgtaga agaatggaag atatcgtggt tgtgtagcaa tccagtgctt
     2281 agtaagagag caacgattga atttgcggac aatcgaattt caaaattcat cgctcaaaag
     2341 ggaagatgtg cagtaacagg tgaggagctt atcttatctg aaatgcattg tcatcatatt
     2401 atcccttatc atgaaagcaa aagtgatagc tatgaaaacc tagtgattgt tactgaggag
     2461 gttcatcggg taatacacgc tacacaaagt gaaacgattg aggaacttct aaaatatcta
     2521 aaattaaatc caaaacaaaa agaaaaactc aatgagttga gattaaaagt tggtaacgaa
     2581 gaaatatctt aataaatatt tgtaggacgg gaaaacagaa tgtcttttat agacgaggga
     2641 acgccgtatg cggtgaaagt cgcacgtacg gtgtggactg ggggaaaaga cggagataat
     2701 atcaaactct tacctatcag tatcgattaa tccagatttt attgaaacgg taaattatgt
     2761 cacaaacttt caacgagaaa tgcgtaaagt agaagcaatg actgttttag ctacacaatc
     2821 cccacaagaa atggtgccag aaaatatgtc ttctgataac ttctcgaaac tgaagaaagt
     2881 ctttgaattt tcttctacga agatctttat gcgtatggat gaatctattt tacgtcatat
     2941 agaaggatta gtgggtaaat caatgacgaa ctcagaatta gaatccattc ctcaacaaaa
     3001 tcaaggagac gctgtgatca attttggctc aaaagaaacc attcgtgttc attttagtcc
     3061 gaacaaacgt caattaaaat tgtttgatgg aggaaaataa tggaaaacca aaatgaaagt
     3121 ttaatcaagc agtatgttaa gcgtcgtgca aagcggcgct tatttttgtg gttgtttggt
     3181 acttctgcag gtttgattac tattttgatc actgtgttcg tgaccctatt tctaatactt
     3241 gcggcgggct cgattgataa ttcggattca gattcaagca gtggtggcga agcttttaca
     3301 ggagaatatt cggaaggctt accgatttat aaagaaatca aaggaagagg ccctttttct
     3361 gatgagattg cgcaatatgc cgtaggtgca gcagtgaaat ataaattatt accaagtgtg
     3421 atcctttcgc aatacggtta cgaatcagcg tttggtacat cggcgtccgc aagaaatgat
     3481 ctcaactatt ttgggattac ttggtttgat ggttgtctgt ttcctaaagg aactgcacga
     3541 ggaattggtg gcattgaagg tggctggtac atgaagtttc ccaactcaaa agctgcattt
     3601 tcctattacg gctttatggt agccacacaa tccaatttta atgcttgtgt aggaaataaa
     3661 agccctggcg caagcttatt gatcttagga cgaggcggtt atgccgctgc aggaattacg
     3721 gaagatagtc cgtattatac aggttgtatg tcaattatca cttctaataa attaaccgaa
     3781 tatgatgaat tcgccatcaa acattggggt gaaggtggca ataacaacgg cacgattact
     3841 ggtgaatgga cgaatccatt ccctggcagc agtttagata aaagttcctt cagcggtggt
     3901 caattatttg gaacgaatcc aggcggtgaa ttccgtccga acggcttcca tgatggatta
     3961 gattttggtt cagttgatca tccaggaagt gagattcatg cggttcatgg cggaaaagtc
     4021 gtgtacgttg gcaatcctgg tatttcaggt ttgggcgcgt gtgtgatcgt gattaattac
     4081 gatggcttga atatggtgta tcaagaattt gcaaatagta caggaaattc tcgagtgaaa
     4141 gtaggcgatc aagtgaaagt tggtcaagta attgggatac gagatacagc acacttgcat
     4201 ttaggattta ctcgaatgga ttggcgtcaa gcacaaggac atgcattcat tgatgatggt
     4261 acatggatcg atccgttacc atttttgaat agcagtaaaa aatagatcag gagaaaaaat
     4321 atggaaaata aaacgttaaa aatcgtctta acagctgcaa ttagcgttag tttaatcggc
     4381 aatattgcgt tgttttggca gctgaaagag gaacaagcag caaataccta taaaattgaa
     4441 gaagtggcta aaaacgcgtc tgagaagctc aaaaaaaatt cctctgaggc acaggcgaag
     4501 atcgacaagt taaatcaaac catcaatgag aaaaaccaag aaatcgatca gctgaagcaa
     4561 caagatggca caaaagcaaa agaggaagta actgaagcac aaagaaagac ggcagaagag
     4621 ttcggtcaat tggccattga taaatcatta agcagaaatg aattaacgga aaaactaaaa
     4681 gatgtcgcaa cccaagaagt gatcgacaaa ttaagtccta cagacgatga tcaccaacat
     4741 gatgattacg gaagttactc ctttacatta ggtgacgtgc aaacttacgc tcaaacaagc
     4801 agtgtaaaag aggaacaaaa ttttgtcgta tttgttgatt atacaatcgg caatccgcaa
     4861 ttcaaagatg tcaaaccaca aaaaataaaa ggtggattaa ctgtcactga aaaacaagta
     4921 gatggtcaat ggaaagtcat agactttagt tatttcactc gttaggagga agaaaatgag
     4981 taagaaaaaa ggagaaagac aacgtttcaa aaaacgaaaa agttatctta gatggctggg
     5041 aatctttatg attctcggct ttttgattct tgctagtccg atttatacga atcagaaggt
     5101 agatatacca gatgaggtaa gcacgaacgc ctttttaaac acgaaagagc tgacaatggt
     5161 ttcaaaagaa aaaggagcga accagcaatt agtttttgaa tttgcgatcg atgatgaaga
     5221 tcaaagcttg ttaaaagaac tggctaatct taattatcgc gtagaagtaa agacagctaa
     5281 aggggattat caagcaatac gagccaaagt gattaaggtg tctgatgatt attttgcagt
     5341 gaagctaacg catgtaccag aagaatatat cgccatgcgt ttaaccatta ttcctgaaaa
     5401 aattgatcca aaagtggata tgcaagaacc acaagatctg atttattacg tgcacgaaga
     5461 taaggttaaa gatcaagtga aagatgaaga ttatgaacaa cacgcgatca attacaaagt
     5521 gaaaggatac aagaaagaac aaaaagcagc tcaaaaacaa atggaatctt tccaagcaac
     5581 cattgatttg aatgaagaat tagtgaagaa gctaaaagat caattgcctt atcaagtcgc
     5641 agatgatcag gaaaatactg aaaacaagat taatagctac cagcaagaaa ttgaaacatc
     5701 gaaaacgcaa ctgaaagatc aagaagagat cattcaaaaa cttcaagaaa aaattgatcg
     5761 tatgacaaaa gaaaagattt agaaggacag gtggagtgaa aaaatgaagc gaacaaactt
     5821 ggctgacaaa gatactttcg ctgagcagaa agtattgatt aaagcctatc tcaaagttgt
     5881 aaaagctaaa gaaatatgtc gagaagtgaa tttatctaac acaacaatat ctgagttgaa
     5941 gaatggtcga aagaatttag atactttaaa attcagatca tttgaagctc tatatgtagc
     6001 tgctaaaaaa tattttacag ctgataacaa ataaaaaaca ggagtatttt atgtgaaaaa
     6061 agtatataaa aaatgcttat gaatcagcta tatcacctta accatttgtt aatgagatgg
     6121 gagaaaacat taaccacgat tctaacgcaa acatcaagca cataaaacat ttaacactcc
     6181 tcctttctgg ctgaagattt caaccaaaca ggaagacagc gtatcaatta tagcatacac
     6241 tttttcttaa attcattttt catatgacaa aatatttcct tctaatcgac aaagttagaa
     6301 ggatttttta ttgtctaaat acacaaataa ccttaatttt taaagagcat aaggaaacgt
     6361 gatatcactt tctcacgaaa ggtgttaggt aggctgtgcc taccacgttt ctaagaaaga
     6421 taaatgatat caaaattttg gatttgtgtg tagatttgtg ggtaggatga aagcgaatgt
     6481 gggcagtaaa ataagcgttt gtgtgtagca aaatcaaaaa atgtgtaggg atatgtgtag
     6541 ctacacatat ccctacacat aataaatttg gaggaaaata tggacttatt aattagaaat
     6601 attgaagaaa agtatattaa taaaattgac aaacgttgtg aagaacttac ggtcaagact
     6661 ggcaaaaaat ggagtcgaaa tcaataccta aaagttttga tcgaaaatga ttttgatcac
     6721 gctcttctga attataaaaa agaccaattt gatcggttgt tagaaaaatt tgtggatatt
     6781 caaacttata atacgaaaac tttagaagaa tatattgcaa caaataatca attaattggt
     6841 ttgttaattg aataataaaa atagtattgt gaatttataa aaggaggaaa tcaattttgg
     6901 caaaagcaac aagaaaacat g
//
LOCUS       DAXWOO010000147         6635 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.150, whole genome shotgun sequence.
ACCESSION   DAXWOO010000147 DAXWOO010000000
VERSION     DAXWOO010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.150"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            41..778
                     /locus_tag="ACGE70_002342"
     CDS             41..778
                     /locus_tag="ACGE70_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFF8693950.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            792..1607
                     /locus_tag="ACGE70_002343"
     CDS             792..1607
                     /locus_tag="ACGE70_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFF8693951.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            1620..2267
                     /locus_tag="ACGE70_002344"
     CDS             1620..2267
                     /locus_tag="ACGE70_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFF8693952.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            2283..2939
                     /locus_tag="ACGE70_002345"
     CDS             2283..2939
                     /locus_tag="ACGE70_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFF8693953.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            3101..3922
                     /gene="racE"
                     /locus_tag="ACGE70_002346"
     CDS             3101..3922
                     /gene="racE"
                     /locus_tag="ACGE70_002346"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFF8693954.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            3925..5280
                     /gene="rph"
                     /locus_tag="ACGE70_002347"
     CDS             3925..5280
                     /gene="rph"
                     /locus_tag="ACGE70_002347"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFF8693955.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            5281..5799
                     /locus_tag="ACGE70_002348"
     CDS             5281..5799
                     /locus_tag="ACGE70_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFF8693956.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            5906..6406
                     /gene="cbpB"
                     /locus_tag="ACGE70_002349"
     CDS             5906..6406
                     /gene="cbpB"
                     /locus_tag="ACGE70_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFF8693957.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         2175 a         1114 c         1465 g         1881 t
ORIGIN      
        1 taaaagaaat cttactaaat aataatggga gtgtaattct atggcaatga ttaaatttga
       61 tcacgtggat aaatattatg gcaagttcca cgcattaaaa aatatcaatc tcgaattcga
      121 gaaaggagaa gttgtcgttg tcattggtcc ttctggttca ggaaagagca cgatgcttcg
      181 ctgtatcaat ggattagaga cgatctcttc aggtaagctg ctgattaatg atactgactt
      241 gcatgataaa aagacaaaac tgacagaagt ccgaaaaaac atcggcatgg ttttccaaca
      301 tttcaatttg tacccaaata aaactgttct ggaaaacatc aaattagcac caatcaaagt
      361 gttgaagcaa gaccaggcaa ctgcagtcaa aaacgcggag aaatttttga aaactgtcaa
      421 tatgttagat aagaaagatt cttatccttc catgttatcc gggggacaac agcaacgggt
      481 ggcaatcgcc agaggtttag cgatgaatcc tgagatgctg ctgtttgatg aacctacttc
      541 tgctttggat ccggaaatga ttggtgatgt attagacgtt atgaaaaaat tggctcatga
      601 cggtatgtca atgattgtcg tgacacatga aatgggcttt gccaaagaag tggcggatcg
      661 tgttatcttc atggctgatg gagaagtatt ggaagacagc agaaatgtga gagacttttt
      721 cgaaaatcca aatgaagtcc gagcacagca atttatcagt aaagttatca atcattgata
      781 ggaggaaaaa gatgcgcagg ttaaaaaaaa tacgcctgat tttcctttta ggcattgctt
      841 ttcttgcact agccggatgt aaaggaaatg ggttggcaaa tgaagatatt ttagagcgaa
      901 gcaaatctac caatgaaatc atctggggtg tcaagtatga tactcgatta tttggaatga
      961 tggatattga gagcagaact gtacaaggat ttgatgtaga tattgcaaaa gcaatcacta
     1021 aaaagatcct tggtgacaat ggaaaaacag aatttgttga agtcacttct aaaactcgga
     1081 ttccattatt gaaaaacgga aatatcgatg caattattgc aacgatgacg atcacagatg
     1141 aacgaaaaaa acaggttgac ttttcagatg tctatttcga tgctggccaa gctttgttgg
     1201 taaagaaagg aagccaaatc aaaagtgtag atgatttgaa tgcttctaca acggttctag
     1261 cagtcaaagg ttctacatcg gctgcgaata tccgacagca tgcaccagat gcaaaaattt
     1321 tagaattaga aaactatgca gaagcattta ctgctttaca atctggtcag ggagacgcga
     1381 tgactacgga taatgcgatt ttgttaggga ttgcagatga aaatccagag tatgagttag
     1441 ttggtggaac gtttactaat gagccttacg ggattgcgat caacaaaggg caagaaaact
     1501 tcttgaaagc tgttaaccaa gcgctagagg aaatgcatgc agatggcaca tacgataaaa
     1561 tctatcaaaa atggtttcca aatgaaactg aaggaaaagt agagtaaggg ggagaggcaa
     1621 tgagcgaatt gatacaaact tatggaccaa tctttgtcga cgggttcaag atcacattgt
     1681 tttcgagtgt cttagcactt gtgtttagtt tgattatcgg aacgttaatg gcaatcttcc
     1741 aattatcaaa gaataaagta gtgagcgcct tagcaaaggc atatgtagaa ttttttagaa
     1801 acattccatt attaatcatc gtgatgtttt tttatgttgt cgtaccgatt tattggttta
     1861 atctagatgg attccaagca ggtacgattg gattaacgat ctatacatct gcttttattg
     1921 ctgaaacagt acgttcaggg attcaaaccg taccaaaagg tcaaacagaa gcaggactat
     1981 cttctggttt tacctattca gaaatcatgc gttatattgt tttaccgcaa gcattcaaaa
     2041 tcgttatccc gccattaggc aaccaattta tcaacctcgt aaaaaactca tctattttag
     2101 caatcgtagc tggtttagat ttgatgtatc aaggagactt gatcgctagt gaaacattca
     2161 ataccttcga tacgtatatc atcgttgggt tattttactt agttatcacg ctaccgttat
     2221 cttacttaat ggtttatctt gagaaaaaat gggcggttcg ttcatagaag gaggggaaat
     2281 acatggattt tagcggcgct ttttcatgga tgaatatccg ttttttattg gaaggattaa
     2341 aggttactgt tgaagtttca ttgttttcga tcatttttag tttcttgatc ggtggtctga
     2401 caggtgtgat tcgttttgcg aatatccctt tcttttccaa aatccttggt gtagtgattg
     2461 atattatacg gaatcttcca ttattattga tcattttctt cacttacttt gccttacctc
     2521 aaatcggcat tcagatgaac atcttctggt ccgctgtcgc agcattaacg atttttgagt
     2581 cagctatgtt gtcggaaatt ttccgagctg gactcaatgc tgttccaaaa ggacagatgg
     2641 aagcaggatt atcaacggga ctgacttatg tagaatcgat gcgaacgatc gtgttaccgc
     2701 aagcatttaa atcaatgatc ccggcaattg tgagccaatt gatttcactg atcaaagata
     2761 cttcattagc agtaatcatt tctttacctg aactttctca tcaggcacgt attatttatg
     2821 gacagaacac caattatgtg cttccgatgt tcatcatcat gacacttatg tacttcgtgg
     2881 tatgttacgc gctttctctg ctttccggtt acttggaaaa gagacggtac agttattaat
     2941 agatgattct tttagcggtt gtggcaaact aaagtcacaa ccgcttttcc tctttccatt
     3001 cgaaaaaaag agatttcgat tgttttaagc aagtaagagc acttgaaaat tcacgctaaa
     3061 aagtacacaa tagggaggat agaatttatt gaaaaaaagg atgatacgat tgacagataa
     3121 tcgccctatc ggatttattg attcaggtgt cggcggcttg actgtagtaa aagaagccct
     3181 gaaacaatta ccgaatgaaa atattttatt tgtaggagac acagcacgct gcccatatgg
     3241 ccctagaccc gcggaacagg taatccagta tacttgggaa atgacggatt atctggtgga
     3301 gcaaggaatc aagatgctgg tgatcgcctg caataccgca actgcggtgg ctttagaaga
     3361 aatcaaagct gctctttcta ttccagtcat cggtgtgatc cttcccggta ctagagcggc
     3421 agtaaaaaaa acacaaaata aacaagttgg cattatcggt acgattggta cggtaaaaag
     3481 tcaagcttat gaaaaagcac tgaaagagaa agtaccagaa ttgactgtga caagtcttgc
     3541 ttgtcccaaa tttgtttcag ttgtcgaaag taatgaatac cattcatcgg tggcgaaaaa
     3601 aattgtggca gaaacattag ctcctttaac cactaaaaaa atcgatacat tgattttggg
     3661 atgcacccat tatccattat tacgccccat cattcaaaat gtaatgggag aaaatgttca
     3721 actgatcgat tctggagcag aaacagtagg tgaagtatct atgctgttag attatttcaa
     3781 tttgagcaat tcaccgcaaa atggtcggac attatgccag ttttatacaa ctggctctgc
     3841 caaacttttc gaggaaatag ctgaagactg gcttggaatc ggacacttaa atgtagaaca
     3901 tatcgaattg ggaggaaaat aaggatgcgt catgacggga gaaaagccca agagcttcgc
     3961 aaactggagc ttcaaacaaa tgttttcaaa tatccagaag gatcggtagt gatccgtttt
     4021 ggtgatacaa cagttatttg ttctgcaaca atagaaaatt ctgttccccc ttttttgaga
     4081 gaaacaggaa caggatgggt gacagcagaa tatagtatgc tgcctcgagc aacgagtacg
     4141 agaaatcgta gagaaagcag caaagggaaa ctttctggta gaacaatgga aatccagcgt
     4201 cttattggtc gttccttgcg tgcagtagta gacttggaaa aattaggaga acgtagtatc
     4261 atcgtagact gcgatgtgat ccaagcggat ggcggtacta gaacagcaag tatcactggg
     4321 gcatttgtgg cattgaaatt agccattgaa aaacttttaa gagaaaaaga attgtcagaa
     4381 gatccaataa aagaacatct tgctgcagtt agcgtaggaa tcttaccaga tgggacttgc
     4441 gtcacagatt tggattacca agaagattcc gcagcacttg tagacatgaa tcttgtaatg
     4501 acagaatctg gaaaattcgt ggagattcaa ggaactggag aagaagcaac gtttgatggt
     4561 gaacagttga atgaaatgct cttttttgga aaaaacgcga ttgaagattt gatcaaagag
     4621 caaaaacatg ctttgctaac agagtttgct caaaatgatg agcggataga agaaacaaaa
     4681 acaatcatca ttgcgacaag aaatccagga aaagcagaag agtttcgaaa tatgttcaaa
     4741 gaagcgggat atcatgtaaa aacattactt gactatcctg aattgccaga tgtggaagaa
     4801 actggcagca catttgaaga aaatgcccga ttaaaagcag aaacgattgc acaattgctt
     4861 gatcagccag tcttagcaga tgattcaggt ttgaaagtgg atgctttagg cggtatgcca
     4921 ggaatctatt ctgcacgttt tgcaggggaa caaaaaagtg atgcggggaa taacgccaaa
     4981 ttattatatg aattgacaga tgtgccggat gaaaaaagaa cagcacaatt ccattgtacg
     5041 ttagtattcg cggcacccaa aaaagacagc cttgtagtag aagcggaatg gcccggtcga
     5101 gtagcccgta taccatctgg cgaaaatggc tttgggtatg atcctttatt tatcccagaa
     5161 ggtaagaaac aaacagcagc agaattatcg agcgaagaaa aaaacaagat cagtcatcgg
     5221 gctcaagcaa tgaaaaaatt gagtgccgag tggaaacaat ggctggaagg agagagatag
     5281 atgcggtatt tagtagtaag tgataatcat ggagacagag atatcctcgt tgacttagta
     5341 gatcgttatc gttctgaagt ggatatcttt ttccattgtg gtgattcgga attagaagct
     5401 tctgacccat tatgggatac attccaagtg gttcaaggta attgtgatta tggaccagga
     5461 ttcgaacaga aaaaagtgat ccaaactggt caggatacag tattcatgac tcacggtcat
     5521 ttatcgaatg tccgatttgg tttgacacag ttagcaatcg aagctcgagc tgcaactgcc
     5581 gatatggtgt tttttggtca tacccatcaa ataggctgtg agatggatca gaaaatcctg
     5641 tttttgaatc ctggtagcat ttctcagcct agaggcccga tccaaatccc atcatatgct
     5701 cttatcgaaa gtttagatga ccaaatttct gttcaatact ataaccgtgc ccatcaggca
     5761 gttgaagagt tggccttttc tttttcgaga gagcgctgaa taaaaaaact ctaattttta
     5821 ataaaaataa gctttatttt atgaattttg catccggttt cggtaaaata gtagtgttga
     5881 atgaaataga aatggaggcc atcagatgat tggacctata gtaagagaaa tgctgctgga
     5941 aaatcaggaa acgtttttga ttcctgcaga aaatgtggca aatgttatgt atcatcatcc
     6001 attgtcccat ggtttacttg tgttatcgaa ggtcggttat tccaaaattc ctgtattagg
     6061 aaaagatgat cactttgtag gactagtaag tttatcagat gtggtcaata aaatgattga
     6121 cttgcaaaca atcagcatgg acccgttaga aggactaacg gttgctgatg tgacagaaac
     6181 agatgttccg acaattgatg aaaattggga actagaagaa gtactccatt tgttagtcga
     6241 ttcgcctttt ttaccggttg tcacagaaga tcggattttc aaaggaatca ttacaagaaa
     6301 agagctgctt aaagccgtaa acttcatggt gcatgaatta gaacgtcgta atatattgtc
     6361 tccgaaaatc gaagaattaa aagaacaaat cgatatcgtg agctaaagaa cgagaaacta
     6421 aaagataatc aaaaaataag tagaaaaaac agcgtaccag ttttgacttt atcttgaaaa
     6481 agaggctggg acaaaagcgt ttaactccaa gaaataagaa ggaattcacg aaaattgctt
     6541 ttcaaatttt tgtgaatttc agcttatttc cgaaggagtt gcttctgctt ccgccgttta
     6601 tacgtgttta gagcgtggga caaaagtgtt cttta
//
LOCUS       DAXWOO010000148         6551 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.151, whole genome shotgun sequence.
ACCESSION   DAXWOO010000148 DAXWOO010000000
VERSION     DAXWOO010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.151"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(1209..1874)
                     /locus_tag="ACGE70_002350"
     CDS             complement(1209..1874)
                     /locus_tag="ACGE70_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8693958.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDF
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2054..4036
                     /locus_tag="ACGE70_002351"
     CDS             2054..4036
                     /locus_tag="ACGE70_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HFF8693959.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERVISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4040..4678
                     /locus_tag="ACGE70_002352"
     CDS             4040..4678
                     /locus_tag="ACGE70_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8693960.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4706..5134
                     /locus_tag="ACGE70_002353"
     CDS             4706..5134
                     /locus_tag="ACGE70_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693961.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            5591..6502
                     /locus_tag="ACGE70_002354"
     CDS             5591..6502
                     /locus_tag="ACGE70_002354"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFF8693962.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
BASE COUNT         2261 a          988 c         1209 g         2093 t
ORIGIN      
        1 cagattttga ccagtctaat ttacattcta atatattcat atgagctaat tttttgatta
       61 aatggtaagt ttttccttgt ttgttttagt ttaaactagt gtgtaattta aaaatctata
      121 tggaagggtc tgtggggtaa tacttaaata aaggctagga acctgataac atcaaggttc
      181 ctagcctttt tataataaaa tgaagcgaaa ataatttctt ttaaagagcc gagattatca
      241 gctatataag agtgaatttg atgcaaaaaa cagagccgaa acagtcttgg tgttcgtagc
      301 tctgcttata tgtttcgaat ttaagacaaa gagttggcaa aagatgttga taaggaagat
      361 aagataactg tgttatataa actaggtaac taactgtaac aagctaattt taagagattc
      421 ttattagaag acgtttagga atataactaa gaaaatgatt atacaggtaa ttgtcatttc
      481 aataatgttt tctaattttc gcgtctttgt tttaagaaaa aggccagaat cagttggaaa
      541 aaagttcgta cgatagtgaa gaggggcaaa atttcctact aatattccca ctaacaagag
      601 ataaaacggc caggttggtg tgattcctct ataaaaaaat gcccatgcta ccgaaatccc
      661 atagattaac aactgaacaa tttttacaat gaaaaaattg ttctgccatt actcaaagtc
      721 tcttcctcct tgtatcgttt atttgtttca ttttatcatt atgataggcg cttgaacaat
      781 ccattttgta ttttattgag tactcttgga taaactacca gcgattatca tatttattta
      841 tgttactgta agccatcata taaaagtaaa tgagcagatc atagaaatat aaaaaagaac
      901 taaaattgat ctaaattatg tgcagcttga ctacgtacca atctatatta cttactagat
      961 tggtcgttaa tctattcgga tcgaatgagc aaaatatttt tgtgaatgtc catccttgat
     1021 tatgattcca aaaagaatca ttgggaggct gtggcgtaaa tattatacta taagccagaa
     1081 atacctatca ttctgaactt ttttatttag gaactttcat ctcaaggctt tttcaattaa
     1141 tttgtacccc tgttttttgg ataagatcat gtgcattgat tttttctatc tctacgttca
     1201 tctcctactt aatgcctatc tggctcctga gggcttcttt ttaaggcgtc gtcatagtct
     1261 tggctgagga tttcttggta atctgaggga atggcttccc aagtgagcag gcgaacgttc
     1321 atgacgtgtt ttcctttgtg tttgattaca gcatggtttt tctctttttc tacctctcca
     1381 gcgtgaaaag acagccatgt accatggcca aagatatcat tacataccca acgttcgatg
     1441 gaattgtcca cgtatcctac agggagtgtg gtatagccga tttgtggttt caaaaaagga
     1501 ttgaatcgat cgatcgtttc atttttgtat tgataattga taagaaaaag atagaccaaa
     1561 caaaagacga gcagaaagaa agatagtgtc tttttgagtg agaagggttg ttttgatgaa
     1621 taagatttct tgttaacaag aggttcatct ggcaatgttg gttctattga ttcggtagct
     1681 gattcaatag aaggaaaatc aatcggcaga cctaaagtat gatgtaaaag tgacagcatt
     1741 tcaaagtctg gcattgtacg agaattttcc caactagaaa tcgttttatc ggaaacacca
     1801 attttttcgc ctaattcttt ttgcgtaagc ttacaatttc tgcggtaagt tcgtattttt
     1861 tctccggtgt tcatcttctt ctcctttcga ttctacagta tatagattta ttctacatga
     1921 cctatacact tgatagatac gtttttttga tgaaaaacgg tatgatttta tcaaagaatc
     1981 atttaagtaa gggagagtgg tgataatcaa aaaaaagttc acagttttac tcacaatcat
     2041 agtgaccggt atcatgagtg gttttgcctt ggttagtata aataaacagg tactctatcg
     2101 gacaaatgaa tttttccatc aaacagatac ttatactccg attcgattgc cacaacattt
     2161 tattgggcaa gaagaatatg agcagctaat catggtattt gaaaatgtct catcagaaac
     2221 tgagctttct tatatgaaaa aaacagtaaa tgaatccaat gaagtagata catcttggaa
     2281 cttcaagata cagccaaagc ttgaaatgac attgtattat caaggggatg tcaaatcttc
     2341 tcacgagaag gtaccctatt ctaatacctc ctttcgcgta acgtccattg aagaatcgat
     2401 caaaataaaa gattttgaag gtgaattttt cttgatgacg agagaaaaag aagcctatga
     2461 acgggtcatt tcactattca ctgatcgatt caatcaagca tttcacactg attatgccac
     2521 taatattttt gaagactttg aaaatagcta ttcgtattcg gttatttcaa atacagacgt
     2581 atataattat cgagtgtatt taactattgg attgattctt ttattccttt tgttatcatc
     2641 ctttttctta ttgatgaata aagaaatcag tttattgttg aataatgact tttcattaca
     2701 tcaagtattg tggtgtttat tggggaaaaa gaaaatcatt attacattca taattgcagg
     2761 aatcattggt ggttttattt ttatgtatta taaggagcaa tttctttatt tgttccagat
     2821 gtacgttttc ttgttcagcg gaatatatgg tatctctttg atttctcttt tattagttga
     2881 aaaaatcgct tcgttaagag aagcgttcaa ctatatccaa tacattcttt ttacatttgg
     2941 attgttgttt tctcttcttt tgaccgttgc caatgtcgaa ttagcaacta tcgttttttc
     3001 ttcttcacag ttagctgttt cccatgaacc accaaaagaa atcgctggtg aggactatca
     3061 tgtattttat ccagtaacca tcgggaaaaa ccacgtggat tttatttatt ccaatcgttt
     3121 tgcatcagca gcagatcagg aattatatga aactttgaat aaaaacggaa gtatattagt
     3181 caatgtatca gattacttga atgaagaaaa tgaacagttt ggcagaggga taaaaataaa
     3241 tctgaattat cttaagaaat ttcctttgat tgatgtctct ggaaggttga tccaaataac
     3301 cgaaaaagaa acgcaccctg ttatattgat ccctgaacga tatagacata cagatttaaa
     3361 aaatgacctg gcgcaaataa cagaatatta tcaagaaatc agtgaaaaag aaccgaaatt
     3421 tctatttata aaaaacaatc aacccattta tacatttatt ccttctatcc cctggatcga
     3481 acattatccg gttgttgaaa tactgacatt gaaaaacagt atgtatttgg aaagaaatat
     3541 tctcagcgga gaaatctatc caccgttaaa gatcaagata ggctctttgt cgaaagaaag
     3601 gttatttgag ctgattgaag aaagtcagtt acgtgataac ttgcagttat catttccgta
     3661 tactcagact gaagagatag ctgtcaaagt gttaagcaga tcatttcatt acttgataca
     3721 gatattttta ctcactttct tttctttctt cttgttgagc tatgtcatgt tatgcatata
     3781 ttttgtctac aattgtcgga aaatcgctat ctttcgttca agtggttatt cgctctttga
     3841 aacgtataaa gatttcttca tgatgaacct catcaaatgg ggaacgacaa gtgtaatctt
     3901 cctttttctt atagaaagag agcctaaata tctttttaat atttttttct ttagcttcat
     3961 tgattttatt ctttcagttg tttttatttt gtctacagaa aagaaaagtc agttattgct
     4021 gatgaatgga ggttaacaat tggagccgtt gatagtgata aaaggtgcga aaaaaatcat
     4081 tcgaaacaaa acgattcttc atttggatcg tttcactgcg gatgctgggg atcttgtgac
     4141 tgttgttgga gaaagtggag caggtaaaag tacgttattg aatatattag gattgaatga
     4201 tcagttcagt tcaggacaat tctatttgtt cgatcaagag acgaaacaat taactgggag
     4261 agagaagcgc ttgataaaaa gaaataccat atcttttctt tttcaagatt ttgggttgat
     4321 tgaatatgaa acaatcgatt ttaatttaga tattggattg aagttttcga atttaaatgt
     4381 ttcagataag cgaacagcaa agtatgaggc attgaaaaaa gtaaatataa acaagaagct
     4441 aagtacgcct atctacacat tatctggtgg tgagaagcaa agaatcgcct gtgcgagagt
     4501 cttattgaag aatagcaaaa tcattttagc cgatgaaccg actgggtcat tggataaaag
     4561 aaataaggat aaagtagttg aaatcttatc gcaacaagct tcccaaggta cgttagttat
     4621 cgtagtcacg catgatccat atctgaccaa aataggaaac aagaagatca tgctttaaaa
     4681 atcaaaaagg aaaggatgtc gttttatgaa aaaagcagtt attatttgtg cactagggat
     4741 aggttttgtt ggaggaaacg ctatgtcatc tgaagtggaa gcaaattcag ttcacggtga
     4801 aatctattac aaatctttaa agccaggaga gattcatttt gatgctaaag gtaatattat
     4861 taagtcaaat ttaccagtag accgggaaat ttctagaaca gcaattaatc atagctctgg
     4921 aagatgggtt tattatagtg atacaacttg tacgagaaaa acaggtcatt ctaaccacta
     4981 tagtcgtgtc tataatcgtc acggtgcaaa agcgaaagtt ggaaactcta ttcgatcagc
     5041 aaatgcaaca aaaggagtat gggcctatgc aagtgcgaca ggaaatagaa ctgacacatt
     5101 ctcatgttgg tacaatccaa ccggttggta ttaagtatcc aaaagtttat ataaaataga
     5161 aaaaacatat aatgaaacag cgttaaaagt tatttttggt agttttaata tattcatttt
     5221 tagttgtata aaaatatatc ataataaaaa caacaaaaaa atgtctggaa aatcgaacat
     5281 aaaagtgtct gatttgaata tcccagcagc ggaagaagaa aaacaaaatc gataacattt
     5341 gttctaagaa ttagttacgt taaatacgga acgcaagata gacttgaaaa acggtaacaa
     5401 aagaagtagt atattaaggt gtgatgtgct acttgacatt tattctgctg agcgaacttt
     5461 ctggattgtg tagccttata tctattcagg acgtatcgag cagattagta aaggtgttct
     5521 tgatgatttt catcttgaac acctttgttt ttagcaattc gcaaaaaacg aaaagtagga
     5581 gaattgataa atgaaaaaaa gatttttagc acttgccatc gtactaggca ctggcttatt
     5641 gtcaggctgt acgaatgccg gtgaaaaaac agccgttagt tataaaggcg gtacaatcag
     5701 cgaacaagaa gtaatggata gcttgaaaaa aatgcaagga gcagattccg ctgtgcaaca
     5761 gttgatcgtt tatcaagtat ttgaagataa atatggagat gacgtatcaa ccaaagaaat
     5821 cgattctcaa tatgatcaaa caaagaaaca attgggtgat tcatttgatt cacaattgaa
     5881 atctgcaggt tacacagaac aaacattcaa agacagtatc aaacaaagtc tagcctttca
     5941 agaaggtcta aagaaacata tcaaattgac cgatgaagat ttaaaaacgg cttgggaatc
     6001 tttccatcca gaagtggaag ctcaaattat ccaagtagca agtgaagatg atgcaaaaga
     6061 tgtgaagaaa gctgctgata agggagacga tttctctaaa ttagccaaag acaagtcaac
     6121 tgatactacg acaaaagaag atggcggaaa agtgaaattt gattccacta ctacaacggt
     6181 tccggcagaa gtcaaagaag cagcctttaa gttgaaagat ggtcaagttt ctgatgtgat
     6241 cacttctacc aatgcttcaa cttatactac ggaatattat gtggttaaaa tggtgaaaaa
     6301 ccaaaacaaa ggcaacgaca tggataaata caaaaaagaa ttgaaagaaa ttgcaacaga
     6361 cacaaaatta agtgacagta ccttccagaa taaagtaatt ggcgaagtat tgaaagacgc
     6421 taatgtaaaa atcaaagaca aagactttga aaatgttttg agtacattca cttcagattc
     6481 tagtacagca agtagtaaat aagcggtata aataaaaaat atgaactatt taaatcaata
     6541 aaactgttca t
//
LOCUS       DAXWOO010000149         6450 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.152, whole genome shotgun sequence.
ACCESSION   DAXWOO010000149 DAXWOO010000000
VERSION     DAXWOO010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.152"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            177..515
                     /locus_tag="ACGE70_002355"
     CDS             177..515
                     /locus_tag="ACGE70_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693963.1"
                     /translation="MFDSIKNLVSGMTPDMKTLVQVVLYFLAFVFSLKPGFTAMKFFG
                     DAKWGDGLKYVGAIIAIFIIPVITTVMLYTMGTKTGNDLNQKANIISALIPVVGIYLV
                     SKFPKNELLG"
     gene            625..846
                     /locus_tag="ACGE70_002356"
     CDS             625..846
                     /locus_tag="ACGE70_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693964.1"
                     /translation="MVFDREKMLAHANEVLMSSLKGTELAKIMNMNVNQFYDYRNGSK
                     KIEKARLETLIKFEKAYVYMLDKQKRTID"
     gene            866..3022
                     /locus_tag="ACGE70_002357"
     CDS             866..3022
                     /locus_tag="ACGE70_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693965.1"
                     /translation="MAKFKDITESINYVAETVPVVALSLKPQLLEVYKKEEKEAKAKK
                     AKAKVWKEFDALLTKDLPKKNYMVIEDVQKLNSEGVIDEDDAILDSEKYVVLLKSSLN
                     YVADLPEKFKEKLLAGVRFMDSSGQLLVIPDDPNVFESEVEEATDFYSAVEQLEVATN
                     GLERIAETFNSSEEVEFEAVAENVPDEKEALFVDDNKPTEAENQEVSIEETQIQTETY
                     QQPKIDEKPESQSDKENYVYEAEKITSESDVPKNLDADFLEKNFEQIIADKFQPVVLD
                     EINDPVDLNEEMLSIPELKPLLFLAKESMNARISIANNKINSELNKTKEQLILKGKQM
                     LADELLRIEEATSLNGQNQFTNTLSVAKESYEAEASKIDDLVMEKSNIIRKDYDMAKQ
                     AYLDAELKKLEQRYDAEHLPEVKQQEDEYRKATEESLDQQYQEAIESIKNDALKQSQE
                     AKEVVVERIINSLKPEIEKSLTDVQEKTLEIVEKTKSENEAEFHDTKEKTTDVVVKYF
                     ANKEEVEKQVANNTKELETKLVEQQKTIEEYKDRMKEVKDLAEGYKLNSEKLAEQSEA
                     LREDLSRSRSDLAQMAEKTKSLAVATENQSSSKRDFTSKKMATIISVAAMACGTILGS
                     SYFISHNNQQAQAQTQTSISRVENSIGTNTNSASTNSSYEATPTTEFKVGKDVVISID
                     GKTTKAEVISIDGEKAVVKTPDGNQYKIQAHLSDNN"
     gene            3034..3267
                     /locus_tag="ACGE70_002358"
     CDS             3034..3267
                     /locus_tag="ACGE70_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFF8693966.1"
                     /translation="MREEKIVYPVIQNNQENMTSSQHLQEAQLVYQVNELPQTNETMS
                     KSISLIGLLIVAIVITVGVYRKLNVKGSISDGR"
     gene            3257..4123
                     /locus_tag="ACGE70_002359"
     CDS             3257..4123
                     /locus_tag="ACGE70_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693967.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVEWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLKFIFVPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNSARVSMGKKKPFRGTEILKVKQDGTIVYRDGSVALMFNLDGATSLTAFP
                     EEVMAQESKSIQYQNTRDRSLAEIKITSSQPQNTERQTRNMKIQKRNARSNKAVFDII
                     DQQERFVSEYIDGVKTTVIQYLILKSQSKMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSDIYALK"
     gene            4138..6108
                     /locus_tag="ACGE70_002360"
     CDS             4138..6108
                     /locus_tag="ACGE70_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693968.1"
                     /translation="MEIMGRSQLLNFKKKRKTKKEQKTRNSKVVNNTFVSDLTSLVGY
                     QFTPSYIRTGKRWGTIVKLVNTYGMNRNYGFGWMVKAIPEVRVEGVKAYLIEADKPVS
                     QKDQQEIMHENVGGSTNSYENNLEGKNSDAEISKTVMRIMDLNTASLLDSMANKIIDW
                     KLEILLVSDSPNKISEQLIKLQRLYDEKMYGLKLMSYGGDQRDLFDRLLSEPIGNVYE
                     YTSMSSDFAGNDHAIRKGLDDANGWAIGTMSTSYASGVAFMALDDSFTSKVLVASHKN
                     ATIKGYDEKISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGRTFVAKPSLNS
                     VIEKVNLAEEGLNSLELFGNQKDIGDLYRRNLDKLTQIFYLLAGRTLDSATRVLLKKE
                     LGNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNAETKAKKSGTEKEVDRI
                     IVLKDTLRTALDSFSRMFNQRTTLKEIGKSDKLQFYYNLGDLRAETNVMEAQFLNTFD
                     FITSQAKKDDIVMIHGFDKLSIETVGLIKKRLTMLEEDGIRLAFLFDTIGSGEDNEYK
                     EDIETADIFNTDGKIYQSFESDFNYTIIGKMTSGELMKYEKKVAPARKGEDDQKLSVD
                     LRQYLTTGRSNKFQIRRPVDLTNNFVIGEFIL"
BASE COUNT         2482 a          881 c         1256 g         1831 t
ORIGIN      
        1 gtgaaataat ttaatggaaa atctctttta atcatcgaaa atttatgtta ttatgataac
       61 ataaagatgt aaatatcgag acgtcttttt cttagttaga tcattgacaa gtgaacaaat
      121 aagatttgtc gatgatataa ttaacttatt aaaaatatta aaaaggaggt ttttatatgt
      181 tcgattctat taaaaatttg gttagcggaa tgacgcctga tatgaaaact ttagtacaag
      241 ttgttttgta tttcttggca ttcgtttttt cgttgaaacc aggattcact gcaatgaaat
      301 tttttggtga tgcaaaatgg ggagatggat taaaatatgt tggggcgatt attgcaatct
      361 ttattattcc tgtaattaca accgttatgc tttacacaat gggtactaaa acaggtaatg
      421 atttgaacca aaaagctaat atcatatcag cacttattcc agttgttggt atctatctag
      481 tttcaaaatt tccaaaaaat gaactgttag gttaaaagtg aattgaacaa atttggataa
      541 tatgccgatt tgttcaattt actttttacc aaaaaactac gctccaagcg tagtttagaa
      601 gtgagggaag aaagtaaatg taagatggtt tttgatagag aaaaaatgtt agctcacgcc
      661 aacgaagtat taatgagcag tctgaaaggt accgaactgg caaagataat gaatatgaat
      721 gttaaccagt tctatgatta tcgaaatggg agtaaaaaga ttgaaaaagc cagacttgaa
      781 acgcttatta aattcgaaaa agcatatgta tatatgctag ataaacagaa aagaactatt
      841 gactgaaaaa aggagtttta caataatggc aaaattcaaa gatattaccg aatctattaa
      901 ttatgtggca gaaacagttc ctgtagttgc actctctttg aagccacaat tactcgaagt
      961 gtacaaaaaa gaagaaaaag aagcgaaggc aaaaaaagct aaagcaaagg tatggaaaga
     1021 attcgatgcg ttgctgacca aagatttacc aaagaaaaat tatatggtaa ttgaagatgt
     1081 acaaaagctc aacagtgagg gagttataga tgaggatgat gctattctcg attctgagaa
     1141 gtatgtcgtt ctattgaagt caagtttgaa ttatgtagct gatctaccag aaaaatttaa
     1201 agagaagcta ttagcaggtg tccgttttat ggatagtagt ggtcaattat tagttattcc
     1261 ggatgatcct aatgtgtttg aatcagaagt tgaagaagct acagacttct atagcgcagt
     1321 agagcaatta gaagtagcta ctaatggatt agaacgaatt gctgagacct tcaactcttc
     1381 tgaagaagtt gaatttgaag ccgtagcaga aaatgtacca gatgaaaaag aagctctttt
     1441 tgtggatgat aacaaaccaa ccgaagcaga aaaccaagaa gtaagcatag aagaaacaca
     1501 aatacaaaca gaaacctacc aacaaccaaa aattgatgaa aaacccgaat cccaaagtga
     1561 caaagaaaat tatgtttatg aagctgaaaa aatcacatct gaaagtgatg taccgaaaaa
     1621 cttagatgct gattttttag aaaaaaattt tgaacagatc attgctgaca aatttcaacc
     1681 tgtagtttta gatgagatta atgaccctgt cgatttaaac gaggagatgc tatccatacc
     1741 tgagttgaaa ccattgctgt ttttagctaa agagagtatg aacgctcgca tctctattgc
     1801 taataataaa atcaacagtg aattaaataa gacgaaggag caattgatcc tcaaaggaaa
     1861 gcaaatgtta gctgatgagc ttttgcggat agaggaagca acctcattaa atggtcaaaa
     1921 tcagtttaca aatacactat cggttgctaa agagagctac gaagcagaag catctaaaat
     1981 tgatgattta gtgatggaaa aatccaatat cattcgtaaa gattatgata tggcgaaaca
     2041 ggcttatttg gatgctgaac taaagaaact agagcaacgg tatgatgctg agcatttacc
     2101 agaagttaaa caacaagaag atgaatatag aaaagcaact gaagaaagtc ttgatcaaca
     2161 gtaccaagaa gcaattgaat ctattaaaaa cgatgcattg aaacaaagcc aagaagcaaa
     2221 agaagttgtg gtggaacgaa ttatcaattc attgaaacca gaaattgaaa aatcattaac
     2281 agacgtgcaa gaaaaaactt tagagattgt cgaaaaaaca aagtcagaaa atgaagctga
     2341 gttccatgat acaaaagaga aaacaacaga tgtggttgtc aaatattttg cgaacaaaga
     2401 agaagttgaa aagcaggtag ctaacaacac gaaagaatta gaaacaaaac ttgtagagca
     2461 acaaaaaact atcgaagaat ataaagatcg aatgaaagag gtcaaagatt tagcagaggg
     2521 ttataaatta aatagcgaaa agttggcaga acaaagtgaa gctttaagag aagacttatc
     2581 tcgttcccga agtgatctag ctcaaatggc agaaaaaacg aaatcattag cagttgctac
     2641 agaaaatcaa tcaagttcaa aacgtgattt tacaagtaaa aagatggcta ctatcatttc
     2701 tgtcgctgca atggcttgcg gaacgatctt gggatcgtcc tactttatta gtcataataa
     2761 tcaacaagca caggctcaaa ctcaaacatc tatttcgaga gtggaaaatt cgattgggac
     2821 aaatacgaac tctgcttcaa caaatagtag ctatgaagca acaccaacta cagagttcaa
     2881 agttgggaaa gatgttgtca tcagtattga tgggaaaaca actaaggctg aagtgatttc
     2941 tattgatgga gaaaaagcag ttgtgaaaac tccagacgga aatcagtaca aaattcaagc
     3001 tcacttatct gataataact aaggagggct attatgcgag aagaaaaaat agtttatcca
     3061 gtaattcaaa acaatcaaga aaacatgacc tcttctcagc acttacagga agcacagctc
     3121 gtgtatcagg taaatgaact accacaaacg aacgagacaa tgagcaagtc aatatcttta
     3181 attggcttgc ttattgttgc aatagtgata actgtaggtg tttaccgtaa gttgaacgtg
     3241 aaaggaagta tttctgatgg cagataactc aaactatgga tttaaacata acttacatca
     3301 aaaaccttct gaaacaattg tcagagttga atggcttgaa aagcatgtca atagcaccta
     3361 ttctgttaca gcaaaaagat taattgtatc agctatagtt gttgcagtcg ttgttcttgg
     3421 atcaattgtt tacttagttt ctgatacgat taaactgaaa tttatttttg ttccaacgtt
     3481 attaatgata attggatttc ttattctaat tagccagtat atgtttattg atccatctag
     3541 tggttcattg atttctggtt ctattagact gttcaaatca gtagttaatt cagcaagagt
     3601 atctatgggg aaaaagaaac catttcgagg cacagagatt ttaaaagtga aacaagatgg
     3661 aacaatcgta tatcgagatg ggagtgtagc attaatgttc aatctagatg gggctacatc
     3721 actaaccgct tttccagaag aagttatggc tcaggaatcg aaatcaatac aatatcaaaa
     3781 taccagagat cgttccttag ctgaaatcaa aatcacttca tctcaacctc agaatacgga
     3841 aagacaaact cgaaatatga aaatacaaaa acgaaatgca aggagtaaca aagcagtctt
     3901 tgacataata gatcagcaag aacgatttgt atcagaatat attgatggtg tgaaaacaac
     3961 agtaatccaa tatcttatct tgaaatctca atctaagatg gtattggatg aaggtgtgga
     4021 acgtttagaa aactatacga gtagaggact ttattatagt gtcactcagt tgtcagctaa
     4081 acaaacaaaa gaaatattat ctgatattta tgcactgaaa tagaggtgag agattaaatg
     4141 gaaattatgg gtagatcaca actattaaat ttcaaaaaaa agagaaaaac taaaaaagaa
     4201 caaaaaacta gaaattcaaa ggtagtgaat aatacctttg ttagtgattt aacatcttta
     4261 gttggttatc aatttacacc atcttacatt aggacaggta agcggtgggg aacaattgtg
     4321 aaattagtga atacttatgg aatgaataga aattatggat ttggttggat ggttaaagct
     4381 attcctgaag tgagagtaga aggggtaaaa gcctatctaa ttgaagcaga taagccagtg
     4441 tcacaaaaag atcaacaaga aataatgcac gagaatgttg ggggatcaac gaatagttat
     4501 gaaaataatt tagaaggaaa aaattcagat gctgaaatta gcaaaactgt tatgcggatt
     4561 atggatttga atacagcatc tttactagac agcatggcaa ataaaattat tgattggaag
     4621 ctagaaatcc tattagtatc cgattctcct aataaaatca gcgaacagct aattaaactc
     4681 caacggttgt atgatgaaaa aatgtatgga ttaaagctta tgtcatatgg aggagatcaa
     4741 cgtgatctgt ttgatagact tttaagtgaa ccaatcggga atgtctatga gtatacgtct
     4801 atgagttcgg attttgcagg aaatgatcat gcaattcgca aaggtttaga tgatgcgaat
     4861 ggatgggcta tcggcactat gtctactagc tacgcaagtg gtgtagcttt tatggcactg
     4921 gatgattctt ttacaagtaa agttttagtg gcatcacaca aaaacgctac aattaaaggc
     4981 tatgatgaaa aaatctcagc atcatcactt tgggggcaat tgattgctaa taatgcaatg
     5041 gcaaatcata aacgaacttt tcatattgtc ttaaataaat ttgattactt tggtgatcca
     5101 actggtagaa catttgttgc caaaccttcg ttaaacagtg taatagaaaa ggttaatttg
     5161 gctgaagaag gtcttaactc tttggagctt tttggtaatc aaaaggacat tggtgattta
     5221 tatagacgga atctcgataa actaactcag attttttatt tgttagcagg cagaacatta
     5281 gattctgcaa ctagagtttt attgaaaaaa gaattaggta acttttatat aagtcagaaa
     5341 ctttgggata ttaattatga agaacgttca aaacttttgg tgaacagtag tgatacaatg
     5401 ccagttcttg gggattttat tagctatttg actaatgctg aaacaaaagc caaaaaatct
     5461 ggtactgaaa aagaagtcga tcgtattatt gtattaaaag acacattgag aacagcatta
     5521 gacagcttct ctaggatgtt taatcaaaga acgacactga aagaaatcgg taaaagtgac
     5581 aaattacagt tttactataa cttaggtgat ctaagagcag aaaccaatgt tatggaagct
     5641 caatttttaa atacctttga tttcatcaca tcacaagcaa aaaaagatga tattgttatg
     5701 attcacggct tcgataagtt atctattgaa accgttgggc taattaaaaa aagattaaca
     5761 atgcttgaag aagatggaat tcgtcttgcc tttctatttg atacaatcgg ttcaggagaa
     5821 gacaatgaat acaaagaaga tattgaaaca gctgatatat tcaatacaga tggaaaaatt
     5881 taccaaagtt ttgaaagtga ttttaattac actatcattg gaaagatgac cagtggagag
     5941 ttaatgaagt atgaaaaaaa agttgctcct gctagaaaag gagaagatga tcaaaaactt
     6001 tctgttgatt tgagacagta tttgacaaca ggaagaagta ataaatttca gatcaggaga
     6061 ccagtggatc tgactaataa ttttgttatt ggagagttta ttctttaagt tttatctttg
     6121 attttgagtg tataaggaaa ggataagaaa aaacatgtat atttgagact aaaaacagtc
     6181 ttagagaaca tggtacgttt tgtgaaaaga gaactataga aatttcataa agtgaaacct
     6241 tactgagtga aataattata gtggaggtta aataatgaaa ccaaacataa aaattttaga
     6301 tcgaatattt ctaggtagag acacagaagt aatcctaatt caacatgaag aaggatttga
     6361 agttagtata ggtattcaga aacttcaaaa acctcattat tgtaatcagt tgtataaaaa
     6421 ctttactgat gaagaaaaag cgagagtttt
//
LOCUS       DAXWOO010000150         6432 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.153, whole genome shotgun sequence.
ACCESSION   DAXWOO010000150 DAXWOO010000000
VERSION     DAXWOO010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.153"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            208..2250
                     /locus_tag="ACGE70_002361"
     CDS             208..2250
                     /locus_tag="ACGE70_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFF8693969.1"
                     /translation="MNKRIREILKLIIKNPEMKLSTLTSELDLTRRQINYAINQFNEE
                     LKTKNIPLIQRSHSGNITVPIEVIQMISQPDYDIGDDQELVVLTDSERQALIVMTLIA
                     NIDYISLDHLVDAVDVSKTTVMEDIKRTEPFIRNYSLTIRYNRMNGYQLVGSEHRILQ
                     LLSDLVKRFPIFRREMIRRKLAQDVSEEEIVHLIHNMEQMLHLSYSDESVDYLQTSAR
                     FLVSRGIKMSDKHAFFPDDVTDTPEFRMLMILVHETGWSLSKSYMEWFTLLFLTSNIF
                     ESKTTQNYDSDQELRKLISQMVENFQNQTLILIDDREDFERRILSHLRPACFRIRYNL
                     SLGVYSIDSLIKDSNHAILIDLMKELIIPIENWLNKAFPNDELDLLSYYFGYQLSNHN
                     KLNKQRQRAVVVCTNGVMVSKLVRANMEKLFPEIHFLASLSVRDFYKFEADYDLVFTT
                     TPLKSSMLQFMIEPIMTYQQQISLRYRVLSDLGITKVDRAMDELLTIIRKYANITNPV
                     SLREELQYFLIKEDGSTPLDNFNVLPSLTYYLRPSYVQIVEEEMSWERAVEVACRPLL
                     EHQIIDSRFIADCLDQIRQPGYAGYLGMKTCIPHTTVDKGVISDGVSILVCKKPIRFP
                     NGEDVSLILPLSFFDLTKHLRAINQIADIAKDDSLIEQLLIADDEKTVYQLLRQRS"
     gene            2261..2881
                     /locus_tag="ACGE70_002362"
     CDS             2261..2881
                     /locus_tag="ACGE70_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFF8693970.1"
                     /translation="MRLADYPKLTVIMRGYTFDQADAILQAMQEFDHQFAVEMTMNTK
                     GALENIHELNQRYGDTTYIGAGTVRTLEQAQACYEAGAKFLLGPHMFTKEMLSYANKK
                     GLLAVPAAMTPSEVDRMFKEGADIVKVFPAAVVTPRFFKDIQAPLGKLPLMGVGGISK
                     ENAKEFFEQGASYLGLGSGMFNKQDIEELNVKNLARSMKELIEAIK"
     gene            2893..3351
                     /locus_tag="ACGE70_002363"
     CDS             2893..3351
                     /locus_tag="ACGE70_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFF8693971.1"
                     /translation="MELYLNEELVFRNVQAKTDSEVLAFLASEMYKKNYVKEEYIQAI
                     QEREKEYPTGLPSTPPGIAIPHANYEMVNKTTLAIATLKDPVLFHNMEDNNEQLPIQI
                     VIMMAIGEPHGQVEMLQKIVGIIQDEPLRQEMIRAGNDTELLELLKKAVF"
     gene            3370..3648
                     /locus_tag="ACGE70_002364"
     CDS             3370..3648
                     /locus_tag="ACGE70_002364"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFF8693972.1"
                     /translation="MKRILVACGNGIATSTVVATKVREYLQQHGIDVSTTQTKLMEVP
                     GKVQDYDLLVTTGQFDGQTGGVPVIKGMPILTGIGADQTMEEILNLLK"
     gene            3673..5040
                     /locus_tag="ACGE70_002365"
     CDS             3673..5040
                     /locus_tag="ACGE70_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002386123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFF8693973.1"
                     /translation="MKIIMNVFNFFIDAGPTVMLPVIITIIGLIFGLKISRAFKSGLT
                     LGIGFAGIKLILDFMTTNVGPAAKAMVDRTGVKLDALDVGWGSIAAVTWASPIIPILI
                     FAILLVNIVLLILKRTHTLDVDIWNYHHMAIVGVIVYFVTKNVFLGVGASVVMAIATF
                     KISDWSQPMVESFFGIPGVSLPTVSALSSLVIAWPLNWLLDRIPLFRKSKFTIKDAQK
                     YLGFFGDSMIMGLVIGMVIGVLAGYDIKAVLQLGVSMSAVLVLIPKMTSLFMEGLMPI
                     SDAAQKWSQKKFKGTRLFIGLDAAVVVGNPDVITTALIIIPLTIALALVLPGNRVLPF
                     ADLAVVPFRVAMVVALTRGNLLKNIIIGLVVTASLLWCGSATAPMLTAIAKSVGINLG
                     TSSMLISSFAATSMIQSYLVFIAFAYKPIIGIPVLLLAFAGAWYYFDGIKKVNHEMDE
                     PLKAQ"
     gene            5055..6200
                     /gene="dgoD"
                     /locus_tag="ACGE70_002366"
     CDS             5055..6200
                     /gene="dgoD"
                     /locus_tag="ACGE70_002366"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HFF8693974.1"
                     /translation="MKISDVTVYKVKPRWIFVKISTDEGIEGWGEMISGTKTETVVAG
                     AYEIGNRLIGRNPFEIERLFQEMHRSFFRGGPINGTIVSGLEMALWDIKGKAFNVPVY
                     ELLGGAARDKIKVYSWIGGDRPSEVAEQAQDRFDRGFTAVKMNATEELHYIDSYQKVD
                     EVVERVASIRERFGDAMDIGVDFHGRVHKPMAKVLAKALEPFHPMFLEEVVLPENEEH
                     FKEVADSVAVPLATGERLYTRWQFKNLFKQGTVDIIQPDVALCGGILETRKIAAMAEA
                     YDMAVAPHAPYGPVALAATLQVDSCTPNVFIQEQSLGIHYNKGFDLLDFVKNKEVFQY
                     KDGYVDLPSKPGLGLEMDEDKIKEIAQEGLIWTNPQWKNYDGTIAEW"
     gene            6227..>6432
                     /locus_tag="ACGE70_002367"
     CDS             6227..>6432
                     /locus_tag="ACGE70_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693975.1"
                     /translation="MAEALMITVFLGLLIILQIIDQKKIIVFAQSSPVRKSLSILLTV
                     AILLIFWPEHLTDQIKLVAFASVI"
BASE COUNT         2002 a         1073 c         1420 g         1937 t
ORIGIN      
        1 catccaatat tctaccgaat atttaatatt cacattagcc ctaaaataat tgtcatacca
       61 tgttacattt gatataacag cgttcatttt aaaaatagct gttagtgtga caaaacatta
      121 aaccaagtgt gacaaaaaac gtgatgaaag ctttccggaa acgtatgtga acaagtatat
      181 aatgattttg tcaggaggat agtcaaaatg aataaacgaa ttcgtgaaat actcaaactg
      241 attattaaaa accctgaaat gaaactttcc actttaacaa gtgaacttga tttgacacgt
      301 cgtcaaatca attacgcgat caatcaattt aatgaagaat tgaaaacaaa gaacattccg
      361 ttaattcaga gaagtcattc tggaaatatt acggtaccaa tcgaggttat ccaaatgata
      421 tcacagcctg attacgacat aggtgatgat caggaacttg tcgttctcac tgatagtgaa
      481 agacaagcat tgatagtgat gactctgatc gctaatatcg attacatcag tctggatcat
      541 ttggtagatg cggttgatgt tagtaagacg actgttatgg aagatatcaa acgaacggaa
      601 ccgttcatca gaaattatag tctgacaatt cgatataatc ggatgaatgg ttaccaattg
      661 gttggttcgg aacaccgtat cttacagctg ttatcggatc tagtaaaacg gtttccgatt
      721 tttcgccgag aaatgattcg cagaaaattg gcacaagatg tttctgaaga agaaatcgtc
      781 catttgatcc ataacatgga acaaatgttg catcttagtt attctgatga gtcggtagac
      841 tatttgcaga catcagcgag atttttagta agccgtggga tcaagatgtc agataagcac
      901 gcgttttttc ccgatgatgt gacggatact cctgaattcc gaatgctgat gatcttggta
      961 catgaaacag gttggagtct atccaaaagt tatatggagt ggttcacgtt attattttta
     1021 acttccaata tttttgagag caaaactact caaaattatg attcggatca agaattgcga
     1081 aaattgatca gccaaatggt tgaaaatttc caaaatcaaa cgttgatttt gatcgatgac
     1141 cgagaagact ttgaacgtag gatactgagt catctgcgtc cagcctgttt tcgaatacgc
     1201 tataacttga gtttaggagt ttattcaata gatagtctga tcaaggatag taatcatgcg
     1261 attttgattg atttgatgaa agaattgatt attcctattg agaattggct aaataaggcc
     1321 tttccaaatg atgaattaga tcttttatcc tattactttg gctatcaatt aagtaatcat
     1381 aataaattga ataagcaacg acaaagggct gtagttgttt gtactaatgg tgtcatggtc
     1441 tcaaagttag tacgagccaa tatggaaaaa ctttttccag agatccattt tttagcttcc
     1501 ctatctgtgc gtgatttcta caaatttgaa gctgattatg atctggtctt tactacgacg
     1561 ccactaaaaa gttctatgct gcagtttatg atcgagccaa tcatgacata tcagcaacaa
     1621 atcagtttac gatatcgagt tttgagtgat ttaggaatta ctaaggttga tcgagcaatg
     1681 gatgaattgt taacaatcat taggaaatat gcaaatatca ccaatccagt ttcgttgcga
     1741 gaagaactgc agtacttttt gatcaaggaa gatggaagca cgccgctgga caacttcaat
     1801 gtattgcctt cattgactta ttacttgcgg ccgagttatg tacaaatcgt tgaagaagag
     1861 atgtcttggg aacgagcagt ggaggtggct tgccgaccgt tattggaaca tcaaatcatt
     1921 gactcgcgtt ttatcgctga ttgcttggat caaatcagac aaccgggata tgcaggttac
     1981 ttgggaatga agacttgcat tccccataca acggtcgata aaggtgtaat cagtgacggt
     2041 gtcagcattt tagtttgtaa aaagccgata cgatttccta atggtgaaga tgtcagcttg
     2101 attttgccgc tttcattttt tgatttaacg aagcatctgc gagccatcaa tcaaatcgct
     2161 gatatcgcca aagatgattc attgattgag cagttattga ttgctgatga cgaaaagaca
     2221 gtttatcaac tattaagaca aagatcttaa ggaggaaaac atgagactag cagattatcc
     2281 taaattgaca gtgatcatgc gtggttatac ttttgatcag gcagatgcta ttttacaagc
     2341 aatgcaggaa tttgaccatc aatttgccgt agagatgaca atgaacacaa aaggagctct
     2401 tgaaaatata catgaactaa atcaacgtta tggtgacacg acttatatcg gcgctgggac
     2461 ggtaaggaca ttagaacaag cccaagcctg ctatgaggct ggtgcaaaat ttttattagg
     2521 accacatatg ttcacaaaag aaatgttgtc ttacgccaat aagaaaggat tattggcggt
     2581 acctgccgca atgactcctt cagaagtcga ccgtatgttt aaagaaggag ctgatattgt
     2641 aaaagtattt cctgcagcgg ttgttactcc acgttttttc aaggatattc aagcaccttt
     2701 aggtaagtta ccactaatgg gggttggtgg aatcagtaag gagaatgcga aagagttttt
     2761 tgaacaaggt gctagctatt taggtcttgg ttccggtatg ttcaacaagc aagacatcga
     2821 agagctaaat gttaaaaact tagcacgttc gatgaaagaa ttaattgagg ctattaaata
     2881 ggagtgagga ctttggaact ttatttgaat gaggaactcg tttttcgaaa tgttcaagca
     2941 aaaacagata gtgaagtctt agcgttttta gcaagcgaga tgtacaaaaa aaactacgtg
     3001 aaggaggaat atattcaagc aatccaagaa cgggaaaaag aataccctac tggattacca
     3061 tcaacaccac caggaatcgc aattccccat gcaaactatg aaatggtcaa taaaacaact
     3121 ttagcaattg cgactcttaa agatccggtc ctatttcata atatggaaga taacaatgag
     3181 cagttaccaa tccaaattgt gatcatgatg gcgattgggg aaccacatgg gcaagttgaa
     3241 atgttacaaa aaattgttgg aatcatacaa gatgagccac tgcggcaaga aatgatcaga
     3301 gcaggcaatg atactgaatt attagaacta ttaaagaaag cggtttttta atataaggag
     3361 gaaatcataa tgaaacgaat tttagtagca tgcggaaatg gtatcgccac ttcaacagta
     3421 gtagcaacaa aagttagaga gtatttgcaa caacatggca ttgatgtttc aacgactcaa
     3481 acaaaattga tggaagttcc aggaaaagtt caagactatg atctattggt gacgactggt
     3541 caatttgatg gtcaaacagg tggtgtacca gtaatcaaag ggatgccgat tttaacaggt
     3601 atcggtgccg atcagacaat ggaagagatt ttgaacttac tgaaataata gctgatcaaa
     3661 ggagctgaaa aaatgaaaat cattatgaac gtctttaact tctttattga tgctggacca
     3721 acagttatgt tgccagtcat catcacgatt attggtttga tttttgggtt aaagatttct
     3781 cgtgctttta aatcaggttt gacattaggt atcggttttg ccgggatcaa gttgatattg
     3841 gactttatga cgaccaatgt tggaccagct gccaaggcga tggttgatcg aactggtgtt
     3901 aaactagatg ctttggatgt tggctggggt tcgattgcgg ctgttacttg ggcatcgcca
     3961 atcattccaa tcttgatttt tgccattcta ttggtcaata tcgttttgct gattttgaaa
     4021 cgtacacata cgctggatgt tgatatctgg aactatcacc atatggcaat cgttggtgtt
     4081 atagtgtatt ttgtgactaa gaatgtcttc ttaggtgtag gtgcatcagt agtgatggca
     4141 attgcgactt ttaagatttc agactggtcg caaccgatgg tagaaagttt cttcggaatc
     4201 cctggtgttt ctttaccaac tgtttctgcg ctttcatcac tggttattgc ttggccgtta
     4261 aattggctgc tggatcgtat cccgttattc agaaaatcaa aattcaccat caaagatgca
     4321 caaaaatatt tgggattttt tggtgattct atgatcatgg ggttagttat tgggatggtg
     4381 attggtgttt tagccggcta tgatatcaaa gcggttttac aattaggtgt cagcatgtca
     4441 gctgttttgg ttttgattcc taagatgact tcattattta tggaaggatt gatgccaatt
     4501 tcagatgctg cacaaaaatg gtcacaaaaa aaattcaaag gtacacgttt atttatcgga
     4561 ttggatgctg cagttgttgt aggtaatcca gatgttatca caacagcatt gatcattatt
     4621 ccactaacga ttgcattagc tttagtattg ccaggaaacc gtgtgttacc attcgcagat
     4681 ttagcggttg ttcctttccg cgtggcaatg gtcgttgcct tgactcgtgg taatttatta
     4741 aaaaatatca ttattggctt agttgtgaca gcttcattgc tatggtgtgg gtcagctact
     4801 gcgccaatgc tgacagcaat tgccaaatcg gttggtatta acttaggaac ttcttcaatg
     4861 ttaatctcat catttgctgc gacttccatg atccaaagtt atttggtatt tatcgcattt
     4921 gcttacaaac caatcatcgg tattccagtg ttgttattag cctttgcggg cgcatggtat
     4981 tacttcgatg gtatcaaaaa agttaatcat gaaatggatg aaccattgaa ggcacaatag
     5041 taggagggtt taaattgaaa atttcagatg tcacagtgta taaagtaaaa ccaagatgga
     5101 tttttgttaa gatctcgact gacgaaggaa tcgaaggctg gggagaaatg atctcaggaa
     5161 ctaaaacaga aacggttgtt gctggagctt atgagatagg aaaccggctg attggacgta
     5221 atccgtttga aatcgagcgc ctcttccaag aaatgcatcg atctttcttc cgaggcggtc
     5281 ctattaatgg aaccatcgtt tcgggtttag aaatggctct gtgggatatc aaaggaaaag
     5341 cgtttaatgt tccggtttat gaattattgg gcggtgcagc acgtgataaa atcaaggttt
     5401 attcttggat tggtggagat cgaccaagtg aagttgcaga acaagcacaa gatcggtttg
     5461 atcggggctt tacggccgtc aagatgaacg cgacagaaga gctgcattat atcgattcct
     5521 accaaaaagt tgatgaagtg gtggaacgag ttgcgtctat tcgcgaacgt tttggtgatg
     5581 caatggatat tggtgttgat tttcatggac gggtccataa accgatggca aaagtgctag
     5641 caaaagcttt ggagccattc catccaatgt ttttggaaga agtcgtttta cctgaaaatg
     5701 aagaacactt taaagaagta gctgattctg tggctgtacc attagctact ggtgaacgct
     5761 tgtacactcg ttggcaattc aagaacctct ttaaacaagg tactgttgat attatccaac
     5821 cagatgtggc tctttgtggg ggaatcttgg aaacccgtaa aatcgcagcg atggcggaag
     5881 cttacgacat ggctgttgcg cctcatgcgc catatggtcc tgttgcacta gcagcaactc
     5941 tgcaagtaga ctcatgtacg ccaaacgtct ttatccaaga acaaagctta gggatccact
     6001 ataacaaagg ttttgatctg ttagattttg tgaagaacaa agaagttttc caatataaag
     6061 acggctacgt agatctacct tcaaaaccag gtctgggctt ggaaatggat gaagacaaaa
     6121 tcaaagaaat tgcacaagaa ggtttgattt ggaccaatcc gcaatggaaa aattatgacg
     6181 gtacgatcgc tgagtggtga taaatgaaaa taaaggatga tgaaagatgg cagaagcact
     6241 tatgatcaca gtttttttag gactgctgat tattttgcaa ataatcgatc aaaagaaaat
     6301 tatcgtgttt gctcagagta gtcctgtaag gaagagtttg agtattttac tgacagtcgc
     6361 catcctgttg attttctggc cggaacatct gactgatcag attaaactgg ttgcttttgc
     6421 ttctgtcatt tt
//
LOCUS       DAXWOO010000151         6321 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.154, whole genome shotgun sequence.
ACCESSION   DAXWOO010000151 DAXWOO010000000
VERSION     DAXWOO010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.154"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(31..633)
                     /gene="nrdG"
                     /locus_tag="ACGE70_002368"
     CDS             complement(31..633)
                     /gene="nrdG"
                     /locus_tag="ACGE70_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFF8693976.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(948..3137)
                     /gene="nrdD"
                     /locus_tag="ACGE70_002369"
     CDS             complement(948..3137)
                     /gene="nrdD"
                     /locus_tag="ACGE70_002369"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFF8693977.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            3463..4230
                     /locus_tag="ACGE70_002370"
     CDS             3463..4230
                     /locus_tag="ACGE70_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8693978.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            4220..6262
                     /locus_tag="ACGE70_002371"
     CDS             4220..6262
                     /locus_tag="ACGE70_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8693979.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT         1873 a         1123 c         1215 g         2110 t
ORIGIN      
        1 ggaaatccgt tttctctccc aaagctgttt ttatatcaag tcttgctttt tgatttgttc
       61 ataggtttct gttgcatccg tacatttttc ccaaatcaca acttgattgg attctaaaga
      121 tttttggacg tcaatgatcc gctggttgct acttccgcga aattgtagtt ttaggtcacg
      181 tttgcttaat tcaaagcgtc cgtcaacaag gatatcgatt tgcgaaagga gttctaattt
      241 atcttctgaa tcttgtagca attcatcaaa ggtatagcct gtccatgacc agatatcttt
      301 tgtcgtaccg aactcttttc ggatacgtcg tacgacttgc agacaaacat ctgtattcaa
      361 aaaaggttcg ccaccaagta aagttaatcc ttgcacgtaa tcgttgcgca agtcagcgat
      421 gatttgttct tctaattcag gtgtgaatgg tttgccatat cggaaatttt gtgcagcttt
      481 gttgaagcag ccttcgcagg caaataagca gccactaacg taaagactgt tgcggacacc
      541 ttcaccatcg acaaaattaa acggtttgta gtcagcaata tagttttggc tgtagtcttt
      601 tgctaaccat tcttttggtt ttggatttct cataaaaaaa cttctttcat ttaaggttca
      661 aagatatagc tattgaagat aaaatctact cgaccacata aataagaaca aactgaccta
      721 aatccattgt tcaagaaagc ttgttcttat tttgatagag ggcgttcaaa gatacaaagc
      781 catcgaaaga gggtggtgaa aaaagacttg tcatagcctc ttattctgaa taaacggtgt
      841 tcaaaagctt gcatctgcag taataagccc aactaaacga aaaatatgag gaactatttt
      901 tgtttagcta ttcttattac ttgatgcagg acagcttttt cacaacctca tttcatgtgt
      961 ttcacacgtg aggaaatttc tttatggcgg ccatgtacca tcggacgagc ttgcggattt
     1021 cctaaatagc cacaagtacg tttgactaca tcacatgttt ttggatcatg atttccacat
     1081 tctggacatt cgaaccctcg ttctgtcggt ttgaaatcac cttcgaaacc acattgatag
     1141 caatgatcga ttggcgtatt cgtaccaaga tacccgattt tgtcatatgc aaaatcccat
     1201 acagcttcta aagctttcgg attttgttgg agcatagggt attcacagta gtggatgaat
     1261 ccacctgaac aatattttgg ataatccttc tcaaattcca atttttcaaa tggcgttggg
     1321 tttttacgca catcatagtg gaaactgttt gtataatatt ctttatctgt gatattttcg
     1381 atgatgccga atttttctgt atccaaacgg cagaaacggt cagttagact ttcacttgga
     1441 gttgaataga cactgaaatg ataaccatat tcatcccccc atgcatctgc attagctttt
     1501 aattctttga cgatatcaag tgtgaaagct tttgcttctg ggtttgtttc ccattcgcca
     1561 ccgaagaaag ctgaagcaac ttcatacaat ccaatgtagc ctaacgatac agtagctcga
     1621 cggtttttga ataattcatt gacagaatcg gtacgtgaaa gacgtttccc gaaagcacca
     1681 tacatatata aaattggcgc atttgctgga atggcttctt tacaacgttc tacacgatag
     1741 agaagcgcat ctttcatgat ttctaagcgg tcagacagta actgccagaa tttattctga
     1801 tctccttttg attcgatcgc gattcttggc aaattcaatg taacaacacc taaattcatt
     1861 cgtccgacat ttacttcttg accattttcg tctttccacc cttgcaagaa tgaacgacag
     1921 cccataggta ctttgaaact tccagttaat tcaacgattt tatcgtaatt cagaatatca
     1981 ggatacattc ttttcgttgc acattccaat gctagttgtt tgatgtcgta attaggatct
     2041 tcagggttta agttcacacc ttttttcaaa gtgaagatca gtttagggaa gatagctgtc
     2101 cgcttttcac tccctaatcc attgattcga atctgtaaaa ttgctttttg aatttctctt
     2161 tcaaaccagt tttctcctag tccaaatcct aatgaagtga aaggagtttg tccatttgaa
     2221 gtaaataagg tattgatttc atattccaaa ctttgcatag catcgaagat atcttttttg
     2281 gtttttgctt ttgcatattc tttttggcgt tctggcgaat cgatccattc ttccgcgtct
     2341 tttagatgtt ttttatagtt caattctgca aaaggagcaa gcaattcatc tgttcggtcg
     2401 gcagaacagc caccatattg acttgacgct acattggcaa taatctgtga gatttgtgct
     2461 gtggcggtct ggatagattt tggcgattcg acttcggcat tgccgatttt gaacccattg
     2521 ttcagcatgc ctttgaaatc aatcaagcag caattcgtca tcggtgtata aggatgataa
     2581 tccaaatcat ggtaatggat atcgcctttt tgatgcgcat ttgctatatg tggtggcaac
     2641 attttcagtc cgattgattt tccaacgatc ccggctgtca gatctcgctg tgtattgaaa
     2701 acattactgt ctttatttgc gttttcgttc acgacagttt ggtccttgtt gattagcttt
     2761 tcaatcgaaa agttgatatc tgttgcttta ctgcgagcaa aatctcgttt ggtacgataa
     2821 ttgatgtact cttctgctaa ttcatattcg tttttttcta gtaatatatg ttccacgatg
     2881 ttttgaatct catagatttt gacattttca gcaaaccggc ttttgatttc ttggacaacc
     2941 aagtctgcaa tcatttgaat tttttcataa gtaagtggtg ataatgagcc gtgaattttc
     3001 ttttctgctt tgatcagtgc ttcattgatc ttttgttcat cgaaatcaac atggcggcca
     3061 tcgcgtttga tgactttcat ctgggatgtc tctggctgct gagtaacgac agttttttct
     3121 tcataattta ctttcattat cttcaactcc tgctaaattg ttcttgtcta tcataaaaca
     3181 aaatcgggga aggagcaact aaatatcggc tatatatagt attcaatgtg ctgtataatt
     3241 ggatttctat actatatgta gtgtatattt tattgtgaaa aaaggtgaaa acttgtagct
     3301 ggtggatttg ttttttgcat atttaatttt gctgaataaa ataattgaat aaaatttttt
     3361 attcagtcca agtaaaagtg acaaaaacat aagagcaaat tattcgtaga tatgctacta
     3421 tgcaaataaa tagaaatgaa aaatgaaaga ggaggagaag aattggaaaa gatgatcgaa
     3481 gtgaaacatc taagtaaaag ctacggcagc aaactttctc ccgtcaaagc cttagataat
     3541 atctcattca cagtagaaga aggcgagttt gttgggatta tgggcccaag tggtgcagga
     3601 aagacaacgc tgatgaatat cttgtccacg atcaatttgc ctacgatggg cagtgtctcg
     3661 atccaaggaa aagaaatcac aaaaatgaaa aacagtgagc tcagtgactt tcgtcgaaaa
     3721 aaactaggat tcatttttca agagttcaat ttgatcgata ccctaaatgc aaaagacaat
     3781 atcctattgc ccctagcagt tgaacggatg actaaagagg aaatggaaaa gagagtaaga
     3841 catgtagctc aattgttgaa tatagaagaa ctgctcaaac ggtatcccga tgaactttct
     3901 gtcggacaga gacaacgaat tgcagctgct agagcacttg ttgttcagcc acaagtgatt
     3961 tttgcagatg aaccaactgg ctctttggac tcgaagtcgg ctacagaatt gttgaactac
     4021 ttgaagacca tgaatcaaaa agaaaaagca acgatactgt tagtcaccca tgatccatat
     4081 acagcaagct actgcgatag aattttattt attaaagatg gtgtgatttt ttctgaagtt
     4141 gtcagaagag ggacaagaag agagttcttt gagaaagtaa tagacatgca ggcgacgatt
     4201 ggcggaggag gaaaaatgaa tgctgtataa actagccgtt aaaagttttt ttcggcagtc
     4261 acgaggctat cttgtttatt ttttcagttt gactttatcc gtaatgatct attattcctt
     4321 cagcgcaatg acctacgatc aatctttagt cagaagagca agtcaagata cttcgataga
     4381 tggtggattg agttttggtg gatttatcat tactgttgtt ttgctttttt tcgtattcag
     4441 tgccaaccga tttttcttga atagaaggca aaaggaaata ggcatctatc agctttttgg
     4501 tatgaataaa ctgcagatat caggtatata tgtgttagaa atcatgataa ttggattgtt
     4561 cgcctgtatt tctgggatat tactcggtat tattttttcc aaactctttt caatgatttt
     4621 ggttcgaatg atggatatgg atcttactag tccatttttt atctccattc catcagtagt
     4681 agatacttta tttgcttttt tcatcatcct tttagcagta tctgtatatt caatatggaa
     4741 aatatggcgc tatccaatga ttcgaagttt tggagaaaaa gaacaagcag atagttcaac
     4801 aatgcgcttc aggacaaggc accgattact cgcagtcatc gggttactac ttttaacgag
     4861 cggatatttt ggagctagtc attttaggga aatcacttca tggatgatca gttatcatta
     4921 tcttgataca ttagttttct tttttccttt tttaattttt ttcgtttgtg tggtcggtac
     4981 ttatttgttt ttctgttata catttcggtt atttttgatc atattgagta aatggaagat
     5041 ttattactat ggtatcaatt ttttgataat gggtaacacg caagttcatt tgctcaaaag
     5101 ttggcgtaca aattctctga ttacagtcat tctcggaatt tctttaatga caattggcgg
     5161 cacggctagt atctctacta ttttatccta ccaggagaaa gtttcctccc cgatggacta
     5221 tcagtttgat gttgagacag agaaaaaaat caagccgatt ctagcagagg aagggcagaa
     5281 gataactaaa gaaatcacct tgcattataa agtcattggg agtatctata gtcaaaatct
     5341 cgaagaacct atgtttcaaa ctgctaatct gatcacggaa acacaatatc aagccttcag
     5401 aaaagttaat ccagatttgc cgaacatttc tttgaaatca cctggtcata ctgttttgct
     5461 tgatcaaatg caaactcttt ttcggaatat ttcattatat ggatctagta ttggactgcc
     5521 agataatcaa gaattgaaga ttcaacaaat gcttccaagt gtattaggtg acgaaacaat
     5581 ggtatatcta agtcctgtac tgattgtaga tgataccgtt tttgaaaaag caaaaggtgt
     5641 ggattatcaa gtagtcaatg tagatgtatc aggtggaaat aatgaaaaaa tagatgaacg
     5701 actgagcaat gacctgtcga taaagtggct caatgctgtt tattattctt atgatatcgt
     5761 aaatggaagt ctacaaggag aaattagcac aaagaaactt ccggatgacc aaatgaagga
     5821 agcacaattt agccaagaaa gctctcgttt gaatttctct tccagctatt cgaaattgcg
     5881 tgcagttaat cggcgaatcg gtatcaatat ttttgtcaca ctatttgtcg ggatgatcgt
     5941 gattattgct actggtagta ttttgacagt ccggcaatta gcagaagcag aggaagaaaa
     6001 agaaaattat cacctattga ctaagctggg gattccacaa agaaggatca gacggatgat
     6061 cttcgagcaa aacgcactta ccttctttcc acccatgata ttagggatca ctcacgcagt
     6121 atttgcaatc aatgtcttca ctcagtatat aaaaactgcc gattactggt tggcttatct
     6181 tgtgagtggc ttattgatcc ttatctatct tttgctttat gtcattacga gtcagttata
     6241 ttgtcggatt atcgaggaat gatatacgga ataagacaag ataataaata gaactaaatt
     6301 ttgaatacca tttatttgga a
//
LOCUS       DAXWOO010000152         6303 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.155, whole genome shotgun sequence.
ACCESSION   DAXWOO010000152 DAXWOO010000000
VERSION     DAXWOO010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.155"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            334..714
                     /locus_tag="ACGE70_002372"
     CDS             334..714
                     /locus_tag="ACGE70_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFF8693980.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPD
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            696..1610
                     /locus_tag="ACGE70_002373"
     CDS             696..1610
                     /locus_tag="ACGE70_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFF8693981.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     TLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKK
                     KVRSNKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTP
                     EQSEIRPTRRNHEQHVERGTDHQQELSL"
     gene            1570..1848
                     /locus_tag="ACGE70_002374"
     CDS             1570..1848
                     /locus_tag="ACGE70_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693982.1"
                     /translation="MNEEQTTNKNYRSKTLKDIENEIKRQRAFLAPYTQERIQTTMDS
                     QVKKLHQALLDQRNQEQKKALIQRSMDILAPLCCLVLTLLAFWLVWGE"
     gene            1855..2544
                     /locus_tag="ACGE70_002375"
     CDS             1855..2544
                     /locus_tag="ACGE70_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693983.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLV
                     GVIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTA
                     CYEDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQT
                     EEACCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLES
                     VKEWLDQQQQN"
     gene            complement(2665..2751)
                     /locus_tag="ACGE70_002376"
     CDS             complement(2665..2751)
                     /locus_tag="ACGE70_002376"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFF8693984.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
     gene            3427..4377
                     /locus_tag="ACGE70_002377"
     CDS             3427..4377
                     /locus_tag="ACGE70_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFF8693985.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVV
                     GYQFTFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELE
                     LLQQIKQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
     gene            4398..4931
                     /locus_tag="ACGE70_002378"
     CDS             4398..4931
                     /locus_tag="ACGE70_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFF8693986.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLRE
                     QLALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATE
                     TIDKQIDTEKKWWQLWK"
     gene            4947..5117
                     /locus_tag="ACGE70_002379"
     CDS             4947..5117
                     /locus_tag="ACGE70_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693987.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
     gene            5567..6100
                     /locus_tag="ACGE70_002380"
     CDS             5567..6100
                     /locus_tag="ACGE70_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693988.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV
                     VIEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAIN
                     AAFWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQ
                     RGYDAAGRVVATRYSTL"
     gene            6112..>6303
                     /locus_tag="ACGE70_002381"
     CDS             6112..>6303
                     /locus_tag="ACGE70_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8693989.1"
                     /translation="MFLISKSLKSLKTFTKKNPQIATIRIFIFAIAVAIIVNMLSGGQ
                     LYKSGLFLVLILVVLDIIKS"
BASE COUNT         2324 a         1098 c         1175 g         1706 t
ORIGIN      
        1 aaaaaaaaat aaatcgatac attttagaag aatagggttg tttgaatatt acaaagcaac
       61 aatttcacct tcggtgaaat aagcgaaact ttacaaaaag tggctagcaa atgctagcct
      121 ttttttgtat tgtggagcgt ggattttgag ggggattcaa gggggagctt gcgctccttt
      181 gacaagcggt tatttgcacc aaatacggag gtatttggct agcaaatata gagcttgcca
      241 aaccctttta gtgagcttta ctcattgtac ctttttaggt acaatggtat tgcgaacgat
      301 acgtccgtag gacgtactga aagggtggga cttatgagcg aacaacatca aaatttggct
      361 agcgatcatt ctaaaaaata tacctatcga tccgagccaa aacaaatcag ttttcgagtg
      421 agcgaatccg aatttgcaaa gctgaagcag tcagctgaag ctttgcaaat gagtgtgccg
      481 gctttcgtga aagccaaggc acaaaacgcg cgtctggtga caccgaaagt ggcgccagac
      541 attgcccagg caatcgcgcg cgatttagcc aaagctggag ggaatatcaa tcaaattgcc
      601 aagtggtgca atacacacca acacgacgtt gctcctgatg atgctcaacg tttatccgaa
      661 aacctaaaaa tcatgcaaaa ggagctacaa aagatatggc aacagttaaa gtaagtcgaa
      721 ccacttcatg tagccgagca atcaattacg cagaaccacg agcaaccgtc aaaacaggaa
      781 taaactgtga cattgattat gccaaaagtg aaatgaaaca aatacgcacg ctttatggaa
      841 aagacgatcg tgtacaagca cacttattga ttcagtcctt tcgaccagga gaaattaccg
      901 ctgaaaaagc caatcaacta ggggcagagt atgcgaaaaa aatagcccct aaccaccaaa
      961 tcgcgattta cacacataca gacaaagacc atattcataa tcacattgtc attaactccg
     1021 ttaatcttga aactgggaaa aaatttcaag cccatgggca agcattttta aacaaatgtt
     1081 atgacctaaa tgacgagatt tgtcttgaac atgacttaac aatcacggaa cgaggaaaga
     1141 aaaccgaaaa acgaaccatg tctgaaatta aattaaaaga aaaaaatgaa ccggtttgga
     1201 aagacgaaat tcggtttgcg attgatcaaa caatgaaaaa caaaaaaact cgaacgtata
     1261 cccaattttg tgattcactt aaaacattcg gcattcattg cttgaatcgc ggaaaaaatt
     1321 ttacttacga attggtccac aaaaagaaaa aagtacgatc caacaaacta ggaaaggatt
     1381 acgaaaagga gacgatttta catgagctgg acagacgaga acgaacctac aatcaacaac
     1441 gaaccaacga gctacgaaga gctcgagcta gcattcgcga aactgcagaa gaagtacgaa
     1501 gagaacaacg aacttttacg cctgaacaat cggaaattag accgactcga agaaaccatg
     1561 aacaacacgt tgaacgagga acagaccacc aacaagaatt atcgctctaa aacgttaaaa
     1621 gacatcgaaa acgaaataaa gagacaacgt gcctttcttg cgccttatac ccaagaaagg
     1681 atacaaacta cgatggattc acaagtcaaa aaactccacc aagcgctatt agaccagcga
     1741 aaccaagaac aaaaaaaggc tttaattcaa cgatcgatgg atattcttgc ccccttatgt
     1801 tgcttagtac taacattatt agccttttgg ttggtatggg gagaataaac agacatggca
     1861 ttctatttac cctatctact catttctgcc agtattttag gaattatttg gcttagctat
     1921 aaggcttatc aaacaagata ttcattaaaa ggctctaaaa ttcgatttaa gcgattttta
     1981 ttggtcgggg ttatatttgc cttatcaatc gttttaagtg gtttattcgg cgttttagag
     2041 ggtaataaac gtgtttcaag aagcatttta ttaggggata tcactcaaaa gtatgaagca
     2101 gcatgtgata aaaagaaaaa agagcaagca ttagcacaaa aaatggagga atttaccgct
     2161 tgttatgagg acatgaacaa tatttttgtg gatcaagaga aacgtttaac agataaaaac
     2221 atgaaaaaac tcactcgttt ttatcaaaac ttaccagaaa aacagcaaaa agaggtacaa
     2281 gaaaaatatg agcaagtaaa aaaagacgtg caatatttta aagatacgca gacagaagaa
     2341 gcttgttgta acttatttag cgatacaatc cctttttcaa ccagtgaaca agaacgaaaa
     2401 gaacggcaac aatcggtcac atacgaacgt tatgaaacac tgcttcagca agcaacaaac
     2461 atacaaaatc caacgaaaaa agaaacggca ttaaattatc tagaaagtgt caaggaatgg
     2521 ttggatcagc aacaacaaaa ttgaatttga ttagcaatca tgttaaactg aacctgcaaa
     2581 tgatcaaaca ttagatacac gaaagcccca gagaggtcgg attctctggg gctttttttc
     2641 aggttgatgt cgcctttatt gattttactt cttgtttcgg ttattcaacc aataactgaa
     2701 taaagagaca caaacttcta caagaagggg gcaaatgaac cgttcaaaca ttagatacac
     2761 ctccttccag tcgcaagact gtcggatagg cgacaagtta ttatacctta caaaataaaa
     2821 tctgtgtggt gtttttaagt atcccttatt ttcgggttgt tagggcttgc cctgaccgcc
     2881 tgtaaggcgc tttaagaata agataaggtt ttttacccaa gactgtctta aaacgactta
     2941 gacgagctat cagaggcttc ccccacctcc gttactgact tgtttttagc aaaggagcct
     3001 ttaggcgact gcgcagtaca atgtgagagc atagcgatca cactttttta gcgaccgtag
     3061 ggagcaaggt tttgaattgg cagttttgaa aaatgcgcga acctgttggt agaaaaaaga
     3121 agtggttttt aggcgggaaa ttttgtcacc ctcttttata atctcttttt aaaccttttt
     3181 ttaaacctct ttaccgtcgg ctgtgtcctt actccttcaa tggatacagc gttttataaa
     3241 ctacattttg tctgctttta aactacattt tgtctgcttt taaactacat tttgtctgct
     3301 tttaaactac attttgtctg cttttaaact ctttttttgc actcacgtat gttataatat
     3361 gcattatcac agacaaaacg ttgtttataa cgtctgtatc cattgaagga gtaaggagga
     3421 atatctttgg caaatgaaat cgtgaaatac catcatgaat taaataccat tccgttgcgg
     3481 aaatttacac cagttgaaat gaatctattt ttctctattg tgtctagaat gcgcgaaaaa
     3541 ggcgataaaa cagtccgtct tacttttgac caactaaaag atttaagtaa ttataaagca
     3601 actgctaata atcgtttcat taatgattta gaaatgactt atgataaatt aatggattta
     3661 cgttttggta aaagaagtgc taatgggctc gatatagata gattcgttat gtttagtgag
     3721 tttaaaatac gtggaactgc taaagatcca tatgtagaca tacaagttta cgaaaaagct
     3781 ttacctttac taaacaattt agatgagtgg gttcgctatt cactccaaca atttaacgaa
     3841 ttacaaagta gctatgcaaa gactatgttt cgattactaa aacaatatcg aactaaaggt
     3901 tttgcttatt tttctaaaga ggattttcat gaactattag atattccaaa aagttactgg
     3961 aacaaccaag caaacgttga taatcgtgtt ataaagccta tacgacagga attaaccgca
     4021 atcttcaaag gattatcaat caaaaagaaa tacggaaaag ggcgaggaaa acctgtcgtt
     4081 ggctatcaat ttacgtttaa accagaaatc aaaaaagcag atgattttaa taaacatgca
     4141 ccaaccaaag aagaacaaaa taaagcattg gctttagctg ataaacaaaa agaatacgaa
     4201 gaacctgcta caacaattaa cgaaaaaatc aaacagaaga aacgagaact tgaactcctc
     4261 caacaaatca aacaactaga aaaagagaaa gaagctcttg aaaaacaaac agagtccgca
     4321 ctttcgaaaa aagagaaaaa aagtttatta tctgatttag ataatttatt ccagtagcaa
     4381 cctaggaggc aaccagtatg caaccacaag aaacctttac aataaaggaa ttgtcagaac
     4441 tttttaagat gagtaggcaa gcaatatcga aacatatcca aaaattagat tcttccatga
     4501 tagctaaaaa tgagcgtggt tataaggttg tattgcgttc tggagtattg caacttgctc
     4561 gcaacttagg caaccaaatg ttgctagata ggttgcacga gtacgaacaa gacaacgata
     4621 aaaatgttaa tataccaagc aaaaacgtgg ttacaaacaa gttgctagat cagttgcgtg
     4681 aagaaaatga atttttacga gaacaacttg ccctcagaga gcaaaataat tctcatatac
     4741 aaaaactact cgaccagcaa caacaactca ccctacaagc caacaaacaa atcgaaaaac
     4801 tgcaagaaaa actacagttg acttatacag aagaagctcc agagaatgac tcaacgactc
     4861 tttctgaagc aacagaaaca atagacaaac aaattgatac tgaaaaaaaa tggtggcaac
     4921 tttggaaata actggaggga acattcatga caagaaaaaa tcttctattc ggttctattt
     4981 tactaggaat tctaggattc agtccgatgt tattgtcagt gatcaataaa gggaataaac
     5041 atggtgaatt gaaattgaat ccaaattatt acaacagtcc taacgaagaa ttgtttgtaa
     5101 aagaaacaga taaataataa aaaagactgc tctttcgagc agccagttaa aaaataatga
     5161 gtgtttattc ttgaaaaagc aacgcgctta tgaagcgcta ccaaaaattt atcatatttt
     5221 attaaaaata acaacccagt aaaaaatgac tggtaaaaat tcacaaagaa acggaaagaa
     5281 gcaaccgcag aaaatcaagc ttaaaaagag cttttgacct agaccaatcg aaaatcacaa
     5341 agtgagcctc tgtatcggtt gatacaaggg ctacttggca tagtgtacat tatgttaaca
     5401 agaatttaat ataacatatt ttcctaatgc attctaacta taccacatta tcaaaaatat
     5461 aaattatctt tactaaataa aatattgaca agcctatacc ttttgtttta tgattttcac
     5521 aaagaaaacg ttttctatat atttttctgt atcaggagga aattctatga aaaaatttac
     5581 tttaggggta gctttgttaa ctattggggc agttgtttta ccgtcaacta ctatagttta
     5641 tgcttcagaa aattcccaag tggtatcatt aaacgaaaac aatgctactg aaaatgtagt
     5701 tattgagtac actgaagatg gtatggtcgt aactaaatat gaggatggtt atagtgcgga
     5761 agattttgca aattcttcag atatcatgac aagagctgca gttgcaagat ggggcaaaaa
     5821 ggaatataca aatattgctg taaccactgg agtagctgct aatgctatta atgctgcctt
     5881 ttgggcagga gggaaagcta gcacttctat gtttggtatc ccaggttggg caattggtgc
     5941 tttgttaaca ggtgcaagtt ggactaaaaa aggtgcttct ccagggaatg cagttgcaaa
     6001 agaatgggac aaaaatggta atggctggat tggattttat taccaaaggg gatatgatgc
     6061 agctggaaga gttgtagcta ctagatattc aacgttataa gttaaggagt gatgtttttg
     6121 atatctaaat cattaaaatc attaaaaaca tttactaaaa agaatccgca aatagctact
     6181 attcgtattt ttatatttgc tatagctgtt gctataattg ttaatatgct aagcggaggg
     6241 caattataca aatctggttt atttttagtg ttaatcttag ttgtcttaga cataataaag
     6301 tct
//
LOCUS       DAXWOO010000153         6260 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.156, whole genome shotgun sequence.
ACCESSION   DAXWOO010000153 DAXWOO010000000
VERSION     DAXWOO010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.156"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            352..1041
                     /locus_tag="ACGE70_002382"
     CDS             352..1041
                     /locus_tag="ACGE70_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFF8693990.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1028..2236
                     /locus_tag="ACGE70_002383"
     CDS             1028..2236
                     /locus_tag="ACGE70_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFF8693991.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2530..3801
                     /gene="serS"
                     /locus_tag="ACGE70_002384"
     CDS             2530..3801
                     /gene="serS"
                     /locus_tag="ACGE70_002384"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFF8693992.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="ACGE70_002385"
     CDS             complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="ACGE70_002385"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFF8693993.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
BASE COUNT         1922 a         1177 c         1216 g         1945 t
ORIGIN      
        1 tttccaacat gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa
       61 caaataataa tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac
      121 atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc
      181 cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac
      241 caactagatg tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct
      301 gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt
      361 ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag
      421 gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc
      481 cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta
      541 gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact
      601 gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt
      661 aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt
      721 gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat
      781 gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc
      841 tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt
      901 tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga
      961 gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc
     1021 tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac
     1081 ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct
     1141 attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac
     1201 gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg
     1261 gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg
     1321 gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta
     1381 catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg
     1441 agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg
     1501 taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc
     1561 actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc actcagaaga
     1621 agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt
     1681 ggacgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc
     1741 ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa
     1801 aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac
     1861 cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg
     1921 agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa
     1981 aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg
     2041 tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca
     2101 aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc
     2161 tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt
     2221 tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac
     2281 acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa
     2341 ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca
     2401 acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt
     2461 cagactgatt cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga
     2521 ggaaatcata tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa
     2581 ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat
     2641 cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct
     2701 gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa
     2761 gaagtcggtg gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg
     2821 aaacaaattg caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat
     2881 gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca
     2941 aaaccacact gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa
     3001 gtagccggta gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc
     3061 tacaacttca tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct
     3121 tatatcgtca acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat
     3181 gttttccaat tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact
     3241 aactattata atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta
     3301 agccctgctt ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt
     3361 cttcaccaat tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa
     3421 gaattggaaa aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat
     3481 cgtgtgatgg ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta
     3541 gaagtatgga tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa
     3601 gatttccaag cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat
     3661 actcatactc tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa
     3721 aactaccaaa atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg
     3781 aatctgaaag ttattaaata aagaggctgt gaaaaagccg cttagtttcg agtattaaga
     3841 aaaattttgt gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac
     3901 tggcttttga acaccattta tcgagaggtt gtgaaatcag cgaattaacc tgagtattag
     3961 agaaaaaaat ggaaaaatag tccctcatat tttaccgttt gttgaactaa taccgagggt
     4021 cagctgattg aacaccgttt agtggagagg tcgtaagcct accccccagc tcctagttac
     4081 aaggacaaat tcaaaaaaca gctcttcata ttttatgaat gttgtacttg tgattcggag
     4141 gtggcaaggg aacactgttt attcaaaaag aggctgggac agaagcgttt aactccaaga
     4201 aataagaaga aattcacgaa aattgctttt caaatttttg tgaatttctt cttatttccg
     4261 aaggagttgc ttctacttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct
     4321 ttacttttgt cccacgctct ttaatgtttt taagattcaa ctgaatagtt tggtgcttct
     4381 tttgtgattt gtacatcatg agggtgtgat tcttttaatc cactgccgct catttggata
     4441 aattgcgctt catcacgtaa ttgctgtaga ttggctgccc caacataacc cattcctgaa
     4501 cgcaaaccgc cgatcatttg gaaaataata tctgcaacac ttcctttata agcaacccga
     4561 ccttcgattc cttctggtac taatttattt gcttcattga cactaccttg gaagtaacgg
     4621 tcttttgaac ctttttccat cgcgcctaat gagcccatac ctcggtatgt tttgaatcga
     4681 cgtccttgga agatttcgaa ttcacctggt gattcatctg tccctgccaa catacttcct
     4741 agcatcactg catgtccgcc ggcagctaat gctttgacga tgtcaccaga atatttgatc
     4801 cctccgtctg caataatcgc tttgccatat tgacgagcaa ctgatgctgc atcataaata
     4861 gctgtgagct gaggtacacc tacaccggct accacacgtg tcgtacaaat cgatcctggt
     4921 ccgataccaa cttttacgac atctacacct gcatcatata aagcttttgt cgcttcagcc
     4981 gtcgcaacgt ttcctgcaat caatgttgca tctgcaaaag ttgaacggat ttcttgaatt
     5041 tttcggatca cacctgcact atgtccatga gctgtatcga tgatgattgc atctgcccct
     5101 gcttctaata aagcattcgc acgctcaaat gtatcgcttg ttacaccaac ggctgctgct
     5161 acaagcaatc gaccatgttc atcttttgct gcatttggaa attcgatcac tttctcgatg
     5221 tctttgatcg tgatcaatcc gcttaaacga ccttcgttat ctacgattgg taatttttcg
     5281 attttatgtt tttgcaaaat cttttctgca tcttttagag aggtacctac tggggcagtc
     5341 actaagttgt cttttgtcat cacatcagca atcggcatgt gataatctgt tacaaaacgc
     5401 atgtcgcggt ttgtaattat ccctaccaat tttctgtttt caagtgtttc aacgattggc
     5461 acaccactga tacgatattt gctcatcaat tcttctgcgt ctgctacaag atgctgtggc
     5521 gttaagaaga acggatcaat gattacgcca ctttcagaac gtttgacttt tcttacttca
     5581 tccgcttgct ggctgatcgt catgttttta tggatcacac ctagtccgcc ttgtcttgcc
     5641 atagcaatgg ccatcttgct gtcggtaaca gtatccatac ttgcactgat gattggaata
     5701 ttcaacttaa tatttttagc taattgtacg ctcatatcca cttcgtttgg taacacgtga
     5761 ctttctgctg gtatcaataa gacatcatca aaagtaaatc cttttttcgt gaactttgtt
     5821 tcccagttgg acatttttag taccactcct ctaatttagt ttatctatta aaataattga
     5881 ttttttcatt taagtcaatg atctacttgg aatcaagtta gctttctcat agaataccaa
     5941 tataaagcgt ttacatctgt gtatattaaa agagattgta aaaaagctgt cctgcatcaa
     6001 gtaataagaa cagccaatca aaaacagctt ctcatgtttt tgaattggtt gggtttatta
     6061 ccgcagatgc aaacttttga acaccgttta gtggagaggc tgtgaaaaag ctgcttagtt
     6121 tcgagtagta agaaaaattt tgtgaaaatg gctttccaca tttttgcata aattggactt
     6181 aataccgaga aactggcttt tgaacaccgt ttatcgtaaa aagggactgt gacaagactt
     6241 ttgtcacagt cccttactat
//
LOCUS       DAXWOO010000154         6247 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.157, whole genome shotgun sequence.
ACCESSION   DAXWOO010000154 DAXWOO010000000
VERSION     DAXWOO010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.157"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            342..2288
                     /locus_tag="ACGE70_002386"
     CDS             342..2288
                     /locus_tag="ACGE70_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFF8693994.1"
                     /translation="MIERHVNLLKLLSRQEDFKPASFFSDKLAVSTKTIYQDIELLED
                     YVVNYQVTIERLPSLGIKLIGDRKNVEHLISSVENYLEKDIYSPEKRRVEIIKEVLFG
                     DKPVSLTHLSQIFMVSKTSLYNDLRIINKIIGSGEVKLQSSAEGISFTGNEQAIQRAI
                     KQLVFYYANDMESKTFFQVLNMLFDSKVISVIHDLLFNDYSELTEKVSDYYVRSLIST
                     LVIQCNRLLKGHHLTVEEEFLFNSIRYMETYIVANGLIEELKAKLNLPFYDADKEYLC
                     RQLFAHRVTNNLKTSNEEYAMSVHKLIERMSEIEKIDLTQNDRLYQSLLYHVPAMVLR
                     LKKGIKIQNPLLENIKSQYSELFSIVWYAMSILEEEFDVTLTDDEVSLILIHFQIAIE
                     DQSKANNIIIICQYGISSAQLIYNKVRRFLPAKDNVEISTLDKFHLAEIGEVDLIISS
                     LSFDAGGIPLVRVSPLVSKTDYMNIMEAYTKYLIEEPSRFIQNELIPKAVKVPTISKF
                     VQSSLVKTGVQVESKEECLDLMISELEANSLVTEEFRASIFSREKMGNTNLESGVALP
                     HANPSMVIESNIFIVALKNAINWGDRKVKLVIMVSLAEEDVSEIRGVYEELYQFIGQK
                     EMVDRLLQVHDSEKLLRIFQQKGE"
     gene            2291..2746
                     /locus_tag="ACGE70_002387"
     CDS             2291..2746
                     /locus_tag="ACGE70_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFF8693995.1"
                     /translation="MYLDKELCLFQLEVKDQKELFQVMSEQLKNAGCVKDSFLEGITN
                     REQEFPTGLEVNQIGFAIPHTDSAHVNSSQICFASLKEPLVFSDMTDKSHEIPVRLVF
                     MLAMSQPHEQIDTLQNLVSLFQNEEKVNELLACTTKEAFIELVHSAGVY"
     gene            2760..4088
                     /locus_tag="ACGE70_002388"
     CDS             2760..4088
                     /locus_tag="ACGE70_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFF8693996.1"
                     /translation="MDIVLNAFEWFIGLGSNVFLPLIIFVIGILFGLKPGKAFVSGVT
                     VGIGSIGLGLVLSLLSDGLGTAIQQMGEQYGGGLNILDIGVGVGGPLAYSTSLGILMI
                     PISLILNFIFVVLGWTKTLNVDIWNFWFAIFLGMITQSVTGNFWFGLIGAIVAVILQW
                     FLADRTQKTVSEFFGYPGISITHMMGLSGVLFAKPVNWVFDRIPVFNKIDADAETLTK
                     RFGILGDTVVIGLIIGIVVGIMAGYDVAGIGNLGIMTAAVMKIMPKMVAMFMEGLMPI
                     AEAAKEFTNKKLNGRQVNIGMDAALTVGHPTIMSTSLLLVPISLLLALILPGNKVLPF
                     GDLAFFAFAICLMIPYFKGNIVRSLVGCSLYVVTTLYMATWLAPIITDVFTLASFDVG
                     TAGLVTMLLCGLWPVGLFVLLASYFGVIGIGGLGVVLVGASIYMNKVKKA"
     gene            4122..4406
                     /locus_tag="ACGE70_002389"
     CDS             4122..4406
                     /locus_tag="ACGE70_002389"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFF8693997.1"
                     /translation="MKKLLVICGAGHATSTIAVAKLSKWLEEEGLTDQVKIYQSKIAD
                     ELNKIDEYDAVVSTTIVPDVIKAKVTNGLGLLTGIGTDAIYADVKERLGV"
     gene            4408..4923
                     /locus_tag="ACGE70_002390"
     CDS             4408..4923
                     /locus_tag="ACGE70_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HFF8693998.1"
                     /translation="MRFHHDTLTIETVSGRPSYHDIRKELLDTVDTAGIENGMLVISS
                     PHTTCSLFFEETMHDTNFFGDDYLHVDINNVMETIIPKMTSENQYNSPGPKHIDFGTR
                     LSDPNYPAEKWVMLNTDAHLRSSIYGTNSMTLIVQEGKLLLGEMGRVYFVDWDQLRER
                     TRTINLMIMGE"
     gene            4939..5601
                     /gene="rpe"
                     /locus_tag="ACGE70_002391"
     CDS             4939..5601
                     /gene="rpe"
                     /locus_tag="ACGE70_002391"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFF8693999.1"
                     /translation="MTIAPSLLNAPIAEVKELFEILKEKNVDYLHIDVMDGCFVPDMA
                     FGPSVIKDIRPLTDVVFDVHLMIDTPEKIVTRFVEAGADIVTFHIEATKHAMKVIQLV
                     KEAGAKVGITFNPATPVSFIEPVLSLVDQVLVMTINPGTENKHFIQETVVKIEQLDVI
                     RKQNDYTYDIEVDGKIDNQTIKVCSKAGADIFVSGGFIFNGKNTPQEQIDKLNQSLEE
                     VK"
     gene            5608..6180
                     /gene="hxlB"
                     /locus_tag="ACGE70_002392"
     CDS             5608..6180
                     /gene="hxlB"
                     /locus_tag="ACGE70_002392"
                     /EC_number="5.3.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0043800 - hexulose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-3-hexuloisomerase"
                     /protein_id="HFF8694000.1"
                     /translation="MTNKDQNKNYLEAIILELQNNSKRINSVELEQFSEKIVQAKHIF
                     LAGAGRSGIAMQAFTNRLMHLGFSVSLVGEISSPHSQPGDLLIICSGSGETGSLKNLA
                     AKANQSGVSIALVTMKKDSSIGQLADSVLVLPGITKDENQREEDTFSQPMGSSFEQLA
                     FLTFDSLVLSLMDKTGESSETMFARHADFE"
BASE COUNT         2117 a          878 c         1203 g         2049 t
ORIGIN      
        1 acaggagtct ggacttgact tgttcataga tgagcatttg taattaagtt atattataat
       61 aaaaaaatac gaattttagt gacgccatta tattgttttt atttttcaat tgtaatattt
      121 catattactt ccataataag gagtattttc tattggaata tcttagctct tttaaggatt
      181 tctccttttt tgtttctcat tttttaagat tatttggatt tcctttacaa caaacttcca
      241 caagtattat tgacatagat gtaattgagg gaagggggtt ccagtgctag aataaataat
      301 gaaaagaggt aacaaatgct agcgttaaga ggtgaacaag tttgattgaa agacatgtaa
      361 atttattaaa attattaagt cgacaagaag actttaaacc cgcttctttt tttagtgata
      421 aattggcagt gtcgaccaag accatttatc aagatattga attgttggaa gattatgtag
      481 ttaattacca agtaacaatt gagcgattac cttctttggg tatcaaactc ataggtgata
      541 ggaaaaacgt tgaacatttg atttcatcgg tagaaaatta tcttgaaaaa gatatttatt
      601 ctccagaaaa aagacgtgtc gagatcatta aagaagtttt gtttggtgat aagcctgttt
      661 ctttaactca tttgtctcaa atttttatgg ttagtaaaac atcgttatac aatgacctga
      721 gaattatcaa taaaattatt ggcagtggag aagttaagtt gcagtcatct gcagaaggaa
      781 ttagttttac tggtaatgaa caagcaattc aacgagcaat caagcaactt gtattctatt
      841 atgcgaatga catggaatca aaaacatttt ttcaagtttt aaatatgttg tttgattcaa
      901 aagttattag tgtcattcat gatttgctat tcaatgatta cagtgaactg actgaaaagg
      961 tttctgatta ctatgttcgg tcattgattt ctactttagt tatccaatgt aaccgtttac
     1021 tgaaaggtca tcatttaact gttgaagaag aattcctctt caatagcatt cgctatatgg
     1081 aaacgtacat tgtggcaaat ggtttaattg aagaattgaa ggcaaaattg aatcttccat
     1141 tttatgacgc agataaagaa tatctatgtc gtcaactatt tgcacatcgt gtgacaaata
     1201 atttaaaaac cagcaatgaa gagtacgcta tgagcgttca taaattgatt gaaagaatga
     1261 gtgagattga aaaaattgat ttgactcaaa atgatcgatt atatcaatcc ttattgtatc
     1321 atgtgccagc aatggtcctt cgtttaaaaa aaggtattaa aattcaaaat ccgttattag
     1381 aaaatatcaa atctcaatat tcagaactct ttagcatagt atggtacgcc atgtccatcc
     1441 ttgaagaaga atttgatgtg actttaacgg acgatgaagt ttccttaatt ttgattcatt
     1501 ttcagattgc tattgaagat cagtccaaag caaataatat tatcattatt tgccaatacg
     1561 gtatttcttc tgcgcaatta atttacaata aagttcgacg gtttttacca gcaaaagata
     1621 atgtagaaat atcaacttta gataagtttc atttagctga aattggggaa gttgatttga
     1681 ttatttcctc tctttccttt gatgcaggtg gtataccctt agtaagggta tctccattgg
     1741 taagtaaaac agattatatg aatattatgg aagcttatac taaatattta atagaagaac
     1801 cttcgcgatt tattcaaaat gaactgattc ctaaagcggt taaagttccc accatatcta
     1861 aatttgttca atcatcttta gtaaaaacag gagtacaggt ggaaagcaaa gaagaatgtt
     1921 tggatttgat gatttccgaa ctagaagcca attcattagt tactgaagag tttcgtgctt
     1981 ctatcttctc tagagagaaa atggggaata ctaatttaga aagtggtgtt gctttaccgc
     2041 atgcgaatcc atcaatggtc attgaatcca acatttttat tgtagcatta aaaaatgcga
     2101 ttaattgggg cgatcgaaaa gtcaaattag tcattatggt tagtttagct gaagaagatg
     2161 tatctgagat tagaggagta tatgaagaac tttaccaatt tattgggcaa aaagaaatgg
     2221 tcgatcgctt gttacaagtt catgatagcg aaaaactact aagaattttt caacaaaaag
     2281 gagaataaaa atgtatctag ataaagaatt atgtttattt caattagaag tgaaagatca
     2341 aaaagaattg tttcaagtaa tgtctgaaca attaaaaaac gctggttgcg tgaaagatag
     2401 tttcttagaa ggtatcacca accgtgaaca agaatttcca actggattag aagtaaatca
     2461 aattgggttt gctatcccac atacagattc cgcacatgta aatagctctc aaatctgttt
     2521 tgcctcatta aaggaaccac tggtattttc agatatgaca gataaatctc atgaaatacc
     2581 tgtacgttta gtctttatgt tggcaatgtc acaacctcat gaacaaattg atacactaca
     2641 aaacttagtt agcctatttc aaaatgaaga aaaagtaaat gaattacttg catgtaccac
     2701 gaaagaagca tttattgagt tagtacattc tgcaggagtt tattaggagg aatagagaga
     2761 tggatattgt attaaatgcc tttgaatggt ttattggatt agggtcaaac gtctttttac
     2821 cactgattat ttttgtaatc gggatattat ttggattaaa acctggtaaa gcatttgttt
     2881 ccggcgttac tgttggtatc ggttctattg gtttaggatt agttttaagt ttattatcag
     2941 atggtttagg aacagcaatt caacaaatgg gggaacaata cggtggtggc ctgaatatcc
     3001 tagatattgg cgtaggtgtt ggtggacctt tggcttactc tacatcattg ggaattttaa
     3061 tgattccaat ttcattaatt cttaacttca tatttgtggt tttgggttgg acaaaaacat
     3121 tgaatgttga tatttggaac ttttggttcg caattttcct aggtatgatt actcaatctg
     3181 ttacgggaaa cttttggttt ggcttaattg gcgcgattgt cgcagtaatt ttacaatggt
     3241 tcttagcaga tcgtacacaa aaaactgtta gcgaattttt cggctatcca ggaatctcta
     3301 tcacacatat gatgggatta tcaggagtac tatttgccaa accagtaaat tgggtttttg
     3361 atcgtattcc tgtatttaat aaaatagatg cagatgcaga aacattaaca aaacgtttcg
     3421 gtattttagg tgacacagtt gttattggcc taattatcgg tattgtagtg ggtattatgg
     3481 caggctatga cgtagcagga attggaaact taggaattat gactgcggca gtgatgaaga
     3541 ttatgcctaa aatggtagca atgtttatgg aaggtttaat gccaattgca gaagcggcga
     3601 aagaatttac gaataaaaag ttaaatggtc gccaagtaaa cattggaatg gatgcagcgt
     3661 taactgtggg tcatccaact attatgtcaa cttctctatt gcttgtacca atctcattat
     3721 tgctagcact aattcttcca ggaaataaag tattaccttt tggagattta gcattctttg
     3781 ctttcgccat ctgtttaatg attccttatt ttaaaggaaa tattgtgaga tcattagtag
     3841 gatgttcgtt gtatgttgtt acaacattat atatggcaac atggttggca ccaattatca
     3901 ctgatgtgtt tacattagca agtttcgatg tggggacagc aggattagta actatgttgt
     3961 tatgtggatt atggccagta gggttgtttg ttttattagc atcttacttt ggtgtaattg
     4021 gaataggtgg attaggagtt gttttagtag gtgcttctat ctacatgaac aaagtgaaga
     4081 aagcttaatc ataaaattaa aatacgaaag aagagatcaa tatgaaaaaa ttattagtta
     4141 tttgtggagc aggtcatgct acttctacaa ttgcagtagc aaaattatca aaatggttgg
     4201 aagaagaagg attaacagat caagtgaaaa tttatcaatc taaaattgct gatgagctaa
     4261 ataaaattga tgaatatgat gctgtagtaa gtacaacaat tgtccctgat gtaattaaag
     4321 ctaaagtaac gaatggcttg ggcttattaa caggtattgg tacagatgcc atttatgcag
     4381 atgtaaaaga aagattaggt gtttaacatg cgttttcatc atgacacttt gactattgaa
     4441 acagtttcag ggcgtccttc gtatcatgat attcgaaaag aacttttaga tacagttgat
     4501 acagcaggta ttgagaatgg aatgttagta atttcgagcc cgcatacaac ttgctcactc
     4561 ttttttgaag aaacgatgca tgacacaaac ttttttggtg atgattattt acatgtagac
     4621 atcaacaatg taatggaaac gattattcca aaaatgacga gtgaaaatca atacaacagt
     4681 ccggggccaa aacacattga ttttggaaca agattatctg atcccaatta ccctgcagaa
     4741 aaatgggtca tgctaaatac cgatgcacat ttacgttcat ctatttatgg aaccaactca
     4801 atgactttaa ttgttcaaga aggtaaactt ttattaggag aaatgggccg tgtctatttt
     4861 gtcgattggg atcaactaag agagcgaact agaacaatca atctcatgat tatgggagaa
     4921 taaacggggg aatagagaat gacaattgct ccatcattgt taaatgcacc tatcgcagaa
     4981 gttaaagagt tatttgaaat cttaaaagaa aagaatgttg attatttgca tattgatgta
     5041 atggatggct gttttgtacc agatatggct tttggaccaa gtgttattaa agatattcgt
     5101 ccactaacag acgttgtgtt tgatgttcat ttgatgattg atacaccaga aaaaattgtc
     5161 acaagatttg tagaagcagg agcagatatt gttaccttcc acattgaagc taccaaacac
     5221 gcaatgaagg tcattcagtt agtcaaggaa gcaggagcaa aagtagggat tacttttaat
     5281 cctgcaacac cagtcagttt tattgaacca gtattatctt tagtcgatca agtattagta
     5341 atgacaatta atccaggaac agagaataag cattttattc aagagacagt agttaaaatc
     5401 gaacagttag atgtaatacg caaacaaaat gattatacgt atgatattga agtagatggc
     5461 aagattgata atcaaacgat taaagtttgt tctaaagcag gggcggatat ttttgtatca
     5521 ggcggtttca tcttcaatgg gaaaaatacc ccgcaagaac aaattgataa actaaatcaa
     5581 tcattagagg aagtgaagta aaagatcatg accaataaag accaaaataa aaactattta
     5641 gaagcgatta tcttagaatt acaaaataac tcaaaacgta ttaattctgt agagttagaa
     5701 caatttagtg agaaaattgt tcaagctaaa cacatctttc tagctggtgc aggacgctct
     5761 ggaattgcca tgcaagcatt tacgaatcgc ttaatgcatt taggtttttc agtcagcctt
     5821 gtcggtgaaa ttagttcacc acattcgcaa cctggagatt tattaattat ctgttctggc
     5881 tcaggtgaaa caggtagttt aaaaaattta gcggccaaag caaatcaaag tggtgtaagc
     5941 attgctttag ttaccatgaa aaaagactcg tcgattggtc aattagcaga ttctgttctt
     6001 gtattaccag gtattaccaa agatgaaaat caacgagaag aagatacttt ttcacaacct
     6061 atgggttcaa gttttgaaca attagcattt ctcacctttg atagtttagt gttatcttta
     6121 atggataaaa caggtgaaag ctcagaaacg atgtttgctc gacatgcaga ttttgagtag
     6181 tagattttaa agatattatt tgtcaaatta agcgagacaa aactttccat ttacgtaact
     6241 caaaaaa
//
LOCUS       DAXWOO010000155         6179 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.158, whole genome shotgun sequence.
ACCESSION   DAXWOO010000155 DAXWOO010000000
VERSION     DAXWOO010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6179
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.158"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(354..557)
                     /locus_tag="ACGE70_002393"
     CDS             complement(354..557)
                     /locus_tag="ACGE70_002393"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694001.1"
                     /translation="MAAMEMKKEILMTKKHLLFGSILLGILSFSPMLLSVANKGNKKG
                     ELKLNPNYYNSQNEEVFVEKLDK"
     gene            complement(539..1072)
                     /locus_tag="ACGE70_002394"
     CDS             complement(539..1072)
                     /locus_tag="ACGE70_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFF8694002.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(1093..2037)
                     /locus_tag="ACGE70_002395"
     CDS             complement(1093..2037)
                     /locus_tag="ACGE70_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFF8694003.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
     gene            2674..2760
                     /locus_tag="ACGE70_002396"
     CDS             2674..2760
                     /locus_tag="ACGE70_002396"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFF8694004.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(2881..3570)
                     /locus_tag="ACGE70_002397"
     CDS             complement(2881..3570)
                     /locus_tag="ACGE70_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694005.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(3577..3855)
                     /locus_tag="ACGE70_002398"
     CDS             complement(3577..3855)
                     /locus_tag="ACGE70_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694006.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(3815..4729)
                     /locus_tag="ACGE70_002399"
     CDS             complement(3815..4729)
                     /locus_tag="ACGE70_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFF8694007.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(4711..5091)
                     /locus_tag="ACGE70_002400"
     CDS             complement(4711..5091)
                     /locus_tag="ACGE70_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFF8694008.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(5541..5828)
                     /locus_tag="ACGE70_002401"
     CDS             complement(5541..5828)
                     /locus_tag="ACGE70_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFF8694009.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(5848..6072)
                     /locus_tag="ACGE70_002402"
     CDS             complement(5848..6072)
                     /locus_tag="ACGE70_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFF8694010.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
BASE COUNT         1638 a         1151 c         1042 g         2348 t
ORIGIN      
        1 taaaaagtag ttttgaattc ataatcgttt aaataaaatt ataagatcag ttatttaaac
       61 agctagtaga cataatgtgt atactaccaa gtaacgcctg taccaaggga tccaggcgct
      121 gattttgtga atttttattg gtctaggtta aaagctcttt ttaaggcttg gttttctgcg
      181 gttgtttctt tctaattctt tgtgaatatt gatcggtcat tttttaaata cttgttattt
      241 ttgtttgtgt ttgttatgct ttttacatat ctcatattca tttattattc ttttctaagg
      301 ttaataatag gataatctct ttcagaaaga ctgctcgtac gagcagtctt ttcttattta
      361 tcaagttttt ctacaaatac ttcctcattt tgactgttgt agtaattagg attgagtttt
      421 agttcccctt ttttatttcc tttgttggcc actgataata acattgggct aaagcttaat
      481 attcctagta ggatagaacc gaatagcaag tgtttttttg tcataagtat ttccttcttc
      541 atttccatag ctgccaccat tttttccaat tctttgtttg ttcgtccatt gtttctgttg
      601 cttcagaaag agtcgttgaa ctattctctg aagactcttc cgtataagtc aactgcagtt
      661 gctcctgaag tttttcgatt tgttggttgg cttgcagggt gagttgttgt tgctggtcga
      721 gtagtttttg tatatgagaa ttattttgct ctttgagggc aagttgttct cgtaaaaatt
      781 cattttcttc acgcaactga tctagcaact tgtttgcaac cacgtttttg tttggtatat
      841 aagcattttt atcgttgttt tgttcatgtt tatgcaactt atctagcaac ctttgattgt
      901 ctaagttgcg agcaagttgc aatactccag aacgcaatac aaccttataa ccacgctcat
      961 ttttagctat catggaagaa tctaattttt ggatatgttt cgatattgct tgcctactca
     1021 tcttaaaaag atctgacaat tcctttattg taaaggtttc ttgtggttgc atactggttg
     1081 cctcctaggt tgctactgga ataaattatc taaatcagat aataaacttt ttttctcttt
     1141 ttccgaaagt gcggactctg tttgtttttc aagagctttt ttctcttttt ctagttgttt
     1201 gatttgttgg aggagttcaa gttctcgttt cttctgtttg attttttcgt taattgttgt
     1261 agcgggttct tcgtattctt tttgtttatc agctaaagcc aatgctttat tttggtcttc
     1321 tttggttggt gcatgtttgt taaagtcgtc tgcttttttg atctcaggtt tgaacgtaaa
     1381 ttgatagcca ataacaggtt ttccccgtcc ttttccgtat ttctttttga ttgttagtcc
     1441 tttgaagatt gcagttagtt cttgacgtat aggttttata actcgtttat ctatatcagg
     1501 ttgcttataa cttttaggaa tatctaataa ttcaagaaag tcttctttgg agaaatatgc
     1561 gaaacctttg gttctgtatt gctttaacag acgaaacata gtctttgcat agccactttg
     1621 tagctcgttg aattgttgta aagaatagcg tacccactcg tctaaattat ttaacaaagg
     1681 cagtgctttt tcgtaaattc gtatatctac gtaaggattt tctacgtccc cgttgatttt
     1741 aaattctgta aacattacaa acatatcgta agaaagccca gactcacttc tacgaccaaa
     1801 gcgtaaagat aagatttttt cataagttgc ttttaaatct tctacaaatc gtacatttgc
     1861 agttgctttg tagttactta aatcttttag ttggtcaaac gtaaaacgaa ctgtcttatc
     1921 ccctttttca cgcattctag acacgatgga aaaaaacaaa ttcatttcaa ccggtgtaaa
     1981 ttttcttaaa ggaattgtat ttaattcatg gtggtatttt acaatctcat tagccatata
     2041 atcacctcaa tccaattatg aatgacattt tttaaatgtc aagacattca tatttgacgt
     2101 tcaaacagac aaaatgtcct tcataaacag acaaaatgtc cttcataaac agacaaaatg
     2161 tccttcataa acagacaaaa tgtccttcat aacattctgt atccattggt agagtaagga
     2221 cacagcttac ggtaaagagg tttaaaaaga ggtttaaaaa gagatataaa aagagggggg
     2281 ctaattttcc cgcctaaaaa ccacttcttt tttctttaaa caggttcgcg catttttcaa
     2341 aactgccaat ccaaaacctt gcttcctacg gtcgccaaaa aggtgtgatc gctacgctct
     2401 cacattgtac tgcgcagtcg cctaaaggct cctttgctaa aaacatgttg gtaaagaggc
     2461 ctttgagcgg ttctgaatgc ttatctaagc cgttttaaga cagtcttgga taaaaatcct
     2521 tatctagctt taaagcgcct tacaggcggt cagggcaagc cctaacaacc ctaaaataag
     2581 cgatacttaa aaacaccaca caagtttaat tttttaagat ataataactt gtcgcctatc
     2641 cgacagtctt gcgactggaa ggaggtgtat ctaatgtttg aattgttcat ttgccccctt
     2701 cttatagaag tttgtgtctc tttattcagt tattggttga ataaccgaaa caagaagtaa
     2761 aatcaataaa ggcgacatca acctgaaaaa aagccccaga gaatccgacc tctctggggc
     2821 tttcgtgtat ctaatgtttg atcatttgca tgtttagttt aacatgattg ccgatcaaat
     2881 tcaattttgc tgttgctgat ctaaccattc cttgacactt tttagataat ttaatgccgt
     2941 ttcttttttc gttggatttt gtatgttcgt tgcttgctga agcagtgctt tataacgttc
     3001 gtaggtaact gattgttgcc gttcttttcg ttcctgttca ctagttgaaa aagggattgt
     3061 atcactgaat aaattataac aagcttcttc tgtctgcgta tctttaaaat attgcacgtc
     3121 ttttttggtt cgctcatagt tgtcttgaac ctctttttgc tgtttttctg gcaagttttg
     3181 ataaagacga gtgagttttt ccatgttttt atccgtcaaa cgtttttctt gattcacaaa
     3241 aatatcgttc atttcctcat aacaagtggt aaactcttcc attttttgtg ttaacacttg
     3301 ctcctttttc tttttatcac atgctgcttc atacttttga gtgatatccc ctaataaaat
     3361 acttcttgaa atacgtttat ttccctctaa aacgccgaat aaaccactta aaacgattga
     3421 taatgtaaat ataacccctg gcaataaaaa cctcttaaat cgaattttag agccttttaa
     3481 tgaatatctt gtttgataag ccttatagct aagccaaata attcctaaaa tactggcaaa
     3541 aatgagtaga tagggtaaat agaatgccat ttctatttat tctccccata ccagccaaaa
     3601 ggctaacagt gtcatgatta agcaacataa gggggcaaga atatccattg atcgctgcat
     3661 aattactttt ttctgttctt tatttcgctg gtttaatagc gcatggtgga attttttgac
     3721 ttgtgaatca attgtagttt gtattctttc tttggtataa ggtgcaagaa aagcacgttg
     3781 tttctttatt tcattttcta tgtcttgtaa cgttttagaa cgataattct tgttggtagt
     3841 ctgctcctct ttcaacgtgt tggtcatggt ttcttcgagt cggtctaatt tccggttgtt
     3901 cagtcgtaag agttcgttgt tctcttcgta cttcttttgc agtttcgcga atgctagctc
     3961 gagctcttcg tagctcgttg gttcgttgtt gattgtaggc tcgttctcgt ctgtccagct
     4021 catgtaaaat cgtctccttt tcgtaatcct ttcctagttt gttggatcgt acttttttct
     4081 ttttgtggat caattcgtaa gtaaaatttt tcccacgatt caagcaatga atgccgaatg
     4141 ttttaagaga atcacaaaat tggttatacg ttcgagtttt tttgtttttc attgtttgat
     4201 caatcgcaaa ccgaatttcg tctttccaaa caggttcatt tttttctttt aatttgattt
     4261 cagacatggt tcgtttttcg ggtttcttcc ctcgttcagt gattgttaag tcatgttcaa
     4321 gacaaatctc gtcatttagg tcataacatt tgtttaaaaa tgcttgacca tgggcttgaa
     4381 atttgttccc agtttccaga ttgacggagt taatgacaat gtggttatga atatggtctt
     4441 tgtctgtatg tgtgtaaatc gcgatttggt ggtcaggggc tatttttttc gcatactctg
     4501 cccctagttg attggctttt tcagcggtaa tttctcctgg tcgaaaagac tggatcaata
     4561 agtgtgcttg tacacgatcg tcttttccat acagcatgcg aatttgtttc atttcgcttt
     4621 tggcataatc aatgtcacag tttatccctg ttttgacggt tgctcgtggt tcggcgtaat
     4681 tgatcgctcg gctacaagaa gtggttcggc ttactttaac tgttgccata tcttttgtag
     4741 ctccttttgc atgattttta ggttttcgga taagcgttgg gcgtcgccag gatcaacgtc
     4801 gtgttggtgt gtgttgcacc acttggcaat ttgattgata ttcccgccag ctttggctaa
     4861 atcgcgcgcg attgcctgag caatgtctgg cgccactttc ggtgtcacca gacgcgcgtt
     4921 ttgtgccttg gctttcacga aagccggcac actcatttgc aaagcttcag ctgactgctt
     4981 cagctttgca aattcggatt cgctcactcg aaaactgatt tgttttggct cggatcgata
     5041 ggtatatttt ttagaatgat tgctagccaa atattgattt tgttcgctca taagtcccac
     5101 cctttcagta cgtcctacgg acgtatcgtt cgcaatacca ttgtacctaa aaaggtacaa
     5161 taagtaaagc tcactaaagc tcactaaagg gcttggcaag ctctatattt gctagccaaa
     5221 tacctacgta tttggtgcaa ataaccgctt gtcaaagggg cgcaagctcc cccttgaatc
     5281 cccctccccc ccactctcca caatacaaaa aagactagca aatgctagcc actttttgta
     5341 aagtttcgct tgtttcacct tcggtgaaat tgttgcaaat caataattag gtagagctac
     5401 atttttttcg ttgttcaaag attcttatgc catttactat taataaatct ataaatatga
     5461 taatttttcg gctctataat ttatgggact gatgaatcag aattgattct tcagtcccat
     5521 tttcatttta ttccaaagaa ttaactccaa ataccaatag aagcccatcc tctatatact
     5581 tgagattcct tactaacaat ttcttgtaat tctattaaag atttaggtaa tccaaattta
     5641 tgttttaaaa tatatcttcc aagaaaacta cttaatttca tactaacttc accaacactt
     5701 tttccctcac tgatttcttt agaataaaga aagagctttt cttttaagtc aggatagtca
     5761 tctatagttg cttcattgta tactttacta attgcattta aaagtttttc ttctttagta
     5821 aaatccatta taataacctc cctaccacta ccttctaggt gcccatggac catgattaac
     5881 ccaaccatta acaataattt ttccaatttc tcctttagct tgattccaat ctacccaaca
     5941 tttttctttg ttacaataaa gaccatttcc atagtaagta gctgcatcaa cttttattcc
     6001 tgtaccgatg ccagctagac aagttcctaa tattcctaga ataacacaat gttttaatac
     6061 tttctttttc atgattgttt ttccttttcg tttttttaaa attgcttgtt atattaaata
     6121 atcttagtaa ttttattata taacatttta ttatataaca atgattaacg tcataaaaa
//
LOCUS       DAXWOO010000156         6062 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.159, whole genome shotgun sequence.
ACCESSION   DAXWOO010000156 DAXWOO010000000
VERSION     DAXWOO010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.159"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            37..2634
                     /gene="adhE"
                     /locus_tag="ACGE70_002403"
                     /gene_synonym="adhC"
     CDS             37..2634
                     /gene="adhE"
                     /locus_tag="ACGE70_002403"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFF8694011.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            2863..3969
                     /locus_tag="ACGE70_002404"
     CDS             2863..3969
                     /locus_tag="ACGE70_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFF8694012.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            3986..5010
                     /locus_tag="ACGE70_002405"
                     /pseudo
     CDS             3986..5010
                     /locus_tag="ACGE70_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            5016..5495
                     /locus_tag="ACGE70_002406"
     CDS             5016..5495
                     /locus_tag="ACGE70_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFF8694013.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKARLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            5511..>6062
                     /locus_tag="ACGE70_002407"
     CDS             5511..>6062
                     /locus_tag="ACGE70_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HFF8694014.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVG"
BASE COUNT         1949 a         1074 c         1367 g         1672 t
ORIGIN      
        1 tttaacaaat aaccatgacc cattaggagg ataatgatgg ctaaagtaat agaaaaagaa
       61 aaaacgaaga caattgacgt acaagcaatg attgatgatt tggcagaaaa agcaaatgca
      121 gcattgaaag aaatggaaag ctttgatcaa gaaaaagtag accatatcgt gcatgaaatg
      181 gcaatggcag ctttggatca acatatgcca ttagcgaaga tggctgtaga ggaaacaggc
      241 cgcggtgttt acgaagataa agcaatcaaa aatatgtatg cgtccgaata tatttggcat
      301 aatatcaaac atgataaaac agttggtgtg atcaatgaag atgtccaaaa agggttgatt
      361 gaaattgctg aaccagtcgg tgtggtttgc ggagtaactc caacaacgaa tccgacttcc
      421 acaacgattt tcaaatcaat gatcgcgttg aaaacaagaa atccaatcgt ttttgctttc
      481 catccaagtg cccaaaaatc ttctgcagaa gcagcacgag tggtacgtga tgctgctatc
      541 gctgccggag ctcctgaaaa ttgtatccaa tggatcgaac atccttcgat cgaagcgact
      601 tctatgctaa tgaaccatcc aggtatcgcg atcgtcttag cgacaggtgg agctggaatg
      661 gtcaagtctg cttactctac aggaaaacca gctttaggtg taggtccagg taacgtacca
      721 gcttatattg aaaaaacagc aaaaattaaa cgtgcagtga acgatttgat cgtttcgaaa
      781 acatttgata atggaatgat ttgtgcttct gaacaagctg tgatcgtgga taaagaaatc
      841 tatgcagcag taaaagcaga attccaagca caccaagttt atatcgtaaa accagatgaa
      901 ttgcaaaaat tagaagatgc agtgatgaac gaaggaaaat atgcggtcaa tccatcaatc
      961 gttggacatt ctgcgatgga aatcgcaaaa ttggcaggaa tcagtgtacc aaaaggaacg
     1021 aaaatgctga ttgctgaatt ggaaggcgta ggcccagatt atccgctatc aagagaaaaa
     1081 ctttctccag ttcttgccat gatcaaagct aacaacacgg accatgcgtt tgatttgtgt
     1141 gaaggtatgt tgaatcttgg aggattagga catacagcag ttatccattc agaaaacgaa
     1201 gagttgcatg tcaaattcgg tcttcgaatg aaagcttgtc gtattttagt gaatacacca
     1261 tctgcagaag gcggcatcgg tgatatctat aacgaaatga ttccgtcatt gactttggga
     1321 tgcggttctt atggtaagaa ctctgtttct cgtaacgtct ctgctgtaaa cttaatcaat
     1381 gtcaaaactg tagcgaaacg gagaaataac atgcaatggt tcaaattacc gccaaaaatc
     1441 ttctttgaaa agaactctct attgtactta gagaaaatgg aaaacgttga acgtgtcatg
     1501 atcgtttgtg accctggtat ggtccaattc ggttactgcg atacagttcg tgaagtattg
     1561 gcacgacgta aaaatgatgt gaagatcgaa gtcttctcag atgttgaacc gaacccatcg
     1621 acaaataccg tatacgcagg aacgaaactg atggcagact tcaagccaga tacagtaatc
     1681 gctcttggag gcggttctgc aatggacgca gctaagggaa tgtggatgtt ctatgaacac
     1741 ccagacacat ctttctttgg tgcaaaacaa aaattcttgg atatccgtaa acggacatat
     1801 aagatcgaca aaccagaaaa aacacaattc gtatgtatcc cgacaacatc agggactggt
     1861 tctgaagtaa cgccatttgc ggtcatcact gacagtgaaa cacatgtgaa atatccgtta
     1921 gcagattatg cattgacgcc agatgtcgca atcgtcgatc ctcaattcgt catgtcagtt
     1981 ccagcatctg tcacagcaga taccgggatg gatgtgttaa cacatgcaat cgaatcctat
     2041 gtttcagtca tggcttcaga ttatacacgt ggattgagcc tacaagcaat caagttagtc
     2101 tttgactatt tagaaaaatc agtaaaaaca cctgatatgg aatcacgtga aaaaatgcac
     2161 aacgcttcta caatggctgg tatggcgttt gctaacgcat tcctaggtat ttgtcactca
     2221 gtcgcacata aaatcggcgg ggaatacggg atccctcatg gacggacaaa tgcgattctt
     2281 ttaccgcata ttatccgcta taatgcaaaa gatccacaaa aacatgcgat gttccctaaa
     2341 tatgactatt tccgtgcaga tacagattac gcagatatcg caaaattctt aggtctgaaa
     2401 ggcgaaacaa cagaggaatt agtcgaagct ttagcaacgg ctgtttatga attaggaaaa
     2461 tctgtcggca tcaacatgag tctaaaagct caaggagtca cacaagaaac actagataca
     2521 accgtagacc gtatggctga attagcttac gaagaccaat gtacgacagc taatccgaaa
     2581 gaaccattga tctctgaatt gaaacagatc atcattgatg cttacaacgg ataagcaaaa
     2641 gaaagcgtag aaaaaagata agaaaataga tgaaaaagaa cttggaaaac gttgtttctc
     2701 caagttcttt ttttaatttc caagtaactg aaagttactt ttgttattta acatttaggt
     2761 caattttaga cagtttttgt tcaaaaagcg atatttatag tggacggtat tactgctata
     2821 ttaaggatat aaaatgaaag cgtttaaaaa ggtggcgtaa agatgattat taaagaagag
     2881 atttttgtac ctaaagaatt agtgaaagaa aaaattgacc tgctcgttga aaacctgaca
     2941 acgatcaaag acgataatgg tgaatttttg ttggactttg atggactgaa agtagatgac
     3001 aaaagctgga cagtgtggaa ttggccacaa ggtgtcggtc tatatggtat ttataaaaat
     3061 taccagatga caaaaaatcc tcgcgcttat caagtggtca atgaatggtt tgaggatcgt
     3121 atggaagaag gcgctccgcc caaaaacgtc aatacaatgg caccgctatt gactatggct
     3181 tacttgtatg aagacacgaa agattcacgc tatttgcctt atttagaaca atgggcagaa
     3241 tgggtcatga atgacatgcc tcggacaaat gaagacgggt tacagcatgc cacatatgga
     3301 ccggaaaata agaatcagtt gtgggacgat acattgatga tgacagtgct tcctttggca
     3361 aaaatcggga aactgttgaa tcgcccagaa tatttagaag aagctcgtta ccagtttatg
     3421 atccatatca aatacttgat ggataaacga acaggattgt ggtatcacgg ctggacgttt
     3481 gaaggaaatc ataactatgc cgaagcattt tgggctcgtg gaaactgctg gttgacgatt
     3541 gctattccag agatcattga aatcttagaa ttatcgaaag aagatgcatt gcgcaaatta
     3601 ttgatcgaaa cacttgaagc acaagtgcgt gcattaaaaa cttatcagtc tgaaagcggt
     3661 ttatggcata cgttgttgga tgatccttct tcttacgtag aatcttctgc aactgcagga
     3721 tttgcttacg gtattttgaa agcggtccat aaacgttatt taccacaaga atataaagaa
     3781 gtcgcttatc gtgctatcca aggattgctt gaacaaatcg atgaaaaagg agaagttcaa
     3841 aacgtttcaa tcgggacagg aatgggggac agtttagatt tttatcggaa tatcggaatc
     3901 acagcaatgc cttatggaca gtcgttaacg gtgctttgtt tgacagaatt gctcgtctct
     3961 tattgttaaa agaaaggaaa cccatatgaa aaaaacatca ctgctgtttg cagcaagttc
     4021 gcttagccta ggtctgctag gtctaagcgg ctgcggaaat aattctagtg tggcaactgt
     4081 cgcagatgca gacgtaaatg agatagttac cttgcgcttt gcttatgcaa gcaacagcca
     4141 gtaattgatg caatgaatga atttgggcgt ttagtaaaag aaaaaacaga tggttccgta
     4201 cagatccagt atttcccaga tggacaactg ggtggagaac gtgaactgat cgaattgaca
     4261 cagtcaggag cagtagattt cacaaaagta agtgcctcag cgttggaaag tttctctaaa
     4321 gattattcga ttttttccgt tccttatcta ttcaatgatg aagaacattt ctttaaggtg
     4381 atggataatg aagaaataat ggagccaatc taccaatcga ccaaaaattt aggatttacc
     4441 ggattgacct attatgattc tggtcagcgc agcttttata tggtagacgg tccgatccat
     4501 acgccggaag atttgaaggg aaagaagatc cgtgtgatgc aaagcgaaac cgctatcaaa
     4561 atggtagagc ttttaggcgg ttcgcccgta cctatgggaa gtgatgaagt gtacacgtct
     4621 cttcaatcca atttgatcaa cggatcagaa aacaatgaat ttgtcttgta tacagctgga
     4681 catggcgggg tagctaaata ctattcctat gatgagcata cacgtgtgcc agatatcatc
     4741 atcatgaatg atgccatcaa agaacagcta actgatgaac aggaaaaagc cattgaagaa
     4801 gcagcgaaag aatcaacggt ttttgaaatt gaagcttttg caaaagcaat tgaagaagaa
     4861 aaagctttag cacaagaaga acatggagtc caattcaatg atgtagataa tacaccgttt
     4921 cgagaggcag ttgccccact tcatgatata ttcaaaaacg atcctaccta caaagacctt
     4981 tatcaaaaga tccaagagca aggagcgtag ctgtaatgaa aatgatttta aacaaggtat
     5041 tagagatcct gggagctgtt ttgcttgttg ggatggtcgc tgttgtttta tggcaagtat
     5101 tttccagaac ggttttagga aatccaaata cggtcacgga agagcttgtt cgattcggtc
     5161 ttgtctggtt tgccatgttg tcttccgcgt atgttgtggg acaaaaagca cgtttagccg
     5221 taacggtttt aagtgaaaaa ttaactggca aaaaaagtaa aattttagaa gttgttgttc
     5281 aaagtttatt tctggtattt gctgcgatga tcatggtgta tggcggatgg aatgctgtca
     5341 cattgacaat ggggcagatc tcgccttctt taagtattcc tatgggatat gtttatctgt
     5401 cagtacctgt atcgggggta ctcatcatta tctatagcct gatcaatcta atcgatacat
     5461 taagcaacaa gccagcaatt caagaaaaaa tataaaaatg gaggaataaa atggcaatag
     5521 aagccggatt agtgttattt tttgtatttg ttgtgttgct tgtattaggg atgccgattg
     5581 ctataagtat tgcattctca tcgatgatga ctttattgtt agttataccg tttgatgttt
     5641 ctgcttttag ttcagctcaa aaaatggtgg gaagtatcaa tagtttttcg ctgatcgcta
     5701 ttccgttttt cgttctctcg ggcattatca tgaataatgg agggatagct aagaaactag
     5761 tggattttgc gatgttgttc gtcgggcgcg tgccgggggc tttggctcat acgaatgtgt
     5821 tagggaatgc tttatttggt tccatctcta gctcggccat tgccgcttct accgcgattg
     5881 gtggagtgtt gatccctcag caagtggaag aaggatatga ccggaagttt gcgacagcgg
     5941 tcaatatcgc gtcagctccg acaggtatgg tcattcctcc aagtactgct tttatcatgt
     6001 attcacttgt tgctggtggt gcttcgatct ctgctttgtt tatgggaggt tatctcgtag
     6061 gc
//
LOCUS       DAXWOO010000157         5942 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.160, whole genome shotgun sequence.
ACCESSION   DAXWOO010000157 DAXWOO010000000
VERSION     DAXWOO010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.160"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            196..921
                     /locus_tag="ACGE70_002408"
     CDS             196..921
                     /locus_tag="ACGE70_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694015.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHSQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            911..1420
                     /locus_tag="ACGE70_002409"
     CDS             911..1420
                     /locus_tag="ACGE70_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694016.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(1490..2938)
                     /locus_tag="ACGE70_002410"
     CDS             complement(1490..2938)
                     /locus_tag="ACGE70_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFF8694017.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(2938..3654)
                     /locus_tag="ACGE70_002411"
     CDS             complement(2938..3654)
                     /locus_tag="ACGE70_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFF8694018.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            complement(3635..3985)
                     /locus_tag="ACGE70_002412"
     CDS             complement(3635..3985)
                     /locus_tag="ACGE70_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFF8694019.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            4129..4902
                     /locus_tag="ACGE70_002413"
     CDS             4129..4902
                     /locus_tag="ACGE70_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFF8694020.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMDNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            5073..5439
                     /gene="ssrA"
                     /locus_tag="ACGE70_002414"
     tmRNA           5073..5439
                     /gene="ssrA"
                     /locus_tag="ACGE70_002414"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            5682..>5942
                     /locus_tag="ACGE70_002415"
     CDS             5682..>5942
                     /locus_tag="ACGE70_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFF8694021.1"
                     /translation="MLMEGKTQLWFKFDPSNRFIKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKDVYFLFDVVTDVPDARSDHRRYDY"
BASE COUNT         1718 a         1166 c         1034 g         2024 t
ORIGIN      
        1 aaaattattg tcgctgctcc tcttttcgaa taaacagtgt tcaaaagctt gcatctacgg
       61 taatagaata gggcagcttc ttcacaacct cttgcttttt acaaaatatt ttctggtttt
      121 aattcgtgat ttcactaaag gaatttcata ctttattcat attcattttc tatacttttt
      181 atagaggtga aaagtatgaa aataatccgc tatgctactg ctcgattaat ttattacaaa
      241 aagcagacaa tactctattg tttattttca agcttttctg cttttttatt gatagcatgc
      301 ctgactcttt accattcgca atcggcttta gccaatcaag tccaagatcg gctttcattt
      361 attggcgctt ctgcatctac agagcttctt ccggctttag aattagctag ccgattttat
      421 ataaaaggca tattcagttt attttttcta tttgccttcc tttttatctt tttcttttac
      481 tattcagtca ggcaaaacaa gcaggaattg atcaattggc gcctgactgg tctttctaaa
      541 accaaattat tcctttttat tttctggcag ctgttctttc ctctctttct ttgttgtatc
      601 ttggttttat tgtggatcgt cagcttccag tcagtttatg aagcgcttct tcaaaggatc
      661 aattttcttg tcttaaagac tactgaactg ccagatatcc gtgatatctt gacttcggta
      721 agcggactta caattccgat ggatcaacag acttttttta ccatcgattt ccaaaatgat
      781 ctttttttca cagatatact taagggattt gcccaaactc ttattgggct cagtggatgc
      841 tgtctcattc ttagtctatt ccctttcact ttttttatcc atcatttgca aagtaggagg
      901 cctggacgta atgaaacctg aatatttgat tcatgctgaa cacttgaccg ttattttgtg
      961 tcctctcgaa aaacagcata aaatattgga gcacttgaaa aaagaggttc ttgttgcaaa
     1021 actggataat tttgcgatca tccaaaaaaa ttggccgatg tttccttatc tgaatcttaa
     1081 agaccatgtc ctgttggatg taccggaaaa agaaatcaaa caagatcgtt tggcctatca
     1141 agaaaaactt gatatctctc cttccttgct taattgtgcg gcagctgagt tgacgtcttt
     1201 tgaaaaagta aagctgcaat tacttcatgc cttgctttcc aagcgaaaca atattgttat
     1261 tgaagataca tttgatgaac ttagtgtatt ggagatccaa gagctactcc acttgctttc
     1321 ttatttagct catgaagaaa atcaaggtat cttactcttt actcatgatg ctactattgc
     1381 tcaaagtcct tatattgatc gtttagagcc cgcgggctaa atcaatcatt ttatagtaaa
     1441 aacaagcggt atagaagatc gattcatgat cttttatacc gcttgttttt tactcttttt
     1501 tgatttctac gtcaggcaaa cgaacagcga attttgttcc ttttcctagt tcgctttcta
     1561 cttctaattt tcctttatgc aagcgtacaa gttgctgcac gattggtaaa cctaatcctg
     1621 attcaccata ttttgtattt tttcttgaag gatctacttt ataataacga tcccagatac
     1681 ttttcatttg ttgttcagac attccaattc ccgtatcttc aatcgtgatg atcgtttcaa
     1741 gatagccttt ttctaaagta atcgtgattt ggccgttttc agtaaactgt atcgcgtttt
     1801 gaataatatt gaccatgatt tggacaaatc ggtcataatc agcgtaaaca tcaatatcat
     1861 caacggcttt caatgtcaat gtatctcctg ctgcttctgc tttagcttct aactgtgcaa
     1921 caatattttt caatgtttct gttccattga attttttaat gaccatactg atttgattcg
     1981 tacggatttt ttcataatcc aagttttcat tcaccaaacg gatcagacgc tctgtttcat
     2041 ttttcatcaa tttaatcgcg ttttctcgct gattttcagg gattgcattg tattctaacc
     2101 cttccagaag tccgttgatt gtcgtaagag gtgttctcat ctcgtgagaa gcatctgcca
     2161 taaattgtct tcgacgattt tcttgttctt caatttctgc ttgagattct ttcaatgaat
     2221 tcgtcatttt attgaaatca tctgctaact catcaaattc atctttgtca tgtacgggga
     2281 gctgtacatc aaaattacca ttcgtcactt ctttcgtcgc attccttaac cgattgatac
     2341 gcttaacttg gaatgctgca aaagcatagc tggcaataac tgcaacgata ctggaaaaaa
     2401 taaatccttt gaacaggttc agtgtaattg gacgaacgct gttatcgata tttcgcgcag
     2461 gttgactaac tacaagtgct ccatagaatt catgattcaa attaaatggc acaagtgcgt
     2521 aagaggtcgc ctgtgcttgt cctaaaatat ttttattcga tgtgaatttt tcttgacgtc
     2581 cattcctcag atttttccat tgatcagctg ttaaggtaaa attcagtaaa gctgtattag
     2641 tcggataaat gacacgttcg tttttatcga taaatataaa gttcacattt tgttctgtca
     2701 gtacttgctc tgtcagcagc aatgcatttt gaaacgcctg atcttgacta aactgcggga
     2761 gcgtatcggc ataggtttgc gttgtctttt caactgattc agcatatcca aacagttgct
     2821 ggtaattgtt ttcttcaatc gtttgttttg tcagttgtgt aaaagaaact ccaacgatca
     2881 ataagatcaa cgcgaccatt ccgcaaaaag ctaagagttg ctgatacaaa taacgcatta
     2941 ggcaacacca gaatcatcga atttgtagcc gactccccaa acagtttgga tgacttgtgg
     3001 tccgaccttt tcgatttttt gtcgtaattt cttgatatgg gcgtccactg tccgttcatc
     3061 accaaagtac tgataatccc agactaattc aagcaactgc tcccgagaga atacttgacg
     3121 tggttttttt gctaaagtaa acaatagatc gaactcttta ggcgtcagtc cttcaattgg
     3181 ccggttgtcc agatacgctt ctcgtgtttt cgtattcatc ttgaaatgac tagtctcgat
     3241 atcaaaatga tcatctgaaa ctgctgcttc ttctcttgct tctgccaact cactacggcg
     3301 atgcaaagct ttcattcgag caataagcgt cagaggactg aaaggttttg tgacataatc
     3361 atctgccccc atctctaagc cgatcacctg atcgctttca gaatctctag ctgtcagcat
     3421 gataatcggc acagtacttg aaactttgcg gatctctctt cctactagca taccgtccag
     3481 tgtgggcaaa ttcaggtcta gtgtaatcat atcccaatgt tgtgggtttt ctaaaaaggc
     3541 atcgagccct tcttttccat catacttgaa agttgcttcc caaccttcat ttaagaaaaa
     3601 catctgcatc atttcagaaa cagattgatt atcttcaatc attaaaatat tcattatcgt
     3661 tccctccagt ttccaggtca tatttctgta atttcttttc tgtcataata aaaataggca
     3721 taattggaaa tactagcaga agcatttcaa tcagaaaata attcgtatgc ccagatgtct
     3781 ttaaataata gccgatcaat gccatcagta agcccataag aaaaaaccag tttccgcttg
     3841 tcttttgagc cagccgccaa tgagcgtctg tttttgcggc aagaggcgaa tgatacccat
     3901 aaaaaggcag atttcctttt gagggcagaa tccgaaaaag actgccaatt attaagaata
     3961 agataccaat actgaaaaaa atcattcttt ccctcctaaa ttctcttctt atagtatact
     4021 ctaaattgtt gactgaacca atatgttcag gaattcttca cgaaaggttt gtcttttgtt
     4081 cattctttgt taagataaac ttattccaat aaaaaaggag ttttatttgt gtcaaattat
     4141 cgtgcaatca ctttctttga tcttgatgga acattactag atgcgaaatc aaaaatcaca
     4201 cccgaagtgg caaaagctat gaacgcatta aaagaaaaca atgttctccc cgtcatcgct
     4261 acaggacgta cagaagcaga aatccactct atcatggaag atgcgggcat tacttctgcg
     4321 gtcaccatga atggttcttt cattcgagta gacggcgaag aaatctattc tcaaacattt
     4381 tcttcagaag aatgtcaaaa aatgtatgat catgtacagt tgaacggtca tgaactttct
     4441 tactataacg atcagaaaat ctggtgtacc ggtcatagcc aaacaatgat caatgcttac
     4501 gacttcattc attcagatgt tccggaaatt gatcctttag gctttaaaga aaacacagta
     4561 aatatgttgc tgatcttatc tcaatcaggt gacgaatact atcacgaaca ttttcctgag
     4621 ctaacttttt atcggaatgg tccttactcg atcgatacag tgaagaaagg aatctcaaaa
     4681 ggggcaggtg tcaaacgtct gcaaaaagaa cttcagctgg aaaaagttcc tacttttgga
     4741 tttggcgatg gtccgaatga ttttgccttg ttggaagctt gcgacaacaa aatcgctatg
     4801 gacaacgcct atgacggatt aaaagaactt tctaccttta tcacaaagaa aaatacagaa
     4861 ggcggaattg tccacgcatt gaagtatttt gatctgattt aagatctttt tttgaagcta
     4921 cctcttttct taagaaaatc gcttattttt tcttaagaaa ggggcagtta ttatataaat
     4981 aatgggatat gattcgcttt tttattgaat ttatcccaat atatgctata ctaattttgt
     5041 atgaaaaata cacgtaaccc ttgttagcta acggggacgt tacggattcg acaggcacag
     5101 tcgagcttga attgcgtttc gtaggttacg tctacgtaaa aacgttacag ttaaatataa
     5161 ctgctaaaaa cgaaaacaac tcttacgctt tagctgccta aaaacagtta gcgtagatcc
     5221 tctcggcatc gcccatgtgc tcgagtaagg gtcctaactt tagtgggata cgtttcaact
     5281 ttccgtctgt aagttgaaaa agagaacatc agactagcga tacagaatgc ctgtcactcg
     5341 gcaagctgta aagtgaatcc ttaaatgagt tgactatgaa cgtagatttt taagtggcga
     5401 tgtgtttgga cgcgggttcg actcccgccg tctccattgt gagatttcgc cagatttcgc
     5461 tagatgctta aatccttatt ttataagggt ttgagcattt tttgttttcg ctgattttcg
     5521 ttaaatgaat gaaaatggca ttcaaaatgg cattcaaaat ggcattcaaa atggcattca
     5581 aaaaaacatc ttttaatata tatgttccat ccatattgcc aacgctattc ctgctttaca
     5641 aaaagaacat tagttcgtat actctttttg aggtgaacat tatgttgatg gaaggaaaga
     5701 cacaattatg gtttaaattt gatccttcga atagatttat caaagatttt tataaagtat
     5761 gggattcaga agttttcttt ttagcaatcg aagatagctt attaatcaat ctctactatt
     5821 ctaataagaa ctatttcaaa attcctgctg cgaaaactag aatgaagaaa gacgtatatt
     5881 ttttgtttga tgtcgtgact gacgtgccag acgcacgaag cgatcatcgg cgttatgact
     5941 at
//
LOCUS       DAXWOO010000158         5875 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.161, whole genome shotgun sequence.
ACCESSION   DAXWOO010000158 DAXWOO010000000
VERSION     DAXWOO010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.161"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(170..3319)
                     /locus_tag="ACGE70_002416"
     CDS             complement(170..3319)
                     /locus_tag="ACGE70_002416"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HFF8694022.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEE
                     QIGVYETVKGSLMEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDNLIHA
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(3333..4550)
                     /locus_tag="ACGE70_002417"
     CDS             complement(3333..4550)
                     /locus_tag="ACGE70_002417"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFF8694023.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(4540..>5875)
                     /locus_tag="ACGE70_002418"
     CDS             complement(4540..>5875)
                     /locus_tag="ACGE70_002418"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFF8694024.1"
                     /translation="LGYSISPEYLFNVLADQAKQATFQLNDLNKAFVQLASTYNQFNG
                     LFDDVDLQSKKLGTDEQQRNVTITEVIKKLNDVEVLGHDGDVIGDAYEFLISQFASEA
                     GKKAGEFYTPHMVSDMMAQIVTLDQKERRFFSVFDPTMGSGSLMLNVRNYLTHPDNVK
                     YHGQELNTTTYNLAKMNLILHGVDAEEMNLRNGDTLNKDWPTDEPYTFDAVVMNPPYS
                     ANWSADTTFLDDSRFNRYGKLAPKSKADFAFLLHGFYHLKETGTMAIVLPHGVLFRGA
                     AEGVIRQKLLEDGSIYAVIGMPANLFFGTSIPTTVIVLKKNRQTRDVLFIDASREFVK
                     GKNQNKLSEENIQKILETYAERKDVEKYAHLATFDEIKENDYNLNIPRYVDTFEEEEP
                     IDMVHVGNDIKKIRQEQQVLEKELLEAISSLQTTPENEAWLQGALEVFKHEQ"
BASE COUNT         1605 a         1251 c          983 g         2036 t
ORIGIN      
        1 tgcaacagaa ccggtaaatg aatgaaaaag atggaagaat cattattcaa tagctttctt
       61 aaatactccg ttataaaatg tgaacaagta aaaagacatc tatccctatt ttctacactt
      121 ccactttact actatctttg aatcgtacaa ttttcttaac tcttctcgtt tacctcaatt
      181 cattttcaag tggaatcaac tcatcatcta acattttacg ccatgctcgc ttcatttctt
      241 gtgggtattt aaatggtttc gcttctggat gtttggcttt atatccttcg taatccttac
      301 tgttgataat tgctttcata ttaggaattg gatcttcacc tggcatatat tgcagattag
      361 aagcatgtaa ttctttctca gaaacaaacc actcattgga aaaggcatga atcaaattat
      421 cacttgcttg tgtaaaatag cgacgtttaa tgacaaagat atcttcatta ctatctaaag
      481 tccccacatc aaatgcattt aaaatttgtc ggtaaacttc tttgactcct tctgttttat
      541 tcaatttagc caaagctttt tcaatttctt ctcgtgcatc tgaactattc gcagcataag
      601 ttcctagtaa ttgatcaata taagtagaat caatatcata caacttcgtg gtattttcac
      661 tataaaattc gatcgttgaa aaatcttctc cctctttttc tggttcttcc tccatcaatg
      721 aacccttcac ggtctcgtac acgccaattt gttcttccaa aagttgaatt tgattgttca
      781 aagttgaaga ttgcaccatg tcatcattgt aatcatcata agtcactaac gattcatacg
      841 cacgattcaa ttcttgaaat gctttgacgt actcgatacg gatttcaagt ggcgtatgtt
      901 catcaatgga ttccatggat gaaacctctt ttaacttacc atatgcttgc ttgaatcttt
      961 ttttggattc ttggtaactt ggataaatca aacccgattc ttctttagct tctgaataca
     1021 gttttgtcgc atcctcgaca ttcttctcca ttgtcgctgg ctttcggaaa gtaacaatta
     1081 gtccttttgt tttttcagga tacgtacggt tcgtacgtga aaaggcttgg atcaatcctg
     1141 catactctag attacgatcc acaaataggg tttgaatcgt aggggcgtca aaacctgtca
     1201 atagacgatc cactacgatt actaaatcga tttgtttccc aaattgtttg aattccgctc
     1261 tttttcgtgc caaacgatta ttgatgtctc cgttataacg ctcaatgtct gctagtgacc
     1321 aggcagtatc ataatactga ttatattcct caataatttt ttgcatttct tcttgttggg
     1381 cacttgaatc tcggctattt tcttctaatg aataggtaat agcgattcgt gggaaatcag
     1441 gatcctccat cgttcgtcct tcacggatag gctgattggg aaactctttt attaaccaat
     1501 cagattcttt cgtcatctct ttgatggccc gataatactt tttggccata tcaatggaac
     1561 tcgtggttaa gatcgcagac ttcgttggac ggccatttcg aaaatcaaat tttgtatagg
     1621 cattgtctgg acggaacatt ttatgaatga ctttttggat atgttcctct gcttcaaaaa
     1681 cagtagggtc caaataactt tcttttttga cagactccat ttgatcgatc atacgattga
     1741 tctgttccgg tgaatactct gcatatgttt ctacttcttt taatttccga tagattttat
     1801 tctctaatga agtcggctcc acggtgtttt catgctctac ttgaaatcct aaaaccgagc
     1861 catcttctaa tgcattcttg atggtatagg tgtgcaagac ctctccatac tgatcatggg
     1921 tcgtacgtgc caattgccct ttggcttgtt tccgattctc agggaaaatc ggtgtacctg
     1981 taaagccaaa ccaagtggat ttcggaaaca tttttttgat ttcttccata ttttcagcac
     2041 ttaacgctcg atgacactca tcaactacaa acacgatatg ttgccccatt aatttttgga
     2101 agcgattggt tccttttttc tcttcctgtt tctttgcata tttcaacgcc gcatctagtt
     2161 tttgacgggt tgtaatgatc acaacattcg cattggcatc tgccaacaga gtttcactta
     2221 actctttggc acttcctgtt cccacgatca agctattcgc tttggcatta ccagaagaaa
     2281 ccccagtatt aaactccgaa gcaaatttcg taaactctgt ggtggtctga ttatctaaat
     2341 ctttgcggtc cactaacatg atcgttcgat cgatccccga ttttcgtgct aaaagcttag
     2401 tagatacaaa actagtcaac gtctttcctg aaccagtcgc atgccacaca taccctgatt
     2461 gatgtttatt tgcggcaata aacaaggctt gaatcgcatg cacttgataa ggatgtaaca
     2521 ccatgagggt tttattgtct tgatcttcac tgacaatcgt ataatccgca atcaagcgat
     2581 gggcatctgg gatatttagc acttgtttgc aaaactcata taagttttct acctttttat
     2641 tatctttcgt tcgccaacta aataaaaact taggatgcaa atccttcggt aaggcatttg
     2701 caaaataccg agtcgtttgt tcattggaaa ccacaaacaa ctgtaaggtc gaaaagatat
     2761 tgttccgaaa catgccttct tcagcatatt ttttgatttg attaaacgcc tgataaaaac
     2821 catcttttgc agttacttgt tttagctcga tttggataat cggtaagcca ttgatcaata
     2881 aggtgacgtc aaaacgtcga tcccgcgctt ctatgttact tccacgtttt gcaatttgat
     2941 ggaccacttc ataagtagag attcctccac caatgtcttg attggaataa aggataagtg
     3001 agacagaacc caattgcgga ttttcccgtt caatcgtgat ccgagccatc ccattttcac
     3061 cttttaacca tttcgcagca tcaaatgggg ttttcgtccg tagcaataat tcggttttga
     3121 tcgtttcaaa ctctttatcg gtcaaaggag aatcattcag ttctgcttga ttatttgaga
     3181 tgattttttg acgtaaattg ctccaaagat cttcttctga ttttagatcc ggacgatacg
     3241 tccactgatt atgtccttcc ccgaggacac gaatcaactg ttcttcgact tccgcttcct
     3301 cacggtgact aattttcatt cttcttccct ccctaaacaa acatcttttg caagaagcct
     3361 tttttcgttt ccttcagcaa atctaactta cgttgatgaa gagcgatcgt atcatctagc
     3421 tgcttgaaga aggcaccaat tttttgttgt tcatttatat ccggtggaaa ctgaaatgtc
     3481 ctttgttcta taccaccttt agtcacatgt accattgtcg ttccgttagt tgattcttta
     3541 attttctctt tatcagtcaa tagccaagta tatagatact gtttgtccaa ttccttagat
     3601 aaaaatttaa gtttccaaat atgataatgg tagattacac gttcttcatt ccacatttca
     3661 ggaccgaagc ttgttgccca taaatataag agatctcctt tattagcata cttgtcttcc
     3721 tctaattcta agtttgaata ataccatcta tcattagtat taaaattacc tactcttaaa
     3781 accctatatt ttccagaatc aagtaattct tgttgtttaa aagctctacc attcaaaaaa
     3841 actaccactt cccccaactt ccgctcttcc caatctcccg taaatcctgg aaaacgaact
     3901 tctggcactt ttgctccgtt tttagggaac attttttgta agaatccttt tttcgtttcc
     3961 ttcagcaaat ctaattcgtg ctggtgaagc atgatggtgt gatcaagttg tttgaaaaat
     4021 gaaccgatct tgatctgttc gtcataagtt ggaagtgaga taggtgtctt tttaccacta
     4081 tcaattgtat aggttccgac ggttccaatg cctgcaacgt cacggatata cttctgaatt
     4141 ttgttattta tgaacgagta gtaaaagaag tttccgtcaa ttttatgctc attcttgaac
     4201 caaatgatgt ttccatcctt aaagtaaatt gggttatcat cagtgattaa taaggggaca
     4261 cctattgatc cgacaccagt tacaagcaag tctccttgag aaactttacc tgaatttttc
     4321 gaatattcat tatatttttc ttctgaaatg taaagataat cggatggttc ctcatttttt
     4381 gcagctgaga caatatcacg agcacgtaaa aatctaatac ctgagttagt ccaatctgat
     4441 tgatgaattc gcttcacaga agtaacatcc atcatatctc ctaacttacg ctcttcccaa
     4501 tcttccgtaa aaccaggaaa ccggatttct ggtcgtgtat cattgctcat gtttgaacac
     4561 ctccagcgca ccttgtaacc acgcttcatt ttcaggtgtt gtttgcaggg aagagatagc
     4621 ttctaaaagt tctttttcta gcacttgctg ttcttgtcgt atcttcttga tgtcatttcc
     4681 gacatgaacc atatcaattg gttcttcttc ttcgaaagta tcgacatagc gtgggatatt
     4741 taagttatag tcattttctt tgatttcatc aaaggttgcc aaatgagcgt atttctcgac
     4801 atcttttctt tcagcgtagg tttcgaggat tttttgaatg ttttcttccg ataatttatt
     4861 ctgatttttc cctttaacaa attcacgact agcatcaatg aataatacat ctcgagtttg
     4921 acgattcttc ttcaatacaa tcaccgtcgt tggaatcgat gtaccaaaga acaagttcgc
     4981 aggcataccg atcacagcat aaatactgcc gtcttctaat aatttttgac gaatgacccc
     5041 ttctgcagca ccacggaata aaaccccgtg tggcaagacg atagccatag tccctgtttc
     5101 cttcaaatga tagaaaccat gaagaagaaa cgcaaaatct gctttggatt ttggcgctaa
     5161 tttcccgtag cgattaaacc gagaatcatc taagaaggtc gtatctgctg accaattagc
     5221 cgaataaggt ggattcatca ctactgcatc aaatgtgtaa ggttcatctg ttggccagtc
     5281 cttattcaac gtatccccat tgcggagatt catttcttcg gcatctaccc catgtaaaat
     5341 caagttcatt tttgctagat tgtatgtcgt tgtatttagt tcttgcccat gatatttaac
     5401 gttatctgga tgagttaagt agttacgtac atttaacatc aaggaacctg aacccatggt
     5461 tgggtcaaag acactaaaga atcgtcgttc tttttgatcc aacgtcacga tttgtgccat
     5521 catatcagaa accatatgag gcgtgtaaaa ttctccggct ttcttccctg cttctgaagc
     5581 aaattgactg atcagaaatt catacgcatc cccgatcaca tccccatcgt gacctaaaac
     5641 ttccacgtca ttcaatttct tgataacttc ggtaatcgtc acatttcgtt gttgctcatc
     5701 tgttcctagt ttttttgatt ggagatccac atcatcaaat aacccattaa attgattgta
     5761 ggtagatgcc aattgcacaa aggctttgtt tagatcgttc aattggaacg ttgcttgttt
     5821 tgcttgatcc gctaagacgt tgaaaagata ctctggtgag atcgaatacc ccaaa
//
LOCUS       DAXWOO010000159         5793 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.162, whole genome shotgun sequence.
ACCESSION   DAXWOO010000159 DAXWOO010000000
VERSION     DAXWOO010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.162"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(303..1085)
                     /locus_tag="ACGE70_002419"
                     /pseudo
     CDS             complement(303..1085)
                     /locus_tag="ACGE70_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1150..1986)
                     /locus_tag="ACGE70_002420"
     CDS             complement(1150..1986)
                     /locus_tag="ACGE70_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFF8694025.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(2004..3413)
                     /locus_tag="ACGE70_002421"
     CDS             complement(2004..3413)
                     /locus_tag="ACGE70_002421"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFF8694026.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3427..5250)
                     /locus_tag="ACGE70_002422"
     CDS             complement(3427..5250)
                     /locus_tag="ACGE70_002422"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFF8694027.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(5530..>5793)
                     /locus_tag="ACGE70_002423"
     CDS             complement(5530..>5793)
                     /locus_tag="ACGE70_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8694028.1"
                     /translation="AQIILLIPLYLGMIEIGYCLLIFTKKTSITIVAYIVGMLFIPSF
                     THQIHQFFPRAELLKLCDPLYAFLMLSRFWEFPAGYVGMVLLV"
BASE COUNT         1781 a         1259 c          947 g         1806 t
ORIGIN      
        1 gatacatatg aaacaattac atatgatttt ttcataaaaa aacagtactg tctagtagta
       61 taaaatatac aaatttctta aaaatttttt cactaaaatc actacctgaa acagtctaaa
      121 acacttatat atcaacgttt aatgaaacta tctccgtttc atttcagata caaataaata
      181 tagtcgggct ccaaatccga aaaaaaatac ccccagtagc ttatcctaag ctactggaga
      241 tgtataagta cggtcatata acaacggggt agggtaaata aatgaaaaag atggaagaat
      301 cattattcaa taactttttt aaatacttcg ttgtaatcag ttacctgtcc gttcttaaat
      361 accacagaat aatcttttta cccattttca agtaaccgaa gtggaatgtc tttaccaact
      421 tgctctgtca ctgcattact gatacctgtt agattagcta ataaatctga taaagattga
      481 gcatctgtag aaatctctgt atcagtattt atcgtgattc ctttatcttc atttacaagc
      541 ttccaagtag gtggtatagc tgtgtatgtt tcgatgaatg aatcaattct ttcatctatc
      601 gtcttcccag tatctacttc tgtctcatta ctcttagatg aatcgattct ttcatccatc
      661 gtctgctccg taactgcttc tgtctcatta ttctcaccgg aaactctctt cacacccaaa
      721 cttatatatt gtccatcttt agtgtgcata gttaaaattc ccacgctttt actaataaat
      781 tcactataac cagttctttc aatagagact tcaatgtatt tcacactatc atactcaaaa
      841 gaaaaataga catgtaaaag cattttgttg tttacatcta cataataatc ctttgactct
      901 acttgagacc ttcctatacg gaatacagct ttactgtctg atatctctat accagcatcc
      961 ttattattaa tctgccaatc tccaagtagc tcttctggca agctagtagt ttcttgtgaa
     1021 cactttacca cttcactggt agaggtcgaa acattcacct ccgcactgtt agaagaattt
     1081 tccaaggaat caggagcctt agcacaacca acaagtcttt atatattgtg cagccgatat
     1141 tttcttcatt cagtacaccc ttgaaatatg cagtgttaga taaagcagtt cgtcctgatt
     1201 tgctgaggca cccatctctt cttgaacgta ctccttgatt ttcaaggccg cccgatactc
     1261 tttcggaaaa cgctgcttca ctgaagccag caaaaaatca tccttctccg gcaactcttc
     1321 cttcttaatc attcgggcaa taaaaaactg taaatgcgtc acaaatctta agtaaagcat
     1381 cgaatcttta tcaacctttt tttcgggaaa cagatatcct agcatagcca agatatcctt
     1441 tagtgctctt accgagagca tggtttcttc catatttgct tgttcacttt gggcatttac
     1501 aatgtgaaac gcaatgtttc cagcctcctc ttctggcaac tgaacattca tttttttgtt
     1561 gatcaattgg atagcatagc agccgatctc aaattctttt gggtaatact tttgaatttc
     1621 aatcagcagg cgattttgaa aaacaatgcc tttttgactg cgttcaatgg catgcttcaa
     1681 gtgatcaacc aaggtaaaaa atagactttt attcagctta aagtcaaact tggcgttggc
     1741 ataatcgata attcgcttga cgcaaataat tagatcatcc ggagcagcct caaacagttt
     1801 aatgtaatcc ttttttagag catcagaaag tgaatctaat ttaaatactt tttggatttt
     1861 gtcagccgga accaaatcgc ctttctttag ttgaaatcca atccctttcc ccatcaaaat
     1921 tacttctttg ccttgcttat ctaagcaaag aattaaactg ttgtttaacg ttttaattac
     1981 tctcattaga ttcaccgttc tctctaagct aagccatttc ttgcgattac tttttgatac
     2041 caaaagaagc tttctttttt tattcgtttt aattctttaa gattctcatc ggttcggtca
     2101 acatatataa aaccataacg cttattgaag ccttgatggg tactaacaac atcaatcgct
     2161 gaccaaggta aataaccaaa aacatctaca ccatctgtta aagctgcttg aatagccaga
     2221 atatgtttgg ctagataatc aattcgatcc tggtcattaa ttcgtctatc ttcctcaact
     2281 atgtcgggtg ccccatagcc attttcagta attaaaatag gtaaatcata gcgatcgtac
     2341 aatttcctca atgttaatcg tagtccagta gggtcaacta cccagccata tggcgtttta
     2401 tctacataag gattttcagc tgcccggtag acaccttctt ctcccaacat gatctgttga
     2461 tcccccgctc tagcggaaac atctgatccg tcatttttgc tggcagcgat ggtggcagtt
     2521 gaataataat taatcgcaat aaaatcgggt ttaccggagg ataaaattgc ctgatcctct
     2581 gcctcaattt taggatataa tccacgatca ttcaaatagt tttcaaacaa ataattgtat
     2641 tttccccgaa ctgctacatc taagaaagac cagcctctca atgtttccca attatgggct
     2701 gcaattgcat cttctggact ggaggtagct tgatacatag ccgttaaatt gattgctgga
     2761 ccgatttttc ctttaagatt caactgatga tacaagtgaa ttactctagc ttgagctaat
     2821 aaaacatggt gattaatttg aaagagttct ttttttgaag gcagctctcc acttggtggc
     2881 aaaccaatcg ctccaggatg aaggatcatc gtgttttgct cattaattgt cagccaatag
     2941 ttcaccttgt caccatactc agtaagcaat acctgtacat agtccacaaa ataatcgata
     3001 gttgctcgat ttaaccaacc gccctgctcc tctagaaaat aaggtaaatc gaaatggtac
     3061 atggtgacaa tcggttcgat tccctgattc aggcattcat caattacttg atgataaaaa
     3121 gaaattccct tttgattggt tttattattt tcaatatcca taattcgcgt ccaagcgatg
     3181 gaaaagcgaa aagcttttaa gcctaattct ttaaacagct gtatatcttc tttatagtga
     3241 tggtaaaaat ccgaggcaac ttcaaaatta gctacccctt ctgtatgctg atacttgtca
     3301 ataatagacg gttgtttccc atcctcctga taggcaccct ctgcctggta ggcactaatg
     3361 gaggcacccc ataaaaactt ctctggaaat gctgaaagct gtttgtattg cacaaaaatc
     3421 ctctccttaa ctaatttgca tcaatggttc gtgttccttt aagcttccgc ttgcttcact
     3481 aattcctgcg taattttcac tgttggtcac taccacaatc actgtatcat cgtagccagc
     3541 gtttctaatg gcagcgaaat cggcgttgat taataattgt cctgctgaca cctgatctcc
     3601 tgcttttaca aatacttcaa agggttcccc ttttaaattc acggtgtcga tccccacatg
     3661 gatcaataat tcaatacctt ctttagaacg tagaccaatc gcatgtttag attctaaaat
     3721 agtaatgact tctccctcaa caggtgaata aatgctgttt tcttctggat gaatggctac
     3781 gccctttccc aatagtccct tagaaaagac agcatcgttc acttcagaaa taggacacac
     3841 ggttcctttt accggatttt caacaatgac tgcttcggct attgccgttt gctctgattc
     3901 atcagaagta tcgtcaaatc caattagata agcaatgata aaggttgcaa caaaggcgat
     3961 tacacacgta ataattgcgt gaacaatatt cattggattt tctccaataa aggctggtaa
     4021 ggctgctaag ccaggggaaa caaaggcata tctgactaaa ccgctcaagc cagcatacaa
     4081 accagcaatt gcaccaccaa tcatggaagc aactaatggt tttttcagct tcaaggttac
     4141 tccatagagt gaaggctcag taattcccat taaagcagtg atcccagtgg acacagctag
     4201 ctgcttcaac tctttgtttt tagttttcgc ggctacagca aaggtagcac ctgattgagc
     4261 aatattagaa gccagcattc ctggcccatt gatcatttca gaacccgtac tgctcaattg
     4321 catagtagcg attggcgtca ttgcccaagc tgtacccgta accaccaaaa atggctgcaa
     4381 accacccatc aaaaatggaa ttaaccaagc tgctttgccg ttaatccatt cagcccccat
     4441 tgctataaga tcgttcagca acgttccaaa cggtccaatg acaaccaacg ctaaaggccc
     4501 cataattaaa aataccagca tcggcttagt aaagaattta ataaacgaag gcgaaattcg
     4561 atccgcaaag ggctctacat aggacatgat ccaaacagta aagataatcg gtaaaactga
     4621 tcccgcataa tctgcagcta ataccggaat tcctagaaaa gaaacggcat cgccagtaga
     4681 aaaaagctgt gaaatatttg gatgaagcaa aatgccagca acagtaatcg ccaaaattgg
     4741 gttgacatca aactttatgg ctgccccata ggctaatagt actggcataa aataaaaggc
     4801 tgcatcagca atagtattca aaatgatgta agtttgacta ctgtctgata gtacaccagt
     4861 caaagtcaag acggccaaaa tggcttttac catcccggca cctgttatag ctggaatcat
     4921 cggtgtaaag gtggtagaaa tgacactaat caatcttgaa ataatacttt cttgattgtc
     4981 tgaagagggg ccctttttaa ttgaagttcc cacttccttt tgaatttcct tgaaggctgt
     5041 ttggacttca ttgccaatca ccacttggta ttgtccaccg ctatccacca cactaattac
     5101 accaagtgtt ttttctaagg ctttcgtatc ggctttcctt ttatctttga gttcaaaacg
     5161 cagtctggtg gcacaatggg ttaagctgac cacattttct tttcccccaa cctgttgcaa
     5221 tacatttctt cctacttctc tgtaatccat tattctctct ccttaaagca gtaaaaaaga
     5281 ccaagtcaaa ccacgcccca atcaacattg aagtgttagt ttgtcttggt cttgcctgca
     5341 taatcagtaa catgccaata acgttatttt gctgtaatcc aataatagca cattatgata
     5401 gcgcaatcaa cagggaattt aattttttat cttttcgttt gaccgtatag ggtaatataa
     5461 cagttagatc acatcaacta gggtatatct gcgtatactt actgctaaaa taagtatatc
     5521 taatattaac tataccagta aaaccattcc tacataacca gcgggaaatt cccaaaatcg
     5581 tgaaagcatc aaaaatgcat ataacggatc acacaacttc aacaattctg ctcttgggaa
     5641 aaattgatga atttggtgag taaaggatgg aataaacagc attccaacaa tataggcaac
     5701 aatagtgata cttgtttttt tggtaaaaat cagtaagcaa tagccaattt caatcattcc
     5761 gagatataaa ggtatcaata gaatgatttg ggc
//
LOCUS       DAXWOO010000160         5792 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.163, whole genome shotgun sequence.
ACCESSION   DAXWOO010000160 DAXWOO010000000
VERSION     DAXWOO010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.163"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            25..882
                     /locus_tag="ACGE70_002424"
     CDS             25..882
                     /locus_tag="ACGE70_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFF8694029.1"
                     /translation="MEEKLIKKDEIIMGLLVPRANTYTIPLTKRQLIAKRYQRITRAI
                     NREFWNSESDTAHSLYVGSYGRGTAISTSDIDIIVELPMAEFDRFKNYLSNGPSKLLQ
                     VIKNAFQEILPNSDIRADGQVVKINFHDGIKFEIVPAFNEKDYWGESKGFIYPDSNMG
                     GNWKATNPKKEQEAMKLKNTKSNNLLYATCKHFRHVRDTEFTSYHLSGIVIDSFVYEA
                     MGNWKFVENNSGGQNISSVSYETALLEYYNSHKVMGGLNLYSPGSNQFVNSDSSIICL
                     EKVLKKIAL"
     gene            902..1462
                     /locus_tag="ACGE70_002425"
     CDS             902..1462
                     /locus_tag="ACGE70_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HFF8694030.1"
                     /translation="MGDEKKRLEVQLREAYGRVTYTYTTHLKQADNIISDNKILRCLQ
                     IGLSALTTGGVLSTIITDNSELTFVAGLCSTISLGLNLYFKNFDLAEEAKNHQIVADK
                     LWLIREEYVSLLTDLNRLSVEDIVKKRDQLMNETHEAYKNAPKTNSKSYESAQKALKS
                     EEEQFFTEEELDKMLPEHLRKKSSKY"
     gene            2351..3118
                     /locus_tag="ACGE70_002426"
     CDS             2351..3118
                     /locus_tag="ACGE70_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HFF8694031.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRI
                     GRSKPRAFILIKEKYLNEWNSTYEMVMTDNFEKVLDFRNALLGSDMGNSSLESIEVEN
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYEFPSENDKSF
                     TTNLKNAGKTIGIELLDHIIVTSKNTSYYSFQENGSL"
     gene            3144..3689
                     /locus_tag="ACGE70_002427"
     CDS             3144..3689
                     /locus_tag="ACGE70_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694032.1"
                     /translation="MGERTQALITVKYGNSKTKASVSIHYQWGYGRYMLMDVLNQGIY
                     LINKAYDLRDKSREELAKGIFDRSTGVINFVTYESGVSAYDETGFGGRYEPYQETIED
                     VMKFSDNNDGYVHLEMVFDENGFSLDDKTRLRLFDFEGQQIGLNKFCESGKEFATKDF
                     IKGYRLLLENYGINLEEESVK"
     gene            3690..4256
                     /locus_tag="ACGE70_002428"
     CDS             3690..4256
                     /locus_tag="ACGE70_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694033.1"
                     /translation="MFEKRKDQFPNLEAKSQKVIDGEMIELFQKSYGTIETTASCKYY
                     FQGKRNTFKGFADSDEELAKKIIDWLVSSEHVAREDTVRTTINGEVLTGTRCVCCNHE
                     FVEDEGCYRINEYGDFICQSCHEIEFPTERSWFEYMLQDYEMEKENGEVVFCTKEELS
                     KRSDDEIEDWCVWLVGADDCVAYYTTAE"
     gene            4271..4519
                     /locus_tag="ACGE70_002429"
     CDS             4271..4519
                     /locus_tag="ACGE70_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694034.1"
                     /translation="MEGMDFLDHEDLVDFGYTWKGMVGISRSLANGFYERNYAVYVLY
                     DDDTESLVDEEYKLDLENVLYGIEKEDLAKYIFSWLGQ"
     gene            4539..4778
                     /locus_tag="ACGE70_002430"
     CDS             4539..4778
                     /locus_tag="ACGE70_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694035.1"
                     /translation="MTKFKSKGMQWCSYYYVGNDEDLLRKTQRKGYPDKILKQIGYYM
                     PEERIEKEFCEVLFDSEQLLVLLTQNKLICQFENN"
     gene            5149..5430
                     /locus_tag="ACGE70_002431"
     CDS             5149..5430
                     /locus_tag="ACGE70_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFF8694036.1"
                     /translation="MKVKNPTEKSRIQVGIDKNLKENAEMILEELGLNPTTAITILYK
                     QVVARGEFPVEIKLSEEEKQGIRLQQLTKDMPVDVLDTDEKLEEWFDEA"
     gene            5420..5761
                     /locus_tag="ACGE70_002432"
     CDS             5420..5761
                     /locus_tag="ACGE70_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFF8694037.1"
                     /translation="MKPNEIVTVYVAFTDKNGGKRRPILVVSDKEDRVEFFGITSQYE
                     KKSDQIKRVYFPIAEWEKTGLKKQSWIDVGSLKAIPKSRENISFKKVGMLSASDTKKL
                     NRFIEKLQRKV"
BASE COUNT         2161 a          780 c         1167 g         1684 t
ORIGIN      
        1 tattatattt agatatcata atttatggaa gaaaaattaa ttaaaaagga tgagattata
       61 atggggttac tggttccgag agctaatact tatactatac ctttaacgaa acgccaatta
      121 attgctaaaa gatatcaaag gattactaga gccatcaata gagagttttg gaattctgaa
      181 agtgatactg ctcacagttt atacgtaggt tcttatggga gaggaacggc aattagtact
      241 agtgatatag atataatagt tgaattaccg atggcagaat ttgacagatt caaaaattat
      301 ttatcaaatg gcccgtctaa gttattacaa gttataaaaa acgcatttca ggaaatacta
      361 ccaaatagtg atattagagc tgatggacaa gttgttaaaa taaatttcca tgatggaatc
      421 aaatttgaaa tagttccagc ttttaatgaa aaagattatt ggggtgagtc aaaagggttt
      481 atttatccag attcaaacat gggagggaac tggaaagcta caaatcccaa aaaagagcaa
      541 gaagccatga aattaaagaa tacaaaaagt aataatttat tatatgctac gtgtaaacat
      601 tttcgacatg tacgagatac agaatttacg agttatcatc tttcaggaat agtaattgac
      661 agtttcgtat atgaggctat gggaaattgg aaattcgttg aaaataacag tgggggtcaa
      721 aacattagtt ctgtatctta tgagacagct ttattagaat actataactc acataaagtt
      781 atgggcggtt taaatttata ctctcctgga agcaatcaat ttgttaattc tgatagtagt
      841 atcatatgtt tagaaaaagt attaaaaaaa atagctttat aaagttttat ggaggaacaa
      901 catgggagac gaaaaaaagc gcttggaagt tcaattacgt gaagcatatg gacgagtaac
      961 gtatacttac accactcatt taaagcaagc cgataatatt atttctgaca ataaaatttt
     1021 aaggtgtctt caaattggtt tgtcagccct tactactgga ggggtactta gtaccattat
     1081 tactgataat agtgagttaa cctttgttgc tggattatgt tcaacaattt ctcttggatt
     1141 aaacttatac tttaagaatt ttgatcttgc tgaagaagct aaaaatcatc aaattgttgc
     1201 cgataaatta tggttgatca gagaagaata cgtaagctta ctaacagatt taaatcgttt
     1261 aagtgttgaa gatattgtga aaaaaagaga tcaattaatg aacgaaactc atgaagcata
     1321 taaaaatgcc cctaaaacaa atagtaaaag ctatgaatca gctcagaaag cattaaaatc
     1381 tgaagaagag caatttttta cagaagagga attggataag atgttaccag agcatcttag
     1441 aaaaaagagt tctaaatatt gaactctttt tttatgctta aaattctctt gtaatttttc
     1501 tattcatcta tatttaagaa gaaatatgat gatagaaaca tctcctaaag ccaccgcaag
     1561 ccgttctaag catagcttag cccgacattg ctagatatgc tcatctcacc atgaacgttt
     1621 cctaaagcca ccgcaagccg tcctagccat tgcctagtcc gacatcgcta gattagtgca
     1681 tctcgccata aaagttttct aaagccaccg caagccgttc taagcatagc ttagcccgac
     1741 attgctagat atgctcatct caccatgaac gtttcctaaa gccaccgcaa accgtcctag
     1801 ccatcgccta gtccgatttt gcttgatcgg ttccatttct gtttatttag tgggtcaaca
     1861 ccgtaatctt aaatagccat cgcctattta agattactag gtctgctttg aaggtaaaaa
     1921 gtctttcatt cttcaagact tttaggcttt caagctacgc ctgaaagcat tgaaattcat
     1981 cgctctttat ttggttttgc catttataat tccttacgcc cattatcttt ttcgcctgtc
     2041 ataccttgtc agaagtgttt agagcttatc tcatctttgg attaatacaa gtggcgtaaa
     2101 tttttttcta aacgcaagcg ttacgaaaat aatttactta aacacttgta ttaatccggc
     2161 agataagaac gcattctaat cactcccgac aaggcaagac aggcgaaaaa gataaataaa
     2221 tgaacgaaag gaagaaacat aaaatgcaaa accaaatagc gatgaagaaa aaaccacctc
     2281 cctctaaaac aatccaaaca aaaaagaatt ttgaaaaacc aaaaatcaaa aacagaaaga
     2341 agcgttcaac atgagagata gaattttaga agttaatcaa gaaatggtaa acaagttaac
     2401 aaatatcagt gggaactatg aaaagtacga tggtacttta cttgataact atatttttca
     2461 cgatacacaa aatatcagaa ttggcagaag taaaccgaga gcgttcattc tcatcaaaga
     2521 gaaatatttg aatgaatgga atagcaccta tgaaatggtt atgactgata actttgaaaa
     2581 ggtattggat ttcagaaatg ctttgttggg ttctgatatg ggaaatagca gtcttgaatc
     2641 tattgaagtt gaaaacaaga ccgagaatga aggtgaagga ttgattaaga atgtcttgaa
     2701 aatcgaacaa aaatatatca gtgaagatag aagtttttat agagtcacaa gctcggttgg
     2761 tctagcaaac tttgctacta acttaattgg aaacaatgct agtgaatctt tgctagtgat
     2821 gtgtctgaat acaaagaatg aggtcattgc ttatagcgaa gttttcaaag gtggaattaa
     2881 tcaatcagta gcgataccaa gagagatttt tcaactagct ttgctaaaca attcagcaaa
     2941 aatcgcagta tcacataatc atccgagcgg atatgaattt ccaagtgaaa atgacaaaag
     3001 ttttactacg aatttgaaaa acgcaggaaa aacaattggt attgaattgt tagaccatat
     3061 catcgtaaca agtaaaaaca caagctatta ctcatttcaa gaaaatggtt ctttataaaa
     3121 attagagaaa agaggaaaac aagatgggcg aaagaacaca agcgttaatt acagtcaaat
     3181 atggtaacag caaaacgaaa gcaagcgtgt cgattcatta tcagtggggg tatggtcgat
     3241 atatgctgat ggacgtattg aatcaaggaa tttatttaat caataaagca tacgacttac
     3301 gagataaatc aagagaagaa ttagcaaaag gaatctttga tagaagtaca ggagttatca
     3361 attttgttac ctatgaatct ggagtgagtg cttatgatga aacagggttt ggtgggcgct
     3421 atgaacctta tcaagaaacg attgaagatg tgatgaagtt ttcagataac aatgatggct
     3481 atgttcattt agagatggtg ttcgatgaaa atggatttag tctagatgat aaaaccagat
     3541 tgcgattgtt tgattttgag ggtcaacaga taggactaaa caagttttgt gaaagcggaa
     3601 aagagtttgc tacgaaagat tttattaagg gatacagatt attgctagaa aattatggaa
     3661 taaacctaga agaagagagc gtgaaataag tgtttgaaaa aagaaaagac cagttcccta
     3721 atttagaagc gaaaagtcaa aaagtgattg atggtgaaat gattgaatta ttccaaaaaa
     3781 gttatggaac catagaaact accgcaagtt gcaaatacta ctttcaagga aagagaaaca
     3841 cgtttaaagg ctttgccgat tctgatgaag aattggcaaa gaaaatcatt gattggcttg
     3901 tcagcagtga acacgtagca agggaagata ccgttagaac aacgattaat ggggaagtcc
     3961 taacaggtac tagatgtgtt tgttgtaatc atgaatttgt agaagatgaa ggttgctacc
     4021 gaatcaatga atatggtgat tttatttgcc agagttgcca cgagatagaa tttccaaccg
     4081 aaagaagttg gtttgaatac atgctacaag attatgagat ggaaaaagaa aatggtgaag
     4141 tagttttttg taccaaagag gaattgagta agagaagcga tgatgaaatt gaagattggt
     4201 gtgtgtggtt agtaggggca gatgattgcg ttgcatatta cacaacagca gaataggagt
     4261 gtgaatcagc atggaaggaa tggattttct agatcatgaa gatttggttg attttggtta
     4321 tacatggaaa ggaatggtag gtattagcag aagtcttgct aatggctttt acgaaagaaa
     4381 ttatgcagta tatgttcttt atgatgacga tacagaaagc cttgttgacg aggaatataa
     4441 attggattta gagaatgttt tatatggaat agaaaaggaa gatttagcaa aatatatttt
     4501 ttcatggttg ggtcaataag tgaaaggaag atagaaacat gacaaaattt aaaagtaagg
     4561 gtatgcaatg gtgttcttat tattatgttg gaaatgatga agatttattg agaaaaaccc
     4621 aacgaaaagg ttatccagat aagatattga aacaaatagg ttattacatg ccagaagaga
     4681 ggatagaaaa agaattttgt gaggttcttt ttgatagtga acagctatta gttttgttaa
     4741 cccaaaataa attgatttgc caatttgaaa ataattaaga tgaggtggtg aaccatgaat
     4801 gaagataaga tggtattaag tgaatatgct agattgagaa ctattaattc tttagcatct
     4861 catttaagtt gttctttcct aacaaaggtg ctaaaattaa tttacaaatg tgaagatttt
     4921 gtacaagaga agcacaaaga caagcctact gcgaataggc ttgtcttttt ttcattcatt
     4981 acgattccgt atgtgtgggt tagcgacaat ttgttcaatt accgtatgat atagcatctt
     5041 aatttgatta gattacttgc aatgtagttt attaagagta taatataatc ataaatgtgg
     5101 acaatttgat aaataatgag tgttttatac tcaaaaggag tggatgaaat gaaggtcaaa
     5161 aatccaactg aaaaaagcag aatccaagtc ggaatagaca agaatttaaa agagaacgca
     5221 gaaatgatat tggaagagct aggtttaaat cctacaacag caataacaat tctttacaaa
     5281 caagtagtcg ctagaggtga gtttccagta gagataaagc taagtgaaga ggagaagcaa
     5341 ggaatcagat tgcaacagct aactaaggac atgccagtag atgttctaga tacagatgaa
     5401 aagttagaag agtggttcga tgaagcctaa tgagatagta acggtatatg ttgcatttac
     5461 agataaaaat ggtggtaaaa gaagacctat tttggtggtt agtgataaag aagatagagt
     5521 agaattcttt ggaatcacaa gtcaatatga gaaaaaatca gaccaaataa agagggtgta
     5581 ttttcctatt gcagaatggg aaaaaacagg acttaaaaag caatcatgga ttgacgtagg
     5641 gtctttaaag gctattccca aaagccgaga gaacatatca ttcaaaaagg ttggtatgtt
     5701 atcagctagt gatacgaaaa agctaaatag gtttatcgag aaattacaaa gaaaagtcta
     5761 acaaggcttt tcttttttgt ttatatgtat at
//
LOCUS       DAXWOO010000161         5723 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.164, whole genome shotgun sequence.
ACCESSION   DAXWOO010000161 DAXWOO010000000
VERSION     DAXWOO010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5723
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.164"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(<1..268)
                     /locus_tag="ACGE70_002433"
     CDS             complement(<1..268)
                     /locus_tag="ACGE70_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HFF8694038.1"
                     /translation="MRTHYIYLEDKIVDEIPAKSREQARKLFIEKYKEINGDSEVPKV
                     VVTVRKRQTKKRSTFHVSPDIYNLIERHRSEDNISISQYIATVID"
     gene            complement(523..843)
                     /locus_tag="ACGE70_002434"
     CDS             complement(523..843)
                     /locus_tag="ACGE70_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694039.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEIFFIEEWEGTENLVLHQY
                     LLKA"
     gene            complement(876..1964)
                     /locus_tag="ACGE70_002435"
     CDS             complement(876..1964)
                     /locus_tag="ACGE70_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694040.1"
                     /translation="MQNKIKRLVGLGWMTRGKLDSFSTIKYLLMDIYRQNKQTGIHFL
                     GYDKPEDAPFFVPSSAYRASTSGSMYNLDLFEEGAKMIEYDFNEAYSRIMRNYPLPSN
                     TYLKAKYNTDQAILRAINRPKVRPYENLKTYMFFLVNFEGTAKEGTYANPKSQSMFAD
                     YGENPKTANEPIWLSEIELTLILDYYDIKEMEVLDSHTFLTKKGLLKEYFERIEPLKT
                     DPDTLKLYKNLRTKVFGAIGQKSLREGETSTFDKYSMYNRAYSSMVAGVFRDRMVRYE
                     QKYVHSAYGLLKIRTDGLYFRSEVPEFEELYKLGVVKKKVHTISKEEVEKALEFKNKG
                     FENKGSFGKSKDEVKGVVIDDDFEFDFD"
     gene            complement(2023..2817)
                     /locus_tag="ACGE70_002436"
     CDS             complement(2023..2817)
                     /locus_tag="ACGE70_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694041.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSRRLRITVEYGTNESATELKQRYLDTRREASKKYDDFKSRGLIPK
                     KKKGIWREFFKEEDSNRNKGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNTL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLALAKKARDLRETQ
                     MYLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMNKYRV"
     gene            complement(2827..3702)
                     /locus_tag="ACGE70_002437"
     CDS             complement(2827..3702)
                     /locus_tag="ACGE70_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694042.1"
                     /translation="MTTNKRWSELVQNTVKKVMPDGYHFNYIQCSQTTESIYFSILNE
                     NRLYYFRVSSHHRKKREDNVTTFLSGKYNGFKELSEAIGEYLKSNRFVRISDKHILIL
                     LLYLNNSFKLFWRDDTFYYIENGMDIYSGINGILKHYIKALLRYGVLYQEDLSNQIKI
                     SNSGKSLLAKYKKLLSPIYKGIDMEAVNSETLFNLVVDEKFANEFFKSKKIERLNNLF
                     PENAIKAYVDNNKSRLYNWFIPKRLRGKIKIGDQVKVINLNHTKIVTVTELYIAEKED
                     ITKMKPVIKILDNKE"
     gene            complement(3919..4176)
                     /locus_tag="ACGE70_002438"
     CDS             complement(3919..4176)
                     /locus_tag="ACGE70_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694043.1"
                     /translation="MINEDFRSENKMIDISLIEKLLFSNKYTAKELATRWQISESAIK
                     AYRQNKGSVGWRDWKGISIGKAEDIMKREGFQADNDSKKVE"
     gene            complement(4179..4403)
                     /locus_tag="ACGE70_002439"
     CDS             complement(4179..4403)
                     /locus_tag="ACGE70_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694044.1"
                     /translation="MNQRRRKRLEEISERLSELKDELEVIQEEEYKENIPKNLQGSER
                     YEKAEESVDQLEEAVDGLSDILELIEDIAS"
     gene            complement(4576..5619)
                     /locus_tag="ACGE70_002440"
     CDS             complement(4576..5619)
                     /locus_tag="ACGE70_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFF8694045.1"
                     /translation="MKKFLVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGTPVYRLYNRYNGEHFYTVDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNVISKQGWKIEGTGWYAMPNS
                     VPNPTPAPSTEKVLNVPVVWQGNTMLCEGASLLEGLHYKGVATNQSLTSFVNYMPKAN
                     DNNPYHGYSGEWRHNVNGTYQGMMADPVVKWAKNVGGNAVNITGSGTNGIKNEIRNGN
                     PVVTWITYNYATPEFKQMPWGRAVWNGHVVLVDGFRNGAYHVVDPVFGAKWINASTYE
                     RAFNVTGMAVAVR"
BASE COUNT         1580 a         1214 c          737 g         2192 t
ORIGIN      
        1 catcaataac tgtagcaata tattgagaga tagaaatatt atcttcactt ctatgtcgct
       61 ctataagatt atatatatca gggctgacat gaaacgtact tctctttttt gtttgccttt
      121 ttctaactgt tacgaccact tttggaactt cactatctcc attgatctct ttgtattttt
      181 caataaataa ttttctagct tgttctcgac tttttgccgg aatttcatct acaattttat
      241 cttctaaata aatatagtgt gttctcatac attcgtcctc tatcttcttt actctcaatc
      301 ttcatgatct tagttcttac cttgtttact cttaaacatt gcaaaaaaat ctagtcaaaa
      361 tcaaacttga aatcatcgtc tatcacaaca cccttaactt catctttaga tttcccaaag
      421 gaacccttat tttcaaatcc tttatttttg aatgatgatg gttatggaaa acttcctttg
      481 aaattctgac aagcataact agtcagaact gttttactta gattaagctt tcaaaagata
      541 ctgatgaaga actaagttct cagtaccttc ccattcttca ataaaaaata tttctccttc
      601 ttttacccac tctatagcta atttcttata atcaaataaa ccatatgtgt ttaagccttt
      661 gtctttcatt aaactcaaaa actgatcccc ttttaatctt tctgtatcat ccatcagaaa
      721 ttcaactaaa tctttatcca tgcgtgcaac tttttcgtct tttagtgcca ttgatttagc
      781 atagtcagtt gaatctttta gaataataac acctacattt ccttccacaa ttttcttctt
      841 catatttacc tcctaccatc ttttccaaag agtatttagt caaaatcaaa ctcgaaatca
      901 tcgtctatca caacaccctt aacttcatct ttagatttcc caaaggagcc cttattttca
      961 aatcctttgt ttttaaattc taaagctttt tcgacttctt ctttggatat tgtatgaact
     1021 tttttcttaa ctactccaag tttatacagc tcttcaaact ctggtacttc ggatctgaaa
     1081 tacaatccat cagtacgaat ttttaatagc ccatatgcgc tatgaacgta tttctgttca
     1141 tatctaacca tacgatctct aaatacgcct gctaccatgg aggaatacgc tcgattatac
     1201 atactgtatt tatcgaaggt gcttgtttct ccttctctta atgatttttg tccgatagca
     1261 ccaaatactt tcgttctaag atttttataa agcttcaacg tatcaggatc agtttttaat
     1321 ggctcgattc tttcaaaata ttctttgagt aatccttttt tcgtcaaaaa tgtgtggcta
     1381 tctaggactt ccatctcttt aatatcataa taatcaagga taagtgttag ctcaatctcc
     1441 gataaccaaa taggctcatt agctgttttg ggattttcgc cataatccgc aaacatcgac
     1501 tgtgattttg gattagcata tgttccttct ttggctgttc cttcaaaatt aaccaaaaag
     1561 aacatatacg tttttaaatt ttcatacggt cttacttttg gtctatttat cgctctcaaa
     1621 atagcttgat ctgtattata ttttgctttc aaataagtgt ttgacggcaa aggataattt
     1681 ctcatgatcc tcgaataggc ttcgttgaaa tcatattcga tcatctttgc tccttcctca
     1741 aacaaatcca agttatacat gctaccggag gtactggctc tataggctga acttggaaca
     1801 aagaaaggtg catcttccgg cttatcatag cccaaaaaat gtattcctgt ttgcttgttc
     1861 tgacggtaaa tgtccatcaa aagatatttg atcgtactaa aagaatctaa ttttcctctt
     1921 gtcatccacc ctaatccgac aagtctttta attttattct gcatttcagg tgtgtatatg
     1981 ctattgtttc ccatttatct gctcatctcc ggctttttta ttctagaccc tatatttgtt
     2041 cattacacca cttatctcgt ctacaaaggc tcccagatcg tgtgtaaagg catcatagta
     2101 atcttcaatc tcactcgatt cagttctccg gtttcttccg tttttatttc tgaatagata
     2161 catctgcgtt tctcttaaat cccttgcttt tttcgcaagt gctaataaat ccgtaaaatt
     2221 atcaataacc ataatgtcat tgatcataaa accttcttca tgaccatact tttcagccat
     2281 ctcgataaaa tcattaacat cgaacaactc caataattct gctttatatt tctctaacaa
     2341 tgtatttaaa tagcgatttc tcaattccca tgaactttca ccagttccgt attcaacctt
     2401 gatccctaaa ttcttagtgt agccttttgc tgtgtatcgt ccaatgaagt ctcccttatt
     2461 ccgattgcta tcttcttctt tgaaaaactc acgccaaatc ccctttttct tcttaggaat
     2521 tagacctctg ctcttgaaat catcatattt ttttgaagct tctcttcgtg tatctaagta
     2581 cctttgcttc agctctgtag ctgattcatt cgttccatat tcaacagtga tccttaagcg
     2641 cctgctataa ccttttgctg tatatcgtcc aacaaagtca ccgccacttc gatcactact
     2701 gacttcctta acaaattctc tccatatacc ttttttgtcc tttggtatta atcctcgtct
     2761 tttcatatct tgatattttt cagaagcttc ttttagagta ttcttttttg ctaccatata
     2821 ttccactcac tctttgttat ctagtatttt aataactggt ttcatttttg taatatcttc
     2881 tttttcagct atatacaact ccgttacggt aacgatctta gtatgattta gattaattac
     2941 tttcacttga tcgccaattt tgatttttcc tcgcaaccgt ttgggaataa accagttgta
     3001 taatcgtgat ttattattat ctacataagc ttttattgca ttttcaggaa acaggttatt
     3061 tagtctttca atttttttac ttttaaaaaa ttcattcgca aacttttcat caacaactaa
     3121 attaaataaa gtttcagaat ttacagcttc catatctatt cctttgtaga taggagaaag
     3181 caatttttta tattttgcca gtaatgactt tccagaatta ctaatcttaa tttggttact
     3241 taaatcttct tgataaagaa ccccatatcg aagtaacgcc ttaatataat gttttaatat
     3301 gccgttgata ccagaataaa tatccatgcc gttctcaata tagtagaaag tgtcgtctct
     3361 ccaaaacaat ttgaagctat tattcaaata aagtaacaag atcaatatat gtttgtcaga
     3421 aattctgaca aatctattac tttttagata ctcaccaatg gcttcactta actctttgaa
     3481 gccattgtat tttcccgata aaaatgtcgt aacattatct tctctctttt tcctatggtg
     3541 tgaacttacc ctgaaataat agagtctatt ctcatttaat attgaaaaat aaatactttc
     3601 agttgtttgt gagcattgta tatagttgaa atgatatcca tctggcatga cttttttaac
     3661 tgtattttgt actagctctg accacctttt gttagtagtc atatattttt ttccattaaa
     3721 ccaaacataa atatcttttt taaaaatttt gttccccccc tattgcgttt tctttgacat
     3781 taaagccctt tgttttcatc acattcgtat agccctagcc actcataacc tgatgctttt
     3841 gcgaagttta tttaaaagtg ttttcctttg taatctacct ttcccctcgt cctggtactt
     3901 ttcttggcga ctattaaact attcaacttt tttagaatca ttgtcagctt gaaatccttc
     3961 acgtttcatt atgtcttctg cttttccaat gctgattcct ttccaatcac gccatcctac
     4021 gcttccctta ttttgacgat aggctttaat agctgattcg gatatttgcc atcttgtagc
     4081 caactctttg gctgtgtact tatttgaaaa aagtagcttc tctatcagtg aaatatcaat
     4141 cattttattc tcactcctga aatcttcatt aatcatctct agctagctat atcttcaatc
     4201 aattctaata tgtcggataa accatcaaca gcttcttcaa gttgatcaac ggattcttct
     4261 gctttttcat atctttctga tccctgtaga tttttaggta tattttcttt gtactcttct
     4321 tcttgaatta cttctaactc atcctttagc tcggaaagtc gctcgcttat ttcttctaac
     4381 ctttttcttc ttctttggtt catcaaaatc acctcttcgc ataatctcta cgattttaat
     4441 ataccacttt tgtgtactac attcaatatt ttttgttata gattcgtatt acaaaaattt
     4501 cctctaaaaa aataagagcc tatttaccat ttcagtaatt aagctcttat ttcatttagt
     4561 tgataattct atcaattaac gtacagcaac cgccattcca gtcacattaa atgctctctc
     4621 atacgtgctt gcattgatcc attttgcgcc aaatacaggg tcaacaacat ggtatgcacc
     4681 atttctgaag ccatccacga gaactacatg tccattccat actgcacgac cccaaggcat
     4741 ttgtttgaac tctggcgtag catagttata agtaatccaa gttacaaccg gattaccatt
     4801 gcgaatttca ttcttgattc cattcgttcc tgaaccagtg atattgacgg cattaccgcc
     4861 aacgtttttt gcccatttaa ctactgggtc agccatcatg ccttgatacg ttccattaac
     4921 gttatgacgc cactctcctg agtaaccatg ataaggatta ttgtcattag cttttggcat
     4981 atagtttaca aaactggtta agctttgatt tgtggctaca cctttgtagt gtaaaccttc
     5041 taataaagaa gctccttcgc ataacattgt atttccttgc catacaacag gaacgttcaa
     5101 cactttttca gtgcttggag caggtgtagg atttggtacg ctgtttggca ttgcatacca
     5161 gcctgtacct tcaatcttcc aaccttgttt actaattacg ttcttttcat tttgatctaa
     5221 cgtgtagtga tgccaattca cgtatttgtt atataaacgt gttacaggaa ctctatttgc
     5281 tccgttgtag gaatagaacg caacaccttc gtacgtccat cctgtgtgtg aaatcttatc
     5341 tctttctccg gcatcaactg tgtaaaaatg ttcaccgtta taccgattat acaaacgata
     5401 aacaggagta ccacttgttg gagcttgcca tgcgacacct tcgtaatgcc atccataacg
     5461 tttcagattg tctctttctc caacgcttct agtgtataga tgctctccgc tattgggatt
     5521 atacatacga tacatggaaa tagagctttc tgctgaaact gaaggtgata ataatactag
     5581 ccccattgtg aatagaattc ctactaaaaa ttttttcatt tgattttacc ccttttcatt
     5641 ttttattgtc attattgcat gaaaattatt cgtaagattg ccaattacat aaacgattgt
     5701 tttaagatca taatcgttgt tgt
//
LOCUS       DAXWOO010000162         5705 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.165, whole genome shotgun sequence.
ACCESSION   DAXWOO010000162 DAXWOO010000000
VERSION     DAXWOO010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5705
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.165"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            329..1252
                     /gene="truB"
                     /locus_tag="ACGE70_002441"
     CDS             329..1252
                     /gene="truB"
                     /locus_tag="ACGE70_002441"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFF8694046.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1256..2197
                     /gene="ribF"
                     /locus_tag="ACGE70_002442"
     CDS             1256..2197
                     /gene="ribF"
                     /locus_tag="ACGE70_002442"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFF8694047.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2592..3377
                     /locus_tag="ACGE70_002443"
     CDS             2592..3377
                     /locus_tag="ACGE70_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFF8694048.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3390..4241
                     /locus_tag="ACGE70_002444"
     CDS             3390..4241
                     /locus_tag="ACGE70_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFF8694049.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4365..4817)
                     /locus_tag="ACGE70_002445"
     CDS             complement(4365..4817)
                     /locus_tag="ACGE70_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8694050.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
     gene            complement(5282..5650)
                     /locus_tag="ACGE70_002446"
     CDS             complement(5282..5650)
                     /locus_tag="ACGE70_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFF8694051.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
BASE COUNT         1918 a          925 c         1158 g         1704 t
ORIGIN      
        1 attaagaaac tgaaaaaata aataaaaaaa aacggaaaaa agactggaag aatggacagt
       61 cttttttttc gtttttggga agtgaaaacc aaactattta cctgtaagcc acccataaat
      121 ccaatcaaat ggtgcaaaga aaaaatcttc cattctattc cctcctttaa aaaaaacata
      181 acatgatttc tatttacgtg agcatgaatt ttgttacgtt tttattgccg aaaaataaaa
      241 accgttcatt atgatgtcaa attcgtcagt aattgatgca atgtgttata ataattcagt
      301 taaatcaaaa atgaaatgga gaaaagacat ggacgggtta ttaactttat ggaaagaacg
      361 cggaatgaca agtcatgatt gcgtattcaa attaagaaaa atcttacata cgaaaaaaat
      421 cggacatggg ggtacgcttg acccagacgt agacggtgtg ttgcccattt gtattggaaa
      481 agccacaaaa gttatcgaat atttgacaga ttctgggaaa atctatgtag gcgagataac
      541 tctaggtttc agtacaacga cggaagatcg atccggagaa cgggtagaaa caaaaccagt
      601 agatatgtct ttatctgaag aaaaaataga tgaagtgatg gcttcattta ttggtgagat
      661 cacccagata ccaccaatgt actcagcggt aaaagtcaac ggtaagcggt tatatgaata
      721 tgcccgcaat aatgaaacag tggagcgtcc tgttagaaag gctcagattt accgttttat
      781 ccggacaagt gaattgacaa gagacgaagt gacaggaacc atttcttggc gcttcgaagt
      841 agaatgcggg aaaggaacct atgtccgcac attagctgta gatacaggca agaaacttgg
      901 ttaccctgcg cacatgtctg atctgacccg aacagcaagt gccggtatgt cccagaaaga
      961 tgctctgaca ttagaagaag tagctcaagc tgtgaaaaat gggacaatcg atgattgttt
     1021 gctgccaata gaaaaaggag tgaaacgatt caaacgagta gatattactg atgaagtatg
     1081 gcagaaagta aaaaacggta tgagacttaa ttatactgta tttgcgctcc cagaaatgcc
     1141 gtcagaagaa attgctttgt tttataaagg caaagttgtc agtatctatc agccaaaccc
     1201 gaaagaaaaa aacaaattaa aacctagtaa agtattgaga aatgaggaat aacgaatgaa
     1261 tatcatcaag atcagacatc cttaccaatc agaacaaatt ccagaagacg atgtcgtttt
     1321 agtactagga ttttttgacg gcgtgcatat tggacaccag aaagtcataa agacaggtat
     1381 ggaaattgca caaaaagaag gattgaaatt agcattgatg actttcaatc agcatccatc
     1441 gattgtcttt aaaaagatcg atcctagcag cgtgaaatat ttgactagcc ttaagcaaaa
     1501 agaagaaaaa atggccgctt tgggtataga ttatttatat gaagttgatt ttacttcttc
     1561 ttttgctcat ttagcaccgc aggaatttgt tgatcaatac atcgtagggt tacatgcaaa
     1621 atatgctgtt tccggttttg attatacgta tggaccaaaa gacattgcag atgtcgctca
     1681 ttttcctgaa tatgccaaag gaagattcaa aatcgtcaca gtttctaaag aagaagaaca
     1741 aggtgaaaaa atcagctcta cccgtattcg aggattacta gacagcggac aagtaaaaga
     1801 agcagcaaaa ctattaggtt gcacatatga ggtagaaggg gtagttgtcc atggcgatgc
     1861 acgaggcaga ctactaggat tccctacagc aaatgtcaaa gtaaagagca cagttcattt
     1921 gcctaaagaa ggtgtttatg tatcggaaat aaaaattggg gaaatttggt atccagccat
     1981 gggttcaatt ggacataatg atacattcgg tgaaggacgg gagctgactg tagaattgta
     2041 tattttggac tttaatcaag atatctatgg tgaacatgtc gcagtacgct ggaatcaatt
     2101 tttaagagat caagtgaaat tttctagtgt agaggaattg attgaacaac tcaaagagga
     2161 tgaacaagca acagctgaat atttttctca gcaataaaac attatgcata aaatctcttg
     2221 tttatgtata actagcaatt tcaataaaat gggtgataaa taagcccagg aaaggggctg
     2281 tgacaaaagt cttgtcacag cctttttttc caaataaatg gtttttcaga agtagcttct
     2341 tcggaaataa gccgaaattt cacaaaaatt tgagaagtaa tttttgtaaa ttacttctta
     2401 tttcttgaag ctggccactt ctgtcacaac ctctttcttt atagaaataa aaggacacta
     2461 gcaattcatg aaaacgattg ctaaagcgta tcatttgctt tagactatat atcttggtgt
     2521 acactcactt taagacggaa ataaaagtta tccgtaataa caggtttagg tatgaaaaag
     2581 gagtgggtac tatgtataag gaattagaaa acaaagtcgc agtagtaact ggtggctcaa
     2641 aggggattgg aacagcgatt gctcaacgat tcgggaaaga aaaaatgaat gtagttgtca
     2701 actataactc ggataaagaa ggagcagaaa aagcagtcaa agcaatcgaa gaggcaggcg
     2761 gaaaagcagt ctctgtacaa gcagatgtca gttctgaaga aggaatacag cgtttacttg
     2821 atacggcttt agaaaatttt gggacactgg acgtttggat taataatgct gggatggaaa
     2881 atcagcatcc aacccatgaa cttagccttg aagattggga aagagtatta aaagtcaatt
     2941 tgactggcgt ttttatcgga acgaaagcgg ctttaaatta ctttacagag aatgataaaa
     3001 aaggaaatat catcaatatg tcttctgttc atgaacaaat cccatggcca acgtttgctc
     3061 attacgctgc ttcaaaaggc ggagtaaaac tattcaccga aacggtagca atggagtacg
     3121 caccaaaggg aatcagaatc aatagtatag gacctggtgc tatccgtaca cctattaatg
     3181 cggcaaaatt tgatgatcct gaacaaaaag aactcttgga aagtatgatc ccactaggac
     3241 gcgtaggtga accaaaagaa atagcggcga cagctgcatg gttggcttca gacgaatcca
     3301 gttatgttac aggtattaca ctatttgcag atggtgggat gactttatat ccttctttcc
     3361 aaggtggaaa aggataaggg tgaataaaaa tgggtttatt gattgcattg atcccggcag
     3421 ttgggtgggg gatccagcca ctgattttga aaaagattgg tggcagacca acaaatgaga
     3481 tattaggaac tggtattgga gccgtgctcg ttggcttact tgttcaatta ttcgcttctc
     3541 cagctggtat ttcagtgact acatttttga ttagtttact ttctggtgca ttctgggtca
     3601 tcggacaaac aggacagtat acatctttca acttgattgg tgtttctaaa acgatgccaa
     3661 tttcgactgc attacagtta gtcggaacct cattgattgg tgtcttcgct tttggagaat
     3721 ggtctggaag tatgggaaaa atcattggtg cagcagcaat cattcttctt gtatttggtt
     3781 ctgctttaac ggcaatcagt gatgatcctg gaaaaaaagg cggtatcaca aaaggaatca
     3841 gtctattagc atcaacaagt attggttatt gggtttacag tgcattgccc aaactagttg
     3901 atgcaagcgg actttccatt ttctttccgc aaatgatcgg tgtcttttta ggggcagtga
     3961 tttatgtgat tttccgaaaa ccaaccgcat tttctgaaga caagagttgg aaagcaacaa
     4021 tagttggagc catcttcagt gtgtcagcat tggcttatat tttttcagca aatgaaaatg
     4081 gggttgctac tgcatatatc attacacagc taaacgtagt tatctctaca ttaggcggta
     4141 tgtacatcct gcatgaaaat aagacaccaa aagaaaaaaa attaacgatt atcggattat
     4201 tgctgatcgt agtaggaagc atggtcacgg tctttatttg aggataaaga tggtaaaatc
     4261 tcatttatta aaaaaatatt tgtttgaacc aaacagtaag tacttataaa aaaatagcgc
     4321 tagaatttcc aacatctctg gaatttctag cgctttttaa ttgtttaaga tacaagtgaa
     4381 atatctgtct gcatcaataa tttttcattg atccattgac taaaatttac gacatgattg
     4441 aagttaccac cgattagtat ctccgcatga ttgtctttat aaagatcgta ttgtaaaaga
     4501 cggtcttttt tatttgcgta aacatcgtta tggtactgga ctaattgttc tcggatttga
     4561 ttgattgctg taaaatcacg atactgaagg ttgaaacgca tcgtgattgg tgcatttagc
     4621 atgaatcgct gcatttcttt tgctgcttcc tctgcttgat ctccgtatag gaactcaaaa
     4681 agattacgtt cagaaaattt ccattgcccg ccaacctttg ttccttttag ttttccttca
     4741 tgaagataat ttctgataga acgtgtagta acacctaatt gttcggctaa ttcatttact
     4801 gagtaaaaat ctttcaatgt tcaccctcct atgtgaatgg ttctactgac ttagttgata
     4861 tttgcttaat cacgaaatgt agaaagaacg attttaatta aggtcattgt aaaaatcaag
     4921 tcagaaaatt ctaacttttc tttctctaaa gaaattcgtt ctctcctaaa gtgatattat
     4981 atttaaatat acctgaaaat aatgggcagt attatgaaag atcactgata ttttctcgta
     5041 ttttttcctt attcaaaagt tttgcaggaa aattgctgaa aaaataattt tactaagtgg
     5101 aaaattcaaa gccattcaat cagtgatttt tttcagcaca agaaagactc cttataaaaa
     5161 tagcagatac tttgtcaatt aagtgtttaa ccagggatga taaaaaggat aggattgaac
     5221 aaaacctgta taagctgatg aatagtttcc tcgccagtaa aaggtttatt cgtataaagc
     5281 tttatcgcca aaatttatca tgaccttttt tatcttcaat attaaatgaa atcaattgtt
     5341 caatcaattc ccagttgatt acacttttcc aaggaacttt gatgacgtta tccgtatata
     5401 cgtagcctgc ttcttggata tttttttcaa gggcacgaat cgctgctttt tccggtgcga
     5461 tagaaaaatg atttttagca aaactaaatg caataataaa ggtaccatgg tcagtaaaca
     5521 taggttggtt ccattttaca gttgtgtcta gctggggata cgtttctgtt atcttagcaa
     5581 aaaggtcttt gatcttttgt tgatgttctt tttctttgat ctgattgatg atcggctgaa
     5641 atttttccat tctaaaacgc ctccattaag tggtttgatt tattttacca gaaaaacaat
     5701 agtta
//
LOCUS       DAXWOO010000163         5689 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.166, whole genome shotgun sequence.
ACCESSION   DAXWOO010000163 DAXWOO010000000
VERSION     DAXWOO010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.166"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(488..1297)
                     /locus_tag="ACGE70_002447"
     CDS             complement(488..1297)
                     /locus_tag="ACGE70_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFF8694052.1"
                     /translation="MVKESVIGKKELRSIFWRSFFMQSSWSFDKMMAYGQMNGIERSL
                     RKIYPNDDDYYTALQRHTETFNITPHIAPFVMSLSVTMEEENAKNPEFDTDAINKIKT
                     GLMGPLSGIGDSFFWGTFRVIAAGIGISFAQQGNILGMILYFTLYTAIHFIFKWLATK
                     YGYVLGTKFIDDTEDNHMIDKLSHGASILGLTVIGAMIGTMVTLNTSLKFTVGGAETS
                     LQSIFDQIFPGLLPLGITFLCVWLYSKGHKTITIILGIFVLCFLGTLGGIF"
     gene            complement(1297..2043)
                     /locus_tag="ACGE70_002448"
     CDS             complement(1297..2043)
                     /locus_tag="ACGE70_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFF8694053.1"
                     /translation="MIQAILIALVAFLASIDEYTFGASMMGRPLFTAPFVGLILGDLE
                     SGIIIGATLESMFMGSIMVGSATPPEVYASSVLGTAIAIQSGQGVGTAVALTLPLSIF
                     LQMWRNFAYAMPITYFNNKMDEALVNHDFKKAQKYHYVAQPLIVGVPSAILVFVATYF
                     GVSTINAMIKLIPQVVLDSFDVAAGVLSCVGLALLIKMMANKAMMPYLFLGFVGVMYL
                     KMDVVGVAVVGLCLALLAVQKLTFEEEEDL"
     gene            complement(2061..2528)
                     /locus_tag="ACGE70_002449"
     CDS             complement(2061..2528)
                     /locus_tag="ACGE70_002449"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFF8694054.1"
                     /translation="MIKLLRVDERFVHGQVAFAWTNNLGANCIFVASNEIATNNLRKK
                     TLKMAVPTGTKFVVKDMTEAISVLNGEKIKKYQVFLIVGTVEDAYEITQHVPEIKEVN
                     LGNLKKNENRKMITSSVYLNEQELDLVKKMTENGITVQCQAVPTDKKINPLHD"
     gene            complement(2528..2938)
                     /locus_tag="ACGE70_002450"
     CDS             complement(2528..2938)
                     /locus_tag="ACGE70_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFF8694055.1"
                     /translation="MRKIVIATHSMMAKGLKDTLEFIIGPQKSLLAITAYIDSDYSIN
                     QEIEQLFSDLAPDDELIVTVDLLGGSVSNAFSEQIGKDNFYLISGVNLPLLLELCLSG
                     EQDTKKLIQKAMNSGNEGIVLVNELLEKTEEDDF"
     gene            complement(2949..3962)
                     /locus_tag="ACGE70_002451"
     CDS             complement(2949..3962)
                     /locus_tag="ACGE70_002451"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFF8694056.1"
                     /translation="MLGFSEERFLSVLNGAVKNIETINQVADAISEKGFKNLYLVGSG
                     GTYATISPLAYLLSTHSELDYYHEIAAEVVCSQPKKINKDTVLITSSLTGTTQETVDV
                     VKYANSQGATTIAFVGNAATPLGEIVDYAIENDADNDTLVEELQLQFFALGARLMKNN
                     GEFPEYDEFIQTLQKMPEVLLSVRQQNDQKALEFAEKHKNTEFHMFVGAGNTYGAAYE
                     YAMCVLEEMQWIATKSIHAAEFFHGTIEMTDKSMSFVLLKGEDQTRPLVDRVERFVNK
                     YSDVVTVFDTADFELAGVPSAMRQYVAPMVMATALERVSAHFEKVRDHSLDIRRYYRT
                     VEY"
     gene            4179..4913
                     /locus_tag="ACGE70_002452"
     CDS             4179..4913
                     /locus_tag="ACGE70_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFF8694057.1"
                     /translation="MNTEELTNKLIQDIEQEKYKYAQAIPSERDFVQIYEMNRSAVKK
                     AIQQLVNKEYLLKIHGKGTFVKKNIHEHLQIKFHGLSELLEREGIQPSSLILASEIKP
                     AGHIVGKALQLEPTEEVFHLVRLRSGDGEPISIEDTYIPLNLIPDLQAFDFQIFSFYS
                     TLAAYHYQIENIYQTISSSKVSNKEARLLKTSLNRPVISLAITAVTTNEQAVEYTEVV
                     VLPEFAAIYTKNVHQGKEMKIYAQID"
     gene            4897..5607
                     /locus_tag="ACGE70_002453"
     CDS             4897..5607
                     /locus_tag="ACGE70_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFF8694058.1"
                     /translation="MPKLINLLAYDLIDKIIAYIHEHHLTPGTKLPSERDLSEMFNVN
                     RTTLRGALQKLVDNGTLRAQTNSGYYLTAPKFEKNAHHFFTPIEDSLLSKQRLQLKAT
                     ALSPKKAAALCPELNDFKQQQPQAYLEYIDAEAISLMLLIPMETLKKSNFSDNPFQDF
                     KSLAYTRSQEISLITDNDVAADLLGVDKEEVLLFVREEIRAGKELAAIALGYTISRRC
                     NIQVNVATTLQSGSVAKF"
BASE COUNT         1832 a         1195 c          943 g         1719 t
ORIGIN      
        1 aagcgacaaa aaccacgagc aggtccattc atcctcaaac ctcggtgatc ttcgcggttt
       61 aacaccatca gaagaaatgc acaaaaataa taaggagagt ctgagacata aataaattcc
      121 cagactttta gtgagtctaa atccgaataa acggtgggtc gaaagcaact ctagctgcgc
      181 gtcgatcgca gcaatctcta ggtgctatgt tcggataaat ggtggtcaaa agtcagattt
      241 tcggccattt cgtaacagcc aaccagacaa aaagcgtctg ttttgtcttc tactccaatt
      301 gctcaaatct tagcgacttt tggcttggtc ttactctaaa cagtttgtaa gcctaaactt
      361 tatttgctgt ttgtttctaa gatcaaagca aaactacttt ttagttggtt acttttacaa
      421 gttcttttta ctgttgaata aatgtaaatc ttaagaacaa atatttactt ctataattcc
      481 aagtttctta aaaaattccg ccaagtgtac ctaaaaagca taaaacaaaa attcctagaa
      541 taattgtgat cgttttatgc cctttggagt aaagccagac gcataaaaag gtaattccta
      601 atggcagcaa gcccggaaaa atttgatcaa aaatactttg taatgaagtt tcagctccac
      661 caaccgtgaa ttttagtgac gtatttaaag taaccatcgt gcctatcatc gcgccaatta
      721 ccgttaaacc taaaatcgaa gcaccatgag ataatttgtc aatcatatgg ttgtcttctg
      781 tatcatcaat aaatttagtt cctaaaacat agccatattt tgtcgccaac catttaaaaa
      841 taaaatgaat ggcagtatac aaagtaaaat aaaggatcat gcctaaaata ttcccttgtt
      901 gagcaaaaga aatacctatg ccagccgcaa taacccgaaa agttccccag aaaaatgaat
      961 cgccaatacc tgataaaggt cccattaatc ctgttttgat tttattaatt gcgtctgtat
     1021 caaattcagg atttttagca ttttcttctt ccatggtgac ggacaagctc ataacaaaag
     1081 gcgcaatatg cggtgtaatg ttaaatgttt ccgtatggcg ctgcaacgct gtataataat
     1141 catcatcgtt cggatatatt ttacgtaagg aacgttcaat accattcatt tgtccatatg
     1201 ccatcatttt atcgaaggac caagaggatt gcataaaaaa ggagcgccaa aatatacttc
     1261 ttaattcttt tttcccaata acactttctt ttaccattat aaatcttcct cctcttcaaa
     1321 agttagcttt tgaacagcca gtaacgctaa gcaaagtccg acgacagcca caccgacaac
     1381 atccattttc agatacatta cgccgacaaa tcctaagaaa agataaggca tcatcgcttt
     1441 attcgccatc attttaatta ataacgctag cccaacacaa gaaagcacac cggccgcaac
     1501 atcaaagctg tccaatacaa cctgaggaat caacttgatc atcgcattga ttgtagacac
     1561 accaaaataa gtagctacaa aaactaaaat ggccgatgga accccaacga ttaaaggttg
     1621 agccacataa tgatattttt gtgctttttt gaaatcatga ttgactaatg cctcatccat
     1681 tttgttgtta aaatacgtaa ttggcattgc ataggcgaag ttacgccaca tctgaagaaa
     1741 gatggacaaa ggtaaagtca acgcaacggc agttcctact ccttgacccg attgaatcgc
     1801 aattgctgta cctaaaacac tggaagcata aacttcaggg ggcgtcgcac tccctaccat
     1861 aattgagccc atgaacatgg attccaaggt ggcaccaata ataattcctg actctaaatc
     1921 tcccaaaatc aaaccgacaa aaggcgctgt aaatagcggt ctacccatca tcgatgcacc
     1981 aaatgtgtat tcatcaatag acgccaaaaa agctaccagt gcgattaata ttgcttgtat
     2041 catattcttc ccctcctctt ttagtcatgc aaaggattga tttttttgtc cgttggtaca
     2101 gcctgacatt gaactgttat cccgttttca gtcatttttt tcaccaagtc cagttcttgt
     2161 tcattcaagt aaacggaact agtaatcatt ttccgattct cgtttttctt taaatttcct
     2221 aaattcactt ctttaatctc aggtacgtgc tgagtaattt cgtaggcatc ctccaccgtc
     2281 ccgacaatta aaaatacttg gtatttcttg attttttctc catttaaaac agagatggcc
     2341 tctgtcatat ccttcaccac aaattttgtc cctgtaggaa cagccatttt tagtgttttt
     2401 ttgcgcagat tattggtagc gatttcatta cttgcaacga aaatacaatt tgcacctaag
     2461 ttatttgtcc aggcaaaagc aacttgccca tgaacaaaac gttcatctac tcgtaataat
     2521 ttaatcatta aaaatcatcc tcttctgttt tctctagcaa ctcgttcacc aaaactatcc
     2581 cttcattccc gctattcata gctttttgga ttagtttttt cgtgtcctgt tcacctgata
     2641 agcaaagctc taaaagcagc ggcagattta caccgctgat taaataaaaa ttgtctttac
     2701 cgatttgttc ggagaaagcg ttagacacgc ttcctcccag taaatctacc gtcacaatta
     2761 attcgtcatc aggcgctaag tcggaaaata gctgctctat ttcctggtta atggagtagt
     2821 cactatctat atacgcagtt attgctaaaa gtgatttttg aggaccaata ataaattcta
     2881 aagtatcctt taagccttta gccatcatgg aatgcgtcgc aattacaatt ttcctcatag
     2941 ctcttacctt aatattccac agtccgataa taacgacgaa tatctagtga atgatcccgc
     3001 accttttcaa aatgagcact cactcgttct aaggccgttg ccataaccat tggcgctaca
     3061 tactgacgca tagcgcttgg tacacctgct aattcgaaat ccgcagtgtc aaaaaccgtc
     3121 acaacatcgc tatatttatt tacaaaacgc tctacgcgat cgactagcgg acgcgtttga
     3181 tcttcacctt ttaataacac aaaactcata gatttatcag tcatttcaat agtcccatgg
     3241 aaaaattcgg ctgcatggat cgacttggtg gcaatccatt gcatttcttc cagtacgcac
     3301 atagcatatt cataggcagc cccataagta ttcccggctc caacaaacat atgaaattcc
     3361 gtatttttat gtttttctgc aaattcaagt gctttctgat cattttgttg ccgaacactt
     3421 agtaatactt ccggcatttt ttgtaatgtt tgaataaatt catcatattc aggaaactca
     3481 ccgttatttt tcatcaggcg tgcacccaaa gcaaaaaact gtaactgaag ttcttctact
     3541 aatgtatcgt tgtcagcatc attttcaatc gcataatcca cgatttcccc gagtggtgta
     3601 gcagcatttc ctacaaacgc aatcgtcgtt gcaccttgac tattggcata cttaacaaca
     3661 tcaacggttt cttgagtcgt accggttaat gaagatgtga ttaacactgt atctttatta
     3721 atctttttcg gctgtgaaca aaccacctct gcggctattt catgataata atcaagctct
     3781 gaatgagtgc ttaacagata agctagcggg ctgattgtgg catatgttcc accagagcct
     3841 accaaataaa gatttttaaa tcccttctca ctgattgcat ctgccacctg attgattgtc
     3901 tcgatatttt ttactgcacc atttaatact gatagaaaac gttcttcgct aaaacctaac
     3961 atagttaaaa acctccaagt caataatttg aaaaatatat ttgcaaatgc cggcacatta
     4021 acaaaccact taaccttttt tatataccac ctgttaacgt ttttaatgta ccacttatag
     4081 aaaacgctgt caatagtttt taaccacttt tatttttatt tgtaccaaaa tccttttttc
     4141 atgtataata aacaaaaata gactctggag tgtaacgtat gaatactgaa gaactaacga
     4201 acaaattaat acaagacatt gaacaagaaa aatataaata cgcgcaagct attccttcag
     4261 aacgtgattt tgttcaaatt tatgaaatga atcgttcagc cgtaaaaaaa gccattcaac
     4321 aattagtaaa taaagagtac ctactaaaaa tacatggcaa aggaactttt gttaaaaaaa
     4381 atatccatga acatttgcaa atcaaatttc atggtttgtc tgaattgttg gaacgggaag
     4441 ggattcagcc tagcagctta attttagctt cagaaataaa accggctgga cacattgtcg
     4501 ggaaagcttt acagctggaa ccaactgaag aagtatttca tttggttcgt ttaagaagcg
     4561 gcgacggtga gccaatttct attgaagaca cttatattcc attaaatctc atacctgatc
     4621 ttcaagcttt tgatttccaa attttttcat tttattccac attggctgct tatcattatc
     4681 aaattgaaaa tatctatcag acaatttcca gctcaaaagt gagcaataaa gaagcccgtt
     4741 tgttaaaaac cagcttaaat cgtccggtaa tcagccttgc tatcactgcg gttacgacaa
     4801 atgaacaagc ggttgaatac actgaagtag tggttttgcc tgaattcgcc gccatctata
     4861 ctaaaaatgt tcatcaagga aaggagatga aaatctatgc ccaaattgat taatctgttg
     4921 gcttatgacc tgattgacaa aattattgct tatattcatg aacatcattt aacccccgga
     4981 acaaaactgc cttcggaacg ggacctatca gaaatgttta atgttaatcg aactacctta
     5041 agaggcgcgt tacaaaaact ggtggacaac ggcaccttac gggcgcaaac caatagcggc
     5101 tattatttaa ctgcgccaaa attcgaaaaa aatgcccatc atttctttac accgatagaa
     5161 gattctctgc ttagtaagca gcggctacag ctaaaggcaa cggctctttc accgaaaaaa
     5221 gcagcagctc tttgtccgga actgaatgat ttcaaacagc agcaaccaca agcttattta
     5281 gaatatattg atgcagaagc aattagcttg atgttattaa tccctatgga aacattaaag
     5341 aaaagtaatt tctcggacaa tcctttccaa gattttaaaa gtttggcata tacgcgttct
     5401 caagaaattt ctttaataac tgacaatgac gttgcggcgg acttgctggg agttgacaaa
     5461 gaagaggttc tgttatttgt acgagaggaa ataagagccg gtaaggagtt ggcggcgatt
     5521 gctttaggtt atactattag tcgccgctgc aacatacagg ttaatgttgc tacgaccttg
     5581 caatccggtt ctgttgcaaa gttttaaatc tactatcaga taaggtagaa taatagaaaa
     5641 agatagcagg aggaatgacg atgagtcatt ttaaaggaaa gcaatttca
//
LOCUS       DAXWOO010000164         5589 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.167, whole genome shotgun sequence.
ACCESSION   DAXWOO010000164 DAXWOO010000000
VERSION     DAXWOO010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.167"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(197..700)
                     /locus_tag="ACGE70_002454"
     CDS             complement(197..700)
                     /locus_tag="ACGE70_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFF8694059.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1059..2084
                     /locus_tag="ACGE70_002455"
     CDS             1059..2084
                     /locus_tag="ACGE70_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFF8694060.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2407..3286
                     /locus_tag="ACGE70_002456"
                     /pseudo
     CDS             2407..3286
                     /locus_tag="ACGE70_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
     gene            3762..3878
                     /locus_tag="ACGE70_002457"
     CDS             3762..3878
                     /locus_tag="ACGE70_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFF8694061.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            4806..5144
                     /locus_tag="ACGE70_002458"
     CDS             4806..5144
                     /locus_tag="ACGE70_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694062.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5135..5293
                     /locus_tag="ACGE70_002459"
     CDS             5135..5293
                     /locus_tag="ACGE70_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694063.1"
                     /translation="MDVDTSEILYDIEIDENLLDVAQEIDHQKQEAIRYAGRKGKRKY
                     LLRECSKS"
     gene            5274..5468
                     /locus_tag="ACGE70_002460"
     CDS             5274..5468
                     /locus_tag="ACGE70_002460"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694064.1"
                     /translation="MNVAKVREDENEWKEFKSRYSINSTPTFTVYREGSIEKTVFWTK
                     ESGMSLAEVEEFLDYVSMQQ"
BASE COUNT         1879 a          845 c         1031 g         1834 t
ORIGIN      
        1 ctttaagcta aaaaaaaacc ctttttgtta aataagggga atatagcctg tcagtttcaa
       61 actgcaagtc gtcctgctgc aataatatag tagattagag atgaaaatag atttttttaa
      121 accaatgact gaataaaaag accgtcatag atacacattg gaatgtgtct attacggtct
      181 ttttttacga acgattttat tgaagacgga cagttcgggt caaaaataaa aaagctacga
      241 tcagaaataa gaataaaatg tatcccaaaa taatggctag acggtctaac ttatttgtcg
      301 atagatgttt gtttagaagt tgataggccc ataaacctat cagtcctcct gttgtattag
      361 ttatcaaatc agtgatgtcc gctacgccaa taccgaatag gaattgaatc aattctgcca
      421 agaagcttac aaggaaaata atccctaatt ttttagaaaa agaaagtctc ttgaaattga
      481 cattcaaaag caccccaaaa ggaaaaaaga caatcaagtt ataagtggtc tcacgtaata
      541 ccgtcgtatt ttcaaaagaa aaagtagaaa aagggacgag attgacattg ctgtaagaat
      601 aagtgagaaa ttgtggaaga ttaaatgata gcttgaataa aatcaaccat aatagaaaga
      661 ataaataaat ccctaataaa aattttgata cataccgcat agacatctcc tccatttaaa
      721 aagtatacca tcgaaaaccg ctgaaaaatc ataaaaaagt atgaagttta aggaccaacg
      781 tagttatcgt acgaaaatta gaagcttttt tttgttggga atccggtttc tttaacaaag
      841 ctaagatagc gagttgcttg gaaaagttgc tatctttttt ctgtcaaaac gactcggaat
      901 gttcgttttt tgttctaatt tctccaagtt gtttactttt tgatactaag tttatgaaaa
      961 atcaagaaat agttgttata ctaggtaatg ctgacaaacg ggattggcat ttcatcttgg
     1021 ttcagctttt tttccaatta ctaggaagga gggatagatt gttcaagact tattttcgtt
     1081 tcaataaaat acaagataat ccttttcgta tcatgaatct gatgctactt atctttattg
     1141 tattatggtt aggttacata aatcatgatt ttttgatttc aagtttttca tcattaggga
     1201 tctttacttt tttctattat caaaacatac cgatgaaata tttgctgaaa cgtatgtttc
     1261 tgatcggttg cggactgttg gttgctttcg ttttggggat cttatcgact tatgttttat
     1321 ggcttgaacc atttgccgta gctttggttg ctttttccag cagattcgtg ttgcgattat
     1381 tccatatttc taaaccaggt ggcttgtttt ttgcgatgct ttctgccatg ggaacgagca
     1441 tgcagctgcc aatcgctcaa ttaccgatcg tctcacttta tttttttatg ggtgtcgttt
     1501 ttgccctgat tgcggcagtg attacaaaat tactggattc acggcctgag cagacaattg
     1561 aaaaagcaac tttaaaagaa agatttcacg aagagccttt agtgatcatt gattctgttt
     1621 tctacagcgc tgccttattt ctttccgttt atgtcagtca cggcttgaat ctgcataatc
     1681 cttattggtt gaccttgtca tgtgcatcga ttttgctagc agaaaacttg gatgcgatga
     1741 agcatcgaca agtccaatat ctgattggat cgatgggtgg gttatgtgtg tcggcattct
     1801 tgtcatttgt gccgtttacg cagttgcaga ccatttttct gatcactttt ctatatggaa
     1861 tcgcacaatt tctagttgct agaaattatg cagttgctaa tatttttctt aatccgatgg
     1921 cgttgatgct atctacactt attcgtggag catacttgat ttctttgatc gaatatcgtt
     1981 ttttagggat cgtcatagga agtttcatcg gtttaggtgt tgcgtgggta atgacggttg
     2041 gtttgcaaca ttatttaacg gttgttcgtc aaaaattaga atagaaggac aaatatatgt
     2101 gccaaaagag gttatgacaa gatttttgtc acaacctcta attcaaggtg ttcaaaagcc
     2161 agattctcag cattaagtca aattcacaca aaaatgtgga aaggaacttt caaaaaaatt
     2221 ttatatttat tctatcaaaa aattttgttt atgatggtaa ctgaataaac tttcctgaga
     2281 tggtcaatgc tttccttgaa agtcttacag atattctttc ttcatagaaa gtttaagttt
     2341 tatagttagt gtacaataaa agtatgttta taccatatgg tgtccggata atcagtcatt
     2401 taaacaatga ggttcaaaga tatgaaaagg ggaaatttga tgaagaaaat aagcaatgaa
     2461 atccaaattc tggcagaaac tttaactctt cccaaagtcg ttatagagca aatgattgta
     2521 caagcaaaaa acatccttga tgaggtgttt gagcaatata gtcatgaact aatggatcct
     2581 aataaagcca aaggcgactg caaagaaaat agacaaagtc tatgaagagg aaccttttgc
     2641 agcattagct atttatctat atgcagcttc tcaaagttgg aaaaaaattt atcaaccatt
     2701 gaagattcca agaactatct atttggcaac aatgaatgct tttactagat ttattcatga
     2761 acatttccaa gtaacaggaa gctatcgctt tgaccgtggt ttttggattt ggcattacac
     2821 tagtggcttg atctttcgga ttggtgaatt ggagtacgaa atgacatatt ttccgaaagg
     2881 gtacacggat ctaagattag aaggaaagca gtgcctttct cttcatattc cttccgatgc
     2941 agatttatct cagaataaga taagagatag ttacgaaagt gccattcgtt tttttgaact
     3001 ttattttcct gattaccatt accaagtgat gtacacagat acttggttac tatcacccaa
     3061 tcttactaaa tggttgaaaa aagagtcaaa aatctgtctt tttgcagccg attatcgatt
     3121 attatcagtt gatgaacagg acgatagtgg agtcccttgg atctttggac gagtagatgc
     3181 ccaaatacat gattatccag aaagtacgag tttacagcga caagcaaaag agcaattgct
     3241 tgcaggcgag cacattggct ctggcttagg tattcttact tcataagttt agtacttcga
     3301 aatagtttaa aagagcgtga gacaaaagta aagaacactt ttgtctcacg ctctaaacac
     3361 gtataaacgg cggaagcaga agcaactcct tcggaaataa gctgaaattc acaaaaattt
     3421 gaaaagcaat tttcgtgaat ttcttcttat ttcttggagt taaacgcttc tgtcccagcc
     3481 tcttttttag tgttctatcg aacacacagt acgttcaata ggtaaactag ctataacaat
     3541 agatattaaa gttaacggac aagagggcct tttttaggta gctaaaacgt aataatgaaa
     3601 atttatttcc agagatagga gtcaatcagc tagtctgatc gactgtttta acaagttgac
     3661 tttcatttta acttttgata ccatttaaaa cacaaatcgt aattgtttcg atttgtgttt
     3721 tttagcatga ctacaatcaa agaagaaagt gggtcgtcca tatggaaaaa acagatatct
     3781 caagtgcata ccgccgcttg aaaagcccaa atatcaaaac acgaaaacgt gcgctgaaga
     3841 tcatcaaaga tgtgaaacgg aatagtggga aaaggtaaga agaaggaaac ttaattaaaa
     3901 aaatagccga aaaacgtttt tcggctattt tcaataatcg gtttttgtgt aaggtttccg
     3961 aaagaactgc tgttgatttt ccatttttaa catatgataa attggtagaa tctttctttt
     4021 caaattgata ccattcccta attccagtta tatagttaac tacagaacca caaagcacgt
     4081 acgtttataa aagtattatc tgtagttatt tttcaattcc ctttcaacct cttattttga
     4141 ccagtagtat tgatcacttt accttttaac acctttgttc gtttttgaaa catttttagt
     4201 caagaatgga aacgtaacta cataatcaaa ggttttgcga aatatttcat gaaatatttc
     4261 atgtagaagc gagcaatacg gttgcaaata accattatcg tttaataagc agttacgaaa
     4321 gctcccaata tctctaacgt tcagctcact ccatttcttt tcttcaaaaa aatcagtgat
     4381 tcgtatctct ttctatttgt cgaaaaagtt aactttaaaa agtaacgccg aaaaaatatg
     4441 caaaaaaata aaaaagcagt tactattgct agaataacat caaggaataa taagttttgg
     4501 ttttcagata atagtcgata agttgtgtat tggaatttta gatacagtaa taaagtattt
     4561 ttcatgaaat tttgtgagaa tcactattac aagtatcttt aataaaatta atggttaagg
     4621 aggaacttaa agtagaatgt aaatgaatag ttgtagtcag tcgattgtga tactagactc
     4681 aaagttgcag gaataagttt ttcaatttgt gattggaaga taaaaaatga aaaagaaggg
     4741 ctttttttta tactttgttg attgtagtgt ttattttccc tctgtttagc caagtaacat
     4801 tttctatgac tcaaccagtt tcaagtgagg aacatgataa cctttttcgt gctacagaag
     4861 agtttaagaa atatcaggaa aatattgttc cggagactcc attagctagt gagccaatta
     4921 ctgatattga tggaaaccaa ataggatggt atatacagta cgaagttatg gtggtaaata
     4981 atgactcaat agtaaaaaat acagtaggct tagagctaac accgattgaa aaggtgagtt
     5041 cattgttaac gtatatttat actgatgaaa gtgacgagac tgagatcatt ttaacggatt
     5101 actcaaaaat tgctactgat aaaaagtata tattgtggat gtagacacta gtgaaatact
     5161 ttatgatata gaaattgatg aaaatttact agatgtagca caagaaatcg atcatcaaaa
     5221 gcaggaagct attcgatatg ctggtagaaa agggaaaaga aaatatttat tacgtgaatg
     5281 tagcaaaagt tagagaagat gaaaatgagt ggaaagaatt caagagtcgc tactcaatta
     5341 actcgactcc gacatttaca gtttacagag aaggatcgat agaaaaaact gttttttgga
     5401 ctaaagaaag tggaatgtct ttagctgaag tagaagagtt tttggattat gttagtatgc
     5461 aacagtaagg gagttcttat tatgaaaaat tttgacacat attatgttat tgttttttcg
     5521 atatctttgc tgttaatatt actaacttta atgcagtata aagagtatcc agagtatttt
     5581 attttaact
//
LOCUS       DAXWOO010000165         5488 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.168, whole genome shotgun sequence.
ACCESSION   DAXWOO010000165 DAXWOO010000000
VERSION     DAXWOO010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.168"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            82..1371
                     /gene="tig"
                     /locus_tag="ACGE70_002461"
     CDS             82..1371
                     /gene="tig"
                     /locus_tag="ACGE70_002461"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFF8694065.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            1618..2868
                     /gene="clpX"
                     /locus_tag="ACGE70_002462"
     CDS             1618..2868
                     /gene="clpX"
                     /locus_tag="ACGE70_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFF8694066.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            2951..3538
                     /gene="yihA"
                     /locus_tag="ACGE70_002463"
     CDS             2951..3538
                     /gene="yihA"
                     /locus_tag="ACGE70_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFF8694067.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(3612..3767)
                     /locus_tag="ACGE70_002464"
     CDS             complement(3612..3767)
                     /locus_tag="ACGE70_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFF8694068.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            3903..5231
                     /locus_tag="ACGE70_002465"
     CDS             3903..5231
                     /locus_tag="ACGE70_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694069.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            5242..5451
                     /locus_tag="ACGE70_002466"
     CDS             5242..5451
                     /locus_tag="ACGE70_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694070.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
BASE COUNT         1924 a          909 c         1121 g         1534 t
ORIGIN      
        1 aaagggttag tgtactattt aaaggtatgc atgaaacgtt tttgatgtat aaaatgaatt
       61 tctaaactcg gagggaatta aatgactgca acatttgaaa aaaaaggcac gaatgatggt
      121 gttttaaaat ttacgattgc gcaagacgaa atccaaaaag gattaacaac tgcgtttaat
      181 aaagtgaaaa aaagcttgaa cgtacctgga ttccgtaaag ggaaagtttc tcgtcaagta
      241 ttcaaccgta tgtacggtga agaagcttta tatgaagatg cattgaatgc tgtattacca
      301 gaagcttatg aagctgctgt caaagaagct ggcatcgatc ctgtttctca accaaaaatc
      361 gatgttgaaa gcatggaaaa aggtcaagac tgggtaatca ctgctgaagt aacagtgaaa
      421 ccagaagtaa aattaggaca atataaagat ctagttgttg aaaaacaaga ccgtgaagta
      481 acagatgctg acgtggatgc acgtatcaaa cgtgaacaag aagcacaagc tgaattagtt
      541 atcaaagaag atgctgcagc tgaaaatggc gatacagtag taatcgactt cgaaggcttt
      601 gtagatggcg aagcgtttga tggcggaaaa ggcgaaaatt actcacttga attaggttca
      661 ggctcattca ttccaggttt tgaagatcaa ttagttggtc ataaatcagg tgaaagtcta
      721 gatgtaaatg taactttccc agaagattat caagcagaag atcttgctgg aaaagaagca
      781 gtcttcaaag tgacaatcca tgaagtaaaa gcaaaagaag tgcctgaatt agatgacgaa
      841 ttcgcaaaag atgttgatga cgaagtatca acattagacg aattgaaaga aaaatatcgc
      901 aaagaattag tagaaacaaa agaaaaagca gcggacgatg caaaagatga agcagcgatc
      961 cgtatggctg ttgaaaacgc agaaatcgtt gaattgccac acgtgatggt tcatgatgaa
     1021 gtacatcgtt caatggacga attcttgaac aacatgcaac gtcaaggtat ctctcctgaa
     1081 atgtactatc aattaactgg ttctacagaa gaagacttgc ataaacaatt cgaaggtgaa
     1141 gcagaaatgc gcacaagaac aaatcttgtg atcgaagcaa ttgcagctgc tgaaaatctt
     1201 caagcaacag aagaagaaat caatgcagaa atccaagaat tatcagaaac atacaacatg
     1261 ccaatcgaac aaatcaaacg cgtgttgaca gaagatatgc tggaacatga tgtgacaatg
     1321 aaaaaagcaa tcgaattgat cacaggtaca gcgacagaag cgaaagaata gaaatcaagt
     1381 aataaatcat ttctgaagaa gctacttcta gaaaattgtt tattcgaaat aagggactgt
     1441 gacacgattt tgtcacagtt ccttattttt tgttaagagt aaaaaatgca aaggtatagg
     1501 tcagttgatc ctttgttaaa cactttttct ttttactaaa ctatgttatg atagtaaaag
     1561 actgtaaagt ctttgatagt ttcttgcata ctttttgcaa gttaaggggt gaagaccatg
     1621 tatgacaatc caagtagcaa tgagaccgta cgctgttctt tttgcgggaa aacccaagaa
     1681 gaagtgaaaa agatcgtagc tggtccaggg gtctacatct gtaatgaatg tatcgaccta
     1741 tgtaaagaaa tcattgatga agaattttat gaagaagctg tacgcgaatt cactgaagtc
     1801 cctaaaccac tagaaattct tgaagtattg aataactacg tgattggaca agatcgcgca
     1861 aaacgctcgt tagctgttgc cgtatacaat cactataaac gtgtcaatca gcaagcacaa
     1921 gaaaacacag atgatgttga actgcaaaaa agcaatatct gcttgatcgg acctacaggg
     1981 tctggtaaaa catttttagc acaaacatta gcaaaaacat tgaatgtacc ttttgcaatc
     2041 gcagatgcga caagtttgac agaagctggt tatgtggggg aagatgtaga aaacattttg
     2101 cttaaattgc ttcaatctgc tgattacaat gtggaacgtg cagaaaaagg gatcatctat
     2161 attgatgaga tcgataaaat tgcgcgtaaa agcgaaaatg tatctattac tcgtgacgtt
     2221 tccggcgaag gtgtccaaca agccttgctg aaaattctag aaggaactgt tgcaagcgtg
     2281 ccacctcagg gaggacgtaa acatccacat caagaattta ttcagattga tacgacgaac
     2341 atattgttca tcgtaggtgg tgcatttgat ggcatcgaaa cgattgtgaa gaatcgtatg
     2401 ggcgaaaaga cgattggatt tggtacacaa aaccaaaaac tttctgaaga cgaaagtgtc
     2461 atgcagcata tcattcctga agatttacta aaattcggat tgatccctga gtttatcgga
     2521 cgattacctg ttatggcagc tttagagaaa ttgacaacag atgatctggt acggatcttg
     2581 actgagccga aaaatgcgct agtcaaacaa tatcaaaaac ttctttctct agataatacc
     2641 gaacttgaat tcgaacctga agcgttgaga gccattgcga aaaaagcaat cgaacgcaat
     2701 accggtgctc gaggacttcg ttccatcatt gaagaaatca tgatggatgt gatgtttgat
     2761 atcccttcaa atgaaaacat cgaaaaagtg attatcacaa aagaagcagc tgaattgtct
     2821 ggtgagccaa cagtaatcta taaagattca gagaaaaaag cgggttgatg aaaagaggct
     2881 gccgtaaaag tcattttggc ttttgcgaca gccgcttgct ttattcatca ccttgttaag
     2941 gagagagacc atgaaagtcc atcaagctga aattatcata agtgcagtgg caccaaaaca
     3001 atatccagat acacaattgc cagaaatagc tctggcagga cgatcaaatg tagggaaatc
     3061 ttcttttatc aacacattga ttgatcgaaa gaatttagcc cgtacttccg gtaagccagg
     3121 aaagacccag acattgaatt tttatttgat cgagaacgca ttacattttg tcgatgttcc
     3181 gggatatggg tatgcaaaag tatccaaaac tgagcgagca aaatggggaa agatgatcga
     3241 gacatattta acgactagag accagctacg tgcggtcgtt tctttagtag atttgcgtca
     3301 taagccttct caagaagata tccagatgta tcaatttttg aagtattatg atatccctgt
     3361 aattgttgtt gccactaagg cagataagat ccctcgtggt aaatggaaca agcatgagag
     3421 cgaaatcaaa aaagcgttgg attttgatcg caatgacacg tttattctct tttcttccgt
     3481 tacaaaacag ggaaaagaag aagcgtgggc agcaatcgaa caagcaattg aatcataaaa
     3541 agaataaaaa agacttacca gtcaaactct ttcataaaag tttggctggt aagtcttttt
     3601 ttgagcttaa cttacttttg tttcttgtct tttttctttt cattttgtgg ctgtggtttt
     3661 agaaatcgat cgatctcttc ttttggtgca actgtttttt ccggatcgac agcaaatgat
     3721 tcgaaaagtt tatctaaatt atctgtacgt tcaaatttca accccattgt aaacacctct
     3781 ttgtttcttt ttacttagtc tatcatgtaa tcgtccaaat tggtttctaa gacataaagt
     3841 tttagttttt ctttatcgtt ggttataatt aaattataaa aagattttaa agaggtgaga
     3901 aaatgattgg cgaagataaa ttattgatga ctatccagct cacagactta aatgaagaag
     3961 aaaaaaaaca ggaaataagc gattgggcta ttttgacacg tctgttgatc cagccaacat
     4021 ttatccgctc tttcacacaa caagcggatc cgtatgatct tcgaaagctt caagaaaaaa
     4081 taatgctagc atcggtaaga gacgaaagtt ggttagttgt aggaaatgac gagaacgaat
     4141 gtagttttcg cttggaagat aatcaattat tgataaaaaa tgtattgtct atctctgttt
     4201 tcaaagaaaa gcaatttttg attcgagact tcattcagaa aaaaatgatt gctcatgggg
     4261 tgttcgccta tatgcgagct tatagcgagt ttatctatca caatacaaaa cagatctctc
     4321 aacggctatt gtttgagaaa aaggatgaaa tcgaacattt acctaaaatg aaacaacaaa
     4381 acggggaagt agtagtcgat tgcaaccaat ttccaggata cgatcttttt taccaaggtt
     4441 tatgtttcac ttcgtgttgg gaaatgtatt atagtcgcta ttatcatcaa atcatcccta
     4501 aaccgatttt tctcgacatc caacaagtag aaaaagtgaa ggaattagaa aacgaagtca
     4561 tatgtatcca gctttatcga gaccccttta actggcaaaa gccaaacaat caaatgtttc
     4621 aatcctattt tagggaccag cttgggtttg atcatcttgc ttgggacaat ggtgtcggtt
     4681 tattgaaacc gccattcgtt gaatacattt acactgacca tacgatccaa tctgtccagt
     4741 atcagaatgc acaaatgcaa cctgttccta aaaagaatgc ttcatttttt gtaacaaaaa
     4801 gttatgatat tcaacaagga gattataaag aaaggcgtgt tcgcggaaca ctaaatgctc
     4861 aggcgtattt tccttgggtt gacgagaatc gctcgcgcat gatgtgctat aaagttatcg
     4921 atccgactgt tgctttggat aatggaatcg aagcttattg ttattatatt agggaatatc
     4981 tagaagtaga ggtaacagat gaaaaatatc aagagtatct tttatcctta agaatctatg
     5041 ttccttcaga atccctgtca gaattaccgt taaaggaaat caaacataga ctctctgatg
     5101 ttaccttcaa acgaataaag aaaaagcggc gaagtatcca attcgatgtc aagaaaggtg
     5161 agcagcatct gcgtgtgcaa tttttagatt accgagaatt agaacaattg attactttac
     5221 aaaaaatatg aggtgaagaa aatggataaa caactctatt atcgaataat ggttccggca
     5281 tcattattat ccgtctgtct cttttttgtt cctaattttc ctattcgatc agtcatcggt
     5341 tttttggtca tgtttggcgg gatgggactt tattattatc ttcttcataa ggaaagaaaa
     5401 cctgcttctg tttatatgaa aaaaacaaga agcgaaaata aaaaacgata aaacggcttg
     5461 atgatagaca aaaaaataaa aaaccagt
//
LOCUS       DAXWOO010000166         5307 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.170, whole genome shotgun sequence.
ACCESSION   DAXWOO010000166 DAXWOO010000000
VERSION     DAXWOO010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.170"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(57..1196)
                     /locus_tag="ACGE70_002467"
     CDS             complement(57..1196)
                     /locus_tag="ACGE70_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFF8694071.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(1505..2218)
                     /locus_tag="ACGE70_002468"
     CDS             complement(1505..2218)
                     /locus_tag="ACGE70_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFF8694072.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(2472..3173)
                     /gene="nagB"
                     /locus_tag="ACGE70_002469"
     CDS             complement(2472..3173)
                     /gene="nagB"
                     /locus_tag="ACGE70_002469"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFF8694073.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            3407..4939
                     /locus_tag="ACGE70_002470"
                     /pseudo
     CDS             3407..4939
                     /locus_tag="ACGE70_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
     gene            complement(5130..>5307)
                     /locus_tag="ACGE70_002471"
     CDS             complement(5130..>5307)
                     /locus_tag="ACGE70_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFF8694074.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         1745 a         1079 c          892 g         1591 t
ORIGIN      
        1 ataaaaagag tttagcgtac agatatttta aatgtacact gaactctttt ttatgcttat
       61 tcacttgttt cttccagcac ttttggactg tctaactttt ccgaaacatg ttccattctt
      121 ctcaataaat agattttccg gatatttaat attatcgtct taatcactgc atagacaggt
      181 atgcaaatca gcatgcctaa tattcctgcg acacttcctg ctccaatcaa taccaagatg
      241 atcgtcaaag gatggatatt cattgatttc ccaaataaca acggcttgat caaatttcca
      301 tctaattgtt ggatcactaa aatcgacaac gccatataca aagcttgaaa tggagagata
      361 aacaaaccga caatgattgc tggtaccgct ccaataaaag ggccgacata cgggataata
      421 ttcgtgattc cgcaaatgac cgcaagcaac aagctataag gctgatcgaa tattctcatt
      481 gccaaaaagc tcattacacc aataatcaaa gcatctaaga tcgtactgct gatataggca
      541 gataatgttt cgtttaattc ttttaccgtt tggcgtaact ctttacgaat cgctttcgga
      601 aagaagtgag acaacgcatc aataaatttg tgcccatcct tgaacataaa tagcaaaata
      661 aatggaacgg taaataacaa aataaaaaat tgcatcataa atgaaatgat ttttgagaca
      721 cttgcagata aactgactaa tatgatattg aacaaattac taagagataa atttgctgat
      781 gctaattgtt cttccaaatt gaaattttta aactcttccc gctgcgccaa ttcatttaac
      841 catttgcttg tttcatctgc atacattggc aagctctggg tcaattgaat tgtctgttcc
      901 attaactgag gcactacatt catgacagtt aaaacaacta aaatgattac gaccaaaaac
      961 gtcaataaaa aaccgaacac tctcgggatc cgatgctttt cgagaaaaac aacgattgga
     1021 tcgaacatat aatagataaa accagcaaca agcaatggta aaaaaattgc cgaaagcatt
     1081 ttcaatattg gactaaaaat attcggcatt tgtaaaataa agtacacgcc gatgatcgtg
     1141 agtactagct ccactgtcca gaaaaataat tttgattgtt taaaacgtga ttgcatacat
     1201 ctcctccaag gtcctttgat acttctattc tatcatttaa caaagagtta cgcgctgaga
     1261 ttttcatttg cttaaaaaaa acaaaaaaaa taaacatatt ttaatgagta gacgagtact
     1321 taaacaaaaa cacggtttgg agtagtgaca aaaattatac tgcagtccct ttttccaaat
     1381 aaacggtgtt caaaagcttg catcttcgta ataagcccag ccaaacgaaa aatataagga
     1441 actattttta tttggctggg cttattactt gatgtaggac atctatttta caacctcaga
     1501 tcatttattg cgcaatactg actaaatgca actttgtgat atttttgtgt acggacaact
     1561 cctcgatcac atcggcataa gtgagccctt ttggaagatc aatcgtatag atatttttgt
     1621 acactcggta gtcatcaacg aattttacgt caaaattcac atcttcgatt acgatatttt
     1681 tctcttcaaa ataatcggta agaaaatctt tggtttcttt tctatggata aattggattt
     1741 ctaacttttt agtcgtaggt acatggatca cgcgtttcac tactgtgagt gcaatagcaa
     1801 tcccgataaa actgatgatc gcaatggaat agtaacccat gccgatggcg atccctaatc
     1861 cagcaattgc ccataaagaa gcggcagtcg taagtcccgt caccgattgt ttcgtgacga
     1921 tgatcgttcc cgcgcctaag aaaccgacac cactcaccac ttgagcaatc agccttgctt
     1981 catctgaacg aatcgttcca gcaagctgtg gatactctaa agcatctctt aatgcaccac
     2041 tagcgatctc aacttggatc aatgcaatga tacatgctcc catacaaact aaaatatgcg
     2101 tacgcatacc tgctggacga tttttatatt gtcgttcaaa accgattgct ccgccaaata
     2161 gcatagcgat ccctaaacgt aaaataatct ctggaattgt caataccata tcattcatct
     2221 aaacaccctc cagtccaata tatcatgtct gaaaggagca gacaaaaaaa gtgcaatttt
     2281 tacctcttac gaaaaaagga ctgtgacaaa atttttgtca tagtcctttt tatgaataaa
     2341 cggtgttcaa tcagctgacc ctcggtatta ctcaacaaac ggcaaaatat ggggagctat
     2401 ttttcctttg tcttctctaa tactcaggtc aaaacgctga tttcacaacc tctttagcct
     2461 actattgaaa attacaactt acttgctgct tcttcatcga tgatcacaac aacattgtcg
     2521 tgattttgca atacacttgc tggcaaatga ttcgttacag gtccttcaat catccctttg
     2581 attgcatcag ctttgctttc gccaaatgcc atcaagacga tttctttact tttcataatt
     2641 gaaccgattc ccattgaaac agcttttgtt ggtacatctt ctgctttatc gaagaatcgt
     2701 ttgtttgcat tgattgttga ttctgttaag tcaactactg aagtcgttcc gtcaaatgaa
     2761 gcaccaggtt cattgaaacc gatatgtcca ttttgcccga ttcctaagat ttggatatcg
     2821 attggatgtg catcgatgat ttgatcatac tttttgcatg ctgcttccag atcttctgct
     2881 tttccatctg gtacaaacgt ttctttgaat ggttttttat caaataattg gacatccatg
     2941 aaatgacggt agctttgttc atctgaaccg cctagtccaa cgtactcatc taagtttaca
     3001 gaagtcattt cactgaaatc caaatcgctt gaagtcattt ctttatataa agtaactggt
     3061 gtgcttcctg ttgccaatcc taatactttt gcacctttgt tcataccttc tttgatgatt
     3121 tcaaatgctt tttttccgcc ttcttcagcg ttttttacac gaatgatttc catagtgatt
     3181 gctcctttat attttggtat agcccaatta tagcattctt tttttctttt tacaataatt
     3241 agtatagacc aaattgcatt tttttcattt tcttgacaat aaataggaat ccgtgttagg
     3301 tttcataaga tggttcaatc aaacaatcca tgttgcatga aaaccaagca agtgattcac
     3361 tgtggacgct ttatgtctgc agttttttta tgaaaaagga gttattatga gtatactgac
     3421 attaaacaac attacccagc aatttggcga caaaatcctc tatgaaggtg tcgatctgca
     3481 attaaatgct ggcgaacatc ttgggctgat tggacaaaac ggagccggta aaagtacatt
     3541 gattaaaatc atcactggtg agatcttacc agatgacgga cagatccatt ggcaaaaaaa
     3601 tattcacaaa ggttacctcg atcaatatgt ggaagtcgat gaaacattaa caatcgaaga
     3661 atttctaaaa actgcgtacg caaaagaata cgaaaaggaa gaaaaaattg cttttcttta
     3721 ccaaacatat agcgagacct tcgatgattc cttgctggaa aaagctggga aactccaaac
     3781 ggaattagac caaggagtgt tttatcaaat cgacacctta gttgctgaga tgagcagcgg
     3841 gttggggatc gatgtcttag gtttagattc actgttgaaa aatctaagtg gtggacaacg
     3901 ttcaaaggtg attttagcaa aactactttt agaaaaccca gatgttctga tcttagatga
     3961 accaaccaat cacttagatg atcagcatat ccaatggctg acgcagtttc ttcaggattt
     4021 tgacggtgcg tatatcgtga tttcccatga ccaagaattt cttgatcaga tcaccaccca
     4081 tatcgcagac atcgagtttg gaaagattac taagtatact ggccatctaa aacaagcatt
     4141 gaaacaaaag gaacaaaatc gcgaaagcta tttgcgccaa tatcatgctc aacaaaaaca
     4201 aatcgaaaag accgaagctt atattcgaaa atacaaagcc ggttcccgat caacgatggc
     4261 aaaaagccga caaaaacaat tagataaaat cgaacggctg acgcctccag cttcagcaag
     4321 taaagctgtc tttgattttc cattctcgcc tattgtcacg acattagcag tggaaacaac
     4381 tgatttagtc atcggatatg atcgaccgct acttgcaccc atcaatttga ccatccgctt
     4441 tggtgaaaat attgccattc gaggcttcaa cgggattgga aaatctactt tattaaaaac
     4501 actgatcggt gagattgaaa aaataagtgg cgatttccac ttcccagata acacaaaaat
     4561 caattacttt tcacaagatc tgtactggga aaatccatta gaaacaccac tacaatattt
     4621 aagcagttaa tttccaaaag cgactatcaa agaattacgt cgccaactag ctaaagcagg
     4681 gttagttaat caattagcaa gtgaacctct cactacatta agtggtgggg aacaaactaa
     4741 ggtgaaactt gcacagttga caatgaacca aggtaattta ctgattctcg atgaaccgac
     4801 gaaccatatc gatcaagaaa cgaaagaaag cttacaagac gggatacaga aatttccagg
     4861 aacagtaatc atcgtctctc atgaacaaga attttatcaa gatcttgtgc aacgagtcat
     4921 tgagattgaa ccaaaataaa cgtttgattg aatagttgaa aaaaaggagc tgtgacaaaa
     4981 gttttgtcac aactccttct tctgaataaa tggtggccaa ttagctgacc ttcggtatta
     5041 gctcaaaaaa cggcaaaata tgaggagcta tttttatttg gctattctta ttacttgatg
     5101 cagaacagct tttcacaacc tcttttcgtc tattttttat tttcgagatt tctctttcct
     5161 ttaaaaacaa ctgtttgaaa gaagaacgca catccttgaa aaacaagccc gctaaccgtt
     5221 acacctaacc agccatactg ctgccacatt gtcgtcccta gcaatgaacc agccgcacct
     5281 ccaataaagt acataaacat aaataca
//
LOCUS       DAXWOO010000167         5115 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.171, whole genome shotgun sequence.
ACCESSION   DAXWOO010000167 DAXWOO010000000
VERSION     DAXWOO010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5115
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.171"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            44..1333
                     /locus_tag="ACGE70_002472"
     CDS             44..1333
                     /locus_tag="ACGE70_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFF8694075.1"
                     /translation="MRYSTILISTFAALSLSLTINQTVHTEEIETAKWTETKWANVNR
                     ITFQDEFSSFNSGVAKLRKGQTATIKAKIDYSGDLAPILQSAVTFENVDPALSIGYDE
                     NTLTIQHKQAIFTFTVTLNQDLLKPALFTVKVSDGLPNDHHHLTPYSQRQTIEQDSAI
                     DSGGDLVEEPTDKPENENKPEVPPTENPDGEQKPEIEPGEEPDTETQPEPDNESKPEI
                     TPGEKPDVDPEEKPDVTPEPDTDSGNQTVPETNPDTDNETENPEKPEVAPEEKPDVTP
                     EPDTDSGNQTVPETNPDTDNETENPEKLEVDPEEKPDVTPEPDTDARDQGIPEKINKK
                     TIQEDGKKESKKSNLAILKINEEQLNKKSRIFDSAQSAETLKSSKDTTFASPETKNKQ
                     LPKSGESQNKVILWSGIILLSIATMLSAKRFKQNRSL"
     gene            complement(1459..2538)
                     /locus_tag="ACGE70_002473"
     CDS             complement(1459..2538)
                     /locus_tag="ACGE70_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFF8694076.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVIALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(2647..3087)
                     /locus_tag="ACGE70_002474"
     CDS             complement(2647..3087)
                     /locus_tag="ACGE70_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFF8694077.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(3144..3554)
                     /locus_tag="ACGE70_002475"
     CDS             complement(3144..3554)
                     /locus_tag="ACGE70_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFF8694078.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(3735..4169)
                     /locus_tag="ACGE70_002476"
     CDS             complement(3735..4169)
                     /locus_tag="ACGE70_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFF8694079.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(4269..>5115)
                     /locus_tag="ACGE70_002477"
     CDS             complement(4269..>5115)
                     /locus_tag="ACGE70_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFF8694080.1"
                     /translation="KETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGIL
                     LEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITSD
                     RLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVSL
                     NLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIGR
                     GLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGFT
                     YHPIE"
BASE COUNT         1881 a          988 c          871 g         1375 t
ORIGIN      
        1 atcttatttc ttgcatacaa aaaaatcaag gagtgagaga gttatgagat attcaaccat
       61 cttaatatca acatttgcag ccttaagctt atcgcttact ataaatcaga cagtccacac
      121 agaggagata gaaacagcca aatggacaga aaccaagtgg gccaacgtca atcgaattac
      181 ctttcaggat gaattttcat ccttcaattc aggagtcgca aaattaagaa aaggacaaac
      241 agcgaccatc aaagcaaaaa ttgactattc aggagactta gcacctatac tacaatcagc
      301 tgttacattt gagaatgttg atcccgcttt gtcgattggc tatgacgaga acactttgac
      361 tatccaacac aagcaagcga tatttacctt caccgttaca ttaaatcagg acttgttaaa
      421 accagcgctt tttactgtca aggtatcaga tggactaccg aacgatcatc atcatttaac
      481 gccgtatagt cagcggcaga caattgagca agattcagct atcgactcag gaggagatct
      541 tgttgaagaa ccaacagata aaccagaaaa tgaaaataag ccagaagtac cgcctacaga
      601 aaatccagac ggagaacaga aaccggaaat agaacctgga gaagaacccg atacagaaac
      661 tcaaccagag ccagacaatg aatcaaaacc tgaaataacc cctggagaaa aaccagatgt
      721 agacccagaa gaaaaaccgg atgtgactcc agaaccagat acggattctg gaaaccaaac
      781 agtaccggaa acaaatccag acacagataa tgaaactgaa aatccagaaa aaccagaggt
      841 agccccagaa gaaaaaccag atgtaactcc agaaccagat acggattctg gaaaccaaac
      901 agtaccggaa acaaatccag acacagataa tgaaactgaa aatccagaaa aactagaggt
      961 agatccagaa gaaaaaccag atgtgactcc agaaccagat acggacgcaa gagaccaagg
     1021 aataccagaa aaaataaaca aaaaaacaat tcaagaggat ggaaaaaaag agagtaaaaa
     1081 atcaaatcta gcaattctaa aaataaatga agagcagctg aataaaaaat cacgaatatt
     1141 tgatagcgcc caatcagctg aaaccttaaa aagtagcaag gatacaacct ttgctagtcc
     1201 tgaaacgaaa aataaacagc taccaaaatc cggagagtca caaaacaaag ttatactgtg
     1261 gagcggaatc attctattaa gtatagcaac aatgctttct gcaaaaagat ttaaacaaaa
     1321 cagatctctc taacatttga ataatgtctg tgaacgcatt tttcaaataa aaagaaatat
     1381 ccagaaaacg accccaaaat accagattga aatctgatat ttggggtcgt tcatacttta
     1441 tctttcttca tatttatttt aagggatatc tgtttctttt ggaagatggt tcgggatctt
     1501 tttcactttc aatacatcta aaaagaaggt aaagatcgga atgccgacaa tcaagcccca
     1561 aacgccaaag aaccgttcgc taacaagtaa gaccacaaat gtataaaaaa tcggcagttc
     1621 tgttttgctg gacatcaact tcggattcag cacataagac tcaattagat ggacaatcaa
     1681 aatcgtaatc aggatataaa caacatctcg gatgcctcct tgggaataag ctatgaaaga
     1741 tagaggaata caagaaatga taacacctgc tactgggatc agacttaaaa tgaaaatcat
     1801 gatcgctaaa ctcagcagct gatgaaagcc aaatacagcc aaggcgattg tagtgagcaa
     1861 cgtgtttaaa agagcgataa caaattgagc ttctaatacc aaaccaaatg tattgacaaa
     1921 cttatcggca aaatagtaaa tatcttgaaa gaaccaagaa aattcacttt ttaaaaacaa
     1981 tcttgagaat acaattgttt ttttcttttc aatcataaaa aagaaactta agatgaatga
     2041 catagcaaac gccactgcca tttttcctat atcctgtaaa tagccaagcg caatcgaagc
     2101 gccattttga agctgttcaa aaaaatcgtt tcgctcaata taagtatgga tatattgtaa
     2161 aacaggatct gcatcttttg gctgatgctc gtaaaaatca ataacggact ggatcatatc
     2221 aaatgtttga tgaataagca tcgggatata ctttgtgaat gccagataga taaggtaaac
     2281 gatcagtgta taaatcatga tagctaaaac acctgtaggg attcggacaa atcgctgaat
     2341 gatttgaaca agatgaatca tcaaataagt aaagataaat gtgagcaaaa tggttgtaat
     2401 catacttcta actaagaaaa gaacaaaaac gacaaataat aaaacagtga gacggcgtat
     2461 tttttcattc gccaaaaaac gctgataaac ggtcaaatcg acaacctcca aataacaaaa
     2521 aataaacacg ttattcatta tatgatggaa tatgaaaata agaaagagaa agagcaaaaa
     2581 gaaaaaccgc taaatgacgt tatcttatga gttggttttc ctcataaaat cgttcatcta
     2641 gcagtttcag ccctttttct ggtcttgatg aaaattatct agcatttttc gggtcagatg
     2701 ttgcagtgta tccaattctt cctgttcaag tggaagataa gacttgatct tttgtggaat
     2761 accttctgct tgtttttgca acgcagcacc ttgaggagtc agcgtaacat ttacgattct
     2821 ttcatcaccc gtttcacgct gacgattaat ataatttttt tctgctaact tttttagtaa
     2881 gggagtcagc gtaccagaat ccagacacaa atcatttcct agctctttca ctgaagtctt
     2941 tttcttttcc cataaaacta acatcacaag atactgggta taggtaagat tcagcggctt
     3001 gaggagggaa gtgtagtgtc taatgatttc cttagaggcg gcatataaag gaaaacatac
     3061 ttgattaact aaatatagat cttccatttt tccacctccg ctgttttact atagtgtatc
     3121 atagcggatt tacacaaaaa agtctattca ctaactgctt tcagagtaac ttctatattt
     3181 ccagctgtag cttttgaata cggacacact ttatgcgctg cctctaataa ttcttgcgtt
     3241 tcttccagag aaagaccttc gatagatcct tcaatctcta cacctaagac aaagctcgtt
     3301 tcactttctg aataaaggga aacttgtgca ctgacagtag aagccccaga gatatttttt
     3361 tgctgtttga tcaattctaa tgcactgtta aaacatgcac tgaaaccagc tgcaaacaat
     3421 tgttcagggt tggttgcatt ttcgacgcgt ttaccaggag ctgcaatatt caattcgaat
     3481 gtacgatcgg gattatgaac ttctccagag cgtccgcctg tgttgatcat tttcgtagaa
     3541 tacatttttt tcataaaatt accaccttta attatatttt atacaattga attttacaca
     3601 atataattgt ggaaagcaaa gaaatggatt ggcataagat aaataagaga aagcacatga
     3661 aacagacgcg ctttctcgaa tagcaaacat atacaaaaaa atatcagaga caaaaactaa
     3721 tctcatcata aacattacat atatttctca aaaagttgtt ttctttcttc aaaacttact
     3781 tcacgatgga cctgatgaag catccataaa taaccaaaag gatctctgaa cattgagtta
     3841 gtcacaccaa agtccatcat ttctgtcatt tcttgaatct ctacacagcc atttcgtaat
     3901 gctttattat gtgtgtcctc gatatcagag acgatgatat tgaaccagat cgtatttgga
     3961 tgttcagcag taggagcagt caaaccaaga gacgtgtttt catccagcat atggaatctc
     4021 gtgccataca aggtaaaaat gacttcatta ttgcctctag ggtaatctgt tgcctcaatc
     4081 agttgtattt catcaaagat tgtttgatac agttctagtg ctttcaaact atcatttaat
     4141 acaaaatcta tttccgttcc tttatgcata aagatctcct ctccttttca gtcgaacagt
     4201 tactagattc ttttattata ccagttaaag aaaacgcatt caaaaaaata gcggatttac
     4261 ttaccaactc attcgattgg atgataagta aatccgcgtc ccattacttc tgctacgctg
     4321 ctgacaatga caaaggcact tggatcgatt tcttctatca atggtttcat tcgggaaaat
     4381 tcatgatcag tcacaacgat caataaaacc tcttttgact ttcgattata cccgcctttt
     4441 gcctctagca gggtaaggcc acgtccaatc tcagcactta agcgaaggcg gatgtcttcc
     4501 aaataatgtt cactcatgac cataacagat ttctttcgat tgatacctgt ttcgatataa
     4561 gtcatgacaa ttgaagtgat tgcaatagat aaaatagcat aaagaaactc ttctattcca
     4621 aaaacaaaca gattaagaga aacaacgaat gcgtctgtaa aaaataagcc aatcgaagta
     4681 ttcaagtgga aatatttttt aaaaatgagg ggagggatcg tcgtaccgcc gctagaagag
     4741 ttgtttttat ataaaatcgc aactcctaaa gcaaaaatcg cacttccaaa aatgacagac
     4801 aacaaccgat ctgaagtaat cattgtttct ggaactattg caatgattaa aggaaaaaac
     4861 aagcttccga aaagtacttt aaaaaagggt tttttaccta aaaatagaag agctagtacc
     4921 aacataacga tattcaacac taaaataact atagctcggt caaatcctaa aacagcttcc
     4981 agtaatattc ctagaccgct gacacctcca gcagcaatat gatgaggccc taaaaacata
     5041 ttgatagcga ctcctaatat gaagagggca aaaataatct taatgtatgt ccatataaat
     5101 tgtctcgttt ctttc
//
LOCUS       DAXWOO010000168         5080 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.172, whole genome shotgun sequence.
ACCESSION   DAXWOO010000168 DAXWOO010000000
VERSION     DAXWOO010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.172"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            206..1480
                     /locus_tag="ACGE70_002478"
     CDS             206..1480
                     /locus_tag="ACGE70_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFF8694081.1"
                     /translation="MSKVNVFKNQSYLQSSATLLLFFASWGVWWSFFQLWLTSESNGL
                     GLSGSAVGTVFSANSLVTLILMFIYGTLQDKLYIKRNLLIFASVLATLVGPFFIWIYG
                     PLLDNNFNLGIIMGALFLSAGYLASVGVFEAVSERFSRLFGFEYGQARAWGSFGYALV
                     ALLAGFLFVKNPHLNFWAGSFFGSLLLLNLLFWNPKVEREANQNFNQEQAESNSIPSL
                     KEMFDLMKLPQLWTIIIFIVFTWTFYTVFDQQMFPGFYTGLFSTSANGEKIYGTLNAI
                     QVFCEALMMGIVPIIMRKLGVRNTLLLGVTIMCVRIGLCGFASTPLSVSCIKMLHALE
                     VPLFTLPMFRYFTLHFDTKLSATLYMIGFQIAAQIGQVILSTPLGILRDNVGYQPTFK
                     IISLIVLLAGIYAFFILKQDDRDVQGDPFIRG"
     gene            1534..2961
                     /locus_tag="ACGE70_002479"
     CDS             1534..2961
                     /locus_tag="ACGE70_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFF8694082.1"
                     /translation="MTKRAIELTNTRYRLGYHVSAPSGWINDPNGFCYFDGYYHVFYQ
                     HHPYSAEWGPMHWAHARSKDLVHWESLPLALTPGDQEDEGGCFSGSAIEKNGVLYLFY
                     TGHHYYGDNDPDHFWQNQNMAYSTDGIHFTKYEKNPVIAKAPEDNTHHFRDPKVWKHD
                     EKYYMVLGSQEKDGLGRAIVYQSLDLLNWDYIGPISKAKEVLTEGFMWECPDFFELNG
                     KSILLLSPQGIEANGKDYLNLYETGYFIGDYDYETAKFIRGNFYELDKGHDFYATQTT
                     LSPDGRRIVMAWMDMWESPMPEKVDGWAGALTLPRELELIGDRLYMKPVRELEQLRVG
                     SGVETTVNLAGEIIIAQDASFKEALIDVLLTKSKNQEVKFSLKTKDEELINIFYSKDK
                     NECTLTRNGKNDPRYGTIQMCDRLSLRVFIDKSSAEIFINDGELVFTERYYTENQPSM
                     HLSVSEEAFVSSTIYHLKKDAISYE"
     gene            2974..3846
                     /locus_tag="ACGE70_002480"
     CDS             2974..3846
                     /locus_tag="ACGE70_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFF8694083.1"
                     /translation="MLIGSIEAGGTKFVCAVGDEDYRVQDQIICPTTTPRETLKKTVD
                     YFKQFDINALGIASFGPIEIRKNSPKSGYITSTPKVGWRNVNVLGYLQDELNVPMSWT
                     TDVNGSAYGEYIMSTLSNEKINSLVYYTVGTGVGGGAVVDGKLLGSFGHSEMGHTFVK
                     RHPDDIDFKGICPYHGDCLEGLVAGPTFEARFGKKGEDVSLTDPIWDVMAYYLAQAVI
                     QTTLIIRPDKIVFGGSVVNEKLLEKIRVNVNKLLDDYVEIPPLSKYITRPLIKNNGSA
                     TLGNFALGLNQLQE"
     gene            3941..4936
                     /locus_tag="ACGE70_002481"
     CDS             3941..4936
                     /locus_tag="ACGE70_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8694084.1"
                     /translation="MRPKLEDVAKRANVSKTTVSRVLNNRGYLSQKTIDNVYKAIEEL
                     NYQPNVVARQLFQKKTNIVGLLFPTVANPFFSELVEALEKKLYEIGYKVLIGNSMNNK
                     EKETNYLNQLLSDQVDGLIVGTHNQGIQEYKYQNLPIVAIDRVMNEDIPVVESDNYNG
                     GKLATKLLIAQGAKNIIHTNGPIDLQTPANRRRLAYEDTMKAYQLIPRTVTLDFNISY
                     VKKKQIFFQMFEDYPKIDGIFASNDIDAALILQVAKEKGLNVPADLLVVGYDGTLMTR
                     SILPDLTTVIQPINDIADTAVAILMKRINKEETKKEYILPVTLWMGKTSVKKKNN"
BASE COUNT         1753 a          699 c          991 g         1637 t
ORIGIN      
        1 tataaaaaat agtgccatta aaaaagaaaa gcgacacacc aataaaagtc atatcctgtg
       61 acattatatg gcattatcca aatagaataa ccggttgaca tgtaagcgga tttcttttat
      121 aattttagga gtacaagtcc ttttctaaaa atgttaaccg gttatcatga aaggggatac
      181 aaatatatcg ttggaggata aaaatatgtc aaaagttaac gtatttaaaa atcaatctta
      241 tttacaaagt tcagctacat tattactatt ttttgcttct tggggtgttt ggtggtcatt
      301 ttttcaactt tggctaacat ctgaatcaaa tggtttaggg ttatctggca gtgctgtagg
      361 aacagtattc tcggcaaatt cgttagttac cttaattttg atgtttattt atggaacatt
      421 acaagataaa ttgtatatta aacgaaattt attaattttt gcttctgtat tagcgacact
      481 tgttggacca ttttttatat ggatatatgg gccattgcta gataacaatt ttaatttagg
      541 cattattatg ggagcgctat ttttgtcagc tggatattta gcttctgtag gagtttttga
      601 agctgtgtca gaaaggttta gtcgtttatt tggctttgaa tatggacaag caagggcgtg
      661 gggatcattt ggttatgcct tggtagcgct tttggcagga tttttatttg taaaaaatcc
      721 tcatttaaac ttttgggcgg gatctttctt tggttcttta ctattgttaa atttattatt
      781 ttggaaccct aaagttgaac gggaagcaaa tcaaaatttt aatcaagaac aagctgaatc
      841 aaatagtatt ccttctttaa aagaaatgtt tgatctaatg aaactgcctc aattatggac
      901 gataatcatc tttattgttt ttacatggac attttatacg gtattcgatc aacaaatgtt
      961 tccgggattt tatactggtt tgttttcaac atcagctaat ggtgaaaaaa tatatgggac
     1021 attgaatgct attcaagtat tttgtgaagc gttaatgatg ggaattgttc caatcattat
     1081 gagaaaatta ggggttcgaa atactttgtt attaggtgta accattatgt gtgtacgaat
     1141 tggattgtgc gggtttgcct cgacaccatt atctgtttca tgcataaaaa tgttgcatgc
     1201 tttagaagta ccattattta cattaccaat gtttcgctat tttacacttc attttgatac
     1261 aaagctatca gcaaccctct atatgatagg atttcagata gctgctcaaa ttgggcaagt
     1321 gattttatca acaccattgg gaatattaag agacaacgtt ggctatcaac caacatttaa
     1381 aattatttct cttattgtat tactagcagg catatatgca ttctttattc ttaaacaaga
     1441 tgatagagat gttcaagggg atccatttat tcgaggataa tggaaaccaa cagaataaaa
     1501 aacaatttta tctagaaagt gagcgaatat attatgacaa aaagagctat agaattaaca
     1561 aatacacgtt atcgtttggg atatcatgtg tctgcaccat caggatggat taatgatcca
     1621 aatggatttt gctattttga tgggtattat catgtgtttt atcaacatca tccatatagt
     1681 gctgaatggg gaccaatgca ctgggcacat gctagaagta aggatttagt tcattgggaa
     1741 tctttaccac ttgctttgac tcctggagat caagaagatg aaggtggttg tttttcagga
     1801 agcgctattg aaaaaaatgg tgtgttgtat ctgttctata caggacatca ctattatgga
     1861 gacaatgatc cagatcattt ttggcaaaat caaaacatgg cgtatagtac ggatggcatt
     1921 cactttacta aatatgaaaa aaatcctgta atagcaaaag caccagaaga taacacacat
     1981 catttcagag atccaaaagt atggaaacac gatgaaaaat attacatggt tttgggtagt
     2041 caagaaaaag atggattagg tcgtgctatt gtttatcagt cgttagattt attaaattgg
     2101 gattacattg gaccaatttc taaagcgaag gaagtactta cagaaggatt tatgtgggaa
     2161 tgcccagact tttttgaatt aaatggaaaa tctattcttt tattatcccc tcagggaatt
     2221 gaagcaaatg gaaaagatta tttaaatcta tatgaaacag gctactttat cggtgattat
     2281 gattatgaga cagcaaaatt cattagagga aatttttatg aattagataa gggacacgat
     2341 ttctatgcaa cacaaacaac tttatctcca gatggtagac gtattgttat ggcctggatg
     2401 gatatgtggg aaagccctat gcctgaaaaa gtagacggat gggcaggagc attaacgctt
     2461 cctcgagaat tagagttaat tggagatcgt ttgtatatga aacctgtgag agaattagaa
     2521 caacttcgag ttggcagcgg cgttgaaacg acagttaatt tggcaggaga aataataatc
     2581 gctcaagatg cttcattcaa agaagctcta attgatgtac tattgactaa atcaaaaaat
     2641 caagaagtca aattttcatt aaaaacaaaa gatgaagagt taatcaatat tttttattct
     2701 aaggataaaa atgaatgtac cttaactcgt aacggcaaaa atgatccaag gtatggaacc
     2761 attcagatgt gtgatagatt atcacttcga gtgtttatcg ataaaagttc ggcagagatt
     2821 tttattaatg atggcgagct tgtttttaca gagcgttatt atacagaaaa tcaaccaagt
     2881 atgcatttga gtgttagtga agaagctttt gtctcgagta caatttatca tctaaaaaaa
     2941 gatgcaattt cttacgagta aggaggtttt atgatgttaa ttggtagcat agaagcaggt
     3001 gggactaaat ttgtatgtgc cgtgggagac gaggattacc gagtacaaga ccaaataatt
     3061 tgtccaacga ccacccccag ggaaacattg aaaaaaactg ttgactattt taaacagttt
     3121 gatataaatg cattaggtat tgcatcattt ggtccaatag aaattcgaaa aaattcacca
     3181 aaatcaggat atattacgtc tacaccaaag gtgggatggc gaaatgttaa tgtgttaggt
     3241 tatttacaag atgagctgaa tgttccaatg tcttggacaa cggatgtgaa cggatcagct
     3301 tatggagagt acatcatgtc cactttatcg aatgaaaaaa taaattcatt agtttattac
     3361 accgttggta ctggcgttgg tggtggagct gttgttgacg gaaaactatt aggatcgttt
     3421 ggtcattcag aaatgggcca tacgtttgta aaacgccatc ctgatgatat tgattttaaa
     3481 ggaatttgtc cttatcatgg tgattgttta gaaggcttag ttgcgggacc aacgtttgaa
     3541 gcacgttttg gaaaaaaggg tgaagacgtt tctttaacgg atcctatttg ggatgtaatg
     3601 gcctattatc tagcacaagc tgtgattcaa acaacattga ttatacgacc ggataaaata
     3661 gtatttggtg gaagcgtagt aaatgaaaaa ttgttagaaa aaattcgtgt taatgttaat
     3721 aaattattgg atgattacgt ggaaatacca ccgttaagta aatatattac gcgtccttta
     3781 attaaaaata atggatcggc aacactagga aactttgctt tgggcttgaa tcaattacaa
     3841 gaataaaaca tagaaaaaat atcgattatc aggtatagta gttctataga gttctttttt
     3901 tcgatagtta attattgcat tatttaggag agacaagaga atgcgcccaa aattagaaga
     3961 tgttgccaaa agagcaaatg tatcaaaaac aactgtttca cgtgtattaa acaatcgtgg
     4021 ctatttaagt caaaaaacga ttgataatgt ttataaggct atagaagaac tcaattatca
     4081 accgaacgtt gtagcgcgtc aattatttca aaagaaaact aacattgtcg ggttactatt
     4141 tcctacagta gccaatcctt tttttagtga actcgttgag gcattggaaa aaaagttata
     4201 tgaaattggt tataaggtat taataggcaa ttcgatgaat aataaagaaa aggaaacaaa
     4261 ttatttaaat cagttattgt ctgatcaagt agatggttta attgttggta cacataacca
     4321 aggaattcag gaatataaat atcagaattt accgattgtt gcaattgatc gagtgatgaa
     4381 tgaagatatt ccagttgttg aatccgataa ctacaatggt ggaaaacttg caaccaagtt
     4441 attaattgca caaggtgcaa aaaatattat tcatacaaac ggtccaattg acttacagac
     4501 tccggctaat cgtcggagac tagcctatga agatactatg aaagcatatc agttaattcc
     4561 acgaactgtg acattagatt tcaatatttc ttatgtaaag aaaaaacaga tttttttcca
     4621 aatgtttgag gattatccta aaatagatgg tatttttgct tcaaatgaca tcgatgcggc
     4681 cctaatatta caagtagcaa aagaaaaggg gttaaatgta ccagctgatt tattagtagt
     4741 cggatatgat ggtactttaa tgactcggag tatattacca gatctgacaa cagtaataca
     4801 accaattaat gatatagcag acacagctgt tgctattttg atgaaacgaa taaataaaga
     4861 agaaacaaaa aaggaatata ttttacctgt cacattatgg atggggaaga catcagtaaa
     4921 gaaaaagaac aattaaattt ataaagacta tcaatgataa tttcattatt aattcgtgat
     4981 ataaaaaatt gttaaatctc ttataataga ctatgataca aaatcatgct tttaatctgt
     5041 gtagatgttg ccaaatattt gaaagacata gaacttgtca
//
LOCUS       DAXWOO010000169         5023 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.173, whole genome shotgun sequence.
ACCESSION   DAXWOO010000169 DAXWOO010000000
VERSION     DAXWOO010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5023
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.173"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(157..1206)
                     /gene="recA"
                     /locus_tag="ACGE70_002482"
     CDS             complement(157..1206)
                     /gene="recA"
                     /locus_tag="ACGE70_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFF8694085.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(1315..2556)
                     /locus_tag="ACGE70_002483"
     CDS             complement(1315..2556)
                     /locus_tag="ACGE70_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFF8694086.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(2796..3374)
                     /gene="pgsA"
                     /locus_tag="ACGE70_002484"
     CDS             complement(2796..3374)
                     /gene="pgsA"
                     /locus_tag="ACGE70_002484"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFF8694087.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(3583..4506)
                     /locus_tag="ACGE70_002485"
     CDS             complement(3583..4506)
                     /locus_tag="ACGE70_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFF8694088.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
BASE COUNT         1360 a         1132 c          901 g         1630 t
ORIGIN      
        1 ttctattaaa ttattctttg tagatgaaca gatattttta ttttatgtac ccactcgtat
       61 aattaaataa ctcacgattt ctttattcaa taaaaaaacg gcaggtcaag gagagtacct
      121 actcttcaga ctagccgttt ttgtttatca taatttttat tcttctacta aaggcaattc
      181 ttcttgtcct ttttcatctt ctgggacagc gacttcttca ccaataccat aagctgctct
      241 aactagagca gaaacttctg ccatcatttc aggatgatta gccatataga ttttagcgtt
      301 ttcccgtcct tgaccaatcc gatcttcttt ataagaatac caagcaccgc ttttatcaac
      361 aatgtctttt tctactgcca tgtctagaag ttctccttct tgtgagatcc ctaaaccata
      421 catgacatct acttcagcga ttttgaaagg aggtgctact ttgtttttta cgactttgat
      481 ctttgtacgg ttaccaacaa tatctgtacc ttgtttcaat tgttctgcac gacgtacttc
      541 caaacggatc gttgcgtaga atttcaatgc gcgtcctcca ggagtgattt ctggattccc
      601 aaacatcacg cctacttttt cacggatttg gttgatgaaa atagcgattg tttttgtttt
      661 gttgatcgaa ccagagagtt tacgcaatgc ttgagacatc aaacgtgctt gtaacccgac
      721 atgtgagtca cccatttcgc cgtcgatttc tgctcgtgga actaaagcag caactgaatc
      781 gactactacg atatctacag ccccacttga tactaaagca tcagcgatct ctagaccttg
      841 ttctcctgtg tcaggctgtg aaagaagtag ttcatcgata ttcacaccta atttttgtgc
      901 atattgcgga tctaacgcat gctcagcatc aatgaaagcg gccgttccgc catttttttg
      961 tacttctgca atagcgtgta gtgcaactgt tgttttacct gaactttcag gtccatatac
     1021 ttcgatgata cgtccacggg ggtaaccgcc gacacctaaa gcgacatcta acgcaagaga
     1081 gccacttgga attgtagaga tttgttggtc gattttttcg cccaatttca taatagagcc
     1141 tttaccatag ctcttttcaa tttttttcag tgcagcatct aaggctgctt tacgatcatc
     1201 tgccaatacg ataatcctcc ttatatttac aaatcagagc aacatttcat tatccttatc
     1261 atatcttttt tagtgaaaaa aagcaagaaa aaaccgaaca aatattcgca tttatcattt
     1321 tcttaaaagc tgttttctca aaagatcaaa tccttgcata accgcattat atctgatagc
     1381 agcacgatct ctggcaaagt gatatttttc cgaaaccacg ccgtctttag atgaaatagc
     1441 gatccatact gttcctgcag attgaccttc taacgtgtcc ggtccagcca ctccggtgaa
     1501 agctacactg taatctgttc ttgctatctc acgtgcttga atggccatct gctccacaca
     1561 ttctttgctg actgttccat attctgctaa tagctccgga tcaatctcca gaaaaccagc
     1621 ttttgtttgg agcgagtagg taacgaatcc gcctggaaat acttcagata caccagcaat
     1681 gtcgcctaat gcggcttgga aggcgccggc tgtcaaactt tccgctgctg ttacagtctg
     1741 cttgttttct ttcagtagtt ccactacgac ttttgctagc gaattatcat cgccgtatcc
     1801 ataaaaatat tcccctactc gttcttggat tttttcttct aatgctaaaa gagcttgatt
     1861 ccctgcctga acatcatttg ttttcactgt cagtcgtaat gtcacttcat ttggtttggc
     1921 atatggtgcg atcgttggat tgatctgtgt ctcaatcaga tcttttagct cagtgaccag
     1981 tcgcgattca ccaatcccat aaaagcgcaa aactttagaa attagttttt cttcagaagg
     2041 aaaacgttcc tctagtaaag gacgtacttg ttccacaaac attggtttca actcattagg
     2101 cggaccggga agaaggatat acgcatgtgt atctgtttga taaaaagtcc ctaaagcaag
     2161 ccctgtacga tttggtagag gaaccccgcc ttcgataatc tgggactgtt gcagattgtt
     2221 tttggtcatt tttcgatggg ttgtttcaaa atatgccaat aattttttgt atccttccgt
     2281 attttgtatg agagacttac cgaggtgagc cgcagttgct tcttttgtca ggtcatcttc
     2341 tgtcggtcct aatccgccac ataagagtat gagttcactt cttgtttcag ctagagcaat
     2401 caactcttct aaacgctgct gattatctcc tacaactgtt tgataataaa cttcgatacc
     2461 tagatcagct agctgttctg acaagaaagt ggcattcgta ttcacgactt gtcctagtaa
     2521 aagttctgtt ccgacagcta ttatttctgc tttcattact tcccgctccc ttatatagta
     2581 tacgctgtat ttttatattt ttttatttaa aaagagggca aggagctgtg acaaaaatct
     2641 tgtcacagct ccttgttcca aataaacagt gttctcctgc caaaacctcg gtcataagtc
     2701 acaatataca taaaacatga aaagctgttt tttgaaatta tcccttgtga ctcagagtta
     2761 gacggcaggc tcacaacctc tccactaaac gatacttaca ttgatccttt gaacacaccg
     2821 ctgtttttga tgaagtaatc cacacctgaa tagatagtaa agatcagaca agcataaagc
     2881 atgatttgat ctactggaat accgatcagg ctgaatggga tattattgat aaacagcaag
     2941 ataatagcaa ccatttgtgt agccgtcttg acttttcccg gccaagctgc agccatgact
     3001 tctccatgtt cgacaagtag taaacgcaag cctgtgacgg ctaattcacg acagacaatg
     3061 atcgctacta cccaaccagg ggcctttcct tggtcaacca acatgataaa agcagtcata
     3121 actagcattt tatctgccaa cgggtccgca aacttgccaa aattagtgac aaggcctcgc
     3181 gcacgggcaa tttttccatc taaccagtct gtgatactag ctaaagcaaa aataactgca
     3241 cctactagct gtgtgacagc cagtgtggtg tctcctacgt taaattgtcc ccaatctaaa
     3301 ggtaaaccta caaccaaaat gaaaatcggg atcatacaaa ttctaagtac agttaattta
     3361 tttggtaaat tcacactatc gactcctttc ttccctattc tacgtgacta tcagcgaagt
     3421 ttccaccgat attatttact tttccaaatg aatttattca aaggagtatt tttattgaaa
     3481 ttgatctttt tgaattaacg aagaaggacc gcgacacaga tttgtcacga tccttctttt
     3541 ttatacctac aagtaaacag atgcgtaaac taatttatac gatcattctg cgtattgaag
     3601 agtaaggttc acattttttg gactctgatt gttttcccct ggattaactg gtacggattc
     3661 tccattcgct ttgatcgttg cattgcttgc tgcaccaatc gtgatggtcg catttgttgc
     3721 tgtttccggt aacacggtac tctgtgtttc atttgctgcc agtgtccctt gataaacata
     3781 ggcattgtct actagcacac ctaaccatac acgattgaca gcagtaaatt caagggttac
     3841 aggcttggta gcgtctgtga tatttactgc gatggcactg ccagaatctt ctcctgtagc
     3901 tattgccatt ttcttttctt cacttgattt tgttgttgat tctactgtag aagactcttt
     3961 ggtctgcgtg ctttgctctg aggaagagga ctcctcgact gatccttcta ctttaagcga
     4021 agaagctgtg tctccgatta ttggatcagc ttgacgatct tgccaagtca tatagcccac
     4081 gacaacgaca atcgccaaag caaccaatcc taataaaatg acaggaagac ttgtccaaaa
     4141 gcgacttgga tgcgattctt cctgatgcat tgcttttcta gaagtactta ctgtttcagg
     4201 ctgcggacgt tcagggaccg atcctgtcaa tttgcttttc ccatcaaaaa catcgactaa
     4261 atggtctcca tcttctccta cagcttcagc gtattgccga ataaatgccc gcacgtaaaa
     4321 tttaccaggc aattggtcaa aatcatcatt gtcgattgct tccaaataac gtttttgaat
     4381 ttttgtaatc tgctgtaatt catctaaaga tattttttta ttcaggcgtg cttgccgtaa
     4441 tttttctccg atggtttcaa aagccactcg ttcatcttct ccagtctgtt tgattctccg
     4501 aagcatctgc tgttgcaacg aaagacgtta tgtttgggtg ctgtgacaaa agtcatgtca
     4561 cagctccttt tttccgaata aacattgttc tcctgccaac tcctcggtca caagtcacaa
     4621 tatccaataa atataagaag ctgttttttg aacttgttcc ttgtgactcg gagttaaacg
     4681 gcaggctcac gacctctttc ttaaacggtg ttcaatcagc tgttcctcgg tattagctca
     4741 aaaaccggca aaatataagg agatattttt ccgtatgttt ctctaatact caggtcaaaa
     4801 cgctgatttc acaacctctt ccttaaacgg tgttcaaaag cttgcatctg cggtaataag
     4861 tccaaccaag ttaaaaacat taagaagctg tttttacttg gctgttctta ttacttgatg
     4921 cagggcagct ttttcacaac ctcttggtac acggtgttcc agaagtagct tcttcggcaa
     4981 taagccgaaa tatcacaaaa atttgagaag caattttcgt aaa
//
LOCUS       DAXWOO010000170         4959 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.174, whole genome shotgun sequence.
ACCESSION   DAXWOO010000170 DAXWOO010000000
VERSION     DAXWOO010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.174"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            96..809
                     /gene="treR"
                     /locus_tag="ACGE70_002486"
     CDS             96..809
                     /gene="treR"
                     /locus_tag="ACGE70_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFF8694089.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            886..1332
                     /gene="msrB"
                     /locus_tag="ACGE70_002487"
     CDS             886..1332
                     /gene="msrB"
                     /locus_tag="ACGE70_002487"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFF8694090.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            1502..2104
                     /gene="ruvA"
                     /locus_tag="ACGE70_002488"
     CDS             1502..2104
                     /gene="ruvA"
                     /locus_tag="ACGE70_002488"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFF8694091.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            2117..3118
                     /gene="ruvB"
                     /locus_tag="ACGE70_002489"
     CDS             2117..3118
                     /gene="ruvB"
                     /locus_tag="ACGE70_002489"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFF8694092.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            3147..3725
                     /locus_tag="ACGE70_002490"
     CDS             3147..3725
                     /locus_tag="ACGE70_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFF8694093.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKVLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            3777..4259
                     /locus_tag="ACGE70_002491"
     CDS             3777..4259
                     /locus_tag="ACGE70_002491"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFF8694094.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIAIEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            4376..4750
                     /locus_tag="ACGE70_002492"
     CDS             4376..4750
                     /locus_tag="ACGE70_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694095.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         1702 a          850 c         1082 g         1325 t
ORIGIN      
        1 ttaaaataag cttttcttaa aaataggaac attgaaatac aaaaagagct gtagtatagt
       61 gaagaaatga tggaagaacg agtgagggaa aaaacatgaa taagttcaat gaaatttttt
      121 tagatttgga acagaaaatt ttagcgaaag aatatcctcc gcattcgctt ttacccagtg
      181 agaaccaatt gatcaaaatt tatggtgttt cacgagaaac aattagaaaa gcgctgaatc
      241 ttttgactac ggaaggttat attcaaaaaa aacaaggaaa aggttcgatc gtactggatc
      301 ggaatcgctt cgactttcct atttctggat tgactagcta taaagagcta caggagaccc
      361 aacgtattcc aagtgaaacg atcatccata cgttagaaga aacagaagtg acaaaagcat
      421 tgaacgagat cactggctgg gagatcggtg cgcctgtttg gcatttgata cgcgaaagaa
      481 aaatcaatgg cgaagtagtt atattagaca ccgattatct attaaaggaa atcgttccta
      541 ttttaactcc agcacaagca caaggatcga tctatgaata ttttgaaaat gagctttctt
      601 tgacgattgc ttacgcgcaa aaggagatca cagtagaaga agtaacggaa atcatgaaaa
      661 cagcgatgga tcttcacgat gacacttatg cagtagtagt cagaagcctc gttcatctgg
      721 aagatacccg ctgtttcgag tattctgagt cggttcaccg attagataaa ttccgtttcg
      781 ttgagtttgc ccgacgccga aaagtgtaaa tagttcaaaa gaaagcaaat tgttggcatc
      841 tgtccacttt ggcgctatga taggatcaca aagggggcat ttttaatgga taaaccaata
      901 agaccagaaa aagaagaact gaaagaaaag ttatcaccca tggcttatgc agtcacacaa
      961 gaaaatgcaa cagaacgacc atttagcggg aaatatgatg atttttacga aaaggggatc
     1021 tatgtagata tcgtcagcgg tgaaccgtta ttcagctctg cagaaaaata tgatgcaggt
     1081 tgtggctggc catcattcag caaaccaata acgaaaagac aagtgcgcga aaaagccgat
     1141 ttttctcatg gcatgcatcg aatagaagtt cgaagcaaag aagcagattc tcatctcgga
     1201 catgtgttta ccgatggacc agtagatcaa ggtggtctac gttactgcat caattctgcc
     1261 tcactgaaat tcattccata tgaaaagatg gatgaattag gttatgggga attcaaatca
     1321 cttgtagaat aatggaaata cacaaagagc ggtacaatac ggggatttac tcgatcgtcc
     1381 gctctttttt tattcaagcg attgctattt gaaagaaacg ctccccagac tccttttttc
     1441 acaaggtgaa atgtgctaca ataaagggaa cgtatgtgtg caaaagaaag aggactagaa
     1501 tatgtatgaa tatctgacag gtgtggttac ctttatcaac ccctattaca tagtagtaga
     1561 aaccaatggt atcggctacc agatcgcact aggaaatcct taccgctaca gtagcaaact
     1621 aaatcaaaaa ataaaaattt atgtccatca ggtaatacgt gaagatgcgc atcttctgta
     1681 tggatttgat tcgctagaag aaaagcaact gtttttgcga ttagtaagtg tttcaggaat
     1741 cggtccgaaa agtgcccttg caatcatggc aagcgaagac cactccggtt tgattcaagc
     1801 agtagaatcc agtgatgtca cttatttgac gaaatttcca ggagtaggga aaaagacagc
     1861 acaacaaatg atcttggatc taaaaggaaa attcggtgag ctgagtatcg atacaccttt
     1921 tagtctattt gatgaagcaa acacaaaaga cgctacagct ctatctgaag caatggaagc
     1981 tctctcggct cttggttaca gtgatcgaga aatcaaacgt gtagaaaaac aattaaaaga
     2041 agtagataat cagacaacgg atgagtatct acgccaagca ctcaagctaa tgatgaaaaa
     2101 ataaggagga gaccaaatga cagatgaata cttgttatca ccagaatctg gcgaaaatga
     2161 actgagtttg gaaaaatctc ttcgaccaca gctgctttct caatatatcg gacagagaaa
     2221 agtcaaacaa gaacttcaga tttacatcga agctgccaga aatcgggaag aacctttaga
     2281 tcatgttctg ttgtacgggc ctcctggatt aggaaaaacg acgatggcca tggtcattgc
     2341 taatgagatg accgtaaata ttcgtacgac gagtggccct gctatcgaac gtccgggtga
     2401 cttagtagca atcttgaatg aactggaagc aggggatgtc ctgtttatcg atgagatcca
     2461 tcgattacct agagtggtag aagagatgct ttattcagct atggaagatt tctatgtaga
     2521 tatcatggta ggacaagggc caaccgcaca tccggttcat ttccctcttc ctccctttac
     2581 tttgattggc gcgacgacac gggcaggtat gctttctgca cctttacgag atcgatttgg
     2641 catcatttct catatggaat attatgacga acaggatctg caggagatcg ttttacgttc
     2701 ttcggatatt tttcaaacag agatcattga agaaggggca ttggaaattg ctcgccgatc
     2761 acgaggaacg cctagaatcg ctaatcgctt gctcaaacgg gttcgtgatt ttgcgcaagt
     2821 ccaaggagac gggaaaatcg atcgtcctat agcggatcag gcacttactt tgctacaggt
     2881 cgatcaggct ggattggatt atgttgacca gaaattgttg cgaacaatga tcgagttata
     2941 tggcggcgga ccagtagggt taagtacgct ttcagtaaac ataggagaag agcgtgagac
     3001 ggtagaagat atgtatgaac cgtatctgat ccaaaaagga tttttaaagc gtacacctag
     3061 agggcgaatt gctactgctt atgcctatga acattttggt tacgactatc atatatgatt
     3121 tttgctttta gattggagga aaatgaatga gtgatacaac acgtacgaaa caattgatca
     3181 aaaacgtatt gattgatttg tgccaaacgc aatcttttcg aaaaatcagc gtccaaaaaa
     3241 tcgcaaatga agcaaaaatc aatcgacaaa cattttatta tcattttaca gataaatacg
     3301 atttgcttcg ttgggtctat tgtgaggatg cattgcatta tttagatcca caatgtcttt
     3361 cacttgataa ttgggaagag caagcattga aagtgctgaa agcaattgct gagaagaaag
     3421 aattttatgc aaatactgtg ctttctgacc cagaaatttt acaaaaagag ttttctcaac
     3481 tgatccaaaa atcatttatc aagatttttg aacaaatgga tgaagaaaga caactttctg
     3541 caagagacaa actattttat gctcgttttt tttcttacgg ttgcagtggt gtattagtca
     3601 attggatcac gaatgaatat gaagaaacac cgatggaaat cgctatgcag cttttccgtt
     3661 tagccaagga tacggaatta tattcttatc gtctttatgc aaaagaagag ttggaagaac
     3721 agtaatagaa aaattttttg ttggaaaagc aataatcgct aaaaggagat ggggaaatga
     3781 caaaaatttt atttgtttgt ttgggaaata tttgccgttc accgatggca gaaggcttgc
     3841 tgagaaaacg aatagcaata gaaggaaggg aagcagaatt ttttgtggat tctgctgcta
     3901 caagcacgta tgaagttgga aagacaccgc accctggaac gaaaaaaata ttaaaccaag
     3961 aaaaagtaga tatgacgaat atggttgctc ggcaaatcac accccacgat tttgaaactt
     4021 ttgattggat cattggcatg gatcaagaaa atgtagaaga actgaaacga agagcaccac
     4081 gctcagcgca ggggaaaatc catttgtttt taagtagcat tcctggaaaa gaaaaagaaa
     4141 atgttcccga cccatactat accaataact ttgatgagac atatcacttg atcacagaag
     4201 gcctgaatca ttggtggaat atttggattg acgaaaataa aataacaaac aatttgtaaa
     4261 gttgacgctt tctttttaag gcgatgttag agcatttcct tgtttttttg taaaatgtta
     4321 ggtaaaataa aattaattaa gtaacacttt cttagctaaa ggaggagtgc attcgatgaa
     4381 cgaagagaca gtatcgccaa tgaagaagct tacgaagtta gttactcata cttcgtatca
     4441 actttttgat tcttttgtaa atgacactga agaacatgct aagaatgtac gattgcaagg
     4501 aaagtttatt cttgagctgc aaaaagctgc tactgaacat agtctagtta tcttacaagt
     4561 aagagcgaat cgtttgagta gcaagtatga aacgatctca ggctgggtgg cgacaaaaac
     4621 ggtcgaaaaa gaccgagtga tgatccgcct acaaaacgaa gagcagcaga tccggatggt
     4681 tcctgtagaa caaattgaaa aaataacaat cctttcacca gcaggggaac aggaacgatt
     4741 atcaaaataa tttataccag atagaaaagc acgtatgagc ttgtaccatc aatcattact
     4801 aatcaaaaat aaccgaatgc tttaagctga tatcagtaaa acggaacgtt ttaggatgag
     4861 tatcacagca tgacagcatt gggttgatta gttgaatcga ttggatggta caagtttttt
     4921 tgataaaaaa aggtcctgct agccggggag ctagcagga
//
LOCUS       DAXWOO010000171         4924 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.175, whole genome shotgun sequence.
ACCESSION   DAXWOO010000171 DAXWOO010000000
VERSION     DAXWOO010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.175"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(55..879)
                     /locus_tag="ACGE70_002493"
     CDS             complement(55..879)
                     /locus_tag="ACGE70_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFF8694096.1"
                     /translation="MNKRKWLFVCVAALFSTVAYSQLSFADSSDSSTTQTIDNSITAP
                     SETDSSENTTEETADSNISFTESTNPTSESSSETSEKPQDVEDTQVIKQAIHIQKIIS
                     SNQINNQGSQVAQQEGINGAKFIVYDITNILEEMSQDDNEKNQSVEIIESKLKDRAKK
                     LSYDQLKKVAEGETKTIDDQEGVIEFSIEVPANKKQAYYIVNESSPENISNSEDIVLL
                     TPVSNEKGEFLTDVWLYPKSEKSEPKEEIKKVVSTGVKKNFFENCWEFFAQLFFDN"
     gene            complement(876..1631)
                     /locus_tag="ACGE70_002494"
     CDS             complement(876..1631)
                     /locus_tag="ACGE70_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFF8694097.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPVVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFCVPIIFIYKKIRRRSE"
     gene            complement(1644..3620)
                     /locus_tag="ACGE70_002495"
     CDS             complement(1644..3620)
                     /locus_tag="ACGE70_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFF8694098.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGGLSAVVYFKKKA"
     gene            complement(3673..4344)
                     /locus_tag="ACGE70_002496"
     CDS             complement(3673..4344)
                     /locus_tag="ACGE70_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFF8694099.1"
                     /translation="MKKWLFGFLGVTLIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPGYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            complement(4404..4613)
                     /locus_tag="ACGE70_002497"
     CDS             complement(4404..4613)
                     /locus_tag="ACGE70_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694100.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYRIHF"
     gene            complement(4680..4919)
                     /locus_tag="ACGE70_002498"
     CDS             complement(4680..4919)
                     /locus_tag="ACGE70_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694101.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKALSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
BASE COUNT         1326 a         1032 c          737 g         1829 t
ORIGIN      
        1 ggctaaaaag gatagattaa aaactaatat tcttattatt ttcatcattt catcctaatt
       61 atcaaaaaat aattgagcaa aaaattccca acaattttca aaaaagttct tctttacacc
      121 agttgacaca acttttttaa tttcttcttt cggctctgat ttctctgatt tagggtataa
      181 ccaaacatcc gtcaaaaatt cacctttttc atttgaaaca ggtgtcaata aaacaatatc
      241 ttcactgttt gaaatgtttt cagggcttga ttcattaacg atatagtatg cttgtttttt
      301 gtttgcaggt acttcaatgg aaaattcaat cacgccttcc tgatcatcaa tcgttttggt
      361 ttctccttca gccacttttt tcaactggtc gtatgaaagt ttttttgctc gatctttcaa
      421 ttttgactca attatttcta cagattgatt tttctcatta tcatcttgtg acatttcttc
      481 taagatattc gtgatatcgt agacaataaa cttcgcgcca ttgatacctt cttgctgggc
      541 tacttgactg ccttgattgt tgatttgatt cgaagaaata attttttgaa tatgaatggc
      601 ttgcttgatc acttgcgtat cttctacgtc ttgcggcttt tctgaagtct ctgaagaact
      661 ttctgacgtt ggattcgttg attcagtaaa gcttatattt gaatctgccg tttcttctgt
      721 agtattctcc gaactgtccg tttcgcttgg tgcagtaatt gaattatcta ttgtttgagt
      781 ggtactacta tcactgctgt cagcgaaaga cagttgcgaa taagctacag tagaaaagag
      841 agctgctaca caaacaaaca gccattttct cttattcatt ccgatctcct ccttattttt
      901 ttataaataa aaataatagg aacgcaaaaa aaagcgaata gcagtgctaa tcgccaatcc
      961 aatgaaattg atttcctatt ttttgctttt ttctgtagtt ttaaatcttc tttactgaag
     1021 gaaactcgtt caccatacac taaaaagcgg tgtgagttga caccttttgg tgtgcatgtc
     1081 attaacgtga tcctatcttg gtctttttca ggcataagat aatctaaata atttttttgt
     1141 aattcttctg ctttaacttc ttcaattcga taaatttgat aggcatgttg ttcttcattt
     1201 accttaataa agaatttttg cccttttttt actgaaggca aatcatcgaa aagattatct
     1261 ttataaagtc cactgtgccc cgcgattaaa ggatttttac cgtttcctcc tgtaatatcg
     1321 ccagttcctt ccgtaatccc tacaccattc tctaaaattt tgtcggaagt tccgtcataa
     1381 attggtagtt ttaaagaaat acttggaatt gtaagaacac caacaggatc gcctaactct
     1441 attttcgcaa catccaaaga agaatctgtt ttttcaagat tttcaatggc taaaattctt
     1501 tgctgttgtt gaaatttcgc tacatttgtt tccacaactg gaattaaata gactgctact
     1561 gccgacagca accaaaaaaa tatcatcagt ttgtgcatga ctcttctttt tgggggctta
     1621 cgaataatca ttagctgatc cttctaggct ttcttcttaa agtaaacaac tgcagaaagt
     1681 ccccctgctc ctagcaacaa gcaagagatc aatacgtaag attggaaacc gttgctacca
     1741 gtcattggta cggaagcttt ttgattattt ttgatcgtga ttcggttttc ttctttgtat
     1801 gaatctttgt ctactttgaa agagatctct tttgttaatt tttgatagtt ttcaggagct
     1861 tttgtttctt ccagtgagta atctccttca gttaaaccag tgatttcaaa atgaccctct
     1921 ttatctgatt ttaggattgc tgcatttgct acatcgtcta cccaaactgc ttctttgaca
     1981 ccatctttat ctgtatcagt aagtccaccg tagtacttac cttctgcatt cttagctgca
     2041 aattctgcgc cttcaagcgg tgttgctttg atacctgttc caaataggcc gctatcgact
     2101 tttaagaatt ttgcgccata agtcacaacg tcagctttcg ttttttcgtg gtgttcttcc
     2161 tgattaaatg gattattgtt ataaacgaaa tccacgctat ttgttaatgg atttgcgatt
     2221 tcagcactat cattcacaaa catgttataa gtcattttta gatcttgacc atgtaaattc
     2281 gcaactgtat cacttacttg tccattattt acgataaaat cgatcacaaa accactatca
     2341 gaattttcga ttttatagtc agtattttct tttaatacga ctgaaccatc attatttgtt
     2401 actttaatat ctgctttttc agcaacataa gaaagtcctt tacctgctgt atctccccat
     2461 ttaaatacac tgtaaggaga agtttcgctt ttcacaccgt ttactgtgat accgtctgcg
     2521 ccacctgcaa tgtctgttgg aatatgaatc gttccttgat aatttactgc gtcaccaatt
     2581 tggaaagaat gttcttgtcc tgttccattt gttactgttt tatcgatcac tggtgcttta
     2641 tagtttacgt atgaagcttt taagtcactt gtcacgccat cattcgtaat ttcaaaggtt
     2701 aatttattgt gcgcatcgtt gcgagcgtct gcgcctaata aatattgatc tgctgttggt
     2761 tcttgatctg atcccactac aacttccgaa ggaacttcca caaaatagta ttttcctaac
     2821 gtcaaatcag tcgcagtcag ctttccttga tcatccgata caagatcacc caattttgtt
     2881 ccttttccag gttcgccctt gtataactca aattttgccc cttttaaaag ggtttgtttg
     2941 ctttggtctg cgccatcgtc accaaatttc gttaattcaa aactcaactt agaaacaatg
     3001 ttcttaggat acaaatgaat attttttaaa aatcctgatc ctgttttatc tgtcattggc
     3061 gcaatcacaa ccattggttt tgctttttgt gtgactaatc cagcagcgga tttactttca
     3121 taaaccacat atacattccc tttggaattg acataggctg gttgatcagt aaaagtgatc
     3181 tcaccatttt catctacagc tgaagtaatt ttgttggttg cattcttgac gtactcactg
     3241 ttagatccct tgtcttctac atcttgtaca atctcgtcca ctttcgcgtt ggttagatca
     3301 gaatcttctc tcggtaatac ttgatccgta atatttccta acgtaaactc aacatcacca
     3361 taatctgcct tgttgtattt cgttacacct gtaggatatt gatcgtgcgt ataaccatcg
     3421 tttttgatcc cgtcaatatc aacattcaat tgcgttcctt gatcgtacat taatttatga
     3481 accgtgatat tgaccttctc tggtggtgtt ttttgcgtga catcttctgc atgtacttga
     3541 tacccctgaa agcagcctgc aatgattcct aacattccaa caagcattcc agcttttttt
     3601 acataccgat ttattgccat tttttactct cctcatttca tatttcaacg actcgcgttg
     3661 attaaaacct tgttattgta gctctccctg taccacgtat ctttcttttc cttgtgacgt
     3721 ataaacacaa gtaaacaacg ttaataaggg tttctctcct ttctttggca aagacaagaa
     3781 agagttctct tcaggaacgc catcgattaa tttaaagtac ccaggtacga taaatttacg
     3841 cgttaccttg taacgataga cgccatcgtt actaactaag tccatcacct cccctaatga
     3901 caacttttga atatcagaaa ataaaacacc tggcatttcc atgttatgac ctgctaggac
     3961 atagtttcct ttgcccattt cggcatcttc ataaaaataa gtagccgctc caagactcaa
     4021 agtgtattga tttgcccctt taaaaattgg caatgctaaa ccaagtgcag gaatctgaat
     4081 ttgtgcaaat acttgtttat tgacgcccat ttcgattgct tctttttgta tggacaacat
     4141 ttgccccaaa gacatatcat tattgacgtt cgttgcatcc tccttataac tctttgcatg
     4201 tgcattgatt tgatctacgg gcatcttttt ttctatattt tgcttaaaca cctctccttc
     4261 atgtttttgg tagctaacat agccaaacac actgcaaaag acgattaaag tgactcctaa
     4321 aaaaccaaat aaccattttt tcatccatct gcctcctttc agacaaaaca aaaaaacttt
     4381 ttctcatttc catagaaaaa gttttaaaaa tgaattctat aagttcgtct caactgttca
     4441 aagtttttca ttgaacctaa caaacaagct ttctcttttc ctgttaactg catatcattg
     4501 atatattgcg cgacagataa tcttttaagg tgtttttgct tcatcgtttt tagtaacttt
     4561 tcttccattt ctttcgacac tttttttctg ttttcgattt cttcataatt catgagtttt
     4621 cctaccttcc ttgttttttg caaaaataaa aaagcctatg atcacatacg ctctacactt
     4681 cagtcttggg ttatcttttt ctttcgttta attactggcc gtgctggttt caaatccttg
     4741 attccattat gggttctcca ataatcgaat tcttttcgca atctttcttt ttccatatct
     4801 gttcctttag ataaggcttt gaatacttct ttaggtgaat aaccacgagc taacagctgt
     4861 acataccttc cttttgtcaa agtatccata tccatcgcct ctaaaactgg tcgcgccatc
     4921 tact
//
LOCUS       DAXWOO010000172         4917 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.176, whole genome shotgun sequence.
ACCESSION   DAXWOO010000172 DAXWOO010000000
VERSION     DAXWOO010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.176"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            19..1308
                     /locus_tag="ACGE70_002499"
     CDS             19..1308
                     /locus_tag="ACGE70_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFF8694102.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            1321..4023
                     /locus_tag="ACGE70_002500"
     CDS             1321..4023
                     /locus_tag="ACGE70_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFF8694103.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            4008..4865
                     /locus_tag="ACGE70_002501"
     CDS             4008..4865
                     /locus_tag="ACGE70_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFF8694104.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
BASE COUNT         1737 a          850 c         1069 g         1261 t
ORIGIN      
        1 agaaagaagg ttttattagt ggcttataaa gatataccaa aatcggtgca gaaatttatg
       61 gaagatatca cacaaaaatg tggggatatc catgcggact gggcaaagaa ttttacagca
      121 gcatttgcca atactctgtt gacaacagtc aaaaagcaaa atgacggaac caccttttta
      181 ttgacgggag acattccagc gatgtggtta agagactcta ctgcacaagt tcgtccttat
      241 ttagtgattg caaaagaaga tgaggaatta gctgacatga tcagcgggtt agtcaaaaag
      301 caattttttt atatcaatat cgatccatat gcgaatgcct tcaacgaaga agctaacggt
      361 gctggccacc aaacagatca tacaaaaatg aacgattgga tctgggaaag aaaatatgaa
      421 attgattctt tatgctaccc aatacagttg gcctatcttc tttacaaaaa tacaggaaaa
      481 actgatcaat tcaatcaatc ttttattgaa ggtatcaaaa aaatcttgga cgtgttcaaa
      541 acagagcaag atcatgaaca ctctccttat acatttgtac gagacacgac aagaaaagaa
      601 gatacgctga tcaatgacgg gcgaggtgca aaaacagctt ttacaggaat gacatggtct
      661 ggtttccgtc ctagcgatga tacttgccgt tatggatatc tggtaccatc caatatgttt
      721 gcggtggtcg tgttaggtta tgtggaagaa atttttcaaa cgatactcga tgataaagaa
      781 attgtacagc aaacacagaa attgaagaaa gagatccaag aggggattca gaatcacgga
      841 cgaacggtta atcaaaaagg gcaaacgatt tatgcatatg aagtagatgg aatgggaaac
      901 gccacagtca tggatgacag taatgtgccc aatctgatcg ccgcacctta cttgggatat
      961 tgttctgaac aagaggaaga ttatctgcaa acaagaagaa cattgttgag taaagaaaat
     1021 ccatatttct atgaaggaaa gtacgcaaaa ggaattggat cctcccatac gccagaaaaa
     1081 tacgtttggc cgattgcatt agctatggaa ggaatgacaa cgtcagacaa atctgaaaaa
     1141 gagcggatat tgaatttact tgtagcaact gacgcaggaa cacaccttat gcacgaagga
     1201 ttcgatgtga acaatcctga aaattatacg agagaatggt tttcttgggc aaatatgatg
     1261 ttttgcgaat tagtcatgga ttactttgat attagagtag aaaagtagga ggtatcgtaa
     1321 atgaaaaaga aagtatatat catttctcat agccactggg atcgtgaatg gtacatggct
     1381 tacgaacagc atcacatgcg actgattgaa ttgatagacg atctactaga gctatttgaa
     1441 gttgatccgt ccttcaacag ttttcatttg gatgggcaaa cgattatttt agatgactat
     1501 cttcaagtaa ggccagaaaa aagacaggca atccaacaag caatcaatga aggaaaattg
     1561 agaattggac cattttatat cttgcaagat gattttctta tcagctcgga atcgaacgtg
     1621 agaaatatgc tgattggcat ggaagaaagc cgtaaatggg gaacccctgt catgttaggc
     1681 tattttcccg atacatttgg aaatatggga cagacgcctc aattaatgaa acaagctggt
     1741 atttcagcag cagcgtttgg ccgtggtgta aaaccaattg gctttgataa tcaagttttg
     1801 gaagcagaaa actattcttc ccaatactca gagatgtggt ggaaaggacc tgatcaaaca
     1861 gcaatctttg gtctgctatt tgccaattgg tatagcaacg ggaatgagat tccagtagaa
     1921 aaagaagcag ctcttgcctt ttggaagcaa aaattagcgg atgcagaaca atatgcctct
     1981 acgaaccact tattgatgat gaatggtgtt gatcatcaac cggtacaaaa agatatatca
     2041 aaagcaatcc atctcgcaaa tgaactgttt ccagattacg agtttattca ttctaatttt
     2101 actgactatc tggaagcagt tcaaaaagat gtgccagaag acttaggctc agtagaagga
     2161 gaattgacaa gtcaagaaac ggatggctgg tatactttag ccaatacagc atctgctcgg
     2221 gtttacttaa aacaatggaa taccaaggtc cagcgacagc tagaaaatat tacagaacca
     2281 cttgcgacga tggcttatga agttagtgga aactatcccc atgatcaatt agattatgct
     2341 tggaagacat taatgcaaaa tcatcctcac gacagtattt gcggttgttc agtcgattct
     2401 gtccatcggg aaatgatacc tcgttttgaa aaagcagatg aagttggaaa gtatcttgca
     2461 caagattcct tagaacagct gaccgcagca atcgatacga ccggattccc aaaagacagc
     2521 tttccatttg tcattgtaaa tactgcagga atggataaaa ctggtgaagc agaaataacc
     2581 atcgaattag aaagaaaaag attcgctgaa ggaatacctg aacaactata tcaagagtta
     2641 gaaaatttgc ctaaacgtaa gtatcatgta gaaacaaaaa gtggggccac tataccagct
     2701 attttatctg aagaaactgt ccaatttggt tatgatttgc cgaaagaccg tttccgtgtg
     2761 ccatatatgg caagaatgat caaagtgact ttaccattag aaaacatgcc agcattttct
     2821 tgggaaacat tcgcgcttgt agaaggggaa gcgaaagcag aagaaaagga aacaatgatt
     2881 catcagagtg gacgaatcat cgaaaacggc cctctacatt tgacgattga aaaaaatggt
     2941 accattacaa tggaagatcg caaaaacaaa cgaaagttga ataatctgca tatttttgaa
     3001 gatatcggcg atataggaaa tgagtatatc tttaaacaac ctttctgtga tcagccaatc
     3061 ctatccagta acaaagaaaa ctctgaagta aaagtattag tagatacacc ggaaatagca
     3121 aaaataagta ttctacagga aatggaaatc cctgtttctg cagacgagcg tttagaaaaa
     3181 gaacagcaga tggttgtgga atttcgttat cgaaaagcag aacgatccaa agaaaaacga
     3241 atccttcaaa taaaaacaat catgacaatc agaaaagata gtaaaaaaat cgactttgaa
     3301 acgactctcg acaatcagat gaaagatcat cgcttacgtg tcttgtttcc tacaaagtta
     3361 catgtggaac atcacgaagc agatagtatt tttgaagtgg tgaagcgccc aaaccatgtg
     3421 tctaaaagtt gggaaaaccc cacgaatccg caacatcagc aagcatttgt caatattcat
     3481 gatgaagagt acggtgtaac agtcggaaac tttggtttga atgaatatga agtaacagaa
     3541 gacggacaaa tcgcagtgac gctacttcgc agtgtgggag aactaggcga ttggggatat
     3601 tttccaacac cagaagcaca gtgtctaggt gagcatcggt tcaattacag cattgaacta
     3661 catggaccag aagaaaaatt ctctacttat ttgcatgcat acgcagcaca gattcctttt
     3721 tccactcaac aaatcaaaca tcatgaagga acattgattt caaaacaaca atatttaacg
     3781 ataaaaagtg aaacgtttgc aattacagct ttgaaacgca gcaagtttag tgataaagta
     3841 gtagtaagag gtttcaatat gtctagtcat ttagaaaaat tggaaataac taaagacaat
     3901 ggaaaaactg tcatattgaa tctgcttgaa gaaccaacga agcaagcggt tgtcccaata
     3961 atccaacctt atgagattcg gacaatcgga ttcgaggagg aaaacgaatg tatggcggaa
     4021 tagaagcagg cggaacaaaa tttgtgtgtg cggtttcaga tggtggagaa attttagaaa
     4081 aattaagtat ccctaccaca acaccagaag aaacattgga tcaggtattt gaattttttg
     4141 atcgttatga attgaaggga atcgggattg gctcattcgg cccgattggt atcgacaaaa
     4201 caaaagataa ttacggatac gttttggcaa ctcccaaaaa agggtgggca caattcgatt
     4261 ttcttggcac aatcaaggaa cgctacaata ttcctgctgc ttggacaacg gatgtcaatg
     4321 ctgctgcata tggggaattg aagcaaggag cagcacaagg aaaaaatagt tgtatttacc
     4381 ttaccgtggg aacaggaatc ggtgcaggtg tcgtgatcaa tgaagaaatt ttttcaggga
     4441 tcgcacatcc tgaaatggga catatttggg tgaaacgcca tccagatgac cattatgaag
     4501 gtacttgtcc ttatcataaa gattgtttag aaggattagc agcaggaccg tctattgagg
     4561 cacgcacagg gatcaaaggg caagatctgc cgcaagatca tcctgtttgg gctattcagg
     4621 cttattatat tgctcaagca ctgatcaact atacgttgac gttagcacct gaaaagatta
     4681 ttttaggcgg tggggtaatg aatcaggatc atctccttca aaaaatccgt cagcaatttg
     4741 ttgaattgat gggtggttat atggaaacac ctcaagtaag tgaatatatc gttcgttggg
     4801 gactgccgaa tgaaagtggg attatcggta gtttgctgtt agcagaacac gaacatcaaa
     4861 agtaagcaga caaattacac aaaaagaaca gagtcttatc attatctgct gataaga
//
LOCUS       DAXWOO010000173         4793 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.177, whole genome shotgun sequence.
ACCESSION   DAXWOO010000173 DAXWOO010000000
VERSION     DAXWOO010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.177"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            59..174
                     /gene="rrf"
                     /locus_tag="ACGE70_002502"
     rRNA            59..174
                     /gene="rrf"
                     /locus_tag="ACGE70_002502"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(227..697)
                     /locus_tag="ACGE70_002503"
     CDS             complement(227..697)
                     /locus_tag="ACGE70_002503"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFF8694105.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(856..1314)
                     /locus_tag="ACGE70_002504"
     CDS             complement(856..1314)
                     /locus_tag="ACGE70_002504"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFF8694106.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1485..2630)
                     /locus_tag="ACGE70_002505"
     CDS             complement(1485..2630)
                     /locus_tag="ACGE70_002505"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFF8694107.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2643..3560)
                     /locus_tag="ACGE70_002506"
     CDS             complement(2643..3560)
                     /locus_tag="ACGE70_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFF8694108.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3988..4551
                     /gene="efp"
                     /locus_tag="ACGE70_002507"
     CDS             3988..4551
                     /gene="efp"
                     /locus_tag="ACGE70_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFF8694109.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT         1500 a          881 c          917 g         1495 t
ORIGIN      
        1 tgacttcaaa tccagttttg agtgaacaaa atttactcaa taaaagataa caccacagtg
       61 tggtggcgat agcgagaagg atacacctgt aaccatgccg aacacagaag ttaagcttct
      121 tagcgccgat tgtagtgagg ggttgcccct tgtgagagta ggacgtcgcc acgcaaaggc
      181 aaaaagatga actagaaata gttcatcttt ttttattttt attaagttaa agaacggatt
      241 caattgcagc ggtcattttc aatggagaag tttgtggtgc aaatcgttcg attactttgc
      301 catttctatc aatcaaaaat ttagtaaaat tccattttat tgctttagtg acagtaccag
      361 gagcttcatt tttaagataa gtaaaaagag gatctgtatt ttctccattt acgtcaatca
      421 attgatgcat agggaaggta acgccataag tggttttaca agcttctgct gcttcagtcg
      481 ctgaatctag ctcttgttta aattgattgg aaggaaagcc taagataacc agaccttgtg
      541 tctgataagc ttggtaaagt tcttctaatt gtttaaattg aggtgctaga ccgcattttg
      601 ttgcagtgtt aacaatgatt atcggtttgc ctgcatattt tttcaatgaa tagacagtgc
      661 cgtcttctaa agtagtttca aaattataaa tagacataaa gttttctcct ttctatttta
      721 tataataaaa agagcgctaa actgaaaact agctatttgt tttcccgttt agaactcttt
      781 tcagacaata aatatttaat aaaatacgaa gataagatag ctatattgct tctcatcttt
      841 acgttctttt agaaactatg cttctacttc agaccattca tcgcgttttg ctaaaaattc
      901 acgagccaaa gcttgtgcac cttctagtgt atggctggca gcccagccac attgtgtttc
      961 attgcttgca ggtacttcag tagcttcaag aacatctttc attgttgctt caagaacatc
     1021 taaaatgtct tcgtagcttt catgattcaa tacagtcaag taaaagcctg tttggcatcc
     1081 cattggagac cagtctacga tgtaatctgc gtgattgcgg attagttcgg cagtcaagtg
     1141 ctcaagtgaa tgtagacttg gcatgtccat gtgttcttta tttggttgtt taaagcggac
     1201 gtcgtattta atgatgacat cgccatgcgc acccgttttt cgatcagcta cacgtacata
     1261 aggtgctttg acttttgtat ggtcaagatt aaaactttcg acattcattc tcatttgtaa
     1321 gactcctatc tgtttgttca ttcgttttga tgcttttcac taaagaatta tttagtgaaa
     1381 tttacaaaaa cgtctttcta cttgattcta acacttatcg tttccaaggt gtagcaatct
     1441 gaaatatttt ttagattttt ctgctaagtc gtttattggg acttctagtt cttgattctg
     1501 tcaaatgcct gagctaaatc gtttatgaga tcctgaccat actcaattcc tacagaaaga
     1561 cggatcaagc cgtcctttat gccagcctct tctcgttttt cttttggaat agacgcatga
     1621 gtcatgacag aaggaatttc cactaaactt tctacgccac ctaaactttc tgctaaagta
     1681 aatagttgga gtgattcaac aaatggaata gcagattctt cattttttaa agtaaaagaa
     1741 atcattccgc taaagccgct catctgctgc ttagcaactc catggagttc atgatcgggt
     1801 aatcctggat agtagacctt ttctactgct ggatgagaaa atagaaaatc agctaccaca
     1861 aaagcatttt tttgatgttc ctccatacga acacttagtg tcttgattcc gcgctgaagt
     1921 aaccagctat cttggggacc aagtacggct ccgatagcat tttggtaaaa tccgatctga
     1981 tcggctagat ctttatggtt agaagtcact aaaccagcta ctacatcgct gtgtccgcct
     2041 agatatttgg taccgctatg gacaacgata tcagaaccga gatctaaagg tctttgaaaa
     2101 taaggagtag caaatgtatt gtcagcaata actatcaatc catgttgatg agccagagtg
     2161 gcactttttt ctaaatcagt aatctttaga agcggattgc taggtgtttc aagataaagt
     2221 gcttttgtat tgggtttgat ggattgttct attttatcaa gattactcgt atctatgatc
     2281 gtgtactcga gaccattttt agtaagaact ttgtcaaata gacggaatgt tcctccatag
     2341 acatcatctc ctaataagat atgatcgcca gcttgaaaaa gagaaaaaac ggcatgaatc
     2401 ccagaaagac cagaagaaaa agcgaatcct cgaacaccgc cttccaaatc agcaatcagt
     2461 tcttccaatg caaaacgagt aggattacca gatcttgaat attcatattg tttaggctgg
     2521 ccgacgccat cttgccgata agtggatgtt tgataaatag gtacactgac agctcctgtt
     2581 gtgggatctt cactgatgcc tccgtggatc aattttgttt gaatgtgcat gtttctttcc
     2641 tcctataagt aaatgttttt tgataaatag cggtctgctg cgtctggaaa aatcgtaacg
     2701 acacgatgac caggtggtaa acgacgaatt tctcttaatg ctgctgcaaa agccgcacca
     2761 ctcgaacttc caactaatag tccttgttct cgtgctaact gcctagtata gttaaaccct
     2821 tctacatcag agattgtctc gatctgattg attgaaaggg gagaaagaaa cggcggaatg
     2881 aattctacac cgataccctc aatttcgtga ggggctggat caccaccatt taacacagag
     2941 ccctctggtt ctacaccgat tattcgaatg tcgggatatt tttctttcaa aaaggtagaa
     3001 gttccagcaa aggttccacc actcccaatt cctgctacaa aactatggat attttctttg
     3061 atttcatgaa agatttctgg ccctaacgta tgatagtaag cagcaggatt atctttattt
     3121 tcaaattgaa gaggcagata gctgtttgat atctcctctg caagtttttt acttttgctg
     3181 atggcaccga tgattccttg ctcactagga gaatggatga tctttgctcc caatgctttc
     3241 atcaattgtt gtttttctaa gctaaacttc tctggaacaa caaaaaccgt tcttaatttg
     3301 tatgtgagtg cggccagagc gatcccaatt cctgtatttc cggcagttgg ttcgatgatc
     3361 gtcgtttttt cattgatttt tccttgggca aatccttctt tgatcaaata actgcccaat
     3421 ctatctttga tacttcctcc aggatttaaa tattctagtt ttgcataaat agcagaacct
     3481 ttaggcatag aatagtgctg attgtcaaag cgataaagcg gtgtggaacc aatagcttct
     3541 gtaattgtcg agataatcat gtgggcctcc taaaaaaaga taataaaaaa aggagctgac
     3601 tattaaatca gctccttgaa agacatagag aaaaattgag tagataaaaa gataagttac
     3661 ctggtactca ttctatgaag ggcaattaat agacatgaca aatagtatgc tgcttttatg
     3721 caccatacaa cagcaacgat tcaattgttt ttggttgctg actgtcatgt gtatccctcc
     3781 aagtaaaatt tttattaacg atagcatgaa aaaaaataaa agtcaaaggc ttgaaagaaa
     3841 aaaatatttc gatagcatcg tttacccgtg tttatttttc tgacaaatag gcatgagacc
     3901 ttaaaagata gagaatactc tagttttctg gccggttgtc tgatacaata aagttgatta
     3961 tgataaagaa aagaaggagc aaaaataatg atctcagcaa gtgatttaag agccggtatg
     4021 actttcgtac aagacggcaa actgattaaa gttctggatg caagccacca taaacctggt
     4081 aaaggaaata cagttatgcg tatgaaactg aaagatgtcc gttcaggtgc aacatacgac
     4141 acaacgttca gaccagaaga aaaatttgaa aaagcacata tcgacacaaa acctgtacaa
     4201 tatttgtaca cgatggatga tacagctttc ttcatggatt tggaaacata tgagcaatat
     4261 gaaattcctg tagaaacagt agaagaagaa atgaaatata tcctagaaaa catggaagta
     4321 aaaatccaat ttttcggtag cgaagtcatc ggcgttcaat taccaacaac tgtcgtatta
     4381 cgtgttgtgg aaacacaacc ttccatcaaa ggtgcaacag tcactggttc aggtaagcca
     4441 gcgacaatgg aaactggctt agtagtcaat gttcctgact tcatcgaaac agatgaattg
     4501 ttggaaatca atacagcaga aggttcttat gtaaaacgtg cgtcaaaata attcgctaac
     4561 ggattgataa gaatttgaaa acagagcttg acctcttgaa aggcagatca tcggatctgc
     4621 ctttcaagag gttttttgtg gaatcagcaa attgaaaaat aaaaaagtat ttgattttat
     4681 ttatattttg aaagatgaat ggcaaaagct agagaaagaa aaaactgcac ttatttatga
     4741 agaaattgaa atgattcata aaattgaaaa tatccgaact attccgaact ata
//
LOCUS       DAXWOO010000174         4786 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.178, whole genome shotgun sequence.
ACCESSION   DAXWOO010000174 DAXWOO010000000
VERSION     DAXWOO010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4786
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.178"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..813
                     /locus_tag="ACGE70_002508"
     CDS             <1..813
                     /locus_tag="ACGE70_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="HFF8694110.1"
                     /translation="KKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSD
                     NGQIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAIKRVKSVSSG
                     DNFIMINWRIAETQTSFPWCAAYVEAAILCNKTDVHATSARAIMHYTYPNLNNSQIES
                     KSLNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHAL
                     ALFGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            786..1229
                     /locus_tag="ACGE70_002509"
     CDS             786..1229
                     /locus_tag="ACGE70_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694111.1"
                     /translation="MGQYHYKLVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLG
                     TWQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNL
                     LTLRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            2401..3369
                     /locus_tag="ACGE70_002510"
     CDS             2401..3369
                     /locus_tag="ACGE70_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFF8694112.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEEQNRALELANRQTQHEKETTSLQERIKEQKRKLELL
                     QQLEELEKQQKNRENETDKQKLDNNSDIPLELRKDLLDNLQNLFKD"
     gene            3379..3849
                     /locus_tag="ACGE70_002511"
     CDS             3379..3849
                     /locus_tag="ACGE70_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFF8694113.1"
                     /translation="MTDKSIKEFAEELGVSKQAIRKYFDKLPTKLTPKLIGGKYVISA
                     KAQEFIKNSINQINEVDTEVDSLVDSILIAELKEKNKQIDHLQGVIEIQQKLLDQQQQ
                     LTLQANQQIDKLQKRLQLVYTEETSENESTTLSEETESIEKQTKKKQKWWKLWK"
     gene            3865..4035
                     /locus_tag="ACGE70_002512"
     CDS             3865..4035
                     /locus_tag="ACGE70_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694114.1"
                     /translation="MTRKKLIFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
     gene            4499..>4786
                     /locus_tag="ACGE70_002513"
     CDS             4499..>4786
                     /locus_tag="ACGE70_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HFF8694115.1"
                     /translation="MIGIFSIGITNTYASENSNFNINSQPIEEQAINFAKTDFEHSIT
                     SILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFSDADNTIKYIYTV"
BASE COUNT         1824 a          772 c          792 g         1398 t
ORIGIN      
        1 aaaaaaaata caaatggaga atattcagga agtatttcac aatttttagc aaaagaactt
       61 caaaatctag tagatagtaa tataacagat acaaccttat tttttcaaga taatagcatt
      121 tattattccg ataatggaca aattaaacta ctttggaaat cacctttaag tacagaaaaa
      181 aatggtgttt cttcctttac agaaaaaaat gatcttatat ccgtaaatat aaaaaataaa
      241 attaaaaatt tctctaaaaa acaaaatttt cctgcgatta aacgtgtaaa aagtgtctcc
      301 tccggagata actttattat gattaattgg cgtatagcag aaacccaaac aagttttccg
      361 tggtgtgctg cttacgtaga agctgctatc ttatgtaaca aaacagatgt acatgctact
      421 tcagctagag ccattatgca ttatacctat cctaatttaa acaacagtca aattgaatca
      481 aaaagtctta atcaagatca aataataaaa tatgcaaatt actgtggtgt ttatcctaaa
      541 aaagtagcac gaatactaaa cctttcagag gtccaaacac aactaagaaa aggaaatgcc
      601 atttatatgg gaactgctgg ttatggaaat tatagtaaca gccgtcatgc acttgcatta
      661 tttgggtggg tcaatcaagg tggttatcaa acttactatg tgtggaatcc ttggtataac
      721 tacccaactg taataagtgc aaatgcagga ccagttacaa ttcctgtacc tggaggtggt
      781 tttacttggg acaataccat tacaaattgg taaataaacc cattgcttta gttatgggtc
      841 tagtgttagt tcttttctta actagttgtt caaaaaaaca agaaatacaa aagactgatc
      901 tgattccttt tctaggaact tggcaaacta aagatcattc agaacgtcta gtaatcacta
      961 atttaaatac ttcaaaaaat atagaacaat ataccatctt agtaaaagag aacaatcata
     1021 aaattcaaat cattgataaa aatttaaata taaatgaaca agttttcatt tacaaagcaa
     1081 caaataattt actaacactg cgctctaata aaaaacaatt agattacact tttagtttgg
     1141 attctccaaa aaagctcgta tttattttga caacgaatga tgggacagaa gggacagcta
     1201 aaccaattga attttttaaa gctgattgat gaatagttgt ttgatattac aaaacaataa
     1261 tttcaccgaa ggtgaaacaa gcgaaacttt acaaaaagtg gctagcattt gctagccttt
     1321 tttgtattgt ggagcgtggg ttttgagggg gattcaaggg ggagcttgcg cccctttgaa
     1381 aagcggttat ttgcaccaaa tacgtaggta tttggctagc aaatatagag cttgccaaac
     1441 ccttttagtg agttttgtag agagtgacct tataggcact ctcacattac gaacgatacg
     1501 tccctcggac gtactgaaag ggtgggactt atgagcgaac taatgcaaaa tttggctagc
     1561 aaaaaagcga aaaaatatat gctaataatc gagcgaaatg aacacttttt caagtgagcg
     1621 aatccgccat gacaaagctg aaacagttta tttcagcttt gtcatggcgt gtgccgactt
     1681 ttttaaaagt ctaggcacac gagcaaatat ctttatcaag ctctaatttg cgttttaagg
     1741 cgtttcaaat gtctaagtat ataaatatat aagaatagag ttaagagtgc ttaaatcgaa
     1801 ttttagaggg aattcagaca attttagggt tgttagggct tgccctgacc gcctgtaagg
     1861 cgctttaaga acaagataag ggatttatac taagactgtc ttaaaacgtc tcaaacgaag
     1921 gctcatagct tctcaaagag ttccttatga acagttttag caaaggagcc ggaggcgact
     1981 gcgcagtata atgtgagagc agagcgatca caccttttta agcaactata gggagtaaga
     2041 ttcccagttt agaaaaatgc gcgaatctgt ttggagaaaa aagaagtggt tttttaggcg
     2101 ggaaattttg tccccctctt ttataatctc tttttaaacc tctttttaaa cctctttacc
     2161 gtcggctgta tccttactct cccaagggaa tgacgctttt atctacatcg ttttgtctgt
     2221 ttatctacat cgttttgtct gtttatctac atcgttttgt ctgtttatct acatcgtttt
     2281 gtctgtttgt acattgaatt agtacatcga cgatgttaat ataagatgta gataaacaga
     2341 cattttgtat ttcataacgt ctgtatctct tgtgcgagta agaaaggagc tattttaaat
     2401 atgtctaatg aaatcgtaaa gtatcatcat gaattaaata caattccgtt gcgaaaattt
     2461 acaccagtcg aaatgaattt gttcttctct atcgtatcaa gaatgcgtga tgtaggtgat
     2521 aaaaaagtac aatttacatt cgagcaatta aaagatttaa gcaattataa agcaactgca
     2581 aatgttcgat tcatagatga tttagaaaca acgtatgaca aattaatgga tttacgtttt
     2641 ggtagaagaa gtgctgacgg tttgcaacgt gaacgttttg ttttatttaa tcaatttaaa
     2701 atcgacggaa aagctgatat tccttttgct gaaatacaag ttcatgaaaa agccttaccc
     2761 ttacttaata atcttgaaga atgggtgcgc tattctctcc aacaattcaa tgaattagaa
     2821 agcagttatt caaaaacaat gtttcgatta ctaaaacaat acagaaccaa aggtttcgct
     2881 tatttttcaa aagaagactt tcatgaatta cttgatattc ctaaaagtta caaccaaggt
     2941 acgatcgatc aacgtgtgtt caatccaata cgacaagaac taaccgcaat ctttaaaggt
     3001 ttaacaatca aaaagaaata cggaaaaggc cgtgggaagc ctgtcattgg ctaccaattc
     3061 acatttaaac cagaaatcaa aaaagcagac gactttaaca aacatgcacc aaccaaagaa
     3121 gaacaaaatc gggctttaga actagctaat cgacaaactc aacacgaaaa agaaactact
     3181 tcattacaag aaagaattaa ggaacaaaag cgaaaattag aacttttaca acagttagaa
     3241 gagttagaaa aacaacaaaa aaatagagaa aacgaaacgg ataaacaaaa gttagacaac
     3301 aattccgata ttcctctaga actaagaaaa gacttacttg ataatcttca aaacttattt
     3361 aaagactaag gagtattcat gacagataaa tcaataaaag aatttgcaga agaactaggt
     3421 gtcagcaaac aagccattag aaagtatttt gataaactgc caaccaagtt gacacctaag
     3481 ttgataggtg gaaaatacgt aatatcagca aaagctcaag agtttataaa aaatagtatc
     3541 aaccaaataa atgaagttga tactgaagtt gacagtttag ttgatagtat tttaattgca
     3601 gaattaaaag aaaaaaacaa acaaattgat catttacaag gggtcataga aatacaacaa
     3661 aaattactcg accagcaaca acaactcacc ttgcaggcaa atcaacaaat cgacaaacta
     3721 caaaaacggt tacaattagt ttatacggaa gaaacttcag aaaatgaatc aactactctt
     3781 tctgaagaaa cagaatcgat agaaaaacaa acaaagaaga agcaaaaatg gtggaaacta
     3841 tggaaataac tggaggaaac attcatgaca agaaaaaagc tcatatttgg ttctatttta
     3901 ctaggaattc taggcttcag tccgatgtta ttgtcagtga tcaataaagg aaataaacat
     3961 ggtgaattga aattgaatcc aaattattac aacagtccta acgaagagtt atttgtaaaa
     4021 gaaacggata aataataaaa aaagactgct ctaacgagca gcccaaaatg agattatcct
     4081 agtttagcaa cataaaccat ttattagatg aaaatgagat acaaacaaag cgtatcaaat
     4141 cctctcaaaa ataacaagtc tttaaaaaat gacagataaa aatgcacaaa gaattagata
     4201 tagataaccg cgaaaaatca agctttagaa agttgctttt agctagacca attaaaaatc
     4261 acaaagtgag cgtctctatc cattggtaga ggggctactt ggcatagtgt acattatgtt
     4321 aactaacgct ataaataacg attatgttcc caaaacaata ttttatgaac tataacaaaa
     4381 atttgttatc gcatattgta tattttaatt ataggtgtat accacttaaa tttaacacaa
     4441 aggcgtgatt attttgaaac ccaaaaagaa aatttatcta ggtctcctct tttccttgat
     4501 gattggaatt tttagtatag gaattacaaa tacctatgct tcagaaaatt ctaatttcaa
     4561 tatcaactcc caaccaattg aagaacaagc aattaatttt gcgaaaactg attttgaaca
     4621 ctccataact tcaattcttt taatggaaaa taacaccgaa actgatatta actatactct
     4681 tggttcgcca tttaaaatgc tcgatggaga aaatacggca aatagttcta ttttctttcc
     4741 agtattttca gacgctgata atacgattaa atatatttat accgtc
//
LOCUS       DAXWOO010000175         4771 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.179, whole genome shotgun sequence.
ACCESSION   DAXWOO010000175 DAXWOO010000000
VERSION     DAXWOO010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.179"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(80..1888)
                     /gene="pepF"
                     /locus_tag="ACGE70_002514"
     CDS             complement(80..1888)
                     /gene="pepF"
                     /locus_tag="ACGE70_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFF8694116.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2064..3062)
                     /locus_tag="ACGE70_002515"
     CDS             complement(2064..3062)
                     /locus_tag="ACGE70_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFF8694117.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3288..3941)
                     /locus_tag="ACGE70_002516"
     CDS             complement(3288..3941)
                     /locus_tag="ACGE70_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFF8694118.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4273..4671)
                     /gene="spxA"
                     /locus_tag="ACGE70_002517"
     CDS             complement(4273..4671)
                     /gene="spxA"
                     /locus_tag="ACGE70_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFF8694119.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1401 a         1003 c          744 g         1623 t
ORIGIN      
        1 aaaaaatacg tcgactccct tacaagagag ccgacgtatt tttttcgttt ctattcgaga
       61 aaatcaaaaa tagaaagcct tataaacgat cgatcaattc ttctaattca tttagacgtt
      121 gttcaaacat cgacattgca tcttcaatat atgcagcctg tgtcatatca actcctgctt
      181 ttttcattac ttccacagga taatcgctgt tacctgcttt taggtaagcc aagtagttct
      241 ctagtgcctc tggttcctgg tttagtattt tcttcgccag tgctgaagca gcagaaaagc
      301 cagtcgagta ttggaaaaca tagtaattat aataaaaatg cggtatccgt gaccattcga
      361 atttaatttc tgggtcttct tcgactgctg gaccataata ttttgcattc aatttaccat
      421 aactatcact taggtattca ctagtcaatg gcacaccttt ttcatcttcc gtatgcatga
      481 aatgctcgaa ttcagcgaat tgtgtctggc ggaaaactgt tcctttaaac ccatccaagt
      541 aatggttgag tacataagcc cgtacacgag ggtctttttc tgtttccaat aaatactccg
      601 ttaggatatt ttcattcgtt gtcgaagcaa tttctgctaa aaagatggag tagtcgccgt
      661 acacataagg ctggttcgaa cgagtgaaat aactatgaac actatgtccc atttcgtgga
      721 caagcgtaaa tagctgatcc agcgtatcat gccaattcaa taaaatatat ggatttgtgt
      781 catagcttcc cgaagaataa gcaccgcttc gtttcccttt attttcgaca acatcgatcc
      841 aacgttcaga gaatgctttt tctacgatgg ccatgtattc ttcacccatt ggtttcagtg
      901 cttctaaagc tttttctttt gcttcttcgt acgtaaaggt aattggagct tcccctaaga
      961 ccggtgtata aagatcatac atgtgcagtt tttccacttc taataaacgt ttccgtaatt
     1021 ccatgtatcg atgtaacaaa ggcaaatgct tgtttaccac gtctaccaaa gtatcgtata
     1081 cactttcagg aatatgatta ttgctcaaag acgcttctct ggcagagctg taattacgga
     1141 ctttcgcttt aaaattatgt ccttttatat gtgtgcctaa agtagaagca aatgtatttc
     1201 taaattgttc gtaaacactg tacaacccct taaatgctgc ttcgcgcacc cttcgatccg
     1261 tgctttctag caactgacca tacacgccat gagataattg gactatttca ccattttctc
     1321 cttcaatcgt tggaaaaact agatctgcat tattcaaaac agcaaatgta tcacttgaag
     1381 catcaaagat ttcacctgct ccagcaagaa gagattcctg ctcagcagat aagacatggg
     1441 ctcgattatc tactatttgc tggatataat ggcgatagac ttccaatttt ggttcttctt
     1501 tgaaatattg ccaaatctgg tcatctgaca attgcaatat ttctggttca aaccaggaaa
     1561 cggcttcact aactttcgaa aacagactgc ttgctcttgc ataaagcgct tggtaatctg
     1621 tatttccagt gtcttgatcg tttttaaggt gcgcatatac ataaatgact tcagtttggc
     1681 gataaaccct caatacgaat tcaatcgcat ttaaaaattg agaagcgcct tgatcaagtg
     1741 ttcctttgtg tttttcagat tgttttaact cttctgataa ttccaaatat ttctcatcaa
     1801 actcttggtc gcttgaaaag atcttggtca agtcccaagt taaattttca ggcaattctt
     1861 ctcgttttgg caactgcttt acttccatga aatccactcc ttttcttccc ttagctctat
     1921 ctatcaaaat agatcatagt ctaacatcca catgtcacag catctaactt ttctactcat
     1981 tcatgtgtct gcttccatca attttagcat aactaaatct tgttttacat agaaaaccga
     2041 ttcctagcct atttttatac ttactatttt ttcaaagaag acaagtgtat gcattcgtta
     2101 aaaaactgct gatagaccat ctcttcatca atcataggaa aaagccattc ccttttatgt
     2161 tcttttattt ggcaaagcca tgagttgaaa tcaggaactt tctggttttc caaggcttct
     2221 tcgtacaaca ttcgataaag aaaaacctgt tcttgtaaat aaaagaaaaa ccgacttttt
     2281 tgatagatcc acgatgatag atgcaaaaga tgtttatgac gcaagtagca ttgttcttgt
     2341 atggatactg tattcttttg cttgtgaata agttggcgtg tcagccattt cttcccatct
     2401 tcactcactt tttctatttt tttcatttgg ttcattttaa tttcgttact agtgaatatt
     2461 tctttaaggt ccctactaga aaaagtccag cagctactgc aaaaggaaat atgccctgat
     2521 acagtctctg taatatgatg atacaaataa agcttttgtt tcttgagatc agctttccag
     2581 caatgcactc ctctttttct attatagtag cagcaacttt tttctatcaa tgagaattgc
     2641 gattgtccaa gaaaagaatt accaagtatc caccatactt ggtaactgtt attcaaataa
     2701 ttttgggttc tttcaataaa tctctggtga gacagtctag aacattggat ttctattgct
     2761 agattcgcca acaaaatatc cggcctttgc cgcaacctcg gcaaataggc ttccaaaaat
     2821 actggttcta ctgactgtcc cagccaatcc atgaagcatt cctttaactg taagtgctct
     2881 tttgattctc tttcactaaa gaccgaacag ttatctgatt tttgatgggc aaaatgagca
     2941 cattttttct ttcccttttt caaaatcact ctttttcgac agccacgaca ataatatttc
     3001 tttccttttt ctgcatgttt agcaaaacag cacttattat attcattcat tgcaagcagc
     3061 atttttctcc ctctgaactt atttacgtct aagaatttat ttacacgaaa aaagagggcg
     3121 tgaaaaagct attctgcatc aagtaataag aacagctaaa caaaaatagt tcccaatatt
     3181 tttcgtttgg ttgggcttgt taccgcagat gcaagctttt gaacacattt atacgaaaaa
     3241 aagactgcga cacaattttt gtcacagtct cttactataa tgctgattta tacaaaataa
     3301 aatttcgttc gctcgatagc attacgcgcc atcagacatt gtccatattc attcaatgtt
     3361 tctggtgtac gatcgctctt ttcagcaaac tccaaaattc gagcaatctg attctctaca
     3421 tccgcttggt tgagattttc catccagaag tgaacgacta ggtaatagcg ctcttcgaat
     3481 aaatacaaat cagtccaagc agactctagt tcgacatctg ttgcaagttg gatcacttgt
     3541 tcaaaatctt caaaagcaaa agttgcttgt ctttcctgtt cttctagggc gcttccagat
     3601 aattcttcct ccggatcgct gccaataatc tgctgacgaa gaaagtcact cacatcttct
     3661 gaatcaacag tagaatcttc tgattgagaa atttcttcat ccggaagatt tttactgata
     3721 aacagttcta atccgtctcc ttttggcaaa acttggaaag tgacagcttc gcttcctcgg
     3781 aactcctctt cgatatcaac ttcttccaaa atactataaa agaaattttc gatttctttg
     3841 tgattgccaa gtaaatccaa aaatgaaacg ccccgttcct ctaaatcttc atgaccaatt
     3901 agtacacgga tcgtattttc attaatatgt tccatttcca ttttgctaca cctcactttg
     3961 ccacaatgtt taatatagat tcattgtagc gaaatcagct taaatgtaaa gaaaaaaaat
     4021 ctcgctgtta agcgaattta ataaaaccag cggatttttc aacctttcaa gctatcttat
     4081 ttaactataa catttttggc agacgaatac tatatagccg acttttcagc taattttccc
     4141 tctctatttt tcgtgcaatc atcatcttct actacctttt tatacatcta atcaagaata
     4201 ctttttgtaa aaacaaaaaa acttggaaaa actatctttg taatagtctt ttccaagttt
     4261 cgtattatcc ttctataatc cagccatcaa ttgtgcttga cgtaattcta attgtcgtac
     4321 atcgcgcggt aggaaccgac gaatttcatc ttcattaaaa ccaacttgca gacgtttatc
     4381 atcaatcatg attggacggc gaagcaatcc aggattgttt tgcacaagtt ccaataattc
     4441 ttgtaacggc agatcatcta aatccatgtt cagtttttgg aatactttgg aacgtgtcga
     4501 gatgatttct tctgttccgt cttctgtcat ttgtaaaatc gcttttaatt cagaactgtt
     4561 caatggttct gaaaaaatat tacgttcaac aaatggaatc tgatgttctt gcaaccacgc
     4621 acgagccttt ctgcaggaag tacaacttgg ggaagtatat aatgttaaca acgtgcacca
     4681 ctccttttcg tgataagaat cacttttttg taacagaaga tcataataat ctcccatctg
     4741 tttctattat acataatcaa cttttaaata g
//
LOCUS       DAXWOO010000176         4768 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.180, whole genome shotgun sequence.
ACCESSION   DAXWOO010000176 DAXWOO010000000
VERSION     DAXWOO010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4768
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.180"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(227..314)
                     /locus_tag="ACGE70_002518"
     tRNA            complement(227..314)
                     /locus_tag="ACGE70_002518"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(280..282),aa:Ser,seq:cga)
     gene            complement(459..1025)
                     /locus_tag="ACGE70_002519"
     CDS             complement(459..1025)
                     /locus_tag="ACGE70_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFF8694120.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            1280..2611
                     /locus_tag="ACGE70_002520"
     CDS             1280..2611
                     /locus_tag="ACGE70_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HFF8694121.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGVFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSVLSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGILLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLSGIREIFDSSGKLTT
                     DFIANSGIGATLINMGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2577..2927
                     /locus_tag="ACGE70_002521"
     CDS             2577..2927
                     /locus_tag="ACGE70_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694122.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(3019..3438)
                     /locus_tag="ACGE70_002522"
     CDS             complement(3019..3438)
                     /locus_tag="ACGE70_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFF8694123.1"
                     /translation="MMTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHTDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(3439..3783)
                     /locus_tag="ACGE70_002523"
     CDS             complement(3439..3783)
                     /locus_tag="ACGE70_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFF8694124.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVEWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            4133..4729
                     /locus_tag="ACGE70_002524"
     CDS             4133..4729
                     /locus_tag="ACGE70_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFF8694125.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1299 a          884 c          835 g         1750 t
ORIGIN      
        1 tagtactcag ggggaatcaa aaaatattgt aggttaaaag tacaacaata aggggctgtg
       61 acaaaagtct tgtcacagcc ccttattccg aacaaacggt gttcaaaagc ttgcatctgc
      121 ggtaataagc ccaaccaaac gaaaaaatag gaaaagctat ttttgtttgg ccgttcttat
      181 tacttgatgc aggacagctt cttcacaacc tctaatcgtt gatataagga aggtacagga
      241 tttgaacctg cgcgccggta ttagccggtt cgccggattt cgagtccggt gcattaccac
      301 tctgccaacc ttccgtaagt actattttta aacttttttc acgaaatgtc aagcctattt
      361 ctaatagata aataacagaa atcgcttttt ttagcaaaaa agtccattga cagtttgcag
      421 ccaacggact taccgatcaa attcatactg atagaacact atctgattat ttcaggatta
      481 tctaatgcta aaatcgagtc aattgtccgt ttgatcagtg caatgatttg ttcttctgtt
      541 ggatgcatga tgtttgatga catcaatgac gctaagccag tcactgctac ccatgtacca
      601 gtatgcagag catcgacata ttcatccgat aattgattgt atctttcatc ttctttaact
      661 atttcacgga aataattgta tgaaaaatca tgcattttct ttccgtcccc atgttcatca
      721 atgtacaatg ccatgtaaag actatgatgc ttttttgcaa agtagatata gttcaaaccg
      781 agatcaatga ttttatcacc agtacgttca atagggaaaa catcttcttt tagttgttca
      841 aagatagaat ccagcaacgt attttttaaa tcttccatgt ttttgaattc cagataaatc
      901 ggctgtgtcg aaatccccat ctttttagca atatttctcg cagtaaatcc tttaaaccct
      961 tcctttgcaa caacatcata agcagctttt agaatttgat ccttggtata gacttttctt
     1021 ctcattaaaa ctcccccact tctttactgc agtatatgtt gttttattgt accctatttt
     1081 tagcattttt tgcaaacatt ttactcaaaa cgttatattt taaatttttt tcagtcttct
     1141 ttaacttttt tttgcaaaat taaaaaaatt tggttaaatt caatgggttc ccttacattt
     1201 ttagttttga cctaaatgat agttgtccaa atctttcatt tcaagttata gttaaagaaa
     1261 atgaaaaaga aggtgtgtaa tggaaaacga aactgacgtt atttatattc atcctcaaaa
     1321 acgaatcgtt tcacagaaaa gaaagtattt ttatcttggt tttacaggtg ttttttttct
     1381 ttttattggc ttgctttcta atactcctac tgataattgg agtggattgc taactattct
     1441 tacctcgcca agcaatcttt tgacagatta ttttgcttta ggtgttttcg gaagcgcatt
     1501 cataaatgta gggattttga ccttactttc tgtgctttta gcttatcggc acaaggtgat
     1561 tttaaacggt cccttatttg cttcgattct aacagttacc ggtttctctt tttttggcaa
     1621 aaacctttat aattctatct caatcatttt aggtgtgtat ctatacgctg tatttgtcaa
     1681 taaacctttt tctcagtaca tcatgatcgg actgtttggt agcgtattat caccggtcgt
     1741 tagttatatc acttttggta tgcgttttcc tttactgata ggtattttat tggggaattt
     1801 agctggcata gcaattggta tacttcttcc gcccttagct gctcaaactt tagttttcca
     1861 tcgtggattc acactttata atattggatt cacctctggg ttgattgcga tgacttttac
     1921 tgctgttttg aggttattca gctattcaat cgtcgaaaat acattggttt tcaatgaata
     1981 tcactttcct ctcatttgga ttatttttgg ctttttttcg ctgacagtcg gaattggttt
     2041 ctattataat tcctttcggc tttcggggat tcgagaaatt ttcgattcat ctggaaaatt
     2101 gacaactgat ttcattgcta attctggtat tggtgctact ttgatcaaca tgggcttagt
     2161 tggtctgatg ctgtcttctt atgttttgct ggttggcggt cagttaaatg gacctgtcat
     2221 cggtgctatt ttatctgcgg tgggtttcag tgcttttggt tgtcatttaa aaaacagttt
     2281 tccgatacta gtcggaatat ttattgcttc tttgtttgga acttttcatg agatcaccag
     2341 tactggcatg cttgttgcag ctgtgtttgg gacaggtctt gcgcccataa gcggttttta
     2401 cggcagtttt tacggtgtaa tagctggtgt gctacatatt gctcttgtcc ataacgtcag
     2461 cactttgcac ggaggactaa atctatataa cagtggtttt tcaactggtt tcgttgcagg
     2521 tatccttgta ccgatactag ataactttac cgctgtcaga aaggaaaaaa agacacttgg
     2581 aaaaagaatt atcaaaaaga atcatcgatg agcttcttac ttatttatat tcccatgaaa
     2641 taactgaaat acgtattgga gtagaatttg ctgaggaagg gcttttcata gagatacagg
     2701 gtacggcaga aactcagcca gaagatttag atgtacttgc tgatttgctg aacaatcctc
     2761 gggaccctag tatggaaatc tactatcaag aactattagg taatccccga catgatcggg
     2821 aagattatca tctgttaggt agtttgattg atgacgtgga aatcatgtat gcccagccga
     2881 ttatcaatat taagatcttt cgacgtgctt ctcattccta ttattaaccc tattttctat
     2941 ttataaaaga ctgaatcaaa aaagcagctc ctgaaagttc gattaaaatc tactttcagg
     3001 agctgttcaa gcttgacatt attgattttc caatccttca tacacttcat ctgttacagg
     3061 ttctaaaaac tctgaattac cagcagtgat cgccaaatgt tcaaaccaac tgtcttttgt
     3121 cgcaccatgc caatgtttaa tccctttgtg cgttacgacg acatctccag ccattaatcg
     3181 tcttgctggc tttccttctt cctgatacca gccttctcct ccggtcacaa gtaagatttg
     3241 atagccgtct gtatggacat gccagtgatt gcgacaacct ggttcaaaag ttacattacc
     3301 tacaccaaca gaaacttcgg gatcattcac taacatattc agatagcttt gcccggtaaa
     3361 tacttgagcg taagcttcgt ttttttcacc tgctggaaaa atcacgccat tgtgtatttc
     3421 ttcgtgtttt gtcatcattt acgcctcttt cttatcttcg ctattttctt ctaaatagat
     3481 ttcttttgct aaactaaaag ctgaccaagc ttttggccat tctacataaa aagctaaatg
     3541 ggtaatgatt tctgctattt cttcactagt aatgccattt tgcttgccaa tcttcagatg
     3601 agctggcaat tgttcaaaat ttcctcctga aattaatgca gatatggtga tgatactacg
     3661 ctgatgagca gataattctt ctctggacca tacttgtcca aataaaacat catcatttaa
     3721 ttctgcaaat tttggtgcaa aatctcctaa ttgatctcgt cctgcggttt gtttttctgc
     3781 catttctctt attctccctt cacgactgtt aggtgattca actatggttc taaggcaact
     3841 ttattttcta ttgtttcttc gcatttcatt ttaaatgtag tctttcagcc tgtccaatgc
     3901 ttagtttatt cagtcgatat gctttttaag catagttgct aacgcttttt ttattttaaa
     3961 aaaaacaagt tattaaataa gtctttagtt ggatgttttt ttgagatcaa tcttttatac
     4021 ttatgatgaa cttacaaaat tgtttgtttt ctgtcatatc aaaaaatggc gcagttagtt
     4081 gcttttctag taaagtatag ttaacaaaga acctctaagg agttgatgaa taatgaatat
     4141 tgctgcctct cgaaaactga taaatttcat atccattatc ggattaggcc tatcaatcgc
     4201 gctgacgatt tattttatta atttaggtgt tttcaaagat ttgaatgcgt taagaggatt
     4261 agtaggtgat tccatcattt taggaccgat tatttttgtc ttcatccaaa tcctgcaagt
     4321 agttattcca atcattcctg gagggatcag tactgcagct ggtgtcctga tatttggccc
     4381 ttatgctgga tttatctata actatattgg aatctgcatt ggttcgatta tcatttttct
     4441 gctagggcgt cgttatggaa agccattcat actttccatg attagcgata agacatataa
     4501 caaatatatt ggctggctgg ataatcaaaa ccgttttgag aagctgtttg ctctagctat
     4561 ttttcttcca gttgctcctg acgatgcgtt atgtttgatg gctggattga caaacatgtc
     4621 agtgaaacga tatacactaa tcattttgat tgctaaacca ctgtcgattt tcttatatag
     4681 tatggccttg atttatggag gtcaatatct aagtggtctt cttggataat atttattttg
     4741 taaaattagt cattgattga caatgtta
//
LOCUS       DAXWOO010000177         4728 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.181, whole genome shotgun sequence.
ACCESSION   DAXWOO010000177 DAXWOO010000000
VERSION     DAXWOO010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4728
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.181"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            63..254
                     /locus_tag="ACGE70_002525"
     CDS             63..254
                     /locus_tag="ACGE70_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694126.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
     gene            complement(628..1440)
                     /locus_tag="ACGE70_002526"
     CDS             complement(628..1440)
                     /locus_tag="ACGE70_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8694127.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            1603..1710
                     /locus_tag="ACGE70_002527"
     CDS             1603..1710
                     /locus_tag="ACGE70_002527"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8694128.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            1780..2835
                     /locus_tag="ACGE70_002528"
     CDS             1780..2835
                     /locus_tag="ACGE70_002528"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFF8694129.1"
                     /translation="MQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERFEELD
                     YAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQFFRK
                     VLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINNESNG
                     ETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAKIISK
                     THDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQSFEKT
                     MSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYYQEEV
                     EYYERKLQSISNEKTSY"
     gene            2977..3180
                     /locus_tag="ACGE70_002529"
     CDS             2977..3180
                     /locus_tag="ACGE70_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFF8694130.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            3240..4442
                     /locus_tag="ACGE70_002530"
     CDS             3240..4442
                     /locus_tag="ACGE70_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFF8694131.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
BASE COUNT         1587 a          745 c          985 g         1411 t
ORIGIN      
        1 attagagaaa aataatttga gaggtagata gtttttattg gaggaagtaa ttatgtatat
       61 ttttgaaaca aaaatttttc attgcaataa ttgcaacaaa gaaatttcat cgggggaaga
      121 ggtttggaca aaaatggaaa tttccgccaa aagtaactgc tcctcaactg aaatcaatta
      181 aagaactaga atttgagaat gcactggtaa tttgtatgac atgtactgat gaagtatttc
      241 gaaaacattt ttaaaggttg agatgtatat agatttaacc aatcatttat ctctctcgga
      301 aaaggaatat taactaacca gcgggttatc aaaaataggt aggaaaactg tacttaatgg
      361 cttatttcgc gctgattatg agaatatcag tctgtttgat taatttttag ggttcaaaat
      421 acagagacaa actccaggtc gggtttcgcc acaagcgaat tacccgatct ggagttttgt
      481 ctattgcagg aaggcgtagc cggactgcag gaataaagcc cccggttcta acagggggct
      541 aacaaagaac aggaaactgt gacaactacc cattggggag tgtcctagca gtattttctt
      601 tgtcacgtgc ttatcaattt tgtcacctca ttttgatgat ggatacgctg catttaacag
      661 gcgttctttc tcgacttcca tcacgaatgg tagataaaga tattgctgtt ctagtagata
      721 ttgattgtcc tctgtgtact gataagaagc ggtttcttct gaatagacca acggggcaat
      781 tcgcacatcg ctggtcaatt gattaattga gtatagaagg atggctttct cacctagttc
      841 cttgggtaaa tgtttcccaa aataggaata ggatttttga tttgctaaat agacgagtat
      901 gcgatccaca ctaaagttgg agaaggtaaa gggcatcatg tgcagaatat gattttgatt
      961 atcatgttgc aaatagagct gggcttcctt accaaaatta gttgaaccaa gataaagctg
     1021 ctttttttca tcgatacatt ggaaagtaaa ctggtcttgt tcagttggtg gaatgagatt
     1081 tgtaataaaa aattcaatca ttcgatccat ttctttttca ctaacaaggt cagcgtgttt
     1141 gatttcgtct actatgagac cttcaatctt aggatcaagc ttacctgcct ttttttggag
     1201 catttctaaa atgtattgat tctcttgatc accatacaaa agatattcat tggtgacatg
     1261 acctatctct gcgatttggt gaagggtttc ggtttttgga aggttgatcc cacgttccca
     1321 gttattgact gtacttcgcg gtaaatcacc aattagtttg ccaaatttct ccatactcaa
     1381 ctttaattct tggcgaatct gccgaattcg attgccgact gcttgcttgt ctatctccat
     1441 gaaaactcct cctcaggata agttcttaaa tttagtatac gtgtgtactt aaaaaaagtc
     1501 aaaacatatt gacttaaaat aaatacaggc gtacaataaa aatgtactta aaataagttc
     1561 aataggttct ttgacaagta aatagcagaa ggaggtttgt cgatgaacca agttaatcaa
     1621 catgatctcg gtgaagccat tcgagtttct cgagaagaaa gggggtggac acaacgatat
     1681 ttagcagaaa aagtggggat ttcgagatag ttattatcga aagtagaaaa aggaacgaga
     1741 caattaagtg aagaaaaatt gaatcttgtg ttggatagtt tgcaagaaga ggtcgttcca
     1801 gttaaccgtg ttttgattga ttatctaacc attcactttt tctcgaatca acatttaaag
     1861 ctaattgaag agattattga aatgcccatt gaacgttttg aggaactgga ttatgcgcca
     1921 aagggctata ttggccaata cgtgtggaat caagtaatca cgattcggta ttccattgac
     1981 gatgcggtta aaggaactgt gatggagttt tcaggtcaag gctgtaaaca cttagcaaga
     2041 tggctaaaaa cacgaaaaac aaattggcaa cagttttttc gtaaggtatt agattatcag
     2101 gggaacttta cacggattga ttttacttta gatgattttg tggggagtct cagcattcca
     2161 gaacttaagc ataaggtaat actaggccat gtgtggacca cttttcaagt gagtgaatct
     2221 catggcggta cggatatcat aaacaatgaa tcaaatggtg agaccttgta tttaggctca
     2281 aagaagagtc agtgtcgttt ttgcttttat cagaaggacc atgaacaacg gaagcgacga
     2341 ggaattcctt tagaagaggc aaaagtaaag aatcgctttg aactacggta tcgaaaagag
     2401 aaagcgcaaa gcttggcaaa gattatttca aaaacccatg atttaaccaa actgttcttt
     2461 gagttactta atggagcaat ttgtttttat gaccgtgatc caaatgatct aggtgcaaaa
     2521 gttgatgcca aatgggcagc ctttattagc aattatggcg caatcaccat ttctttagat
     2581 acaattcctc aaagttttga gaaaactatg agttggttaa ttcatggtgt ttcaccatca
     2641 ttagttttta ttgcacaggt cgatcaaacg tttcattctt ccttgttgcc tttgattatt
     2701 gaacaaggag aaatgaatcc aagacagatc aaacttctcc aaaatatgaa ggcagacccg
     2761 gagtattatc aggaggaggt tgagtattat gaacgaaaat tacaaagtat atcaaatgag
     2821 aaaacaagct actaaaaaag caaagtactg cttacacaac aagttgtgca acgtactccg
     2881 cctcgacaag acgagtataa cacaaccatc gaatgagaaa caatagatta taaagaggat
     2941 tattttaaaa ggagtaaaga atcaggaggt gagagaatgg aaacaaaatg tgatttcatg
     3001 gtaaatcgtg caattttgat agagatgggc tttaaaccta gtcaagctgc acgaatgatt
     3061 aaagagagca aggcatatct tgcaaggatt gaaggtattg atttttataa taatcgccaa
     3121 gttggtgtgg tgccatcccg agtaattgaa catctttttc acattcaagt tgcggaataa
     3181 acagtacaag cgcggtacta ttaaagtagc ttttctcaag tacagggagg aatggaacga
     3241 tgccaaaaat taaaaatatt taccaagata aaaagacaaa aaaatggttc ttccgagcgt
     3301 atctaggtat agatgaaaat ggaagaaagg ttcagaagac taaacgaggt tatgcttcac
     3361 aaaaagatgc caaaattgct tatgacaagt acatggaaac acatgatttt aacaagacaa
     3421 ttcctaatca actgtgttct accaatcaaa tgactttcga agagttttac aaggataggt
     3481 ttgtaaaatg gtacgagaga caggtcaaaa gtcaaacata tgagaatgcg caattcattt
     3541 tcgaaaagcg tatgcagttt ttctatcatt ttcgtgttcg agatattaca agtcacgata
     3601 ttgaggattg gttatttgaa ctaagccaga cagagtcacg taattcacgg aaaaaagtta
     3661 atggcgaaac gttatcaaaa tcttatatca atcggattcg agggcaccta aaaattgtat
     3721 tggatcgggc tgttaaagag ggggtgattg ctaaaaatcc cgttgaagat gtttcttcgc
     3781 ttccttctga gaataagaaa gttgagtttt gggaacacag tgagtttcaa aaagtgatga
     3841 atgaattgaa ggatgataag attcaaaccc accatcggaa gattgtgtat gaaatgctct
     3901 tttatactgg cattcggatt ggagaacttg aagctttgtc ttggtcgaat gtagattttg
     3961 agaaaaacac gataacgatt gagaagacat tgatttataa tacaaaagat gattggtact
     4021 tttcgactcc caaaactaaa tgtgcttatc gaacgattgg gttggggaag aagctttcct
     4081 taaaactcag acagtggtat gacttgcaaa aacgtattgg aaattttgaa tatgttgctc
     4141 agttggacgg aacatttacg ccaccttatt cttttgcgaa ctggttaaaa gaagctgcta
     4201 aaaaagcggg tgtcaaaccg attaagcttc acgcgctacg acattctcat gttgcatttc
     4261 taattgagca gaatattcaa ccgctatcta ttcaagaacg attgggccat gcgaatattc
     4321 aaatcacttt aggaacttac ggtcacttat atgcaaagtc agatcaacaa gtggtaagtg
     4381 cgattgataa tgttcaagag ggcgctattc tttcaaaaaa tgacaattta ttaccagtct
     4441 gagttttcca cggttctacc ataagacaaa taaatgcttt tgcaccagtg ttttcagtcg
     4501 aattgctcct gttcaagatg tatcggaaat agcgagaaaa taagcaaatt tgcataacta
     4561 taataaaaag gacttagttt tgatgggaaa tttttcaaaa ttaagtcctt ttttatgtgg
     4621 ttgaaacggg tatgatcatt ttaaggaagt gatctaagat gaaaggacga ctgaacaatt
     4681 atcatattag aaaagcttta ttgtattcca caaacaaatt actaagaa
//
LOCUS       DAXWOO010000178         4708 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.182, whole genome shotgun sequence.
ACCESSION   DAXWOO010000178 DAXWOO010000000
VERSION     DAXWOO010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4708
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.182"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(29..1177)
                     /gene="nagA"
                     /locus_tag="ACGE70_002531"
     CDS             complement(29..1177)
                     /gene="nagA"
                     /locus_tag="ACGE70_002531"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFF8694132.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1253..2089)
                     /locus_tag="ACGE70_002532"
     CDS             complement(1253..2089)
                     /locus_tag="ACGE70_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8694133.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2273..3490)
                     /locus_tag="ACGE70_002533"
     CDS             complement(2273..3490)
                     /locus_tag="ACGE70_002533"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFF8694134.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3639..3800)
                     /locus_tag="ACGE70_002534"
     CDS             complement(3639..3800)
                     /locus_tag="ACGE70_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694135.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            3873..3956
                     /locus_tag="ACGE70_002535"
     tRNA            3873..3956
                     /locus_tag="ACGE70_002535"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3907..3909,aa:Leu,seq:gag)
     gene            complement(4196..>4708)
                     /locus_tag="ACGE70_002536"
     CDS             complement(4196..>4708)
                     /locus_tag="ACGE70_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFF8694136.1"
                     /translation="YYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNL
                     NNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFC
                     SRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGI
                     CTFIITVINR"
BASE COUNT         1413 a          989 c          763 g         1543 t
ORIGIN      
        1 tttagtttta aggttaactt aaattatatt atgcgtggaa acgttccacg ccatctagat
       61 aagttgcttc caatgtcata tcagggttca atacgataaa gtctgcatcg cgtccttgag
      121 cgatttttcc acatgtatca tcgattttac agctgattgc tggaatcaga ctagccatca
      181 tgactgcttg ttctggtgtt gctaatcccc agtctacaac atttttaatt gcttctttca
      241 atttcaatat acttccagct aagttgccag attctagacg tgctgttcct tcttttacta
      301 ctactggaaa ttcgcctaaa ttgtaatttc catcaggcat cccaccggcc atcatacagt
      361 ctgtaattaa tgcgacatga tcatgtcctg ctttttccat taggattccc gcagcatttg
      421 gatgtacatg atggccatca caaatcaatt cagaaaagac atgttctaaa gataataacg
      481 cacccaccat acctggttcg cgatgattca atccacgcat accattgaaa gcatggacaa
      541 atacacttgc gcctgcttca acagcttttt gcgcttcttc tagtgtagca ttactgtgtc
      601 ctagagaaac aactactcct tcttcagtca cttgtttgac aaactcttct acaccttgac
      661 gttccggtgc taaagcaatt tttttgatta aaccacctga agcttcttgc cattcatgga
      721 aagtatccaa atcaggatcc ccgaaataac ttgggttttg tgcccctttg tgttcttctg
      781 tgaagaaagg accttcaaaa tagattcctt tgattttagc tcctggtgct tcctgataca
      841 ttttgccgat tgtctcagct acatctttta aacgttcttt gcttgacgtc aatgttgttg
      901 gtaaaaatga agtcactcca caagataaca gaccttcaga catcactttg atgccttctg
      961 catcattatc cattacatca tgattcatat agccgtgaat atgtgtatct accaaaccag
     1021 gagcaatcca tttgccgctt tggtcaatga tgttgacttc tccctcaggt agttctttgg
     1081 tgtattcacc aaaaagtcca tcggtaattt caagatagcc tgcatgtttg acacctgacg
     1141 ctaaaaagaa tttgtcagca tagataaaag ttctcattct attctctccc tttgtttttc
     1201 tacctctaaa gtactcttat ttagagaaga agtcaagaat ggtctacacc atttacaaat
     1261 tttttgaaat ttgtttcaca gcttgtgcta agccttcata gaatgctgct ctttctggtt
     1321 cttctttacg cttgtatatt aagcttttaa gcacaaataa atcattgagg taatagtagc
     1381 tattcttctc ttgagcccaa tagattcctt gattcacctg ttctaacgct tcgtcgattt
     1441 cattttgatg gaataagaaa tttccataat tataaaaaat ttgcgaaaat tcactttttg
     1501 atcgttcatc agctgattga tgcagcagtt ggatgcttcg actcaaatag tagcgtgctt
     1561 cagccgtttt tcctgccaca ccatatattt ttccgataca actaatcagt tgtatttctg
     1621 tgtcagaaac atggattctt tctttttggc tagagtagct taatgcttct ttcaaataat
     1681 aaagggactg agagatattt tgacgcaacg tgaattcgca aacaccgaga taataataat
     1741 agcgttgaaa gtcagttgct tgatacagat tttcagaaat atcagaagat tttagtactt
     1801 gttctaactt ccgataatca cgtttttgga aacaactatc caacagttca aataatttat
     1861 cgtttgtccg agtagttttt cgagagagat tcatcacatg atcaatcgtt acccctagcc
     1921 gttgacaaat ctgcatcatc accaatacat tcggcagttc ctcattattt tcgatccgac
     1981 ttaatacact ttgtgaacaa atatcatgcg aaagcatttt ttgcgtcatt ttttgttctt
     2041 ttcgaatctc tttaatgatc gttccaaagg actcgattaa cgtttccata agtatcctcc
     2101 ccaaaatatg cgtattcgca ttctattcgt attttacttt aggaagtcgc aaacttcaag
     2161 aaaagacttg caactaaata atatacttta gttatttaag aaaactccac tagaaagaga
     2221 gcctcagtgt gtttgtctgt ggccctccta ctgaaaaatt tggtttttaa aatcattttt
     2281 ggcgataggt tgctaggaat tctctcattt tcttagctgt ttgtttcaaa tcatccattt
     2341 taggaagata aacgattcga aaatgatctg gctggttcca attaaatcca ccaccatgta
     2401 caagtagaat atgatgttca tgtaaaaagt ccaaaacgaa tttttcatca tccaaaatat
     2461 tgaatcgctt tgtatcaatt tttgggaata tataaaaagc agcttttggt tttacggctg
     2521 aaagaccagg aatatcattg attgcattat agatatattc tctttgttca tagatccttc
     2581 cgcctggcag gagcagctca tcgacacttt gataaccgcc tagcgcggtt tggataattt
     2641 gctgagacaa tacattggaa cacaaacgca tggaggacaa catattcagc ccttctatat
     2701 agcctttgac gtgtgactta tctccactta ggaccatcca gcctactcga aatcctgcta
     2761 cacgatgaga tttggataga ccgttcagcg taacaacgaa caaatcaggc gctaatgtag
     2821 caatcggaat atgttctaga ccatccatca ctaagcgatc atagatttcg tcggaataaa
     2881 taatcaggtt attttgtcga gctacttcaa cgatttcttc taataattct tttggataaa
     2941 gtgccccagt aggattatta ggattaatga taacaatagc ttttgtccga ctcgttattt
     3001 ttgatttgat atcatcaata tcaggatacc attctgcctc ttcatcgcaa atataatgaa
     3061 caggtgtgcc tccagctaat gcaatagaag ccgtccacaa cggatagtcc ggcattggaa
     3121 ccagtacttc atctccattg tccagcaacc cctgcataca catcgtgatc aattcgctca
     3181 ctccgttgcc ggtgtagata tcattgatcg tcacattcgg aaaatgtttc agttggcaat
     3241 attgctcaat tgcttttcta gcagaaaaga tccccttgga atctgagtag ccttctgagt
     3301 cacgggcatt catgatcaaa tcacgaatga cttcgtttgg tgcttcaaat ccaaaaggtg
     3361 cgggatttcc cgtgttcaat tttaaaatac taatgccctc ttcttgcatc cgctccgctt
     3421 cttctagaac gggaccgcga acatcgtaac tgacaccttc taatttgttg gatttttcaa
     3481 attctctcat cacttagcca atccctcatt tcaaaaagtt agaactttcc aaattttttg
     3541 ttatttacta tttcaatatc atactccatc ctagaaaatt ttgccactta tttaggaaaa
     3601 acgtaagtat taaaaaaaca caaacaagcg ttgagaactt aattgtcatg atagtcattc
     3661 catagattcg ctgcccaatc cattgcaatt ccacaaatga ttcctagcat aaaaccaagc
     3721 aaaaaacgtc cgaatgcaag tccaatcaat acacctaaga tcattcccag tcctgaaaat
     3781 tctccgtcat atttctccat cttaataagt cctcccattt ttgagcttga caactatgta
     3841 aaacctcgct ataattagtg tgttattatt acgcagatgt gttggaattg gcagacaagc
     3901 atgattgagg gtcatgtgag cgttagctcg tgtgggttcg agtcccatca tctgcactac
     3961 aactcgtgaa gttaattttg cttcacgagt ttttttgttt gaacgtttct ttatgccatt
     4021 atatacatat cactgtatcc tttacaaaaa gaacgtacaa tcatgtaaac agcctgaggt
     4081 gagtatatga aggtcggaaa caagctactc gtagaaactt gcaagtgtta aaacaattaa
     4141 tcgaaaaacg agtaaaatcc ctcacctaat actaaagcga gggatttttt gtatattatc
     4201 tgtttatcac cgttattata aaggtacaaa taccaagcac ggttaatcca aattgcataa
     4261 attcattttt ttgtacactc atgcttttca ttagctcttt aactgccatt agtactaaga
     4321 tggcgataac aacataaagt atatagtacg tccgcattcc ggaagcccat actgtgggag
     4381 aaaatcccat aattaacctt gaacaaaatc ccattatcat caaaataact aaaaaaatag
     4441 aagttttctt attgtcaaaa cttaaataaa ttccaacaat taaacatagt aataatgcca
     4501 agactactaa agtagctatc caagtacctg gataaaaaag tgacagtttc gttcctaact
     4561 ctgtaaagag attattcagg ttactactac tccaaatcat tgccctttta tttccattga
     4621 cgtaagtaaa gctttgaccc attgtattac caaaataaat aatcaggttt aagaaaaacg
     4681 gtaatgcagt caatatacgc acataata
//
LOCUS       DAXWOO010000179         4462 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.183, whole genome shotgun sequence.
ACCESSION   DAXWOO010000179 DAXWOO010000000
VERSION     DAXWOO010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.183"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..253
                     /locus_tag="ACGE70_002537"
     CDS             <1..253
                     /locus_tag="ACGE70_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFF8694137.1"
                     /translation="NSLRYLEFREGAFDFQTSQQNPVGSGDATVAGLTAAIQQGLSDE
                     AVLKQGNVLGMLNAQEATTGYVQLANYQKLYDQILVEKV"
     gene            266..1252
                     /gene="lacD"
                     /locus_tag="ACGE70_002538"
     CDS             266..1252
                     /gene="lacD"
                     /locus_tag="ACGE70_002538"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFF8694138.1"
                     /translation="MNEEKRQYIKQLCNEDGVIGALAIDQRGALKKMITKFKGSEAEE
                     SEIVEFKEIVSSQLTKYASSILLDPEYGLPAADVRAKNAGLLLAYEKTGYDASTPGRL
                     PDLLSIWSVKRLKEAGADACKFLLYYDVDEAEEINNQKHAFIERIGSECQAENLPFFL
                     ELVSYDANISDATSKEYAIKKPHKVNDMMKEFSDPRYGVDVLKVEVPVNMNYVEGYGT
                     DEVVYTKAQAANFFKEQAEATNLPFIFLSAGVKAELFQETLRFAKNAGSTFNGVLCGR
                     ATWADGVESYIVDGKEAAMEWMNTQGRKNIEELNAVLKETATPVEVLTSSLS"
     gene            1708..2376
                     /locus_tag="ACGE70_002539"
     CDS             1708..2376
                     /locus_tag="ACGE70_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFF8694139.1"
                     /translation="MRVLVVEDNRELVLSVKKGLEQENISVDVAYDGESGEEKAFINS
                     YDGILLDLNLPKKDGLSVLQSLRAEEVESPVIIITARDEIEERAKGLDFGADDYLVKP
                     FELLELKARIRAVIRRYHGRALPQISIGALKIDPLGRRVSYQKTEVVLKPKEFDILLC
                     IAQRHPAVVSSEEISEHVYDEDFDPFSSVLRVHLARLKKKLAEAAEKEVLKTIRAKGY
                     QLCY"
     gene            2367..3500
                     /locus_tag="ACGE70_002540"
     CDS             2367..3500
                     /locus_tag="ACGE70_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFF8694140.1"
                     /translation="MLLNFKMTLTMLAFSLLVTGVILVSFFFLRNNLEGISIASTLGE
                     DVYFMEEGKTVQAETQMEASKSSNNQFMTISMIDKAYQDELFKYLPAMVLVICSAVML
                     LTFILWLVLRKIYSNQILAITRDLTFIDQVPKNTVKDEALNAAFNKLKDKFDGHLEDY
                     RRLNSYLTHEQKNAIAILRTNLEIERQHADQGNLMLLDRMTDSIDDILTLSNNSEDLS
                     TEVVDVSLICAEVCDAYKMNYPNLLFAFTEDQPTLIHGRDRWIYRAVSNLVDNAIKYG
                     EGKLVIVTISNQKGSVIIEVKDQGIGIPQEKQASIFSHQYRVHGLKQDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            complement(3795..4220)
                     /locus_tag="ACGE70_002541"
     CDS             complement(3795..4220)
                     /locus_tag="ACGE70_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFF8694141.1"
                     /translation="MHLHRISALAVQIGRYHHSETRAVMEGLEEAIQTTNDCPYRRTF
                     HSDQGWSYQMKAYRNKLKEYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCF
                     EELKSAIDSYIYYYNNERIKQKLNWQSPVQFRKTTVTVV"
BASE COUNT         1419 a          761 c          977 g         1305 t
ORIGIN      
        1 taacagccta agatatttgg agttcagaga gggcgcgttt gattttcaaa cttcgcaaca
       61 gaacccagtt ggctctggtg atgcgaccgt tgccggattg accgctgcga ttcaacaagg
      121 tctttcggat gaagccgtgt taaaacaggg taatgtttta ggaatgctga atgctcagga
      181 agcgacgact ggctatgtcc aattagccaa ttatcaaaaa ctatatgatc aaatcttagt
      241 ggagaaagtg taggaggata cggaaatgaa cgaagaaaaa cgtcaatata tcaagcaact
      301 atgcaatgaa gatggagtga ttggggcctt agcaattgat caacgcgggg ctttgaagaa
      361 gatgatcaca aaattcaagg gcagcgaagc agaagaatca gaaatcgttg aatttaaaga
      421 aattgtttct tcacaattaa cgaaatatgc ctcatcgatt ttattagatc ccgaatatgg
      481 cttgccagca gctgacgtta gagcaaaaaa tgccggcttg ctgttagctt atgaaaaaac
      541 tggttacgat gcgtcaactc ccggccgttt accagattta ttatcgatct ggtcagttaa
      601 acgtttaaaa gaagcaggcg ccgatgcttg taagttccta ctttactatg atgtcgatga
      661 agcagaagaa atcaataatc aaaaacatgc ctttattgaa cgaatcggct cagaatgtca
      721 ggctgaaaac ttacccttct tcttggagct tgtttcttat gacgcgaaca tctctgatgc
      781 gacttcgaaa gaatacgcca ttaagaaacc tcataaagtc aacgatatga tgaaggaatt
      841 ttctgatcca cgttatggcg tagatgtgtt aaaggtggaa gtacccgtca atatgaatta
      901 tgtggaaggc tatgggacag acgaagtcgt gtacacaaag gcacaagcgg ctaacttctt
      961 caaggaacag gctgaagcca ctaacctgcc atttattttc ctaagcgccg gtgtaaaagc
     1021 agagttgttc caagaaacac tgcgcttcgc gaaaaatgcc ggctcaacct tcaatggtgt
     1081 actttgcggc cgtgcgactt gggcagatgg tgtagaaagc tatatcgttg atggaaaaga
     1141 agcagcgatg gagtggatga atacgcaagg gcgtaaaaat attgaagaat taaatgccgt
     1201 attgaaagaa actgctacac cggtggaagt gttaacaagc tcactatcat aagaaatcag
     1261 actgtgaagc aaaattgctt gcagtctttt tttatacata aagcgtaaaa tcatgctgca
     1321 ttattagggg aacgaattct aaaatagtaa gtgagttaaa acgaaggggt ttttctttag
     1381 agagattttc ttacatgtgg tacttaatga cttgatggca attttcagta ttctaacgag
     1441 gcttattggt cataaaccaa cgaactttga aaagtatata caagaaaacc aagcaaatag
     1501 aaagtagtga gactggagga aaggttccag tctttttcta ttctaggtat tttcaaacag
     1561 taaaatcatt cttaattatg tgttttttaa cagaatgtat gaatttcttc aaatcttcac
     1621 aaaaagaacg tggactttga agaagagcgt gtactatgct aaaaagcata agtgtaatta
     1681 ctaaatccgt atgaggagtg aatatacatg cgtgtactgg tggttgagga caatcgtgag
     1741 ctggttttat ccgtaaaaaa gggcctagag caggagaata tttctgtaga tgtagcttat
     1801 gatggcgagt caggagaaga gaaggccttc atcaattcat acgatgggat actactagat
     1861 ttaaatctgc ctaaaaaaga tggattatct gttttacaaa gtctccgtgc tgaagaagtc
     1921 gaaagtccag ttatcattat tactgcacgt gatgagattg aagaacgagc aaaaggcttg
     1981 gattttggag cagatgatta tttagtcaaa ccctttgagt tactagaatt aaaagcacga
     2041 atacgcgcgg ttattcgtcg ttatcatgga cgtgcgctgc cgcagatttc aattggtgct
     2101 ttgaagattg atccattagg tcgaagggtc agctatcaaa agacagaggt ggtcctgaaa
     2161 cccaaggaat ttgatatctt actgtgcatc gctcagcgtc atccagcggt tgtttcgtct
     2221 gaggaaattt ctgaacatgt ctacgatgaa gattttgatc ctttttcgtc ggttctgcga
     2281 gtgcatctgg ctcgtctaaa aaagaaactg gctgaagcag ctgaaaaaga ggtcttaaaa
     2341 accattcgag caaaggggta ccagctatgc tattaaattt taaaatgacg ttaacgatgc
     2401 tggctttttc actgttggtg acaggcgtga ttttagtgag tttcttcttt ctgagaaata
     2461 atttggaggg aatcagtatc gcatctacct taggtgaaga tgtttatttt atggaagagg
     2521 gtaagacggt tcaagcagag acgcagatgg aagcttcgaa atcttccaac aatcaattta
     2581 tgactatttc gatgatagat aaagcttacc aggacgaact atttaaatat ttaccagcga
     2641 tggtgctggt tatttgttct gccgtcatgt tattaacgtt tattctttgg ttggttttac
     2701 gcaaaattta cagtaatcaa atcttagcga ttacgcgtga tttaacgttt attgatcaag
     2761 ttcctaaaaa tactgtgaag gacgaggcat taaatgcggc ttttaataag ctaaaggata
     2821 agttcgatgg tcatttggag gattatcgac gattaaatag ttatctgacc cacgagcaga
     2881 aaaatgccat cgctattttg cgtacgaatc tggaaataga acggcaacat gccgatcaag
     2941 gaaatttaat gcttttagat cggatgaccg acagtattga cgatattttg acattatcca
     3001 ataactcaga ggatcttagc acggaagtcg tagacgtttc actgatctgt gcggaagttt
     3061 gtgatgccta caaaatgaat tatccgaatc ttttgtttgc gtttactgaa gatcaaccaa
     3121 ccctgattca tggacgagat cgttggattt atcgggcggt ttcgaattta gttgacaatg
     3181 caatcaaata tggtgaagga aaactggtaa ttgtcaccat ttcaaaccaa aagggtagcg
     3241 tgattattga agtaaaagat cagggaattg gcattcctca ggagaaacag gcatcaattt
     3301 tttctcatca gtaccgagtg catgggctca agcaggatgg cttacctaaa acaatggact
     3361 ttagggacac ggatgaaacc tttattcaat ccgttgcctc gaaaagaaac catattccaa
     3421 gaaaatcatt aaattatcga acaccattgg aagtattttt gagctgcata gataaagaca
     3481 tattgtctag cttaatttga caaatcagat gtctataaaa ttcgttagaa aatacaattt
     3541 atagatatcc aattgatttg taaacaaaaa ggagttgtca actaagccac tcctttttgt
     3601 tgtaattttt cccaataaga aagtctacta ctttaggaag ataatagtaa gatagaaatg
     3661 ttgcccccaa aattattatc cagctggata taattaatca aataaaaagg atcaaaaatg
     3721 tagtgacccc caaaagttag acttttttgg agtggagaaa tccactcttt ttttcatgct
     3781 ctaaaatgat atttttacac aaccgtaacg gttgttttac gaaattggac gggactttgc
     3841 cagtttaatt tctgttttat ccgttcatta ttgtagtaat agatatagct atcgatggct
     3901 gattttaatt cctcgaaaca gttataaact ttcccgtgaa aaatctcttg ttttaataag
     3961 ccaaaaaaat tctccataag tgaattatct aagcagttcc ctttccgaga catgctttga
     4021 aagattttgt actccttcaa tttgtttcta taagctttca tttgatacga ccaaccttga
     4081 tctgagtgaa acgtacgacg atagggacaa tcgttagtgg tctggatagc ttcctccaaa
     4141 ccttccatta ccgcacgtgt ttctgaatgg tggtagcgac cgatttgaac ggctagggcg
     4201 gaaatacggt gcaaatgcac ggtggtttca tgcaatgctt acagggcgta ttttgtaagg
     4261 gtatgtaacg gagataatcc cgacggtaat ggatgtgaga ggccaagccg acgctgacgg
     4321 tagggcaaac aaacatagtt gcactctagc gatcgttatt tatttttaac ggtcaaacta
     4381 gggtacaact atgcttaaaa cacttcgcct caacttctgg tagcaaaagt gattttgaaa
     4441 gtcaaaatat tttttatgag tt
//
LOCUS       DAXWOO010000180         4418 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.184, whole genome shotgun sequence.
ACCESSION   DAXWOO010000180 DAXWOO010000000
VERSION     DAXWOO010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.184"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            59..913
                     /locus_tag="ACGE70_002542"
     CDS             59..913
                     /locus_tag="ACGE70_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFF8694142.1"
                     /translation="MENSIYLVTEPNTQLKDCYFNFCGYSKTEPRHSFGPAIREQYLI
                     HIVLEGEGYYSINDQKYFLKKGQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYY
                     LKEIGVGKNHLTFKVENLNSFKGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSAS
                     YLNEGIITKKMNPFVRQTIEIIRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISI
                     KEYISEIKISIANDLLSSTEYSIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRL
                     GLGIINGE"
     gene            1010..3259
                     /locus_tag="ACGE70_002543"
     CDS             1010..3259
                     /locus_tag="ACGE70_002543"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFF8694143.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKIPEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3262..>4418
                     /locus_tag="ACGE70_002544"
     CDS             3262..>4418
                     /locus_tag="ACGE70_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFF8694144.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTRV"
BASE COUNT         1556 a          626 c          807 g         1429 t
ORIGIN      
        1 gtaataatat aaacataata aaacaaaaat tatccaaatc taacatagga gggtggatat
       61 ggaaaattct atatatttag taacagaacc taacactcag ctgaaagatt gttactttaa
      121 tttttgtggg tattcaaaaa cagaacctag acatagtttt ggaccagcta ttcgagaaca
      181 atacttaata cacatagtgc tagaaggtga aggctattat tcaattaatg atcaaaaata
      241 ttttctgaag aaaggccaag gatttgttat cccaccagga gtatctactt tttatcaagc
      301 agatgaaaaa aatccgtggt catatgtatg gttgggttta agtggcaatc ttgtagaata
      361 ctatctaaaa gaaattggtg ttggaaagaa tcatcttacc tttaaagtcg aaaatttaaa
      421 ttcatttaaa ggattaattt ttgaaacttt atcgtatgat caggacaatc tagtcagtga
      481 attgtccaga caaaaacaag tttataaatt tttagaactc ctcttaaatt cagctagtta
      541 tctgaacgaa gggataatta ctaaaaagat gaatcctttt gttcgtcaaa caattgaaat
      601 aatacgtgag aatgcgtatc aaaaattatc tgttaaagat attgctgtca gtctatcgat
      661 acacgaaaat tatttatcac gattgtttaa gaatgatatg ggaatatcaa taaaagagta
      721 tattagtgaa atcaaaattt ctattgcaaa cgatctgtta tcatcaactg aatattcaat
      781 ccagcaaatc agtgacaaga taggattctc aacagtacaa tctttttcta aagcctttaa
      841 gaaagcaaat ggtgtttcac cgggagtata tcgaaaacgt agattaggtc taggaatcat
      901 taatggtgaa taaactaaac atttgatttt gataaatatt cgttacttta aacgcttact
      961 atttatgtga gcgtttttta taatgcaata aagaaacgga ggagagctta tgtataagga
     1021 taatgaaatg aatattattt atcgatcaga aaaccaacaa tttcatttgt ttaatcagga
     1081 gataagttac atttttaaga taccggaaga tggtaaatta ttgcatttat attatggtga
     1141 gaacttacca gataatgact atagtcatct aattgagatg catcatcggc ctatgacaac
     1201 ctatagaaat gaaaacgatt tgctatattc gttagagcac ttaaaacaag aatttcctga
     1261 atatggtaca acagatttta gacatcctgc tatttcatta ctacagaaaa atggctctcg
     1321 cttatcagat tttgtctatc aagggtacga agtggaagaa ggaaaaccta aactagaagg
     1381 tttacctgct acctatgttg ataataatga tgaaagcaaa acattgaaag tttatatttt
     1441 agattcagtt acaaatgttc aagtagaatt attatataca atttatagag attatcctgt
     1501 tattactcgt agcgcgcgag ttatgaataa aggaaaagat attcaaataa tcgagaatat
     1561 tagcagttta tcattggatt tacccgatgc aaattatgaa tggcaacaat tatctggtgc
     1621 ttggggtaga gaaagaacta ttaagtcgcg tactttacaa caaggaattc aatcaatcga
     1681 atctactcga ggtatttcaa gccatcagca taacccattt gttactttaa aaagaaaaaa
     1741 tactgatgaa aatcaaggag aagctattgg tgcagcacta gtttactcag gaaacttctt
     1801 aattcaagca gaagtagata catgggatgt tactagacta caagttggca tcaatccatt
     1861 tggatttaat tggaaactca atccaggaga aatatttaca gcaccagaag cgattctggt
     1921 ttattcaagt aagggtttaa atgccatgag tcaaactttt catcaactat ttagaaaacg
     1981 cttagctcgt ggtaagtggc gagaaaaaga ccgtcctgtc ttaattaata attgggaagc
     2041 aacatatttt gattttgacg aagaaaagtt ggtaaatatt gctaaaaaag catttgatgt
     2101 tggtattgaa ttatttgtat tagatgatgg ctggtttgga gaaagagaaa acgattgtgc
     2161 aggattagga gattggcatg taaatcctaa aagactacct agaggaatcg gcagcctttc
     2221 agaaaaaatt cgtagttttg gtatgaaatt tggtttatgg tttgaacctg aaatggtgaa
     2281 taaggatagt gagttatatc gcacacatcc gaattggatt atttctacac ctaatcgaaa
     2341 acctaatcat ggtaggaacc aatatgtatt aaattttggg ttagatgagg ttgttgaaaa
     2401 tattttcgat caaatgtgta aaatcattga tgaatcaaac ttagattaca ttaagtggga
     2461 tatgaatcgt ccattgactg atgtatttga cagtcacttg tcagcagatc aacaaggaga
     2521 agtattccac agatatgtac taggcgtata tcgcttatat gaaaaactta taacaaaata
     2581 tcctaacata ctatttgagt cttgttcatc aggtggtggg cgctttgatc ctggaatgct
     2641 tcactatgca cctcaagctt gggctagtga tgattcagat gcaattgaaa ggttaaaaat
     2701 tcaatatgga actagtatgc tgtatcctct ttcgtctatt ggtgctcatg tatcaattgt
     2761 tccaaatcat caaacaaacc gtataacacc tataaaaact agaggaaatg tagctttttt
     2821 tggtgcattt ggatatgagc ttgacttaaa tgaattaagt gaaggcgatt tgaggattgt
     2881 aaaagaacaa attgcctttt ataaaaaata tagaaccata attcataatg gtacttttta
     2941 tagactatta tcaccattcg atgatgataa tcaaaccgcg tggatggttg ttagtcatga
     3001 taagaaaacg gctattgttg ctcattataa aacattaaat gaggtaaatg ctccttaccg
     3061 ccgactgaaa ttaaaagggt tagataatga cggactttat aatgtaggac aaaatagttt
     3121 gcgatctgga atggaattga tgagagcagg actagtctgt tcagatgcat ctagtggaca
     3181 aataaatgat cagaatcgac gcccagaaac atttgatttt gattccaaga tttgggttat
     3241 aacacaggag gaaaagtaga gatgaataac caaaaaagct tacttcaaaa aggagcaatc
     3301 ttaagtattt cactaatgtt aacaagctct caagcaatta atggtgttat tccacaaatg
     3361 aaacagtctt tggggatatc acaaagtcaa agtgaacttt taggaactgc accaagtata
     3421 actgttattt tgtttatctt attatctagt tactttgcca aaagaatagg aatgaaaaag
     3481 acaataattt taggtttatt attagctggt ataggaggaa ttattccagt atttacagct
     3541 accttccaaa ccgttcttat tagtcgagta atattaggtg ccggtttagg agtatataat
     3601 tcattggctg tgacatatat cagctcttta tactctggca atactagagc aacgctatta
     3661 ggtattagaa attcaatgga agctttaggt cagacaatcc ttatttttct tgctggagtt
     3721 ttagtgaaca ttaattggac ggcttcgttt ttagtctatg caattgcgtt tcctgtagct
     3781 ctcttattcg cgctaaaagt tcctgaaatt agagatgaaa atagtgacat acctgaaaat
     3841 catgtaaaag aaaagatgaa tccaatggtt tttgccctag ttctatttgc aatcttactc
     3901 gtaatgaata gtattgctat ttctgtacga tttgcatcaa ttgcaactga aattaaaggg
     3961 gtaaacttta atgctagtaa ctatttagct ctaatgccta tcttaggaat actagcagga
     4021 tttatgtttg gaccaataaa taagtggata ggaaaaggaa ccctatactt gggaattata
     4081 ttctttataa tttcaaattt gttaattgct gtgtcaaacg gtaatatgac gttcctgcta
     4141 acgggtttat ttttatcaag tataacagga tcatggtgct tcccatttat ctttagtaac
     4201 ttagataaag tcactacaaa gaatacaatt aattttgcta cttcattgat ttttattggc
     4261 tgtaatattg gtaattttat agctcctatt gctatgcaat tagcacaatt tctaacaaga
     4321 agtacaaatt tgactgctcc attttttgta ttcgcaggaa tatttattgt gatcctattt
     4381 gtaacatttt ttgcgacaaa taaaaaaaat actagagt
//
LOCUS       DAXWOO010000181         4408 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.185, whole genome shotgun sequence.
ACCESSION   DAXWOO010000181 DAXWOO010000000
VERSION     DAXWOO010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.185"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            164..1465
                     /locus_tag="ACGE70_002545"
     CDS             164..1465
                     /locus_tag="ACGE70_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFF8694145.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1492..1752
                     /locus_tag="ACGE70_002546"
     CDS             1492..1752
                     /locus_tag="ACGE70_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFF8694146.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2751..3986)
                     /locus_tag="ACGE70_002547"
     CDS             complement(2751..3986)
                     /locus_tag="ACGE70_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFF8694147.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1298 a          696 c          894 g         1520 t
ORIGIN      
        1 tcctatgatt tagaaaactt attttcagga ttcttaaaaa taaatttgat tatttaagag
       61 aatatattga aataaaattt cttaattata taattagtta tgaacattct ttgcgttgtc
      121 atgcaatgaa tggttcatat tttattgaaa aggagtggtt ttagtggaaa agctgatgaa
      181 atgggtcgaa gagaaacttg tcccaccaat ggcaaaaatt ggtacacagc gtcatttatt
      241 agcaattcgt aatggggttg tttcaacatt gtcattgatt ttgattggga cattttttat
      301 ggtctttatc aatttaccgt ttcctggatg gaatgaattt attgcccctt attcggcaac
      361 gattgtttta ccctttagga tgacgatggg attgatggct atttatgcaa cttttgttat
      421 gggttcggat ctagctaaat catatggatt agatagtgtg acaggtggta tattatcttt
      481 aggaacgttc tttatgttac aagttcctgt aaacgtttta actcctgaag aggcacctct
      541 tggctgggtc ttacctatga gttctttggg agcatctggg atgtttgcgg ggatcttatc
      601 tatgattttt gcagtggaag tatatcgttt ctttaaaaat cgaaatatta ctatcaaaat
      661 gccagaacaa gtgccacctg ctgttgcccg tagttttgag gcattgattc caggtgcagt
      721 cattttaaca attacttggc tgattcgaag cgtcgcgggc tttgatgtaa acgctgcctt
      781 attatcgtta ttcgagcctt taaccaatat tttagggaat aatttgcttg gtgttttact
      841 accaatgttt ttgatccatt tgttgtggtc atttggtatc catggcatga gtattgtagg
      901 ggctgttgtt cgtccaatgt ggctgatcat gttggatgag aatgcaaagg cattggcaga
      961 tggtacagct gccacccaat taccctatat cgctcctgaa cagttttatc aatggacggt
     1021 aacaattggt ggtgcaggag caacgatcgt ggtatctgtt ttgtttatat tcttctgtaa
     1081 atcaaaattc ttgaaagagg ttggtcgttt ttctattatt cctggtatct tcaatatcaa
     1141 tgagcccata atttttggag ctcctatgat gttgaatcct tacatgttca tcccgttcaa
     1201 cttagttcct ttagttttga ctattatcag ttattttgca gtcaaattag aaatggtcaa
     1261 tagatttact gttttgccaa catggacatt accagctcca atcggcggtt atctttcagc
     1321 aggaaatgac tggcgtgttg ttatcttgat catgatcaac acattgatag catttattat
     1381 ctattatcca tttgtaaaag cttatgataa aaagatgctg tcagatgaac aaagtcgtgg
     1441 agaaaacgaa gtccagactg tctaagtttg gtttttagaa ggtgggaaac aatgtattgg
     1501 ctgattttat tttttgtttt tatcttttta ttaacggcta gtcaccttat attgaatatg
     1561 ctagctacat atcatattca aatcaatcga tggatttggg cactggcatc atttttgatc
     1621 gtgattcttc caaagatcat tgtaccgcat atgaatgttt tgttttcatg ggggacatat
     1681 gtactttgtg gaatttttgc tatcaatttt atgatcgagc agcatagatg gtttgtcaca
     1741 tcgaagcttt aaatgttcta aaagtggttg taacagaagt ggttagcttc aagaaataag
     1801 aagaaattta cgaaaattgc ttcttaaatt tttgtgaaat gttggcttat ttttcgtttg
     1861 gttgaactta ttaccgcaga tgcaagcttt tgaccaccgt ttattcggaa tatgggactg
     1921 tgacaaggct tttgtcacag ccctttttta cataaagatt attattgttt taactgtttt
     1981 ggcagaaaga aggaggaaaa aatggaaatt ttatcagcaa tggatttaga attagtagat
     2041 ctactaccaa agtgatcaag tcaaatatag atttgggagc ttacaaccat atagtagggg
     2101 ctgcagtatg gtgtatgaat ggtaatatgt atgatgtgag tgtttattca aatcatgaaa
     2161 cgtgtataga aataattgtg ctgggaatag caatcagtca tggtgaacgt gacttttagt
     2221 gtatcgttgc tgacgacata gaacattctg acatgatcta ctcaatatgt ggggattgct
     2281 ggcagtttat ataggagtat acatcggatt gcgagatcat tgtctcaaca gaagagggag
     2341 gttgattatc aaagataaag tggtaaatcc tacatttgat ttagtaattg ttgaggggac
     2401 tagcggcaca aatctctaca aagcgatgct tctagatcta tatgacacat tttaagaaaa
     2461 tgtatttgcc taattattat ggtctttctt ttgaagaaac gttgagatga tccaaccaga
     2521 gaaaggtgtc aaagaatttg actaagaata aatggcagac gctacctatg ctcatttgga
     2581 tagcgtcaga tcttttattt taaggatgat tttgtgtttt ttagacaccg taatttaagt
     2641 aactctaact tttcaatcca ctatttactt tttttgtatt ttcactgtct gggtctcgaa
     2701 tagcgaaatt actgatgagt ctctattaaa aaagcttttt ctacttgaaa ctatattggg
     2761 tggttgctca ggaaggtttc gatttcttga attgcttgtt gtggaccatt tattggaaaa
     2821 acggcttgca gcgtgcgtgc ctgctgacga atcttgggtg aagaaacttc gcgcagagca
     2881 atctgcagtt ttctttcagt cactggtttt gtgagggcga ttgcgtttcc gtattttaca
     2941 caatagtcaa tattacagct ttgctcaaat tgcatcccca caccgataaa cggtttgccg
     3001 gattcacagg cagtttggac ggtcccttcc ccgccgtgga taaaagaaaa atcaatgaat
     3061 tcatgcaaaa gatgactaga cagatattct gttattaaaa gatcttctga gtcagattga
     3121 atgtcatcaa gcagatagtc aataggtgca ataaccgcta gatccggttg tttttgtaag
     3181 aagaacaaaa ttttgcgtaa catacgcgga ttgcctgaac tacccattgc gaaatagata
     3241 atacgttttc tttcttgttg ctttcgctgt ataaaagtta gcacttcttc aggcaaggaa
     3301 gtactgagct tagcaaaaat tggaccaacg attcgataat tttccggcag ctgttcttct
     3361 tccaaaaata gtttaggcgt tgtgatgaga tttagatcag catccatcat ttgtaaactt
     3421 gtttttggta aaggaagcat ttctttttgt gcagcgtgtt tgaaggcatt ggggatccat
     3481 cgggtattta ctatacgctt ttttgtccag tgccaaaggg gattgcttaa agggccgaag
     3541 aaacgtaact cgtggggcat cagcgcatgc tctgattgaa aaaaaggttc actcaaagca
     3601 tagggtttaa catatactaa aggaatcttt ttattgcggg ccgataaaaa aattgtcaca
     3661 ttcgaacctg tcacaatcag tcgaggctca aacttagcaa tcagactctg ttcactagtg
     3721 atacgagagg caaccatttc tttggtaaaa gggtttttaa gtgtacgcaa ctggtcaaat
     3781 tttatgattt gttcttcttg ttttttcgta aggatcggcg ctagtaaatg atactcaaag
     3841 ccactttctg tgatcaaagg agcaaaagtt ttagaaaagc caaagaatac acagtaataa
     3901 cggtctttca tagctttggc aacctcaatc attcgtgttg tctcagctaa attgaatact
     3961 gcaggagcaa aaagaattgt cttcatgaaa tcctccttat tggaatgagt ctatcccttc
     4021 gtttcttact agtatactaa aaaacacagc gttcgagtag cctatgtata ttttgaatat
     4081 taaactgtct ctcgctgttt tcacaacttc taaatttaaa ttttgtaatc aagttgaaac
     4141 tccaattttc aaaaaaacac gcaacaattt tttgatcgat ttatatgttg tgccagataa
     4201 atacgagaag ttgtgtcatt aaaaataaac atgagaacat aaaaaaataa aataattata
     4261 ttaattgata ggtgtgctgt cgtcattata cacacgtaag aaagaaatag ttatgtttaa
     4321 aaatattctt agactttctc gccatgacaa ttttcactat gtgttataac tgtaaacgat
     4381 aattattcag ataacacaaa aagaaatc
//
LOCUS       DAXWOO010000182         4365 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.186, whole genome shotgun sequence.
ACCESSION   DAXWOO010000182 DAXWOO010000000
VERSION     DAXWOO010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.186"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            212..451
                     /locus_tag="ACGE70_002548"
     CDS             212..451
                     /locus_tag="ACGE70_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694148.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            508..636
                     /locus_tag="ACGE70_002549"
     CDS             508..636
                     /locus_tag="ACGE70_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694149.1"
                     /translation="MQPLQMLYDNKPELNDPDISFAFFEMIGSDDLFKTTSLSIRK"
     gene            complement(813..1073)
                     /locus_tag="ACGE70_002550"
     CDS             complement(813..1073)
                     /locus_tag="ACGE70_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694150.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVIGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(1258..1755)
                     /locus_tag="ACGE70_002551"
     CDS             complement(1258..1755)
                     /locus_tag="ACGE70_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFF8694151.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(1885..2595)
                     /gene="budA"
                     /locus_tag="ACGE70_002552"
     CDS             complement(1885..2595)
                     /gene="budA"
                     /locus_tag="ACGE70_002552"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFF8694152.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(2608..4272)
                     /gene="alsS"
                     /locus_tag="ACGE70_002553"
     CDS             complement(2608..4272)
                     /gene="alsS"
                     /locus_tag="ACGE70_002553"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFF8694153.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
BASE COUNT         1267 a          914 c          691 g         1493 t
ORIGIN      
        1 tataaatttt ttcataaaaa aagcgtttag aggcataatc gttaatacag atggtaacca
       61 atgtatctgg tttgaactgg gatatcaccc tctccatata tgtattacta agactaatag
      121 atactataaa agacttgata gttttgcatt gattttgcct aatactgagc ataggtgtat
      181 gctaaacgat actataagga gtgtgtacat aatgaaaaga attctgttta tcagtttatc
      241 aaatttattg ttacttactg cttgtgggac ttctgcatca aagaacatac cttcagatac
      301 gcaaatttcg aatagtatcg attcaacaat ttctacaaat gagaaaaatt ctccggtaga
      361 aacttccgat ataaaagttt ctacctctgc aaaaaacagg gtaaagaaac tgtcagcacg
      421 tctcctttat ttcaaacaaa ctctgaatta gcttcttcat cttcttcagc aaataatgat
      481 gatagtttcg cgtttactcc taactaaatg caacctttac aaatgcttta cgataacaag
      541 cctgagctaa atgatcctga tatcagtttt gcattttttg aaatgattgg atcggatgat
      601 ctttttaaaa ctactagttt gtctatccga aaataggaca gttctggcag tataggtttt
      661 tatcgagttt ctccaccagg cctcgttaca gagacagatt ctctaggcga accttttgaa
      721 taaatcaaaa atcatgaaca atcactgcca actaaataaa gtgccttcaa aagcttggtc
      781 ttctcgcttt tgaaggcact ttatttaaat ctttatgaaa ctaaagtatg ttcatatttg
      841 ttgctatagt tatatagata ttctgttgtt tccaactcat aaacgtgagt atattttgcc
      901 actacttttc cgcatttttc taatatttca ttgcgatcga cgtcatgata ttcgtctacg
      961 cagatcacaa cacaattttc taatttactg attacttctc caatcacttt ttgtttcaag
     1021 ccaataggtt ggcaaatata tttttttcct aaaatgatag tttcgttatt catatttcat
     1081 ccatccttaa tgttttttca ttatttaaaa tgataataac attaaaatat gattatgaaa
     1141 atcaataaca cttgtgattt tataaaaaaa taaaaaaacc gaaaaacaga aatgcccact
     1201 gtctttccgg ctctttttat tcttgatttc tcttcttttt tgtttcaata gacgatttta
     1261 gttattaata aatgaaaaag catttatatt aaaatttgct aataaaagat ctacatattt
     1321 ttctagaaat gcttgatcga tttcatcata ccctttcgtt actccgcaat cgatatctaa
     1381 aacaccgatt aaattacctt cttttaccat tggcactacg atttctgatt gtgcggctga
     1441 atcgcaggaa atgtaatttg catgggtttt gacatcatca acgatcaatg tttcttgttt
     1501 ttcagctgat tctccacaaa ctccttttcc catcttgata cgtgtgcaag aaactcgccc
     1561 ttggaacggt cctaaaatca attcattacc atcgaataaa taatatcctg caaagacagt
     1621 ctcaggcaaa gtctcattca acaatgcaga tgaatttgct aaatttgcta accagttatc
     1681 ttctgcttct aataatcctt gttgctgtaa taataaaatt tcgtatgcat cttttttctg
     1741 tttttctgtc cacatgataa aaaccgctcc tttttgatct atggagaaga ttatagctca
     1801 gtttttttgt aaacgctagg ttttctcctc aaaaaaacga gatacgtcag gttaatcacc
     1861 ttgaacgcat ctcgctgtct ttttctattc tgcttgttgg atttctgctg ctaaatcagc
     1921 ataatcaatc gtttcgtgaa caaaagtcgg atcatcaact ggaaaatgct gtacaaggga
     1981 cgagatgcgg ctaatctcaa cttttccttc agccacttca aaatccatta catgtccgcc
     2041 aaatgtatgt gtatcatcga taaaatgcag gtgaaatccc gctgcagcaa ctccttggaa
     2101 taattcaggc gtaaagaacc cgactaatgt tccttggaca ttgctttgtt ggaattctgg
     2161 ttgagactct gaaatttttg ctagtcttct gtaaggtttt tcttgcttcg gcatgatacg
     2221 gatgtgcata tttttgaata atccagttat cttcactgcc aaaaataggt tttccccggc
     2281 ttctcttact aatatgtctt ttttcaattg ttctccatct gcagtttttg agacagaaaa
     2341 actagtatct ggcgtaaaat ctgtaattgc tgcatagggg gtaagttcac ttccatctaa
     2401 ttgggcaact gagccatctg atcttccttg ataagctacg ccatctaaaa agatgacttc
     2461 accatcaagc ccatgcaatg ttcccaatcc cagagttcct ttttctaata tatgagcaat
     2521 cgtttctgtt ccatccatca agccggccat caaagctcct aatgtcccgt gctggtataa
     2581 gattttttca gtcatgtcga cacttcctta atagaattga tctggtaaga ttgtttctcc
     2641 caatttctca ttatcacggt aatcaatcgg aatatcaata attacgggtc cttctgtcgc
     2701 aaatccttct tctaaagctt tttctaattc ctctacactt gtagctcgga ttcctttagc
     2761 gccaaaagct tctgcatatt tcacaaaatc aactggacca aagtctacac ctgacgcccg
     2821 ctgatatttc attttttctt ggaactctac catattgtag tgtccatcat tccaaatcaa
     2881 atgaacgata ttcaattttt tacgtaccgc cgtttctaaa tcctgggcag agaataaaaa
     2941 tcccccatcg ccggaaactg atacgatctg tgtatttgga cgcaccaatg cagcagaaat
     3001 cgcccatggc aatgcaacac caagcgtttg cataccatta ctgaataaca aatgacgagg
     3061 ttcataactt ctaaagtggc gtgccatcca aatataatga ctacctacat ctacggtaac
     3121 agtcatatca tctgttactt ttgactgaag cgtgttaata acttctaaag gatgcaagat
     3181 acctgcttct ccttttgatt gaacaatgtc ccgctccgtt aattttgctt gaagagatgc
     3241 taaatattct tttgcatctt ctgacacttg ttgaggctct aatgaacctg tcaataaatc
     3301 aagagtggct gagatatcac cgatcaattc tctttcaggc tgcataaaag gatcgatttc
     3361 agctggcgct tcatcgatca caatgatccg cgcatctttt tccgcattcc agtttcttgc
     3421 ttcatattcg attggatcat agccaattgc aataactaga tcacttcgtt tcaaaagcat
     3481 atctcctggt tggtttctaa ataaacccac acgaccaaag aaatgcgctt ctaattcacg
     3541 tgagatcact ccagctgctt ggaatgtctc tacaacaggc aatgctgtct tttcaactaa
     3601 ttgcctgatg gctaacgttt ctttttcact tgatcctcgc atacctacta atagtaccgg
     3661 tagttttgct tctctaatgc gttcagctag gtaacggata tcatctgcag aagcagaacc
     3721 taatttagga tcgctcaatg gcttgatgac attaccttgg acaggtgcat ccactacatc
     3781 ttgcgggata ctgataaagc tcgcgccttt ttttgaactc ttagccatac gataagcatt
     3841 ggcaatcact tctgaaattg tttctggatc ttggatttct gcactgtatt ttgtgattgg
     3901 ctggaacagt gctgcattat ccatgctttg atgggtcaac ttcaatagat cgcttcgttt
     3961 tacttgtccg gcaattgcta aaactgggtc accttctgct gtcgctgtta ccaaacctgt
     4021 agctaagtta ctagcaccag gaccacttgt tgcgataact actcctggct ctcctgtaat
     4081 acgtccgatt gcttgtgcca taaatgcagc attttgttcg tgacgagtca cgattaattc
     4141 aggaccttga tcctctaatt cattaaatac accatcgatt tttgcacctg gtataccgaa
     4201 tacataatca acatgatgat taatcaaact ttctactaca gatttcgaac cttgtttcat
     4261 tttttcagcc attgaacatc gtccttttta tgctttattt ttaattatta gttagaaata
     4321 atcacaaata agtgatacag tttctaaacc tgtttcattc aaaag
//
LOCUS       DAXWOO010000183         4351 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.187, whole genome shotgun sequence.
ACCESSION   DAXWOO010000183 DAXWOO010000000
VERSION     DAXWOO010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.187"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            297..566
                     /gene="rpsN"
                     /locus_tag="ACGE70_002554"
     CDS             297..566
                     /gene="rpsN"
                     /locus_tag="ACGE70_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFF8694154.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            581..760
                     /gene="rpmF"
                     /locus_tag="ACGE70_002555"
     CDS             581..760
                     /gene="rpmF"
                     /locus_tag="ACGE70_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFF8694155.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            792..1169
                     /locus_tag="ACGE70_002556"
     CDS             792..1169
                     /locus_tag="ACGE70_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694156.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1190..1339
                     /gene="rpmG"
                     /locus_tag="ACGE70_002557"
     CDS             1190..1339
                     /gene="rpmG"
                     /locus_tag="ACGE70_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFF8694157.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1363..2346
                     /locus_tag="ACGE70_002558"
     CDS             1363..2346
                     /locus_tag="ACGE70_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFF8694158.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHREERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKHYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQGVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2376..2498
                     /locus_tag="ACGE70_002559"
     CDS             2376..2498
                     /locus_tag="ACGE70_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFF8694159.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2516..4039
                     /locus_tag="ACGE70_002560"
     CDS             2516..4039
                     /locus_tag="ACGE70_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFF8694160.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLIQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKLNKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNEMHNWPTFYPASLSEHEIAQEMMAH"
     gene            4063..4302
                     /locus_tag="ACGE70_002561"
     CDS             4063..4302
                     /locus_tag="ACGE70_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694161.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSV
                     DTVHRALKKDPDCCFIKVNTGRSRRLAKRYNILIN"
BASE COUNT         1619 a          628 c          870 g         1234 t
ORIGIN      
        1 ctttcatatt gccgttttaa gtagaggaaa agctacttaa aacggtattt gtgtttaatt
       61 ggactaataa aagtgaattc ttatttttca gagcttaaac tgcatattct tttaaatgat
      121 ttgattatct ctatgtaccg ataaattcgt tttcaaatag ttatctattc atatgagttt
      181 tatttcatgt tatttattgt tgacaagagc aactcttgtt ggtaaagtat cagtgtcaaa
      241 acgtaacgat tacgatttga gatgtctaaa tcaaaataaa aggagtataa aggtatatgg
      301 caaaaaagtc gaaaattgct aaagcaaaga aacaaatggc gatgattgaa aagtacgcag
      361 ataaacgtca ggaattaaaa gccgcaggag atcgcaccgc tctcgctaaa ttgccaagag
      421 attcaaatcc gaatcgtctg aggcttcgtg atcagacgga tggtcgtcca agagggtata
      481 tgcgaaagtt cggtatgtcc cgtattaagt ttagagaact tgcccatcaa gggttgattc
      541 ctggagtaaa aaaagcaagt tggtaaaagg aggaaatatc atggcagtac cagcaagaaa
      601 aacatctaag gcgaagaaac ggttaagaag aactcatcaa actgtagtaa aaccagaaat
      661 ttcatttgac gaagcgaatg gtgattatag acgtagccat catgtatctt taaaagggta
      721 ctataatgga aagaaagtaa ttaaaggagc ttcaaaatag tttaatatat aacttttagg
      781 agggaatcaa aatggaaatt atttacccac ctttagttga agaaggatta aagtattatc
      841 ttgaaacgac ccagcaatcg ctagataaat gcacttttta tcgttcgatg gtagagagag
      901 gaattattac tgaaacgggt ttaccgaccc aacaggcaat tgaaaatgga ttagtcaaag
      961 attactatga ggaccaaggc ttatcatttg atgatttttt aagaatctat ccaatttttg
     1021 aagagtatga cgaggagtta tttcaatgta ttgatggtta ttgggagata cctgttgata
     1081 tgaaagaaaa tttagtttca caattggaat ctggcgagtt aacttttgaa gatgcacaac
     1141 aaattcaagc atatcttgaa gatcgataag tattgaagga ggaagattta tgcgtcaaaa
     1201 tattattttg gaatgtgtcg aaactggaga acgtctgtat ctcaccagca aaaataaacg
     1261 gaataaccct gaacggttag aattaaaaaa gtattcgcca aaattgcgca gacgggcaat
     1321 tttcaaagaa gtgaaatagc taagtggtaa aaaggtgaca aaatgggcat accagttaca
     1381 atcgtttctg ggtttttagg ggccggaaaa acaacgctaa tcaatcaagc cttgcaagtc
     1441 tcaccctacc ccagggaaga aattattata attgaaaatg agtttggtga agttgggatt
     1501 gatcacaaat tattagttca tagagaggaa cgggtgttac aactaaataa tggatgtatg
     1561 tgttgcagtc ttcgcagtga tttattagca atattgattt ctttactgga aacaatagaa
     1621 gaacatcaac aaccaatttc acaaatcatc attgaaacta caggtatagc agatccccaa
     1681 ccaatcattc aaacactttt aacagcgcct caactaagag gaagagtata tatcgatagt
     1741 ttattgactg ttattgatag tgacagtttt gaacttattg aaagagaacc acaagccctt
     1801 aagcaacttg tcatggcaga tcgcattttt gtgtctagaa aacaaaactt gaatttatct
     1861 gcactaaaaa aattacagag agaaatcaaa agtattaacc ctttatccga tattcgaatg
     1921 ttcacgcata gtgaaacgat tcaaaaaaag gatttttata atttgaaaaa atttaatcat
     1981 cccttagttt ttgaaagtga ttcatcagaa aatcactata atggcaaaca ctatcatcaa
     2041 caccatcaag tacatgagtt taaatcaatt ctcttaactt cagatcaaga tataaaaaaa
     2101 gattttttac tccaatggat tgactggctg attttaacca accaagggag cctgtatcgt
     2161 tttaaaggat tgattgcttt acaagagcag gattttatag ttgccatgca aggggtaaac
     2221 gaacaggtta attttcaata caccactcga caaaaagacc aaaccactat tattctaatc
     2281 ggaaaaatgt tggatgaaga aaggatcaaa cagagttttg ataaattact ggcggaaagc
     2341 aaataatgat aagtattgat tgggggtaat ttctaatgga gaaaacggat atatctagtg
     2401 catatcgacg acttaaaagc ccgaatatta aaactagaaa aagggcatta aaaattatca
     2461 aagaacataa acgaaataaa atgaagaaat tagcataatt aaggaaggaa aaagaatgaa
     2521 gaaaatcagt gtaggactta ttgggatagc agctctagga ttattgggag cttgctccag
     2581 cactaatgat gcaaaagtat cgaatgataa agatggaaaa ttagaaattg taacaacatt
     2641 ctatccaatg tatgatttca ccaaaaacat cgtcggcgat gaagcgaacg tggatttaat
     2701 ggttccagcg ggatcagaac ctcatgacta tgagccctca gctaaggata tggcaaaagc
     2761 tcatgatgct gatgtttttg tatatcataa cgaaaacatg gaatcatggg taccaaaagc
     2821 taaagaaagt tggaagaaag ctgggcctaa tgtagtagaa ggaacaaaag acatgatttt
     2881 attgccaggt agtgaagaag aagatcatga tcacggagaa gaagatcacc atcatgaact
     2941 agatcctcat acgtgggttt cacccaaaat ggcaattaag gaagtttcaa atatcaagga
     3001 tcagttagtt aaattgtatc cgaaaaaagc aaaagtattt gagacaaatg ctgaaaagta
     3061 tttgactaaa ctgaaacggt tggatgctga ctataccact agtcttaaag aagcgaaaca
     3121 aaaaagtttt gtgacacaac atgcagcctt tggatattta gctttggatt atggattgat
     3181 acaagttcct attgctggac taagtcctga agaagagcca tcttctggtc gattagctga
     3241 attaaaagag tatgttaaaa aaaataagat caattatatt tactttgaaa agaatgctaa
     3301 tgataaaatc gcaaaaactt tggccaatga agctgggata aaattggaag tattgaatcc
     3361 tcttgaaagt cttacaaaag aacaaatgga taatggtgaa gattatgttt cagtaatgga
     3421 agacaatcta aaagcgttag agaaaacaac tatggttgct ggtgaagagg tagttccaga
     3481 aaaagaagcg aaggatgaaa agacagttgc taatgggtac tttaaagatg ccgatgtgaa
     3541 ggatcgcgaa ttgagtgatt atacaggcga atggcaatct gtttatccgt tgttaaaaga
     3601 tggaattttg gatgaagttt tcgattacaa agcaaaactc aataaagaca tgacagctgc
     3661 cgagtacaaa gattattata ctactggcta taagacagat attgatacta tcaacattaa
     3721 agacaacacc attgattttg tagtcaacgg tgaacatcat caatacacat acaaatatgt
     3781 tggatacaaa attttgaatt atgaaaaagg aaaccgtggt gttcgtttta attttgaaac
     3841 tgatgatgca ggtgctggtc gttttaaata tattcaattt agcgatcatg gaattgcacc
     3901 aagtaaggca gagcatttcc atattttctt tggtggagaa agtcaagaaa aactttataa
     3961 tgaaatgcat aattggccta ctttctatcc agcttcatta tctgagcatg agattgctca
     4021 agaaatgatg gcccattaaa atccaatctt tggaggaaat acatggtaag aaaagtatta
     4081 ttgtctatgg attccaagaa tattgatgct tatcggcgtt atcgatttgc aaagcaaatg
     4141 agattaaatg ggagtacatt agctgatatt gctgcaactt tacatgttag tgttgacaca
     4201 gttcatagag cattaaaaaa agatccagat tgttgtttta tcaaggttaa tactggaaga
     4261 agtagacgtt tagcaaaacg atataatatc ttgataaact aattgtaaca ctaaagcagt
     4321 tcaattcttc cttaatggaa tttgatctgc t
//
LOCUS       DAXWOO010000184         4310 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.188, whole genome shotgun sequence.
ACCESSION   DAXWOO010000184 DAXWOO010000000
VERSION     DAXWOO010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.188"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(65..1285)
                     /locus_tag="ACGE70_002562"
     CDS             complement(65..1285)
                     /locus_tag="ACGE70_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFF8694162.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1305..1916)
                     /locus_tag="ACGE70_002563"
     CDS             complement(1305..1916)
                     /locus_tag="ACGE70_002563"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFF8694163.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1959..2891)
                     /locus_tag="ACGE70_002564"
     CDS             complement(1959..2891)
                     /locus_tag="ACGE70_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFF8694164.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3312..4172)
                     /locus_tag="ACGE70_002565"
     CDS             complement(3312..4172)
                     /locus_tag="ACGE70_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFF8694165.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1230 a          866 c          707 g         1507 t
ORIGIN      
        1 agcttaaatc atagataccg taagggactt tattctttat ttaaaacttt gcaacagaac
       61 ctttttattt tacagaagca ctcgaaactg catctactgc agtttcatcg tgtgtgctgg
      121 cgctggttac tgcatctgtt tttccaccat gaacagatgc acttgaaacc gcatcttcat
      181 ttccaacaag ttgttgaccc gtttgttgta aataccactc aatgattggt ttgaaatcaa
      241 gaatgtattc attgattcca gcatactctc cattttcatc atgcattgct tggtaattat
      301 gcacaatata tttttctggc acttgttttg gtacatgaac acgtaccgca tctgttgctc
      361 cgcttcgcag ttgttgaatt acccactcta catttttata cgatttttct gggtgacagt
      421 tggctaatgg attccctacc tgaccgggca cacgagcagc taacatctct ttcgcttctt
      481 ggcgcaaatt ataatataaa aattggttat tattatctgc aaaagttaat tccatcggca
      541 tcgaatctaa aaagtaattg atttgatcca ctgtcaataa cccacgattt aatttgacgt
      601 aagtatttcc agaaacggca ccgactgctt cagaagcttt ttccaaccaa tgatcatctg
      661 acatatcaac atcgggtatt gtcgtagcaa ttgttcctgt cgcatgcaaa tcttctggtg
      721 gaagcttttt aggccctgct aaggtagaca cgactttaat gaattttaca aaacttgcta
      781 atgtactgcc taaaaagtca acagttcgtg gatcaatcaa gcgatcttct tggtcaaatg
      841 cttcttttgc ttttcccaat aaaaattcat ttccgggcat cactaatgca ttcacgcctg
      901 gtgcgtccaa aatttggcgc aagtgtaatt gcgcgcgtga agttccttga tcgtagtaag
      961 aagcacccac aaccattaca ggtttatttt caagtggatg gattttaaat gatagccatt
     1021 caagtgcact tttcaatcct gctggaaccg tatgattgtg ttctggcgta gcaataatta
     1081 ccccatcagc cattaagatt ttgcgattta gctgttgaat agcaggacta tttgtttgat
     1141 catcatcttg attgaacaga ggtaaatctt tgatttcaat gatttctaaa ttaaatagtt
     1201 catgatattg tttttggata aatgttaata atttacggtt atatgaaagt tcagctgctg
     1261 atccgacgat acctattaat ttcatctttg tttgctcccc tcgattaaat cgtttcccat
     1321 gaaaagcttt cggcttcacg tttatttgct tggtgcgcat gttgcaattg actagtgatt
     1381 gtgacaaaga gtaagaaatc tttgaatagt tcacgtaatt ttgcgacttg ttcttcatca
     1441 atgagtcgtc cttgctcatc aaaggcttgg agtgaatgac ccaataaaaa ttctgagctt
     1501 ggcataattc gtgcctttaa ctctggtgaa tccaatattt ggcgcaaatg tgcttgtgcg
     1561 cgtgaagaac ctaacgtgcc ataagacgct ccggtaatca tcactggttt atccacaaac
     1621 ggaaaaatac catatgaaag ccaatttaat gcgttaatca atgaagcagg aaccgcatgg
     1681 tcatactctg gtgtgctgat gattacgcca tctgcttgtt caatttttcc agcaatttct
     1741 ttaacaacat ttggtagttc tgtgttttca ggtttgttaa atagtggcag ctccttgatt
     1801 tcgacaattt cgatttctgc ctcatttaca aaataatttt tcataaactg taataattca
     1861 cggtttgttg accgcgttga atttgttcct actaatccaa taaatttcat cttcattctc
     1921 tttcctttct aacatcgatt tctgactgtt cgcacgtact aaattttttc aaatcctttt
     1981 gtatacaaga attgattctc ttttgttaca actgctccct cgatacccgg ctgttcttca
     2041 agcgctttca acacttctaa agaagatgct ccaaataaac gtgtagtcca gatttctcca
     2101 tctacagaag aatcagacaa aatcgtcagg cttgctacgt catttttaac tggaaagcca
     2161 gttttcggat caatgatgtg atgatacact gacgaaccat tttgtagata acgttcgtag
     2221 attccagagg taactaaaga ttggttgttt agtttaacca ccgcaagcga ctcttcacgt
     2281 gaacctttag gatttcgcaa accaatacgc caagcaccat ccgaacgtct tggtgattca
     2341 ccaaaaacaa cgatatttcc tcctaagtta actaaagcaa acgctgcatt tcgctctcgg
     2401 caatgtgtaa ttaatttatc tgcaatgtaa ccttttgcta acgcccccaa gtcaatcatc
     2461 attcctggtt tggttaaaaa aacggttttc attttttcat ctaattgaat ttggtgtgga
     2521 tccgttagca gaagtaactc agttatctct gcttttgttg gaaccaatgc atcttcaaat
     2581 ccaatgtgcc atttttgtac aaccggacca atagcaatat tcagataact atttcttgca
     2641 caactatgtg cttttcctaa tttgatgagc tcaaaaagtt caggatgtac aatcacagct
     2701 tgtcgtccag cagcttgatt gatggccatt aattcagaag ttgcatcatg tgcgctgaag
     2761 cgatgttcat aaacgtgcaa ccattcgcct aactctgcta gtaatcgact aggctcttca
     2821 tgcccaactt gtaatgtaat tgttgtcccc atcatttgcc agattttcgt ttcttcgatc
     2881 acctgattca ttttgtacct tctttacttg aactacggat tacatgagta attagttttt
     2941 atttttgtta ctgttttcac aaccagatga tacgcctgct agatttattt gtcaaccgat
     3001 tttatatatt tttttatata ataaggtaat tttaatataa aattttatat tgtaaaaaaa
     3061 caaataaggg aaacaaacaa cacatttttg tgtgttgctt atttccctct aactatcgtt
     3121 ttctatttga tggttaacct acttaaaata aatactgcaa cgacaaacgg agatcaagca
     3181 tgctttgtaa cgtattttac gataattacg actttatttt attactctat cttgctgcca
     3241 tacccgtcta tccctaatca tctttggtga attttttcaa agttagcata acaaaaataa
     3301 gtattttttc cttagttaat caatagtatc atttttttca tatgatttaa ttcatcaatt
     3361 aattttttgt ttttattaat tttagcaaaa gctagtgcat cttgaatcaa ttcgagcact
     3421 tctgccttcg acttattttt agcctgtgcg ttcttcgcta attgggcagc agctcgtgct
     3481 aagtaataag tcaaatggtt atccgaacaa atatcgattg cgtaatttag tagtgcatca
     3541 cttgtttcca aatcaccgtc ttgcgcaaaa aaaacaccac aatgaaagac gatatttaac
     3601 acccgccaga tatcttctgt tgtacgaatc ggatacgtat aaattttttc aaacactcga
     3661 ttgaaataaa cttctgcttt ttccaactct ttttctctag catagaccat tccaatacct
     3721 gtatatgcca ataaacgaaa caacgtgttg gctgtttctg ccatcaacaa ctgatcaaaa
     3781 tggaacaaca catccgtaat tggtgcattc gtaaaaatca tttcaaatcc ttttagatag
     3841 tagtaacgtg caatcagctc agactcgtca attaccttgg gatcaatggc tgctaaaatc
     3901 tgtgtcaatt gttcatattc actcgtaatc agtgaaaact ctgctttttc tagctgttca
     3961 tcaagttgtg tattgatcac gttcatcgaa gaaaaaagtt caactaacgg aagattgatt
     4021 cgattacata atttgattaa aatttttaac gaagggactt gaccattgtt ctcaaattta
     4081 cttaatgttg cttgtgtaca aattccttca gacacttctt tttgagataa gttggcagcc
     4141 tttcttgccg taataaattt ttcaatgttc attattttct ctccaatcag acctcacacg
     4201 aaaatcattt ctgtctcgat tttagcatgt tttctttggg aatgctttag gttctgttgc
     4261 aaagttttaa ataaagaata aagtccctta cggtatctat gatttaagct
//
LOCUS       DAXWOO010000185         4213 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.189, whole genome shotgun sequence.
ACCESSION   DAXWOO010000185 DAXWOO010000000
VERSION     DAXWOO010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.189"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            478..1446
                     /locus_tag="ACGE70_002566"
     CDS             478..1446
                     /locus_tag="ACGE70_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFF8694166.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1514..2272
                     /locus_tag="ACGE70_002567"
     CDS             1514..2272
                     /locus_tag="ACGE70_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFF8694167.1"
                     /translation="MYPGGNSLNFAVFAKKLGHESSFMGVFGSDEEAAHVLSAIKEIG
                     LDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREYPIQLTEEDREYLNQFELIH
                     MGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAIDKVDFGFFSIEDFSDEEVKQ
                     FLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEEPVDTMAAGDSFLTAFLLYF
                     LEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2340..3050
                     /locus_tag="ACGE70_002568"
     CDS             2340..3050
                     /locus_tag="ACGE70_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFF8694168.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3086..>4213
                     /locus_tag="ACGE70_002569"
     CDS             3086..>4213
                     /locus_tag="ACGE70_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFF8694169.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1411 a          631 c          892 g         1279 t
ORIGIN      
        1 ataaattgta ttaaaatcaa tgtatcactt tttaatcagt tttaagacca taaattgcgt
       61 actaaaagaa attctgcaat tcaaaataaa gcattttaag ccattttcaa atttaagtgc
      121 acccatctac ccgtcaaatc actttatttt ccactccagt agtttcctaa ttcaactgtg
      181 tgcaaaaacg atttcttcta aacaaatcaa gacaaactta taaaccttca tcccagcacc
      241 ataattattt aaatcatagg catttttcga aattagtata tgtactcaag tttcactgca
      301 gattatttat attttttaga gaaataaatt tttcctattg acaaacacaa agaaagcgga
      361 tacaatgcaa ttatgtcaca tgacgttata tgacgttatg tgatatcttt acatttaatc
      421 agttactttt attttggata aaattaacat ttttaaaatt ttaaggagga aatgttgatg
      481 gatgtagcaa aaacggttaa cgcaattgta gaaaaaaatg atggagagat ttctggaatt
      541 tatttggtgg cttgtggcgg ttcgctggta gatatgtatg tctcagatta ttttcttaaa
      601 gccgaagcat cgaagtgtat tacaggctta tatacagcga acgaatttaa ccatgtaacc
      661 ccaaagcaat tagatgataa atctgttgtc attctatgtt ctcatggagg aaatacagca
      721 gaaacggtaa aagcaggaga agtcgctcaa gctagaggag ctgtgactgt tggtctgact
      781 cataataaaa ctgcggattt attaaaggtt tctgatcata gtttccttta cgaatgggga
      841 gatgatgcaa aaatagtcaa taatcctatg gccatcattc ttgatttaac tgtatctttg
      901 gtcaatgtta ccgaaacatt tgaacattac ggtaaattca gagaaggcat gaaggttatc
      961 gataccgtaa ttgaaaatgg cattaagcag gtgcaggatc gtattaaagt gtttgctgat
     1021 aagtacaaag atgaaacaat gttttatatc ttaggtagtg gaccatcata tggacatgct
     1081 tatggttttt caatttgctc tttgatggaa atgcaatggc ttgattcaac ttctgttcat
     1141 tctggagaat tcttccatgg accttttgaa gtaacagaca aagatacgaa ttttattctc
     1201 ttgatgagca ttggacgcac tcgtgcttta gatgaacggg caaaacgatt ccttgataaa
     1261 tatgcagaaa gagtagaaat cgttgatgca aaggaactag gattggatat gatatcagat
     1321 gaagtttctg aattcttcaa tcccatttta ttctacagta ttctttcaga atatcgttca
     1381 gctttagcag atgtacgcaa tcatgattta gatgttcgtc gctatatggg gaaagtggag
     1441 tactaggagg attaaaatga aggtattagg cttaggtgat aacgtcgtcg ataaatatga
     1501 aaatctggga attatgtatc ctggaggaaa ttcattgaat tttgcagttt ttgcaaaaaa
     1561 actgggacat gaaagtagct ttatgggagt ttttggctca gatgaggagg cagctcatgt
     1621 tttatcagca ataaaagaaa taggactgga caacagtcac tctcgctttg aaaaaggcga
     1681 gaatggctgt gccagagtga agattgatga gggcgaccgg attttccttg gcagcaattc
     1741 gggaggtgtt actcgtgaat acccgattca gcttactgaa gaagacagag aatatttaaa
     1801 ccagtttgaa ttgattcata tgggattata tagccatgtc aatcatctat tagaagagtt
     1861 acaaaatgtt tctggaaaga tttcttatga tttttctgac gactttacgg aagaagaggt
     1921 tcagcgggca attgataaag ttgattttgg cttcttttct atagaagact tttccgatga
     1981 agaagtaaag cagtttttga tgaagcattt ttgtgagcga aatgatgtat tgattgcaac
     2041 ccgaggagaa aaaacggcta ttggttatga tggagcaaag ttttatgaag tgaaccccgt
     2101 gctagaagaa ccagttgata caatggcagc gggagattct ttcttaacag ctttcctact
     2161 ttattttctc gaaggggacg atatagtcac cgcgatgaaa aaggggaatg aatttgctgg
     2221 gaagtcttgt ctggttgacg gttcgttcgg ctttggtatt cattacgatt gagcttgttt
     2281 taaattcctg catttgataa cctaaagaaa gaaatcccgg taaagtggag gttaaaaaaa
     2341 tgctggatac aaatgcacaa ttaccattgt acttacaatt aaaagaaata ttaaaaaata
     2401 aaatcaatac aggggagtat cccgagaata cgaaaattcc cgctgaatca gagctgatag
     2461 aaagttatga ggtcagccga atcacagtga gaaaagcagt agaagaactg gtagaagaag
     2521 ggtacctact taaaaaacaa gggaaaggaa cttttgtcaa ccggcataaa gtttttcgta
     2581 aaattgaata tattatgggt ttcagtgaga gttgtaaatc taatggtttt acaccgacta
     2641 gcattgtttt ggaacgcaag atcattccag caactgaagg gatggcaaaa aaactcgata
     2701 tcgctcaagg tgatgatgtt ttatatatcc agagaaagcg aatggcggat aacatcccca
     2761 ttatgttaga aaacaattat ttttcttatg aaagatatag tttcttaatg gaggaagagc
     2821 tgactgattc tttgtatgaa gtacttaaag tccaaggtac cttagccatc aatcctgggg
     2881 aaactgcact tgaaattgtc acagctgatg accaaattgc caaaataatg gaagtagctg
     2941 ttgggacgcc ctttttcttt atggatactt taatcaacga tcaaaacaac ataccaattc
     3001 atgtaggtaa tcaatattac ctgggagaag tatatcgttt ttacttatga tttcctgttt
     3061 ggatttcaag gagtagattt tatttatgaa gcaaatattg cggtcaaaaa tgatttttga
     3121 tggagagcag ccacctttca aaggttatat tgtaatcaat gaagggatca ttgaaaaggt
     3181 ggaaaacaac tggaattaca gtgatctatt aaatgaaaac acggaattaa ttgcttatga
     3241 tgatcaattc gttatgccag caattcatga taatcatgta tttttcagtg gttatttggc
     3301 tatgaatgct ggcatagatt taagcgatac atcttcagct aacgaggctg tagagcgtat
     3361 taaagctgta atgaaccata aaaaagcaga tgaaccaatt tatgctcatg gctgggatgc
     3421 tgccctgtgg aaaactgacc catcagaaga gctattgaat gaaataaacc actttggtcc
     3481 aatcacagcg atcgacaaaa atcgcagcca ttgttggatg aatgctattg ctaagcagac
     3541 ttatggtttt acagaaaaag aaaccagtgc tgaaagtcga gtattattaa ttaaggaaat
     3601 gctagcaaac aaaaagcttt tagcagaggt ttacgctgaa tttgaagcat tactgctttc
     3661 aaaaggtgtt atatcaatga aagaaatcgt ttttgatgac gcagaatttc tttctaatat
     3721 acctcagagg aaaatactta gtaacctata tgtgcaggca gtccaagaac ctctacagca
     3781 tgaacttcta tatgagtata aggaaaaagt tttccctgaa aaggtccgct ttggtggagt
     3841 gaagatcatg gtagatggtg ttgttgcgga tagaagtgga gatatttacg gtgtttatca
     3901 gtcaggagta gcttcacccg agattgacta tgaggcaatt aaagcagaag ttgctgcatt
     3961 caatgaagta gagattcctt gctgtttgac aacggaagga gataaagcgg gttataaagc
     4021 tgcgtcaatt cttgtcgaat ttggaaagcg gttacctgaa ggcgttttta attcgattag
     4081 tgatttggaa atggttactc aaaagacagc agaaaaaatg aacgaaggac aagtagtagc
     4141 agaggtatat ccacaaattc tcggcttgaa tcaaacgtta gaagaggctt acatgtcaac
     4201 tgttataaaa aat
//
LOCUS       DAXWOO010000186         4207 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.190, whole genome shotgun sequence.
ACCESSION   DAXWOO010000186 DAXWOO010000000
VERSION     DAXWOO010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.190"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            1..329
                     /locus_tag="ACGE70_002570"
                     /pseudo
     CDS             1..329
                     /locus_tag="ACGE70_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324676.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(601..891)
                     /locus_tag="ACGE70_002571"
     CDS             complement(601..891)
                     /locus_tag="ACGE70_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694170.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1293..1529
                     /locus_tag="ACGE70_002572"
     CDS             1293..1529
                     /locus_tag="ACGE70_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694171.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1536..3488
                     /locus_tag="ACGE70_002573"
     CDS             1536..3488
                     /locus_tag="ACGE70_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFF8694172.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3560..4057)
                     /gene="dfrG"
                     /locus_tag="ACGE70_002574"
     CDS             complement(3560..4057)
                     /gene="dfrG"
                     /locus_tag="ACGE70_002574"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFF8694173.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
BASE COUNT         1436 a          742 c          715 g         1314 t
ORIGIN      
        1 gcaaaagaaa tctacgatag tcaacaaaaa aattttcatt atatccagaa aaatttttct
       61 tagttaagga cctatggatt gcagtaatgc agaattcgtc gaataaactg ccaaagacat
      121 acaatcatat tgcttttaaa attgacgaac aagatataga ttcgtttgtc tcaaaaatcc
      181 aaatgttagg tttaactgtc gaaccaggac gttctcgtgt gaaaggcgag gcttcttcaa
      241 tttactttta cgactatgat aatcacctct tcgagttaca taccggtact ctccaggaac
      301 gtttaaaatc ctacaattcc actacctgag actagtaatg actagtctct ttttatggcc
      361 tctgatcccc ccattgagcg aatactaata gggcttcgaa ttgatctgtt caatctttaa
      421 gcaatatccc tcaaatgagt ttggaaagtt tagtgattca tcacaaatac ttaaatacat
      481 gtagttatat atagattgag gttcttgtga gcgtatgctc gtgtgggttc aaatcccatc
      541 gtctgcatcc atctttccaa aacaagcggc taggacattt ttgtctcagc cgcttgtttt
      601 ctagtttatt ctttcatctt gtcggacctt ttttcttggg tatagacttt tctcatattc
      661 taccacaatt ttgggtttcc ctaacacatg ataggatctc acaacaaatt gctttctatt
      721 attataataa cctgtaacga ctaacttcat cccttcgtct acttcagcta ggaagttcaa
      781 actatgggaa gcaatcaaac aattgacccc atttaaactg aaacggatca acggtctggc
      841 actaacttgc aaagtacgga tcttatctac tgttccatat tttttctcca taaccttcac
      901 ctcaatacca ttatacgaac atacattccc tttgtaaaga agaaactctg ttttatcttt
      961 ttgacttttt gtctattacc tttcttaaaa tcatattggc acttcctgcg aagatgaagg
     1021 acatgtacta aaaaaaagag gcagcagtat agatttaagt agttcggttt tggagtacaa
     1081 aaagtgaaaa tgtaaaccta aatgagtatc aaattgcaac tgaatttgat actcgttttt
     1141 tattgttaaa tcaacgtttg ttgttaattt acaccacaaa atgtaagtta aaatgtatat
     1201 aaaaatgcga acgtgaaata attattgtag gaatgcaaat tggtgtgtta aaatggttac
     1261 aatttactaa gcaaaaaggg gtgcattttc ctatgcaatt tcttgagcca aaaaataaga
     1321 acgctaagtc tgtggattgg gaaatatcag agcaagtaag ggtgatagtt aagcaatacg
     1381 ctgaatatgc agagcgtact gaaagcgaag cagtggatga attcttattg aacattttag
     1441 acgataagaa gtttattgaa tggattgcca acaagcgaag taataagcgg attgttgaaa
     1501 aaatgggtat caaagataga gtgggttgat agacaatgac caagcttaaa agattagcca
     1561 cgaaggaaga tgaaattgta gatgttaaaa ttccgatttc aaacgaagaa ttaaaagata
     1621 gggctaaaca atatgattta ttaactccaa aacgatttgc aacaaggtat aacaaaatgt
     1681 tgttcttgcc aacgtctttt aaatggaacg gttctgaata tcccattcaa tataattact
     1741 gcatcaatcc gttttgttgt aattttggga aagaacagca caaattcaaa gatgtaaaag
     1801 ggaaaccaag tcgatataaa atgactggta gttccaaaga taaaggtcat aaaggtatgt
     1861 actgcaatga caatcctatt ggcagaggtg tatcgcaaaa ctgtacagta acccctttat
     1921 ctaattggtc tgttgttgaa gaaattaaac gccttataga aatcaatagc atccaagatg
     1981 ttgagccaga ttatcaattt cataaagaag gctgctcgga agaagaatca accccattca
     2041 atgagccgaa gcaattttac aaaaggggga aaagtagggg caaatctcaa cgctatcaat
     2101 gtaaggcgtg taagaagttc actaatgttc taccaaaaag agaagaaacc actacttatc
     2161 accaacaaaa gaacaccatt cttccaatgt tcgctaagat ggttgttgga agggtttcag
     2221 tcagtcgtac ttgtgatatt ttaggaattg gagttggaac ctactatcat aagctggagt
     2281 ggctgtatag gcgttgtttg gagtttttag aacgatatga gacacaaccc cttcaaacaa
     2341 agaaatttaa cgagatgtgg cttaatacgg acaagatgca ctactaccta aataacgtcc
     2401 gtaagaaagg acaaggttca aagaaatata cggggtttga agaccttaat atgcaaacgt
     2461 atatagttgt atctgctgag gtcttatcaa gatatgtgtt ccgttctgat gttgcctatg
     2521 attggaatat ttcaatggat gaattaaatg aagatacaag aaaattcaaa gaagaccatc
     2581 tcaatacttt tagtcgtaaa aatgataggt tagattggtc ttattatcca caagaaccat
     2641 ccgctaacga tagcgaaaat aggaatgctt atcttcacga attagggaaa attactaata
     2701 ggagtagatt tgtcgatggt ttaaatgttg atgctcctta tactacaact gctcactatt
     2761 ggttaattaa acaaatggtt aatgccgacg aatggcgaat gattagtgat gatgacttct
     2821 ctattagaaa cgccttttat agagtattca cgaaagaatt aaggttgagc gatgcacatc
     2881 attttatttg ccaagttaac aagacgaaaa gcagaaaaca atgcttaaaa gaattcggac
     2941 aagcaaaagc agaactgctc gattggggag atatcagagg attcaaaaca aagtttctac
     3001 ggactttggc atctcattat ctaactgaat tactaacaag tcatcaattc cacgaagagg
     3061 ctattagtaa agatggagaa agatatcgca aatatgccga taaccctatc aaacatccat
     3121 tagccactaa ggataaagga ttttattcag ttgattgtag aacggattta tctgctctgg
     3181 aaccaaacga aatcgcaaag atgctattaa atgttaatga ccattcgacc aatagcttta
     3241 ttcaacagat aagacgttat atatcatctc ttgaaagacc attaacaaca gctcgtggtg
     3301 ataagaagag ctatatttac gctaacttca atccgaagta tgctcaattt gcgattacaa
     3361 ttctaagaac ttactacaac ttttgtcgcc cttttaaatc agcggataaa aaggttttga
     3421 cacctgctca aagattaggg attacagata aacaatttga ttggaaagat ataatttatt
     3481 tcaaatgaca aaaagagctt ggagtaatcc caagctcttg tgctttatct ttactgttct
     3541 tcttcaatta acgcacccgt taactcaata agttttttct ttcatataca tgaaaatagt
     3601 agttatacgg atttttatca ttctttatcc ctttttgggc aaatacctca ttccattcct
     3661 cataattcac ttctggaaaa aaagtatctc cttcgaattc atgatgtatt tttgtgatgt
     3721 acattttctc aacataaggg aaaaacaaat tataaatctg ttctcctccg aaaataaaaa
     3781 tttcttcttc gtttttacat aactcaaaaa catcttctat tgaatgaaca atttcacaac
     3841 cattaaaggt aaacccctta tctctcgtca gaataatatt tcttctgtca ggtaaggctc
     3901 ttccgattga ttcaaggttc ttcctaccta atattatcgg atgtcccttt gtagtatttt
     3961 taacatattc ccagtccttg ggaatcctcc aaggaatgtc attctctttg ccaatcactc
     4021 tattcttatc catcgcagca atcaaagaaa ctttcattta taacacagcc tttttaacta
     4081 atttaccatc taatttctta tttctatcaa aaaacctttt ctcattatcc tgcccgttag
     4141 ttgaataagc aattttttct ataagttata ttatcataat accacaaaat cacaaatttt
     4201 cattcaa
//
LOCUS       DAXWOO010000187         4169 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.191, whole genome shotgun sequence.
ACCESSION   DAXWOO010000187 DAXWOO010000000
VERSION     DAXWOO010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4169
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.191"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(31..1203)
                     /locus_tag="ACGE70_002575"
     CDS             complement(31..1203)
                     /locus_tag="ACGE70_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HFF8694174.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIGNSHNRL
                     KSTNLLERLNQEVRRREKIIRIFPNRTSANRLIGAVLMDLHDEWLSSTRKYIKFDQ"
     gene            1304..1810
                     /locus_tag="ACGE70_002576"
                     /pseudo
     CDS             1304..1810
                     /locus_tag="ACGE70_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1968..2585)
                     /locus_tag="ACGE70_002577"
     CDS             complement(1968..2585)
                     /locus_tag="ACGE70_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFF8694175.1"
                     /translation="MAKIGYARVSSLDQNLDRQLELLSECDKIFSDKASGKDTNREGF
                     QEMMKFIREGDIVVVTELKRLGRNNKELTETMNLIQLKKATLEVLNLPALTGITDDNL
                     RRLLNNLIIELYKYQAEEERRYIRETQQQGIALAKAKGKYKGGTLKYRENDPRLQLAF
                     NLFLTGSTDKEVEELTGINRRTFRRYRMKHGITNEVREQKNKSFS"
     gene            2849..3229
                     /locus_tag="ACGE70_002578"
                     /pseudo
     CDS             2849..3229
                     /locus_tag="ACGE70_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            3229..4026
                     /locus_tag="ACGE70_002579"
                     /pseudo
     CDS             3229..4026
                     /locus_tag="ACGE70_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
BASE COUNT         1356 a          767 c          732 g         1314 t
ORIGIN      
        1 taaaatacta tacaatgttt taccggtctc tcattgatca aacttaatat attttcttgt
       61 agaactgagc cattcgtcat gaaggtccat aaggacagct ccaattaatc gattggcaga
      121 cgttcggttg ggaaaaatcc gaataatctt ttctcttctg cggacttcct ggttcagtcg
      181 ttcaagaagg ttggtgcttt ttagccgatt atgactattt ccgataaccg tgtattgaaa
      241 ggcatcttcg aagccattat ccaatatttc gcaggctttt gtatatttag gctggtcgat
      301 atattcaccg actaaggcat tcttagctgt tcgagcgagt tcaatatccg taaacttaaa
      361 gatcgctttt actgcttctc taaacggttt tgaattcttt tttggaatgg aactgaagat
      421 gtttcttaaa aaatggacct ggcatctctg ccaacttgcg ttggtaaatg acttacgaat
      481 cgcagacact aggcctttat gggcatcaga aatgatgagt tctgtcccct ttaggccgcg
      541 ttcttttaag tattcaaaga agatggacca tgtgtcatca ctttcttcat tttgaatcat
      601 gaagccaatg atttcacggt cgccaccttc tgttatcccg atcgcaatat ggcagctttt
      661 agaaagcact cgatggtcct cacggacttt tatgtagaga acatcagtca tcagataagg
      721 ataattcgta cctgagagtg aacggttctg ccattcgttg accatcgggt ctaactgctc
      781 agtcaggcta gaaacaaaag atttcgatac agattttcca catagctctt caacaatctt
      841 tgaaacttta cgtgtcgaaa cgcccgaaac atacatctca agcattgaag cgagcagtgc
      901 cttttcattt cgctgataac gctcaaacac cgtcggtgaa aattcaccat cacgtgttct
      961 aggcactttt aattcgagtg tacccacacg agtcgtaaag tctcgctcat aatagccatt
     1021 tctttgactc tgacgacttt ctgaacgttc atagtcatcg gcttgaatat attctgttcg
     1081 ttgattttcc atcaattggt tgaaaacagt ggttaaaata tttttagaaa catcatcttt
     1141 taccgaatgt tcaataatac tttgaacctc ttcgttgttc agtgtaaaat gtacttgggt
     1201 catgtaacgt cctcctgggt atgtttttgt ggttaaaaac attgtaccgt aaaagggctg
     1261 ttacatggcc ttttatcttt tacacaatta tatggacttt atcgctttgc aatggaaata
     1321 cattgatttt gaaaaccaaa cagtccgtct taaatatgcc ttggataaac acacaaaaaa
     1381 gaaatttaca aaaggtcgaa aagatactat tatccaagtt ccggaaattg taatgacact
     1441 attgcaagaa tggaaatcag tgcaagctga acaactattg aagttgaaaa ttaaacagac
     1501 acctgaccaa tatcttttta gttactccaa accttctggt gaagtaaatt gtccagtcca
     1561 tactgattac ttaaattatc gaattaattc tataaaaaaa agacatcccc atttggttca
     1621 tttaactcct cataaacttc gacatactta cgcaacttta gctagacaag gtggggctga
     1681 tatgaatcaa atttctaacg ctttgacaca ttcaggtatt tcaactacaa aaatttatgt
     1741 gaatacacct gatttagttg ataaagctgt gttcgaagcg tttcagcgag gattacaaaa
     1801 atctaattaa tgtgttctag aatgtgttct aaatataaaa aaaagacact ttccaaataa
     1861 ttggaaagcg tcttgaacgt tgatatataa aggatattaa cgttttgaga attgagatgc
     1921 tttacgagct ttcttaagac ctggtttctt gcaaaattat attagcctca actgaaagat
     1981 ttattttttt gctccctaac ttcattagta ataccatgtt tcattcgata tcttctaaac
     2041 gttctacgat taatccctgt tagctcttca acttctttat ctgtagatcc agtcaaaaac
     2101 aaattaaatg ctaattgaag tcgcggatca ttctcacgat attttagcgt acctcccttg
     2161 tatttccctt ttgctttagc gagtgcaatt ccttgttgtt gcgtttcgcg aatataccga
     2221 cgttcttctt cggcctggta cttgtatagt tcgataatta agttattaag taatcgacgt
     2281 aaattatcat cagtgatacc tgttaacgct ggcaaattca aaacttccag tgtagctttc
     2341 ttcagctgaa ttaaattcat cgtttctgtt agctctttat tatttcgtcc aagtcgtttt
     2401 aattctgtca caacgacaat atcaccttca cgtatgaatt tcatcatttc ttgaaatcct
     2461 tctcgattgg tatctttccc acttgcttta tcactaaaaa ttttatcaca ctctgataat
     2521 aattctaatt gtcggtctaa attttgatct aagctgctta ctcgagcata cccaattttc
     2581 gccataataa aactccttta aaattctgaa atccggatca tttttaaatt ctgttgccca
     2641 actctgttgt catttttcta gataaatggc ataaattttc agcgctaagt gacaaaagca
     2701 actaattttt catgatagat ttttaaatat ctcaatctat ctccaaaatt agttgctttt
     2761 gtagctatag ttgactatga atatgacaaa taattagaaa aaactgtgcc aattaaatat
     2821 caaaatgaca aattgatgaa tcaaattcaa attaaggggg gcaaccctgg aagtcttaaa
     2881 tttaccctca atgaatggta ttgaagatga aaatttaagg cgtttgatta atagccttgt
     2941 cattgaattg tacaagtatc aagcagaatc agaacgaaaa aaaattaagg aacgtcaggc
     3001 acaaggaatc gaaattgcta agaaaaaagg caaattcaaa ggtcggcaac tgaaatttaa
     3061 aaaaaatgat ccacgtttaa agcatgcttt cgatttgttt ttgaatggtt tatccgataa
     3121 agaagttgaa gaacaaactg ggatcaatcg ccgaacgttt agaaggtatc gagcaagata
     3181 taacgtgaca gtagatcaaa gaaaaaacaa tgaaaagagg gatagttaat gagtacggtt
     3241 attttagctg aaaaacaagc caggcattag cctacgcaag tgctttaaaa caaagcacca
     3301 aaaaagacgg ttattttgag atcaaagacc cactatttac agatgaaacg tttatcacct
     3361 ttgattttgg gcatttagtg gaattagcag aaccaggtca ttatgacgaa aagtggcaaa
     3421 attggaaact tgaatctttg ccgatttttc ctgatcgata cgattttgaa gttgcaaaag
     3481 ataagggaaa gcagtttaaa attgttgcag aacttctcaa aaaggcaaat acaattattg
     3541 ttgcaacaga tagcgacaga gaaggtgaaa atatcgcctg gtcgattatc cataaagcaa
     3601 atgccttttc aaaagataaa acatttaaaa gactatggat caatagctta gaaaaagatg
     3661 tgatccgaag cggttttcaa aatttgcaac caggaatgaa ttactatccc ttttatcaag
     3721 aagcgcaaac acgccaaatt gccgattggt tgatcggcat gaacgcaagc cctttgtata
     3781 cgttaaattt acaacagaag ggcgtacaag gtacattttc actaggacgt gttcaaacgc
     3841 ccaccttata ccttattttt cagcgccagg aagctagtat aaaagtaaaa caagggtcat
     3901 ttaagggcgt tctaagcccc acacagcgtt ttaaaaccca agaggagctt ttttgctttt
     3961 gtttcttcta aacaagctaa aataggcaat caagagggga taattgctga tgttcaaacc
     4021 aaagagatgg ataggctaca attaactaga cagaaaaatt aaggtgtgta gactagaaga
     4081 aaatatacca ggaggaattt ttatgtctaa gagaacacga agaacttttt cacaagaatt
     4141 caagcaacaa atcgtcaatc tttacttag
//
LOCUS       DAXWOO010000188         4117 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.192, whole genome shotgun sequence.
ACCESSION   DAXWOO010000188 DAXWOO010000000
VERSION     DAXWOO010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4117
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.192"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(18..497)
                     /locus_tag="ACGE70_002580"
     CDS             complement(18..497)
                     /locus_tag="ACGE70_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFF8694176.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            856..1638
                     /gene="rpsB"
                     /locus_tag="ACGE70_002581"
     CDS             856..1638
                     /gene="rpsB"
                     /locus_tag="ACGE70_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFF8694177.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            1737..2618
                     /gene="tsf"
                     /locus_tag="ACGE70_002582"
     CDS             1737..2618
                     /gene="tsf"
                     /locus_tag="ACGE70_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFF8694178.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            2754..3476
                     /gene="pyrH"
                     /locus_tag="ACGE70_002583"
     CDS             2754..3476
                     /gene="pyrH"
                     /locus_tag="ACGE70_002583"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFF8694179.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            3479..4036
                     /gene="frr"
                     /locus_tag="ACGE70_002584"
     CDS             3479..4036
                     /gene="frr"
                     /locus_tag="ACGE70_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFF8694180.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
BASE COUNT         1465 a          724 c          890 g         1038 t
ORIGIN      
        1 aggaatacgt gctctgatta aaaaagtgat acttcagttg gatggtgaag aaattcttct
       61 actcgttttg cgtcctcctc attttccgag atgaccatga tcgtatcgaa tccagcaatt
      121 gtactgacaa tagagggagg tttgatctca tctaaaacag atgcaaataa atgagcattg
      181 tctggcaagg tattgacgat cgtcaaaaag tggactcgtt cgactttcaa tacaatgtct
      241 tcaatcattt gaaccagccg atcctcttcc tcagtcctag tctcaggagt tcctttttcg
      301 cgaaacaata cgaagcggga aacgccattt tcatcaggtg ccttcacgat ttttagatca
      361 cggatatctc gagaaatcgt tgcctgcgtg gcggtaacgc cataatgccc aagtttgttt
      421 aatagttctt cttgcgtacg gatcgggaat tcttcaatga cttgtttaat cagcaaatgt
      481 ctttgagctt tgcgcatttt ctttctcctt tacgaaagat ttcacatgta tcataacaaa
      541 aaaaagaagg attgcccaat aatattcgtc ttttatacct aaaattattt tatggaaaaa
      601 ggataataaa gaaacaaaga gaaatctact gtttttcttg aattcttttt atctttcagg
      661 taaactattg aatgttgagc agaaaagtat atttgctctt gcgatacgga tcttttgtct
      721 gtataatagc tgagggtgct ttatgcatca gcaaatccac attcttattg gattgtcggt
      781 aggtgcttga aaaagtttgc cggcaaggta gaagataaga agaggaaaaa taaaaccaaa
      841 tcggaggaaa caaacatggc agtaatttct atgaaacaat tactagaagc cggcgtacac
      901 tttggtcacc aaactcgtcg ctggaatcca aaaatgaaga aatatatctt cacagaaaga
      961 aacggaatct acatcattga cttgcaaaaa acagttaaat tagtagatgc agcttatgat
     1021 tacatgaaga gcgttgctga agaaggcggc gtagcattat tcgtaggtac aaaaaaacaa
     1081 gcacaagaag caatcaaaga agaagcaact cgtgcaggac aatacttcgt taaccatcgt
     1141 tggttaggcg gaacattgac aaactgggat actatccaaa aacgtatcag ccgtttgaaa
     1201 caaatcaatg caatggaaga agatggaact tttgaagttc tacctaaaaa agaagttgct
     1261 ggtttgaaca aagaacgtga acgtcttgaa aaattcttag gcggtatcgc tgatatgcca
     1321 agaatcccag atgtaatgta catcgttgac ccacgtaaag aacgtattgc tgttcaagaa
     1381 gcacacaaat taaatatccc aatcgtagct atggttgata caaactgcga tccagatgaa
     1441 atcgacgtag taatcccttc aaacgacgat gcaattcgtg ccgttaaatt gattacttca
     1501 aaaatggctg atgctttcat cgaaggaaac caaggtgaag atcaagtagt tgaagaagat
     1561 ttcacagaag aaaacacagc aacatcaatc gaagaaatcg tagatgttgt tgaaggcgac
     1621 aattcttcag cagaataatt ttgaatgatg gagctgtttc aaaggctggg cgacaagcct
     1681 cactctcctt tgaagcagct ttttttaaaa agaaaaacac acaggaggag ttaaacatgg
     1741 cagacgttac agctaaaatg gtaaaagaac tacgcgaaat gactggcgta ggaatgatgg
     1801 atgcgaaaaa agcattagtt gaagtagaag gcgacatgga aaaagcagtt gatcttttac
     1861 gtgaaaaagg aatggcaaag gctgcgaaga aaaatgaccg cattgcagct gaaggattgg
     1921 cttcagttgc tgtgaaaggt aacactgcag caatcgttga agtaaactca gaaacagact
     1981 ttgtttctaa aaacgaaatg ttccaagatt tagtaaaaga aattgcagaa cttgttgctg
     2041 aaaacaaacc agcagacatg gaagctgcaa tgaaaatcaa aacagataaa ggtacaattg
     2101 aatctgactt gatcgaagca actcaagtta tcggagaaaa aatcagcttc cgtcgctttg
     2161 aagttgtaga aaaagacgac aatgctgcat ttggtggata cttgcatatg ggcggacgta
     2221 ttgccgtatt gactgttcta gatggaacaa ctgacgaaac agttgcaaga gacgtagcta
     2281 tgcacgtagc agctatcaat cctcgttatg taaacgaatc acaaattcca gaagctgaat
     2341 tagaacatga aaaaactgtt cttacagaac aagcattgaa cgaaggcaaa ccagcgaata
     2401 tcgttgaaaa aatggtagaa ggacgcttga agaaattcaa agcagaaatc gctttggtgg
     2461 accaaccatt cgttaaagat cctgatatga ctgttgaaaa atacgttgca tcaaaaggcg
     2521 caactgttaa aacattcgta cgtttcgaag ttggcgaagg tatcgaaaaa cgcgaagata
     2581 actttgtaga agaagttatg agccaagtga aaaaataatt ctgttgaata acagacggga
     2641 acgcacggtt tgtgcgctcc ctttttttaa gcaaaacaag aaaaaccagt cttagagtgt
     2701 cgaaatcatc taagtatggt aaaatggcaa tagaaaatat tggaggaatg aacatggtca
     2761 caccgaaata tcagcgtgta gtcttaaaat tgagtggaga agctttagct ggggatgcag
     2821 gatttggaat caaaccacca gtaatcaaag aaatcatcca agaaatcaaa gaagttcacg
     2881 agctgggcgt tgaaatggca atcgttgtgg gtggcggaaa tatctggcgc ggacaaatcg
     2941 gagcacaaat gggcatggaa cgtgctcaag ctgattacat gggtatgtta gctacagtta
     3001 tgaatgcctt agctttacag gatacgttgg aaaacgtggg tgtaccgaca cgcgtgcaaa
     3061 cttcgattga aatgcgtcaa atcgcagagc catatattcg ccgtaaagca gaacgtcatt
     3121 tagaaaaagg acggatcgtt atctttgcag gcggtaccgg caacccgtac ttctcgacag
     3181 acacaacagc agcattacga gcggcagaaa tcggcgcaga tgttatcctg atggctaaaa
     3241 acaatgtaga cggtgtttat tctgctgatc cgaaagtaga tgcaaatgca gtaaaatttg
     3301 aagaactgac tcatctggaa gttattgcca aaggattaca agtcatggat tcaactgcaa
     3361 gttcactaag catggataat gatattccac tgcttgtctt caatttaaat gaacacggaa
     3421 atattcgccg tgcaatctta ggcgaaaata tcggaacaac tgtaaggggg aaataaaaat
     3481 ggcagatgca attatgacag aagcaaaaga aaaaatgaaa aaagcagcac aaaatcttca
     3541 acgtgaactt gggcaaatcc gcgcaggccg tgcgaacgct agccttttgg accgtattac
     3601 tgtgaactac tacggtgcgc caacaccatt gaaccaaatg gcatcaatcc aaattccaga
     3661 agcacgtgtt ttaatgatca caccttttga caagagcatt cttcaagatg ttgaaaaagc
     3721 tattatggca agtgatatcg gaatcagtcc aaccaatgat ggaaatgtca ttcgcttagt
     3781 tatcccgcaa ctgacagaag aacgcagaaa agaattagct aaagatgtga aaaaagaagc
     3841 agaaaatgca aaaatcgcga ttcgtaacat ccgtcgtgat gcgatcgatg aatataaaaa
     3901 acaacaaaaa aatggtgata ttaccgaaga tgatttgcgc ggattagaaa aagacgtaca
     3961 aacattgaca gataacagca ttaaagaagt agataagatc gctgctgata aagaaaaaga
     4021 attattggaa gtttaataaa atcataaaaa cagaggggcg gatcattccg cttcctctgt
     4081 ttttatttat atttctttta tttaaaaatt aaaaaaa
//
LOCUS       DAXWOO010000189         4083 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.193, whole genome shotgun sequence.
ACCESSION   DAXWOO010000189 DAXWOO010000000
VERSION     DAXWOO010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4083
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.193"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(30..488)
                     /locus_tag="ACGE70_002585"
     CDS             complement(30..488)
                     /locus_tag="ACGE70_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFF8694181.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(501..1832)
                     /locus_tag="ACGE70_002586"
     CDS             complement(501..1832)
                     /locus_tag="ACGE70_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFF8694182.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1843..3285)
                     /locus_tag="ACGE70_002587"
     CDS             complement(1843..3285)
                     /locus_tag="ACGE70_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFF8694183.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3272..4036)
                     /locus_tag="ACGE70_002588"
     CDS             complement(3272..4036)
                     /locus_tag="ACGE70_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFF8694184.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
BASE COUNT         1227 a          885 c          603 g         1368 t
ORIGIN      
        1 tcaccctatt taactttttt catcgtacac taaaattcaa aacaactatc tttaatcttt
       61 acttcttggt tttgttttaa taaatttcct tcaaaaatgc ctggagaaac tacaatgatc
      121 accgtatctt tttgtgcttg ctccaacttc ttcaaatcta taatagctaa cagtgtccct
      181 tcttttacct cctggttact ttcaacccgt atctgaaacg gacttccttc gagttcaact
      241 gtatctaaac ccatatggat cagtattttt ctgccagatt cactttcgat cgttatggca
      301 tgtcgggtag gaaatatatt atcgattatt cctttaactg gcgcatggat atatccatca
      361 tgattcgtta tagcgtatcc aactcctatc atttttgaag aaaagacctc gtcttttact
      421 gctgttaatg gaataatttg accactagct acagctttta gaatgtttct tgttttaaag
      481 aaatgcactt tttatcacct ctactttaat gctggccaat attttttgtt tactttgatc
      541 atttcatcta gtattttttt tgcgactctg gcatttggta cggtttgatt caatgtaaat
      601 gcttgtaaag ctttttgata agaaccttca aagtaagcat ctactagtaa cttctctact
      661 gcaacttgtg cctccatcaa tcctttatga aaatcactga ttggttttct tagtgtaatt
      721 ggctccacac cagttgcacc aacatatgcc ggtatttcaa caactgcatc tgaccgtaga
      781 ttcggaatcg ccccttgatt aggaacgatt agcataaagc gacgacgatc atcattcaac
      841 aaggaaatag ctatatcgac gatatattgt ccatgctccc caaaattaaa gttcaatact
      901 tcttccacaa taccgcggcg tattttatca gccatttctt tggtattttt ctctcgtcca
      961 tccatgacca tattagcacg tgtatactct ttgtcagtat gctcaacgac catatcagga
     1021 tataagtaat actctaagta attattcggg atattacttg gaaaattttt cgtgatgact
     1081 gacatcattt gaaaggtttt ttgccaagtt ttatctccta tattaaaatc ggcgacttgc
     1141 atttcttttg ttattagttt ttcaataatc tctggcataa tttctcgttg caattcttta
     1201 tcataaatcg atgtatacca gccaaagtgg tttaaaccat aatacgtaac gatccaattc
     1261 ttacgatcat aaccgtaatt cactgcaatc gtttcttcaa ttgaaatagt catatcacag
     1321 gcattaatca tttttacatt cggatattta cgacgaacag cttcagaaac gatcgactct
     1381 ggatttgtat aattcaaaat ccatgctttg ggggcatagt cttgtatatg atcgacaatt
     1441 tccaataaac ctttgattga gcgcatccca taagcaaatc ctccgagtcc acacgtttct
     1501 tgtcctacta atccatgttt aagtggaatc ttttcgtcct tttctcgcat ctccaatcct
     1561 ccaacacgaa tttgtgagaa aacaaaatcg catcctgtaa aagcaagctt tggatcatcc
     1621 gttgcacgaa tgcgaatatt tgaaaaacct tttcttttca gcataaattt aacaatcaag
     1681 tacatatcca tatttctttc ttcatcaata tcatataaac gtatttcaga taatggcaac
     1741 cgtttttcat tgttcaaaat ggcttgaacg attcctggtg tatacgtact cccgcctcca
     1801 gctatagtga taatctgttt tttcatcatc atcttttcct ttctattctt ctaataattt
     1861 attgaattct gccataacat aagtcacttc taagccgatg ataatttgat aatttttttc
     1921 agttggttta atgatcccgc ttatccctaa ttgcttgatt cgctgttcct cgaccatggt
     1981 gggatccact acctctaccc gtaggcgtgt attacaacta tagacatcca ggatattggc
     2041 ttttcctcca aacgcttgta gcatttttgt tgccatatat tcataatttt tacttgctaa
     2101 ccctaactct gtttcttcta aggttacttc attgccaaac tctgccttag cttcacgtcc
     2161 taatgtctga atgtttctct tattgatcaa ataataaaaa gtaacgtaat acaaggcaaa
     2221 aaagaaaatt ccgattggaa gaatcaaaat agcattggta gcgattcgaa aattgatgag
     2281 ataatcaaca atacatgctc cgaatgtaaa accaattcgt atattgaata agtaacagac
     2341 aatccctgct aaaccggcaa aaagtgcatg gatcacgtac aacataggag aaagaaacat
     2401 gaaactaaat tcaacaggct cagtgatatt tgcaataaat gaagttcctg caccgcttcc
     2461 catgattcct ttgaccatct ctttattttc tttataagct gctttataga tagctagaca
     2521 tatagctggt ataccaaaca tcattactac aaaaaaaact gataaaaaca gtccagcctg
     2581 aggatctccg ttgataaagc gtgtcatctc tcctctgaaa acttccttgc taggagatgt
     2641 atagcttcct aaatcgtaat aaatatacgt attcaacaca tggtgcagtc caaaggggat
     2701 cagcaaacga ttcaaaaaca taaatatccc cacacctaat gctcccatat caacgagcag
     2761 ttttgcaaaa ttatcaatcc caatttggat gagcggccag ataaagccca aaaaaacagc
     2821 tgcgattgtt tgtagaatca tcaccatagt caatgggaat ttttcgcccg caaaataaga
     2881 aaaaactact ggtaattttt gttctttaaa gcgattatac gtccatgccg ctactaaacc
     2941 ggaaataatt ccaccaaaaa tccccatatt cacttctggg tccataattt ctaagccttt
     3001 tttcaaggta gcaatcgcta aaaaaccagt aagtgcaggt attcctttgt ccttattttt
     3061 gacaaaaccg attgcaactc ctatagcaaa taagatatcc aagtttccaa aaacggcatt
     3121 tccagctgca ccaattactg ggatgttcag catatcgtca gctgataaac gcatgatcaa
     3181 tcccgctaat ggcatcgctg caactggaag tagcatcgct cttccaagct tctgcacatt
     3241 tgatttaaca ttcattttta ttcctcctct attaaatatc ctcatatgtt actaactcaa
     3301 tcaaaggatc atttttcact ttttgagcaa atgctgaatg aattaaaaat tcgacttggt
     3361 gggtacgagg atacacaaaa cttgaaccat ttaacaattc ctgatccaaa tacgctgcgt
     3421 gacacatgac ttcgatcgtt ttatcttctc tcaatatcat gagtaaactt gccaaatatt
     3481 cttccacact cgtattgatt tgtcgttttt tttcatcagt ttctcctacg acatcgaaac
     3541 aaggctcaaa ataatcgaca tgacgaactt catcttttcg ttcaaagttg ctccgaaccg
     3601 gcaaatcata tttttttgct aatgtaacaa ctacctcctg attttcatcc aaagtatgag
     3661 tatgatgatg actgtctaaa tgagttggta tgatccccgc atgataaatt ttttgaatct
     3721 gagcattcca ttcctgatac agttgatctt tgtcaatgtt aaatggatgc tgataataag
     3781 ataaggatct gaactctccg ctttcgataa gagtatcgac agttttgagg agtggtttgc
     3841 cacacgtcaa tgttaaatgg actcctacac ctagcctagg catttccttt cttagtttca
     3901 cagcatgctc gaaacccggc atatttgcca tcaatgtagt tgatgtcaaa attcctcgct
     3961 gataagcatc cataatccca taattcacac catggctata gccaaaatca tccgaattaa
     4021 aaatcacttt tcccatttca ttctccttct tttttctact aaaataggcg caaccaaata
     4081 atc
//
LOCUS       DAXWOO010000190         4072 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.194, whole genome shotgun sequence.
ACCESSION   DAXWOO010000190 DAXWOO010000000
VERSION     DAXWOO010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.194"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            66..1490
                     /locus_tag="ACGE70_002589"
     CDS             66..1490
                     /locus_tag="ACGE70_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFF8694185.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(1671..2444)
                     /locus_tag="ACGE70_002590"
     CDS             complement(1671..2444)
                     /locus_tag="ACGE70_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFF8694186.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(2441..3346)
                     /locus_tag="ACGE70_002591"
     CDS             complement(2441..3346)
                     /locus_tag="ACGE70_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8694187.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(3500..3820)
                     /locus_tag="ACGE70_002592"
     CDS             complement(3500..3820)
                     /locus_tag="ACGE70_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFF8694188.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         1223 a          750 c          819 g         1280 t
ORIGIN      
        1 agtaaaaata aaaaaggttt gttatacttt ttgataaaag cggttttatt ttggaggatg
       61 cttacatgga tacagagcaa cagaaaaaag gaattggttt aattccactt gctgccttag
      121 taattggttc agcgattgga ggtggcgtat ttggtattat gacggacatc tccggatcgg
      181 ctggcggccc agctattttc agttgggtac tcgtaggaac agcaatgttg atgctctcct
      241 tgtcgatcaa taatatcaat aaaaaacgtc cagatttaaa aggcgggatc tttagttacg
      301 cagaagaagg ttttggccgt ttcgctggtt ttatcagcgg ttggggttac tggctcacat
      361 gctggttagg caacgtagct tttgcaacat tgttgatgag cgcattagga tatttcttcc
      421 cagtatttga aggtgggcaa aatctggcat cgatcgtgat gagtacagtc tttttatggg
      481 gatatgcatg gctagtgaat cggggagtcg aaaatgctag tttcgtcaat gctatcgtca
      541 cattctgtaa attagtgcca ttatttttat ttattgtagt agctatcagc acgttttcga
      601 tccaacaatt cattgataac ttttctagcg acattatatt gaatgcgaat ggacagccga
      661 tcccgttttc cagtcaagta ttgagttgca tcatggtgat gctttgggta tttgttggta
      721 ttgaaggagc ctctgtcgta ggttcacgag caaaaaaaca atcagatgtt gggaaagcaa
      781 cgattctagg gatattcggc ttgattatta tttacgtctt ggtttccatg cttccatatg
      841 ggacaatgac gatgaatcag ctgcaaacga tcaaggcgca gcctgctatg ggctatgtgt
      901 ttgaaatgat ggttggtaaa tggggagcag tcgtgatcaa cggcggtttg atcatttctt
      961 tagttggtgt ttggctctca tggacgatct taccgattga aacaatggga aacatggctg
     1021 aagacggact tttgcctaaa gcatggggaa aactcaacaa aaatggggca ccaacttatg
     1081 ctatcgtatt gacgacgtta tgtacgaatg tctttttact ttcattatta ttcactgaca
     1141 gtgcttataa ctttgcttac acacttggta cagcagctat tttcttcacg tggcttttct
     1201 taggattgta ccaaatgaaa ctttcttatc agcggaaaga atgggtccaa tttttagtag
     1261 ggcttttagc aagtgccttt caagtatggg ctatgctagt cgtagccttt aatgaagtaa
     1321 tgcttgttct tgttcttttc ttgccaggca tttattttta cctcaaagca cgtaaggagc
     1381 aaaacgagcc tgtacaacat atccacttag ataagatttt acttataggt gtgacgatgg
     1441 tcggagtgat tgcaatggtt ttatttgcca ctgggatgct tcacgtatag aagtataaaa
     1501 gaagtataaa gagaggttgt gagcctgtcg tttattcaaa aacatactag gaaaggagct
     1561 gtgacacaaa tgttgtcaca gctccttttt cagaataaac ggtgtttaag agacagccaa
     1621 tcggcaataa ggctgaacgt ctctttcaca gcctcttttt tgatgagtca tcatacattt
     1681 tttcgcttaa atacgagata tcctaatgct aagaacaaaa cgatgtaagc taaattaccg
     1741 aatagcattt gattagtaga aagatgagtc aattgcttca tgatctcttc gtttccaatt
     1801 tggttttgca gattcaacat actgatcgga ttccatttga tccactccca tttttggatg
     1861 agtgcaataa ggataccaga tatgattgac gaagcaaaat aaaagacgat tcctgctgag
     1921 atggatgcgc cggaactatt gataaagcaa gcaatcatca acactaaact taaaatcagc
     1981 cataaaccga tataactacc caaagtataa gtgaacaagc tttggatcag tgtttgacca
     2041 gtagaaacct tctccgtgaa ggaaatcgaa gggaaaagaa tcagtttcat caacacagtg
     2101 acaataatag ctaaaaggta taaataaaca gaataaataa ctaatgtgat ccatttgctt
     2161 actaatattt ctcctcgaga atactttcga taaaggagat ttttcaatgt accatattgt
     2221 gcttccatcg aaatgatcgt gcttgcagct gcaatcatga taaagacaat ccaagatgtg
     2281 gcactaaaaa gctgagcagt catttcaact ggatcaatga tcgtcgtgta ttttttggct
     2341 agaagtgctg ttcctatgag caaaacaact aatagaacag ataaaacagc agttgacttt
     2401 ttcttgatca atttaaataa ttcttgtttt acaagcaatg tcatttttct tcttccccct
     2461 ttactgaatc cagaatttcc aatagagaag attccaaatc attcgtcaga tgctggacgt
     2521 ctgtcacatg gatattttgt gcagacaata gctggatcac ggttcctaat aattcttcgt
     2581 tttcttttaa aagcttgatt ttatcaggat cttccaaagt atatccagct tctgtcaaga
     2641 tcgttcgagc agctagatca tttgttgtag agataacgat gaaatcttgc gctgtttggt
     2701 tcaattcagc cattgtacat tgtttgatga tctttccttg attgatcagg atcagctcat
     2761 cgaccatttt ttcaagctcg ctgagtaagt gactggagat caagaaagag gtgccctgag
     2821 tagccaagtc ggtaatgatt tttcgcacat ctcttgttgc ttgtggatcc aaaccgttca
     2881 tcggctcatc taaaataacg atagacggat ggttcagtaa ggcaagagca attcccaatt
     2941 tttgtttcat tcccaacgag taggatttcg cttttttgtg aatgtacttt tccatcttga
     3001 gctgattcac gatagtgtcg atagcagata catcgttggt ttcagagaat aacttcagat
     3061 gatcatagcc tgttaagaat ggatagatac ccgggtattc gatcaatgct cctacttgtt
     3121 ccaacccttt atgactagag ggagaaatct cattttcacc aatacagatt gttccatctg
     3181 agtaatggat cagtcctaaa atagctttca tgatcgttgt ttttccagcg ccattaggac
     3241 ctactaatcc aacgattttg ccaggggata agctaaaact gacatcgctg agggcttgtt
     3301 tgtgaccaaa gttcttggat aattcttgga ccgaaagact tttcaaaaat attcactcct
     3361 tttctataga tagtacaagc atacacctga tgagtggaga agtcgctagg tcattggact
     3421 tatttcttat tttttcataa aaaaattgaa tattaaataa aaaggcttat tttttctgat
     3481 cagtacttat tccaagtgtc tagtggccgc tagtaatctt gatgccaatg atactgatga
     3541 gtagcatccc cacaaagaac caagtggaaa gattcagctt atcatgaaac aagaccacac
     3601 caattatgac cgaaccaact gcgccaatcc ctgtccagat aggataagag agactcatcg
     3661 gcaaactttt gattgccttt gacaaaaagt aaaaactagc aatcatgcca atcactgtgt
     3721 aaaaagaata attgatttta gaaaaaccat cactccattt catcatcgtt gaccagaata
     3781 cttccaaaat tcctgctatg attaattcca tccatgccat acttacattc ctccttaact
     3841 ataaaaaaat acacaaaaag aggaaaatca tcccttcaaa gacctaatgg aatgatcatc
     3901 ctctttatcc tacccggtgg cagattgtat atctttttct gtagtatagc acacctatcc
     3961 agatatcggc aatctcatac tttttagtaa agtgtaccaa gcactgacgg atatatcgtt
     4021 agcggaaaag aagatgaatg ctttgcaatt tcatagaaag aagttgttat tt
//
LOCUS       DAXWOO010000191         3984 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.195, whole genome shotgun sequence.
ACCESSION   DAXWOO010000191 DAXWOO010000000
VERSION     DAXWOO010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3984
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.195"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            164..313
                     /locus_tag="ACGE70_002593"
     CDS             164..313
                     /locus_tag="ACGE70_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694189.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            394..990
                     /locus_tag="ACGE70_002594"
     CDS             394..990
                     /locus_tag="ACGE70_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFF8694190.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPERTLCDIWNPWYTVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            1003..1902
                     /locus_tag="ACGE70_002595"
     CDS             1003..1902
                     /locus_tag="ACGE70_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFF8694191.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNEIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKERKLAYQRHQQMNREMER"
     gene            1905..2390
                     /gene="ssb"
                     /locus_tag="ACGE70_002596"
                     /pseudo
     CDS             1905..2390
                     /gene="ssb"
                     /locus_tag="ACGE70_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            2412..2669
                     /locus_tag="ACGE70_002597"
     CDS             2412..2669
                     /locus_tag="ACGE70_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694192.1"
                     /translation="MAKKISLTMDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVF
                     VYLVCSYLLKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(2828..2950)
                     /locus_tag="ACGE70_002598"
     CDS             complement(2828..2950)
                     /locus_tag="ACGE70_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HFF8694193.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            3105..3364
                     /locus_tag="ACGE70_002599"
                     /pseudo
     CDS             3105..3364
                     /locus_tag="ACGE70_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328871.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3812..>3984
                     /locus_tag="ACGE70_002600"
     CDS             3812..>3984
                     /locus_tag="ACGE70_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFF8694194.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPYTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGD"
BASE COUNT         1416 a          695 c          804 g         1069 t
ORIGIN      
        1 tgtacgtctg aagcctgtga ttttaaaagg tcagaagggt tcatttgaag caacgattgt
       61 tttaaaagaa gacttcacta caggctttga attttctgaa aagaaaaaaa cgaacttcaa
      121 gaaaagaaca cgccgcactg caaaataatg gagggttaat cttatgcgta ataccacaca
      181 caaacaacaa gaaatcgaaa atcaaaaagc caaaattcgt tcagaacagg aaaaaatgcg
      241 tgccagcaaa cgaagactgc agcaattgaa gaaccagcag aggattcaaa aagaaatcga
      301 attagatcga taaaatcttc ctaactattg tatacgtata aacttatgtt atactgtttt
      361 tataagttta tacgtataca aaggagggag aaaatgacaa aagcaatggt atacaagctg
      421 ctagaagaat ttaatggaac tttggctttg aaggatgcca aaaaagcggg catttctcct
      481 gtaacgatca aacgtatggt agatcgaggg gagctggatc gagagtatcc aggttttttc
      541 accttacctg gtcaatttcc tgatgaattg tttatggcgc aaaaaaaata tgaacgcggc
      601 attatttcac atattaccgc gttagatttg tatgatttaa cagatatgat ccctagacag
      661 atcgatctga ctgtacctta cggttatcac gtttctgaaa aaggattgaa ggaattcgca
      721 gttgagctac actatacaaa agcggaatgg tatgaattag gtaagattga aataaaaagt
      781 cgctatggga atcctattat agcttatgat ccagaaagaa ctttatgtga catttggaat
      841 ccctggtata ccgttgagga tgaaatcaaa gtaaaagcaa tcaaaaacta tatggagtcg
      901 gacagaaaaa atcttagaaa attgaatgag tatcgacgaa ttttaccaac agacaaaacg
      961 atgcgttctt atattatggc tttaaattaa aggagaagat acatgacacc tacgcagtta
     1021 aaagcaaaag tgaagaatat ttctagagaa aaagaagttg atccgcagct tgttttaaga
     1081 cattttatga tggaaaaatt cttggaaaaa atctcagagt ctccttatcg tgaaaatttt
     1141 gtgttaaaag gcggcttctt gattggctca aagtatggca ttgaaaatcg cacaaccaaa
     1201 gatattgata cgactttacg ggaaatgaaa gtaaccaaag aaacattaac aacggttcta
     1261 aacgaaattt tttcaacgcc gacgaaagaa ggcattcaat ttgagattca aggaatgaaa
     1321 gaaacgagag aagccgatta ctatccagga tttagcttac gtgtgctggc acatttggaa
     1381 aacatgcggc cagattttaa ggtagatgtg actacaggtg actcgatcta tcctgcaacg
     1441 atcactcaca gtcataaatt gatgtttgag gatcgaacga ttcctttgga atcctatcct
     1501 acagaacaaa ttattgctga aaaattaagt gcaacctttg attttttaac agataattcg
     1561 agaggaaaag atttttatga tctctatact attcctaaaa tggagaagat cgatgagaag
     1621 accttgaaag atagtgtgag aaatacattt acacgtcgag gaaaaactga tccgttaaag
     1681 atatactatg aaaaggatat ggatgtactg aaaaatagtc acgagctaaa agatacctgg
     1741 aagaaatatc aaaaaagcaa tccttatgct gcgtcgatca cgtacgatga gacaatggat
     1801 tcgattagta atttgatgga aaaagtgatt cacgtcgaag aaaaagagcg aaaactagct
     1861 tatcaaagac accaacagat gaatagagaa atggagagat aaagatgatt aataatgtga
     1921 cattagtagg acgcttaacc aaagatcctg atttacgtta taccgcaagt ggtactgcag
     1981 tagcgacttt taccctagcc gtcaaccgaa atttcacgaa tcaaaacggc aattgagaag
     2041 ccgattttat taactgcgtg atttggcgca aaccagcaga aacaatggcg acattagcta
     2101 aaaaaggtag cttgattggt gtagttgggc ggattcaaac gcggacttac gacaaccagc
     2161 aaggacaacg tgtatatgtg actgaagttg tagcagataa ctttcaacta ttagaaagta
     2221 aagcagcgac agagagccgt gcgcacgctg atcaatcgtc caccagtcca agcaccgcca
     2281 cctttgaaca acggaataca gcgacgccta acaacaatgg attgaacgct tcacagaatc
     2341 catttggtgg acaatcaatc gatattagcg atgatgatct accgttttag tttgagaaag
     2401 aaggagtaag catggcaaaa aaaattagtc tgacgatgga tctattgata cccgtaattg
     2461 catttataga cgggatttct ttgggcgtca ctgaaaatcc tttggtaaat agaattgcga
     2521 aaggatcact atttcttgta ttcgtgtatc ttgtctgttc ttatctttta aaaagagcag
     2581 gaaaggataa ttccttttgg cgattgacta aagtcacagt agcagcgaca gctgttatac
     2641 ttacggctat tctaccgctt tatatgtaga gttaacggga aatcgttgaa cttcttgcga
     2701 aatattgcta taatgaacat gaaaaagagg agttgcgcta acaacccctc attaacagca
     2761 ccgattaaag aacggtggca gaatattagt tatttggaaa taaccgctga cctgctaaag
     2821 tagacggtta tttctttttg tcatttttaa gcatatttac aatcaaacta atcaaagcaa
     2881 tggtaaacat accaaaacca agaatggttt gaattgtctc atatgcggac aaaaggctta
     2941 ctcctttcat agattttagc acttacattc ataagcacca ccacctttcc agaagtcgcc
     3001 accgtcttca aactttactg tcttgactat tatagcatat ctaaaatcga ataaaaattc
     3061 tcaattttag ctgtttttgc tataatttta gcgaggtgat cgtgatggaa caatatattg
     3121 gtttactcga taaattaggg tgatcaagac acagccattg cttgtgcgat ttacattacg
     3181 cacgagtcat gaaccaatca attgtgtagt ggccaatata gaaatagcgg ataaattgct
     3241 gattttggaa gaaggcaagt atactatttc agtgacaggt cacttcaata aacgaaatca
     3301 actagtcatt gaatcaatgc aaattagaaa ccctgatcat tttacgagat taatgggcat
     3361 ataaataaaa gaagagctgc agtcctaaat gaggattgca gctctttttg ttttacatag
     3421 aaacaagaaa aatgaaaagg cctttttagg aaggcgcaag cgccttctca attgaaaaac
     3481 ctctctctca ctccaaccgc caccgccgtt cgagaagaaa ggaagtgaac gttgcacgtt
     3541 cactaagata aggggtcttg caaagcaaga caaaatagaa aaaacttcct ctcaccaaaa
     3601 ggttcgttcc tgttcgctat ctctttattg atttgccaga ccggcaacaa gttgcctttt
     3661 atcaccaaaa agcgacaagg acaagggtaa atggcacgct gcacgccctt gtccttgtcg
     3721 cttttagttg attcattctg gccaacaaat caataaagaa agaactaacg aaaggaagta
     3781 aaacctttcc taagaggagg cagaatcaat catgcgaaag aaatttgatt attggggtgt
     3841 tcccttcagc gaattattcc caaactatca tgcaccatat actgtcgaat gcgattgtgg
     3901 agaaagagcg aaatgtatca aaagctattg tttgtatcaa tgtccaacat gcgggaaaaa
     3961 atataccttg tcttatggcg atta
//
LOCUS       DAXWOO010000192         3972 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.196, whole genome shotgun sequence.
ACCESSION   DAXWOO010000192 DAXWOO010000000
VERSION     DAXWOO010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3972
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.196"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(81..1586)
                     /locus_tag="ACGE70_002601"
     CDS             complement(81..1586)
                     /locus_tag="ACGE70_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFF8694195.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1583..2269)
                     /locus_tag="ACGE70_002602"
     CDS             complement(1583..2269)
                     /locus_tag="ACGE70_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFF8694196.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2462..3883)
                     /gene="gndA"
                     /locus_tag="ACGE70_002603"
     CDS             complement(2462..3883)
                     /gene="gndA"
                     /locus_tag="ACGE70_002603"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFF8694197.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1092 a          848 c          683 g         1349 t
ORIGIN      
        1 catccctgaa tttctcactt gcttgtattt tgtaccttat tctttgttgg atatataaaa
       61 aaacaagtaa agcacatgct ttacttgtct tctgcctttg cgataggaat aaagattcta
      121 aaaattgtcc cttgccctac tacactttct gctaagatac gtcctttata gttttcaacc
      181 agttgtttag cgatagacaa ccccagacca tttccaccct tcgttctagc tcttgcttta
      241 tccactcgat aaaaacgatt gaagatctta ttaaggtctt ctttgggtat tccttcgcca
      301 aagtcctgaa tcgcgatctc aaattcattc agagtggaag aaatagagat atggacttct
      361 tttcgtgtag ttgaatactt tactgcgtta tccaatatga taatgattaa ttgttcaaag
      421 tgattacgat aaatttgcag ggtcacttct tttgtcaaat catcatctaa agtgatcaca
      481 tagtctggat ataatatctt gaaattatta taaacttgat aggtgacttc ttttccttta
      541 gttgtttcat tcgcataatg aacatctact tgttctgctc ttgataagtc tagcatctct
      601 tgaactaagc ttttcatacg gctgatttct tgcatgctgg cgcttaatga ttcttctaat
      661 atctcaggat catctttccc ccaacgattt agcagactaa gatgcccttc aataattgca
      721 acaggtgtcc ttaactcatg agatacgtct tccacgaatt gctcttgttg ttcaatatac
      781 aaacgcatac ggtcaagcat ctcattgaat atttcagcta aatctgccaa ttcatcattt
      841 gtgtcgattt caggcatgtg gatatctgat tgcggatctt tacgtatagt atccattgta
      901 tctctgagta ccttgagtgg ttttaagaaa taagaagata aaaagaaacc taaaatacta
      961 cttaagatca aagaaacaac ttctaaaaca ataagagtca ataatagctt atttcgaata
     1021 tcatagaaag aagataattc atagaagctt tgcgcatagc cgatcttttc tcttgtacct
     1081 ttagaatata taggctggat agataaaaag ccagtttttc cttctagtgt aacgatcgtt
     1141 ggaactttac gtgtagtctg aatcaggttt cgactgtttt catgggtttt aaaaatcaag
     1201 ttctcgtcca aatcataaac ataaagatcc attgatggct gccctagctc agctataaag
     1261 gaatcgatct tcattaagtt tccttcgacc agtgaagtat gatctacgac ctcatctccg
     1321 tcatttgtcg ctttcgtcag attacgatac gtatttacta atgtcagttc ttcatccgag
     1381 ttagccaaac gagaagctac ttctgaaatc gtacgctcta cattgtttct ttcttttgcc
     1441 acaatcagat tgatagatga tttataggta atgacagcga aaattgtaaa cacaacgaat
     1501 atgaagaaag aactcgcaaa ggcccacttg attgtcaagg agggcccttt cagttctctt
     1561 ttttccgatt ttttcggctt tatcacgagc gcatcacata acctgttccg cgtacagttt
     1621 ggatatagct ttcctccccg ggaacgtcaa ttttgttgcg aagataacga atatatacgt
     1681 ccactacgtt tgtttcaact tctgtttcgt atccccacac tttatttagt aaaacgtcac
     1741 gtgctaatac cacgttcacg ttttccatta aagtaagcag caactcgtat tctctttttg
     1801 ttagttcaat tacttcattt ccgcgacgta cgacacggtt ttctttttcg atagtcaaat
     1861 cgcgataagt aatagtagtt tgtttagcca catttttatc gccttcaata tcgattcgac
     1921 gcaacaatgc acgaagacgt gctaataatt cttcgatagc aaatggtttg acgatgtagt
     1981 catctgctcc gtggtcaagt ccagatacac gatcgatcac agaatctctt gcagtcatca
     2041 taatgattgg tgtatttttg acttggcgta cacgtctgca gacttctaaa ccgttcaatt
     2101 ctgggagcat cagatccaac aatatggcat cccactcatt atttaatgcc gcatctaaac
     2161 cagttcggcc gttgtagtgg acttctgcgt tatacccttc atgcttcaac tctagctcga
     2221 caaatcgtgc caagttcttt tcatcttcaa tgattagaat attgctcatt tattatttta
     2281 tcctttctat tctactggct ttttaaaaac tctatcatta taccgataat atgcttaaaa
     2341 atctatagtc attttatcat ggatagcaat tttaaaaaag agctgaagtc tactcataac
     2401 aatcgacttc agctctttcg tcctcttttt attttctcat ttcctaacag ccgaaagact
     2461 attattcttc atggtaccat gagtaatgga agattccttc tttgtcgaca cgctcatagg
     2521 tatgtgcgcc aaagtaatcg cgttgggctt ggattatatt tgcaggcaga cgttctgctc
     2581 tgtatgaatc gaagtaagcg atggctgatg agaaagtcgg tacaggtacg ccagcttgga
     2641 cagccaaggc aacgacgtct ctgactgatt gctgatattt tttcgtgatt tcaacgaagt
     2701 aatcatctag caataaattt tcgatctcag gatttttgtc ataggcatcc gtaatttttt
     2761 gcaagaatcg agctcggatg atacatccag cgcgccagat ttttgcgatt tcaccaaatg
     2821 gcaagtccca atcaaattct tttgaagcga cacgtaattg agcaaatcct tgggcataac
     2881 tcatgatttt actgaaatac aacgcttctc ggattttttc gaccaattct tttttgtctc
     2941 ccgtgaactc aaaatcgttc gtacgagaca aaatcttgct tgcttgtacg cgttcttctt
     3001 tataagcaga aatatagcgg gcaaatacag attcagtgat caatggaagt ggcacaccta
     3061 agtcaagcgc gctttggctt gtccatttac ctgttccttt attcccagct gcatcaagga
     3121 tcacatctac tactggttta cctgttcctt catcgtcttt acgtgtcaga atgtcagcag
     3181 tgatttctat caaatagctg tctaattcgc cttgattcca ttctttaaag atatcagcca
     3241 tttcttctac tgaaaggttt aaaatattct tcattaagtc ataagattca gcaatcagtt
     3301 gcatatctcc atactcaata ccgttgtgta ccattttcac ataatgtccg gctccatttg
     3361 ggccaatata tgtgacacat ggtgctccgt cttctgcttt tgcagaaatt tgctctaaga
     3421 ttggtgcaac tagcgcatag gcttcttttt gtccaccagg catgattgaa ggtcctttta
     3481 aagctccttc ttcaccaccg gaaacgcctg taccgataaa gttgatgcca gagttggcta
     3541 actcttcatt acgacgcatc gtatctttaa agaatgtatt tcctccgtca atcaaaatat
     3601 cgcctttatc aaggtgagga agtaactctt ggattgttgc atctgttgcc ggtcctgctt
     3661 tgaccattag caagatccga cgtggtttct cgatagattc tacaaattct tcgattgtat
     3721 atgttgcttt gaaattttta tctgggtgtt cttctactac agcggttgtt ttagcccctg
     3781 tacgattgaa taaagccact gaatagccgc ggctttctat gttcaaggct agatttttac
     3841 ccataacggc cataccgaca acgccgattt gttgttttga catatgaact cctcctacat
     3901 acgggatttc tccctaatct cttacattat agcttaacac ggctacattg caaactaaag
     3961 aacaacatat tt
//
LOCUS       DAXWOO010000193         3862 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.197, whole genome shotgun sequence.
ACCESSION   DAXWOO010000193 DAXWOO010000000
VERSION     DAXWOO010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.197"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..665
                     /locus_tag="ACGE70_002604"
     CDS             <1..665
                     /locus_tag="ACGE70_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694198.1"
                     /translation="AYYLLSEPKQIAASNIFTAVMTLMCVMCFSIFSYLLVQFIRQNL
                     TMSKLFKSLLDLLILGSMLTTLTDRFEQSEKTVEGIKNFLTTIDQFINGYMYLFEVGF
                     ILTLFGCVFIKLIKRSIEKEMVIDLIPTLSGYEYYVDFGKYEELIHTDIVHTSIHEVT
                     VKGIRSTLKDGQETKTIDQRKEVYNFYKVEASLRYGIGKLRLDGSNIQQNKPYVLGKV
                     VI"
     gene            709..978
                     /locus_tag="ACGE70_002605"
     CDS             709..978
                     /locus_tag="ACGE70_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694199.1"
                     /translation="MILLYRIVLIVGTELKILKKEQLFKVKEHHNQLLCIKTGTMNIQ
                     YRTEYLELTNQTDFLSIREQHKQEKLMATYSQLIDEWEQFKESKT"
     gene            1210..1506
                     /locus_tag="ACGE70_002606"
     CDS             1210..1506
                     /locus_tag="ACGE70_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694200.1"
                     /translation="MNVKIRDLDPKFISEIDRRCVELSNRTGNKWSRNDYLKLLVEND
                     FDRPLMEYKQEKFDQLLEKFSEIQSYNTKILEEYVSQNNRIIEILIEQNRGSEL"
     gene            1508..1930
                     /locus_tag="ACGE70_002607"
     CDS             1508..1930
                     /locus_tag="ACGE70_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694201.1"
                     /translation="MAKVIDKHIYFHQTHIDVLDETISTHAEVKNYSEAVRFLCMNYD
                     PEVEKKVNSNQAKLNGMSKEISILTEMVSEFADLHLKDTGILIGKNSTVYQEAKERVE
                     GKIKSNQTKKYSPQLKKPVNESVHARVDPFDPTKILKR"
     gene            1949..3541
                     /gene="mobP2"
                     /locus_tag="ACGE70_002608"
     CDS             1949..3541
                     /gene="mobP2"
                     /locus_tag="ACGE70_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobP2 family relaxase"
                     /protein_id="HFF8694202.1"
                     /translation="MTQSPSVILTCQFTLPNKKSFSTYIDYITREQALEEKERRTPEE
                     EQELQRIKNALEEFYIPEGETFSESKTGKEFSETSNEAQSLMEHGINFDKLEEQDFTN
                     YLSYMTRHYALEQKKELTQSEQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSIASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWNYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            3562..>3862
                     /locus_tag="ACGE70_002609"
     CDS             3562..>3862
                     /locus_tag="ACGE70_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694203.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVNGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCK"
BASE COUNT         1517 a          553 c          756 g         1036 t
ORIGIN      
        1 tggcctatta cttgttaagt gagccaaaac agatcgcggc gtcaaatatt ttcacggcag
       61 taatgacatt aatgtgtgtc atgtgctttt ctatttttag ttacttactc gtccaattta
      121 tcaggcaaaa tttaacaatg agtaaacttt tcaaaagttt attggactta cttatcttag
      181 gcagtatgtt aacgactcta acagatcggt tcgaacaatc tgaaaaaacg gtagagggga
      241 taaagaactt cttaacgacg attgatcaat ttataaatgg atatatgtat ttgtttgaag
      301 taggctttat tctcactttg tttggttgtg tatttataaa actcattaaa agatcaatcg
      361 aaaaagaaat ggttattgat ctaattccga cgctgagcgg ctatgagtat tacgttgatt
      421 ttggaaagta tgaggagttg atccatacgg atatcgttca cacgagtatc catgaagtaa
      481 ccgttaaggg catcagatct actttaaaag atggacagga gacgaaaaca atcgaccaaa
      541 gaaaagaggt ctacaatttt tataaagttg aagcaagtct acgatatggc attgggaaat
      601 tgaggttaga tgggagtaat attcaacaaa acaaaccata tgttttagga aaagtggtga
      661 tttagaactt cctatgaaag atgaataaat aaggagaatc ttttgaaaat gattcttctt
      721 tacagaattg tgcttatagt tggcacggag ttaaaaatct taaagaagga gcagttattc
      781 aaagtgaaag aacatcataa tcagttacta tgtattaaaa cgggtacgat gaacattcaa
      841 taccgaacag aatatttaga attaacgaat caaactgatt ttttgtcgat tcgagagcaa
      901 cataagcaag aaaaattaat ggctacttat agccaattga ttgatgagtg ggaacaattc
      961 aaggaatcta aaacataaaa ttccatttaa acaccttatt ttttaaagag cataaggaaa
     1021 cgtgacccca cttttaaaaa aagtggttgg ataggctgtg cctatcacgt ttctgaaaaa
     1081 aataaatgac cccaaaatac caatatatag gatagtacaa ggatagcaaa ggatagtaaa
     1141 aggatagagg taggatagcg aaaaggatag gatccttttc actatcctat attatattgg
     1201 aggatattta tgaatgttaa aattagagat ttagatccaa aattcatttc tgaaattgat
     1261 cgaagatgcg ttgaactaag taatcgtaca ggaaataaat ggagtcgtaa tgattattta
     1321 aagctactcg ttgaaaatga ttttgatcgt cctttgatgg aatacaaaca agaaaagttt
     1381 gaccagttat tagaaaagtt ttcagaaatt caaagttata acacaaagat attagaagaa
     1441 tatgttagcc aaaataatcg tattatagaa atcttgattg agcaaaatag aggaagtgaa
     1501 ctttgatatg gcaaaggtta tagacaaaca catttacttt caccaaacac atatcgatgt
     1561 attggatgaa actatttcta ctcatgcaga ggttaaaaac tatagtgaag ctgttcgctt
     1621 tttatgtatg aactatgatc cagaagtgga gaaaaaagta aattccaatc aagcaaagtt
     1681 gaatggaatg agtaaagaaa tttctatttt aaccgaaatg gtttcagaat ttgcggatct
     1741 tcatttgaaa gatacaggaa tattaattgg taaaaattca actgtttatc aagaagcaaa
     1801 agaacgagtg gaaggaaaaa ttaaatccaa tcaaacaaaa aaatattccc ctcagttgaa
     1861 gaaacctgtg aatgaatctg ttcatgccag agtagatcca tttgatccaa cgaagattct
     1921 aaaaagataa aaatgaagga ggataataat gactcaatcg ccttcagtta ttctcacttg
     1981 tcaatttact ttgccgaata aaaagtcatt ttctacttat attgactata ttacaaggga
     2041 acaggctctt gaagaaaagg aacgacgtac gcccgaagaa gaacaggaac ttcaacgaat
     2101 caagaatgct ttagaggagt tttatattcc tgaaggagaa actttttcgg agagtaagac
     2161 gggtaaagaa ttcagtgaaa cgtcgaatga agctcaatcg ttgatggaac atggaatcaa
     2221 ttttgacaag ttagaggaac aagattttac caattatctt tcttatatga cacgtcacta
     2281 tgcgttagaa caaaagaaag aactgacgca aagtgaacaa aaagaatacc aacgcttgaa
     2341 ccaagcaatg aaaaaatatc aaacagaacc ccataaaaaa gcttcaggaa atcttcctgg
     2401 agttttttct atagcttcag acgaagtgaa aggaagtgat ctgaaggaga ttaagcaaat
     2461 cttccaaaga ggacaacaaa aaggctcaat catttatcaa gatgttgttt ctcatgataa
     2521 ttcgtatctt gaaaaaatag gattgtacga tcccaaaaca gatactttag atgaagaagg
     2581 gttaaaagca gctggaagaa aaatgatgga gacattattc gagaaagaac aattgaatga
     2641 aacaggttac tggatggcaa cgattcaccg taacactaaa catatccata ttcattttgc
     2701 agttgttgaa aaagaaaata cgcgtaaacc ttacacggag gttgaaaatg gtgtgagtta
     2761 tatcgtaccg aaaggtaaga gaagacaagc aacattagac gcaatgaaga ccaattatgt
     2821 acatgagtta gaacgttttt cttatgaaaa ggatcgaatg attggtatgg agaaacgaaa
     2881 tttattgacg cggaaaagtg atttgcgtaa caccttaaca aaagtggtaa aagagccagc
     2941 gcgttacgat cgacacgcga tgaacttatt aaatgaagta tataaaaccc tacctgacaa
     3001 acgttcggaa tggaattacg gggatgaaaa acgaaccaaa ctttcaccat acacaagaga
     3061 gaaactcgat gagttaacca aatatgtttt aacaaaagaa cctgaatatc aagaatatat
     3121 acagatttca aaagcattga aagaagaagc aaaaagtctt tatggggaaa gcaagagaga
     3181 atcaaaggat gcagagaaaa atgctctctt tgatttaaaa aagcgcacag gaaatgccat
     3241 cctaacagag ctgaagagac aggatagtca aataaaacca ttaatagaaa gtgttgatca
     3301 gatgcaatct atttacaatg aacgatcctt tcgaacgctt aaacaattgc caacgaatga
     3361 atacaaatat agttacgaag aattcatgaa gcgcaggaaa caattccaac aaagagttgt
     3421 cacgcagcgg agtttgcgtc aattaaatcg aacgattaat gcccatgaag aaaaatacca
     3481 cgcaagtaaa gcatacgaag agcaacaacg aagaattcaa atggaacaag aaagacagta
     3541 aagacagtag gaggaatagc aatgaaacaa tatttagatc aatggaaagt tatagaagga
     3601 tcattaagag aagaacgaat cgaacaactt cctgattgtt tagaaaaaga gcatttgttc
     3661 cagataagag aaatgttgag aaacgaacag tttgatccaa atcaattttt agtcgttgag
     3721 tacccagcta ctggtgtgta ttgttgtaat catgtgaatg gtgaaaaata ttttatcatt
     3781 caagaatatg agggcaagtt agcaccttat tatactactt gggaaatgaa tgaagaggga
     3841 atcaataact ttccatgtaa aa
//
LOCUS       DAXWOO010000194         3776 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.198, whole genome shotgun sequence.
ACCESSION   DAXWOO010000194 DAXWOO010000000
VERSION     DAXWOO010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3776
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.198"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="ACGE70_002610"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="ACGE70_002610"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFF8694204.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="ACGE70_002611"
     CDS             complement(1172..1867)
                     /locus_tag="ACGE70_002611"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFF8694205.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="ACGE70_002612"
     CDS             complement(1880..2596)
                     /locus_tag="ACGE70_002612"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFF8694206.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3531
                     /locus_tag="ACGE70_002613"
     CDS             2926..3531
                     /locus_tag="ACGE70_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFF8694207.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
BASE COUNT         1078 a          774 c          669 g         1255 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcgaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 agcacaggtg aaggtggcga accagaagtg atcggttcaa cagaagaata atactgaaac
     3541 aatcaggtgg tgttagaagc ggcgggtcag cagcttttaa cacgcctttt ttatttctaa
     3601 tcgtttattt tcatatgggg cttccacttt tgtaaatggc tttacaaacg aaaaatccta
     3661 atttaccaag cttttcctcg catactttta tcgtaccttt ataaaaattt atactttgaa
     3721 aaaaagtatt caattattgt tgcatttttc ctgattcttt attagaatga accctc
//
LOCUS       DAXWOO010000195         3763 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.199, whole genome shotgun sequence.
ACCESSION   DAXWOO010000195 DAXWOO010000000
VERSION     DAXWOO010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3763
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.199"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..523
                     /locus_tag="ACGE70_002614"
     CDS             <1..523
                     /locus_tag="ACGE70_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694208.1"
                     /translation="EKAIPYIAAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTIS
                     NEINQYREREPEIQPAEVKQEHKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTL
                     HLKDAAFIEDPQHNQVILKGKSYGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKNE
                     IYYEQQLSQKERV"
     gene            584..2053
                     /locus_tag="ACGE70_002615"
     CDS             584..2053
                     /locus_tag="ACGE70_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFF8694209.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVGDDRLDHAVAFLWKDPQTGETVGASYQGTIVDFNRFGKRGTYKHIDKNPTPNHGFN
                     LKIGDPKHLKFFESSIDLLSYAALNREKLQDAWLVSMDGLKHHVISHYVEESISELRR
                     KQTFPQSIEICVDNDRAGHIFYEKEQMKGIVDPFTNKKIRCERGIPNDWQVPKEYKAT
                     YEAVAKEMSVEPEAIMAIHKTETNLQLTNQLVSAHDVQSTFGKMLAKGEPVETIDLKE
                     ACTTVAKELKVCERADGTYNFDRFYSRKANIKDVNAGILLSYKAEQYYKGYKKHEHEF
                     VPEVKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            2064..>3763
                     /gene="topB"
                     /locus_tag="ACGE70_002616"
     CDS             2064..>3763
                     /gene="topB"
                     /locus_tag="ACGE70_002616"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFF8694210.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     "
BASE COUNT         1392 a          638 c          832 g          901 t
ORIGIN      
        1 ggagaaagct attccttata ttgcagcatg gacgaagaat ggtcagacgt tggatgataa
       61 ggaagctgcg gaacgtggga aaatcatgaa tgatgtcagc cgtgtcgcaa atgaattcat
      121 tcaaaccatt tctaatgaaa tcaatcaata cagagaaaga gaaccagaga tccaaccagc
      181 agaagtgaag caggagcata aaaatactgt agaaacggca gaacaaaaga atattgttcc
      241 tgttggttca ctttggatcg acaagaaaga cgggaaagta atggaagaag atacgggtcg
      301 aacgttacat ttgaaggatg cggcgtttat tgaagatcca cagcataatc aagtaatttt
      361 aaaaggaaaa agttatggaa aagagatcca aacggcgtta ccttcagaag aatttgcgct
      421 aggaaattca ttccgatttt cacttgagac agtgtcagaa gtggcaaaac gaaaaaatga
      481 aatctattat gaacaacaat tgtcacaaaa agaacgtgta taaaacaagt aaaaaagact
      541 atctgaaaaa cggatagtct tttttctttt gggaggtttt aaaatgaccg agaaaaaaat
      601 gagtcttatt gatcgatgta aacagatcga tatagtggat tttgctcgaa acaatggaat
      661 ggctgtagtc aataaaggac gagactatcg cttagaagac catgattcat ttgtttttga
      721 tcgaagaaaa caacgttttt attggaacag tcaaaatatt agtggcgaca ttattgaatt
      781 agctaaactt tttttcatag acaaagagat ccaagaccct aagcaacaat ttaaagcagc
      841 cactgatttt attctaaaga atgaagacaa aacagaacga gtggaaaatc ttcattttga
      901 gacagaaaag tacaaagatc accccgttga ttatcaacca ctcacagaaa aaggaaggaa
      961 ttatctgaag gaagaacgaa aactaccaga gtggctgatt gattatgcag aaaaagaagg
     1021 gttgattgct gaactaaagc cgaaacatga acggcaaaat tttttagttg gagacgaccg
     1081 tttagatcat gcggttgctt ttttatggaa agatccacag acaggagaga cagtaggggc
     1141 aagttatcaa ggaacgatcg tcgattttaa tcgtttcggt aaacggggaa cgtataaaca
     1201 cattgacaag aatcctacgc caaatcacgg atttaattta aaaataggcg atccgaagca
     1261 tttaaaattc tttgaaagta gtattgatct gttaagttac gctgcattga atcgggagaa
     1321 actacaagat gcctggttag tttcgatgga cgggttaaaa catcatgtaa tcagtcatta
     1381 cgttgaagag tcgatttctg aattaagacg aaaacaaaca tttcctcaat cgattgaaat
     1441 atgtgtggac aatgatcgtg caggacatat tttttatgaa aaagaacaga tgaagggaat
     1501 cgttgatcct tttaccaata agaaaatccg ttgtgagcga ggaattccaa atgattggca
     1561 agtacctaaa gaatacaaag ctacttatga agcagtcgca aaagaaatga gtgttgaacc
     1621 tgaagcaatt atggccatac acaaaactga aactaattta cagctgacaa atcaattggt
     1681 gtctgctcat gatgttcaat ctacgtttgg aaagatgttg gcgaagggcg aaccagtaga
     1741 aacaattgat ttgaaagaag catgtaccac ggtcgcgaag gaactgaaag tatgtgaacg
     1801 cgcagatgga acatacaact ttgatcgttt ttatagtaga aaagccaaca taaaagacgt
     1861 taatgcagga atccttcttt cctataaagc agaacaatac tacaaaggct ataaaaagca
     1921 tgaacatgaa tttgtacctg aagtgaaaaa ggattggaat gatcaattga agcatgagat
     1981 ccagcaacaa gaaatcagaa agcaaaaacg tgcgatgttg tttcaacaag gcagacaaca
     2041 agaaagggag tgaagtcgtc actatgaaaa ccgtcatttt agcagaaaaa ccgtcacaag
     2101 cgaaggctta tgccgatagc ttttctaaag ctacccgaaa agatgggtat tttgagatcc
     2161 aagatcgatt gtttacagga gaaactgtga tcacgtatgg ttttggtcat ttagtcgagt
     2221 tagattcgcc cgatatgtac gatgaaaaat ggaaacaatg ggcattagaa catttgccta
     2281 ttttccccaa tcaataccac tatcatgttc ctaaagataa gaaaaagcag tttagtgtcg
     2341 tcaaacgaca gctgcagtca gctgatacga ttgtgatcgc gactgatagc gatcgtgaag
     2401 gggagctgat tgcctggtca atcattcaac aagcaggcgc caatcaagga aaaaccttta
     2461 aaagactttg gatcaactcc ttagaaaaag aagccattta tcagggattc caacagttac
     2521 gagacgcaga agagacctat ccaaaatttg aagaagtaca agcacgtcag atcgccgatt
     2581 ggttaattgg gatgaacggt agcccactgt atagtttact cttgcagcaa aaaggcatac
     2641 caggaagttt ttctttaggt agagtccaaa ccccaacact ctatatgatt tatcaattgc
     2701 aggaaaagat tagaaacttc caaaaagaac catattttga aggaaaagcc caagtaatcg
     2761 ctcaaaatgg cgcatttgat gcgaagcttg atccaaatga aacccaagcg acacaagaag
     2821 catttgaagc ctatttaaaa gaaaaaggtg ttcaattagg aaaacagcca ggtacgatcc
     2881 ttcaagtcga aacagagaaa aaaagtgcgg ctagcccacg tcttttttct ttatcgagcc
     2941 ttcagtctaa aatgaatcaa ctcatgaaag ctagtgccaa agatactttg gaagccatgc
     3001 aagggttgta tgaaggaaaa tatctaagct atccgcgaac ggatacccct tatattacgg
     3061 aaggggaata tgcgtatctg ttggatcact tagacgaata caagcatttt ttaaaagccg
     3121 aagcgattcc gactcctatt catacaccga atagtcggta tgtgaataat aagaaagtcc
     3181 aggaacatta cgcgatcatt cctacaaaaa cggttatgac agcagcggct tttgaacaac
     3241 taagtccact gcagcaggct atttatgaac aagtgttaaa aacaacggtg gccatgtttg
     3301 cggaaaagta tacctatgaa gaaacaacga tcttgacgca agttcagcag cttcagttaa
     3361 aggcaatcga aaaggtgccg ctagatctag gttggaagaa gttatttgga aaagagagcg
     3421 acggaaaaga gaaggaagaa gagccgctac ttcctaaagt gacaaaagga gaaatggtga
     3481 cggtggatct acaagttctt gaaaaagaaa ccaagccacc acaaccttat accgaaggta
     3541 cgttgatcac tgcaatgaaa acagctggga aaaccgtgga tagtgaagaa gcgcaatcca
     3601 tattgaaaga agtggaagga atcggtacag aagcgacacg ggcgaatatt attgaaacct
     3661 tgaaacaaaa ggaatatatc aaggtagaga aaaataagct cgtggtgacg aataaaggga
     3721 ttttgctttg ccaagccgtg gaaaaagagc cgctgcttac gag
//
LOCUS       DAXWOO010000196         3754 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.200, whole genome shotgun sequence.
ACCESSION   DAXWOO010000196 DAXWOO010000000
VERSION     DAXWOO010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.200"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..523
                     /locus_tag="ACGE70_002617"
     CDS             <1..523
                     /locus_tag="ACGE70_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HFF8694211.1"
                     /translation="EKAIPYIAAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTIS
                     NEINQYREREPEIQPAEVKQEHKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRTL
                     HLKDAAFIEDPQHNQVILKGKSYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNE
                     IYYEQQLSQKERV"
     gene            584..2044
                     /locus_tag="ACGE70_002618"
     CDS             584..2044
                     /locus_tag="ACGE70_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFF8694212.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFIPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            2055..>3754
                     /gene="topB"
                     /locus_tag="ACGE70_002619"
     CDS             2055..>3754
                     /gene="topB"
                     /locus_tag="ACGE70_002619"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFF8694213.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAESIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ENDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     "
BASE COUNT         1382 a          648 c          825 g          899 t
ORIGIN      
        1 ggagaaagct attccttata ttgcagcatg gacgaagaat ggtcagacgt tggatgataa
       61 ggaagcggcg gaacgtggga aaatcatgaa tgatgtcagc cgtgtcgcaa atgaattcat
      121 tcaaaccatt tctaatgaaa tcaatcaata cagagaaaga gaaccagaga tccaaccagc
      181 agaagtgaag caggagcata aaaatactgt agaaacggca gagaataaga atattgtgcc
      241 cgttggttca ctttggatcg acaagaaaga cgggaaagta atggaagaag atacgggtcg
      301 aacgttacat ttgaaggatg cggcgtttat tgaagatccg cagcataatc aagtgatttt
      361 aaaaggaaaa agttatggaa aagagattca aacggcgtta ccttcagaag aatttgcgct
      421 aggaaatcca ttccgatttt cacttgaaac agtgtcagaa gtggccaaac gaaaaaatga
      481 aatctattat gaacaacaat tgtcacaaaa agaacgcgta taaaacaagt aaaaaagact
      541 atctgaaaaa cggatagtct tttttctttt gggaggtttg aaaatgaccg aaaaaaaaat
      601 gagtcttatt gatcgatgta aacagatcga tatagtggat tttgctcgaa acaatggaat
      661 ggctgtagtc aataaaggac gagactatcg cttagaagac catgattcat ttgtttttga
      721 tcgaagaaaa caacgttttt attggaacag tcaaaatatt agtggcgaca ttattgaatt
      781 agctaaactt tttttcatag acaaagagat ccaagactct aagcaacaat ttaaagcagc
      841 cactgatttt attctaaaga atgaagacaa aacagaacga gtggaaaatc ttcattttga
      901 aacagaaaag tacaaagatc accccgttga ttatcaacca ctcacagaaa aaggaaggaa
      961 ttatctgaag gaagaacgaa aactaccaga gtggctgatt gattatgcag aaaaagaagg
     1021 gttgattgcc gaactaaagc caaaatatga acggcaaaat ttcttagttc gagacgatcg
     1081 tttagatcat gcggtcgcct ttttatggaa agatccacag acaaaagaga cagtaggggc
     1141 gagttatcaa ggaacgttca ttgattatga acgttttggt gagagaggca catataagca
     1201 tatagataaa aattcgacag cgaatcatgg ctttaaccta aagatcggtg accctaagca
     1261 gttaaaattt tttgagagta gtattgattt acttagctat gctgcactga atcgtgatca
     1321 gttaaacgat acgtggttag tctctatgga agggttaaaa caccatgtga tcagtcatta
     1381 ttttggtgag gcagtatctg aattgagaaa gaaacaagcc tttcctcaat ccatcgagat
     1441 ttgtgtggat aatgaccgtg caggacatat tttctacgaa aaagaacaat taatgggcgc
     1501 agtcgatcca tttacgaatc agaaagtacg ttgtgaacga ggaatcgcta atgattggca
     1561 agtgccaaaa gaatacaaaa tcatttatga agaagtcgca aaagaagaaa aggtcacacc
     1621 agaagctatt atggccatac acaagacaga aaacaatctg caattaacga accaactggt
     1681 ttccgctcac aaagtaaatg cgttttttgg ccagcagctt tctgttaacg attcaataga
     1741 agccattaat ttgaaagata tttgtagaga agtcgcaaaa gaattaaaag tttgtgaacg
     1801 cgtggatggt acgtacgatt ttgatcgttt ttatcaagaa aaaggggata ttaatgcgca
     1861 gattcttttc tcttataaag cagaacaata ctacaaaggc tataaaaatc atgaacatga
     1921 atttatacct gaagtaaaaa aggactggaa cgatcaattg aagcatgaga tccagcaaca
     1981 agaaatcaga aagcaaaaac gtgccatgtt gtttcaacaa ggcagacaac aagaaaggga
     2041 gtgaaatcgt catgatgaaa accgttattt tagcagaaaa gccgtcacaa gcgaaagctt
     2101 atgccgatag cttctctaaa gccactcgaa aagacgggta ttttgaaatc caagatcgat
     2161 tgtttcctgg tgaaactgtg atcacgtatg gttttggtca tttagtcgaa ttagattcgc
     2221 ccgatatgta cgatgaaaat tggaaacaat ggtcattaga acatttgcct attttcccta
     2281 cacattatca ctatcatgtt cctaaagata agaaaaagca attcaaggtg gtgaaacagc
     2341 aacttcaatc ggccgacacc attattattg cgacagatag cgatcgtgaa ggggaactga
     2401 tcgcttggac gatcattcaa caagtaggcg ccgatcaagg aaaaaccttt aaaagacttt
     2461 ggatcaactc cttagaaaaa gaagccattt atcagggatt ccagcagtta cgagacgcaa
     2521 aagagaccta tccaaaattt gaagaagcac aagcacgtca gatcgccgat tggttaattg
     2581 ggatgaacgg tagcccactg tatagtttac tcttgcagca aaaaggcata ccaggaagct
     2641 tttccttagg cagagtccaa accccaacgc tttacatgat ttatcaattg caggaaaaga
     2701 ttagaaattt caagaaagaa ccttattttg aaggaaaatc cgaagtaatc gctcaaaatg
     2761 gcgcatttga tgcgaagctt gatccgaatg aaacccaagc gacgcaagaa gcatttgaag
     2821 attatttaaa agaaaagggg gttcaattgg gaaaacagcc aggcatgatt cgccaagttg
     2881 aaaccgagaa aaaaagcgcg gcaagtccac gtcttttttc tctatccagc cttcagtcta
     2941 aaatgaatca gctcatgaaa gctagtgcca aagacacttt ggaagccatg caaggcttgt
     3001 atgaagggaa atatctaagc tatccacgaa cagacacccc ttatattacg gaaggggagt
     3061 atgcgtattt gttggatcat ttagatgaat acaagcattt tttaaaggcc gaatcaatcc
     3121 caacccctat tcatacgccg aatagtcggt atgtgaataa caagaaagtc caggaacatt
     3181 acgcgatcat tcctacaaaa acggttatga cagcagcggc ttttgaacaa ctaagtccac
     3241 tgcagcaggc catttatgaa caagtgttaa aaacaacggt ggccatgttt gcggaaaaat
     3301 atacctatga agaaacaacg atcttgacgc aagtccagca gcttcagtta aaggcaatcg
     3361 gaaaagtacc gatagatcta ggttggaaga agttatttgg caaagagaac gacggaaaag
     3421 agaaggaaga agagccgcta cttcctaaag tgacaaaagg agaaatggtg acggtagatc
     3481 tacaagtcct tgaaaaagaa accaagccac cacagcctta taccgaaggt acgttgatca
     3541 cggcgatgaa aacagctggg aaaaccgttg atagtgaaga agcgcaatcc atattgaaag
     3601 aagtggaagg aatcggtaca gaagcgacac gggcgaatat tattgaaacc ttgaaacaaa
     3661 aggaatatat caaggtagag aaaaataagc tcgtggtgac gaataaaggg attttgcttt
     3721 gccaagccgt ggaaaaagag ccgctgctta cgag
//
LOCUS       DAXWOO010000197         3751 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.201, whole genome shotgun sequence.
ACCESSION   DAXWOO010000197 DAXWOO010000000
VERSION     DAXWOO010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.201"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            138..2579
                     /locus_tag="ACGE70_002620"
     CDS             138..2579
                     /locus_tag="ACGE70_002620"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFF8694214.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2909..3613)
                     /locus_tag="ACGE70_002621"
     CDS             complement(2909..3613)
                     /locus_tag="ACGE70_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694215.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT         1215 a          655 c          817 g         1064 t
ORIGIN      
        1 cataaaaaga ttaaaagaaa tataaaaaag agaatattta aggattgtta aaatttaatc
       61 ttagaaaaaa ataactggaa caagagctat ttgtttttac agcaaagaat ggtatatttt
      121 tgtaggaggt ggcttacttg aaagaagtcg ttatgataga tgctgcaaga acgccgatag
      181 gaaagtatcg cggaagttta agtccattta ctgcagttga attaggcaca cttgtgacta
      241 aaggattatt agataaaaca aaactgaaaa aagataaaat cgatcaagta atatttggaa
      301 atgtcctgca agctggtaat ggtcaaaatg ttgccagaca gattgctttg aacagcggtc
      361 tccctgtaga tgtcccagca atgacgatca atgaagtttg cggatctggt atgaaagcag
      421 tgatcttagc acgccagctg atccaattag gtgaagcaga acttgtgata gccggcggaa
      481 cagagagtat gtctcaagca cctatgctga aacagtatca gtcagaaaca aatgaatatg
      541 gtgaaccaat ttccagtatg gtcaacgacg gattgactga cgcattttca aatgcacata
      601 tgggattaac cgcagagaat gttgcaacac aattttctgt gagcagagaa gagcaggatc
      661 gctatgcctt gtcgtcccag ttgaaagcag cacatgctgt cgaagccggt gtattttctg
      721 aggagatcat cccagtcaag atttctgatg aagacgtgtt atctgaggat gaagcagttc
      781 gtggaaatag tacattggaa aaactgggca cgttacgtac agtattctca gaagaaggaa
      841 ctgtaacagc aggaaatgct tccccgttga atgacggtgc ctctgtggtg atccttgcat
      901 ccaaagaata cgcagaaaat aataatctgc cttatttagc aaccatcaaa gaagtagcgg
      961 aggtcggtat tgacccaagt atcatgggaa tcgctccaat caaagccatt caaaaattga
     1021 cagatagatc aggaatgaat ttatcaacaa tcgatctttt tgagatcaat gaagcatttg
     1081 cagcttcttc tatcgtagtt agccaagaac tgcagttaga tgaagaaaag gtcaatatat
     1141 atggaggagc aatcgcatta ggccatccaa ttggtgcaag cggtgcacgt atcttgacta
     1201 cattagctta cggattgctt cgtgaacaga agagatacgg tattgcttca ttatgtattg
     1261 gtggaggttt aggcttggca gtcttgttag aagcgaatat ggagcaaaca cataaggatg
     1321 tgcaaaaaaa aaagttttat cagcttacgc catctgaacg gcgcagtcaa ttaatagaaa
     1381 aaaatgtatt gacacaagaa actgcgttga tttttcagga acaaacactt tctgaagagc
     1441 tctccgatca catgatcgaa aaccaagtga gtgaagtgga aatcccaatg gggatcgcgc
     1501 agaattttca aatcaatggc aagaaaaaat ggattccgat ggcaacagag gaaccatctg
     1561 tcattgcagc tgcaagtaat ggtgcaaaaa tctgtggaaa tatctgcgca gaaacacctc
     1621 agcggctgat gcgagggcaa atcgttctat caggaaaatc ggagtatcaa gcagtgatca
     1681 atgcagttaa tcatcgaaaa gaagagctga ttctttgtgc aaatgaaagt tatccttcaa
     1741 tcgtgaaacg cgggggcggt gttcaagata tctctactcg ggaattcatg gggagtttcc
     1801 atgcatactt atctattgat tttttagtgg atgtaaaaga tgcaatgggc gctaacatga
     1861 tcaactcgat tcttgagagt gtggcaaaca agttacggga gtggttccct gaagaagaga
     1921 ttctattcag tattttaagt aattttgcta cagaatcatt ggcttctgcc tgttgtgaaa
     1981 tcccattcga acgcttaggg agaaacaaag aaatcggtga acaaattgcc aaaaaaatcc
     2041 aacaggcagg ggaatatgcg aagcttgacc cttaccgtgc agcaacgcac aacaaaggga
     2101 tcatgaatgg tatagaagct gtagtggcag ctacaggaaa tgatacacgt gcggttagcg
     2161 cttcgatcca cgcctacgcg gcaagaaatg gattatacca aggattaaca gactggcaga
     2221 tcaaagggga taaactagtt ggaaaactaa cggtgccttt ggctgttgcc acagtaggcg
     2281 gagcttccaa tatcctgcca aaagcaaaag catctttagc tatgttagat attgattcag
     2341 ccaaggaact agcacaagta attgctgcag tagggttagc tcaaaacctg gctgctttgc
     2401 gtgcattagt tacagaagga atccaaaaag gccacatggg acttcaagct cgttcattag
     2461 cgatctcaat tggggctatt ggtgaggaaa tcgagcaagt tgcgaaaaaa ttacgtgaag
     2521 cagaaaagat gaaccagcag acagctatac aaatattgga aaaaatccgg gaaaaataaa
     2581 aaagcaatat ctttagaaga ataaagcaag atttacagtt attttattta ctgcagatct
     2641 tgcttttttc tctatcagaa aatatgttta ttattagaat tttttatagt taaggttagg
     2701 catcttaaga aatcaggcaa ctccaagata tcttcaggta gaagagaagt cgcctaacat
     2761 tgtaggatca taggtgattt gttggacagg tcaagctgat atttgagcct gatgttagaa
     2821 ggaatttctt tttacttgct gtagaaatta ttatatgact aaaaaggctg ggattttaaa
     2881 tcccagcctt ttatgacaaa tagaattttt atcgatcagt aatcgctgtc gctgcaaatt
     2941 gttcgatcaa aggaatcaaa gtcaatttga ctttgtattt ccgttcgagt gctttttcga
     3001 taacgaactc attttcagta gtcatagaaa tagttgctaa tttacgaggt tcatcagcat
     3061 ttttttcatt aataactact tcatgttcat tttgttctgt gtaaaattta ggatactcag
     3121 caacggcttt tgaaaaatca gcaaaactta aagcggcatt cgtatcagtt gagcgtgtat
     3181 tttcagttgt gtccgctttt ggtttttccg ttttttcttc tggcacactg actgtttctt
     3241 ctgtaactgg tgtcgcaaca gcttgcgcag ttggttcatc gtttttcaat cgttgatcat
     3301 tccagtaaga cacgatttca ataagaagat tgtcacggaa tcgttcgaaa atcccgtttt
     3361 ctcctgaacg aattctcttt aatacagtat tgaagttgtc atcgattggc acgttgatcc
     3421 gtgtatcttc atttgttttc ccagctaatc cgatcgttaa gatttgacgg tttttgggat
     3481 caagcatgac ttgtaacggc aaaccataac gtcgcaagct ctcttcttct agagaacgga
     3541 ataattgtcc tcttacacca tccaatgaag gacggaattg atttaataat ttttgtttct
     3601 gttttttctt catatgttct ctccttcgct catacccttt cgattataag aaaaaaacag
     3661 ccaaatgaaa aggagtaatg caaaaaaact tgcgtatacg caaattacaa cagaaaaatt
     3721 tgcgtatttt cccgtttttt gaaagaaaaa t
//
LOCUS       DAXWOO010000198         3718 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.202, whole genome shotgun sequence.
ACCESSION   DAXWOO010000198 DAXWOO010000000
VERSION     DAXWOO010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.202"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            43..861
                     /locus_tag="ACGE70_002622"
     CDS             43..861
                     /locus_tag="ACGE70_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFF8694216.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAINENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWIVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
     gene            874..1161
                     /locus_tag="ACGE70_002623"
     CDS             874..1161
                     /locus_tag="ACGE70_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694217.1"
                     /translation="MKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETTSSLVAKLKS
                     DSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(1343..1696)
                     /locus_tag="ACGE70_002624"
     CDS             complement(1343..1696)
                     /locus_tag="ACGE70_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694218.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDRNDSKVAEVKNKPTDHNPIQQAHPSTVTSDHSAKKEK
                     PMLKGFNAPSNKDLL"
     gene            complement(1716..2771)
                     /locus_tag="ACGE70_002625"
     CDS             complement(1716..2771)
                     /locus_tag="ACGE70_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694219.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYQDI
                     LSFSLANLARLEDQSDDVLTVNLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDESLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVVEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            2987..3385
                     /locus_tag="ACGE70_002626"
     CDS             2987..3385
                     /locus_tag="ACGE70_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694220.1"
                     /translation="MRLRRNKNYTTIILEDFTDQELLIVGKTKADFIEKGNASIVKAS
                     KVAVEKYFDSLEESAKEIFNGEKAGSYELDGIKFSLIPTTENIIPKKLYYIFSSEKHT
                     SMKPCIFDWQWLIDAVLDVKCSKYNITLGC"
     gene            3388..3561
                     /locus_tag="ACGE70_002627"
     CDS             3388..3561
                     /locus_tag="ACGE70_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694221.1"
                     /translation="MSMLVRKLPLSVREFLFYLVCLIASGFKLIEHIFIFAYVLAMIA
                     FLSGGWLIIYLLS"
BASE COUNT         1255 a          542 c          673 g         1248 t
ORIGIN      
        1 tgtataatgg agttacaaaa gattaataag ggaggtttaa tcatggaaaa gaagttgtca
       61 atcattactg gaaacattga aaaaggtgga acaggtaaaa caacaacaat ctacaatcta
      121 ggtggatatg ctgtcgaaaa tttaggtatg aaggtgctgt atattgatga agataagtcg
      181 caaaacctat caaaacgatt tcctgatgct ttcagtaaag cgaaaagaga gaatacaatc
      241 aatcatttat atcttcatgg atcagctgaa cctttagcta taaatgagaa catggatatt
      301 ttagtagcag gtgaagattt aaaacaagtt gagatgcaaa tgcgtgatat gcctaacaac
      361 agaaccctac tgtttagatg gatcgttgtt aactatgaac agcttaaaga gaagtatgat
      421 ctaattctga ttgatactca taatgatgca ggtctactag ctcaaaatgc ttgggctgta
      481 tctgatcttg tcgtaggcat ttctgatcca tctaaagatg gttttgaagc tttgcttaag
      541 cttggtaaag atattgatgc actagcaaaa cagttgattg atattaagac tggtgaaaca
      601 ctgatggaaa ctaaatacgc tacgatcggt agcaggatca aacataatac taattcaagt
      661 cgtgagttcc tagaattggt accaacgtta tctaattatc ttggatatgt acaagagaag
      721 gaattgctta atgcaactag cttagaaggt aagaatattg ctgagttcgc taaggataag
      781 aatatctatc gtaagaataa ggatttcttt gaaagcacgt ttacgctata caacaaaata
      841 attgatacgg cacaagaata aggagtgatt gaagtgaaag aaaatagatt taaaaaattc
      901 gctgaagctg aagtagaaga agttcctgaa gtggttgaag cagttgaaga acctaaacaa
      961 acacctaaag aaacaacaag ttctttggta gctaaactaa aaagtgatag ttctgaaaag
     1021 aagaaaaatt atcaattcac gcttcaacca tcacaaagaa agaaactatc taaaatcagc
     1081 gaaaagttag gaattcgatc agattcagct ttacttggtg aaatgataga taacttgtat
     1141 gatgaagttt ttggtgagtg atcagtttaa agctgatcac tttttttatt ggaataagcg
     1201 gttaggtata gcttaactaa atttcgtata cgtgtttatg gatatatacg tatacatgtt
     1261 tatatacatg tatacgtata tgtttatgtt tgtatttgta cataataaaa aggctaggta
     1321 ttgtaacctc gcctttttat ttttaaagta agtctttgtt acttggtgca ttgaaccctt
     1381 ttagcattgg cttttctttc tttgctgaat gatcggatgt aaccgttgaa ggatgcgctt
     1441 gttgaatagg gttatgatca gttggtttat tctttacttc agctactttg ctatcgtttc
     1501 tatcgttgcc tgcttcaata attgtaactt cattgttctt tacacgccga tatagattca
     1561 aagctttttt gatcgtctga gagtatttaa cgcctagtgt ttcatattca gattcaagaa
     1621 actctgctaa aggtaactcg ctaacctttt ctctattgat tataactaca taacgctctg
     1681 attttccctt attcatgttg atctccttcg ctgttttatt gagttaaacc aaacttgtaa
     1741 aagcctttta cattagaaaa ttcactgtct tcaacaaaca aggtatgcgg aattttatct
     1801 tttacaagtt gtttgtcgat aatagaagcc ccaccaccag taaagacaac tttatctaag
     1861 gttgtgaagt ttttgaatgt agctaaaact tctgcaacaa ccatattggt gtatgttttt
     1921 atttctcttt caactacttc tgaaatatca aaactttcat ttccgcttgg tttaaataga
     1981 tatacgcctt tttctttgcc ttctcggata atcttttcaa gttggaaagc attaggactg
     2041 atcggaaggt caagcatatt tacgatacga ttatataaag taaaaatacc ggtatccttt
     2101 tgttcacggt tgttttcgtc aatttcaaaa ttttggacaa catcaaaaag tttcgtgcca
     2161 ccaccaatat caacaaaacc gatcttctga ctgatcaatg attcatcttg tacactacca
     2221 tcatcagcaa ggaggaggtt atatagtgtt ccaatcggct gtggcagtac ccaaacggta
     2281 tctacgttta tattataagc tttaccgtca acttcaactg aatgttgtcg tgtggcttgt
     2341 tgcatcacgt atttgacgat tgtttcggta tagtcggatg aaggcagacc taaaactaga
     2401 ttaacggtta aaacgtcatc agattgatct tctaaacgtg ctaggttagc taaagagaaa
     2461 gataggatat cttggtaggt ttcctgcttg tatctgctga tcccaaaacc tagtgattct
     2521 atcacgtagt catcttttcc tagctcgtgt atatccttcc caaaataata gctttcagta
     2581 gtgtttacta ggctatattc agaaacgttt agtgatcgtt ttaatgagga acctatatta
     2641 gaaaaacgtc ctgaattaat gatatttttg ttcaaaatac gagagggaag cgagagtgga
     2701 gaaacgtgtg tactcatttc ttttactgat ttattcccta agtcaacgga aaacaataat
     2761 ggttctttca tggtatgacc tctttctgtt tgtgtttatg gtatttcggt acgaaaatag
     2821 tttacaccta tttactaata gttgcaactg tttataagaa aaaaatgcaa tcaaaaaagt
     2881 ataatcaatt acaataaagt gtaaataaaa aggtgtaaat aaacacttgc aaatttatct
     2941 tattatgata ttataaaagc ataaattata ttaaggagtg atcctgatgc gattgagaag
     3001 aaataagaat tacactacta ttattttaga agattttact gatcaagaat tacttattgt
     3061 cggtaaaaca aaagcagatt ttatcgaaaa gggaaatgct tcaatagtaa aagcttcaaa
     3121 ggttgctgtc gaaaaatatt ttgattcttt agaagaaagt gctaaggaaa tttttaatgg
     3181 agagaaagct ggtagctatg agttagatgg tattaaattt agcttgatcc caacaactga
     3241 aaatattatt ccgaagaaat tatattatat cttttcatct gaaaaacata cttcaatgaa
     3301 gccttgtatt tttgattggc aatggttgat tgatgctgta ttggatgtaa aatgttcaaa
     3361 atacaatatt actttggggt gttaattatg agtatgctag ttagaaaatt accattatct
     3421 gtaagagaat ttctatttta cttggtctgc ttaatcgcat cagggtttaa gctgatcgaa
     3481 catattttta tctttgctta tgtattagca atgatcgcat ttttatctgg cggttggtta
     3541 attatttatt tgttaagcta agttttagat caaaaataat taataaatta aacgataaaa
     3601 taaccgttaa taaatgtaat gaaaggacaa cgtctaacaa tcagcttgta ataagattat
     3661 aaaataacat aatattaaaa taagtgcttt ttaaaggaca aattaagtgt atcaacat
//
LOCUS       DAXWOO010000199         3717 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.203, whole genome shotgun sequence.
ACCESSION   DAXWOO010000199 DAXWOO010000000
VERSION     DAXWOO010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.203"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..243
                     /locus_tag="ACGE70_002628"
     CDS             <1..243
                     /locus_tag="ACGE70_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HFF8694222.1"
                     /translation="CTSEACDFKLWTTIAKKKLTATQLKEIIQNSRTSQPVKGLKGQK
                     GSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            259..408
                     /locus_tag="ACGE70_002629"
     CDS             259..408
                     /locus_tag="ACGE70_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694223.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            482..1075
                     /locus_tag="ACGE70_002630"
     CDS             482..1075
                     /locus_tag="ACGE70_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFF8694224.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKDAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDQYAIQPHYTNKENFDLGVTETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMDILKSPKEMTLYMLPLY"
     gene            1091..1990
                     /locus_tag="ACGE70_002631"
     CDS             1091..1990
                     /locus_tag="ACGE70_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFF8694225.1"
                     /translation="MTPTQFKEKTRNFAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTANPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAYLENARVPFKLDITSGDSIIPAALMYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERIDEKNLYKSVQGTFKE
                     RNNQSVFKHLYDQEMPQLVKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEKAERKQNFKRSQQMNQEMER"
     gene            1993..2478
                     /gene="ssb"
                     /locus_tag="ACGE70_002632"
     CDS             1993..2478
                     /gene="ssb"
                     /locus_tag="ACGE70_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFF8694226.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFSLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            2844..3104
                     /locus_tag="ACGE70_002633"
     CDS             2844..3104
                     /locus_tag="ACGE70_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694227.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            3545..>3717
                     /locus_tag="ACGE70_002634"
     CDS             3545..>3717
                     /locus_tag="ACGE70_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFF8694228.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGD"
BASE COUNT         1348 a          637 c          724 g         1008 t
ORIGIN      
        1 tgtacgtctg aagcctgtga tttcaaacta tggacaacga ttgccaaaaa gaaacttaca
       61 gcgacacagc tgaaagagat cattcaaaat agccgaacaa gccaaccagt gaaagggtta
      121 aaaggtcaga agggttcgtt tgaagcaacg attgttttaa aagaagactt cactacaggc
      181 tttgaatttt ctgaaaagaa aaaaacgaac ttcaagaaaa gaacacgacg cactacaaaa
      241 taatggaggg ttaatcttat gcgtaataca acacacaaac aacaagaaat cgaaaatcaa
      301 aaagccaaaa ttcgttcaga acaggaaaaa atgcgtgcca gcaaacgaag actgcagcaa
      361 ttgaagaacc agcagcggat tcaaaaagaa atcgaattag atcgataaat tcctttcttg
      421 ttaatataaa actaacatgt tatagttgtg ttagttttat attaacttta ggggaagagt
      481 aatgaaagac aaaatccaat gtatttttga taaacataat ggcaacctat ccttgaagga
      541 tgctagaagt gaaggattat cgcctatgac gttagatcga ttagccgaaa aggataaaat
      601 agaaaaggta gcaccaggtt tctatacctt aaaggatgcg ttcgtggatg aattgtatct
      661 cgctcagagt atctattcca gagggatttt ttctcatgag actgcgctcg atctttttca
      721 agtaggaaca tatatcccta aaaagatcaa tatgatgttt cctaaaggat acaatgtatc
      781 aaaggagaaa ttagatcagt atgccattca accgcattac actaataaag aaaattttga
      841 cctgggagtc acagaaacag aaagctttta cggcaataaa attttagtct atgatttaga
      901 gcgaacgtta tgtgacatgt ggaatccaag atacaaagca agcgtagagg taaaacaaga
      961 agcattaaaa gaatatatga caagtaccag tagaaataca aataagctaa gaagatacat
     1021 ggatatttta aaatcaccaa aagaaatgac tttgtatatg ttgccacttt attgagagga
     1081 gtaaagatga atgacaccga cacaatttaa agagaaaaca agaaatttcg cagcagagaa
     1141 acatttaaac ccacaaattg tccaaagata ttatatgatg gaaaaattat tagaatgtat
     1201 tgttgaatcg ccttatcatg atgattttgt attaaaaggg ggatttctga ttggatcaaa
     1261 atacgggtta gaaaaccgtt caaccgttga tattgatacg acttatcgaa attcgaaact
     1321 aacaaaagaa aagttagaaa ctgttttttc tgaattaaca gctaatccta caaaagatgg
     1381 aattcgtttt gaattaaagg gattaacaga gacgagggaa gccgattatt atccaggttt
     1441 tcaagcaaag tttattgctt atcttgaaaa tgcacgtgta ccttttaaat tagatattac
     1501 ttcaggtgat tctattattc cagcagcatt gatgtatgaa cacaaattaa tgtttgaaga
     1561 taaaacgatt catattccag cttatcctac ggaacaaatt ctagctgaaa aattacatgc
     1621 cacgtttagt tttggaaaag ataattcgcg catgaaagat ttttatgacc tgtatatgat
     1681 tccgaaatta gaacgtatcg atgagaaaaa tttgtataag agtgttcaag gaacattcaa
     1741 ggaaagaaat aatcaatcgg tgtttaagca tttgtatgat caggaaatgc ctcaattggt
     1801 gaaagatgag ggcttgagag aaaagtggaa aagataccaa caagaaaact attttgccaa
     1861 agacatttct ttcgaatcta cactaacttc cgttgattcc ctgatgcaat caattgtaga
     1921 gcaagagaag gcagaacgaa aacaaaattt taagagatca caacaaatga atcaagaaat
     1981 ggagagataa agatgatcaa taatgtaact ttagtagggc gattaactaa agatccagat
     2041 ttacgttata ccgcaagtgg cacagcagtg gcgacttttt ccctagccgt taatcgtaat
     2101 ttcacgaatc aaaacggcaa tcgagaagct gattttatca attgcgtgat ttggcgcaaa
     2161 ccagcagaaa cgatggcgac attagctaaa aaaggcagct tgatcggcgt tgtcggacga
     2221 attcaaacac ggacttatga caaccagcaa ggacaacgtg tgtatgtgac agaagttgtg
     2281 gccgataact tccaactatt agaaagtaaa gcagcgacag aaagccgtat gcacgctgat
     2341 caatcgtcca gcagtccaag ccctaccacc tttgaacaac gggatacagc gacgcctaac
     2401 aacaatggat tgaacgcttc acagaatcca tttggtggac aatcaatcga tattagcgat
     2461 gatgatctac cgttctaagt agcattttct attggttgca tttgctacgg tttatgttat
     2521 aatttagaca acaaaaagaa ttttcaatct ataaccaaaa accactagct ggcgggctag
     2581 tggctttctt ttacgtttct attataagaa ttaacgggta ggcttactgt ctttcttggc
     2641 aaatttacaa tcaagccatt tgcagatata gtgcgcggct acttctgcca tgattgcaaa
     2701 aacaaaatca aaaagaattt tcaaacctat aacctcctcc ccttgccaga ttaggccggg
     2761 gtagcagaca gcaagactat tatagcataa ttgaaatatc caaatgattg gatttttgct
     2821 ataattttag cgaggtgatt gtgatgaaca aatatgttgg tttgcttgat aaaatccgag
     2881 taataaaaac gcaaccgtta ttggttcgct ttactttaca aacgattcat gaatcgatta
     2941 attgtgtagt tgcagatatt gaaattatcg ataaattgct aattatggat gatggaaaat
     3001 acaatattgc cgtcacaggt cacttcaata aacgaaatca gctagttatt gctagcatgt
     3061 atgttagaaa tcccgatcat tttacgcgat cgatgggcat ataaataaaa gaagagttgt
     3121 agtcctaaat gaggattgca gctctttttg ttttacatag aaacaggaaa agaccttttt
     3181 aggaagacac aagtgccttc tcaattgaaa aacctttctc tcactccaac cgccaccgcc
     3241 gttcgagaag aaaggaagtg aacgctgcac gttcactaag atatggtgtc ttgcaaagca
     3301 agacaaaata gacaaaactt cctctcacca aaaggttcgt tcctgttcgt tatctcttta
     3361 ttgatttgcc agaccggcaa caagttgcct tttatcacca aaaagcgaca aggacaaggg
     3421 taaatggcac gctgcgcgcc cttgtccttg tcgcttttcg ttgatttatt ctggccaaca
     3481 aatcaataaa gaaagaacta acgaaaggaa gtaaaacctt tcctaagagg aggcagaatc
     3541 aaacatgcga aagaaatttg attattgggg tgttcccttc agcgaattat tcccaaacta
     3601 tcatgcacca catactgttg aatgcgattg tggagaaaga gcgaaatgta tcaaaagcta
     3661 tcgtttgtat caatgtccaa catgcgggaa aaaatatacc ttgtcttatg gcgatta
//
LOCUS       DAXWOO010000200         3663 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.204, whole genome shotgun sequence.
ACCESSION   DAXWOO010000200 DAXWOO010000000
VERSION     DAXWOO010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.204"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..276
                     /locus_tag="ACGE70_002635"
     CDS             <1..276
                     /locus_tag="ACGE70_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HFF8694229.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            419..640
                     /locus_tag="ACGE70_002636"
     CDS             419..640
                     /locus_tag="ACGE70_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8694230.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            656..1498
                     /locus_tag="ACGE70_002637"
     CDS             656..1498
                     /locus_tag="ACGE70_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694231.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1561..3363
                     /locus_tag="ACGE70_002638"
     CDS             1561..3363
                     /locus_tag="ACGE70_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HFF8694232.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYYWKYKD"
     gene            complement(3422..>3663)
                     /locus_tag="ACGE70_002639"
     CDS             complement(3422..>3663)
                     /locus_tag="ACGE70_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFF8694233.1"
                     /translation="PPRKNMDKSEKLDHSLLGKQRKTVGTVFSSLEKLGCQNFNSRSV
                     KGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         1306 a          519 c          746 g         1092 t
ORIGIN      
        1 gaactcatca agctggctaa taagggtcaa gattatgaac atctagcaga tgagattgat
       61 gagctgagag acaagcgaca gctcctttta gtagaagatg cctccctcag tggcgagaac
      121 gagcgaatca atgagctgat tgaatttatc cgcaagaaca aattccgtac cttagagtac
      181 gatgataagc ttgtaaggaa gataatccag agcgttacag tctatgaaga acacttcgtc
      241 atatccttca aatctggcat cgaaatggaa atatgaaaac cagaagtaaa tagcccatga
      301 ttctacagta gagtcgtggg ttttcttttg cttgctgatt ataataatat tttaataact
      361 attgttatta taaaccaaat gatttataat attcttagcg taaacttgga ggtgctgtat
      421 gactacggca gaaatgatta aagaactgtg tgagcaaatg aatataagtg tttccgaact
      481 tgctagacgt attggacaga ctccacagaa tttcaataag aaattacaac gagaaacggt
      541 aaccttagat gagttgaaag cgattgctga tgtgctaggt gtcaagtttg tgcaggcttt
      601 tatattgcct gatggcaatg aaataaagat aggtaattag gaggtgtaat ttgtattgaa
      661 aatcataaca ataaatgatg ttgagtatgc tgtatttgct gctaatgaag gtacttccaa
      721 accacaacct catatcatag aaacgaaatc tggtactatt ccagaaggaa aacaactgag
      781 tttattaaaa gaatatctta agcagaatga tatttcccca ataaaaggag caacaacata
      841 ttggtgcatt gacaaggttc ttaagcttga ttcaagtaaa gaaaaaacta taagtgaaac
      901 aatccataaa caaaaatact tatctttaac tgaagaaaat attgagaaac agcataagtt
      961 tgtaggtgca agctctaatt atgggaaaga aggtctaata atacatgatg ttttgaatgc
     1021 atttcctctt cataatgatt taaatactat tgcgatgaag attgcggtaa ttgatgtaac
     1081 aaattcaaca catctttctc aatataagag caggttgtct ctatatgatt tggctaaagt
     1141 aattctggag attccaaatt ttgatgatcg gttggcaaaa ggagatcctc agttgataaa
     1201 tattatcgca aggaacattg gtgcagttaa tatgttctca tttgcatcca agtactgtac
     1261 atatcataat gtggaagtgt gtgggagaga tgattactcc atctttgatg gtatcgttaa
     1321 aaatactctt ccacactata tccaaggact aactacgaat aagattgata cgtggcgaag
     1381 aagttttgat tacgaggcat ttaacgaatg tgttggaaaa ctacttgatg aaaataatat
     1441 acatattcct tttagaagaa gaaagttaga tcacttcttg tggtatgcga ataggtagaa
     1501 tataagaaaa aggcatcgta aaagtgattc tggataaaat taatggggga ggaaataata
     1561 gtgggattat ttgattttct aaagaaaaaa caaggaaagg ttagtaaaca cgttgattat
     1621 caactggctt ctagtctatt tcgtactggt aaagtttttg ctgaaaaatt ggaagtggac
     1681 atatcaaatg tagatgagtt tcgtaaaact tttatcgctt ttgatgtgga aacaactggc
     1741 ctaaattctt cttcagatag aattgtagag attggtgcgg tgctatttaa agaaggagtt
     1801 ccaattcaat cattcggaac tcttgtcaat ccaaaagtta gaattgcagc ctcagcttcg
     1861 gcaataaata atataactaa tgaaatgcta aactctgctc caaatgagca ggaagtttac
     1921 ccccagttgg tagaattttt aggagatgca ctagatggaa aaatcactat gtgtgcacac
     1981 aatgcaagtt tcgattttgg atttttaagt aacacatttt ctagacttgg gtatgatgct
     2041 aaaattaagt atgttgatac tctgagtctc tctagaaaac atataaaagg cattgtaaat
     2101 cataagcaag agactgtagc ggaatatttc ggattaataa atgacactgc ccatagagcg
     2161 gaatcagatg cagatatatg cggaaagatt ttttggggtg ttttagatat tattgaggag
     2221 tcgttggaag aacaaaaaaa gcaaatagaa aagatgactc cagacataaa agaacttgaa
     2281 gcttgtgctt ttattcaaga tattattgag aaaaaaggat gcgacaccag ttggatcagg
     2341 tatcgaaaaa acagtaataa ttatgtcgat gtaacttgtc tatatacatt cttaaagttt
     2401 aagtttgcta agaagggaaa ttacatcatt attagaaaag atgctacaaa tggaattgat
     2461 ttacctatag aaccttgtac tgtaagtgaa ggcggtacaa caaatgtgcg agtctatttt
     2521 cgtagcccct ttgacttaac gcctctttca aattatattt tcagtgctta tagcgattgt
     2581 tataattcta tgaatgaata tatgagtgtt agtaattaca ctaaaaaaga agcagagcaa
     2641 agccttagta tgctgaaatc tatttcaaaa tcagcgatga aagagttatt attcgatgca
     2701 aaaaatcgtg gatatgatga gactgaagtt aacctaaata ttgaaaaagt gatatctaga
     2761 gaagatgtaa ttattaacgg gttgcataat cgagtttcat taaacaagat acgaaatata
     2821 gggaattggg agaaaggatt tgatgcaggt tatccatatt gggaacgtgg agaaactgct
     2881 aggaaaaatg gcaaagtaga tgaagctatt gcactttttg ataaagctcg atataacggc
     2941 tatgatgctc ctgcgctata tgattcttat gctaaagctt atcgaaaaat taaggattac
     3001 gataatgaaa ttctcattct tgatgaagga attatgcgta aaacacggca cgatgttggt
     3061 actttagagg ctcgtaggga taaggcaatt aagctattat ttgctaaaca agaggcagag
     3121 cggatagcta aggaaaaatc tgattttcta aaagcgaaca aaaaagagga tgcgattagt
     3181 gagcctagca agaagcgtgg aagacagatt attcaaatga ctgatgatgg aacaataata
     3241 aaagaattcg atactatagc ttctgctgta aaagaagttg gcgttaactc aaaaagtatt
     3301 agggatgctg caaatggagt tcaaaaacat gctggaggat actactggaa atacaaagat
     3361 tagcagataa tctaagataa cctaggatct tcttaataga tacccgtggt gctagttaat
     3421 tttaatatcc taaagaatat ctcaaagttc cttcaaaacg tcgttgtgct cgacttaata
     3481 gaacactgta agccagtaat atgctttcaa atctactctc tagcccttta acagaacgtg
     3541 agttgaaatt ttgacaacct aatttttcta aggaagaaaa aacagtccca actgtttttc
     3601 tttgttttcc aagtaagctg tggtctagtt tttctgattt atccatgttt ttacgtggcg
     3661 gaa
//
LOCUS       DAXWOO010000201         3622 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.205, whole genome shotgun sequence.
ACCESSION   DAXWOO010000201 DAXWOO010000000
VERSION     DAXWOO010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3622
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.205"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(45..707)
                     /locus_tag="ACGE70_002640"
     CDS             complement(45..707)
                     /locus_tag="ACGE70_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFF8694234.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            891..2537
                     /locus_tag="ACGE70_002641"
     CDS             891..2537
                     /locus_tag="ACGE70_002641"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFF8694235.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            2528..3610
                     /gene="uxuA"
                     /locus_tag="ACGE70_002642"
     CDS             2528..3610
                     /gene="uxuA"
                     /locus_tag="ACGE70_002642"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFF8694236.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
BASE COUNT         1170 a          647 c          738 g         1067 t
ORIGIN      
        1 aaacgaaggt taattataaa aatagaagag gtcgcaagaa aatgctactg ctggaaatac
       61 tcgggataac gttgagtaac agtttcctgt tctttcagca tcatgtgtaa gtgcaatccc
      121 gtttgaaatc gagcttcttc tggtttttta tcaactaaag ccttgagtaa atcataatgt
      181 tgttccatga tcgtttccca tggaagcccc tgaatggtca ctcgtaacca gcggaaacgt
      241 tctaaatggg tattatggtc atctaaccag ttccaaactt cttttcttcc tgcaatttcg
      301 aaacaagctt catgaaataa attatctgca cggaaaaaag cgcgttcgtc atttattttg
      361 actgcttttt cttgttcttc taaaatcgtt tctagcacta ttcgtgtttg tgtgtccaat
      421 ttggaacaac attcaatcat gatttgctct tccaactgtt ctcggacaaa gcgagcattt
      481 tctgcagaaa caagattaat ttttgaaaca tacgtaccac tttgaggtat cgtatacacg
      541 agttcttgct gacgtagttg aatcaatgct tcgcgaatgg gagtccggcc gatagcaaga
      601 tgttcctcca gtgccttttc agatattttc cttccaggtt ctaagtcagc atagataatt
      661 tgtttacgaa tcgcttgata agcttgagat tgcaagctgc ttttcatctt tattcctcca
      721 aaatcatttc tatgaacgta gtataacaga agaggtctga aaaacaatct ggtatgtcac
      781 attaactagt aaatttgtat aaatagtaaa attttggttg acatagcgat ttttattgca
      841 taaaattcac ttgaatacaa aaactaatat gttagtttag gaggggaaaa atgattgatt
      901 taaacaactg gaggaacttt atccctgaat tatctcaaaa agaagtagaa atacctcatt
      961 ttgaccgtgc atcattaaga gaaaatggac aaaaaaatcc tgtctggatc catttcggtg
     1021 gcggaaatct ttatcgagga tttcatgcag aaatcgctca acgtttagcg gatctaggcg
     1081 aattgtccac tggtgtcatt gtttgtgaaa cattcgatga agaaataatt gaaagggctt
     1141 accaagattt tgatgatttg attcttgaag tggtcatgcc aagcagtgga gagttcgtca
     1201 aacgtatatt agctgcgaca gctgacagtc tgtattgtca tccagattgt tctgaacact
     1261 ttgataagat gattcgacac tttgaaaatc ctagtttaca attagttact tttgctatta
     1321 ctgaaaaagg ttatcgattg aaagatacaa ataataaata tataggaccg attgcagtcg
     1381 atataaaaaa tggtccaaag aagccatcac atacaatgag tattcttgct gcgctacttt
     1441 ataaacgttt tttatctgga gcttggccga ttgcacttgt ctctacagat aatttttcac
     1501 aaaacggtag tcaattacgt acttctttgc taacgatcgc aaaagaatgg caaaaggaag
     1561 gttatgtaca aaatacattt atccaatact tatcgaatga agaaattgtt gcgtttccat
     1621 ggacgatgat tgatcgtatc accccgaatc cttccttgtc agttagcaaa atgttggcaa
     1681 aagagggctt ttcaaatatg tcgattttcc aaaccgcaaa aggaaataat attgctccat
     1741 ttgtcaatac agaggacatc cattatttaa tcatggaaga tcattttccc aatggacgtc
     1801 cgaaattcga aaaagctgga gtattcttga ctgatcgtgc aacggtagat aaagcagata
     1861 cgatgaaggt gacgacttgc cttaatcctt tgcatacgtc tttagctatt atgggttgtt
     1921 tactggggta taggagcata gctgaggaga tgaaagatcc agatttatcg ctgctagttt
     1981 ctgaaatcgg gtacaatgaa ggcctcccag ttgtggagtc tccaggtatc attgatccaa
     2041 agatctttct ggaagaagta attactgtgc gcttttctaa tccaaatatt cctgatatgc
     2101 ctcagcgaat cgccacggat acctctcaaa aggtggcgat tcgttatggt gagacgatta
     2161 aaaaatatat ggaaatagaa gttaaagacc cgcagacact tgtattcata ccattgactc
     2221 tcgctgcttg gcttcgatac cttattggca ttgatgatca aggaaaacca ttcatcccga
     2281 gtccagaccc actattgaca gactttcagc ataagctttc cgatattcaa ctgggtacgc
     2341 aagatacatc acatattcat gagaaaatgg ctcctatttt gtcaaataaa gaaatttttg
     2401 gtgtagatct ttaccaagca gagcttggca gaaaaataga agaaatgttc atcaagatgg
     2461 tagcaggacc gaatgccgta cgaaaaacat tacaggatta cttacaagaa catggaggaa
     2521 atttatcatg gaaatgactt ttagatggta cggatcacag gaagaaaaga ttaccttaga
     2581 tgaaatcaga cagattcctg cggtcaaagg gattgtaggc acattgatgg atataccagt
     2641 aggagaggta tggcctaggg agaggatcaa agaactaaaa gaagaaattg agtctcgtgg
     2701 gttaacacta aaagtaattg aaagcgtcaa tgtgcatgat gccattaaaa ttggattacc
     2761 ttcccgtgag cagtatatcg aaaactataa gcagacaatc cgaaacttgg cagaatatgg
     2821 aatagaagtc atctgctata attttatgcc tgtatttgac tgggtgaaat ctgaccttga
     2881 ctaccgctta gaagacggct catccacgtt agcttttata tctgatgata ttccagatga
     2941 tccaagagaa atcgtcgcgc gcatcgaaca ttccagcaat gaatttgaat tgccgggatg
     3001 ggaacctgag cgcttagctc atatcaaatc cttgcttgaa gcctatgcat cagtagacga
     3061 agaaaaactt cgagaaaatt ttgcctattt tctccaatca atcatcccaa catgtgaaga
     3121 ggtaggggtc aaaatggctg tacatccaga tgatccgcca tactcgattt ttggattacc
     3181 tcgaatcgta aagaatcgag aagatttaga ttggttgtgc cgcatagtag acagccctta
     3241 taacggaatc actctttgta cagggagtat tgcagaagat ccagaaaatg atgtatatgg
     3301 gattttagca gaattttgtc aaagagatcg aatcccattt gcacatgttc gaaatattaa
     3361 atttttagct aatcgagatt tctatgaggc accgcataaa tccgaatacg gttcattaga
     3421 catgtaccgc atactaaaaa caatgtatga caacggattt aacggataca tccgtcctga
     3481 tcatggaaga atgatctgga atgagacagg aagaccagga tacggcttat atgatcgagc
     3541 attaggtgct gtttatctaa atggattatg ggaagcaatc gaaaaaggaa acaaaagtaa
     3601 agataattag tgaaaaaaac tg
//
LOCUS       DAXWOO010000202         3598 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.206, whole genome shotgun sequence.
ACCESSION   DAXWOO010000202 DAXWOO010000000
VERSION     DAXWOO010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.206"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(<1..463)
                     /locus_tag="ACGE70_002643"
     CDS             complement(<1..463)
                     /locus_tag="ACGE70_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFF8694237.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHNKATFEKNYKAYVELARDSAKKYGIPLTLDTPYNQP"
     gene            complement(474..671)
                     /locus_tag="ACGE70_002644"
     CDS             complement(474..671)
                     /locus_tag="ACGE70_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFF8694238.1"
                     /translation="MVLPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            complement(687..929)
                     /locus_tag="ACGE70_002645"
     CDS             complement(687..929)
                     /locus_tag="ACGE70_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HFF8694239.1"
                     /translation="MKDEAIQDVVERLVRIETKLDNYELLREKAESAKDRADQAYSIA
                     LNNAEDIKEMKANNKWSWGYMIGLGITIIGYFLTKL"
     gene            complement(1007..3229)
                     /locus_tag="ACGE70_002646"
     CDS             complement(1007..3229)
                     /locus_tag="ACGE70_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694240.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVIFSLDGTFMSEAGYYKHCYVEYRKGNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGDYDYSGNPNLLSKLSYDLIE
                     NQNTSAGTLSKGENSFKYNKISAEVEGGVELYYKRRGIANWLPSNKTLVMTVKLRAGA
                     DYSPVDGKLILIRYRYVASGTGKIVLDLPINSNSITQEWKEFSITGTTPTFSPQAYHP
                     WIQFRAQDGVLGEIEMSYDIKIEEGSTATPYQPNLLDAPHYLSKTPLGENIANKSVTF
                     PINTSAYKLYEYNTEEDLIIGQTYTITLKGTKPASQTFAVYNYWNINLGSLKPVEGLT
                     DVWSITFTPTTLEPGLPKDLRILQSPKETAGACQIDWLKIEKGDTRTPNIEQYKYRGI
                     GMRDSNNPKDYVWDLAPEYVENNLATDIKVAEITGKANNYTDGKVSQINSQLTASINE
                     VDTTAKGAQTKANANATAIDELDNKIDERINDTATTAFYGAQGEVSNKTKVAEYGVGT
                     GYTTTAVGETHFERQSNGYVKCLKAGKYSISAQIRVQLGRKYASWLYTDLYKDGTSVD
                     NLVSYGVDALQNRFAASGNIVTDLEVNDVLNMRTEIGVADGILQFTSCRTLVLTRLSD
                     "
     gene            complement(3253..>3598)
                     /locus_tag="ACGE70_002647"
     CDS             complement(3253..>3598)
                     /locus_tag="ACGE70_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694241.1"
                     /translation="EHYGQRLLNAYETPEYYFADYGEAVTGDDGKVRVDIDPMFAETV
                     NLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRLGYEDIRLKEDT
                     AYDSTVLDQKRF"
BASE COUNT          993 a          765 c          590 g         1250 t
ORIGIN      
        1 ctggttggtt ataaggcgtg tccaatgtaa gcggaatacc gtatttctta gcggaatctc
       61 tcgccaattc aacgtatgct ttatagttct tttcaaaagt tgctttatta tgtgtgtgtt
      121 gtaactcaat ctgcacagga ctgttggcat tagcatacga accagcaccg tactgtacat
      181 aaccaggttg tccaacttgg taaacaattc cgccatcacc cacaatgtaa gcagtgtaag
      241 cactagtcca tgaacgttgc atatactgcg cttcattgcg tcctgttgct gtttcattag
      301 ccgtttcatg cagtaaaatg tactgattat ttgctacttg tgagctacct tcatttgcgc
      361 ccaaattaaa ttcattgttg atagtatagg caaacccatt aattggcaat aaaaaaagag
      421 ccgttaatag gctcatcgca gtaatagtaa ttttcttttt catttgtttc ctcctatttt
      481 ttcaaattat aagccgacac accagtgata acacctaaaa atgttgctac tgcattgata
      541 gtgagtactg tcatatctgt tccattccat ccatacgctt tccctaacgt cgctactaat
      601 acagatgcag ctggcaatac tgttaaaact gtccatttaa tgacttgata atacttatct
      661 ggtaaaacca tttcttctca cctcctttac aatttagtca agaaatagcc aatgattgta
      721 atgcctaaac cgatcatgta accccacgac catttattat tggctttcat ctctttgatg
      781 tcttctgcat tattaagcgc aatagaatat gcctgatccg ctctatcttt tgcactttcc
      841 gctttttcac gtaataattc gtaattatcc agtttcgttt caatacgcac taagcgttct
      901 accacgtctt gtattgcttc gtctttcaac caactagcct cctttcattg caaaataaaa
      961 aacaccctcc tttgagcgtg ttgatagcaa aattataaat attgtattaa tcagatagtc
     1021 tagtcaagac taaagtacgg caagatgtaa attgtaaaat tccatctgca acgccgattt
     1081 cagtacgcat atttagtaca tcgtttactt ctaaatcagt tacaatgttt ccgctagctg
     1141 caaatctatt ttgcaaggcg tcgaccccat aagatactaa attatcaaca gaagttccat
     1201 ctttatataa atcagtatat aaccatgaag catatttcct gcccaattgc acacgtattt
     1261 gcgcactaat tgaatactta ccagctttta agcatttaac gtatccgtta gattgacgtt
     1321 cgaaatgtgt ttctcctaca gctgtagtag tataccctgt tcctactcca tactccgcta
     1381 ctttcgtttt gttagagact tcaccttgtg cgccataaaa tgcggtagta gctgtatcat
     1441 taatgcgttc atcgatctta ttgtctaatt catctatagc agtcgcatta gcattcgctt
     1501 ttgtttgagc acccttagct gtggtgtcta cttcattaat tgaagcagtc aactgcgaat
     1561 taatctgcga tactttccca tcggtataat tgtttgcttt accagtaatt tcagcaactt
     1621 tgatatctgt agccagattg ttttctacat attctggtgc taaatcccag acatagtctt
     1681 ttggattatt tgagtctcgc atgccgattc ctcggtattt atattgctca atattcgggg
     1741 ttcgtgtgtc gcctttttca atcttgagcc agtcaatttg gcatgcgccg gccgtttctt
     1801 taggcgactg aagaatgcga aggtctttag gcaaacctgg ctcaagtgtc gttggtgtga
     1861 atgttataga ccatacgtct gtcaatccct caactggttt taagcttcca agattaatgt
     1921 tccaataatt atacactgca aaggtttgac ttgcaggttt tgttccttta agcgtaatag
     1981 tatacgtttg acctattata agatcttctt ccgtattata ttcatatagt ttatacgcgc
     2041 tagtgttaat tgggaaagtc acagacttat tagcaatatt ttcacctaat ggtgttttac
     2101 ttaaataatg tggagcgtcc agcaaattag gctggtatgg tgtagctgtt gagccttctt
     2161 cgattttgat gtcatagctc atttctattt ccccaagtac cccatcttga gccctaaatt
     2221 gtatccaagg atggtatgct tgtgggctaa atgttggagt agttccagta atactaaact
     2281 ctttccattc ctgagttatc gaattactgt taataggtaa gtctaaaaca atcttgccag
     2341 ttccactggc aacataccta tatctaatca gtataagttt tccgtcaact ggactatagt
     2401 ccgctccagc tctaagctta acagtcatta caagcgtttt attagagggt aaccagttag
     2461 ctatacctct tcgtttataa tataactcta ctccaccttc cacttcagct gatatcttat
     2521 tatatttaaa cgagttttca cccttagaaa gtgttccagc tgaagtattt tgattttcaa
     2581 ttaagtcgta tgatagctta cttaacaaat tcgggttccc cgaataatca tagtcaccga
     2641 agtcgatgct gttgctgtac atcacttgta agttacctaa cttagaaatt tcttctttca
     2701 gagcatctaa cttgtcttgt agcgttttag cttgaccagt taaatcagta atctgttgat
     2761 ttaagctatc cactctacct ttaatttcag ccataaaagc atcaaaagtt tcgttatact
     2821 ttcgaatcaa ctcttccaat tgcgaaacgt attcatcggc ttggccttgc gaaatgtcag
     2881 acactcctag tgagaaaaaa atgatgtctt gcgttgttaa aatttgatta ccttttctat
     2941 attctacgta gcagtgttta taatatcctg cttcactcat aaatgtgcca tcaagagaaa
     3001 atatgacttc ttcactagtt acgctagttg caacactatc tacataacgg ttagatggtg
     3061 ttgttccttt taaagtaaat gttccgccac tcgtatccat ctgcaagcca tttaaaaatg
     3121 gtttaacagt caccgtaatc cctttatcac cttgacgagc cataatagct ttggtgtagt
     3181 ttaattcttt gctgaaatct aaagccaaat tatataaact gctagccatt tatatacctc
     3241 cttgtcttcg ttttaaaaac gtttttggtc aagcactgtg ctatcatatg ctgtatcctc
     3301 ttttaatcta atatcttcat accctagacg gtgtgccact aaattccatc taactaatac
     3361 gtttggctta ctagtttcaa tgatgaaatg gtcaatatct tcatgagtaa cagcacacaa
     3421 aactagttct gtaggtgtca catgtgtcat ataccgactt agatttactg tctcagcaaa
     3481 catggggtca atatcaacac gaactttacc atcgtcacct gtaacggctt ccccataatc
     3541 agcgaaataa tattctggag tttcataagc gttcaatagt cgttgtccat aatgttct
//
LOCUS       DAXWOO010000203         3551 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.207, whole genome shotgun sequence.
ACCESSION   DAXWOO010000203 DAXWOO010000000
VERSION     DAXWOO010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.207"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            11..736
                     /locus_tag="ACGE70_002648"
     CDS             11..736
                     /locus_tag="ACGE70_002648"
                     /inference="COORDINATES: protein motif:HMM:NF020916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTC domain-containing protein"
                     /protein_id="HFF8694242.1"
                     /translation="MNKKRFKRIERKFALTYDQAKYLKTLFPKQVVRDKYSDHFISSL
                     YFDTNDHHFLKEITTEKSYREKFRIRKYGDPQDISPIYLEIKRKFQGMTDKRRIKLAH
                     HELDDMLENIELAETTFSNLEEEYIFNEIQWLFRRLALEPKVYIYYLREAFVNRDSED
                     LRITFDQNIHYRLMNLTTWEHCTEDNLVAPEIDVLMEVKTTGEIPEWLQVGLDRMGKK
                     EIKFSKYKQVYKRYIQEDPLYVQ"
     gene            726..1400
                     /locus_tag="ACGE70_002649"
     CDS             726..1400
                     /locus_tag="ACGE70_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HFF8694243.1"
                     /translation="MFNSVLKNEPSQLTLDDFFLCMVVSILLGILIAYTHMYQNNCTK
                     NFMFALIGLPTLVHTVILLINGNLGTGIAVLGAFNLVRFRSAQGNSRDIFSIFWATSI
                     GLATGTGYIVYALFVSSIVAAIQVIIFTLPIKNKHTVFNTEKTIKLKVSKSLFLSSII
                     EEKIAEYTENCNLKKVKINKETCSLHYIVKVKTSMLEKELVRTIMDHPEVISITVGMD
                     KSNKLL"
     gene            complement(1688..1972)
                     /locus_tag="ACGE70_002650"
     CDS             complement(1688..1972)
                     /locus_tag="ACGE70_002650"
                     /inference="COORDINATES: protein motif:HMM:NF024076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 family protein"
                     /protein_id="HFF8694244.1"
                     /translation="MRQKCFLLLSHSQKVKPIFWLSIWDGFFKVSFHFPEKTREKLVT
                     LSLSEGTKEKIKTLQANGNKRKHLSVIFDVDHLEQLADIYQLAEFKKQNG"
     gene            complement(2266..3549)
                     /locus_tag="ACGE70_002651"
     CDS             complement(2266..3549)
                     /locus_tag="ACGE70_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFF8694245.1"
                     /translation="MVLLASLILCITSFLPSATVQADDAFKINAKAAFAVDAESGKIL
                     YDQDGEKTMGIASITKIIGLYIVLEQIKEGKLSWEDKVAISDYAEKLSVVPDLSNVPL
                     HKENDYTVKELVDSAFIQSANASMVALAEKVAGSESKFLELMKKQLKDWGIKDATIVN
                     ASGLNNSYLGENRPENTGETDENQLSAKDVAIVARHLILDYPEVLKITSTTNQTFGEN
                     TQSPVEMVNWNWMLPGFVNYHEGVDGLKTGTTDFAGACFVGTINKDGRRIITVVLNAD
                     NHEENPSARFNETSRLMDYCYDNWSEKELGKANASIPSLKDIKVKDGKETSVNVALKS
                     PVKVWVENGMDTGKLTITPTIDKKKVTDDQVTAPINKGTTVGSATIQLADDKLGYLEA
                     NQEPSTDIITTSTVEKANIFVIGWRHVTDFFSNLF"
BASE COUNT         1122 a          644 c          643 g         1142 t
ORIGIN      
        1 gagatagtat atgaacaaaa aaagatttaa acgtattgaa agaaaatttg cattaactta
       61 tgaccaagct aagtatttga aaacactatt tccaaaacag gtagttcggg ataaatatag
      121 tgatcatttt atttcttcct tgtattttga tacaaacgat catcactttt taaaagaaat
      181 cactacagaa aagtcctatc gagaaaaatt tagaatcaga aaatatggag atcctcagga
      241 catatcgcct atctacttag agataaaaag aaagtttcaa ggaatgacag ataaacgtag
      301 aataaaatta gcgcatcatg agctcgatga tatgttggaa aatatcgaat tggcagaaac
      361 cacattcagt aatttagaag aagagtatat ttttaatgaa atacagtggt tgttcagaag
      421 gttggcattg gagcccaaag tatatatcta ttatttaaga gaagcatttg taaatagaga
      481 ttcagaagat ttgcgaatca cttttgatca aaatatccat taccgtctaa tgaacttaac
      541 gacctgggaa cactgtacag aagacaatct ggtagcgcca gaaatcgatg tgctgatgga
      601 agtaaaaacg acaggagaaa tacctgaatg gttacaggta ggattggata ggatgggtaa
      661 aaaagagata aaattttcta aatataaaca agtctataaa agatatatcc aagaggaccc
      721 gctctatgtt caatagtgtc ttaaaaaatg agccttctca actcacacta gatgatttct
      781 ttttatgtat ggtggtgtcg attttgctag gcattctgat tgcttacacc catatgtatc
      841 aaaacaactg tacaaaaaac ttcatgtttg ccttgattgg gttacctact ttagtacata
      901 ccgtcatttt gttgatcaac ggtaacttag gcacggggat cgctgttcta ggcgcattta
      961 acttagtgcg atttcggtct gctcaaggga attcaagaga tattttcagt attttttggg
     1021 caacgtcgat tggtttagcc acagggacgg gatatattgt ctatgcacta ttcgtgtcta
     1081 gtatagttgc tgctatccaa gtcatcatct tcacgcttcc aatcaaaaat aaacatacag
     1141 tttttaatac tgaaaaaacg atcaagctaa aagtttctaa atccttgttt ctttcgagta
     1201 ttattgaaga aaaaattgca gaatatacag aaaactgcaa tttaaaaaaa gtaaaaatca
     1261 ataaagaaac ttgttcctta cactatatag taaaagtaaa aacgagtatg cttgaaaaag
     1321 aattggttcg aacgatcatg gatcatccag aagtcatttc gatcactgta ggtatggata
     1381 aatcgaataa attattgtga taaccaagaa agtacttaac aatgaagtaa gaccccagtt
     1441 ccaaagttta gcggggacac ttcaataaag tactttcttt tttgtcttca gcaactactg
     1501 gtaaaactga gagcaagctt tcgtgagagt acagcgataa agatcaacct ccctttttat
     1561 ctcgagcaat ttccttgcaa ggctgtttcg ttgatcttaa acgagttagc aacagtcaaa
     1621 gatgcgatgg ccataaaaaa attgcattaa gttggctggt atctgatagc tgggaatcta
     1681 aggaagacta tccattctgt ttcttaaatt ccgctagttg atagatatca gccaattgtt
     1741 ccagatgatc aacatcaaag ataaccgaaa gatgtttcct tttattccca tttgcttgaa
     1801 gagtttttat cttctctttg gttccttcac tcaatgagag tgtgactaat ttctccctcg
     1861 ttttttctgg gaaatgaaag gatactttga aaaatccgtc ccaaatagaa agccagaaga
     1921 tcggttttac tttctgagag tgggacaaaa gtaagaaaca cttttgtctc attctttaaa
     1981 aacgaataaa cggtgggagc agaagcaact ccttcggaaa taagccaaaa ttcacaaaaa
     2041 tttgaaaagc aattttcgtg aatttcttct tatttctcgg agttaaacac ttctgtccca
     2101 acctcttgta ttcacctttt gatagccaag cttttccatc attataaaac cgcaaatttc
     2161 aaaaaaaaac gaacaataca aaaagcgtat tgctcgtttt tctcttcagt ttctcacacc
     2221 taaacgactt ttgtctcgac ctctttttgg tgactattca tgatgttaaa ataaattact
     2281 gaaaaaatct gtgacatggc gccaaccaat gacaaaaata ttagcttttt caacagtaga
     2341 agtggtaatg atgtctgtcg aaggctcttg gtttgcttcc aaatagccta atttgtcatc
     2401 agctaattgg atcgttgctg agccaactgt agtgcctttg ttgataggag cggtcacttg
     2461 gtcatccgtt actttctttt tatcaattgt tggagtaatc gttaattttc cagtatccat
     2521 tccattttcg acccagactt ttacagggct ttttagcgca acattgacgg aggtttcttt
     2581 tccatctttc actttgatgt cttttaggct tgggatgctt gcatttgctt ttccaagttc
     2641 tttttctgac cagttgtcat aacaataatc catcaatcgt gatgtttcat tgaatcgggc
     2701 acttggattt tcttcatggt tatctgcatt taaaacgact gtgatgattc gtcggccatc
     2761 tttgttgatc gttccgacaa agcatgcacc cgcaaaatca gttgtgccag tttttaatcc
     2821 atcgacacct tcgtgatagt ttacaaaacc aggtagcatc cagttccaat tgaccatttc
     2881 aaccggtgac tgtgtgtttt cgccgaatgt ttgatttgtg gtactcgtga tttttaagac
     2941 ttcaggataa tctaagatga ggtgacgagc aacaatagcg acatctttcg cagacagttg
     3001 attttcatct gtttcaccag tattctcagg tcgattctca cctaggtaag aattgtttaa
     3061 acctgatgca ttgacgatcg ttgcatcttt gataccccaa tcttttagtt gtttcttcat
     3121 tagctcaaga aatttacttt cactgcctgc aactttttca gctaaagcaa ccatggaagc
     3181 attggctgat tggatgaaag ctgagtcaac aagttctttc actgtataat cattttcttt
     3241 atgcaaagga acattcgata gatccggcac gacacttaat ttttctgcat aatccgaaat
     3301 ggctacctta tcttcccaag ataatttacc ctctttgatt tgttcaagta cgatatatag
     3361 accaatgatc tttgtgatgg aagcaattcc catcgttttt tcgccatcct gatcataaag
     3421 gatttttcca ctttctgcat ctacggcaaa ggcagctttg gcatttattt taaatgcgtc
     3481 gtctgcttga acagttgcgc taggcaagaa agaagtgata cataagataa gacttgctaa
     3541 tagtacaata a
//
LOCUS       DAXWOO010000204         3546 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.208, whole genome shotgun sequence.
ACCESSION   DAXWOO010000204 DAXWOO010000000
VERSION     DAXWOO010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.208"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            58..510
                     /locus_tag="ACGE70_002652"
     CDS             58..510
                     /locus_tag="ACGE70_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694246.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            524..1213
                     /locus_tag="ACGE70_002653"
     CDS             524..1213
                     /locus_tag="ACGE70_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694247.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1338..>3546
                     /locus_tag="ACGE70_002654"
     CDS             1338..>3546
                     /locus_tag="ACGE70_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFF8694248.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGII"
BASE COUNT         1202 a          467 c          746 g         1131 t
ORIGIN      
        1 caattatctt atcgtagata tatattataa atttaataag aaagcgaggt gagaatcgtg
       61 aaaaaaacga tactaagtat tttttcagta tttacaatat taattggggg ctattttctt
      121 ttgaatttag attctgttaa ttcaaacgct caaataaaag atcaaagagt agctgaacat
      181 aataataaaa aaatgagttt gaccaaaaat atctattcta tgtcagatga agaattgtat
      241 aaagaatttg gtgatttatc cggggaacag ttagaagaaa gtattccagg aattagtgac
      301 tcagataaaa ttgttgtctt gtctgctggt gggcaattaa tgggagatgg caaatccgaa
      361 ttagttagtt ataaacaaaa tcacagacag tttattgatg ttaatactga ttccaacgct
      421 atttcgatta aggatctaaa agagctttta aatagtaaaa gaaatgaatt aaagcagata
      481 aaagaaacta attcagtgga attagagtaa aggaagattc attatgaaaa agatttttgg
      541 tgtttctgtt gggctactta tgatgttaac atttggaaat gcagtatatg ctgctgagaa
      601 agaagttatt tcagatccag agcagtacgc ctcacgatac tttgctgatt ttgaagaaga
      661 agataaagta cttgcattgc cagaaggtgg ttttttaaaa ggggaagcta aaattgtatc
      721 tatggataat ccagatataa ttcaaggttc atataattca gaaactgatc ctgcttcagt
      781 ttcagtgaca gaagcaaaaa aaattgttgc tgaaaaattc aaaaatgata ttgaaactac
      841 tccaattatg cctcgaggag catttccacc cgatgttggt actagcggac accttcttgt
      901 actagcagga ggaggaagct ataaatcaca gccatttagt ggtgcgggat ggcgttttgg
      961 aggtatgggt gtaaaaccag ctgatggtac aggcaacttt ttacgttgga aatcctacat
     1021 tgacgatgga agagtaggaa atgtaaatca agccaacgca actaaatctg gttctattca
     1081 aggaatggca ttgtatgctc gtgaaggatt caaatacttt gcaccaaacg ggcctggttg
     1141 gacaatgaat ataggtattt attacactta taatcctgtt gcaggtacgt attatgttgt
     1201 tcaaaatatt taataaaaca gcacaaagag atttctttgt gctgttttat tatttttagt
     1261 tgttgacaaa cactaaaata taaaatataa tcaacacata aacgaacgta tgttcggcaa
     1321 gaaagcaggg gatttttatg gatgagatgg ttttaagtac tcagaaatgg ctaaacaaga
     1381 aatacagtaa tgttacaggg tttgataagg ttccagaaaa tgggcgaaca gggtggccta
     1441 caatctatgg acttattgaa ggactacaag tagaacttgg tattactaat ctagtagcta
     1501 attttggacc taccactgaa aaaatgtacg ataatcaggt tactcctaaa tggggcaaaa
     1561 atctacctaa aaatattgta tttcttattc aaggtgcctt ttggtgtaag gggattaatc
     1621 caggtggctt cgatggggta tatactccat atttagatac tgctgtaaaa gagttgcaga
     1681 ctgatgcagg gtttaatgga agcgctgtaa ctggagtttt ggatgcgaaa tgggccaaag
     1741 cgttatttga tatgagtgct tttgttcttg ttcccggcgg agatagtaaa attagaaaaa
     1801 tgcagcaatg gttaaatgta aattatttag aatacacggg aataatgcct tgtgatggta
     1861 tttatcaaag aaattcaaat caagcattaa tttttgcatt acaagctgaa ctaggatatt
     1921 ctcccagtga agcaacaggg tcttatggta atgggacaac tagtaaaact tatcctgtgt
     1981 ctgaaggtaa ctcgggaaac tatgtaagaa taattcaata tggattatat gttaatggtt
     2041 attttgaaga cggtacattc gatggtattt tcactaaata tatgggttta gaagtactgg
     2101 cattccgaaa atttatggta ttgcctgaat atactgaaat agcagatgtt gttgtaataa
     2161 aaggactatt gtctagcgct ggagatatta gaagatctgc agatggcgca gatacttcta
     2221 cgcaattaac tagatctcaa atccaaacat tggttgataa tgatataaaa atagttggta
     2281 gatatcttac tggtactgtt ggaattggaa aagatgaacg aaataagtat ctaactacag
     2341 aggaattgaa taatattttt tctaaaaatt tatctgtgtt tcctatctat caagatggag
     2401 gtgcagcatt agcttatttt acttatgatc gtggcttatc agatggtaag aaggctatag
     2461 atgcagcaaa aaatctaaat ataccattaa caacagttat ttattttgct gttgatctag
     2521 acatgttagg ggaagaaatt cttgcatatg ctggagaata ttttaagggg gtatctgctg
     2581 taatgtcata tagcgggtat caaacgggtg tttacggaac aagaaatgtt tgttctcaaa
     2641 taatcaataa cgggtatgct tcgtttgcat ttgtttcaga tatgtcgact ggttatagtg
     2701 ggaatttagg ttttcctatg cctagacaat ggtcttttga tcaatttgtt gaatttacca
     2761 taggaagtgg caacggagct gttggtatcg atttaatcgc tacatcgggt agagatagcg
     2821 gatttaatga gttatcaaat gatactaata atgataatta cattgccaaa tacaatcaga
     2881 aggttataaa tcagatggta cgtgcgtatc aatatctatc acaagctggt cttgataatc
     2941 cttggaaccc tcatttgaca ttttatagat atgtaaatta ttctggtttg tcatgggaca
     3001 tcatttcttc tcctgtaaca gagcatgata gaaaaattta tgatgaatat aggaataaat
     3061 taactaatag cgagggcctg tataattatt ttatagatcc taatactggt tcaataatag
     3121 gtttgcctca cttaattgtg acgttacaat cacagatgtt tctagccgac acaatcaatg
     3181 atagtgtttc tgactttgca ggttggttag gagatttaat gacttgctgg ggtgaagtta
     3241 agaaattagg aatacaaatg gagcaaggtg tatttgcatt agtaggtacg gctgcaggtg
     3301 gaacatttag tctagaagat ttataccaag atgttgacgc ggtaaatgtt tataccttag
     3361 caaaaagaaa ttctgattca acagctttat cggctttaaa aacgtactat tgtgataagg
     3421 gtgctattga tagatttaat caatttgtta agaacagatt tggttcttca gataaaatcg
     3481 cttcagtaac aaatagttta ttagaaaatg acgaaacagt tgatatctta ggtgtaggga
     3541 taattt
//
LOCUS       DAXWOO010000205         3541 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.209, whole genome shotgun sequence.
ACCESSION   DAXWOO010000205 DAXWOO010000000
VERSION     DAXWOO010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.209"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            190..1635
                     /locus_tag="ACGE70_002655"
     CDS             190..1635
                     /locus_tag="ACGE70_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HFF8694249.1"
                     /translation="MLGVILMMRKQSIEGRNQFAMLTIDDLVPKDHLVRKIDAAIQFD
                     FIYPIVESTYSTYGRPSIDPVVLIKLVFIQYLFGIRSMRQTIKDVETNVAYRWFLGYS
                     FEDPIPHFSTFGKNYVRRFRETTLFEDIFSHILEQAVKAGFVTEDNLYIDSTHIKANA
                     NKHKFTKEMTYGQAKAYQDELEDEINKERIAVGKRPFTWDTESELSLQKISHSDPESG
                     YYVKGEREKQFAYSAHTSCEDNGFILSTLITPGNVHDSQVAIDLVKQSKRAFPNIKRV
                     VADAGYKTPKFVHFLSHLKLYPILPYTAPHGVKGMFRKKDFVYDAYFDCYLCPNNQPL
                     LFSTITRDGYRTYKSNPKFCVSCPLLENCTLSTKHQKIVTRHIWADLMDELEHQRHTA
                     LNREIYKKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLTFAAMNLKKLATWLW
                     KTKKPMAFYFKIMQRLTKRSGLITNSFLSTI"
     gene            complement(1652..2440)
                     /locus_tag="ACGE70_002656"
     CDS             complement(1652..2440)
                     /locus_tag="ACGE70_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694250.1"
                     /translation="MSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWISAVGEEN
                     IEFEIKNMDDLKAKFPFIASKLSQVAEDKSWNELVSFSEKIYDITQEDECVSWEMNNQ
                     YKKIDAFVKSRIEGTAFEFEGIDTADNWLNKESFIKSIADDLEKQWSKEDYAEYLKTE
                     RDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYIVEEALSQYSSDE
                     EVDEETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLLIDQNDIE"
     gene            complement(2546..3346)
                     /locus_tag="ACGE70_002657"
     CDS             complement(2546..3346)
                     /locus_tag="ACGE70_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694251.1"
                     /translation="MVGVSKTALDSLAKLFSSRFDGAEFVEYLSQNESGKTLIETMKK
                     DYLNENDFPTWTNIEDATWDVVCTIYSPWVTIADFVLDKIGGDTYPDAKSLATSDFKT
                     FYGYLIGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYANDREKVKLLEGKLSEEELGK
                     MVQEINEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDG
                     YLRSYSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
BASE COUNT         1110 a          665 c          500 g         1266 t
ORIGIN      
        1 caatttaaaa aaggatggca aaaaattatc aagttaaaaa aataaatttt gaaccaaatt
       61 atttttaata aaatagctag tttttttatc aaaaaaaata gagcttttca gctctattca
      121 gactgtagac aaactcctaa atttggagtg ttgtttgcag ttttttgttt tatactaatt
      181 gtataaacta ttctgggagt gattctaatg atgagaaaac aatcgattga aggtagaaat
      241 caatttgcta tgttaacaat tgatgatctt gtacctaaag atcacttagt tcgaaaaatt
      301 gatgctgcga ttcagtttga tttcatttat cccattgtag aatctacgta ttcaacctat
      361 ggtcgtccca gtattgatcc agtggtctta attaaattag tatttatcca atatttattt
      421 ggtatccgtt cgatgagaca gacaattaaa gacgttgaaa caaatgttgc ttaccgttgg
      481 tttttaggtt attcattcga ggatcctatc ccacacttct ctacttttgg taaaaactat
      541 gttcgaagat ttagagaaac aactttattt gaagatattt tctctcatat tctagaacaa
      601 gctgtaaaag ctggcttcgt tacggaagac aacctttata ttgattctac tcatatcaaa
      661 gcaaatgcca ataaacataa gttcacgaaa gagatgacct acggtcaagc aaaagcttat
      721 caagatgaat tagaagatga aataaataaa gaacgtattg ctgtaggaaa acgtcctttt
      781 acatgggaca cagaaagtga gttgagtctt caaaaaataa gtcattcaga tccagaaagt
      841 ggttactatg ttaaaggtga acgagaaaaa caatttgctt attctgcaca tacttcctgt
      901 gaagataacg gttttatctt atctacatta atcacaccag gaaatgtgca tgacagccaa
      961 gtcgcaatcg atctggttaa acagtcgaaa agagcttttc caaatataaa gcgtgtggtt
     1021 gctgatgcag gatataagac gcctaaattt gttcattttc tgtcacactt gaaactctac
     1081 ccgattcttc catatactgc acctcatgga gtgaaaggaa tgtttcgtaa aaaagacttt
     1141 gtttatgatg cctattttga ctgctattta tgtcccaata atcaaccgtt gttattttca
     1201 actatcactc gtgatggtta tcgaacctat aaatcaaatc caaaattctg tgtgagttgt
     1261 ccattacttg aaaattgtac tttgagtaca aaacatcaaa aaatagtgac tcgacatatt
     1321 tgggctgatc ttatggatga acttgaacac caacgccata cggcgttgaa tagagaaatt
     1381 tacaaaaaga gaaaacaaac cattgaacga atatttgcag atgcaaaaga aaagcatggt
     1441 atgcgttgga cgaaatatcg aggtcttgaa aaagtcgcca cgcacacgat gcttacgttt
     1501 gctgccatga atttaaaaaa actagccaca tggctatgga aaacaaaaaa gcctatggct
     1561 ttttatttca aaattatgca aagactaaca aaaaggagtg gcttaattac caactccttt
     1621 ttgtctacaa tctgaataga gcttttcagc tctattcaat atcattttga tcaattaata
     1681 atcgtacact ccaattaacc gagttttgat cactcaaata tttataatct ttgtcatcaa
     1741 aaaccagttg ccaaactctg gtacgaacag tttcttcgtc cacttcctca tctgaagagt
     1801 attgcgataa tgcttcttca actatatact ttacaacttc ctcaaaatca gtaggaactt
     1861 tatcaataac cacttcatag tcttcttgtt ttacgatatt gggatcaata tgttcatcca
     1921 aacatacctc agtgagtttt tctttcactt ctgtatctct ttctgttttt aggtactctg
     1981 catagtcttc cttagaccat tgtttttcaa gatcatccgc tatactttta ataaaggatt
     2041 ctttatttaa ccaattatct gcggtatcta taccttcaaa ctcaaaagct gttccttcta
     2101 tacgtgattt gacgaaagcg tcaattttct tgtattgatt attcatttcc catgaaacac
     2161 actcatcttc ttgcgtaata tcataaattt tttcggaaaa actaactaat tcattccacg
     2221 atttatcctc ggcaacttga cttagtttcg atgcaataaa aggaaatttt gcttttaagt
     2281 catccatatt cttgatctca aattcgatat tttcttctcc tacagctgaa atccatgctg
     2341 aaagattgat tgcaatatcg ctaaagtctt ctacattttc gacatttcct gtattatctt
     2401 ttattactgc ttctaaagcc atatcggttg ttgcactcat ggtttgatct cctctcataa
     2461 tagagcaaaa agccggttta accgactttt tgcatggtac gttttgtttg gtagttttct
     2521 gcttgtgttt atgttgaaat aatccctata gctcttcttt taatagttct tcaactttat
     2581 ctcttaactc tccctgacca ctctctaata gatttaaata gctcatcaga atatccgttt
     2641 catacgtttt taattcagct ctatactcac tttctgaata tgatcgtaaa tacccatcat
     2701 tgctaaatgc aactagatca tcttgaaagt tccattctcc taaatttgtt ttacttactg
     2761 cttcccatgg actattacta aacatttcat taaagaaatc ttcgtcattt tgatatacct
     2821 tcaattcagg aaaatagttg ctgtcttcat tgatttcttg caccattttt ccaagttctt
     2881 cttcagataa tttaccttcc aataatttta ctttttctct gtcatttgca taagccattt
     2941 gaacctctcc tttttctttt tcaatttctt caggtgtttg ttttggcgta actttcaaat
     3001 ctattccatt gcctattaga cctattagat aaccatagaa cgttttaaag tcacttgtag
     3061 caagagattt ggcatcaggg tatgtatccc ctccaatttt atctaaaaca aaatcagcaa
     3121 tcgttaccca aggagaatat atcgtacata ctacatccca tgtagcatct tcaatattcg
     3181 tccatgtcgg aaagtcattt tcattcaaat agtctttttt cattgtttcg atcagagttt
     3241 ttccactttc gttttggctt agatattcaa caaattctgc accatcaaac ctgctactaa
     3301 atagtttagc taatgagtct aaagctgttt ttgatactcc caccattact acacctccta
     3361 ttaatatttt acgatgactt gtttttcatt cacaaataag attttctaca tcttatctat
     3421 tgctttatag cttttccata cattatttct aaatacaacc aataacccta ttaaattaag
     3481 cttttgagtt tatcttttct aataatattc tgataaatat tctgataaaa aaatttattg
     3541 t
//
LOCUS       DAXWOO010000206         3518 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.210, whole genome shotgun sequence.
ACCESSION   DAXWOO010000206 DAXWOO010000000
VERSION     DAXWOO010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3518
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.210"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            41..1756
                     /locus_tag="ACGE70_002658"
     CDS             41..1756
                     /locus_tag="ACGE70_002658"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8694252.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            1759..>3518
                     /locus_tag="ACGE70_002659"
     CDS             1759..>3518
                     /locus_tag="ACGE70_002659"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8694253.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTA"
BASE COUNT         1047 a          699 c          710 g         1062 t
ORIGIN      
        1 ttttttcaat tgtggacaaa taaataatag gaggaatcat atgattcagt tattacgtta
       61 tgcaaaagac tatcgtaagc aaatcatttt gggcccgttc ttcaaatttc ttgaagctgt
      121 cttcgaactt gtcctgcctt taatgatggc ttccttgatc gataatggac taaaaatgaa
      181 tgaccgagga aaaatcattg aaatgggctt atggatggtc gctatgtctg tgattggtct
      241 gatttgtgct atcatttgcc aatactatgc ttcagtagct tcacaaggat ttggtacaga
      301 gttacgtaat cagctaatca aaaaaatcaa tactttctct cacaacgagt tgaatcattt
      361 tggaacagat accttgatca cccgaatgac aaacgacatc aatcagcttc agctagcttt
      421 agcaatggtc attcgtttat tgatcagagc accgttttta agtatcgggt cagtggtcat
      481 ggcttttgtg atcgactggg aagtaggtct gtttttctta gcattattgc ctattttctc
      541 aatcatttta ttttttatca tcaaaaaaac cgttcctctc taccaaaaag tgcaagaaaa
      601 actagatcag ctcaatgaac acgttagtca gaatcttagc ggtgttcgtg tcatccgcgc
      661 atttgcgcag acaaaaaaag aaacagagaa gttcgataaa agcacaaatg atctagcgaa
      721 caattattta cgtgtttcca atatctctgc cctattatca cctgctacaa ccttgatcat
      781 gaatgtcggg atcatctgtt tgctgacagt tggcgggatc aaagtaaata ttggtagctt
      841 gcaacaagga caagttcttg ctttgatcaa ttatatgaat caaatgcttt tggcactgat
      901 cgttgtttca aacctcgtcg tgatcttcac acgtgccgaa gcttcaggaa atcgggtgaa
      961 agaggtgcta gacacagaaa attcgattct tgaagctgag gtcagtacta ctcccgattt
     1021 ttcttctgaa gcaattatcg cttttgacca tgttgacttc cgttatactc ctgattctgg
     1081 tctttctctc caaaatatta catttcagct gaagcgaaat acagtccttg gaatcattgg
     1141 tccaaccgga agcgggaaaa cgactttgac acaattgatt ccgcgtttct atgatgtcag
     1201 ttccggtaca gttataatgg acggaatgga tgttcgttct tggcagctgg atcatctccg
     1261 tcagcaaatt gctcttgttc cccaaacttc tgtactattt accgggacta ttcgtgaaaa
     1321 ccttcaatgg ggtaagaaag acgccacaga aaaagaatgt tgggaagctc tgaaaatcgc
     1381 tcaggcagaa gaatttgtcc gtcagcttcc aaatggttta gatacaaaag tcatggaaaa
     1441 tgggaaaaat ttctctggcg gacaaaaaca gcgactgacg attgcaagag ctttgattgc
     1501 caagccagca ttactgattt tagatgactc tttaagtgcg ttggattatc aaacagactt
     1561 ggatttgcgt aaggcgctac agacttatct tggcagtaca gtgattatta tctcccaacg
     1621 tgtccgctct attcaagaag cacatcatat cttagtgatg gatcaaggta agattgctgc
     1681 tcagggtacc catgaagaat tacttgctca atcagcagaa tatcaggaaa tcgtcgcatc
     1741 acaggaggaa gaatagttat gaaagtaaaa aaattatcct tgcagacgct ccgccgattc
     1801 attccttacc ttactgccta tgcaaaagac atttggctag ctgtgatact aggaatcatc
     1861 aatgggattg caacagttgt gatgacgtta caaattggtc aaagtatcga tcaaatgatt
     1921 ggagaaggac aggtccgctt caatcgactg ttccatttac ttattctatt cggtggtatc
     1981 gttttaatca acgtagtcag tcaatggctc atccaagtgt taagcaatcg gttagcttac
     2041 ttatccgttg caaaattaag aaaagatgca tttgctcatc tgaatcgatt gccacttcgt
     2101 tattatgatc aaaactccca cggaaatatc gtcagtcgat ttacaaatga tatggacaat
     2161 atttccattg caatttctgc ggccttcaat caattatttt ctggagtggc aatcatcatt
     2221 ctttcgttta tttttatgat gcgtctaagt ccacttctga cattggtcgt tctttgttct
     2281 acaccaatta ttttccttgt gagttggatc gttgctcgtg cttcacaaac aaatttcgat
     2341 agacagcaaa ggattattgg ggatatctcg ggctttgtga ccgaacgaat cggtaaccag
     2401 aaaatcgtga aagcttttca gcaagaagaa gctagtcaac agcaatttga actgctaaat
     2461 gctgatctat atgaaaaagg acaacgggca caattttctt cttccttgac aaacccgtct
     2521 tctcgttttg tagatcacct tgcttattta tcgattggtt tagttggcgg cctattggca
     2581 ttgaagagtg gttcgtcaat cactgttggt gtgatttcca gctttacgat ctactcaagt
     2641 cagttttcta aaccatttat cgaactttct ggaatcacga cacaactgca aaccgctttt
     2701 tctggtctag aacgagcatt tgatttgatc gatcagccag aagaaaagcc tgatttgcca
     2761 gatgcctatg ccttggaacc caaaaatatc aaaggaaaag tctcttttga tcatgtttct
     2821 ttttcttatg accccaagca gcgtttgatc gaagatttca attttactgc ttatccaggt
     2881 caaaccattg ctatagtagg aaaaacaggg gccggaaaat ccacacttgt taatttgctg
     2941 atgcgttttt atgacacaac aaaaggaaca attacagtcg atgaccatga catccgctct
     3001 atccaacgtg atacattacg taaaagtttt ggaatggtgt tacaagatac ttggctattt
     3061 gacagtacat tgcgagaaaa tcttcgtttt gggaatccgg aagcaagtga cgcagaaatc
     3121 aatgatgccc taaaagcctc ttatatgtat gaatttgtag aacgtttgcc ggaaaaatta
     3181 gatactaaga ttggcggtca agggttaaag atatcagatg gacaacgcca actgttgacg
     3241 attgctcgaa cgatgatcag taatccccct atgttgatct tagatgaagc taccagctca
     3301 gtcgatacat tgacagagaa aaaaatccaa caagccttct tagcaatgat ggaaggaaaa
     3361 accagttttg tgattgccca tcgcctgtcc acaatcaaaa atgcggacca gatattagtg
     3421 atggatcagg gagctattgt cgaaatgggc acccatgatg agctgcttca aaaagatggt
     3481 tattaccgac ggctctatca agcacaattc acagcaca
//
LOCUS       DAXWOO010000207         3502 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.211, whole genome shotgun sequence.
ACCESSION   DAXWOO010000207 DAXWOO010000000
VERSION     DAXWOO010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.211"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(<1..182)
                     /locus_tag="ACGE70_002660"
     CDS             complement(<1..182)
                     /locus_tag="ACGE70_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFF8694254.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="ACGE70_002661"
     CDS             517..951
                     /locus_tag="ACGE70_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFF8694255.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="ACGE70_002662"
     CDS             974..1294
                     /locus_tag="ACGE70_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694256.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="ACGE70_002663"
     CDS             1435..1626
                     /locus_tag="ACGE70_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694257.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="ACGE70_002664"
     CDS             complement(1697..1954)
                     /locus_tag="ACGE70_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFF8694258.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="ACGE70_002665"
     CDS             2112..2375
                     /locus_tag="ACGE70_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694259.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="ACGE70_002666"
     CDS             2399..2587
                     /locus_tag="ACGE70_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694260.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="ACGE70_002667"
     CDS             complement(2670..2855)
                     /locus_tag="ACGE70_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694261.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="ACGE70_002668"
     CDS             3070..3441
                     /locus_tag="ACGE70_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFF8694262.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
BASE COUNT         1308 a          499 c          700 g          995 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac ta
//
LOCUS       DAXWOO010000208         3399 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.212, whole genome shotgun sequence.
ACCESSION   DAXWOO010000208 DAXWOO010000000
VERSION     DAXWOO010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.212"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..356
                     /locus_tag="ACGE70_002669"
     CDS             <1..356
                     /locus_tag="ACGE70_002669"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFF8694263.1"
                     /translation="INSFQETFYKDKKAIVIEGPLVRTFRHCLFCKESAKQIEKNGKK
                     ISMIILNRSGNKQTYLRLRKQHYHCQSCGKYFTAHTYLVAPSCFISKQVHYTILEELT
                     ERQALKTIAKRCDFQ"
     gene            513..770
                     /locus_tag="ACGE70_002670"
     CDS             513..770
                     /locus_tag="ACGE70_002670"
                     /inference="COORDINATES: protein motif:HMM:NF013753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFF8694264.1"
                     /translation="MTFCPAGEKKDLIAYFMRFTRKAHMGVRYIVTDMNAPYFSLVKE
                     PFPNAQIIIDCFHIGQHLNKFFDRVRKRVMKQLNQKCQRHL"
     gene            857..2095
                     /gene="istA"
                     /locus_tag="ACGE70_002671"
     CDS             857..2095
                     /gene="istA"
                     /locus_tag="ACGE70_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HFF8694265.1"
                     /translation="MRKDIREGVMIYVINEIKPNYAALAKQYDCDYRTVKHAYEEAQV
                     KESKPPERKKRPSKLDPYREIIQDKIKDQCSAYSIFKFIEHKGFGGSYSLVKTYCRQI
                     KQAKVHKATVRVEHSPGLSAQVDWKEDMRLISNQGEIFEFNIFLYVLPYSKKKYVTLT
                     FDRKQDTLFECLDDAFYQTGGVPKEIWFDNMKTVVDHSRSQFSKPHFNKRFYAFSKDA
                     GFTPIACRPFRPQTKGCVEALARTVDRLRVYNHEFYDAVELCHLVNNLCVELNNEVSQ
                     STDQTPDERWQTNEKEHLHPYQKNLLNPYFEDFIRRKVTKEEMVIFRKCRYSVAPRYI
                     GKEVEIELSDKEDQVQIYYNGELIRSHPLTTKQLNYHEEDYFQILKSDVMSHKEDDGI
                     RAYTRESLKQYDLVEVADEE"
     gene            2085..2825
                     /gene="istB"
                     /locus_tag="ACGE70_002672"
     CDS             2085..2825
                     /gene="istB"
                     /locus_tag="ACGE70_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HFF8694266.1"
                     /translation="MKNSHYQELMTNLETLGLNHMREYVPNYIEVANREELSLTEALL
                     ELTRREVDHQNKQKMNRVVARARFSKRTSLEEFDFAFQPSINKREVLDLRHMGFVEKH
                     ENLVFIGSPGVGKTHLTIALGIEACRQGYRTLFITCHELLLRLRTAYEKGTLERVLKR
                     YARYDVLIIDEIGYLPIQKQEADLFFQLLAMRYEHRSTIITTNIVLSRWGELFQSSEI
                     AAAILDRLVHHVKVFKITGKSYRMRDKI"
     gene            3244..3399
                     /locus_tag="ACGE70_002673"
                     /pseudo
     CDS             3244..3399
                     /locus_tag="ACGE70_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1091 a          689 c          666 g          953 t
ORIGIN      
        1 aaattaattc ttttcaagaa actttttata aggacaagaa agccatcgta atcgaaggtc
       61 cattagttcg cacttttaga cattgtctat tttgtaaaga atctgcaaag cagatcgaga
      121 aaaacggcaa gaagatttca atgattatct taaatcgcag tgggaataaa caaacctatt
      181 tgcgtcttag aaaacaacat taccactgcc aatcatgcgg gaaatatttt actgctcata
      241 cgtatcttgt cgctccttca tgttttattt cgaaacaagt tcattacacg atattagaag
      301 agttgaccga gcgtcaagca ttaaagacaa ttgcgaaacg ttgtgatttt cagtaacgac
      361 agttcaacgg acattatctg ctctatcggc aacgctgcga cagaagctag actatttacc
      421 tcattgttta ctattcgacg agtttcgctc tctttcaatt ttccatggga agttctcctt
      481 ttcttgtatg aattgagaga cagggaagct ctttgacatt ttgtccagcc ggagaaaaaa
      541 aagatttgat tgcttatttc atgcgtttta caagaaaagc tcatatgggg gttcgataca
      601 ttgtcacaga tatgaatgcc ccttattttt cattagtcaa agaacccttc ccaaatgcac
      661 aaatcatcat tgattgcttt cacattgggc aacacttgaa caagtttttc gatcgtgttc
      721 gaaaacgtgt gatgaagcag ttgaatcaaa aatgtcaacg gcacctttaa aatgtaggaa
      781 aacggcaatt taaaattgtt ggtttaccta catttttgta tgctcttctt atcccaaatg
      841 aaatggaggc tcagggatga gaaaagacat acgcgaagga gtgatgatct acgtgatcaa
      901 tgaaatcaaa cccaattatg ctgcactagc aaaacaatac gactgcgact accgaacagt
      961 taagcatgct tacgaagaag cccaagttaa agaatcaaaa cctccagaac gtaagaagcg
     1021 tccaagtaag ttagatccct acagagaaat catccaagac aaaatcaaag accaatgcag
     1081 tgcttacagc attttcaagt ttattgagca caaaggattt ggaggaagct acagtttagt
     1141 aaagacctat tgtcgccaga ttaagcaggc gaaagtacac aaggcaaccg tccgagtcga
     1201 acatagccct gggctttctg ctcaggttga ctggaaggaa gatatgcgac taatcagtaa
     1261 tcagggcgaa atttttgagt tcaatatctt tttgtacgtc ttgccatatt ccaagaaaaa
     1321 atatgtcaca ctaactttcg accgaaaaca agacacactc tttgagtgct tagatgatgc
     1381 gttttatcaa accggtggcg ttcccaaaga aatctggttc gataatatga agacagtcgt
     1441 tgaccattct cgtagtcagt tttccaaacc gcatttcaac aaacgttttt atgctttcag
     1501 taaggatgcc ggcttcacac caattgcctg tcggccattt cgtccgcaaa caaagggctg
     1561 tgtcgaagct ttagccagaa ctgttgaccg cctacgggtc tataatcatg aattttatga
     1621 tgccgttgag ctgtgtcatt tagtgaataa cctttgtgtt gagttaaata acgaggtttc
     1681 tcaatcaact gatcaaacac cagatgaacg ttggcagacg aacgaaaaag agcacctgca
     1741 tccataccaa aaaaaccttt tgaatcccta ttttgaagac tttattcgcc gtaaagtcac
     1801 caaggaagaa atggtcattt tccgtaaatg caggtactct gtggcgcctc gctatatcgg
     1861 taaagaagtc gaaatcgaac tctccgataa agaagaccaa gtacaaatct attataacgg
     1921 agaactgatt cggtcgcatc cattaacgac aaaacagctg aactaccacg aggaagacta
     1981 tttccaaatc ttgaaatccg atgtcatgag tcacaaagaa gacgatggaa tcagagcgta
     2041 tactcgtgaa agtctcaagc aatatgatct ggtggaggtt gccgatgaag aatagccatt
     2101 accaagaact gatgaccaac ttagagactc ttggtttgaa tcatatgcgg gaatatgttc
     2161 ctaactacat cgaagtggcc aatcgagagg agctttcttt aacggaagct cttctcgagc
     2221 tgacacgcag agaagttgat catcaaaata agcaaaaaat gaatcgtgtg gtggcacgcg
     2281 cccgcttttc caagagaacc agcctggaag agtttgattt tgccttccag ccaagtatca
     2341 ataagcgcga agtgctagac ttaaggcaca tgggctttgt ggaaaaacat gagaacttgg
     2401 tttttatcgg cagtcctggt gtaggcaaga cacatctgac gattgcattg ggaatcgaag
     2461 cctgtcgtca aggctatcgg acactattca tcacctgtca tgagctgctt ttgcgtctgc
     2521 gaacagccta tgaaaaaggt accctagagc gtgttctcaa acgatacgct cgctatgacg
     2581 tcttgattat tgatgagatt ggctatcttc cgattcaaaa acaggaagct gatttattct
     2641 ttcagcttct tgccatgcgc tatgaacacc gctctacgat cattacgacg aacattgtct
     2701 tatctcgttg gggggagttg ttccaaagtt ctgagatcgc agctgccatc cttgaccgtt
     2761 tagtacatca cgtcaaggtg ttcaagatca caggcaagtc ttatcgtatg agagacaaga
     2821 tctaaagtgt cgattgtcga caattactat tgccgaaaac ctacattttt atcctgccca
     2881 aaacctacaa ttttaagctg cccttgacat caaaaagaca cttcacaagc taagtgctat
     2941 cgacaattga aacctttgta taaattactt ctataatcag atgacgagtt ggattataca
     3001 acattcaaga aatggcagaa ttttcaatgg cgctatttaa cagaaagtga agtagttgat
     3061 tgtctaccct caatctctta tgaacttaaa atggcttagc agtattatca aaagctgctt
     3121 aacaattatc acaagaaaca gctagctact tcgagaaatg accaaagaag ttccctcgaa
     3181 acttcactat cttaaaaagc ctttttcata tctgaaaaag gcttttaact agcattttcg
     3241 aaaaagtatt ttaatacaaa attagagaat cttcgcacac atatcaaaac attgaaacga
     3301 tttgcttatg gattttgcag ttttaccaat atgcgcttgt ggatttttct aatcaacggg
     3361 ttaataagct atgcattaaa agctagtaaa tcttactaa
//
LOCUS       DAXWOO010000209         3342 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.213, whole genome shotgun sequence.
ACCESSION   DAXWOO010000209 DAXWOO010000000
VERSION     DAXWOO010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3342
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.213"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            92..1102
                     /locus_tag="ACGE70_002674"
     CDS             92..1102
                     /locus_tag="ACGE70_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HFF8694267.1"
                     /translation="MKILITGGAGFIGFHLSRKLLKETENQIVAIDNLNDYYDVQLKE
                     DRLTILYDYNNFKFIRMDLLEKEKLDSLFESEGFDIVIHLAAQAGVRYSIENPYAYID
                     SNIIGFLNILEACRNFPVKHLLYASSSSVYGGNTKVPFSTEDSVDHPVSLYAATKKSN
                     ELMAHTYSHLYKIPTTGLRFFTVYGPFGRPDMAYFSFTKNILSGKEIKIFNHGKMERD
                     FTYIDDIVEGITRLINKVPEPNSDWTDIDDLSSESWAPYKIYNIGNNKPVALLEFVET
                     IEKYLGVKANKIFLDMQPGDVVRTYADIEDLEEKINFKPNITLDEGIKKFVEWYTVYY
                     LS"
     gene            1470..2894
                     /locus_tag="ACGE70_002675"
     CDS             1470..2894
                     /locus_tag="ACGE70_002675"
                     /inference="COORDINATES: protein motif:HMM:NF026018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFF8694268.1"
                     /translation="MDSSSNKSHSIAKKAFIYAIGTLSSKMISVIIIPIYAVYISVAD
                     LGSFDYQNTIAQFLSPVIFGAIWEAILRMGLGKEKNIQKKVVTNSICFVIANTVILAI
                     FLPIIYRMIFPNLEYYQLFVFQLIFTPILSVFQYIVRALGESKIFVMSGIIAAGINLL
                     LLTIFVVTMGMGILGLLLSIIISNVINLIFLIFRGQIFQYFATSSFDFLFLKSMLKYS
                     IPLTLNLTFGWLIVGFSRFYIGTYIGVTENGIFSFAMKFSSIILTLTSIVSMSMIEDA
                     VITSENDDFIERFVEKNTYIFKMYIRLATLSIPIICCAYSFITNPDYKKSLVLVPILV
                     IMACFSGMTTNIGNIFAVYNKTNQLLMISVVSGLLSVVGTYLLGYSFGVIGVSFATLL
                     GTLVLFGLRYYFGRKIAYYAFDWKRISLLLVLYSAIAFVCIHRGFYFNLFLTIIFSAI
                     FVFTYKQLIITYLKKFFRIKMAKR"
     gene            2980..>3342
                     /locus_tag="ACGE70_002676"
     CDS             2980..>3342
                     /locus_tag="ACGE70_002676"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694269.1"
                     /translation="MIFGKKHSLVEVPEFGSEPYLIEIGDNFRCSFGVKFITHDGGVW
                     VLRKMSSVSESTSKIGRIKLGNNIFIGIYSIVFPGVSIGDNVIIRANTLVNKDIPNNS
                     VVAGSSCKVISTIEEHYEK"
BASE COUNT         1109 a          437 c          612 g         1184 t
ORIGIN      
        1 ctaaataata aatgtaatac gagtataaag aagaaagcaa ttttgggttt aaccctattg
       61 gttcctttca gaaaaaggtg gatgaataaa aatgaaaata ttaataacag gtggagcagg
      121 atttattggt tttcatttgt ctagaaaatt actcaaggaa actgaaaatc aaattgtagc
      181 gattgataat ctcaatgatt attatgatgt ccaattaaaa gaagatcgat taacgatact
      241 gtacgattat aataatttta aatttatcag aatggatcta ttagagaaag aaaaattaga
      301 ttctctattt gaaagtgaag gttttgacat agtaattcat ttagctgctc aagcaggtgt
      361 tagatattct attgaaaacc cttatgctta tattgattct aatattatcg gttttcttaa
      421 tattcttgaa gcctgccgaa acttccctgt aaagcatcta ttatatgcat catctagttc
      481 agtatatggt gggaacacga aagtaccgtt ttcaacagaa gattcggtgg atcatccagt
      541 tagcctttat gcggctacca aaaaatcgaa tgaattaatg gctcatactt atagccacct
      601 ttataaaata ccaacaactg ggctgagatt tttcactgtc tatggtccat ttgggcgacc
      661 agatatggcc tatttctctt ttaccaagaa tattttatct ggaaaagaaa ttaagatttt
      721 taaccatgga aagatggaaa gagatttcac ttacatcgat gatatcgtag agggaatcac
      781 tagattaatc aataaagtac ctgaacccaa ctccgactgg acagacatcg atgatttgag
      841 tagtgaaagt tgggcaccat ataagatata caatattgga aataataagc cggttgcatt
      901 gttggagttt gttgaaacaa ttgaaaagta tttaggtgtt aaagcgaaca agattttttt
      961 ggacatgcag ccgggtgatg tcgttagaac ttatgccgat attgaagatt tagaggaaaa
     1021 aatcaacttt aaaccgaata taaccttgga tgagggcatt aaaaaattcg ttgaatggta
     1081 tacagtgtat tatttgagtt aattttgaaa ggaatattga agatgggtaa ttcatacgat
     1141 ttttttagct ttgttaggta ttttaggaaa ttctactttc aaacgtgacg aactttctcg
     1201 ccgaatagat attttctata actaaaagaa tttctataaa acaaaattgt gtatcaagat
     1261 gtgtgaactg gattctgact gccaatattg attgatgaga ttgaagaaat atatagttga
     1321 tcatggatat ctttatttga aaagatctta gctattcaca tattcaaaaa aatttttact
     1381 acgaggactc acacgataca gaggaattta ggtagttcta attaaaccag aaagtcatct
     1441 atagatgtga gttgagggag gtcatacggt tggatagtag cagtaataaa tcacattcaa
     1501 tagctaaaaa agcttttatt tacgcaatag gaacattatc atcaaaaatg atatctgtca
     1561 ttataatccc tatctatgca gtatatattt ctgttgccga tttaggaagc tttgattatc
     1621 aaaatacaat tgctcaattt ctttctccag ttatttttgg agcaatatgg gaagcaattt
     1681 tacgcatggg attaggcaaa gaaaaaaata ttcagaaaaa agtagtgacg aacagtattt
     1741 gttttgttat agcaaataca gtgatattag ccattttttt accaataata tatagaatga
     1801 ttttcccgaa tttagagtat tatcaattgt ttgtttttca attaattttc actcctatcc
     1861 taagtgtttt tcaatatatt gtaagagcac taggtgaatc aaagattttt gtgatgagtg
     1921 gtataatcgc tgcgggtatc aacctgttac tccttactat ttttgttgtt acaatgggta
     1981 tggggatact tggtctctta ctctctatca taatttctaa tgttataaat ttgatttttt
     2041 tgattttcag ggggcagatc tttcaatatt tcgcaacgag ttcttttgat tttttatttt
     2101 taaagtccat gctgaaatac tctatcccac tcactttgaa tctcactttt ggatggctaa
     2161 tagttggttt ttcaagattt tatattggaa cgtacatcgg agtaacagaa aatggtattt
     2221 tttcttttgc gatgaaattt tcatctataa ttttaactct tacaagtatt gttagtatgt
     2281 ctatgataga ggatgcggtt atcacgtctg agaatgatga tttcatcgaa agattcgttg
     2341 agaaaaatac ctatattttt aaaatgtaca ttaggttagc gactctttct attcctatca
     2401 tttgctgtgc atactctttt ataaccaacc ctgattataa aaaatcatta gttctagttc
     2461 caatattagt tattatggca tgtttcagtg ggatgacaac aaatatcgga aatatatttg
     2521 cagtatacaa taaaacaaat cagttattaa tgatttcagt agtgtcaggt ctattgagtg
     2581 ttgttggcac ctatttatta ggctattctt ttggagtaat tggtgtctca tttgctactt
     2641 tgctcggtac tttagtatta tttggactta gatattattt tggaaggaaa attgcttatt
     2701 atgcatttga ttggaagcga atatcgctac ttttagttct ctattccgca atcgcttttg
     2761 tatgcattca tagaggcttc tattttaatc ttttcttaac gattattttt agtgctattt
     2821 tcgtttttac gtataaacaa ttaattataa cgtatctaaa aaaatttttc agaataaaaa
     2881 tggctaaaag gtgagggtat acgaagtaag aatagaatct ttcgaagaat aaagagaaaa
     2941 ctatttggaa aaaatctgcg agaatgtatt aatgaaggtt tgatattcgg aaaaaaacat
     3001 agtttagtag aggttcctga atttgggagc gagccatatt taattgaaat aggggataat
     3061 ttcagatgtt catttggtgt taaatttata acacacgatg gtggagtctg ggtcttaagg
     3121 aagatgagtt ctgtgagcga gtcaacttca aaaataggta ggatcaaatt gggaaataat
     3181 atttttattg gtatttactc tattgttttc ccaggagttt caattggcga taatgtaatt
     3241 attagagcga acactttagt aaataaagat atcccaaata atagtgttgt agcgggtagt
     3301 tcatgtaaag tgatttcaac tatagaggag cattatgaga aa
//
LOCUS       DAXWOO010000210         3321 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.214, whole genome shotgun sequence.
ACCESSION   DAXWOO010000210 DAXWOO010000000
VERSION     DAXWOO010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.214"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            238..1629
                     /locus_tag="ACGE70_002677"
     CDS             238..1629
                     /locus_tag="ACGE70_002677"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFF8694270.1"
                     /translation="MNKNGEWNAARRIFIILMDVLVLNVSIYLSFLLKFQGEVPVRNL
                     ETFQHSAIFISIIFIALNILLGAYVFYNRMISDIVFITVIGQVLIALGIMVVTFAGRW
                     FAFPRGVILISFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVAPAIQNFQSK
                     KNNKHKVEAVAIDNYYENTKKLIDDMDIVYLASKIPEDEKINIYQLVTKKEKKLFLNT
                     TFENLTMINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLILLILASPFMLIT
                     AILVKTTSPGPIIYKQVRITKNQKEFSIYKFRSMSATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNEENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLLLDLKILLQTIKILFDKMSSQGLDEDENISS
                     LAESAFPKEKTYR"
     gene            1667..2497
                     /locus_tag="ACGE70_002678"
     CDS             1667..2497
                     /locus_tag="ACGE70_002678"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFF8694271.1"
                     /translation="MENKNISILLSLYDKEKPEYLKEALASIFEQTVMPAEIVLVYDG
                     PINEKLQAVVTYFQQKVPHLFTVVKLEKNQGLGIALATGLEHTKNRLVARMDTDDIME
                     PHRLEKQLEIFQEYPEIAIVGSNIEEFTGTLSNVIGRRIVPEYNDEIRVFSKRRNPFN
                     HMTVMFDKNAILEVGNYRPLIGFEDYYLWVRLLKAGYKGYNIQEILVHARAGADMYAR
                     RGGSKYLLPGIKGRYRVWKDGLGSLKDFLIVICGHLVISLLPNKIRGKVYESKLRKTN
                     "
     gene            2469..>3321
                     /locus_tag="ACGE70_002679"
     CDS             2469..>3321
                     /locus_tag="ACGE70_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFF8694272.1"
                     /translation="MNQNLEKQTKLSIIIPFYNAKNTLRRAVDSVLTQGYPNIEILLI
                     DDGSTDGSSDIGDEIANLESKVACYHLENAGPSVARNYGIKEATGNLVGFLDSDDYFL
                     PDCFNNVLKDYHRNHPDIIAFGLSKGKNIENANLFVPEKKIGCSSEEAIEQMFRSKAV
                     DFYVWNKFFKRELFNHIVFPENKLYEDMVPMYEAFRAASKIDILPFSGIFYYQNNDSI
                     VYQSFNSKQYDNILQRKILVDEVKKDYPRLINLANARLVDGYLSTGFKITGKNQNQKE
                     KQKYYNQS"
BASE COUNT         1167 a          465 c          641 g         1048 t
ORIGIN      
        1 tatatgaata ttgtaacttg tgaataagga attagcagga gaatactagt tcagcataaa
       61 ggaagtgatg ggacttctga gacaactttc tgttatgcag aaatacacat ataaacagac
      121 aataaacgct tttccccgtt tcttttaatt tcctttacaa tttggttatt cactaggttt
      181 ttaaactttg ctatggtaag atgatagagg cattatttta tggttaggag tgtatatatg
      241 aataaaaatg gggagtggaa tgcggcacgc agaatattca ttattcttat ggatgtactt
      301 gtattaaatg tatcgatcta tcttagtttt ttgctgaaat tccagggaga agtaccagtg
      361 agaaacttag aaacctttca gcattcagca atttttattt ccatcatctt tattgcgttg
      421 aatattttgt taggagctta tgtattctat aaccgaatga tcagtgatat tgtcttcatt
      481 actgttattg gacaagtact aatagcattg gggatcatgg tagttacttt tgctggccga
      541 tggtttgcat ttccgcgtgg cgtcattttg atctcgttta tcctaggtac gatattactt
      601 agtgtttacc ggatagtcgt ttacaaactg tacttaagag tcagtcgtga taagaaagtc
      661 cttatcgtgg gtttggagaa agatgtcgca ccagctatcc aaaattttca atctaagaaa
      721 aacaataaac ataaggttga agcagtggca atcgataatt attacgaaaa tacaaaaaaa
      781 ttgatcgatg atatggatat cgtctatttg gcttctaaaa tcccagaaga tgaaaaaatc
      841 aacatttatc aattagtgac taaaaaagag aagaaattat ttttaaatac gacttttgag
      901 aatctgacaa tgataaatcc gaatattatg aattttgaag atgaaagcat cattgaagct
      961 tctgggttca gaatccaacc agaatacgaa ctattcaagc gtctctttga tatcatcgtt
     1021 tcgcttattt tactgatcct cgcctcacct tttatgctaa tcacagcgat tttggtaaaa
     1081 acaacttcac ctggaccaat catctataaa caagtacgta ttacgaaaaa tcaaaaggaa
     1141 ttttcaatct ataaattccg ttccatgagt gcgacagctg aagcaaaatc aggaccagtc
     1201 ttggctaaaa gtaacgatgc acgtgtgaca ccagtaggta aatttatccg tgctgtacgt
     1261 tttgacgaat tacctcaaat ctttaatgta ttgaaaggcg atatgtcgat tgtcggtcca
     1321 aggccagaaa gaccattttt cgttgatcag ttcaatgaag aaaatcctta ttattactta
     1381 agacacaatg tacgtgcggg aattaccgga tatgcacaag tatacggaaa atatgtatca
     1441 gattataaca gtaaactacg ttttgatttg ttgtatatca aaaagtattc cttactatta
     1501 gatttgaaga ttttacttca aacaatcaaa atcttatttg ataagatgtc ctcacaagga
     1561 ctagatgaag atgaaaatat cagttcatta gcagaaagtg catttccaaa agaaaaaacg
     1621 tatcgttaga ttggaaaaat tttgagcaaa gaagtaggag agaacattgg aaaataaaaa
     1681 tataagtata ctgctttctt tgtatgataa agaaaagccg gaatatttaa aggaagcatt
     1741 agctagtatc tttgagcaaa cggtgatgcc ggcagaaatc gtattggttt atgatggccc
     1801 tataaatgag aagcttcagg cagttgtcac ttatttccag caaaaagttc ctcacttgtt
     1861 tacagttgta aaattagaaa aaaatcaagg attggggatt gctttagcaa ctggattaga
     1921 acatactaaa aatcgcttag tggccagaat ggatacagat gacatcatgg aaccacacag
     1981 attggaaaaa caattagaga tcttccaaga atatccagaa atagcgattg ttgggagtaa
     2041 tattgaagaa tttactggca cacttagcaa tgttatcgga agaaggattg tacctgagta
     2101 taatgatgaa atccgtgtgt tttcgaaaag aagaaaccct tttaatcata tgacagtcat
     2161 gtttgataaa aatgctattt tagaagtggg aaactatcgc ccattaattg gatttgaaga
     2221 ttattatttg tgggtaagac tattgaaggc gggatataaa ggatataata tccaagagat
     2281 tctagtacat gctagagcag gtgctgacat gtatgctaga cgtggaggaa gtaaatatct
     2341 tttaccagga attaaaggcc gatatcgcgt atggaaagat ggtctaggat cactaaaaga
     2401 ttttctgata gttatttgcg gacatcttgt aattagttta ttaccgaata aaattagggg
     2461 aaaagtatat gaatcaaaac ttagaaaaac aaactaaact ttctataatc ataccttttt
     2521 acaatgcaaa gaatacttta agaagagctg tagacagcgt cttaacgcaa ggttatccca
     2581 atattgaaat cttgttaatt gatgatggat ctacagatgg tagtagtgac attggtgacg
     2641 aaattgctaa tctagagagt aaagtagcat gttatcactt ggaaaacgct ggtccatcag
     2701 ttgctaggaa ctatgggata aaagaagcaa ctggaaattt ggtaggattt ttagatagtg
     2761 acgattattt tttacctgat tgttttaata atgttttgaa agactaccat aggaatcacc
     2821 ccgatatcat tgcttttgga ttaagtaaag gaaaaaatat agaaaatgca aatttatttg
     2881 ttcccgaaaa gaagattggt tgttcaagtg aagaagcaat tgagcaaatg tttagaagta
     2941 aggcagtgga cttttacgta tggaataaat tttttaaaag ggaattattc aatcatatag
     3001 tttttccaga aaataaatta tatgaggata tggttcctat gtatgaagct tttagggcgg
     3061 ctagtaaaat tgatattttg ccattttcag gtatttttta ttatcaaaat aacgatagca
     3121 tcgtttatca gtcttttaat tcaaaacagt atgataatat tttgcagaga aaaatattag
     3181 ttgatgaagt aaagaaagat tatcctcgac ttattaattt ggccaatgct cggttagtag
     3241 acggatatct ttcaacaggg tttaaaatta ctggcaaaaa tcaaaatcag aaagaaaaac
     3301 aaaaatatta taatcagtct a
//
LOCUS       DAXWOO010000211         3281 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.215, whole genome shotgun sequence.
ACCESSION   DAXWOO010000211 DAXWOO010000000
VERSION     DAXWOO010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.215"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..359
                     /locus_tag="ACGE70_002680"
     CDS             <1..359
                     /locus_tag="ACGE70_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFF8694273.1"
                     /translation="SFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGA
                     GMSLLNFTSFLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVI
                     SWLVTLNVYKHSQRDF"
     gene            923..2185
                     /gene="mobV"
                     /locus_tag="ACGE70_002681"
     CDS             923..2185
                     /gene="mobV"
                     /locus_tag="ACGE70_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="HFF8694274.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            2430..>3281
                     /locus_tag="ACGE70_002682"
     CDS             2430..>3281
                     /locus_tag="ACGE70_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFF8694275.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQRFRRMMQYKKINKNLVGFMR
                     ATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPNVKV
                     QMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLISYG
                     GLLKEIHK"
BASE COUNT         1208 a          436 c          676 g          961 t
ORIGIN      
        1 cttcctttct tttagaaaca acatcatggt tcatgacaat tataatcgta tttgttttag
       61 gtgggctttc gttcaccaaa acagttatat caacaattgt ttcaagtagc ttgaaacagc
      121 aggaagctgg tgctggaatg agtttgctta actttaccag ctttttatca gagggaacag
      181 gtattgcaat tgtaggtggt ttattatcca tacccttact tgatcaaagg ttgttaccta
      241 tggaagttga tcagtcaact tatctgtata gtaatttgtt attacttttt tcaggaatca
      301 ttgtcattag ttggctggtt accttgaatg tatataaaca ttctcaaagg gatttctaaa
      361 tcgttaaggg atcaactttg ggagagagtt caaaattgat ccttttttta taacaggaat
      421 tcaaatcttt ttgttccatt aaagggcgcg attgctgaat aaaagatacg agagacctct
      481 cttgtatctt ttttattttg agtggttttg tccgttacac tagaaaaccg aaagacaata
      541 aaaattttat tcttgctgag tctggctttc ggtaagctag acaaaacgga caaaataaaa
      601 attggcaagg gtttaaaggt ggagattttt tgagtgatct tctcaaaaaa tactacctgt
      661 cccttgctga tttttaaacg agcacgagag caaaaccccc ctttgctgag gtggcagagg
      721 gcaggttttt ttgtttcttt tttctcgtaa aaaaaagaaa ggtcttaaag gttttatggt
      781 tttggtcggc actgccgaca gcctcgcaga gcacacactt tatgaatata aagtatagtg
      841 tgttatactt tacttggaag tggttgccgg aaagagcgaa aatgcctcac atttgtgcca
      901 cctaaaaagg agcgatttac atatgagtta tgcagtttgt agaatgcaaa aagtgaaatc
      961 agctggacta aaaggcatgc aatttcataa tcaaagagag cgaaaaagta gaacgaatga
     1021 tgatattgac catgagcgaa cacgtgaaaa ttatgatttg aaaaatgata aaaatattga
     1081 ttacaacgaa cgtgtcaaag aaattattga atcacaaaaa acaggtacaa gaaaaacgag
     1141 gaaagatgct gttcttgtaa atgagttgct agtaacatct gaccgagatt tttttgagca
     1201 actggatcca ggagaacaaa aacgattttt tgaggaaagt tataaattat tttccgaacg
     1261 atatggcaag caaaatattg cttatgcaac agttcataat gatgagcaaa cccctcacat
     1321 gcatttaggt gttgtgccta tgcgtgatgg aaaactgcaa ggaaaaaatg tgtttaatcg
     1381 tcaagaactg ttatggctac aagataaatt ccccgagcat atgaaaaaac agggttttga
     1441 gttgaagcgt ggtgaacgtg gctctgaccg taaacatatt gagacagcta aatttaaaaa
     1501 acaaactttg gaaaaagaga ttgattttct agaaaaaaat ttagcagtta aaaaagatga
     1561 atggactgct tatagcgata aagttaaatc agatttagaa gtaccagcga aacgacacat
     1621 gaaaagtgtt gaagtgccaa cgggtgaaaa gtccatgttt ggtttgggaa aagaaataat
     1681 gaaaacagaa aagaaaccaa ccaaaaatgt tgttatatcg gagcgtgatt ataaaaactt
     1741 agtgactgct gcgagagata acgataggtt aaaacagcat gttagaaatc tcatgagtac
     1801 tgatatggcg agagaatata aaaaattaag taaagaacat gggcaagtta aagaaaaata
     1861 tagtggtctt gtagagcgat ttaatgaaaa tgtaaatgat tataatgagt tgcttgaaga
     1921 aaacaagtct ttaaagtcta aaataagcga tttaaagcgt gatgtgagtt taatctatga
     1981 aagcactaag gaattcctta aggaacgtac agacggctta aaagccttta aaaacgtttt
     2041 taaggggttt gtagacaagg taaaggataa aacagcacaa ttccaagaaa aacacgattt
     2101 agaacctaaa aagaacgaat ttgaactaac tcataaccga gaggtaaaaa aagaacgaag
     2161 tcgagatcag ggaatgagtt tataaaataa aaaaagcacc tgaaaaggtg tctttttttg
     2221 atggttttga acttgttctt tcttatcttg atatatatag aaataacgtc atttttattt
     2281 tagttgctga aaggtgcgtt gaagtgttgg tatgtatgtg ttttaaagta ttgaaaaccc
     2341 ttaaaattgg ttgcacagaa aaaccccatc tgttaaagtt ataagtgacc aaacaaataa
     2401 ctaaatagat gggggtttct tttaatatta tgtgtcctaa tagtagcatt tattcagatg
     2461 aaaaatcaag ggttttagtg gacaagacaa aaagtggaaa agtgagacca tggagagaaa
     2521 agaaaatcgc taatgttgat tactttgaac ttctgcatat tcttgaattt aaaaaggctg
     2581 aaagagtaaa agattgtgct gaaatattag agtataaaca aaatcgtgaa acaggcgaaa
     2641 gaaagttgta tcgagtgtgg ttttgtaaat ccaggctttg tccaatgtgc aactggagga
     2701 gagcaatgaa acatggcatt cagtcacaaa aggttgttgc tgaagttatt aaacaaaagc
     2761 caacagttcg ttggttgttt ctcacattaa cagttaaaaa tgtttatgat ggcgaagaat
     2821 taaataagag tttgtcagat atggctcaaa gatttcgccg aatgatgcaa tataaaaaaa
     2881 ttaataaaaa tcttgttggt tttatgcgtg caacggaagt gacaataaat aataaagata
     2941 attcttataa tcagcacatg catgtattgg tatgtgtgga accaacttat tttaagaata
     3001 cagaaaacta cgtgaatcaa aaacaatgga ttcaattttg gaaaaaggca atgaaattag
     3061 actatgatcc aaatgtaaaa gttcaaatga ttcgaccgaa aaataaatat aaatcggata
     3121 tacaatcggc aattgacgaa actgcaaaat atcctgtaaa ggatacggat tttatgaccg
     3181 atgatgaaga aaagaatttg aaacgtttgt ctgatttgga ggaaggttta caccgtaaaa
     3241 ggttaatctc ctatggtggt ttgttaaaag aaatacataa a
//
LOCUS       DAXWOO010000212         3250 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.216, whole genome shotgun sequence.
ACCESSION   DAXWOO010000212 DAXWOO010000000
VERSION     DAXWOO010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.216"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(162..2159)
                     /gene="tkt"
                     /locus_tag="ACGE70_002683"
     CDS             complement(162..2159)
                     /gene="tkt"
                     /locus_tag="ACGE70_002683"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFF8694276.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2261..2512)
                     /locus_tag="ACGE70_002684"
     CDS             complement(2261..2512)
                     /locus_tag="ACGE70_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFF8694277.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2659..>3250
                     /gene="lexA"
                     /locus_tag="ACGE70_002685"
     CDS             2659..>3250
                     /gene="lexA"
                     /locus_tag="ACGE70_002685"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFF8694278.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGR"
BASE COUNT          885 a          720 c          608 g         1037 t
ORIGIN      
        1 ctcaagaaac acaaactaat ccgaagaatt ctttgttttg tcatttaaat gtaacatagc
       61 cgaaaacgaa aagtaatatt gatttattct tctaacacat aaagaacagc tgtctccaac
      121 ttgcagacag ctgttcttat cagtcaaaca attgcttgga tctatgatag ttgattgtac
      181 gtattcacga cattttctac tgtaaatcca aattcttcta aaattttagt gccaggggca
      241 gatgcaccga aatgatcgat tgtaatcgtt gcgccctctg ttcctacgta gcgttcccat
      301 ccaaatgatg cagcagcttc gatagccaca cgtttcttca ctgatttagg aagtacactt
      361 tctttatatt cagaagattg tttttcaaag aggtcaaagc ttggcataga aacaacagaa
      421 acatctttgc ctttttcagc caattctttt tgagctttca cagccaaatc aacttcagaa
      481 cctgttgcaa tcaaaatacc ttctggtgtt tctccttgtg atggagaaag aacatacgcg
      541 ccttttttca ccatttcatc tgctacttct tttgtagaag gcaatactgg aaggttttga
      601 cgactcaata caaggatagt aggcgcatct gttgtctcca tcgctacttt ccaagcagca
      661 cgtgtttcat ttccgtctgc cggacgaatc acttgaacac caggcatgca gcgaacagat
      721 gctagttgtt caatcggttc gtgcgttggt ccgtcttctc cgacagcaac cgagtcatgt
      781 gttaagacgt aaacaacagg agtatgttgg attgctgcta aacgaactgc tggtcttaga
      841 taatcgacaa agacaaagaa tgttccaccg tagatacgag ttccgccatg aagttggata
      901 ccattcatag ccgaagccat tgcaaattcg cggacaccga accagatgtt gcgtccttca
      961 taatgttcag gagtgaaatc tttatctgca gccaccattg tattattgga acctgacaag
     1021 tccgctgaac caccccagaa gcttggtata gctttcgata attcttggat gacttcttta
     1081 cttgaaacac ggcttgcttg actgcttcca acttcataag ttggcaattc tgcatcccag
     1141 ttttctggta gtttgccatc aaaagcatct tcaaattgtt gtgctaattc aggataagct
     1201 tttttataat tggcaaacat ttcattccat gcatcttcag ctttttgtcc ttcttctatc
     1261 atcgtttgat ggaagcgggc agctacttct tctggaacag tgaaatctgg atactcccaa
     1321 ccataaactt ctttagccat cttgatgcca tcttcaccaa gaggagcacc atgaacagca
     1381 gatgttcctt ctttaggagc accatagccg atgactgttt ttacttcgat caatgttggt
     1441 ttgtctgttt cagcttttgc ttcttcaatc gctttagaga ttgcttctag gtcatttccg
     1501 tctttcacta agatatgttg ccagccatat gcttcatagc gagcaccaac gttttctgtg
     1561 aaagcttttg aagttggacc gtctagtgaa atatcgtttg aatcgtataa aacgatcaat
     1621 ttccctagtt tcatatgacc tgccatcgaa cttgcttctt gagatacccc ttccatcaag
     1681 tctccgtcac cgcatattgc atacgtatag tgatccatca cattaaagtt ttctttgtta
     1741 tacgttgctg caaggtgcgc ttctgccatt gccataccga cagccattgc aatcccttgt
     1801 cctaatggcc ctgtagtggc ttctacacca tctgtatgat gtacttcagg atgtcctggt
     1861 gttttagaat cccattgacg gaattgtttc aaatcatcga ttgtcacttg atagccagct
     1921 aaatgaagca agctatacaa cattgctgac ccatgtcctg ccgaaagaac gaaacggtcg
     1981 cggtcagccc agttttttga tgtcgttgga tttactttca ggtgttttgt ccataacgca
     2041 tatgccatag gtgctgcgcc catcggcaat ccaggatgtc ctgagtttgc tttttggact
     2101 gcttcaatac ttaatgtacg aattgtgtta acgccaagtt ggtcaatttt gtcaaacaaa
     2161 ataacccctc ctattaattt aggttttttt tacctttatt tcttacactt tcattctata
     2221 agatgtcaca cgaaaaagca atctttttca gtatttttta ctaaaagttg tcatttcggt
     2281 tgtgtaagcc ttttttcttt tggatttctt ttaatttttc cggggtcacg tcattgccct
     2341 caggatcgac gactttcatt ccttcgatat gatgacgcat accggaacga aaagcttcca
     2401 aatactcttt gcgcaagttg atttgttctt gttcttcggc ttttgagaga ccattttcct
     2461 tctttttacg tgcaagttca ttgatccgct ctattttttc aggtgacagc atgctcagcc
     2521 ctccttttac tctccttcat attatataaa aactgaaaaa aaaacaacta gataaactaa
     2581 ataaaatacg aacattcgtt tgatttagcg ctttttatat ggtactattt atttatcaag
     2641 tgaaagaagg tgtaccttgt ggcccgacaa actgaatcaa gacaaatcga agtcttacga
     2701 tatatccatg aacaagtaac ccaaaaagga tacccgccga ctgtccgtga aatcggggaa
     2761 gctgttcagc tttcttctac ttctactgtt cacggtcatc ttgcacggct tgaaaaaaaa
     2821 gggtttatcc aaagagatcc gacgaaacct cgtgcaatcg agttgacaaa acaaggttta
     2881 gaaaagattg ggattcgtcc aacaactatt ccagtacttg gcgtcgtaac agctggagaa
     2941 cccattttag cagtagagga tgcttcagac ttctttccaa tccctcctaa tctttcttcc
     3001 gaagaaggca gtctctttat gctgacaatt cgtggagata gtatgatcaa tgctgggata
     3061 ttggatggtg acaacgtgat cgttcggaag caagaaactg ctcaaaatgg cgatattgtc
     3121 attgcaatga ctgccgagga cgaagcgact tgcaaacgat tctataaaga aaaaaacttt
     3181 ttccgtctgc agcctgaaaa cgacttgctc gaaccaatca tcttagatca agtatctata
     3241 ttaggacgag
//
LOCUS       DAXWOO010000213         3250 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.217, whole genome shotgun sequence.
ACCESSION   DAXWOO010000213 DAXWOO010000000
VERSION     DAXWOO010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.217"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(392..754)
                     /locus_tag="ACGE70_002686"
     CDS             complement(392..754)
                     /locus_tag="ACGE70_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFF8694279.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            977..1729
                     /locus_tag="ACGE70_002687"
     CDS             977..1729
                     /locus_tag="ACGE70_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFF8694280.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(1869..2792)
                     /locus_tag="ACGE70_002688"
     CDS             complement(1869..2792)
                     /locus_tag="ACGE70_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8694281.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(2807..3079)
                     /locus_tag="ACGE70_002689"
     CDS             complement(2807..3079)
                     /locus_tag="ACGE70_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694282.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         1068 a          611 c          529 g         1042 t
ORIGIN      
        1 gataaaataa gtagattttc ctactacaat cgtagccgtt aagataccga tgattgaaca
       61 aatataacca aatgtctcta caacataatc aatggcaaaa gtttttgaag caataatacg
      121 tctttccttt tttgcttctt atccccaaag aaacggatat aacttattat gtattgaatt
      181 tttttggaaa ggcgaattat gacaagatat accttgcgtt gaaaaaactc gcggaaaaaa
      241 tatcacacgt ttttaaaaag atacaaagta agaaagcagc tgtcaataga atggaaacaa
      301 aaatggcgag acatcaatca gtctcgcctt gttttttatt gtatacatta gatatggagc
      361 aggttaccat aaagatgatt attagtcgtt attaaataga atccttcgta tttttttgtt
      421 ctatcactac aatatcttta tttggacgtt tgaattgtat tccggcacgt tctagctcct
      481 gtttcactcc tacatggact ttgacatctt ctcgtgtcat ttttaaaacg tgatgaatcg
      541 catagtcgat ttcttgcctt gtgatttttt tcttcgtatt gatcatcacg acttgctttt
      601 tatcatatgt gtctaccaat agcagttgtg ttcgatctaa cgaaaataat ttgtaataat
      661 taatagccgt attcccaaaa aagaattcca ctactttagg ataaaatgtc ttaatattta
      721 aattactatt cacaggtgtt tcgattagct tcatctcatt cctcttttct atatagtcga
      781 tttagatcca atcttaagtt atgttaccat ctttcctagc attaaggaat cggttacaat
      841 tgaaccttta aagacaaaag gatttacctt ttatcactta atagtataac ataatttcgt
      901 gagaaaaaaa tacaattttt tcttgaccat ctcgttacgt aaaggattat gcttacattt
      961 gtcaggagga aaaataatgg agtacacaat aaagaaaatg tcagagatat caggagtcag
     1021 tccgagaacc ttgcgctttt atgatgagat tggactgcta aaaccagcta ggatcaattc
     1081 ttcaggttat cggatttatg gcaaaaaaga agtcgatcgt ctgcaacaca tcttatttta
     1141 tcgaacaatg gcattcaagt tggatgatat ccaagaggtc ttagacaatc catcttttga
     1201 ccaccagaaa gctttaatca agcatcagca aatgttgctg gaaaaacggg cacaaatcga
     1261 taccctcttg acaactgtcc aacagacact tgatatgtac gaaggaggaa gaaaaatgag
     1321 tgacaaagaa aaatttgaag ggtttaaaca acaaaagttg aaagaaaatg aagaatccta
     1381 tggacaagag attcgacaaa aatacggaaa aaaaaaggtt acagcatcca atgaaaaatg
     1441 gatgaatatg gataaagaaa cgtatgaaga aatgcaaact gttgaaaagc agatgttatt
     1501 tgatctcact ctttacttaa aagaaccaga agatgaaagc ttagcagacc gtatcttcca
     1561 agctcataag aagtggttct cttacagctg gcctgaatat cagccaaaag cgcataaagg
     1621 actaggaatg atgtacgaag ccgatgaacg gttcactgca tattatgacg agcgaagtgg
     1681 tcatggtgca gcaaaagctt taaatgaaat tatccaaaaa aaggcgtaat cttttacatg
     1741 atccaacaaa aaagtacacg taaaatgacc taataacaaa acagcagaca cccaagttag
     1801 atttttatgc taacttttgg tgtctgctcc attctttcac aagttttttc atcctacttt
     1861 aaatatattc atcgttgaat aaacgtataa ggtattttca ctttatcctt tttttctgta
     1921 agaaacaatt gaaatgccat ttctccgcaa gcttccagat ggtggtcgat ggttgggaaa
     1981 ccaagaagct cgctgatcag caaattatcg cgtccaatca ccactggtgt cttatcttgc
     2041 agatagcttt gcaagatgcc tgctgcaacc atatccgaat ttgtaaagac accatcaact
     2101 ccaagctctt taaaaaagaa gcctgcttgt tttcctttct ctgcatctat acaatcccaa
     2161 aaaacaagtg attcatcaaa agatggaaaa agttgatgac atagctgcaa agtgattttt
     2221 gagttataac ttagcctgcc acttcgcccc aacgtgatgc ccaattgctt tataccttgc
     2281 tgcttcaaat aagtaagacc ttctgtaatt gattgttctc gatcaataaa gacacagctt
     2341 gcctgttttc ccttgatttc ttcacaaaaa actagaggtc catatttttg atatgccaat
     2401 aaatcatcta gtgtattggc acgagacgta atgatcaatc catcgaaagc tttagctgca
     2461 aattcttcaa gataccttcg ctctttttct ttatgatagc ccgtcggtaa aagtgtcact
     2521 ttgtaatcat aatcaaacgc tgcttcaatg atcccactga tcaactgatc aaaatacgga
     2581 tgattggtat aaggcagaat cactccaatg cttttcgttt ttccataact taactgtctg
     2641 gcatttgcat tagggatata atcgacttct ttcatgaccg caagtatttc tttccttttt
     2701 tcctcatcta catatggatg tccattgatt acacgtgaaa tagtagaaac tgaataacct
     2761 gtgagctttg cgatatctcg aatgtttgcc actgctgctt cgccacctat acaaaaagat
     2821 tttcttgtaa atagtctacc acagctggac cgcattcaaa aaactgatac atttttcctt
     2881 catgcgaaaa actgatccgc tgcccgcccc aatatatatc ttttgtcaaa ttttcgattt
     2941 cattcattgg tatttctttt actatatttc tattcaaccc ttgaaaactg tacaacgtta
     3001 atacggatcc cttgaactcg acaaacgcat aaaaataatt gttcgttgtg ccatctgtta
     3061 tctctacata agtattcatt gcaagccctc cttttgatac tttgagtata gtgtatgaaa
     3121 cgcgtctcat agcaaggagt ttgttttgcc aaagaaaagt tttattcctc tttcattatt
     3181 accaaagtct cttattttaa attgaataca ttataatgat actgtaaata tatctttatt
     3241 attagtgaat
//
LOCUS       DAXWOO010000214         3250 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.218, whole genome shotgun sequence.
ACCESSION   DAXWOO010000214 DAXWOO010000000
VERSION     DAXWOO010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.218"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(54..126)
                     /locus_tag="ACGE70_002690"
     tRNA            complement(54..126)
                     /locus_tag="ACGE70_002690"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(91..93),aa:Gln,seq:ctg)
     gene            complement(172..600)
                     /locus_tag="ACGE70_002691"
     CDS             complement(172..600)
                     /locus_tag="ACGE70_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694283.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(660..1418)
                     /locus_tag="ACGE70_002692"
     CDS             complement(660..1418)
                     /locus_tag="ACGE70_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFF8694284.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            1713..1910
                     /locus_tag="ACGE70_002693"
     CDS             1713..1910
                     /locus_tag="ACGE70_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694285.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            2016..2498
                     /locus_tag="ACGE70_002694"
     CDS             2016..2498
                     /locus_tag="ACGE70_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694286.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(2556..3152)
                     /locus_tag="ACGE70_002695"
     CDS             complement(2556..3152)
                     /locus_tag="ACGE70_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFF8694287.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
BASE COUNT          968 a          545 c          636 g         1101 t
ORIGIN      
        1 gcaaaaagat tgttctttta cagggggttc attttttcag caagcacatc aaattgggat
       61 ggcagggttc gaacctacaa ttgcttgatt cagagtcaag tgccttacca gtttggctac
      121 atcccattta ttcaacaaaa taatcatagc actttcctaa aaaaacgcaa gttatttttc
      181 ttgatcggat agttgttgct ttactcgtaa aaggcggcgt actagtttct tttctaatcc
      241 agcaggaagg gcaatcagtt ctgctactga caagacggtg tccaataact gatattcttt
      301 gacaggttca ttcataatat gatagatgtt tgctaaacgt tcaatatatt caaagcgagt
      361 atactctgtt cgagcaatta gaaatttcaa taaatcggtg gctttctcgt actcttgatc
      421 tgcgtaataa acaagagatt gacgatacaa tttgatttcc agttcattga gggtagcagg
      481 tctgcgacgc tcgttccact cattaataga gactttacct tgaacatttc ccttgcagtc
      541 acggacaaaa ataatcggaa tttcatttgg atccattttt ttatgtgtga atttttccat
      601 tatctacgct cccttcaaag gtttggtatc ttgagtataa gggatttttg tttgaattac
      661 tagcttttct ccaaagttag catctgtcca cccattgcag tgaaaatcgc cgaaaaatcg
      721 ttgatcggta ctgctcgatt ttttacacca tagggatcgt tgacataaat attttctttg
      781 tcatatccag tgactacaca agcatgaaca agaggagtga ctcttacttc tccattattt
      841 gtggtccaag tcataaaatc agtttcatct gggacttcaa aattgatagt cacagaagtc
      901 cagacaggat ttcctttaga tagtgctttt ttgatatcat cgagttgagc atcggttttt
      961 ggaaaagtgt gataatccgt ttggacgatt tgctgagcaa gggaggcaac tgggggaaca
     1021 tagacgacca tagcccagtc tccgccactg atatcaccaa caaatccttc attaggatcg
     1081 ccatgagtcc catctgcatt gaaaacaggg acgtaatcca attgctcagc taactggttt
     1141 ttagttgttt cataaccata aaaagataaa agcatagaaa gcgaagtgac ttcacagcca
     1201 ttttctaaag aaggagaatc aaactggctc tctaatggaa catctagtaa aacttctgct
     1261 ttctcttttg tctttttcgt tttttgtgtt gaactttttt gtgcagtgtt cattgtatgt
     1321 tctggagaat ggatatgtag gtaataactt gtaccgctta ataaaagagc agctgtgccc
     1381 cctagaaaca atagtttttt gaacgttttt ttcttcattt gaaatctccc ttttcagctg
     1441 tttatctttc ctattataaa tgtattttta ttggaaatgg aagtattata ttttaatatg
     1501 taggaaataa ttagttttta aggaatatgt tttaattttt tgaaaaaaat tcttctaaat
     1561 acaacagctg ttttaattgt gaaaacgaaa acagtacgct ataatgcctg taaggttgat
     1621 ttttaactgt aaataaaaat caatgagtta aatctccaaa gaaatagcag tggtaatgtg
     1681 gagaaaagtt ccagcagttt aggaggattt tgatgacgga agaaatgatt aatttaggag
     1741 aacaatatgc atgcaaacca attggtttta caaaaacggt cattggtgaa gttgtatcaa
     1801 aaatgacaaa ctgcgccgtg gtcaaagttg cccaatgtgc tgcagaggac caagaattat
     1861 tagatgaaaa agcaagcatg gttgttgcaa aatatgatac atttgaataa agaaaacaat
     1921 tggacgtgcg gcgtgctgca agtccaattt ctttgagaag gtaaatcagt attcttttcc
     1981 tattttctgg agaagaaaaa gcgaggcgag ttgtcatggt aacattcttt acggttctat
     2041 tgtttttatt cgttgttgtg atgtttttat ttctttggct ggttcgaaaa gaaattattt
     2101 atcggactgt gcgaaatcga tgggtctact tggtgatacc cttcttagtg tcactagtca
     2161 tttggtatac actcatcagt catcctacag cagatgaatt ggcaaaaggg atcttgtcag
     2221 caatgatttt tattagtttt ttgttggaca gtagaggaat cacagaagaa ggacttgttt
     2281 tgaatagctt tgacaaaaaa ggtgttcctt tttctgagat caataaaatt gtattatatc
     2341 agccaaaagg ttctaaaatc gtaaaaatga attttttccg aaacggatgg cgaggaccga
     2401 tgctgaaatt ttctgcatca ttagaagaat tagttccatt tttgtcccaa cgcttaaacg
     2461 atgaggcaga gattgatatt atgattgatc cagaataaaa acagaggttg cgacaagatg
     2521 tttgtcacaa cctctgtttt ttatttcaaa taaaattatc caaatacgcg gaagcgatcg
     2581 gcatcgttca tgtattcttt ttccccagcc ggtgtttctg gtgaaccgaa tactaattgc
     2641 gaacgtagtt tccagtttga agggatattc cattcttttg caactgcttc gtcaataact
     2701 gggttgtaat gttgcaagtt tgcgcccaat cctttatcaa ctaatgctac ccaagtatta
     2761 gctgttgcga ttccgtttga ttgttctgac caatctggga agttgtctgc atataattca
     2821 aattgttctt gtagaccttt taccacgtca gtatctttga agaataatac tgtaccataa
     2881 ccatttttga aaccagctaa tttgttttgt gtatttggga aagcttctgc tggagtcaat
     2941 gggcgaagtg cttcttcagt gatttcccaa agtttttcat gtgcatctcc gaaaaggata
     3001 actgcgcgtg ttgattgagc attgaaagca gttgggcttt ctttaattgc ttctttgatc
     3061 aaagttgtta attcttcatt agaaagtgaa acattacgtc ctagatgata gattgaacgg
     3121 cggtttttca aagtgtcagt aaaagttgtc ataataaaat ttctcctttg atttgcgttt
     3181 tgcatttttt taattgatta cgaaatgaag tatagcacct tacaaaaagt aagtaaacag
     3241 atatgctcac
//
LOCUS       DAXWOO010000215         3226 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.219, whole genome shotgun sequence.
ACCESSION   DAXWOO010000215 DAXWOO010000000
VERSION     DAXWOO010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.219"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(29..973)
                     /locus_tag="ACGE70_002696"
     CDS             complement(29..973)
                     /locus_tag="ACGE70_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFF8694288.1"
                     /translation="MDTILITGGAGFTGSTLANYLGKENKIVVVDDLSMGKKENLDMD
                     KQVTFIEGDVSDSQLMERIMKEYQFAYIFHLAAVASVADSVERPVETHRVNFDSALLL
                     LELARKYQSNLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFAAEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKAEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILSGMERYLKTEI"
     gene            complement(1107..2498)
                     /locus_tag="ACGE70_002697"
     CDS             complement(1107..2498)
                     /locus_tag="ACGE70_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFF8694289.1"
                     /translation="MLNERFLKYVKGWYFIYFGFILMLRAIGPYLLLPTRYDTLLFWP
                     AGIAGVVLIIIDAIIAFREKNLKKYDWLLIAFIGAMLVSSLMNLKYGYSENIKLIMWQ
                     LIFFFMIYQFGKDYSSNFFFKVFTWILSLVWFLANLVSLYWFVIQYGIKIPIKYKYYP
                     VRLGWLGNRLFGVYTDPNFGAVISLIVIIIAIYYLVTYKHLNLLFKIFFVINIFIQFS
                     FIALSGSRTTLVCLLITTFVFSFFTSYSSRFLKTNTLKRWLTALVVGLLSIIAMYYVT
                     DITAHAWAHVPHITEVKKDTSDSSGTTSSNKVDLKRPDTDSGDVSNLRFELWKSAIEI
                     FKSKPISGGSPGYYIEYAHDKLPYTLMGKDTLTAHNFIFLVMAATGGLGLLTLLTFLF
                     IQCFKALRYCFTQPNLVKDKHFFAILMILTIAVSALFITELILVSTIGSFIFWGYLGS
                     IQKTISKEEGVTN"
     gene            2624..3034
                     /gene="tagD"
                     /locus_tag="ACGE70_002698"
     CDS             2624..3034
                     /gene="tagD"
                     /locus_tag="ACGE70_002698"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047348 - glycerol-3-phosphate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HFF8694290.1"
                     /translation="MKRVITYGTFDLLHYGHINLLKRAKQYGDYLIVALSTDEFNWNA
                     KQKKCYFSYEKRKQLLEAIRYVDLVIPEENWEQKVKDIKEYHADYFVMGDDWAGHFDE
                     LEQLTDAQVIYLPRTPEISTTQIKNELNSLNSDK"
BASE COUNT         1157 a          563 c          457 g         1049 t
ORIGIN      
        1 tttatccaaa gatgcaatca ttttttcatc aaatttctgt ctttaaatac cgttccattc
       61 cgctcaatat atcaaattta ggctgatagc ctaaagaacg aatttttgaa atatccgcta
      121 aggaatcacg aatatcccct gaacgctccg cttcatattt aagcgctagc tctgttccta
      181 agatttgatc aattgaatgg atcaattcaa gtagcgtagt gctttttcct gtaccgacat
      241 tgaattgctg tcctaaagct ttttcttcct tggcaacaag taaaagtgct tgtataacat
      301 cttctatata aacaaagtct ctagactgac tcccatcgcc aaataaagta aattccgctt
      361 tttctcctgc caattgtttt ttgtaacgat ccactagaat cgaaatgact ccgctatatg
      421 gagagtttgg attttgattt ggaccataaa cgttaaaaaa tcggacagca ctagtcggaa
      481 catcatataa atgacagtaa tccaaaacat attgttctgc tgcaaattta tcgatagcat
      541 atggtgttaa tggacggatg actgattctt ctttttttgg cagtgttggt tcatcaccat
      601 aaactgcagc tgatgaacta aatactaatc gttttaaatt agattgatat ttccgtgcta
      661 actcaagcaa taaaagcgca ctgtcaaaat ttacacgatg tgtttctaca ggccgttcta
      721 cagaatctgc cacactagct acagcagcta gatgaaaaat ataggcaaac tgatattctt
      781 tcatgattct ttccatcaat tgagaatctg atacatcccc ctcaatgaaa gtcacttgtt
      841 tatccatatc caagttttct ttttttccca ttgataaatc gtcaactact acgattttgt
      901 tttcttttcc caaataattc gctagcgttg aaccggtaaa accagctccg ccagtaatca
      961 atatagtgtc catcaaattc actccaattg cacattttcg tatcaaataa tattagttta
     1021 ccatttttat attgtaaaaa ctataaaatt tgttgaagtt gggataacta gttttctttt
     1081 ctactttcat atacctaaaa atagtttcaa ttagttactc cttcttcttt agatatagtt
     1141 ttttggatag aacccagata tccccaaaaa ataaatgaac ctatcgtact aaccaaaatg
     1201 agttctgtga taaataatgc cgaaactgct attgtaagaa tcattaaaat agcaaaaaag
     1261 tgcttatctt ttactagatt tggttgtgtg aaacaataac gcaaggcttt aaagcattgg
     1321 ataaataaga aggttaacaa tgtcaataga cctaatcctc ctgtagcagc catcactaag
     1381 aaaataaaat tatgagcagt taaagtatct tttcccatta atgtatatgg tagtttatca
     1441 tgtgcatatt caatatagta gcctggagaa cctccggata tcggcttact cttgaatatt
     1501 tcaattgcac ttttccaaag ttcaaacctt aaattagaaa catccccgct atcagtgtca
     1561 gggcgtttta aatcaacttt attgctgcta gttgttcctg atgaatcaga tgtatctttt
     1621 tttacttctg taatatgcgg aacatgtgcc catgcatgag cagttatatc tgttacataa
     1681 tacatcgcaa ttatacttag caatcctact actaatgcag ttagccatct ttttaaagta
     1741 ttagttttaa gaaaacgact actataactt gtaaagaaac taaaaacaaa agttgttatt
     1801 aataaacaaa ctaatgtggt gcgtgaaccc gataaagcaa taaacgagaa ttgaataaaa
     1861 atattaatta caaaaaatat tttaaataga agatttaaat gcttgtaagt gaccaaataa
     1921 tatatagcaa taatgatgac gattaatgat ataaccgcac caaaatttgg atctgtatat
     1981 acaccaaata aacggttgcc taaccaccct aaacgaactg ggtaatactt atattttatt
     2041 ggaattttta tgccatattg aatgacaaac cagtataatg atactaaatt agctaaaaac
     2101 cataccaatg aaagtatcca ggtaaatact ttgaaaaaaa agttactact atagtctttg
     2161 ccaaactgat aaatcataaa gaaaaagatt aattgccaca tgattaactt aatattttcg
     2221 ctatatccat attttaaatt cattaaactg cttactagca tcgcgccaat aaaagcaatt
     2281 aatagccaat catatttttt tagatttttt tctctaaatg caataattgc atctataatg
     2341 atcaagacaa ctcccgctat tccagcaggc caaaaaagta aagtatcata tctagtaggt
     2401 aatagtaaat acgggcctat tgctcgtaac attaaaataa aaccaaaata aataaaatac
     2461 caacctttca catattttaa aaatctttca ttcaacattt ttcctccatc cacctatttc
     2521 tcataaatat ttcttatcct ttttaggata aactggtcct ttcaattcta acaaaaaaat
     2581 gctaaactaa caatgtatat taatgaattt cgagaggtga aagatgaaaa gagtaattac
     2641 ttacggtacc tttgatttgc ttcattacgg gcatatcaat ttactgaaac gagcaaaaca
     2701 atacggagac tatcttattg ttgccctttc tacagatgaa ttcaattgga atgcgaaaca
     2761 aaaaaaatgt tattttagtt atgaaaaaag aaaacagtta cttgaagcta ttcgctacgt
     2821 tgatttagtt attcctgaag aaaattggga acaaaaagta aaagatataa aagaatacca
     2881 tgcagattat ttcgtgatgg gcgatgattg ggctggacat tttgatgaac ttgaacaact
     2941 aacagatgca caagtgattt atttaccaag aacacctgag atctctacaa ctcaaataaa
     3001 gaacgaactt aattctttaa atagtgataa ataatatttt ttaaacaata aaagaacaat
     3061 atatctggat aagacgtaaa aaaatggctc tataatttct aggactgata gaattttctg
     3121 tcggtcctag attttttgta aaaaaaaaca gacataaaaa aattccgata gaatagagtc
     3181 gtctaaaaca tcaatctgaa aggaagtttt tgatatccat ttctca
//
LOCUS       DAXWOO010000216         3218 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.220, whole genome shotgun sequence.
ACCESSION   DAXWOO010000216 DAXWOO010000000
VERSION     DAXWOO010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.220"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..1438
                     /locus_tag="ACGE70_002699"
     CDS             <1..1438
                     /locus_tag="ACGE70_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HFF8694291.1"
                     /translation="SKKNQPKDIKDIVDTRMYKERDDLNYTLIINKDGNYSWRPLVLL
                     HPILYVDLVNCLTKEDHWEELKKRFSEFKKDKNINCYSIPVEASKGSKRTDLGETILN
                     WWEYFEQKSLAQNIKYQYCMFTDIADCYPSIYTHSITWAMYGKEKVKNDENKCSGSLG
                     DKIDKKISGMQHNQTNGIPQGSVLMDFVAEIVLGYVDLRLSKELKKLENLKDYKVLRY
                     RDDYRIFSNNRNDLEQIMKVLSEVLYDLNFKLNSSKTKLSDDIIIDSIKKDKLYWEEI
                     KASIRMNATNLDGDREIRYSINLQKHLLEIKKLADKHPNSGQLKKALSEFNKERIIKI
                     TSKPNDLLPLIGILVDIMINNPGSLPQCVLIIGQLLNFENESKAREIVEMIINKYALK
                     PNTEFMMIWFQRLTTIFGEMFDSVLSSEFVSKIKDPVSNQMFSVDWLNEDAKKSFYEG
                     TIKDEEKYIKMSKKIYITEFDDFNDYPF"
     gene            1776..>3218
                     /locus_tag="ACGE70_002700"
     CDS             1776..>3218
                     /locus_tag="ACGE70_002700"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFF8694292.1"
                     /translation="MTEQIEKNSKSLYQALWNSADILRSKMDANEYKSYLLGLVFYKY
                     LSDNMLRYVSVLLEEETDDLNKAQDLYVEAFKDADIKDDLLYELKDEFSYTIAPALTF
                     TAQVAAIHDGSFQLEDLVQGFRDIEQSSENFENLFEDIDLYSKKLGSTPQKQNKTIAD
                     VMKELEGLNMAGHAGDVLGDAYEYLIGQFASESGKKAGEFYTPQPVAKLMTQIVLQGK
                     EDKKGFSVYDATMGSGSLLLNAKKYSHQPGTISYFGQELNTSTYNLARMNMILHGVPI
                     ANQHLHNADTLDQDWPTEEPTNFDGVLMNPPYSAKWSADKGFLDDPRFSAYGVLAPKS
                     KADFAFLLHGYYHLKDTGVMAIVLPHGVLFRGGAEGKIRKALLENGAIDTVIGLPANI
                     FFNTSIPTTVIILKKDRAKKDVLFIDASQDFEKIKTQNTLRDYHIDAILEAYKTRTIK
                     DKYAYVAEFDEIVENDYNLNIPRYVDTFEEE"
BASE COUNT         1183 a          505 c          582 g          948 t
ORIGIN      
        1 ttcaaaaaaa aaccaaccga aagatataaa agatattgta gatacacgaa tgtataaaga
       61 acgtgatgac ttaaactata cgcttattat caataaagat ggaaactatt catggcgtcc
      121 tctcgttcta ctacatccta ttctatatgt agatttggtt aattgtctca ctaaagaaga
      181 ccattgggag gagttaaaaa agaggtttag tgaatttaaa aaagataaaa atattaactg
      241 ttattctata cctgttgaag cttccaaagg ttcaaaaagg acagatcttg gtgaaacaat
      301 actaaactgg tgggaatatt ttgaacaaaa aagcttagct cagaatatta aataccaata
      361 ttgcatgttt actgacatcg ctgattgtta tccatcaatt tatactcact ctataacatg
      421 ggccatgtat ggtaaagaaa aagttaaaaa tgatgaaaat aagtgttcgg gctcacttgg
      481 cgataaaata gataaaaaaa tatctggaat gcaacacaat caaacaaatg gtatccctca
      541 gggttccgtt cttatggatt ttgtagcaga gatcgttcta gggtatgttg atttgagatt
      601 atcgaaagaa ttaaaaaaat tagaaaattt aaaagattac aaagtactca gatatagaga
      661 tgattataga atattttcaa ataatcgtaa tgatttggaa caaattatga aagtgttatc
      721 agaagtctta tatgatttaa attttaagtt aaattctagt aaaacaaaat tatcagatga
      781 tattatcata gattctatca aaaaagataa gctctattgg gaagaaatta aggcaagtat
      841 aagaatgaat gccacaaact tagatggtga tagagaaatt cgttatagta taaatttaca
      901 aaaacattta ttggaaataa aaaagttagc agataagcat cctaattcag gccaattgaa
      961 aaaagcactt tctgagttta ataaagaacg cattattaaa atcacttcaa agccgaacga
     1021 tcttttacca cttattggta tcctggtaga tatcatgata aataacccgg gttctttgcc
     1081 gcagtgtgtc ttaattatag ggcagttatt aaatttcgaa aatgagagta aggctagaga
     1141 aatagttgaa atgattataa acaaatatgc tttaaagcca aatactgaat ttatgatgat
     1201 atggtttcaa agattgacca ccatttttgg agaaatgttt gatagtgttt tatcatccga
     1261 atttgtatct aaaatcaaag atcctgtctc taatcagatg ttttctgtag actggctaaa
     1321 tgaagatgct aagaaatctt tttatgaagg gaccattaaa gacgaagaga aatatattaa
     1381 aatgagtaag aaaatatata ttactgaatt tgatgatttt aatgattatc ctttttaagt
     1441 gaaaatttag aatgaatata aaaaacatta caatctaatt tttttaaaaa aatgttatat
     1501 cggaagtaat ctcttgattt ctatactata agataagaaa accactattt aacaaggtta
     1561 ttcgaacctt attttaagtt acttattatt taatgtatcg aaaaaggtga aataggtttg
     1621 ttataataag aattaagtaa gacatgaatt gtctaagcaa atgtagctta tccatttcat
     1681 taaaatacat atggtgaaaa aacaagttca aatagtaact aattttttaa aaaactgatt
     1741 tataaaaaat acaacacgaa tgaggaaaat aaccaatgac agaacaaatc gaaaaaaatt
     1801 caaaaagctt atatcaggca ctgtggaatt ccgcagatat cctgcgttca aaaatggacg
     1861 ctaatgaata caagagctat ttactaggct tggtctttta taaatactta tccgacaaca
     1921 tgctgcggta tgtctccgtt ctattggagg aagaaacgga cgacctaaat aaagcacaag
     1981 acctttacgt tgaagcattt aaagatgcag atataaagga tgatctcttg tatgaactaa
     2041 aagatgagtt ttcatatacc atcgcacccg cattgacctt taccgcgcaa gtggcagcca
     2101 ttcatgatgg atcttttcaa ttagaagacc tggtacaagg tttccgtgac attgaacaat
     2161 ccagcgaaaa ctttgagaac ttatttgaag acattgatct gtattctaaa aaactggggt
     2221 caaccccaca aaaacaaaat aaaaccatcg cagatgttat gaaagaatta gaagggttaa
     2281 acatggcggg gcatgctggc gatgtcttag gcgatgccta tgagtactta attggccaat
     2341 tcgcatctga atccggtaaa aaagccggtg aattctatac cccacaaccg gtcgcaaaat
     2401 tgatgacaca aattgtttta caaggaaaag aagataaaaa aggcttttct gtttacgacg
     2461 cgaccatggg ttcgggctcg ttacttttga acgctaaaaa atattcccat caaccgggaa
     2521 ccatttctta tttcggacaa gagttgaata cgtctaccta taatttggcg cgtatgaaca
     2581 tgattttgca tggggttccc attgccaacc aacacttgca taatgcggat accttggatc
     2641 aagactggcc aacagaagag ccaacgaact tcgatggggt gctcatgaac ccaccgtatt
     2701 ctgcaaaatg gtcggctgat aaaggcttct tagatgaccc acgcttctct gcctatggcg
     2761 tattagcccc taaatcaaaa gcggactttg ctttcttatt acatggctac tatcatttga
     2821 aagatacggg cgtcatggcc attgttttgc cacatggggt gctctttaga gggggcgcag
     2881 aagggaagat acgcaaagcc ttgttagaaa atggtgccat tgacaccgtc attggcttac
     2941 ctgctaacat cttctttaat acgtccattc caacgaccgt aattatcttg aagaaagacc
     3001 gtgcgaaaaa agatgtgctc tttattgatg cgtcacaaga ttttgaaaaa ataaaaacgc
     3061 aaaatacgct acgagattac catattgacg ctattttaga ggcctataaa acccgtacaa
     3121 ttaaagacaa gtatgcttat gtcgcagaat ttgatgaaat tgtggagaat gattacaact
     3181 taaacattcc ccgttacgtg gatacctttg aagaagaa
//
LOCUS       DAXWOO010000217         3204 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.221, whole genome shotgun sequence.
ACCESSION   DAXWOO010000217 DAXWOO010000000
VERSION     DAXWOO010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3204
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.221"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            679..765
                     /locus_tag="ACGE70_002701"
     CDS             679..765
                     /locus_tag="ACGE70_002701"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFF8694293.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(1067..1291)
                     /locus_tag="ACGE70_002702"
     CDS             complement(1067..1291)
                     /locus_tag="ACGE70_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694294.1"
                     /translation="MEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNMTPERKRS
                     LEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
     gene            1657..1941
                     /locus_tag="ACGE70_002703"
     CDS             1657..1941
                     /locus_tag="ACGE70_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694295.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            1944..2360
                     /locus_tag="ACGE70_002704"
     CDS             1944..2360
                     /locus_tag="ACGE70_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694296.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(2497..>3204)
                     /locus_tag="ACGE70_002705"
     CDS             complement(2497..>3204)
                     /locus_tag="ACGE70_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFF8694297.1"
                     /translation="TELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLKKVRF
                     SFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEINKTK
                     KFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYYVVRI
                     EEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLEFTFS
                     SLTSDLPKISLRNWLEE"
BASE COUNT         1065 a          599 c          500 g         1040 t
ORIGIN      
        1 tccgtttttt catccattcc tttcttcccc ccttacaaat aaataataac ctttaacaac
       61 caactcgtaa ataggttggt tgttattata caaaaggata ttagtactca gataaaaagt
      121 tgttagtact cagataaaaa gttgttagta ctcagataaa aagttgttag tactcagata
      181 aaaagttgtt agtacccttc tgtatccgtt gggagagtaa ggctcaactg ccccctaaaa
      241 agggttctaa aaaatcttta aaaaataaat aaaataaacg cgcgcgcgcg agcaaaattt
      301 cccgccaaaa aatcacccac tttttaaaat aaacaggttc gcgcatgata ttaaactgcc
      361 aatctagctc cctatggtcg ctgtaagaag gagtgatcgc tacgctctca cattttactt
      421 gcagcagtcg cctttaggct ccttcgccaa aaataattca ttagggactc ctttgtggcg
      481 tcctgagagc tcgtctaagc cgttctaaga caagattcga taaaaactat gtccttcgtt
      541 ctaaagcgcc ttacaggcgg tcagggcaag ccctaacaac cccaaggcaa taaaaaactt
      601 aggcttattt ttgctttttt attgtataat tgattgtcgc ctatccgaca gtcttgcgac
      661 tggaaggagg tgtgtttcgt gttcactaac tttatttgct cccttcttgt tgggctttta
      721 agcgctttgt tcagttactg gttgaataac cgaaacaaga agtaaaactt aattaaggcg
      781 actcagccca caaaaaaagc cccagagaat ccgacctctc tggggctttc gtgtgtttcg
      841 tgttcactta tttgcaatca aattataaca gaaataaata tttatttcaa aaatatccta
      901 caaaagtcta aaatccgtaa ttatttctta tatgcaacat ttacaaataa agtcgctctg
      961 agagctcgta tttttcgatt taagacgttt tatttagttt ttggtgtatt tcattggtcc
     1021 aagctaaaaa actcttagaa gcgattttag agccatttaa acccacttaa ttatattctc
     1081 catgaaattc gctccatgcg tcaaaagctg cgtctgaaat tccgccgtct tcgttcacta
     1141 gttgcattaa tttttccaac gatcgttttc tttcgggtgt catattttta tattccaaca
     1201 atttttcgtc tgttaaatcg aacttgattg cttgaattgc ttgctctaat ttttctaagt
     1261 tttgaaaata ttcgtaagga aatttttcca ttttttcttt ctccagtctc acaatttttc
     1321 tagaaataaa atcacttctt tgccgtctat actctcaaaa aaaatgtcgt ttgaaaaact
     1381 aaataaattt ttgatcgtgc atttttaaat ttggttatcg attacacgtg cagcactccc
     1441 ccccgaattt taaagagcat aaggaaacgt gtacccactt tctaaaaaaa gtgggtagat
     1501 aggctgtgcc tatcacgttt ctcagaaaaa aatttgatat cactttttcg ccatcgtggg
     1561 tagcttatgt atagcatttt ggcaaaaaac agctatacaa atatagtatt tgtatggtat
     1621 acataaatta tacataattg caaaaggaga ttttttatgg acattttagt tcggaatatt
     1681 gatccaaagt ttgttgcaac aatagacaaa cgttgccaag aactcacgaa taaaacagga
     1741 gaaaaatgga gtcgaaataa atatctaaaa gttttaatcg aaagtgattt tgaccgtcaa
     1801 cttacgactt acaaaaaaga ccaattcgat ttattgctcg ataaatttat tgctatccaa
     1861 acagaaaata ctcaaatatt aagcgagtat gtctacacaa ataatcaaat cattgaaatg
     1921 cttatcggag gaatgatata accatgagaa aaacttctgt ccaaaaacat gttactttgt
     1981 accctagtca tgttgacgtt ttagattcca tcatcgaaaa aaatccagat atcaataact
     2041 actcggaagc cattcgcttt ttatgtatga gttatgaacc cgataccaac acgaccacta
     2101 ccagcaatca ggcaaaacta aatgctatgg gaaaagaaat ttccatcatg actgaaatca
     2161 tcgcagaatt tgctgattta tatctaaaag atacaggcgt tcttattggc acaaattcta
     2221 aagtatatca agaagtgaaa gaaaaaatcg aagctaaact aaatccatca aatcgaaatc
     2281 gaccaaagaa tcatcaaaaa gaaaccagtc aaacagtcac ttctagaaat ttcgacccaa
     2341 caaattttct aaaacgataa aaaacagata ggagttattc ctatctgttt tttatttaga
     2401 agccccatac aatatgctag aatgtactag caatcttagt cggttgtaag ggacttgtct
     2461 acttgcttaa tgtaaggggg gcgagttctt ttttatttat tcttctaacc agttacgcag
     2521 agaaatcttt ggcaaatctg acgttaggct tgaaaaagta aattctaatt tggcgatttt
     2581 attgcccttt tttgctttta tttttgtgac tttaagatca ttgaagtatt tttttagttc
     2641 tcgtaatgca ggatttaata ctttttgatc aatgttaccc atttgatagt attctgggat
     2701 atccattaag ctcctaaact cttcaatacg aactacataa taaccagtag aacggtactg
     2761 cataagcagt cggaatagag tttttgcata cgtagaacgc aacgacgtga aagcagataa
     2821 ttcaaatcta ctaaattcag ctgttaaacc attaataata tattctaggt ccggattaac
     2881 actgacttca acaaactttt ttgttttatt gatttcaaaa ccagtaaaca aaacaaagta
     2941 gcgaatagaa tcctcatctt cagtacggta agtaagatta agcatatctt tatagagctt
     3001 ttctaaatca ccgacaaagg cttttgtagc agtcatctta taatcactta actcttttaa
     3061 ttcctcgaaa gaaaatctaa cttttttcaa accacgatct ttcatacgag cgcataatgc
     3121 aaaaaataaa tccatctgct tagcattgaa atttttcagc ggtactaaat tcaattcatt
     3181 ttgatatttg actgttaatt ccgt
//
LOCUS       DAXWOO010000218         3116 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.222, whole genome shotgun sequence.
ACCESSION   DAXWOO010000218 DAXWOO010000000
VERSION     DAXWOO010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.222"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(<1..2232)
                     /locus_tag="ACGE70_002706"
     CDS             complement(<1..2232)
                     /locus_tag="ACGE70_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFF8694298.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3116)
                     /locus_tag="ACGE70_002707"
                     /pseudo
     CDS             complement(2904..3116)
                     /locus_tag="ACGE70_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          860 a          703 c          514 g         1039 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg agtccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaata
//
LOCUS       DAXWOO010000219         3067 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.223, whole genome shotgun sequence.
ACCESSION   DAXWOO010000219 DAXWOO010000000
VERSION     DAXWOO010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.223"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            191..421
                     /locus_tag="ACGE70_002708"
     CDS             191..421
                     /locus_tag="ACGE70_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFF8694299.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            518..1819
                     /gene="murA"
                     /locus_tag="ACGE70_002709"
     CDS             518..1819
                     /gene="murA"
                     /locus_tag="ACGE70_002709"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFF8694300.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1832..2008
                     /locus_tag="ACGE70_002710"
     CDS             1832..2008
                     /locus_tag="ACGE70_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFF8694301.1"
                     /translation="MSNQRYILLTLIKILMVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2143..2439
                     /gene="yidD"
                     /locus_tag="ACGE70_002711"
     CDS             2143..2439
                     /gene="yidD"
                     /locus_tag="ACGE70_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFF8694302.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            2624..>3067
                     /locus_tag="ACGE70_002712"
     CDS             2624..>3067
                     /locus_tag="ACGE70_002712"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8694303.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIH"
BASE COUNT         1023 a          503 c          649 g          892 t
ORIGIN      
        1 ttatttggaa taagggactg cgacaagact tttgtctcag tccctttttt tgcatatttt
       61 tagcatttat ttcctaaaaa atgtaagggg attcctatat ttttaagtat acttgttttt
      121 ccttttatac attttatggt attatcattt tattagttat tataaaacaa tcgttagaaa
      181 ggacgtcatt atgcaagtat acggaattga tgcggtgctt cgaatcgtga gtcatttggc
      241 gtttatctac ttagccttct ggtctttacg ttcgttacgc attgaaactt ttttcagacc
      301 aatgcataat acgcaaatta gaatggcaat cgtacttttt tcaatttttt tagggtacac
      361 tgcaagtaat tttttcttag aagttatcgc tttatgccga aatcttttcg ttagcctgta
      421 aattttaaaa aaaggtacta ttttaaaagc aaggatgtta taatagaaaa gattttgagt
      481 aagtgtatgc tcattggaat tttttggagg gaacaacatg gaagagatca tcgtaagagg
      541 tggcaatcaa ttaaatggaa cagtaagaat tgaaggagct aaaaatgctg ttttgcctat
      601 tttagctgct agtttgttag cagaagaagg tattacgaca ttagacaacg ttccgattct
      661 ttctgatgtc ttcactatga atcaagtgat tcgtcactta aacgtagatg tagattttga
      721 tgaacaaaag aatcaagtaa caatcgacgc ttcaagacaa ttagaaatcg aagcgcctta
      781 tgagtatgtt agtcaaatgc gtgcttcaat cgttgtgatg ggtccgctac ttgccagaaa
      841 tggacatgca aaagtagcta tgcctggtgg atgtgcgatt ggtaaacgac caatcgattt
      901 gcatttaaaa ggatttcaag ctttaggagc aaaaattatt caaaaaaacg gctatatcga
      961 agcaattgcg gatgaattga tcggtaacac gatttattta gatttcccta gtgtaggtgc
     1021 tactcaaaat atcatgatgg ctgccgtaaa agcaaaagga acaaccatca ttgaaaacgt
     1081 agctcgcgaa ccagaaatcg ttgatttagc aaacattttg aataaaatgg gcgctcaagt
     1141 ctatggagct ggtactgaaa caatgcgtat tgaaggcgtg gatcatttac atgcagtcaa
     1201 ccactcaatc gtacaagatc gtatcgaagc aggtaccttt atggttgctg ctgcaatgac
     1261 acaaggcaat gtgctgattg cagatgctat ctctgaacat aaccgcccat tgatttctaa
     1321 attgatcgaa atgggtgcag aaatcattga agaagaaggc ggagtccgtg tcatcggacc
     1381 aaaacatatc ttacctacag atgtaaaaac gatgcctcat cctggtttcc ctacagatat
     1441 gcaagctcaa atgacagcta tccaattagt tgcagaaggt acaagtgtag tgactgaaac
     1501 agtgtttgaa aatcgtttcc aacatttaga agagatgcgc cgtatgaacg ctcatgtcaa
     1561 aatcgacgga aatgtagcaa tcatggatgg aaaccatgaa ttgcaaggtg cagaagtata
     1621 tgcaacagac ttgcgggcag cagcagcatt agttttagct ggcttgaaag caaatggaat
     1681 cacacgtgtg cgtaacttga attatctaga ccgtggatat tataatttcc atatcaaact
     1741 acagcaatta ggtgcagacg ttgaacgtgt agacatggat caaacttctg ctgaaaaaac
     1801 agcacaaacg attgcttaag gaagtggctg aatgagtaac caacgatata tcttattaac
     1861 tttgataaag attttaatgg tgatcttatt gcttatcctt ctttttgtgg ccggaacgat
     1921 gatcggatat ggtgtcatcg gtggcggcaa tccatttaaa gtatttcagc caagtttgtg
     1981 gattcatatt cgagactttt ttcattgata aataagaatc acagtggttc tagtcgatat
     2041 gaagtgagcc atgaagcaga acatgataaa agaaaacaga agggaacgtt gacgttagtc
     2101 gcgatccctt tttttagtaa aagaaaagaa ggaatctaat tgatgaagaa gatattgatt
     2161 tatttagtta gaggatatca gcgctttatt tcgcctgcat ttccacctag ctgccgttat
     2221 tacccaacct gttccaatta tatgataaaa gcaattcaaa cccatggcgc gctaaaaggg
     2281 acggtaatgg ggactgcacg gatcttacgc tgtcatcctt ttgtcaaagg cggtatcgat
     2341 tatgtaccat tgacattccg attaacgcga aataagaaag ataaaaaaga accagattat
     2401 aaaaaggttg tgaaaaatac gggtagcact gagcgataaa gaaacaattt ggaaaatagc
     2461 ttcttatatt ttgcaaaatt gtgagttatt gccgaagtgt tggattttga acaccgtcaa
     2521 aaaaaaggtt gtgaaaaata cggctagcac tgagcgataa agaaaaagag gaaatttatt
     2581 gtcaaagtaa caactccgca actacttata tgggggacaa aaaatgatcg aagaactaaa
     2641 caagaaagat ttaccatggt ccttgttatt agatgcagat cctgataaag aaaaagttca
     2701 agtatatgtt tccagaggga gtggctggat atggaaagaa aaagaaaaaa caataggtgt
     2761 actgatctat gtggcaaggg aaaaagaata cgaaatcgtc aatgtagcag taaccccaac
     2821 gtatcaagga aaggggattg gcggaaaatt acttgagaca gcttttcaga agctttcaca
     2881 actcgcttcc agtcaaactc agatcatcat acgaaccggc agtactagca gtgcggcgtt
     2941 acatctctat caaaaaatgg gctatgtgga gattggtcga gtcaaagact attttatcca
     3001 tcattactct gagcccattt ttgaaaatgg agatcaatta agggatcaag tgattttagc
     3061 aattcac
//
LOCUS       DAXWOO010000220         2998 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.225, whole genome shotgun sequence.
ACCESSION   DAXWOO010000220 DAXWOO010000000
VERSION     DAXWOO010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.225"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(42..254)
                     /locus_tag="ACGE70_002713"
     CDS             complement(42..254)
                     /locus_tag="ACGE70_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694304.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            441..908
                     /locus_tag="ACGE70_002714"
     CDS             441..908
                     /locus_tag="ACGE70_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694305.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSELNKYKLQKTSE"
     gene            905..1147
                     /locus_tag="ACGE70_002715"
     CDS             905..1147
                     /locus_tag="ACGE70_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HFF8694306.1"
                     /translation="MIASLFIYPEGGITVAKVKTPFEKAASKILERRGVSEIQWKKEV
                     LEKAQFDLLRGKDKDLEEYIYQREREKLVFDEIIKH"
     gene            1165..1467
                     /locus_tag="ACGE70_002716"
     CDS             1165..1467
                     /locus_tag="ACGE70_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694307.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            1539..>2998
                     /locus_tag="ACGE70_002717"
     CDS             1539..>2998
                     /locus_tag="ACGE70_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694308.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLIDLIR
                     LDVFKDTDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETDEVEVDPVEA
                     NEESTWTEGDFQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKVKEELNKEEQRVIEEEVQKVKERYEKKRDNAYSLCVE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQ"
BASE COUNT         1146 a          408 c          601 g          843 t
ORIGIN      
        1 aaaaataaga aaagccttat ataattttgg cttttcttat tttatttgtt agttgaagta
       61 ttaatatcaa cgatttcttc agccaattta tttaatttca tagcatttat gaatctcatg
      121 ttgtctatac tagagcgttt gttggcaagg tcacttactg tagttaaggg tacgccagat
      181 tctatggata tagaacgaac tgatttacca ctatcaaaca gcaaccattc gattttcttt
      241 ttatcagcgt acatttcaat gctccttctt gttttttctt tattatagct cttttttgta
      301 atgaatcaca atatcaagag aaaaattttc tatagaaagg tggattttta tgagtattcg
      361 ttctccgtgt atccaaatga ggtttgttgc tactttcgtt atttgatata ctaaaaataa
      421 acaacgaaag aaggaataaa atgaagaaaa agcaactatg gtttgtctct atgtttgcaa
      481 tattttttat tattttaagt ggatgttcaa gtaataagaa atcggaagct aaagaagata
      541 atagtttatt gcaaccagga actgtttgga aagaagaagt aggtggtctt attcataatt
      601 taaaaattat agatgatact acctgggaat attctgaaag tgtatggcat ccagatcctg
      661 tacaaataac agttaagagg caaaaagact atgaaggttt agaaagatac aaaatagtag
      721 attctgcagg cattagggaa tttataaaca aacctgattc attatttatt gttgttccct
      781 atgagaagga tggcgtaaaa aaaataattt ttcttggatc tagcaaggat gagaagcgaa
      841 cgaaagagaa attaattcat gatggatcag agttaaataa atacaaatta caaaagacta
      901 gtgagtgatt gctagtcttt ttatttatcc tgaaggagga atcacagtgg caaaagttaa
      961 aacaccattc gaaaaagccg cgtctaaaat cttagaacgc cgtggtgttt ctgaaataca
     1021 gtggaaaaaa gaagttcttg aaaaagcaca atttgaccta ttacgaggaa aagataagga
     1081 cttagaagaa tatatctatc aacgtgaacg tgaaaaactt gtattcgatg aaattattaa
     1141 acattaaaag gagcgaataa tcttatgttt gaatcaatta caatgaattt tttaactcaa
     1201 ccgaaagtag tagctgcagt tgatattttt agagcattac aaactgccgc aactagtttg
     1261 aaaaataatc tgatgatttt tgctgctgtt gcggcagtgg cttccattgc tcttggaggt
     1321 attttcttct tctttgggcg aagaggagct gaaaacggga agacacacgt aggttatgta
     1381 ttgttggggg taggagttat tttagctgcc gcttctattg tagtttcaat gttctcaatg
     1441 tttggacaaa ccccacaagg actataaaaa aaacaagtag gaggagtaaa aagtacgtgg
     1501 ctacttttta ctcctttttt tatgaaagga gaatattcat gttttttacg atttcaggaa
     1561 atgcaaaatt ggataaagag ttaataagaa aagcaacgaa gaaagtggga aagcgtactt
     1621 cctttttacc gaaaacaatt aaatattctg tcattgaaac ggaagaacag ctacatgtgt
     1681 taggacaagt gttagaacaa gagaatcaat cttctgttaa attgactctc acgttacatg
     1741 ataaagaagg aaaaatagaa aaggctggca cagaatacgt ttttggccca ctggattatc
     1801 aaatttcaga aggtttttat cctacattga tagatttaat ccgccttgat gtgtttaaag
     1861 atacggatga aactatgagt gtggaagagt cttatcgaaa cgaaaaactt ttacaagttt
     1921 tagaagaaga gttagagagc gaccaaagaa aagtgaatca agtgagtgaa gtcgaagtaa
     1981 atgaagaagt accatgggaa cgaccaatct ttccaaagaa ggagtcaaaa gaaaaagaga
     2041 cagtctatgt tgacgaacaa gctgatattc cgactcagaa atcgatattt gaggaagaga
     2101 acgatgtgat gggtcaattg gaggtagatc cctttgaaga aacaatggag gatgaagaga
     2161 cggacgaagt agaagttgat ccagttgaag ctaatgaaga gtcaacatgg actgaagggg
     2221 attttcagga atatattgaa ccacttatca gtgaagaagt agaaaaaaac gaaacagaga
     2281 tacaaaaggc aactgtattt gattatattt tggatcaata tccgccagag catgaatgga
     2341 tgaaagaaaa aaacaaatta tttttaaatg agctttattc gaaatatcaa ttaccccaaa
     2401 cctatcaagc atttttagag agtaaagaag aattaatctc acagggtaaa ggcacacttt
     2461 tagaacagtt agagtctgtc caatcaacaa attggcatga gcttgcacaa gaagatttat
     2521 tagccacctt tgaagaacgt caacaagaaa cagatcaaga aattaatgag tatgcgacgg
     2581 aacagtcgtc taattgggag aaagtaaaag aggaactaaa taaagaagaa cagcgagtga
     2641 ttgaagaaga agtacaaaaa gtcaaagagc ggtacgaaaa gaaacgcgac aatgcttatt
     2701 ccttgtgtgt agaaaagatc aacaatttta ctaagacgaa tcaagaagcg ttatcgaaag
     2761 aaaaagaaaa cttgttaaaa gaacgtatta aagaacgaaa agaagagttc taccgcggtt
     2821 tacgtacaga taagctaaag attagcaaag aattaaatga acaactgaat aagctatatc
     2881 aaacaacgac tgaagttctt atggaaaaac acgaacagat ccagcaagaa ttacaagtac
     2941 aaattccaaa atggaaaaaa gagtatgaaa ttcaacaaca agaattgaaa gaacaaga
//
LOCUS       DAXWOO010000221         2935 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.226, whole genome shotgun sequence.
ACCESSION   DAXWOO010000221 DAXWOO010000000
VERSION     DAXWOO010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.226"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            39..410
                     /locus_tag="ACGE70_002718"
     CDS             39..410
                     /locus_tag="ACGE70_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694309.1"
                     /translation="MRELTLSSKELEKKTALLNEIFSADCIIQSLKESSFSKLTDQEK
                     ISYMLSLDNLGTKYDESASSDKLLIELVSSLSSFGKMKRFNVILEIFAWLIPSFIWFF
                     YIFIVESELIAKVSLGLLIPI"
     gene            495..695
                     /locus_tag="ACGE70_002719"
     CDS             495..695
                     /locus_tag="ACGE70_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694310.1"
                     /translation="MLKRIAWDKDNKTDIELKEYKYNYFSRCIDRKLLFKKEGLEFSF
                     DEDEFEKLVMDWRKNLLDTVQF"
     gene            886..2055
                     /locus_tag="ACGE70_002720"
     CDS             886..2055
                     /locus_tag="ACGE70_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694311.1"
                     /translation="MTKKIEVYVDGSQEERDSFFDFFWKNSREYVKALNLVYKQLMFE
                     ELIKDAIKEMDQGYAEREESIQNRIEKLAYTDPIDQKKLDKAKAELSKLRNEKNKEAR
                     EILTQAIGLKSQTRTRDVAKKLDFDFGDFVDSANMKASKDFGNDFIGIVTGQRTPRIY
                     KNRNKYGLLPFRGRDIKIHKDDKFYIYLPKGFRFKVNLGSKPRKARDVASVLQHIMDG
                     DYKLNQSNLIFDGRKLFFNLTISFEKSYNEMEFKKGNELIVNFADLCSGECLINDKSV
                     MSFGDSQYIRNFKSKILAMKSKEQSRGVFSKGGHGRKRKLDQDRWEAIKKRESNFVKT
                     YNNQCSAKIVKTAIRNKAQIIRIIEPDKAYGEWAYYQLKEQLVNKGKRYGIEVRS"
     gene            2652..>2935
                     /locus_tag="ACGE70_002721"
     CDS             2652..>2935
                     /locus_tag="ACGE70_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694312.1"
                     /translation="MFDYSREPVNDYFLIDMKSFYASVECIERNLDPMTTPLIVMSRS
                     DNTGSGLTLASSPTAKKKYHITNVSRATDLPYPLPKELHIVPPRMNLYIK"
BASE COUNT         1156 a          341 c          520 g          918 t
ORIGIN      
        1 taaataggac acaaaatttg gaaagtaggt aagtcactat gagagaacta actttatctt
       61 ctaaggaatt agaaaagaaa actgctttat taaacgaaat atttagtgct gattgtataa
      121 tccaaagttt aaaagaaagt tcattttcaa aactaacaga tcaggaaaaa atttcttata
      181 tgctaagttt agataatcta ggaactaaat atgatgaatc agcaagtagc gataagcttt
      241 tgattgaatt agtatcaagt cttagtagtt ttggaaaaat gaaaagattt aatgtgatat
      301 tagaaatttt tgcttggtta atcccttcat ttatttggtt tttttatatt ttcatagttg
      361 aatctgagtt aatagccaag gtctctcttg gactgttaat tccgatataa ataggttcag
      421 caatttactt taataacttg tatacaccta ttcaaataaa aaagcagtta ttacatgaag
      481 gattaagtgg taatatgtta aaaagaattg catgggataa agataataaa acagatattg
      541 agcttaaaga atataaatac aattatttct cgagatgtat agatagaaag ttactgttta
      601 aaaaagaagg attagaattt agttttgatg aagatgaatt tgaaaaatta gtgatggact
      661 ggcgtaagaa tttactggat acggtacaat tctaaaagca attgaggtcg aaattccgat
      721 acttgaatca tgatctatat tatcgtgtta tgagtgaaat ttctaataaa gataagatat
      781 ttcatttgga cagtcatgtg aatatgagct taacaattaa acgtatttat gatcttttat
      841 atgtttataa acattaatga ggtgctaaaa ttgacaacaa taactatgac aaaaaaaata
      901 gaagtatatg ttgatggatc acaagaagaa agggattcct tttttgattt tttttggaaa
      961 aatagtagag aatatgtaaa agcgttgaat ctggtatata aacagctaat gtttgaagag
     1021 ttgataaaag atgcaattaa agaaatggat caaggttatg ctgagagaga agaaagtatt
     1081 caaaatcgga ttgaaaagtt agcatatact gatccaatag atcaaaaaaa acttgataaa
     1141 gcaaaagctg aattatctaa attgagaaat gaaaaaaata aagaagcgag agaaatatta
     1201 acgcaagcga tagggttgaa gagtcaaacg agaacacgag atgtagcaaa aaaattagac
     1261 tttgactttg gagattttgt tgattccgca aatatgaaag ctagtaaaga ttttggcaat
     1321 gattttatcg gaattgtgac cggacaaaga acgcctagaa tatataaaaa tagaaacaaa
     1381 tatggattat taccttttag aggtcgagat attaaaatcc ataaagatga taagttttat
     1441 atttatttac ctaaaggctt taggtttaaa gtgaatttag gtagcaagcc aagaaaagca
     1501 agagatgttg cttcagtttt acaacacatt atggatggcg attacaaatt aaatcaaagt
     1561 aatcttattt ttgatggaag aaaattattt tttaatctaa caatttcttt tgaaaaaagc
     1621 tataatgaaa tggaattcaa gaaaggtaat gaattgatcg taaactttgc agatttgtgt
     1681 agcggtgaat gtttaattaa tgataaatct gtaatgtctt ttggtgatag ccaatatata
     1741 agaaacttca aaagtaaaat tttagcaatg aaatcaaaag aacaaagccg aggagtcttt
     1801 tctaagggcg gtcatggtcg aaaaagaaaa ttagatcaag atcgatggga agcaataaag
     1861 aaaagagaaa gtaattttgt taaaacttat aataatcaat gttctgcaaa aatcgttaaa
     1921 actgctattc gcaataaagc tcaaattatt cgtatcattg aaccagataa ggcttatggc
     1981 gaatgggctt actatcaatt gaaagaacaa ctagtaaaca aaggaaaaag atatgggata
     2041 gaagtcagaa gttagtaaat taaacaatcg ttgatttggc aacgaaatag tgagggaaaa
     2101 tccactcact gaagggtaaa ctccgtcaag ttgtgtgacc agacggaagg acgaatgttg
     2161 ttgaggtttt agaaccatat aaaacagcat gtttctcaaa tttttaggat aagggaaatt
     2221 gctagcagtt tgttttagaa ccatataaaa cagcatgttt ctcaaatgag tactttaaat
     2281 taattgatgg cgttcctgct ttagaaccat ataaaacaac atggatttaa aacaagaagt
     2341 aagtcagttc tccttttact ttagtaagga aaaataaagt attctcaata aaacttaaaa
     2401 taagcaaacc tttgttttaa aatgattgtc aagttaaaaa aatctgggat catataaaat
     2461 agtatagttc tcaaatatga ttagaaaccc tattaaagag caatattaag ccaacttact
     2521 taaatcgttt aattaagaac aataatctct tttagtcaga cttttagtct gacttttttg
     2581 tatttccaaa atttattctt tacaaaaacg aacatacgtt tgtataatta cttgtgaatg
     2641 ggggaataaa aatgtttgat tacagtagag aaccggtgaa tgactatttt ttaatcgaca
     2701 tgaagtcttt ctatgctagc gtggaatgta ttgaacgaaa tttagaccca atgactacac
     2761 cgttaattgt catgagtaga tcggacaaca ctggttcagg tctgactcta gcttcttcac
     2821 ctactgcaaa gaaaaaatat catattacta atgttagtag agctactgat ttaccttatc
     2881 cattaccaaa agaacttcat attgttccac cacgaatgaa cttatatata aaaaa
//
LOCUS       DAXWOO010000222         2897 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.227, whole genome shotgun sequence.
ACCESSION   DAXWOO010000222 DAXWOO010000000
VERSION     DAXWOO010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.227"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..363
                     /locus_tag="ACGE70_002722"
     CDS             <1..363
                     /locus_tag="ACGE70_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694313.1"
                     /translation="EIISTHEDISNYSEAVRHLCMMYDPKIEEKINPIQIKLNAMGKE
                     ISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVEGKIKSNQTKKYSSKLKKPVN
                     ESVHTRVDPFDPTKILKR"
     gene            382..1974
                     /gene="mobP2"
                     /locus_tag="ACGE70_002723"
     CDS             382..1974
                     /gene="mobP2"
                     /locus_tag="ACGE70_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobP2 family relaxase"
                     /protein_id="HFF8694314.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            1986..2321
                     /locus_tag="ACGE70_002724"
     CDS             1986..2321
                     /locus_tag="ACGE70_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694315.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            2468..2662
                     /locus_tag="ACGE70_002725"
     CDS             2468..2662
                     /locus_tag="ACGE70_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694316.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            2694..>2897
                     /locus_tag="ACGE70_002726"
     CDS             2694..>2897
                     /locus_tag="ACGE70_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694317.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQS"
BASE COUNT         1185 a          432 c          568 g          712 t
ORIGIN      
        1 gaaataattt ctactcatga agatatatct aattatagtg aagcagtcag acatttgtgt
       61 atgatgtatg atccaaaaat cgaagaaaaa ataaatccaa ttcaaattaa attgaatgca
      121 atgggaaaag aaatttctat tttaaccgaa atggtttcag agtttgcgga tcttcatttg
      181 aaagatacag gaatactaat tggtacaaat tcaactgttt atcaagaagc agaagaacgt
      241 gtggaaggaa aaattaaatc caatcaaacc aaaaaatatt cttctaagtt aaagaaacca
      301 gtgaatgaat ctgttcatac cagagtagat ccatttgatc caacgaagat tctaaaaaga
      361 taaaagtaga aggagaacgt gatgaataaa tcgcctgcag ttattcttac ttgtcaattt
      421 accttgccca ataaaaaatc tttttcaaca tacattgatt atatgacaag agaaaaagca
      481 ctggaagaaa tggaacgacg tacgcccgaa gaagaacagg aactgcaacg aatcaagaat
      541 gctttagagg atttttatat tcctgaagga gaaacttttt cggaaagtaa gacggataaa
      601 gaattcagtg aaacgtcgaa tgaagctcaa tcgttgatgg aacatggaat caattttgac
      661 aagttagagg aacaagattt taccaaatat ctttcttata tgacccgtca ctatgcgtta
      721 gaacaaaaga aagaactgac gcaaagggaa caaaaagaat accaacggtt gaaccaagca
      781 atgaaaaaat atcaaacaga gccacataaa aaagcttcag gaaatcttcc tggagttttt
      841 tctatggctt cagacgaagt gaaaggaagt gatctgaagg agatcaaaca aatcttccaa
      901 agaggacaac aaaaaggctc aatcatttat caagatgtta tctctcacga caattcgtat
      961 cttgaaaaaa tgggcttgta cgatcccaaa acagatacct tagatgaaga aggtttgaaa
     1021 gcagctggaa gaaaaatgat ggagacatta ttcgagaaag aacaattgaa tgaaacaggt
     1081 tactggatgg caacgattca ccgtaacact aagcatatcc atattcattt tgcagttgtt
     1141 gaaaaagaaa atacgcgtaa accgtatact gagattgaaa atggtgtgag ttatatcgta
     1201 ccgaagggga agagaagaca agcaacatta gacgcaatga aaaccaatta tgtacatgag
     1261 ttagaacgtt tctcttatga aaaggatcga atgatcggta tggagaaacg aaatttattg
     1321 acacgaaaaa gtgatttgcg taacacctta acaaaagtag taaaagaacc agcacgttac
     1381 gatcgacacg ccatgaactt attaaatgaa gtatataaaa ccctacctga aaaacgttcg
     1441 gaatggaatt atggagatga aaatcgaacc aagctttcac catttacaag gggaaaactt
     1501 gatgagttaa ccaaatatgt tttaacaaaa gaacctggat atcaagaata tatacagatt
     1561 tcaaaagcat tgaaagaaga agccaaaagc ctttatgggg aaagcaaaag agaaacaaag
     1621 aatgcagaaa aaaacgccct ctttgattta aaaaagcgca caggaaatgc gattttaaca
     1681 gaactgaaga gacaggatag tcaaataaaa ccattaatag aaagtgtcaa tcagatgcaa
     1741 tctatttaca atgaacgatc ctttcgaaca ctcaaacagt tgccaacaaa tgaatacaag
     1801 tatagttatc aagaattcgt aaagcgtagg aaacaattcc aacaaagagt tgtcacgcag
     1861 cgaagcttgc gtcaattaaa tcgaacaatt aatgcacatg aagaaaaata ccacgcaagt
     1921 aaagcatatg aagagcaaca gcgaagaatc caaatggaac aagaaagaca ataggaggaa
     1981 gagaaatgaa acaatattta gatcaatgga aagtcataga aggatcacta aaagaagaac
     2041 gaatcgaaca acttcctgat tgtttagaaa aagagcattt gtttcagata agcgaaatgt
     2101 tgagaaacga acagtttgat ccaaatcaat ttttagttgt tgagtaccca gctactggtg
     2161 tgtattgttg taatcatgtg aagggtgaaa aatattttat tattcaagaa tacgaaggta
     2221 agttagcgcc ttattatact acttgggaaa taaatgaaga gggaatcaat aatttcccat
     2281 gtgaaagcat tgaagagtct atttctctca cggaatgtta aataaataga gacgtaaaag
     2341 agtcctgaac gtgattgtcc aggactcttt ttcaatgaat tgttttccgc caattcactg
     2401 agtggtgcga ttcaatttta ttatatcaaa agaaagtgag attccaaagt gaagggagga
     2461 agaagaaatg actcagacaa cgattggtgt tgtggttgaa aaaagcaggg ataatctcgt
     2521 ttttgtgaca gaaattcaaa caggtagagc ttttgtagtc acagacaaag ctgcaaaagc
     2581 gtatcaaaat ggcaatattt taacgttaaa tctgactacg aagatgttcg ttgatgcagt
     2641 agaaaattat ccatttgttt agacaaacga tattttacaa ggaggaaacg gacatggcag
     2701 aagaaaaaga accactagat aatagtcggt tgggaaagtc caaaagaaaa ttagtacggt
     2761 tgcaaaacga attaaataat caatttcaaa agctatttga tcaccaaaaa ttgacaaatg
     2821 ggcaaccaat gaatgacaaa cgaaatggtc gttcatggtt taaccagcaa gaaaaaatag
     2881 aaaataaaat acaaagt
//
LOCUS       DAXWOO010000223         2878 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.228, whole genome shotgun sequence.
ACCESSION   DAXWOO010000223 DAXWOO010000000
VERSION     DAXWOO010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.228"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(<1..442)
                     /locus_tag="ACGE70_002727"
     CDS             complement(<1..442)
                     /locus_tag="ACGE70_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFF8694318.1"
                     /translation="MTYVFLPKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISV
                     FGDVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILAVATVGLYGIGDCI
                     FTGLFSINTNESSWNLSTWIHNTGSGLGYAGFLLFPLLLVLLYRQ"
     gene            complement(500..1552)
                     /locus_tag="ACGE70_002728"
     CDS             complement(500..1552)
                     /locus_tag="ACGE70_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFF8694319.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(1565..2503)
                     /locus_tag="ACGE70_002729"
     CDS             complement(1565..2503)
                     /locus_tag="ACGE70_002729"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFF8694320.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
BASE COUNT          976 a          613 c          449 g          840 t
ORIGIN      
        1 tttgacgata aagcagaaca agtaacaatg ggaaaagtag aaagcctgca tagcctagcc
       61 cagaacctgt attatggatc caagtcgata gattccaaga gctttcattc gtattgatac
      121 tgaataaccc agtaaaaata caatcaccta tcccatataa accaactgtt gcaacagcaa
      181 gtattgcaag tgtttttgat gttccaacaa ataggtgata caacgcaggc aacgaaagaa
      241 caaaaaaaag tccagataca actgaccaga ccaagaaagc acgtctgact ggactatcta
      301 cgtcaccaaa cacactaatt acggtagtca tttgattcag tttgggataa aaaaggccaa
      361 ggatatacgg tgtcgataaa tcactaatca cacctaataa taaaaaataa aaaccatatt
      421 ttggcaaaaa aacgtaagtc agatgttgtt gacatctcac ttacgcatcc attgattgaa
      481 aatttcttag taaaatctac tagcctaaaa atttgaagac ttcttctcga tcatacagtt
      541 taaatacttt ctcgcataaa aattgagtaa ttagtgccgc aaaaacaggg atgactaacc
      601 aacaaagaac aacaagaaag ccatttagcc ctgcatctaa agaagccaac ggaccaacaa
      661 gtccgaccac accgaaacca gcagaagcag gcgtacctga aatagaaaat agagcgacag
      721 gaatagctgt aatcactgca ctaactaaac aaggaataag aatcacaggt tttctaaata
      781 gattaggcat catcatcttc atagcgccta aagcaatcgc aattgtcaca cctgacttat
      841 taaccttcca agagttcact actaaaacaa aagtggttgc agctacgccc atcgcagccg
      901 caccagcaga aacaccatct agttggattg ccattccgat tgctactgtt gaaataggag
      961 agataatcaa aaaggcaaag gaacaaccaa tcaagataga cattaacagc ggttgtaagg
     1021 tagtgaagtt attaatcaat gatccaattg cagaagtaat tgctgttaca aaagggaata
     1081 aataaaaacc aagtaaacct gcacctacac ctacaacaat cggtgttaac acaatcgaca
     1141 ctgacccaaa actatttcca atcagtaaaa tcagcccgac agcaatcgat gctgtcagca
     1201 tcgtattaat aatatcgcct gtaccagcac caacgtaaac acccgtttca cccaaatcag
     1261 gtgtgaattt gactacacca gaaccaacaa aagaagctcc ggctacaacc atcattctca
     1321 tcggatcaaa tccaaattgt aaggcaatca aaccaccaat gatcaaaggt gttgctaatt
     1381 gaaaaataac tgctaactgt gcgatcgtta ccgcaaaggg atacgcacta aaatatttta
     1441 aaatagcacc taatactgca ttaggaatca atccaataat gattcctgtt gctgtaccag
     1501 cgaggacttt attcagaaaa attttcggtg atattttttg tgaagtttcc attcaatctc
     1561 tcctttattt tacgttcaaa ctatcttctt ttgcatggac aagctcagtc aagaaatcac
     1621 aataaggcgt cgcaacacca tatttcttcc cttttctaga aattgcgcca ttgatataat
     1681 cgatttctgt taatcgatgg ttcttaatca agtcttgata cattgaagga taatgtaacc
     1741 caattgtttc cgggtcaaaa caagattcac aatgtgcaat gacttctggg acatctagtt
     1801 caatcttctc tactgctgct actttggcaa actcattgac aatcgtcgcc accattttat
     1861 gagcagtcga tgtttttcct agctcagcca tattaacgtc taagattgtg catagtccgt
     1921 tcattgttcc gttcacacat gctttgcgat aaatagaata gtgaatattg tcagaaaaat
     1981 gagcatttaa tcctgattca gacaatttgt ctgctaattt ttttgcagct gcttcttttc
     2041 catcacctaa attttgtaat tctactgaac cacttccaaa taatttgacc tgaccaggac
     2101 cttcaagacc tgccgtccac atggtatttc caatatagat attttccatt ggtacaaatt
     2161 tctcaataat atcttcatgc ccgataccat ttaatagaca taagacctct gtatcttttt
     2221 tgattaatga ttggatatcc tgcagcattt tttccagctg catcgctttg gtaaatagaa
     2281 taatcagatc aacttgatct tctttttcta cttcggattg aagaacgatt ggtagttttg
     2341 cttctacctc tttaccatta aaatttgctt gtaatccatg ttctttgatt tgttggacat
     2401 gttctgccca gccatcgatt aataatactt cgttcccact ttggtgaagc attaacccaa
     2461 agcgacttcc cattgcgcca gcgcctgcaa ttgctatttt cattgattct tcctcctttt
     2521 tctttctatt atacttattt gagaatcatt tcacaacctt gttttgttat tcttctctta
     2581 tttatcatgt ttatatccac cattcaagtt attttatcag caaatcaata aaaactggaa
     2641 tagaatccca atttcatcgt aagagaaact agactagcaa taagaaaata gaaacattca
     2701 tagaaaaaac ctcactcctc tgattataaa agaggggaca ttaacctcgt gtatactaaa
     2761 accttccatt cattcgatgg atgattttct ttttgtgaaa aaatattcat tgtagtacga
     2821 tgttgcgcaa cacagacgga tggtattgct tattatttat taaaatcgaa caatacac
//
LOCUS       DAXWOO010000224         2852 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.229, whole genome shotgun sequence.
ACCESSION   DAXWOO010000224 DAXWOO010000000
VERSION     DAXWOO010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.229"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..134
                     /locus_tag="ACGE70_002730"
     CDS             <1..134
                     /locus_tag="ACGE70_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFF8694321.1"
                     /translation="VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(220..1314)
                     /locus_tag="ACGE70_002731"
     CDS             complement(220..1314)
                     /locus_tag="ACGE70_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFF8694322.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1503..2264
                     /locus_tag="ACGE70_002732"
     CDS             1503..2264
                     /locus_tag="ACGE70_002732"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFF8694323.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2379..2819)
                     /gene="fabZ"
                     /locus_tag="ACGE70_002733"
     CDS             complement(2379..2819)
                     /gene="fabZ"
                     /locus_tag="ACGE70_002733"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFF8694324.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
BASE COUNT          913 a          567 c          481 g          891 t
ORIGIN      
        1 aagtgaatca gacatttatt tccagtgtca gcaatcaacg taatcatatt ccaagaaaat
       61 cattgaatta cagaacacca atcgaaatat ttttgagcta tgtacaagaa gcattttatt
      121 ctagcttaat ttgacaaatc ataacctata aaaaaacaaa ataaaaaagc agaaataact
      181 ggctatttct agcggattat ttctgcaata actaattatc tattttttat tttcaattaa
      241 tttaaagctt gaccatggtt tcaaaaagtc aaggaggaat aattcgtcat cggagattct
      301 tcctacaaca ttgacacgtc cgtcgttttt catatccttc aaggcgattt gtgtttctcc
      361 tttgtattga ccgtaccctt cattgtcaat cagtacatcc ccatgatgca tatctcgcgt
      421 attatgaggc gggaaatctt tatctttgta gtatacacga gtcatcgttg aacgaaggat
      481 atattctgag cgatcccctc gataactatg aaggttatca aacaagcaga ttcgttcgtt
      541 ttctgtaata ccttcttccg tatctacttt caatgttgga taatccgcgt taaatgcttc
      601 tgccatttct ttcaattctt cttcacttgc gtacgcattt ccaatagaaa tatcatcaat
      661 caatcctgta aggattaaat gcttcacttg tgtcgcaatt tcaagatctc ggtggtcttc
      721 cagcgtgcac aagccatctt gcgtaggcca agggccaaac tcagcagatt gcgaattgac
      781 gaatgccatc gtattcaaat tatattttct gaatttttct gaacaaaaaa caaagtgttc
      841 gtatcctaaa ccagagtatc gatgaggata aaaattatga gaacctaata aattatccgt
      901 gtttggcgaa taactcatga tgttgtcaac gtaattcgtg cccgaactca tattgatctc
      961 gattttgata ccaaaaggat tgcgcgtcat ttttgcttct tctgctcctg tgaatccgat
     1021 atctaagcgg accccgtagg cacccatctt atggaaaaaa gataagtcat cgtaagaaat
     1081 ctccaactgt tcaaataacg ctggattgat atcaaccatt acttccatgc ctaggctatt
     1141 cgcgtaatca actacttctt taaattctga aagaactttt tctttatcat cattgatctg
     1201 cagcaaactg gtaaaaaccc gtttataccc atacttatga gctaaatcca agtacgcttt
     1261 gtccttttca aatgttgacc gttctggata aatcgagatt cctaattttc ccatattacc
     1321 atttcctcct gcatcttttc gtttttgtat ccacttacat tatagcaaac acaaagtaaa
     1381 tggtctacac cttttctttt tcttttaaaa taaatgataa attccaataa tttaagtagg
     1441 tattttccct ctattttgtt atcattaaag ataacaaaat agtaaattgg aaaggaacag
     1501 ttatgaagaa aaataaagtt attttaagta ttagtttagc agcagcattg ctgtttgctg
     1561 ctggttgttc tgcaaacaac acgacttctt ctagttcgtc tacagaaagt tcaaaaagta
     1621 catctactgc ggaaagcacg actgccagta gcgtcgattt gaacgcattg gaattaccac
     1681 aattgacgaa agatgtcgcc gaaaatgaag atctggtcga aatggtgacg aataaaggca
     1741 caatcaaaat caaattattt cccgaacacg caccaaaagc ggttgaaaac tttatgacac
     1801 atgcaaaaga cggttactac aacggactga ctttccatcg cgtcatcaaa gatttcatga
     1861 tccaaggtgg cgatcctaaa ggcgatggca ctggagggga aagcatttgg ggagaaggat
     1921 ttgaaacaga gatcaacaac cacctttata atataagagg tgcgttatct atggctcgct
     1981 cacaagatcc taacagtaac ggcagccagt tctttatcgt tcaaaataca gatgatatgc
     2041 atgatggtct tttaaaagac gactatcctc aagcaatcat cgacgcttat aaaaacggcg
     2101 gttatccttc actagatggc cattatactg tcttcggcca agtgattgaa ggaatggatg
     2161 tcgtggatgc tattgcaaat atcgaaacag atagttcaga caaaccaaaa gaagaagtaa
     2221 aaatcgataa aatcaatata ctgcaagaag caaaacaaaa ataaaaaaat aacgtctgaa
     2281 tcctgcatag atgacaacgt atgctaggat tcaggcgtct tctttttaca taaaaagcaa
     2341 gtaagcttcc ttttgttttg ctatatcagg ttttatagtc aagcattttg gttgacgatg
     2401 aacgtgaact cgcactcaca gactttttta tcatctacat atgctgcaga taacgctgta
     2461 ccgactgatc ccttcatttt ggtgatttca aaatgacatt tcagcacatc tcctggaaca
     2521 actttttgac ggaatttggc cttattgatc ccaccgatat aagctctttt tcctagaaac
     2581 tcttctgatt ttaggataag tatcgaacct gcttgtgcca gtgtttctag aatcaacgca
     2641 cccggcatca ctggattgtc aggaaagtga ccttggaaaa aatcttcatt gattgttaca
     2701 ttttttgtcg caacgatctt ttttcctggt tctaaagtat ctacataatc aatataacaa
     2761 ataggataac gattaggaat caattccatt acttcagtag cagataatac gtaagacata
     2821 attaaactcc ttctttcagg caattgccat tt
//
LOCUS       DAXWOO010000225         2713 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.230, whole genome shotgun sequence.
ACCESSION   DAXWOO010000225 DAXWOO010000000
VERSION     DAXWOO010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.230"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            167..676
                     /locus_tag="ACGE70_002734"
     CDS             167..676
                     /locus_tag="ACGE70_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694325.1"
                     /translation="MKKVKREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            673..1977
                     /locus_tag="ACGE70_002735"
     CDS             673..1977
                     /locus_tag="ACGE70_002735"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFF8694326.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            2073..>2713
                     /locus_tag="ACGE70_002736"
     CDS             2073..>2713
                     /locus_tag="ACGE70_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFF8694327.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSDAENGASETETTSNVPIDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLANLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKA"
BASE COUNT          936 a          433 c          552 g          792 t
ORIGIN      
        1 ataaaaaata aaaagaacaa ctgataaaaa gaacaaaata tttttcattt gctgatgaga
       61 aatgttttgt tctttttctt aggaaaaatt taggaacact ttatgtgaaa aaaatgaaat
      121 gtatggtaaa ctcctacgtt gtaacaaaaa aatgtaaggt gaggatatga aaaaagtaaa
      181 aagagaagaa gttgcatcaa ttttggtgaa agataagtat ttttcaaaag gtaattactg
      241 gttaaagatt tggcaaacgt tagtggctct tttaggatgg atgatcgttg ttcttccttt
      301 tatatgggtc tttttcccat taacgagacc agaaagagcc aaggattttt ccttgtacgc
      361 ttttttatca gaaaagaaaa tgctgtattt cttaatcggc ttttttgtat tgattttttt
      421 cagtttcctg attacctcat ttgtattgac taggtggaac aatcgaaatc tatacaaaaa
      481 agtaaataaa tcttttgaat atgaagatga aaaattgaaa aaaagacaag aattacttga
      541 agaagtgtat acggaacgtt ttggaacaaa agaagagaga gaaacggtcc gtttttattc
      601 tgtctccgaa gagaaaaatc tagatacgac attcattgca gatttgtata aagaaaatgg
      661 ggtggaactg aaatgatctc aatcacccat acaggtaatt tattcttaga tacttgtttg
      721 tctattatgt atttttttct cgtttcttat ccgattttag gcggatttgt atggtttatt
      781 ggtgtctggt gttatgtgtt tttatacaaa cataagcaaa aagaatgggt agatgttccc
      841 ttgagtgtcg aaccgttcat cacgatcatg gtgccagccc ataatgaaga aattgtgatc
      901 gaatatacaa tcgagtattt gatgacgaaa ttgaattatc acaactatga ggtattagtg
      961 acagacgatg gttcaacgga ccaaacacca gaaatattag ctagactgat gaaaaaatac
     1021 gctaatcttc gtgtggttcg gatcgaaaaa aataaaggaa aagcccacgc gtttaatatc
     1081 ggacttgcct ttgcaaaagg gaaattgatt ttaagcaatg atgcagacac ggtaccggag
     1141 ccagatgcac tgattaggta tgtgaattat ttcattcgcc ctggtgcaag acatattgct
     1201 gcggtcactg caaatatgga tgtccaaaat cgtaccaaat tgattgcaaa atctcaaacg
     1261 gttgaattct caagtatcgt gggcatcatt aaacgaacac agtcagctgt tttcggtggt
     1321 ctttatgcgt acagtggagc aaatacgatg tatcgaaaag aagccttgat cgatgtgggc
     1381 ggattcagac aagatcgagc gacagaagac atcagtattg cttgggatca tcagttaaat
     1441 gactggttat ctgtttttgc tcctgagatt atctttttca tggaagtacc tgtgacgttg
     1501 aagatgctat atcgacacag aaaacgttgg gcaaaaggtg gaacagaagt ctggctgacg
     1561 aatttcaaaa aagtgatgct ccatcctttt aaacatattg gaagaacgat catcttcatt
     1621 gatcaaacat tgagtattgt ctggtcaata ttcttttgta tttccgtagt tctttttgct
     1681 ggattaatcg ggcattatgt atatcaagga aactacgaac agatttatat tacttttaca
     1741 ttcagttttg tgttcatctg ctttgaaatg gtcgcaggat ttttccagtt gctagcttcg
     1801 ctgattgtag atgatcgacg aagaaaattg aaataccttc ttttcgcacc actatatatg
     1861 cttttgttct ggatagtgaa cgcgataacg atcgtgacga ctttcattcc agcagtcaaa
     1921 acgattttag gttatggaag cgggacatgg aaaagcccgg aacgtacaaa aaaataaaag
     1981 atgcaccgtt ttttagacaa acagcctgtt gtgtttatgg ttcttccaaa catttttgct
     2041 tataataaga aattagttaa aaagtagtaa ggatgggaaa agtgatgaaa aaaagaatga
     2101 agccgttaat ctggccaatt gctgtttcca gtattttctt attgggacag acagcttttg
     2161 cggaaacttc tgaacctgct caaacagaac cttcaacaac tataagctct tctgatgctg
     2221 aaaacggtgc ctcggaaaca gaaacgactt ccaatgtacc gatagataat tcaggagaaa
     2281 cgatttcatc ttcagaagat acatcaataa atagttctac ctcttctgac caaatcgatc
     2341 atttgcaaga cttggaaaat aaaagaaaag aactcgaaga agcattagaa gctgcaaaaa
     2401 acaaattgaa agaagaccaa gcagcaatcg atcaagattt agctaatctt aaaaataaag
     2461 tgggaaatga ccgtgcacaa cttgagaaaa agcttgccga agcgaaagca aaagcggaag
     2521 ctgtccaaac agatcttgac gggaagatcc aacaagcaaa agaattcaat cagcaattaa
     2581 atcaggttgc tcaaacagct tctggaaagc taagtcaaac tcaagcggat atcacagcgg
     2641 ccagtcaagc aatccacgga aagatcagcg aagcacaaac agttgctaat gattttgaag
     2701 caaaaaaagc tga
//
LOCUS       DAXWOO010000226         2621 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.231, whole genome shotgun sequence.
ACCESSION   DAXWOO010000226 DAXWOO010000000
VERSION     DAXWOO010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.231"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            109..615
                     /locus_tag="ACGE70_002737"
     CDS             109..615
                     /locus_tag="ACGE70_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFF8694328.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            632..1531
                     /locus_tag="ACGE70_002738"
     CDS             632..1531
                     /locus_tag="ACGE70_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFF8694329.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1618..2502
                     /locus_tag="ACGE70_002739"
     CDS             1618..2502
                     /locus_tag="ACGE70_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFF8694330.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
BASE COUNT          956 a          486 c          514 g          665 t
ORIGIN      
        1 cttctttttt gtatataaaa atcccaagta aatacataaa aaataaaaaa attgaaactt
       61 ttagcgcacc ttgtacgtca tattaagtga ggcagaaagg agtggagaat gatttttact
      121 ggatataaga aaaaacggca gatcgaaaaa taccgtcacc tgctgaatgg actcatcgaa
      181 actgattatc aaaaaatgtt ccgtatcacg atgaattacg tacatgataa agaagaggcc
      241 ttagatgtga tgcaagatag ttttcataaa gccctgaact attttgaaaa agaaaaagag
      301 atcgagcatt ttagtgcatg gttttatcgg atattgatcc gcacggcatt agatagttgg
      361 cgcagacaaa aaaggacgcc tgtggactta tttgaaattc aagatatccc ccaacagtta
      421 cctttagaaa cttccgttgc agagctccat atgattttag ataaaataga cagtccggaa
      481 aaagagattc tgatcctcta cttttttgaa ggatttcgtt tgaaagaaat tgcgactatc
      541 ttggatatga atgaaaacac agttaaaacg aaaatgtacc gctcattgaa tgctttacgt
      601 caaatcctag aatgaaggag agaagagtgc catgtattcc caaaaagaaa atttaaaaaa
      661 actaaagaaa acttataaag aacaagcaat tcccaaaggg cttaaagagg agatccgcca
      721 gcgttttatt atcgaagagc aatcatttat aaaagcaaag cggagaaaga gacgtctcca
      781 agggttcgga gtatctttgg ctatcgtaat gattacaaca agtagccttc tttttaatag
      841 tcaagttcga agctttgcag aagatttacc catcttagga tcagtcattg aattgatctt
      901 aggcgaacgc tttactgatc gatctgaaaa aatcgatatt caggtaccga agatcaatac
      961 acaaaatgag aaagaaaata aaacaattca tggattaaat caaaaatact ttcgcgaagg
     1021 acaagcagat tttgaaaaag ctgaaaaaga atatggaaat tttgaaacgg atcattacca
     1081 agttttggga gattatcaga aaatcgtaga cgataaccgg tttctcgtga ttgaaaggca
     1141 gatcacccaa acagcagcag atagccatgt tgaaaaaaga tacgacacaa tcgacaagaa
     1201 aaacagtgtc cagctctcac ttccactttt attcaaagat gacacctatc tatctgtttt
     1261 gacaaaagaa gtcaaacggc agatggccga acaagtcaag gaagatccat ctaagtacta
     1321 ttggactgag caagatattc aagaagggac gatagaaaaa ccaacacttg ttacgcctac
     1381 acgaagtttt tatttgaaca aagagcatca attagttttg actttttctc aatacgaaat
     1441 tgctcctggt tatatgggta caccagaatt tgtcattcct aagtccgtga caaaaacgat
     1501 tttggcatcg gaagattatt tggatcatta gacgagaagt agttgcgact agcagtttca
     1561 attttcaagt aagtacaagt aaataataag aagagaaaaa gagaaagaag agatatagtg
     1621 aaaagaaatt atttagttat cagtattatt gctttatccg tgcttttgtt tttcgaaatt
     1681 acttcggtgg cctccttgat caccgaagta aaaggacaaa cacactcttc ggagcattcg
     1741 tcacaatctt tcaaacaatc agaacaatca accattaaaa gccaatcaac tgaaacagct
     1801 aacgtcaaag aagccattca agaatcagcg gaaaaggtac aaagtcagtc atcacaatca
     1861 acacaagaca gtacaaaaac gagccgagat ccggctgtca aaagcgtcgc aatcagcttt
     1921 gatgatggac caggagcgac cacaacaccg cagcttctgc gaatcttaaa agaaaaaaac
     1981 gtgcatgcca cttttttcgt cttaggagaa aatacggcgc agcatcctga aatcgtgaag
     2041 cagacagctg aagcagggca cgagattggc aatcacacat acgatcatca gaatctggcc
     2101 actctttctg cccagtcgat gacggaggaa gtgacgaaag cagacactga gatcaaaaaa
     2161 gtcactggca aaacccccac ttttgttcgt ccgccctatg gttctgtcac gaatgtagga
     2221 gcaacgatca tccagcgacc gatcatcgaa tggtctgttg attcagaaga ttggaagaca
     2281 agaaatcctg atttgatttt gcagaagatc caagcaacag tatacgatgg ggcaattatt
     2341 ttattccatg atatctatcc tgaaacgatt cgtgccgttc ctcaagtgat cgattatctg
     2401 aaagagcaag ggtatcgcat cacgacagtt ggggatttat tagggcatcc aacggctgtc
     2461 gagaactatt atggcagaaa cgatcatcgg ccagttcagt aagtgagaca agtatcttta
     2521 ataaataaca cattttaata agtagttgaa cacataacta ccttacttgc aaaggcttaa
     2581 caatatctat acaggtacaa caaaaaactg tccaccaatt t
//
LOCUS       DAXWOO010000227         2614 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.232, whole genome shotgun sequence.
ACCESSION   DAXWOO010000227 DAXWOO010000000
VERSION     DAXWOO010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.232"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            47..484
                     /locus_tag="ACGE70_002740"
     CDS             47..484
                     /locus_tag="ACGE70_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694331.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGQWFFQALLCLEAGVKPNTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYLENVNKYGACYIMKKEDNENE"
     gene            477..1172
                     /locus_tag="ACGE70_002741"
     CDS             477..1172
                     /locus_tag="ACGE70_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694332.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEELNSKDATVDDYYLVFTKFLTCGTE
                     SIVLMGMDYELAVPEVFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNELDRK
                     RMPYQRFERKKGQ"
     gene            1337..1537
                     /locus_tag="ACGE70_002742"
     CDS             1337..1537
                     /locus_tag="ACGE70_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694333.1"
                     /translation="MNKIIPLLLVLFVGEILMRLSAAVHLNLAVFLVILMFCLIFWRF
                     VLALVSLLFKMIAAIFFIGLFR"
     gene            1553..1732
                     /locus_tag="ACGE70_002743"
                     /pseudo
     CDS             1553..1732
                     /locus_tag="ACGE70_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1794..1978
                     /locus_tag="ACGE70_002744"
                     /pseudo
     CDS             1794..1978
                     /locus_tag="ACGE70_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003027804.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            2012..2443
                     /locus_tag="ACGE70_002745"
     CDS             2012..2443
                     /locus_tag="ACGE70_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFF8694334.1"
                     /translation="MLSEPLAKTYPAIFSPEEGGGYFIEFPDVQGAYTGINEDDISYG
                     IAMAQEVLGMVLADYIEHEDLLPEPTPINKISVEDDSFTTLIRVDVAKYLKDTELVKK
                     TLTIPQWADKLGKRAGINFSVLLTESIADKADNILRSGRNN"
BASE COUNT          935 a          408 c          540 g          731 t
ORIGIN      
        1 cgaatattgg ttaaccaaat tagagaacta aaaaggagaa aaaaatatgg ctcaaaatta
       61 ctatgacgaa tttgtcaaat tacctttgga taaaatggca caaaaaatgg aggatatgac
      121 attcctatac aatgagacac gtgttccaaa aaaacattat aaggaaagat tgtcggtagc
      181 tgtggaagaa atgattgaat cgggtgtcga aatgaatctc attgcgacct actatcgaac
      241 gcttgaagaa ctgaaaaagc aaaatggaca atggtttttc caagccttac tatgcttgga
      301 agcaggcgtc aagccaaaca ctatcaaacc ttctgaatac caagctttag aactaacgta
      361 tgggaaattt gtcgaaacga aaaaagccaa gactgtttca agtgaatggt tggactactt
      421 agaaaatgtc aacaagtatg gcgcatgtta catcatgaaa aaggaggaca acgaaaatga
      481 ataaggtgat ttatcaacta acaacggaag atgtgtcaca ctttttaaca aacaaagaac
      541 atttgaatga agtaatcaga cggattcaaa aagactttag ttgtatggaa gaaatgaagt
      601 actttattca aagagtcgaa gaacaattgt atttggaaag tgaaacgagt caaacgaagg
      661 ttacagtcag caataattat ttagacgtgt ataaacgtcg tcctgacaca ttaaaaaaag
      721 gagcggagct tctacgacta gaaacagaaa aatatatcat gaatgtttac ctagaaagga
      781 gtgaactggt cgaaaacttt gtcagctaca cgatttgcag gaaacactat tactcagaat
      841 cagaatatta ttcaattttt gagagcatgg aattaatgga agagctaaac agcaaagatg
      901 cgacggtaga cgattattac cttgtattta ctaaattttt gacgtgtggg acggaatcta
      961 tcgtgttaat gggaatggat tatgaactcg ctgtacccga agtatttttg agggaatatg
     1021 tagaagaagt agataaccca ggcgacgact ggtacacgac tttaactgat tttttagata
     1081 cctacactta cgacgataca gaagaaatcc aaaatgaact tgaccgtaaa cgaatgccat
     1141 atcaacgatt tgaaaggaaa aaaggtcaat aaccatttat accgaaaagt gagaatgtgt
     1201 gaaataaaaa caaaaaacct tgaaattaag ggaatggttc ttatttcgca ccaatcgctc
     1261 caaccgcccc cgccgcttag gtggtgcgaa ataagcgagc aaagcccgaa aacctaagag
     1321 aaagaaggag atttatatga acaaaattat tcccttatta ttagtcctat ttgttggaga
     1381 aatacttatg cgacttagtg cagctgtaca tttaaacctc gcagtatttt tggtaatact
     1441 aatgttttgt ttgatctttt ggcgatttgt attagccctc gtaagtctac tattcaagat
     1501 gattgcagcc atatttttca taggattatt tcgatgaaag gagagagtac caatgccaat
     1561 tgcaatagga aataaacggt tacccgtgac gttggatgaa aagagacaaa aagaattgca
     1621 gtaaccaaag cagaagtacg gcaaaagtga atccaggatt atgtgtattg cgttagattt
     1681 attgattgcg caagaaaaag caggatttga cataccagca ctcaaaaagt gatcgcactt
     1741 tgcgcacgtg ttaatacgtg ttataattat tttgtaagga ggtatagtgc acaatgccac
     1801 ttacgggaaa agaaatgctt aagttattga aaaaaatggc tgggttgagc gaaggcaaga
     1861 gggatttcat catcatcttt ataaggatgg tgttagaatc actgttcctg ttcatgccaa
     1921 tcaagatctt ggccgaggac ttgagcgaaa aattttaaag gatgcgggat tgaaataatc
     1981 tcgcattcga ttaaagaaag gattgactga aatgttaagt gagcctttag caaaaaccta
     2041 tcctgctatt tttagcccag aagaaggtgg tgggtacttt attgaatttc ctgatgttca
     2101 aggtgcttac acaggaatta acgaagacga tatatcttat ggaattgcta tggcacaaga
     2161 ggtgttaggt atggttttag cagattatat tgagcatgaa gatctacttc cagaaccaac
     2221 accaatcaat aagatttctg ttgaagatga ttcttttact actttaattc gtgtagatgt
     2281 tgccaaatat ttgaaagaca cagaacttgt caaaaaaacc ttaacaattc ctcaatgggc
     2341 ggataagcta ggcaaaaggg ctggcataaa tttttcagtc cttctaacag aatcaattgc
     2401 agataaagca gataacattc ttcgttctgg aagaaataat tagaactcaa aagggtcaga
     2461 aacttaaaag tttttgatcc cttttttatt cctcatttca tagctttttt agtagctcaa
     2521 ttaattaggt cagaaagtta cttttttaaa aggacaccag agtataccct gttgggctct
     2581 tggtgttcta ttgaatgtaa atgagtgtca tttt
//
LOCUS       DAXWOO010000228         2581 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.233, whole genome shotgun sequence.
ACCESSION   DAXWOO010000228 DAXWOO010000000
VERSION     DAXWOO010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.233"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            57..1096
                     /locus_tag="ACGE70_002746"
     CDS             join(57..554,557..1096)
                     /locus_tag="ACGE70_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611651.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HFF8694335.1"
                     /translation="METERQIRELEKAMNTTDNRRLYERFLAVKLVLEGHTRKEAGHT
                     IGRDEHTVSHYVKNYCHNGIQGLVSKKQSGRPSRLTDEQEAELVQIVAYHTPEEVGFK
                     SRANWTLAIVCDLILREWSYGYTQKGVADLLHRLGLSWTRPTYTLKKADSKKQQVFLE
                     ETFPSLKKLLNSDIDHLLFEDETMIRDYQAIGCTWFLRGHQRKIPTYGQHKGVKLIGT
                     LDYETGRIICTEEGAYDAAAFLRFLERTLREYPTGKIVMVLDNALIHHAKLIQSFLEE
                     NARRLALIFLPPYSPQLNLMEGVWKWLKESVINNVFFDHVQKIKQSVRGFLADVNERP
                     LEVIDRLCVRM"
     gene            1109..2047
                     /locus_tag="ACGE70_002747"
                     /pseudo
     CDS             1109..2047
                     /locus_tag="ACGE70_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
     gene            2040..2387
                     /locus_tag="ACGE70_002748"
     CDS             2040..2387
                     /locus_tag="ACGE70_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694336.1"
                     /translation="MIEYNDRGRLKWSGFYLSDHTERINADNVQRANHNLAKEQMTTE
                     EIAKILRTAVIKNRPVCIQKEERNAEGYYPDDVVGKIIGYDELGVFVEEEKVHYDEIR
                     NVSFSELLKWSQI"
     gene            2384..2566
                     /locus_tag="ACGE70_002749"
     CDS             2384..2566
                     /locus_tag="ACGE70_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694337.1"
                     /translation="MNIIKKYEFGYITYEQLIEELWGYGQQLINEVGIDCFCFYIESG
                     AGYHKNRYYIFPYPPK"
BASE COUNT          886 a          471 c          580 g          644 t
ORIGIN      
        1 attcgttttt cctcctatag gcgtatacta aagaaaacac ttgaggagtg gggaacatgg
       61 aaacagaacg ccaaatccgt gaattggaaa aagcgatgaa tacgacagac aaccgccggt
      121 tgtacgagcg tttcctggca gtcaagttgg ttcttgaagg ccacacccgg aaagaagccg
      181 ggcacaccat cggccgcgac gagcatactg tcagccacta tgtgaaaaac tactgccata
      241 atggaatcca aggtttagtc tccaaaaaac aaagcggacg accttcacgg ctgacagatg
      301 aacaggaagc ggaactggtc caaatcgtcg cctaccacac tccggaggaa gtaggtttca
      361 aatcccgtgc caattggaca ttggcgattg tgtgtgacct gattctccgg gaatggagtt
      421 atggatatac gcaaaaaggc gtggcggacc tccttcaccg gctaggacta agctggaccc
      481 gtccgacgta taccctcaaa aaagcggatt ccaagaaaca acaggtattt ttggaagaaa
      541 ccttcccgag cctaaaaaaa aattgctgaa tagcgatatt gaccacctcc tattcgagga
      601 cgaaaccatg atccgggatt accaagccat tggctgcacc tggtttctcc gcggccacca
      661 gcgaaaaatc cccacttatg gacaacacaa aggggtaaaa ttgataggca ccttggatta
      721 tgaaaccggt cggatcattt gtacagaaga aggggcatac gacgcagcgg ccttcctccg
      781 ttttctggag cggacgttgc gggaataccc caccggaaaa atcgtgatgg tattggataa
      841 tgcccttatc caccacgcca agctgatcca gtcttttctt gaagaaaacg ctaggcgcct
      901 ggcactcatc ttcctccctc cctacagccc tcagttgaac ctaatggaag gcgtttggaa
      961 gtggctcaag gaatcggtca tcaacaatgt tttttttgac cacgtccaga aaatcaaaca
     1021 gtcggtccgc ggtttcttag ccgatgtaaa cgagcgtccc cttgaggtga ttgaccgact
     1081 gtgcgtgcgg atgtaaagta tttaattcaa cctatatagt tcaggaacaa gaagcgagag
     1141 aagaagagaa ttagctaaaa taatcaaaga tgagattcag aaagaaacag gtctaatagc
     1201 aacagtcgga ataggagata acccattatt agctaaactt gcactggaca atgaagcgaa
     1261 gagtagtaaa gaatttatag ctgagtggac ttatgagact gtacctgaaa aaatatggaa
     1321 aataaacgag atgactgact tttggggcat tggatcacgc atgaaaaaaa gattgaatca
     1381 tatgggaatt atgagtatag aagatttagc aaattggaat ccctacacaa ttaaaagtca
     1441 gttaggtgta atcggactcc agttatattt ccataccaat ggaattgatc gtacggatat
     1501 tgctattcca ccagaaccaa ccaaagaaaa gtcttatggg aattcacaag ttctaccaaa
     1561 agattatacg aggagagaag aagtagaact agtagtcaag gagatggcag agcaagtagc
     1621 cattcgaatt agacaacaca attgcaaaac tggttgtgta cgcttagcaa ttggtacgtc
     1681 aattttggaa gaaaaatcag gtttctcgca ccagatgaaa ataccaatta cagataatac
     1741 gaaagaatta caaaattatt gtcttttttt attcaataag ttttatgagg ggcaggaagt
     1801 acgacatata ggagtaactt atagcagatt agtatacaca gattccctac aattagattt
     1861 atttagtgat ccaagaaaaa agattgctga agaaaatttg gataggatta ttgataagat
     1921 tagacaaaaa tatggattta cagctattat acatgcttca tctatgttag aaggtgcaag
     1981 aagtattacg agaagcactt taataggagg acacgcagga ggattaggag gtttgcataa
     2041 tgattgaata taacgataga ggacgtttaa aatggtcggg attttatttg tctgatcata
     2101 ctgaaagaat caatgcagac aatgtacaac gtgctaatca taatttggca aaagaacaaa
     2161 tgacgactga agagattgcg aaaatactga gaactgctgt cataaaaaat agacccgtct
     2221 gcattcaaaa agaggaacga aacgctgaag gttactatcc agatgatgta gtaggaaaaa
     2281 ttattggata tgacgagctt ggtgtatttg tggaagaaga aaaagttcac tatgatgaaa
     2341 ttaggaatgt ttctttctcg gaattattaa aatggagtca gatatgaaca ttattaaaaa
     2401 atacgaattc ggttatatta cgtatgaaca gctaatagag gaattatggg gatatggtca
     2461 acaattaatt aatgaagtgg ggattgattg cttttgtttc tatattgaat ccggagcagg
     2521 ataccataag aacagatact atatatttcc atatccacca aaatagtctc ttagatattt
     2581 t
//
LOCUS       DAXWOO010000229         2571 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.234, whole genome shotgun sequence.
ACCESSION   DAXWOO010000229 DAXWOO010000000
VERSION     DAXWOO010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.234"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(69..971)
                     /locus_tag="ACGE70_002750"
     CDS             complement(69..971)
                     /locus_tag="ACGE70_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFF8694338.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(968..1660)
                     /locus_tag="ACGE70_002751"
     CDS             complement(968..1660)
                     /locus_tag="ACGE70_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFF8694339.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(1703..2344)
                     /locus_tag="ACGE70_002752"
     CDS             complement(1703..2344)
                     /locus_tag="ACGE70_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFF8694340.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(2382..>2571)
                     /locus_tag="ACGE70_002753"
     CDS             complement(2382..>2571)
                     /locus_tag="ACGE70_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694341.1"
                     /translation="NFYRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGA
                     VSKMVKSGNVANISYLMI"
BASE COUNT          711 a          573 c          423 g          864 t
ORIGIN      
        1 aaaattctgt cacaacccct cttttttgat caacggtgtt caataaacag cgttctcatc
       61 tgcttttctt agctctgctg gtccagtaac cgagtaagct ctttgataaa tctttgagcg
      121 atttcactga gcggacgttt cgccgaataa atgatataaa attcatttag ttcgccttct
      181 gccaaaggga ttaaccgcaa gtgctcgcga atccctccaa tcacaatacc agatcctgaa
      241 gcgtaagcat cagtttcaag aacgatattc atcaaagtac ctcgatcact cgtataaatc
      301 actgtttctt gatcagggat ctcgataagg tcttcataaa aatacgggaa attatttcca
      361 tcctgtgtaa aacgaacttg aggataagat aacaattctt ctttggtgat ctctgcgtgt
      421 tcagctaatg gatgtccttg ccttaaaaaa atatgcgttt tgaattttcc aatgctgcgg
      481 ccacttagct ctccttgttg taaatatctt ctgataccag cttgattatg ctcattcata
      541 taaataatgc cgatctcact gtgctgctct ttgacttctt cgatcactct cgctgttgtc
      601 gtctccatga tccgaaattg tttgatctcg tctttgaatt cctcgagtag ctgtgcggtc
      661 aaaggaccta agaaatcata atgctgggca gcgatcgaaa aattgtctct cttttcactc
      721 gttagatagt gagtttctaa tgtatcaagc tggaccaata ttttctcggc acgatttaag
      781 aagtcttgcc cttcttctgt taaatatgcc cctcgattcg ttcgagtaaa cagcgtgatc
      841 cctaactcgg tttctaattc tttgatacca gctgaaagac ttggctggga gacaaacatt
      901 ttctttgccg cttctctgaa actaccattg tttgctacag cgaccacgta tttcatctgc
      961 tgaatattca ttgctcaaac tcctcgaaat aataaatttc tcttccattc aagggctgga
     1021 ttggtcggtt gttcggtcga gcatactgac catcgaaggg gagaagaaac tcttggttca
     1081 gttttcctac atgatccatt acaaaccatt ggattggccc gctggtaggc ggagttgtca
     1141 atgagccaat ataatggaaa tggctttttt gctccggaaa gaacacatcc ggatttaccc
     1201 aatgctcatg tttttctaag ttccaacttt ccccattttc ttgattatac agccaacctt
     1261 cctgtaccat tagctggtac atgacaccca caactagatt ctcttttttg ctgttcatat
     1321 gaacaaaatg aaattcaata tcttcctgtt ttccgttgat gatatgttca gaaggcaagt
     1381 ggaaatgaat atctgtcaaa taatagcgtt ctttcttgaa ctctacataa ctgagttggt
     1441 caaaaggtac taaatgaatc gtgtttttga attccttgtc agtaaaccgt tgcttttgat
     1501 aatgaaagga aagcgtgtct ccttctattt tggtttcttc ccccttggtc aaatgaatag
     1561 gcgactgcaa tggaaattcc gcacctctcg caaaccaatc acataaactc gcccaatttt
     1621 ccggaccttt gtctccagaa tagccccatt cgacatccat attctttttc aactatactt
     1681 ccccctgcat ctcatatcat ttttattttt cttctttctt tacaatcgtt gagacggttt
     1741 ctaaaaacgc ctcttttcca taaaattcct ggttcaatag ttccggaaat atctctttaa
     1801 agaaatactg aacagaaggc cgatgttcaa agcttttaat cgtagtcact gcctcaatga
     1861 acatctcctt ataaactttt tccgggttat ttttgatgat ttcatcaaaa gattcataaa
     1921 gaaggtcaac cttatctgaa atggctaata gttctccttc tacggtttca tcttttcctt
     1981 cgaacaaccg acggcgataa acttcttgga actcagctgg aatctcttgg ttgatgaatt
     2041 cgtccgtcat cttttcttca actgtttgca gcatccctct taactctttg cttgcgtatt
     2101 tcaccggcgt tttgatatca ccaataaaac gctctgtgta gtcatgattc aatgattttt
     2161 catagagcag tttccaatcg atcggttgtc cattcgcttc ttcaatgtct cctagaactt
     2221 gcgcaataga tgctacacga tacgaatgtg ctgcaactgt atgctctgta tatttaaaaa
     2281 atccaggtgc ccttgtgatc gtctctaaat tgttcaatcc taaaataaat tcattcaacc
     2341 ccataaaagt tacctccctt tgagatttct ttttccgaat atcatatcat taaataactg
     2401 atattcgcaa catttccgct ctttaccatt tttgaaacag ctcccttacc aaaaaataga
     2461 gaaaatctat atataataga aaaaagagaa gagattattc tcttctttct gctaaccagt
     2521 cttagaaaaa tgaattttaa aaaaatcaaa aactgatcac gatagaagtt t
//
LOCUS       DAXWOO010000230         2545 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.236, whole genome shotgun sequence.
ACCESSION   DAXWOO010000230 DAXWOO010000000
VERSION     DAXWOO010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.236"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..344
                     /locus_tag="ACGE70_002754"
     CDS             <1..344
                     /locus_tag="ACGE70_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFF8694342.1"
                     /translation="KSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAP
                     IILADEPTGNLDSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKL
                     KKVNKMNLEVE"
     gene            344..1719
                     /locus_tag="ACGE70_002755"
                     /pseudo
     CDS             344..1719
                     /locus_tag="ACGE70_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            1849..2262
                     /locus_tag="ACGE70_002756"
     CDS             1849..2262
                     /locus_tag="ACGE70_002756"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFF8694343.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIADCRQKLFQTRNSSLEQLFIYFVSNF"
     gene            complement(2287..2545)
                     /locus_tag="ACGE70_002757"
                     /pseudo
     CDS             complement(2287..2545)
                     /locus_tag="ACGE70_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012008915.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          939 a          355 c          460 g          791 t
ORIGIN      
        1 ataaatcagc agatgaaagt atcttgtttg aactaggttt agataaaaaa caaataaaaa
       61 gaaatgttat gaaattatct ggtggacagc aacaaagagt agctattgct agagcattgg
      121 tatcagatgc tccaataata ctagctgatg agcctactgg taatctagac agtgttactg
      181 ctggagaaat aattaatata ttaaagacat tagctaaaga tagaaataaa tgtgtaatag
      241 ttgtaacaca tagtaaggaa gtagcagatt ctgcggatat cattttagaa ctaagtggta
      301 aaaagttgaa aaaagtaaat aaaatgaatt tggaggttga ataatgataa aaaatgcatt
      361 tgcttatgta actagaaaaa gtttaaaatc attaattatc atattagtta ttttatctat
      421 gtcaacttta agtctcatta gtttatctat taaagatgct acggacagag cttcaaaaga
      481 aacatttgct aatataacaa atagtttttc tatggagata aatagacaag taaatccagg
      541 aacacctaga ggtgggggaa atgtaaaagg tgaagatatt aaaaagatat ctcaaacaga
      601 tagtatagac tcctatgtaa aaagaataaa cagtgttgca gatttagttg attatgatat
      661 tattgaaact caagagactc ttgcgaatca atcacctgaa agagcaaaga attttaaaag
      721 aacagttatg ttaactgggg ttaacgactc aacaaaagaa acaaaatttg tatcagaagc
      781 atataaatta gtagaaggaa agcatttaga aaatgaagat aaaaataaaa tcttaatgca
      841 taaagattta gctgaaaaaa ataaccttaa agttggagat aagataaaaa taaaatctaa
      901 tttatttgat gctgataatg agaaaggtgc agatgaaaca gtagaagtag aaattaaagg
      961 tctattcgat gggcataata gcggtgtagt aagtgcagca caagaactat acgaaaatac
     1021 attaatcact gatattcata cagctgctaa agtttatggt aataccgaag atacagctgt
     1081 ataccaagat gcaacattct ttgtaaaagg tgataagaat cttgatagtg taataaaaga
     1141 tcttgggaaa ttagatataa attggagaga atataatttg attaaaagtt catcaaatta
     1201 ccctgcatta caacaatcta tatcaggtat ttactcaatt tcaaataaat tattttttgg
     1261 ctcattaata tttgcaggag ttgtagtttc attattatta ttcttatgga tgaatgctag
     1321 aaagaaagaa atagcagtat tactatcatt aggtatatca aaattagaaa tttttggtca
     1381 attcctaatt gagatggtat ttatatcaat ccccgcattc gttggttcct atttcttagc
     1441 tcaatataca gctgacaagc ttgggaataa tatattgaat aaggttactg gtgatatagc
     1501 taaacaaata gctaaacaat ctgcatctag ccaattaggt ggtggggcag aagctgaagg
     1561 atttaacaag acattatcaa gtttagatat caatgtatta cctaaattca tgatttatgt
     1621 agttatattt tgagtttagt acttcttgta tcattgatta tttcttcatt caatatatta
     1681 agaaggaatc caaaagaatt attaattgac aataattaaa attttcggtg ctttttagca
     1741 ccaaaaatga gagtgtaata taaaccgtgt aagtaaccat caatcctagg cgggtggttg
     1801 cttcacggtt tttatttttc taaaataaga tcaagaggtg attacacgat ggctagaaag
     1861 aaaagaaatc ctgatgctga gaagttagct gaatccattt tgaatactta tcaacccgaa
     1921 tctgtagagg atatgcaaga tgttttaaaa gatgtgtttg gtcccttatt tgaaaaaatg
     1981 cttcaaggag aattgaataa ccatttaggg tatgacgccc attctaaaga gccaaagaaa
     2041 catgacaatc gacgaaacgg ttatggcaat aagacgctta aaacgagctt tggtaaagta
     2101 aacattgatg ttcccagaga ccgggaggct tcttttgaac ctgagttgat tcctaataga
     2161 aaacgagatg tctccgacat cgcagactgt agacaaaagc tgttccaaac tcgtaactcg
     2221 agcttggaac agctttttat ctacttcgtt tcgaattttt gaacaaaaaa ataaaggctc
     2281 cttgcctttc catagccagg tggctaattt ctttaaattc attgcagcaa acactagcat
     2341 cgtatgcgtc gcgacttttt ccaagcctcg atacttggtc caacgcatcc catgcttttc
     2401 ttttgcatcc gcaaatattc gttcaatcgt ttgtttccgt tttttgtaaa ttgatttatt
     2461 cagatctgtt aggcgaagat gttccacttc atccatcaga tctccccaaa catgtctcgt
     2521 gatgactcgt tgatgatttt tactt
//
LOCUS       DAXWOO010000231         2506 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.237, whole genome shotgun sequence.
ACCESSION   DAXWOO010000231 DAXWOO010000000
VERSION     DAXWOO010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.237"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..602
                     /locus_tag="ACGE70_002758"
     CDS             <1..602
                     /locus_tag="ACGE70_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFF8694344.1"
                     /translation="LPTLYSLLCVMAPCIIYMVFFCKKNTLKHNILILIFMLYCWKVF
                     ELTGTGGLTDIFYRPDTGRKFSLVQGSVNLVPFSGLNISFILNIVMCVPLGFLLPIIW
                     KSYRKIVPVFLAGALFSLIIEVSQIITTRATDIDDLIANTFGAVIGFILWAVLSRVVS
                     LKDKSSTVDYLEPYIYILIGFTGKFLLYFPFWFAFEVLS"
     gene            675..1604
                     /locus_tag="ACGE70_002759"
     CDS             675..1604
                     /locus_tag="ACGE70_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HFF8694345.1"
                     /translation="MKRFLKWGLVLLASLIVILMCTAVVISYVNRPYARAVYDRIKRI
                     LDKDFYDHLYLGSSFEIINLDTMDIHSYMKKDQEELPASLSKLFTIDYALSVLSLDEQ
                     ITISPTALDMQKADSSTAHLQEGDILSVYDIICAMVLPSGNDAAYSLAIYTGGKLDPD
                     AKNDEERYQSFMKNLDRYLSEQGYKHTHFTDAAGYDSNATTSAEDLTKVAAKLLKNDK
                     FAEIVAKETYRAKSIKGNVYNWNTTNQFMQQDNRYFTEGVKGVKTGSFDGRYNLCVYY
                     TSSSDNHYLLVSFGSESDSRRYVDVQNLLHNLP"
     gene            1741..2499
                     /locus_tag="ACGE70_002760"
                     /pseudo
     CDS             1741..2499
                     /locus_tag="ACGE70_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033645.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
BASE COUNT          869 a          358 c          469 g          810 t
ORIGIN      
        1 tactacctac actttatagc cttttatgtg taatggcacc atgtattatt tatatggtat
       61 ttttttgcaa aaaaaatacc ctgaaacaca atatcctgat tttgattttt atgctgtact
      121 gttggaaagt ttttgaattg actggtacag gtggactaac agatattttt tatagacctg
      181 atacaggtag gaaattttct ctagtacagg gcagtgttaa tcttgtacca ttcagtggac
      241 taaacattag ctttatatta aatatagtaa tgtgtgtgcc cttaggattc ttactaccaa
      301 tcatatggaa aagttatagg aaaatagttc ctgttttctt ggctggggct ctattttcac
      361 taattattga agtctcacaa attataacta caagagcaac agatattgat gatttaatag
      421 caaatacctt tggtgcggtt ataggcttta tactgtgggc tgttttaagt agagtggtgt
      481 ccttgaagga caagtcatct acagtagatt atttagaacc ttatatttat atattgattg
      541 gttttaccgg aaaattctta ctgtattttc ctttctggtt tgcttttgaa gtattatcct
      601 gataattcca tagaaaataa tgagtttgag ttgagaaaga atattcttgt acacaactct
      661 ggaaaggagg aagtatgaaa agattcttaa aatgggggct tgttctttta gcgtctttga
      721 ttgtgattct aatgtgtaca gcggttgtta tttcttatgt gaatcggccg tatgcaagag
      781 ctgtttatga taggattaaa cgcattcttg acaaagattt ttatgaccat ctatatctag
      841 gttcttcatt tgaaataata aaccttgata caatggatat tcatagctat atgaaaaaag
      901 accaagaaga attacccgcc tcattgagca aattatttac gattgattat gcgcttagtg
      961 tcctaagttt agatgaacag attacaatta gtcctactgc acttgacatg cagaaagctg
     1021 actcatctac tgcacatctt caagaaggag atattttatc tgtttatgac attatctgtg
     1081 ctatggtctt accctcagga aatgatgcag cttatagttt ggcaatttat acaggtggaa
     1141 aacttgatcc tgatgcaaag aatgatgaag aacgctatca aagcttcatg aaaaacttag
     1201 atcgttatct aagtgagcaa ggctataaac atactcattt tactgacgct gctggctatg
     1261 actccaatgc tacaacatca gcggaagatt tgacaaaagt agctgcaaag ctactaaaga
     1321 atgataaatt tgcagaaata gttgccaagg aaacttatag agcaaaatca attaagggaa
     1381 atgtttataa ctggaatact acgaatcaat ttatgcaaca agataataga tactttactg
     1441 aaggtgtaaa aggtgtaaaa actggatcgt ttgatgggag atacaatcta tgtgtgtatt
     1501 atacatctag ttctgataac cactatttac ttgtaagttt tggttcagag agtgatagta
     1561 ggcgttatgt agatgttcaa aatctattgc ataatctgcc ttgaaatttc gtgcctttaa
     1621 gcgcgtatct aaagtttctt tgaaaagttt gagaatgaag aaagtgagca gtcgaaaaac
     1681 ggttgctcac ttttttgccc taaaatcgaa aaaatataaa aaagtccttt acaacacgtc
     1741 tcaggaaaaa ctgctaagtc aattctaata tcttgtggag caagacttgc accttttgat
     1801 ataagagagc ttagagaact aatgagcgaa gatgaactag aactcgataa aattggagat
     1861 agaaaaactg ccttgtttgt aataatatca gatacagatg atacatttaa ttttgtagtt
     1921 tcaataatgt attctcaatt atttaatcta ctatgtgata aggcagatga tgtgtatggt
     1981 ggaagacttc cagttcatgt taggtgtcta cttgatgagt ttgcaaatat cggtttgatt
     2041 cctaaatttg aaaaactaat agcgacaatt cgttcaagag aaatatcggc aagtataata
     2101 ctgcaagcac aatctcaatt aaaagcaatt tataaagacc atgcagatac aatagttggt
     2161 aactgtgact ctacactttt tctaggagga aaagaaaaaa caacagtaaa agaattatca
     2221 gaaaccttag gaaaagaaac catagaccta tataacacat cagaaacaag atcaaatcaa
     2281 aaatcttttg gtctaaatta tcaaaagact ggcaaggaac ttatgagtca agatgaaata
     2341 actgtaatgg atggtggtaa gtgtatttac cagctaagag gagcaagacc ttttctatct
     2401 gataaatttg atattacaaa gcataagaat tacaaattgt tggaagatta tgataagaaa
     2461 aacttgtttg atgtagaaga gtatttaaaa caaagatagc gtaaaa
//
LOCUS       DAXWOO010000232         2499 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.238, whole genome shotgun sequence.
ACCESSION   DAXWOO010000232 DAXWOO010000000
VERSION     DAXWOO010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.238"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            12..494
                     /locus_tag="ACGE70_002761"
     CDS             12..494
                     /locus_tag="ACGE70_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFF8694346.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            531..1541
                     /locus_tag="ACGE70_002762"
     CDS             531..1541
                     /locus_tag="ACGE70_002762"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFF8694347.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(1656..2369)
                     /locus_tag="ACGE70_002763"
     CDS             complement(1656..2369)
                     /locus_tag="ACGE70_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694348.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT          852 a          408 c          512 g          727 t
ORIGIN      
        1 gagacataaa aatgattgac ttggcaaaga aattcttaca cgaaaatata tattttgagt
       61 tgtatcatca tcgagccatt tatacaaatg aagatgcttt ggtaataaag aaagaacaag
      121 gatttacagg tacagagacc aaaagcttat tcttaaaaga taaaaaagat aataactacg
      181 tatatctgac ttttactacg aaacaaacgg atttcaaagc attagggaag atcgtcggta
      241 aaaggctttc tattgtttca tcagaacgca tggaagaaca aacgggacaa aagcagggcg
      301 ctgtttctcc gtttggttat gaaaaacaga ttccgattat tattgatgaa gagctcttat
      361 caaatgaaaa acttgttttt gcaccaggta gaccagatca aacgatggtc atccaaacgg
      421 ttgacttaaa caaaatcatc cagctacttg agatgccgat atttttgatg ccggcaaacg
      481 aaaaaaacaa ttaaccaagt aaaatagtgc atacttggag gtgaagcaac atgataagtt
      541 atccattatt agaaagaaat gcaacgattg gggttactgc accatcctca ggcgtatcag
      601 aggaacacca ttcgttactc gaatcagctt taaagagaat ggaagaaaaa tctttccaag
      661 tagttactgg aaagacgctt tggacgcaag ataaggaaaa atcaggtcca gcaagaaaac
      721 gagcagatga attcaatcaa atgatgcaag atgaaaatat ccaaatgatc tttccgcctt
      781 ggggaggaga attattgatt gagattctcg attttgtcga ttttgatgct ctacaaccaa
      841 aatggatagt aggttattca gatattagta cgttgctact tgctgtcaca ttaaaaacag
      901 ggatagccac tgcacatgga accaacctta ttgatttaag aggagagtac ttggatgaaa
      961 cgacagcaat gtgggaaagt gtactgaaaa caagagaagg agaatcgatt ctacagcaac
     1021 cttctgaaaa gtatcaaaaa gagtggcccc atgagcattc atcatcttct gttttccatc
     1081 tcactgaacc aacaaattgg caatcagtca gcggagatgc agtaaggatg aagggacgat
     1141 tattaggtgg ctgtattgat acgatcagtc atttaatcgg taccccttat ggagatgtcc
     1201 aatcttttaa aagaaagtat aatgatggaa agccgatctt gtggtttcta gaaaattgtg
     1261 agctatcgtc agttgattta agaagatcac ttgttcagat gaaactggca ggatggtttg
     1321 atggcagctc ggggatcatg ttcggtagaa gtgcagcaga ggatttgtta gattataaaa
     1381 tgaaggatgt atatgaagat ctgtcaaagg aattaaaact accgatcttg tatgatattg
     1441 attgtggtca tgtaccgcca caactgacat tgatcaatgg tgcctatgca gaagtagcgg
     1501 tagaagatag aaaagcgact gttttgcaaa cgtttattta aaaaattttt tcctgccttg
     1561 gagtttatca aagaccaaca aatctaaata gaaaagaaaa tgaaagaagc tgacagtatt
     1621 ttgccagctt ctttttgtat gatctattag aaattttaat tatccataac tatcacattg
     1681 atcagctgtt tcacatcatt tggattacct gtaggaatag ctactacaga tgcattatca
     1741 ataatttctt gagttgtttc tctaatgaac gctggataag atgtttgatc tgcaggcatt
     1801 tgccaatgtg aaggaactgg ataactagtg attgtcccgt catggttact tgaatacact
     1861 aaaactccct ggtaactgta tttcccagtg agcataacca catctttcgg atagactgca
     1921 ctccctgcat cataaggggc gataggtgtt cctgcaggat aatgaataac atgtagttcg
     1981 aaatcacccg atgtatctct aaaagtatta cctctaattg ctagccaaac acgtgcgtac
     2041 tcaatttgta gatcagaata tcctgctaac ggatctattg actgagaaga ctgtgaggaa
     2101 gttgtgtcta ttgttgaaga agaaatattt gtctcaactg tactactaga tgtagaagaa
     2161 gtattgggct ctgagacttt actgcttgtt gaagatgtat tcgtttcagt agaagctgat
     2221 ttctgagtac ttgatttttc agctgaactg gtttgtttga ttgaactttt gtctgtggtc
     2281 gcagatggat cactattttc tttactagga gacgtacatc ctacaaggaa taaagagacc
     2341 aacaataaaa taaaacctaa ctttttcata taaaatacct cattattttt ttaataccag
     2401 tatagcataa tcctgaattt ttgaatccgt aaaaccttgt ctatggatag gctacaatta
     2461 actagacaga aaaattaagg tgtgtagact agaagaaaa
//
LOCUS       DAXWOO010000233         2474 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.239, whole genome shotgun sequence.
ACCESSION   DAXWOO010000233 DAXWOO010000000
VERSION     DAXWOO010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.239"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..418
                     /locus_tag="ACGE70_002764"
     CDS             <1..418
                     /locus_tag="ACGE70_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFF8694349.1"
                     /translation="QEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLD
                     LSEDNPYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMN
                     YAVKLYKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            632..1327
                     /gene="vanR-A"
                     /locus_tag="ACGE70_002765"
     CDS             632..1327
                     /gene="vanR-A"
                     /locus_tag="ACGE70_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFF8694350.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1305..2459
                     /locus_tag="ACGE70_002766"
     CDS             1305..2459
                     /locus_tag="ACGE70_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFF8694351.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          854 a          443 c          521 g          656 t
ORIGIN      
        1 acaggaagat gacatcattt atgttacaga cttaactcga atcactcgta gtacacaaga
       61 tctatttgaa ttaatcgata acatacgaga taaaaaggca agtttaaaat cactaaaaga
      121 tacatggctt gatttatcag aagataatcc atacagccaa ttcttaatta ctgtaatggc
      181 tggtgttaac caattagagc gagatcttat tcggatgaga caacgtgaag ggattgaatt
      241 ggctaagaaa gaaggaaagt ttaaaggtcg attaaagaag tatcataaaa atcacgcagg
      301 aatgaattat gcggtaaagc tatataaaga aggaaatatg actgtaaatc aaatttgtga
      361 aattactaat gtatctaggg cttcattata caggaaatta tcagaagtga ataattagcc
      421 attctgtatt ccgctaatgg gcaatatttt taaagaagaa aaggaaacta taaaatatta
      481 acagcctcct agcgatgccg aaaagccctt tgataaaaaa agaatcatca tcttaagaaa
      541 ttcttagtca tttattatgt aaatgcttat aaattcggcc ctataatctg ataaattatt
      601 aagggcaaac ttatgtgaaa gggtgataac tatgagcgat aaaatactta ttgtggatga
      661 tgaacatgaa attgccgatt tggttgaatt atacttaaaa aacgagaatt atacggtttt
      721 caaatactat accgccaaag aagcattgga atgtatagac aagtctgaga ttgaccttgc
      781 catattggac atcatgcttc ccggcacaag cggccttact atctgtcaaa aaataaggga
      841 caagcacacc tatccgatta tcatgctgac cgggaaagat acagaggtag ataaaattac
      901 agggttaaca atcggcgcgg atgattatat aacgaagccc tttcgcccac tggagttaat
      961 tgctcgggta aaggcccagt tgcgccgata caaaaaattc agtggagtaa aggagcagaa
     1021 cgaaaatgtt atcgtccact ccggccttgt cattaatgtt aacacccatg agtgttatct
     1081 gaacgagaag cagttatccc ttactcccac cgagttttca atactgcgaa tcctctgtga
     1141 aaacaagggg aatgtggtta gctccgagct gctatttcat gagatatggg gcgacgaata
     1201 tttcagcaag agcaacaaca ccatcaccgt gcatatccgg catttgcgcg aaaaaatgaa
     1261 cgacaccatt gataatccga aatatataaa aacggtatgg ggggttggtt ataaaattga
     1321 aaaataaaaa aaacgactat tccaaactag aacgaaaact ttacatgtat atcgttgcaa
     1381 ttgttgtggt agcaattgta ttcgtgttgt atattcgttc aatgatccga gggaaacttg
     1441 gggattggat cttaagtatt ttggaaaaca aatatgactt aaatcacctg gacgcgatga
     1501 aattatatca atattccata cggaacaata tagatatctt tatttatgtg gcgattgtca
     1561 ttagtattct tattctatgt cgcgtcatgc tttcaaaatt cgcaaaatac tttgacgaga
     1621 taaataccgg cattgatgta cttattcaga acgaagataa acaaattgag ctttctgcgg
     1681 aaatggatgt tatggaacaa aagctcaaca cattaaaacg gactctggaa aagcgagagc
     1741 aggatgcaaa gctggccgaa caaagaaaaa atgacgttgt tatgtacttg gcgcacgata
     1801 ttaaaacgcc ccttacatcc attatcggtt atttgagcct gcttgacgag gctccagaca
     1861 tgccggtaga tcaaaaggca aagtatgtgc atatcacgtt ggacaaagcg tatcgactcg
     1921 aacagctaat cgacgagttt tttgagatta cacggtataa cctacaaacg ataacgctaa
     1981 caaaaacgca catagaccta tactatatgc tggtgcagat gaccgatgaa ttttatcctc
     2041 agctttccgc acatggaaaa caggcggtta ttcacgcccc cgaggatctg accgtgtccg
     2101 gcgaccctga taaactcgcg agagtcttta acaacatttt gaaaaacgcc gctgcataca
     2161 gtgaggataa cagcatcatt gacattaccg cgggcctctc cggggatgtg gtgtcaatcg
     2221 aattcaagaa cactggaagc atcccaaaag ataagctagc tgccatattt gaaaagttct
     2281 ataggctgga caatgctcgt tcttccgata cgggtggcgc gggacttgga ttggcgattg
     2341 caaaagaaat tattgttcag catggagggc agatttacgc ggaaagcaat gataactata
     2401 cgacgtttag ggtagagctt ccagcgatgc cagacttggt tgataaaagg aggtcctaag
     2461 agatgtatat aatt
//
LOCUS       DAXWOO010000234         2460 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.240, whole genome shotgun sequence.
ACCESSION   DAXWOO010000234 DAXWOO010000000
VERSION     DAXWOO010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.240"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(561..1901)
                     /gene="glnA"
                     /locus_tag="ACGE70_002767"
     CDS             complement(561..1901)
                     /gene="glnA"
                     /locus_tag="ACGE70_002767"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFF8694352.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(1929..2321)
                     /locus_tag="ACGE70_002768"
     CDS             complement(1929..2321)
                     /locus_tag="ACGE70_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFF8694353.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT          723 a          543 c          418 g          776 t
ORIGIN      
        1 tcacataagt aaaaaataag gagatgcgac aaaagtcgtg cctccttatt tctaaataaa
       61 cggtgttctc ctgccaactc ctcggtcaca agtcacaata tccatcaaac atgagaagct
      121 gtttttgaaa ttatttcttg tgattcggag ttaaatggta ggctcacaac ctctggataa
      181 acggtattca aaagccagtt tctcggtatt aagccaaatt tatgcaaaaa tgtagaaagc
      241 cattttcaca aaatttttct taatactcga aactaaacgt ctttttcaaa acctcttggt
      301 aaacggtgtt caaaagcttg catctgcggt aataagttca gccaattcaa aaacaagaaa
      361 agctgttttt gcttggctat tcttattact tgttgcaggg ctgctttttc acaacctctc
      421 cactaaacgg tgttcaatca gctgaccctc ggtattagct caaaaaacgg caaaatatga
      481 ggaactattt ttccgttagt ttctctaata ctcaggtcaa aacgctgatt tcacagcctc
      541 taattcacgg tattcctgtt ttaataaagt tctagatatt gttctctttc ccactcggaa
      601 acagtttgtc tgaatgccgc ccattcgaga cgttttgctt caacaaaatt tgagaagata
      661 tgttgtccta atgcgtcaat cattacatta tccttacgca attctttgat agcattatgg
      721 atagttgaag gtaagtcctg gatatgtgca gcttctcttt cttcctcatc cattacataa
      781 atgttacggt caacagctgc tggcggtgtg atctgattac ggataccgtc tagtcctgct
      841 tgcaacaata ctgccattgc caagtatggg tttgcagatg gatcaactga ccgcaattcc
      901 aaacgtgtgg ataaaccgcg agattctggt acacgtacta atggcgaacg attacggcca
      961 ctccatgcga cataaacagg cgcttcataa cctggaacga gacgtttata agaattgact
     1021 gttggattgc aaacggctgt gtaggcacga gcatgtttca gcaaaccacc taagaaatga
     1081 taagctgttt cacttagttc caattcaccg tctttatcat agaaaacatt tccttcatca
     1141 ttgaacaaag acatattgca atgcatacca gaaccattga tcccaaataa cggtttcggc
     1201 atgaaagtcg cgtgaagtcc atgcttgcgt gcaatcgttt tgacaacgag cttgaaggtt
     1261 tggatattat cacaagcatc taccacatct gcatatttaa agtcaatttc atgctgtcct
     1321 ggcgctactt catgatgtga agcttccact tcaaagccta gactttctaa ttccaacacg
     1381 atgtcacggc ggcaattttc acctaaatct gttggtgcaa aatcaaaata accaccttta
     1441 tcattcaagg tcgttgtgat cccaccgtct tcgtctaatt taaataagaa gaattctggt
     1501 tcaggtccta aattgaaaga ggtaaaacca aggtcgcgca tatctttcaa tgctcgtttc
     1561 aaattcccgc gaggatcgcc ggcaaatggt gtcccatccg gattatagat atcacaaatt
     1621 aaacgagcaa cttttccatg agcactttcc cacgggaaga tcatccaagt ggagagatct
     1681 ggatacaaat acatatcgct ttcttcaatt cgaacaaatc cttcaatcga agaaccgtca
     1741 aacatcattt tattttctaa tactttgtcg atctggctca ctggaacttc tacatttttg
     1801 atcgtaccta agatgtctgt gaacatcaat cgtaaaaatc gtacgttttc ttcctcaatg
     1861 attcgtttga tttcttcacc tgttatctgt ttctttacca tggtatccct cttccttttt
     1921 taatttattc agtcaaagaa ttgtctttgc aatccttgcg actgataatg acctgttttg
     1981 cttaacccac cttgcgccaa aatctcatca cgaaaaattt gtcgaacgtc atgatctgtc
     2041 aacggcttct tgttttttgc ttcttgtttt ttcgcctgtg ccatttcgta tacacgtttg
     2101 atcccagcca tattcaatcc ctctgaaaga tagtctttaa tctcaagcaa catatcaata
     2161 tcattcagcg aatacatacg tcggtttcct tcgcttcgtt ccggatgaac taaatcttgt
     2221 tcttcataat agcgaatttg ccgagcagac aaatcagtca gcttcataac tgtaccaatt
     2281 ggaaatacgg acatagatcg gcgcaattcc ttttctccca tcagacgtcc cctttcgttt
     2341 tataagttgg ctaaagtgta ccaattaaaa aaaatattgt caactgcata tgttaggttt
     2401 tctaacatga gatctctatt ttttataaaa taacaaaaaa ataagaacac aaaaaaggta
//
LOCUS       DAXWOO010000235         2454 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.241, whole genome shotgun sequence.
ACCESSION   DAXWOO010000235 DAXWOO010000000
VERSION     DAXWOO010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.241"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(50..1816)
                     /locus_tag="ACGE70_002769"
     CDS             complement(50..1816)
                     /locus_tag="ACGE70_002769"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8694354.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQGGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAACL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKVVDQGTHSELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1776..>2454)
                     /locus_tag="ACGE70_002770"
     CDS             complement(1776..>2454)
                     /locus_tag="ACGE70_002770"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8694355.1"
                     /translation="QKRIYALVGKSGSGKSTIAKLISGFYNVDKGQIKIGGKPLNHYS
                     KDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGCESILEKFPERE
                     HTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEYELQKAFQHLMK
                     EKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQLVTNYENANDWR
                     LDNEKFL"
BASE COUNT          776 a          474 c          350 g          854 t
ORIGIN      
        1 aattataact atatcccaaa aattgtaggt taatttaaag cctatttcat catcgtgatt
       61 gagcttcttt ttcatagacg aatttctcag atagtttagt tttctttatg agatttttgt
      121 atatttctga ccttcgatac aactcactat gagtaccttg atcaactact tttccttgat
      181 ccatcactac aattttattt accttttcaa tagacttcaa gcgatgcgat ataattagta
      241 ctgtcttatc ttgtattaaa cgatttaaac tttcctgaat acgtttttca ttttccgcat
      301 ctaaactggc agtaatttca tctaaaatta aaattggcgc atttttcaaa aaagcccgtg
      361 ctatggatag gcgttgtcgc tctcctccag aaagtgaact tccgttttct ccgaccaatg
      421 tatgataacc gtcaggtagt ttttcaataa agtcttcgca gttagctaaa caagctgctt
      481 ttttcacttc ctcatctgac gcttgactat ttccaatacg tatattttcc aaaatacttg
      541 tattaaacaa agttacctct tgaaaaacaa tagcgatttt gctaaacaaa gaagctggag
      601 atacacgttt aatatcatac ccatcaatta aaatacagcc ttcatcataa tcatacagtc
      661 tagacactaa tttcaagata ctagttttcc cggaaccact agcacctact aacgcagtca
      721 cttctccttg tttagccgta aaagagatat gatctaaaat tggggtattg ttatcatatg
      781 agaaagagac atctcgcaac tcaatgtcaa aagatttcag aggactatct gatccccctt
      841 ggatactggt ttccttcata gctctaattc gctgtatttt aggagctaaa taaaatattt
      901 caagcactgc ttctttcata gaatcgaagg aatcctttat ttttattgcg gctagtaagt
      961 aaccaacgac gtagagtata gtcacttcac ccgttaacag taagtgaaca cctactagaa
     1021 tgactactgc taaactaaca tatgagaaaa tagaggataa ggccatgact gaaaaagtac
     1081 ttaattctac ctttaaatga atcctttcac tctcttccat ttttttgaaa agtctgtctt
     1141 gaactttttt agatagatta aagctattaa tctcttgctg caattctata gtttcttgaa
     1201 attcttccga gttttctctc aaagtatcgt aatatttagt attggctttc gtttgggatt
     1261 tctttgataa caagattaag acaaaactaa ataacgttgg caaaataaca gctaatccca
     1321 ttttgacatt accaaccaaa agcatcactg aaataaaagg gaaaaacaga gccataccgc
     1381 cggctttagg tattgcatga ctcatcgcat gctcaatacc ttcaacgtca gacatgatag
     1441 tttgagataa atctgataaa ttatgttttg aaaaatagga taatggtaaa tttgacaaat
     1501 cgtctgcaat ttgcactctt aaatgcgcac tttctttata agtactatta tataagtttt
     1561 catactccct atctaacaac caatacatga aaagcaaagt aacaaacgat acggcaaaat
     1621 aaagccaacg gggctttgta ttttctaaaa ctaattcgtc tatcagcatc attataatga
     1681 atgctggtac catattaatg cagtatacaa aaaaactaga aatagaagct ttagttaaag
     1741 cttctgcgcc ctgatcactt aaagcaaatt tgtttttata aaaacttttc attgtcaagc
     1801 ctccaatcat ttgcattctc ataattagtg accaattgtt tatacacttg atgtttagcc
     1861 attaattcat tatgatttcc acgttctact attttccctt ctgacaagac aagaatttca
     1921 tctacagatt taatgctgga taatcgatga gctatcataa taactgtttt ttctttcatc
     1981 agatgttgaa aagccttttg tagttcatac tcattatctg gatcaataga tgcactggct
     2041 tcatccataa taacaatctt tgcatctttt aaaattgctc tggcaatagc tatacgttgt
     2101 ttttctcctc ctgacagata aactccttta ctaccaatta aggtatgctc tctttcggga
     2161 aatttttcta aaatcgattc gcaaccggct aatcttagcg cgtcaaatac tttacttgct
     2221 ggcgcttgag gatttgctat agaaacgtta tcgtaaatac tctgattgaa tagtttcgta
     2281 tcttgaaaga caaaagcaat tgtgttaata attgtgtctt ttgaatagtg atttaaaggc
     2341 ttaccaccaa ttttaatctg tcccttatcc acattgtaga aacctgaaat tagtttagct
     2401 atcgtactct ttccgcttcc tgattttcct actaatgcat aaattctttt ttgt
//
LOCUS       DAXWOO010000236         2435 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.242, whole genome shotgun sequence.
ACCESSION   DAXWOO010000236 DAXWOO010000000
VERSION     DAXWOO010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.242"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..209
                     /locus_tag="ACGE70_002771"
     CDS             <1..209
                     /locus_tag="ACGE70_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694356.1"
                     /translation="AATILCCGLIFSQLSIYPRYQRLFIHFLNIQCASMPLEVIKKLL
                     INPIFMVSLVLLTGLVTWVLGKKE"
     gene            252..2351
                     /locus_tag="ACGE70_002772"
     CDS             252..2351
                     /locus_tag="ACGE70_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HFF8694357.1"
                     /translation="MENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQIKEN
                     NNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAHYQKV
                     GTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQSINV
                     MNAKLEFKYAGTKTKVALSEPQPEIQSKMRSMRAAGIDLNDTFLSIYSLNGQYQQRVS
                     WYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSKVVGG
                     RAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTALLWTP
                     QPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKSFKIT
                     DTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSFLANA
                     ANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNTVENYFSLKPNKKVE
                     QDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQFSIQDPVED
                     RLTYKEGSARVIDMSTGKDITSEGTLTYDSNSRTLKWEASADFLSKNLLDGREIQLIF
                     TAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNHLVLTVIGS
                     ILFLTLASLGYVVLKLK"
BASE COUNT          876 a          376 c          473 g          710 t
ORIGIN      
        1 tagccgcaac aatattgtgt tgcggtttaa ttttttctca attatcaatt tatccaagat
       61 atcaacgatt gttcattcat tttctaaaca ttcaatgtgc ttccatgcca ttagaggtga
      121 ttaaaaagct cttgatcaat ccaattttta tggttagttt ggttcttctt acaggattgg
      181 taacgtgggt attaggaaaa aaagaatgaa aagcttacga tcgtttaaaa aaatattaga
      241 tggagggcat tatggaaaat aaaattcatt gcactacggg aagagtaatt actttagtgt
      301 tgtttttgtt ccttcttctt tttgcgaggg gaactgcagt ccaggcctta gaaaaagttg
      361 atccgagtca aatcaaagaa aataataatc tggtgcttta tcatgatagt gattcattga
      421 gaaatatcga agtaactggg atcaatggat ttaagatact agatgagtat tcaactaaaa
      481 aacaaaaagt acttgcttgg ccaaaagtca aagatttgaa aagtacgtca aaaatcgttg
      541 cccattatca aaaagtaggt acttatctag gaaaagaaat tgattgtgac gtgacttttt
      601 ctaattttgt agcgcacgct ggggttaacc aagacggatt agctaaaaca attaatggca
      661 aaaagtatta taattatttg ctctttgata aaaatattta tcgaggattt ttcatgcagt
      721 caatcaatgt catgaatgca aaactagaat ttaagtatgc aggaacaaaa acaaaagtag
      781 ctttgagtga accacagcca gaaattcaat caaaaatgag atcgatgaga gcagctggta
      841 ttgatttaaa cgatacattt ctatccattt acagtttaaa tggacagtat cagcaacgtg
      901 tgtcttggta taatgacaat aatgaatctg tcggtgaacg taatattgat atgagagaat
      961 ttgttgggta tgaaaaaatg ggtagcttac cttattttgt cacaacagat acagcatgtg
     1021 cagaatacaa agctcctgcg ttatcaacaa acaatttaac ttcaaaagta gtgggaggac
     1081 gtgcagaaaa ggcttatagc tcgaatgatc atttcaccga tgttgtagga gctgatactt
     1141 atcacagaag tggtgtaacg tatacgcttc aaggcgcttc cccaacattc atgattggcg
     1201 caaatacgaa tagtatgatg tttagctttg atactgcatt gctatggaca ccacaaccat
     1261 cgaagcctac aaaagaagtg tttaacaaag ctaatactga agaggcagca cacaatattg
     1321 acaaaaaagt gattccacaa ggatcagatg tttactatca tattcaccaa aagtttgatg
     1381 cattaacagt caacacaatg aacaaataca aatcatttaa aatcactgat acctttgaca
     1441 gcaaaaattt tgatatggta tcggatggga aaaactatga tggcgcattg tggtatgttg
     1501 aagcgaatgg tacttggaaa aaagctgata caggaggaaa gttaactgta tcaggtaata
     1561 cagtcactta tactgcttct gacagctttt tagctaacgc tgcaaattac ggtagggatt
     1621 atattttgca gctccatatg agagcaaaaa atgatgcaaa aggaattgca ccaaatgtgg
     1681 ctactgtaac gtttaacaat gatccgaaca aaggaacaca aaaaacaaat acagtagaaa
     1741 actatttctc tcttaaacca aataaaaaag tggaacaaga tgggaaagat gttgatggtc
     1801 gaaatgatgg taaaaaagga acgcctacag ctcctttaaa tgcaggatct gaagtacaat
     1861 atctagtcac acaaaaatgg catacaaaag gtgtagacat tgcctcagat cattacaagc
     1921 aatttagtat tcaagatcca gtggaagatc gtttgacgta taaagaaggt tctgcgcgag
     1981 tgatagatat gagtacagga aaagatatta cttcagaagg tacactaacc tatgatagca
     2041 attcaagaac gctgaagtgg gaagcttccg ctgatttctt aagtaaaaat cttttagatg
     2101 gacgagaaat tcagctgata tttacagcta aaactccttt gaatgaagaa ttggatatcg
     2161 acaaccaagc aaaagtttca gtagaaagta ttgagaaagc aactaatgta gtaacgattg
     2221 gggtaaaacc taacttacct cagatcattg tgccaaaaac tggatcgaat catttagtgc
     2281 taactgttat tggaagtata ttatttctta ctttagcatc attgggctat gtagttttaa
     2341 aactcaaata aatataaaaa caaggctgag aatcagcctt gttttctttt ggctctatac
     2401 tttttgatgt tggtgaactt caccaacatc ttttt
//
LOCUS       DAXWOO010000237         2434 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.243, whole genome shotgun sequence.
ACCESSION   DAXWOO010000237 DAXWOO010000000
VERSION     DAXWOO010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.243"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(6..251)
                     /locus_tag="ACGE70_002773"
     CDS             complement(6..251)
                     /locus_tag="ACGE70_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694358.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(268..1119)
                     /locus_tag="ACGE70_002774"
     CDS             complement(268..1119)
                     /locus_tag="ACGE70_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFF8694359.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            1285..2289
                     /locus_tag="ACGE70_002775"
     CDS             1285..2289
                     /locus_tag="ACGE70_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFF8694360.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT          780 a          414 c          488 g          752 t
ORIGIN      
        1 tctttctatt ttttcggtat caaggcaaaa acaggtagtt caggtgaata aatgacttca
       61 tgttcgtgcc agggtttgac cattagcgaa tgatcgaacc gttttttcag ttcttcgatg
      121 atttgatcat gtgtccagtt tcttgcagga aaatgttgga ttttctctgc atcgatcaaa
      181 aagacaaaat cggaatgaaa agtctgggta acttcttccg agagagaatt caagcgttcg
      241 attggatcca taaaatcacc tttcgactta aaattgggta gtatctgggt cttttgcttt
      301 gccgactaca ccatctaact gatcgatttg tttcatttct tcttctgtta attcaaaatc
      361 aaataattca gtattttctt tgattctgct tggtgtcact gacttaggaa gaggaaggaa
      421 ttcgtgttgc agcgaccaac gcaaagcaac ttgtgcaatc gtcttgtcat gtgcttcagc
      481 gatttgtttc atttcaggca catcgaagat tttaccagtg cctaatggac tatatgcttc
      541 gaggatcata tcatgttttt ttgcatactc aactacagtt gtctgtaatt cgcctggtgc
      601 aaggaaaact tgattgacca tcggcataat ttttgctgtt tttgctagtg tatctagatg
      661 gtggggtaag aaattgctca caccgattgc tttgatttta cctgcttcat acagttcttc
      721 aaatgctcgc catgtatctg catttgcttg ttcccaatta tcacgaaaag caacagggtt
      781 tggccagtgg atcaaaaaca gatctaaata atctgtttgg agcttcttca atgattcttc
      841 aaaagaagac atcacaagtt catagctgtg gtttgcattc cataattttg ttgtcaagaa
      901 tatctcttct ctaggaattc cactttcctt aatcgcttgg ccgacgcttt cttcattctt
      961 gtatccttgg gcagtatcga tatggcgata acctgcagcc agcgcttctt taacagagga
     1021 aacagcgaca tccccatcag gtgtttgcca tgtgccgaat cctactaccg ggatttcgta
     1081 accatttgat agacgataag tgcttgttaa tgaattcata ctttcagctc cttttttatt
     1141 cgttcaacta taagtgtagc gcatttttcg aaaaaaagaa tccattttgt ttctactctt
     1201 gaaatttttg tttaaaagaa aatgattttg attctggtat ttttgatttt ttttccgtaa
     1261 actaagacag aagggaatga gaaaatggat atctatttaa aaaaagcaat tctgcatatt
     1321 attgacagag aaactggttc tccagtcttt tcacaaaaag agctggatct gacaaaagaa
     1381 tatattcgtg attttcttca aaagaagatc caaaaaatct cttctgctca aacaaaaact
     1441 ggacaactag cagaagaaag catgatcgtt gaatcactta aaatcgctca agaaaatttt
     1501 gtggaagcaa gtgaagcaat cgttcagcgg tggttcgata tctaccaaga aagtgaagaa
     1561 gcacctagtg cagatgtatt tgttgtcttg tatgaattag atacagtgat gtacttatct
     1621 ttattgaaag tcaattatcg tgaggcttac actcactttg ttgaagcaga cgaagcaggg
     1681 atcgacaacc gcttgatttt gaatcgagca attctagggg cgaaaacaca aaaagcagat
     1741 gaagcattcg ctgtgaattt gactgattgg acctacgaat taattgaaaa aaaatatgag
     1801 ttttctggca aaaaagagct ttatttttct ggaagagtga tcgaaagtgt tcctgcacct
     1861 tctttagaag aaaatgtaaa agtgatccaa aaagtagcca agcatcttgg aaaaaaattt
     1921 gagacagaag aatttgatat catggctgat ttgaaagaag ctgtctatga tacgatagaa
     1981 gaaaaaggtc ggttggatca tgagatgatt gcggaaaaag tcttcaagga aaacatcacg
     2041 gcaaaaatgg cttttaaaga agaagtacaa gagcaaggat ttgtcccaga agcacctcct
     2101 gtaaaagaag tacaagaaat ttctgagaaa aaatttggaa aacaaaaatt caagctatca
     2161 aacgggatcg aactgatcgt accagtagat gtatatcgca acccagattt gattgaattt
     2221 gtgaataatc cggatggtac gatatccgtg atgatcaaaa atgtggatga agtattgaat
     2281 cgtttataaa aatgactaga atattaggtg ttcaagtacc agtgatcagt taaatgaatt
     2341 gtgtcaagag aaggatcgga aaagacgaat ataacaaaaa aagagcgtga gacaaaagta
     2401 aagaacactt ttgtctcacg ctctaaacac gtat
//
LOCUS       DAXWOO010000238         2403 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.244, whole genome shotgun sequence.
ACCESSION   DAXWOO010000238 DAXWOO010000000
VERSION     DAXWOO010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.244"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            26..238
                     /locus_tag="ACGE70_002776"
     CDS             26..238
                     /locus_tag="ACGE70_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694361.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPTELVS"
     gene            complement(516..2315)
                     /locus_tag="ACGE70_002777"
     CDS             complement(516..2315)
                     /locus_tag="ACGE70_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFF8694362.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRSFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT          840 a          440 c          450 g          673 t
ORIGIN      
        1 aaaaataaag aaaagaggga atgaaatggt aaaagcagaa gtaggaagat cttatatatg
       61 caaaccgatc ggagtatctc atgaactaat cggctgtatt gaacgtacat atatcaacac
      121 ggctgtcgtg tatgttgaaa gttatgacca acgagactat caacttatca atgaaagcaa
      181 gtatcgcatg ctcgtaaaat tctgtgatat tagcgcacct actgaattgg tttcttaaac
      241 agtatttttg agaattcgca agaactccct tataatataa ataaagacag aagggagcga
      301 gaagatatgc caaaaagaaa aagttcaggg aaaatgggaa ttattcttgc agtgatctct
      361 gcaattcctg ctattttagc tattgttcgt gcgatgaagg aatcaagaga aagttaagca
      421 cacattatga gcgtcaaaca aaaaacgcat atacctgcgg tggcaggtta tgcgtttttt
      481 gttgtataaa gacttttttc attaacgatt tttgattagc cgactcccaa aacaaaattt
      541 agcagtttat ctgaataagt gatttcttca tctgtttgtt tcacactttc atttagtagt
      601 cccatctcat cggttatgat accatccaca ccataaaaca tcatgcgact catggcatct
      661 gaatcgttga ttgtccatac atatacttct tttccgtctt ggtgggcaga attaatgaag
      721 ttacggttga tcgtactata ttccattgtc agaaaatcag caggcgtcac agggggccca
      781 acaatattga aaggtaagat gtagcccaca taaaagtttg gttctgcttc ttttaaatct
      841 gaaacggttt ggtacgtcaa actttgcaaa atatgatggt tctctaagat aactttgcgg
      901 tatttatgga caaatcgttc gacaaggtca tccgaatctt ttttagaggt cttgatctca
      961 ataagtaatt tttggtttag cttatgtgct ttttccaaat aagaatcaaa agaacaaaca
     1021 ggggcttgca ctccgttttc agtaactgta agatttgtca attctttcaa tgtaagatcc
     1081 tgtggtgttc catttacccc tgttaagtga cgaagatttg catcatgcat cactacgaaa
     1141 tgaccatctt ttgtttcctg aacatccatc tctacataat caggatgata gtctctgctt
     1201 gttttttcaa gtgcttctaa agtattttgc gccccatttt gggtactcac acctctgtga
     1261 gagacagtca ctggttttgt ctgaacagct gaagtcagat aatccatatt gtaaagacag
     1321 acgccaatac cgaaaaaaac tgcaaaaaga gtcaaaccag tgtttttcag cgcagagaaa
     1381 cgcaagttgg gggcttgtgg gataaaccac ttagggatct caggcaagaa gccttcgtca
     1441 tccatgaaat caacaatgat ataaaaaatc cctacggtag acatcacgat attgaataat
     1501 aaaatccctt gtaaaagcgt catagcaaac acagcgctga tcaatgcata gtctgggaaa
     1561 aattgttcaa ccatactttg tgccaaaata acaacgatat atcctaaaga agaaaggagt
     1621 aaaatcgtac cgccaatcac aataaattgg cccgtgatgg ccaaaagacg cgatttggtc
     1681 aatgaccagc tttcccgaat ggctgctcga aaagaacgat ctcgtagaat catttccggt
     1741 aaagcaaaaa tcagtcgaat cccaatatag ccaaggaaaa tataaacgag taaaaaggaa
     1801 ctgacgatga tccaacgatt agcaaaaata aaatccataa tgaaagcggg gatttttatt
     1861 tttgacagta gatcagaatt aaaacttaat ccgctgatcg gtagaatcaa taaaaaataa
     1921 gcaaggaaaa acaaaaaagt aatgggtctc actttcttca gctgaagtat cgtcaagtgt
     1981 aagagttgct tcaaagagat tggttctttc tttttaataa aaaaaacact catcaaaaga
     2041 aaagtaaatt cgaaataaac taacagtaaa atcgtcatca aaactacgat taacgagagt
     2101 agaaccccag gatgttgaga aaaaattgaa ggaatattgt cataagacaa atagtcaatt
     2161 gcccctcggt gtaaaataaa acgggtcgta ctgctcaata agggtaaaca aataaataaa
     2221 ataaagccat gcatcagcaa gacatcacgg aaataagctt gtgttccttt taaaaagtcc
     2281 caagcattac ctaagctacg tttcaaataa ttcatcctat cacctgctct tcacattgta
     2341 gcgcaaatct aatgaaaaag gaaagggtgc agtgtgtttt gcagaccctt tcccagattt
     2401 aac
//
LOCUS       DAXWOO010000239         2389 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.245, whole genome shotgun sequence.
ACCESSION   DAXWOO010000239 DAXWOO010000000
VERSION     DAXWOO010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.245"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            196..2058
                     /locus_tag="ACGE70_002778"
     CDS             196..2058
                     /locus_tag="ACGE70_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFF8694363.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2058..>2389
                     /locus_tag="ACGE70_002779"
     CDS             2058..>2389
                     /locus_tag="ACGE70_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFF8694364.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          859 a          353 c          465 g          712 t
ORIGIN      
        1 aaagcgtttg attatttata ttacgactta gtgcaataaa aaacgctttt taacagtaca
       61 tgtagtcgtc tttataatag ataaactctg taatattagt taaaaagcta taagttgcac
      121 ttagttaagt gcaaacattt catattgtcg ttagtttaga aaacgcttat attagcaatg
      181 taaggagaga tgataatgca gcagctaaca aaaagagaaa ggcagctttt gatttacttg
      241 gtggaaatgt ctgaaccggt aacaatcaaa aagttggctg aaaaagaaaa tgttagtgtt
      301 cggacgataa aatacgatct agatgatata agagagtggc tgaatgagcg gaagcaagtc
      361 ctatatagca aacgtagtca gggaatttgg atgaatatta gtagctctga tcgaatcaaa
      421 ttaaaaagcg aattgatgga tgttaatcgt tttgaaatat ttgcagatca aagtctccga
      481 atcaaacgtt tactaattat gcttttagta acaaaagaag ctattactgg aatgaaaatg
      541 gccaacgcat tagatgtcag caaagatacg atcatgaatg atttagatgt gctggaaaat
      601 aattttctta aggaaggatt gaccttgaat cgtcaggcgc gaaagggttt ttggatcata
      661 ggagaagaac gtctagttcg attaacgatt gaagagattc ttcaaaaaga gttcacggat
      721 tatgacattt ataaattaat gtcattgtta ttgaatggcg gtgagactga atactttgaa
      781 atgtattctg ctactgctac acctatccaa gaggtattta atcaagtaat cattcgaatg
      841 cgacacttac tggaatttga aaatctagag aaactgaatt acgcggaatt actaaatatt
      901 ctgattcgag tgacaattgc taccgttcgc ttacgcaaag aggctacgat tggtcgttat
      961 caattggttg ctgaacaaga attagacaaa aacgatcttt cttatcgcgt catgcagcag
     1021 gtctttgatt attatcagtt gccactgttc gaggatgaat atcattatat ctatagtgat
     1081 acattcgaca cgattcacca gcaagacgtc atgaaactag ttgagaatgt gatttataaa
     1141 gtaagtaaag caatcaacca ttctttttat caagatccac aattgttgac gaatctgtat
     1201 gctcatttat ccatgcgatt atcgagaaag aaaaaatttg tcaatgaata taatcctttt
     1261 aaagaagata tcaagcagaa atacccggaa ttatttgatg cgatcaagca agcagttcga
     1321 caagaaataa gtgaacaaaa tattctgatc aatgattctt tcgtggcata tattgcctta
     1381 cactttttgg tttcctacga aagagaagaa gatctacgaa gcgttagagc cgtctacatt
     1441 tgttcaacag ggttgggtgt gactagtctg atcaagcaaa agatcaatga agaattaaat
     1501 aatatcacta tcgtggcatt tgcttcagta ttgaatgcgc aagaaatgat tgctaccaaa
     1561 gatcctgatt tggtgattag tatttttcca attaatggca tcgatcgtcc tttcatcaaa
     1621 gtccatccat tacccactga acaggacttg aaaaaaattc aacaaataat aaaaaaaata
     1681 cttgcttctt ccaatcagca ggaaaggcat aacttgaatc ttagagaaac gtccatcaat
     1741 aaagatactt tagaagactt tagcaaggaa ttgattttga aagcttatac gatttatgaa
     1801 cgtttacttg tcattttgga agcagaattg atccaagaat atcgagaagc ttttttgctt
     1861 catgtattct taatggttca tcgtatcaca tttgatcaac aatatatggc agaaggtcgt
     1921 caagtagacc agattatttt agacaaagga acactggtag gaaaaatcga agcagttttt
     1981 gcagaaaata atttaggaat caatcagtca gaaatcagtg cattattctc atatataaaa
     2041 gaaggagcgt caagttagtg aaaatgtcag acgaggcttt aaaaattatc gaggaaagtc
     2101 aaaatcggga aaagttaaaa caagtgattg atctaattga agagcgatta gtagaacatg
     2161 ctattgtacc aaccgagttg cagtggacca ttctaattaa tcacttgaat gagatgctga
     2221 aacgttctca aagaaaggaa acgattcccg tagtcgatcg ggatttgttc aaagaagtat
     2281 ccaaagaagc cataactatt tctgatgaaa tagttagaag tatcggtaat ttaaccgatg
     2341 atgaaatata tgttttgtcg attcattttg aaacagcaaa aaataatta
//
LOCUS       DAXWOO010000240         2387 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.246, whole genome shotgun sequence.
ACCESSION   DAXWOO010000240 DAXWOO010000000
VERSION     DAXWOO010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.246"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(102..709)
                     /locus_tag="ACGE70_002780"
                     /pseudo
     CDS             complement(102..709)
                     /locus_tag="ACGE70_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            1232..1906
                     /locus_tag="ACGE70_002781"
                     /pseudo
     CDS             1232..1906
                     /locus_tag="ACGE70_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
     gene            1899..2060
                     /locus_tag="ACGE70_002782"
     CDS             1899..2060
                     /locus_tag="ACGE70_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694365.1"
                     /translation="MTEKEQVQQIVKKYNKSIADLSENASAKEFKTVMNYVADEANRK
                     QRKLVGLDK"
BASE COUNT          851 a          366 c          396 g          774 t
ORIGIN      
        1 ctatgaatat aaatagagtg tatcttcttt gataaatgtc agaaaaagtg tgacaaataa
       61 ccatcagtaa aattacttgt catactattg ataaggagta tttaattgaa gagaatgatt
      121 aggagaaagg atctccgtac tttttatagt aatcatagaa tccaagtttg tatagacgaa
      181 tctttttttc tcttatttct tctgtttcaa aatggactgc ttgaagaatc aagtctgtga
      241 attctatagg agctgttcca ttcgcagtaa tcaaattacg atctttcaca gcttgttgtt
      301 ctataaaatc ttctttattc tgataatttt ccgattctaa ccaaggaaat tgcgcatttc
      361 cggtgtgttt gtaaccttgc aataatccgc tctgagctaa ataatcaact gccccacaaa
      421 tagcagctac tggtacgtct gtttttaaca atttatcgat tatttgatat aaatgatgat
      481 tttttatgtc ccatgaattg ccaccgatca taataaaaag gtcaactttt tcaggtattt
      541 gttcgattaa gtaatcgatt tttgttttta atcctccaat ggaagtgacc tctgattgga
      601 tagaagcagt tttgttttgc catccctctt tttggtttag tgtgctagta atataagcac
      661 cttcccaatc ggcatattcc tctaatagaa acgtaatcgc agttttcatt ttcaagctct
      721 cctttactat ctatgacaag gacaacagtt ttaacataca gacattcatt aaatttggat
      781 agcgacagcg aaaacaacaa ttcttaagtt ttagacagag tatacagaca gtcaacttaa
      841 ttaaatacga actaaataaa aatgacaggt aatcattttt gtttaccagc attataacat
      901 tttgcataca tggatgacaa aagaaccaat tcccacttgc ttgagtacaa tcttaaaaga
      961 aactggaagg caaaaagtaa ttatagaaac gaaaaataaa tcatatcggg cactcagaca
     1021 tgaaaacaac cctagaaata tataactagt ccaatgaaaa aaaagcgaat gccgtagaaa
     1081 acaaggattc tgcgtacatt catatctcat tttgcgagca tatctgctat tactgtgact
     1141 aaatgttaat atggttaaaa tgaccgttat attttttata taaatatcgt ataattagta
     1201 tgaatataga agaaggaaag aaggtgcaat cttggaacct aaagacaagt atttagatta
     1261 cgctagggtc aataaagata aatttattga aacaatcatc aagggaaaaa tccctgatga
     1321 tgaaaaagat gcggtattca tggctggaag cccaggagca ggtaaaacag aggttgcact
     1381 aggtttagct taaaattatg acaaccatgt tgtgattgat gcggactatt ttagagcaca
     1441 atttccagaa tacaatggaa agaactcaag tgtttttcag aaagcttctt cttggttagt
     1501 tgaacaatca ttaaaatatg tactggaaca tggctattct ttcatactag aggatacttt
     1561 tgctatcctt agtgcagaga aaaatattat tcgagccctt aaaaataact ttcgcgttac
     1621 gattttctat gtttaccaag atcccaaagt tgcctgggat ttcactcgaa aacgtgaact
     1681 agctgaagga cgtcatgtgc caaaagaaat gtttatcaat gcgtttttta agtccagaga
     1741 gaatattgaa aaagtcaaaa atagacatcc tgaagtagtc cttcacataa tgattaagga
     1801 ttatcaaaac gacatttctg aagtgcatta tgcagcagat aatattcaat tagtactacc
     1861 aatacaatat acttcaaaag agttggagga ggaattacat gactgagaaa gaacaagtac
     1921 aacaaatcgt gaagaagtat aataaaagta tcgctgattt atcagaaaac gcctcagcga
     1981 aagaatttaa aacagtaatg aattacgtag cagatgaagc aaatcgtaag cagcgaaaac
     2041 tagttgggtt ggataaataa attttttaaa ccatatcctt gtaaagttga gttttttatt
     2101 ttaatgaatt aagactatta aatagataat tattgtaact taaaaaacac agctaaaaat
     2161 tagttgtgtt ttttattagg tttacttttc tggtataaaa ggaactagct actttttcaa
     2221 aatttctaaa tcattcaata tgtagcgctt actaaattat tcgccataaa attttataat
     2281 tggatcactc attgttttac ctaagtcata tttaagtaat aaccggtgtt tttacataag
     2341 aagacttcgg gtatttttta gtttgattag ctctgatata cctataa
//
LOCUS       DAXWOO010000241         2373 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.247, whole genome shotgun sequence.
ACCESSION   DAXWOO010000241 DAXWOO010000000
VERSION     DAXWOO010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.247"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..359
                     /locus_tag="ACGE70_002783"
     CDS             <1..359
                     /locus_tag="ACGE70_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694366.1"
                     /translation="QNNKNNSGCGIIFMFVIVLGIVVLLITVKIVDYFANTPEEQIQR
                     KLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKKKEYNYIVKAKVSK
                     RFIFNPKLEIYSVVIE"
     gene            684..1301
                     /locus_tag="ACGE70_002784"
     CDS             684..1301
                     /locus_tag="ACGE70_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFF8694367.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            1342..1641
                     /locus_tag="ACGE70_002785"
     CDS             1342..1641
                     /locus_tag="ACGE70_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HFF8694368.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1675..1842
                     /locus_tag="ACGE70_002786"
     CDS             1675..1842
                     /locus_tag="ACGE70_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFF8694369.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(1887..>2373)
                     /locus_tag="ACGE70_002787"
     CDS             complement(1887..>2373)
                     /locus_tag="ACGE70_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFF8694370.1"
                     /translation="NSLRYLEFREGAFDFQTSQQNPSLDRLGRDYEDIKNTVAFMKQK
                     KVALKILDAQFLDFNTGNELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKA
                     GRYKGRPIEYSINSTDPQKRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKAND
                     L"
BASE COUNT          823 a          363 c          452 g          735 t
ORIGIN      
        1 ctcaaaataa taaaaataat tctggatgtg gaattatctt tatgtttgtt atagttttgg
       61 gaatagtagt acttttaata actgtcaaga tagtagatta ctttgcgaat actcctgaag
      121 aacaaataca gagaaaactt cctgagtttg aaagagtacc accttatgat ccaagagata
      181 attcatttca agtaaaaaaa ttaaaagagt ctccaaatga aatgtggttt gaagttacag
      241 ttgaatcaag cgacttcaaa gaaaaaaaga aagaatacaa ttatattgta aaggcaaaag
      301 tatcaaaaag atttattttt aaccctaaat tagaaatata tagtgttgta atagaataat
      361 agtgttaaaa aaattgtaaa agttaaagaa gtcaaattct gatgtgaaaa ataaaatata
      421 ccatggaaga cataaaagaa aaactggata actagtttat ttaatggcag accgttaagg
      481 cctgccattt ttatgtattt tagcggaata aatataatca tgcgggagtg taaacttact
      541 gtttcaaaaa gtatactttt tgacatgcct tgattttctt tgtttatgta tggcataata
      601 accacataat acaatttgaa acgtttctga tttaatctga ttgttttgaa acaattttag
      661 tgaaaaaagg gtagataaaa acgatgagaa aattttttta tatccgggtc agtagttatg
      721 atcaatcgat cgaacgacaa ctgatttccg cgaaggaatt aggaatccct gaagaatata
      781 tatttgtaga aaaagccagc ggcaaagatt ttaaacgccc tgaatatcaa ttaatgaaac
      841 ggatgtttcg aaaaggagat ttactctatc tacaatcatt agatagacta ggcagaaata
      901 aacaaatgat tttagaagaa tggcgagaat taactaagga aaagcagatt gatatttctg
      961 tgttagacat gcctctgtta gatactgcta gttacaagca actagatggg ttagagacgc
     1021 ttatctctga cttaattctg caattactca gctatatggc cgaagacgag cggaaacgaa
     1081 ttaatagccg gcaaagagaa ggcgtagaag ctgcgaaaca aaagggtgta aaattcggca
     1141 gaaagaaaat tgctttacct cctgaatttc ctgcgatcta ttctgactgg aaagcaggaa
     1201 agataactgc agtctatgct atggatcaat tgggcatgaa acgaaatacc ttttatcgac
     1261 gggtgaaaga atatgaacat accttgcacc acttttcttg atagatataa cagctattag
     1321 acgggaaggg agagtttgaa aatggactta acgaatcaaa gggtcgtttt tactggaacg
     1381 ctgcagcaaa tgacacgaac ccaagccatc ggtttggttc actctttaaa cggccattgt
     1441 caatcaagtg ttacttcaaa aacaaatttc ctggtttgtg gtcagtattc taagtcgttg
     1501 tttgatgatt ctctctatac aaaaaaggaa caaaccgcgc gggacttgat tgcgttaggt
     1561 catgagatta ccattttatc agaagtcgaa ttttatgcgt taattagtca acagttgaaa
     1621 gaatggcaac ggtatttata atcgttagta aaatttacaa ggaggaaaat ttttatgaag
     1681 cgaaaatctt atgatcgttg gggcattgaa ataactagtt ttaatcgaaa tattgctttt
     1741 cacgtggagt gtgactgtgg ggaattagca aaaagtgttt atcaaaaaaa ttacttttac
     1801 tgtcctaaat gccatcgccg atacggggta catggaacat agaaacgaaa tagtgcggta
     1861 gaaaagcgaa tgaatgtagt atatttctac aaatcattcg ctttctttat tttataaata
     1921 gttggtcgag agatcccgtt ttcttttgca atctctgcaa ctggaatgcc ttgctttaac
     1981 atgtccacca cggttttaaa aactaagcgt ttttgcggat ctgttgagtt aatagaatac
     2041 tcgattggtc gtcccttata tcggccggct ttttgagcca ataatactcc ttgccgctga
     2101 cgttcacgaa ttttctcacg ctcgttttgt gcaatataac ttagcagtga taaaaacata
     2161 tcgaacatag cggtatccag gagttcattt ccagtattaa aatctaaaaa ctgtgcatct
     2221 aatattttta acgctacttt tttctgtttc ataaaagcaa ctgtattttt tatatcttca
     2281 taatctcttc ctaaacgatc tagtgatggg ttctgttgcg aagtttgaaa atcaaacgcg
     2341 ccctctctga actccaaata tcttaggctg tta
//
LOCUS       DAXWOO010000242         2368 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.248, whole genome shotgun sequence.
ACCESSION   DAXWOO010000242 DAXWOO010000000
VERSION     DAXWOO010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.248"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(<1..132)
                     /locus_tag="ACGE70_002788"
     CDS             complement(<1..132)
                     /locus_tag="ACGE70_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFF8694371.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
     gene            542..2368
                     /locus_tag="ACGE70_002789"
                     /pseudo
     CDS             542..2368
                     /locus_tag="ACGE70_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          936 a          392 c          496 g          544 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaaatgata ttttacgagg aggaaacggt
      541 catggcagaa gaaaaagaac cactagacaa tagtcgtttg ggaaaatcca aaagaaaatt
      601 agtacgattg caaaatgaat taaacgaaca aattgaaaag atgtttgaac accagagaaa
      661 gaccaacggt caaccaatga acgataagag aaatgggcat tcttggttta ggcaacaaga
      721 gcgaatcgaa aataaagtgc atagtctgag agaagagatc aagcaacaag aaaaacaagt
      781 agaaaaactt gaaagacaag aagagataaa ggaaatgggc tataacaagt acggcggact
      841 ggatatgacg atcgagaata ttccaagaat caaagaagaa atcgaacgct ttgaaaaagg
      901 tgaatcgact ttttcagccg ctacgatccg aaaataccaa cggaaattgg aaacattaga
      961 acagctgaaa gaaagatcgg aaaaaggaaa agaaaacctt cttcctgaag tacaagcaat
     1021 cattgacagc ggacgagtga cgcaatggaa aaaaatccga ccatttattt tctgaaaggc
     1081 tatcgaaaag tcgctttaga actgaatgaa aacggggaat ttgaggaatc taaaaaatac
     1141 caagccacgt cggaagagga aaaagcagct gttcgtgaat tacttgaaga ggctgtacca
     1201 actgattttt tgaaggagaa agaaaacgat catttttatc gagtagaatt caacgaaaat
     1261 gatccagaaa aaggattgaa aagttatggt ggggagatcg taacattcga attgatcgat
     1321 caactcaaaa cgttggataa ccaatacgcc gcagaagcag gttattataa attctatttt
     1381 gaagaagtga agaacggaga agtagtcgat cagatccgaa tggatctagg ggatggcgtc
     1441 aacagtaatc aacccatcta tttagagtta gcaaaattgt gcgaacaagt acctgagaag
     1501 gaacaaaaac aatcggtcaa aaatgaacct gtttcgaaag aacaaatgca gtcgaaagaa
     1561 acaaaaacaa aacaaaaggg ctatcaaaaa cgaacggcag aagagattaa acaagaagtt
     1621 aaatcccttt cgcaacaggc actggatgcg gtgaaaaagc atactcattc tcctgaagac
     1681 gtcaaagaac ttttgaactt tatgtctcgt tttccagaac gatcctttag aaatcaatta
     1741 ttgattgagg aacaatttcc aggtgcaacg gcttgtttag gtcgtgccgc gttgaaaaaa
     1801 gaaggaatct atatcaaaaa aggggaacaa gggaacaaga tctttgtcag aaaaacggtc
     1861 aaaggatttt atgagaaagg acgtggcttt gttcgagaaa tagaagctac accagaagat
     1921 aaaaaacgaa tcgaaagtgg tcaaatcgaa gtgatcaaaa aaacttacta cacgattgaa
     1981 aaagtgtttg atatcacaca aacacagtta aaacctgaag actatccgaa aatctttccg
     2041 aaccgtgtct ttgattttca attggataaa aaaggacagt cagaacttca agctgggatt
     2101 caagcggtag ccaacaatat tggaatacaa attcgagata tgacagaaag tgaagtctat
     2161 caaagagaat taggacaagc acgtggggcg tatgttcgta acgaatttac tggcgaagaa
     2221 gaaatcgtca tgaatactcg aaatactcct acgcagcatt tagctacaag tattcatgaa
     2281 ttagcccatg caagaatgca caaattttca gaattagata ccgcaactaa agaattgcag
     2341 gcagaaatga cttcttatat tgtgtgca
//
LOCUS       DAXWOO010000243         2340 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.249, whole genome shotgun sequence.
ACCESSION   DAXWOO010000243 DAXWOO010000000
VERSION     DAXWOO010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.249"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            66..584
                     /locus_tag="ACGE70_002790"
     CDS             66..584
                     /locus_tag="ACGE70_002790"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFF8694372.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            712..1395
                     /locus_tag="ACGE70_002791"
     CDS             712..1395
                     /locus_tag="ACGE70_002791"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFF8694373.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1704..1777
                     /locus_tag="ACGE70_002792"
     tRNA            1704..1777
                     /locus_tag="ACGE70_002792"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1738..1740,aa:Arg,seq:tct)
     gene            1936..2295
                     /locus_tag="ACGE70_002793"
     CDS             1936..2295
                     /locus_tag="ACGE70_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFF8694374.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
BASE COUNT          793 a          354 c          468 g          725 t
ORIGIN      
        1 tttgaaaaaa atttatcgtt attatatgat taagaaggat tgaataaaga aagaagttga
       61 aaacgatacc tgattacaaa aaaataactt ttataagttc tataacgttg attatatttt
      121 taattcttat tcttctcgtc gtcttggttc cccgaatttt tggtttgact ccttatatta
      181 ttgaggacaa agaaatgagt ccatattatg cacaaggaaa tctgatatat gtacgaccga
      241 aaaatacaaa tgagatttta gtcggagata ttattacata ttatgaaaat tcaggaaaaa
      301 aaatctcgac gcgtcgcgta atagccgttg aaaacaacca cgaagatttt tatacaaaag
      361 ctgatacaaa gacttatatt gagcctggag tagtaaaaaa gagaaatatt atcggtagtc
      421 ccatctttca gattccatat attggactag tcctttcaaa aacggcattt gcatggattc
      481 aggggttatt ctttacgatt gctttttgcc taacaggaat cacagcatgg aatacctttg
      541 tagagttgaa aaagaagcgg agacgaacac agaaattttt gtaagaatgt taaaaacata
      601 aatgtattcc agagatccaa cgactcttct gaacatgaaa cacgagaaaa cactcatgca
      661 gcttgtagat ttagtcagtt gttcgtataa tgtgtgaaga ggaggagtag tatgcgcaat
      721 gaaatgatgc atcgtcctgt agtcaaagaa gaactattgg attttatgcg tacccagcaa
      781 aaaaaattac cgggtgagct aggcaaactg gaagaagaag ctcatgtagc agaagtacca
      841 gttattcccc atgaaacagt tgtatttttg aaatttttac ttggacagct tcagcctgaa
      901 cgaatcttgg agatcggagc agctatcggt ttttcatcaa gtgtcatggc cacgacaatc
      961 ggagaaaacg ctcatgttac gacaattgat cgtttcgatg tcatgatcga gaaagctaaa
     1021 aaaaactatg aacgtctagg actgacagat aaagtgactt tgcttgaagg tcaagcagca
     1081 gatatcttgc cggagttgtc tggtccgtat gactttattt ttatggatag tgcaaaatca
     1141 aaatatattg aatttttacc tgaatgctta cgtttgttaa gaaaaggtgg cgttttgatg
     1201 gtagatgaca tcttccaagg aggaacgatc ttgctcccag atgaagagat tccccgtggc
     1261 aaacgagcta tccatcgtaa attaaatgaa tttcttcgtg ttgtgatgga tcatcccgat
     1321 ttgacatcga caatcttgcc attaggtgat ggtgtgatct taatgacaaa ggaatcagaa
     1381 acaatcgagt tgtgatacat gattatggca gcctgtagat aatcgattga atcgagggct
     1441 caattcgaac ttgatgtaaa agtttctata tttgtaagtc aattaaagga tacaggaaat
     1501 ctaaataaac aggagtagca tgaagtcaat gtagctcctg ttttcaattg ataaccttag
     1561 aacagctgaa ggagaatgtc tcgttttaaa agattgattt agtagttggg ccaaaataat
     1621 tcattatttg ctaattaaat aactatttcg tattgacaga agcaatttaa ttatctataa
     1681 ttattgatgt tccgctgatt cttgtcatag tagctcagct ggatagagca tccgccttct
     1741 aagcggacgg tcgggggttc gaatccctcc tgtgacgcta aaaaaccgtt agaaactgag
     1801 gtttctaacg gtttttcttt gttttttgtg ccaatattgt ttgttttcaa aggcaaataa
     1861 aataaaaaaa catccttatc gagtacaatt ataaggagca aaaaaataaa actcatcaga
     1921 aaagaggaag aagatatgac gatactatct agaattttag tgactctagt tgcaattgaa
     1981 tttttttata tcatgtatat cgaaacggtg agaactgatt ctgacacgac aagtagggtt
     2041 tttaaaatga gtaaagaaga gttaagcaga aaatctgttc aaacattgtt taagaaccaa
     2101 ggagtgtaca atggactatt aggagtggga ctactttatg gagcttatct ttcttccgca
     2161 tctaaagaaa tcacaagtat gctattaata tctatttttt ttgttgcatt atatggcagt
     2221 ttgactagcg atcgttggat tttactcaaa caaggtgggc tagctctgtt ggcattgctt
     2281 tctattttct tataaatata gaaaaaataa gaatttatct aacaggaaac tttgtgaaaa
//
LOCUS       DAXWOO010000244         2335 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.250, whole genome shotgun sequence.
ACCESSION   DAXWOO010000244 DAXWOO010000000
VERSION     DAXWOO010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.250"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(99..170)
                     /locus_tag="ACGE70_002794"
     tRNA            complement(99..170)
                     /locus_tag="ACGE70_002794"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(135..137),aa:Glu,seq:ttc)
     gene            complement(182..255)
                     /locus_tag="ACGE70_002795"
     tRNA            complement(182..255)
                     /locus_tag="ACGE70_002795"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(219..221),aa:Ile,seq:gat)
     gene            complement(268..338)
                     /locus_tag="ACGE70_002796"
     tRNA            complement(268..338)
                     /locus_tag="ACGE70_002796"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(304..306),aa:Gly,seq:tcc)
     gene            complement(343..415)
                     /locus_tag="ACGE70_002797"
     tRNA            complement(343..415)
                     /locus_tag="ACGE70_002797"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(380..382),aa:Phe,seq:gaa)
     gene            complement(419..491)
                     /locus_tag="ACGE70_002798"
     tRNA            complement(419..491)
                     /locus_tag="ACGE70_002798"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(456..458),aa:Asp,seq:gtc)
     gene            complement(510..583)
                     /locus_tag="ACGE70_002799"
     tRNA            complement(510..583)
                     /locus_tag="ACGE70_002799"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(547..549),aa:Met,seq:cat)
     gene            complement(596..685)
                     /locus_tag="ACGE70_002800"
     tRNA            complement(596..685)
                     /locus_tag="ACGE70_002800"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(647..649),aa:Ser,seq:tga)
     gene            complement(708..779)
                     /locus_tag="ACGE70_002801"
     tRNA            complement(708..779)
                     /locus_tag="ACGE70_002801"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(744..746),aa:Glu,seq:ttc)
     gene            complement(801..888)
                     /locus_tag="ACGE70_002802"
     tRNA            complement(801..888)
                     /locus_tag="ACGE70_002802"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(852..854),aa:Ser,seq:gct)
     gene            complement(913..986)
                     /locus_tag="ACGE70_002803"
     tRNA            complement(913..986)
                     /locus_tag="ACGE70_002803"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(950..952),aa:Ile,seq:gat)
     gene            complement(1000..1070)
                     /locus_tag="ACGE70_002804"
     tRNA            complement(1000..1070)
                     /locus_tag="ACGE70_002804"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1036..1038),aa:Gly,seq:tcc)
     gene            complement(1072..1147)
                     /locus_tag="ACGE70_002805"
     tRNA            complement(1072..1147)
                     /locus_tag="ACGE70_002805"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1112..1114),aa:Asp,seq:gtc)
     gene            complement(1166..1239)
                     /locus_tag="ACGE70_002806"
     tRNA            complement(1166..1239)
                     /locus_tag="ACGE70_002806"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1203..1205),aa:Met,seq:cat)
     gene            complement(1252..1341)
                     /locus_tag="ACGE70_002807"
     tRNA            complement(1252..1341)
                     /locus_tag="ACGE70_002807"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1303..1305),aa:Ser,seq:tga)
     gene            complement(1365..1438)
                     /locus_tag="ACGE70_002808"
     tRNA            complement(1365..1438)
                     /locus_tag="ACGE70_002808"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1402..1404),aa:Ile,seq:cat)
     gene            complement(1452..1525)
                     /locus_tag="ACGE70_002809"
     tRNA            complement(1452..1525)
                     /locus_tag="ACGE70_002809"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1489..1491),aa:Met,seq:cat)
     gene            complement(1553..1625)
                     /locus_tag="ACGE70_002810"
     tRNA            complement(1553..1625)
                     /locus_tag="ACGE70_002810"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1590..1592),aa:Ala,seq:tgc)
     gene            complement(1645..1718)
                     /locus_tag="ACGE70_002811"
     tRNA            complement(1645..1718)
                     /locus_tag="ACGE70_002811"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1682..1684),aa:Pro,seq:tgg)
     gene            complement(1728..1801)
                     /locus_tag="ACGE70_002812"
     tRNA            complement(1728..1801)
                     /locus_tag="ACGE70_002812"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1765..1767),aa:Arg,seq:acg)
     gene            complement(1812..1897)
                     /locus_tag="ACGE70_002813"
     tRNA            complement(1812..1897)
                     /locus_tag="ACGE70_002813"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1861..1863),aa:Leu,seq:taa)
     gene            complement(1914..1985)
                     /locus_tag="ACGE70_002814"
     tRNA            complement(1914..1985)
                     /locus_tag="ACGE70_002814"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1951..1953),aa:Gly,seq:gcc)
     gene            complement(1997..2069)
                     /locus_tag="ACGE70_002815"
     tRNA            complement(1997..2069)
                     /locus_tag="ACGE70_002815"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2034..2036),aa:Thr,seq:tgt)
     gene            complement(2087..2168)
                     /locus_tag="ACGE70_002816"
     tRNA            complement(2087..2168)
                     /locus_tag="ACGE70_002816"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2132..2134),aa:Leu,seq:tag)
     gene            complement(2176..2248)
                     /locus_tag="ACGE70_002817"
     tRNA            complement(2176..2248)
                     /locus_tag="ACGE70_002817"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2213..2215),aa:Lys,seq:ttt)
     gene            complement(2252..2324)
                     /locus_tag="ACGE70_002818"
     tRNA            complement(2252..2324)
                     /locus_tag="ACGE70_002818"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2289..2291),aa:Val,seq:tac)
BASE COUNT          640 a          642 c          544 g          509 t
ORIGIN      
        1 aaaagcttag cacaatcatc cataaagata actctgctaa gctttttaaa atcattcttt
       61 cagataaaaa gaaaaagata ctcaaaaagt atcttccatg acccgtacgg gattcgaacc
      121 cgtgttaccg ccgtgaaagg gcggtgtctt aaccgcttga ccaacgggcc aataaaataa
      181 atgggcctaa atggactcga accatcgacc tcacgcttat caggcgtgcg ctctaaccag
      241 ctgagctata ggcccataaa aactaaaagc gggtgacgag aatcgaactc gcgacaacag
      301 cttggaaggc tgtggtttta ccactaaact acacccgcat aatggcgcgg gacagaatcg
      361 aactgccgac acatggagct tcaatccatt gctctaccaa ctgagctacc gagccaaacg
      421 gttccgacgg gaatcgaacc cgcgatctcc tgcgtgacag gcaggcatgt taacccctac
      481 accacggaac caatgttttt agtttttaat tgcgggagca ggatttgaac ctacgacctt
      541 cgggttatga gcccgacgag ctaccagact gctccatccc gcgataatat gaaaaaggag
      601 gataagggat tcgaaccctt gcacggtttt acccgcctga cggttttcaa gaccgttccc
      661 ttcagccgga cttgggtaat cctccatgta aaactatatt aaattaatga cccgtacggg
      721 attcgaaccc gtgttaccgc cgtgaaaggg cggtgtctta accgcttgac caacgggcca
      781 ggtaaaacaa aatattgaaa cggagaagga gggatttgaa ccctcgcgcc ggtttcccga
      841 cctacaccct tagcaggggc gcctcttcag ccacttgagt acttccccaa aaccaataag
      901 attttgtact aatgggccta aatggactcg aaccatcgac ctcacgctta tcaggcgtgc
      961 gctctaacca gctgagctat aggcccataa aaaactaaaa gcgggtgacg agaatcgaac
     1021 tcgcgacaac agcttggaag gctgtggttt taccactaaa ctacacccgc atggcggttc
     1081 cgacgggaat cgaacccgcg atctcctgcg tgacaggcag gcatgttaac ccctacacca
     1141 cggaaccagt gtttttagtt tttaattgcg ggagcaggat ttgaacctac gaccttcggg
     1201 ttatgagccc gacgagctac cagactgctc catcccgcga taatatgaaa aaggaggata
     1261 agggattcga acccttgcac ggttttaccc gcctgacggt tttcaagacc gttcccttca
     1321 gccggacttg ggtaatcctc catatgaaac ttacaacgcc acaatggacc ttgtaggact
     1381 cgaacctacg accggacggt tatgagccgt ctgctctaac caactgagct aaaggtccag
     1441 gctcttaaaa atcgcggcgg aggggatcga acccccgacc tcccgggtat gaaccggacg
     1501 ctctagccag ctgagctaca ccgcgaaaaa gatattcatt atttaaatga aatggagcct
     1561 agcgggatcg aaccgctgac ctcctgcgtg caaagcaggc gctctcccag ctgagctaag
     1621 gccccgtgtt taaatttata attatcggga agacaggatt cgaacctgcg accccttggt
     1681 cccaaaccaa gtgctctacc aagctgagct acttcccgtt taattaacgc gcccaagagg
     1741 agtcgaaccc ctaacctttt gatccgtagt caaacactct atccagttga gctatgggcg
     1801 catatttgaa atgccgagga ccggaatcga accggtacgg tgatcactca ccgcaggatt
     1861 ttaagtcctg tgcgtctgcc agttccgcca ccccggcgcg attgctttgg caaagcggaa
     1921 aacggggttc gaacccgcga cccccacctt ggcaaggtgg tgctctacca ctgagctatt
     1981 tccgcgtgtt taaagatgcc ggctaaagga cttgaaccct cgaccctctg attacaaatc
     2041 agatgctcta ccaactgagc taagccggca tgtaaaactt aaattatgcg ggtgaaggga
     2101 cttgaacccc cacgccgtaa ggcgctagat cctaaatcta gtgcgtctgc caattccgcc
     2161 acacccgcaa aaatatgagc cgtacagggc tcgaacctgt gaccctctga ttaaaagtca
     2221 gatgctctac caactgagct aacggctcat atggaggtta acgggatcga accgctgacc
     2281 ctctgcttgt aaggcagatg ctctcccagc tgagctaaac ctccatggat aactt
//
LOCUS       DAXWOO010000245         2280 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.251, whole genome shotgun sequence.
ACCESSION   DAXWOO010000245 DAXWOO010000000
VERSION     DAXWOO010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.251"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..420
                     /locus_tag="ACGE70_002819"
     CDS             <1..420
                     /locus_tag="ACGE70_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HFF8694375.1"
                     /translation="LISGKYDYAFDFEGEKDYKSVAVFQSDFEKLKNYAGRRNVNFAR
                     LLEEFYSEELGVGNEYKRETYAKLSHKLSQALKEVTEVKAELDAKLEEVKKYQTERKN
                     NMKNQVEFDEQKEFEKARKRYNKIMNELSYEHLTVHE"
     gene            457..624
                     /locus_tag="ACGE70_002820"
     CDS             457..624
                     /locus_tag="ACGE70_002820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694376.1"
                     /translation="MVNEVETLLASFFENGHVNDEWRKEEPKRVNAYIARLKRFIDKY
                     KENSDKFSKDK"
     gene            644..2272
                     /locus_tag="ACGE70_002821"
     CDS             644..2272
                     /locus_tag="ACGE70_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694377.1"
                     /translation="MPEIEGIFSPKIGKKQLDFSSFPSVNRDYNLGDEVQILNIDFKE
                     LDFAVAVSNKPKALERVKKIYQENDQNIKNMIKKYRRDNSKVEDVLFNGTAKHDLNVY
                     MALGVALYCNSINQTDILVNIVRNSYQKILKISSETNPYYAMKYLGDNLLNDKKLMHN
                     TEELLSNLAAINFLFKTGSEDYFIDNVLKGAYGTAFYPLYNMKQHKAEQKDNKLEIKK
                     MKELLGIGFVKPNSKVTIASLATTNNDGKIVNGFDDSDIDILSKSYFSLYGDFADLMG
                     IDSYLVNNFEIKGSELNDIVYFVLISLYAIDQNTEYRQIDYLLVFYSLLYQFVLTRLF
                     KNAKEELILNSDYEIAKQIASIRKENKSEVIKLRKELSIASKENGRLESLVKSAKKEE
                     ELLKQEIKELKGIRKELEQLRFENKVLKQLQEQEHRQSLNGKTEEPSLSEMIAYLKHK
                     NILLVGGHVNFQNKLTELLPNIKMFDVSENNGKILKNLNTCDAIFLYTDYLNHGLYYR
                     VMSEISNKDKIFYLDNHVNMNLTIKRIYDLLSLI"
BASE COUNT          910 a          267 c          432 g          671 t
ORIGIN      
        1 ttgatttcag gaaagtatga ctatgctttt gattttgagg gtgaaaagga ttacaaaagt
       61 gttgctgtct ttcaatctga ttttgagaag ctaaaaaact atgctggcag gagaaatgtt
      121 aattttgcaa gactactaga agagttctac tccgaagagt tgggagtagg aaatgaatac
      181 aagagagaaa cttatgcgaa attgtctcat aagttatccc aagcgcttaa agaagtgaca
      241 gaagttaaag cggaactaga tgctaaactt gaagaagtga aaaaatatca aacagagagg
      301 aagaacaaca tgaagaatca agtggaattt gacgaacaga aagaatttga aaaagcaaga
      361 aaacgctata acaaaattat gaatgaactt agctatgaac acttaacagt tcatgaataa
      421 ttgagtgagg gtacagaaaa atatacgatc gcagatatgg ttaatgaagt ggaaactctt
      481 ttagcttctt tctttgaaaa tggtcacgta aacgatgaat ggcgaaaaga agaacctaaa
      541 agggttaacg cttatattgc tcgattaaaa aggtttattg ataaatataa ggagaatagt
      601 gacaaatttt ctaaagataa ataaggagag agtgtgatta catttgccag aaatcgaagg
      661 aatattttcg ccaaaaatag gtaaaaaaca gttagatttc agcagttttc cgagcgtaaa
      721 tagagattat aatctaggtg atgaggtaca gatactaaat attgatttca aagaactaga
      781 cttcgctgtt gctgtttcca acaaacccaa ggcattggaa cgagtgaaaa aaatatacca
      841 agagaatgat caaaatatca aaaatatgat caaaaagtat agacgagata attcaaaagt
      901 tgaagacgtt ttattcaacg gtacggcaaa acatgatttg aatgtttata tggcattagg
      961 agtggctttg tactgtaatt caattaatca gacagatatt ttggttaata tcgtgaggaa
     1021 tagctatcaa aaaatattaa aaataagtag cgaaacaaac ccttattatg caatgaaata
     1081 tcttggggat aatttattaa atgataaaaa attgatgcac aacactgaag agctgttatc
     1141 taatttggct gcgattaact ttctattcaa aacaggttct gaagattatt ttatagataa
     1201 cgtactaaaa ggtgcttacg gaacagcatt ttatccgctt tacaatatga aacagcataa
     1261 agccgaacaa aaggataaca aacttgaaat taaaaagatg aaagaactat taggtatagg
     1321 atttgtaaaa cccaattcaa aagtcactat tgcttcgtta gcaactacta acaatgatgg
     1381 gaaaatagta aatgggtttg acgattcaga tatagatata ttgtcaaaat catatttttc
     1441 tttgtatgga gattttgcag atttgatggg tattgatagt tacttagtca ataattttga
     1501 gatcaagggt agcgagctga atgatattgt gtatttcgtt ttaatctctc tatatgcaat
     1561 tgatcagaat accgagtacc gacaaattga ctatttgcta gttttttata gtttgttata
     1621 tcagttcgtt ttaactcggt tgtttaaaaa tgctaaggaa gaattgatcc tgaattctga
     1681 ttatgaaata gcaaaacaaa ttgcaagtat tagaaaagaa aacaaatctg aagtaatcaa
     1741 attaagaaaa gaattaagta ttgcgagtaa agaaaacggt cggttagaat ctttagtcaa
     1801 atcggcaaaa aaagaagagg aacttttgaa gcaagagatt aaagaattaa aaggaatcag
     1861 aaaagaactt gaacagctga ggtttgaaaa taaagtatta aagcaattgc aggagcaaga
     1921 acatagacaa tcattaaatg gtaaaactga agagccttca ctgtctgaaa tgatagctta
     1981 tctaaaacat aaaaacattc ttttagtggg aggacatgta aattttcaaa ataaattaac
     2041 tgaactatta ccaaatatta aaatgtttga tgtaagcgaa aataatggaa agatacttaa
     2101 aaatttgaat acgtgtgatg cgatttttct atatacagat tacttgaatc atggtctata
     2161 ttatcgtgtt atgagtgaaa tttctaataa agataagata ttttatttgg ataatcatgt
     2221 gaatatgaac ttaacaatta aacgtattta cgatctttta tcacttatat aaagagagaa
//
LOCUS       DAXWOO010000246         2271 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.252, whole genome shotgun sequence.
ACCESSION   DAXWOO010000246 DAXWOO010000000
VERSION     DAXWOO010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.252"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            192..446
                     /locus_tag="ACGE70_002822"
     CDS             192..446
                     /locus_tag="ACGE70_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFF8694378.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            447..764
                     /locus_tag="ACGE70_002823"
     CDS             447..764
                     /locus_tag="ACGE70_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFF8694379.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1323..2258
                     /locus_tag="ACGE70_002824"
     CDS             1323..2258
                     /locus_tag="ACGE70_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694380.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          823 a          307 c          430 g          711 t
ORIGIN      
        1 ggatgttaac ggaagtgtat ggtgagtagt tcactcgtat tttgatatcg tttgtatatg
       61 ttttcagcgt tctaagtggc ttattttgtc caataataat atggagtgga atcccttaag
      121 aaacaccgtt ggcaattgtt gttataacat gttataattt attcatcaaa aactaaaagg
      181 agtgtgataa catgggaaaa gaactaaagg ttagaaagat cggcaatagc gtgggagtga
      241 tccttccaag ctctcttgga ttaaaaagcg gagacattat ccaagccaaa caagaaggca
      301 acttattcat attagatact actcaaatag caaaagaaca cgatcgcaaa ttgattgaag
      361 aaagtttcca agactttgag aaaggtttaa cagtttcaga aattgaaatg gtaaaagctt
      421 tcggaaaata cggctggagt gaatagatga aagagtacac gctagtttat tctaaaagtt
      481 ttcagaccag ccttgaaacg ataatagaag aatgggaaaa tgagcttgct ctttctccag
      541 ccagcataaa aagatttgta ggtgcgatcc agaaggcttt agaactaacc aaaaattttc
      601 caactatgca tgaggaagtt tccgaaatct atggaatgga tatcccaaca tatcggatcg
      661 tgatcggcaa aagctatgcc ttattttatc ggatcgacca tgaaaaaaaa gagatacttg
      721 taggacgtat tttccaatct aaacaaatga aactaggctt ttaacaataa ttaggaaaaa
      781 gtcgctcatc ctctacaggt gggtgattaa tgccgctcga tatggttgtt aaccgtcggt
      841 tactcgaaga ttgatatata agacttagta cctttttatt ttgctccatg ttatggccga
      901 aggtctaaag atacaatcat tagtctagag aaaaattaag aaccgtagga actacgggga
      961 tcgcttggtg aataagagac actgctatta gtgaaaaaat aagatatcaa gtacgctcta
     1021 ttcccaaaaa tttcgtgact ttactcatga gaggttcaag tagtatattt ttaatcaaaa
     1081 ttgacgtaac ttttaataaa tactacctaa ataaggtagt atttattttt gtgttttaaa
     1141 agaggaagtc caatatatct atgggaaact tcttaaatat tatgttggta catgaaaata
     1201 tacatccctg ctagactatt tacaacattt tactttgtac gtattcttgt tttaaaagga
     1261 atgggataaa atataggtac aataactatt tgatgctaaa tataattaat ggaggagaga
     1321 tagtgaacat acttaatgaa aaaagatatg tatggtttgc tggtaaaaaa tatattacta
     1381 ctttcaaaag attatccgag ttaatgcagc agactgttaa aagtgctatg cctgaaggat
     1441 acaagtttaa ttttgttcaa tcttcagtat ctactgaaag tatctatttt tcaattcttt
     1501 ctgagagtag gctttttcat tttagaataa gttctcatgt aaagaaaact gatgttgata
     1561 tatatacgtt ccgtacaaat aattataaaa atttaaatga tttgagtaga agtatcagtg
     1621 aatatctcaa ggggaatagc tatctcaaga ttcgattttc acatatatta attttactac
     1681 catatttgaa tgagaatttc accctaattc aagtagataa gtctttcatt gcctcaggta
     1741 atcaaggaaa tatgaatatt acaagtaaca aaacgtcacg tgtgctattg gcgttgatgg
     1801 gttacggaat tctttataga gatgaccaaa atggccagat tctaataaat cgggctgggc
     1861 gttcattatt aatgaaatat agaaaattta taccggaaga ttttcaaaac ttacagttag
     1921 aaaacatgcg tttagaagat ttatttagat tagcagaaga tgataagtat agatttgaag
     1981 ttttgaaaat tcaaaagaat gaactattga ataagttata tcctgagaat gcgataaaag
     2041 cacattttgg tgatgctaat catatgtata gttggttcat tcctaaaaga tttagaggga
     2101 aaatcaatgc tggagatcaa gtgaaagtac tcaatcgaaa tagtacagat gtcgtaatga
     2161 taacagatat ctatgtagaa gatgaagata taatcaaata tatgaagcct gtgataaaac
     2221 ttgttaataa aaataaagga acccgtggtg ctagttaatt ttttcctaga g
//
LOCUS       DAXWOO010000247         2249 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.254, whole genome shotgun sequence.
ACCESSION   DAXWOO010000247 DAXWOO010000000
VERSION     DAXWOO010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.254"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(<1..392)
                     /locus_tag="ACGE70_002825"
     CDS             complement(<1..392)
                     /locus_tag="ACGE70_002825"
                     /inference="COORDINATES: protein motif:HMM:NF026247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsG family protein"
                     /protein_id="HFF8694381.1"
                     /translation="MDTEASLEINQEKSVSQKKWSTSNKILSGVLLLIMWIIMAFNTQ
                     NADYYAYSVLYNGIAQFGMDFSSDSIEPGFRAVMYVCTFISSQYQIFLLIISTVLLSA
                     LLYVIRLFTTNVFLVLFLFFIFGFTSQA"
     gene            complement(364..1260)
                     /locus_tag="ACGE70_002826"
     CDS             complement(364..1260)
                     /locus_tag="ACGE70_002826"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFF8694382.1"
                     /translation="MKNKIGIIIVTYNPDNNIKLLVDTLIQEKQNKLIIVDNGSDNIN
                     SFQFEMMENLILKKMNENIGLATAQNIGIEIAKKEKCDYILLLDQDTIIQKNLSLKLL
                     EEFTNIEAKDKDIGIIVPNYFENSNDESVSFTCKNERGRYVKKHFASTSGSMKIAFAN
                     SSGMFFKSELIDDVGNMKDEFFIDQIDVEFCKRVANNGYNIYVTNQVYFYHHVGDGEN
                     LKIGSKKIKIDNHNSTRKYFIARNSTLVKRMYKSDKEFSRLMNKRLLKFFIAIAFETD
                     KLSRFRAAIRGVRDGYRGVIGD"
     gene            complement(1250..>2249)
                     /locus_tag="ACGE70_002827"
     CDS             complement(1250..>2249)
                     /locus_tag="ACGE70_002827"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694383.1"
                     /translation="HMTIFNHMPFFTGYKKNISVMRILSHIWFSIKTLLFIRKEKPEI
                     IYCRIPPNTLVFFIAKFSRCNRVIFDVYDLWPESMPGIGNKYKFFTDLWKKLRDQYLS
                     NSSLVITECDYFKKFIKISGESKNMTIYPCSFNVDDQNDYGEKIVNKSLESTLNLAYL
                     GTINNIVDLEAINELLKTCKRKFGNKINLYFIGKGESKTKLADICEKNHIEFVDCGVI
                     YDNAEKKVILDKCHFGLNLFKSNTIIGLSLKSIEYFRFGLPIISNLSGDTKEIVKRYN
                     VGINYNSTVDENEMLKFYQCNKNINENVRKIYATIFSEQVLKMKWNEVYKVMNDEK"
BASE COUNT          757 a          387 c          263 g          842 t
ORIGIN      
        1 attgcttgtg aagtaaaacc aaaaataaaa aacaagaaca aaactaaaaa aacattagtt
       61 gtgaataatc tgatgacata caataaagct gagagtaaga cagtagaaat aatcaataaa
      121 aaaatctgat actgactgga aataaaggta catacgtaca taacagctct gaatcccggt
      181 tcaatactat ctgaactaaa gtccatacca aattgtgcta tcccattata caagacagaa
      241 tatgcataat aatcagcgtt ttgtgtgtta aatgccatga tgatccacat tatcaaaagt
      301 agcaccccag acaaaatctt gttcgatgta ctccattttt tttgtgaaac actcttttct
      361 tgattaatct ccaatgacgc ctctgtatcc atctcgaacc ccccttatag ccgctctaaa
      421 tctactgagc ttatcagtct caaaagctat tgcaataaaa aatttcaata atcttttatt
      481 cataaggcgg gaaaattcct tatcactttt atacattctt ttcaccaatg ttgaatttct
      541 tgcaataaaa tactttcgtg ttgaattatg attgtctatt ttaatttttt ttgatcctat
      601 ttttaaattt tctccatctc caacgtgatg ataaaaatag acttgatttg ttacataaat
      661 gttatagcca ttatttgcaa cccttttaca aaattcaaca tcaatctgat caataaaaaa
      721 ttcatccttc atatttccaa catcgtcaat taattcactt ttgaaaaaca ttccagagga
      781 atttgcaaat gctattttca tggaaccact agttgaagca aagtgttttt ttacgtatct
      841 ccctctctca tttttgcagg taaaagagac agattcatca ttgctattct caaagtaatt
      901 gggaacaata atcccaatgt ccttatcttt cgcttcaata ttggtaaact cttctaataa
      961 ttttaaagaa agattctttt gtatgatggt atcttgatct agtaaaagga tataatcaca
     1021 tttctctttt ttggcaattt caatacctat gttttgagct gtcgcaagcc caatgttctc
     1081 attcattttt tttaaaatta agttttccat catttcaaat tgaaaagagt taatattatc
     1141 tgagccgtta tccacgataa taagtttgtt ctgtttctct tgtattaacg tatccactaa
     1201 taactttatg ttattatctg gattgtaggt aacaataatt atcccaattt tatttttcat
     1261 cgttcatcac cttgtacact tcattccatt tcattttcaa tacttgctca gagaatatag
     1321 tggcgtaaat ttttctaaca ttttcattta tgtttttatt acactgataa aatttcaaca
     1381 tctcattttc atcaacggta ctattataat tgattccgac attatatctt ttaactattt
     1441 ctttcgtatc tcctgataaa tttgaaatta taggtaatcc aaacctaaaa tactcaatcg
     1501 atttcagact taaaccaatt atggtatttg acttaaataa atttagtcca aaatgacatt
     1561 tatcgagtat aacttttttt tctgcattgt catatataac accgcagtca acaaattcaa
     1621 tatgattttt ttcacaaata tcagctaatt tagttttgct ttcacctttt ccaataaagt
     1681 aaagatttat tttgtttcca aattttcttt tgcatgtttt caataattca ttaatcgctt
     1741 ctaaatctac gatattatta atcgtaccta aataagcaag gttcaacgta gactctaagc
     1801 ttttattgac tattttctct ccataatcat tttggtcatc tacattgaaa gaacatggat
     1861 aaatggtcat attttttgat tctccggaaa tttttatgaa ttttttgaaa taatcgcatt
     1921 ctgttataac tagactagaa ttactgagat attgatctcg tagctttttc cataaatcag
     1981 taaaaaattt atatttgttc cctattcccg gcattgactc tggccataaa tcatacacat
     2041 caaaaataac tcgattgcat ctactaaact tagcaataaa aaaaactaag gtattaggtg
     2101 gtattctaca ataaataatt tcaggctttt cttttcgaat aaataataat gtttttattg
     2161 aaaaccatat atgagataaa attcgcatta cggatatatt ttttttatat ccagtaaaga
     2221 aaggcatatg attaaaaatc gtcatgtga
//
LOCUS       DAXWOO010000248         2140 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.255, whole genome shotgun sequence.
ACCESSION   DAXWOO010000248 DAXWOO010000000
VERSION     DAXWOO010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.255"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            559..1758
                     /locus_tag="ACGE70_002828"
     CDS             559..1758
                     /locus_tag="ACGE70_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFF8694384.1"
                     /translation="MRDQTSGKMIRFYYTSLHFLYWMMFCSVYGFATLFLIYFKIDPA
                     KIGGILALVNIVSTVLQPFVSQVIIKKRKIPLITVLKTMIAGLGVILLAGVLIPNLPI
                     ITYILGGILVVSMQSFLNALAFEYSNTGYRINFGFSRAGGSFSYAVTSFLLGQLLAIY
                     SAAILPILVIVEAVIFFVILHLLPDVKNQEVFPMTKEQDRSVSLRKKYPFLILLFLGF
                     SFLFTFHTMINSFLAQIFENIHGGSKEVGIALMIAALCELPGMIFFEQLVKKKSSKFW
                     LSVASISFAVRGFMMLLASSILMMEMTHLLQAFSFALFIPASAYLFNQFLADDDRVFG
                     QTMIIIATTLGGVIGNVLGGSLLQHFGVWEMLIFGTGFAVLGALLTILGIRKIPKTGN
                     DSDSLVE"
BASE COUNT          656 a          337 c          404 g          743 t
ORIGIN      
        1 ataaagatca aactgaaatg atggatgtct ttcaagaact tgatatccat tccaaaatta
       61 ctttagatca aagttcggaa gaaaaaagag cagttgtaaa aaaagataat gaatgattct
      121 actaccgaaa ttatgatttc tttatcacga ataatgaaac ttgttttaat gaatcatatt
      181 gattgaagta tacaatgtat attaagtttt ggaaagaaat atattggtac aagatatagc
      241 aggaatcgat tactcgatcc ctgctttttt taacttcctt tgcagcaaaa aaagctcgta
      301 atctaagaga tgatggatag tttttagaat atatatattt tcttcaattc cagaaaaaaa
      361 tgaccaagct agtatgctaa aaaagtacga tgcttctatg atcttttttc cgtctttatt
      421 aacagatgtt ttaattgatc aacgcatatt gtaatctacc ctaatttcca tagttcttca
      481 ccacttttaa aaatagtaaa acccctacaa gtatgctata gtaaattgct aaagaaaaac
      541 caataaggtg ggagatgtgt gcgcgatcaa acaagcggaa aaatgattcg tttttattat
      601 acaagtttgc attttcttta ttggatgatg ttttgttctg tctatgggtt tgcgacattg
      661 tttttgattt atttcaagat tgatccagct aaaatcggag ggatcttagc attggtcaat
      721 attgtttcaa cggtgcttca gccttttgtc agtcaagtga tcatcaagaa aagaaagatc
      781 ccactgatca ccgtgctgaa gacgatgata gcaggattag gagtgattct cttagctggg
      841 gtattgattc cgaatttgcc gatcattact tatatcttgg gtgggatttt agtagtttct
      901 atgcaatcat ttctcaatgc gctggcattt gagtattcaa ataccggcta caggatcaac
      961 tttggctttt caagagctgg tggttcattt tcttatgctg taacttcttt tttactggga
     1021 cagctgttag cgatttattc tgccgcgatt ttgccaatct tggttatagt tgaagcagtg
     1081 atatttttcg ttattttgca tctgctgccg gatgtaaaaa atcaagaagt ttttcctatg
     1141 acaaaagaac aggatcgctc agtttctttg agaaaaaaat atcctttcct gattttgtta
     1201 tttttaggtt tcagttttct ttttactttc cacaccatga tcaacagctt tttggcacag
     1261 atctttgaaa atatccacgg cggcagtaaa gaagttggca tcgcattgat gatcgccgct
     1321 ttatgcgaat taccgggaat gatctttttt gaacagcttg tcaaaaagaa atccagcaaa
     1381 ttttggctgt ctgttgccag tatttcattt gctgtcagag gattcatgat gctgctggcc
     1441 agttcgatcc tgatgatgga aatgacgcat ctcctacaag ccttcagttt cgctttgttt
     1501 attccagctt cggcttattt gttcaatcaa tttctggcgg atgatgatcg tgtatttgga
     1561 caaacgatga tcatcatcgc cacaactctt ggtggggtga taggaaatgt cttgggcggc
     1621 agtctgctgc agcatttcgg tgtatgggag atgctgattt tcggtactgg ttttgcggtg
     1681 ctaggtgctt tattaactat tttgggcatt cgaaaaatac cgaagacagg aaacgatagt
     1741 gattctcttg ttgagtagca taaaaatact tagatactag ttttatataa ataacatata
     1801 gtgattattg gcgtaattac aaaaaacgag aataacttaa gtaagcattt ctaaataatt
     1861 gagaaacctc atctaaaaag ataagaaaat atccctaata aaacttctgt aacattttag
     1921 ataactgatt gtaatctgaa taataaacta taaggagacg gttagacggt ctcctttttt
     1981 tgcgtattat aattctagaa ttgttggaaa ttgataataa ttaacaagaa atattagaca
     2041 ggaacttcga actgcttatc acattcttat tattatatat ttgcgtaagt agtcctggta
     2101 gtctcaatgc attattctat cttgaaacta agaagttata
//
LOCUS       DAXWOO010000249         2123 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.256, whole genome shotgun sequence.
ACCESSION   DAXWOO010000249 DAXWOO010000000
VERSION     DAXWOO010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.256"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            130..885
                     /gene="fabI"
                     /locus_tag="ACGE70_002829"
     CDS             130..885
                     /gene="fabI"
                     /locus_tag="ACGE70_002829"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFF8694385.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(995..2047)
                     /locus_tag="ACGE70_002830"
     CDS             complement(995..2047)
                     /locus_tag="ACGE70_002830"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFF8694386.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
BASE COUNT          624 a          436 c          383 g          680 t
ORIGIN      
        1 tttaagaaaa aatagtttga caatcaaaac attttgacta ataattaaga aatcaagaag
       61 ttattttagc tattattctc tattttttta cgactatcaa atttttatca gaaacaaagg
      121 agagtcatta tgtcatttct agaaggaaaa aaaatcgtca ttatgggcgt cgctaacaaa
      181 aaaagcattg cttggggttg tgctgaagca atgttgaaac aaggggcaga aattatctat
      241 acttaccaaa atgaacgaat gaaaaaatca ttagtcaaat tggtcggcga agaagcattc
      301 actgtagaat gtgatgtttc tagcgacgaa agcatcgaaa aagcaatgaa tgccatcggt
      361 gaatatgctg gggagatcca cggccttgtc catgcagttg cttacgccaa caaagaagaa
      421 ttgtccggaa atgtgtctga tatttccaga gacggctatc aattagctca agatatcagc
      481 agctactctt taattgctgt atcaaaatac gcacaaccgt ttcttgcacc aaacagcggg
      541 atcgttagca tgacttatct aggttcagaa cgcgcaatcc cgaattataa catgatgggt
      601 attgcgaaag cttctcttga agctgctatc cgttatttgg cagctgagtt ctcgcctaaa
      661 ggaattcggg tcaatggaat ctcagctggg gcaatcaaaa cattagctgt cacaggtgtt
      721 aaagactatc aaaaactgat ccagttatct gaatctcgta cgcctgatca gcaaggtgtg
      781 acgatcgaag aagtcggtaa tgcttgtgct ttcttaatca gcccactttc cagcggaatc
      841 atcggtgata ttatctacgt tgacaaaggt gtccatcttt cataaacttt tgttgcattc
      901 tttaaagtaa aacatataaa aatcaggaaa aaacttttta aagtctcttc ctgatttttt
      961 ctttttacgt ttttttcaac tcattacttt gaaattattc cacgacttca atccagcctt
     1021 ctggtgcttc tatgtctcca aattgaatac ctgtcaattc atcatacaac cgttttgtta
     1081 ctggtcctac ttccgtttca ctataaaata catgaagatt gttattgtga taaatcccac
     1141 cgattggaga aataacagcc gctgtaccac atgcaccagc ttctgcaaat tgatctagat
     1201 catcaatgaa aatatctcct tcaattgctt gcagtcctag tcgttcttca gctaactgca
     1261 acaaggaata tttcgtgata cttggtaaga tagagggcga ttgcggagtg atgaattttc
     1321 cgtcttttgt aattccaaag aaatttgctg cacctacttc ttcgattttc gtatgtgtgg
     1381 ctggatcaag ataaacacaa tctgagtaat ccagttcttt tgcttctgtt cctggaagta
     1441 aactcgctgc gtaatttccg ccgacttttg ctgcaccagt ccccttagga gcagcacgat
     1501 cgtaatcaga tacaataaaa ttcgttggtt ttagtccacc tttgaaatac gctcctactg
     1561 gcgtacagaa gacgacaaat aaatattctg gagctggatg gacaccaatg ttttcaccga
     1621 ccccaatcag taaagggcgc aagtataatg tagcgcctgt tccatagggc ggcacaaaat
     1681 cttcatttgc ttttaccact tgcttgacag catcaacgaa catcttttca gggaccattg
     1741 gcatcagcaa tcgtctagca ctttgattca tccgtcgact attttgttca ggtcgaaaga
     1801 gattgacctt gccgtctttt cgacgataag cttttagacc ttcaaaacat tgctgaccat
     1861 aatgaagcgc tggagagcct tcatgaatcg tcaggtaatt atcttctgtc aattctcctt
     1921 cttcccattg tccgtctttc catttggcaa taaaacgcca aggagttttg atataagaaa
     1981 aacctagatt gttccaatcg atttgcatat acttaccacc ttcgttattt tctccacatt
     2041 ttatcattat ccatattatt tgtcatttta ttttcagctt ttttccttca ttatagttat
     2101 ttttgctatt tccttttttg aaa
//
LOCUS       DAXWOO010000250         2111 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.257, whole genome shotgun sequence.
ACCESSION   DAXWOO010000250 DAXWOO010000000
VERSION     DAXWOO010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.257"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(248..469)
                     /locus_tag="ACGE70_002831"
     CDS             complement(248..469)
                     /locus_tag="ACGE70_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694387.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(567..812)
                     /locus_tag="ACGE70_002832"
     CDS             complement(567..812)
                     /locus_tag="ACGE70_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694388.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1052..1666
                     /locus_tag="ACGE70_002833"
                     /pseudo
     CDS             1052..1666
                     /locus_tag="ACGE70_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT          714 a          365 c          304 g          728 t
ORIGIN      
        1 tttttactat atacttcttc ccagaacttg tccgtctcct tcaaattatc tctacctctc
       61 gagttcattg ggtggtttgc ccatttatta aacatggaat cacctttctc atactcatat
      121 atttatgtcc tatttcaatt atatttatat atgcttaaat aaatttgtat cctgaaattt
      181 attgtaatat gaagctgtat tttacatata gtaaccaaag agttaataaa ataaaaagag
      241 cgaaccatta tagatcagct cttttttcat tgctgttttt aatcttatct agaatcactt
      301 caaatgtttc aaaggtattt acatctaact gacataacca aagcaagtac ttcttttctt
      361 tgtaagtcca atcaatatta cggtaagagg acattagatt atggtacctt gctctttgtt
      421 caactgaagc caattcccag cgtttttcaa atattttttc ttcttccatt attgatccct
      481 cgcaagacgc tacctcaaac ttaaataatg ggcagcgaaa gtctaattcc ttagatctaa
      541 gtatagcaaa caaatatgaa aaaaacttag aaggtagtaa aagttcttcc gtcttttgct
      601 ttgcttctat tcttaatgtg tttcgttatc ggtgccctac aaagattttc attaattaca
      661 cactcattta ttgttgtgta ttttctgaca tttctctctt gatcaattat tttaattcca
      721 tgggcatcct gatagttcat aggagaggag cggctatcag aaatagttgc acctatctct
      781 tgaacttctt ttacaacttc tggaaaatcc atccatcctg cttcagctgt caaaagtaat
      841 aatctataat atttatcttt ccttttcaat ctaagaaatc gtcaccccat tctttttctt
      901 ctattataaa ctatttaact tttagaattc aaaaaccgtt tgtctattta gagtttaccc
      961 taaatagaca aaaatataac attgaaaaat ttagagttct aacgtatgat tagggtgtat
     1021 tataaacgtt atagaaaagt agggaataaa tttgaaaatt gcttatatta gagtttcatc
     1081 catcgatcaa aatgaacaac gtcaaattga agaaatgaaa aagtttggcg ctgaaaaaat
     1141 ttttatcgaa aaacaatctg gagcgacgat tagtcatcgg ccgattttcc aggaagcttt
     1201 agattttgtc agagagcaag atatttttat tgtagaagcc attgatcgac ttggacgcaa
     1261 ttatgacgag attatcgatt cagtgaatta tctaaagaag aagaatgtgc gacttattat
     1321 caccagttta ccgatcatgg ctgaagcaat tggaaatcct ctcctagata aattcatcaa
     1381 agacttaatc atttaaatct cagccatgat tgctgaacaa gaacgtactg aatcgaaacg
     1441 acgccaggca caaggaatta aaatcgctaa agcaaacggc gtctataaag gtcgtcctaa
     1501 gttatacagt gcagacacca aagatcctca acgtcgcctt gtctatagga gtatcgttca
     1561 ggatcttgag aatggggttg ctatctcaaa gattgcaaca gactacaatg tgactaggca
     1621 aacaatttac cgaataaaaa aagaaatcga ccaactaatt gtatgatttc ttttttgaat
     1681 cacgtaatta ttaattagct ttgtcatcaa tacagacaat cattttagat tcatctagct
     1741 aagttgatta catttatgtt aaaaagaatt tagttaacat aaatataatt gatgcataaa
     1801 tcgtccttat ttaaaggcat ttaacattga caacttagta taatgtatct tatgtaaact
     1861 aataagattt gaattttatt ttatacacac tcgataatag atcaatttta tggtcccccg
     1921 catttacgat tgcacaagag aaacataaag tcgacctatt agcatttctt ttcaattcaa
     1981 acattaaaaa agtttatacc tctgctacta aaaaatgaaa ttatctattt aaaatttaaa
     2041 acggtaaatc tgattcatcc aaattggaaa atggatcagc ctgtattgga gctttgtatt
     2101 tctccataat a
//
LOCUS       DAXWOO010000251         2110 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.258, whole genome shotgun sequence.
ACCESSION   DAXWOO010000251 DAXWOO010000000
VERSION     DAXWOO010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.258"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            121..2109
                     /gene="treP"
                     /locus_tag="ACGE70_002834"
     CDS             121..2109
                     /gene="treP"
                     /locus_tag="ACGE70_002834"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFF8694389.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLVYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
BASE COUNT          613 a          409 c          461 g          627 t
ORIGIN      
        1 aaaacgatat cattttctct cgtacttttt aataaaacgc ttgcaactct ctccgaaaaa
       61 aagtatagta aatgaagaaa caacttgcat agacaagtta agagaaaggg gtttattgat
      121 atggcaaaat atcaagcaga tgccgagaag ctgttacatg atatcggcgg aaaagaaaat
      181 attgctgcag ttagtcattg tgcgactcgt atgcgttttg tcctgaatga tccaggaaaa
      241 gcggacgaaa aagcgatcga agatatacct agcgtaaaag ggatgttcac gaatgcagga
      301 cagttccaag tcatcatagg aaacgatgtt tctacttttt ataacgactt cgttgcagta
      361 tctggagttg aaggggtttc caaagaacaa ggaaaagcag ctgcaaaaca aaaccttcat
      421 cccgttcaac gtgctattgc cgttttagca gaaatcttta caccactgat tcctgcaatc
      481 attgtcggtg gattgatcct tggattcaga aatgtacttg aaggtatcca gtttgaaagt
      541 cttggtggaa caatcgttga acactcgaag ttttggaatg gtgtcaatgc ctttttgtgg
      601 ttaccgggtg aagcaatttt ccattttctt ccagtaggta tcacttggag tattgcaaaa
      661 aagatgggta ctacacaaat attaggaatc gttttaggaa ttacgttagt ttctccgcaa
      721 ttattaaatg cttatagtgt agcttccaca gccgcagcag atattccatt ttgggatttt
      781 ggttttgctc aagtccagat gattggttat caagcgcaag ttattccagc catgctggca
      841 gggtttatgt tagtgtatct agaaatcttc ttccgcaaat atatccctca gtcgatctcg
      901 atgatttttg ttccattgtt ttcattgtta ccaactgttt tagctgcaca cgttatttta
      961 ggtcctatcg gctggacagt cggaagctgg atctcaaata tcgtaaatac tggattgacg
     1021 tcttctatca gctggttgtt tagtgccgta ttcggattcc tgtatgctcc tttagtgatt
     1081 actggtctcc accatatgac caatgccatc gatatgcagc tgattgcaga tttcggatca
     1141 acgaatttat ggccgatgat tgctttatca aatatcgccc aaggctctgc agtcttagcc
     1201 atcatcttct tacatcgtgg gaataaaaaa gaagaacaaa tctcgattcc agcaatgatt
     1261 tcttgttact taggcgtaac tgaacctgct atgttcggaa tcaatttgaa atacgtctat
     1321 cctttcgtcg ctgcaatggt cggctctgga ttagctggca tgtttgctaa cttgatgggt
     1381 gttcgagcta acgcgatcgg agtaggcggt ttgccaggaa tactagctat ccaagcagaa
     1441 acatgggtac ctttcatcat cgcaatgatc attgctgtca tcattccatt tggcttgacg
     1501 atcatcttta gacgtcaggg aattttgaat aagattgatc ctgctgttcc tgaaaatgct
     1561 gctgatgtac aacttcaaac agcaaatggc gcaacagcaa cgccacagtc tttcgaacct
     1621 gtttcagcaa ctggtacggc agttgcaaca aaagaaacat tattcgcagt cgctgcaggg
     1681 accatcaagg aaataactga agtaaatgac ccagttttct cacaaaaaat gatgggtgac
     1741 ggttatgctg tcgaaccgtc aaacggcaaa gtctatgcac cagtaaacgg taaagtgacg
     1801 agtgtctttg aaacaaaaca tgccattggt attctttcta atgaggggct ggaagtactt
     1861 gttcacatgg gattagatac tgttgaacta aagggtgttc ctttcaatgt attcgtgaaa
     1921 gaaggatacc ttgtcacacc agagaccctg attgctgaaa tggatttgcc agaaatagaa
     1981 caagctggca agaaaactga cattattgtt gctttgacca ataatgaaaa agttgcagga
     2041 ctttctcttg accaatcagg acttgttcgc ccgggcgaag cagtcggtaa agctgaagta
     2101 aaatcttagt
//
LOCUS       DAXWOO010000252         2102 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.259, whole genome shotgun sequence.
ACCESSION   DAXWOO010000252 DAXWOO010000000
VERSION     DAXWOO010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.259"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            715..1047
                     /gene="traE"
                     /locus_tag="ACGE70_002835"
     CDS             715..1047
                     /gene="traE"
                     /locus_tag="ACGE70_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFF8694390.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1666..2007
                     /locus_tag="ACGE70_002836"
     CDS             1666..2007
                     /locus_tag="ACGE70_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFF8694391.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT          739 a          278 c          436 g          649 t
ORIGIN      
        1 aatttataag ttaaaatatt atgataaaaa tcacaaacgt agccttttac gtcataagtg
       61 ttaaaagaaa taattaattt caaatataga aaagggaacg tatgtttgca tactcctttt
      121 ttttgagtta taataatacg cgaagagaga taatttcatt atttaaacaa gctaaaaatc
      181 ttgatattac ttcttttcag gcaatacgaa tattattcgc ttacattttt tgtataaatt
      241 taattgtctg ctatgcttgt gatgaattga ttttcgtttt gcacttttgg atgataaatt
      301 tggacaattg aaaatacaaa aaaaagaaag gcggtatgaa ctcggaaggc tggtaacctt
      361 cgtcagcgtt catacacggc attaagattt ggcccttaat gcgttaacct ttcagtgtta
      421 cggtattacc cgcaatggat ttagtataac agatattcaa aagtgatacc actttttttg
      481 gctgtgttaa ggttaaaaat acgtgatatt acgatgaatc tatgcctaag cataatgtta
      541 taaaaatgtc cgtgtggctc tttgccgtat gacgaacgct gaaattagag ggaacgcatg
      601 tttggatgcg ttccctcttt tttgtttgtc atgaacgaaa atcaattctc tatttttgaa
      661 aaataacaag aaaaacttgt taaaaatatt taaagaaatt gggtggttta ttttatgtca
      721 tatacaggaa ttttgagtct caaagatatt tgtcactacg gtaaacggtg taccgctaca
      781 gagaaaatca cgaagaaact atcaactggc caaagtaaaa ccgttgtgca gtgtaagaaa
      841 tatataatcc agaaagacaa agtctcagag gaaatgattt attacattgg gaaacaaaag
      901 caaataattt taaaagatcc tatcccctta aaagagctat atccaacaat caaacatgtt
      961 tatgaccaaa acggtgtact gattggtcga agaaaaaatg gagtcttgcg ttgtaccgca
     1021 aaagggatgg ggcgtttgat cggctgaatt gatcattctc ataacttgta ttttatcgtt
     1081 tttcgcttgc ttgaaaatat tgtcgagact tctaaaggaa cttagagtca ctagaaaaat
     1141 aaggagtaga cttgatctat caacaattcc cttcggggag tgacggggta ggttttaacg
     1201 attggtaacg taaaaaattg gtcaatcgag taaatgcgct tgtttggata gtggtgaaat
     1261 gaatttgtgt aaataataag cgcagagtaa acaagaggga atttagaagt tagacaagtt
     1321 agaatagcgt ctagattaat catgagcagc tactaaggga tctagacttt gacgcaaact
     1381 catggaataa ggagtaggtc ttctcgtaat ggggaggacg cacaagaaat tgtgtcgtgt
     1441 gctgcagtac atgtagatac ttgcgaggac gttgattatt ggatcaatcg agcatttacc
     1501 gctgtctact agggtttctc gtttcggacg ccataaacga gtcgaaattt tttatgtatt
     1561 tttaagtaaa cgaaaaacga taaaatatga acaagtcctg aataaagtga aaaaattaat
     1621 ataaagcata gagaaaatga aatttacgta atggaggaat gacaaatgaa aaattatgat
     1681 ccgaatatac gattgggcac acatactatt aaggtttctt tccaacgttg ggattacaaa
     1741 ggatttgtga cgtttagaag gggcggaaat tgtaaaggat tagatgtttt agcattagac
     1801 gaggatgatt tatacgatca aacattgact gataatccaa ttggattcgg cttgctacct
     1861 gaagatgatg aaggtaatga atggtttaaa atgacgctta tgaatgataa tggagacgaa
     1921 ttatctgttg aagatacttg gtcatatttg agtgattata ttgtcagctt tgaaatcatt
     1981 gaatttgtag cggataaaga agaataaaaa ctggaggaat aaattttaca gataaaggct
     2041 tagaaggagt cacgacgatt aataaaaagg tagtattacc ttaggcattg agtaaggaat
     2101 tt
//
LOCUS       DAXWOO010000253         2087 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.260, whole genome shotgun sequence.
ACCESSION   DAXWOO010000253 DAXWOO010000000
VERSION     DAXWOO010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.260"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            84..407
                     /locus_tag="ACGE70_002837"
     CDS             84..407
                     /locus_tag="ACGE70_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFF8694392.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            589..1959
                     /locus_tag="ACGE70_002838"
     CDS             589..1959
                     /locus_tag="ACGE70_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFF8694393.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
BASE COUNT          587 a          385 c          427 g          688 t
ORIGIN      
        1 cctcgttgaa gtaagtattt ctagtttatc ctaggattaa ttataaaaat aaaacattcg
       61 ttgattcgaa ggaggttaaa cagatgtttg aaagttttga tagcagccgc aaccgttacg
      121 cttcagttgg tgtcgtatca agtttgccag atgaattgat tgacagtata tggtttatta
      181 ttgacctaga tcttaaggga gtcattccat tagaaaatat attaaccttt gatttgatcg
      241 aaaatcgtgg aaaagtgacg atgcattttt ctcaagcagg aagcaatgtg gaaatggata
      301 ttgatttgcc gtttgcctat tcgaaccaat ttcctgctca agtatacgcc tatgatgacg
      361 gtacgagaga aacgatcctt ttaccaagtg agattagaca atattaaggt gatagataaa
      421 cgtggatgtc ccttatttta cagaccaggc caagttgttg attctttgac tcaattcttg
      481 gcttcttttt atggagtttc ataaattatc attgatttct aattggtatt ctcaggataa
      541 acgctatata ctactaacta aattttaaag atataaggag tttaaaagat gcgcgattta
      601 acaaaaggaa gccccgctaa actgattttg atgttcactg ttcctttact gatcggaaat
      661 gtttttcagc agttctacaa tatgatcgat atgatcatcg tgggtcagac gctaggtaaa
      721 aatgcgctgg cagcggttgg tgcaactgga agcctgacat ttttgatcat tggctttgcc
      781 caaggtttaa cagctggttt agccattatt accgcacaac gttacggtgc aaaagattat
      841 cggggtctga agaaaagttt tgctgctagt gttgtcatta gtcttgtcgt cactgtgatt
      901 ttaactgtcc taagtctcct gtttattcgt ccgatgcttc aattgatgca aactcctcct
      961 gaaatacttg atcaagcaca aacatttatt tcaattattt tattagggat ctttgcatca
     1021 gtcagtttca atttgctttc taatgtcatt cgcgcacttg gtgacagccg cacaccacta
     1081 ttctttctga tcattgcggt catcatcaac gtcgtacttg atttgatctt tattatctat
     1141 tttggcatgg gtgtggaagg tgcagctatt gctacagtaa tcgcacaagt ttcttccagc
     1201 gtactgtgtc tagtgtatat caagaaaaaa atgcctttgt tacagttacg caaaaaagat
     1261 ttcaaatttg aaaaagaaga gtttgctgta cacctaaatg ctgctttgcc aatggcgttt
     1321 caatcttcga tcatcgctgt tggtgctatc gtgttacaag cagcactaaa tagtctaggg
     1381 acagatgtcg tagctgctca agccgctgct ggtcgtatcg accaatttgc aaatcagccg
     1441 atgatgtcat ttggtattgc aatggcaact ttcactgcac aaaactatgg tgcgaaagaa
     1501 tatggccgaa tcttgcaagg ggtacgtcaa actttactga tgagtgtcgg atttagtctc
     1561 ttagcaggtg ctatcgtcat tttcttcggt catttctttg ttcaattgtt tgttagccca
     1621 tcagaaacac gtgtatttga attagcacaa acctacttca atatcaatgg gtctctttac
     1681 tggatcttag cagtcctgtt cattttacgc tacacacttc aagggcttgg acaaagcaag
     1741 atccctacga tcgccggtat gatggaattg cttatgcgtt cttttgcagc tatcgttctt
     1801 acaggaatgt ggggataccc aggagcagca gcagcttctc ctttagcttg gatcggttct
     1861 gttgccgtgt tgctttactc ttatatgcgt tcgatgaaac aattgaaaca catgggtgaa
     1921 gaggaacatt tcacattaga acatgcagaa gcaagatagc cttgattaaa agaaatcgtt
     1981 gcggctaaaa atgaaaactt ttattgaaaa tatcttcttc gtttaccgaa aacaggtttt
     2041 ttagataaca aaaaggctgt gacaaaaaat gtcacagcct tttttct
//
LOCUS       DAXWOO010000254         2079 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.261, whole genome shotgun sequence.
ACCESSION   DAXWOO010000254 DAXWOO010000000
VERSION     DAXWOO010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.261"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            20..2077
                     /locus_tag="ACGE70_002839"
     CDS             20..2077
                     /locus_tag="ACGE70_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HFF8694394.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYTVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVNILKEQIVNVTHGDSVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFATPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
BASE COUNT          748 a          382 c          382 g          567 t
ORIGIN      
        1 aaatttagga gttgaaaaaa tgaaattatt taatccaaaa actgttttag ctattggaac
       61 atttgctgcc tcacttagtc ttgctggatc tgttgacgct gcttcaacta acgtcatgta
      121 tcgcctttac aatccaaata ataatgaaca tttttacacc tctagcaaaa aagaacgtga
      181 ccatctagta aagataaaat ggggaaatta tgaaggcccc gcctgggaag caccgactag
      241 cggtggccat cttgtctatc gtttgtacaa taaaggtctt cgtgatcacc attacacagc
      301 tagttgggat gaagtgaaat ggttgactaa aaattacggc tggacttatg aagggcctgc
      361 ctggcgttct gccgaaaaaa ataataaacc gatctaccgc cttttccttc ctggtgtaaa
      421 atctggctca catcactaca ctgcaagttg gaaagaagta caatggttaa ccaaagacta
      481 tggctggaaa tacgaaggta tcggttggta tggcgctgac acctctaaac ccaaagtgaa
      541 taaatcggct ctagaagctc tttatacgag cgttaaggga acgaaaaaag gtgactatac
      601 cgacaatacc tggaacgcat tccaaaccgc tttaaacaat gctaagaaag tgttaaatga
      661 ctcaaaagcg actcaaaagc aagtggatag tgccaaaaat aaattagatt ctgcttacaa
      721 aggattaaag aaaaaaccag ttcctactgt gaataaagct gaattacaag ctttatacaa
      781 ccgagtaaaa tcaactgcta aaggagattt tacagaagaa tcttggaata attttcagac
      841 tgctttatcc aatgctaaaa aagtactaga cgattctaag gtttctcaaa cacaagtaaa
      901 caatgctaaa aatagtcttg atgctgcatt taaagggttg caacacaaac ctgaaacacc
      961 aaaagaatat acggttacta ttaaccatat tgacaatcaa acaggtcaag tgattgctac
     1021 agatcaagca aaagtaactt ctggttctac ttacactgct aaggcaaaag catttaaata
     1081 tagcgaaggc gctaaagata attttagcta caaagtgatt ggtaatgata ctcaaagcaa
     1141 aacaatcaca ggtaatacaa caatcacatt caattataat caagtgcatc aaatcaattt
     1201 gttgtgcaat aacaactaca aaaacgaagg acgagttaat attttgaaag aacagatagt
     1261 caatgtcact cacggtgaca gtgtcacttt aactgctcct tctattcaag gatatgtttt
     1321 agacgatcgg gtagaaccaa caaattcagt aaccttaaac aatgtgactg aaagtcaaaa
     1381 ttatattttc aactacactc gtaaatttaa tgtgactgtt aatcatgtaa atgctgatac
     1441 caacgctgtt ctctctagtg aatcaaaaac agtatacgaa ggggatagct ttgctactcc
     1501 ttggaaaaac atgactgatc aaaattattt cttgtgcaga aatgacgatc ctaccgttac
     1561 cgtggataaa aacggccaac gatccattaa taacgtcgat caaaataaaa caatcacatt
     1621 caaatataaa aagatttcat tagacgaatt acgtaacttt atgcgtcaaa aagaacttgc
     1681 ttggttaaat gattatcgcc aacaaaatgg tgttgcccca atgcaattca atgatattgt
     1741 tcaacaggct gcagatattc gagctaaaga attacaagtc agcttcagtc actatcgccc
     1801 tggtggtggt actttccaag atttattgga aagcttaggc tgctacggtg ctaagggaga
     1861 aaacataagt caaactgggc tgtatattga tgacctttta tcagacgatg gtgctatcga
     1921 tgccatggta ggatggaaaa atagccctgc ccataatgca aatttacttt ataataacca
     1981 aagtattctc ggattaggtc ataattttgt agttgactct gctggtagaa ttggcgtaga
     2041 taatgtattt attagtgcga atccaatctt caaataaaa
//
LOCUS       DAXWOO010000255         2059 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.262, whole genome shotgun sequence.
ACCESSION   DAXWOO010000255 DAXWOO010000000
VERSION     DAXWOO010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.262"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            143..832
                     /locus_tag="ACGE70_002840"
     CDS             143..832
                     /locus_tag="ACGE70_002840"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFF8694395.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            846..>2059
                     /locus_tag="ACGE70_002841"
     CDS             846..>2059
                     /locus_tag="ACGE70_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFF8694396.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEE"
BASE COUNT          642 a          284 c          410 g          723 t
ORIGIN      
        1 tcaacttttc tccattatac tccaaaataa taaaaaaaac tcctaaatac tccattttta
       61 tctttacttt tatgaaagcg gatgctatat tgtttttgga gttattatcc aaaaataata
      121 aaaaattgga gcgtttagga taatggataa aaagaggttt ttagatctta tttcaaaagg
      181 tttgattgtt tcatgtcaag cattacctgg tgaaccattt tacaaagagg aagaaagttt
      241 aatgccttat tttgctttgg ctgctcaaag ggctggagca gttggtatac gtgcaaattc
      301 tgttcgagat attattgaaa ttagaaagtg tgtatcatta ccaatgattg gaattattaa
      361 aagagattat ccaccagaat ctccattcat tactgccact atgagagaag tagatgaatt
      421 agtagaaacc ggtgttgagg taatcgctct tgattgcact cttagaaatc gacatgatgg
      481 actatctata aattctttta ttactaaaat aaaagaaaaa tatcctcatc agttatttat
      541 ggcagatatt tctacatttg aagaaggcaa aaatgcatat aaagcaggaa tagattttgt
      601 aggaactact ttaagtggat atactgagga aagtaaaaaa caagatgggc cagatataga
      661 tttgattaaa aaattggtag aagaagggat ttgtgtaatt gctgagggaa ggattcatac
      721 tccagaacaa gcaaaagaga taaaaaaaat aggagttgca ggtattgttg tgggtggtgc
      781 tattactcga ccacaggaaa ttgcatcaag atttgttaaa gcattaagtt agggggagaa
      841 ataaaatgtt taaaaaattt tcacaattag gacgcgcttt catgttacca attgctatat
      901 taccagtagc cggattacta ttgggactgg gaggagcgtt aacaaatgag agtgctataa
      961 atgcttatcc atttttagat caaccgtggt tgcatacaat tttaagtatt atgagttatg
     1021 ctggaaatgc agtttttgca aacctagcac taatttttgc aattggtatt gctgtaggac
     1081 ttgcaaatgg agataaagga acagctggtt tagctggtgg agtagcttat ttagtatata
     1141 ctgcaactat tagtgggttt cttgaacttt tttcagcaaa agatgcaaca ctagatacag
     1201 gtgtagtggg atcaattgtt gttggtggta ctgttgcatt tttacacaat cgttatagaa
     1261 aaattgaatt accacaattc ttaggttttt ttggaggatc aagatttatt ccgattgttt
     1321 ctagtttagc tgcaatcatt attggttctt tcttttattt aatttggcca ccaattcaaa
     1381 atggtttagt agttgctggt gaacaaattg cccaaatggg tagtttagga acttttttat
     1441 atggtttttt attgcgttta acaggtgctg ttggactaca ccacaccatt taccctttgt
     1501 tctggtatac atctttaggt ggtaccgaaa ctgtagctgg atcaacaatt gctggagccc
     1561 aaaatatttt ctttgcccaa ctagctgatc caaaccacac tggtctgttt acttatggta
     1621 ctagattttt tgcaggaaga tttgcaacaa tgatgtttgg gttaccagca gcatgttatg
     1681 caatgtatcg tgcaattcct aagaaaaatc gtaaaaagaa tggtgggctt tattttagcg
     1741 gggcccttac atcattttta actggaatta ctgaaccagt tgaatatatg tttttatttg
     1801 ttgctccttg gctttatgtg attcatgcat tcttagatgg attatctttt tattttgctg
     1861 atatcttaaa tattcgtgta gggaattcct tttcaggtgg tttgatcgac tttttattat
     1921 tcggtgtact tcaagggaat gacaaaacta attggttaaa attaattcct tttggtattg
     1981 catgggcatt tatatatttc tttgttttta cattctttat caaaaaattt aaggtagcta
     2041 ttcctggaat ggaagaaga
//
LOCUS       DAXWOO010000256         2001 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.263, whole genome shotgun sequence.
ACCESSION   DAXWOO010000256 DAXWOO010000000
VERSION     DAXWOO010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.263"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..376
                     /locus_tag="ACGE70_002842"
     CDS             <1..376
                     /locus_tag="ACGE70_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFF8694397.1"
                     /translation="NVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFRR
                     FPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTDD
                     WQKVMAAEVIATYLDGDLVYKK"
     gene            424..1824
                     /locus_tag="ACGE70_002843"
     CDS             424..1824
                     /locus_tag="ACGE70_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFF8694398.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
BASE COUNT          553 a          339 c          432 g          677 t
ORIGIN      
        1 aaatgtttcc acagaggact tctttaatta ttgtcgatta gaggaaagtg gtgtaacagt
       61 cactagcggt acagatttac cgctgtttat tactgatatt cctgaatcga ttataagagc
      121 agtattccgt cgttttcctg aaggagaaga agtctgggag aaagaaacaa gcttcacgac
      181 aaccgagctg ctgaaaagtt ttacaagcaa cagcttcaaa gtaaatggtt tatcggagta
      241 cggagaaata aaagtaggga aaagcgccag cttagctgtt ttcagcgagg acctctgtac
      301 tgatgactgg caaaaagtga tggctgcgga agttatcgct acctatcttg atggtgactt
      361 agtttacaag aaataatgct tttcgatata acgttataaa acgaattaaa gagaggttaa
      421 aaaatggaaa caatgaaaag aaaattcacc ttcccacata gttatacatt gatttttatc
      481 ttaattattt tggctgcaat tttaacttgg ataattccta gtggtcagtt tgagacagtt
      541 gttgaaaacg tcgcaggtat ggaaaaaaca acggttgttc cgggaaccta tcaccaaatc
      601 gataaggcag attatggaca aggtttttcc tctatactaa tggcaccagc tgaagggatt
      661 atttcagctg ttgaggttgt tgcttttgtt ctcatcgttg gtggtgcctt cgggattatc
      721 ctaaaaacgg gagccatcga tcgagggctg tttaccttag caaaagttct gggagacaag
      781 gggatcttag ttattccgat gtcgatgatt ctattcagct taggaggatc aacctttggc
      841 atgagcgagg aagtcattcc cttatttgcg gtgtttattt ctctgatgtt ttctcttggc
      901 tatgattcca tgactgcgat cttgattcta tttttaagta cacaagcggg atatgttggg
      961 tcgacgatta atcccttcaa cgttttaatc gcacagggag tagctggcgt ttacggaaat
     1021 cccctgcttt ggtttcggat gctatcctgg attgtcatta ctgggatttc aattgttttc
     1081 accatgcttt atgctagcag agtcaaaaag aatccactgt cctctattgt ttatgagagt
     1141 gatttaaagg ttaagcataa gtttcaaaat tttgacactg aaaaagagtt ttcaacacaa
     1201 gataaactaa ttatcgccgg ttttatcgta ggtttaggaa tcatgatttg gggtatcatt
     1261 tccaagggat ggtatatggt tgaaatttct gcactattct taggattagg tctttttgct
     1321 gggggcatcg ctagattttc tcaaaaggaa attgctgaag cttttgtcga aggctgttca
     1381 gatttcgcct atgctgctgt aatcattggg ctagctagag gagtcttggt aatcttggaa
     1441 ggtggaatga ttattgacac tattctaaac agcttggcta accttttagg tggtcttcca
     1501 aaattcctgt tcactacgat tctactcatt tctcaagtaa ttgtttcctt ttttgttcca
     1561 tcctcctcag gagctgcggc attaacgatg ccggttatgg caccattggg tgatctaatg
     1621 aatgtaagtc gtgatgttgt tgttctaagt aaccagtttg gaaatggttt aatgaatatt
     1681 atctccccaa ctagtggtgt attactggca ggattagcca tcgcagagat taatttctct
     1741 aaatggttta aggttggctt caaaatattt gccatcttaa cccttgtttc tgctgttttg
     1801 ttatacattg ctacactctt ttagtcttaa tcatccatta ataggaggca atagttatga
     1861 ttaaaataaa tcaattagca ggaatgaata tccactattt attttattct ctcaaagaat
     1921 ttttcgctta tcaacagaaa attggtattt cttctgttga actttgggga ggtgctccgc
     1981 attttttcat agatgctttt a
//
LOCUS       DAXWOO010000257         1995 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.264, whole genome shotgun sequence.
ACCESSION   DAXWOO010000257 DAXWOO010000000
VERSION     DAXWOO010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.264"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            128..1852
                     /locus_tag="ACGE70_002844"
     CDS             128..1852
                     /locus_tag="ACGE70_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFF8694399.1"
                     /translation="MKKKLLTLFLVMSFSIVLSVYYNTIVFSEQINYTEEQYNIEKEK
                     ALKVGYSEEQFKQIMEIPTNLSNENSETRIVNYTMTSNQTKVINKAMEQIGKPYEWGA
                     SGPNSFDCGGLVKYVYKQAVNIELPMGTTNQEQYGTEVSLNSLKPGDLLFYGNRGATY
                     HVGIYKGNGVMIHAPQPGETVKEVNIQYFYPNFAKRILPDEPDYPYIDYNKMVTVTKA
                     WSIWNDLQFSHEIKKAIIGDNYKIGKVYTNPENNNKYGEILVSNKVYGYINFYAVKEL
                     TSVQINRYLTSKDSGQPTWGNLECTISKGQTTKDKIYFVKGAYNLGDGKYLYSIYKDQ
                     DSSEWLGYLKAHVSLAYTPIEEINKNVTVTKNWSIWNNLQWEKEIEKPQIGSVFSARL
                     KLTNVSNNAVYYKLYKSGKFYGYINAEAVKDLTTTKLNKYVTFSVNNEDFWSSLDVNY
                     SKGKTERGRVFYISISYNTADNQPIYSVYTDETCTEWRGYYKGNNFEDTQITMLENKS
                     VKVTKSGYTVWGDLNFWTKSGISNTGDIYTTDRKFYNFTNNAYYYELKKDGRVYGYIN
                     SEAAVEMN"
BASE COUNT          783 a          242 c          348 g          622 t
ORIGIN      
        1 cagtttgtaa caaacatata ttttttatat tacaatattt caacaaatta aaaaaaatta
       61 tatcttctta aacaatgata atattaaaat gcaagcgctt gtaaaaaata aggagaagag
      121 gtaaaaaatg aagaaaaaac tattaacttt atttcttgtg atgtctttct caatcgtttt
      181 gagtgtgtac tacaatacta ttgttttttc agaacaaata aactacacag aagaacagta
      241 taatatagaa aaagaaaaag ctttaaaagt tggttatagt gaagaacagt ttaagcagat
      301 tatggaaata cctacaaatt tatcaaatga aaatagtgaa acaagaatag taaattatac
      361 tatgacttct aatcaaacca aagtcattaa taaagcaatg gagcaaattg gaaagccata
      421 cgaatgggga gcatcgggac caaattcttt tgactgtggt ggtttagtga aatatgttta
      481 caaacaagct gtaaatattg aactgcctat gggaaccacc aatcaagagc aatacggtac
      541 agaggtaagt ttaaattcac ttaagcctgg tgatttatta ttttacggca atcgaggtgc
      601 aacttatcat gtaggtatat ataaaggtaa cggtgtaatg attcatgcac cgcaacctgg
      661 agaaactgta aaagaggtta acatacaata tttttatcct aactttgcaa aaagaatttt
      721 gcctgatgaa cctgactacc cttatattga ctataataaa atggtaactg ttacaaaagc
      781 ttggtcaata tggaatgatt tacaatttag tcatgaaatt aagaaagcta ttattggaga
      841 taattacaag atagggaagg tttatactaa tccagaaaat aataataaat atggagaaat
      901 attagtaagt aataaagtat atggatacat taatttttat gctgttaaag agttgacctc
      961 tgttcaaata aacagatatt taacttctaa agatagtggt caaccaactt ggggaaatct
     1021 agagtgtact ataagtaaag gacaaactac aaaagataaa atatattttg ttaaaggagc
     1081 atataattta ggggatggaa agtatctata ttctatttat aaagatcagg attcctctga
     1141 atggttaggg tatttaaaag cacatgttag tttagcctat actcctattg aagaaattaa
     1201 taaaaacgta actgttacta aaaattggtc gatttggaat aatctacaat gggaaaagga
     1261 aattgaaaag cctcagattg gttctgtatt ttcagctaga ttaaagttaa ccaatgtatc
     1321 gaataatgct gtctattaca agctttataa atctggaaaa ttttatggtt atatcaatgc
     1381 tgaggctgtc aaagacctta caacaacaaa gcttaataag tatgttacat ttagtgttaa
     1441 taatgaagat ttttggtctt ctttagatgt aaattattca aaaggaaaaa ctgaaagagg
     1501 ccgagtattt tacattagta tttcatataa taccgcagat aaccaaccga tttattcagt
     1561 atatacagat gaaacatgta cggaatggcg agggtattat aagggaaaca actttgaaga
     1621 cactcaaatt actatgcttg agaataaaag cgtgaaagta acaaaaagtg ggtatacagt
     1681 atggggagac ttgaattttt ggactaagtc aggaatatcg aacacaggag atatatacac
     1741 cactgaccga aaattttata attttaccaa caatgcttac tattatgaat tgaaaaaaga
     1801 tgggagggtc tatggctata taaatagtga agcagctgta gagatgaact aaaatattgc
     1861 tgtcccttaa tcttatgggg cagcaatatt tttatattat taatcatttt tctgaagcga
     1921 gggtagaaga attaatcaat agtttttctt gttttgtaat ataatagttt aattaccaaa
     1981 aattatttaa gtaaa
//
LOCUS       DAXWOO010000258         1976 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.265, whole genome shotgun sequence.
ACCESSION   DAXWOO010000258 DAXWOO010000000
VERSION     DAXWOO010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.265"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            42..1208
                     /locus_tag="ACGE70_002845"
     CDS             42..1208
                     /locus_tag="ACGE70_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HFF8694400.1"
                     /translation="MKISVFGLGYVGLANSLLLAQKEQVKAYDIVEEKITMLQNGISP
                     IEDKEVHEFLKRDDLNVEFTSNFTDAVNFGDYLIIATPTDYDEKKNYFNTSTVEDVIE
                     KALAIRPDSTFIIKSTVPVGYANEIKAKFGTENIIFSPEFLREGKALYDNLYPSRIIV
                     GECSARGREIATMFQRNAFNKDASVLLTHSTEAEAIKLFSNTYLALRVAYFNELDTYA
                     EFRGLDSKQIIEGVGLDPRIGSHYNNPSFGYGGYCLPKDTKQLRANYEKVPSNIISAI
                     VDANTTRKDFIAGQILAKKPNVVGIYRLTMKAGSDNFRYSSIQGIMKRIKAKGIEVVV
                     YEPTLDEDEFYHSQVLKDINEFKNISDVIVSNRFEDILKDVEEKVYTRDIFGDN"
     gene            1461..1604
                     /locus_tag="ACGE70_002846"
     CDS             1461..1604
                     /locus_tag="ACGE70_002846"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694401.1"
                     /translation="MENAITVITNSFFSSIKYLKTLFVCQADQCSFRLGRTLISSQVM
                     IEV"
BASE COUNT          683 a          278 c          383 g          632 t
ORIGIN      
        1 aatactataa aatgaaattt gattggagcg tatggaagaa tatgaaaatt tcagttttcg
       61 gattaggcta tgttggtcta gcaaattcac tattattagc acaaaaagag caggtcaaag
      121 cttatgatat cgtggaagaa aaaatcacga tgttacaaaa tggcatctca ccaatagaag
      181 ataaagaagt tcatgaattt ttaaaacggg atgacttaaa tgtcgaattt acgtcaaatt
      241 ttactgatgc agtaaacttc ggtgattact tgattattgc aactccaaca gattatgacg
      301 aaaagaaaaa ctattttaat acatcaacag tagaagatgt aattgagaag gctttagcaa
      361 ttcgtccaga ttcaacattc attattaagt cgacagttcc agttggctat gcaaatgaaa
      421 ttaaagcgaa atttggtaca gaaaacatca ttttttcacc tgaatttttg cgtgaaggaa
      481 aagctttgta cgacaactta tatccatcaa gaattatagt tggagaatgt tctgctcgtg
      541 gtcgcgagat tgcaactatg tttcaaagaa acgcatttaa caaagatgca tcagttcttt
      601 tgactcactc aactgaagca gaagctatta aattattctc gaatacatat ttggcattgc
      661 gagtggcgta tttcaatgaa ttagatacgt atgcagaatt tcgtgggctt gatagtaagc
      721 aaattattga aggtgtcggt ttggatccac ggatcggttc gcattataat aatccttctt
      781 ttggctatgg tggatactgt ttacccaaag acacaaaaca attaagagcc aactatgaaa
      841 aagtaccaag caatatcatc agtgcaattg ttgatgcaaa tacaacaagg aaagatttta
      901 ttgctggaca gattttggcg aaaaaaccaa atgttgttgg aatctatcgt ttaacgatga
      961 aggccggttc agataatttt cgatattcgt cgatccaagg aattatgaaa cggattaaag
     1021 ccaaaggaat tgaagtggtt gtttacgagc ctacattgga tgaagatgaa ttttatcatt
     1081 cacaagtatt gaaagatatt aatgaattta aaaatatttc tgatgtgatc gtatctaatc
     1141 ggtttgaaga tattttaaag gatgttgaag aaaaagttta taccagagat atctttggtg
     1201 ataactaaat aataatgaac gggtacatgg aggaaaatat tttagatggt gactcgaaat
     1261 aaatgtttgt tctcattgca tctgtatcta taccgataag tttaaaaatt ccgattagga
     1321 aaatgaagtt ggctatcctt gcgaggaatt tattgctgga ttattttatg aactaaagca
     1381 agacatcatc agaaaaaaat ccaaaatttg acaatgggat tgatcgttta ccagctgtaa
     1441 gcgaagtatc tgacacgaaa atggaaaatg caattactgt tattaccaat agcttttttt
     1501 catcgatcaa gtatttgaaa acattatttg tttgccaagc tgatcaatgt tctttcagac
     1561 ttggaagaac attgatcagc tcacaagtaa tgattgaagt ttaggttatg ggataaagga
     1621 taagtgaaat aaaagtcgtg tctgcatctt tgttagtacc agtaaatttt tctactacgg
     1681 cgctagtcac gacagtccag gcatctgaaa ttgaagatgc aaatagagtt tctgctagtt
     1741 taggtattga tgttactgta ttagcattaa tcttagagcc agtagcatcc aataggtgta
     1801 gctatagttc taatagtata aaacaatgca gaggatatga ttgctttatg tctcggtgtg
     1861 tcaggtcaaa atagagttcc atatggggcg atttctcaat gaataatctt atagatataa
     1921 tcttgtttgc tatttcgtta tcacttttac ttttcttcga ttatttcaaa aataaa
//
LOCUS       DAXWOO010000259         1971 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.266, whole genome shotgun sequence.
ACCESSION   DAXWOO010000259 DAXWOO010000000
VERSION     DAXWOO010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.266"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            6..329
                     /locus_tag="ACGE70_002847"
     CDS             6..329
                     /locus_tag="ACGE70_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694402.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            493..747
                     /locus_tag="ACGE70_002848"
     CDS             493..747
                     /locus_tag="ACGE70_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694403.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            817..1062
                     /locus_tag="ACGE70_002849"
     CDS             817..1062
                     /locus_tag="ACGE70_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFF8694404.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            1138..1503
                     /locus_tag="ACGE70_002850"
     CDS             1138..1503
                     /locus_tag="ACGE70_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFF8694405.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1564..>1971)
                     /locus_tag="ACGE70_002851"
     CDS             complement(1564..>1971)
                     /locus_tag="ACGE70_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694406.1"
                     /translation="ILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNR
                     RCMGILGRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCS
                     FMSREVGDEPKLEYAFSFAREDEDNPKTYDILA"
BASE COUNT          544 a          354 c          347 g          726 t
ORIGIN      
        1 tctcaatgtt aggagtgatt attttgccat ttattgcgat tttattcgat ttagttgcgg
       61 cagcagctta ttttctccaa ttaaattatc aaacctcagg aattctgttt attggaatga
      121 tttttcaagg tgtgattacc cttttgcttc ttgtcatgat gctcagctat aaaggaaaaa
      181 gatatgcccg tattcaaaca aaagtatttg ttaaatatgt ttccattcgc tatgggatca
      241 tcattctttc ctttttagtg aatgcgatcg tcctcttttt atatgtattg aattacttag
      301 atatcaatcc attgattttt tctcgataga taagacacat tcagcggaac agttcctgct
      361 tatcagcaga aaactgttct gctttttcat ttttcattga tctattcttc ttttattttc
      421 ctagcatttc atttgtgatg tatgcaataa catgttatct tttattatat gaatcgttga
      481 ggagtccgtt acatgattac gttggtgaga gttttgtttt gggtgcctgc ggtcgcactg
      541 gtcgcaagta ttgtttatct tatgaattgg aacaaagaac ggttttattt agctattttg
      601 actcttccgg ctatctactt tatgtggaaa gtttttaatt acaactactt tgaacccgat
      661 agtgttttta tagaagaatt atcaggactg gttttgtctt tattgatcgt cattctttat
      721 ttgatacgtt taaataaaaa acattgagta ctattgattg catgtgtagc tttctatagt
      781 cattataact attctactat aatgaaaggt gattaaatga gtatcgaaga agctatttta
      841 ctggatactt tacgttgtcg tctgaatttc ttaagaactg gccgatcttt atctcaagta
      901 gctgtttcta gcgcgttaag tattccttta ttcaaatatc gtaagtacga agaaggagaa
      961 gaaattccaa gcatcattga attaatctat atcgctgact tttacgatat ttccgttgat
     1021 tacctaattg gtcgctcaga aacagcaagc aaactgaagt gattttttcc agccttggtg
     1081 ctggtttttt catttaacgt ctaaggttaa caatctatat ataaaggaga catttctatg
     1141 cgattatggc atgaaaaatt gatttcaaag cttcctcgtc aacagctttt aggacaacat
     1201 cgggaatgct gtgcattaag aggaaatggc tggggaaaga aacatgctac tgtggattat
     1261 gtttttcact attctcctta tagattattc caatatcatc agcttgtcat ggatgaaatg
     1321 gaaaaacgtg gttacagccc agcaccagag tggaaaaatc ctatgtatcg tggcaaatca
     1381 tgcgaagcat tttttgattt acctactgta gagcgcaacg tgccgattta tccggaacac
     1441 gatgaagttt atctagcaga atgtttagca aatttggagc aaaaaggaat ttatcttacc
     1501 taatataact agaaaagcct tctatctcct cttacttcat taaaaagctt taaccatctt
     1561 agatcaagct aaaatgtcgt aagttttagg gttatcttca tcctcacgtg caaaactaaa
     1621 agcgtactct agttttggtt catcgccaac ttccctagac ataaatgaac atctcctgac
     1681 gtggtttaaa agtaccgcat cttcgacgtc tgtatacgat gcacagcaca gattgcttct
     1741 tttgatcttt cttttgattt ttctgttgct tcttctacac cttcttcgat tactacatcg
     1801 atgcttacaa ttgcttctgc ctaatattcc catgcatcgt cgatttaagc ctctgtagtc
     1861 actgtgatcg tttccacatt ttaactcctc tgtcgtggct tcagttgatt ttgtgatcag
     1921 ttcagttgct aagccagagt cagaacaacc tgccattgct agcgttaata t
//
LOCUS       DAXWOO010000260         1955 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.267, whole genome shotgun sequence.
ACCESSION   DAXWOO010000260 DAXWOO010000000
VERSION     DAXWOO010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.267"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            156..1862
                     /gene="efbA"
                     /locus_tag="ACGE70_002852"
     CDS             156..1862
                     /gene="efbA"
                     /locus_tag="ACGE70_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFF8694407.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT          700 a          387 c          353 g          515 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaa
//
LOCUS       DAXWOO010000261         1942 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.268, whole genome shotgun sequence.
ACCESSION   DAXWOO010000261 DAXWOO010000000
VERSION     DAXWOO010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.268"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            271..837
                     /gene="amaP"
                     /locus_tag="ACGE70_002853"
     CDS             271..837
                     /gene="amaP"
                     /locus_tag="ACGE70_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFF8694408.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            850..1038
                     /locus_tag="ACGE70_002854"
     CDS             850..1038
                     /locus_tag="ACGE70_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFF8694409.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1051..1608
                     /locus_tag="ACGE70_002855"
     CDS             1051..1608
                     /locus_tag="ACGE70_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFF8694410.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1640..1882
                     /locus_tag="ACGE70_002856"
     CDS             1640..1882
                     /locus_tag="ACGE70_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFF8694411.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          713 a          301 c          400 g          528 t
ORIGIN      
        1 taaatagaat cttgtaataa taactgagca taatctttgc aatttttaat cttgaataat
       61 atcctttaag tatagccagc aatcaaaagt aaatagagaa taagtaagca attgttattt
      121 cagtatcaga ctggcaataa aacgccgtta tgatccagca atgtttggta gcgctttatt
      181 tactgatgct atgctaactg ttcgctatct atactgtgat tgtcggtaga gttatttaca
      241 gggtaaaaat aagtttatag gaggtggaaa atgaataagg ggttaaaaat aatcggtgtt
      301 atcatgagtt tactgctact ttcagtattt ctagcgattg cgttgatcaa tgcgccgtat
      361 attatgcctg aacaattgga acgatttcgc ttctttactt taacgaatta ctacatgcaa
      421 caatatattt tctgggcagc tgtagtattt gccgttcttg ctattatcat cttgctagtc
      481 gttcttttct atccaaagtc aagaggaact ttcgttatga aacgggaaga tggcaaacta
      541 acaatcgaca aaaaagcgat cgaagggttg gtacgttccc atttacacga agaagagttc
      601 attcattcac ctaaagtacg gatacgttca acgaaaaatc gaatccatat ccatgtcaac
      661 ggagacttga aacgaacttc ttcattagta gggaaaacag gtgtattgat gcaagatatc
      721 gaagaagaag taactaaagt actcggaaca aaagaaacgg tcaaagtggc agtcacatac
      781 agcggttatg aagaacaaga agatacaaga gactacaagc actctcgagt ggaataggag
      841 ggccaaataa tgaaagaact attttcagca tacaagctcc ctattatctt tgggggtctt
      901 ggactactgc tagctgtttt atggttaacg attggatttt tcaagacatt gttgatcata
      961 atcatgacag caattggcgc aggaatcggt ttttatttga aacagtcagg aatagttgat
     1021 caatttttta aatcatagga ggaaacaaag atggaaaaca aaaacatgaa tacaacaaat
     1081 ggatcagttt tggcaaatga aaaaggagaa aaccgcatga tgaatggtga attgacttat
     1141 gaagacaaag tcatccaaaa aatcatcggt tatgcattga aagatgtaga tggtctattg
     1201 acattagatg gcggattttt ctcaaatatg gctgaaaaat tagtcaacac agataacgta
     1261 acagcaggta tcaatacaga agtaggtaag aaacaagtag ctgtggatct agatgtgatc
     1321 gtagaatacg gtaaagacat cgaagatatt tacaaccaaa tcaaagaatt gatcagtact
     1381 gaagtaaaca atatgacaca tttagatgtt atcgaagtga atgtgaatgt tgcagacatc
     1441 aaaacacaag aagaatacca aaaagatcaa gaaacagtac aagacaaagt aacagaagca
     1501 gcaaaatcca caggacaatt tgcttcacgc caaacagata aagcaaaatc tgctgtaggc
     1561 aaaggtgcac aaaaagtcaa agaaaataac gaaccacgtg tacaataaga agtaagaagt
     1621 cagttaggag gaatttaaaa tgggatttat ttgggcatta atcgtcggcg gtatcattgg
     1681 agcaattgca ggagcaatca cgaataaagg atcatcaatg gggatcatcg caaacatcat
     1741 tgctggttta gtaggttcag caattggaca agctttacta ggaacttggg gaccaacaat
     1801 tgcaggaatg gctatcattc catcattgat cggtgcaatc attttagtat tagtcgtttc
     1861 attctttgca cgtaaattat aagcaaattg aagaaaaact tatgttgaaa tgaagaacaa
     1921 atttgagaac cgtagcgaca ct
//
LOCUS       DAXWOO010000262         1937 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.269, whole genome shotgun sequence.
ACCESSION   DAXWOO010000262 DAXWOO010000000
VERSION     DAXWOO010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.269"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..809
                     /gene="bioB"
                     /locus_tag="ACGE70_002857"
     CDS             <1..809
                     /gene="bioB"
                     /locus_tag="ACGE70_002857"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFF8694412.1"
                     /translation="KSGLCNEDCGYCAQSKKSKATIQKYSLLPQDEIIRKALIAKKNN
                     SSVFCIALSGTRPTQKEVLVLGETIKEIKRIMDIEICLSIGLITDNQIRYLKKCGIDR
                     LNHNLNTSRENYPNITTTHSYDDRLNTLKKIKAFDINICSGFICGMGETDTQLVDLAF
                     ELKEQEPFSIPVNFLLPIKGTKLEKYNELTPIKCLKILIMLRLLFPKTEIRISAGREF
                     HLGSLQQFAILIVDSIFLGNYLTEKGADVTEDYQLLKELELIVEGDYNEY"
     gene            799..1464
                     /gene="bioD"
                     /locus_tag="ACGE70_002858"
     CDS             799..1464
                     /gene="bioD"
                     /locus_tag="ACGE70_002858"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFF8694413.1"
                     /translation="MNISMVICGIDTNCGKSVASILIYEYLKLYFSVNPIIVKPVQTG
                     QELDTIFYQQCDIPQKNIFNFYSLDIEASLHTAGNLTNQKIDLQSLLDQSKALLSDNR
                     PKIFELAGGILSPINDKETMLDFVKLLNLPIILVVDNYLGSINHTLLTIHLLESKNIE
                     ILGLIYNNTESRNYSTELFEYITNNTCIPQIGKIPYLKNLKYVLKSEKEKQNLIREWH
                     LNV"
     gene            1457..>1937
                     /locus_tag="ACGE70_002859"
     CDS             1457..>1937
                     /locus_tag="ACGE70_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010709177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFF8694414.1"
                     /translation="MYRKSQMIARMSLMLALLIITARLVIPLPLFDYLSLQIITVYLI
                     YPTLGMKNGLIVTCSYLILGIIGLPIFASGGGYAYIFKPTFGFLLAFVTLPIIQSFIS
                     NKFNNAIYSSFKKNAIINICCLLYIYLIGIIYKILILSFYTNNVTSLSILISITTLID
                     "
BASE COUNT          709 a          267 c          280 g          681 t
ORIGIN      
        1 ctaaaagtgg attatgtaat gaagattgtg gttactgcgc ccaatctaaa aaaagtaagg
       61 cgaccattca aaaatacagt cttttacccc aagatgagat tatacgtaaa gcattgatag
      121 caaaaaagaa taattcaagt gttttttgta ttgcgttaag tggaactaga cctactcaaa
      181 aagaagtatt agttctcggc gaaacaatta aagaaattaa gcgcattatg gatatcgaga
      241 tttgtttatc tattggatta atcacagata atcagataag atacttaaaa aaatgcggaa
      301 ttgatagatt aaatcataat ttaaatacta gtagggaaaa ctatcctaat ataactacta
      361 ctcattccta tgacgatcgt ttaaatacgt taaaaaaaat caaagctttt gacataaata
      421 tctgctctgg ctttatatgt ggaatgggag aaactgatac acaattagtc gatttagctt
      481 ttgaattaaa agaacaagag cctttttcaa ttcctgtaaa ttttttatta cccattaaag
      541 gaaccaaact tgaaaaatat aatgaactaa ccccaataaa atgtctaaaa attctaataa
      601 tgcttagact attgtttcca aaaacagaaa ttagaataag tgctggtaga gaatttcatt
      661 taggcagtct tcagcaattt gctattttaa ttgtagattc tatattttta ggaaactatt
      721 taacagaaaa aggcgcggat gtcacagaag attatcaatt attaaaagag ttagaactta
      781 tcgtagaggg ggattataat gaatattagt atggtgattt gtggaataga tacaaattgt
      841 ggtaaatcag tagccagtat tttaatttat gaatatctga aactctattt ttcagttaat
      901 ccaattattg taaagccagt gcaaacagga caagagcttg atactatttt ttatcaacag
      961 tgtgatatac ctcaaaaaaa catttttaat ttctattcat tagatatcga agcctcacta
     1021 catactgcag ggaatttaac aaatcaaaaa attgacttac aatccttgtt agatcaatca
     1081 aaagctttac taagtgacaa tcgacctaaa atatttgaat tagctggtgg aattttatct
     1141 cctatcaacg ataaagaaac aatgttagat tttgtcaaat tattaaacct tccaattatc
     1201 ttagttgtag ataattactt aggctcaatt aatcatactt tactaactat ccatctttta
     1261 gaaagcaaga atatagaaat tttgggattg atttacaata atacagaatc tagaaattat
     1321 tctactgaat tgtttgaata tattactaat aatacttgta ttcctcagat tggtaaaatt
     1381 ccttatctaa aaaatttaaa gtatgttttg aaatcagaaa aagaaaaaca aaatttaatt
     1441 agggagtggc atttaaatgt atagaaagag tcaaatgatt gcgcgaatgt cattaatgtt
     1501 ggcgctgtta attattacag ctagacttgt tattccttta cctttatttg attatttatc
     1561 tttacagatt attactgttt atctcattta tcctacatta ggtatgaaaa acggtctgat
     1621 cgtaacctgt agctacttaa ttctaggaat aataggtctt ccgatatttg caagcggtgg
     1681 tggatatgca tatattttca aaccaacttt tggtttcttg ctagcctttg tgactttacc
     1741 tattatacaa tcgttcatct caaataaatt taataatgct atctactctt cttttaaaaa
     1801 aaatgctatt ataaatattt gttgcctact ttatatttat ttaattggaa ttatttataa
     1861 aatattaatc ttgtctttct acactaataa tgtaaccagt ctttctattc tgatcagtat
     1921 tactacatta atagatt
//
LOCUS       DAXWOO010000263         1921 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.270, whole genome shotgun sequence.
ACCESSION   DAXWOO010000263 DAXWOO010000000
VERSION     DAXWOO010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.270"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(308..381)
                     /locus_tag="ACGE70_002860"
     tRNA            complement(308..381)
                     /locus_tag="ACGE70_002860"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(345..347),aa:Ile,seq:cat)
     gene            complement(570..1589)
                     /locus_tag="ACGE70_002861"
     CDS             complement(570..1589)
                     /locus_tag="ACGE70_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFF8694415.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1600..1806)
                     /locus_tag="ACGE70_002862"
     CDS             complement(1600..1806)
                     /locus_tag="ACGE70_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFF8694416.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT          565 a          390 c          328 g          638 t
ORIGIN      
        1 gctttttaat ggcttcaaat ataaaagagt ttaaagcgta acacacttat ggggaagtgg
       61 tttggggtgc gctttaaact cttctttatt attatctcac aatttaaccc aaatgtcttt
      121 ctatttaaaa atcaaagtaa aacttttcaa atatacagaa gtataactat gtgaaacatc
      181 ctttcattaa tccataaaag gatacataaa aaagccactc atttgagtgg caatgaagaa
      241 aagctttagc ttgtataata ctcttcaaaa aaattctaac acagaacgat tcaaatggct
      301 acgttaatgt accctgtagg actcgaacct acgaccggac ggttatgagc cgtctgctct
      361 gaccaactga gctaagggta ctggttgttg ccacataaag ccataaacaa tcaaccagta
      421 gaatgtgtgg caacaaacct gttatcgcat agcttggagt gtgactatta tgggtgatag
      481 tgaagatatg cgataacatc actattttat cgaatgattt ttatagttgt caatatagtt
      541 atgtaatgct cctcaacgag gagctatttt tatcgtttag gaatatttaa ataccaacgt
      601 ttgtcatgga aatcttgcgc accgccttta gtgtttccct ctggatcatt cgttgcccgc
      661 atcatgacat agactttctt attagggaag ttacgcatgt taaaagatac atgatagcca
      721 acatttccag aagtattata agcttgattt acatctgatc tataaattcc atcagctctt
      781 actcgagcta attctttccc agtattgtaa tccataatga agatatactc gtatttatag
      841 ttagcaatgt gccatccagc tacatgcaaa tttgcatttt cgatttctcc aaactgatca
      901 atgtgggcgt aattcgttcc atctgtcagt gtaggattag ctgcaccagc tcgtgttgga
      961 tcaatgacag gcttgttttc agaagttgtt ggattttcat cggtaaatcc atgagctaaa
     1021 tcatatgcta atttttcttt acttacgccc atttcagaaa gataaccgta aggatctgta
     1081 tgatcgcccc agatgttttg tgttacccat aaatgcgatt tgattcccgg ttggttataa
     1141 ggagtgtcca acgttaatgg aataccatat ttcattgctg aatctctagc caattcaacg
     1201 tatgccttgt agtttttctc aaacgttgct ttatcatgtg tgtgttgtaa ctcaatctgc
     1261 acaggactgt tggcattagc atacgaacca gcaccgtact gtacataacc aggttgaccg
     1321 acttgataaa caattccgcc gtctcccaca atataagcag tataagcgct agtccatgaa
     1381 cgttgcatat actgcgcttc attgcgtcct gttgctgttt cattagccgt ttcatgcagt
     1441 aaaatatact gattatttgc tacttgtgag ctaccttcat ttgcgcccaa attaaattca
     1501 ttgttgatag tataggcaaa tccgttaatt ggcaataaaa aaagagccgt taacaggctc
     1561 atcgcagtaa tagtaatttt ctttttcata tctatcctcc tatttatctg atgaaatttt
     1621 cttatacgtt cgatttgata cacctaaaat actccctaga aatgctccaa aacctgtaat
     1681 aatcatcacc gttatatctg tgtaccgcca gttaacacct tttccgacaa gaccgacaaa
     1741 agtcgctaaa gcggggataa ctactatagc gatccatttc aaaatatcaa aggttctatt
     1801 gttcatacta tccacctccc ttcattggtt gggctacttt ctttttcaat cgcttgtttt
     1861 cttctatcaa tatttctctt tctgaaagca aggcatcaat ttgcccgtcc agttgttcaa
     1921 t
//
LOCUS       DAXWOO010000264         1911 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.271, whole genome shotgun sequence.
ACCESSION   DAXWOO010000264 DAXWOO010000000
VERSION     DAXWOO010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.271"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(407..1510)
                     /locus_tag="ACGE70_002863"
     CDS             complement(407..1510)
                     /locus_tag="ACGE70_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFF8694417.1"
                     /translation="MANKNGYDIEMSYVVAPGETLKEVAEELGLSHKDLAERLGITPK
                     TISKIVNGTAPITPETALKLERVLGISSNFWNQLELNYREKLTEIEAEAKLKDEIEIL
                     KEYIPYNEIANAGFVPKTRNLKEKVENTLNYFGYSSLKIFIESVNDNQLLAGAYRIKE
                     VDKVNKLALLAWIRAGELEAQEIEAEIFSKEKLKNSLSELRNLTLQSNPSIFIPKLQK
                     ICASFGVKVVFIPEVKGSKVCGLTRWLTPKPQAIIQLSLRYKSNDNLWFTFFHELGHI
                     LKHNKIPFYTSSNEYIDSIEEQEADEFSANTLIPRDSYNDFVEQGCFTRNSICNFAEM
                     ISIHPGIVLGRLQKEKYVQWNQYNDLKTWYSWK"
     gene            complement(1500..1829)
                     /locus_tag="ACGE70_002864"
     CDS             complement(1500..1829)
                     /locus_tag="ACGE70_002864"
                     /inference="COORDINATES: protein motif:HMM:NF016875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFF8694418.1"
                     /translation="MIVRARKRKLQKILADSKLINLEFGKQIGDKVKQRIAELLAAET
                     LEDISHLPPTKLHSLSGEYEGCFAVKLTGNFRLVFKAYTCEEEFTTQKSNTEIIVIEE
                     VVDYHGK"
BASE COUNT          575 a          374 c          271 g          691 t
ORIGIN      
        1 aagctttagc ttgtataata ctcttcaaaa aattctaaca cagaacaatt caattggcta
       61 cattaatgta ccctgtagga ctcgagccta cgaccggacg gttatgagcc gtctgctcta
      121 accaactgtt aatcagtgtt ccaaagggag tgattatact ctgctatttt caaactttct
      181 taaatcgtct atgataatct cagtttatgg tggcaaaatg gtggcaagaa aaaaagtgag
      241 tatgcccgct acttgcgcaa tcggagtact gctcgatcat ttatttggaa acaggctaca
      301 cttgagggtt tggaagagtc aaagcggttg agcggtgaac ggaaaaagaa cttaactacg
      361 tatagttagg ttcttttcgt ttatgtttta tcggtagaat aattttttac ttccaagaat
      421 accaagtctt caaatcatta tattgattcc attgtacata cttttctttt tgcaaccttc
      481 ctaaaacgat ccccggatga attgagatca tttccgcaaa attgcaaatt gaatttctag
      541 tgaaacaacc ttgttcaaca aaatcattat agctatctct aggtattaat gtattagcag
      601 aaaattcatc tgcttcctgt tcttctatac tatcaatata ctcatttgaa ctagtataaa
      661 aaggaatttt attatgcttt aatatgtggc ctaactcatg gaaaaatgta aaccataaat
      721 tatcattaga tttgtatctt aaactaagtt gtattatagc ttgcggtttg ggggttagcc
      781 accttgttaa cccacacact ttactccctt ttacctctgg aatgaatact accttcactc
      841 caaaactagc acaaatcttc tgtaatttag gaataaatat actaggattg gattgcaaag
      901 taagatttct caattcagat aaagaatttt ttaacttttc tttagaaaaa atttctgcct
      961 caatctcttg agcttctaat tctccagctc taatccacgc caacaaagca agcttattta
     1021 ctttatctac ttctttaatt cgatacgctc ctgctaacaa ttgattatcg ttaacacttt
     1081 caataaagat ctttaatgaa gaatacccaa aataattaag cgtattttcg actttttcct
     1141 taaggttcct agttttagga acaaatcctg catttgctat ttcattatat ggtatatatt
     1201 ctttcaaaat ttcgatttca tctttcaact tggcttctgc ttcaatttct gtaagcttct
     1261 ctctgtagtt aagctccaat tgattccaaa aatttgaaga tatgcctaaa actctttcta
     1321 attttaaggc tgtttcaggt gtaattggcg cagttccgtt aacgatttta gaaatagttt
     1381 tcggtgtaat ccctaacctc tcagctaaat ctttatgaga taacccgagt tcttcagcta
     1441 cttctttcaa tgtttctcct ggagcaacta cataggacat ttctatatcg tacccatttt
     1501 tatttgccat gataatccac cacctcttca attactatta tttctgtatt acttttttgt
     1561 gttgtaaatt cttcttcaca tgtataagcc ttaaaaacta gcctaaaatt acccgtcaat
     1621 tttacagcaa aacacccctc atattcccca gagagagaat ggagtttcgt gggcggaaga
     1681 tgcgaaatat cttctaatgt ttctgccgct agcaattcag caattctttg cttaacttta
     1741 tctccaattt gttttccaaa ttctaaatta attagcttgg aatcagctaa tattttctgt
     1801 aattttcttt tcctcgctct aactatcatc cgacttaacc ttttaggtta acaactctaa
     1861 atatagtata ccacaataat ttgtttttca agcactttat gatttactta a
//
LOCUS       DAXWOO010000265         1880 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.272, whole genome shotgun sequence.
ACCESSION   DAXWOO010000265 DAXWOO010000000
VERSION     DAXWOO010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.272"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            180..1730
                     /locus_tag="ACGE70_002865"
     CDS             180..1730
                     /locus_tag="ACGE70_002865"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFF8694419.1"
                     /translation="MSDKNTYACRSQRHKQESDTYENPDQLNETVTSKTANENKQSRI
                     PKKNSNYSKYIFALFTALILVTVATAGYIVYKLKQQEAEAKAKYETAAKNLMASIQEE
                     QDQSRISTKIDTISDGENKCLVYRPVDESAVPFKNANQLLDELAQKQQKKHCEKEVLT
                     VARIKATAISSKVGQYSIEADSFIWDRSKEHFKNPDNISEKAIYVSEKTGKEITNKDL
                     IPDEGSLLGIQQVIQQSILDHAEKPEKIIDAVLTMDRISYDSKMTYTPDELTIILPNN
                     SIGTSKVTLNYKDIAPFIDTDLVSQESIKDALPALDENKKYVALTFDDGPNNSSTLDL
                     LNILKTDNVKATFFMLGQMVDQNPDVAKQAHDEGHEVACHSYSHPQLNALSTDELRSE
                     INEANKAIFKATGVLPRNIRPPYGAIDKKSAETIGMPIIQWDIDSQDWKSKNPRTINN
                     VVKQNVYSGAIILIHDIHPESVKAIPNMISMLKNEGYEFVTIDQLLSGKQKTLHQYFG
                     MNDERLVD"
BASE COUNT          734 a          300 c          329 g          517 t
ORIGIN      
        1 aaataagaga ggaagaagaa atatgtaaaa aaacctttta aattggtgta cgtataaaat
       61 gctgagaaaa catctataat tactacggca aaagtaaaaa aatagtgaaa aatgccaggt
      121 aaaaaataag aaatttctat gttttattta tatgatgata aatggaggaa taaggccata
      181 tgtctgacaa aaatacctat gcatgtcgga gccaacgtca caaacaagaa tctgatacct
      241 atgaaaatcc agatcaattg aatgaaactg taacgtccaa aaccgcaaat gaaaacaaac
      301 aatcaagaat accgaagaaa aattcgaatt atagcaagta tatattcgca ttgtttaccg
      361 cacttattct agtaactgtt gctactgctg gctatattgt ctataaatta aaacagcaag
      421 aagcagaagc taaagcaaaa tatgaaactg ctgcaaaaaa tctcatggct tcaatccaag
      481 aagagcaaga ccaaagtaga atttcgacga aaatagatac tataagtgac ggagaaaata
      541 agtgccttgt ttatcgtcca gttgatgaaa gtgctgttcc ttttaaaaat gcaaatcaac
      601 tcttagatga gcttgctcaa aagcaacaaa agaaacattg tgaaaaagaa gtccttactg
      661 ttgccagaat aaaagcaacc gcaatctctt ctaaagttgg tcagtatagc attgaagcag
      721 atagttttat ctgggatcgt agtaaggaac attttaaaaa tccagacaat atttctgaga
      781 aagccattta tgtttccgaa aaaactggca aagaaattac aaataaggat ttgattccag
      841 atgaaggaag tctcttaggt attcaacagg tgattcagca atccatactt gatcatgccg
      901 aaaaacctga aaaaattatt gatgctgtat taacaatgga tagaatttct tatgatagta
      961 aaatgactta tacacctgat gaattgacta tcatcttacc aaacaattca attggaactt
     1021 ctaaagtcac tctaaattat aaagatattg cgccattcat tgatacagat ctggtttcgc
     1081 aagaaagtat aaaagatgca ttacctgcgt tagatgaaaa taaaaagtac gtcgctctca
     1141 cttttgatga tggtccaaat aatagctcaa cacttgattt gttgaatata ctaaaaacag
     1201 acaacgtgaa ggctactttt ttcatgttag gacagatggt ggatcaaaat ccagatgttg
     1261 cgaaacaagc tcacgacgaa ggtcatgagg tagcatgtca ttcgtattct catccacaat
     1321 tgaatgcact ttcaacagac gaattacgaa gtgaaataaa tgaagctaat aaagcaattt
     1381 tcaaggcaac aggtgtcttg cctcgcaata tccgtccccc ttatggcgct attgataaaa
     1441 aaagtgctga aacaattggt atgccaatta ttcaatggga tattgactca caagattgga
     1501 aatcaaaaaa tcctagaaca atcaataatg ttgtcaaaca gaatgtttat agtggtgcaa
     1561 tcatcttgat ccacgacatc catcctgaat cagtcaaagc gataccaaat atgatctcta
     1621 tgttaaaaaa cgaaggatac gaatttgtga cgatcgatca actattgtca ggaaaacaaa
     1681 aaacattgca tcaatatttt ggtatgaatg atgagcggct agtagactaa ctgttattag
     1741 attgcttggt atgtgtagaa gttatacttg caattctctc tcagaaaaaa tgttaagcaa
     1801 aacaaattga taaagagtat cgagaatgct aattcttatt agtcaaagtt ctattttagt
     1861 aaataaaaaa agtactggaa
//
LOCUS       DAXWOO010000266         1840 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.273, whole genome shotgun sequence.
ACCESSION   DAXWOO010000266 DAXWOO010000000
VERSION     DAXWOO010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.273"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            46..483
                     /locus_tag="ACGE70_002866"
     CDS             46..483
                     /locus_tag="ACGE70_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694420.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFESIEKNGAFYTIESEEKDNE"
     gene            476..1183
                     /locus_tag="ACGE70_002867"
     CDS             476..1183
                     /locus_tag="ACGE70_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694421.1"
                     /translation="MNKVVYQLTTEDVSQFLTSKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGK"
     gene            1348..1548
                     /locus_tag="ACGE70_002868"
     CDS             1348..1548
                     /locus_tag="ACGE70_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694422.1"
                     /translation="MNKIIPLLFVLFVGEILMRHAAAVHLNFVVFLGILVFCLIFWRF
                     VLALVSLLFKMIAAIFFIGLFR"
     gene            1564..1743
                     /locus_tag="ACGE70_002869"
     CDS             1564..1743
                     /locus_tag="ACGE70_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694423.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
BASE COUNT          681 a          291 c          380 g          488 t
ORIGIN      
        1 cgaatattgg ttaaccaaat tagagaacta aaaaggagaa aaaatatggc tcaaaattac
       61 tatgacgaat ttgtcaaatt acctttggat aaaatggcac aaaaaatgga ggatatgacg
      121 ttcctatacc atgaaacacg tgtacccaaa aaacattaca aggaaaaatt gtcagtcgca
      181 gtggaagaaa tgattgaatc aggtgtcgaa atgaatctca ttgcgacgta ctatcgaaca
      241 ctggaagaac tgaaaaaaca aaatggaaaa tggtttttcc aagccttact atgcttggaa
      301 gtaggtgtca aaccaagcac tatcaaacct tccgaatatc aagccttaga actgacttat
      361 gcaaaattcg cacagattaa gaaagctaaa gcaatggcta gcgactggct agatactttt
      421 gaaagtatcg aaaaaaatgg tgcattttat acgattgaat cggaggagaa agataatgaa
      481 taaggtggtt tatcagctaa caacagaaga tgtttcgcaa tttttaacaa gcaaagaaca
      541 cttgaatgaa gtaatcagac ggattcaaaa agactttagt tgtatggaag aaatgaagta
      601 ctttattcaa agagtggaag aacagttgta tatggaaagc gaaacaagtc aaacgaaggt
      661 cacagtcagc cataatcatt tagacgtgta taaacgtcgt cctgaaacgg taaaaaaagg
      721 aacagagctt ctacgactag aaacagaaaa atacatcatg aatgtgtacc tagaaaggag
      781 tgagctggtc gaaaactttg tcagctacac gatttgtagg aaacactatt actcagaatc
      841 agaatattat tcaattttcg agagcatgga attaatggaa gaagaaatgg aagagctaaa
      901 cagcaaaaat gcgacggtag acgattatta ccttgtattt actaggtttt tgacgtgtgg
      961 gacggaatct atcgtgttaa tgggaatgga ttatgaactc gctgtacccg aagcattttt
     1021 gagggaatat gtagaagaag tagataaccc aggcgacgac tggtacacga ctttaactga
     1081 ttttttagac acctacactt acgacgatac agaagaaatc caaaatgaac ttgaccgtaa
     1141 acgaatgcca tatcaacgat ttgaaaggaa aaaaggtaaa taaccattta taccgaaaag
     1201 tgaggatgtg tgaaataaga acaaaaaacc ttgaaattaa gggaatggtt cttatttcgc
     1261 accaatcgct ccaaccgccc ccgccgctta ggtggtgcga aataagcggg caaagcccga
     1321 taacctaaga gaaagaagga gatccatatg aataaaatca ttcctctatt gttcgtactg
     1381 tttgttggcg agatacttat gcgtcatgct gcagctgtac atttaaactt cgtagtattc
     1441 ttaggaatac tggtattttg tttgatcttt tggcgatttg tattagccct tgtgagtcta
     1501 ctattcaaga tgattgcagc catatttttc atagggttat ttcgatgaaa ggagagagta
     1561 ctaatgccaa ttgcaatagg aaataaacgg ttacccgtga cattagatga aaagagacaa
     1621 aaagaattgc agcaactaaa gcagaagtac ggaaaaagtg aatctaagat tatgtgtatt
     1681 gcgttagatc tattgatcgc gcaagaaaaa gcaggatttg atataccagc actaaaaaag
     1741 tgacgtcact tttcatttga agaaacaaaa gtgatagcac ttaattgttc ctactattta
     1801 gaatattttc tatgtccttt ttattccatt ttgtatcaat
//
LOCUS       DAXWOO010000267         1811 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.274, whole genome shotgun sequence.
ACCESSION   DAXWOO010000267 DAXWOO010000000
VERSION     DAXWOO010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.274"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(359..856)
                     /locus_tag="ACGE70_002870"
     CDS             complement(359..856)
                     /locus_tag="ACGE70_002870"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFF8694424.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(896..1705)
                     /locus_tag="ACGE70_002871"
     CDS             complement(896..1705)
                     /locus_tag="ACGE70_002871"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFF8694425.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
BASE COUNT          517 a          338 c          313 g          643 t
ORIGIN      
        1 catcaagtaa taagaacagc caagtaaaaa cagcttctta tgtttttact tggctgttct
       61 tattactgca gatgcaagct tttgaatacc gtttaaggaa gaggttgtga aatcagcgtt
      121 ttgacctgag tattagctta acaaacggaa aaatagttcc tcatattttg ccgtttgtta
      181 agctaatacc gagggtcagc tgattgaaca ccatttaaga aagaggtcgt gagcctgccg
      241 tttcactctg tgtcataaaa aagccaatct actttgatag aaaaagcacg taggactaaa
      301 acagttctgc gtgctttttt tattgtgtgt taaatatcgt aaatgaagat agatattttt
      361 atgattcgac gatagagacg ttgaaatctt tcaacagctc taaagcggat tctccgtgtt
      421 ttctactgga gactaacatt ggatcaacaa aaaagtctgc ttcaggaaaa gcactctgca
      481 ggcctatcag attagagagt acacacaagt ttgtttcaat cccgactact tcgatctctt
      541 tttgttcttc tttgctttca aataaagttt cctggatttc agctaaagtt tcaggtggaa
      601 tcgtaaagta atacttttta atcactcgtt cattttcttg tggagaaaga agagggatca
      661 attgcaaatc ttctcgttct tcttctttgt ccttcagttc aactggtata tcccgtgtga
      721 agagaatata ttcattattt tggcgtgctt tagctaatct ttgagcaata cgttctgcca
      781 attgatctga ctctggaata taaaattcgc tgtttggatc aagaatatga ttttgcatat
      841 caataataac taacattttc tttcctccta cattcatatc gtcattttat tctttttaat
      901 agaagttttt taaaatataa gcaacaccag cttcttcatt cgtcagtgtt acttggtttg
      961 caatttcttt tacttgtcgc tgtgcattcc ccatcgcaat actcaacccg ctgtaattca
     1021 acatagcaat gtcgttttct tgatcgccaa aagccatgat gtcactcgat tcgattccaa
     1081 ggtaatccgc cagaaaaaca agtgaatgag ctttagtgat acctgtaggc gtgatttcta
     1141 agaaatgcgg atcagagaat actgattgga taccatctgc ttgcagatct ctttgtagct
     1201 tctgtagttt ttcttcactc ttatctgtta atgctaattt aagatagcta agagaaggat
     1261 cttccaacat atttaaaatt tgatcaaaag aatggttctg taaattgaaa tgttcataat
     1321 aagggtcatc tttgactaga tttgattcat cgaatgtcag ctgatcgtct tgggtttctc
     1381 ggctagcaat gatggatatt ttcgcttctt gagcatagaa gaaggctttt ctcagtgtat
     1441 cgctatctat ttttgaacgt ctgagaactt tcgccatttt tccatccttc atttcagcaa
     1501 tcagtgaacc attggaagaa atcaaatagc caccgattcc taattcggaa aaaatatctt
     1561 tagcagaaat gctgttcctt ccggtagcga cggttacgat tccgccattt tcatcaaatt
     1621 tccgaagtgc ttggatattt tcttctggta tctttccatt tcgatcaagc aaggtaccgt
     1681 ctaaatcaat cgctgctagt ttcattttat cttcctactt tctttttaca tatctatttt
     1741 cctgttacct taagattaaa tgagaattta aaaaaatgaa actaacttgc tttgtcatta
     1801 tgaatttttt c
//
LOCUS       DAXWOO010000268         1804 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.275, whole genome shotgun sequence.
ACCESSION   DAXWOO010000268 DAXWOO010000000
VERSION     DAXWOO010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.275"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            215..1633
                     /locus_tag="ACGE70_002872"
     CDS             215..1633
                     /locus_tag="ACGE70_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFF8694426.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIDGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
BASE COUNT          568 a          272 c          347 g          617 t
ORIGIN      
        1 actaacttat tttcactagt tagtgaattt ttttgtttgt atatattgtt atgtaaaaaa
       61 agttatcaat cttatgcatc aatgttttta gattattttt tgttttttat ctgtcaagat
      121 aatgcaataa aaagccaaca ttgtgaatat aaaaacaaga aaaaaagagt tataattttg
      181 aaaacgttta ctagaagagg taggaggaat tcaaatgctt tcacaaaatg ctgttacagc
      241 aaatacaata aaaaaagaaa aaaaggaatt tgggatttcc gataagatag gctacatgtt
      301 cggtgatcta ggtaactgtt ttattttagg tttagtcaat agtttcttga tgatttatta
      361 tacgaatgtt cttggcgttt caggggcaat cgtaggtacg ttattcttga tttcgagaat
      421 tttggatgcg tttgctgatg tgatggttgg aagattatgt gatgtctcag agttaacaga
      481 aactggccga tttattcctt ggatcagaaa aatgaagtat ccattttgct tagtgactgt
      541 cattttgttt ttgccattcg tccaacatct tccaatgacg gtgaagattg cttatatttt
      601 tgtttcatac attgtttatg ggatttttct ctcaacaatc aatatcccgt atggagcaat
      661 ggcagcagca attagctcaa atcctaatga ccgcacctcg ttgtccacgt tccgaagcat
      721 tggttctgcg attgacggat caactacagg attcttcatt ccgattttaa tgtataccca
      781 taacaagagt ggacaacaag tcgtttcagg gaatcatttc ttttggattt caatcggatg
      841 tgctgcaata gcatttgtcg catatatgtt aacttgtaaa ttgactactg aaagagtaag
      901 agttgtcaaa aacgaaaaag tatctgcttc tcagttggta aaaggtttgt tagctaatcg
      961 agcactgatt attttagtca ttgttgacat tttcattgtg atcaatcaga ttttagctgg
     1021 gacgaatatg acttatttat tcaatgatta cttccataat aaacaagcaa tgtctgttgc
     1081 tcttttattt acttacggaa cagtagtagt tttagcacca tttgctacaa agctgacaaa
     1141 attatttggt aaaaaggaag caagcgtatt tgcgttactt gcttcttcat gtatgtactt
     1201 gctgatgtat ttccttcata taacaaatgc atgggtatat ctgattcttt tatttatcgc
     1261 tactttagga tctggtttat tcaatttgat ggtctgggca ttcattactg acgtgattga
     1321 ttatcatcaa tataagacag gcttaagaga agatggaaca gtatatggag taaattcttt
     1381 tgcgagaaaa gtaggacaag catgtgcagg agcggtaggc ggatttatgc tggcactcat
     1441 tggttaccaa tcgtctacaa caggtggagc catccaatca gttttagtac aagaaaggat
     1501 ttatacgatg gcaaacttgt taccagcatt ctgcttattt gtatccgcaa tcattttgct
     1561 agtaggttac ccactgaatc gttcaaaaac tgtatttatt ggtgaagaat tgaaaaaaat
     1621 caatgaaaga tagggagaaa cgtattgcgg acaaaaaaag ggattatcaa tgctttgaac
     1681 tttaaaacaa acgacactca acaagatgat acaataatac tgcaaaaagc aatcgatcaa
     1741 ggtgcgaaag aacacttacc tgtatttatt ccaaaaggca tttatttagt cggagccctt
     1801 tttt
//
LOCUS       DAXWOO010000269         1801 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.276, whole genome shotgun sequence.
ACCESSION   DAXWOO010000269 DAXWOO010000000
VERSION     DAXWOO010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.276"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            73..234
                     /locus_tag="ACGE70_002873"
     CDS             73..234
                     /locus_tag="ACGE70_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFF8694427.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            253..1530
                     /locus_tag="ACGE70_002874"
     CDS             253..1530
                     /locus_tag="ACGE70_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8694428.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1540..>1801
                     /locus_tag="ACGE70_002875"
     CDS             1540..>1801
                     /locus_tag="ACGE70_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFF8694429.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQ"
BASE COUNT          740 a          166 c          300 g          595 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcagga taaggataag tgtattaatt gtggaaaatg taaaaaagtt
      121 tgccaaatga ataaagattc agttgagaat tgcaatcatc tggagtgtat cagatgtgga
      181 cgatgtaaaa atgcttgtcc agtggatgca attagttatg gagtaagaaa atagagagga
      241 gtagttagga aagtgataaa aaatgctata gcatatatta caagaaagaa aaatagaact
      301 ttaataattt ttatcatatt aactatagtt ctttcttgct tgtattcttg tttaacaata
      361 atgaaatcaa gcaatgaaat agaaaaggct ttatatgaaa gttctaattc ttcaatatca
      421 ataacaaaaa aagatggtaa atattttaat gttaatcaat ttaaagatat tgaaaaatta
      481 aaagaaattg aagaaaaaat aattcaatat gatggattag caaaactaaa agatgctaaa
      541 gtagttagcg gagaacaaag aataaataga gaagatttat ctgacgaatt taagaatgtt
      601 gtttcactcg aagctacaaa taatactaaa agaaatattt tatttagtag tggagtattt
      661 acaattaaag aaggtaaaaa tatagaagaa aatgataaga attcaattat tgttcatgaa
      721 gaatttgcta aacaaaacaa tctaaaatta ggtgatgaag ttgatcttga attactagat
      781 attgaaaaaa gtggaaaaat aaaaagtcat aaatttaaaa ttatagggat cttttctggt
      841 aaaaaacagg aaacatatac aggattatct tctgatttta gtgaaaatat ggtgtttgta
      901 gattattcaa caagccaaga gatattaaat aattcagaaa ataatgaaat tgcaaataaa
      961 attttaatgt attctggtag tgcagaatct acagacttag ccttaaacaa attgaaagag
     1021 cttaaaattg atgagtcaaa gtattttgtt gaaaaagatt ctaatgcgtt tgaagagtct
     1081 ttagagtcag tgagtggaat aaaatatata ataaaagtaa tgacttattc tattatgtta
     1141 ggtgggatgg ttgttctttc attaatcttg attctatggt taagggaaag aatttatgaa
     1201 ataggtatat tcttatctat tggaacatct aagatacaaa ttataatgca atttatattc
     1261 gagttaatat tcatatcgat accaagtata atatcttcct tatttttagg aaatgtatta
     1321 ctaaaagtaa ttgtagatgg attcattaac tcagaggatt caatgatttc cggtggaagt
     1381 ttaataaata atagtagttt tatgtcaaat ataacaacac ttggacaaag ttatttaata
     1441 ttaataagta ttattgtttt atcagttgta tttgcttctt cattaatgtt aatcaagaag
     1501 cctaaagaaa tcttatcaaa aataagttag gagaaaataa tggatatatt agaaataaag
     1561 aatgtagcat atagttatgc aaattctaaa gaaaaagttt tgtcaggagt aaatcaaaaa
     1621 tttgaacttg ggaagtttta tgcgatagta ggaaagtcag gaacaggaaa atctacactt
     1681 ctttccttac ttgctggact tgataaacct caaacaggaa aaatattgtt taagaatgaa
     1741 gatatacaaa ataaaggata tagtaatcat agaaaaaata atatatcttt agtatttcaa
     1801 a
//
LOCUS       DAXWOO010000270         1765 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.277, whole genome shotgun sequence.
ACCESSION   DAXWOO010000270 DAXWOO010000000
VERSION     DAXWOO010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.277"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(427..>1765)
                     /gene="brnQ"
                     /locus_tag="ACGE70_002876"
     CDS             complement(427..>1765)
                     /gene="brnQ"
                     /locus_tag="ACGE70_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFF8694430.1"
                     /translation="SFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWANLGFL
                     VTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPRLATTS
                     FEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLVLLGAL
                     LLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTIQNMGV
                     TKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIADHYLG
                     TYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLAANVGL
                     TNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDGLNACP
                     ETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD"
BASE COUNT          622 a          358 c          315 g          470 t
ORIGIN      
        1 agaaatgttg tttgatcagc ttctcttcgg tattagttca aaacaaaacg gcaaagtata
       61 tggagctact ttgccgtttt gtttctctaa tactcaggtc agtttataga cgctcaataa
      121 acggtgttca aaagccagtt tctcggtatt aagtcaaatt tatgcaaaaa tgtgagaagc
      181 cattttcaca aaatatttct taatacttga aactaagcgg ctttttcacg acctctcaat
      241 aaacggtgtt caaaagtcag tttctcggta ttaagtcaaa tttatgcaaa aatgtgagaa
      301 gccattttca aaaaatattt cttaatactc gaaactgagc ggctttttca cgacctctca
      361 ataaacagtg ttcaagagac agcctatcgg caataaggct gaacgtctct ttcacaacct
      421 cttttatcaa tctgctactt cttctttctt agcgaaactc caaatcagtc cgataacaaa
      481 accgataact gctggtacga tccaccccat ccccaatttg aaaaacggca agtagctttc
      541 cgcaacatat aaaagatttt gaacaatagg tgtttgtttg atagtttctg ggcaagcgtt
      601 caacccatca aaaatcgaag caatcaatgt aaaataagta gtcattcgat aaacagccgt
      661 acgctgctta aacaaaggac caactaacac tagtaaaatc agtgtcattg ctaatggata
      721 gatgaacatc aaaacaggca acgaaaattg aatgatattc gtcaagccta cgtttgccgc
      781 taaacaagga aggatactag caagtgcaac aaagaatgcg taatttcttt gcggaaacaa
      841 atctgtgaat gtttctgcaa aagctgtaat caaaccaata ccggtcttca agcaagctaa
      901 tataacgata aatgctaata agatactgcc ataagtacct aaataatgat cagcaatctg
      961 agccagcgtg atcccgccat tttcacttag agcaaagcta cctaagctca tagcacctaa
     1021 ataagcgagc aaagtataaa tgattcccat caatataatg ctgatcgcac cagatttgat
     1081 cgtgtcttta gcgatttcag caggttttgt tacacccatg ttttggattg ttgtgacaat
     1141 gatgatccca aatgctaaag cagcaagcgc atctaatgtg ttatatcctt gagtaaaccc
     1201 tgtgaaaaaa gcatgctctt gataatttgg ctgaataggc gcttgattta cagaacctag
     1261 tggattcaaa aaagcaagga gaagcaaagc acctaataaa accaaaaatg caggattcag
     1321 gaactttcca acataatcta atatctttgt cggttttctt gaaaaccacc aagccagcac
     1381 aaaaaataca atactgaaaa ttgctagata tagcgtctgt ctgctaggat cgataaatgg
     1441 tgctaaacca atctcgaaag atgtggttgc aaggcgaggt aaagcaaaaa acggtccgat
     1501 aactaagtaa agcaagatgg taaaaaccat cgcatatgtt tgatttatcc gttttgctaa
     1561 ttcatataca cctgagcttt tcgatacacc aatggcaatc acccctaaga agggaagacc
     1621 gataccggta accaagaaac ctagattcgc ccaaaaggta gcagcgcctg cttcttgacc
     1681 caaatgaacg ggaaagataa gatttccagc accaaaaaat agaccgaaaa gcatggagcc
     1741 tataaaaaga tagtctttaa atgat
//
LOCUS       DAXWOO010000271         1744 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.278, whole genome shotgun sequence.
ACCESSION   DAXWOO010000271 DAXWOO010000000
VERSION     DAXWOO010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.278"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..>1744
                     /locus_tag="ACGE70_002877"
     CDS             <1..>1744
                     /locus_tag="ACGE70_002877"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694431.1"
                     /translation="TQEVDKDATVLSDTPRDLVNLEDTSTDIENVTPQKIGKKDSTSL
                     SEENLSSPASSILRRRTRSLDVQTGETFLEDWALPDNSNISGSLITQTGITYVGVKTD
                     DPTQLHLKMAYRAAATPAAIDWIYIRVDPELTKYIEEINIKNTVNIGAQVGYQRYFEK
                     VDANTLSDVNTFGTPTGVSDAYAQVDQTTGGIYRVLLDKFFTRIPGVKPKERGALSGN
                     WGVFPSVPLEAERYEANIYIKLSKDVAELQQELGRDKFGVQLRLQGQSSSGVATIAKE
                     SLNKNTIIDLSKQESIENNSNWLNSPSVHTITTTYEGKQMGSDGQFSNTLEGTDKSLI
                     RVQNTIMNNLLYGTALDLGRPVSFNIEIDPQILNLVSDDDYITIWQVYRENSINHHDG
                     GSNAPVMLKKNMFVDNKIKIEVNKDKAGQYENGVYYVNAVESFMNTVSSSPFVGANFI
                     DIPVMPKPLIEDEGQLVSAEIKTWFTDKKNDQERVVRNSLASSYINLFPTTINFYDYS
                     TKDTEQLLKLRESTYLVSTFGQEINGMDMGETYEISNYEFVTADPESLIITQKDGNVI
                     NLYYKKLSSDAEFY"
BASE COUNT          626 a          246 c          339 g          533 t
ORIGIN      
        1 atactcaaga agtagataaa gatgcgacag ttttatctga tacaccaaga gatttagtta
       61 atttagagga cactagtaca gatatagaaa atgttactcc acaaaaaata ggtaaaaaag
      121 acagtacgtc tttatcagag gaaaatcttt cgtcaccagc atcaagtatt ttacgcagaa
      181 gaactaggag tttggatgtt caaactggag aaactttttt agaagattgg gcactaccag
      241 acaattctaa catttctgga agtttgatta ctcagactgg aattacttat gtgggtgtaa
      301 aaactgatga tcccactcaa ttacatttga aaatggctta cagagctgca gcaacaccag
      361 ctgcaattga ttggatttat attcgtgtgg atccagagct tacgaaatat attgaagaaa
      421 ttaacataaa aaatactgta aacatcggcg cgcaagttgg ttatcaacga tattttgaaa
      481 aagtagatgc taatacattg agtgatgtaa atactttcgg aaccccgacc ggtgtaagtg
      541 atgcttatgc acaagtagat caaacaactg gcggaatcta tcgtgtgtta ttagataaat
      601 tttttacacg tattccaggt gtaaaaccta aggagcgagg tgcgttatct ggaaactggg
      661 gtgtatttcc atcagttcct ctcgaggctg agagatatga agcaaatatc tatataaaat
      721 tatcaaaaga tgtcgcagag ttacagcaag agcttggaag ggataagttt ggtgttcaac
      781 ttcgtttaca aggtcaatca agttctggtg ttgcaactat tgctaaagaa agtttaaata
      841 aaaatactat tattgatcta tcaaaacagg agtcaattga aaataattca aattggttaa
      901 actcaccatc tgtgcataca attactacta cttatgaagg aaagcaaatg gggagcgatg
      961 gtcagttcag taatactctc gaaggaactg ataaaagtct gattagggtt cagaatacaa
     1021 tcatgaataa tcttttatat ggaacggcac ttgatttagg tcgacctgtt tcatttaata
     1081 ttgagattga tccacaaata ttaaatttag tttcagatga tgattatatt acgatttggc
     1141 aagtatacag agaaaatagt atcaaccatc atgacggtgg atcaaatgca cctgtcatgt
     1201 tgaaaaagaa tatgtttgta gataataaaa ttaaaattga agtcaacaaa gataaagcgg
     1261 gtcagtatga aaacggagtt tactatgtga atgcggttga aagttttatg aatactgtga
     1321 gttcctctcc atttgttggt gcaaacttta ttgatattcc tgttatgcct aaacctttaa
     1381 ttgaagatga aggtcaattg gtatcagcag aaattaaaac atggtttacg gataaaaaaa
     1441 atgatcaaga gagggtagta agaaactcac ttgcttcaag ttatattaat ttattcccta
     1501 ctactattaa tttctatgat tattcaacca aagatacaga acaattattg aagttacgtg
     1561 aatcaaccta tttagtttct acgtttggtc aagaaattaa tggaatggat atgggggaaa
     1621 catacgaaat tagtaattat gagtttgtga ctgccgatcc tgaaagttta ataattacac
     1681 aaaaagatgg aaatgtcatt aatttatatt ataagaaact ttctagtgat gctgaatttt
     1741 atga
//
LOCUS       DAXWOO010000272         1734 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.279, whole genome shotgun sequence.
ACCESSION   DAXWOO010000272 DAXWOO010000000
VERSION     DAXWOO010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.279"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(<1..1087)
                     /locus_tag="ACGE70_002878"
     CDS             complement(<1..1087)
                     /locus_tag="ACGE70_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFF8694432.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKR"
     gene            complement(1197..>1734)
                     /locus_tag="ACGE70_002879"
     CDS             complement(1197..>1734)
                     /locus_tag="ACGE70_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFF8694433.1"
                     /translation="ATVISLVIYFFSIMVSSIGGAFGHSISPGIFGLLSGIIFVYISY
                     NYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVFGYDRAIPQVAS
                     WALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGIWGIISVLVVAS
                     LSMIGVLIGRKVLKKYKK"
BASE COUNT          634 a          326 c          239 g          535 t
ORIGIN      
        1 ttcttttttg ttctaaaact aagttgaaat tttctaaaac acactcttct ttgtatgaaa
       61 agctgacatt ttcaaatgca atgtcacaat ttttaatctg tgtctcgttc ccatatttta
      121 gttggtcttg cttcatttct aaataaaggg agtctagtgt ttcgactgca tatcttgctt
      181 caaacaagtg catacttaca tacattattc tcatgaaaga aacaaaaata attccactta
      241 aaaaaaacaa catcaacaag tcaacagcta atatcttggg agtggtaaga gaagttataa
      301 aaaaggttat aggaactatc aaaatgcaaa ctatgccaaa aaatagccat tgaaaaagaa
      361 caaaaggtgt tttcccactc aaagaatact tatatgcaaa cttagcataa tcgttaattg
      421 ctttattcaa ggctttaaat gatgttactt tagcaccaaa tatctttata acttgaattc
      481 cgcggatata ttctaccgtt tcagagctta acacatctaa cgattcttga tatttttgaa
      541 taaaatttgt attctctgtc attttcttta acaagaatac accaattact aacatggcga
      601 ttagaaccaa tccaatttta aagttaatga aaaagcctaa ggcaactgat aaaacaggtg
      661 ttactatggc ttgagcacta tctggaatca agtgagcaat tgccatatgt gttttagccg
      721 cgttgtcatc aattgtcttt cgaatatatc ccgaagaatt taaatcaaaa aaacgaaaac
      781 ttgcttctgt taaaccgcta attccctttt ttctcaaatt agtctctaag cgaaacccaa
      841 ggatatgaga aaataagcca gaaaaaatat aaaataaagc tcctaaagtt aaccaagcaa
      901 cagtttgaac cgcataactc ttagctaaat attcatctcc tacaataatt agagactgaa
      961 gaaatttgta aatactgtaa tatccataga ctgttaatac gacagaaaaa tcagaaaata
     1021 aagtggctaa ccatccgtag tatttcattt tagggacata actaaataat tttttgtata
     1081 ctttcatttt ttcctccccc gcattcaatt tcttctctta ttcgtttaca actgttaatg
     1141 ctttccgtaa tagcgtaaat tagagatgta caaacagata aaaggtaaac tacgttctat
     1201 tttttgtatt tttttaagac ttttcttcca atcaatacac caatcataga taaacttgct
     1261 acaactaaca ctgaaatgat tccccatata cctaccgcat agtggatcat tgcatccatt
     1321 gttgcagcag attgtccttt atctatccaa tcttgacgaa aacgatctgt aaagaaccat
     1381 ataggaatcc actgtccggc agcactgcct aattgcatta gagcccaaga cgcaacttga
     1441 ggaattgctc gatcatatcc aaatacagat agaataatat cagctaagat cgccatacta
     1501 atcgaactga taaaccaagg taagtacgcc ccgcccatta acgtaaaaaa taacaataaa
     1561 acaattgtgt aaatagtaaa aattcctttt ctagggtagt tatagctaat ataaacaaat
     1621 atgattccag ataataatcc aaaaatcccg ggggatatgc tgtgaccaaa cgccccacca
     1681 atactactaa ccattattga aaaaaagtaa attactaggg aaataactgt agct
//
LOCUS       DAXWOO010000273         1729 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.280, whole genome shotgun sequence.
ACCESSION   DAXWOO010000273 DAXWOO010000000
VERSION     DAXWOO010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.280"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            136..309
                     /locus_tag="ACGE70_002880"
     CDS             136..309
                     /locus_tag="ACGE70_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694434.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
     gene            949..1422
                     /locus_tag="ACGE70_002881"
     CDS             949..1422
                     /locus_tag="ACGE70_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFF8694435.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1431..1658
                     /locus_tag="ACGE70_002882"
     CDS             1431..1658
                     /locus_tag="ACGE70_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8694436.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          582 a          269 c          258 g          620 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttcttacaat caaaaaaagc tgttaaatgc actccacacc ttcatactct
      841 atcatttttt ataaacgata tatttttatt gtttttcgat aaaaaatagt tgttttacaa
      901 taattattct gctatatttt agctatcaaa gataaaagag ggataaaaat gaaatttaaa
      961 acattgctat taaaatttgt cgttttaggt ataagtgccg tatttatttt gttcggcgta
     1021 cttttttata ctcagatcgc tacaagcgaa aaaggattca attttgattg gcagaccaca
     1081 ttatttgttt ctgtcattta ctttgccctt ctcctaggga tcgttattgc ttatttctta
     1141 ttgaaactat tgaaaaaaat cgaagataac caagcttttt ccactcaaag cttaaacata
     1201 ctaaaaaaaa tcgaacgaac cattttgttt attggtttcg ccttttttgg cttacttcct
     1261 aaatttatga aagtagcgga tttagacgat gctccaggtt tgatgttggt aggaatcgct
     1321 gtcgtattca ttccttttgc agtatacaca ttaacaagcg tgctggaaaa actgcttgaa
     1381 catgcgattt tacttaagac agatcatgat ttgacagtgt gaggtgcgac atggcaatta
     1441 tcgttaattt agatgtagta ttagctcaaa aaaaaatgag tgtgactgaa ttgagtgaga
     1501 aagtgggcat tacgatggca aacatctcta ttctaaaaaa tggaaaagca aaagccatcc
     1561 gtttttccac tctcgaaaaa atctgccaag ttttggagtg tcaaccaggt gatttgttga
     1621 cctataaaaa tgactcgaat actataccag aagaataaaa taaagtagaa caaacagtaa
     1681 ataaaaaata atctgaatga tagatgtgtg agacttttag aagttattt
//
LOCUS       DAXWOO010000274         1655 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.281, whole genome shotgun sequence.
ACCESSION   DAXWOO010000274 DAXWOO010000000
VERSION     DAXWOO010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.281"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(1..334)
                     /locus_tag="ACGE70_002883"
                     /pseudo
     CDS             complement(1..334)
                     /locus_tag="ACGE70_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            609..1151
                     /locus_tag="ACGE70_002884"
     CDS             609..1151
                     /locus_tag="ACGE70_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694437.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            1307..>1655
                     /locus_tag="ACGE70_002885"
     CDS             1307..>1655
                     /locus_tag="ACGE70_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFF8694438.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          603 a          220 c          315 g          517 t
ORIGIN      
        1 ttgcacatcg ctgatatgga atatttatgc ttgttggcat caattacttg tcttttcgtc
       61 cgaatatcag cgccgcttgc tttaaaatat cattctccat ttcgagttgc ttatttttct
      121 ttcttagtgc gatcaattct gcttgttctg gtgttaagtt gtctctttct ttgaatgagc
      181 ccgttgattg tgcttgcttc atccatttat cgaaagaaga aggcgtaagc tcatactctc
      241 gaataatttc tgcgcgaggc ttaccagcga gatagagatc gacgatttgt tgtttgaatt
      301 cttttgaata ggttcttcgt tgacgtcttg acatgaaaaa tcctccagtg tgttttttat
      361 tattctacac accttatttt ttctgtctag tctagtgtag ccgattcaaa ttgatgaagc
      421 agatcccaaa gggctgcttc atcaattttt tcgtaagtgt ccgtacactt actgtgcttg
      481 ctcatatatt aatcgatcat atactaatcg tgtaagaaca actttaagca taatacttat
      541 attgttatac catagtttgg aaaaataaaa aattatattt catcaacgtt tcataaaagg
      601 tgtgtagaat gttattgaaa gtagctataa aatgctattg tgtaaaaaat aaaggaggaa
      661 taatttatga aaaaatagta ccaagaggag catgcctatt acttgcatta tgtttgagct
      721 taagcgcagt tgggtgtcaa aaaagtaatg aaaacaataa aataaaagaa ggacaatcca
      781 taagttctga agaggaaggc atgtcaatta atgcaaatgg caaaaatgaa acttttaagc
      841 catcagatta tacattggag gcaaagaaag aatatgttta tgaatattta gggcttaaat
      901 ttaaactctc agacaaattt agaaattata tagctgataa aaagatagcg atgttagatg
      961 atcaaagtcc tatagataaa gaactaaagt atgctatttt gacatttgag aaaatgacag
     1021 aagaacaaaa gaatgcagtt atagaaaaga tgggcgatgg atataaaaat tggcaaaatg
     1081 agcttgaaag aattgggact attggtatat ttgaaaaaaa tacatcagaa gaaaaaaatc
     1141 taaaattata aaatgtgata ctcatactaa aataggagtt tcaagtgatg gaaaatacga
     1201 ttgctattta agcacaaata gcggagctga aagtaatctt ctggatgaat taaaaagaac
     1261 tgagattcaa attatagata aaaaagaacg tcctaaaaat ggttttgtgc tatcagaaaa
     1321 aactgattta gaaaatacag aagcatttaa caaagaatcg gttaaagatt tacgtaaact
     1381 accaacaaag gacataaatg gcaaggattt tacaagtaaa gattttgaga agtatgatct
     1441 tactatggta aatgtatttg caacttggtg tacagcttgt gtaaaggaaa ttcctgattt
     1501 ggttgaggtt cagaatgaaa tgaaaagcaa gggtgtaaat atcgttggag ttgttacaga
     1561 tcctgttgat gataacggtg aaaacaagga agcaattgaa aagtcaaagt taatacatga
     1621 aaagacaaag gcttcatatc ctttcttaat gccag
//
LOCUS       DAXWOO010000275         1652 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.282, whole genome shotgun sequence.
ACCESSION   DAXWOO010000275 DAXWOO010000000
VERSION     DAXWOO010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.282"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(<1..834)
                     /locus_tag="ACGE70_002886"
     CDS             complement(<1..834)
                     /locus_tag="ACGE70_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HFF8694439.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SS"
     gene            complement(993..1259)
                     /locus_tag="ACGE70_002887"
     CDS             complement(993..1259)
                     /locus_tag="ACGE70_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFF8694440.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(1249..1605)
                     /locus_tag="ACGE70_002888"
     CDS             complement(1249..1605)
                     /locus_tag="ACGE70_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFF8694441.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          470 a          318 c          199 g          665 t
ORIGIN      
        1 agaagaatcg tattcttcct ttaaaattcc cgatttactt aatttttcta gtacatcaaa
       61 tactctatca ctcaagtaac tccttaaatt gttatcactt tcccattgaa ctacttttct
      121 ataagttgaa ttatcagttg aaaagataga attttcatcc ataaacgcat atattagcag
      181 caatctttca tcattgtcaa attctattgg atcaaaaata tctaagttgt catcaaaatc
      241 tttatcagtt ccggtaactt ttttgttaat tactgaaggt gtcttgtaag aaatagaatc
      301 taaatcaaag cgttttgatt ctaaattatt ttgtgctttc tcaattaaat aattcacttt
      361 ttttgtgaga aaccttctga tattgtcttt gctatatgtt ccataaaaac ccttatcttc
      421 taaagagtcc ctgtacttct taacttctat atactcttca gccaaaactt gtggaactgg
      481 ttcatctaag aaaaaaaccc ctacctcgcc tttgctattt ttataaaagt aatcaatttc
      541 tgctatagtt cccgatctat aattatcaac tggagaaccc aacttatggc caaaaatcgc
      601 taataatata tcactatttt ctacaatttg tttattaata acactttgcc cagagctatt
      661 atttatataa gtaggaatcg cattcgtttc ccatcgaatt ggcatcaaaa ttacttttgt
      721 attatctgta ttttctatat tccatctata tatggtttct tctacttcat tcctctttgc
      781 tgctatatca cctggtgatg ctataaatac ttttattaga tttatggttt tcatttattg
      841 tacctttctc tttatgtctc tttaaagtaa tttttgtata aaatttattg acttttacac
      901 tataggacaa cttttatcta tttagtacaa aaaggcccac taaactaatt gattagttag
      961 gagcctatta caggtatatt ttcaactcgc ttttaagcaa attctactac cagctttttg
     1021 cctaatgccc tagcgatatc atcaagttta tcgaaagata cgttatcccc tctctcaatt
     1081 cgagcaatag tagattgagt tgtatgtgct ttttgtgcaa gctccgcttg agttaggcct
     1141 gcttcctccc gtgcatgata aagagcaacc gcagtttcga gttttcttcc ttcacggtca
     1201 tatgcttctg caaaatcttt gtctttttgc ttttcttcaa tcaatttttt aatcatcatt
     1261 tgtgaaccac tcctctctaa gttcttttgc atgtttaagt tcacttattg gtgttttctc
     1321 cgttttttta gtaaatccat gggtaatgac ataccttcca ttgaccacat ggaaatagat
     1381 agctctttgg atgtttgatc ctaccttact tcttaattcg tacaaattag tgtctaattt
     1441 tttcacccat ttcaaccgtt gagctactag catcccattc ttttctgttt cttctattgt
     1501 acgtaaaagc ttcgcactat ctttttttgg taatgtttcc aaccattcta aaaattcatc
     1561 atgaccatta ggtcttttaa atgtttcaaa agatattttt tccataccca attatatcat
     1621 atattgattt tatattatag tatataacat aa
//
LOCUS       DAXWOO010000276         1652 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.283, whole genome shotgun sequence.
ACCESSION   DAXWOO010000276 DAXWOO010000000
VERSION     DAXWOO010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.283"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            54..1094
                     /locus_tag="ACGE70_002889"
     CDS             54..1094
                     /locus_tag="ACGE70_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFF8694442.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
BASE COUNT          635 a          241 c          279 g          497 t
ORIGIN      
        1 tcattgtaac atagttttga acaaaacgct atcctacaga aagaggattg gctatgaatg
       61 attttaatta ttacaaatca aaagaaattt atcgtgaaaa atattatcaa atgcctaagg
      121 tattttttac taacgaaaaa tacatggatc tatcaaatga tgcaaaaatt gcttatatgc
      181 tacttaaaga tcgatttgat tattcagtca aaaataactg ggtagattct gatgataata
      241 tatttttcat attcacagtt gaagaattaa tgaaattgtt acaatgccgc gaaggtaaag
      301 tatctaaaat caaaaaggaa ttagaaaaag caggtcttct gaaacagaag agaggaagag
      361 taaataaacg tgacggtaag attgaatcca tgcctaattt attgtactta ggcaagccag
      421 aagtaacgaa tcaagacgtc tttaaaatca tggaagagga agacaatact gataccaccg
      481 taattgcgaa aatcgcaaat acggcaaaac cgcgagaaat caatgatacc accgtaattg
      541 cgaaaatcgc aaatacggca aaatccagtc atatcaagga ctcaaccgta attgcgaaaa
      601 tcgcagacaa tctattctat tctaattcta tagacactaa tagacacata atagacactg
      661 aaactgatca gttacaaaat caagttttac tagataactt cgtcgacatc atgaaagagg
      721 acagtataaa tacatttgta ccagaaaatg tattaaactt gataaaaaca ttttctagta
      781 cttacgaaga cgctcaaaaa accgtccaaa ctattcataa tgcaaagaaa aaagctgaag
      841 aattagagca aacagttgtt gtttatgaag aactggaatc ttatggaatt gatgctgaca
      901 aaggattata tatgactttg ctgaaagctt atcaaaaaca aaaaactgaa aaagtaaaca
      961 atttgcagaa tctaattttc gtttatgtaa aaaattggtt tgtcgaaaaa gctattgcgg
     1021 caaagcttgc aaacgaacaa agagaaacat ttaacgaatt accaacagtt agtacggaaa
     1081 attggctaga gtagttgata aaaagcgatc actataaaaa tgatcaattt taaattagaa
     1141 cttttttatg attttaattt tattacaaat gttacaaata aatagctatt attccggagt
     1201 ttttagatta aaagtttatg aaaacaatca caaacgtagg ttttaaagtc ataatcgtta
     1261 gatattttag ttatttttca ttcaaaaaaa tagggaacta gtgtttgcat atgtgctttt
     1321 ttgagttata ataattcatg aagagaggta attcacttat ttccccaagc tgaaaagcct
     1381 gatattactt cttttcaggc agtgctgcta atattcaatt acactttttt gtatattttt
     1441 agttgtttta aatttaattg tctgatatgc ttgtgatgaa ttgattttcg ttctgcactt
     1501 ttggacgata aattgtgata attgaaaata caaaaaaaga aaggcccctg taaggctcgg
     1561 gagactggta atctccgtca gtgtcctaca ggaaagcatt aagatgacgc cttaatgcgt
     1621 gcccttcagt gttacggtat tacccgcaac gg
//
LOCUS       DAXWOO010000277         1650 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.284, whole genome shotgun sequence.
ACCESSION   DAXWOO010000277 DAXWOO010000000
VERSION     DAXWOO010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.284"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..302
                     /locus_tag="ACGE70_002890"
     CDS             <1..302
                     /locus_tag="ACGE70_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFF8694443.1"
                     /translation="ITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQYLEFLLVP
                     VLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFLSILI"
     gene            313..>1650
                     /locus_tag="ACGE70_002891"
     CDS             313..>1650
                     /locus_tag="ACGE70_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8694444.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNG"
BASE COUNT          609 a          208 c          287 g          546 t
ORIGIN      
        1 gtattacagt agtgatcaga ttctttccga cttatcgttc tgaaataaga atgattaaag
       61 aatctttaaa aatgagaaac attcgcttgt ctttttttca gcctctgcaa tatttggaat
      121 ttctgttagt tcccgttctt atacgtgcaa ctatgattgc tgaagaaatg actgctaatg
      181 caataacaaa ggggatagaa tctcctgtga aaagaagttc ttactataaa acaaacatga
      241 gcttattaga ctatatcatc ctgttttccg ttactatttt tttcttaagt attttgattt
      301 gaggtgaaag aattgtttga atttaacaat gtttcttttt catataagga tgttaaggaa
      361 caagcgcttg ttgaccttaa tttttcaata aaaaaaggca gtcttacatt gattactggt
      421 tcttctggga gtgggaaatc cacactatta aagttaatga atggacttat tccaagactt
      481 tatgacggaa aattggttgg ggaaatttta tttgaaaata actcgctaca atccttaaca
      541 caagaaagta tatcaaaaaa tattggctac gtaagtcaag atcctagagg acaattcttt
      601 acaacaaata caacgagtga gttagtattt tcgatggaaa actttggatt tacaaaagag
      661 catatggata aaagaatcaa tgaattagct gaattattag atctttcaaa tatattagat
      721 aaaaacattt tttcgctatc aagtggtgaa cggcagaaaa tttctattgg atgtagttta
      781 tcgctaaact ctaaattaat tatattggat gaaccgtctt caaatttgga cttcaaagcg
      841 actaagaaac ttgctgactt actttgtgat ttaaaaaata aaggatttac tattgttata
      901 gctgaacatc gtttttacta tttgaaagaa ttaattgatc aagttctatt tgttaaaaat
      961 agaactgtaa aaactacaac tattcagttg ttaaaaactg aaaacaataa cgcattaagg
     1021 aaatttgaca tttttaatag ttcgattgaa gttccctata tcgaaaaggt aagtgcagat
     1081 gtattaaaag ttgaagaaat tacttatgaa aatattctaa aagacataaa ttttgatgtc
     1141 aaagttggag atgttattgg tttggtaggg aaaaacggtg tggggaaaac aaccttacta
     1201 agaatactca tgaagattat tcgccctacc aaaggaaaga taatcgaaaa taaaaaaaat
     1261 ttgtcatcgc cgtttttagt aatgcaagaa atggattacc aatttttcac agaatcagtg
     1321 aggagcgagt tagccctggg gaatgaaata gttagtaaag atcagcaaga aaaaatttta
     1381 aaaaaaatgg aactttataa aatgaaagat caattaccgt ttgatttatc cggtggagaa
     1441 aaacaaagat tattggtttc tatagcttca ttgtcaaaaa ctaatttatt tctatttgat
     1501 gaacctacaa gtgggttgga ttatattaat atggaaagga ttgctgacct tattaaagac
     1561 ttaaaagaaa agggtgcagt agttatagct actcatgatc cggaatttct ttataaaaca
     1621 tgcaatagaa ttatttattt aaaaaatggt
//
LOCUS       DAXWOO010000278         1642 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.285, whole genome shotgun sequence.
ACCESSION   DAXWOO010000278 DAXWOO010000000
VERSION     DAXWOO010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.285"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(128..1213)
                     /gene="xerS"
                     /locus_tag="ACGE70_002892"
     CDS             complement(128..1213)
                     /gene="xerS"
                     /locus_tag="ACGE70_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFF8694445.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
     gene            1562..1642
                     /locus_tag="ACGE70_002893"
                     /pseudo
     CDS             1562..1642
                     /locus_tag="ACGE70_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
BASE COUNT          518 a          221 c          279 g          624 t
ORIGIN      
        1 tcaaaaaaac ttctaatggt gtttggtaat ttagtaatat aaaaatgata atgaagcttg
       61 cctttaaagt tgggctttta tctaatttta gagacaggct tcattattat actatttttt
      121 actacattta caaagcgttc aaagcatcaa ttgtagctgc attgtctata tgagtatata
      181 aagttgttgt atttgtactt gtatgtccca attgttgggc agttaatacc aatgaatttg
      241 tctgttgata gagtctagta gccaaagtat gacgtaattt atgtggactg acttggactc
      301 tgaatccttt tgaatatttt gctaccatcc gttcgattgc aataccggat atacgcttgg
      361 cttctccttg ataaattgct agaaataaag ctttttcgtt ttctgttact ttgtatcgtt
      421 ctttccgaat agctagataa ttagctaaat attccattgc aaatgctgct atatttactg
      481 cgtctctctt atttcctttt ctagttacta ccacattatt cttttctaaa gataggtcct
      541 ctaaattaat atttacaacc tcggagagtc ttaatccaga tcctaaaatc aatgcattga
      601 tcgccaggtc tctttctaca tctcgttgat aatagatcaa agctcgtttg ctcaaagttt
      661 ttttatattc attggcaata taatctagat aattttgtgt atcttcgtct agaaataatt
      721 ttttctcgat tgcgtttgca cgggcagcta acgtagtttt atctgtgata gttgctactt
      781 gatccatagg attattttct aagtatggtt ttcctaaagg attattggaa cttttcgaaa
      841 gatatttaaa tagaaccttt aatgctgtga tatttctatt gatagtagaa gtagatagac
      901 ctaagttatt attatctttg agcttagttt ttgttgtctc tttttttaca ttttcctttg
      961 tgcgacgttt caaatgagat ttgtacaagt ttagatcata ggcagttaac tgtcttatcg
     1021 aggtgattga agtatcggga acgatagact cactcagaag ccattcataa aagagcttat
     1081 aattttttag atattctaaa gtagttcgag gtgattgatc tttatcctca cgatcttgaa
     1141 caaaatcttt tatataatcg ggcatatgaa gtatgattgt atctattttt tgatatattt
     1201 ctgtattttt catgttattc atcctttcga taagtagggg agagaagtat ctctataatt
     1261 ggactatacc aattatagaa tgttttgtca attggtatac tctgaaattt attaatttat
     1321 ttcaaaaaag cagataaata ggtatagatt taataattta aaataaatgg tatacatgaa
     1381 taggaaagga aacagaagga ggcaatctta attttttaaa ttgtctaaat tatatagaaa
     1441 gaacaatatt tctatataat tagacataag agagactacg gttgatttga tattaacaag
     1501 cgtatatgaa tgtatataca aataaaaaag gggagggaga agttaaagaa aattcagaga
     1561 agtggggcac tcagtagtat taacaattta aaaggaattt cttaaagaaa atgggttgca
     1621 tcctggagat acaatttttt ta
//
LOCUS       DAXWOO010000279         1603 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.286, whole genome shotgun sequence.
ACCESSION   DAXWOO010000279 DAXWOO010000000
VERSION     DAXWOO010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.286"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            92..1558
                     /locus_tag="ACGE70_002894"
     CDS             92..1558
                     /locus_tag="ACGE70_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFF8694446.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          446 a          292 c          334 g          531 t
ORIGIN      
        1 agtttatatt tcttaaataa gttttttagt taaatttttc ggtaaagatg tataatattt
       61 tctgaaaatt taaagaattg gggtatagat tatggaaaaa cagcaagaca agggcttctt
      121 cgggcagccg aagggattgt caactttgtt cttcactgag atgtgggaac gatttagtta
      181 ttacggtatg cgtgcattac ttatctatta tatgtacgat acagttgcca acgggggact
      241 tggattacca cgagcaacag cattagctat catgtcgatc tacggatctt tgatctacat
      301 gtctagtatc gtcggcggat gggtatctga ccgagtgtta ggagcaaaac gcactgtatt
      361 cttcggtgga gttttcatta tgcttggcca catcgtcttg gctttaccat ttggtgtaag
      421 tacattgttt gtttcgatgg cattgattat tatcggaacc gcctttttga aaccaaatgt
      481 ttctggcatg gtcggacatc tttattcaaa gactgacttg cgaagagatg caggattttc
      541 gattttttac atgggtgtca acttaggggc attattagca ccactaatcg ttggtacaat
      601 cggtcaagaa tataactatc atttagggtt ctctttagct gctattggga tgttctttgg
      661 attattacaa tacatcatcc aaggacgcaa gacattagac ggagtgggta caacaccgcc
      721 gaatcctttg acaactgaag aacgtaaagg attcatccgt aacttgatca tcgcacttgt
      781 tgttatcgct gcagtattcg gcggggcaca actaactggt catctgacaa ttgacttttt
      841 tgtcaataca atcagtgtat tggggattat cttaccgttg tattacttta tcaaaatgct
      901 gacttcaaaa gatgtaactg ctgaagaaaa accaagagta tgggcttata tcccattatt
      961 ccttgcagca atcattttct ggtcattaca agaacaaggt tcatctatct tagctttatt
     1021 tgctagtgaa cgtacacaat ctacgctatt tggtgtaacg atccaagcaa gctggtatca
     1081 atcattgaat ccaattttca tctttattct gacaccagta ttcgtaacga tgtggacgaa
     1141 acttggtgaa cgtcaaccat ctacagtagt gaaattctct cttggacttt tatttgcagg
     1201 attatcgtac ttgctattga tgttgccggg tatgctttat ggtacaagtg gaaaagtaag
     1261 tccattgtgg ttgttgggaa gtttcttcat cgtggttatt gctgaattgt gtctttcacc
     1321 agttggtttg tctgtgacaa caaaattagc accacgagca tttgaatcac aaacaatcgc
     1381 tatttggttc ttagctgatg cttcttcaca agcaatcaat gcacaaattg cacgcttcta
     1441 cacaccggga acagaatctg cttacttcgg tattatcggt gcagtagcaa tcgtcggtgg
     1501 tatcttgcta tttactgtga aagaaccaat caaaaaacta atgggctcta ttcattaatc
     1561 aatgaacata aaaacaggaa ttgcttattt gcagcagttc ctg
//
LOCUS       DAXWOO010000280         1602 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.287, whole genome shotgun sequence.
ACCESSION   DAXWOO010000280 DAXWOO010000000
VERSION     DAXWOO010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.287"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..1542
                     /locus_tag="ACGE70_002895"
     CDS             <1..1542
                     /locus_tag="ACGE70_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8694447.1"
                     /translation="EAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHMSESN
                     FLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESVSYQI
                     LTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSFSGKE
                     VQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKLYFLL
                     KICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILARLSI
                     TKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAFARNK
                     LYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILEKENV
                     QWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQNDFMEN
                     CNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQSKKE
                     NQVTS"
BASE COUNT          575 a          260 c          250 g          517 t
ORIGIN      
        1 gaagcgatta aaacgtttct cttgaagaaa aaaagcaata caaaattaga tatttttctt
       61 ttcctatctg aacatcgttt ctttattact acacaatatt tagcagatca tttccatatg
      121 tctgaatcaa attttttact atacatcaaa gaattagaac aagactttga gcgtctcaat
      181 ctaacagaac tgcatattga taagcaaaaa ccttttttaa agttgaattt tgaaggaatt
      241 gatccggctt actgctatta tcgtctattt ggaagatact gcaacgaatc agtcagttat
      301 cagatcttga cttctttgtt cagctgtcag acaaattcga ttatcagttt aagtcagcaa
      361 acaaactata gtgcctctta tttgtatacg aaaatgaaaa agattaatgc atttttagca
      421 ttgtacggtt tgtctatctc cttttctaat aatggcagaa agtctttttc tggaaaagag
      481 gtacaaattc aatatgcagc attagatatt tattggaaca tcttttcaag tactgctaca
      541 gaattttatg atgaagatcc gcaagtagtg gcttttatga tgaatacttt ttttaaaaaa
      601 gaagtacttg accgactaaa tagcggtatg atagataaat tatattttct attaaaaata
      661 tgtaaagaga attttcctgc aacttctgtg aaagacgtac aaaaagaatt cacagagtat
      721 gaatatattg acttttttat tgatccctct gtagatattt tgcgacctaa tctgccaatt
      781 tgtcgcgaac aacgcatctt agtgaatatt ttagctcgac tttctatcac gaaaatcgaa
      841 tcggaagaaa caagcttgga gcaatacaaa cttttaaaaa aagcaaacgt tcctcatgtt
      901 ctttattcaa gagaaatggt ggagtccttt tgtactgtat ttgatttgtt tatacctgaa
      961 aaagaaaaga ttctttactc attggcattc gcacgaaata aactttataa tggatttctt
     1021 aacttcaatc aaccaaatac gccccttcct actttcatgc tttatcaaga acatagcgtg
     1081 ttgaaagaag tccaaaccgc cattgaacaa ttttatatgg atttcagaga acaaaatcaa
     1141 cacttgttac cagttatttt agaaaaggaa aatgtgcaat ggatggttga agacctagta
     1201 catttgtatg atcgctacaa aaaacagccg cgaattgtga ttggtgtgaa ctatacgcgt
     1261 gatttttata tcagtaaaga tctgatcgtt aagatagaac aaattttttc aaatgaatcg
     1321 atcaaaatac aaaatgattt tatggagaac tgcaacatcg ttatcagcga ctgccctttg
     1381 aaacaattgc cttctcatat caaaaaaatc tatattttaa aaggaatcat cacaccagaa
     1441 gactggaaaa aagtgattat gaggatctca gaatatatct ttgaattaaa agaagaagca
     1501 ctcatggatc aatcgaaaaa agaaaatcaa gttacttcct agaccaaata agatacataa
     1561 atccgaatgc ttattaacaa cctccggatt tatgtatcaa ga
//
LOCUS       DAXWOO010000281         1590 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.288, whole genome shotgun sequence.
ACCESSION   DAXWOO010000281 DAXWOO010000000
VERSION     DAXWOO010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.288"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(451..>1590)
                     /locus_tag="ACGE70_002896"
     CDS             complement(451..>1590)
                     /locus_tag="ACGE70_002896"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFF8694448.1"
                     /translation="SHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSA
                     KDILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYH
                     AEHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYEC
                     DLACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIH
                     DIDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDL
                     AQMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIED
                     VCFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
BASE COUNT          447 a          334 c          248 g          561 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact
//
LOCUS       DAXWOO010000282         1588 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.289, whole genome shotgun sequence.
ACCESSION   DAXWOO010000282 DAXWOO010000000
VERSION     DAXWOO010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.289"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            196..810
                     /locus_tag="ACGE70_002897"
     CDS             196..810
                     /locus_tag="ACGE70_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFF8694449.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRL
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            complement(834..1184)
                     /locus_tag="ACGE70_002898"
     CDS             complement(834..1184)
                     /locus_tag="ACGE70_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFF8694450.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(1178..1441)
                     /locus_tag="ACGE70_002899"
     CDS             complement(1178..1441)
                     /locus_tag="ACGE70_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HFF8694451.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
BASE COUNT          519 a          288 c          245 g          536 t
ORIGIN      
        1 ttttcttgat tgacatttat ttacaaaaaa tattgccatt taaaaagaaa agtgacacat
       61 actatttcct ccatcattag tttttgtcta tataaggtat accctaacta gacacgaccg
      121 cgacattgaa aaatctggtt ttctcgtgta tgattagggt gttttttaaa tagttgcaga
      181 aagtaggtaa aaattttgag agttgcatac atacgtgtct catccatcga tcaaaatgaa
      241 caacggcaga ttgaagaaat gaaaaaattc ggtgcagaga gaattttcat tgagaaacaa
      301 tctggcgcaa cgattactca tcgacttgtt tttcaagaag cactggattt cgtccgagat
      361 caagatattt ttattgtgga agctattgat cgacttggaa gaaattacga cgagatcatc
      421 gcttcagtca attacttgaa gaagaaaaat gtgcagctga tcatcacaag tctaccgatc
      481 atggccgaag caattggcaa tcctctcctg gataaattca ttaaagattt gatcattcaa
      541 attttggcca tgatcgctga acaagaacgt accgaatcta aacgacgtca ggcacaaggc
      601 attaagattg caaaagcgaa cggtgtttat aaaggacgtc ctaagctata cagtgccaat
      661 gcaaaagatc cccaacgtcg tcttgtttat aaaaacatcg ttgaggatct aaaaaaaggt
      721 gttgcgatcg ctaagattgc aaaagactac aatgttacga gacaaaccgt atatagaata
      781 aaaaaagaca gcatggtcaa tcacgaatag ttgatcatgc tgctgtttct attttatttg
      841 cttacttttt gaaaaactgt tctaactttc atcaataact cctcaactaa actatcatgc
      901 gctttttcca aaaaaataca ctctctggct tcataatcaa ttgtcgttag ttgatctaat
      961 aatacttttc ctgttgtctt catttcatat tctgagttta tatttacata tagcggatag
     1021 tttctagttg tactagaaat tggagcaact gtaacaacgt tactataatc cgcaataata
     1081 tcatgactta aaacaatata aggtctttta cctgtctgtt cgtgtccaga tcgaggtgct
     1141 gtattcaata atagaatatc gccttgtcta ggttttctca ccatttttca ttacctactg
     1201 cttcacctaa attttgaatc gtcacatgat ttctttcatc agtgtaattt tcaaataact
     1261 gttcaattgt taattcattt tctatattag ccgcttcgat aattagccgc ttctgaccgt
     1321 ttacttcttc ttgtattatc ttcactttgt cttctgtatg aattccaaga tattccataa
     1381 tatactttgg caatctaagg ccattactat ttccccattt ttttataggt aacatttcca
     1441 tagtagacac ctctttctgt atatacatag tataacatac ataagaaaaa acgtatatac
     1501 ttaattatcc ttttctatta aattagatta acattccatg aatccataaa ctatgttaac
     1561 atttatatga aaatagtcga aaggaaat
//
LOCUS       DAXWOO010000283         1564 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.290, whole genome shotgun sequence.
ACCESSION   DAXWOO010000283 DAXWOO010000000
VERSION     DAXWOO010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.290"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(1..378)
                     /locus_tag="ACGE70_002900"
     CDS             complement(1..378)
                     /locus_tag="ACGE70_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694452.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(678..1559)
                     /gene="galU"
                     /locus_tag="ACGE70_002901"
     CDS             complement(678..1559)
                     /gene="galU"
                     /locus_tag="ACGE70_002901"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFF8694453.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          428 a          352 c          239 g          545 t
ORIGIN      
        1 ttaccgtctt cttctcgttc ttctactttg ttcgagagaa tctgtactag aaaaagtagt
       61 tgtatgtcta ccttttctgc ctatccgaga ctttcttttc atatatggga tataaatgat
      121 tagatacagt acagcaaaaa ataaagcaaa tacgcaagct tcccaactac ctaacgccaa
      181 ttcaaccatc aaggtagcaa ccccaacgga tgctgcatac cagataaaaa tcagaaacgt
      241 ttcttttttt ctgaccatgc gaaacatgat aaatagacta ataagcaata gtggcaacac
      301 aaatatccaa ttgcttcctt ccatcagcca cttgagtata tcaagcagcg actgtgcaac
      361 agcgactaat tttcccatag tgaacacaca ccttttttta taattaaaaa attcgctttt
      421 ttattctact atgaatcttc tggcttggct ttcatttctc ttatgtaatc attttctttt
      481 atacaactta aaaaaaaatt aaaccattag cataatattt tctgaatcta ttcacgaaag
      541 tctttttcta tctttcgttt tccacgactc aactgatcta gagtcaaaaa actaaaacga
      601 cagttgcgca aatgacgcac aagctgccgc tttaataaca aatagccttt cattctcagc
      661 aggccactta tttccagtta actttctgtt cttagctttt tactcaactg gataatatat
      721 gcacttaatg aagtcccaat ttccgaatgt tgaagtccat attcaatatt tgtcttcata
      781 tatcctaact tatcgcctac gtcgtatctt tttcctttaa actctctcgc atagaccgcc
      841 tctgtctggt taagagtctg aagtgcatcc gttagctgta tctcgtttcc tgctcctggt
      901 tcttgagttt ctaatatctc aaatatttta ggagtcagta gataacgtcc gataatggct
      961 aaattacttg gtgcttttcc catttcaggt ttttctacaa acccttttac tctgcaacga
     1021 cccttttcgc tatctgtttt aggatcaata attccgtatt tagatgtttc attttctggt
     1081 acacgcatga cggcaacagc ggaagcttgt gtttcttcat aatccatcat caattgttga
     1141 gtcaatggga tatcatcttt catcaaatca tcccccagca tgacgacaaa cggttcattt
     1201 cctataaatg cttttgcttg aagcacggca tcccccagtc ctcttggatg agattgacgg
     1261 atgaagtgaa gattgacttg agtcgtttct tcaactaaag cgagtaattc tgttttttcc
     1321 ttttcccgca aattgttttc caattcgata tttgcatcaa agtgatcttc aatgggtctt
     1381 ttttcttttc ccgttacgat caagatgtct tcgattcccg aatccaatgc ttcttccaca
     1441 ataaactgga tcgttggttt atccacgatt ggaagcattt cttttgccat ggcttttgtt
     1501 gccggcaaaa aacgtgttcc tagcccagcg gcaggaatga ctgctttttt gaccttcact
     1561 tgct
//
LOCUS       DAXWOO010000284         1543 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.291, whole genome shotgun sequence.
ACCESSION   DAXWOO010000284 DAXWOO010000000
VERSION     DAXWOO010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.291"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..402
                     /locus_tag="ACGE70_002902"
     CDS             <1..402
                     /locus_tag="ACGE70_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694454.1"
                     /translation="SLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELTPLDRNGI
                     AIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSRNWKEKRN
                     GYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            412..615
                     /locus_tag="ACGE70_002903"
     CDS             412..615
                     /locus_tag="ACGE70_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694455.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            826..1410
                     /locus_tag="ACGE70_002904"
     CDS             826..1410
                     /locus_tag="ACGE70_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFF8694456.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          544 a          223 c          286 g          490 t
ORIGIN      
        1 tctttaggta ttttaactat tggaattagt gtaactggaa ctacaattcc tacatttgct
       61 gatagtacaa atgaaccaac tactgtagta gataataacc aagaattaac tcctttagat
      121 aggaacggaa tagccattgg taacccaact aacgacttaa ctgttactga aaattattta
      181 atagtttctc cattacttgc tcgagcagct tctaaaacga tacgagtgac aggaacgatg
      241 atttttgata aagttggttg gccttctaga cctcctcaat cacggaattg gaaagaaaaa
      301 agaaatggtt attggtacta tggaaaattg tatttacaaa gttataaatc agctggaaac
      361 aattggattg cgacttattc aggaacctta actagaaagt agggattttt tatgccagaa
      421 tttgtgaaca caccaaattt ttatcaagat agcgatattc taagtaaaat taatttaccg
      481 aaagaagaat cagctttaca agttcagcga actttacagg agacacaaaa aattcaaaaa
      541 tcaatttcta atctagaaga ttctgaagaa aaaagaaaaa aaagagacca aaaaacatat
      601 caaaaagaaa aataatgatt ttaaaaaaag agtgtctagt cgacattctt tttttctaat
      661 tgaaagtgtt cataaaatca cccatttctg gacactttct ttttttgctt tatttttcaa
      721 aaaaacaatt catttcatgt tcataaaccc ataacaatat ctatgttcag ttaaactata
      781 tggataaagt tatgttacaa taaaattaat gaaggagtga tttttttgaa aataggatac
      841 gcccgggtat ctactggttt acaaaatttg gatttacaag aagacagcct aaataaattt
      901 ggttgtgaaa aaatttttac tgatcatatg agtggggcaa aaagtaatcg accaggatta
      961 gaaatggcca ttgattttgt tcgctctggc gatacacttg tcgtttggcg cttagatcgt
     1021 ttaggtagaa atatggaaga tttgatttca atcgtcaatc gactgaatga gcgtggtgtg
     1081 agttttcata gtattcaaga aaatattacg atggacaaat caagttctac aggacagctc
     1141 atgtttcatt tgtttgcggc atttgcagaa tttgagcgta atttgatcct ggaacgatcc
     1201 gcagcaggac gagcagctgc tcgtgcaaga ggacggtttg gtggtcgtcc tgaaaaattg
     1261 tctgaacaag atttggaatt actgaagact ttagtggata gcggtacacc tattaagact
     1321 attgcggaac ggtggaacgt ctcaagaacc accatttatc gttatttggc aaaaatggag
     1381 gagaaaaatg gaaagacaaa ttctcattag tttttgtcat ttaatgctga aaatttttgc
     1441 catttatcta gaaaagtgac aatagtatga gaattttttg taagtgtcta ctgataactt
     1501 attctcaaat gttgtaagga ttttattcat ttgttaatat taa
//
LOCUS       DAXWOO010000285         1529 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.292, whole genome shotgun sequence.
ACCESSION   DAXWOO010000285 DAXWOO010000000
VERSION     DAXWOO010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.292"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(88..1218)
                     /locus_tag="ACGE70_002905"
     CDS             complement(88..1218)
                     /locus_tag="ACGE70_002905"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFF8694457.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1278..1505)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT          431 a          310 c          243 g          545 t
ORIGIN      
        1 ataaaaggta aataaaaaag agtggatttc tccactccat gaaaagccga ctttttggga
       61 cgcttttttt tgccaagcct cacattttta tagccaaact tcttttgcga cttgctgcaa
      121 taattggatt ttcttccatt gttcttcttc tgttaaatgg tttccttctt ctgttgaagc
      181 aaacccacat tgaggactga tacttagatt ttccaatggt acaaattgtt gtgcttcttt
      241 tattcgttct tttattttct ccgtatcttc caaatctgga aacttagaag taactaatcc
      301 taaaacaact ttactttctt tgttactcca atgtcttaaa ggagtaaaat ctcctgctcg
      361 gtcagaatcg tattccaaaa agaaaccatc ataatttgtt gaaaacaact cttctgcaat
      421 gggttcatat ccaccggaaa acagccaaga agaagcgtga ttcccgcggc agatatgtgt
      481 tgtgatcgtc aaatcttctg gtttgtcttc cagtgcgtta ttgataatct ctgtacaaat
      541 atgggccatt tctttcggat caattccttt ttgaagaagt gcttctcttt tagtttgatc
      601 agctagaaac gcccaatttg tatcatcgaa ttgcaggtaa cggcaacctt tttcataaaa
      661 agcatgaatc gctgctttgt aagtagagga caagtcctca taaaaatcag atagatcagg
      721 ataaatctcc ttgatttttg aagaaccatc attagatagg atctcttgtc gtaaaatcat
      781 cgtgggacta gggatcgttg cttttgcaac aaaagtatca tctgtttcca ctacttcttt
      841 cagaaaagca aaatctttta aaaatgggtg atttggattg aaagataatt tatctactac
      901 ccgaatatta taagctggtg ctgcttttcc gtggaatgtt tgattatatc cagtttctgg
      961 aacatagcct tcgataccat ttagttcttc taaaaaatca agatgccaaa atccacgccg
     1021 aaattcacca tctgtaattc ctttgatgcc gactgctttt tggtcttcca ctaatttttt
     1081 tatttcctgg tcttcgattg cacgaagttc ttcttttgaa agtaagcctt cttgaacttt
     1141 ttttcttgct tctttgatcg attgcgggcg taagaaactt ccgacatgat ctactgtaaa
     1201 tggtctattt ttgctcattt cactcactct tttctttgtt ttttgtaaaa aaaaatcctt
     1261 ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt tttcagaaaa
     1321 aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa agactgttcg
     1381 tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc aacttctagc
     1441 tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct tctcatcgga
     1501 tactattaga ataaaattag aatagcatg
//
LOCUS       DAXWOO010000286         1518 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.293, whole genome shotgun sequence.
ACCESSION   DAXWOO010000286 DAXWOO010000000
VERSION     DAXWOO010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.293"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..487
                     /locus_tag="ACGE70_002906"
     CDS             <1..487
                     /locus_tag="ACGE70_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFF8694458.1"
                     /translation="LKEAIDQIKTAVLYPHNGLPILLMGVSGSGKTFLAEKIYEYAKE
                     QQVIESNAPFIAYNCAQYFNFSINKIVENLTILDAMKVLRLVQKAVKELEKLMGIEFS
                     NNQLFLLYMHCCSMIERILRKESVDEQADIKEYIQKEGHNYEISKYPAYKTKFESTVI
                     K"
     gene            complement(694..1218)
                     /locus_tag="ACGE70_002907"
                     /pseudo
     CDS             complement(694..1218)
                     /locus_tag="ACGE70_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1322..>1518
                     /locus_tag="ACGE70_002908"
     CDS             1322..>1518
                     /locus_tag="ACGE70_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFF8694459.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTV"
BASE COUNT          489 a          286 c          294 g          449 t
ORIGIN      
        1 tttaaaggaa gcgatcgatc aaatcaagac ggctgtcttg tatcctcata acggattacc
       61 gattttattg atgggtgtaa gcggttcagg aaaaaccttt ttggcagaaa aaatttatga
      121 atacgccaag gaacaacagg tgatagaatc aaatgctcca tttatcgcct ataattgtgc
      181 tcaatacttt aatttctcga ttaacaagat cgtagaaaat ttaacgatcc ttgatgcaat
      241 gaaggttttg agattggtac aaaaggccgt caaagaactc gaaaaactaa tggggatcga
      301 attttcaaac aatcaattgt tcttgcttta catgcactgc tgctcaatga tcgaacgtat
      361 tttacgcaaa gaaagtgtag atgaacaagc agatatcaaa gaatatattc aaaaagaggg
      421 acataattat gaaatatcaa aatacccggc ttacaaaacg aagtttgaaa gtactgtaat
      481 aaagtaagat ttaaataagc ataaacaagg taagaaccat aggggctgtg tcaagtccaa
      541 aatcttgtgt aaaaaggaaa tctcaaagta cgatgttttg ccttgttgag cctagctacg
      601 gtccaatatg ctggtaagaa gtgtcaagag caaaaagcga agcgtctctt gatacttctc
      661 accagtatat tatactattg gcggcttaat gtgctaaatc tcgttacgat caaattcaag
      721 atattttcta ggtgcagaag tccattcgtc atccttttcc atcaataaag caccgatgat
      781 acgaataaca gaatcacaat ttgggaaaat tcggacaaca cgttcacgac ggcgtacttc
      841 ttcatttagt ctttcaagaa cattcgttgt tctcaatcgt tgacggatat tttctggtaa
      901 aaccattact tgcatggcat cttcgaagcc ctcatccaat attttacagg cagttgcgac
      961 tttcttgtct tccccatatt tatccataat aagttgttta cgttctccag ccagctcgat
     1021 gcttgccgtt ttgaagatgg ctttaaagtc ttctttgata tcgcttgaga ttttcttgga
     1081 gaatttgtcg gaaatgttgc gaaggagatg tgcttggcat ctctgctagc tcgcacaaag
     1141 aaggtattct ttcacagctt ttgtgagccc tatatgcgca tccgagatga ccatctttac
     1201 tcctgaaaga ccgcgttctt tcaatccgag agtgtaaaat attttgtgta aatagaaaaa
     1261 aggaagtccc ttcagtagaa tagagttacc acaacacatt cacagaaaag aggacttcca
     1321 tatgaacgat tttactacag aaattctaaa gactctagcg aataaaggcg atttgaatga
     1381 attattccgt gtccatttgg aaaaagctgt caatacgctt ctcaaaacgg agttaacggc
     1441 tttcctcgat tacgaaaagt acgatcgcat tggttttaac acgggtaatt ctcgtaacgg
     1501 ctcctatgac cgtacggt
//
LOCUS       DAXWOO010000287         1514 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.294, whole genome shotgun sequence.
ACCESSION   DAXWOO010000287 DAXWOO010000000
VERSION     DAXWOO010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.294"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(87..971)
                     /locus_tag="ACGE70_002909"
     CDS             complement(87..971)
                     /locus_tag="ACGE70_002909"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFF8694460.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(1066..1503)
                     /locus_tag="ACGE70_002910"
     CDS             complement(1066..1503)
                     /locus_tag="ACGE70_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694461.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
BASE COUNT          476 a          325 c          235 g          478 t
ORIGIN      
        1 aaaaggctgt gacaaaagtc ttgtcacagt cccttatccc gaacaagcgg tgttctcccg
       61 ccaactcctc ggtcacaacc tccattttaa caataaacct gtaaatgtga gggcaatact
      121 ttgattgtca caggtaattt atccccttca tctccatcaa cattcgtcgt caattcttcc
      181 tcatcagcta atgacacaac taattgctca caagtaaggt aggctagatt tttcgtagat
      241 tcatctactc cttttaatag gtctggcaat gttttaagcg tatcgatcaa agaagaatct
      301 tttaaataaa ccaaatggag cttaccatca tccacttttg catttggaag taatgtttca
      361 aaaccgccga ctgagttagt tagtcctatc agcaaggtac tgctctcaat ttcttccttc
      421 tttccatcaa cttctacgtg aaacgagtag gattgtgtgg aagctaactg tttgattcct
      481 gacatgaaat aagctagttt tccaaatttc gtcttcttct ccgcatctac atcattgatt
      541 gattcaggga tagagccaat tgcgacgaca ttcataaaat agtcgtcatt tatttttcca
      601 atatccaatg gcttgactga ttcaatcgaa aaatgattga tcgcttcctc aggttctagc
      661 ggtatcccta atgctcgagc caagtcattc accgtaccca atgggaaaaa tccgaaattt
      721 ggccgatgtt cttgttcagc gattccactg attccttcgt tgactgttcc atctccgccc
      781 atgacaaaca cactatgata cccttccgta gccgcttcgc gagtaaagtt ttttgcatcc
      841 cctgcttttt ttgtatgcaa gacgaccact tcatcaaaca ccgattccaa ttttgctatc
      901 gctaattgct caaattcctt tgcttgttca ccgcctgaac tggggtttac cactaataaa
      961 acttttttca tcactttctt tcccttctga ttgatttctt taatataacg atagcataaa
     1021 gaaaaaaatt aggctgtgat gaagtactgt tcatgaaaca ttcatttatt ttttgacaag
     1081 ataaaacatc catactatga aactgaggaa aattaaaaca atgatatagg aaataaaaga
     1141 aaatgatagg cattgtatac ctaataaagt aaaaatctta ggtggcagat aaaaacacaa
     1201 aaatagcaag agaatgtaat agcgtttctt ccactttttg ttatcagacc ttatagcgat
     1261 cagaggaata ccaaatgcat aactgaaaaa ctcttgatgt actgttaata tgtcacggcc
     1321 ttgaataata aatgcgacaa tccccgctat aattagtgtg cctaacacca aaacggaaaa
     1381 ttgatgaata tattttatat acaaatgatc cattgtactt cctgcctttt tatttgtgag
     1441 aaaaacaaat ccgtatacgt gaataacttc tagaaataaa agaaatttgc aaaaccactc
     1501 catttatcta cttc
//
LOCUS       DAXWOO010000288         1496 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.295, whole genome shotgun sequence.
ACCESSION   DAXWOO010000288 DAXWOO010000000
VERSION     DAXWOO010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.295"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(457..1071)
                     /locus_tag="ACGE70_002911"
     CDS             complement(457..1071)
                     /locus_tag="ACGE70_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFF8694462.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
BASE COUNT          485 a          283 c          246 g          482 t
ORIGIN      
        1 attatctatc cttcaatttt tgtgtatttt atttctatta gaaaaaggta tcctgttgga
       61 caattttgtt atatcaggta aacttcattg caataatgat aacaaatgta ctataattac
      121 tttgtaaaga gttttgggga agactctttc atcaacgtgt attaagagac aagaaagaat
      181 ccatatggat tctttcttgt ctctttttcc actacttttt atttttctga cactacatat
      241 tcttataact catttctcat aactgtgaaa caacttataa aaataacaaa ctttaaacaa
      301 aacaagctta aaatcaaaga tgatccatct taaaataact aaaacaaaaa aagtagtcgt
      361 tgttaatcct cattaataat cgttttcttt ttttcaaacg ctccattttt atgtggaaca
      421 ttattttttt aaggaacttt gtcaacatgt ggataactat tttccacttg tgtcctggac
      481 tagctgtttt gctgtaaaat acaaaacttg ctgcagcaaa aagatcgttg caagagaggc
      541 tgttccgcag atcgctggat ctagatttgg caaagtcaag actgcatcat gtaaattggc
      601 gtggttgtcc aaaaccaaat cagcaaattc aaataatcgc aaaccactgc tatgttgcga
      661 tttgatactt cgtgacaaat caaatccagt aacaatgatt aatggatgac cattttcttt
      721 gatccactga gctagttcga tgattgccgg gtttcttcct tcattcgaaa gaagaagaaa
      781 aatgtcttct gatttgacag aaagctgatc aataattgcc tgtcctgcac cttctagcgt
      841 ttcatagaca cctccggctg gattttcaat caatatactt ggtacaagat ccggggtttg
      901 atcccaaaat tcaaatgcta caccacttag tgtgcggcta gcatatatct gcaatctacc
      961 accttgatta atttgtttcg cgatccaagt gccagcttga agcatcagcg gctcttctat
     1021 ccttaatttc tcttccaatt ggtgataata gtcaaaataa cataaactca tttatctctc
     1081 ccgcctagct atacttgaca tgaatagaaa cagctgccta atctaaggaa gtacataaaa
     1141 aaacagtagt tagacagtaa cacatgtggt ctttgctgtt tcaaacacaa aaaaggcatt
     1201 gaacgaatac gcccaatacc ttgacatagc aaataaaaca acgtgcagac catggaaaat
     1261 atctacatag gggtagaatt ttcaccctag attaggtaca ataaattagg tcgatgcact
     1321 gcattgacta tggcaaaatg atcggtcagg gtgtatgtgt gtgactggtc attccctgat
     1381 tcttagcgcc aactaagggt cagggagcct ttttttattc agctgtttct atctgaattt
     1441 attgtaagta atctcttatc caagtgcaag caagaaaata caaaaaaaca ttggta
//
LOCUS       DAXWOO010000289         1479 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.296, whole genome shotgun sequence.
ACCESSION   DAXWOO010000289 DAXWOO010000000
VERSION     DAXWOO010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.296"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(<1..487)
                     /locus_tag="ACGE70_002912"
     CDS             complement(<1..487)
                     /locus_tag="ACGE70_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HFF8694463.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FM"
     gene            787..>1479
                     /locus_tag="ACGE70_002913"
     CDS             787..>1479
                     /locus_tag="ACGE70_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFF8694464.1"
                     /translation="MRSSKYTEHFDLANPVTKVDDIPDYEMYSQTIDSLNKRFGNRVL
                     KGIEIGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDDEVKDMDPAIVIPQYFA
                     QLSEALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPILDQVIKNKLAFELNAKS
                     AYLYDNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFDDLFALLKAKGVTELAI
                     YQKRKRIMVPLPV"
BASE COUNT          458 a          246 c          258 g          517 t
ORIGIN      
        1 ccataaaaat agcgacatac ttatcattta aggctctgga tttaaaggca tttacttctt
       61 cagtcagaac tttagtcatg ttggacatgg tttgtggagt atagtgatga ccgtacattt
      121 tttcgatcaa atcagcaatt tcagacatcg taacaccttt ttcgaataaa tggataatag
      181 tggtttccaa tgtatcgttt gttcttttgt aggctggtaa agtttgttgt ttaaactcac
      241 cattacgatc tctaggtatt tccaatgtta attcaccata ttcggttttg attgatcgaa
      301 agtaagaacc gtttctcgaa ttacctgaat taaaaccagt gcgatcatat ttttcgtaat
      361 ctaaaaaagc cgttaattca gtccgtagga gtgtgtttat cgctttttct aagtgcgaac
      421 ggaataattc atttaaatcg cctttagtga ctagagtttg cacaatttct gtagtaaaat
      481 cattcatagg gaagtcctct tttctgtgaa ttggttgtcg ttaactttat tctacagaag
      541 cgacttcctt ttttgtatgg attttttcat ttacacaaaa tattttacac tctctaataa
      601 ttacacacac acaatttttt aaggtcttaa aaccatcata tatgcaaaag aagctaacct
      661 ttattactaa attagacccg tggtgctagt taattttttc ctagaggaaa atgtctaaac
      721 atgttggtaa atcaacaaaa aaacgcatat agtagtctca aaactcccaa gaaatgagga
      781 ctacatatgc gttcatcaaa atatactgaa cattttgact tagccaatcc tgttactaaa
      841 gtcgatgata tccccgatta tgaaatgtac agtcaaacaa tcgatagctt aaataagcgt
      901 tttggcaatc gtgttttaaa aggcatagaa atcggttaca tcgcttctga aaaggatcgg
      961 atcatcgact atttagccga taaagattac gatttaaaat tactcagtgt gcatcataat
     1021 ggtcaatttg attatttaga tgacgaagtc aaagatatgg atcctgctat tgtgattcct
     1081 cagtattttg cacaactttc agaagcacta gttgttatag aagcagatgt ctttgctcat
     1141 tttgactatg gttttcgggt atttggtcta tccgttgctg aatttaaaca atatgaagcg
     1201 cagtttttac cgattctaga tcaagtaatc aaaaataaat tggcattcga attaaatgct
     1261 aagagcgctt acttgtatga caatttagcg ttatatgaat atgtcatcga tttatatctt
     1321 tcacgtggtg gtaccttgtt tagtgttgga tcagatgggc attaccttga acatttccgt
     1381 ttccatttcg atgatctttt tgctctgtta aaagcaaaag gtgtgacgga attagcgatt
     1441 tatcaaaaaa gaaagcgaat tatggttcca ttaccagtc
//
LOCUS       DAXWOO010000290         1478 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.297, whole genome shotgun sequence.
ACCESSION   DAXWOO010000290 DAXWOO010000000
VERSION     DAXWOO010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.297"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(211..402)
                     /locus_tag="ACGE70_002914"
     CDS             complement(211..402)
                     /locus_tag="ACGE70_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8694465.1"
                     /translation="MNTLKDDFILAKAGSEEAIESILKRFSSLMHQQSWRNGRYDQDC
                     YQECMIAVYLAISKFEIKE"
     gene            complement(476..862)
                     /locus_tag="ACGE70_002915"
     CDS             complement(476..862)
                     /locus_tag="ACGE70_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFF8694466.1"
                     /translation="MSENDERVNAQLICFFEKVIKNTAYSYFERQKKIRELEQFDQFS
                     QHFFTENNIKIKQPVTILDVTFLIEDSRLAEIIPLLKEREQLFLIEKFVFDKTDKEIG
                     ESFGITRQGITNLKHRLYRKIQKLLI"
     gene            complement(923..1384)
                     /locus_tag="ACGE70_002916"
     CDS             complement(923..1384)
                     /locus_tag="ACGE70_002916"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694467.1"
                     /translation="MKLEEEKDAIYTQMRMILEERRRLSKDYYELKARLFALDNTNQP
                     NSLIRNEKLAKKSSTNISEEALYQQYICERGNKYRQLIPYSTISLTISSILKDAGRPL
                     SNKEIYKLLISDHKLNISYKNLTHNILPKMNVDNSVNVERAYRGYWQYRLH"
BASE COUNT          498 a          218 c          207 g          555 t
ORIGIN      
        1 tatttaataa agcatactta aaacgaagta catttgctgt tagaataatt ttggaaattt
       61 aaggaagaaa aaatattttt tactagtaaa gaaaggaaga agcatttttc aaataagtgt
      121 aaaaggaagt aaatagttta ataaaaaact ttaatattat ttttttaaaa tcgataagaa
      181 agagagaagc aaaaggcctc tcccttgatt ttattctttg atttcaaatt ttgatattgc
      241 aagatatact gcaatcatac attcttgata gcaatcttga tcatatcttc catttcgcca
      301 tgattgttga tgcatcaaag aactaaatct tttaagaata gactcaatag cttcttcact
      361 tccagctttt gccaaaatga agtcatcttt caaagtattc atagaccgtc ctcctttcct
      421 aaatttaaag ttatttagag gaggtggtcg tatacaaata atcgttaagt ggcttttaaa
      481 taagtaactt ttgaattttt ctgtaaagac gatgttttaa gtttgtgatg ccttgtctag
      541 tgatcccaaa agattctccg atttccttat ctgttttatc gaaaacaaat ttttcaataa
      601 gaaatagctg ttctctttct tttaataaag gaataatctc tgccaatctt gaatcttcaa
      661 ttagaaaagt aacgtctaaa atagtaactg gttgtttgat tttgatattg ttttcagtaa
      721 aaaaatgttg agaaaattga tcgaattgtt ccagttcacg aatctttttt tgcctttcaa
      781 aataagagta cgcagtattt ttaataacct tttcaaaaaa acaaataagc tgtgcattca
      841 cgcgttcatc attttctgac attctgctca cttcttccaa taaatattta aaaggaaaag
      901 ggacagaaag cgaaaaaaga agctaatgca atctatactg ccaataacct ctataagctc
      961 tttctacatt gacactattg tcaacgttca ttttgggtag aatattatga gttaagtttt
     1021 tgtaagaaat attaagtttg tgatcgctta ttaatagttt atatatttct ttatttgata
     1081 atggtcgacc agcatctttt agaattgagg aaattgtcaa actgatagtt gagtatggaa
     1141 tcaattgtct atatttattt cctcgctcgc aaatatattg ctgatacagc gcttcttcag
     1201 aaatatttgt tgacgatttt ttggccaatt tttcatttct aattaaacta ttaggttgat
     1261 tagtattatc taatgcaaaa agtctggcct ttagctcata ataatcttta ctaagtctcc
     1321 tcctttcttc gagtatcatt ctcatttgtg tataaattgc atctttttct tcttctagtt
     1381 tcatctactt ctcctttcat gattaacaac taaaaatatt tttaaaagga cacaaaatag
     1441 tatattatta cataattaaa aatgagcgct catattga
//
LOCUS       DAXWOO010000291         1468 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.298, whole genome shotgun sequence.
ACCESSION   DAXWOO010000291 DAXWOO010000000
VERSION     DAXWOO010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.298"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(266..592)
                     /locus_tag="ACGE70_002917"
     CDS             complement(266..592)
                     /locus_tag="ACGE70_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694468.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(579..1367)
                     /locus_tag="ACGE70_002918"
     CDS             complement(579..1367)
                     /locus_tag="ACGE70_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFF8694469.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
BASE COUNT          431 a          256 c          201 g          580 t
ORIGIN      
        1 ttttctactg ctgataagtc gccaaacttt gttaaagcag tagtcttttt ttatcttttt
       61 ttagtttacc ttaaaaaata aattaaagca aaccgctcta tttcaaggtt taagctcata
      121 gtaactatat cacgatatta caataatatt atatcacgat atcgtaatat tacaatatga
      181 agataagcga cttatcagct ttgatactac tgatttttat aaaaaaaata tcacgatatt
      241 atgatatcgt gatattgaat gtaaattaaa ttttattttg tttctttgct agtttatatc
      301 cttcaatcaa gtattcttca ggtatggact ttaatagcgc atcttctata acgtctggtt
      361 tacttttgtc tgattcagca atagtcgtcc aatatttgag tgctgacatt agaattggat
      421 ttatacgaat gctatctgct gttcttacac tacttccttg atcttttatg actttactta
      481 tatttttctt ggttaattcg atttcttcta cttcttttga tttaaaattc tttttatttt
      541 tatttcctag taatctactt actgcagctt gactgcgatt atcttctgcc atcaaaatca
      601 ccaacttctt ttaaatattc ttctgtaaat gctttatagt catcttcagc tgttgagggt
      661 ttattaaaat tgattagatc tgatccagca gcttgcgctt cgtcaacggt tattgtccta
      721 cgaatataag ttttgaaaat ttttgcacca atgactctag aaatttcggt tgctgcagac
      781 gtcatttctt tagaaatgtt ataacgctta ttatacttag taaatagaac tcctcgtact
      841 tcaagcttgt cgttcgtata ttcttttacc gtttcaatag tttcacttaa ctttattagt
      901 ccttttaatg agaaatacgc agcttcaact ggaatgataa tttcatcaga aacagtaaat
      961 gcgttgactg taagcattcc taaacttggt ggacaatcaa ttataatata atcatagttg
     1021 tccttgattg gttgaagtac tcttttaagc ctgtgttcac ttcctacttg agtaaattcc
     1081 ctctctgcag aagaaagggc tatgtcggct ggcaaaacat caattccttt atagtccaga
     1141 atagcatctg taatgtcagc ctcccctttt aatacttcat aaattgtagg ttggtttgtt
     1201 ccatctatat cgaaacctac attatctgta gcatttccct gaggatcaat gtccactaaa
     1261 agtacatttt taccgtaaac aaatttcaaa catgctgcta aattaacgct agtcgtagtt
     1321 ttagaaactc cacctttttg attggctact gacaatattc ttgccattaa aatatccccc
     1381 attcatctta tattataata taattatatc ataatattgt gatattgcaa tatcgtatat
     1441 gcattatatt gtaatattgt gtgattat
//
LOCUS       DAXWOO010000292         1457 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.299, whole genome shotgun sequence.
ACCESSION   DAXWOO010000292 DAXWOO010000000
VERSION     DAXWOO010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.299"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..785
                     /locus_tag="ACGE70_002919"
     CDS             <1..785
                     /locus_tag="ACGE70_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFF8694470.1"
                     /translation="FLTKSEKKVADFILNSGKTIIYSTMSDIKKNTKVGDATIIRFCQ
                     KIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYSTIRLLDEEKLNEA
                     IKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQAQVASLLTVNDLV
                     IIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQTAIEEFLNGGSL
                     AGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            1015..1245
                     /locus_tag="ACGE70_002920"
     CDS             1015..1245
                     /locus_tag="ACGE70_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFF8694471.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
BASE COUNT          505 a          210 c          231 g          511 t
ORIGIN      
        1 catttttaac gaagtctgaa aaaaaagtcg ctgattttat tttaaattct gggaaaacta
       61 ttatttatag tacgatgagt gatattaaga aaaacacaaa agttggcgat gctactatta
      121 ttcgcttttg tcaaaaaatt ggtttttctg gtttttctga tttaaaaatt gaaattgcaa
      181 aggaagactt tagtcaaaaa aaagaacatc cgactagtgt aaagtactat gatgaaattg
      241 ctaatagttt aacagaagct ctctattcca ctattaggct tttagacgaa gaaaaattga
      301 atgaagccat taaaatgatc actcaatcaa aaaacatata tatatttgga gtgggatcta
      361 gtggaaatac tagtcttgat ttagaaaata tgtttcttcg cgtaggagtt caagcaaaag
      421 ctgtactaga ccctcatttt caagcccaag tcgcttcttt attaacagtt aatgatttag
      481 taatcatttt ttctttatct gggaaaacga aagatactta tgattcattg aaaatagcta
      541 aaaaaaatgg cgcaaaaatt attgctataa ctaactatat tcattcacct ataggaaagt
      601 ctgcagactt agttttacaa acagccattg aagaatttct taatggtgga tctttagctg
      661 gaaaaatatc acaactttat atttgtgatc tattagttca tggttatgaa caaaacaata
      721 atattgattc agttgaatta agagaaaaag ttttgcgctc tattattgat aaaagtattg
      781 agtgaactac ccccacttag ctaaacctaa cggtttttaa cgattgaagt aggcaattct
      841 tgggatgaga gcatacttga tagcttattt tttactaata gcggtggctc ttattgacca
      901 aatatccctg tagttcctac ggttcttgat tttatctaaa gatattttta tcgtggaagc
      961 tattgatcaa cttgaaagaa aaacgtgtag ctcattatca ctagtttatc gagtatggat
     1021 gaagcaatgg atcatcctct cttaaataaa tttattaagg atctgatcct tcaaattttt
     1081 tccatgatcg atgagcagga acctactgaa tctaaacgac atcgggcaca aggaattatt
     1141 aagattgcta agcgaatgtt ttctataaag ggtgttctaa gctatacagt gttaattcaa
     1201 aagattccca atcatcttgt ttataagaac aacgttgaag attaaaaaag tgtttcaatg
     1261 ctaaaattgc aaaatacttc aatgttatga gacaaactgt ctataagaat caatttccac
     1321 ccatttcatc acttgttaaa agtttatgat ccttttgatt tttatattaa tttatctgta
     1381 tttaatacag attttattta ttagttcatc tgcctattgt attttatttt gttgccttac
     1441 catctgaagt aaatata
//
LOCUS       DAXWOO010000293         1454 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.300, whole genome shotgun sequence.
ACCESSION   DAXWOO010000293 DAXWOO010000000
VERSION     DAXWOO010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.300"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..130
                     /locus_tag="ACGE70_002921"
     CDS             <1..130
                     /locus_tag="ACGE70_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFF8694472.1"
                     /translation="TASSTIKGMETIRGIYKKNRRNGTLFGFSVSTEIKVLMGITA"
     gene            270..809
                     /locus_tag="ACGE70_002922"
     CDS             270..809
                     /locus_tag="ACGE70_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694473.1"
                     /translation="MWSLVGIVLIIFTVTAATEANYSHKIQSYKGRLVMTKKDVTRYT
                     SSRTGGLYDYAPLDALKYKDHLKKHEVKVGDRWENASHKAYNSKIVYTVEDADEQNIT
                     LPVFNYWGTVVKVNGKKSTIENDNGLVSIKGTGKRTIVEVSSTYPTTMILSLLISSIT
                     FIFLFTRYLIRCCGEMKID"
BASE COUNT          539 a          198 c          245 g          472 t
ORIGIN      
        1 tacagcctct tccacgatta agggcatgga gaccattcga ggaatatata aaaagaaccg
       61 aagaaatgga acgctcttcg gcttttcggt gtctactgaa atcaaggtat taatgggaat
      121 aacagcctaa gatatttgga gttcagagag ggcgcgtttg attttcaaac ttcgcaacag
      181 aaccgagaac cttgggtttt caagtgcttt ttggttcatt aatcttaacc atagtgtttt
      241 taaagtggaa aacaagcaac aaaaaaagta tgtggagcct tgtaggaata gttcttatta
      301 tttttactgt gactgcagca acagaagcaa attattcgca taaaattcag tcgtataaag
      361 gtcgtttagt tatgaccaaa aaagatgtta cacgttatac atcctcaaga actggaggcc
      421 tatatgacta tgcaccatta gatgcattga aatataaaga tcatttaaag aaacatgaag
      481 taaaagttgg tgatcggtgg gagaatgcat ctcataaagc atataattcc aaaatagtct
      541 atacagtaga agatgccgat gagcaaaata tcacattacc tgtattcaat tattggggaa
      601 cagttgtgaa agtaaatggg aaaaagagca ctatagaaaa tgacaatggt ttagtgtcta
      661 ttaaaggtac ggggaaaaga actatagttg aggtttcatc tacgtatcca actacgatga
      721 ttctatcttt attaattagt tcaataactt ttatattctt atttacacgt tatttaataa
      781 gatgctgcgg tgaaatgaaa atagattaga tagatattat aaatatgttg attctatttt
      841 catagcaatg gagaaaatac attagcaata tataaaagaa ggtatcaaga gtcacagtga
      901 atttattccg aaaagtgaaa agagcaaata taaaatttaa tgaaaatata gaactaaata
      961 ggaaaacgta gacgtaattt tctacgtttt ccttttcata tatattaaat attacggaaa
     1021 attatataaa ctaatttagt gttttgtttg taattcctaa ttttgctcca cttttagata
     1081 atttttaggt ctatttaact catcagacat tctatgctca tttttctcca aatttgtttc
     1141 aactctttcg tacttaattt tgcgtatttg aaaattcttc ttttagagta cttttattac
     1201 gatactagta tgatatatat ctatagcaaa cagttaaaat cttcctaaaa taagcgaata
     1261 gcattcctaa aacctataaa atttttagat gaagcgacaa gtggacaatt aataaactac
     1321 ttgaacatga aattatggat cacctttcaa atttagaaaa tggaattctt atacttattg
     1381 cgcaacattt attgattgca aaattatgtg acaaaaaaaa ctagtagaag aaagaataat
     1441 ggcttgataa taaa
//
LOCUS       DAXWOO010000294         1450 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.301, whole genome shotgun sequence.
ACCESSION   DAXWOO010000294 DAXWOO010000000
VERSION     DAXWOO010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.301"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(22..1053)
                     /locus_tag="ACGE70_002923"
     CDS             complement(22..1053)
                     /locus_tag="ACGE70_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFF8694474.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          440 a          235 c          248 g          527 t
ORIGIN      
        1 aaaaaagatc atacatttgt tttatatgga agaaacccga acgaatgccg ctaattcgtg
       61 ataagaaaaa tcaaccatct tttcccagat atcttgaaaa gaatgggatg agaggtatcg
      121 attaccacat aaataattta ccgcgtcagc atcatctaaa tatagttctg cataataacg
      181 atatgttttt ctgcatagtt ctggcgatac gtttttatat actgtaattt tgctcaaaat
      241 ttggcgaata gtccgaggag aaatttgctg atcatgtgag gaacgaaaaa caaaagaatt
      301 ttcgtccgta ttttgtgctg ttaacttaaa atagttctcc agaaatggca tcgcttcttt
      361 tggtattggt actttattca gtctgttttc ttttctattc agattaagaa tctgcttggt
      421 caaagataca tctttacgtt taagttctac tacttcatgg atctttaatc cgcaatacag
      481 aagaatggca gtaattgcaa ggttcctcgg tctattcttt tcttcaaagc tagcttctct
      541 gcttgtttga aagccttcaa tcgggataaa caaatgctca aaatctttga aaagttctgc
      601 atcgatatat cgcggagctg ctttggttgt ttccggtaaa cttgtaccca aaagagaaat
      661 ctcttcttca atcagtcctt tttgatacaa aaatcgtaaa aaatgattta atgaatattg
      721 cttccttgaa atcccgtaat agctattttc aaacaattgc tccgtttctt ggttcgctgt
      781 agtttggaac gaacgagtat gtcgatagat tttagctaag aaattctgat aatcttgttt
      841 ttctaaatgg ataatcagct gatcatccac ttcttcagaa aatacttttc tcaaataatc
      901 agtaaacaga tgaaaatctt ttgcgtattc atagcgactg cctaatgaaa gggaagaacg
      961 attttcttct aaaaactgtt gaaagacgga agggagcaag tgtgtacgtt gctttaactt
     1021 ttcttgcagt tgttttctag ttttgctgtc cattacaaat tccctccaat agtgttcatt
     1081 ttctatattt taccgcattt catcaaatga agatagacag tttgtcttga tcttaggaag
     1141 caaattttta actaagtact agctaaaaga tatgaagtaa gaagatacat ttttagttat
     1201 tttattagtg cagtcattct tttgatcttt taattgattt atcctctttt tagctatgtg
     1261 gttacttcat gagcaatgaa atggatttgg ttcgttctaa tggcaggagg ctctttcttt
     1321 tatttccgaa aaattagaaa acattttttc agaatcgatt ggctctccat ttattcaata
     1381 atggtttgtc taacgatatt gttgggataa ttcttaaaga agtattagaa atagttggta
     1441 cttcattact
//
LOCUS       DAXWOO010000295         1421 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.302, whole genome shotgun sequence.
ACCESSION   DAXWOO010000295 DAXWOO010000000
VERSION     DAXWOO010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.302"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            96..1388
                     /locus_tag="ACGE70_002924"
     CDS             96..1388
                     /locus_tag="ACGE70_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1251 family transposase"
                     /protein_id="HFF8694475.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
BASE COUNT          462 a          291 c          257 g          411 t
ORIGIN      
        1 accagcatct tttttatttt ccaaaacata tagaaaagac accttgaaat cctttagaat
       61 taatttggcg aaccaaccca aaaggaggaa tcaagatgtc ttacactcac cttatcaaag
      121 aaacattaga tattctagac ttatcagtta cttttaacga aaattgtctc acaaaagaaa
      181 aatacaaagg gcaaatctgt catatttatc gtggaaactt gatttataca gcccaagaat
      241 gcatccactg taaacaccag attgcttcag atattgtgcg ttggggtaca acaaccgtcc
      301 ggttgttaat gaacgatgtt tctgaatacc gtacctacct cgaattgaaa aagcaacgct
      361 tcaaatgcaa agcttgtcag cggacctttg tcgcagatac ttctgttgcg aaaaaacatt
      421 gtttcatcag tgagaaagtc cgctggtctg tcgtgactcg tctgaaaaaa aatacctcaa
      481 tgacagagat cgccgctcag aaaaatctat cggtctcttc tgtttaccgt attatgaagc
      541 gattttatcg tccgttgaat ccttttcgga caccgttacc taaggtactt tgttttgatg
      601 aattcaaatc agttcggggt gtttcaggtg ctatgagttt catcatgatg gatggacaaa
      661 cacagcgctt attggacatt gtcgagaacc gacaactgcc ttttttaaaa cgttatttta
      721 gtcatttttc acgagaaatt cgtgaagcgg tcgaatggat cgtgattgat atgtacgctc
      781 cttatgtatc gctcgttaaa aagctgtttc caaaagcaca gctgattatt gaccggtttc
      841 acatcgtcca acatattggt cgtacctttc gcaatcatcg aatcaaagaa acgaaccaac
      901 tattaaaaag caaagaacaa aaacactatc aattaggtaa gcaattaaaa agatactgga
      961 aacttcttca aaaagatgaa cgcaagcttg attatactcg acgcctttgg cgacctggct
     1021 tcaaagctca tttaacagag accgatattg tggatcgctt actgaaaggg agccctgctt
     1081 tgcgtgtagg ctatcagtta tatcaagact ttctttacgc tgtaaaggaa cgagactacg
     1141 tatcgtttga agaactactt accaataata tcatgcttcc cgaaggctat cagacgacct
     1201 tgagaacgtt ccagaaattt ctgccacaaa tcaaaaatgc tttacaacaa agttattcaa
     1261 atggcccact tgaatgtctg aacaaccata tcaaagtgtt aaaacgaaat gcatatggct
     1321 ttcgtagctt ttataatttc aaattgcgca tcatgattcg tcatggcaac gcattgattt
     1381 tcaactaaaa aagatcaaga ggaactgatc cacttgatct c
//
LOCUS       DAXWOO010000296         1420 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.303, whole genome shotgun sequence.
ACCESSION   DAXWOO010000296 DAXWOO010000000
VERSION     DAXWOO010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.303"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            4..978
                     /gene="bsh"
                     /locus_tag="ACGE70_002925"
     CDS             4..978
                     /gene="bsh"
                     /locus_tag="ACGE70_002925"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFF8694476.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTITPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          495 a          184 c          236 g          505 t
ORIGIN      
        1 actatgtgta cgtctattac ttatgtaaca agtgatcatt attttggaag gaattttgat
       61 tatgaaatat cttacaatga agtagtcact attactccaa gaaattataa gttgaatttt
      121 cgaaaggtaa atgatttgga tactcattat gcaatgattg gtattgccgc tggtatagct
      181 gactaccctc tttattacga tgcgacaaat gaaaaaggat tgagtatggc tggactaaat
      241 ttttctgggt atgctgatta taaagaaata caagaaggga aagacaatgt atctcctttt
      301 gaatttattc cttggatttt aggacaatgc tcaacagtag gagaagctaa aaaattgtta
      361 aaaaatatca atttagcaaa tataaattat agtgacgaac ttcctttatc ccctttacat
      421 tggctattag ctgataaaga aaaatcaatt gtcattgaaa gtatgaaaga tggacttcat
      481 atatatgata accctgtggg cgttcttacc aataatcctt catttgacta tcaattattt
      541 aatttaaaca attatcgtgt cttatcgagt gaaactccta aaaataattt ttcaaatcaa
      601 ataagtttga atgcctatag ccgcggtatg ggagggatag gcttgcctgg agatttatcc
      661 tcagtatctc gttttgttaa agcgactttt acgaagctga attctgtatc tggagattca
      721 gagtcagaaa gtattagtca atttttccat atcttaggtt cagtagaaca acaaaaaggt
      781 ttgtgtgatg ttggtgatgg aaaatatgaa tatacaattt attcttcttg ttgcaatgtt
      841 gacaaaggaa tctattatta tcgaacatat gaagacagtc aaattactgc aattgatatg
      901 aataaagaag acttagatag tcataagtta attagttatc caattataga aaaacaacaa
      961 attaaatata taaattagtt aatgtgttgt gattgattat ttaatataga tataatacaa
     1021 aaaggcaaag atattttcaa aacatttttt cttgatgtga tatccttgtc ttttttgtaa
     1081 tgtttttaac ttattgcttt tgcaaaattt atttacctgt tttctggatc aacgatagtg
     1141 tatattatgc tatccttgtt tggattttag gacgtttaaa gaggcacttt tttacataac
     1201 ttaatccata gaccttttaa gcatgctgtg agataataat aaagaagagt ttaaagcgca
     1261 ccccaaacca cttccccata agtgtattac gctttaaact cttttatatt tgaattcact
     1321 aagaagcata ccatattttt gaaaaaaagt gagaaaaaag gcttataatt aggatttaga
     1381 gtaattaatt agatgtttat tttataggac tgatactata
//
LOCUS       DAXWOO010000297         1417 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.304, whole genome shotgun sequence.
ACCESSION   DAXWOO010000297 DAXWOO010000000
VERSION     DAXWOO010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.304"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(150..1337)
                     /locus_tag="ACGE70_002926"
     CDS             complement(150..1337)
                     /locus_tag="ACGE70_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFF8694477.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          412 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttc
//
LOCUS       DAXWOO010000298         1409 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.305, whole genome shotgun sequence.
ACCESSION   DAXWOO010000298 DAXWOO010000000
VERSION     DAXWOO010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.305"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..1359
                     /locus_tag="ACGE70_002927"
     CDS             <1..1359
                     /locus_tag="ACGE70_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFF8694478.1"
                     /translation="LFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLPITEKE
                     HLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEKEGLSS
                     FKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRDNITNW
                     HIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKDSQHPI
                     VYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFFEAIPK
                     GKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWMATIDP
                     VAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHSTSLEG
                     ARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDVQQHFS
                     RN"
BASE COUNT          466 a          255 c          299 g          389 t
ORIGIN      
        1 ctttttagtg atgaagaaat ttctgtaagt gttccagaag cagctgccac cattattcca
       61 gtaaaaggac atcaacgagt tgtaggaacg aaagcagaca aaatcaaaca cttacctata
      121 acagagaaag aacatctcct tcctttggaa gaacaatttt gtgaacattg cggttcacag
      181 atgaaagata tcggacggac aaaagtacga gaagaaattc gtttccatca ggctatgttg
      241 gattgtctca cccattatca acatacctat tgttgtaaaa gctgcgaaaa agaaggactt
      301 tcttctttta aaaaagcgat cgttcctaaa cctttgattt ctaatagctt aggttccaat
      361 agtttagtgg cggaaacgat ccggatgaaa tttggtcaga aagttcctgc ctatcggcaa
      421 gagaactatt ggaaacaaac acatgggttg gatatttcga gagataatat caccaattgg
      481 catattaaag cagtgcaaaa cgcgttagac cctttaggag aacgattaag agtctatctt
      541 aatcaagaag agattctaca cggggatgaa acaagttatc gtgtgattga aagtgccaaa
      601 acagatacct attactggca gttttgtaca ggaaaagaca gtcagcatcc catcgtttat
      661 tatcaccacg acgaaagtcg tgctggagat gttccgaaaa cttttctcaa ggagtttaca
      721 gggtatttac actgtgacgg ctatagcggg tataatgccg tggaaagtgt acgacttgtc
      781 tattgtttcg cgcatgttcg gcggaaattt ttcgaagcaa ttccaaaagg aaagaaaaac
      841 acggatattc ccgcagctca agcagtcaaa caattggata aatggtttgt cctagaaaag
      901 aaatggaaag acttttctcc tgagaagcga ttaagctgtc ggcaacaaga actgcgtcca
      961 ttatttattg ctttttatga gtggatggca acgatagatc ctgttgcaaa atccaagttg
     1021 gatgcagccg ttcaatatgc gtgtaaacta cgaagtggtt ttgaacctat ttttgaagat
     1081 ggtcgtttag aactcacaaa taatcgtgcg gagcgaaata tcaaagagct ggtgatagga
     1141 cggaaaaatt ggttgcattc cacaagtctt gaaggagcac gaacttcagg aatcattctg
     1201 agtgtatata aaacagcaga acttaacgga ttgaatcctg taaagtatct agagtttcta
     1261 tttgataaga taccaaatct tcccgtactt tcagcagaaa ctttagatca actgttgcct
     1321 tggaacaagg atgtacaaca acatttttct cgtaactaaa aacgccaaac attcccgatt
     1381 ctattctata ggtcgggtta tttgacgta
//
LOCUS       DAXWOO010000299         1404 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.306, whole genome shotgun sequence.
ACCESSION   DAXWOO010000299 DAXWOO010000000
VERSION     DAXWOO010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.306"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(713..1222)
                     /locus_tag="ACGE70_002928"
     CDS             complement(713..1222)
                     /locus_tag="ACGE70_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694479.1"
                     /translation="MKKVLFAVSLVVNIALAGGLFYTITEMNKLDDQRESLAISNEQY
                     KRKLDEASSTNVDTQEAVESSTIVYSNESSETLTNSERSTAPATETVSQKATITIGSK
                     VFTFIVNGLTIEHDSSRTGKMTELSHEFPVIVTTPSATLNGILVQDNNDNNCYIMVDG
                     QTLYRGTYQ"
BASE COUNT          453 a          248 c          215 g          488 t
ORIGIN      
        1 tacaccaaaa accactagct ggcgggctag tggttttctt ttacgttcta ttcagattaa
       61 cgggtaggct tactgtcttt tttggcaata attttgcaat caagccattt acagatgtaa
      121 tgcgcggata cttctgctaa gattgcaaaa aacatatcga aaaggtaatt cataatacaa
      181 cctcctcccc ttgccataat aggccggggt agcagacagc ataaatatta taccataact
      241 gaaaatttca ttttagtaca tttcaatata ttgatgaacc agcacaataa aacatgccat
      301 gactaacaga aaaaaactta ttaaaccaga tataacttgt aaacatccaa gtatatagaa
      361 aacgtaaatt ttctatatac ttggatatgc tggaatgttt aagagaataa ttacatttct
      421 agaaaataca ataaaaatta caggataatt agccatatcc tacctcctct agcagttgct
      481 caaaagttag cactaagtaa cttctttttt gttgctggta acaatttatc aatactaaaa
      541 tagcgtttaa gtatctaata aatactaatt ttaaagctct taattattat gaaacacgat
      601 ataacaatat tgctatatcg tgctattgta atattataca tttgaagaca gcgtattctt
      661 gttctaatat caggaatttt ttataaatat cagtcatatt aagactcaaa atttattggt
      721 atgttcctct gtatagagtt tgaccgtcta ccattatata gcaattatta tcattgttgt
      781 cttgcaccaa tataccgtta agggtagcac taggagtagt aacaatcact ggaaactcat
      841 gagaaagctc ggtcattttt cctgttctgc tagaatcgtg ttctattgtc aagccattta
      901 ctataaacgt gaatacttta cttcctattg ttatagtagc tttttgagaa acagtttctg
      961 tagctggagc tgtgcttctt tccgaatttg ttagggtttc actggattca ttgctataaa
     1021 caattgtaga ggattctacg gcttcttgag tgtccacgtt ggtgctgcta gcttcatcta
     1081 gcttcctttt gtattgttcg ttactgattg ctaagctttc ccgttggtca tcaagcttat
     1141 tcatttctgt aatggtataa aataatcctc ctgctagtgc tatattaaca actaaactaa
     1201 ccgcaaataa tacctttttc attcttcctt ctcttctttc tctttttgta attccttact
     1261 tttggatagg ctacaattaa ctagacagaa aaattaaggt gtgtagacta gaagaaaaca
     1321 taccaggagg aatttttatg tctaagagaa cacgaagaac tttttcacaa gaattcaagc
     1381 aacaaatcgt caatctttac ttag
//
LOCUS       DAXWOO010000300         1401 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.307, whole genome shotgun sequence.
ACCESSION   DAXWOO010000300 DAXWOO010000000
VERSION     DAXWOO010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.307"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            675..>1401
                     /locus_tag="ACGE70_002929"
     CDS             675..>1401
                     /locus_tag="ACGE70_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFF8694480.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFKEITGISSEKFQKTFPTRQLAIKA
                     ENEMKKKIAKVLREENANSLELKGQIKFKEFYKSKWLPRYELGQTTRSHKAPSDITII
                     NTKNLFRLHILPIFGEYAMSYLNANTEIISDELTKKSKEYANIKIIKCYVRSMFDIAE
                     VLNYIEFNRTTKIIQSITAPKKVALEEKRIREGKQALSSKELTDWLDAVRDDFNDQLL
                     SFHDYTLFMLTLYLGDRKSETYAL"
BASE COUNT          518 a          201 c          223 g          459 t
ORIGIN      
        1 tattttaaat ttttacatac ctgtttatga aaaactaaat aatctgtcaa aaaaatatga
       61 ttgttcattt ctaaatatgt tttccaattt taaaatcttt tttagcattt ttgtttcctt
      121 ttcatatggt ttttcgttgt taatagtgac taggatgata ttcctatttt gaccattgac
      181 aattaaatat aatttttgaa taacgttccc tgctattcga gctaagaatt caagtacgcc
      241 atgacctatt tagcgataac tacttagaga tttctttttg ttttgggaag acaaaatgcc
      301 atgacaatag cagtagataa tgatacttcc gtaaggctcg ccgtaaactg gggagaggtg
      361 aaattcctat gtgcttaccc aagtcaattc aaaaatttta gttcatttat aaggggatga
      421 ttttattaaa tcaatagtct atctaaacaa aaaacaaacc tgtgagtttt taggaattga
      481 taacaatact ttagataaat ggattcgtat atataatttt cccgtaataa aaatagacgg
      541 tataattcgc tttgatgtag aagatatcaa aggctttatg gatacgttca aaaagatttg
      601 aattttactt ttgactgcat atactaaaag tagcgaaata tactgcctta cttttttatt
      661 aaggagtgag gaagttgcct acgaagcttt caaatggaaa atataaaact aatttgagat
      721 atccaaaaaa atttaaagaa attacaggta tcagttcaga aaaattccaa aaaacatttc
      781 caactaggca attggctatt aaagccgaaa atgaaatgaa aaagaaaatc gcaaaagtct
      841 taagagagga gaatgcaaat tcattagagc tcaaaggtca aataaaattc aaggagtttt
      901 ataaatctaa atggcttcct agatatgagt tagggcaaac tactagatcc cataaagctc
      961 caagcgatat aaccatcatt aatacaaaga atttatttcg attgcatatt ctgcctatat
     1021 ttggtgagta tgccatgagc tatcttaatg caaataccga aataatatca gatgaactca
     1081 caaaaaagtc gaaggaatat gcaaacataa aaatcattaa atgctatgtt cgttccatgt
     1141 ttgatattgc agaggttcta aattatattg aatttaatcg tacaacaaaa ataattcaaa
     1201 gtattactgc acctaagaaa gttgctctgg aagaaaaaag aatacgagaa ggaaaacaag
     1261 ctttatcttc gaaagaactc actgattggt tagacgctgt aagagatgat ttcaatgatc
     1321 aattactttc ctttcacgat tacactcttt ttatgcttac tttatatctt ggggatagaa
     1381 agagtgagac ctatgctttg c
//
LOCUS       DAXWOO010000301         1348 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.308, whole genome shotgun sequence.
ACCESSION   DAXWOO010000301 DAXWOO010000000
VERSION     DAXWOO010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.308"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(133..432)
                     /locus_tag="ACGE70_002930"
     CDS             complement(133..432)
                     /locus_tag="ACGE70_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFF8694481.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            783..>1348
                     /locus_tag="ACGE70_002931"
     CDS             783..>1348
                     /locus_tag="ACGE70_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFF8694482.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLK"
BASE COUNT          457 a          218 c          188 g          485 t
ORIGIN      
        1 aaatacaata taaagaaaca tctgtttctt tatttagaat aaaaaaatac agactctgtt
       61 caaataaatg aacctagcct gcattatgag attgactata ctttttaatt cattgagatg
      121 aataactcgt ttttataaat gcccaaaagt aatcggtgcc gaagccattc ctcgacctaa
      181 ttctttttca aattttccta ttgttgatat ctccatataa aacggtgata ctggtttaga
      241 caattctccc ctgattgcta acggacgtaa actacgttcc aaattattta tgactttagg
      301 ataatactga cctttttcta agcctgtctt tgcgtctatc aaaagtatac gttccttttc
      361 tttgatctct ggatttaaaa cgagatcata taattttagt accatgtttt ctatttcttc
      421 acttttagac atcacgattt cctcccaaaa atatatttct tcccactaca ttctataaac
      481 aaagtatacg ctacatctag atttttttaa atcattactg gattgttaaa acctttcaat
      541 acactgttat ggcaacgatt tttattttaa aaacattcta aattaaatca gtaaaaattt
      601 ttattctaag atttttatta gaattacgcc taatttgtgt ctctcagggc aattctaaga
      661 acgtttcaag agatagcgac actatcgtta tcgaaaaagg cgaaaaccta tctcattttc
      721 tgctaatgta agttggttta gcttttgtta atatgacttt atgaaaatgg aggtaattaa
      781 atatgatttt tcctaaagtt aatgaagcaa gcttgaataa aggatccttt tttcatatct
      841 atgaaaacga agattttgaa ctgtataata tcctttcaca aaaagaggtc atcgaaaacc
      901 aaataatctg ccatccaatt atttacaaat ctattttcaa gtatgcattt tttgcttcca
      961 atgattttca aagaatggag attaccgact gtatattcga aaactgtgat ttttcaggct
     1021 gtatattgac aaaaagcatg ttgcatcgcg tgaaatttgt caattgtaaa ttaacaggta
     1081 ctaaattgat ggaaagttat attgggaaca cgttattcga gaattgtaaa atggactatg
     1141 taaatttatc tggttcaaac ataaaagaaa gtacctttga gcactctgtc ttatcaagcg
     1201 cagattttgt agactgttcc ctcactaaaa taatgttttc tactaatgat ttagaaaatg
     1261 ctagcttttt tgatactgat ttaaaaaata tcgatttaag tagcaactcc tttgaaatga
     1321 ttgaagtacg ctcggaacat ctaaaaaa
//
LOCUS       DAXWOO010000302         1345 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.309, whole genome shotgun sequence.
ACCESSION   DAXWOO010000302 DAXWOO010000000
VERSION     DAXWOO010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.309"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(33..362)
                     /locus_tag="ACGE70_002932"
     CDS             complement(33..362)
                     /locus_tag="ACGE70_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFF8694483.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(439..711)
                     /locus_tag="ACGE70_002933"
     CDS             complement(439..711)
                     /locus_tag="ACGE70_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFF8694484.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(711..974)
                     /locus_tag="ACGE70_002934"
     CDS             complement(711..974)
                     /locus_tag="ACGE70_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFF8694485.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            1208..>1345
                     /locus_tag="ACGE70_002935"
     CDS             1208..>1345
                     /locus_tag="ACGE70_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFF8694486.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LK"
BASE COUNT          431 a          258 c          173 g          483 t
ORIGIN      
        1 aatacttaat gacatgtttt gaaggaacga tttcataagc caatttctgt tgccaactcc
       61 tctttagtca ttactttaga attagtaagt gtatcttcag caatatctct aaaggaagaa
      121 tgcattttgt tttttctatt cttaaattct acaagaatct catctttatc gtagccttct
      181 gcaattatat cagacaaaat atcttcgcta aaatcataaa attctttttg tggttcaaca
      241 aattcataaa gaataccatt ctctaccaac tttgcttcaa cttcgactcc attaggaaca
      301 ttaaaatctt taggtactgt caacatgatt gaattgcctt gttttcttgt ttttgtaatc
      361 atttaaatcc ctcatttcta acagtatgta attacttagt aaatacatta taccacgaga
      421 taatgtattt acaagtaatt actgtgactc gtaatgcgtc cttgctgaat aaatttcaac
      481 cacgtgttca tcttcatcta ccttataaac aacccggtgt tgtctattca atcttctaga
      541 atacctacct tcatgtgtag gttttaactt ctcaaatgat tgggtaggga gatagggatc
      601 ttcctttaaa gtctgaataa ccttctcaaa ttgaggtttc aaattagact gtttgatttt
      661 ctttaaatca gctttagccg agtttttaat catcacagaa tagttattca ttataagtta
      721 tcccaatcaa tttcatcaac attagtaaat ccactatcat ctttttctcg cttacgaaca
      781 acacccaaag tgcctatttg ctcaagtaat agcgtttctt tgattgcttt ccaatcatcc
      841 aaaccaataa gcaccgcatt attttcactg cgctcactaa tgatttcaat ttccgtatga
      901 ttctcattta catctttcaa caacttataa aaatttttcc gtgctgagct aggatttaag
      961 actgtttttg ccatatctac tccccctcta atttatatct ccattctaac gtacttaatt
     1021 aagtacgtaa attcgattgt tttgtttaaa ttggagtacc aaaacagcat tgtaaagtgt
     1081 tatccgtttc ctttataccc gtggtgctag ttaatttttt cctagaggaa aatgtctaaa
     1141 catgttggta aatcaacaaa aaaacgcata gagtagtctc aaaactccca agaaatgagg
     1201 actacatatg cgttcatcaa aatatactga acattataca gatactttta taacattcaa
     1261 ggaaattatg agaactgttt ctaacatata ccacaattgt gtaccagata agattaaaaa
     1321 ccgaagaaat actgatcaac tgaag
//
LOCUS       DAXWOO010000303         1338 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.310, whole genome shotgun sequence.
ACCESSION   DAXWOO010000303 DAXWOO010000000
VERSION     DAXWOO010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.310"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..134
                     /locus_tag="ACGE70_002936"
     CDS             <1..134
                     /locus_tag="ACGE70_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HFF8694487.1"
                     /translation="DESQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            261..845
                     /locus_tag="ACGE70_002937"
     CDS             261..845
                     /locus_tag="ACGE70_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFF8694488.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            959..>1338
                     /locus_tag="ACGE70_002938"
     CDS             959..>1338
                     /locus_tag="ACGE70_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HFF8694489.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTV"
BASE COUNT          536 a          199 c          240 g          363 t
ORIGIN      
        1 ctgatgaatc tcagtttaat ctcgaaaaat ttatggagta ttattctatt cagaataagc
       61 tcagatacac ttgttatgct gtctgccaat gctattttaa gaaaaaatat tcttacgcaa
      121 ggcaatatgt atgaatacat caagaattat taaaaaaacc gatttcagca aaacattaaa
      181 aaagcccgtg ctggcacacg gactcatacc tcatttctga gatcgcaaat atattataac
      241 aagaagtgag gaatatttaa atggcaaaaa aagttatggg tcaagacgga aaaatgtata
      301 aagttagtaa acctttttac aaaaaagtat ggttttgggt attagcagta attttaatta
      361 taattattgg ttctgcttta aatggtggat cagatagtaa taaagcaagt gataatggtg
      421 gcgaaaaagt aactaaatct tcaacctctg cttcatcttc taaagaagaa aaaagcgata
      481 ccttctataa gattggtgac actgtaaaag ttggtgatgc tgaatataca ctaaatagtg
      541 tagaactaac tgatgaaaga aatcaatttg aagaaaacca acctgcacaa gtagtaaaaa
      601 ttacttatac cgtaaaaaat gatggcgatt cagatatccc tgtaggtaca gatgtggaag
      661 tatatggacc agatgacaaa aaatcagaga catatgccaa cgaaaataca atgggatctg
      721 ttgctccagg aaaacaaatg gatgtaactg ctcatttcac gttaaaccaa aagggagaaa
      781 tagaaatcca cttctctcct ttagtatcat ttgaaaaagc agctattttt aaagcaactg
      841 tataataaat aaaaaacacg cccaccgtcc aaagcaagcg tgttctaaga aaaacaaacc
      901 tatacagtag gcttctttat agttcctatt gtatcagaga aagagagctg attcaattat
      961 ggcaagatta gtcaaacgtg gaaatagttg gcaatacgaa atttcataca aaaaagatga
     1021 cggaaaatac acgaagataa gaaaatcagg atttaagaca aaaggagaag caaaagatgc
     1081 cgccaacgaa ttagaatata acttgaacaa aggccttaaa ggggatcgca aaaatctatt
     1141 attatcagat tactttgagg attggatgca actttataaa gaaggaacag tatctcctat
     1201 cacttataga aaatacgaag atacgttaat gaacataaag aaatatatgc cagcggtatt
     1261 gatttctgat ttagatagag tgggatatca acgcttttta aataagtatg ctaaagacca
     1321 tgtaaaatct accgttat
//
LOCUS       DAXWOO010000304         1334 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.311, whole genome shotgun sequence.
ACCESSION   DAXWOO010000304 DAXWOO010000000
VERSION     DAXWOO010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.311"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            86..1165
                     /locus_tag="ACGE70_002939"
     CDS             86..1165
                     /locus_tag="ACGE70_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFF8694490.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
BASE COUNT          359 a          357 c          369 g          249 t
ORIGIN      
        1 agccatttgg aaataaaaaa acgccacgtg aattccatgt tatattgaag ttaccacaac
       61 tctcaataga caaggatgaa ttcacatgac gcaaaaccat gataacacag tttcacgcaa
      121 gggaaaacac ctaacctact cagaacgttg ccaaatcgcc atcctgaaaa aggaaaacta
      181 ttccaaccgt caggttgcgg atgtcctggg ctgcgtgccg cagaccataa acaacgaaat
      241 caaacgcggg accgttactc agcttaaccg ccagaatcag aacgggaagg tctacgacta
      301 ttatcatgac gtctacgacg ccgatgccgg gcaggcggcc tacgagaaga accgcttgaa
      361 ctgcggcaga cggcccttat gggccgacac ggacgccttc attgaatggg cggacgacat
      421 gatgctgttg gaaaaatggt ccccggacgt ggtggtgggc tttgccagaa aacacgaatt
      481 gttcgacccg gccattatcc ctgggaccac caccctctat cagtggatcg accgtggcat
      541 catgaaaacg aagaatatgg acctgctaga aaaactgtcc cgcaaaccga aatcgtcccg
      601 tcatggcaac cgcccgaata aacgggtgct agggaaatcc attgaagagc gcccggaggc
      661 ggttggtacc cggaaaacct tcggccattg ggaagtcgac acggtcgtag gaaacaagac
      721 caagtcggat gccgtgctgc tgacgttggt ggaacggcag acacgcttcg aggtcattct
      781 gaagctggcc gggaaggatg ccgaatcggt caaccaagcg gtccgacagc tgagagaacg
      841 ggctggtgcg tacttcggcc agctgttcaa gacggttacc tcggacaacg gctcggagtt
      901 cgcgggactg cacgatgcct tgcaggatgt ggcaaacatc tacttcagcc acccctatgc
      961 ttcctgggag cgcgggacga gcgaaaacca acacaagctg attcgccgtt tcatccccaa
     1021 gggcagccca atcggcaacg tgagcgaaaa ccaaatcttg cggattcagc gttggatgaa
     1081 cgattacccg cgaaagattt tgggctatgc aacgccgcat gacacgttcg tccaggcatt
     1141 taggcaggaa cggttgacgg cttgacacgt gggcccttga caacgagcct ccctcggcat
     1201 ggcttgtgtc tggaaagctg gccggggcca gccagtcggg agactaccat gcctcagctc
     1261 attgtcaagg gcccactcga ttagaaaaat tagacggttg gtggtaacga gcaaccgggt
     1321 caccatgggt ggct
//
LOCUS       DAXWOO010000305         1312 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.312, whole genome shotgun sequence.
ACCESSION   DAXWOO010000305 DAXWOO010000000
VERSION     DAXWOO010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.312"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(156..1297)
                     /locus_tag="ACGE70_002940"
     CDS             complement(join(156..1069,1069..1297))
                     /locus_tag="ACGE70_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFF8694491.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
BASE COUNT          323 a          263 c          278 g          448 t
ORIGIN      
        1 gacacgattt gtgagaattt tttcgagatt ccagcgggtg agtatctcat cgtttcgacc
       61 agcggtcaaa ccaaaaccgt ttgaccgctg atcgaagcga tgaagttgaa accctgtgga
      121 aatctcgcca aaaaaatgta agtggtagct aggaactaag cttgcaatgc caactcctcc
      181 atctgatttg gtgttaaata gttgatacta ctatggattc gcttgcgatt gtaaaatcct
      241 tcaatgtatt gaaataacgc taaataagca gcttcaaagt tttcatacgc ttttgatgga
      301 tagacttctt cttttttgag tgaggcgtga aacgattcga taccggcatt atcgtaggga
      361 cagcccttac gactgaagga gtgccgaatg ttcaattcat tcagctttgc ctcataggct
      421 tcgctagtgt actgagaacc caaatctgag tggatgatca agttctcgga gggtgtttgg
      481 tttaagagcg cattgtcaag ggtttcgata accaattctt tcgtcatctg cttgccaaac
      541 ttccaaccga tgactttctt tgtgtggagg tcttgaatag tggagaggta ggtccagcca
      601 tcttttaggg tatgaatata ggtaatatct gtcacccatt tttgattgat cgacgtagtg
      661 gtaaaatctt ggtttaacag gtttttacgt ccttcacttt taactttcgc ttgtttgcca
      721 ggcttgtact tcttgagggt gactgaacgc aagcctaatt ttctcatcag tatttgtact
      781 ttctttaagg acaaggtctc gccacggcca acaagaatct tatgaatttt aggcgcacca
      841 tagataccct tgttttcaaa gtagattcct gaaatcaagc ggtttaagcg tttgttttct
      901 gattggcgct tggaaggttt ctttttcttc cagtcatagt agctggattt cggtgcttcc
      961 aaaacggtac acatttgttt tacgtcatga tcatgaacat ttgattgaat aaattcgaag
     1021 acatcactca tttttacttt tgagcgaata tggttaaagc cttttttagg atagtgtttt
     1081 cctcctgcat gcgggccatt tcctttttca tcgctaggat ttctgcctca gtcatgccgg
     1141 ttgctacatt ctttgtatga agcttcttcc aacgataaat cgtttgtgta gcgataccat
     1201 attgcgctgc cagttcttcg accgattggc cagactggtt taaatctaag atcatcttct
     1261 taaagtcttc atcaaatttt ttcttggctt ttgccatcgt ggacacgtcc tt
//
LOCUS       DAXWOO010000306         1304 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.313, whole genome shotgun sequence.
ACCESSION   DAXWOO010000306 DAXWOO010000000
VERSION     DAXWOO010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.313"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            74..>1304
                     /locus_tag="ACGE70_002941"
     CDS             74..>1304
                     /locus_tag="ACGE70_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFF8694492.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGF"
BASE COUNT          410 a          258 c          253 g          383 t
ORIGIN      
        1 taaaaaaata tagaaaagac accctgaaat cctttagaat taatttggct accaattcat
       61 aggaggattc aagatgtctc atactcagct tatcaaagat accctaaata ttctagacct
      121 aaatatctat ttcgaggaaa attgtttaac taaagaaaaa tacaaagggc aaatttgctt
      181 gatttacaaa ggaactcttc tgtacaagcc agaagagtgt tcccattgtc tttgtgtcgt
      241 cccctctagg atcattcgtt gggggacgac aaccgttcgg ttgttactga atgatgtgtc
      301 tgagtatcgc acttatctgg agctgaagaa acaacgattt aaatgtaagt cttgccaaag
      361 aacattcgta gcggatacct ctgttgctga aaaacactgt ttcattagtc aaaaagttcg
      421 ttggtctgtc attgctcgct taaaagagaa tacttccatg acggaaatcg ctcgtcagaa
      481 aaatatttcc acttcttcgg tctatcgtgt catgaagcga ttctaccgcc cattgaatcc
      541 tttcaaacag actttgccaa aggtcttatg ctttgatgaa ttcaaatcgg ttcgaagtgt
      601 tgccagtgca atgagtttta tcatgatgga cggacaaagc catgcattat tggatattgt
      661 cgaaaatcga cgcttgcctt atttagaacg ctatttttct cggttttcat tagcgactcg
      721 tgaagccgtt caattgattg ttattgatat gtatgcgcct tatgtttcgt tggtgaaaaa
      781 gcttttcccg aatgctcagt tgatcatcga tcgtttccac atcgtccaac atattggcag
      841 aactttcttg aatcatcgag tgaaagaaac cacggtacga cttaaaggaa attcccactc
      901 tcaacgaggg ttagggaaaa aattgaagcg ctattggaaa ctcctacaaa aagaagaagc
      961 aaagttaaac tatgaaaaac gggtttggcg tgccagtttt aaagattatt tgactgaaag
     1021 tgagattgcc gatcgactgt tagctgcttg tcccaactta cgccaaggct atcagttata
     1081 ccaagacatt ctttatgcgg taaagaaaag agatcagtca ctatttgagg attgtttaac
     1141 tagagacatt agcggtctgc cagaaaccta tacaaccact ttacgaacgt ttaaaaagtt
     1201 tctgccacag atccaaaatg cgcttcacta cagctattca aacggaccat tggaatgctt
     1261 aaacaaccac atcaaagtat taaaacgcaa tgcgtacggc tttc
//
LOCUS       DAXWOO010000307         1284 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.314, whole genome shotgun sequence.
ACCESSION   DAXWOO010000307 DAXWOO010000000
VERSION     DAXWOO010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.314"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..984
                     /locus_tag="ACGE70_002942"
     CDS             <1..984
                     /locus_tag="ACGE70_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HFF8694493.1"
                     /translation="NYRIIWLIKKEAIITDLPDYIKRIDSDSFLAAFYLYTAKYWIDD
                     SRKTIKYAKRKKQFYFQTWHGTPLKKIEFDAKDKLPKRYLSYAKKDSESITYLLSGNR
                     YSSEKYVSAFNIVPSKILEVGTPRNDELASSKEQVFDLKKKQINVLFAPTFRNNIEDN
                     GITQLEWLGVDNLREFFKKQQQELNLMTKFHPNVHSKLATDSKSIEYMKKHQITLIND
                     GIPLEMLFEEIDVLITDYSSIFFDFALTKKPIILFNYDEKEYSLERGFYIQLSELPII
                     SVQDSYGLVDVFKNQQEKLLKSSQELLDYIGNYETGASTEKVLELLERDIK"
     gene            981..>1284
                     /locus_tag="ACGE70_002943"
     CDS             981..>1284
                     /locus_tag="ACGE70_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFF8694494.1"
                     /translation="MKKTVKDIFYNAIYQVFLIVLPLLTIPILSRRIGSTGLGIYGYV
                     FSISQFLMTVIAVGMNPFRIRNIAKSRKDKKALSLQFWNIYFLQFLIGLSVSFLYIA"
BASE COUNT          470 a          164 c          210 g          440 t
ORIGIN      
        1 aattacagaa ttatttggtt aatcaaaaaa gaagcgatta taactgattt accagattat
       61 attaaaagaa ttgattccga ttctttttta gctgcctttt atttgtatac agctaagtat
      121 tggatagacg atagtcggaa aacgattaag tatgcaaaaa ggaaaaaaca attttatttt
      181 caaacatggc acggtacgcc tttaaaaaaa attgaatttg atgcaaaaga taagttacct
      241 aaacgttacc taagctatgc gaaaaaagat agtgagtcaa ttacatattt attatctggt
      301 aatcgctata gttctgagaa gtatgtttca gcattcaata tcgttccttc taaaatatta
      361 gaagtaggaa ctcctagaaa cgatgaatta gcctctagta aagagcaagt gtttgacttg
      421 aaaaagaaac aaatcaatgt cttatttgct ccaacttttc gaaataatat tgaggacaac
      481 ggaattacgc aactagaatg gttaggtgtg gataacttac gtgaattttt taaaaagcaa
      541 caacaagaat taaatttaat gactaaattt catcctaatg ttcatagtaa attagcaact
      601 gattctaaaa gtatagaata tatgaaaaaa catcaaatta ctttaattaa tgatgggatt
      661 cctcttgaaa tgctttttga agagatagat gttttaatca cagattattc atctattttt
      721 ttcgattttg cgctaacaaa aaaaccaatt attcttttta actatgatga aaaagaatat
      781 agcttagaaa gaggttttta tatacagtta agtgagttac caattataag cgtacaagat
      841 agttatggcc ttgttgatgt ttttaaaaac caacaagaaa agcttttgaa aagttctcaa
      901 gagttacttg attatattgg aaattatgaa acaggggcaa gtacagaaaa ggttcttgag
      961 ttgttagaga gggatataaa gtgaaaaaaa cagtaaaaga tatattttat aatgcgatat
     1021 atcaagtctt tttgattgtt ttgcccttac ttactatacc tatacttagt cgtagaatag
     1081 gttcaactgg tttagggatt tatgggtatg ttttctcaat atcacaattt ttaatgactg
     1141 tcattgctgt gggaatgaat ccttttcgta ttcgaaatat cgctaaatca aggaaagata
     1201 aaaaagcact atctttacaa ttctggaata tttactttct tcaattttta ataggcttga
     1261 gtgtttcctt tctctatata gcct
//
LOCUS       DAXWOO010000308         1276 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.315, whole genome shotgun sequence.
ACCESSION   DAXWOO010000308 DAXWOO010000000
VERSION     DAXWOO010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.315"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            230..1087
                     /locus_tag="ACGE70_002944"
     CDS             230..1087
                     /locus_tag="ACGE70_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFF8694495.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            1088..>1276
                     /locus_tag="ACGE70_002945"
     CDS             1088..>1276
                     /locus_tag="ACGE70_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694496.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTK"
BASE COUNT          475 a          185 c          258 g          358 t
ORIGIN      
        1 gtctacatga ctaaagtcgc gagtattctc tgcttctcga taaattaaat aacgtcatta
       61 ctttgaggaa agctataaat caatatattt cagcttttcc ttatttatgt actacctttt
      121 acttattaat tataaggtag tacatatata tctttttttg gtagtaacta aaaataaata
      181 tgttataata gtatcaaatg aatattgcta aaagaaaaga ggtaggaacg tggtagcgaa
      241 gaaatatgta atcggtaatt ttaaaggtgg cgtaggtaaa agtacgtgcg cgcaaatgtt
      301 agggtttgaa tcagcagtta atgaaggaaa aagaaccttg ataattgact tggatatgca
      361 aggaaatact tctgatgtga tgaacttaac gcatatgaac ttcactaacg aagaaggagg
      421 cggagaaggt gaaccgattg tttacgaaaa tactgtgact gacgtgttga taaacaatct
      481 tccacctaaa gaggctgtct ataaagtaat caataacctc tatatacttc cagcagacat
      541 gtcctttgaa atgtatgatg attggatcaa agaccaatat aatgattcat tatcccaatt
      601 caaatatatg aaagaaaagc tagaaccatt atttgatgag tttgacgtca tctatttaga
      661 tgtcccacct tctatttctg tatatagtaa atccgcgatg tacattgcgg actgggcaat
      721 cgtcgtctta cagacccaag taaaatcgat gagaaatgcg atgcaatatc ttgagtatat
      781 ggactttttt gtgaaagaat ttgatacaaa tttatatgtt gctggtgtta ttcctttcat
      841 gttggaaagc ggtgacgcag tagatcagga aatgtataaa caagcccaag agatttatgg
      901 ggaacatttg attaaaaatg ttgtactcaa aaatgcgcgc ttaaaacgat acgacggatc
      961 aggtattacg atggagaaga caaagaaagg tctcttaaaa caatgggatc gcaaaagcca
     1021 cgaattattt atcagtattt tgaaagaatt ggaagaacac gagaattggt atgcggaggt
     1081 ggagtaattg gctgacatca aaggacttga tatttttggg aacaacaaag gactcgccaa
     1141 aataaaaaaa gaagcagaag caaagaaaaa gcagcaagca attacaccat ctgaaaataa
     1201 gcaattagaa gtgcaccaag ttcaagaaat aaaaacttcc tccaatgttg aaagacagag
     1261 tagaggaaga acaaaa
//
LOCUS       DAXWOO010000309         1263 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.316, whole genome shotgun sequence.
ACCESSION   DAXWOO010000309 DAXWOO010000000
VERSION     DAXWOO010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.316"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            64..1242
                     /locus_tag="ACGE70_002946"
     CDS             64..1242
                     /locus_tag="ACGE70_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
                     /protein_id="HFF8694497.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKVRTLNDKYVAI
                     FMDATYIPLKRQTVSKEAIYIAIGIREDGAKEVLSYAIAPTESTYVWNELLQDIYSRG
                     VHDVLLFITDGLKGMKDTIHQIYPKAKYQHCCVHVSRNIAHKVRVKDRKEICDDFKAV
                     YQASSKEEANTFLGSMIEKWQKTYPKVTQSLIKNQDLLTFYEFPPGIRRSIYSTNLIE
                     SFNKQIKKYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLASMFTE
                     "
BASE COUNT          423 a          251 c          246 g          343 t
ORIGIN      
        1 aaaggaagtc ccttctgtag aatagagtta ccacaacaca ttcacagaaa agaggacttc
       61 catatgaacg attttactac agaaattcta aagactctag cgaacaaagg cgatttgaat
      121 gaattattcc gtgtccattt ggaaaaagct gtcaatacgc ttctcaaaac ggagttaacg
      181 gctttcctcg attacgaaaa gtacgatcgt attggtttta acacgggtaa ttctcgtaac
      241 ggctcctatg accgtacggt taagaccgag tacggggaac ttcatctcca gattccgcgc
      301 gaccgcaacg gcgagttcaa gcaacagact gttcctgctt atagacggac gaatgatacc
      361 ttagaggaga ccgtcattca cctcttccga aaaggtatta ccatgtcgga aatcgcagac
      421 ttgattgaga aaatgtatgg gcatcactac acgccccaaa ccatgtccaa catgactaaa
      481 gtgctgactg aagaagtaaa tgcatttaaa gttagaactc taaatgataa gtatgtcgct
      541 attttcatgg atgctactta tattccttta aaacggcaaa ccgtctccaa agaagcaatc
      601 tatattgcca ttggtatacg agaagacggc gctaaagaag tactaagtta tgcgattgct
      661 ccgactgaat cgacctacgt ttggaatgag ttgttacagg acatttactc cagaggggtt
      721 catgatgtct tactgtttat taccgatggc ttaaaaggca tgaaagatac gattcaccag
      781 atttatccta aagctaaata ccaacattgc tgtgttcacg tttcccgtaa tattgctcat
      841 aaagtacgtg tcaaagatcg aaaagaaatc tgtgatgatt ttaaggctgt ttatcaagct
      901 agctcaaagg aagaggcaaa taccttttta gggagtatga ttgagaagtg gcagaaaact
      961 tatcctaaag tgacgcagtc actgataaaa aatcaagatt tattgacttt ttatgagttt
     1021 ccgcctggta tccgccgaag catttactca actaatttaa tcgaatcttt caataaacaa
     1081 atcaagaaat acagccgcag aaaagagcag tttcaaaatg aagaatcact agagcgtttc
     1141 ctagtatcca tctttgatac atacaatcaa aaattcttaa atagaagcca taaaggcttc
     1201 caacaagtga cggatacatt agcttcaatg tttactgagt aacccattat tttgcagaag
     1261 gac
//
LOCUS       DAXWOO010000310         1251 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.317, whole genome shotgun sequence.
ACCESSION   DAXWOO010000310 DAXWOO010000000
VERSION     DAXWOO010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.317"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            74..697
                     /locus_tag="ACGE70_002947"
     CDS             74..697
                     /locus_tag="ACGE70_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFF8694498.1"
                     /translation="MKIAYIRVSSIDQNEQRQIEEMKKFGAEKIFIEKQSGATITHRP
                     IFQEALEFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PHLDKFIKDLIIQILAMIAEQERTESKRRQAQVIKIAKANGVYKGRPKLYSADTKNPQ
                     RRLVYENIVKDLNEGIAISKIAKNYNITRQTIYRIKNDIEYNSNQMN"
     gene            complement(694..>1251)
                     /locus_tag="ACGE70_002948"
     CDS             complement(694..>1251)
                     /locus_tag="ACGE70_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694499.1"
                     /translation="LVDNLDNLLPDKLIYDENEDNSQKQIIIFELYLKWLSSLSVSPE
                     SNDDDKYFANKLFQFIFPDYSSMPFFTLINFMLYVKEHLGYDDLVEMIKAYDKEPYPF
                     KGINTGSDMAMISGETVEEILANYKEQNIKTLELIKKNIKYYDPLLANKENIDKLLNA
                     IADSDVRSPRIDELKEEILMYKELF"
BASE COUNT          448 a          194 c          191 g          418 t
ORIGIN      
        1 ctttatagtg tataaaaaaa ttagtgttga ttttgagtgt attttaaata gtttgaggaa
       61 gtaggtaaaa attttgaaaa ttgcttatat tcgagtttcg tcgattgatc aaaatgagca
      121 acgccaaatt gaagaaatga aaaagttcgg cgcagaaaaa attttcatcg agaaacaatc
      181 tggagcaacg attactcatc gaccgatttt ccaggaagct ttagagtttg tccgagacca
      241 agatattttt attgtcgaag caatcgaccg acttggaaga aattacgacg aaatcattgc
      301 ttcagtcaat tacctaaaga agaaaaacgt gcagctgatc atcaccagtc taccgatcat
      361 ggctgaagca attggcaatc ctcatttgga taaattcatt aaagatttaa tcatccaaat
      421 tttggccatg attgcagagc aagagcgtac cgaatctaaa cgacgccagg cgcaagtcat
      481 taaaattgca aaagcaaatg gcgtctataa aggtcgtcca aaactctata gtgcagatac
      541 aaaaaatcct caacgacgtt tagtttatga aaacatcgtt aaggatttaa atgagggcat
      601 agctatttca aaaattgcta aaaactacaa tattactaga caaactattt acagaattaa
      661 gaatgatatt gagtataaca gtaatcaaat gaattaaaaa agttctttat acattaaaat
      721 ttcttctttt aactcatcaa ttctaggaga tctaacatca gaatcagcaa ttgcatttaa
      781 taacttatca atattttctt tattcgctag taaaggatca tagtatttta tattcttttt
      841 aattaattcc agagttttta tattttgttc tttgtaatta gctagaattt cttctacagt
      901 ttcgcctgaa atcatagcca tatctgaacc agtattgatt cctttgaatg gataaggttc
      961 tttatcatat gcctttatca tttctactaa atcatcatat cctaagtgtt ctttaacata
     1021 cagcataaaa tttattaatg taaaaaatgg cattgaagaa tagtctggaa atataaattg
     1081 aaacagtttg ttcgcaaaat atttatcatc atcattgctt tctggtgaca ctgacaggct
     1141 gctaagccac tttaaataca attcaaatat aattatttgt ttttgtgaat tatcttcatt
     1201 ctcatcataa ataagtttat cgggtaaaag attatctaag ttatctacta a
//
LOCUS       DAXWOO010000311         1242 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.318, whole genome shotgun sequence.
ACCESSION   DAXWOO010000311 DAXWOO010000000
VERSION     DAXWOO010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.318"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            92..1132
                     /locus_tag="ACGE70_002949"
     CDS             92..1132
                     /locus_tag="ACGE70_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFF8694500.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
BASE COUNT          505 a          178 c          202 g          357 t
ORIGIN      
        1 atgcgtttgc attttttatt tatctttgct attttcgaaa agagtaggta attttacata
       61 aataaaacaa cgtgaaagta gaggatctac aatgactgat tttaaatttt ttaaggcgga
      121 tagagtttac aacgaattat tttatcaatt tccaaaagtc tttattgttt ctgacgaata
      181 caaaaaaatg aaagattcaa ctaagattgc ctatatgctt ttaaaagcaa gattagagat
      241 cgcaatcagc aaacggcaaa tcgatgaaga aggtaatgtt tattttactt atacgacaaa
      301 tgaactatgt agagtattaa actgccaaaa acaaaaagcg atagcaatca aaaaagagtt
      361 ggaatccttt ggtttattat tacaaaagca gatgggattt aacaaacagt tagggaaaaa
      421 taatcctaat agactatatc tagcagaatt aaaagtctca gaaaatgata tctacttact
      481 cgaaaaattt gatagagaga atagggaaaa cgttgataaa tcagagggta tgaaaatcat
      541 acccaccctc gacgaaaaat cagacgctga atcccttggg gctcaagagg gtatgaaaat
      601 cataccgtgc caaaacgttg ataaatcaga gggtatgaaa atcataccag aacttaataa
      661 taatatatta gacactaata gacacaatat agacactgaa aaagaccgcc tacaagatca
      721 attgttgtta gacaattttg agacaattat gacaaacgac agcattgcta cgtttgtccc
      781 tgaacgagta ttaaatttga taaaaacatt ttcttcaagt tacagtgaag ctcaaaaaac
      841 cgtccagact attcataatg caaagaaaaa agctgaaata gaaagtgata tttcgatagt
      901 ttttgaagaa ctcgatagtt attatgtcaa tgcagaacaa gaattataca cgacactgtt
      961 aaaagcctat caaaaattaa aaaccgaaaa agtcgaaaat atccagaacc tgatttttgt
     1021 ctatgtaaaa aattggttta tcgaaaaacc aatagctgct aaagtatcaa gtgaaaaacg
     1081 tttgaattat gcaagctccc caagcactat tacgaaagac tggttagagt gaaactttac
     1141 tgacaaagtg attgttgctt catggagtaa cattaaaatt aaaaattttt tattattcta
     1201 attatattac gaatattaca aaataatagc tattttaata ga
//
LOCUS       DAXWOO010000312         1163 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.319, whole genome shotgun sequence.
ACCESSION   DAXWOO010000312 DAXWOO010000000
VERSION     DAXWOO010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.319"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(<1..109)
                     /locus_tag="ACGE70_002950"
     CDS             complement(<1..109)
                     /locus_tag="ACGE70_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="similar to full-length Gnd, these proteins seems to
                     have a truncated C-terminal 6PGD domainin; in
                     Methylobacillus flagellatus this gene is essential for
                     NAD+-dependent oxidation of 6-phosphogluconate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase
                     (decarboxylating)"
                     /protein_id="HFF8694501.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVK"
     gene            108..956
                     /locus_tag="ACGE70_002951"
     CDS             108..956
                     /locus_tag="ACGE70_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFF8694502.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
BASE COUNT          398 a          203 c          197 g          365 t
ORIGIN      
        1 attttacaaa aatatttttt gtttgatttt ttcctactct ccttgtatct ttaacattaa
       61 tgagaacgga aaaaatgata atcttattat gaaaatgagg aattctcatg agcgtctcca
      121 taagactaca aatcaaaaca atgtacaacg acttcagccc aaaagaacag gctatcgccg
      181 attatatttt agaaaatcca agcaaagtaa gtcatagtcc tattagcaat ttagcaagtg
      241 aactgggcat tgctgactct actttttttc aatttacaaa aaaacttggt tataatggat
      301 ttaaagattt taaaatggcc atgttgatcc aagaaaatga tctatctgct atttctatcc
      361 acgaaaatat tcagaaaaca gatactgagc tcactatggc gcaaaaggtg ttcgagtcta
      421 gtatttcaac tttaaatgac acaaaaaagt tgttacaaca aaaggatcta aaaaaagcgg
      481 ctactattat caatgaatct aaacgtgtct attttttcgg tgttggcggt tcagagattg
      541 tagcaactga cgcttatcat aaatttttgc gttcccctat cgcaacttct cacagcacgg
      601 attaccatat tcaattgatg gaagcttctt tattgacgga aaaagactgt gctgttttga
      661 tttctcacac agggcaatca aaagaaacaa tccatattgc tgaaaccgta agaaaaactg
      721 gagcaaaaac aatcgttatt actagccaag ctaattcttc ccttgctaaa cttggcgatg
      781 tcgtttttat ttctatttct gaagaaacag aattccgatc tgaagcttta gcttcccgta
      841 tttctcaact aagcattttg gactctctat atgtgatttt gatgttttac aacgaaaaag
      901 aatcacgtcg ttctatcgct aaagttcgac atactatttc tgaaatcaaa gaatagcacc
      961 ataacagaaa aagcgaaaaa tccataaaac aaaaaaagcc actaccaaat atttggtagt
     1021 ggcttttttg gttctgcaag ggtggtacag aacatcaata gtcggtgggt tagaacgtat
     1081 gtcatcgcat ctttggagaa ggttgtattg gtattattcg agttacttgt tagatgcgat
     1141 aacaaagtta gtatacagta gta
//
LOCUS       DAXWOO010000313         1150 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.320, whole genome shotgun sequence.
ACCESSION   DAXWOO010000313 DAXWOO010000000
VERSION     DAXWOO010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.320"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            29..403
                     /locus_tag="ACGE70_002952"
     CDS             29..403
                     /locus_tag="ACGE70_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFF8694503.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(517..1146)
                     /locus_tag="ACGE70_002953"
     CDS             complement(517..1146)
                     /locus_tag="ACGE70_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFF8694504.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
BASE COUNT          394 a          162 c          211 g          383 t
ORIGIN      
        1 taagaaaaag aaagagggcg tattagttat gtatcttaaa attacggaac aagcacaaga
       61 aagattacaa aaatatattg atgaaggcgc aacagttata ttggatttag atgatggcgt
      121 tggggaatat tctaaaatgg gtgtttgctc tttagatact agttttcgtt tacttcttct
      181 tgataaagaa cagagaaaaa atgattataa actccaatta gatagtgata ttggggatgt
      241 ttatataaaa gattattcaa aaatgtacat ggatgaacat atgacccttt ctttagatcc
      301 acgtctaggt gtatttactc ttgaaagccc aagtggttct ttagacagtc atgttcaatt
      361 agtagatcta cgaccagaaa aattctctgg aataaaacaa taactttctc tctattgtcc
      421 ataaaactag tctcaatcaa gctgtattga aaaaacaaga caacattctt accatggtaa
      481 ataaaaagac gacacatttt gtcgtctttt tttaaattaa ctattttcga taaaagatcg
      541 catagcttgt gccattattt ctggcttttc tctattcaat tcatgttttc cgtttttaat
      601 aaagagtagc cggccattcg ttgaaagttt atataaacgt tttgctgctg gtttattcgc
      661 atgatctttg gtaccgcata aaatcaattt aggaagtcgt gatgtacgaa gctgtttttt
      721 gcagtctaat gtttcaagtg aacgaagtag ggtaagggtt tgttttttag atagacctaa
      781 tttttcaaat atcttagaag gtacgcagta aaaataaata ttctggaaag taagcaataa
      841 gcgattagga gactcaaatt gtggagcgac taaaacaacc ttttttaaat atggagaagg
      901 agttgcaaga tagtttaaag caataacagc gcctaaagat aatccacata aaataaaagg
      961 ttcctttatc tgattcaatt gtacttcgat gtatttggtt aagtcgtcta aagtagcagt
     1021 gtctgtgctg tttaaatgat tgaacaatga aagacttttg caagaataac tattgggtag
     1081 ataagagatt acatctttcc atgattgttc gtcttggcct agaccatgca aaaaaacaat
     1141 tttcatattg
//
LOCUS       DAXWOO010000314         1137 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.321, whole genome shotgun sequence.
ACCESSION   DAXWOO010000314 DAXWOO010000000
VERSION     DAXWOO010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.321"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..355
                     /locus_tag="ACGE70_002954"
     CDS             <1..355
                     /locus_tag="ACGE70_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694505.1"
                     /translation="GAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETNMTRDELL
                     RKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTTSQIKQDT
                     EDSLRKGWGMNNKVR"
     gene            615..910
                     /locus_tag="ACGE70_002955"
                     /pseudo
     CDS             615..910
                     /locus_tag="ACGE70_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            985..>1137
                     /locus_tag="ACGE70_002956"
     CDS             985..>1137
                     /locus_tag="ACGE70_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFF8694506.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWV"
BASE COUNT          431 a          135 c          237 g          334 t
ORIGIN      
        1 tggcgcacca atcagaaagt ttgataaggt ttattccacc aagcatcctt taaaactgtc
       61 gactttgtta aatgcgactt ccagagtttt agtagaaaag tatgagacta atatgacaag
      121 agatgaattg cttagaaaag ctttagatga gtatattaaa cagaatctta cgcaagaaga
      181 taagcaagat ttgtttaatg acgtggtgaa agaactagac ttgttcagag aaaaatatcc
      241 aattgttcca gaattagatg atcgtggaga aataataaga actaccagtc agataaaaca
      301 agataccgaa gactcactgc gcaaagggtg gggaatgaat aataaagtta gataataatc
      361 aaagaagaac taagtaacta cctttaaacg gtagttactt agttcttctt tgatttgcat
      421 gaaatagagg ggatttattt ttctggttac gtgattaaaa aatccaaaaa atgagcatag
      481 gaacggttat gattttaggg tgtgttttaa tgagatattg tgatttcatt aacaagtgta
      541 tgataatctg ttgttgagtt agtaaataag taatagagct aactctaaaa taaaactatt
      601 aggaggagat gtaggtgaaa aagtttaaaa agaaaatata tttaggattg gttgttgggg
      661 tgagtatgtt tgggttcatg gctcatacta aacaagtaac tgcagatact aaccaaaaag
      721 aaaggattat gagccaatta gatgactctc aaataagtat gtacccaatg agctaattgt
      781 cagtttaaag gctacacaaa gaatttcaac agagaaaaat aaaccgcaaa atttattggg
      841 tgtaaaagtt atggtggaaa aagatttgac tcttaggaca aatgaaagcg attgtaataa
      901 gcgagatcaa gggttctgtt gcaaagtttt ccaaaaaatc tattttagtg taaaattgag
      961 aaaaaagaca gagaggacag agtaatgaat cattttaaag gcaaacaatt caaaaaagac
     1021 gtcattattg tcgctgttgg ttactacctg cgttacaatc taagctatcg tgaagttcag
     1081 gaattgttat atgatcgtgg aataaatgtt tgtcatacta cgatttatcg ttgggta
//
LOCUS       DAXWOO010000315         1134 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.322, whole genome shotgun sequence.
ACCESSION   DAXWOO010000315 DAXWOO010000000
VERSION     DAXWOO010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.322"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            4..>1134
                     /locus_tag="ACGE70_002957"
     CDS             4..>1134
                     /locus_tag="ACGE70_002957"
                     /inference="COORDINATES: protein motif:HMM:TIGR02331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFF8694507.1"
                     /translation="MPEGTKVNDVTPEGVIDTTQPGEYEGTIEVTYPDGTKDTVKIPV
                     EVVDNRSDAEKYTPEVEKEEVNYGGTIDLTDNVTNLEELPEGTKVNDVTPEGVIDTTQ
                     PGEYEGTIEVTYPDGTKDTVKVPVEVVDNRNDAEKYTPEVEKEEVNYGGTIDLTDNVT
                     NLEELPEGTKVNDVTPEGVIDTTQPGEYEGTIEVTFPDGSSTTVKVPVTVKDKVDWVE
                     INPIPTIDPEQIVPEEVHKGEEADLTDNILNLPEGSKVEVVTPVDTTQVGEHTGEVKV
                     TFPDGSSVVVKIPIIVKNMDDMITNNQMTRVEKNSVVLDVINKSKSLSSSKIFGKSDN
                     MYVETSNKELPKTGEENTTILSILGMLSISFCVLLFRLFKKRE"
BASE COUNT          447 a          173 c          263 g          251 t
ORIGIN      
        1 gagttgccag aaggcacaaa agtgaacgat gttacaccag aaggtgtcat tgatacgact
       61 caaccaggtg agtatgaagg aacgatagaa gttacgtacc cagatggaac aaaagacaca
      121 gtaaaaattc cagtggaggt agtggacaat cgaagtgatg ctgaaaaata tactccagaa
      181 gtagaaaaag aagaagtcaa ctatggagga acgattgatc tgacggataa tgtaactaac
      241 ttggaagagt tgccggaagg cacgaaagtg aacgatgtta caccagaagg tgtcattgat
      301 acaacccagc caggtgagta tgaaggaacg atagaagtta cgtatccaga tggaacaaaa
      361 gatacagtga aagttccagt ggaggtagtg gacaatcgaa atgatgctga aaaatatact
      421 ccagaagtag aaaaagaaga agtcaactat ggaggaacga ttgatctgac agataatgta
      481 actaatctgg aagagttgcc ggaaggcacg aaagtgaacg atgttacacc agaaggtgtc
      541 attgatacaa cccagccagg tgagtatgaa ggaacgatag aagttacctt cccagatgga
      601 tcaagtacaa cggttaaggt gccagtaaca gtcaaagata aagtagattg ggtagaaatc
      661 aatccaattc caacaatcga tccagaacaa atcgtgccgg aagaagttca taaaggagaa
      721 gaagctgacc taacagataa cattctcaac cttccagaag gaagtaaagt ggaagtcgtc
      781 acaccagtgg atactacaca agtaggcgaa cacacaggcg aagtgaaagt taccttccca
      841 gatggttcaa gtgtggtagt aaaaatccca ataattgtga aaaatatgga tgatatgata
      901 actaataatc aaatgacgag agtagaaaaa aattctgtag tactcgacgt tataaataaa
      961 agtaaaagct tatcttcatc gaagattttt gggaagtcag ataatatgta tgtagagaca
     1021 agcaataaag aacttcctaa aacaggagaa gaaaatacta caattttatc tattttaggg
     1081 atgttatcaa ttagtttctg tgttctttta tttagattat tcaaaaaaag agaa
//
LOCUS       DAXWOO010000316         1105 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.324, whole genome shotgun sequence.
ACCESSION   DAXWOO010000316 DAXWOO010000000
VERSION     DAXWOO010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.324"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            3..1085
                     /locus_tag="ACGE70_002958"
     CDS             3..1085
                     /locus_tag="ACGE70_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015076493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFF8694508.1"
                     /translation="MNDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTDT
                     RVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINAE
                     FKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKIIS
                     LEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVKN
                     DGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKEDG
                     ELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISIT
                     MDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT          402 a          193 c          213 g          297 t
ORIGIN      
        1 atatcaatga tgatggctca agaaaaaaag tggagaaagt tggcggagac acccgaccag
       61 aggccgaagc tgctttacga aaagttttat cagatattga cgaaacagga caatactttt
      121 taggtacgga tactcgagta aaacaatacc ttgatttttg gatggaggaa tacgttaaac
      181 taaatctaaa atacaatacc tatgaaaact accgatttac catcaaaaat catataaacg
      241 gttatttagg aaagaaaaaa cttacggatc tctcccctgc tcttttacaa aatttcatca
      301 atgctgaatt taaaaagggt tactcgaaga aaacaatgac tattactcac tctgtcctta
      361 agaatgcgct gaatatggcg gtttatcctt ggggattaat caagcaaaat cctatgctgt
      421 atgtaaaaat accaaaatat gaagcacgac caacgactaa aaaagatcta aaaatcattt
      481 ctcttgagga ctttgatcat atgctagaaa ttactcctga aggacatcct ttctatattc
      541 ctttgaatat tggattttat acgggaatgc gcgttggcga agtttgtggt ctgacgtggg
      601 atgatgtcga tttttcaaat ggaacaatta ctgtagagaa acaaatggta aagaatgacg
      661 gcgcatgggt atatggtacg ccaaagacaa gcagttccaa tcgaacgatt tttattggac
      721 aaaccttgct agcaattctg aaaaaacata agaaacaaca attagaaaat cgaatgaagt
      781 atggaaagct ctacattgat tcaaatgcag tatgtacgaa ggaagacggt gagctagtta
      841 cgccaagtgt agtgaaatgg aatactagga ggatatcgaa tgcactctcc ctctctttta
      901 acttccattc tctcagacat actcatgcta cacttcttct cgaaaatggc gcaaaaatga
      961 aagaaatctc tgaacgattg ggccacagca gaatttcaat tacgatggat acttactcgc
     1021 atgtaacaga taagatgaga aatgaaacgg tcgatatcat ggaaaatctt agaaagaatt
     1081 cttgattttt gccaccgaaa aaaac
//
LOCUS       DAXWOO010000317         1094 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.325, whole genome shotgun sequence.
ACCESSION   DAXWOO010000317 DAXWOO010000000
VERSION     DAXWOO010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.325"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(<1..977)
                     /locus_tag="ACGE70_002959"
     CDS             complement(<1..977)
                     /locus_tag="ACGE70_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8694509.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFY"
BASE COUNT          354 a          134 c          191 g          415 t
ORIGIN      
        1 aaataaaaat aaattggtat tttattttct aaatggttga gaggaaactg ttgagttaag
       61 taagtcatca attgaccgac ttgtcgacaa ataaggtttt tgcttgtgga aaataaaaaa
      121 ccaatctcct gtttttgtcg aaaagagatt tgatttggaa aaaaacaata gaaaaaataa
      181 ttaaagtagg ctctctctgt ttctgtctct tcacaagggc gactcactga aaaagaagta
      241 aagtattctg aataatcatg tgtatgctgc aataagtcca tcaattcagt gatttcagaa
      301 gaagtttttg gaaaatatgc ctgttgtttg tttcgtaata tgaataaagt taacagcaag
      361 tagatatttt tttcttgttt tgaaattttt tctatttgct catctgtact gttcattttt
      421 agtgcattta attcaagtga ggaaaatggc caatcgttac ctccaaatgt atctgaaaga
      481 aaaaagtaaa gatagagacg gatagataac tcatttcctt caagagtaca gcaatttttt
      541 acagatagga tccgtaaatg gaattttttt aaggaatgat tgagctgttt tgtcagtttt
      601 tcgagatagg agggcgatat ataaagttgt tcaagtaaat atttttttgg tacagcagtt
      661 tgtggataat tgattagtaa catcaaatag ttaaaacgat tggattcttt taggtagcat
      721 aattttaact tagaaaaaac agctaacttt ttagtttgat cggtttcttt ggtttggatc
      781 tctcctttat gattagaata aataaattca tcaatcaata aaaattgctt gagtgataca
      841 tttatttttc ggatcagttc tttgattgtc cgttttggaa tatgtaagtc attggataat
      901 tcttgtaaag aacaattatt tttttgaatg accatttgta gaacaagtaa ctcttttttt
      961 cttgcttcaa ttagcataaa tacctccttt ttattctgtt tttgtatata gttagacaaa
     1021 aactgtgagt ggaaagtcat ttcagtctgt gttattctca cagagtgtcg ccttcttttt
     1081 tatataacgt tatt
//
LOCUS       DAXWOO010000318         1073 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.327, whole genome shotgun sequence.
ACCESSION   DAXWOO010000318 DAXWOO010000000
VERSION     DAXWOO010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.327"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..>1073
                     /locus_tag="ACGE70_002960"
     rRNA            <1..>1073
                     /locus_tag="ACGE70_002960"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          329 a          214 c          308 g          222 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact aggatgttgg cttagaagca
      961 gccaccattt aaagagtgcg taatagctca ctagtcgagt gaccctgcgc cgaaaatgta
     1021 ccggggctaa acatattacc gaagctgtgg agtacacctt taggtgtatt ggt
//
LOCUS       DAXWOO010000319         1070 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.329, whole genome shotgun sequence.
ACCESSION   DAXWOO010000319 DAXWOO010000000
VERSION     DAXWOO010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.329"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(58..933)
                     /locus_tag="ACGE70_002961"
     CDS             complement(58..933)
                     /locus_tag="ACGE70_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFF8694510.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
BASE COUNT          331 a          209 c          151 g          379 t
ORIGIN      
        1 catagtccat ttactatgtg tcatttggtt gcgcctatag atagttacgc ccttatttta
       61 atagattctt gctagatgaa ttgccaaata gatgatttca ttttcttgaa tggcaagttg
      121 aaattgctgt tcgatgatat ctttaatctt tagtgctgta caatacgctt tgctatgttt
      181 aaatgtttca gtaaaaaaac caatattttc tactggcgtt tcttcctgat aaatcaagcg
      241 tttaaccagg aatttgatat gaaccattaa gcgatgataa gtcagtgatt ctttatcata
      301 aacgtttgaa taggattttt ctagatggga caaaatcgtg gtcgtcattt ctactatttt
      361 taagctcttt aaactattag gctctcctag ctcagcatta atgatatgca tagtgatgaa
      421 tcctacttca tttgacccta actcggtttg atatcgatga ttgattcgct cgaccacttc
      481 tactgctata tcatattcat ttggataaag gaatctcatt tctggcaaca taattgctgt
      541 gacatctcta tcttctcttt ttcgttgaat agcaaaataa atatgatctg tcaatgtaat
      601 atagatattt gaatctaatg gagaggagat cctagctgtt atcagttcaa tcacttcttc
      661 cgcaaaatcc ataaattcga ttggaatctg cgagactatc tcactgaatc gtttgatctt
      721 ttctttagaa tctaatacaa ataatttgtc agcatcctca acatcaaatg gcttactcac
      781 tttagcagaa aagccaatcc ctttccccat cgcaattttt tctattccat cgtcatcgat
      841 caatacagca ttatgattca gctttttcat cacatacacc gttaattcac ctcctctata
      901 tgataaaaca aaacaacttg cagactcctt catacatatt tttgtctcca aacaattttt
      961 aaagacaaaa aacgacctaa gaaagaaccc tttcttaggt cgtgcctaat tgaatagtag
     1021 caccctacat aatttacaaa aatagtttaa cacataataa aagcgttatc
//
LOCUS       DAXWOO010000320         1052 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.330, whole genome shotgun sequence.
ACCESSION   DAXWOO010000320 DAXWOO010000000
VERSION     DAXWOO010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.330"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            109..963
                     /locus_tag="ACGE70_002962"
                     /pseudo
     CDS             109..963
                     /locus_tag="ACGE70_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT          376 a          165 c          202 g          309 t
ORIGIN      
        1 aaaagatagc gtaaaatatt ttgtgtaaat agaaatttag ggtagaaaaa aaagtccatt
       61 ctgtaaaatt aaagttacca cacagaaatt ttataggagg actttctcat gactaaattt
      121 actatagaaa ttatgcaaac actactcaat aaaggggatt tagatgaatt atttcaggat
      181 catttagagc gagcgattaa ttcacttcta caagcagagc taactgcttt tttagattac
      241 gaaaaatatg atcgaaatgg attcaattca ggaaattccc gtaacgggaa ttattcacgt
      301 acatttaaaa cagaatatgg cgagttgaat ttaactattc ctagagatcg gaatggcgaa
      361 ttttctcaac aaacactacc agcatataag cgaaccaatg attcattaga aacgaccatt
      421 atccaattat ttcaaaaagg cattactatg gctgaaatct ccaaattaat tgaaaaaatg
      481 tatggtcacc actatacgcc acaaacaatc tccaacatga gtaaattggt ggctgaagat
      541 gttttagctt ttaaagaaag aacgttagaa gccaattatt ccgttatatt tatggatgcg
      601 acgcatatcc ctgtgaagcg acagactgtt tcgaaggaag cagtgtatat tacgattggt
      661 attcgtttgg atggaaccaa agaagttctt gggtttacta ttgccccaac tgagtctgct
      721 tatatttgga aagaagttct tcaagatctt agaaaacgtg ggttagaaga agttttatta
      781 gtagtgacag acggattaag ctgtattgaa gaaagtatcc atagtgtgta tccgaatgcc
      841 caatttcaac aatgttgtgt gcatgtatct agaaatatcg ctcataaagt tcgtgttcga
      901 ggtcgaaaag aaatttgtga ggatttcaaa ttggtttacc aagcgaattc aaaagaagag
      961 gcgtaagcgc cccatagaac agtaccttac taacaaatgc acctcaagat atacttgagc
     1021 tatcaagaag tctggaggtt ttctatgtat aa
//
LOCUS       DAXWOO010000321         1027 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.331, whole genome shotgun sequence.
ACCESSION   DAXWOO010000321 DAXWOO010000000
VERSION     DAXWOO010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.331"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(55..975)
                     /locus_tag="ACGE70_002963"
     CDS             complement(55..975)
                     /locus_tag="ACGE70_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFF8694511.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT          318 a          212 c          190 g          307 t
ORIGIN      
        1 tttacttttg tcccacgctc tttttagtga gattcgttgc atcgtttgtc caaattattt
       61 tccgacagca attggactgt tcggaatcgt gaaaccagaa gctgtcaatt ctttgacata
      121 ggagctcaag aactcttctt ttaaggcata ttgttttccg ttcaatgcgt agcagaccgt
      181 acgaatcgca aaattgctgt ttccaagatc cactaatcca aagatagaag gttcagtttg
      241 gagctcttct tggtattttt cagctaatgt acgatttact cgatcaatga tctcatagat
      301 tttgtcatat ccttcttcag gaacaatccg aatatctaaa agtacttgca tatttgcacg
      361 agagagattg ctgatcgtgg tgatgtttcg attcgggatg aaatggacag taccatccgt
      421 tgctttcagc tgaagggtac ggataccaac agatacgacg gttccttcaa tcgttaagtt
      481 tgctagcttg atgtagtccc caacatccat ctgttgttcc atgataataa aaaatccggt
      541 aataacatca ctcatgaacc cttgggcacc aagaccgatt gccacacctg caattccggc
      601 accagccaaa agtgatccga tcggcacacc gatagcagat aaaattgcat agataaaaaa
      661 gaaaccaagg gtataatgaa aaatagtatt taaaagacta tgtaaagtct ttaagcggct
      721 ctcgtttaga atggcttttt tagaatactg attaaaagac ttatcaatca gatattttcc
      781 cacacggtga agaatccaaa aaaggacgat caaaaaaatc agatataaac ttttttggat
      841 aaaggcagcc aaaatggcgt cccaatcaat cccgtcccaa aaccgctgaa acgcatttac
      901 tttgctgaca gcttgatctg tcaatgtctc gccagaagag tctgtcgtac cggcagttgc
      961 taataatgaa aacataaaat cctcttttct aaaatttgat tctttctgta ttgtaccaag
     1021 aagtttt
//
LOCUS       DAXWOO010000322          974 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.332, whole genome shotgun sequence.
ACCESSION   DAXWOO010000322 DAXWOO010000000
VERSION     DAXWOO010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.332"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..896
                     /locus_tag="ACGE70_002964"
     CDS             <1..896
                     /locus_tag="ACGE70_002964"
                     /inference="COORDINATES: protein motif:HMM:NF016640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase domain-containing protein"
                     /protein_id="HFF8694512.1"
                     /translation="KRNLEIHELNQKNNSILGMKIRNKINQLNQMKKRDNLLPAKYTL
                     KSMTGKGPIKDYSNRENRSDELRKKELTNQGTPKRIAVYSCITGNYDSIKEPDFSHPL
                     VDYYLYTDNVITSEHWNIREIPKYISELKNNILINRYIKFHPNELFTDYDFTLYVDGN
                     VTITSDITGLLNSSSKLGLSMHKHSERKSLYSEADALIAVGKGNKESIIEQISKYREE
                     GFPDNFGLAEATVILVDLNNSIAENLLDLWWREYLNSESGRDQLAFPYVLWKSGHAMK
                     EVTTLGNNVYKSAKLRIISHY"
BASE COUNT          371 a          126 c          175 g          302 t
ORIGIN      
        1 aaaaaagaaa tcttgaaata catgaattaa atcaaaaaaa taattctatt ttaggcatga
       61 aaatacgaaa taaaattaat caacttaacc aaatgaaaaa acgagataat ctgttgccag
      121 caaaatatac ccttaaatcg atgaccggta aggggcctat taaagactat tcaaacagag
      181 agaatcgcag tgatgaacta agaaaaaaag agttaactaa tcaaggaaca cctaaaagga
      241 ttgctgtgta tagttgtatc actggtaatt atgattctat taaagaaccg gatttttctc
      301 atccattggt agattactat ttgtatacag ataatgtgat tacaagtgag cattggaata
      361 ttagagagat acctaaatat atatcggaat taaaaaataa tattttgatc aatagatata
      421 ttaaatttca tcctaatgag ctatttactg actacgattt tactttgtat gttgatggta
      481 atgtaacaat aacttctgat attaccggat tactgaacag ttcttccaaa cttgggttaa
      541 gtatgcataa acatagtgaa agaaaatctt tatattctga ggcagatgca ttaattgcag
      601 ttggcaaagg taataaggaa agtattatag agcaaattag taagtacaga gaagaaggat
      661 ttcctgataa ttttggattg gctgaagcca cggttatttt agttgattta aataattcaa
      721 tcgctgagaa tcttctagat ttgtggtgga gagagtattt gaactcagaa agtgggcgag
      781 atcagctcgc atttccttac gtgttgtgga agtccggaca tgcaatgaag gaagtgacta
      841 ccttaggcaa taatgtatat aaaagcgcta aattaagaat cataagtcat tattagccct
      901 aaatgatttc taaactattc tttcaaatcc tagtaaatat ttggagatag aagatttcct
      961 ttttaaagtc ataa
//
LOCUS       DAXWOO010000323          974 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.333, whole genome shotgun sequence.
ACCESSION   DAXWOO010000323 DAXWOO010000000
VERSION     DAXWOO010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.333"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            82..>974
                     /locus_tag="ACGE70_002965"
     CDS             82..>974
                     /locus_tag="ACGE70_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFF8694513.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINH"
BASE COUNT          331 a          182 c          191 g          270 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc atta
//
LOCUS       DAXWOO010000324          972 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.334, whole genome shotgun sequence.
ACCESSION   DAXWOO010000324 DAXWOO010000000
VERSION     DAXWOO010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..972
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.334"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            73..>972
                     /locus_tag="ACGE70_002966"
     CDS             73..>972
                     /locus_tag="ACGE70_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFF8694514.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNNIPRKSLDYR"
BASE COUNT          341 a          169 c          179 g          283 t
ORIGIN      
        1 agacaactaa aaaagtcatt tgtgctacac tcaaaatagt tccgaccaaa gaattataag
       61 gagtgagtac aaatgaccta tacccatctt acaacggatg aacttgtaat aatagagtct
      121 tatttcaaaa tgaatcaatc tgttgctaaa actgcccatt gcttgaatcg ttcaagacaa
      181 acgatccata aagtatatct gttcttcaag caaggaaaat cagccttaga atattatcaa
      241 cagtataaga aaaacaaatc aaactgtggt agacgtccgc ttgttttacc cgaggaacaa
      301 tcagaatata ttcaaagaaa ggttgttcaa ggatggacac ccgatgtgat tgttggtcgt
      361 gcagcgtttc ctattagttg ttctgctcgt accatttatc gtatgttcaa aaaggggcta
      421 tttgattctt ctgacttacc gatgaaaggc aagcgtaaac cgaatggaca tcaagaaaga
      481 cgtggaaaac aaactttccg ccgctctatt catgaacgtg aaaaggatta tagccaattc
      541 tcaaatgagt ttggtcacct tgaaggtgac actatcgtag gtctgaaaca taaaagtgct
      601 gtaattacct tagttgaacg attatcaaaa gttatcatca cattgaaacc gtgtggtaga
      661 caagcgattg atattgaaaa aaaattaaat caatggtttg aatctgtacc gaaaaaccta
      721 ttcaaatcca tcacttttga ttgtggaaag gaattttcaa attggaaaca gatcagtaat
      781 gtcaatgata ttgccattta tttcgctgat ccaggaacgc cgtctcaaag aggcctaaac
      841 gagaattcta acggattgtt acgtagagat ggtttattga aatctatgga tttcaattca
      901 gtagatgaat tttttattca atctgtcgca tctaaacgaa ataatattcc tagaaaatca
      961 ctggattatc ga
//
LOCUS       DAXWOO010000325          967 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.335, whole genome shotgun sequence.
ACCESSION   DAXWOO010000325 DAXWOO010000000
VERSION     DAXWOO010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.335"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            120..935
                     /gene="thiD"
                     /locus_tag="ACGE70_002967"
     CDS             120..935
                     /gene="thiD"
                     /locus_tag="ACGE70_002967"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFF8694515.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT          347 a          154 c          219 g          247 t
ORIGIN      
        1 aatttgatta ccaagaaaag gtagtggtat atttactgag ggatactttg aaaaacatat
       61 gatagaaaca gtaacagtat tatgaaaaat ctttttataa aaatggagga atcgatttga
      121 tggaagatca gtatgtacaa gcaatgacga tcgcaggatc agatagcgat ggaagcgcag
      181 ggatgcaagc agatatgcat acattcttta caagaaatgt ttacggtgtt tctgtcatga
      241 ccgcatgtgt tgcaggtaat tcttacggaa tcggagcaag tgtgacattg ccaacagatt
      301 ttatcgacaa agaatttgaa ctgattgcga aggactttca aatacgtgca gctaaaacag
      361 gtatgctagc agattcaaag ttgattgaga cagttgtaaa aaattaccaa aaatatgatt
      421 ttggaccatt agtcgtggat cccgtaatcg taacgaaaca tggaaatctt ttactagaag
      481 aatctgcttt gcaaagcttg aaagaaaagc ttgtcccttt ggccgaagta ctgacaccta
      541 atttttatga agcagaaaag ctgactggac ggacattagc agctgaagaa gattttgtgc
      601 aagcagcaaa agaactgcaa gatatgggtg caaaaaacgt tatgatcaaa ggacatcatg
      661 ctaaaaatgc gcagcaagaa acagtcaatg actacgtatt gttcgaatca ggaaaatcat
      721 tttggttaaa tgcaccattt tatcctaccg accggatcaa cggaactggc gattcgctat
      781 ctgcatgtat tgcagctgag ttagcgaaag gaaaaacagt tgaacaagcg atccgattgg
      841 caaaagacta tgtaaaccgt gcaattcgtg aagaaataaa tgtagggcac aaatatggcc
      901 ccatcaatca ttgggcagct ggtgaattag agtaaaagaa agaagttgtg aaaaagttgc
      961 cctgcat
//
LOCUS       DAXWOO010000326          957 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.336, whole genome shotgun sequence.
ACCESSION   DAXWOO010000326 DAXWOO010000000
VERSION     DAXWOO010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.336"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..214
                     /locus_tag="ACGE70_002968"
     CDS             <1..214
                     /locus_tag="ACGE70_002968"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694516.1"
                     /translation="RINLPNKFIGGFKMSHTLLIKDTLNILDLNINIHFEENCLTKEK
                     YKGQICLIYKGTLQYTPEEYPHLPIY"
     gene            274..507
                     /locus_tag="ACGE70_002969"
                     /pseudo
     CDS             274..507
                     /locus_tag="ACGE70_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(555..674)
                     /locus_tag="ACGE70_002970"
                     /pseudo
     CDS             complement(555..674)
                     /locus_tag="ACGE70_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
BASE COUNT          347 a          194 c          127 g          289 t
ORIGIN      
        1 tagaattaat ttgcccaaca aattcatagg aggattcaag atgtctcata ctctgcttat
       61 caaagatacc ctaaatattc tagacctaaa tatcaatatc catttcgagg aaaattgttt
      121 aactaaagaa aaatacaaag gacaaatttg cttgatttac aaagggactc ttcagtacac
      181 accagaagaa tatccacatc ttccaatata ttaaaagaac cttcttgaat cattgagtga
      241 aagaaaagag attcgtcact atttgaggac tgtttaacta gagacattag cgatctgcct
      301 gaaacctata caaccacttt acgaacgttt aaaaattttc tgccacagat tcaaaatgcg
      361 cttaactgca gctattcaaa cagaccattg gaatgtttaa acaaccacat caaagtatta
      421 aaacgcaata cgtatggctt tcgcagcttt tacaatttca aactacggat catgattcgt
      481 catggcaaaa catttttaac caaataaaca gaagaatgtg ggataaatct aaaaatcagt
      541 tttgctacag actctcgagt aaacggcggg agcagaagca actccttcgg aaataagccg
      601 aaattcacaa aaatttgaaa agcaattttc gtgaatttct tcttatttct cggagttaaa
      661 cacttctgtc ccagcctctt ctgtccaaat tattctaaga tttctcttta ccaacatcat
      721 ttgacaaaga gcttcttttt tgaatactat attcgttctg tttttcatct tcaaatacga
      781 actcatccgc agaaatattt gtcacaactc catgaatctt cttttcatta ccacctatat
      841 accacaggat tcctacacag aaacaacaaa aaaatagcat aaaataggtt ttctttattt
      901 tatatccctc cttttagatt aaaagctcac agaaaacgct ttctaaaaag acaatag
//
LOCUS       DAXWOO010000327          942 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.337, whole genome shotgun sequence.
ACCESSION   DAXWOO010000327 DAXWOO010000000
VERSION     DAXWOO010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.337"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            81..491
                     /locus_tag="ACGE70_002971"
     CDS             81..491
                     /locus_tag="ACGE70_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFF8694517.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            505..861
                     /locus_tag="ACGE70_002972"
     CDS             505..861
                     /locus_tag="ACGE70_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFF8694518.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          328 a          145 c          185 g          284 t
ORIGIN      
        1 tttatatttt attagctaat aattggtatt ttaaaagaaa ttactgtaga attggattat
       61 aattaaaaag gagtgagaaa atgataaaag tatatacttc gcctagctgt acttcctgta
      121 gaaaagcaag agcatggttg caagcgaatc aacttgaatt tgaagagaag aatatttttg
      181 ccgaaccgtt aacagaaagt gagatcaaaa agatcttggc agcgactgaa ggaggagttc
      241 aagatattat ttccactcgg tcgaaaattt acgaaaagct gggcatagat tttaacgaat
      301 taactttaaa gcaaatgatc gtcttattca aagaatatcc ttcattattg agacgaccta
      361 ttttgattga tgaaaaaaga atactaatcg gatttaatga agatgagatc cggtcatttg
      421 tccctcgtga aattcgatgt gttgccagaa atctgatcta tttacaaaac aataacatct
      481 taaattcata gggagggagc cttcgtggaa gtgaaagtct atggatcagc gcactgtgta
      541 acaagtctga aggcgaaaca atggttagaa caccatcaat tgccttatca atttgttgac
      601 ttagaagaac gcgatctaag tacagaagaa gcagaagaat tatgcagttt tgaagaagtg
      661 caagcagaac agctatttgc gacttggtct gaggcttttc acaacattgc gattgacctt
      721 tcctgtgtag gaaaaaaaca actgattttt ttatgcacac aacataccaa tctcctcaga
      781 cgtcctttga ttattatcga tgaagttcta tttattggat acaatgagca gttgatggaa
      841 cagcttatct taaataaata gactttaact gttttaatca agtcactgag catttcatac
      901 agttgtaaca gtcgaacact tagcatcaag cgtgttatgt gt
//
LOCUS       DAXWOO010000328          934 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.338, whole genome shotgun sequence.
ACCESSION   DAXWOO010000328 DAXWOO010000000
VERSION     DAXWOO010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..934
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.338"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(142..366)
                     /locus_tag="ACGE70_002973"
     CDS             complement(142..366)
                     /locus_tag="ACGE70_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694519.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(386..808)
                     /locus_tag="ACGE70_002974"
     CDS             complement(386..808)
                     /locus_tag="ACGE70_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFF8694520.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
BASE COUNT          266 a          194 c          124 g          350 t
ORIGIN      
        1 aacaaaaaat tcggatttta agcaaaatcc gaacgttcca tctgattaaa tccctttatc
       61 tcaaagttca tgaacattca ctttggatta caagaatttc tttattgtgt taataaagtt
      121 tcttcattct agtctcaaaa atcaatagtg tgatttgatt ttaccatttc gtttctgttc
      181 ttctttgatg aattcttttg tatcgacagg attcagatta tttagtccgc caaaacctgt
      241 atcgaccatt tttccccagc cttttgtctt cttttttcct gatttgagat tctttgctcg
      301 aagcttctca ctatgttttg ctcgccgata cttcaaataa ccttctaatc ttcccattag
      361 atccactgtt tttcctcctt tttattcaat cagacgactc agcttttcct ataacaaaat
      421 aattgccttc agggtcagaa aaattgaaag tcaagatgcc attgttatct gtgatttctc
      481 ctggttcttc catgttctcg tagatagttt caaattcatc cgtaaaaaat actaaagaag
      541 gcggtggtcc taatacctct ggtgaaaact tctgcacaaa tacttttggg aatacgccta
      601 attctaattc ttttgagatg ctgagtacaa tactcttaaa ttcttctggc aattcgattg
      661 tttcagaaat ttctgcacca attttttcta caaagaatcg gcaaatcatc tctacgtcat
      721 tgacataaag catcacacgt gttttttgta tcatcatttg acccttcttt ctatttgatt
      781 ttctttcctg tctataaact gattccatta tatcacgaaa gcgcaaacac cttgtgtttg
      841 atactattca agaaaaataa agcaatagtt tctaggattg ctttcctcca caaacttttt
      901 ttacaaaaaa aaaacggcaa tgaatgccgc tctt
//
LOCUS       DAXWOO010000329          919 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.339, whole genome shotgun sequence.
ACCESSION   DAXWOO010000329 DAXWOO010000000
VERSION     DAXWOO010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.339"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(<1..298)
                     /locus_tag="ACGE70_002975"
     CDS             complement(<1..298)
                     /locus_tag="ACGE70_002975"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8694521.1"
                     /translation="MKLLFIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGN"
     gene            complement(282..919)
                     /locus_tag="ACGE70_002976"
                     /pseudo
     CDS             complement(282..919)
                     /locus_tag="ACGE70_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
BASE COUNT          331 a          149 c           72 g          367 t
ORIGIN      
        1 gattaccttt atcagttatt aaaaacattt cagaacaacc aatctcttta gaatattcct
       61 taataaaaga taataatttt gaaccataac ctttgtcttg atagttaggt aacattccta
      121 ttgagtgtaa ataaaacatt gtttttccat caggtcttaa aagtgtatag caatatgcaa
      181 atcctataat tttattattt tctttagcta taaacccaaa tgaactggta tcattaagaa
      241 atccttttaa attatcaata tcaaaaacca tattatcatc aataaacaat agtttcaata
      301 ggataatatt cttcaactat atcttgatat tcttttgctt tctcaatatc tatatttcca
      361 tacattctta atatatcttc tccaaaattt gttcctattt cttcttcact atcttcaagt
      421 aagtatataa aatcacaata ttcatctata attccagaat ctccaaaatc aattattcca
      481 gttaatctat tattgccatc taacaataga tgattacaac taaaatcatt atggcataaa
      541 cactttttac cctcaaaaac tgttgttgca tttagtcttt ccataaaact ttctatataa
      601 tctttttcta tatcagttaa atcattataa atagtttcac gcaacaatat atactcttct
      661 aatacatttt gtttattatc aatagtacat tcactaatat ctgtataatc taaaccgtgc
      721 atttgtctta aaaaactggc aatatctcgt tttaacaaat tttgttcttc ttctgacata
      781 gtagaataaa tttctggtgt taaaaaagtt cctttaattt ctttataacc tagtatagat
      841 aattcatcac taatatacga atattcaata ttaggaattt ttacattagt ttctaaattt
      901 gtatttaaaa aattatata
//
LOCUS       DAXWOO010000330          903 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.340, whole genome shotgun sequence.
ACCESSION   DAXWOO010000330 DAXWOO010000000
VERSION     DAXWOO010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.340"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(<1..>903)
                     /locus_tag="ACGE70_002977"
     rRNA            complement(<1..>903)
                     /locus_tag="ACGE70_002977"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          193 a          277 c          203 g          230 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 ggg
//
LOCUS       DAXWOO010000331          898 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.341, whole genome shotgun sequence.
ACCESSION   DAXWOO010000331 DAXWOO010000000
VERSION     DAXWOO010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..898
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.341"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(127..675)
                     /gene="aac(6')"
                     /locus_tag="ACGE70_002978"
     CDS             complement(127..675)
                     /gene="aac(6')"
                     /locus_tag="ACGE70_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFF8694522.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          258 a          188 c          137 g          315 t
ORIGIN      
        1 gtacacggtg ttccaaaagt agcttcttcg gaaataagtt ggaatttcac aaaaattcaa
       61 gaagtaattt tcgtaaattc cttcttattt cttgaagctg attacttctg tcacaacctc
      121 tttttattat tgagaatttg gtcgaggaat aatcgttttt gccatccaaa tatccggttt
      181 gtcccagcca tttgcatttg gtaagacacc tacgatttta taacctaatt tttcatagaa
      241 ttcatacgga tgttcacgaa ggttctggat ggaagccact ttatcaaatg tatgctcata
      301 caggtccgtt tgacttaacg ttgttccatg gtctaaatca tccgtaccta aataaatcgt
      361 gattcctcct ctggaagcta cttctttttc taagtaattg actaatcgag tacctatttg
      421 gttctttcgt cgggagcttt ctacaactaa tggatgcaat tcccaacctg tgataccgta
      481 ttgagggatt gcaccaataa atcctactaa ctcatcttgg tctaccgctg ctaccgcgat
      541 tcgttctgga ttcatcattt cttctacttc ttctgccgag ctgtctccat attcttccgg
      601 ccaagtcagt ctcagtaaat cagaaagctg atctttcaat actggattat tacggtcaaa
      661 ttcactgatt atcatacgtc ttcccccttg ttcttgctta ttctaacagt ccttttgaaa
      721 aaacgaaatg ttctcgctca aaaatcttta gctaccaatc agtatctcta ttctttcatt
      781 atttatctta cttataaaat aaaaaaatta tacagaaaca ctttattcaa aataggaaag
      841 atcaggaaag ccatcaattg gaggaaaaaa ctctgtttgt tcatcatgat atagatta
//
LOCUS       DAXWOO010000332          893 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.342, whole genome shotgun sequence.
ACCESSION   DAXWOO010000332 DAXWOO010000000
VERSION     DAXWOO010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..893
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.342"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..>893
                     /locus_tag="ACGE70_002979"
     CDS             <1..>893
                     /locus_tag="ACGE70_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFF8694523.1"
                     /translation="FVSTKDDLWIYVFITSITNFLGSLVFWLSIHGKVGKPLFNSNVF
                     KQLWKPAFIILTPQLFMQVYTTLDKTIVGYFVDPTALSYYDQSQKIARIILAVLTSIT
                     IVMLPKISKAQAEGKSENILVYTKKSFDYTIIIALILFSVVFINTKEFVPWFFGENFK
                     PMTINMLISCLIIVVSPIGGVFANQFAIAIEKDKEYAYPMIIGAIVSIIGNVAFVPTY
                     GAVAATIVLVVVELLVFFFRVFFVRKEIKISYFFGKNVVLFFIVNLTLTTSAYYFLPQ
                     LFSSSFLDMAIKSIMIFIIYLP"
BASE COUNT          266 a          127 c          146 g          354 t
ORIGIN      
        1 tttgtttcaa ctaaagatga tttgtggatt tatgtgttca ttacttcaat tactaacttt
       61 ttaggaagtt tagttttctg gttgagtatc cacggaaaag taggtaaacc actctttaac
      121 tctaatgttt ttaaacagtt gtggaaacca gcttttatta ttttgactcc tcagttgttt
      181 atgcaagtgt acacaacttt agacaaaacg atagttgggt attttgtgga tccaacagca
      241 ttatcttatt atgatcagtc tcaaaaaatt gctcgtatta tattggcagt cttaacatca
      301 attactattg ttatgttgcc taaaatatct aaagcacagg cagagggaaa atcagaaaat
      361 attttagttt acacaaaaaa atcttttgat tacacgatta ttattgcatt aattcttttt
      421 agtgtggtgt ttatcaatac aaaagaattt gttccatggt tttttgggga gaatttcaaa
      481 ccgatgacaa taaacatgct tatttcttgt ctaattatag ttgttagtcc aattggtgga
      541 gtttttgcta accaatttgc aattgctata gaaaaagata aggagtatgc ttatccaatg
      601 atcattggtg cgattgtaag tattattgga aatgtagctt ttgtgcctac ttatggcgct
      661 gtagcggcta caatcgtact cgtggtagta gaattattag tctttttctt tagagtattt
      721 tttgtacgaa aagaaataaa aatatcctat ttctttggga agaacgttgt cttatttttt
      781 atagtcaatc ttactttgac tacttcggcc tattactttc ttccacaatt attctcttct
      841 tcatttttag atatggcaat taaatcaata atgattttca ttatttatct tcc
//
LOCUS       DAXWOO010000333          890 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.343, whole genome shotgun sequence.
ACCESSION   DAXWOO010000333 DAXWOO010000000
VERSION     DAXWOO010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.343"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..888
                     /locus_tag="ACGE70_002980"
     CDS             <1..888
                     /locus_tag="ACGE70_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFF8694524.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT          386 a          116 c          144 g          244 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt
//
LOCUS       DAXWOO010000334          883 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.344, whole genome shotgun sequence.
ACCESSION   DAXWOO010000334 DAXWOO010000000
VERSION     DAXWOO010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..883
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.344"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            61..753
                     /locus_tag="ACGE70_002981"
     CDS             61..753
                     /locus_tag="ACGE70_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFF8694525.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          383 a          139 c          152 g          209 t
ORIGIN      
        1 attgtgtttg agatcactta cttttagcat gtaaaagtat tggattaagg agctatgcaa
       61 atgaaaaaga aatttattac tggtgggatt gtcgttgcat ttctatgtct aattggctac
      121 acaggcacgc ggtacgcaaa tgaaattatt ttgtggggtg gagagacaga tattgataca
      181 atcaatgaga atctgacaca gcttgatcat acattgacag ataaagagca agcgattaca
      241 gatcttaccg ctcgactagc acaaaaagaa cagacattgc aagattcaaa ggcagatgtc
      301 caagaatacc aaacaaaagt aaatgaatta gaaaaccaaa aaaatcaatc ggcagcagaa
      361 aaacaaaact tagaaagtca attagtcagc aaggatgcac aactacaaag caaacaaaat
      421 gaaatcaatg tcaaactaga tgagatcaac caaaaaaatg atgaaatcaa tcatctgaat
      481 gcagattgga cagcaagact acaagaagca caagcaaaac tttcagaatt acaaaatact
      541 gtaaatggtt tgaatcaaca aatcgcaaca ctgacacaag aaaaaacaaa tgtagaaact
      601 cagttaacag atacacaaaa gaaattagac gaagcaactg ctgaaaactc aagcttgaaa
      661 caatatattc aaaagttaga aaaagcaaaa gatgaagtgg aagatacagc tgataaatct
      721 cagcaaattg ttgaagaaca tttgcgtaaa taatctttct taacaagtaa acctaaatta
      781 gtaaagaagc aacagtaatc tatactaagt agttcagttt tggagtacaa aatgacaaaa
      841 tgtacttcaa aatggactac ttttttcatt ttaacttaca tac
//
LOCUS       DAXWOO010000335          866 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.346, whole genome shotgun sequence.
ACCESSION   DAXWOO010000335 DAXWOO010000000
VERSION     DAXWOO010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.346"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(53..125)
                     /locus_tag="ACGE70_002982"
     tRNA            complement(53..125)
                     /locus_tag="ACGE70_002982"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(90..92),aa:His,seq:gtg)
     gene            complement(128..201)
                     /locus_tag="ACGE70_002983"
     tRNA            complement(128..201)
                     /locus_tag="ACGE70_002983"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(167..169),aa:Trp,seq:cca)
     gene            complement(208..288)
                     /locus_tag="ACGE70_002984"
     tRNA            complement(208..288)
                     /locus_tag="ACGE70_002984"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(252..254),aa:Tyr,seq:gta)
     gene            complement(290..365)
                     /locus_tag="ACGE70_002985"
     tRNA            complement(290..365)
                     /locus_tag="ACGE70_002985"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(330..332),aa:Phe,seq:gaa)
     gene            complement(401..473)
                     /locus_tag="ACGE70_002986"
     tRNA            complement(401..473)
                     /locus_tag="ACGE70_002986"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(438..440),aa:Asp,seq:gtc)
     gene            complement(483..555)
                     /locus_tag="ACGE70_002987"
     tRNA            complement(483..555)
                     /locus_tag="ACGE70_002987"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(520..522),aa:Val,seq:tac)
     gene            complement(571..642)
                     /locus_tag="ACGE70_002988"
     tRNA            complement(571..642)
                     /locus_tag="ACGE70_002988"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(607..609),aa:Glu,seq:ttc)
     gene            complement(657..746)
                     /locus_tag="ACGE70_002989"
     tRNA            complement(657..746)
                     /locus_tag="ACGE70_002989"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(710..712),aa:Ser,seq:gga)
     gene            complement(786..859)
                     /locus_tag="ACGE70_002990"
     tRNA            complement(786..859)
                     /locus_tag="ACGE70_002990"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(823..825),aa:Asn,seq:gtt)
BASE COUNT          241 a          238 c          198 g          189 t
ORIGIN      
        1 gggagtcaaa gtcccttgcc ttaccgcttg gctatacccc aagaataaaa agggcgaatg
       61 atgggaatcg aacccacgaa tgccggagcc acaatccggt gcgttaacca cttcgccaca
      121 ttcgccatgg caggggcagc aggaattgaa cccacactaa cggttttgga gaccgctgtt
      181 ctacctttaa actatgcccc tataaaatgg aggagagtgg attcgaacca ctgaacccta
      241 aggaacggat ttacagtccg tcgcgtttag ccacttcgct actcctccat ggtggcgcgg
      301 gacagaatcg aactgccgac acacggagct tcaatccgtt gctctaccaa ctgagctacc
      361 gcgccatatt ttaaaattat taattgaata acaagaacga cggttccgac gggaatcgaa
      421 cccgcgatct cctgcgtgac aggcaggcat gttaacccct acaccacgga accagttcat
      481 tatggaggtt aacgggatcg aaccgctgac cctctgcttg taaggcagat gctctcccag
      541 ctgagctaaa cctccaacat tcaattaaaa tgacccgtac gggattcgaa cccgtgttac
      601 cgccgtgaaa gggcggtgtc ttaaccgctt gaccaacggg ccaggtaaat ttgctacgga
      661 gagtaaggga ttcgaaccct tgagacagtt tggcaccatc tacatgattt ccaatcatgc
      721 tccttcggcc tctcggacaa ctctccagag tgaaagaagc tcaatactct ttctcaaaat
      781 gagaactccg gcagtaggac tcgaacctac gacatcatga ttaacagtca tgcgctacta
      841 ccaactgagc tatgccggaa taacat
//
LOCUS       DAXWOO010000336          830 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.347, whole genome shotgun sequence.
ACCESSION   DAXWOO010000336 DAXWOO010000000
VERSION     DAXWOO010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.347"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..830
                     /locus_tag="ACGE70_002991"
     rRNA            <1..830
                     /locus_tag="ACGE70_002991"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          204 a          178 c          262 g          186 t
ORIGIN      
        1 catggagctt tactgtagtt tgatattgag tgtctgtacc gcatgtacag gataggtagg
       61 agccgtagaa atcggaacgc tagtttcgat ggaggcgctg gtgggatact acccctgcgt
      121 tatggccact ctaacccgca ccactaatcg tggtgggaga cagtgtcaga tgggcagttt
      181 gactggggcg gtcgcctcct aaaaggtaac ggaggcgccc aaaggttccc tcagaatggt
      241 tggaaatcat tcgaagagtg taaaggcaga agggagcttg actgcgagac caacaagtcg
      301 agcagggacg aaagtcgggc ttagtgatcc ggtggttccg catggaaggg ccatcgctca
      361 acggataaaa gctaccctgg ggataacagg cttatctccc ccaagagtcc acatcgacgg
      421 ggaggtttgg cacctcgatg tcggctcgtc gcatcctggg gctgtagtcg gtcccaaggg
      481 ttgggctgtt cgcccattaa agcggcacgc gagctgggtt cagaacgtcg tgagacagtt
      541 cggtccctat ccgtcgcggg cgttggaaat ttgagaggag ctgtccttag tacgagagga
      601 ccgggatgga cttaccgctg gtgtaccagt tgttctgcca agggcattgc tgggtagcta
      661 tgtagggaag ggataaacgc tgaaagcatc taagtgtgaa gcccacctca agatgagatt
      721 tcccatttct ttaagaaagt aagatccctg agagatgatc aggtagatag gtcaggagtg
      781 gaagtacagt gatgtatgga gcggactgat actaatcgat cgaggactta
//
LOCUS       DAXWOO010000337          811 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.348, whole genome shotgun sequence.
ACCESSION   DAXWOO010000337 DAXWOO010000000
VERSION     DAXWOO010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.348"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..672
                     /locus_tag="ACGE70_002992"
     CDS             <1..672
                     /locus_tag="ACGE70_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFF8694526.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          298 a          126 c          152 g          235 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcaggttt a
//
LOCUS       DAXWOO010000338          802 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.349, whole genome shotgun sequence.
ACCESSION   DAXWOO010000338 DAXWOO010000000
VERSION     DAXWOO010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.349"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(301..387)
                     /locus_tag="ACGE70_002993"
                     /pseudo
     CDS             complement(301..387)
                     /locus_tag="ACGE70_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          292 a          135 c          106 g          269 t
ORIGIN      
        1 tgtctgctac cccggcctaa tctggcaagg ggaggaggag atataatgaa ataccttttt
       61 gattttgttt ttgcaatcaa gccacttgca gatgtagtgt gcagttacct ctgcaaatgg
      121 cttgattgca aaattgccaa aaaagacagt aagcctaccc gttaattcta taatatagaa
      181 acgtaaaaaa aaccccatca gcctgcacgc tgatggggtt tggtgatata atgaaatacc
      241 tctttgttac tcataattat accataatta gtcacgaatg caaccaatag aaaaaactac
      301 ttagaacggt agatcatcgc taataacaat gcattgtcca ccaaatggat tttgtgaagc
      361 attaaataca ttgttattaa ttgtcgctgc ttttaactag gcctctcccc ttctattata
      421 aaagaagata tatatcattt cttaattacc tactacctta aaccaaaatt taagtactac
      481 ctaatttaag tagtacttaa acaatctaaa aaatagttat cccaattgtt atgcttttat
      541 aatacgatta tttgtaacaa aatgaatatt atataattaa acactaccat atttaggtag
      601 tgtttaatta ttagcaaaac aaaaaatatt tattctattc tcttaacttc aactaagtta
      661 tgctgcacac ctttatataa atcatatgca actcattagc tagacaaact acaagcatgt
      721 agttttatct gttaattatg tactacctaa ataaagtagt ttataattag caagtatata
      781 tatttaaaaa tttaagatta at
//
LOCUS       DAXWOO010000339          781 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.350, whole genome shotgun sequence.
ACCESSION   DAXWOO010000339 DAXWOO010000000
VERSION     DAXWOO010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.350"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            9..101
                     /locus_tag="ACGE70_002994"
     CDS             9..101
                     /locus_tag="ACGE70_002994"
                     /inference="COORDINATES: protein motif:HMM:NF021542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694527.1"
                     /translation="MKTVILTVLTICGFYLGNYLEIVGFFDKQN"
     gene            157..735
                     /locus_tag="ACGE70_002995"
     CDS             157..735
                     /locus_tag="ACGE70_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFF8694528.1"
                     /translation="MENKTNNTKTEIKKNDVAKTSEIIKGELVFEDKVIQKIIGIALD
                     EIDGLLTIDGGFFSNIAGKLVNTDNTTSGVDVEVGKKQVAVDLSIVAEYGKDVTTIYD
                     KIKEVISKEIKKMTGLDVIEVNVDVVDVKTKEQHENDSVTLQDHLSDAASTTGEFASK
                     QFEKSKEALGVASEKVSDGVENVKEASEPRVE"
BASE COUNT          317 a           87 c          149 g          228 t
ORIGIN      
        1 taggatttat taaaacagta atattaacag tgctgactat ttgtggtttt tatttaggta
       61 actatttaga aattgttggt ttttttgaca agcaaaatta gttaataacc gtttttacgg
      121 taagaaataa atttctaatt tttaggagga aaaataatgg agaacaaaac aaataataca
      181 aaaacagaaa tcaaaaagaa tgacgtggca aaaacttccg agataattaa aggagaacta
      241 gtttttgaag ataaagtaat tcaaaaaata attggtattg cattagatga gattgatgga
      301 cttttaacga ttgatggggg cttcttctca aatatagctg gaaaattagt aaatacagat
      361 aacacaactt ctggagttga tgttgaagta ggaaaaaaac aagtcgcagt agatctttca
      421 atagtggctg aatatggcaa agatgtaact acaatttatg ataaaataaa ggaagttatc
      481 tcaaaagaaa ttaagaaaat gactggctta gatgtaattg aggttaatgt agacgtcgta
      541 gatgtaaaaa cgaaagagca acatgaaaac gattcagtta cactacaaga tcatctttcc
      601 gatgcagctt ctactactgg agaatttgct tcaaaacaat ttgaaaaatc gaaagaagct
      661 ttaggagttg caagtgaaaa ggtaagtgac ggtgtagaga acgtaaaaga agcatctgaa
      721 cctcgcgtag aataacaaat cttttttatt taatgtacaa tctataaaat taaaagactg
      781 t
//
LOCUS       DAXWOO010000340          768 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.351, whole genome shotgun sequence.
ACCESSION   DAXWOO010000340 DAXWOO010000000
VERSION     DAXWOO010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..768
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.351"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(109..>768)
                     /locus_tag="ACGE70_002996"
     CDS             complement(109..>768)
                     /locus_tag="ACGE70_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HFF8694529.1"
                     /translation="RIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQ
                     PLEAIVTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLT
                     DVKLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQY
                     QFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEA
                     A"
BASE COUNT          213 a          176 c          147 g          232 t
ORIGIN      
        1 gacaaaaatt ataaggtgtt gtgaatgctg cggtgttatg gctacgattg cccttgacaa
       61 tgagcatcct cggatctgat tttctccggc agaagcacaa tcattcaatt acgctgcctc
      121 tccagaggta tcagatccgc gctcattatc aaggattcgc ttcgccaatc tctgttgtcg
      181 aatttggatc ggtgtttcat acgccaacgt gccatgtaag cgtagatagt tccaccaatg
      241 cacataatca aacaattcta gacgtagctg tgccagtgtc tcaaattggt attgatgcac
      301 gaattctact ttgacagatt tataggttga ttccacaacg gcattatcat acggacaacc
      361 cttcctactc aatgaacgag taattccaaa accattcaga atctcatgaa tggtttggtt
      421 atcaaattcc tttccccggt ctgtatgaaa aagcttgacg tctgttaaag aatacggtat
      481 ccgtccaaag gcttctttta ccaatgaggc atctttcttc tcaccacagg aataaccaat
      541 aatttctcga ttaaacaaat caagtattaa gcagatataa tgccactttt tccccacgcg
      601 aacataagta agatccgtaa cgatggcttc caatggctgt tcttgtgtaa aggtccgatc
      661 taatacattc gctgttttcg cttcattaca agctgttcgt tgccctttaa aatgagcgat
      721 cgtataggta gatgtcaatc cgcggcgttt catgattcga ccgattcg
//
LOCUS       DAXWOO010000341          763 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.352, whole genome shotgun sequence.
ACCESSION   DAXWOO010000341 DAXWOO010000000
VERSION     DAXWOO010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.352"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            193..600
                     /locus_tag="ACGE70_002997"
     CDS             193..600
                     /locus_tag="ACGE70_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694530.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
BASE COUNT          279 a          105 c          134 g          245 t
ORIGIN      
        1 gtttgttcct tatatagagg cacttttact actgattaaa tgtcagatat gcttacaaat
       61 atacttataa atatgcttac aaaattagct aatatttccg ttgacaaagc tttgttacta
      121 ttgttaaact aaattgacta gcaaaagtga aacataaatt tattaaataa atgtaaaaat
      181 taggagaaat caatgtctta tagagtacaa ttaataattt cagaagatgt agaaagtcag
      241 caatttggca ctaatgttat taataaagta attaatccag ctctttctat taatgcgcct
      301 ttaattccta ctgctttatc attttctgta acagctgtgg tgtctgagat agaggataca
      361 gaaaagataa aaatagtaga aattgaagtt ttaaacaaga atgaaaaaca aattttttct
      421 acgggtgaag tatcggtaag cttgcctcct caagttaacg atataaactt taatataaac
      481 gccagaaatg tcttggttga agaagcagga gagcattatg ctgttgttaa atttaatgga
      541 actgagattg gaaggcaaat ctttgatatc aaggtcaaca aaccagtgga aaaaaattaa
      601 ggagacgata cggatgcttg atatagtacc atcaaataca catcggagtg gtagcttaat
      661 aaattttgct gcgatttttg cttgcgcatc ggtaattcta gcatctccag ttgttgtgga
      721 tgcaagttct actccgaaaa ctaaaaatga taatcaactt gta
//
LOCUS       DAXWOO010000342          741 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.353, whole genome shotgun sequence.
ACCESSION   DAXWOO010000342 DAXWOO010000000
VERSION     DAXWOO010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.353"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(<1..298)
                     /locus_tag="ACGE70_002998"
     CDS             complement(<1..298)
                     /locus_tag="ACGE70_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFF8694531.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLV"
     gene            complement(428..>741)
                     /locus_tag="ACGE70_002999"
     CDS             complement(428..>741)
                     /locus_tag="ACGE70_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFF8694532.1"
                     /translation="IFENKRIFDLILFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKT
                     IGMINQNLVFNEEEIHFYIYSFYATFYDILKHSYSKEKTMLLTRRLYQFSVPGWIELL
                     S"
BASE COUNT          289 a          124 c           93 g          235 t
ORIGIN      
        1 taaccaacaa ccatgagatc attacgaccg gcaacattcg aaatacaata tataaggatg
       61 tcgcaaaaaa tatagtaaaa aactctatct tgatagcatc aataattttc attactaaaa
      121 acagaactga ataaatcaag atgaaataaa tacatgtctt tattttacga aacatcaata
      181 ctaaaactgt agcaataatg aaacacccca cttgaaatat atttcccttt cctaaaaaaa
      241 taatgaccgg aaaaaaaata tttaaagtaa tcaaggttaa cggattgatt tttgacactc
      301 tttcacctcc aaaaaataag atcgcactgt tatcttaagc tgataaaaat gcaccgttat
      361 tcagataaca gtgcgatctt atcaaaaagc ataactgaga acaattctca atgcaagctt
      421 tttttaatta actcagtaat tcaatccacc caggaacaga aaattgatac aatcttctgg
      481 ttaatagcat agtcttttct tttgaatagc tatgcttcaa tatatcatag aatgtagcgt
      541 agaatgagta aatgtagaag tggatttctt cttcattaaa gactaaattt tgattgatca
      601 ttccgatagt ctttaactta tctagacaac agactgtctt tttcgtcaca aatgatgtaa
      661 ttttttcaac aatatcttga tatttggttc cagctgattg gaataaaatc aaatcgaata
      721 tacgtttatt ctcaaaaata t
//
LOCUS       DAXWOO010000343          739 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.354, whole genome shotgun sequence.
ACCESSION   DAXWOO010000343 DAXWOO010000000
VERSION     DAXWOO010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.354"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            332..>739
                     /locus_tag="ACGE70_003000"
     CDS             332..>739
                     /locus_tag="ACGE70_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFF8694533.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNL"
BASE COUNT          244 a          140 c          118 g          237 t
ORIGIN      
        1 agttataatc atagttgtta ggtgattcat ctagtaaaaa caccagattt caaatttcac
       61 acaatttaaa ctagatcaat catcaacctt cttcaaattg ttagaaccct ttcccgagga
      121 gctgcagcat aagcaaaaaa acaaacagga agccaaatgg ctaagacatt ttttctatta
      181 gtctagttgt ttctcgtcgc aagtttaaga ctttagaaag ttatttctaa tgatgtttgg
      241 gttgttttga tcaatgctta tgcacagctc cttacctatt atttatctca acagatctaa
      301 caaattaatg gactatttaa ggagtttttt tatgcttacg atctattctt ctcgcacaca
      361 tagttaccat ctgatggcaa actggctgaa agaccatcaa atgccttaca gagaacgaat
      421 tttcacacct aaacagcctt taacaaggaa agagatattt tatttattgt cattaacaga
      481 aaacggattt gacgatctat tagctaaacg ctcgattgat tataaaaatt tgaaagacca
      541 aatcgaccaa tgtacgacct cagagcttgt cgatctgatc gtctcgcatt ttactttgtt
      601 acaacgtccc atcattactg acggaaagaa attattagtc ggattcaatg aagatagcat
      661 gcgtcttttt atccctgtac aatggcgcag aagtcaactg aaatctgtct ataacgattt
      721 tacatgggta agcaatcta
//
LOCUS       DAXWOO010000344          695 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.355, whole genome shotgun sequence.
ACCESSION   DAXWOO010000344 DAXWOO010000000
VERSION     DAXWOO010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.355"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(53..628)
                     /locus_tag="ACGE70_003001"
     CDS             complement(53..628)
                     /locus_tag="ACGE70_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HFF8694534.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
BASE COUNT          229 a          151 c          124 g          191 t
ORIGIN      
        1 atgaaaaaac agagccagat tagcattatt ttgctaatcc agctctgttt attcagccta
       61 tttttctttt tgaatatatc aagcgttggg gcaacatagt gatgactaat gaaacaacga
      121 cgatagacaa caagcgatca aggtagtctg tacccgcctg taccaagatc acacttgttg
      181 tctgacttaa acctaaccca tgtaatagct gaacgatcat actggatcct gaggaggtga
      241 tcccgccaaa taactgaacg gtaatgatcg aagaaacgat cgttccaggt accgacaaaa
      301 tcaaagcgag taataacagt ttcaagctgt ttttatcttt gccgagatat cttggcaaaa
      361 tcaatccagc aagcaagcct gtgagaattt ggattggcga ataatacaga gaaaaaatat
      421 ctgtagtcac tccacttaaa ataccgctgg acaatcctgt cagcattcca ggtacaggcc
      481 caagcaatgc accagcaaaa atcgttccga tagaatcgag atagattggc aaacgaagcc
      541 ataaagcaat cgttcctcca atataattga tcccaataca caaagccatc actgtaagta
      601 cgcgagttgc cattttgttt ttattcattt tgttagccca tctttaacct ttccaaatct
      661 ttcataatcg tttcttcttg tgcatttaaa agaac
//
LOCUS       DAXWOO010000345          684 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.356, whole genome shotgun sequence.
ACCESSION   DAXWOO010000345 DAXWOO010000000
VERSION     DAXWOO010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.356"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..268
                     /locus_tag="ACGE70_003002"
     CDS             <1..268
                     /locus_tag="ACGE70_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
                     /protein_id="HFF8694535.1"
                     /translation="VINKNTSLHEESLIIIEALGGKENIESVEACATRLRVAVTNGEI
                     IQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            281..>684
                     /locus_tag="ACGE70_003003"
     CDS             281..>684
                     /locus_tag="ACGE70_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFF8694536.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEK"
BASE COUNT          238 a           95 c          132 g          219 t
ORIGIN      
        1 agtaataaac aaaaatacat cattacatga agagtctctt atcattattg aagctcttgg
       61 tggaaaagaa aatattgagt ctgttgaagc ttgtgcaact cgtttgcgtg ttgctgttac
      121 gaatggggag attattcaaa aacaaacaat aaaagacact ggtgcaactg ctgtcttcga
      181 agtgaagggt ggcatccaag ctgtttttgg agggaaagct gacttattaa gtcaagaaat
      241 caatcaaata ttaggaaaag ataattagga ggtttttaat atgtttgaaa ttttcaaaaa
      301 gaaaaaacaa gaaacttttt atgcaccttg ttccggaaaa atcatttcca tcacagaagt
      361 aaatgatcct gtcttttcac aaaaattact aggtgatggt tttgccgtca tacctgatgg
      421 ttcaaaaatt tatgctccat tggctggtcg agtaacaaat atatttccta ctaaacatgc
      481 tattaatata aaaagcaact caggcattga ttatttgatt catattggaa ttgatacagt
      541 tgaattacaa ggagagcctt tttcgatttg tgttcaagaa aatgatgagg ttactcctga
      601 aacctcgttg gttgatgtgg attttgacaa aataataaaa tctggtaaag atacttctgt
      661 tatagttgtt tttccggaga aaaa
//
LOCUS       DAXWOO010000346          679 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.357, whole genome shotgun sequence.
ACCESSION   DAXWOO010000346 DAXWOO010000000
VERSION     DAXWOO010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.357"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..340
                     /locus_tag="ACGE70_003004"
     CDS             <1..340
                     /locus_tag="ACGE70_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFF8694537.1"
                     /translation="NIGSFGEDIYKIGMTRRLEPMDRIKELSSASVPFEFDVHAMIFS
                     EDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLDEIEKVVLENYNGTVTFTKLAKAEQ
                     YRRSLELSKD"
     gene            412..>679
                     /locus_tag="ACGE70_003005"
     CDS             412..>679
                     /locus_tag="ACGE70_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694538.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQ"
BASE COUNT          287 a           86 c          115 g          191 t
ORIGIN      
        1 taatattggg tcttttggtg aagacatata taaaattggt atgactagaa gattagaacc
       61 tatggataga ataaaagaat tgagtagtgc atcggttcca tttgaatttg acgttcatgc
      121 gatgattttt tcagaggacg ctccgtcttt ggaaactaaa ttgcataatc attttagaaa
      181 acaagaagtt aataaaatta atcaaagaaa agaatttttc aaagtttcac tagacgaaat
      241 agagaaagtt gttttagaaa attataatgg aacagtaaca tttacaaaat tagctaaagc
      301 tgaacaatat agacgatctt tagaattgtc aaaagattaa atatataaaa agccttcggg
      361 ctttttcttt taacaattaa taggaacgta tattcgaaag gaatagaaat aatgtggtta
      421 gaaaaaataa atgacaaaaa atttaagtac accgaacgtt atacggatcc cttaactgaa
      481 aaaaagagaa aagtgtccgt tactttatct agcaattcac gccaagcttg gaatcaagct
      541 aacttacttt tgaatgaaaa aatcgcagaa aaaataaaac gaaatgaaga attaccacta
      601 acttttggag aattaaaaac taaatgggat gaaaaataca agccaactgt aaaagaaagt
      661 tcctatcgaa caactcagg
//
LOCUS       DAXWOO010000347          674 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.358, whole genome shotgun sequence.
ACCESSION   DAXWOO010000347 DAXWOO010000000
VERSION     DAXWOO010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..674
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.358"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            89..283
                     /locus_tag="ACGE70_003006"
     CDS             89..283
                     /locus_tag="ACGE70_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694539.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
BASE COUNT          281 a           85 c          112 g          196 t
ORIGIN      
        1 tatttatcat aaagatatag atacataatc attttcactt acatatattt gaatattaac
       61 taaaaacagc aagaaagaag aggatcaaat gtattatgta gaagtaaaaa caaaaggcgt
      121 caaaaacaaa caatatgtga aaggtatcag taacgaatat cctttacttg gttcatggaa
      181 agaagctgct cctttttcca aaccatgtgc cataaaaata aagaacgagc tagaaaaaga
      241 actgacttgt ggtaaagctg ttgtagatat catcgaaaag taaatccaat gtatagaaag
      301 gaagatcaca catgaaaagc gacgtacaaa ttgctaagat tatagaatta gaagaagaag
      361 acgcagtgtt gacaattgaa aaaaaataaa tgtttgtata ccatatgatt atttcaatat
      421 gtatcctgga attggagatc tcgtagagct atatcaagat gaatcaaata tacttgtcat
      481 tcatcatgaa atatagagac ttataaaatt gtttattttt ataatgtatc agtatagaaa
      541 ggaaataaaa catggattat actgttgaat ttgacagtga actacttaag caatttccac
      601 aagaagaaca tttcatgtat aaagaagcaa tttcccacaa agtgacagag tatttggagc
      661 ataatttttt tcgt
//
LOCUS       DAXWOO010000348          656 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.359, whole genome shotgun sequence.
ACCESSION   DAXWOO010000348 DAXWOO010000000
VERSION     DAXWOO010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.359"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(281..478)
                     /locus_tag="ACGE70_003007"
     CDS             complement(281..478)
                     /locus_tag="ACGE70_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFF8694540.1"
                     /translation="MNNGTVKWFNSDKGFGFITGEDGNDVFAHFSAIQGEGFKSLDEG
                     QAVTYDIEEGQRGPQAVNIVK"
BASE COUNT          229 a          118 c           90 g          219 t
ORIGIN      
        1 attgtgatcc acctcttttg tttattagtt taaaccatca aaccattttt aataaaatat
       61 gtaaattcct ttgctcaaaa aagtagcttc tttacgattc gacaatttca aataaagcag
      121 gtaaaaactg gctgtcagaa aatagccaaa ctgaagcaaa aaataatgtt aaaattaatt
      181 aatgaacgaa aaaaatccct cacctaatat tacggtgagg aattttttct accgacataa
      241 acaaaaaaac cacctcatat gaggcggtta acgttcaaca ttattttacg atatttactg
      301 cttgaggacc acgttgtcct tcttcaatat cataagtaac tgcttggcct tcatctaaag
      361 acttaaagcc ttctccttgg attgctgaga aatgagcgaa tacgtcattt ccatcttcac
      421 cagtgatgaa accaaaacct ttatctgagt taaaccattt tactgtaccg ttattcatat
      481 atatttcctc ctgatacgta tataatacgt gttttattgc aattaatatt acttggtaaa
      541 aggagttttt atagtgtgaa tatatcgctc agattacgtt tcaaattaga ttacttattt
      601 actataacac ggatgtaatg ctaacacaag gataatcact cgatatacgg tttaaa
//
LOCUS       DAXWOO010000349          645 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.360, whole genome shotgun sequence.
ACCESSION   DAXWOO010000349 DAXWOO010000000
VERSION     DAXWOO010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.360"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(<1..208)
                     /locus_tag="ACGE70_003008"
     CDS             complement(<1..208)
                     /locus_tag="ACGE70_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694541.1"
                     /translation="MDKGVADIAKIKQVLKQESIKSLVEGTGLSKSTISSLKSGTRKV
                     EKLNLFAAIKLTEYSDQVFKPIIEI"
     gene            complement(463..>645)
                     /locus_tag="ACGE70_003009"
     CDS             complement(463..>645)
                     /locus_tag="ACGE70_003009"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694542.1"
                     /translation="RFNFNDKVIFTQNLKTNNENEKLYEPTNIDTQDEKTNNTTKVYD
                     GWYYLGSLSNRTDRVI"
BASE COUNT          227 a           99 c           74 g          245 t
ORIGIN      
        1 agatttcaat aataggttta aaaacttgat ctgaatattc ggttagttta atagctgcaa
       61 aaaggtttaa tttttctact tttcgagtac ctgattttaa acttgaaata gtactcttac
      121 ttaaaccagt tccttctact aagcttttta tagattcttg tttcaacact tgcttaattt
      181 ttgcaatatc agcgactcct ttatccaaaa aaagactcct tttttatctc aaatttataa
      241 ctactgtagt aaaaatatag catattttag tatgctctgg tagtaaaagt ttaaaagtac
      301 gaaaagggaa caaatataca ataaaaataa attgtattac ataagcaaca tagatatact
      361 ccaacatcct atttatctga ataaaggaac ctttcaaaac aaaaaattat tttgatggac
      421 gtctaatcca gcataatact cataattata gagccgatgt tcttatatca ctctatcagt
      481 cctatttgac aaagagccta aataatacca accgtcataa acttttgttg tattattagt
      541 tttttcatct tgggtatcaa tatttgttgg ttcataaagc ttttcatttt cattatttgt
      601 ttttaaattt tgagtaaata tcactttatc attaaaatta aaccg
//
LOCUS       DAXWOO010000350          645 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.361, whole genome shotgun sequence.
ACCESSION   DAXWOO010000350 DAXWOO010000000
VERSION     DAXWOO010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.361"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(138..491)
                     /gene="tnpB"
                     /locus_tag="ACGE70_003010"
     CDS             complement(138..491)
                     /gene="tnpB"
                     /locus_tag="ACGE70_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFF8694543.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
BASE COUNT          192 a          141 c          108 g          204 t
ORIGIN      
        1 ttgaagcaac tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa
       61 ctgattttgt tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt
      121 tgtgttaacc ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga
      181 tcgatactta atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg
      241 tctttcgtcc gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa
      301 ccatcaccgt cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa
      361 acggcatctt cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca
      421 attccttttc gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa
      481 tcataaagca ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc
      541 gttatagatc aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc
      601 cgcgggttta cctttttctg gtgcactctt ttcgtttcct aattg
//
LOCUS       DAXWOO010000351          644 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.362, whole genome shotgun sequence.
ACCESSION   DAXWOO010000351 DAXWOO010000000
VERSION     DAXWOO010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.362"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            14..613
                     /locus_tag="ACGE70_003011"
     CDS             14..613
                     /locus_tag="ACGE70_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFF8694544.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT          244 a           95 c          147 g          158 t
ORIGIN      
        1 ggaggctcta caaatgaacg gttttgaaaa gagagcacaa gagaaaaaga aagagatttt
       61 acaagcagct tttgacttga tgaacacaga cataggaacc gctggagtga caatggataa
      121 ggtagctgag aacgctcagg taagtaaagc gacgattttt aaatattttg gcagcaagga
      181 agggctgatt tatgaagtat tttttgcatt tatgaatgat ttagggcaag aagcgaaagc
      241 gttgattgcg gaaaatcttc catttgaaga aacactgatt gccatgagtg aaaacaaaat
      301 ccgttatctc aatcgagtga acaaacaatt ttatatcgat ttgatgacat actatacaaa
      361 aaaagaaaaa aaagaatttt ctgacgcgat ggatgcgtat acgcaagaaa gtttcagtat
      421 catgcttgat cttttccatc gagggcggaa agaaggaaaa gtggatttga aatattcgga
      481 tgagttcttg atcctctatt ttcaagcaat ggtagaagga atttctaagc cggagatata
      541 cgcaaaactg cttccatata cagcagaatg gacagaagtc ctgatcaagg gaatcgcacc
      601 gaagaagaaa taaagcataa aaaagccgaa actgacgata aaat
//
LOCUS       DAXWOO010000352          625 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.363, whole genome shotgun sequence.
ACCESSION   DAXWOO010000352 DAXWOO010000000
VERSION     DAXWOO010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.363"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..>625
                     /locus_tag="ACGE70_003012"
     CDS             <1..>625
                     /locus_tag="ACGE70_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFF8694545.1"
                     /translation="MTKSFTEEVTSFKERELHDRYAAIYMDATYIPLKRKTVAKEAIH
                     IAVGIRPDGSKEVLSYAIAPTESITIWEEILIDLQERGVKNVLLFITDGLKGMAGAVQ
                     RFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMVYQASSKKAALEARGAFAEKW
                     KTSYPKMVESILSNDYLLTFYDFPLAIRKTIYSTNLIESFNKQIKKYS"
BASE COUNT          183 a          134 c          140 g          168 t
ORIGIN      
        1 atgactaaat catttacgga agaagtcact tcatttaagg aacgtgagct tcatgaccgt
       61 tatgccgcta tttatatgga cgcaacctat atccctttaa agcggaaaac cgtcgccaag
      121 gaagccattc atatcgcagt tggcattcgc ccggacggtt caaaggaagt gttgagctat
      181 gcgattgcac caactgaatc tatcacgatt tgggaagaga ttttaataga cctccaggaa
      241 cgtggcgtga aaaacgtcct ccttttcatc acggatggct taaaggggat ggcaggggcc
      301 gtgcagcggt tctaccccaa agcccgtttt caacattgct gtgtgcacgt ttcccgtaat
      361 atcagtcaca aagtgcgtgt cgatgatcgt aaggaagtct gtgatgattt taaaatggtg
      421 tatcaagcgt catctaaaaa ggcggcattg gaagcacgtg gtgcttttgc ggaaaaatgg
      481 aaaaccagtt atccaaaaat ggtggaatcg atcctttcga acgattacct gctcactttc
      541 tatgattttc cattagccat acgcaagact atttactcta cgaacttgat tgaatccttt
      601 aataagcaaa tcaagaaata cagcc
//
LOCUS       DAXWOO010000353          600 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.364, whole genome shotgun sequence.
ACCESSION   DAXWOO010000353 DAXWOO010000000
VERSION     DAXWOO010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.364"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(66..150)
                     /locus_tag="ACGE70_003013"
     tRNA            complement(66..150)
                     /locus_tag="ACGE70_003013"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Leu,seq:caa)
     gene            complement(168..239)
                     /locus_tag="ACGE70_003014"
     tRNA            complement(168..239)
                     /locus_tag="ACGE70_003014"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(205..207),aa:Gln,seq:ttg)
     gene            complement(249..321)
                     /locus_tag="ACGE70_003015"
     tRNA            complement(249..321)
                     /locus_tag="ACGE70_003015"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(286..288),aa:His,seq:gtg)
     gene            complement(383..467)
                     /locus_tag="ACGE70_003016"
     tRNA            complement(383..467)
                     /locus_tag="ACGE70_003016"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(431..433),aa:Leu,seq:caa)
     gene            complement(488..558)
                     /locus_tag="ACGE70_003017"
     tRNA            complement(488..558)
                     /locus_tag="ACGE70_003017"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(524..526),aa:Cys,seq:gca)
BASE COUNT          185 a          145 c          129 g          141 t
ORIGIN      
        1 gtaattacat aaaaacaaaa aaaatatagc aactctctat aagaaagttg ctatattgat
       61 aattataccg gcggccgggg tcgaaccggc acgtcctcaa cggacactgg attttgagtc
      121 cagcgcgtct gccaattccg ccacgccggc aaatttaaaa ataataactg gggtagctgg
      181 attcgaacca acgcatgagg gagtcaaagt cccttgcctt accgcttggc tataccccaa
      241 gaataaaggg cgaatgatgg gaatcgaacc cacgaatgcc ggagccacaa tccggtgcgt
      301 taaccacttc gccacattcg ccatcataaa agatgttatt taatttctca ataaaaaact
      361 aagatgaaga catcttagtc aataccggcg gccggggtcg aaccggcacg ccctcaacgg
      421 gcactggatt ttgagtccag cgcgtctgcc aattccgcca cgccggcaag ttgatttatt
      481 agtactaagg cggtaaccgg atttgaaccg gtgatgaagg ttttgcagac ctctgcctta
      541 ccacttggct ataccgccat tatactatta tcaatctata aaataaagaa taaattatag
//
LOCUS       DAXWOO010000354          581 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.365, whole genome shotgun sequence.
ACCESSION   DAXWOO010000354 DAXWOO010000000
VERSION     DAXWOO010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.365"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            16..>581
                     /locus_tag="ACGE70_003018"
     CDS             16..>581
                     /locus_tag="ACGE70_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HFF8694546.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIK
                     YLNNLIEQDHRPVKRRNKFYRSLRTASTT"
BASE COUNT          203 a          101 c          115 g          162 t
ORIGIN      
        1 gcaggaggaa tgacgatgaa tcattttaaa ggaaagcaat ttcagcagga tgtgattatt
       61 gtagccgtgg gctactatct tcgttataac cttagctatc gtgaagttca agaaatctta
      121 tatgatcgtg gcattaacgt ttctcatacg acgatttatc gttgggtgca agaatatggc
      181 aaactactct atcaaatttg gaaaaagaaa aataaaaaat ccttttattc atggaaaatg
      241 gatgaaacgt acatcaaaat taaaggaaaa tggcattatt tgtatcgagc catcgatgca
      301 gatggtttaa ccttggatat ttggttacgt aaaaaacggg acacacaagc agcctatgct
      361 tttcttaagc ggttagtgaa gcagtttgat gaaccgaagg ttgtagtcac agataaagcc
      421 ccctctatta caagtgcctt taagaaacta aaagaatacg gcttttatca agggacagaa
      481 catcgtacca ttaaatacct gaataatttg attgaacaag accatcgtcc agtaaagaga
      541 cgcaataaat tctatcgaag tttacgcact gcctctacca c
//
LOCUS       DAXWOO010000355          571 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.366, whole genome shotgun sequence.
ACCESSION   DAXWOO010000355 DAXWOO010000000
VERSION     DAXWOO010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.366"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            260..>571
                     /locus_tag="ACGE70_003019"
     CDS             260..>571
                     /locus_tag="ACGE70_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFF8694547.1"
                     /translation="MYKAANDLFNRLKLEKKELILKYILDEFGRVGYEATSVRKIASK
                     ADLSVGSLYQYFNNKEGMLTVAIEHVFVILDEYVSTINDAPLKLKDRLEIMFEMILKL
                     GK"
BASE COUNT          211 a           62 c           93 g          205 t
ORIGIN      
        1 aatacaattt aaaattaggg tccataatat tttgtgttga tagaaaatcg acttcgattt
       61 tctatcaaca ctttttcttc gtttcaaatc aaaacaaaaa gagactcact tttggtaaaa
      121 tttaagatgt taatcatttt gtgtgtcggt taatttttat caatctaaga tttaattata
      181 gatcattaaa aatgagcggt cgtttttata tactgaatat ggtacactaa attaaaatac
      241 cttttttgag gagatcatta tgtataaagc agcgaacgat ttatttaatc gattaaaatt
      301 agaaaaaaag gaactcattt taaagtatat tttagatgag tttgggagag taggttatga
      361 agccacttct gttcgtaaaa ttgccagtaa agcagattta agtgtaggat cattatatca
      421 gtatttcaac aataaagaag gtatgttaac agtcgcgata gaacacgttt ttgttatttt
      481 agatgaatat gttagtacta taaacgacgc tcctttaaaa ctaaaagatc gtttagaaat
      541 aatgtttgaa atgattttaa aattaggaaa g
//
LOCUS       DAXWOO010000356          568 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.367, whole genome shotgun sequence.
ACCESSION   DAXWOO010000356 DAXWOO010000000
VERSION     DAXWOO010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.367"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..>568
                     /locus_tag="ACGE70_003020"
     CDS             <1..>568
                     /locus_tag="ACGE70_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFF8694548.1"
                     /translation="KGKGRFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVRE
                     IRALEKNIKELDKAIEQVIVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKL
                     AKYAGLSWKVNQSGNYQSQNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYK
                     EVPKHQHKRALVLTARKFTRLVDTLLRKH"
BASE COUNT          212 a          109 c          103 g          144 t
ORIGIN      
        1 aaaggaaaag gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt
       61 accatgagtt accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt
      121 ctcgtacgag aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa
      181 caagtaattg ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa
      241 gtctatgctg ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca
      301 aaattggcaa aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg
      361 caaaatacac ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct
      421 gccaactctg taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag
      481 gaggttccaa agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt
      541 ctggtggata cgctactacg taaacacc
//
LOCUS       DAXWOO010000357          558 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.368, whole genome shotgun sequence.
ACCESSION   DAXWOO010000357 DAXWOO010000000
VERSION     DAXWOO010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.368"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            55..>558
                     /locus_tag="ACGE70_003021"
     CDS             55..>558
                     /locus_tag="ACGE70_003021"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694549.1"
                     /translation="MFNQKKEKQFKYAIKKKKGGGGAASFVIGAVFLGIGLISYPTLS
                     FASETTEINQSSEIQSSLTNSVLKESNSEEKQNIDMSEDSSINNDLELDSKATVSGSE
                     NSFDETTNNLETTNLSEDSIEVQSESIIQNEDSTDNDAVQELEMNPHEDNGDDISVEN
                     QMTEATEE"
BASE COUNT          218 a           72 c          103 g          165 t
ORIGIN      
        1 cgattatttc gtctcttttt gatttttata ctgtaataaa taaggaggaa aattatgttt
       61 aatcaaaaga aagagaagca gttcaaatat gcaatcaaaa agaaaaaagg tggcggggga
      121 gcagcttcct ttgtcatagg tgcagtattt cttggaatag ggttaatttc ttatcctaca
      181 ttatcttttg ctagtgaaac aacagagata aatcaatcaa gtgaaatcca atctagtttg
      241 actaattctg ttctaaaaga aagtaattca gaagaaaaac aaaatattga tatgtcggaa
      301 gatagttcta taaataacga tctagaacta gattcgaaag caaccgtttc tggatcagaa
      361 aattcatttg atgaaactac taacaatcta gaaactacaa atttatctga agatagtatt
      421 gaagttcagt cagagagtat tattcaaaat gaagactcga ctgataatga tgctgttcaa
      481 gaattagaaa tgaatcctca tgaagataac ggtgatgata tttcagttga gaatcagatg
      541 acagaagcta cagaagaa
//
LOCUS       DAXWOO010000358          549 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.369, whole genome shotgun sequence.
ACCESSION   DAXWOO010000358 DAXWOO010000000
VERSION     DAXWOO010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.369"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            35..532
                     /locus_tag="ACGE70_003022"
     CDS             35..532
                     /locus_tag="ACGE70_003022"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8694550.1"
                     /translation="MNNQIALAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
BASE COUNT          200 a           78 c          103 g          168 t
ORIGIN      
        1 atttatagta tactatgtca aaaggaggag ttagatgaac aatcaaatcg cactagcgta
       61 tttagattat gatgatctag atgctttaca agttgtaagt gttgtcagtt tttatgaatc
      121 gtttatcgat ggcgcagatc cacttgatat gcaaggatat ttacagacac aattgacaac
      181 tgaaatattg gcagtagaat tggcacagtc aaccagtaaa tttattggta ttaaggatca
      241 tcaaatactc attggttata tgaaagtcaa tgatgaaaaa gatgctatag agattcaacg
      301 gctctatctg ctcaaagatt atcaaaacaa agggttaggg caacggttac tcgatgaagc
      361 gaatcgttat gctaaaacga aacaaaaacg ttatttacgt ttgactgttt atgaaaaaaa
      421 tcatgcaggt attcgttttt atgaacgtca tggatttaaa aaaatcggga tcaaacattt
      481 tcctttagga aaacaagatc gtatttgtcc gattttagaa aaagaaatct aaaaaaacgt
      541 cctcacttt
//
LOCUS       DAXWOO010000359          547 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.370, whole genome shotgun sequence.
ACCESSION   DAXWOO010000359 DAXWOO010000000
VERSION     DAXWOO010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..547
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.370"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(<1..413)
                     /locus_tag="ACGE70_003023"
     CDS             complement(<1..413)
                     /locus_tag="ACGE70_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFF8694551.1"
                     /translation="MHKISVIVPAYNVENYLENCLESISNQTHSNIEIIVVDDGSPDR
                     SGEIAEEYKKKDMRLKVIHQVNGGLSAARNSGLDSATGDFVCFIDSDDWIEPDYLELL
                     YFGTQTYGADISVLKIKKISSLEQLKEHTIAERKW"
BASE COUNT          167 a          110 c           61 g          209 t
ORIGIN      
        1 tcccattttc tctcagcaat tgtatgttct tttagttgtt ctaaacttga aatctttttt
       61 atttttagca ccgagatatc tgctccataa gtttgcgtac caaaatatag taactcaaga
      121 tagtctggtt ctatccaatc atcgctgtca ataaagcaca caaaatctcc tgttgcagaa
      181 tcaagaccag aattcctagc cgcacttaaa ccaccattca cttgatgtat cacttttaat
      241 cgcatatctt tctttttata ttcttctgca atttccccac ttcgatccgg ggaaccatca
      301 tcgactacta ttatttcaat atttgaatgt gtctgattgg aaatagattc taagcagttt
      361 tctaaatagt tttctacgtt atatgcgggt actataacac taattttatg cactctcttc
      421 acttcctaaa aatcatcttt tttattttta caaatgtatc attaccagta acaattttag
      481 tcttccgaag ttaataaaca taaaaaataa catatcgaaa aaaactttat ttttttagaa
      541 ctttttt
//
LOCUS       DAXWOO010000360          524 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.371, whole genome shotgun sequence.
ACCESSION   DAXWOO010000360 DAXWOO010000000
VERSION     DAXWOO010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.371"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(152..346)
                     /locus_tag="ACGE70_003024"
     CDS             complement(152..346)
                     /locus_tag="ACGE70_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694552.1"
                     /translation="MVHSTIGVVVEKSRDNLVFVTEIQTGRSFVVTDKAAKAYQSSDI
                     LTLNLTTKTFVDATEDYPFV"
BASE COUNT          163 a          110 c           79 g          172 t
ORIGIN      
        1 tgtgatagag aaataacaat ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat
       61 ttgattttga tcgggtctag tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc
      121 ttaaatttca tcttgactta tcaccattag actaaacaaa tggataatct tctgtcgcat
      181 cgacgaacgt cttcgtagtc agatttaacg ttaaaatatc gctactttga taagcttttg
      241 ccgctttgtc tgtgacgaca aaagatctgc ctgtttgaat ttctgtcaca aaaacgagat
      301 tgtctctgct tttttcaaca acaactccaa tcgttgaatg aaccatttct gcttcctccc
      361 ttctctttct tttgatataa tgtagtcgca aatcgcacca ctcaatgagt tggcggaaaa
      421 caattcattg aaaaaaagag tcctggacaa tcacgttcag gactctttta cttcttttat
      481 tcacttaaca actaacactc tgtgagtgaa atggactctt caat
//
LOCUS       DAXWOO010000361          520 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.372, whole genome shotgun sequence.
ACCESSION   DAXWOO010000361 DAXWOO010000000
VERSION     DAXWOO010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.372"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(<1..>520)
                     /locus_tag="ACGE70_003025"
     CDS             complement(<1..>520)
                     /locus_tag="ACGE70_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694553.1"
                     /translation="YVFEKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAF
                     SLLTFYPFFQLLRGEEINRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGR
                     YNYKLSVFSIISLAELIFSLTTPGNALRAAHEINKWFPEYKNFNFLNKLDLGISSFGK
                     PFFLDMNILFLLL"
BASE COUNT          202 a           81 c           65 g          172 t
ORIGIN      
        1 atagcaataa aaataagata ttcatgtcta aaaaaaacgg ctttccaaat gatgaaatac
       61 ctaaatctaa cttattaaga aagttgaaat ttttatattc aggaaaccat ttattaattt
      121 catgtgcagc acgtaatgca ttccctggag ttgtcaaaga gaaaattaat tctgctaagc
      181 ttattattga aaatactgat aacttataat tatatcgtcc attgacaatc aaatataaac
      241 taactatact tgttagaaca aaaaaacagg cgttgacttg ttcttgatta gttaaaaata
      301 tcaataaagg aatacttacc caatatattt ttctatttat ttcttctcct cttaagagtt
      361 gaaaaaacgg ataaaaagtt agtaaactaa atgctactgg ccactgatag ttcaaggtgg
      421 ttgctctcca accggtttcc cccattactg tcaatggtat taaacagtat atagagcaaa
      481 ccaataataa atttttaaca tttattttct caaaaacata
//
LOCUS       DAXWOO010000362          519 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.373, whole genome shotgun sequence.
ACCESSION   DAXWOO010000362 DAXWOO010000000
VERSION     DAXWOO010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.373"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(<1..267)
                     /locus_tag="ACGE70_003026"
     CDS             complement(<1..267)
                     /locus_tag="ACGE70_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HFF8694554.1"
                     /translation="MIGEKMMDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVL
                     SASIPILIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQ"
BASE COUNT          187 a           73 c           58 g          201 t
ORIGIN      
        1 ctgtacttta tcttgataat taaatgcaga catcccactt tgtataattg taattattgc
       61 tgaagcaatt gaaacaatca atagtaaaga cctgtgttcg gaagcctgat caattaatat
      121 tgggatacta gctgataaga ctatttttat aatatttccg ataccaatta ctctattata
      181 aaatcgaatt tcttttttta atctatcgat tgtagaatca atagattcga taaacgcttt
      241 ttgatccatc attttctctc caatcatttt attaaaatca ttaacaaaaa tattataata
      301 ggatttaagt aagttctaac taatcacata taaaaataaa aaaacatttg tattttatca
      361 aagttttatt atacttgtca atttgtgtac atcctaaaat ataagctatt tgtttacttc
      421 tgtagaatat atactttgtt gttagataag ttgcacatgt ttatagaatc taaataataa
      481 atttttatgt accgcccctc aatggataaa gtccatata
//
LOCUS       DAXWOO010000363          518 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.374, whole genome shotgun sequence.
ACCESSION   DAXWOO010000363 DAXWOO010000000
VERSION     DAXWOO010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..518
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.374"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(<1..221)
                     /locus_tag="ACGE70_003027"
     CDS             complement(<1..221)
                     /locus_tag="ACGE70_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFF8694555.1"
                     /translation="MSTNKKDNKKEEIIFTAMKHFSKFGYEKTKLLDIANEAKTSTTT
                     VYSFFKTKHDLYEAAVEYDLNIINFELER"
     gene            complement(226..>518)
                     /locus_tag="ACGE70_003028"
     CDS             complement(226..>518)
                     /locus_tag="ACGE70_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFF8694556.1"
                     /translation="KKMITEWVKQFYNNRSFFDVYWSTVKELQNEKEISTKIDPVIYT
                     FIIDTMMLSGELLQNTQYQEIKAKVFLEDSIKSSLILKENVIETLTVLAKGQ"
BASE COUNT          162 a           75 c           56 g          225 t
ORIGIN      
        1 attctttcaa gttcaaagtt tattatattt aaatcatact ctactgcagc ctcatacaaa
       61 tcatgttttg ttttaaaaaa actatataca gtagtagtac tagttttagc ttcattagca
      121 atatctaaaa gctttgtttt ttcataacca aatttagaaa aatgtttcat tgcggtaaat
      181 ataatttctt cttttttatt atctttcttg tttgttgaca taaatttatt gcccctttgc
      241 aagtacagtt agcgtctcga taacattttc ttttaatatt aatgaacttt ttatagagtc
      301 ttctagaaaa actttggctt ttatttcttg atattgtgta ttttgaagca gctctccaga
      361 taacatcatt gtatcaataa taaatgtata gataactgga tcaattttag tactaatttc
      421 tttttcattt tgtagttctt taacagtact ccagtaaacg tcaaaaaaac ttctattatt
      481 atagaattgt ttaacccatt cagttatcat ttttttat
//
LOCUS       DAXWOO010000364          510 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.376, whole genome shotgun sequence.
ACCESSION   DAXWOO010000364 DAXWOO010000000
VERSION     DAXWOO010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.376"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..>510
                     /locus_tag="ACGE70_003029"
     CDS             <1..>510
                     /locus_tag="ACGE70_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFF8694557.1"
                     /translation="DMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNE
                     LRKVGQKSQAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGF
                     STSLKEAYPYFHELVEAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITNA
                     MIYPYTNGK"
BASE COUNT          170 a          109 c           97 g          134 t
ORIGIN      
        1 agacatgaac gccgcctact tccagcttac caaacgtatt ctgccaaatg cgaaagtagt
       61 gattgatcgg tttcatatta tcaaacacat gaatcaagcc tttaatgagt tgcgtatccg
      121 tgaaatgaat gaacttcgta aagtaggaca gaaaagtcag gcagaaaaac tgaaaaagaa
      181 ctggcgcttt ttgctaaaaa atcgtgcaaa catcaaccat tatgaataca aaacatggaa
      241 aagttttcga gcaccaaaat acccatttct tacggaagcg atgatgattg atcgattgct
      301 tggcttttct acgtccctga aggaggcgta tccctatttt catgaattag ttgaagcctt
      361 tcgagacaaa gaccctgact tatttttctc cttattggca gaacttcccg aaacgttaga
      421 tgacggcttt cgggaaaagc tccaaaacct tctgacctat gaagaaggca tcaccaacgc
      481 gatgatctat ccttacacca atggaaaaat
//
LOCUS       DAXWOO010000365          510 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.377, whole genome shotgun sequence.
ACCESSION   DAXWOO010000365 DAXWOO010000000
VERSION     DAXWOO010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.377"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            259..477
                     /locus_tag="ACGE70_003030"
     CDS             259..477
                     /locus_tag="ACGE70_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694558.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT          161 a           82 c           85 g          182 t
ORIGIN      
        1 atcgttattt attgatatta atctcatcca aaaaaatgaa agcgcatact tttatgagtg
       61 attgaatgtg attttaatac atataccaat taaaagaaaa ctttttaaaa agagtttgag
      121 acgttggtct caaactcttt tttcgcccgg gttcttcttc tttcaaattt ttatcacttc
      181 cctctttaca aaccgaacaa atattcgtat actaaatata cgaacaattg ttcgcataaa
      241 ttaaagaggt gagaattcat gaaatcaatg agacgtcttg gcttagtatt aactgttttg
      301 tttttaggta ttattattgg taatttcggc atacgtccag ctttacttgt gtttgttccg
      361 ttatttactt tgtggttcat gttgtgggat gaaaagaagt accgtcaatc tgagcggcgc
      421 agaattcatg aagatcattt ctatcatgag ccctactaca gatccaggca atcataataa
      481 ggattgactg ctaattattc aaaagacaag
//
LOCUS       DAXWOO010000366          508 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.378, whole genome shotgun sequence.
ACCESSION   DAXWOO010000366 DAXWOO010000000
VERSION     DAXWOO010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.378"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
BASE COUNT          227 a           70 c           81 g          130 t
ORIGIN      
        1 attccttagg cagcttttgc tgtgtagaaa ggaataaata ataacgaaag ttcaaaagct
       61 aatgccgact attctagaca ttaaagaaga cgaaaacgaa cgataaagaa agaaaagtta
      121 tgaataatcg tttattgaaa aaaacttgaa aaaatacaaa aaattcgttg accaagctaa
      181 atttagttgg tatattatta cttgtccaaa agacagaaga aaatatttat tcaaaaacat
      241 aacaaaaaag ttattgacat gttactaacg acatgataag ataactgagt cgttaaaaaa
      301 gaaccacttg cgaagcttga ttctatcgaa aaaaacttca aaaaagttct tgacaaataa
      361 caaacgataa gttatgataa atgagttgcc gaaagatttc gaaaaacgaa atcgaaaaaa
      421 acttttaaaa aagagttgac aatcgcttgt ctctctgata tgatataaaa gttgctacgg
      481 cagccaaaaa tgaatcggga aacactct
//
LOCUS       DAXWOO010000367          504 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.379, whole genome shotgun sequence.
ACCESSION   DAXWOO010000367 DAXWOO010000000
VERSION     DAXWOO010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.379"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(72..203)
                     /locus_tag="ACGE70_003031"
     CDS             complement(72..203)
                     /locus_tag="ACGE70_003031"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694559.1"
                     /translation="MQSAKACGIAFGLAKWGAKNIENFQSVDIIIDSPQEILDLLEK"
     gene            complement(176..298)
                     /locus_tag="ACGE70_003032"
     CDS             complement(176..298)
                     /locus_tag="ACGE70_003032"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694560.1"
                     /translation="MHKPFPDPIQKAIDELGIKNKKRFILVIHYMTCKVRKLVG"
BASE COUNT          172 a           93 c           68 g          171 t
ORIGIN      
        1 gcaaaaattt caatctttaa atggcgaaaa acaaagagcg tgagacccta tccttatctc
       61 acgctctttg tctatttttc taacaaatca agtatttctt gaggagaatc aataattata
      121 tctacacttt gaaaattttc tatatttttg gctccccatt tagctaaacc aaatgctatc
      181 ccacaagctt tcgcactttg catgtcatat aatgaatcac caatataaat cgtttcttat
      241 tttttatgcc caattcatca attgcctttt gtatggggtc cggaaaaggt ttatgcaact
      301 tagtatctga agctgtaatt tgtatatcaa aatatagatt taacccgtgg tgctagttaa
      361 ttttttccta gaggaaaatg tctaaacatg ttggtaaatc aacaaaaaaa cgcatatagt
      421 agtctcaaaa ctcccaagaa atgaggacta catatgcgtt catcaaaata tactgaacat
      481 tatacagata cttttataac attc
//
LOCUS       DAXWOO010000368          481 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.381, whole genome shotgun sequence.
ACCESSION   DAXWOO010000368 DAXWOO010000000
VERSION     DAXWOO010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.381"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            96..>481
                     /locus_tag="ACGE70_003033"
     CDS             96..>481
                     /locus_tag="ACGE70_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFF8694561.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHH"
BASE COUNT          175 a           67 c           80 g          159 t
ORIGIN      
        1 attttgtgta aatagatatt tagggtagaa aaaaagaaag tccattctgt aaaattaaag
       61 ttaccacaac cgaaatttta taggaggact ttctcatgcc caattttact acagaaatta
      121 tggaaacact aatcaataaa ggtgatttag atgaattatt tcgatgtcat ttagaattag
      181 ctgttaattc attattgcag gctgaattaa cagcttttct tgattatgaa aaatatgatc
      241 gtgctgggtt caattcaggt aattcccgta atgggaatta ttcacgttca ttcagaacag
      301 agtatggaga attaaatttg gtaattccta gagatagaaa tggtaagttt tctcaacaaa
      361 ctttacctgc ttataaaaga accaatgact ctttagaaac cactattatt cagctatttc
      421 aaaaaggaat tactatgtct gaaatctctg aattgattga aaaaatgtac gggcatcact
      481 a
//
LOCUS       DAXWOO010000369          464 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.382, whole genome shotgun sequence.
ACCESSION   DAXWOO010000369 DAXWOO010000000
VERSION     DAXWOO010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.382"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..>464
                     /locus_tag="ACGE70_003034"
     rRNA            <1..>464
                     /locus_tag="ACGE70_003034"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          141 a          103 c          131 g           89 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagt
//
LOCUS       DAXWOO010000370          462 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.383, whole genome shotgun sequence.
ACCESSION   DAXWOO010000370 DAXWOO010000000
VERSION     DAXWOO010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.383"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(<1..430)
                     /locus_tag="ACGE70_003035"
     CDS             complement(<1..430)
                     /locus_tag="ACGE70_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFF8694562.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          132 a           92 c           59 g          179 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tc
//
LOCUS       DAXWOO010000371          434 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.384, whole genome shotgun sequence.
ACCESSION   DAXWOO010000371 DAXWOO010000000
VERSION     DAXWOO010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.384"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(<1..>434)
                     /gene="scm"
                     /locus_tag="ACGE70_003036"
     CDS             complement(<1..>434)
                     /gene="scm"
                     /locus_tag="ACGE70_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFF8694563.1"
                     /translation="VNIDAEVLTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYN
                     DRSNHETEDFSDANISWNFGNEMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNV
                     ILDAATNRWDPNEIFISDNGTDTTDTSDSTDTSTTSDSSDSS"
BASE COUNT          118 a           77 c           86 g          153 t
ORIGIN      
        1 tgatgaatct gagctatcac ttgtggtaga tgtatctgta ctgtcgcttg tgtctgtcgt
       61 atctgtacca ttgtcgctga taaatatttc gttaggatcc caacgattgg tagctgcatc
      121 taaaatcaca tttgttccta taatcgttcc gtcttggttt tgagtaatgc ggatagtagc
      181 attaggcgca aggattgttc cttgaaaagg agcactgatt gtcaaatcag tcatctcatt
      241 tccaaaattc caagagatat tcgcatccga aaaatcttct gtttcatgat ttgaacgatc
      301 attgtatttg attggtgact gtacgttcaa tgcagaagaa ctgttgatga cattaaatac
      361 aactacttga tcatttggat taatgatttg aagtggcgta ttcatcgtta acacttctgc
      421 atcgatattt acca
//
LOCUS       DAXWOO010000372          429 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.385, whole genome shotgun sequence.
ACCESSION   DAXWOO010000372 DAXWOO010000000
VERSION     DAXWOO010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.385"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..96
                     /locus_tag="ACGE70_003037"
     CDS             <1..96
                     /locus_tag="ACGE70_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8694564.1"
                     /translation="PRACHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            97..>429
                     /locus_tag="ACGE70_003038"
     CDS             97..>429
                     /locus_tag="ACGE70_003038"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8694565.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKG"
BASE COUNT          180 a           28 c           81 g          140 t
ORIGIN      
        1 cctagagctt gccatgtata tgaaaaatta ggtggtaaaa atgattataa agatgaaata
       61 gtatatgtat atgattatga aaaaggtgat aaataaatga atatagttga aaatgaaata
      121 tgtataagaa ctttaataga tgatgatttt cctttgatgt taaaatggtt aactgatgaa
      181 agagtattag aattttatga tggtagagat aaaaaatata cattagaatc attaaaaaaa
      241 cattatacag agccttggga agatgaagtt tttagagtaa ttattgaata taacaatgtt
      301 cctattggat atggacaaat atataaaatg tatgatgagt tatatactga ttatcattat
      361 ccaaaaactg atgagatagt ctatggtatg gatcaattta taggagagcc aaattattgg
      421 agtaaagga
//
LOCUS       DAXWOO010000373          427 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.386, whole genome shotgun sequence.
ACCESSION   DAXWOO010000373 DAXWOO010000000
VERSION     DAXWOO010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.386"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(<1..>427)
                     /locus_tag="ACGE70_003039"
     CDS             complement(<1..>427)
                     /locus_tag="ACGE70_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFF8694566.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDEL"
BASE COUNT          125 a           76 c           87 g          139 t
ORIGIN      
        1 agttcatcca atgtgaaatc ttccgtcatg agtgaaatga gggttgcact aaaaagactc
       61 gtcgattcgt ctcgcatttc tcgagcaagc gtattacatt tgtacgttag attttcaaga
      121 aaatgttgct ttgtacgaat caattgctta ataagttgat agcgagtacg tgttaaacgc
      181 tgtaaggcca tgtatttctc ttctttgata ggggaagttg tgaatcgttg aatacgcaag
      241 aaatcagcga ttcttaatgc atcgtttcga tcagttttat tctcatcaaa catgcgacta
      301 aattgtttca cccgaaaagg attttcaatc gttacaagtg tgttgagctt ctttagtttc
      361 tcatcttcat gaaaaaacat ggaaggatgg aagctgtaca tggacgttga ctccatgcca
      421 atcacaa
//
LOCUS       DAXWOO010000374          421 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.387, whole genome shotgun sequence.
ACCESSION   DAXWOO010000374 DAXWOO010000000
VERSION     DAXWOO010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.387"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..323
                     /locus_tag="ACGE70_003040"
     CDS             <1..323
                     /locus_tag="ACGE70_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFF8694567.1"
                     /translation="EALDHIDFMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIY
                     STNLIEGFNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRIHKGFKEIQDTL
                     ESMI"
BASE COUNT          156 a           76 c           59 g          130 t
ORIGIN      
        1 aagaggcatt ggatcacatc gactttatga ctaggaaatg gaaaaagcag tatccaagag
       61 tcgtcaattt actcttgaat cctgccctat taacctttta taatttccct cacgccatca
      121 gacgaacaat ttattcgacg aacctgattg aaggctttaa taagcagcta aaacgatata
      181 ctcgaagaaa agaacaattc cctaatgaag aatctctaga gagattcttc atttctcaat
      241 ttaatcaata taaccaaaaa tttttaggta gaattcacaa gggatttaaa gaaattcagg
      301 atacattaga gtcgatgatt taactgtaat taacagaatg gattttccat ttacatataa
      361 ttcttgacgc taccataatc taaataacca tcatattcat ctactactta tataattgag
      421 t
//
LOCUS       DAXWOO010000375          421 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.388, whole genome shotgun sequence.
ACCESSION   DAXWOO010000375 DAXWOO010000000
VERSION     DAXWOO010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.388"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..>421
                     /locus_tag="ACGE70_003041"
     CDS             <1..>421
                     /locus_tag="ACGE70_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HFF8694568.1"
                     /translation="LLNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKL
                     QELELHYSYLVKITEEKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKL
                     IAYMQKANADVEKELYANKIQELEEKLKELEVIRENVL"
BASE COUNT          200 a           45 c           65 g          111 t
ORIGIN      
        1 attactcaat aaactaacta tttctaatat agactcgact agggaaaaaa tcataaaaat
       61 atttgataaa attaataatt tattcaagaa tgacaatgct gaattaagga agggtctttt
      121 agagtttaaa cttcaagaac tagaacttca ttactcttat ttagtgaaaa ttaccgaaga
      181 aaaagaacaa caaaaagcaa taaaagaaca gatgatagaa gaggaaaaag ttcgtagaga
      241 aattgatcgt gaaaagaaac gtattgataa agaacaacgt caattcaatt ctgaaatttc
      301 caaattaatc gcatatatgc aaaaagctaa tgcagatgtg gaaaaagaat tatatgcaaa
      361 taaaattcaa gaactggaag aaaaattaaa agaattagaa gttatacgtg aaaatgtatt
      421 a
//
LOCUS       DAXWOO010000376          418 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.389, whole genome shotgun sequence.
ACCESSION   DAXWOO010000376 DAXWOO010000000
VERSION     DAXWOO010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.389"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
BASE COUNT          151 a           66 c           59 g          142 t
ORIGIN      
        1 tgataaagaa gtcaaggtgt ctttttgatt ggtgatggca gattctgcat aattgatccg
       61 ttgctgatgt tgccgctgat tattgatatg gtaaccaaac aaacagatcg caatagcacc
      121 tgcacctacc gccacagcag tcaatgactt gacgaatggc ttttccatag attcactcct
      181 aagtattgat gatagataaa ttaagatata attcacatac ataaaaatga cagcttttta
      241 ttttaaaaaa cataattaaa ttaatatatt cacagaaata tttgtaaaca agttttccct
      301 atataatcat accacaaatt gttttcttga ctagggcaat ttacgattta aaagtaaata
      361 aaacattaat aagataacaa ttaatcgatt tcatttttat ttccttaatt ttcatagg
//
LOCUS       DAXWOO010000377          415 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.390, whole genome shotgun sequence.
ACCESSION   DAXWOO010000377 DAXWOO010000000
VERSION     DAXWOO010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.390"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(<1..339)
                     /locus_tag="ACGE70_003042"
     CDS             complement(<1..339)
                     /locus_tag="ACGE70_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8694569.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DAXWOO010000378          405 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.391, whole genome shotgun sequence.
ACCESSION   DAXWOO010000378 DAXWOO010000000
VERSION     DAXWOO010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.391"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            121..345
                     /locus_tag="ACGE70_003043"
                     /pseudo
     CDS             121..345
                     /locus_tag="ACGE70_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
BASE COUNT          137 a           59 c           71 g          138 t
ORIGIN      
        1 caatatcgat cagttttgag gaaatgaaat tctagatatt ttttgctaca tagagttact
       61 ttcaatattt tttaacgtaa ttaggcagaa gtcactcatc ctctataggt ttgtgatgaa
      121 attgccattg atccatttaa gtaagagcaa aaaactggag gtttatcagg taattggtct
      181 aatggattta agtatgcggt caccgtgtat agagttgctt atgagattca agaaaataaa
      241 gttattccag tattgctata tggaacacat aacaattttt atgaattagc cgagaacact
      301 cgcaacttta ttcgtgtaga tgaatcggct tcggttgagt tataacttta gctatatcaa
      361 tcaaccgacc gacatatgtt aaatctatat ataataaaaa tacct
//
LOCUS       DAXWOO010000379          401 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.392, whole genome shotgun sequence.
ACCESSION   DAXWOO010000379 DAXWOO010000000
VERSION     DAXWOO010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.392"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..380
                     /locus_tag="ACGE70_003044"
     CDS             <1..380
                     /locus_tag="ACGE70_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFF8694570.1"
                     /translation="EICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSLIENQ
                     DLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFDTYNQ
                     KFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          153 a           72 c           63 g          113 t
ORIGIN      
        1 aagaaatctg tgatgacttt aaggctgttt atcaagctaa ctcaaaagaa gaagcgaata
       61 ccttcttatc cggcatgatt gagaaatgga agaaaaacta tcctaaagtg acgcagtcac
      121 tcatagaaaa ccaagactta ttaacttttt atgattttcc acctagcatt cgtagaacca
      181 tttactcaac caatctaatc gagtctttca ataagcaaat taaaagatac agccgtagaa
      241 aagagcagtt tcaaaatgaa gaatcactag aacgctttct agtcagcatt tttgatacat
      301 acaatcaaaa atttctaaac agaagccata aaggttttca acaggtaacc gatacattag
      361 tttcaatgtt tactgagtaa ctaattattt tgcaggagga c
//
LOCUS       DAXWOO010000380          392 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.393, whole genome shotgun sequence.
ACCESSION   DAXWOO010000380 DAXWOO010000000
VERSION     DAXWOO010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.393"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(110..182)
                     /locus_tag="ACGE70_003045"
     tRNA            complement(110..182)
                     /locus_tag="ACGE70_003045"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(147..149),aa:Thr,seq:ggt)
BASE COUNT          156 a           60 c           56 g          120 t
ORIGIN      
        1 atatagactt tctttgtact attaaaatat aaacattcat ttcttacgtc ataaaataaa
       61 aaaacagcgc agacacattg ttaaaccaac atgttgcact gtttaaggaa gccacttgcc
      121 gggcttgaac cggcgacctc ttccttacca tggaagcgct ctaccagctg agctaaagcg
      181 gcattatcat taatacaaat aataggataa cgaatattaa tgtatttgtc aacagaattt
      241 tttcgaaaaa atgtgtttct ctaataggaa aacagatttt agaaaaaata aaaataacaa
      301 aaaatgcgaa cggtttcttt gataatataa taaaaaaatt caaaaaaagt attgcgttat
      361 ttagaagaaa ttgatattat attcatgtac tt
//
LOCUS       DAXWOO010000381          389 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.394, whole genome shotgun sequence.
ACCESSION   DAXWOO010000381 DAXWOO010000000
VERSION     DAXWOO010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.394"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..>389
                     /locus_tag="ACGE70_003046"
     CDS             <1..>389
                     /locus_tag="ACGE70_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFF8694571.1"
                     /translation="IFKFANRRYGLKYNPVNGVSLARKPKTNEEIKKEKDGYLSKEEV
                     QMIASKQKSSHQQSRYSLITKFLFLTGLRYGELISLTESDYDGNKITVSGTYDYELKI
                     KTTTKNTGSYRTIELSSNAKEIIDQLI"
BASE COUNT          159 a           48 c           59 g          123 t
ORIGIN      
        1 gatttttaaa ttcgccaacc gtcgatatgg attaaaatat aatcccgtaa atggggtttc
       61 tttagcaaga aaacctaaga caaacgaaga aataaaaaaa gaaaaagatg gttatttatc
      121 aaaagaagaa gttcaaatga ttgctagtaa acaaaaaagt tcacatcaac agtcacgtta
      181 ttctttaatt actaaatttt tgtttttaac tggactacgt tatggtgaat tgatatcttt
      241 gactgaatct gattatgatg gtaataaaat tacggtttct ggtacttatg attacgagtt
      301 aaaaattaaa acaacaacta aaaatacagg ttcttataga accattgaat tatcaagtaa
      361 tgctaaagaa atcatagatc aattaattg
//
LOCUS       DAXWOO010000382          384 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.395, whole genome shotgun sequence.
ACCESSION   DAXWOO010000382 DAXWOO010000000
VERSION     DAXWOO010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.395"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
BASE COUNT          170 a           36 c           39 g          139 t
ORIGIN      
        1 aaaaaataat tcaaaattta aatgttttaa tattacaaat aaaatcatgt tatcgtaaaa
       61 tggtataaaa aagctaaata ttctattttt gttattttat tagcagggaa atttaaatag
      121 tttacaagaa tccgtagaaa tatattggtt ttgccttcag attaatactg gatcatatta
      181 atctgaatat aatatatgaa ctaataaata gcttaacctc gctattttgt tttttctgat
      241 cactatctat acagcattag taaatagaat tttaaaaata aaaaaattaa gacaaaaaaa
      301 gtatcaacat aaacatatac atatatatgt ttatgttgta gaatataaaa gcaaatataa
      361 atataaacac gtttatgtat ataa
//
LOCUS       DAXWOO010000383          382 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.396, whole genome shotgun sequence.
ACCESSION   DAXWOO010000383 DAXWOO010000000
VERSION     DAXWOO010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.396"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            138..>382
                     /locus_tag="ACGE70_003047"
     CDS             138..>382
                     /locus_tag="ACGE70_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002384409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFF8694572.1"
                     /translation="MEVIIMNVGFIGAGKVGCSFGKYFQEHKIQVTGFYSKSEDSSLA
                     ASNFTSSKQYLNLRELVDENDTIFITTPDGQIQEVWQ"
BASE COUNT          132 a           43 c           72 g          135 t
ORIGIN      
        1 tatcttttat tttaaaatat gggatattat gataaattaa ctatggatga tatttgaaaa
       61 aaacatactg tatatttgat ttaattttta tttttaagct catatcgtaa gaatgggact
      121 tgtagtctca cttctttatg gaggtgataa ttatgaacgt tggttttatt ggagccggca
      181 aggtaggttg ttcattcggg aagtatttcc aggaacataa aatacaagta acaggatttt
      241 atagtaaaag cgaagactcg tcattagcag cttcgaattt tacatctagt aaacaatatt
      301 taaacttaag agaattagta gatgaaaatg atactatttt cattacgact cctgatggtc
      361 aaattcagga ggtatggcaa ga
//
LOCUS       DAXWOO010000384          364 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.397, whole genome shotgun sequence.
ACCESSION   DAXWOO010000384 DAXWOO010000000
VERSION     DAXWOO010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.397"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
BASE COUNT          143 a           53 c           67 g          101 t
ORIGIN      
        1 ctgttattca aatcatttta tcaaaactac atagtaaatg ttaaaaagca acatgacata
       61 atatatagaa ggctttacag tatatatggg agcttactaa aaaagaatgg agtaagagtt
      121 aacacaatgg gccattagct gaagttttaa gcacttcaag gtatgaaaca gaatggcctg
      181 taatactaca tgattattta gactgctcaa actgctatca gtctggtgga atgaataaag
      241 tattatacta aaagcagatc caactacaac gtaacaggaa accagttgta tcaggaaaag
      301 tgatagtaca tggagaagaa aaggccccct ttgtatgagt cagtacatat cttatttata
      361 gata
//
LOCUS       DAXWOO010000385          359 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.398, whole genome shotgun sequence.
ACCESSION   DAXWOO010000385 DAXWOO010000000
VERSION     DAXWOO010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.398"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..>359
                     /locus_tag="ACGE70_003048"
     CDS             <1..>359
                     /locus_tag="ACGE70_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFF8694573.1"
                     /translation="PDNYPDFNSEFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKP
                     NGRKALDIETALNQWFSRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTPS
                     QRPLNENSNGILRRNGL"
BASE COUNT          109 a           72 c           63 g          115 t
ORIGIN      
        1 gacctgataa ttatcctgat ttcaattctg agtttggtca ccttgaaggt gatacgatcg
       61 ttggcattca tcataaaagt gccgtcatta ctttagttga aagattatct aaagtcatta
      121 tcacgattaa acccaacggc cgtaaggcat tagatattga aactgccctt aatcaatggt
      181 tttctcgctt ccctaaaaac ttctttaaat ctattacgtt tgactgtgga aaagaatttt
      241 ctaactggaa agccattagt aaccaacatg atattgatat atattttgcg gaccctggaa
      301 caccttctca acgcccatta aacgagaatt ctaacgggat tctgcgtcgt aatggactg
//
LOCUS       DAXWOO010000386          354 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.399, whole genome shotgun sequence.
ACCESSION   DAXWOO010000386 DAXWOO010000000
VERSION     DAXWOO010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.399"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(<1..224)
                     /locus_tag="ACGE70_003049"
     CDS             complement(<1..224)
                     /locus_tag="ACGE70_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFF8694574.1"
                     /translation="MRKRLKLLLILVLFFVFFPLAHTLITMQSVVSLPFYLLPFTSYP
                     YIGNSMSQFLFWVSVCLLILLFIILIIVLF"
BASE COUNT          149 a           50 c           41 g          114 t
ORIGIN      
        1 tagaataaca cgataattag aataataaac aatagtatta ataaacatac actaacccaa
       61 aacaaaaatt gagacatgct attaccaata tacggatagc ttgtaaatgg aagtaggtaa
      121 aatggtaaac taacaacact ttgcattgta attaatgtat gtgctaaagg aaaaaataca
      181 aaaaataaaa caaggattaa taataatttt aatctttttc gcataataca cctctttatc
      241 tacgttttga acaaattcgc ttactttatc attccttgaa aatttacgat aaaatatata
      301 tctattaaaa aatctccaaa ggttgtggtg ctttgatttc tataatctat gctc
//
LOCUS       DAXWOO010000387          353 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.400, whole genome shotgun sequence.
ACCESSION   DAXWOO010000387 DAXWOO010000000
VERSION     DAXWOO010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.400"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..191
                     /locus_tag="ACGE70_003050"
     CDS             <1..191
                     /locus_tag="ACGE70_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFF8694575.1"
                     /translation="LKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKATTC
                     IAGMSIGGFLGGAIPGKC"
     gene            193..>353
                     /locus_tag="ACGE70_003051"
     CDS             193..>353
                     /locus_tag="ACGE70_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFF8694576.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINR"
BASE COUNT          137 a           39 c           65 g          112 t
ORIGIN      
        1 atttaaaaat tttgtctatt aaagagacac aacttatcta tgggggtacc actcatagtg
       61 gaaaatatta tggaaatgga gtgtattgca ctaaaaataa atgtacggtc gattgggcca
      121 aggcaactac ttgtattgca ggaatgtcta taggtggttt tttaggtgga gcaattccag
      181 ggaagtgcta aaatgaaaaa aaatgctaag caaattgttc atgaattata taatgatata
      241 tctataagta aagatcctaa atattctgat attcttgagg ttttacaaaa ggtatattta
      301 aaattagaaa aacaaaaata tgaattagat cccagtcctt taataaatag att
//
LOCUS       DAXWOO010000388          339 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.401, whole genome shotgun sequence.
ACCESSION   DAXWOO010000388 DAXWOO010000000
VERSION     DAXWOO010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.401"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(100..>339)
                     /locus_tag="ACGE70_003052"
     CDS             complement(100..>339)
                     /locus_tag="ACGE70_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694577.1"
                     /translation="YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRM
                     LEKRKCADYIPNETITKTDMALMNREAEKIINKLT"
BASE COUNT          106 a           62 c           42 g          129 t
ORIGIN      
        1 cgatcatcgg cgttatgact atataaagta tactttcgtt gatccagaaa gatacaaaga
       61 ttaaataaaa aaataacaaa caaactcgct tgaaattcat taggtgagtt tgtttattat
      121 tttttctgct tctcgattca tcagcgccat atctgttttc gtaatggttt cattaggaat
      181 atagtcagca cattttcttt tctctaacat tctataccca ctattatgta ttcctaaggt
      241 attcaattcc ttaactgttg aaaacaaatt ccaaacggtt tggtggcttc cttttccgct
      301 acttccatta taaataatat cataattttc tttcaaata
//
LOCUS       DAXWOO010000389          337 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.402, whole genome shotgun sequence.
ACCESSION   DAXWOO010000389 DAXWOO010000000
VERSION     DAXWOO010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.402"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
BASE COUNT          135 a           34 c           58 g          110 t
ORIGIN      
        1 aatagggaat tttctattgc aattttataa agagattgct atattattat ttgttctaaa
       61 tgattccttt aaggtgtcac taaaaattat aaaaaatagt gttgacgcac ttttgatgtc
      121 atgttataat aacgaagtcg tttggatgag atagctaata aagcttgatt ttatcgaaaa
      181 aaacttcgaa aaagttattg acaaataaca ataaagatgg tatgataatc aagtcgctgc
      241 taatgagtgg caaagacgtt tagaaaaaat aaacaaagaa gttgttgaca ttctcttctg
      301 aatctgataa gattaaagag ttgttaaaac aaaaaga
//
LOCUS       DAXWOO010000390          333 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.403, whole genome shotgun sequence.
ACCESSION   DAXWOO010000390 DAXWOO010000000
VERSION     DAXWOO010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.403"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..134
                     /locus_tag="ACGE70_003053"
     CDS             <1..134
                     /locus_tag="ACGE70_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFF8694578.1"
                     /translation="VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
BASE COUNT          125 a           54 c           40 g          114 t
ORIGIN      
        1 aagtgaatca gacatttatt tccagtgtca gcaatcaacg taatcatatt ccaagaaaat
       61 cattgaatta cagaacacca atcgaaatat ttttgagcta tgtacaagaa gcattttatt
      121 ctagcttaat ttgacaaatc ataactttaa attacggatc atgatttggc atggggaatc
      181 attactaata aaataaaaaa atgaacagaa agaaaacagt tctttctgtt caccaacttc
      241 atttgacaaa tatcctattt agcttatttt atagcttttt atatacactt ttttaaaaag
      301 tgatttttcc acatgaattc ccatgagcaa ttc
//
LOCUS       DAXWOO010000391          327 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.404, whole genome shotgun sequence.
ACCESSION   DAXWOO010000391 DAXWOO010000000
VERSION     DAXWOO010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.404"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
BASE COUNT          118 a           56 c           81 g           72 t
ORIGIN      
        1 tgtaaataaa taaaatgtct gacggatatt gaatagcaat aaataacaat aattcagacg
       61 aatgcggtga gtgaagtagc ttgctacgaa cgaacaggca gaatgaaacg gcagtttcat
      121 tagagcaagt cgcttgcgac gtagcgttac cgctgatgta actggcaagt tacatagaga
      181 gagtttgctt gcaaacgaac gttacctatt tgaatagaaa taaaaaataa cagattagtg
      241 aattttgaat aggagccaca actcaaccaa ccgcttgcgg tgggtgagtc ccacggagga
      301 aaagacgaac gaacggtcgc gtagcga
//
LOCUS       DAXWOO010000392          327 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.405, whole genome shotgun sequence.
ACCESSION   DAXWOO010000392 DAXWOO010000000
VERSION     DAXWOO010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.405"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..>327
                     /locus_tag="ACGE70_003054"
     CDS             <1..>327
                     /locus_tag="ACGE70_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFF8694579.1"
                     /translation="YVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANA
                     NKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPE
                     SGYYVKG"
BASE COUNT          122 a           51 c           74 g           80 t
ORIGIN      
        1 ttatgttcgc cgatttagag aaacgacggt gtttgaggat atttttgcct atattttaga
       61 acaagcagtt aaagcaggtt ttgtaacaga ggataatttg tatctggatt ctacccacat
      121 caaagctaac gccaataaac ataagtttac gaaagaaatg acccatgatg aagcgaaagc
      181 ctatcaggat gaattagaag atgagatcaa tcgtcaacga atagaggctg gaaaacgccc
      241 ttttactttg gacttagaaa aggaagtgaa gctgaaagaa agaaaaataa gtaaagctga
      301 tccggaaagc ggctactacg tcaaagg
//
LOCUS       DAXWOO010000393          323 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.406, whole genome shotgun sequence.
ACCESSION   DAXWOO010000393 DAXWOO010000000
VERSION     DAXWOO010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.406"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACGE70_003055"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACGE70_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFF8694580.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT          100 a           49 c           51 g          123 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttatatc aactcatttc ttg
//
LOCUS       DAXWOO010000394          303 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.408, whole genome shotgun sequence.
ACCESSION   DAXWOO010000394 DAXWOO010000000
VERSION     DAXWOO010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.408"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..259
                     /locus_tag="ACGE70_003056"
     CDS             <1..259
                     /locus_tag="ACGE70_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFF8694581.1"
                     /translation="KYSKACASLDDGFEDAFQYTVQGNSHNRLKSTNLIERLNQEVRR
                     REKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT          114 a           56 c           53 g           80 t
ORIGIN      
        1 aaaatattca aaagcttgcg catcattgga tgatggattc gaagacgcct ttcaatatac
       61 cgtacaagga aattcccaca atcgactaaa gagtaccaat ctaattgaac gactgaatca
      121 agaagtacgc agaagagaaa agattattcg catcttcccc aatcaaacat cagccaatcg
      181 cttaattgga gccgttctta tggacctaca tgatgaatgg atttattctt caagaaaata
      241 catcaatttt gataagtaga aatggtaaaa acattgtata gcattttaca caggagtctg
      301 gaa
//
LOCUS       DAXWOO010000395          301 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.409, whole genome shotgun sequence.
ACCESSION   DAXWOO010000395 DAXWOO010000000
VERSION     DAXWOO010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.409"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..>301
                     /locus_tag="ACGE70_003057"
     CDS             <1..>301
                     /locus_tag="ACGE70_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFF8694582.1"
                     /translation="NKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYASR
                     LDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYV"
BASE COUNT          103 a           34 c           56 g          108 t
ORIGIN      
        1 tgaacaaaca taatagaaaa ataacgaata acaaagaact gatcttgtta ctaataggga
       61 tttggggtat ctttttactc tttaatatgt atatgccgac tatgagagcg gatgacgtag
      121 tttatgcaag tagattagat gaacttggct atttgggtgc ttcgatagaa cattataaga
      181 catggagttc aagaattatt atagaactat tcttaatgtt cttttctaaa cactttatgc
      241 tatggaaatt attaaattct acgattatgt taggaattgt agtattactt tgcaagtatg
      301 t
//
LOCUS       DAXWOO010000396          300 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.410, whole genome shotgun sequence.
ACCESSION   DAXWOO010000396 DAXWOO010000000
VERSION     DAXWOO010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.410"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..>300
                     /locus_tag="ACGE70_003058"
     CDS             <1..>300
                     /locus_tag="ACGE70_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFF8694583.1"
                     /translation="AFQLVKQSKRLFPEINCVVADAGYKTPKFVHFLTHLNLRPCLPY
                     SRPKGKKELLSKNEFLYDEYFDCYICPQDQMLAFSTVTREGYREYKSNPKECVNC"
BASE COUNT           87 a           59 c           58 g           96 t
ORIGIN      
        1 tcgcctttca gctcgtcaaa caatcgaaga gactttttcc tgaaatcaat tgtgttgtcg
       61 ctgatgctgg gtataagaca cctaagtttg ttcacttttt gactcatttg aaccttcgac
      121 cttgtttgcc ttattcaagg cccaaaggga aaaaggagct attatcaaaa aatgagtttc
      181 tctatgacga gtattttgat tgttatattt gtccgcaaga tcaaatgctc gctttttcaa
      241 cggttactcg agaagggtac cgagaataca aatccaatcc aaaagaatgt gtgaactgtc
//
LOCUS       DAXWOO010000397          298 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.412, whole genome shotgun sequence.
ACCESSION   DAXWOO010000397 DAXWOO010000000
VERSION     DAXWOO010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.412"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            69..>298
                     /locus_tag="ACGE70_003059"
     CDS             69..>298
                     /locus_tag="ACGE70_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694584.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIE"
BASE COUNT          135 a           44 c           45 g           74 t
ORIGIN      
        1 cttcttgttt tcatataacc aaaggcgcta tgatttccat atgaaaaaaa taaagtcagg
       61 tgaaaaaaat gaaacttgag gataaaaaag tcattttgaa gaaaatcaaa cgactaccag
      121 accaaataga tggctatacg atctgttctt tcaaaaaaga caggagtata aaagcttata
      181 cgcaaaacac agaaaaagga ataatctatt tcctacatga agaaggttac gatacccaaa
      241 cctttatgtt tactgaaaaa aaagaatttc ataaacagat gaaaaaactg attgaaaa
//
LOCUS       DAXWOO010000398          291 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.413, whole genome shotgun sequence.
ACCESSION   DAXWOO010000398 DAXWOO010000000
VERSION     DAXWOO010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.413"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..272
                     /locus_tag="ACGE70_003060"
     CDS             <1..272
                     /locus_tag="ACGE70_003060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694585.1"
                     /translation="KGQRVSTPSRGIKNTPVLTGVWVTDRLDFNWCQKLMSRKGDEIL
                     AIYGYSRISTSNQDYKTQIQTLENAGAEIIFLENHWIIEHLWKYF"
BASE COUNT           98 a           50 c           63 g           80 t
ORIGIN      
        1 ttaaggggca gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg
       61 gtgtatgggt tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg
      121 gtgatgaaat tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca
      181 aaacacaaat tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact
      241 ggattatcga acacctttgg aagtattttt gagttacgta agtattgatg a
//
LOCUS       DAXWOO010000399          290 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.414, whole genome shotgun sequence.
ACCESSION   DAXWOO010000399 DAXWOO010000000
VERSION     DAXWOO010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.414"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..>290
                     /locus_tag="ACGE70_003061"
     CDS             <1..>290
                     /locus_tag="ACGE70_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFF8694586.1"
                     /translation="EDKATTYLLIDHSEKVAVNSSAKYHYYQSPKSILRGSFNPRIFD
                     SFEVHEDILVFIDKFYPELSINVRTRYVYEAIRMMMTMIKNNYTNKEDYLRS"
BASE COUNT          105 a           43 c           46 g           96 t
ORIGIN      
        1 tggaagataa ggctactact tatttgttga tcgatcattc cgaaaaagtt gctgtcaatt
       61 catctgctaa atatcactat taccaaagcc ctaaaagtat actgagagga agcttcaatc
      121 ctagaatttt tgattctttt gaagtccatg aagatatact agtattcata gataagtttt
      181 atccggaatt atcaattaat gttagaactc gctatgtcta tgaagcaata aggatgatga
      241 tgacaatgat taaaaataac tataccaata aagaagatta tttacgttct
//
LOCUS       DAXWOO010000400          280 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.415, whole genome shotgun sequence.
ACCESSION   DAXWOO010000400 DAXWOO010000000
VERSION     DAXWOO010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.415"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..>280
                     /locus_tag="ACGE70_003062"
     CDS             <1..>280
                     /locus_tag="ACGE70_003062"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8694587.1"
                     /translation="RYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIED
                     LPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIE"
BASE COUNT          119 a           27 c           47 g           87 t
ORIGIN      
        1 caagatatat taaattgatt tttgaatttt tgaaaaaaga aagaaatgct aatgcagtta
       61 ttttagaccc tcataaaaat aatccaagag caataagggc ataccaaaaa tctggtttta
      121 gaattattga agatttgcca gaacatgaat tacacgaggg caaaaaagaa gattgttatt
      181 taatggaata tagatatgat gataatgcca caaatgttaa ggcaatgaaa tatttaattg
      241 agcattactt tgataatttc aaagtagata gtattgaaat
//
LOCUS       DAXWOO010000401          274 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.416, whole genome shotgun sequence.
ACCESSION   DAXWOO010000401 DAXWOO010000000
VERSION     DAXWOO010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.416"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
BASE COUNT           83 a           42 c           31 g          118 t
ORIGIN      
        1 acttttgttt cgtgttaagt ttctttacat tcctacgcac cttgcgtgta aaatttcttt
       61 attcctccca cgctccatgt tattttatat ttcctttttc atgttatttt tatccagaaa
      121 ccgaacaagt ttttacctat tcccaaaatt aatttattag ttattttttc attgattaag
      181 aataattatt tgatgcttaa attagctaaa aaaacgattt tttactgata taatcatgat
      241 attaaagctg aattaggcgg aaagataaaa tgtt
//
LOCUS       DAXWOO010000402          261 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.417, whole genome shotgun sequence.
ACCESSION   DAXWOO010000402 DAXWOO010000000
VERSION     DAXWOO010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.417"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..>261
                     /locus_tag="ACGE70_003063"
     CDS             <1..>261
                     /locus_tag="ACGE70_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFF8694588.1"
                     /translation="FPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLKIHMIVTK
                     TGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHK"
BASE COUNT           95 a           47 c           43 g           76 t
ORIGIN      
        1 gttttcctag tccattatgt aaaccagtta gaaatagaca agcaaaacta ttgaatcaaa
       61 tagccaaagt tggctataac tcaacaaaaa aatcttattt ttatggtctt aaaattcata
      121 tgatcgttac taagactggc tttccgatta cttactcaat cacgaatcca ggtgtccatg
      181 acgtgaaagt gttagagact ttatcggaag aagcgaatct tcccaatatc ctaggggaca
      241 aaggatatat tagccataaa a
//
LOCUS       DAXWOO010000403          258 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.418, whole genome shotgun sequence.
ACCESSION   DAXWOO010000403 DAXWOO010000000
VERSION     DAXWOO010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.418"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            1..258
                     /locus_tag="ACGE70_003064"
                     /pseudo
     CDS             1..258
                     /locus_tag="ACGE70_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611996.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          105 a           41 c           51 g           61 t
ORIGIN      
        1 cattcgagaa tatgaactaa cggcttcagc atttgacaaa tgggtaaagc aatctaaaac
       61 gagtggttca ttcaaagaaa aagataatct tacgcctgaa caaaaagaat tgttagaact
      121 acgtaaaaga aaccagcaat tagaaatgga aaatgatatt ttaaagcaag cagcgctgat
      181 attcggacga agagacaagt aatcgatgcg aataagcatc tttaccctat atcagcgatg
      241 tgcagaatat taggtcta
//
LOCUS       DAXWOO010000404          256 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.denovo.419, whole genome shotgun sequence.
ACCESSION   DAXWOO010000404 DAXWOO010000000
VERSION     DAXWOO010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.denovo.419"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..245
                     /locus_tag="ACGE70_003065"
     CDS             <1..245
                     /locus_tag="ACGE70_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFF8694589.1"
                     /translation="IQAYNQSLRAVSKELNIDKKVSSHMLRHSHISLLTELGIPLKAI
                     MDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT          110 a           44 c           30 g           72 t
ORIGIN      
        1 ctattcaagc atataatcaa tctttaagag cagtatccaa agaattaaac atagataaaa
       61 aagtttcttc tcacatgtta aggcattccc acatttctct acttacagaa ttaggaatcc
      121 cattaaaagc cattatggat cgtgtaggac atgaagattc aaaaacaaca ttaaagattt
      181 atactcatac aactaaaaat atgcaaaatc aactagtaga aaaactagga aaaatagaaa
      241 tttaaattgc cccttt
//
LOCUS       DAXWOO010000405        10320 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.guided.100, whole genome shotgun sequence.
ACCESSION   DAXWOO010000405 DAXWOO010000000
VERSION     DAXWOO010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10320
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.guided.100"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            136..1032
                     /locus_tag="ACGE70_003066"
     CDS             136..1032
                     /locus_tag="ACGE70_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFF8694590.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1182..2123)
                     /locus_tag="ACGE70_003067"
     CDS             complement(1182..2123)
                     /locus_tag="ACGE70_003067"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFF8694591.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2249..3370
                     /locus_tag="ACGE70_003068"
     CDS             2249..3370
                     /locus_tag="ACGE70_003068"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFF8694592.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3628..5130
                     /gene="eat(A)"
                     /locus_tag="ACGE70_003069"
     CDS             3628..5130
                     /gene="eat(A)"
                     /locus_tag="ACGE70_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFF8694593.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5197..5724
                     /locus_tag="ACGE70_003070"
     CDS             5197..5724
                     /locus_tag="ACGE70_003070"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFF8694594.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            5935..6996
                     /locus_tag="ACGE70_003071"
     CDS             5935..6996
                     /locus_tag="ACGE70_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFF8694595.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            6999..7895
                     /gene="murQ"
                     /locus_tag="ACGE70_003072"
     CDS             6999..7895
                     /gene="murQ"
                     /locus_tag="ACGE70_003072"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFF8694596.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            7909..9891
                     /locus_tag="ACGE70_003073"
     CDS             7909..9891
                     /locus_tag="ACGE70_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFF8694597.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(9956..>10320)
                     /locus_tag="ACGE70_003074"
     CDS             complement(9956..>10320)
                     /locus_tag="ACGE70_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFF8694598.1"
                     /translation="EDPVNPEVTLKEIHENSDFTKETEPFFTFVPTYLDGGNGLDNGD
                     TEILTETMREYLEHEDNHKLCLGVVGSGNKNFNNQYCLTAKQYAQTFGFPFLADYELR
                     GTPSDVTRIYQILAENNK"
BASE COUNT         3379 a         1825 c         2237 g         2879 t
ORIGIN      
        1 gaagaatgat ttatttcatc atattttcat acaaatgtta aattttcata caaatatagg
       61 aaacgtttta ctggcttttt actatttaaa caggctatca taggaaaaag tgacagagaa
      121 agtgaggcgg aaaagatgaa caaaaatgac gtactgctag ttgcaaaaaa taaatcactt
      181 tattttttgc cttccatccg ctctttagat gtttggtccg gaaaagattt actacagtcg
      241 gaattcatta aatcagctag acaacagttg gcaaattatc agctgatcgt ttttttagat
      301 tatggatttg atatggagat ggctcgacgc gtgcgcccat ttaccaatgc aaaaattgtg
      361 ttattttttt ggaatcactt caaagcagaa cacgaggcga tgctgaagca ggcacaagat
      421 gaaccggcaa tcgatgagat ttatcatttt gattttttgg aagcaaaaga acttgggctg
      481 aaacataata gttcatttta ttcgaaacat atgaattttt cttctagcga accaaagacg
      541 gatctatttt ttggtgcaac gaataatggg agaaaagaac gagcagaaag ctataagcag
      601 gaatttataa aaagagatgt tacgaccaac tatttcattc ttccacatcg agggaatgaa
      661 caacctggat atcttactta tgaagagtac ctgaaaaaaa caggggaatc gcgcgggatt
      721 ctggaactgt tgcgggaggg tcagcaagga gtgactttgc ggacatttga atccatgtat
      781 tttcaaaaga aactagtgac ggataatcaa gcgattgctc attatcattt ttatcgtcct
      841 gaaaatattt ttttgcttcg agaacgtgat ttagatgaat taccggaatt tctgcataca
      901 ccatttaagc caatcctatc agagctattg gaattttatg atgcagaaaa ttgggcaaaa
      961 cgatttatca ctaacgattc cactatttat gagcaatacg aatatgacct ctcctctgtt
     1021 gaaaacaaat aaaaaaccta cattttgaag agattatgta ctgaaacaaa caagcacaat
     1081 tcttatggca tcatgagagt tgtgttttct ttttgatctt ttattgggct taaaaaaaga
     1141 gaaccagctg ctgattcagc tgattctcat cagagagcct tttattctgc gccaaggatt
     1201 ccttcttttg catggactaa ttgggtcaaa aaggcacaat atggtgtagc aatatcatat
     1261 ttttgtcctt ttcgccaaat ggcaccattg atgtagtcga tttcagtcaa acgatggttt
     1321 ttgatcaggt cttggtacat agatgggtaa tgcaacccga tgttgtcagg atcataagta
     1381 gaagcaatat gttggtacac ttcttcttga tctagtatga ttccttcttt tgctgcgata
     1441 gaagcaaatt cagatacgat tgttcgaacc atgctctctg ccgctttttg cgcgcctagt
     1501 tcagcaatat tacaatccaa aatcgtgcat aaaccattca atgttccatt tacacaagct
     1561 tttcgccaaa tggaataacg aacgttatgg ctatagatcg gatttaaatt tgcttcttga
     1621 aagacttcta caactttttt tgtaaatgct tcgccttctg gttccaagtt ctctagttcg
     1681 atttctccat cacctaacaa tgtgactttt ccgggaccag ctagaccagc tgtccacatc
     1741 gtgatcccaa ataagatatt tttctttggc acatattttt ccagtacatc ttcatgacct
     1801 aatccattta gtaaacacaa aacataagta ttgtcagtaa taatggattg gatcgaacaa
     1861 aacatatcat ctaattgatt ggcttttgtt aatgctacga tcaagtccac gtgttcgttg
     1921 ctttcgatga tttcttctgg tgaatagatt ggcagttttg cgaccacttc tttaccgtta
     1981 aagtcagcaa tcaacccatt tgtacgaatg gcttcaatgt gagcaggcca acgatcgatc
     2041 aagaggacct catttccgct ttgatgtagc atcaatccga ttcggctgcc cattgctccg
     2101 gccccagcta ttgcgatttt catgtttttt ctctccttta ttcaatgagt tcccttttag
     2161 tatatagaaa aatggaagaa aatagcattg ttttgtttga aaaaaatgaa aaaaagtgtc
     2221 ttacttaaga agaaagtgag ggatacagat ggatattttg gcggcaattg attcatttaa
     2281 aggaagtgct tcttcatttg aactaaatca ggcagttcta aatggctggg aagatcgtaa
     2341 aggaagaaaa atcaatgtgc cgattgcaga tggcggagaa aatactgcag aagcaatcta
     2401 ttacgcgctc aggggaacat ggcgtaccat tgatgggatg gatctcttat tccgtcagcg
     2461 aaaaatcagc tatttattga caacttggca aggaaaaaac accgcagtca tcgaatctgc
     2521 acaagcattg gggttagatc tacttgataa accaacggat caaactatcc gacttgcttc
     2581 cagttatgcc ttaggagaac ttattaaaga tgcactgctt caagaagtag aacaaattat
     2641 tgtcacttta ggaggaagcg ggtgtagcga tggtggtcta ggattactcc aatccttggg
     2701 tgcttcttta gaagggtaca cagaaggaaa tcctttatta tctactaaaa aacttcattt
     2761 tgatcgaaga aacgttcttg tttctaaagt aaaattgatt atcgctgcag atgttaccaa
     2821 tccatataca ggtcaacaag gagctgcttg gcaatttgga agacaaaaag gaggaataga
     2881 tacgacgcta gctttcttag ataaacaagc aggaaagatt gctcagcaaa taaaagattt
     2941 ctgggaggtg gatttagatg atattccagg aagtggagca gcaggcggta ttggtggagc
     3001 cctttttttg ttgggagcga agatggtttc tggtttcgat ttagtcagcc aaatcactgg
     3061 attggaaaaa aagatcgaac aagcagatgt catcattaca ggagaagggc ggatcgatca
     3121 gcaaacgatc cacggaaaag ttccatacgg tgtagcagtg cttgcaaaaa aatatcgaaa
     3181 accaataatc gctatttgcg gaagtagaga ccaagagcta gaaggacttt ctgattttct
     3241 cccaattgtc ttaagtattc atttgggtcc aataggttta gaagaagcaa tggaacacag
     3301 gcaaacatta gaaaaaacaa aaatcttagg acagacactt agcagatttt tcaccatcct
     3361 cccgaagtag ttgacaaaat cattttcttt atgctatcgt aatttcaaat ataggacaaa
     3421 gtgagggatc cgtgcattga aaaattaagt cgattgatga aagctgcatt cagactgtac
     3481 cttgtggaga caaggaagca gtgtgcagat tttcgggatt ggcttggttt tttcgatgta
     3541 tgggtctttt tgtgtgtatc caacccttgc atcctcttta gccagttctg aaaataggac
     3601 tggtcttttt ttacgaggtg atggaaaatg tctaaaatcg aaataaaaaa tctgacattc
     3661 ggctacgaca gccaaggcac attattattt gaacaagcaa atctaaattt tgacacacaa
     3721 tggaaactag gacttatcgg acgaaacggt cgaggaaaga caactttact gaatattcta
     3781 caaaacaaac taccttatca agggcaagta atccatcagc aagaatttgc ctatttcccg
     3841 caacagacaa aagataaaga acgtttaacc tattacgtgt taaatgatat tacggatttt
     3901 gagatatggg aaatcgaaag agagctccaa ttgatgcaaa cagatcctga aatcttatgg
     3961 agagaattca gcacactatc ggggggagag aagacaaaag tcctactggc acttttattt
     4021 gtggatgaca ctcatttccc gttaatcgat gaaccaacga atcatttgga tatctctggt
     4081 agaaaacaag tagcggctta tttgaaaaag aaaaaacaag gcttcatcgt ggtcagccat
     4141 gaccggggat ttatcgatga agtagtggac catgttttag caatcgaaaa aagtcaactg
     4201 gaactttatc aagggaattt ctctatctat gaagaacaga aaaaacttcg tgatgaattt
     4261 gaaatggctc aaaatgaaaa attgaaaaaa gaagtcagta ggctaaagaa aacagcagct
     4321 gaaaaagccg aatggtctcg ttcccgagaa ggagataaaa caaagaaaca agtcggattc
     4381 atcgatactg aatctagacg agtgaataaa ggagcagtgg gtgctgatgc tgcacggacg
     4441 atgaaacgat ccaaagcaat cgtgaatcgg atggagaccc agatcagcga gaaagaaaaa
     4501 ctattaaaag atatcgaata tatcgattct ttgacgatga atagccaagc gtctcaccat
     4561 aagcgacttt taagcgtaga agatcttcaa ttagggtatg aaaatctgtt attcgagcca
     4621 attcatttta caatcgagcc tcatcagcgg gtggcgattt caggtcctaa cggtgcagga
     4681 aagtcatcca ttatccatta tcttctgggg gcattcaacg gcaaggttat aggagaaaaa
     4741 taccagccaa aacatctgag cattagttat gcaagccaaa attatgaaga caatcgagga
     4801 acgttggcgg aatttgcaga gaaaaaccaa gtagactacc aagcattttt gaacaacctc
     4861 cgaaagcttg ggatggaaag agatgttttt cataacaaga tcgagcagat gagtatgggc
     4921 caacggaaaa aagtggaatt ggctaaatct ttatcacagc cagctgaact atatacatgg
     4981 gatgaaccat tgaattattt ggatgtcttc aatcaagaac aattagaaca actgatcttg
     5041 aacgtgaaac ctgccatgtt actagtggaa catgatcaaa ccttcctgga taaagtatct
     5101 actgagatta tttctcttga gagaatctaa cccttgtaat ctcaagaaca acaaggtatg
     5161 gttaagggga actttgccaa cgaaggagag aatcacatgc tttatgtaac gagacacgga
     5221 gaaacaactt ggaacgcaca aggattagtt tgcggacggg cagatgtgcc gctgactgaa
     5281 aaaggacaga tgcaggcaca gaaactagca gaaaaggtag tagatttgcc tgttccaatc
     5341 acgaaaatca tccattcccc tcttcaacga gcaagagata cagcacaagc agttgctgat
     5401 cgtctcagct tgccgctgac cgttgatgaa cgattggtag aaatggattt tggggattat
     5461 gatggactgc caagcaaaga tgagaacttt caaaaagcac gtttagcgtt cgctgtacga
     5521 ttcccaaatg gggagtcagt cttagatgtg tatgcacgga tcgttccttt attgaaagaa
     5581 tgcatcgaag acgaagaaaa tgtctatctg ttagtttgcc ataatgcctt gataagagtg
     5641 atcaatgctt attttcatcc gatgcctaat gaggggttct ttacattcat ggtagataat
     5701 acagaattgg tttcttatga gtaattatgt ttttgtgtta tttaaataaa atatttcaag
     5761 gcactgcttt tgcttagcag tgcctttttt aggttatgac aattcttttg ataatataaa
     5821 ctcaaccttt agaaatgaaa tttaattatt ttaataaaat tattgaaatt ttgtttcttt
     5881 agatatatac tctagtcata gaaatgaaga cggatacaga aaaagaggga taatatgttt
     5941 ggcttttcaa tatttatgaa cgattctttg tcagaagaga agaaacagta tatccgccaa
     6001 atggcatcca atggctttgt cggtattttc acttccatgc atataccaga agatgatgtt
     6061 tcggcatatc aaaagcgatt gatcgaacta ggaagcgaag cgaaaaagaa taaactagaa
     6121 ttgatggtcg atatttcagg agaggcattg gaacgagcgg gtttttcatt agaagatcta
     6181 acgcctttga aagcaattgg tgtaacaggt ttaagaatgg attatcatat ttccaatcag
     6241 cagattgccg actggtcaca tcaattgaaa atcagtttga atgctagcac gatcacgcct
     6301 aaagatatcg atgaattgaa agaagcagaa gctgatttct cacagattga agcttggcat
     6361 aattattatc cacgtcccga aacagggctg gacaaagaat ggtatcagaa aaaaaatcaa
     6421 tggctcaaaa ttcaaggtct ccttgtccaa ggttttgtac caggagatac agagttgaga
     6481 ggcccactat atcaaggatt gccaactttg gaagaacatc gagggatcca tcctcttgca
     6541 ggcgctctag atctattagc aagtaacacg gatattgttt acatcggaga tgcaggtctg
     6601 tcagaaaacg ttcaggagca atttgcttct ttccaaaaag aacagacagt cttgctccat
     6661 acggaacctg tagatgaaga attttacgaa tatatattag ggaagcatac gaatcggcaa
     6721 gacgatgcaa gagatgtcat acgaagcgta gatgcacgtt ttcgagagat tccgcctatc
     6781 ccagcaagaa atacagctac gcgtatgaaa gggtctatta ctttagataa cgaaaagtat
     6841 ttgcgctata tgggtgagat ccaattaacc aaatatgatt tgccagcgga tgaaaaagtg
     6901 aatgtagtag ctaaagttat caaagaagag ctaccattga tcaaccaaat caaagctggt
     6961 atgaactatc aatttataag aaaagaagga agataaaaat gagcaaaatc aatttagaga
     7021 acctaacaac ggaacgcaga aaccaagaaa cttttggatt agatgagatg agtatttctc
     7081 aagccgttca actaatgaac aaagaagatc agaaagtggc cgctgcggtt gaaaaagaac
     7141 tgacaagtat cgaaccagtg attgctaaca cgattgagtc ctttaaaaaa ggtggacgtc
     7201 tgatttatat gggagccgga acaagtggaa gactaggtgt gttagatgct gctgaatgcg
     7261 taccgacatt cggtgtagaa ccggaaatgg tcataggtct aattgctggt ggagaagaag
     7321 caatgactgt agctgtcgaa ggggccgaag attcttatga gctagggcaa gaagatctaa
     7381 tcaagctgaa tttgacaaaa aatgatatag ttattggtat cgcagctagc ggtcgtacac
     7441 catatgtgat cggtggatta gattacgcaa aatcgatcgg tgctgtcaca ggaacgattt
     7501 cttgcaataa acaagccgaa atcagtaaac acgcggattt cccaatcgaa gtcgatgcag
     7561 gtccagaatt tttgacaggc tctactcgat tgaagtcagg aacagcacaa aaacttatct
     7621 tgaatatgat ttctactatc agtatgattg gtataggtaa agtattcaat aacttgatgg
     7681 tcgatgtcaa accaacaaat gaaaaactaa tcgaacgctc aaaacgaatc atcatgcaag
     7741 cgacagaagc tgattacgaa acagctgaaa agtactttga agaagcagat caaaatgtta
     7801 agctggctat cgtcatgatt ttgacaaata gtgataaaga ggaagctcaa gcaaaattag
     7861 ctgaagcaaa cggatttgta aaagggacct tatgaggggg aaaagaccat ggcaaaaaac
     7921 gaactgactt tggaagaact ggcaaaacaa atttataaag gggcaggcgg catgggcaat
     7981 gtagaaagca ttgtccactg catgactcgt gtgcgtatga agatcatcaa tgatgatctt
     8041 gtaaatgtct ctgaattaaa agcgattcca ggagtgcttg gtgtagtaga agatgaacag
     8101 cttcaagtca tcatcggacc tgggaaagtg aacaaagtag cacagcaaat ggttgatttg
     8161 gcaggcgtga aattaggaga aaaactttct tctgtaagta cagctacagc aaattcatca
     8221 gcatctggaa aagaccgtgt aaacgaacgg gcacaagaaa tgaaagcggc acaaaaatcg
     8281 aaacaaaaac catctaaaat caaatcggta ttaaaagaca tttctaatat ctttgtgcca
     8341 atgattcctg catttgtcgg tactgggatc gtggcaggga ttgccgcagt tttagcaaac
     8401 ttagtcacag ccggaacact tgatgcagca acatggcaac aatatatcga tgtgatgaac
     8461 atcttgaaaa acgggatgtt tagctaccta gttatctata caggtatcaa tgctgcacaa
     8521 gtttttggtg caacaccaac tcttggtgga gttatcgggg ccgttgtgat gctgacaggg
     8581 atgaatccgg aagcaccact gaaaaactta tttactggtg aagctcttgc agctggacaa
     8641 ggtgggatcc taggtgtcat cttcgctgtt tggctattgt caatcgttga gaaaaaactg
     8701 cataaagtag tacctgatgc ggtagatatc atcgttacac caactctttc attgattgcc
     8761 atcggtctga ttgaaatttt cttcatcatg cctttagcag gtttcatttc tgatggcatg
     8821 gtcggtggga tcaactgggt acttggcgtt ggtggagcat ttgccggctt cgttctaggc
     8881 acattattct tgccgatggt tatgttcgga ctgcatcaaa ttttgactcc aatccatgtg
     8941 caaatgattg atgaaactgg tcgtacgcta ttgcttccaa ttcttgcgat ggctggtgca
     9001 ggacaagtgg gtgcagcatt ggcattatgg attcgctgca aaaaagataa agaattaaca
     9061 gaaatgatca aaggagcttt accagtcggg atcctaggaa tcggcgaacc attgatttac
     9121 ggagtaacat tacctctagg acgcccattt attactgctt gtatcggtgg aggtatcggc
     9181 ggggcagtga tcggaatgat cggaaatgtc ggggctatcg ctattggacc ttcaggagcc
     9241 gctttgatcc cattgatctc tgacggtaaa tggttaggct acatccttgg attattggca
     9301 gcttatgctg gtggcttcgt tgctacattc ttcttcggta ttcctaaaga ccgtttagaa
     9361 aaagaagaat tagcgactga aacagccaca gtaaatgaag tgcctgaaaa agtagtacct
     9421 caaacttcag ctgaaagtgt gactatcctc tcatcagttg ctgacggaac agttgtttct
     9481 ttagataaag caagtgatcc agtttttgca caaaagatga tgggtgaagg ctactttgta
     9541 gaaccagaaa acggacatat ctattctcca gttactgggg tagtcagctc tgtcttccca
     9601 acgaaacacg ctattgggat cacaacgcca aatggtttag aaatcttgct tcatatggga
     9661 atcaacacag ttgacttagg cggtacgcca tttgatgtga aagtaacaga agggcagcaa
     9721 gtgacacctg atacgcttgt agcagaagct gacttagaag cgatcaaagc agcaggcaaa
     9781 gaaacatcca tgatcgtctt ggtaaccaat atggatcgtg taaaaaactt tgtcttggaa
     9841 aaaacaggta aagcaaaagc aaaagcacct gtgatggatg tcaaagctta atatgataga
     9901 aacaatagaa acgaaagaag aaactgaaat ctatttttca gcttcttctt ttttattatt
     9961 tattgttttc tgctaaaatc tgataaattc gtgtcacatc agatggtgtt ccccgtaatt
    10021 cataatctgc caagaaagga aagccaaatg tttgtgcata ttgtttggct gttagacaat
    10081 actgattatt gaagttttta tttccgctac cgactacacc aagacaaagt ttatgattgt
    10141 cttcatgttc cagatattca cgcatcgttt cggtcaagat ttctgtatca ccgttatcta
    10201 aaccatttcc gccgtctaaa taagtaggaa cgaaagtaaa aaatggttct gtttctttcg
    10261 taaaatcact attttcatgg atttctttta aagtaacttc aggattgaca ggatcttctg
//
LOCUS       DAXWOO010000406         8696 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.guided.118, whole genome shotgun sequence.
ACCESSION   DAXWOO010000406 DAXWOO010000000
VERSION     DAXWOO010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.guided.118"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..426
                     /locus_tag="ACGE70_003075"
     CDS             <1..426
                     /locus_tag="ACGE70_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFF8694599.1"
                     /translation="RKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMF
                     VDLGGICLEDSVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGV
                     TIGENAIVAANATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            493..1230
                     /locus_tag="ACGE70_003076"
     CDS             493..1230
                     /locus_tag="ACGE70_003076"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFF8694600.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            1461..2729
                     /locus_tag="ACGE70_003077"
     CDS             1461..2729
                     /locus_tag="ACGE70_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFF8694601.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            3216..3680
                     /locus_tag="ACGE70_003078"
     CDS             3216..3680
                     /locus_tag="ACGE70_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFF8694602.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            3664..4293
                     /locus_tag="ACGE70_003079"
     CDS             3664..4293
                     /locus_tag="ACGE70_003079"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFF8694603.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            4647..6125
                     /gene="msr(C)"
                     /locus_tag="ACGE70_003080"
     CDS             4647..6125
                     /gene="msr(C)"
                     /locus_tag="ACGE70_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFF8694604.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            6172..6618
                     /locus_tag="ACGE70_003081"
     CDS             6172..6618
                     /locus_tag="ACGE70_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFF8694605.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(6817..7332)
                     /locus_tag="ACGE70_003082"
     CDS             complement(6817..7332)
                     /locus_tag="ACGE70_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFF8694606.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            7449..8650
                     /locus_tag="ACGE70_003083"
                     /pseudo
     CDS             7449..8650
                     /locus_tag="ACGE70_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         2781 a         1516 c         1817 g         2582 t
ORIGIN      
        1 cgcaaacggc tagaaaatat cattagtgac aaagtagatg atacggtgac tgttctgctg
       61 cctttttata cagatttcgg caaacatatt agtataggga aaaatgtctt tatcaatcgt
      121 caagtgatgt tcgttgactt aggtggcatc tgtttagaag attcagtatt gattggtcct
      181 cgtgtcaacc tcatcacggt gaaccatcta accgatccaa aagaacgaag aggcttgtca
      241 gtaaagccaa tccacatcaa gaaaaatgct tggatcggag caggagcaac tattctgcca
      301 ggcgtaacga ttggcgagaa tgccattgtt gcagcgaatg caactgttac gaaagatgtc
      361 ccagacaata ccatcgttgc aggaatccct gccaaaatcg tcaaacctgt cgaacggatt
      421 atttgaacag taagatgaaa taagagatag tgagtataag caagagattt ttcaaaacag
      481 gaggagaata gaatgaatga tttatcagaa aaagtgattg tcatcatggg cgcttcaagc
      541 ggaatcggtg aagcaacaac gaagctatta gcagaaaaag gggctaaact agttatagct
      601 gcacgccgag aagatcgcct aaaagcaata aaagaatctt taccagaagc tgagctatat
      661 attcaaacag cagatgtaag agattttgca caagtccaag cagttatcga tttagcgatg
      721 gaaaagttcg gtcgaatcga tgtattgtac aataatgcag gaatcatgcc aaccgctcca
      781 ttagttgaag gtcaccgtga tgaatggcaa aatatgcttg atatcaacat catgggtgtg
      841 ttaaatggaa tttccgccgt acttccaatc atggaaaaac aaaaatctgg gcatatcatt
      901 tcaactgatt ctgtggcggg acatgttgtt tatcctgatt ccgccgttta ttgcggaaca
      961 aaatttgctg tccgtgcaat catggaagga ttacgtcaag agcaacggca aaataacatc
     1021 aaatctacga tcatctctcc aggagctgtt caaactgaat tatatcaaac catcagcgac
     1081 aaaaaggctg ctcttgaact tcatgaagcg caaaaagaat ggggattgac ctccgaagat
     1141 atagcttctg ctgtagcatt tgcgatagaa acgccagacc ggatgtctgt cagtaacatg
     1201 attatccgtc cgacgacaca ggaagtataa tccataacgg ataatatcca aagagaatta
     1261 cttgttaaat agttattcaa acgaccatca atttaaggcg tgaatggaga agaatattct
     1321 tcatttacgt ctttttatgt agaaaaagca aatttatcaa aaagaaacaa atgttattta
     1381 aatatggaca ttgacttgct tttacttttt tagttacact gttatttagg aaaagtttca
     1441 taattcaaag gagaaaaaag atgaaagcaa agaatttatt atttagcgtt attcttttag
     1501 gaaatctagc agttgccaac acaacagtgc tagctgaaga atcgacagaa actgtcgcaa
     1561 ctcagacatc gagttcttca gaaacaggaa ttgaaaacga tacagagagt actttgcagt
     1621 cagctactgt ctcagaaaat acaatttcaa ccgaagaacc aacaactgaa acaacagaac
     1681 aaacacaaac cgaagacagt acatcagcag aagctcagaa caatccagag aatgcagaag
     1741 aaatcacgaa ccaattgaca gatgggcaaa cattgacaca tacccatcaa gcagatttgt
     1801 ctgaattcga aaactatact ggctatgctt tttatgctcg ctcatctgct aattctcaac
     1861 aagcgttcat tgatagtatt gcctcaactg cacaaagttt ggcaagtgcc aatgacttat
     1921 atgcatcagt catgattgct caagccatcg ttgaaagtgg ctgggggaac agtactttgg
     1981 cttctgcccc taattacaat ctatttggga ttaaaggaag ctataatggc caatctgtta
     2041 cgatgcctac ttcggaatat gtgaatgggc aatggattac ggtgaatgcc gcttttagaa
     2101 aatatccttc gtacaaagaa tcattgcaag ataacgtaac ggtattgaag acgacttctt
     2161 ttcagccagg cgtttactac tattccggtg catggaaaag caatacgaac tcttacaaag
     2221 atgctacggc ttggctaaca ggtcgttatg ccactgcgcc taattatggt tctacattga
     2281 acaatgtaat cgaaacctat aatctgacac agtatgatac agcaccaact tcaacttctc
     2341 cgatgtatcg tctgtataac cgtcatacag gagaacattt atatacattg aatgccggag
     2401 aaaaaaatta tctgccaact gtaggctggg aatatgaagg gattgcttgg caagcaccaa
     2461 acagcggaca acctgtctac cgtttataca atccttacag tggagaccat cattacacaa
     2521 tggcacaatc tgagatcaac tctttaacaa aaatcggttg gcgttatgaa ggtctaagtt
     2581 tttattctgg tggttccaag ccaatttatc gactatttaa tccaaatgaa aaaactggta
     2641 cccatcacta taccttgagt gctaaggaaa gagattggct aacaccgatg ggctggagat
     2701 atgaaggaat aggattttat ggttattaaa aagaggtagt gaaaaagctg ccctgcatca
     2761 agtagtaaga acagccaaac aaaaacagat tttcatgttt tttgtttggt tgaacttatt
     2821 accgcagatg caagtttttg aacaccgttt actcaaaaaa ggttgtgaca acatcttgtc
     2881 ataacttttt tattactgtt taagcatact gatccaattt ctaatattcg acgggattct
     2941 tgcaaaaggc gatttcggaa atttttggcg gtgtaaaatt ttctactgca gaagagctat
     3001 cttctatagt ttgataagaa tcaataatct tggttttagc gttgtgcttt ctttatcatt
     3061 ttttccgttc attctacaaa aaatttcttt tctctatact tttctattga gagcgttttt
     3121 tttattacac agctgattct ttgacttttt tcttttttta ttctattatt gacaagtcaa
     3181 tcgttgacga gtcaattatt agaaaggagg aaattatgtc tttagaagag attagtcacc
     3241 ttttatatca aatcaaatta gccgatcaaa aaatcgtcca gatctttgaa gaaaatatcg
     3301 gtataagtct tactagatac gagatcatga tgagactgaa agaagaaaat ccattgctac
     3361 aaagcagttt gcaagaaaag ttgcagattg atcaaggagc agttactcgt catctgaagg
     3421 tattggaagc aaatggctat gtttcgcgtc agagaaatcc tcagaacaac cgggaagtgt
     3481 atgtagcgct aacagaaaaa gcccagaaga aaatcgagca ttgtgaaatg gaccataaag
     3541 atacaggttc cgtgctatcc ccggctttca gcgatgtaga aatgaaacaa tttctaacac
     3601 ttttagataa attgaatacc caactttgca atataacaac agaaacgaaa gaggttaata
     3661 ataatggaac aaaaaaatga tttttcaaca attatgactg gtagaagatc cgttcgcgta
     3721 tatgatgaaa catacaaaat cccacacgaa gaaatgctgg aaatgatcaa agaagcgacg
     3781 actgcacctt cttctgtcaa tatgcaacca tggcgctttg tggtcgctga aagcgaggaa
     3841 gcaaaagaaa tcttgcgtcc tttgatccgt ttcaatacga gacaaaatga tacttcttct
     3901 gcaatggtca tgatttttgg cgatatgcaa tgttacgaat atggcgaaga aatctataac
     3961 caagcttacg aatcagggaa gatgccaaaa gaagtcagag atcaacaatt agctgcgatc
     4021 attccttatt acaagagttt ttcaagagaa gaaatgaacg atgtagtcaa agtcgattct
     4081 agcttagcgg ctatgcaatt catgttagtt gcgcgtgatc atggctatga aacaaatcca
     4141 attggtggat tcgaagcaga tcagttggca gaggcatttg gtttggataa agaccgttat
     4201 gttccagtta ttattttatc aataggaaaa gcggtagaag aaggttacga gtctgttcgt
     4261 ttagcaccag aaaagatcac tacgttcgaa taaaaggttt tcagttacta ttcagtagct
     4321 attgcttttt ttgaatcgtg tgtggtaacc taataaaaaa ggaggtcgga aaaatgactg
     4381 catcgatgaa attacgtttc gaacttttga ataacaacta aataaattca aaagacatgt
     4441 ataaacaaca atttcatgga tggagtatgt ccattttttc gaacgacagt agaataggtg
     4501 aaagctatga cagatttggt gatttagtca tagttatttc gttcagagta ggaaaagatg
     4561 tgatgcagtt tttttgcaaa cagatttttc tgctcttttt tagtatcgat aattgttgtt
     4621 ttacatgaaa taggagtgta aaacatatgg aaaatttagc agtaaatata acaaatctac
     4681 aagtcagctt tggcaaccag ctagaactat ctattgattc tcttcgtgtc tatcagcaag
     4741 atcgtatagg gatcatagga gaaaatggtg tcggtaaatc gactttgctc aaactaatag
     4801 ccggtgaact ttttcccgat catgggaaaa tccaaacaga gatcaccttc aactacctgc
     4861 ctcaattaac ctatctcgct gaagcaaagg acctaaattt ggaattagcc agtcatttcc
     4921 agttaagact ggaagaaact tcagagcgga aatggagcgg aggggaagaa cgaaagatcg
     4981 agttgatacg tcttctttct tcttatgaac aagggatgct tctagacgag ccaacaaccc
     5041 atctagatag aaaaagtatt gatcgactga ttgaagagct tcgttattat tatggtacgc
     5101 tggtttttgt tagtcatgat cgctattttc tagatgagct agcatcgaaa atctgggaag
     5161 taaaagacgg agaaatccga gagttttcgg ggaattatag tgcctatctc actcaaaagg
     5221 aattggagaa aaagactcag ctacgagaag cagagtcgat catgaaagag aaaaaacgat
     5281 tggaaaaatc gatccaagaa aagaaaaaac aagcggaaaa gttggaaaaa gtgtccagta
     5341 aaaagaaaaa gcaacaaatc agaccggatc ggttgtcttc ctctaaacaa aaagacagtg
     5401 tacaaaaagc catccaaaag aatgcgaaaa cattagagag aagactccaa aaaataggag
     5461 aaacaaccaa gccgcagcag atgaaacaaa tccgttttcc agtaccaaaa tctcttgaac
     5521 tccacagccg ctatcccatc atgggacaaa atgtccaatt ggaaagaagt gggagaacat
     5581 tactggtaaa tggtgatttt cagttttctt taggtaaaaa aatcgcgatt gtcggtgaaa
     5641 atggttcagg taagacaacc ttattagaac atatccgcaa acaaggagaa ggaatccttc
     5701 tctctccgaa agtaagcttt caagtatatc agcaaaaggg ttatcaaatg acatctgaag
     5761 aatccatcat tcgttttgtc atgagacaaa cagagttttc agaatcgctt gtccgtagtt
     5821 tgctgaatca cttagggttt gctcaggaaa ctttgacgaa accgttatgt acattaagtg
     5881 ggggagaagc gacccgtctg acgattgctt tgctttttac taagccaagt aatgtgttgc
     5941 tgttagatga accgactaat tttattgata tggcaacgat cgaagcttta gagaagctaa
     6001 tgcaaatata tccgggaacg attttgttta cttctcatga ttcttacttt gtcgagcgta
     6061 cggctgatga agtttatgaa ataaaagggc agaaaataaa aaaagtactt acgagaaatt
     6121 tttaaatgtg ctacgatgat tttcgtgaca aatttaaagg agtggtacag attgcctaca
     6181 aataaaaaag aaggaataat ttttacaaca atgatgtgtt ttttgatggt cttggggatg
     6241 agcgcgtata atttatggct gcatcaagat cttcatttat caagtttgtt gattggtcta
     6301 gtgcctggat tcgtggtggc tttcattttg gatgtcttca tcatcggtgt gctggcgaaa
     6361 aaaattgcct ttaaactacc agtaaatcca gaaagcaagg tccaaatgat cttaactatt
     6421 tcatgcttga tggttttagg aatggtgaca tgtatgtctt tattcggtgt gatcatggaa
     6481 caaggaatac ctgatcaatt attctctgct tatatgaatg cttggaaaat gaatgtgatt
     6541 gccgctttac cgttgcagtt gatcatcgtt ggcccgcttt caagaagtgt gctaaaagca
     6601 attcaaagta atgaatgatg gaacactgtt atttgaaaaa aggccttggc atgctttttg
     6661 tcatagtctc ttgaatgcag ttctagaaaa atagtgatcc gaacgatagg ttttggcctg
     6721 tcgttcggat cgctttttct tttccaactg tattgctctt ttattgaaga taatttgctc
     6781 ttcaatagat atttctagaa aggttctgtt tatcccttac tcaaaaaact gctgcatttg
     6841 ttctcttact tgctgacacc attggagttc ctgctgacaa agcataattc tgcgctggat
     6901 cacaacataa cgtccaaatt cttgtttctt gtctagcgag aggtcattca attttttttg
     6961 atttttttgc atgattttgt tcagctgttg ttctctagtg aatagcagtc cagtagctgt
     7021 tgttttatcg agcgtcgaaa aagcatagat ttttgctaga aattcatctt tttgcgtagg
     7081 agcgggagtt tcctctttga tccattcctc tacaagagca cgtccttcat cagtcaagtg
     7141 gtagatcttt ttttgagagt gctcgtcttt gtccaaaacg gtcacataac cctgctggtc
     7201 taacttggca agcgttgtat agatctgact gtgatgggct tcccaaaaaa ttgtgatcag
     7261 ctgcttcaat tcatacccag aacgtggttt ggataaaagc acgcataata atatataggc
     7321 taaagtattc ataaaaatcc tacctctcgt cttattttct ttattatatc acagtaaagt
     7381 ttccacgttt tacatgtgca tatttacatg taaaataaat catgtatgat gctaatagag
     7441 agaaagggat ggaaatattg gagaatcaaa tagatataca agaacaaaca aatgaagaaa
     7501 caaaggggaa aagttcagcg ctagttgtag cagctacctg tatgatcgct tttatgggag
     7561 tgggtctagt agacccaatc ttaaaaacga ttgctgcaca gttaaacgct acgccagctg
     7621 aaacgaccct gttatttact agttacatgt tagtcactgg tgtggtgatg ctatttacag
     7681 gtttcttatc tagtcggatc ggtttgaaaa aacattgatg acaggattgt ttatcatcgt
     7741 tgtattcgct ggtcttggcg gattatctgg gaatatcgga atgctgattg ggttacgtgc
     7801 cggctggggt gtcgggaatg ctttatttgt ctctactgct ttagcaacga tcgtcagtat
     7861 tttagttggt aacacagaaa aagcaatcat gatgtatgaa gcagcacttg gctgcggaat
     7921 ggctgttggc cctctagtcg gtggggtctt gggcagtggt tcatggcgtt atccattttt
     7981 tggggtagca tttttgatgt tttgcggttt tatagcccta gcggtacttt tacctgaaac
     8041 tgagaaacca aagagaaaag taggcctttg ggaaggtgtc aaagcgttaa gcaatcataa
     8101 acttcgttca gttggactaa tcgcattgtt atataatttc ggattcttta ctttacttgc
     8161 gtatgcgcca tttttattga ttggctattc tgaattagaa gtcggttttg tattttttgg
     8221 ctggggtgtg cttctggcta ttgcttcgat tttcgttgca ccggctcttg agcggaagtt
     8281 tacgacttat cggacaattt tagcagcatt tgtcttattc attatttgtt tgctgttatt
     8341 aggagctgga acttctatgc cagtccttgt accgatcagc attgtgttag caggattttt
     8401 ccaaggaatt atcaatacac tgttgacgac gattgctatg gaaataccag gtctgcaacg
     8461 aaacgttgct tcgtccagct atagttttgt ccgctttttc ggtggagctt tagcaccatt
     8521 tattgcaggt gtgattggtg aacatattgg tgaaagctat gcgttttatt ttgcaggtgc
     8581 cattgttctt atcagtgtat gttgtatcat ttatcaccgt aattattttg ctacaaatga
     8641 agaagaataa gaaatcagag gttgtgaaaa agctgtcctg catcaagtca taagaa
//
LOCUS       DAXWOO010000407         7477 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.guided.138, whole genome shotgun sequence.
ACCESSION   DAXWOO010000407 DAXWOO010000000
VERSION     DAXWOO010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7477
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.guided.138"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            108..191
                     /locus_tag="ACGE70_003084"
     CDS             108..191
                     /locus_tag="ACGE70_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFF8694607.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            316..1053
                     /gene="erm(B)"
                     /locus_tag="ACGE70_003085"
     CDS             316..1053
                     /gene="erm(B)"
                     /locus_tag="ACGE70_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFF8694608.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1223..1483
                     /locus_tag="ACGE70_003086"
                     /pseudo
     CDS             1223..1483
                     /locus_tag="ACGE70_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
     gene            1646..2311
                     /locus_tag="ACGE70_003087"
                     /pseudo
     CDS             1646..2311
                     /locus_tag="ACGE70_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558228.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2347..2592
                     /locus_tag="ACGE70_003088"
     CDS             2347..2592
                     /locus_tag="ACGE70_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFF8694609.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            2695..3564
                     /locus_tag="ACGE70_003089"
     CDS             2695..3564
                     /locus_tag="ACGE70_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFF8694610.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3545..4279
                     /locus_tag="ACGE70_003090"
     CDS             3545..4279
                     /locus_tag="ACGE70_003090"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFF8694611.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4312..5220
                     /locus_tag="ACGE70_003091"
     CDS             4312..5220
                     /locus_tag="ACGE70_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFF8694612.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            5217..5759
                     /gene="sat4"
                     /locus_tag="ACGE70_003092"
     CDS             5217..5759
                     /gene="sat4"
                     /locus_tag="ACGE70_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HFF8694613.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            5852..6646
                     /locus_tag="ACGE70_003093"
                     /note="aph(3')-IIIa"
     CDS             5852..6646
                     /locus_tag="ACGE70_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFF8694614.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            6922..7464
                     /locus_tag="ACGE70_003094"
     CDS             6922..7464
                     /locus_tag="ACGE70_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFF8694615.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIEPVLLQSFK"
BASE COUNT         2622 a         1129 c         1566 g         2160 t
ORIGIN      
        1 tatttgatag tagatttaaa actttgcaac agaaccattt tgtgtataat aggaattgaa
       61 gttaaattag atgctaaaaa tttgtaatta agaaggaggg attcgtcatg ttggtattcc
      121 aaatgcgtaa tgtagataaa acatctactg ttttgaaaca gactaaaaac agtgattacg
      181 cagataaata aatacgttag attaattcct accagtgact aatcttatga ctttttaaac
      241 agataactaa aattacaaac aaatcgttta acttctgtat ttatttacag atgtaataac
      301 ttcaggagtg attacatgaa caaaaatata aaatattctc aaaacttttt aacgagtgaa
      361 aaagtactca accaaataat aaaacaattg aatttaaaag aaaccgatac cgtttacgaa
      421 attggaacag gtaaagggca tttaacgacg aaactggcta aaataagtaa acaggtaacg
      481 tctattgaat tagacagtca tctattcaac ttatcgtcag aaaaattaaa actgaacatt
      541 cgtgtcactt taattcacca agatattcta cagtttcaat tccctaacaa acagaggtat
      601 aaaattgttg ggagtattcc ttaccattta agcacacaaa ttattaaaaa agtggttttt
      661 gaaagccatg cgtctgacat ctatctgatt gttgaagaag gattctacaa gcgtaccttg
      721 gatattcacc gaacactagg gttgctcttg cacactcaag tctcgattca gcaattgctt
      781 aagctgccag cggaatgctt tcatcctaaa ccaaaagtaa acagtgtctt aataaaactt
      841 acccgccata ccacagatgt tccagataaa tattggaagc tatatacgta ctttgtttca
      901 aaatgggtca atcgagaata tcgtcaactg tttactaaaa atcagtttca tcaagcaatg
      961 aaacacgcca aagtaaacaa tttaagtacc gttacttatg agcaagtatt gtctattttt
     1021 aatagttatc tattatttaa cgggaggaaa taattctatg agtcgctttt ttaaatttgg
     1081 aaagttacac gttactaaag ggaatggaga taaattatta gatatactac tgacagcttc
     1141 caagaagcta aagaggtccc tagcgcctac ggggaatttg tatcgataag gggtacaaat
     1201 tcccactaag cgctcgggac cccttgtagg aaaatgtcct aagtgtggca acaatattgt
     1261 attaaaaaaa tcgttttatg gttgttcaaa ttatcctgaa tgtaagttta ctttagctga
     1321 acattttaga aagaaaaaac taaccaaaac gaatgtaaaa gaattactgg agggaaaaga
     1381 aaccctggta aaaggaatca aaaacaaaga gaaaaagccc tacaatgccg ttgtaaaaat
     1441 tggggaaaag ggatatattg attttatctc tttttcaaaa taaacatggg gctgacccaa
     1501 aagtagctcc tatatgaaaa atagaggtct ttccatttag atagtagtgg aaagacctct
     1561 atttatcttt tgaaagtgaa tacaaaaata aagcacactc tgctataata taggtgatta
     1621 atccatttga gagaggtgct ttatcatgta tcaaaattat accataaacc aactttgttt
     1681 gcctatagat cttgaaatta aattagaaga aaatgatttt gctcatgcca ttgtgcagtt
     1741 tgttgactct attcccgatg aagtctttct tccttattat caatctatgg gtagacccca
     1801 atatcatcct cgtatgctac tttccattat tctttgtgcc tatattcaag gggtttattc
     1861 gggccgccaa atacaaaata tgctgataga tagtattcgg atgcgctact tatctcaaga
     1921 gcaattccct aactttcgta ctattaatcg ttttcgcgtt catccgatta tgaacgatat
     1981 tctagatcat tcatttgttc aatttcgtga gctactggtt caaagtggct taatcagtgg
     2041 cagtgctcta tacattgatg gtacaaaaat tgaagcggat gcgaataaat actctttcgt
     2101 ttggaaaaaa agtattttaa agtataagga tagccttgat caaaaagccc ttgaaaacta
     2161 ccaacaaatg gtggaaacaa aaatcttacc tgagttaata gatgatttaa aggatgaact
     2221 ttctatagaa gagattgaaa ggattcgtca atctttggaa gaaaaggaaa atcaattaat
     2281 tgaagcgatt gaccaaaccg aaacagtaga agggggctga cccattatta ataaggagtg
     2341 gacaacatga ttgatgtaaa tgagctattg gaagaagcta ttagagaaac tgaaaatcta
     2401 aatgatggcg aagtttttct tgttaaagat ttattcaagg gatatgtggg gaataggata
     2461 cccagaaagg atcgacttct gcttggaacg ttatttttaa accatgttag caaaatgaat
     2521 ggtaatttaa aggctattga aaagacttca tctaatcagc agaggtataa aaagacaatt
     2581 gataagtttt aactatcttt tgtgaaatta aaaaaattat aaatatgttg agtagaagca
     2641 aaagatttgg cttggaattt ttacagcagt gaatcgataa aaggagcatg cttgatggta
     2701 gataatatta ttaaatcagt agcagagaaa ttatcctctc tgtcttatat agaaggtatt
     2761 gttttaggtg gttcacgtgc aaggggcacc catacagagg attcggatat agatatcggc
     2821 atctattaca attcagaatc atttgacata aatactatta atcaattcgc tacaaagctg
     2881 gatgatgagc atagaaataa ccttgttgta cctcccggag catggggtga ttggattaat
     2941 ggcggcggat ggttagtcat aaacgggtat catgtggatt taattttacg tgatattaaa
     3001 cgtgtggaac aaataatgaa agatacagag cacggaattg ttactgccaa ttatcagact
     3061 gggcatcccc atggttatat tagtgcaatg tatcgaggag aattagcgat tagcaaaata
     3121 ctatatgcta agaatgaaag cttatgcgaa ttaaaaaaac aggcagaaac ttatcccaat
     3181 gctttgcaga aaagtttagt taactttttt atgtttgaag cagggttctc tttaatgttt
     3241 gtaaaagcaa attcgggaac agacgataaa tattatattg cgggtcatgt ttttcgtata
     3301 gtttcatgtt taaatcaagt gttatttgca tgtaataatg cttattgtat caacgaaaag
     3361 aaagctataa aactgcttga aacttttgaa cataaacctg aaaaatatac cgagaaggta
     3421 aatcatattt ttgaagtact cggtatctca ctttttgaat gctacgacat gaccgagaag
     3481 ctttataatg aagtgaatga aattgtatcg gagataaata actttttaaa cgaggagagt
     3541 tcagatgaaa gaaaacaaat atgatgataa tatatttttt caaaaataca gtcaaatgag
     3601 tcgctcgcag aaaggactgg ctggtgcggg agaatgggag actttgaaaa agatgctacc
     3661 tgattttaag ggtaagcgtg tgcttgattt aggatgcggc tatggatggc actgtatata
     3721 tgcgatggaa aacggtgctt cctctgtagt aggtgttgat atttctcata aaatgctcga
     3781 agtagcaaaa ggaaaaaccc attttccaca gattgaatat gaatgctgtg ccatagaaga
     3841 tgtggatttc ccagaggaga gctttgatgt aatactaagt tcgcttgcgt ttcattatgt
     3901 agcagactat gagaatttaa taaaaaagat atataggatg ctgaaggctg gtggcaattt
     3961 agtttttaca gttgaacatc ctgtttttac tgctcatgga acacaagact ggtattataa
     4021 cgaaaaagga gaaatactgc atttcccggt ggacaattat tattatgagg gcaaacggac
     4081 agctatgttt ttggaagaaa aggttacaaa atatcataga acactgacca catatctaaa
     4141 tacactgctt tcaaatagtt ttataataaa tcagattgtg gagccacagc cgccagagaa
     4201 catgatggat attccgggga tggcggatga aatgcgacgc ccaatgatgc tgattgtatc
     4261 ggcaaaaaag aagatgtaat aatatagaaa aaataaacga ggagtatgta aatgagatca
     4321 gaaaaagaaa tgatggattt agtactttct ttagcagaac aggatgaacg tattcgaatt
     4381 gtgacccttg aggggtcacg cgcaaatatt aatataccta aagatgaatt tcaggattat
     4441 gatattacat attttgtaag tgatatagaa ccgtttatat ctaatgatga ctggcttaat
     4501 caatttggga atataataat gatgcaaaag ccggaggata tggaattatt cccacctgaa
     4561 gaaaagggat tttcctatct tatgctattt gatgattaca ataaaattga tcttacctta
     4621 ttgcccttgg aagagttaga taattaccta aagggcgata aattaataaa ggttctaatt
     4681 gataaagatt gtagaattaa aagggacata gttccgactg atatagatta tcatgtaaga
     4741 aagccaagcg caagggagta tgatgattgc tgcaatgaat tttggaatgt aacaccttat
     4801 gttattaaag gattgtgccg taaggaaatt ttatttgcta ttgatcattt taatcagatt
     4861 gttcgccatg agctgctgag aatgatatca tggaaggtcg gcatcgaaac aggctttaaa
     4921 ttaagtgtag gcaagaacta taagtttatt gaaaggtata tatccgagga tttgtgggag
     4981 aaacttttgt ccacctaccg gatggattcc tatgaaaaca tatgggaagc attatttcta
     5041 tgccatcaat tgttcagggc ggtatccggt gaggtggcgg aaaggcttca ttatgcctat
     5101 ccggagtatg ataggaatat aacaaaatat accagggaca tgtataaaaa atacactggt
     5161 aaaaccggct gcctggatag cacatatgcc gctgatatag aagagaggcg ggaacagtga
     5221 ttacagaaat gaaagcaggg cacctgaaag atatcgataa acccagcgaa ccatttgagg
     5281 tgataggtaa gattataccg aggtatgaaa acgagaattg gacctttaca gaattactct
     5341 atgaagcgcc atatttaaaa agctaccaag acgaagagga tgaagaggat gaggaggcag
     5401 attgccttga atatattgac aatactgata agataatata tctttactac caagacgata
     5461 aatgcgtcgg aaaagttaaa ctgcgaaaaa attggaaccg gtacgcttat atagaagata
     5521 tcgccgtatg taaggatttc agggggcaag gcataggcag cgcgcttatc aatatatcta
     5581 tagaatgggc aaagcataaa aacttgcatg gactaatgct tgaaacccag gacaataacc
     5641 ttatagcttg taaattctat cataattgtg gtttcaaaat cggctccgtc gatactatgt
     5701 tatacgccaa ctttgaaaac aactttgaaa aagctgtttt ctggtattta aggttttaga
     5761 atgcaaggaa cagtgaattg gagttcgtct tgttataatt agcttcttgg ggtatcttta
     5821 aatactgtag aaaagaggaa ggaaataata aatggctaaa atgagaatat caccggaatt
     5881 gaaaaaactg atcgaaaaat accgctgcgt aaaagatacg gaaggaatgt ctcctgctaa
     5941 ggtatataag ctggtgggag aaaatgaaaa cctatattta aaaatgacgg acagccggta
     6001 taaagggacc acctatgatg tggaacggga aaaggacatg atgctatggc tggaaggaaa
     6061 gctgcctgtt ccaaaggtcc tgcactttga acggcatgat ggctggagca atctgctcat
     6121 gagtgaggcc gatggcgtcc tttgctcgga agagtatgaa gatgaacaaa gccctgaaaa
     6181 gattatcgag ctgtatgcgg agtgcatcag gctctttcac tccatcgaca tatcggattg
     6241 tccctatacg aatagcttag acagccgatt agccgaattg gattacttac tgaataacga
     6301 tctggccgat gtggattgcg aaaactggga agaagacact ccatttaaag atccgcgcga
     6361 gctgtatgat tttttaaaga cggaaaagcc cgaagaggaa cttgtctttt cccacggcga
     6421 cctgggagac agcaacatct ttgtgaaaga tggcaaagta agtggcttta ttgatcttgg
     6481 gagaagcggc agggcggaca agtggtatga cattgccttc tgcgtccggt cgatcaggga
     6541 ggatatcggg gaagaacagt atgtcgagct attttttgac ttactgggga tcaagcctga
     6601 ttgggagaaa ataaaatatt atattttact ggatgaattg ttttagtacc tagatttaga
     6661 tgtctaaaaa gctttaacta caagcttttt agacatctaa tcttttctga agtacatccg
     6721 caactgtcca tactctgatg ttttatatct tttctaaaag ttcgctagat agagttctat
     6781 attagagatg taaagagttt tggtgaagag gtttgtgagt atggctttta taaccagggt
     6841 gcaatgagaa gcactaacag cagctagctt aagaacgcat aggatatact ttttatggaa
     6901 gtcacagagg aggaataaaa tttgaacagg attaacagag taacctctat tcttattcag
     6961 ctgcaatcaa aaaaaataat tcctgccaaa gaaattgcac agcgatttaa tataagctta
     7021 aggacagttt acagagatat ccggacactt gaagaagccg gaataccaat tggatctgag
     7081 gccggaaaag gatattttct tgtagagggc ttcctacttc cgccggtaat gtttacagcg
     7141 gcggaagtgg gtgcattgat tacagcaggg aaatttttaa attgccatgg agatgaatca
     7201 tttataaagg attttgattc agctatgtat aaaatcaaat ctattttgaa gcatggtgaa
     7261 aaaaactatg cacaggagtt ggagaacagt attaacgtgt acagcacatc tggacagaaa
     7321 aatacattgg cggataacgt tattgcagca attcagactg caatctgcaa taaaagggta
     7381 atatcaattc aatatcctgc atcaggaggg caagaaccgg aaagcagaat gatagagccg
     7441 gttctgttgc aaagttttaa ataaagaata aaatccc
//
LOCUS       DAXWOO010000408         5437 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.guided.169, whole genome shotgun sequence.
ACCESSION   DAXWOO010000408 DAXWOO010000000
VERSION     DAXWOO010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.guided.169"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            241..1209
                     /locus_tag="ACGE70_003095"
     CDS             241..1209
                     /locus_tag="ACGE70_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFF8694616.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1202..2233
                     /gene="vanA"
                     /locus_tag="ACGE70_003096"
     CDS             1202..2233
                     /gene="vanA"
                     /locus_tag="ACGE70_003096"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFF8694617.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2239..2847
                     /locus_tag="ACGE70_003097"
     CDS             2239..2847
                     /locus_tag="ACGE70_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFF8694618.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            3275..4186
                     /gene="vanY"
                     /locus_tag="ACGE70_003098"
     CDS             3275..4186
                     /gene="vanY"
                     /locus_tag="ACGE70_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HFF8694619.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYELWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            4339..4824
                     /gene="vanZ-A"
                     /locus_tag="ACGE70_003099"
     CDS             4339..4824
                     /gene="vanZ-A"
                     /locus_tag="ACGE70_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFF8694620.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(5111..>5437)
                     /locus_tag="ACGE70_003100"
     CDS             complement(5111..>5437)
                     /locus_tag="ACGE70_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HFF8694621.1"
                     /translation="KKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIKI
                     FYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDIL
                     SAIRVF"
BASE COUNT         1731 a          936 c         1185 g         1585 t
ORIGIN      
        1 tggtgtcatc caccaacatc aaaaagtata gagccataat tttttaggaa aatctcaagg
       61 ttatctttac tttttcttag gaaattaaca atttaatatt aagaaacggc tcgttcttac
      121 acggtagact taataccgta agaacgagcc gttttcgttc ttcagagaaa gatttgacaa
      181 gattaccatt ggcatccccg ttttatttgg tgcctttcac agaaagggtt ggtcttaatt
      241 atgaataaca tcggcattac tgtttatgga tgtgagcagg atgaggcaga tgcattccat
      301 gctctttcgc ctcgctttgg cgttatggca acgataatta acgccaacgt gtcggaatcc
      361 aacgccaaat ccgcgccttt caatcaatgt atcagtgtgg gacataaatc agagatttcc
      421 gcctctattc ttcttgcgct gaagagagcc ggtgtgaaat atatttctac ccgaagcatc
      481 ggctgcaatc atatagatac aactgctgct aagagaatgg gcatcactgt cgacaatgtg
      541 gcgtactcgc cggatagcgt tgccgattat actatgatgc taattcttat ggcagtacgc
      601 aacgtaaaat cgattgtgcg ctctgtggaa aaacatgatt tcaggttgga cagcgaccgt
      661 ggcaaggtac tcagcgacat gacagttggt gtggtgggaa cgggccagat aggcaaagcg
      721 gttattgagc ggctgcgagg atttggatgt aaagtgttgg cttatagtcg cagccgaagt
      781 atagaggtaa actatgtacc gtttgatgag ttgctgcaaa atagcgatat cgttacgctt
      841 catgtgccgc tcaatacgga tacgcactat attatcagcc acgaacaaat acagagaatg
      901 aagcaaggag catttcttat caatactggg cgcggtccac ttgtagatac ctatgagttg
      961 gttaaagcat tagaaaacgg gaaactgggc ggtgccgcat tggatgtatt ggaaggagag
     1021 gaagagtttt tctactctga ttgcacccaa aaaccaattg ataatcaatt tttacttaaa
     1081 cttcaaagaa tgcctaacgt gataatcaca ccgcatacgg cctattatac cgagcaagcg
     1141 ttgcgtgata ccgttgaaaa aaccattaaa aactgtttgg attttgaaag gagacaggag
     1201 catgaataga ataaaagttg caatactgtt tgggggttgc tcagaggagc atgacgtatc
     1261 ggtaaaatct gcaatagaga tagccgctaa cattaataaa gaaaaatacg agccgttata
     1321 cattggaatt acgaaatctg gtgtatggaa aatgtgcgaa aaaccttgcg cggaatggga
     1381 aaacgacaat tgctattcag ctgtactctc gccggataaa aaaatgcacg gattacttgt
     1441 taaaaagaac catgaatatg aaatcaacca tgttgatgta gcattttcag ctttgcatgg
     1501 caagtcaggt gaagatggat ccatacaagg tctgtttgaa ttgtccggta tcccttttgt
     1561 aggctgcgat attcaaagct cagcaatttg tatggacaaa tcgttgacat acatcgttgc
     1621 gaaaaatgct gggatagcta ctcccgcctt ttgggttatt aataaagatg ataggccggt
     1681 ggcagctacg tttacctatc ctgtttttgt taagccggcg cgttcaggct catccttcgg
     1741 tgtgaaaaaa gtcaatagcg cggacgaatt ggactacgca attgaatcgg caagacaata
     1801 tgacagcaaa atcttaattg agcaggctgt ttcgggctgt gaggtcggtt gtgcggtatt
     1861 gggaaacagt gccgcgttag ctgttggcga ggtggaccaa atcaggctgc agtacggaat
     1921 ctttcgtatt catcaggaag tcgagccgga aaaaggctct gaaaacgcag ttataaccgt
     1981 tcccgcagac ctttcagcag aggagcgagg acggatacag gaaacggcaa aaaaaatata
     2041 taaagcgctc ggctgtagag gtctagcccg tgtggatatg tttttacaag ataacggccg
     2101 cattgtactg aacgaagtca atactctgcc cggtttcacg tcatacagtc gttatccccg
     2161 tatgatggcc gctgcaggta ttgcacttcc cgaactgatt gaccgcttga tcgtattagc
     2221 gttaaagggg tgataagcat ggaaatagga tttacttttt tagatgaaat agtacacggt
     2281 gttcgttggg acgctaaata tgccacttgg gataatttca ccggaaaacc ggttgacggt
     2341 tatgaagtaa atcgcattgt agggacatac gagttggctg aatcgctttt gaaggcaaaa
     2401 gaactggctg ctacccaagg gtacggattg cttctatggg acggttaccg tcctaatcgt
     2461 gctgtaaact gttttatgca atgggctgca cagccggaaa ataacctgac aaaggaaagt
     2521 tattatccca atattgaccg aactgagatg atttcaaaag gatacgtggc ttcaaaatca
     2581 agccatagcc gcggcagtgc cattgatctt acgctttatc gattagacac gggtgagctt
     2641 gtaccaatgg ggagccgatt tgattttatg gatgaacgct ctcatcatgc ggcaaatgga
     2701 atatcatgca atgaagcgca aaatcgcaga cgtttgcgct ccatcatgga aaacagtggg
     2761 tttgaagcat atagcctcga atggtggcac tatgtattaa gagacgaacc ataccccaat
     2821 agctattttg atttccccgt taaataaact tttaaccgtt gcacggacaa actatataag
     2881 ctaactcttt cggcaggaaa cccgacgtat gtaactggtt cttagggaat ttatatatag
     2941 tagatagtat tgaagatgta aggcagagcg atattgcggt cattatctgc gtgcgctgcg
     3001 gcaagatagc ctgataataa gactgatcgc atagaggggt ggtatttcac accgcccatt
     3061 gtcaacaggc agttcagcct cgttaaattc agcatgggta tcacttatga aaattcatct
     3121 acattggtga taatagtaaa tccagtaggg cgaaataatt gactgtaatt tacggggcaa
     3181 aacggcacaa tctcaaacga gattgtgccg tttaagggga agattctaga aatatttcat
     3241 acttccaact atatagttaa ggaggagact gaaaatgaag aagttgtttt ttttattgtt
     3301 attgttattc ttaatatact taggttatga ctacgttaat gaagcactgt tttctcagga
     3361 aaaagtcgaa tttcaaaatt atgatcaaaa tcccaaagaa catttagaaa atagtgggac
     3421 ttctgaaaat acccaagaga aaacaattac agaagaacag gtttatcaag gaaatctgct
     3481 attaatcaat agtaaatatc ctgttcgcca agaaagtgtg aagtcagata tcgtgaattt
     3541 atctaaacat gacgaattaa taaatggata cgggttgctt gatagtaata tttatatgtc
     3601 aaaagaaata gcacaaaaat tttcagagat ggtcaatgat gctgtaaagg gtggcgttag
     3661 tcattttatt attaatagtg gctatcgaga ctttgatgag caaagtgtgc tttaccaaga
     3721 aatgggggct gagtatgcct taccagcagg ttatagtgag cataattcag gtttatcact
     3781 agatgtagga tcaagcttga cgaaaatgga acgagcccct gaaggaaagt ggatagaaga
     3841 aaatgcttgg aaatacgggt tcattttacg ttatccagag gacaaaacag agttaacagg
     3901 aattcaatat gaactatggc atattcgcta tgttggttta ccacatagtg cgattatgaa
     3961 agaaaagaat ttcgttctcg aggaatatat ggattaccta aaagaagaaa aaaccatttc
     4021 tgttagtgta aatggggaaa aatatgagat cttttattat cctgttacta aaaataccac
     4081 cattcatgtg ccgactaatc ttcgttatga gatatcagga aacaatatag acggtgtaat
     4141 tgtgacagtg tttcccggat caacacatac taattcaagg aggtaaggat ggcggaatga
     4201 aaccaacgaa attaatgaac agcattattg tactagcact tttggggtaa cgttagcttt
     4261 ttaatttaaa acccacgtta actaggacat tgctatacta atgatacaac ttaaacaaaa
     4321 gaattagagg aaattatatt gggaaaaata ttatctagag gattgctagc tttatattta
     4381 gtgacactaa tctggttagt gttattcaaa ttacaataca atattttatc agtatttaat
     4441 tatcatcaaa gaagtcttaa cttgactcca tttactgcta ctgggaattt cagagagatg
     4501 atagataatg ttataatctt tattccattt ggcttgcttt tgaatgtcaa ttttaaagaa
     4561 atcggatttt tacctaagtt tgcttttgta ctggttttaa gtcttacttt tgaaataatt
     4621 caatttatct tcgctattgg agcgacagac ataacagatg taattacaaa tactgttgga
     4681 ggctttcttg gactgaaatt atatggttta agcaataagc atatgaatca aaaaaaatta
     4741 gacagagtta ttatttttgt aggtatactt ttgctcgtat tattgctcgt ttaccgtacc
     4801 catttaagaa taaattacgt gtaagatgtc taaatcaagc aatctgatct ttcatacaca
     4861 taaagatatt gaatgaattg gattagatgg aaaacgggat gtggggaaac tcgcccgtag
     4921 gtgtgaagtg aggggaaaac cggtgataaa gtaaaaagct tacctaacac tatagtaaca
     4981 aagaaagccc aattatcaat tttagtgctg aggaattggt ctctttaata aatttcctta
     5041 acgttgtaaa tccgcatttt cctgacggta cccccaaact aagatttcat agatagattt
     5101 tctgataact ttaaaaaaca cgaatagcgg aaaggatgtc ttcattcgct aaagagtaat
     5161 aaactacttt tccatcttga cgtgattttg ccattccatg tttttttaaa aaacgtaaat
     5221 ggtgtgacgt tgtagcgaca ctagcagtaa gtatttctgc taaatcacag acgcacattt
     5281 ccttataggt ttctaaagca tagaaaattt taattctaga aaaatcgctg aaacatttcc
     5341 ctaacacaag taagtttgaa aaatcttttt gatccaattt attttttatt ccatttactt
     5401 tttctttatg tacgacagtc acttggcaaa ctttttt
//
LOCUS       DAXWOO010000409         3009 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.guided.224, whole genome shotgun sequence.
ACCESSION   DAXWOO010000409 DAXWOO010000000
VERSION     DAXWOO010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.guided.224"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            126..623
                     /gene="dfrG"
                     /locus_tag="ACGE70_003101"
     CDS             126..623
                     /gene="dfrG"
                     /locus_tag="ACGE70_003101"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFF8694622.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(695..2647)
                     /locus_tag="ACGE70_003102"
     CDS             complement(695..2647)
                     /locus_tag="ACGE70_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFF8694623.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2654..2890)
                     /locus_tag="ACGE70_003103"
     CDS             complement(2654..2890)
                     /locus_tag="ACGE70_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694624.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
BASE COUNT          910 a          532 c          506 g         1061 t
ORIGIN      
        1 gtattatgat aatataactt atagaaaaaa ttgcttattc aactaacggg caggataatg
       61 agaaaaggtt ttttgataga aataagaaat tagatggtaa attagttaaa aaggctgtgt
      121 tataaatgaa agtttctttg attgctgcga tggataagaa tagagtgatt ggcaaagaga
      181 atgacattcc ttggaggatt cccaaggact gggaatatgt taaaaatact acaaagggac
      241 atccgataat attaggtagg aagaaccttg aatcaatcgg aagagcctta cctgacagaa
      301 gaaatattat tctgacgaga gataaggggt ttacctttaa tggttgtgaa attgttcatt
      361 caatagaaga tgtttttgag ttatgtaaaa acgaagaaga aatttttatt ttcggaggag
      421 aacagattta taatttgttt ttcccttatg ttgagaaaat gtacatcaca aaaatacatc
      481 atgaattcga aggagatact ttttttccag aagtgaatta tgaggaatgg aatgaggtat
      541 ttgcccaaaa agggataaag aatgataaaa atccgtataa ctactatttt catgtatatg
      601 aaagaaaaaa cttattgagt taacgggtgc gttaattgaa gaagaacagt aaagataaag
      661 cacaagagct tgggattact ccaagctctt tttgtcattt gaaataaatt atatctttcc
      721 aatcaaattg tttatctgta atccctaatc tttgagcagg tgtcaaaacc tttttatccg
      781 ctgatttaaa agggcgacaa aagttgtagt aagttcttag aattgtaatc gcaaattgag
      841 catacttcgg attgaagtta gcgtaaatat agctcttctt atcaccacga gctgttgtta
      901 atggtctttc aagagatgat atataacgtc ttatctgttg aataaagcta ttggtcgaat
      961 ggtcattaac atttaatagc atctttgcga tttcgtttgg ttccagagca gataaatccg
     1021 ttctacaatc aactgaataa aatcctttat ccttagtggc taatggatgt ttgatagggt
     1081 tatcggcata tttgcgatat ctttctccat ctttactaat agcctcttcg tggaattgat
     1141 gacttgttag taattcagtt agataatgag atgccaaagt ccgtagaaac tttgttttga
     1201 atcctctgat atctccccaa tcgagcagtt ctgcttttgc ttgtccgaat tcttttaagc
     1261 attgttttct gcttttcgtc ttgttaactt ggcaaataaa atgatgtgca tcgctcaacc
     1321 ttaattcttt cgtgaatact ctataaaagg cgtttctaat agagaagtca tcatcactaa
     1381 tcattcgcca ttcgtcggca ttaaccattt gtttaattaa ccaatagtga gcagttgtag
     1441 tataaggagc atcaacattt aaaccatcga caaatctact cctattagta attttcccta
     1501 attcgtgaag ataagcattc ctattttcgc tatcgttagc ggatggttct tgtggataat
     1561 aagaccaatc taacctatca tttttacgac taaaagtatt gagatggtct tctttgaatt
     1621 ttcttgtatc ttcatttaat tcatccattg aaatattcca atcataggca acatcagaac
     1681 ggaacacata tcttgataag acctcagcag atacaactat atacgtttgc atattaaggt
     1741 cttcaaaccc cgtatatttc tttgaacctt gtcctttctt acggacgtta tttaggtagt
     1801 agtgcatctt gtccgtatta agccacatct cgttaaattt ctttgtttga aggggttgtg
     1861 tctcatatcg ttctaaaaac tccaaacaac gcctatacag ccactccagc ttatgatagt
     1921 aggttccaac tccaattcct aaaatatcac aagtacgact gactgaaacc cttccaacaa
     1981 ccatcttagc gaacattgga agaatggtgt tcttttgttg gtgataagta gtggtttctt
     2041 ctctttttgg tagaacatta gtgaacttct tacacgcctt acattgatag cgttgagatt
     2101 tgcccctact tttccccctt ttgtaaaatt gcttcggctc attgaatggg gttgattctt
     2161 cttccgagca gccttcttta tgaaattgat aatctggctc aacatcttgg atgctattga
     2221 tttctataag gcgtttaatt tcttcaacaa cagaccaatt agataaaggg gttactgtac
     2281 agttttgcga tacacctctg ccaataggat tgtcattgca gtacatacct ttatgacctt
     2341 tatctttgga actaccagtc attttatatc gacttggttt cccttttaca tctttgaatt
     2401 tgtgctgttc tttcccaaaa ttacaacaaa acggattgat gcagtaatta tattgaatgg
     2461 gatattcaga accgttccat ttaaaagacg ttggcaagaa caacattttg ttataccttg
     2521 ttgcaaatcg ttttggagtt aataaatcat attgtttagc cctatctttt aattcttcgt
     2581 ttgaaatcgg aattttaaca tctacaattt catcttcctt cgtggctaat cttttaagct
     2641 tggtcattgt ctatcaaccc actctatctt tgatacccat tttttcaaca atccgcttat
     2701 tacttcgctt gttggcaatc cattcaataa acttcttatc gtctaaaatg ttcaataaga
     2761 attcatccac tgcttcgctt tcagtacgct ctgcatattc agcgtattgc ttaactatca
     2821 cccttacttg ctctgatatt tcccaatcca cagacttagc gttcttattt tttggctcaa
     2881 gaaattgcat aggaaaatgc accccttttt gcttagtaaa ttgtaaccat tttaacacac
     2941 caatttgcat tcctacaata attatttcac gttcgcattt ttatatacat tttaacttac
     3001 attttgtgg
//
LOCUS       DAXWOO010000410         2555 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.guided.235, whole genome shotgun sequence.
ACCESSION   DAXWOO010000410 DAXWOO010000000
VERSION     DAXWOO010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.guided.235"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            <1..459
                     /locus_tag="ACGE70_003104"
     CDS             <1..459
                     /locus_tag="ACGE70_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFF8694625.1"
                     /translation="TLFDSRFKTLQQNLQEDDIIYVTDLTRITRSTQDLFELIDNIRD
                     KKASLKSLKDTWLDLSEDNPYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFK
                     GRLKKYHKNHAGMNYAVKLYKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            673..1368
                     /gene="vanR-A"
                     /locus_tag="ACGE70_003105"
     CDS             673..1368
                     /gene="vanR-A"
                     /locus_tag="ACGE70_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFF8694626.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1346..2500
                     /locus_tag="ACGE70_003106"
     CDS             1346..2500
                     /locus_tag="ACGE70_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFF8694627.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          881 a          454 c          535 g          685 t
ORIGIN      
        1 accttatttg atagtagatt taaaactttg caacagaacc tacaggaaga tgacatcatt
       61 tatgttacag acttaactcg aatcactcgt agtacacaag atctatttga attaatcgat
      121 aacatacgag ataaaaaggc aagtttaaaa tcactaaaag atacatggct tgatttatca
      181 gaagataatc catacagcca attcttaatt actgtaatgg ctggtgttaa ccaattagag
      241 cgagatctta ttcggatgag acaacgtgaa gggattgaat tggctaagaa agaaggaaag
      301 tttaaaggtc gattaaagaa gtatcataaa aatcacgcag gaatgaatta tgcggtaaag
      361 ctatataaag aaggaaatat gactgtaaat caaatttgtg aaattactaa tgtatctagg
      421 gcttcattat acaggaaatt atcagaagtg aataattagc cattctgtat tccgctaatg
      481 ggcaatattt ttaaagaaga aaaggaaact ataaaatatt aacagcctcc tagcgatgcc
      541 gaaaagccct ttgataaaaa aagaatcatc atcttaagaa attcttagtc atttattatg
      601 taaatgctta taaattcggc cctataatct gataaattat taagggcaaa cttatgtgaa
      661 agggtgataa ctatgagcga taaaatactt attgtggatg atgaacatga aattgccgat
      721 ttggttgaat tatacttaaa aaacgagaat tatacggttt tcaaatacta taccgccaaa
      781 gaagcattgg aatgtataga caagtctgag attgaccttg ccatattgga catcatgctt
      841 cccggcacaa gcggccttac tatctgtcaa aaaataaggg acaagcacac ctatccgatt
      901 atcatgctga ccgggaaaga tacagaggta gataaaatta cagggttaac aatcggcgcg
      961 gatgattata taacgaagcc ctttcgccca ctggagttaa ttgctcgggt aaaggcccag
     1021 ttgcgccgat acaaaaaatt cagtggagta aaggagcaga acgaaaatgt tatcgtccac
     1081 tccggccttg tcattaatgt taacacccat gagtgttatc tgaacgagaa gcagttatcc
     1141 cttactccca ccgagttttc aatactgcga atcctctgtg aaaacaaggg gaatgtggtt
     1201 agctccgagc tgctatttca tgagatatgg ggcgacgaat atttcagcaa gagcaacaac
     1261 accatcaccg tgcatatccg gcatttgcgc gaaaaaatga acgacaccat tgataatccg
     1321 aaatatataa aaacggtatg gggggttggt tataaaattg aaaaataaaa aaaacgacta
     1381 ttccaaacta gaacgaaaac tttacatgta tatcgttgca attgttgtgg tagcaattgt
     1441 attcgtgttg tatattcgtt caatgatccg agggaaactt ggggattgga tcttaagtat
     1501 tttggaaaac aaatatgact taaatcacct ggacgcgatg aaattatatc aatattccat
     1561 acggaacaat atagatatct ttatttatgt ggcgattgtc attagtattc ttattctatg
     1621 tcgcgtcatg ctttcaaaat tcgcaaaata ctttgacgag ataaataccg gcattgatgt
     1681 acttattcag aacgaagata aacaaattga gctttctgcg gaaatggatg ttatggaaca
     1741 aaagctcaac acattaaaac ggactctgga aaagcgagag caggatgcaa agctggccga
     1801 acaaagaaaa aatgacgttg ttatgtactt ggcgcacgat attaaaacgc cccttacatc
     1861 cattatcggt tatttgagcc tgcttgacga ggctccagac atgccggtag atcaaaaggc
     1921 aaagtatgtg catatcacgt tggacaaagc gtatcgactc gaacagctaa tcgacgagtt
     1981 ttttgagatt acacggtata acctacaaac gataacgcta acaaaaacgc acatagacct
     2041 atactatatg ctggtgcaga tgaccgatga attttatcct cagctttccg cacatggaaa
     2101 acaggcggtt attcacgccc ccgaggatct gaccgtgtcc ggcgaccctg ataaactcgc
     2161 gagagtcttt aacaacattt tgaaaaacgc cgctgcatac agtgaggata acagcatcat
     2221 tgacattacc gcgggcctct ccggggatgt ggtgtcaatc gaattcaaga acactggaag
     2281 catcccaaaa gataagctag ctgccatatt tgaaaagttc tataggctgg acaatgctcg
     2341 ttcttccgat acgggtggcg cgggacttgg attggcgatt gcaaaagaaa ttattgttca
     2401 gcatggaggg cagatttacg cggaaagcaa tgataactat acgacgttta gggtagagct
     2461 tccagcgatg ccagacttgg ttgataaaag gaggtcctaa gagatgtata taattttggc
     2521 tctttgtcaa atgatgttgg taaagagaaa tctta
//
LOCUS       DAXWOO010000411         2251 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.guided.253, whole genome shotgun sequence.
ACCESSION   DAXWOO010000411 DAXWOO010000000
VERSION     DAXWOO010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.guided.253"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            221..268
                     /locus_tag="ACGE70_003107"
                     /pseudo
     CDS             221..268
                     /locus_tag="ACGE70_003107"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            284..2203
                     /gene="tet(M)"
                     /locus_tag="ACGE70_003108"
     CDS             284..2203
                     /gene="tet(M)"
                     /locus_tag="ACGE70_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFF8694628.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
BASE COUNT          759 a          346 c          465 g          681 t
ORIGIN      
        1 attatttaag tatctattta aaaggagtta ataaatatgc ggcaaggtat tcttaaataa
       61 actgtcaatt tgatagtggg aacaaataat tggatgtcct tttttaggag ggcttagttt
      121 tttgcaccca gtttaagaat acctttatca tgtgattcta aagtatccgg agaatatctg
      181 tatgctttgt atgcctatgg ttatgcataa aaatcccagt gataagagta tttatcactg
      241 ggatttttat gcccttttgg gcttttgaat ggaggaaaat cacatgaaaa ttattaatat
      301 tggagtttta gctcatgttg atgcgggaaa aactacctta acagaaagct tattatataa
      361 cagtggagcg attacagaat taggaagcgt ggacaaaggt acaacgagga cggataatac
      421 gcttttagaa cgtcagagag gaattacaat tcagacagga ataacctctt ttcagtggga
      481 aaatacgaag gtgaacatca tagacacgcc aggacatatg gatttcttag cagaagtata
      541 tcgttcatta tcagttttag atggggcaat tctactgatt tctgcaaaag atggcgtaca
      601 agcacaaact cgtatattat ttcatgcact taggaaaatg gggattccca caatcttttt
      661 tatcaataag attgaccaaa atggaattga tttatcaacg gtttatcagg atattaaaga
      721 gaaactttct gccgaaattg taatcaaaca gaaggtagaa ctgtatccta atatgtgtgt
      781 gacgaacttt accgaatctg aacaatggga tacggtaata gagggaaacg atgacctttt
      841 agagaaatat atgtccggta aatcattaga agcattggaa ctcgaacaag aggaaagcat
      901 aagatttcag aattgctcct tgtaccctgt ttatcatgga agcgcaaaaa gcaacatagg
      961 gattgagcag cttatagaag tgataacgaa taaattttat tcatcaacat acagaaagaa
     1021 gtctgaactt tgcggaaatg tcttcaaaat tgaatattcg gaagaaagac aacgtcttgc
     1081 atatgtacgc ctttatggcg gaatcctgca tttgcgggat tcggttagaa tatcggaaaa
     1141 ggaaaaaata aaaattacag aaatgtatac ttcaataaat ggtgaattat gtaaaattga
     1201 taaggcttat tccggggaaa ttgttatttt gcaaaatgag tttttgaagc taaatagtgt
     1261 tcttggagat acaaagctat tgccacagag agagagaatt gaaaatccgc tccctctgct
     1321 gcaaacaact gttgaaccga gcaaacctca acaaagggaa atgttacttg atgcactttt
     1381 agaaatctcc gacagtgacc cgcttctaca atattatgtg gattctacga cacatgaaat
     1441 tatactttct ttcttaggga aagtacaaat ggaagtgatt agtgcactgt tgcaagaaaa
     1501 gtatcatgtg gagatagaac taaaagagcc tacagtcatt tatatggaga gaccgttaaa
     1561 aaatgcagaa tataccattc acatcgaagt gccgccaaat cctttctggg cttccattgg
     1621 tttatctgta tcaccgcttc cgttgggaag tggaatgcag tatgagagct cggtttctct
     1681 tggatactta aatcaatcat ttcaaaatgc agttatggaa gggatacgct atggttgcga
     1741 acaaggatta tatggttgga atgtgacgga ttgtaaaatc tgttttaagt atggcttata
     1801 ctatagccct gttagtaccc cagcagattt tcggatgctt gctcctattg tattggaaca
     1861 agtcttaaaa aaagctggaa cagaattgtt agagccatat cttagtttta aaatttatgc
     1921 gccacaggaa tatctttcac gagcatacaa cgatgctcct aaatattgtg cgaacatcgt
     1981 agacactcaa ttgaaaaata atgaggtcat tcttagtgga gaaatccctg ctcggtgtat
     2041 tcaagaatat cgtagtgatt taactttctt tacaaatgga cgtagtgttt gtttaacaga
     2101 gttaaaaggg taccatgtta ctaccggtga acctgtttgc cagccccgtc gtccaaatag
     2161 tcggatagat aaagtacgat atatgttcaa taaaataact tagtgtattt tatgttgtta
     2221 tataaatatg gtttcttgtt aaataagatg a
//
LOCUS       DAXWOO010000412         1111 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.guided.323, whole genome shotgun sequence.
ACCESSION   DAXWOO010000412 DAXWOO010000000
VERSION     DAXWOO010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1111
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.guided.323"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            1..789
                     /gene="aph(2'')-I"
                     /locus_tag="ACGE70_003109"
                     /pseudo
     CDS             1..789
                     /gene="aph(2'')-I"
                     /locus_tag="ACGE70_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
     gene            773..>1111
                     /locus_tag="ACGE70_003110"
     CDS             773..>1111
                     /locus_tag="ACGE70_003110"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8694629.1"
                     /translation="MKLLFIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPGF
                     ESIPRTRFVRI"
BASE COUNT          440 a           96 c          179 g          396 t
ORIGIN      
        1 cttccttttt tctatttaca caaaatattt tacactctcg aaataatcgg tagtggttat
       61 gatagtgtgg catatttagt taataatgaa tacattttta aaacaaaatt tagtactaat
      121 aagaaaaaag gttatgcaaa agaaaaagca atatataatt ttttaaatac aaatttagaa
      181 actaatgtaa aaattcctaa tattgaatat tcgtatatta gtgatgaatt atctatacta
      241 ggttataaag aaattaaagg aactttttta acaccagaaa tttattctac tatgtcagaa
      301 gaagaacaaa atttgttaaa acgagatatt gccagttttt taagacaaat gcacggttta
      361 gattatacag atattagtga atgtactatt gataataaac aaaatgtatt agaagagtat
      421 atattgttgc gtgaaactat ttataatgat ttaactgata tagaaaaaga ttatatagaa
      481 agttttatgg aaagactaaa tgcaacaaca gtttttgagg gtaaaaagtg tttatgccat
      541 aatgatttta gttgtaatca tctattgtta gatggcaata atagattaac tggaataatt
      601 gattttggag attctggaat tatagatgaa tattgtgatt ttatatactt acttgaagat
      661 agtgaagaag aaataggaac aaattttgga gaagatatat taagaatgta tggaaatata
      721 gatattgaga aagcaaaaga atatcaagat atagttgaag aatattatcc tattgaaact
      781 attgtttatt gatgataata tggtttttga tattgataat ttaaaaggat ttcttaatga
      841 taccagttca tttgggttta tagctaaaga aaataataaa attataggat ttgcatattg
      901 ctatacactt ttaagacctg atggaaaaac aatgttttat ttacactcaa taggaatgtt
      961 acctaactat caagacaaag gttatggttc aaaattatta tcttttatta aggaatattc
     1021 taaagagatt ggttgttctg aaatgttttt aataactgat aaaggtaatc ctggatttga
     1081 gtcaatacct cggacacgat ttgtgagaat t
//
LOCUS       DAXWOO010000413         1090 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.guided.326, whole genome shotgun sequence.
ACCESSION   DAXWOO010000413 DAXWOO010000000
VERSION     DAXWOO010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.guided.326"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            11..360
                     /locus_tag="ACGE70_003111"
                     /pseudo
     CDS             11..360
                     /locus_tag="ACGE70_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(632..922)
                     /locus_tag="ACGE70_003112"
     CDS             complement(632..922)
                     /locus_tag="ACGE70_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8694630.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
BASE COUNT          336 a          221 c          166 g          367 t
ORIGIN      
        1 aaatacttaa ttgttaaata ccttatttga tgcaaaagaa atctacgata gtcaacaaaa
       61 aaattttcat tatatccaga aaaatttttc ttagttaagg acctatggat tgcagtaatg
      121 cagaattcgt cgaataaact gccaaagaca tacaatcata ttgcttttaa aattgacgaa
      181 caagatatag attcgtttgt ctcaaaaatc caaatgttag gtttaactgt cgaaccagga
      241 cgttctcgtg tgaaaggcga ggcttcttca atttactttt acgactatga taatcacctc
      301 ttcgagttac ataccggtac tctccaggaa cgtttaaaat cctacaattc cactacctga
      361 gactagtaat gactagtctc tttttatggc ctctgatccc cccattgagc gaatactaat
      421 agggcttcga attgatctgt tcaatcttta agcaatatcc ctcaaatgag tttggaaagt
      481 ttagtgattc atcacaaata cttaaataca tgtagttata tatagattga ggttcttgtg
      541 agcgtatgct cgtgtgggtt caaatcccat cgtctgcatc catctttcca aaacaagcgg
      601 ctaggacatt tttgtctcag ccgcttgttt tctagtttat tctttcatct tgtcggacct
      661 tttttcttgg gtatagactt ttctcatatt ctaccacaat tttgggtttc cctaacacat
      721 gataggatct cacaacaaat tgctttctat tattataata acctgtaacg actaacttca
      781 tcccttcgtc tacttcagct aggaagttca aactatggga agcaatcaaa caattgaccc
      841 catttaaact gaaacggatc aacggtctgg cactaacttg caaagtacgg atcttatcta
      901 ctgttccata ttttttctcc ataaccttca cctcaatacc attatacgaa catacattcc
      961 ctttgtaaag aagaaactct gttttatctt tttgactttt tgtctattac ctttcttaaa
     1021 atcatattgg cacttcctgc gaagatgaag gacatgtact aaaaaaaaga ggcagcagta
     1081 tagatttaag
//
LOCUS       DAXWOO010000414         1073 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.guided.328, whole genome shotgun sequence.
ACCESSION   DAXWOO010000414 DAXWOO010000000
VERSION     DAXWOO010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.guided.328"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            29..>1073
                     /gene="tet"
                     /locus_tag="ACGE70_003113"
     CDS             29..>1073
                     /gene="tet"
                     /locus_tag="ACGE70_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tet(L)/Tet(K)/Tet(45) family tetracycline efflux
                     MFS transporter"
                     /protein_id="HFF8694631.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIKKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTELGRSRPSSI"
BASE COUNT          306 a          161 c          221 g          385 t
ORIGIN      
        1 gaattaaagt gtaaaggagg aattaattat gaagtgtaat gaatgtaaca gggttaaata
       61 caaccaaatt ttaatttggc tttgcgtttt atcttttttt agcgtattaa atgaaatggt
      121 tttgaacgtc tcattacctg atattgcaaa tgattttaat aaaccacctg cgagtacaaa
      181 ctgggtgaac acagccttta tgttaacctt ttccattgga acagctgtat atggaaagct
      241 atctgatcaa ttaggcatca aaaggttact cctatttgga attataataa attgtttcgg
      301 gtcggtaatt gggtttgttg gccattcttt cttttcctta cttattatgg ctcgttttat
      361 tcaaggggct ggtgcagctg catttccagc actcgtaatg gttgtagttg cgcgctatat
      421 tccaaaggaa aataggggta aagcatttgg tcttattgga tcgatagtag ccatgggaga
      481 aggagtcggt ccagcgattg gtggaatgat agcccattat attcattggt cctatcttct
      541 actcattcct atgataacaa ttatcactgt tccgtttctt atgaaattat taaagaaaga
      601 agtaaggata aaaggtcatt ttgatatcaa aggaattata ctaatgtctg taggcattgt
      661 attttttatg ttgtttacaa catcatatag catttctttt cttatcgtta gcgtgctgtc
      721 attcctgata tttgtaaaac atatcaagaa agtaacagat ccttttgttg atcccggatt
      781 agggaaaaat atacctttta tgattggagt tctttgtggg ggaattatat ttggaacagt
      841 agcagggttt gtctctatgg ttccttatat gatgaaagat gttcaccagc taagtactgc
      901 cgaaatcgga agtgtaatta ttttccctgg aacaatgagt gtcattattt tcggctacat
      961 tggtgggata cttgttgata gaagaggtcc tttatacgtg ttaaacatcg gagttacatt
     1021 tctttctgtt agctttttaa cagaattagg cagaagtcgc ccatcctcta tag
//
LOCUS       DAXWOO010000415          868 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.guided.345, whole genome shotgun sequence.
ACCESSION   DAXWOO010000415 DAXWOO010000000
VERSION     DAXWOO010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..868
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.guided.345"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            255..803
                     /gene="aac(6')"
                     /locus_tag="ACGE70_003114"
     CDS             255..803
                     /gene="aac(6')"
                     /locus_tag="ACGE70_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFF8694632.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          310 a          127 c          182 g          249 t
ORIGIN      
        1 attcataatt ggtttttttt aaagcaaaag ataatctata tcatgatgaa caaacagagt
       61 tttttcctcc aattgatggc tttcctgatc tttcctattt tgaataaagt gtttctgtat
      121 aattttttta ttttataagt aagataaata atgaaagaat agagatactg attggtagct
      181 aaagattttt gagcgagaac atttcgtttt ttcaaaagga ctgttagaat aagcaagaac
      241 aagggggaag acgtatgata atcagtgaat ttgaccgtaa taatccagta ttgaaagatc
      301 agctttctga tttactgaga ctgacttggc cggaagaata tggagacagc tcggcagaag
      361 aagtagaaga aatgatgaat ccagaacgaa tcgcggtagc agcggtagac caagatgagt
      421 tagtaggatt tattggtgca atccctcaat acggtatcac aggttgggaa ttgcatccat
      481 tagttgtaga aagctcccga cgaaagaacc aaataggtac tcgattagtc aattacttag
      541 aaaaagaagt agcttccaga ggaggaatca cgatttattt aggtacggat gatttagacc
      601 atggaacaac gttaagtcaa acggacctgt atgagcatac atttgataaa gtggcttcca
      661 tccagaacct tcgtgaacat ccgtatgaat tctatgaaaa attaggttat aaaatcgtag
      721 gtgtcttacc aaatgcaaat ggctgggaca aaccggatat ttggatggca aaaacgatta
      781 ttcctcgacc aaattctcaa taataaaaag aggttgtgac agaagtaatc agcttcaaga
      841 aataagaagg aatttacgaa aattactt
//
LOCUS       DAXWOO010000416          514 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id34
            SAMN40749856-rid21412993.guided.375, whole genome shotgun sequence.
ACCESSION   DAXWOO010000416 DAXWOO010000000
VERSION     DAXWOO010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749856
            Sequence Read Archive: SRR28555143
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134753.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134753.1
            Genome Coverage       :: 96x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 18:08:43
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,052
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 43 of 94
            Pseudo Genes (incomplete)         :: 44 of 94
            Pseudo Genes (internal stop)      :: 21 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749856-rid21412993.guided.375"
                     /isolate="id34"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-11-11"
     gene            39..137
                     /locus_tag="ACGE70_003115"
                     /pseudo
     CDS             39..137
                     /locus_tag="ACGE70_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            138..>514
                     /locus_tag="ACGE70_003116"
     CDS             138..>514
                     /locus_tag="ACGE70_003116"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8694633.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLK"
BASE COUNT          212 a           40 c           91 g          171 t
ORIGIN      
        1 gattctacca aatcgtgtcc atgaaatcat cctaacatca tcctagagct tgccatgtat
       61 atgaaaaatt aggtggtaaa aatgattata aagatgaaat agtatatgta tatgattatg
      121 aaaaaggtga taaataaatg aatatagttg aaaatgaaat atgtataaga actttaatag
      181 atgatgattt tcctttgatg ttaaaatggt taactgatga aagagtatta gaattttatg
      241 atggtagaga taaaaaatat acattagaat cattaaaaaa acattataca gagccttggg
      301 aagatgaagt ttttagagta attattgaat ataacaatgt tcctattgga tatggacaaa
      361 tatataaaat gtatgatgag ttatatactg attatcatta tccaaaaact gatgagatag
      421 tctatggtat ggatcaattt ataggagagc caaattattg gagtaaagga attggtacaa
      481 gatatattaa attgattttt gaatttttga aaaa
//
